LOCUS       DAOPZO010000001        40741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_837_length_40741_cov_7.812712, whole genome shotgun sequence.
ACCESSION   DAOPZO010000001 DAOPZO010000000
VERSION     DAOPZO010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 40741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40741
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_837_length_40741_cov_7.812712"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..773)
                     /locus_tag="PKI68_00005"
     CDS             complement(12..773)
                     /locus_tag="PKI68_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX52104.1"
                     /translation="MNNAIQIENLALDLNGSPVLRRVSFSVGRGEYVSIIGPNGAGKT
                     TLLRCLLGMYAYHGSAKINGVECRAGDSRALARQVSYVPQTHDIEFPLSVYDFVMMGR
                     YPYLSPLAPAQKRDEEAVERALQITGTAQFKGRTLRTLSGGERQKVYIAAALAQETPI
                     ILLDEPATFLDWRHQSEVMTLLKKINVECGATILAVNHDLNSAAHWSDRIVTLKDGRT
                     FSIGTPQDLIQPAPLEALFETAFIRKETLAPASEQ"
     gene            complement(766..1572)
                     /locus_tag="PKI68_00010"
     CDS             complement(766..1572)
                     /locus_tag="PKI68_00010"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HNX52105.1"
                     /translation="MLKKSIALLAAALLFAGCGERIEPAHNGLRIVAMAPNTAEILYA
                     LGLSNAVVGVSGYTVYPPEAAQKPFVGGTYDPNFEMIVALQPDLVVGLETQKDIQARL
                     KALGIPFLGVAHEHISEIMQSILTVGKACGAEAEAQQLYQSLEKQLAGFSAVQTNAGK
                     RVLVCVGHDESLSRMYVAGKGTFYDELIERAGGINVCTETAQKYPEISPEGLAVMRPD
                     IIIDISPTPGKNVSREWTPYRAVSMTNSYAFIPGPRFVLLLQDFIKATHE"
     gene            complement(1572..3575)
                     /locus_tag="PKI68_00015"
     CDS             complement(1572..3575)
                     /locus_tag="PKI68_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX52106.1"
                     /translation="MKKWIIGAALSGSFFTGVSAETTNTVTKAERIVVTATRSVSDVR
                     NVPGNPSVVTAQDIADGHYTSIPEALEKKSGLFFRNIADNPSQASVDIRGFGGDNPHG
                     KVLVLVNGRKLNRPDMAVINWAQIPMQAVERIEVIRGPNSVLYGDHAVGGVINIITRD
                     GSEIPETELQASAGSYAAYDQNAVTSGSLDGLGYVATAGHQSGDGYRDRSAYETSSGS
                     LRLSGNINELLSAYAEASVVKEQHQLPGALSLAEVKQDRRQAINQDDNADSTYYTFNT
                     GIEVVPTDELIFNLDGGASLKDLQADMPSYGSPWTQPVYYDYKINSYTLSPKVTLLTP
                     VANMDNELIVGSDLAWETLDVKKYTNSVRTLLDTDTKVTKEVIGGYIADTLSLTDHLL
                     LNAGARLEKNKVDVTQKGGYSPYDDGISQTENAWQTSLNWLPTDQVKLFTGVERTYRY
                     PFVDEQAIYSGWGDAFNKDLKPETGVNYETGIEVTPLSNTVFQATVFHTDMKDEIAWG
                     SGKNENLDETTHQGVELHAGYKNDIFALDGFYTWLQSEFAAGSNKGNEIPWVPQNKFD
                     ANLALFLTDAITFNTHMSYIGSMCPLGDNSNSSASKQSDYTLVDLLLEYRLPIKKFET
                     KVFAGVDNVFATKYNFLVTDYGWGAGFYPAPERTYKAGLGVKF"
     regulatory      complement(3628..3682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01689"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The adenosylcobalamin-variant (AdoCbl-variant)
                     riboswitch is found in the 5'-untranslated regions of
                     vitamin B12 (Cobalamin) related genes in bacteria; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF01689"
     gene            complement(3745..4974)
                     /locus_tag="PKI68_00020"
     CDS             complement(3745..4974)
                     /locus_tag="PKI68_00020"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX52107.1"
                     /translation="MPILSEAILKRIGRSYRSSYTLDVLEVLPSGICEGRQDTITNLP
                     VLVNARANGLTEAVRWGEPYIFFLIPNVISWIVPIVKETEVLGGLLGGTVIADSDPYD
                     QLETINHLTTSGCPRKAAEQYVKNLPVWNDRERPQKAAEFLFALTCCELNWDMLMLSE
                     NRKKALRQRQIAEEIHRRKQRATSRTLIDEERTLLSLMKAGDQKGARRELNKTLGALF
                     SHTADIRLIKAHVVEMMGYLVRSALEESPHMSSLIEKNHAWMTKIIDAADFETLSDVV
                     ADALDDFMRNIYLQGHSRTNETVAKLLNYLGDNFHQNITLETLAKEVGLSTFRIAHLV
                     KEVTGKTVLQHVHQLRVQEAQRLLEQSDMNCTDIAYETGFGDQSYFIKQFRKWMGITP
                     ARYRRLYRSGKSGQETA"
     gene            5122..5745
                     /locus_tag="PKI68_00025"
     CDS             5122..5745
                     /locus_tag="PKI68_00025"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HNX52108.1"
                     /translation="MARNEELFNAIIKGKRKDVAALVQAEVSAGTNVASILMESMIPA
                     MREIGARFSRNEVYVPEMLIAARAMQAGLDIVDPLLTKSGHEPLGKVAIGTVKGDLHD
                     IGKNLVAMMVKGAGYEVMDLGVDCDVSKYEEAVSKGAQVIMLSALLTTTMPYMKEVVS
                     HFKAKGSSVKILIGGAPVTQEYADEIGADGYSSDANQAVASVEKVLA"
     gene            5792..7174
                     /locus_tag="PKI68_00030"
     CDS             5792..7174
                     /locus_tag="PKI68_00030"
                     /inference="COORDINATES: protein motif:HMM:NF023598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase MtaB domain-containing
                     protein"
                     /protein_id="HNX52109.1"
                     /translation="MTKTIAIDSLSSFVYGRCPKPVKTKRGLELGTGIVFPEVNFTLP
                     AMNINKETWPQVVAHYTEMVDDVIRRSVELEVPQLQIEFETLPDMTLNPAWGLEINRL
                     LADKLIEAQSKHGIKTALRFTPNDIREFSRPPVMRSGIHWEHMLEVFEKSGDAGADFL
                     SIESTGGKELCDEALINADLPGVVFALGVLGCRDMEFLWKHMVAACRRNGVIPAGDSA
                     CGFGNTAMVLADKKMIPKVFAAVVRVATVSRTLVAVEQGAVGPCKDCAYEGVYIKAIT
                     GTPIALEGKSAACAHLSPIGNIAAAVCDCWSNESVQNVQLLSGKAPMVSAEQLAYDCR
                     LMNVAARRSPEDALRLRDWLVESDAPLDPQAYVLHPEVVLRISRRIVQGATAYERTRI
                     AVQAALEELRKGANEKLYRLDPREESWLDMMEMAIENDIPETEAEMIAQQAPLVDKSK
                     CSLTEYGIEA"
     gene            7158..8066
                     /locus_tag="PKI68_00035"
     CDS             7158..8066
                     /locus_tag="PKI68_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNX52110.1"
                     /translation="MESKRKEKIPCHLIAGPLGVGKTTAIRRFVAQSSGFTAVIVNDF
                     GKTGYDAAFIAEAGGAERLRVENIPGGCLCCSSVAQLLPALEMLCARPEVERIIIEPS
                     GIALIDPLLELLRYAAPRCGLDVAPVIVLFDPAKTRPAAIKLIPYWNRLADCAGIIVA
                     NRCDLAPTEAVTQFSDCMKQWNPPKLKVIETSHGELPPELFELRGHTVPAAGNAHPHA
                     DLPPAGAFCSDGTFRLAALLELLEELAPQLDRFKGVFRTDQGWQRLEIAAGHINSRPA
                     VEAAQTSAEWIGGTDRLAERIRHCRI"
     gene            8208..9269
                     /locus_tag="PKI68_00040"
     CDS             8208..9269
                     /locus_tag="PKI68_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNX52111.1"
                     /translation="MQKQFYLDLADRGLRLPIGTHLILHEKANHDTIIHHGDQLGAVM
                     IEAAQYYKSPLAIPLMDLTLEKEAILAILGVPAEEREKFHFDKLPAADAVEKISTAPF
                     MIPRMEATCEAVRYVAEREQQENIVALGMGIGPFSLMTKMLSDPITPVFMAGTGMTAE
                     DDDDVALVECVMELSTQIILRYLTEQAKAGAKAIILCEPAANLVFFSPNQLAEGSDVF
                     ERFVMQPNLKIKAKLDELGVDLIFHDCGELTDGMVESFNRLNPVMLSLGCSRTLWKDA
                     ALLSKDIVLYGNLPSKKFYSDEAVSVADVERMTRELITNMKTAGHPFIMGTECDVLSV
                     PGHEESIKRKVDAMMSCSV"
     gene            9318..10940
                     /locus_tag="PKI68_00045"
     CDS             9318..10940
                     /locus_tag="PKI68_00045"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HNX52112.1"
                     /translation="MENRCGVLLSDLAAEAGLPLNMQCGGQGACKGCLVKLGPGRYRI
                     AETETVVADGQRLEARACKTVVLDERVEIEIPERSLLKIGECAAADFYATRKNLFHPH
                     KNAAGQLGIAVDIGTTTVAAVLVELSTGKILARETAYNRQIELADNVASRIALCTDDA
                     SLKKLQSLIIQGTLVPMFGKLAVQSSRSLEEVTGIVFSGNTVMSHLALGLSALSIGMI
                     PFEPLTKVFSEHAAPEIGLAGCPKARVRTVPAIAGYVGGDLVSDLYMSEPDPARLELI
                     VDIGTNGEIILNDRGQLMATATAAGPAFEGAGLLHGCRAAAGVIEKIICHPDDSIEFE
                     TIGGKPATGLCGSAAVDFIATAWRTGLLNSMGRFDLDRLRAAGRLHQLDCCGTNVNTC
                     VIVPREKSGTDEAILITEFDVSQIMKAKAAVYAGIRTLLERGGHAVGDVERLVLAGGF
                     AAHLRIENAITIGLLPEIPLEKYDVVGNGSLAGAYAALGEGGVWDELHRIAALPGSCH
                     LAETKEFENRFIDALAIPNLDPDEFPVTMSSL"
     gene            10937..11812
                     /locus_tag="PKI68_00050"
     CDS             10937..11812
                     /locus_tag="PKI68_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNX52113.1"
                     /translation="MKITDAVKNGRILVSDGAWGTFLQQKGLKPGECPELWNLERPDD
                     VRAIAASYIDAGADMIETNTFGGNRFKLAHYGLTERVAEINEAGARLSREAAGSDHWV
                     IASMGPTGEILIMEEVSEQEIYEAFKEQAVALERGGADAICIETMSALDEAELAVRAV
                     KENTGCEVICTFTFERTVNGDYRTMMGVSPEQFAAAILAAGADILGANCGNGMERMID
                     IVRALRAAAPAAPILVHANAGLPKNVNGVDTFPESPEEMATRGPALIAAGANIIGGCC
                     GTTPRHIAALKQAIR"
     gene            12516..14453
                     /locus_tag="PKI68_00055"
     CDS             12516..14453
                     /locus_tag="PKI68_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNX52114.1"
                     /translation="MKKIISLITGVLLAASWGHSAENESVRHQMLTAIDQSIEKGSFK
                     PTWESLSKHIDPEWFQDAKFGIYTHWGPVTVGSTGGGSQWYGKAMYEPGNKTFDYHRQ
                     HFGDQNKVGFKDLVPKLTAQKFNADEWADLFARSGAKFAGPVAVHHDNFMMWDSKLTR
                     WTSVNMGPHRDITGELEKAIRARGMKFITTFHHGFAWRYFEPAFKYDAADPQYADLYT
                     EIHEPNAPPSKAFQDQWLALVYEVLGKYQPDLIWFDFEFFRVIQPAYQQKLFAMTYNW
                     ANENNRTIGVCQKSPEIRNYTGIFDIERGRENSIKPYVWLTDSMVSDWFYRESSEAKS
                     VHKLISMLVDIVSKNGCLLLDVGPTVDGTIPDKDRDALLGMGAWLKINGEAIYATRPW
                     KIYGEGPTRLAKSGTFGEDAEKPYGAQDIRFTQSKDGKTLYATALGVPENRQIQVKSL
                     SAAAGKISRVELLGHTGALEWKQTAESLVVTLPATGTYQNAISLKIHAGELNPVSVIY
                     DTSIKANESGNIELSATAAEIHTADKHTPEAKKPLIGSWTDPKDFVSWECTIEYPGTY
                     EVDVTYSCATQPGSAFYVEMDRQKLDGKTASTGMLTYRTDHLGVLYVEKPGLHTINFK
                     AKAEPEWHGIGLSTIVLKRIM"
     assembly_gap    14473..14572
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14598..15671)
                     /gene="arsB"
                     /locus_tag="PKI68_00060"
     CDS             complement(14598..15671)
                     /gene="arsB"
                     /locus_tag="PKI68_00060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNX52115.1"
                     /translation="MNEGITKRLSFLDRFLTLWIFLAMGLGVLLGYFVPSFGVWISSL
                     RPEGTQTSIPIAAGLILMMYPPLAKVKYEELGDVFRDRKILGLSLLQNWVVGPLLMTA
                     LAVVFLHNQTEFMIGLIMIGLARCIAMVIVWNDLAKGDAEYCAGLVAFNSIFQVFFYS
                     LYAWLFVTKLPPLLGLSGAVVNVTIRQIAVSVLIYLGIPFIAGMLSRFFLVRAKGREW
                     YHAQFIPKISPITLVALLFTIVVMFSMQGQKIIARPGDVVLIAVPLLIYFVVMFLVSF
                     WMSAKAGANYAKSATLSFTAASNNFELAIAVAVGVFGINHGAAFAAVIGPLVEVPVLI
                     GLVNVSLYIRKKYFNQTGLPAGR"
     gene            complement(15668..16111)
                     /locus_tag="PKI68_00065"
     CDS             complement(15668..16111)
                     /locus_tag="PKI68_00065"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019835552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNX52116.1"
                     /translation="MKKLKILFLCTGNSCRSQMAEGWARALKSDVIEAYSAGIETHGL
                     NPKAVQVMAEAGVDISGHKSENVADLLHIPFDVVVTVCGHANEHCPLFPGQAKILHVG
                     FDDPPKLARELAAQGADDEAQLDCYRRVRDEIKAFIETLPEGLKK"
     gene            complement(16123..16818)
                     /locus_tag="PKI68_00070"
     CDS             complement(16123..16818)
                     /locus_tag="PKI68_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNX52117.1"
                     /translation="MTELFTALTRALDGEPLMALSAALVWGVLSILLSPCHLASIPLI
                     VGFIDEQGRISTRRAFGISLLFATGILITIAMIGGLTAAAGRILGDLGPYANYFIALI
                     FFVVGLHLIGVIPLPSRPGQVNMKRRGALAALILGLVFGLALGPCTFAFMAPLLGVVF
                     KAGAGRPVYAGSLLLLYGIGHCAVIVLAGTFTEVVQHYLNWNEHSAGALILRKLCGLL
                     VLLGGLYLIYTAA"
     gene            complement(16815..>17131)
                     /locus_tag="PKI68_00075"
     CDS             complement(16815..>17131)
                     /locus_tag="PKI68_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNX52118.1"
                     /translation="RPVVSAETPAQKPLPRLVDLGSGSCVPCKLMTPVLDELKTTCAG
                     KLEVVFIDVRDNPQAGQQYGIRTIPTQIFYDAAGKELFRHEGFFAKDEILAKWKELGV
                     QL"
     assembly_gap    17132..17231
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17409..18428)
                     /locus_tag="PKI68_00080"
     CDS             complement(17409..18428)
                     /locus_tag="PKI68_00080"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNX52119.1"
                     /translation="MSKPTNTYIWRGAAGLVLWWLVYRHLAAAAAWLTDTLLGLKSGS
                     HLASAVEFFIFEVPKVLMLLTLVVFGVGIVRSFFTPERTRRMLAGKREFTGNVLAALL
                     GIVTPFCSCSAVPLFIGFVTTGVPLGVTLSFLISAPMVNEIALVLLFGLFGWKVAAIY
                     LVTGVVIAIAAGWVIGRLRMEKHVEAWVYQTQTGDGAPDEELDWPARIRFGLDAVKDI
                     VGRVWLYVLIGIGVGAGIHGYVPENFMASIMGRTWWSVPVAVLLGVPMYSNAAGIIPI
                     VQALLGKGAALGTVLAFMMAVIGLSLPEAIILRKVLKPLLLAVFFAVVAAGIMLVGYL
                     FNLIM"
     gene            complement(18421..18762)
                     /locus_tag="PKI68_00085"
     CDS             complement(18421..18762)
                     /locus_tag="PKI68_00085"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNX52120.1"
                     /translation="MNKPLSALKSLSDRNRLRVVAALMHHDELCACQIIELLQVTGAT
                     ASRHMELLIRAGLVQSRKAGRWVHYRLAADFPAPLRQWLEAELNADGTRKADRRALEK
                     IIAGKPGGRDE"
     gene            18926..20542
                     /locus_tag="PKI68_00090"
     CDS             18926..20542
                     /locus_tag="PKI68_00090"
                     /inference="COORDINATES: protein motif:HMM:NF001964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNX52121.1"
                     /translation="MSFEQEIAKRRTIAIISHPDAGKTTLTEKLLLYGGALQLAGSVT
                     ARKSQRATTSDWMELERQRGISVTSTVLQFEYRGCCVNLLDTPGHRDFSEDTYRVLMA
                     VDSVVMVIDAAKGIESQTRKLFEICRMRGIPIFTFMNKLDRPSRNALSLMDELEQTLQ
                     LRVCAMNWPLGNGSDFQGVYDRQAKQAHFFERTAHGAFRAPVALHSVTDSQVRDRMPA
                     EVYQELINELEMLDTAGEVFDLPEVQAGDLSPVFFGSAINNFGVQLMLDAIVDYAPAP
                     QPRFSGDVKVLPENPKFSGFIFKIQSNMDPNHRDQIAFLRICSGCFTRDMRVTHSRTG
                     ELVRLSSSHRVFARDRETVDVAYPGDVIGLVGHSEFGIGDTLTEDPSIIYHEMPQFAP
                     ECFAMLYNPVSAQYKRFQAGVKHLLQEGALQSFHYKDPAQNVPLLGAVGVLQFEVFKY
                     RLENEYGATARLEPAPYKFFRWVDESGGEKSVVTESMLPPSCRMAFDSLDRKVVLFDS
                     EWILKFFCERNPEVKLISLPVSANLPSGGR"
     gene            complement(20539..20940)
                     /locus_tag="PKI68_00095"
     CDS             complement(20539..20940)
                     /locus_tag="PKI68_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX52122.1"
                     /translation="MAHILVVDDEEGITMVIKEMLVRLKYDVLTAKNGKEALEKLKLG
                     PVELLITDIVMPDMDGLELIEEVQRTLPKVKIIAISGGGNKFYPGDYLNRAKELGAVY
                     CLPKPFMLNELAAVVAKALSSGQPANSNRPD"
     gene            complement(20964..22217)
                     /locus_tag="PKI68_00100"
     CDS             complement(20964..22217)
                     /locus_tag="PKI68_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52123.1"
                     /translation="MKTFASSKKNGAVLVAVMVVLLAVTLMVAALLQLGAFGQIETVQ
                     QLRSAQAHWLAEAGLERALSRVMASRDYRAILPDSFSNDETLLNGFGGYDVEISKVYS
                     NALLDTYIITSTGRVSNDAVSATGVVRLRMVAGPGGQQALMGLDGSQSTIAANVTVNG
                     TIYQDGVLHIASGASTELNDIIDATGGITGNTGEATPGDLPDPIPGPRIEASRLTAYA
                     NMLGYAASTNAGAYPSGSAISLGGGTNYYNGSATIDKNVTGPGTIVVASNLTVAADIP
                     ANVNLIVGGIVTLGNKSALYGNNIVFTYRDIDISGGNFYGSFVSLLSMGGIWISAGID
                     NFKGILYADGLYRSPAQDGKSYGIIFKSGTQTIRGTVIAWEGFYMGSNATITYDPTVF
                     ANPNPINYGNTLAIQKKTWEEAPYN"
     gene            complement(22220..22678)
                     /locus_tag="PKI68_00105"
     CDS             complement(22220..22678)
                     /locus_tag="PKI68_00105"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNX52124.1"
                     /translation="MKTDRQKGVTLIELMVTIAAASILMLMVALILMMAFQSWRINNA
                     YADLRRDAALAFVRMVKDVREADYTGLTAGNPLTLPSAVTNKTVLYNQPTGGKTLLYS
                     DGSGSSIIIPDNVRVFTSVKQDDGVLLSLELANTNFNIAITNEVFVNTRN"
     gene            complement(22683..23096)
                     /locus_tag="PKI68_00110"
     CDS             complement(22683..23096)
                     /locus_tag="PKI68_00110"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNX52125.1"
                     /translation="MRTELFRRKKRDGMTLIEIILALAVLAVMALAALISFSYARYLA
                     VTSDLEQSAIHAGTSDIERRLYNPASPAPQGISKTAGWTIETTDITTGITTNTEYYGT
                     DAYHYLVISNTVTYRDGKTVEFVTYRSLEVPSSER"
     gene            complement(23093..23575)
                     /locus_tag="PKI68_00115"
     CDS             complement(23093..23575)
                     /locus_tag="PKI68_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52126.1"
                     /translation="MKAPSRTAIILSSLLTGLLLSAVFFWRSETNSRRYAGNNGYFEQ
                     KYQLPVKTLRLAQDAVKESFHNNGTGLVEIKNPAPVSAAKEAGLQQAAQPSEFSGPVK
                     LQGISWAQEKPVAMISGRVYQTGDRFGDCTVQEIRTRSILLRDAGGTDIEIKLMEKNP
                     "
     gene            complement(23572..24111)
                     /locus_tag="PKI68_00120"
     CDS             complement(23572..24111)
                     /locus_tag="PKI68_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52127.1"
                     /translation="MDTACFLRKPSVLALWLIAPLALTAVAGLVSRSILEHARSTYAQ
                     SKALEQLIPELSKGLSEFALFAATYRIESDAAQFEDRHIALLNAAAEHIDFNITAINI
                     TQDPPDKAPEGTVRINILIKGTGSGNGIYAFLNNVHVRDPFAYEKRVQIMPDGTDSGG
                     FALEAELSVIQIAQKGAAQ"
     gene            complement(24116..24715)
                     /locus_tag="PKI68_00125"
     CDS             complement(24116..24715)
                     /locus_tag="PKI68_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52128.1"
                     /translation="MKQFEINLARTLIMPVAKRRLIYRGMLIYLLVSAGILTFSCWKA
                     CSDIQEALNYLHRNRAIQGQFHSLYTGSGSMAQYADRLQKQVAERTRQATAIKEALPA
                     DIHTVLPLLKVLVSQTDGSTLHRLTFIQKNDSRPAFEFSIAMPTNGRKSPGAGFLQTW
                     QKNPELAQCFASITPATTSRGKSGNEDVFIMNYKVSFKE"
     gene            complement(24702..25649)
                     /gene="pilM"
                     /locus_tag="PKI68_00130"
     CDS             complement(24702..25649)
                     /gene="pilM"
                     /locus_tag="PKI68_00130"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNX52129.1"
                     /translation="MKTRLKRLFGCEEDVIGLDINYGRVVAAHFSNAGSGFQMDRLAA
                     GEFPVEFTDQQLAKWLRVFWKKEKLPGRTVRTCLHSRSLIVRYFNYKNLTTDELAQTL
                     RLEAEEALQKPANELCFDWHVNPVETARKSVELSGFLAAVPRKTITRHLNLMRAAGLY
                     SIHVEIGCSALYNFYTRLRDETDTSPVCLINLADRTADIIMLSNSGIYPRTLFSANDS
                     WDKNLGYLMENIQNALLYYHLKTNQHPMEKIVLIGRIPDKENFSRTLAKNTALPVNIL
                     NPGADPRIAVDRLNLSIPETFNVATGIGLALGGLCDETV"
     gene            complement(25724..26149)
                     /locus_tag="PKI68_00135"
     CDS             complement(25724..26149)
                     /locus_tag="PKI68_00135"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNX52130.1"
                     /translation="MRTKNKKSGFTLVELMVVAAIIAILAAIIIPLMANNRERAIATE
                     GQNICSTVATAAKVYYAEKGTWPSFAQLPQIVRNEVGNAKYFVGSGVTIGGTYDAYTI
                     TATAAANAGGLSGKTLTLNQAGTWGGTMVGTGSGQVDIQ"
     gene            complement(26225..27523)
                     /locus_tag="PKI68_00140"
     CDS             complement(26225..27523)
                     /locus_tag="PKI68_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNX52131.1"
                     /translation="MNKFNYTAKNTAGDLIRDMIEAESRQSALTTLRQKGLTVVSLTD
                     VEPVLPVSAETAKSKDVGGQLFRRRSKEQSGDLQLAPKKNPGSKKIHLTDMAVFCRQL
                     AISVNSGLPLREALEGIHEDMDQPALKAVLANIIKKLYDGIPFSSAVAAHPKVFSPVF
                     IGMVQAAEQSGTLGETLNRLAGYIESSDKLRRKIKSLMAYPIFVAAFFIIICLVMVVA
                     IIPRFQDIFNDLHAKLPALTRGVFGLNNFILQNVPLILAFAVLLTVVFIFCRRIPSVR
                     LWLDRTKLKMPITGVCLKRYILARICRCTAIMLKSGVPISTTLRIVSAIGDNLIIEQA
                     IVQGQAQIISGSNIADGFKKTGIFPSLLIRMLRVGESAGRLPDVLDRVADAYEDQVEA
                     SIVTATALLEPVIISVFGLMVLVLVLSIYLPVFTVSTHIR"
     gene            complement(27537..>29198)
                     /locus_tag="PKI68_00145"
     CDS             complement(27537..>29198)
                     /locus_tag="PKI68_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNX52132.1"
                     /translation="KIEQLRKVFLDHKDKDGALQRLAAECGLTSSRELALALSNLLHI
                     PFVEIPEEYHIERSVIRLVPEATARRYTLIPFEKENESSLKVIMANPLDLDAVDTVRS
                     QTKLEIHQVVGVEEDILALIARSYKEEAYIEEGLQDIVSLEDVDLEVEIDDQVNVELD
                     QLKDKANDVPVIRFVNLLLLEAIRDRASDIHFEPAEKYVAVRFRVDGVLREVTPPPRA
                     IYAAIATRIKILSELDIAEHRLPQDGRFKFKVHDRVIDVRVSVLPVAHGEKIVLRILD
                     RASLLVDMKDVGFDPPMLKEFRRILGLPNGIILLTGPTGSGKTTTLYSALSFLKNPEV
                     NIQTVEDPIEYLIDGINQTHIHPKIGLDFANCLRSILRQDPDIIMIGEIRDLETAQIA
                     TRSSLTGHLVLSTLHTNDAPSSFSRLKDIGVPAYLISATVKLVIAQRLVRMICPRCKE
                     EYLPPPEDIQLAEQLCPGAAKWTFFRGSGCPDCQQTGFLGRTGIFEFLEVSDPLREMI
                     VEHCSDTVLRKAALESGMESLAHNGMMKVKNGITTLDEVLRATQF"
     assembly_gap    29199..29298
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<29299..30434)
                     /locus_tag="PKI68_00150"
     CDS             complement(<29299..30434)
                     /locus_tag="PKI68_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52133.1"
                     /translation="MNRPIQFKPPVLLALMLAGSLFAGAFGQSTTTAAGSTVTPPAAA
                     TDYVSIQVDGGTILQVLNAFAIQTGRNVVVGPEVVCEGVNIHLNRVRWDEALEVILKP
                     YGFGYRKVGDTIVISKLEKLTELAAVEPLETKVFELRFLDAADVQEMLAKQLSPRGTM
                     SVMTVRGQKGWEFATRSGGNSGQRSAYGNLGKLERAMGKADAADQGKSKTLIVTDVPS
                     SLTRISAMLDKLDVIPQQIVVEARFMEVNNNLLRDLGLDFTYAKSEYSVGQILSTVAP
                     GNFDPTSTGFWPNSLTPLNQNAFLTRNGQSLDVAIRMIQEDKDTKVLSAPRVLTMNNQ
                     EATIIVGQKFPIIESNVSGGSGNNNTSTTLDYYENIGIQLNVVP"
     gene            complement(30431..31519)
                     /locus_tag="PKI68_00155"
     CDS             complement(30431..31519)
                     /locus_tag="PKI68_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNX52134.1"
                     /translation="MEPNSITKLLEQVRDQKASDLLLSAGAPPQIRINGLLHKVSEEP
                     LTGNDLKILAEELLNDRQRLILESYKSIDFSYDFPGISKFRFNIYFQRGHVALAARII
                     PDQIPSFSALGLPRIVEEFANHSSGLFLVTGPAGSGKSTTLAAMVDHINRNKKLHVIT
                     IEDPIEYEHNHGLCIVDQRQVGSDTESFTDALHSIFRQSPDVIMVGELRSLETIRLAL
                     TIAETGHLILGTLHTQDTVHAISRIVDVFPAEQQRQIFFQLSQVLIGVISQQLIMTQD
                     QSRRVLACEVMRVNSGIRNLIREMKVEQIYSMVQAGRSEGMITMNESLQELLALDLIR
                     PQAALSRTAKPKELLRIIEAHSNERKRK"
     gene            complement(31642..32319)
                     /locus_tag="PKI68_00160"
     CDS             complement(31642..32319)
                     /locus_tag="PKI68_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX52135.1"
                     /translation="MPAARSSRIIVVDDHPVVCNGLCRLVADDSRLSICGTTDNAGEC
                     ISLVEKTHPDMLLLDISLKGSDGIELTKKIRQINPHIVILIFSMHDEELYAERALNAG
                     ANGYVMKQEDPDLLLKAIHQVLGGEIFLSPAMTGRLLRRISEGHIPKSGVLKQSIEKL
                     SDRELEVFELIGKGMSTRRIAEALNLSVKTVETYRMHIKEKFDLNDAAELAHHAVHWV
                     EVGLRTR"
     gene            complement(32334..33026)
                     /locus_tag="PKI68_00165"
     CDS             complement(32334..33026)
                     /locus_tag="PKI68_00165"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNX52136.1"
                     /translation="MGETGSVFSGVCCHEVERQMLELWDTEKRRIGHDLHDGVGQQMT
                     GIGLLSKALEHRLRESGNPEAEEVHKIVELADEVVNRIREVVAGMAISEIQDQDAATA
                     LCHLCQRIEQCYQIRCVFSNRLTALPITDPDMVKHLYLIAAESIHNAIRHGEADQIEV
                     RLCQGTGSNQGELVIQNNGNCVPDDFEKSSGIGLCGMRLRAQALNGCLNIEQYSNHTV
                     AVRCLFSPINGA"
     gene            33281..34312
                     /locus_tag="PKI68_00170"
     CDS             33281..34312
                     /locus_tag="PKI68_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNX52137.1"
                     /translation="MLNRLMGLFSSDIGIDLGTANTLVYVRDRGIVLREPSVVAVQAG
                     TNRVLAVGDEAKRMLGRTPGSITAIRPMKGGVIADFEITEAMLRYFIQKVHNRRRMVR
                     PRVVVAVPSGITEVEKRAVKDSAVHAGARDVFLIEEPMAAAIGVGLPVQEPAGNMIVD
                     IGGGTTEVALISLAGIVYSRSVRIGGDDLDEAIIQYLKREYNLLIGERTAEDIKISIG
                     SAMLIGEETTMEVKGRDQVAGLPKTLIISSEEVRGALKDPVSGIVDAVRVTLDRCPPE
                     LSSDLVDRGMVLAGGGALLRGLDKLIADNTGLPVHIADDPLSAVAEGTGVVLHEINFL
                     RKLSEARAR"
     gene            34376..35206
                     /gene="mreC"
                     /locus_tag="PKI68_00175"
     CDS             34376..35206
                     /gene="mreC"
                     /locus_tag="PKI68_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNX52138.1"
                     /translation="MVERKSFLKWAIAVAALLLLFNLPGGCTARIKGIFKDPVSPVET
                     FFIKTAHSLKAGADTVRGFGGVMEKNSRLSEEVIYLQAKLVGLENLEQENRHLQDQLD
                     FYKRQKHTLIPCQVTARTISGWWQSMRLDKGRTQGVEAGRAVISPDGLVGRTDEVSAY
                     TAEVLLISDPACKVSARVSRTGSFGLVTGLGVNAQGYPVARMQFIHKDSPVRAGDAVV
                     TSGLGGVFPKDILIGYIETVGMEEAGLYQYADIIPKAVTELMDVVFVTGEEENAEAEE
                     "
     gene            35203..35706
                     /locus_tag="PKI68_00180"
     CDS             35203..35706
                     /locus_tag="PKI68_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52139.1"
                     /translation="MSRRFITVLLAAGTVLQTVLPSWNAFGALELPLLTGLIIFIALH
                     TDRARMFYAAVLAGLLHDSFCPAPLGLSIPFFVILAAGVNWIRDEVFGDQPATYAVLG
                     AVAAVFELLYYASIFKLSGLRPMSGGLLALRLAGGLMAGTVVVPLVALTVEQLDRLIP
                     RNRRRYI"
     gene            35703..37523
                     /gene="mrdA"
                     /locus_tag="PKI68_00185"
     CDS             35703..37523
                     /gene="mrdA"
                     /locus_tag="PKI68_00185"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNX52140.1"
                     /translation="MMRVRRTDRHYIPRVYLLTGLLVCAFLLLGVSLWRLQVAKVSQF
                     ESRQQIQSLRRVRLPGIRGKIYDRNGLCLADNRPVYSIALFLEDMRRPGPWSKTIDRA
                     MDVVGQVSQITGLKPVMDRDGIWKHIRLRLPLPLVLWNDIGPQSMARLAELGCDIPGV
                     DIYTQATRIYPYSPYTSHLLGYVGRADIDAMSEEESYSYYLPEMTGRAGLEKLFDPFL
                     RGEAGGKLVQIDVSGYRHNELAHRTPKPGGDLRLTLDMEIQLLAHKALGTHRGAAVVL
                     DPNNGDVLAMLSTPGFDANRFVPYINTADWKALSEDPGKPLLNRAVAGVYPPGSIFKP
                     LVALTAATLNPAAINVVYDSPSVFRIGSRVVHTTGHGEVDMRQAMKFSANVYFFKTAL
                     ACGPEPIIQQALAAGLGQKTGVEVDFESSGTIPDEKWQKDNRTGWSDGDTCNLAIGQG
                     YVTATPIQMAMLTATIANGGYLYRPRMVQAYRQPGSEDYVLNPNHRIGKMNWSAAALR
                     AVRNGMRDVIMEKDGTGHRAAVEGFEFAGKTGTAEYGLKEEGAKHTWMTAFAPLDNPQ
                     YAVAILIEDGDTGGLTVGPCLKVLMEGLYAKMKREGRLQS"
     assembly_gap    37627..37726
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <37727..38603
                     /locus_tag="PKI68_00190"
     CDS             <37727..38603
                     /locus_tag="PKI68_00190"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNX52141.1"
                     /translation="LVLVLFIGMSVYGAHRWFSFGSVTVQPSEFAKLAVIFTLAAWLG
                     DPATDIERFKTFILAFLLTGIPFLLIAAEPDLGSAMILVPIVMVLLFCAGVPLRPLLS
                     LVGIGVAAVLVLVVWLLFFPDSCPLEDYQKNRILVYLNINQDPLGSGWNKLQSQIAVG
                     SGGWFGKGYLKGTQNILGFLPRTVAPTDFIYSVVAEETGFAGSSLLLALYTMVVVRCM
                     HAAIVARDMFGRLLSAGIGVLIFSHVFVNIAMTIGLMPITGVPLPLMSYGGTFMVSIM
                     TALGLVQSVCLRRRI"
     gene            38620..40215
                     /locus_tag="PKI68_00195"
     CDS             38620..40215
                     /locus_tag="PKI68_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNX52142.1"
                     /translation="MLSISAIKEKLLAGSRKKKEILINIEPLETRVAVLDERKLDNFH
                     IERSEESRLVGSIFKGKIQNLEDGLQAAFVDIGMQKNAFIHYWDMIPEDAARLEEQEG
                     SRNSARRRRKFSPGEMSKKFPIGSEIVVQVTKDAIGTKGPRVTANLSVPGRYLVMMPG
                     TGLKGISKKIGDAKERVRLKKILARLPIPDTIGIIVRTAGSGTRKTSFVRDARTLIEI
                     WKEIDSGMREKPAPCVLYKEPNLAERVVRDYLTEDIDRVYVDDREAYEMIRKMIAKYS
                     RRARNWVQLYAGEEPIFDYFEVEKQIESVFRRKVWLKSGAYLIFDETEALVAVDVNTG
                     RHKGGKSAEESILSVNMEAAEEVARQLRLRNVGGLVVIDFIDMKSKRDQNLVYKTMKD
                     ALREDRARTNVLPISQLGLLEMTRQRFEESIYTTTYVECDYCGGRGRVKSALTMSVEL
                     QRRLSAALRKDRKGIHSMKITVNPTVLNRLRHEDEQTLIDMEKKFQGHLTFVSDPHMH
                     MEAFTITNEQTHQVVYSSIEIDK"
BASE COUNT         8830 a        10949 c        11338 g         9224 t
ORIGIN      
        1 gaatttcagt ttcattgctc ggatgccggg gcgagggttt ctttgcggat gaaggcggtt
       61 tcaaaaagtg cttcgagcgg cgcaggctga atcagatcct gcggagttcc gatggaaaac
      121 gtccggccgt ctttcagtgt aacaatccgg tcgctccagt gcgcggcact gttgaggtcg
      181 tggttgaccg ccagaatggt ggcgccgcat tcgacgttga tttttttgag cagcgtcatg
      241 acttcggatt ggtgccgcca gtcgagaaac gtggccggtt cgtcgaggag tatgatcggc
      301 gtttcctgag ccagcgcggc ggcgatgtaa actttctgcc gctcgccgcc ggagagtgtg
      361 cggagcgtcc ggcctttgaa ttgcgcggtg ccggtaattt gcagtgcccg ctcgaccgct
      421 tcttcatcgc gtttctgtgc gggagcaagg ggtgagagat acggatagcg gcccatcatg
      481 acaaagtcgt aaaccgaaag gggaaattca atatcgtgtg tctgcggtac atagctgacc
      541 tgccgcgcca gtgcgcggct gtcacccgcc cggcattcga ctccgttgat ttttgcggag
      601 ccgtgatagg cgtacatacc gagcagacag cggagcagtg tcgtttttcc cgcgccgttc
      661 ggaccgataa tggaaacgta ttctccccgg ccgaccgaaa acgagacgcg gcgcaaaacg
      721 ggcgaaccgt tcaggtccag cgcgaggttt tcaatctgga tggcgttatt catgagtcgc
      781 tttgatgaaa tcctgaagga gcagaacaaa acgcggtccg ggaataaagg cgtaactgtt
      841 ggtcatcgac accgcgcggt aaggcgtcca ttcacgggag acatttttcc cgggggttgg
      901 agagatgtcg atgatgatat ccggcctcat gacggcgagg ccttccggcg agatttccgg
      961 atatttctgt gcggtttcgg tgcaaacatt aatgccgccg gcgcgttcaa tcagttcgtc
     1021 gtagaacgtt cctttgccgg caacatacat ccggctcagg ctttcgtcgt ggccgacaca
     1081 aaccagcacg cgctttccgg cgtttgtctg aaccgcagaa aaaccggcga gctgcttctc
     1141 cagtgactgg taaagctgct gtgcttcggc ttccgcgccg caggctttgc cgacggtgag
     1201 aatcgactgc atgatttcgc tgatgtgttc gtgcgccacg ccgagaaacg gaatgcccag
     1261 cgctttaagc cgggcctgaa tatctttttg cgtttcaagt ccgacaacca ggtcgggctg
     1321 aagagcgacg atcatttcaa aattcggatc atacgttccg ccgacgaacg gtttttgcgc
     1381 cgcttccggc gggtaaaccg tgtagccgct gacgccgacg accgcgttac tcaagccgag
     1441 tgcgtagaga atttctgcgg tgttcggcgc catcgccaca atgcgcagtc cattatgcgc
     1501 cggctcaatc cgctccccgc accccgcaaa aagcagggct gcggcgagca gcgctatgga
     1561 tttttttaac attagaattt tacgccgagg cccgctttgt atgtccgttc cggtgcggga
     1621 taaaaaccgg ctccccagcc gtagtcggtg acgaggaaat tgtattttgt cgcaaagaca
     1681 ttatccacgc ccgcgaaaac cttggtttcg aattttttga ttggcagtct gtattcgagc
     1741 aggagatcga cgagggtgta gtccgactgt tttgatgcgg aactgttgct gttgtcaccc
     1801 agcggacaca tgctgcctat gtagctcatg tgcgtattga acgtgatcgc atcggtcaga
     1861 aagagcgcca gattggcgtc gaacttgttc tgcggaaccc acggaatttc attcccttta
     1921 ttggagccgg cggcgaattc cgactgaagc caggtataga aaccgtcgag tgcaaaaata
     1981 tcgttcttat agccggcatg cagttcaacg ccttggtggg ttgtttcgtc gagattttca
     2041 tttttgccgg aaccccaggc gatttcgtct ttcatgtcgg tatggaagac ggttgcctga
     2101 aagaccgtgt tggaaagcgg agtaacttca atgccggtct cgtagttgac gccggtttcc
     2161 ggtttgaggt ctttgttgaa ggcgtcgccc cagccggaat agatggcctg ttcgtcgacg
     2221 aaaggatagc ggtaggttct ctctacgccg gtaaacagtt tcacctgatc ggtcggcagc
     2281 cagttgagcg atgtctgcca ggcattttcg gtttggctga ttccgtcatc atatggcgaa
     2341 taaccgcctt tctgtgtaac gtcgacttta tttttttcaa gtcgcgcgcc agcgttcagc
     2401 agaagatggt cggtcaaact cagagtgtcg gcgatataac cgcctataac ttctttggta
     2461 acttttgtat cggtgtccag taaagtgcga actgagttgg tatacttttt gacgtccagt
     2521 gtctcccagg caaggtcact gccgacaatc aattcgttgt ccatgttggc gacgggtgtc
     2581 aacagagtga ctttcggtga aagggtgtaa ctgttgattt tgtaatcgta gtataccggc
     2641 tgagtccaag gagatccata tgacggcata tcggcctgaa ggtctttcag ggaagcgccg
     2701 ccatcgagat tgaagatcag ttcgtcggtc ggtacgactt caatgccggt gttgaacgtg
     2761 taataggtgc tgtcagcgtt atcgtcctga ttgatagcct gacgccggtc ctgcttaact
     2821 tctgccagtg aaagcgcgcc gggcagctgg tgctgttctt tgacgaccga agcttcggca
     2881 taggcggaaa gcagttcatt gatgttaccg ctcagccgga gactgccgga agaggtttca
     2941 taggctgacc ggtcgcggta gccgtcgccg gactggtgcc cggcggttgc gacataaccg
     3001 agtccgtcca gacttccgga ggtgacggcg ttctggtcgt acgccgcata acttccggcg
     3061 gaagcctgca attcggtttc aggaatctct gatccgtcgc gggtgatgat gttgatgacg
     3121 ccgccgacgg catggtcgcc gtacagaacc gaattcgggc cgcggataac ttcgatgcgt
     3181 tcaaccgcct gcatcggaat ctgcgcccag ttgatcaccg ccatgtccgg ccggttcagt
     3241 ttgcggccat tgaccaggac cagcactttg ccgtgcggat tgtcgccgcc gaagccgcga
     3301 atatcaaccg aggcctgtga cgggttgtca gcgatattgc ggaagaaaag accggacttt
     3361 ttctccagcg cttcgggaat ggaggtgtag tgcccgtcgg cgatgtcctg tgcggtgacg
     3421 accgatggat tgcccggcac attgcgcacg tcgctgacgc tgcgtgttgc ggtaacaaca
     3481 atacgttctg cttttgtgac ggtgttggtg gtttcggcgc tgacgccggt gaaaaaagag
     3541 ccgctcagcg cggctccgat gatccatttc ttcattctgc ctttctgccc cttgcgcgga
     3601 ggcgggtttc ggttccggca gaaaggcggt gttccggctt taacggttgc gcgacagcgg
     3661 ctgattctca cagcactttc cctgcacctt ctgcatcagc cgcggaaagt aggggaacgg
     3721 actgtgcagg tcaagctgtt tggtttacgc ggtttcctgt ccagattttc cggaacggta
     3781 aaggcggcgg tagcgtgcag gagtgatgcc catccatttg cggaactgtt taataaaata
     3841 gctttggtcg ccgaatcccg tttcgtaggc gatatcagtg cagttcatgt cgctttgctc
     3901 cagcaggcgc tgggcttcct gtacacggag ctgatggacg tgctgcagaa ccgttttacc
     3961 ggtcacttct ttaaccagat gggcgatgcg gaaggtgctg aggccgactt cttttgcgag
     4021 tgtttcgagc gtgatgttct ggtgaaaatt atcgcccagg tagttcagaa gttttgcgac
     4081 cgtttcgttg gtgcggctgt gtccctgcag atatatgttg cgcatgaaat cgtccagagc
     4141 atcggcaacc acatccgaca gcgtttcaaa atcagcggca tcaataattt tagtcatcca
     4201 tgcatgattt ttttcgatga gcgaactcat gtgcgggctt tcctccagag cgctgcgcac
     4261 gagatagccc atcatttcga caacgtgggc tttgatgagt cggatatcag cggtatgtga
     4321 gaaaagagcg ccgagcgttt tgttgagttc tcgtcgcgcc cctttctggt ctccggcctt
     4381 catcagcgac agcagggtgc gttcttcgtc aattaatgtg cggctcgttg ccctctgttt
     4441 gcgccggtga atctcttctg caatctgcct ctgccgcaac gcttttttac ggttttcact
     4501 gagcatcagc atatcccagt tgagttcgca gcaggtcagc gcaaaaagaa attccgccgc
     4561 cttctgcgga cgctcccggt cgttccagac cggaagattt ttgacgtact gttcagccgc
     4621 ttttcgcgga cagccggagg ttgtcagatg attgattgtt tccagttgat catacgggtc
     4681 gctgtccgca attactgtcc cgcccagcag tccgccgaga acttcagtct ctttaacaat
     4741 cggaacaatc cagctgatca cattgggaat cagaaaaaaa atatagggtt cgccccagcg
     4801 cacggcttcg gtcaatccgt tcgcccgtgc attcaccagc accggcagat tggtgatcgt
     4861 atcctggcgt ccctcgcaga tgccggacgg cagcacttca agtacatcca gcgtatagga
     4921 actgcggtag cttctgccta ttcgtttcaa gatggcttcg gacaagatcg gcatataaaa
     4981 aactccattt atttccgaaa acgccatcat ttaataaaat gacaacactc cccagcaaca
     5041 tcattatata tttaatttaa taaacctatt tttattaggc atttcaggta cagtggcggc
     5101 attaaacctt acggaggatc tatggccaga aatgaagaac tgtttaacgc gatcataaag
     5161 ggcaagcgga aagacgtcgc tgcactggtg caggccgaag tcagcgccgg cacaaacgtc
     5221 gcctcaatcc tgatggaatc catgattccg gcaatgcgcg aaatcggcgc gcggttttcc
     5281 cgcaacgaag tttatgttcc ggaaatgctg atcgccgccc gcgccatgca ggccggtctc
     5341 gatattgtcg atcctctgct gaccaagagc ggacacgagc cgctgggtaa agtggcaatc
     5401 ggcacggtca aaggcgactt gcacgacatc ggcaaaaatc tggtcgccat gatggtgaaa
     5461 ggtgccggtt acgaagtcat ggatctgggc gtggactgcg acgtttccaa atatgaagag
     5521 gctgtcagca aaggcgcgca ggtcattatg ctcagcgcac tgctgaccac cacgatgccg
     5581 tacatgaaag aagtggtttc tcacttcaaa gccaaaggct ccagtgtgaa aattctgatc
     5641 ggcggcgctc cggttacgca ggaatacgcc gatgaaatcg gtgctgacgg ttattccagc
     5701 gatgccaacc aggcggttgc atcggtcgaa aaagttctgg cgtaacaagc taaacacccc
     5761 ggataaacgg ggtgttttta tggagataaa tatgactaaa accatagcca tagattcctt
     5821 aagcagtttc gtttacggac gctgcccgaa accggttaaa acaaagcgcg gacttgaact
     5881 cggcaccggc atcgtatttc cagaagtcaa ctttacgtta ccggcgatga acattaataa
     5941 agaaacctgg ccgcaggtgg tcgctcacta cacggaaatg gtcgacgatg taatccgccg
     6001 ttcggtcgaa ctggaagttc cgcagctgca aatcgaattt gaaacactgc cggatatgac
     6061 gctcaatccg gcgtggggcc tggaaatcaa ccgcctgctg gccgataagc tgatcgaagc
     6121 gcagtcaaaa cacggaatca aaaccgcact tcgtttcaca ccgaacgaca tccgggaatt
     6181 ctcccgtccg ccggtcatgc gcagcggtat ccactgggag catatgctgg aagttttcga
     6241 gaagtccggc gatgccggag cggatttcct ttccatcgaa tccaccggcg gaaaagaact
     6301 gtgcgacgaa gcgctgatca atgcggatct gcccggtgtt gttttcgcgc tgggcgtgct
     6361 gggatgccgt gatatggaat ttctctggaa acacatggtc gccgcctgcc ggaggaacgg
     6421 tgtgattccc gccggagaca gcgcctgcgg cttcggaaat accgccatgg tactggccga
     6481 caaaaaaatg attcccaagg tttttgccgc cgtggtgcgc gtcgccaccg tgtcgcgcac
     6541 tctggtcgcc gtcgagcagg gcgcagtcgg cccgtgcaaa gactgcgcct acgaaggcgt
     6601 ttacatcaaa gccatcaccg gaacgccgat tgcgctcgag ggcaaatccg ccgcctgtgc
     6661 tcacctgagt ccgatcggca atattgccgc cgccgtctgc gactgctggt ccaacgaatc
     6721 ggtgcagaac gtccagcttc tcagcggcaa ggcaccgatg gtctccgccg aacagctggc
     6781 ctatgactgc cgcctgatga atgtcgcagc gcgccgttcg ccggaagacg ctctgcggct
     6841 gcgcgactgg ctggtcgagt ccgacgcgcc gctcgatccg caggcgtatg tgctgcatcc
     6901 ggaagtcgtc ctgcgcattt ccagacgaat tgtacagggt gcaacggctt atgaacgcac
     6961 ccgcatcgcc gtgcaggccg ctctggaaga actgcgcaag ggtgctaatg aaaaactcta
     7021 ccgccttgac ccgcgcgaag aaagctggct cgatatgatg gaaatggcca ttgagaatga
     7081 cattccggaa accgaggcgg aaatgattgc gcagcaggct ccgctcgtcg acaaatccaa
     7141 gtgctcactg acagaatatg gaatcgaagc gtaaagaaaa aatcccgtgc cacttgatcg
     7201 ccggaccgct gggggttggc aagaccaccg cgatccggcg gtttgtcgcg caatcctccg
     7261 gcttcaccgc cgtcatcgtc aacgacttcg ggaagaccgg gtatgatgcc gcattcattg
     7321 ccgaggcggg cggggcggag cggttgcggg tcgaaaacat ccccggcgga tgcctttgct
     7381 gcagttcggt cgcgcagctg ctgccggcct tggaaatgct ttgcgcacgt ccggaggtgg
     7441 aacggatcat catcgaaccc agcggcattg cgctgattga tccgctgctg gaactgctgc
     7501 gctatgccgc gccgcgctgc ggcctggatg tcgcgccggt cattgtgctg ttcgatccgg
     7561 caaaaacccg gcccgccgca attaaactga ttccctactg gaaccggctg gccgactgcg
     7621 ccggaattat tgtcgccaac cgttgcgatc ttgcgccgac cgaagcggtt acacagtttt
     7681 ccgactgcat gaaacaatgg aatcctccca agctgaaggt gatcgaaacg tcgcacggcg
     7741 aacttccgcc ggagcttttc gaactgcgcg ggcacacggt tccggcggcg ggcaacgccc
     7801 atcctcacgc cgatctcccg ccggccggcg cattttgctc ggacggaacg ttccggctcg
     7861 ccgcactgct tgaactgctc gaagaactgg cgccgcagct cgaccgtttc aagggcgtat
     7921 tccggacgga ccaaggttgg cagcggctgg aaatcgccgc cggacacatc aactccaggc
     7981 cggcggtcga ggccgcgcaa acttctgcgg agtggatcgg cggcacggat cggctggcgg
     8041 aacggatccg tcactgtcgg atctgaccgg caaaatagtc acataaaacg tcctaaaccg
     8101 cgccatttag gcgaatatcc ggcaagatcg ttatataaat tgcatacatt tcctatcggg
     8161 ttccggcatc ttgccgtagg tttctgcgcc tacgaacaag gagtgttatg caaaaacagt
     8221 tttatctcga tctggctgac cgcggacttc gcctgcccat tggcacccac ctgattcttc
     8281 acgaaaaggc gaaccacgac accatcattc accacggcga ccagctcgga gccgtgatga
     8341 tcgaagcggc gcagtattac aagagtccgc tggcgattcc gctgatggat ctgacgctgg
     8401 aaaaagaggc cattctggcc attctcggcg tgccggccga agagcgcgaa aagtttcatt
     8461 tcgacaagct gcccgccgcg gatgccgttg aaaaaatcag cacggctccg ttcatgattc
     8521 cccgcatgga agccacctgt gaagcggtgc gctatgtcgc cgaacgtgaa cagcaggaaa
     8581 acattgtcgc gctgggaatg gggatcggtc cgttttcgct gatgaccaaa atgctgagcg
     8641 acccgattac gccggttttc atggccggca ccggaatgac cgctgaagac gatgacgatg
     8701 tggcgctggt ggaatgcgtg atggaacttt cgacgcagat catcctgcgt tatctgacgg
     8761 aacaggccaa agccggggcg aaagctatta tcctctgcga gccggccgcc aatctggttt
     8821 tcttttcgcc caatcagctg gcggaaggtt ccgatgtgtt cgagcggttc gtgatgcagc
     8881 cgaatctcaa aatcaaagcc aagctcgatg agctcggcgt ggatttaatc ttccacgact
     8941 gcggcgagct gaccgacggt atggttgaat ccttcaaccg gctgaatccg gtcatgctca
     9001 gcctgggctg ttcccgcacg ctctggaaag atgccgcgct gctctccaaa gacattgttc
     9061 tgtacggcaa tctgccgtct aaaaaattct actccgacga agccgtcagt gtggcggatg
     9121 tcgaacgcat gacccgcgag ctgatcacga atatgaaaac ggccgggcat ccgttcatta
     9181 tgggcaccga gtgcgatgtg ctgagtgtgc cgggtcacga agagagcatt aagcgcaagg
     9241 tggacgcgat gatgtcatgc agtgtataag ctgttgccat ggccgtctgc ttcaagtcaa
     9301 acaggcacag cgccgcagtg gaaaaccgct gcggtgttct gctgtcggat ctggccgcag
     9361 aagccgggtt gccgctcaac atgcagtgcg gcgggcaggg cgcctgcaaa ggctgtctcg
     9421 tcaagctggg gccgggccgc taccggatcg ctgaaaccga aacggttgtc gcggacgggc
     9481 agcgacttga ggctcgcgcc tgcaaaacgg tggtgctgga cgaacgggtg gaaatcgaaa
     9541 tacccgaacg ttcgctgctc aaaatcggcg aatgcgcggc ggcggatttt tatgccaccc
     9601 gcaaaaatct tttccatccg cacaaaaacg ccgccggaca gctcggcatc gctgtggata
     9661 tcggcacaac gaccgttgcg gcggttctcg ttgagctttc caccggaaaa attctcgcgc
     9721 gcgaaacggc ctataaccgg cagatcgaac tggcggacaa cgtcgcttcg cgcattgcgc
     9781 tctgtacgga tgacgccagc ctgaaaaagc tccagagcct gattattcaa ggaacgctgg
     9841 tgccgatgtt cgggaaactg gccgtacaga gttcccggtc gctggaagag gtcaccggta
     9901 ttgttttctc gggcaacacg gtgatgagcc atctggcgct cggactttct gcgctgagca
     9961 tcgggatgat tccgttcgag ccgctcacca aagtcttcag cgaacatgcc gcgccggaaa
    10021 tcggcctggc cggctgcccg aaggcccgcg tacggacggt gccggccatc gccggttatg
    10081 tcggcggcga cctggtttcc gacctctata tgtcagaacc tgatccggcg cgccttgagc
    10141 tgattgtcga catcggcacc aacggggaaa ttattttaaa cgatcgcgga cagctgatgg
    10201 ctacggcgac cgccgccggt ccggcgttcg aaggcgccgg acttctgcac ggatgccgcg
    10261 ccgccgccgg agtgatcgaa aaaattatct gccaccccga tgattcgatt gaatttgaaa
    10321 ccatcggcgg caagccggca accggactgt gcggcagcgc ggccgttgat tttattgcta
    10381 ccgcctggcg caccggtctg ctcaacagca tgggccggtt tgatctcgac cggttgcgtg
    10441 cggcgggccg cctgcatcaa cttgactgct gcggaaccaa cgtcaacacc tgcgtgatcg
    10501 taccccggga aaagtccgga acggatgaag ccatcctgat tacggaattt gatgtttcgc
    10561 agatcatgaa agccaaagcg gccgtttacg ccggtatccg cacgctgctg gaacgcggcg
    10621 gacacgctgt cggtgatgtc gaacggctgg tgctggccgg cgggttcgcc gcgcatctgc
    10681 ggattgaaaa tgccattacc atcggcctgc tgccggagat tccgctggaa aaatatgacg
    10741 tggtcggcaa cggatcgctg gccggcgcct atgcggcgct cggtgagggc ggagtctggg
    10801 acgaactgca ccggattgcg gcgctgccgg gctcttgcca tctggcagag accaaggagt
    10861 ttgaaaaccg gtttattgac gctttggcga ttcctaatct tgaccccgat gaatttccag
    10921 tgacaatgtc gtcactatga aaattaccga cgcagtgaaa aacggacgga tattggtttc
    10981 cgacggtgct tggggaacct tcctgcaaca gaaagggctg aagcccggcg aatgtccgga
    11041 actgtggaat ctggagcgac cggatgatgt gagggccatt gccgccagct acattgacgc
    11101 cggcgccgac atgattgaaa ccaacacgtt cggcggaaac cgttttaaac tggcgcatta
    11161 tggtctgacg gaacgcgtcg cggaaatcaa cgaagcaggc gcgcggcttt cgcgcgaagc
    11221 ggccggctcg gatcactggg tgatcgcgtc gatggggccg accggcgaaa ttctgattat
    11281 ggaggaagtc tccgaacagg agatatacga ggcgttcaaa gagcaggctg ttgcactgga
    11341 gcgcggcggg gccgatgcca tctgcattga aacgatgagc gcgctggacg aggcggaact
    11401 agccgtcagg gctgttaaag agaataccgg ctgcgaggtg atctgtacct tcacgttcga
    11461 gcgcacggtc aatggcgatt accgtacaat gatgggggtt tcacctgagc agtttgctgc
    11521 ggcgattctt gcggcgggag ctgatattct tggcgcgaat tgcgggaatg gcatggagcg
    11581 gatgatcgac attgtgcggg ctctgcgcgc cgcggcaccg gctgcgccga ttctggtaca
    11641 cgctaatgca ggactgccga aaaatgtgaa tggagtcgat acctttccgg agagtcccga
    11701 agaaatggcg acgcggggtc ctgcgctgat tgccgccggg gcaaacatca ttggcgggtg
    11761 ttgcggcacg acgccccggc acatcgctgc attaaaacag gccattcggt agaatgtctg
    11821 gggggcgggt tcccggcagg tgtttacgat gacgtgtcgg cgcgaggggg gactgttgaa
    11881 tcagtttgtc tcgctgacgc tcttctttga cggagcgctt ctcggggtac tcccaggtgc
    11941 tgtttgttca tgatctcatt cagctcagga agataatgcc ggcattcagt aggcgtcatg
    12001 cgcgcaaagt tccggaagct ttcggcaaaa tattggctgg tgctgaagcc gcaatccaat
    12061 gcgatttcgg tgatggattg gtcggaggtt ttgagccgtt cgcaggcctt ttcaagacgc
    12121 aggcgacgga ggtatttctt ccaatgacca tcgtttgctg cagcgaggtc aggaaccggt
    12181 ggattcgagc actggacggc gaagtatgag gctgttcgtc agggctgttc cggatgattt
    12241 ccgcaacctc gagtggcgtc gattcctgga gtttgaggat catggccgga atcaggacct
    12301 gactgagtag cgcttcccct gcggactgaa gcccggcgaa tgtccggctt tttgttcggc
    12361 tgctgaaaag gccgttatgt cggatattta ccaatgagga ttatcgaacg caaggtattg
    12421 tcccttctgg taaaacgggg gccgatttaa gtgaacggct ggtaaccgtt gattcataac
    12481 ccgtattgtt tacctctaaa agaaagaaga atgctgtgaa aaaaatcata tccttgatta
    12541 ccggagtcct gctggcggcc tcctggggac actccgcaga aaacgaatcc gtgcggcatc
    12601 aaatgctgac cgccatcgac caatccatcg aaaaaggcag cttcaagccg acctgggagt
    12661 cgctgagcaa gcacatcgat ccagaatggt ttcaggatgc caagttcggc atctatacgc
    12721 attggggccc ggtcacggtc ggatccaccg gcggcggctc ccagtggtac ggaaaggcca
    12781 tgtatgaacc cggaaacaaa accttcgact atcaccgcca gcatttcggc gatcagaaca
    12841 aggtcggctt caaagatctg gttcccaagc tgactgcgca gaagtttaat gccgacgagt
    12901 gggccgacct gttcgcccgt tccggtgcaa aattcgccgg gcctgtggcg gttcatcatg
    12961 acaattttat gatgtgggat tccaagttga cccgctggac cagcgttaat atgggccccc
    13021 atcgcgacat cacgggggag cttgagaaag cgattcgcgc ccgcggcatg aagttcatca
    13081 ccacgtttca ccacgggttt gcctggcgct atttcgagcc ggccttcaag tatgacgcgg
    13141 ccgatccgca atatgccgat ctctacaccg agatccatga gccgaatgcg ccgcccagca
    13201 aagcgtttca ggatcagtgg ctggccctgg tttatgaggt gctcggaaaa tatcagcccg
    13261 acctgatctg gttcgacttt gaattcttcc gggtcatcca gccggcctac cagcaaaagc
    13321 tctttgccat gacctacaac tgggcgaatg aaaacaaccg caccattggt gtctgccaaa
    13381 aaagcccgga gatccgcaac tataccggga tcttcgacat tgagcgcggg cgtgagaaca
    13441 gcatcaaacc ctatgtctgg ctgaccgact caatggtttc agactggttc taccgtgaat
    13501 cgtccgaggc caagtcagta cacaaactca tcagcatgct ggtcgatatt gtttcaaaaa
    13561 atggatgcct gctgctcgat gtcgggccga ccgtcgacgg aacgattccg gacaaagatc
    13621 gcgacgcact gctgggtatg ggagcctggc tcaagatcaa tggcgaagcc atctatgcaa
    13681 cccgcccgtg gaagatctac ggcgaaggtc cgacccgcct ggccaaatcc ggcaccttcg
    13741 gcgaagatgc cgagaagccc tatggcgcac aggatatccg ttttacccaa tcgaaagatg
    13801 gaaagacgct ctacgctacc gcgctcggcg tccccgaaaa tcggcagatc caggtcaaat
    13861 cgctgtcggc cgccgccgga aaaatttccc gtgtcgagct gttaggccat accggagcct
    13921 tggagtggaa acagactgct gaatcgctgg ttgtcacgct gccggcaacg ggaacctatc
    13981 aaaacgctat ctcgctcaag attcacgccg gcgagttaaa ccccgtttcc gtcatctacg
    14041 acacctcgat taaagccaat gaatccggca acatcgaact cagcgcaacc gctgccgaaa
    14101 tccacacggc cgataaacat acgccggagg cgaagaaacc tttgattggc agctggaccg
    14161 acccgaagga ctttgtttcc tgggagtgta ccatcgaata ccccggcacc tacgaagtcg
    14221 acgtgaccta ctcctgtgca acccagcccg gaagcgcctt ctatgtggag atggaccgcc
    14281 agaagctgga tgggaaaacg gcctccaccg gcatgttgac ctatcgcacc gatcatctcg
    14341 gcgtactgta tgttgagaag cccggtctcc acacaattaa cttcaaggcg aaagccgagc
    14401 cggaatggca tgggatcgga ctcagcacca tcgttctgaa gcgtattatg taaacgggtt
    14461 ccgggctggt ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttcaacag
    14581 ccggcgttgc ggttcactca gcgacccgcc ggcagcccgg tctgattgaa atattttttg
    14641 cggatgtaca gcgacacatt caccagcccg atcaataccg gcacttccac cagcggaccg
    14701 atgacggcgg cgaacgccgc accgtgattg atgccgaaga cgcccacggc gacggcaatc
    14761 gccagttcga agttgttgct ggccgccgtg aaagaaagcg tggccgattt ggcatagttg
    14821 gcaccggcct tggcgctcat ccagaaactc accagaaaca tcacgacgaa ataaatcagc
    14881 agcggcacgg caatcaaaac gacgtcgccg ggccgggcga tgatcttctg cccctgcatg
    14941 gaaaacatca ccacgatcgt gaagagcagg gcaaccagcg tgatcggcga aatcttcggg
    15001 ataaactggg cgtggtacca ctcccggccc tttgctctga ccaggaaaaa gcggctgagc
    15061 attccggcga tgaatggaat gccgagatag atcagcacgc tgactgcaat ctgccggatc
    15121 gtcacattca ccacggcgcc cgaaagtcca agcaggggcg gaagttttgt gacaaacaac
    15181 caggcgtaga ggctgtagaa gaacacctga aaaatcgaat taaaggccac cagcccggcg
    15241 cagtattccg cgtcgccttt cgccagatcg ttccacacga tcaccatcgc aatgcagcgc
    15301 gccagcccga tcataatcag cccgatcatg aactcggtct gattgtgcag aaatacaacc
    15361 gccagcgctg tcatcagcag cggcccgacc acccagtttt ggagcaggct gagtccgaga
    15421 atcttgcggt cgcggaacac gtcgcccagt tcctcgtact tcaccttcgc cagcggcgga
    15481 tacatcatca ggatcaggcc ggcggcaatc ggtatgctgg tttgcgttcc ttcgggccgc
    15541 aacgagctga tccagactcc aaatgaaggc acaaaatagc cgagcagtac gccgaggccc
    15601 atcgccagaa aaatccacag cgtcaaaaag cggtccagaa acgacagtcg tttggtaatg
    15661 ccctcgttca ttttttcaat ccttccggca gggtttcaat aaaggctttg atttcgtcgc
    15721 gcacgcggcg gtagcagtcc agctgagctt catcatccgc gccctgcgcc gccagttcgc
    15781 gggcgagttt cggcgggtca tcaaacccaa cgtggaggat cttcgcttgc ccggggaaaa
    15841 gcgggcaatg ctcgttggcg tggccgcaaa cggtgacgac aacatcgaac ggaatgtgca
    15901 gcaggtcggc gacgttttcg gatttgtggc cggaaatatc cacgccggct tccgccatca
    15961 cctggacggc cttcggattg aggccgtgcg tttcgatgcc ggccgagtag gcttcgatca
    16021 cgtcgctttt cagcgcgcgc gcccagcctt ccgccatttg gctccggcag gaatttccgg
    16081 tgcatagaaa aaggatcttc agttttttca tcggtgagtt ccttacgctg cggtgtagat
    16141 cagatagagg ccgcccagca gcacgagcag gccgcaaagt ttcctaagaa tcagcgcgcc
    16201 ggcggaatgt tcattccagt tcagatagtg ctgaacgact tcggtgaaag ttccggccag
    16261 tacgatgacg gcgcagtgac cgatgccgta gagcagcagc agactgcccg cataaaccgg
    16321 ccggccggcg ccggctttga aaacgactcc gagcagcggc gccataaaag cgaatgtgca
    16381 ggggcccagc gcgaggccga acaccagtcc gagaatcagc gcggccagcg caccgcgccg
    16441 cttcatgttg acctgaccgg ggcggctcgg caggggaatc acgccgatca gatgcaggcc
    16501 gacgacaaag aaaatcagcg cgatgaaata gttcgcgtac ggcccgagat cgcccagaat
    16561 ccgcccggcg gcggcggtca atccgccgat catggcgatc gtaatcagaa tgccggtggc
    16621 gaacagtaga gaaattccga aagcgcgccg ggtcgagatg cggccctgtt cgtcgatgaa
    16681 gccgacaatc agcggaatac tcgccagatg gcaggggctg aggagaatgc tcagaacgcc
    16741 ccagaccagc gcggcgctca gcgccatcag cggctcgccg tccagtgcgc gggtcagcgc
    16801 ggtaaacaat tcagtcacag ctgaactccc agctctttcc acttggcgag gatttcgtcc
    16861 ttggcaaaga acccttcgtg ccggaaaagc tctttgccgg cggcgtcata aaaaatctgg
    16921 gtcgggattg tgcggatgcc gtactgctgc ccggcctgcg ggttgtcccg gacgtcaata
    16981 aacacaacct ccagcttccc ggcgcaggtc gttttcagtt catccagaac cggcgtcatc
    17041 agtttacagg gaacacagct cccggagccc agatcaacga gacggggcag gggtttttgc
    17101 gccggagttt cggcagacac tacgggccgg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17221 nnnnnnnnnn ngccagcgcc ggagtgatca tgacgccgaa cttcatgatg tcactgagct
    17281 gcgtgacctt ctcgatctcg gcgtcgagcc ccagcccgtt cacggcctct tcggcatgct
    17341 gcgcgagttt tttacatttc gtacagcccg ttcccagaat ctgaatcttc ataaccagtc
    17401 tccttcgctt acatgatgag attaaataaa taaccgacga gcatgatgcc ggccgccacc
    17461 acggcaaaga aaaccgccag caggagcggc ttcagcacct tgcgcaggat gatcgcttcc
    17521 ggcagcgaga gcccgatgac ggccatcata aaggccagca cggtgccgag cgctgcgccc
    17581 ttgccgagca gcgcctgaac aattggaatg attccggcgg cattggaata catcggtacg
    17641 ccgagcagca cggccaccgg caccgaccac cacgttctgc ccatgatgga ggccataaag
    17701 ttttccggca cataaccgtg aatgccggcc cccacgccga taccgatcag cacatacagc
    17761 cagacccggc ccacaatatc tttcaccgcg tcgaggccga accggatacg cgccggccag
    17821 tccagttctt cgtccggcgc gccgtcgccg gtctgtgtct ggtaaaccca cgcttcgacg
    17881 tgcttctcca tccgcagccg accgatgacc catccggccg caatcgcgat aaccacgccc
    17941 gtcaccagat agatcgccgc gactttccag ccgaacagtc cgaacagcag caccagcgca
    18001 atctcattca ccatcggcgc cgaaatcaga aaggagagcg tcacgcccag cgggaccccg
    18061 gtcgtgacaa agccgatgaa cagcggcacg gccgagcagg agcagaacgg cgtgacaatt
    18121 cccagcagcg cggccagcac attgccggtg aactcgcgct taccggccag catccggcgc
    18181 gtccgctccg gagtgaaaaa cgagcgcaca atgccgacgc cgaacacgac cagcgtgagc
    18241 agcatcagca ccttcgggac ttcgaagata aaaaactcca ccgcggaggc cagatgcgag
    18301 ccggacttca ggccgagcag cgtatcggtg agccaggccg ccgccgccgc gagatggcga
    18361 tagaccagcc accagagcac gaggcccgcc gcgccgcgcc agatataggt attcgtcggc
    18421 ttactcatcc cgacctccgg gtttgcctgc gatgattttt tccagcgcac gccggtcggc
    18481 tttacgcgtt ccgtccgcat tcagctcggc ctccagccac tgccgcagcg gagcgggaaa
    18541 atccgcagcc agccggtagt ggacccagcg gccggcctta cggctctgca ccagcccggc
    18601 gcggatcagc agctccatat gccgcgatgc cgtggcaccg gtgacttgga gcagctcgat
    18661 aatctggcag gcgcagagtt cgtcgtgatg catcagcgcc gcgaccaccc gcaggcggtt
    18721 gcggtcggaa agggatttca gggcgctcag gggcttgttc atgccgtctt tatacttagc
    18781 cgtttggcta aacgtcaaac tgttaggctg taaattttac cggcaccgcc gatgtaaacc
    18841 ggtgttgaaa atgacagaat cggattgcgc tggcggcggg tcgcggccta gagtctccgc
    18901 tcttttttta ccccggatgt ctgctatgag ttttgaacag gaaatcgcca agcgccggac
    18961 gattgcgatt atttcgcacc cggacgccgg taaaacgacg ctgaccgaaa agctgctgct
    19021 ttacggcggc gcactccagc tggccggttc ggtgacggcg cgcaagagtc agcgggcgac
    19081 gacttccgac tggatggagc tggaacgcca gcgcggcatt tcagtcacgt ccaccgtgct
    19141 ccagtttgaa taccgcgggt gctgtgtgaa tctgctggat acacccggtc accgcgattt
    19201 ctccgaagac acctaccgcg ttctgatggc ggtggattcg gtggtgatgg tgattgatgc
    19261 ggcgaagggg attgaaagcc agacgcgcaa gctgtttgaa atctgccgga tgcgcggcat
    19321 tccgattttt acgtttatga acaagctcga ccggccgtcg cgcaacgcgc tgtcgctgat
    19381 ggatgagctg gagcagacgc tgcaactgcg tgtctgcgcg atgaactggc cgctgggcaa
    19441 cggttctgat tttcagggtg tctatgaccg gcaggcgaaa caggctcact tttttgaacg
    19501 caccgcgcac ggagctttcc gcgcgccggt ggcgctgcat tcggtgaccg attcgcaggt
    19561 gcgcgaccgg atgcctgcgg aagtttatca ggaactgatt aacgagctgg agatgctcga
    19621 tactgccggc gaggtgttcg acctgccgga agttcaggcg ggagacctca gtccggtatt
    19681 tttcggcagt gcgattaata atttcggggt tcagctgatg ctggacgcga ttgtggatta
    19741 tgcgcctgcg ccgcagccgc gcttttcggg cgatgtaaag gtcctgccgg agaatccgaa
    19801 gttttccggg tttattttta aaattcagtc aaacatggat ccgaaccacc gcgaccagat
    19861 tgcttttctg cgtatctgtt ccggctgttt cacccgcgat atgcgtgtga cccattcccg
    19921 caccggcgaa ctggtgcggc tttccagttc acaccgtgtt ttcgcccgcg accgcgaaac
    19981 ggtggatgtc gcttatcccg gcgatgtgat cggcctcgtc ggccactcgg agttcggtat
    20041 cggcgatacg ctgacggaag acccgtcgat tatttaccat gaaatgccgc agtttgcgcc
    20101 ggagtgtttc gcgatgctct ataatcctgt ttctgcgcaa tacaaacggt ttcaggccgg
    20161 agtaaagcac ctgcttcagg aaggcgcgtt gcagtctttc cattataaag atcctgcgca
    20221 gaatgtgccg ctgctgggcg cggtcggtgt attgcagttc gaggtgttca aataccggct
    20281 tgaaaatgaa tatggcgcga cggcacggct ggagccggcg ccgtataaat ttttccgctg
    20341 ggtggacgaa tccggcggtg agaagtcggt ggttacggaa tccatgctgc cgcccagctg
    20401 ccgcatggca ttcgattcgc tggaccggaa agtggttctg tttgactccg aatggattct
    20461 gaagtttttc tgcgaacgca atccggaggt gaagctgatc agtctgccgg tttccgcgaa
    20521 ccttccgtcc ggcggacgtt aatccggccg gttggagttt gccggctggc cgctggacag
    20581 cgccttggca acgacagcgg ccagttcatt gagcatgaaa ggtttcggga gacagtacac
    20641 cgcaccgagc tcttttgcac ggttcagata atctccggga taaaatttat ttccgccgcc
    20701 tgatatggcg atgattttta ctttgggaag tgtccgctgg acttcttcga tcaattcaag
    20761 cccgtccatg tccggcatga cgatgtcggt gataagcagt tcaaccggac ccagcttcag
    20821 tttttcgaga gcctctttac cgtttttggc ggtgagtaca tcgtatttaa gccggaccag
    20881 catttctttg atgaccatgg tgatcccttc ttcatcatca acgaccagta tgtgagccat
    20941 aatatgcctc ccgcatgaat tcttcagttg tacggcgctt cttcccaggt tttcttctga
    21001 atggccaatg tgttgccgta attaatcggg ttcggatttg cgaatacggt gggatcatag
    21061 gtgattgttg cgttcgatcc catataaaaa ccttcccatg caatgactgt tccgcgaatc
    21121 gtctgcgtac cgcttttgaa gatgattccg tacgattttc cgtcctgtgc cggactgcgg
    21181 taaagcccgt cggcatagag tatgccttta aagttatcga tgcctgcgga tatccatatg
    21241 ccgcccatcg agaggagact gacaaagctt ccgtagaaat tgccgccgga aatatcaatg
    21301 tcacgataag tgaatacaat gttgttgccg tacagtgccg acttatttcc cagcgtgaca
    21361 attccgccga cgatcaggtt tacgttggca ggaatatctg cggcaaccgt aagattgctg
    21421 gctacaacaa tggttccggg gccggtcacg tttttatcga tggttgcaga cccgttgtaa
    21481 taattggtgc ctccgcccag acttatggca ctgccggaag gataagcgcc ggcattggtt
    21541 gaggcggcat agcccagcat attggcatag gccgttaaac gggaggcttc aatccgcggc
    21601 cccggaatcg ggtcgggaag gtcgcccggc gttgcttcgc cggtatttcc ggtaatgccg
    21661 ccggtcgcgt caatgatgtc gttcagctca gttgatgccc cgctggcaat atgcagcacg
    21721 ccatcctgat aaatggttcc gtttacggtt acattggccg cgatcgtgct ttgactcccg
    21781 tccagtccca tgagcgcctg ctgcccgccg ggtccggcga ccatgcggag ccggacaacg
    21841 ccggtcgcag aaaccgcgtc gttcgagact ctgccggtcg aggtgatgat ataggtgtcg
    21901 agcagggcgt tgctgtacac tttgctgatc tcgacgtcat aacctccgaa gccgttcagc
    21961 agcgtttcgt cgttactgaa gctgtcagga agtatcgcgc ggtagtctct gctggccatg
    22021 acacggctca aggcccgttc cagtccggct tcggccagcc agtgggcctg cgccgaccgt
    22081 aactgctgaa cggtttcaat ctggccgaaa gcgcccagct ggagcagggc ggctaccatt
    22141 aatgtgaccg ccagcagaac taccataacg gcgacaagaa ccgctccgtt ttttttcgat
    22201 gaagcaaacg ttttcatgat cagttccttg tattaacaaa cacttcattg gtgatggcga
    22261 tattgaagtt tgtattggcc agttccagcg agagcagaac gccgtcgtcc tgtttcaccg
    22321 aggtgaagac ccgcacgtta tcaggaatga taatgctgct cccgctgccg tcactgtaga
    22381 gcaatgtttt tccgcccgtt ggctggttat agagtacggt tttgttggtg accgcgctcg
    22441 gcagggtcag cggatttccg gcggtcaggc cggtataatc ggcctcacgg acatctttga
    22501 ccattctgac gaatgctaaa gccgcatcgc gccgaagatc ggcgtaggca ttgttaattc
    22561 gccaggactg aaaggccatc atcagaatga gggcaaccat cagcatcagg attgaggctg
    22621 cggcgattgt caccatcagc tcgatcagtg taacgccttt ttgtctgtct gttttcatgc
    22681 gattacctct cactgcttgg aacttccagc gaacggtagg tgacaaactc tacggttttt
    22741 ccgtcgcggt aggtgacggt gttggagatg accagatagt ggtaggcatc cgtgccgtaa
    22801 tactcggtat tggttgtgat gccggttgtg atgtccgtgg tttcaatggt ccagcctgcc
    22861 gtttttgaaa taccctgcgg ggcagggctg gccggattgt acagccgccg ttcgatatcg
    22921 gatgttccgg catgtatggc ggactgctcg agatcgctgg tgacggccag gtagcgtgca
    22981 tatgaaaacg agatcagcgc agccagcgcc ataaccgcca gcacggccag ggcaaggatg
    23041 atttcgatca gggtcatgcc gtcccttttt tttctgcgga ataattctgt tctcatggat
    23101 ttttctccat cagcttgatt tcaatatccg ttccgcctgc gtcgcgcagc aggatgcttc
    23161 gcgtccgtat ttcctgtacc gtgcagtctc cgaaacggtc gcccgtctga tagaccctgc
    23221 cgctgatcat ggcgaccggt ttttcctgcg cccaggaaat gccctgaagt ttaacaggcc
    23281 ccgaaaattc agacggttga gccgcctgtt gcagtccggc ttcttttgct gcactgaccg
    23341 gcgccggatt ttttatttcc acaagtccgg tgccgttgtt gtgaaagctc tctttcactg
    23401 catcctgcgc aagccggagc gttttgacgg gaagctgata tttctgttcg aagtacccgt
    23461 tatttccggc gtacctgcga ctgttggttt cagaccgcca gaaaaagacc gcggaaagca
    23521 gaagtcccgt cagaagggaa gaaaggatga tcgcagttct ggacggagcc ttcattgtgc
    23581 ggctcctttc tgcgcaattt gaattacgct taactctgcc tcaagcgcaa atccgccgct
    23641 gtccgtaccg tcgggcatga tttgaacccg tttttcatag gcgaacggat cgcgcacatg
    23701 aacgttattc agaaaggcgt aaatcccgtt tccggatccg gtgcctttta tcagaatatt
    23761 gatcctgacg gttccttccg gagctttatc cggagggtcc tgagtgatgt tgatggccgt
    23821 gatgttgaaa tcaatatgtt cagccgccgc attcagcagg gcgatgtgcc ggtcttcaaa
    23881 ctgtgcggca tcggattcaa tccggtacgt tgcggcgaac agggcgaatt ccgaaagccc
    23941 cttgctcaat tccggaatca gctgctcaag cgctttgctt tgagcatagg tgctgcgtgc
    24001 atgttccaga atgctgcggg aaaccagacc ggcgaccgct gtcaaagcga gcggggcgat
    24061 cagccacaga gccagcacac tcggttttct taagaagcag gccgtatcca tctgtttact
    24121 ccttaaatga aactttgtaa ttcatgataa atacatcttc gtttccggac tttccccggc
    24181 tcgttgttgc gggcgtgata gacgcgaagc attgcgccag ttcggggttt ttctgccagg
    24241 tttgcaggaa cccggcaccg ggacttttcc gcccgttggt cggcatggcg atactgaatt
    24301 caaatgccgg acggctgtca ttcttctgga taaacgtaag ccggtgcagg gtgcttccat
    24361 cggtctgact gacgagaacc ttcagcaggg gcagtaccgt gtgaatgtca gccggtaaag
    24421 cctctttgat tgcagtcgcc tgccgggtgc gctccgcaac ctgtttttgc agacggtcgg
    24481 cgtattgcgc catcgaaccg gacccggtat ataaactgtg aaactgcccc tggatggcgc
    24541 ggttgcgatg aagatagttc aacgcctctt gaatatccga gcaggctttc cagcaggaga
    24601 atgtaagtat tcctgcggac accagcagat agatcagcat accgcgataa atcagccgcc
    24661 gctttgcgac gggcattatg agggtgcggg ccaaattgat ttcaaactgt ttcatcgcag
    24721 agtcctccca aagccagacc gattcctgtc gctacattaa aagtttcagg aatcgaaaga
    24781 ttcagacggt ccacggcgat ccggggatca gctcccggat tcagaatatt caccggaagc
    24841 gcggtgttct ttgccagcgt cctggaaaaa ttttctttat ccggaatccg tccgatgagc
    24901 acgatttttt ccatcggatg ctgattggtt ttgagatgat aatagagcag ggcgttttga
    24961 atattttcca tcaggtagcc gaggttttta tcccagctgt cgttggcgga aaaaagcgtg
    25021 cgaggataga ttccgctgtt tgacagcatg atgatatccg cggtccggtc tgccagatta
    25081 attaaacaga ccggcgacgt atcggtttcg tcgcggaggc gtgtataaaa gttataaaga
    25141 gccgaacagc cgatctccac atggattgaa tacaaaccgg cggcccgcat aagattaaga
    25201 tgccgggtta tcgttttccg gggaacggct gccagaaacc ctgataattc gacagatttc
    25261 cgggcggttt ccacaggatt tacatgccag tcaaaacaca gttcattggc gggcttctgc
    25321 aaagcctcct cggcttccag ccgcaaggtc tgagccagtt cgtcggtcgt aaggttttta
    25381 tagttaaaat aacggacaat cagcgatcgg ctgtgcaagc atgtacgaac cgtgcggccc
    25441 ggcagttttt cttttttcca gaacacccgg agccacttgg ccagttgctg atccgtgaac
    25501 tcgaccggga attctcccgc agccagacga tccatctgga agccgctgcc ggcattggaa
    25561 aaatgcgccg caacaacccg tccatagtta atatccagac cgatcacatc ttcttcgcat
    25621 ccgaacagcc gtttcagccg tgttttcata acgatctccg ctctctttag actctgcggc
    25681 cgcacagcga ccgcagagcc ggcggattta cgccgtgttc agtttactga atatccacct
    25741 gtcccgatcc ggttccgacc atcgtaccgc cccacgtgcc ggcctgattc aaggtcagcg
    25801 tttttccgct gagaccgccg gcgttggcag ccgctgtggc agtaatggtg taagcgtcat
    25861 aggttccgcc aatggtcaca ccgcttccga cgaagtattt agcgttaccg acttcattgc
    25921 gaacaatctg cggcagctgg gcaaatgacg gccaggttcc tttttcggca tagtaaacct
    25981 tcgccgctgt tgcgactgta ctgcagatgt tctgtccttc ggtggcaatc gcgcgttcgc
    26041 ggttatttgc catcagaggg atgatgatgg ctgccagaat ggcaataatg gcagcaacaa
    26101 ccattaactc gaccagggtg aaccctgatt ttttattctt tgttctcatg gctcctcttc
    26161 ctttcctgtt ttccggtttg tttccgtgaa cccgtccgtc cgggctgcgc tgcggctgtg
    26221 ctttttatcg aatatgtgta gatacggtga acaccggcag ataaatgctg agtaccagta
    26281 ccagcaccat cagcccgaaa acgcttataa tgaccggctc cagcagagcc gttgctgtaa
    26341 caatcgaggc ttcaacctga tcctcatagg catctgcaac ccggtccaga acgtcgggaa
    26401 gtctgccggc gctttcgccg acccgaagca tgcgaatcag caatgaaggg aatatgccgg
    26461 tttttttgaa accatccgca atgtttgatc ccgagatgat ttgcgcctgt ccctgaacaa
    26521 tcgcctgttc gattattaaa ttatcaccga tggcggaaac aattctcagg gtggtcgaga
    26581 tcggtacgcc gctcttcagc atgatggcgg tacaccggca gatgcgggcg agaatatagc
    26641 gtttcagaca gactccggta atcggcatct tcaacttggt tcggtccagc cacagccgga
    26701 ccgacggtat ccggcggcag aatataaaaa cgactgtgag cagcaccgcg aacgccaaaa
    26761 tgagcggaac gttctgcagg ataaagttat taagaccgaa cactccccgg gtcagcgctg
    26821 gcagcttggc gtgcagatcg ttaaagatat cctggaagcg cgggataatc gctaccacca
    26881 tgaccagaca gatgatgatg aaaaaagccg ccacaaagat cggataggcc atcaggcttt
    26941 taattttgcg gcgcagcttg tcgcttgatt cgatatatcc ggccaggcgg ttcagagttt
    27001 ctcccagcgt accggactgt tccgccgcct gaaccatgcc gataaacacc ggcgaaaaaa
    27061 ctttcggatg cgcggccacg gcgctcgaaa atggaattcc gtcgtacagt tttttaataa
    27121 tgttcgccag aaccgctttc agcgcaggct gatccatgtc ttcgtgaata ccttccaagg
    27181 cttcgcgcaa tggcaatccc gagtttacgg agatggccag ctggcggcag aacaccgcca
    27241 tatcagttaa atggatcttc ttcgatcccg gatttttttt cggcgcgagc tgcaagtctc
    27301 cggactgttc tttcgatctg cgccggaaga gttgtccgcc gacgtctttt gatttagccg
    27361 tttccgccga taccggcaga accggttcaa catcggtcag gctgacaacg gttaatccct
    27421 tctgccgcag ggtggtcaaa gcgctctgcc gcgactcggc ttcaatcatg tcacgaatca
    27481 gatcccccgc tgtatttttg gctgtgtaat tgaatttgtt catttgtcac ctctcttcaa
    27541 aactgtgttg cccgcaacac ttcgtccaat gtggtgattc cgtttttaac cttcatcatc
    27601 ccgttatggg ccagcgattc catgccgctt tcaagcgcgg ctttgcgcag gaccgtatcg
    27661 ctgcaatgtt caacgatcat ttcccgcagc gggtccgaaa cttccagaaa ctcgaaaatg
    27721 cccgtccggc ccagaaaacc ggtctgctgg cagtccgggc agccgctgcc ccggaagaac
    27781 gtccactttg cagcgccggg gcaaagctgt tcggccagtt gtatgtcttc gggcggcggc
    27841 agatattcct ctttgcaacg cgggcagatc atccggacca gccgctgagc gatgaccagc
    27901 ttcacggttg ctgaaatcag ataggcgggc accccgatgt ctttgagccg gctgaaggag
    27961 gagggcgcgt cgtttgtatg cagtgtgctc aaaacgagat ggccggtcag cgaagagcgg
    28021 gttgcaatct gcgcggtttc cagatcgcgg atttcaccga tcataatgat gtccggatcc
    28081 tgacgcagga tggaccgcag acagttcgca aaatcgagtc cgattttcgg atgaatatgc
    28141 gtctgattaa ttccgtcgat caggtattca atcggatcct cgacggtctg aatgttgact
    28201 tcggggtttt ttaaaaagct gagcgcgctg tagagcgtgg ttgtttttcc gcttccggtc
    28261 ggcccggtca gcagaataat cccgttcgga aggccgagga ttctgcggaa ctccttcagc
    28321 atcggaggat caaacccgac atctttcata tccaccagca gcgaggcccg gtccagaatc
    28381 cgcagtacaa ttttttcgcc gtgcgccacc ggcaggaccg atacgcgcac atcaatgacg
    28441 cggtcgtgaa ccttgaattt gaaccggccg tcctgcggca gacggtgttc tgcaatatcc
    28501 agttccgaca gaattttgat ccgggtggcg atggcggcat agatcgcccg cggtggaggc
    28561 gtgacttcgc gcagcacgcc gtcgacccgg aaccgcacgg cgacatattt ctctgcaggt
    28621 tcaaaatgaa tatcgctggc ccggtcgcgg attgcttcga gcagcagcag attgacgaac
    28681 cggatcaccg gtacgtcatt ggccttgtcc ttgagctgat ccagctcgac gttcacctga
    28741 tcgtcaattt caacttcaag atcgacatcc tccagtgaaa caatgtcctg aagtccttct
    28801 tcgatgtacg cttcttcttt gtaggagcgg gcaatcagcg ccaggatatc ctcttcgacg
    28861 ccgaccacct gatggatttc cagcttggtc tgcgaacgga cggtatccac ggcatccaga
    28921 tccagcggat tggccatgat gacttttagg gaactttcgt tttccttctc aaacggaatc
    28981 aacgtgtacc ggcgggcggt tgcttccgga accagccgaa tgacggaacg ctcaatgtga
    29041 tattcttcgg gaatttcaac aaacggtatg tgcagcagat tggaaagagc cagcgcaagc
    29101 tcccgcgaag aagtcagacc gcattccgcc gccagacgtt gcaacgcccc gtctttatcc
    29161 ttgtggtcga ggaagacctt ccggagctgc tcaattttnn nnnnnnnnnn nnnnnnnnnn
    29221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29281 nnnnnnnnnn nnnnnnnntg cggaaccaca ttgagctgaa tgccgatatt ttcatagtag
    29341 tccagcgtcg tggaggtgtt gttgttgccg ctgccgccgg acacattgga ttcaatgatc
    29401 gggaatttct gacctacaat aatggtcgct tcctgattat tcatggtcag gacacgcggt
    29461 gcggaaagca ctttcgtatc cttgtcttcc tgaatcatcc ggatcgccac atccagcgac
    29521 tgtccgttcc gggtgagaaa cgcattttga ttcagcgggg tcagagagtt cggccagaac
    29581 ccggtagatg tcgggtcaaa gtttcccgga gcgactgtcg aaagaatctg accgacagag
    29641 tattcgcttt ttgcataggt gaagtctaat cccagatctc tcagcagatt gttgttcact
    29701 tccatgaagc gggcttccac gacgatctgc tgcggtatga catccagctt gtcgagcatc
    29761 gccgatatgc gcgtcagact gctcggcaca tccgtgacga tcagtgtttt actttttccc
    29821 tgatccgccg catccgcttt acccatcgcg cgttcaagtt tgccgaggtt tccataggcg
    29881 gatcgctgac cgctgttacc gccgctgcgc gtggcaaact cccatccctt ttgaccgcga
    29941 actgtcatga cactcatcgt gccgcgcgga gaaagctgtt ttgcgagcat ctcctgaacg
    30001 tccgctgcat ccagaaaacg cagttcaaac accttggttt ccagcggttc gaccgctgcg
    30061 agttcagtca gcttttccag tttgctgatc acgatggtgt cgccgacttt ccggtatccg
    30121 aaaccgtacg gtttcagaat cacttccagt gcttcgtccc agcgcacacg gttcagatga
    30181 atattgacgc cttcacagac gacttccgga ccaacgacta cgttgcgtcc ggtctgaatc
    30241 gcaaatgcat tcagcacctg cagaatcgtg cctccatcaa cctgaatgga gacgtaatct
    30301 gttgcagcgg ccggcggggt caccgtgctg ccggcagcgg ttgtggtcga ctggccgaac
    30361 gcacccgcga ataggctgcc cgccagcatc agcgccagca gaaccggcgg cttgaattgt
    30421 attggtctgt tcattttctt ttcctttcat tgctgtgcgc ttcgatgatt cgaagcagct
    30481 ctttcggctt ggccgtcctg ctcagggcgg cctgtggacg gatcagatca agcgccagca
    30541 gctcctggag cgactcattc atggtaatca ttccctcgct gcgcccggcc tgaaccatgc
    30601 tgtagatctg ttcaactttc atttcccgga tcagattgcg gattccggaa ttaacgcgca
    30661 tcacttcgca ggccagcaca cgccgtgact ggtcctgcgt cataatcagc tgttgcgata
    30721 tgacaccgat taaaacctgc gagagctgga aaaagatctg ccgctgctgc tccgccggaa
    30781 agacatcgac aatccggctg atggcgtgaa ccgtgtcctg tgtatggagg gtacccagaa
    30841 tcagatgccc ggtttccgcg atggtcagtg ccagccggat tgtttcgagg ctgcgcagct
    30901 caccgaccat gatgacatct ggcgactggc ggaagatgct gtgcagcgca tcggtaaacg
    30961 attcggtatc gctgccgacc tgccgctgat cgacgatgca caggccgtgg ttgtgttcgt
    31021 attcaatcgg atcttcaatc gtaatgacgt gcagtttttt gtttcgattg atgtgatcga
    31081 ccatagccgc aagagtggtc gattttccgc tgcccgccgg gccggtcacc agaaacagcc
    31141 cggaggagtg gtttgcaaat tcctctacaa tccgcggcag acccagcgcg gagaatgagg
    31201 gaatctgatc cggaatgatc cgcgccgcga gtgcgacatg tccccgttga aaatatatat
    31261 taaagcggaa tttcgagatg ccggggaagt cgtacgagaa atcgatggac ttatagcttt
    31321 ccagaataag ccgctgccgg tcattcagaa gttcttcggc gagaatctta agatcgttcc
    31381 cggtcagcgg ctcttcagaa accttatgca gcagcccgtt gatccggatc tgcggcggtg
    31441 ctccggctga taagagcagg tcggaagctt tctgatccct gacttgttcg agaagcttcg
    31501 taatcgagtt gggttccata gcatcctccg cttacgagga cactgtgggt tgtgtgtatt
    31561 tttttcacaa ttaggggaaa gcggaagatc gggtcgggca aacagccgag aacggctcag
    31621 ttttttgctg atagtctttt gttaccgcgt tctcaaaccg acctctaccc agtgcaccgc
    31681 gtggtgcgcc agttcggcgg catcattcaa atcaaatttt tctttgatgt gcatccggta
    31741 ggtctcaacg gtcttgacac tcaggttcag agcttcggca atccgccggg tcgacatccc
    31801 ttttccgatc agctcgaaaa cctccagttc ccggtcgctg agtttttcaa tgctttgttt
    31861 aagcaccccg ctttttggaa tatgaccttc gctgattcgt ctgagcagcc ggccggtcat
    31921 cgccggactc agaaaaatct ccccgcccag cacctgatga atggctttca gcagcagatc
    31981 gggatcttcc tgtttcatca cataaccgtt tgcgccggca tttaaagccc gttcggcgta
    32041 aagctcctcg tcgtgcatcg agaatatcaa tatgacaata tgaggattga tctgccggat
    32101 tttttttgtc agttcaattc cgtcggatcc tttcaacgaa atgtccagca gcagcatgtc
    32161 aggatgagtt ttttcgacta aagaaatgca ttctccggca ttatcggttg ttccgcaaat
    32221 tgacagtctg gaatcgtccg ccaccaatcg gcacaacccg ttgcagacca ccgggtgatc
    32281 gtcgacaacg atgattctgg atgaccttgc tgcaggcata ctttatctcc tttttacgcc
    32341 ccgttaatcg gactgaacag gcagcgcacg gcgaccgtgt gatttgaata ctgttcgatg
    32401 tttaaacagc cgttcaatgc ctgcgcgcgc aaacgcatac cgcagaggcc gattccggac
    32461 gatttttcaa aatcgtccgg cacacagttg ccgttattct gaatgaccag ctcgccttga
    32521 tttgacccgg ttccctggca gagccggact tcgatttgat cggcctcgcc gtgtcggatc
    32581 gcattatgaa ttgattccgc cgcaatcaga tagagatgtt taaccatatc cggatctgta
    32641 attggaaggg cggtgagccg gttcgagaac acgcaacgga tttgatagca ctgctcaatc
    32701 cgctgacaaa ggtggcataa ggctgttgcc gcatcctgat cctggatttc agaaatggcc
    32761 atgcccgcca ccacttcacg gatccggttg accacctcat cggccagctc aacaattttg
    32821 tgtacttcct ccgcttcagg attaccgctt tcgcgcaacc gatgttcgag cgctttgctc
    32881 aacagaccga taccggtcat ctgctggcct acaccgtcgt gaagatcatg accgatgcgc
    32941 cgtttttcag tgtcccacaa ctcgagcatt tgacgctcaa cctcgtgaca gcacactccg
    33001 gaaaaaactg aacctgtctc acccatagtc tcttcctccg cgcgaaagca tagttattta
    33061 gtgccgattt gcaataacct attttccggc aattatttaa atgctgtata ccgccggaaa
    33121 ttaaagttag cctttccgca ttccggtgga gtcctccgga aaaagcggat cggcgggcaa
    33181 gattcggatg tttcggcttg accggttttg tttaaacgcg caattatgaa gcacttaatg
    33241 agatttttac gttttcggat ttaaaacagg aattgaaact atgctaaacc ggttgatggg
    33301 ccttttttcc agcgacattg gtatcgacct cggtactgcc aatacactgg tatatgtccg
    33361 tgatcgcggc attgttcttc gcgagccttc cgtagtcgcc gtgcaggccg gcaccaaccg
    33421 cgttcttgcc gtcggcgacg aagccaaacg catgctgggc cgtactccgg gcagcatcac
    33481 cgccatccgc ccgatgaagg gcggcgtaat tgccgatttt gaaattaccg aagcgatgct
    33541 gcggtatttc attcagaaag ttcataaccg ccgtcgaatg gtgcgtccgc gcgtggtcgt
    33601 ggccgttcca agcggtatta cagaagttga aaaacgcgct gtcaaggatt ctgcagtcca
    33661 tgccggcgca agggatgtgt ttttaataga agaaccgatg gctgctgcga tcggggtggg
    33721 actgccggtt caggaaccgg ccggcaatat gattgtcgat atcggtggtg gtacaacaga
    33781 ggtcgcattg atttctctgg ccggtattgt ctacagtcgc agcgtgcgta tcggggggga
    33841 cgacctggat gaagcgatca tccagtatct gaaacgggaa tataatctgc ttattggtga
    33901 acgaaccgca gaagatatta agatttcaat cggttcggct atgctgatag gcgaagagac
    33961 aactatggaa gtgaaaggcc gcgatcaggt cgccggcctt ccgaaaacgc tgattatcag
    34021 ctctgaagaa gtccgcggcg cgctcaaaga tcctgtttca ggcatcgttg atgctgtccg
    34081 tgtgacgttg gaccgctgtc cgccggaact ttcttccgac ctggtggacc gcgggatggt
    34141 gctggccggc gggggcgcat tactgcgcgg actcgacaag ctcattgctg ataatacagg
    34201 cctgccggtt catatcgcgg atgacccgct aagtgcggtc gccgaaggta ccggcgtggt
    34261 gctgcacgaa atcaacttcc ttcgtaaact ctctgaagcc cgcgcacgct aaatgcgcgg
    34321 actcccagtt tctctgtccc tccgggttct ctgaattaaa gaccggaacg ggtctatggt
    34381 tgaacggaaa agttttttaa aatgggcgat agcggtggca gcactgctgt tgctttttaa
    34441 tcttccgggc ggatgcaccg cccgcatcaa aggcattttc aaagatccgg tttctcctgt
    34501 agaaacattc tttataaaaa cagcccacag cctgaaagcg ggggccgata cggtacgggg
    34561 atttggcgga gtaatggaaa aaaacagccg cctctctgag gaagtaatct atctgcaggc
    34621 caaactggtc gggcttgaaa atctggaaca ggaaaaccgc catctgcagg accagctcga
    34681 tttctataaa cgccagaaac acacgctgat tccctgtcag gtgacagccc gtactatcag
    34741 cggatggtgg cagagcatgc gtctggataa aggccgcacc cagggcgtcg aggccgggcg
    34801 ggccgttatt tctcccgacg gtctggtcgg acgaaccgat gaagtttcgg cgtataccgc
    34861 tgaagtgctt ttgatttcag atccggcctg caaagtctca gcccgtgtaa gccgcaccgg
    34921 ttcattcggc ctggtaacag ggcttggcgt aaacgctcag ggatatccgg ttgcccgcat
    34981 gcagttcatt cacaaagatt ctccggtgcg ggccggcgat gccgtagtga catccggtct
    35041 cggcggggtt ttcccgaaag acattctgat cgggtacatc gaaaccgtcg gcatggaaga
    35101 agcgggactt tatcagtacg cagacattat tccgaaggcg gtaaccgaac tgatggatgt
    35161 ggtattcgtt accggcgaag aagagaacgc ggaggctgag gaatgagccg gcgcttcatc
    35221 actgtactgc tggcggccgg cacggttctt caaacggtgc ttccgtcctg gaatgcattc
    35281 ggcgcgctgg aacttccgct gctgaccgga ctgatcattt tcattgctct gcacacagat
    35341 cgtgcaagga tgttctatgc cgcagtgctg gcgggattgc tgcatgactc cttttgtccg
    35401 gctccgctgg gtctgtcgat tccgtttttt gtcattctgg ctgccggggt caactggatc
    35461 cgcgatgagg tattcggtga tcagccggca acctatgctg tgcttggtgc agtcgccgcc
    35521 gtttttgaac ttttgtacta tgcatctatt ttcaaattga gcggacttcg gccgatgtcc
    35581 ggaggattac tggcgcttcg gctggccggc ggtttgatgg ccggcacggt agtggttccg
    35641 ctggtggcgc tgacggttga gcagttggat cggttaatcc cgcgtaaccg gaggcggtac
    35701 atatgatgcg cgtccgacgg actgaccgcc actatatacc gcgcgtttac ctgctgacgg
    35761 gcttactggt atgtgccttt ctgctgcttg gagtttcgct ctggcggctg caggtagcga
    35821 aggtttcgca attcgaaagc cgccagcaga tccagagtct ccgccgtgtg cggctgccgg
    35881 gcatccgcgg aaaaatctac gaccgcaacg gtctgtgtct ggcggataac cgtccggttt
    35941 attccattgc tctgtttctc gaagacatgc gccgtcccgg cccctggtcg aaaacgatcg
    36001 accgcgcgat ggacgtcgtc gggcaggtga gtcagataac cggactcaag ccggtgatgg
    36061 atcgcgacgg catctggaag cacatacggt tgcgcctgcc gctgccgctt gtgctttgga
    36121 acgacatcgg tccgcagtcg atggcccggc tggcggaact gggttgcgat attccgggtg
    36181 tggatattta tacacaggca acccgtatct atccgtacag tccctacacc tcacatctgc
    36241 tcggttatgt cgggcgcgcc gacattgacg ccatgagcga agaggagagc tattcgtatt
    36301 acctgccgga aatgaccggc cgggccgggc ttgaaaaact tttcgacccg ttccttcgcg
    36361 gcgaagcggg cggtaagctg gtgcagattg atgtgtccgg ctaccgtcat aatgaactgg
    36421 cgcaccgtac gccgaagccc ggcggcgatc ttcgcctgac gctcgacatg gaaattcagc
    36481 ttttggcgca taaagcactg ggaacacatc gcggtgcggc ggttgtgctc gatcccaaca
    36541 acggtgacgt gctggcgatg ctcagtacgc ccggttttga tgccaaccgg tttgttcctt
    36601 atatcaacac ggcggattgg aaagcgctga gtgaagatcc aggcaaaccg ctgctgaacc
    36661 gtgccgttgc cggcgtttat ccgcccggaa gtatttttaa accattggtc gcgctgacgg
    36721 cagctacgct taacccggcg gccataaatg tagtttatga cagcccgtcg gttttccgta
    36781 tcggcagccg tgtcgtgcat acgaccggtc acggtgaagt cgatatgcgg caggccatga
    36841 agttttcggc gaatgtctat ttctttaaaa ccgcgctggc ctgcggtccg gaacccatca
    36901 ttcaacaggc tctggcggcc gggctcggac agaaaaccgg cgtcgaagtc gactttgaaa
    36961 gcagcggtac cataccggat gaaaagtggc agaaagataa ccggacaggc tggtcggacg
    37021 gtgatacctg caatctggcc atcgggcagg ggtatgtgac agcgactccg attcaaatgg
    37081 cgatgttgac ggcgactatt gcaaacggcg gttatctata tcgtccgcgg atggttcagg
    37141 catatcgtca gccgggatcc gaagattatg ttctgaatcc caaccatcgg attggaaaaa
    37201 tgaactggtc cgcggcagcg ctgcgcgccg tacgtaacgg tatgcgggat gtaattatgg
    37261 aaaaggacgg caccggtcac cgcgcagccg tggaaggatt tgagttcgca ggtaaaaccg
    37321 gtacagctga atacggcctt aaagaagaag gtgcgaagca tacctggatg accgcgtttg
    37381 caccgcttga taatcctcaa tacgccgtag ccatactcat cgaagacggc gataccggcg
    37441 gactgaccgt cggaccctgc ctgaaagttc ttatggaagg cctctatgcg aaaatgaagc
    37501 gggaagggcg gctgcagtca tgatcggtcg tcttcgccat tttgactggg tgatgttcgg
    37561 tgccattgcg gcgctgctca tactcagctg tctgttcatt tacagtgccg actgccgtaa
    37621 caccgannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngctg gtgctggtgc
    37741 tgtttatcgg catgtcggtt tatggtgcac accgctggtt cagtttcgga agcgttacgg
    37801 ttcagccttc agaattcgcg aagctggctg tcattttcac gctggcagcc tggctgggag
    37861 acccggcaac tgatattgaa cggtttaaaa cgtttatttt ggcgtttctg ctgaccggta
    37921 tcccgtttct gctgattgcc gccgaaccgg atttaggcag tgccatgata ctggttccga
    37981 ttgttatggt gcttctcttt tgcgcgggag ttccgctgcg tccgctgctg tcccttgtgg
    38041 gaataggtgt ggcagcggtt cttgtgctgg tcgtctggct gctgtttttt cccgacagct
    38101 gcccgctcga ggactaccag aaaaaccgta ttttggttta tttgaatatc aatcaggatc
    38161 cgctgggatc cggctggaat aaactccagt cgcagattgc agtcggttca ggaggatggt
    38221 ttggaaaagg ttatttgaaa ggaactcaga atattctcgg cttcctgccg agaacggtgg
    38281 ccccgactga cttcatctat tcagtcgtgg ctgaagaaac aggatttgcc ggcagctcgc
    38341 tgctgctggc tttatataca atggtggtag tgcgctgcat gcacgccgca atcgttgccc
    38401 gggacatgtt cgggcgattg ctgtcggcag gtatcggcgt gcttattttt tcacatgttt
    38461 ttgtaaatat cgcgatgacc ataggcctca tgccgattac cggagtaccg ctgccgctga
    38521 tgagttacgg cggaactttc atggtgagta tcatgacagc gctcggactg gttcaaagtg
    38581 tttgcctgcg tcgtcgtatt taaaaagaaa ggagcatcca tgctcagtat aagtgcaatt
    38641 aaagaaaaac tgttggccgg aagccggaag aagaaagaga tcctgattaa cattgagccg
    38701 ctcgaaaccc gcgtggccgt gttggatgaa cggaaactcg ataactttca tattgaacgg
    38761 tctgaagaaa gccggctggt tggcagcatt tttaaaggaa aaattcagaa cctcgaagac
    38821 gggttgcagg ccgcattcgt cgatatcggc atgcagaaga atgccttcat tcactattgg
    38881 gatatgatcc cggaagatgc cgcccggctg gaagagcagg aggggagtcg caacagcgcc
    38941 cgccggcgcc gcaaattttc acccggcgaa atgtccaaaa aatttccgat cgggtcagaa
    39001 atcgtcgtac aggtcaccaa agatgccatc gggacgaaag gtccgcgcgt caccgccaat
    39061 ctgagcgttc cgggccgtta tctggtaatg atgcccggca cagggctcaa gggcatctcc
    39121 aaaaaaatcg gcgacgcaaa agaacgcgtc cggctcaaaa aaatactggc gcgcctgccg
    39181 attcccgaca ccatcggtat cattgtgcga accgccggat ccggaacgcg aaaaacaagc
    39241 ttcgtccgcg atgcccgcac cttgatcgaa atctggaaag aaatcgacag cggcatgcgc
    39301 gaaaagcccg ccccgtgtgt tctctataaa gaacccaact tggccgaacg cgtcgtgcgc
    39361 gattatctga cggaagacat cgaccgcgtc tatgtcgatg accgcgaagc gtacgaaatg
    39421 atccggaaaa tgatcgccaa atattcccgc cgcgcccgca actgggttca gctctacgcc
    39481 ggtgaagaac ccatttttga ttacttcgaa gttgaaaaac agatcgaatc ggtctttcgc
    39541 cgcaaagtat ggctgaaatc cggagcctat ttgattttcg acgaaaccga agcgctggtg
    39601 gccgtcgacg tcaacacagg acggcacaaa ggcggaaaat ccgccgaaga atccatcctg
    39661 tccgtcaata tggaagcggc cgaagaagtc gcccggcagc tccgcctgcg gaacgtcggc
    39721 ggtctggtcg ttatcgactt catcgacatg aagagcaagc gcgaccagaa cctggtctac
    39781 aaaaccatga aagatgcctt gcgcgaagac cgtgcccgga ccaacgtcct gccgatctcg
    39841 cagcttggac tgctggaaat gacccgccag cgcttcgaag aaagcattta taccaccacg
    39901 tatgttgaat gcgattattg cggcggacgc ggtcgtgtaa aatcagcgct caccatgagc
    39961 gttgagctcc agcgccgtct ctccgctgcc ttgcgaaaag accggaaggg gatccattcc
    40021 atgaaaatca ccgtcaaccc gactgtcctc aaccgcctcc gccacgagga tgagcagaca
    40081 ctgatcgaca tggagaaaaa gttccaggga cacctgactt ttgtttccga cccgcacatg
    40141 cacatggaag catttaccat taccaatgag cagactcatc aggttgtcta ctccagtatc
    40201 gaaattgaca aatagaacac ctgtcatttt cagcattaaa agaagggcgc tctcatgggc
    40261 gctctttttt tatgattcaa acgggatgtt aatcaatgcg gctgcgctga taattttatt
    40321 tgcgcagatt gctaaaaaca aaactgaagc gctcgtgcct taatcgcgat tcgatggggt
    40381 tctggctgtg aacaaaatgc ggtcattcag ccgataagtg cgtccgctca tttacgcgtt
    40441 aaaatctctt ggtaccaatt cgtatgtggc gttgttgata actggctact aggagccact
    40501 tatcgacagg aaaccatcta aacacaacat attgtgcatc ctacaggtac atattggaca
    40561 taacatatat tatgcgacgt aatataataa agtgaattag gcgctgaatc gtgcagaaaa
    40621 ccaaatgaaa ctcgatcaca ttttcgatac gcgctagtat cggcttgagt catgctttaa
    40681 tggctgccgt ctgcaaatcc ggcgcgatca aacggccagc agacaaagaa tgccgggccg
    40741 a
//
LOCUS       DAOPZO010000002        40729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_839_length_40729_cov_10.636426, whole genome shotgun sequence.
ACCESSION   DAOPZO010000002 DAOPZO010000000
VERSION     DAOPZO010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 40729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40729
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_839_length_40729_cov_10.636426"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1579)
                     /gene="ilvD"
                     /locus_tag="PKI68_00200"
     CDS             complement(<1..1579)
                     /gene="ilvD"
                     /locus_tag="PKI68_00200"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNX52143.1"
                     /translation="MNSDRVKKGMERAPHRSLMHATGLTDEDIKKPFIAVCNSFNEVI
                     PGHVHLNQVGRLICEAIREAGGTPREFNVIGVCDGIAMGHEGMKFSLPSRELIADCVE
                     TMVEAHAFDAMICIPNCDKIVPGMLMGAVRCNIPTVFASGGPMAAGRLADGRVVDLIS
                     VFEGVAQCKAGKINEAELRQLECAACPGAGSCSGMFTANSMNCLCEAIGMALPGNGTL
                     LAIDPARRELWKEAARRAVEMAKANGPKPRDIITLESVDNAFILDMAMGGSTNTVLHT
                     LAIANEAGISYDLDRINRLSKTTPNICKVSPSSSWHIEDVQAAGGISAILGEISKIKG
                     LLNLDRPTVTGKTLGENIAGAVSKNEKCIHKVENAYSKEGGLAILRGNIAAEGAVVKS
                     AGVDPKMLVHKGPAVIFESQEEACAGILAGQVKAGDVVVIRYEGPKGGPGMQEMLAPT
                     SYIMGQGLGDKVALITDGRFSGGTHGACIGHISPEAAEGGPIGLLKNGDLIEIDIPNR
                     SLKADVSDAEFAERKKSW"
     gene            1685..3448
                     /locus_tag="PKI68_00205"
     CDS             1685..3448
                     /locus_tag="PKI68_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX52144.1"
                     /translation="MKTIFSATAALLAAGILLADEPATNIPPVVVTAAAQTAQNAAIH
                     EVLRAEPGVVLNSQGGSQNDLSVRGSSFSGAGLALGGMTLRNPQTEHFNAELPVPAAI
                     LSRPKVMTGLDNQGGHLTGTVGFDLLPIKGKKQVEAGFGSDHRDWQSLLVQQMLTDNL
                     GIGVFGGRESAGAVDYKDNDYDREYTGGHMQYREDDTQVDTLVAHQQKEFGARGYYGV
                     TDTIPANEKTEDTLAYLAARKGDLNADYLRGGVSWHEFHDDYELPTYLYRNQHRSQVS
                     SAFFDGRTLEVNGWALGWRADADQERIDSDGSGGLGHHSRSHGGVSLLPQWQGDRLKV
                     TAGARSEFFTGEQPYYLPQAGAEYMLSDTLTTFASYTETVRQPSYTELNYNSPFSLGN
                     TGLQPQTEKQTEVGFKGVPSEYTDWKVATFYRHSENTVDWMKTTSGSRWNAVDIGDLD
                     VYGTEARLGWYPVQTVEMQFAYTWIYKNKTAEDFDSYASRYALDYPEHLAQASLLWRP
                     VKPVEIGTVQSLRWQTDNEVRRNGDFGADSSFVVRFTPPKADYATLSFLLNNAWNDDF
                     QTFPGQRPPERFAGVSLTLTW"
     gene            3632..4090
                     /locus_tag="PKI68_00210"
     CDS             3632..4090
                     /locus_tag="PKI68_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNX52145.1"
                     /translation="MNWPTKRNDSYPVAWNTGLLHRSIDDLLADFFGGSAMEQAGLIT
                     PRFEVSETDDAVIVEAELPGMDEKDIELTLQDSILTIKGEKKKEEETKKKNCYISERS
                     YGRFQRSLQLGSGIDEDKVSAAFKKGVLTVTIPKSEPEKSKTRTINIKAE"
     gene            complement(4141..4216)
                     /locus_tag="PKI68_00215"
     tRNA            complement(4141..4216)
                     /locus_tag="PKI68_00215"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4181..4183),aa:Phe,seq:gaa)
     gene            4281..5258
                     /locus_tag="PKI68_00220"
     CDS             4281..5258
                     /locus_tag="PKI68_00220"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNX52146.1"
                     /translation="MNLFQPALLSTLPPMYVLDSLLPVFLIIALGLLLRRTGFFSETL
                     ATGLNRLTYWVALPALLLDKVTNATFDSGDVARMSLILVLATIGSALVAGLAAFLLRL
                     NRRSSGAFIQGAARSNNAFIGLPVILYAMSSLAPGVEALATVALAPAIVFYNILSVSV
                     LLMHSDRKTANEHQTGVLFAKQLLCNPLLLSCAAGLIFNCCGVHFPLALGRSLSVLGD
                     SSLALALLSIGSALTFKGAAKGLLHSTSAAVIKVFIQPLIGLGLAMLWNLPPIERQML
                     LIYLACPTAVVSYVLADIFDSDRELAAHIIVISTLLSTVSLSLIVAFGG"
     gene            5262..6449
                     /locus_tag="PKI68_00225"
     CDS             5262..6449
                     /locus_tag="PKI68_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNX52147.1"
                     /translation="MTVPRRRFGKTELQMPVITCGGMRYQQSWQDDDAARQSVTEENQ
                     KNVEATVRYALDLGINHIETARGYGTSEYQLGKFLPALPRDQMIVQTKVGPKDTPAEF
                     LESFDTSMAALKLDYVDLLAIHGINNEETFQQALKSMPALEQLKKEGRCRHIGFSTHG
                     TPDIVVKAIETGLFEYVNLHWYFVYHPVTWASVEAATKLDMGVFVISPVDKGGKLYEP
                     PAKLIDLCQPITPMAFNDLYCLRRPEIHTLSIGASRPSDFVEHVTAAGNMDKKFPIVE
                     KIEVRIFKAMEKELGADWVHHWHEGLPRWQDTPGEINIPEILRLWTYAKGLDMVAFGK
                     MRYNLMGNAGHWFGGMQAAEFKDEALKPLLEKSRFAGRIPEILREAHAMLHDEPKKRL
                     SES"
     gene            complement(6450..7673)
                     /locus_tag="PKI68_00230"
     CDS             complement(6450..7673)
                     /locus_tag="PKI68_00230"
                     /inference="COORDINATES: protein motif:HMM:NF015532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family outer membrane porin"
                     /protein_id="HNX52148.1"
                     /translation="MMKRTLLSVLLAALCTGASAESLLKPVNDLGYGTWNARLQTLSM
                     YRDVEGAKVDTAYSTTAGLLIGYISPELAGFSAGATWIYVEPVDATRDSNNGKTLLSN
                     GRVNILNEAWVKYRFESLGLTNTYIKAGRQVMNGELFRSDEIRQKPRSIEAVQLTTKD
                     IPNLSLTAGHAFRLSNVLDNEDGWRFNDFDEVLAPQGNGCGSPGISWGEAVFTGVTNL
                     EVALYDAYVHDIANVTGGRTRYALTDSTALLGYFQHADSVQTADKIAPFNSDMFSLAF
                     EQKAGGVTFEPGWFSVHGDDMIFHETTTGLNHPLGAPLLIYSGPFNGGSDSAYLKATS
                     KIGKTALYTLYSYTWQDHSRHAFDGQEINFFVKHPVTDSLSVAVKVGAGYREGCRGTE
                     DTTATDARLFVTYNF"
     gene            7828..8142
                     /locus_tag="PKI68_00235"
     CDS             7828..8142
                     /locus_tag="PKI68_00235"
                     /inference="COORDINATES: protein motif:HMM:NF018563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HNX52149.1"
                     /translation="MAEFKNVTVVKKANIYFDGKVTSRTVLFADGTKKTLGIMMPGDY
                     EFGTEAAEIMEMLGGQMDVLLPGETGWKTFKAGESYQVPANSRFKLKVPVVADYCCSY
                     LK"
     gene            complement(8145..10184)
                     /locus_tag="PKI68_00240"
     CDS             complement(8145..10184)
                     /locus_tag="PKI68_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52150.1"
                     /translation="MKKTIFAVGLGILLGTGCSHRAPPENPASFNFQLVSPEHVHMQM
                     LLANAMGYVNPSNGVIDAASGYPVEGWNHEPENGLYLRSFTQLTAIGEWVELLANIAA
                     GYADNPYISREEAFSNLSLTVSSLLADQSDPALSAKGLLSNFLGFENGKRIGPLAEEV
                     NRQAFVDEFGAEQADAIWQALAAHKWILPQQDGSFAKIPRKGEYGEKFFTGELEPFAA
                     DELRARIMALLDRRTVQIIFGDNANLTASAAKAFGALMHSEIKDFPEAVKLRGQLERF
                     IDRQAEGYRYLYGEESGTFAFGWNATMDRFTGWADGNGKWITGRMNYFVNEFRGPLCF
                     VVQRFDLPADAVKNSGMKIKPYRMADGRDLYTLATWHGSSFQSLGLSLFMQELDAQGW
                     RENLENSVDIELDFAIRHKLPGFLSEAYSGNGVEYTGDIGIPDVAVTDQARITNSPSL
                     YTLGVAYEVAPDKVEAFLASNWKTIRRLFTDHGPWEGFNTAKKEVIRFQTTAHTLALV
                     LGGIGSAGENMQRYLTWRGVTSLSKVQGGESSAVDLLSGGAKWITWSPSGDGLEGVHW
                     MNGFRVRGDAVQRGAVTIKLPQSVSLSNGALLIRYRAAAPVKNAVLTLKGAPIAFENG
                     IYARFDPDGSEKGIRIPMPATPGLEGVREIVLTFGDGTAAPVDLTISGFEFVPAR"
     gene            complement(10240..16239)
                     /locus_tag="PKI68_00245"
     CDS             complement(10240..16239)
                     /locus_tag="PKI68_00245"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52151.1"
                     /translation="MAKSFARVGILLAVLLQFASVPAAPVGFIDARQQGSFNIGAAQA
                     VISREFEEAPINRDLLIINYSIPAGTAAGVWAKEYPAALAAGSVDVVELGIYIPEGQL
                     SDVALAVEIKGSTGTQRFPVRPGPGWNTTRELIDWNKIGTLNETVLLIQRTGGADPAA
                     GRIRLDMTFQKLPLWENLRATLWGRLGGVTVFSLLLSLLVAVIRRKKRIPVRGFIRDL
                     MSGVATVLIISSVLGVYALGKMNPLDSGWFCFYVSAASVVISLLLKITLTGRPVFAGE
                     TLRSALFPGLLAAAAGKEVLWQAPDSLTGFFQLSGFGAALFVLIYHVANVCRLNLRRK
                     HLGLIGGTVITAAPFIFGLLLALQSFQYFAVQVLLIFCVGEFIAQASSMINREKLLFS
                     LKVHRALFVLSILVTAAPYIADLGSGPVAAALPAVFRPFAAVIATMLSQGPLWAMVYL
                     LTGMVLGAMHGSAPSGAVAQDDAVRGLKKGMVFSGVLMGLLQLFHALVSAAWIQNFYA
                     AAPALLLTVAGVAVFPLFKTVIETFDGSQSFFGRAACAYKDPVLYLRGLVLGLVFAAG
                     MSMNFAGLPTAERIGFGFAAGAAAFGGVSILRDLLFGLNGIGGVKSWRYYFVESGLGA
                     FIGAGIGFYLDAAQLPIIGTKFELYNSFGLNPADIVARCQQLRTTAPNEFTALISRWG
                     HIKLLAASGGAKILFNEALMGVIGWGIAAPLFAINKAFLSALLNRDISSIRRIATRGG
                     VTELADGTVYVLRWGLWMAPLIFTFLRPMAEAAWYNQDGLIRTFFAIFNSLTRDANGF
                     TVWSLTVFSWVIGYAWFQILIWIDHMGLRVATLVNLSFLGVDRLDERCAKFVGPEATA
                     RFIPEGVKRFTTWAPLLIPFYLPKGEQWAQVFSMGTAIQARQAGYVLGPVPLLLIAVA
                     AAAVVAALFTLRRKTRERHAAQTGQTFGLINNKYEVELKQSGEINSFMTSNGFGISRC
                     AFEKVDPSGRILFVVEAGEDGAANKAWPVLGNFPAELFTPSVYECDSDRLKIINISNG
                     VKVTVTIRLPGRNDGAELWNIELEDLSGKARNLKVVPYLEWMLNRPDADRNHTQYNRL
                     YPEMSYNTELNAMVAFHRSTKHYGILASAKQPAGILSGRVDFIGRAGNIWAPRVLETL
                     AFREALNMDACPAFDPIGALLLDAPVPANGKRSHKILIGCCEHKARIAQMIYKHLDLE
                     VDSSLHGIKQPERAQKIGHGEIPPGTPERYTEYLDDGRTLRVLTPFTPRPFDHEMANA
                     LGHIVAVTNRGLHSSASGNAQQNRLTPDWADLTGNQVPSEAIYLYDEKTGDWFSPCYD
                     PVKDKTAEHDVFFSLDGSVVFKMKKGGLETALSVHVPPDEPAGVYILKIRNTGIATRR
                     LRIAPYFQIALAHSPEQAGALFISRDAVSGALYFENPRNRFRAGSCFAAMSAPVEQFA
                     TRRDRFFGDGRSFSRPVMVETGAPAPAAGDEFPVAALLTTVEIPAGGEQTISIVLGEA
                     DSRKQAEVCVKKFQSIENAERSLQTTREWWMALQATLKIETSDPEFDAYVKWMKYQTL
                     SERIWARKGFYQASGAFGFRDQLQDTVNMIWVDPSLARAQLILHAAQQFPEGDTAHWF
                     FLQQDGRTGFLSRSYASDNLLWLGWGVGEYVRMTGDKTLLDEKVSYLKAETPLAPLPA
                     GKHGMGFFPLRSPRSDSVYKHVMRAVDLVLTKRMGSHGLPLMGTGDWNDGLDEIGSKG
                     RGESVWMAFFLTCVLKNLLPVIGERCGKKRRHYYEQQLRILGRNIEKVWRGDRYLRAV
                     HDDGTEIGIAGAGYWETDALGAAWAVFAGVNPQRSRIALDTALKILEGEKTISLGYPP
                     LRENTKPYLGRSSTYPEGVRENGMYSHGVQWLTRACRLLAERFAAQGDPVTAQYYRDA
                     AARLWYKISAIPHTHGAEIENYGGQPNQQCADYLTVHDPGRMIWSGYTGAAGWMLRQA
                     CESVIGATLADNKVILPSDLGLPRGDLIVKSIRRDLARSPF"
     gene            complement(16267..17685)
                     /locus_tag="PKI68_00250"
     CDS             complement(16267..17685)
                     /locus_tag="PKI68_00250"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNX52152.1"
                     /translation="MYAAPSISETLFPRAAAYRRQQPAEAVCETAGRWFPYTERHLHA
                     LWFDERLRPQNLKTSHGEPVAVENPGRWNLEAGPDFSGAVLLIGREKRRVAGDAEIHI
                     FPNGWKSHGHANDPRYSGVRFHITWFSGKIDESLFPPGTVHIPLADVCAADLESLDLS
                     AYPYHEPHAAAEFPLSRKSPDAISRILEGAGEERLRQKTLRMAWLIRQRGEAQALYEE
                     TAAALGYKHNKTPFRKLAQSLPLEALAQYGDDWKPVYAVLLGISGLLPKQPGSGWPQA
                     SKTELRSLWDCWWREQHKWEEIAPLHKNEWRLAGVRPLNHPVRRLAALSQWVASGTFQ
                     GRARSPNAPPVLISVEGRTARRAVPTQFPKCFWSTHTGWSGKEKPAELVGADRLHAIE
                     LNVFVPCRLAKGDGSVLRNLPVEPLNSVIRETAYTLFGPDHSPKLYRTAFARQGLIQI
                     FNDFILTGRLTEFWQTDKEVIA"
     gene            complement(17690..17974)
                     /locus_tag="PKI68_00255"
     CDS             complement(17690..17974)
                     /locus_tag="PKI68_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNX52153.1"
                     /translation="MVEVKAVIKNHAGIHCRPTAVITQAVAGVKSTVTVIAPSGRCQL
                     GSALELMILGLELGTQITIQAEGPDEAAAAAKFKELFETEFDFPNAGHGG"
     gene            complement(18021..19064)
                     /locus_tag="PKI68_00260"
     CDS             complement(18021..19064)
                     /locus_tag="PKI68_00260"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HNX52154.1"
                     /translation="MKKVIFFDAKPYDREFFDAANAKHGYEIKYIGNRLSADTAPLTA
                     GYDAVCAFVNDDLSSPVADILFNKGIRVIAMRCAGYNNVDLKAVYERIHVARVPAYSP
                     YAVAEHAVALIMGLNRKTHKAYNRTRDSNFALGGLLGFDLHGKTAGIIGTGRIGQILA
                     GILKGFGMRVLAFDRFPKTDWAAGAGVEYVPLEELYRQSDIISLHCPLMPETQHMINA
                     ASLAQMKDGVMLINTSRGGLIQTKDLIDALKTGKIGSAGLDVYEEEDGYFFEDRSGEV
                     LTDDVLARLFTFPNVLVTAHQAFFTKEALCNIAETTLSNLDAFFRGETMANEICYRCN
                     KDGCLKKQSGKCF"
     gene            19212..>20932
                     /gene="pheT"
                     /locus_tag="PKI68_00265"
     CDS             19212..>20932
                     /gene="pheT"
                     /locus_tag="PKI68_00265"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNX52155.1"
                     /translation="MKFALSWLKEYVDFDDTIAGLSDKLTFAGLEVEHIETLGGTFEG
                     VVVGEVKQIEPHPDADKLRLCTVEYGAAETLRVVCGAPNVAVGGKYPFATVGTTLPCG
                     IKLKKAKIRGVESLGMLCAKDELGLGEDHSGLLVLEASLKPGTPLAAVMGPAETVFEL
                     EITPNRPDCLSIIGIAREVAAIYGKPLKMPAIELKESAEPVEKEISVEVNDSVKCPRY
                     TARVLKDAQIGPSPDWMKRRLEACGIRSINNVVDITNYVLLETGHPLHAFDYTLLKDG
                     KIIVRHAAAGEKFTTLDGVERQLTGDMLVIADAGKAVALAGVMGGANTEIKDSTTTIL
                     LEAATFEQSGIRATAKALSLYTDSSYRFQRGVSAGSVEWASRRAAALMSELAGASLCK
                     GVVDAYPAPKAKRQITFRWQSACELIGMNVPVETMKKIFQSLEISILSSDSEKAVLEI
                     PAFRDDLEREVDIIEEVARMNGVDKIPEKLPVAETVAGVNDNRIRAITACRKNLVGLG
                     AREIMNYTLVSHPLLDLFGASDRAAREELPHPISAEQSVLRTSLIPQMIESLGRNKSR
                     QIDEAVF"
     assembly_gap    20933..21032
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21033..21493
                     /locus_tag="PKI68_00270"
     CDS             <21033..21493
                     /locus_tag="PKI68_00270"
                     /inference="COORDINATES: protein motif:HMM:NF015129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52156.1"
                     /translation="GRAAAVFSGKEKIGVLGLINSGARKEWRLNDPVAAAELNLEPLI
                     ANVWKRSEVKDIPLYPAIERDFAFVVDEAIRHEDILAAVQSAAPAELESVKLFDIFRG
                     KALEKGKKSVAYSFVYRSPAGTLTDEKVNAFHEAVGKKVCAAVAAVIREG"
     gene            21855..23168
                     /locus_tag="PKI68_00275"
     CDS             21855..23168
                     /locus_tag="PKI68_00275"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNX52157.1"
                     /translation="MELFEEKPSASLKPGAGTAPLAARMRPRTLDEIAGQEHILGAGK
                     LLRRAIEADRISSVILYGPPGCGKTSLAEVIALATDRRFERTSGVLANVAILRKLLEQ
                     AAGWKKIHKQDTILFIDEIHRFNKSQQDVLLPYVESGDIILIGATTHNPFFFINTPLN
                     SRSQIFQLNPLSEEAIKTVLKRAMEHPAGLNGAVTADDDALAHLATVCEGDARRALNA
                     LEIAALTTPPGEDGIVHLTRHETEESVQKKAVSYDHDEDEHYDTISAFIKSVRGSDPN
                     AAVYWLAKMIYAGEDPRFIARRLVILASEDIGNADPRGLTIATAAVQAVDFVGMPEAR
                     IILAQATTYLACAPKSNASYLAIDAALADVENGRTLPVPDHLKNAPHPGKGEVQYQYA
                     HDGKDHFVDQEYVPTDRIYYAPTDQGYEETLRKRLEYWASRRKKK"
     gene            23232..23834
                     /locus_tag="PKI68_00280"
     CDS             23232..23834
                     /locus_tag="PKI68_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNX52158.1"
                     /translation="MKQWPHAPLHILEERGTYMVTCGTYQKCHHLNEASRLTFFQNAL
                     FEIAEEFGWRLQAWAVLSNHYHLIGSSPEHPGTLQKMLSKLHTVTAIQLNKEDNEPGR
                     KVWYQYFDSRITYQNSYFSRLKYVHQNPVHHGVVHNAETYPWCSAGWFARSAGSAFYK
                     TIQGFKTDSVNVTDPYDTIPINLEIESGVKPPHSKQECPS"
     gene            23831..24376
                     /locus_tag="PKI68_00285"
     CDS             23831..24376
                     /locus_tag="PKI68_00285"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNX52159.1"
                     /translation="MTKAEIRARIAAQKKTVQGLEAPSAAAVKNLQYLAAFQSARTVG
                     AYMPLPGEVDISPLFACPEKIFFIPAFDESSGLYRMARLAGELKTGRFGIREPAVPVF
                     AEEHDLDLIIVPGVAFDRAGQRIGRGGGFYDRLLPQYRAIRTGICFDFQCLEVIPAED
                     HDVRMDFTVTESKILKFAMNS"
     gene            24442..26013
                     /gene="rny"
                     /locus_tag="PKI68_00290"
     CDS             24442..26013
                     /gene="rny"
                     /locus_tag="PKI68_00290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNX52160.1"
                     /translation="MDSIGLWWGDMQNFVLSIGFVVLGFFFHAYLTRTNAVAASHRGS
                     AIMDDARKQAEAIKHEADVSAREEILKARERAEKEQETKRQSIIALEDRVVAREKDIS
                     RKLELLEAKDRTLAEKLDKAEQQSEELKAQQKTLKDLIEAETRKIQEVASLSKEDARK
                     LIMDQMEKELEGEISGLIRHQQKEIYERARAEARDIVCTAIERYAGEHVSNITVTQVA
                     LPSEDMKGRLIGKEGRNFKSFETESGVNLIIDETPGIVQLSSFDPVRREVARVALEKL
                     MLDGRIHPASIEETIKKTRSEIDEIIRQSGEDALYKLGISGVAPELVKVLGRLKYRTS
                     YKQNVLDHSMEMASLMGMMAADLGLDVALAKRVGIFHDLGKAVDHEVEGSHAIIGANL
                     LRKYGEVPLVYNAVAAHHEDVEKSSVYAVLAAAADAITAARPGARMDTTDLYFQRLDK
                     LEKIAMSFKGVDKCYAIQAGRELRVMVEGAKVDDARAMILAREIANRIQDEVKYPGQV
                     KVTVIRETRAIEYAK"
     gene            complement(26023..26430)
                     /locus_tag="PKI68_00295"
     CDS             complement(26023..26430)
                     /locus_tag="PKI68_00295"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNX52161.1"
                     /translation="MIRLLQWFKKEAPHLRTGRRGEDQAEKFLKKAGLKIIARNVRIG
                     ADELDLIAQQGDTLIFVEVKTRANEDFGRPFTAVNRAKRRKLSRAAIHFLKKRKLRPP
                     YIRFDVIEVVGEKPEIRHIQNAFTLEGGCRIWW"
     gene            complement(26427..27065)
                     /locus_tag="PKI68_00300"
     CDS             complement(26427..27065)
                     /locus_tag="PKI68_00300"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNX52162.1"
                     /translation="MLRYEKEAWQSGLFFIAGVDEAGRGPLAGPVVAAAVAFSREPLE
                     NGGAAAFTGLTDSKALTAKQREVFFEQLQACSFARIGCAVIGPDVIDEINILQATWRG
                     MADAVAQISPQPDLALVDGRPVRGLCCKSQSIVKGDAQSLSIAAASIIAKVTRDRLML
                     GLDAEFPQYGFAKHKGYGTAEHLAAIRRHGPSPHHRRTFRPVSELSQGELNL"
     gene            complement(27078..27425)
                     /gene="rplS"
                     /locus_tag="PKI68_00305"
     CDS             complement(27078..27425)
                     /gene="rplS"
                     /locus_tag="PKI68_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNX52163.1"
                     /translation="MNPVVLKIGKEQCTKEIPAFNVGDTVNVHVKIKEGTKERIQLYT
                     GTVIGREGAGVAETFTVRRVAFGEGIERVFPMQSPNVAKIEVVRSGQTRRAKLFYLRD
                     ISGKKARIKERRV"
     gene            complement(27489..28187)
                     /gene="trmD"
                     /locus_tag="PKI68_00310"
     CDS             complement(27489..28187)
                     /gene="trmD"
                     /locus_tag="PKI68_00310"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNX52164.1"
                     /translation="MQIDVITIFPEMLAGFFGQSMMRRAAKAGHVTFNCINLRDFAED
                     KHRTTDDRPFGGGPGMVMKPEPIFSAVESIRTERSRVILMCPQGKPFTQRRAQTLSNE
                     EHLIFVCGHYEGVDERVREALIDEEISIGDYVLTNGVLPAAVVIDAVVRLIPGVLGGE
                     GAAEQESFSEPLLEYPQYTRPPEFRGMKVPEELVSGDHAAIAKWRRRQAEKRTAERRP
                     DLLSGCGAENRDGA"
     gene            complement(28237..28467)
                     /locus_tag="PKI68_00315"
     CDS             complement(28237..28467)
                     /locus_tag="PKI68_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNX52165.1"
                     /translation="MKKLVKQMVAALVDHPRDVKITEVKGDKSIVYELRCHEKDVGKV
                     IGRSGKTVGAMRTLLGAIAARDGRRAMLEVVE"
     gene            complement(28470..28766)
                     /gene="rpsP"
                     /locus_tag="PKI68_00320"
     CDS             complement(28470..28766)
                     /gene="rpsP"
                     /locus_tag="PKI68_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNX52166.1"
                     /translation="MATKIRLRRMGTIKKPFYRIVVADSRFATTGRFLEALGWYDPKQ
                     KGDNFSVNVERVKHWMGTGAQISNTAASLLKKAEAGQGVPVGAADQKASESQGA"
     gene            complement(28870..29967)
                     /gene="mutY"
                     /locus_tag="PKI68_00325"
     CDS             complement(28870..29967)
                     /gene="mutY"
                     /locus_tag="PKI68_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNX52167.1"
                     /translation="MKTDRLPEVRQAVRNSLLPWFAANARDLPWRRNRTPYRVWVSEI
                     MLQQTRVDTVIDYYRRWMKTFPSWKALAAAPQSDVLKCWEGLGYYSRARNLHAAAKTI
                     AGEYAGKPPAAVAELAKMPGIGPYTAAAIASLAFNTDAAVVDGNVIRVLSRLFAFGGD
                     TRDAKGKKQMQARADDLLVKGRAGEFNEAMMELGALVCLPKNPKCPECPMQSVCFAWA
                     GGAPEKFPVTKKKPQVPHITVGAAVTLNRKGEVLIAQRRQNDMLGGLWEFPGGKQESG
                     ETIQECIARELKEELGINTAVGEFFLTVKHTYSHFTMTMHVYFATVISGRPRPIHCAD
                     YAWVKASELSKYAWSKADLQVVEKLQNRTAG"
     gene            30086..31153
                     /locus_tag="PKI68_00330"
     CDS             30086..31153
                     /locus_tag="PKI68_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52168.1"
                     /translation="MCKKLVLLTVVLLASLTTVQAAWKSNITMLVVPEEKIPLQIAQD
                     LSRRYPVLLVSYRLARGEAKLHAWNGDTWIPVSAEDYANGTFFANRPSHAIIVENERV
                     HVPLALTPNSTWCTTASRLTTTDPRILLHLFGRYFNFPFRYWDQFAQRYSYTIEEINP
                     TLENVYWWNRYSRTKDKNSARLALAADMNKWTPIALLPPPRIEPVTLEKEPVIPEVEK
                     PATEPVDGAVDITVSAPEPKVEKKPDTKPVIKPEMPASKPFPSLKPEPVIKPAAAAEK
                     APAGEPEPVIESVPAIEPAPAPLVEAPATAPAAEIEKTVNAEVPAPAPLVTEVDPFAA
                     DEVPAAEIVVPQPPKKPWWKF"
     gene            31153..31476
                     /locus_tag="PKI68_00335"
     CDS             31153..31476
                     /locus_tag="PKI68_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52169.1"
                     /translation="MHKLFILLTALILAGCTTSPAPFEFPVSPRLATSYTNGETIMTW
                     KALSNQTYTVYYTDAPLGKLADWKPLPQANKLSGSGKQISVTDKTGADVPRRYLLLTG
                     DQKPY"
     gene            complement(31480..33783)
                     /locus_tag="PKI68_00340"
     CDS             complement(31480..33783)
                     /locus_tag="PKI68_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNX52170.1"
                     /translation="MHELKFLQDLAVVLAVAGIAGWLCQRIRLSVVSGYLLAGVLLGP
                     AGVVTLVSDSESLRLLSQVGLIFLMFSIGQNLGIRQIRNSGIASLTATAVSTALLFTL
                     CRGIGHMLGLGKVGGLLLAGMFITSSSVIIGRLLHESGRFHRATGQAAMFQTLLEDFN
                     VIILLSLLSSQLHDGPDVSLPVLQQLGAFASFVVLLAILALLFIPRILHLLQQSVRPE
                     LRNVILAAILVGAAVAARSAGYSLALGAFVLGMAVAGTPDRPQIEKAFSGLSDLFGAV
                     FFVSIGMLIDIRHIFTAWPLVLLFTALAVFGRSAICTVGQLAAGRNLRDAVTTGLVLI
                     PIGEFSFLVAQIGVGAGRLPETFGAVIVGVSILSTMASSVLLPVSGRLADGLVACQPR
                     WFSQALAAYQNGLNRLAARRSSDMVWKISRKCLLRIAVEMLFVTGLMVFSPKLHAMFD
                     GVASGQNWMRWVFNLVFWGPFGLIVLIVLIAVWRNIEVLTMLHSEVAARRYTRRETRA
                     AAHTIEQALRTVSLLLLSAWLWLLLPFALSGFWTFIGIASVIVLSGALLWKPMAHLHS
                     VAENRLQRHAAAGQQVPAALILRHDSDWDLNIEEVSIPQSSLCRGRSLAQLDLPNRFK
                     CSVLGVERQGMVIDAPGSGFTVFPQDVLLLLGEPSQIPAARAFLNSTCMAESERLDEI
                     RLDTLTVPEAVSFADRTLAEWNIARQTGVQIAAIRRAGKRIVTPGPAEHLYAGDELLV
                     LGTVEKIRQLEFLLINAEADKPEASPS"
     gene            33881..>34176
                     /locus_tag="PKI68_00345"
     CDS             33881..>34176
                     /locus_tag="PKI68_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52171.1"
                     /translation="MRLKKTALLLIAGLLTVCTAIPAFAGVRTVTSRGKNYVTLPNLA
                     SYYGMTISQPSGKRIRLQNKFNRIEFETDERSVWINGTLVALSEPVRKVGWSWA"
     assembly_gap    34177..34276
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <34277..34555
                     /locus_tag="PKI68_00350"
     CDS             <34277..34555
                     /locus_tag="PKI68_00350"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNX52172.1"
                     /translation="GNRYDAANMALSYRIQQNLIRATGQEDRGVKRARFEVLRDAPCP
                     ATLVEIAFMSNPKDEAIVMDPAGRDRIARGIADGIVAYLTDARRSKTK"
     gene            complement(34559..37594)
                     /locus_tag="PKI68_00355"
     CDS             complement(34559..37594)
                     /locus_tag="PKI68_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNX52173.1"
                     /translation="MNMIFNASAGTGKTWQVTELYSALVLGRNHEQLPPGRPPVAPEK
                     ILLMTFTDNAAAELRSRVSGKMLSVEKSALGSDDELADRARRVLRSLPAAYISTIHAF
                     CAGLLREHALELGLSPAFKTLDDEERDALLDEVLKAEIFKSLEADADFREFCAGVAIL
                     GAPDNSYSLINTVRALLEKAAGRGLDLCNAEAMLPVPERTVRKQSFERICAELETFDL
                     PKTAADALDALKAALADFPNPARIAAIPKFGQAKKVKHLSDELAELKERFLTEYFYEQ
                     NAGKFRAFARCLSACARGFDAAKRSRDAVDFGDQLLLARDLLRRGDFSGRFDWIIVDE
                     VQDTSRVQCEVIEALWGKETRLVVCGDRKQSIYAWRSADPQVMPDLEAAMTARGGVRR
                     IDLKTSFRSNDRVISAVNALFSGIYERYDALEPQGVQSGEKPCVEFLMQDGDESTTEH
                     EMAAVARRIRLLVDGGAEWRPKFGHNGRTFAEGEPFRYGDILILLKRSTHQSVLEKAL
                     RDAGIPYTSGGKGRALFEQQEVRDLLLFLQTLCEPANDLALVGFLRSPFANVPDETIV
                     RLGWDGETFDHEILRRQFFESGSEAAERLLRYRALSGQKLASQLIRDIVRETAFDAQL
                     AGLPGGGQRLANFKKALDWIRAAERGGQVLLPDVVRRFEKYIQTPPRSGAAEALLPDP
                     EQNAVTLMTVHGAKGLTQRVCFVPDISFGENSEPGFAMFSPEGRLEMKLSGLAGEEVL
                     SPGWEAARAADKTMRELELTNVFYVAMTRARDLVVLSGAGTQEPKGWLKLSEAFLQSA
                     SPDLLRRLRFDEIPESEGVRPEALGFRQEIEFQPLQIPKGLERRTVTSLVQALKPKTQ
                     SPQPAEDRRAFGSLGHTVLEELAKNKWNGDIPALVEMFSREFGAVESGRLIRQLEAAR
                     ELLRRETAGAAALLAEYPFVLKRGDVILDGTMDLLVQFPDRFRIFDYKFSGELPENAL
                     NVYAPQLAAYKEAVEKLHPGAAVSATLVLIGESVRTAVL"
     gene            37746..39161
                     /locus_tag="PKI68_00360"
     CDS             37746..39161
                     /locus_tag="PKI68_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNX52174.1"
                     /translation="MRNPHDVNSRKTLKALSAGTALLTLPLLLPADEPVRPPNIIYIL
                     ADDLGYGDLSCYGQKKFSTPQIDRLAQDGLRFTQFYAGSAVCAPSRCSLMTGLHTGHA
                     PVRANREAKSEGQAPMPEGTVTLPALLRQAGYVSGMFGKWGLGAPGSSSDPMVYFDEF
                     FGYNCQRQAHSYYPDHLWHNRETVPLDKKTYSHDLILKASFDFIRAHRNEPFFCYLAV
                     TIPHAAMQAPPALHEKYRKLFPQFENLIGKYDGAQVQNPVAALPAMIEHLDTGVGQLM
                     ALLNELGIDRNTLIIFTSDNGPHKEGGNNPAFWNSSGSLRGGKADLYEGGLREPFIAR
                     WPGVIAPGTVSDLPAAAWDILPTLCELARTAPPADIDGISLVPALKGQPQPAHDYLYW
                     ELEQQGGQQALRKGNWKAVRRNILKNPQAAIELYDLSKDPAETTNVAALHPEIVQTLT
                     TLLQQARTESKTFPLFPAPKP"
     gene            39425..>40729
                     /locus_tag="PKI68_00365"
     CDS             39425..>40729
                     /locus_tag="PKI68_00365"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNX52175.1"
                     /translation="MKFDLLIKNGTVLDGTGAEAIHADIGICGERIVEIGDLSKAEAR
                     QTIDATGQIVCPGFIDVHSHSDAYLLIEPRASSKVFQGVTTEVCGNCGASAAPRLGDA
                     KLPSDWQDKPFPGTWRTVAEYRRLFEQVKPAVNEALLIGHNTLHRGISGYEPRGATPD
                     ELKEMCRALEQALDEGGCGLSTGLIYPPASAVPSDEIIALAKVCAGRNKIYTSHMRSE
                     GRQLLEAIDEALMIGRTSGARVQISHLKTSGKDNWHKLDAALAKIEAARAEGIEVAAD
                     RYPYTASCTDLDITLPGWALYDGKAAVLARLADADTRKKIRAEILEGRGEFGFGIIMI
                     GSTEHPDLQRFKGLYLPDAAKELNMEPVDALLHIVEKDELRTGGIFFGMSEENMWKIL
                     AQPWVMIGCDASIRAPDGILSKDHPHPRAYGSFTKVLRAALDG"
BASE COUNT         8790 a        12491 c        11312 g         7936 t
ORIGIN      
        1 tccatgattt tttgcgctcg gcaaattcgg catccgaaac gtcggctttg agcgaacggt
       61 tcggaatgtc gatttcgatc aggtccccgt ttttgagcag gccgatcggt ccgccttccg
      121 ccgcttccgg cgaaatgtgg ccgatacagg cgccgtgcgt tccgccgctg aaccgtccat
      181 cggtaatcag cgcaactttg tcgccgagcc cctgccccat gatgtagctg gtgggcgcga
      241 gcatttcctg cataccgggg ccgcctttcg gcccttcgta gcggatgacg acgacgtcgc
      301 cggctttgac ctgaccggcc agaattccgg cacaggcttc ttcctgcgat tcaaaaatca
      361 ccgccgggcc tttatgcacc agcattttcg gatcgacgcc ggcactcttg acgaccgcgc
      421 cttccgcagc gatgttgccg cgcagaatag ccagtccgcc ttctttgctg taggcgtttt
      481 cgaccttgtg aatgcacttt tcgtttttgc tgaccgctcc ggcgatgttt tcgcccaggg
      541 tttttccggt gaccgtcggg cggtcgagat tgagcagtcc tttaattttg ctgatctcgc
      601 cgagaatggc gctgataccg cccgcggcct gcacgtcttc gatatgccag ctggaggagg
      661 gcgaaacctt gcagatgttc ggcgtggttt tggagaggcg gttgatccgg tcgaggtcat
      721 agctgatgcc cgcttcgttt gcgatcgcca gcgtatgcag cacggtgttg gtgctgccgc
      781 ccatggccat atcgagaata aaggcgttgt ccaccgactc gagcgtgatg atatcgcgcg
      841 gtttcgggcc gttggcttta gccatttcga cggcgcggcg cgcggcttct ttccacagct
      901 cgcggcgtgc aggatcaatc gccagcagcg tgccgttgcc gggcagagcc atgccgatcg
      961 cttcacacag gcagttcatc gagttggcgg tgaacatacc ggagcaggac cccgcgccgg
     1021 gacaggccgc gcattccagc tggcgcagtt ccgcttcgtt gattttaccg gctttgcact
     1081 gggcaacgcc ttcaaacacg ctgatgaggt caacgacgcg accgtcggcc agccggccgg
     1141 ccgccatcgg gccgccgctg gcgaaaacag tcggaatatt acagcggacc gcgcccatga
     1201 gcataccggg gacgatctta tcgcagttcg gaatacagat catcgcgtca aaggcgtgcg
     1261 cctcaaccat cgtttcgacg caatcggcaa tcagctcgcg gctgggcagc gaaaacttca
     1321 tcccctcatg gcccatcgcg atgccgtcgc atacgccgat gacgttgaat tcgcgcggcg
     1381 ttccgcccgc ttcgcggatc gcttcgcaga tcagccgtcc gacctgattg aggtgaacgt
     1441 ggccgggaat gacttcgtta aaggaattgc agacggcgat gaacggcttc ttgatgtcct
     1501 catcagtcag tccggtggca tgcatcagac tgcggtgagg tgcgcgttcc attccttttt
     1561 tgactctatc gctgttcata acgaattcct ttccttaaaa aacgatcgac tttatcgtat
     1621 attcaggtag cttgtccgcc tttaaacctg aaaacaccga aagcatacgt tccgggaaaa
     1681 tattatgaag acaattttta gcgcaactgc agctctgctg gccgccggaa tcctcctcgc
     1741 cgatgagcct gccaccaata ttccgccggt tgtcgtgacg gccgcggcgc agacagctca
     1801 aaacgcggcg attcatgaag tactgcgcgc cgagccgggt gtggtgctga attcgcaggg
     1861 cggctcccag aatgaccttt ccgtccgcgg cagctcgttc agcggcgcgg gtcttgcgct
     1921 gggcggaatg acactgcgca atccgcagac cgagcatttt aatgccgaac tgccggtacc
     1981 ggccgcgatt ctttcgcggc cgaaggtcat gaccgggctg gataatcagg gcggccacct
     2041 gaccggtacg gtcgggtttg atcttcttcc catcaaaggg aaaaagcagg ttgaggccgg
     2101 attcggttcg gatcaccggg actggcagag cctgctggtg caacagatgc tcaccgacaa
     2161 tctcggtatc ggcgtgttcg gcggacgaga gtcggccgga gccgttgatt acaaggataa
     2221 cgactacgac cgcgagtata ccggcggaca tatgcagtac cgcgaagatg atacgcaggt
     2281 ggacactctg gtcgctcacc agcaaaaaga attcggtgcg cgcggctatt acggcgtaac
     2341 cgacacgatc ccggccaacg agaaaaccga agatacgctg gcttacctcg ccgcccgcaa
     2401 aggcgatctg aatgccgact atctgcgcgg cggcgtttcg tggcacgagt ttcatgacga
     2461 ctatgaactg ccgacctatc tttaccgcaa ccagcaccgc tctcaggtca gctcggcctt
     2521 ttttgacggc cgcacactgg aagtaaacgg ctgggcgctc ggctggcgcg ccgacgccga
     2581 tcaagaacgc atcgacagcg acggttccgg cggcctcgga caccacagcc gctcgcacgg
     2641 cggcgtttcg ctgctgccgc agtggcaggg cgaccggctg aaagtcaccg ccggagcgcg
     2701 cagcgagttt ttcaccggcg aacagcccta ttatctgccg caggccggcg cggaatatat
     2761 gctctccgac acgctgacca cctttgcgtc ctacaccgaa acggtgcggc agccttctta
     2821 taccgagctt aattacaact cccctttcag cctcggcaat accggtctcc agccgcagac
     2881 cgagaaacag accgaggttg gatttaaagg cgttccgtcg gaatataccg actggaaggt
     2941 cgcgacgttc taccgccact ctgaaaacac agtggactgg atgaaaacca cttccgggtc
     3001 ccgctggaac gccgtggata tcggcgatct ggatgtgtac ggcacggagg cccggctcgg
     3061 ctggtatccg gtacaaacgg tcgaaatgca gttcgcttat acctggattt acaaaaataa
     3121 aaccgccgag gacttcgact cctatgcttc gcgctacgcg ctcgactatc ccgagcacct
     3181 tgcgcaggcc tctctgctct ggcgtccggt caagccggtt gaaatcggca cggtgcagtc
     3241 gctccgctgg cagaccgata acgaggtgcg ccggaacggc gacttcggcg cggacagttc
     3301 gttcgtcgtg cgcttcacgc cgccgaaggc ggattacgcc acgctttcct tccttttgaa
     3361 caacgcctgg aatgatgact ttcaaacatt ccccggccag cgtccgcccg aacggttcgc
     3421 cggggtttcg ctgacgctga cgtggtaatc cggacggctg tttcatcacg tcacactgag
     3481 cctgcgatct gcgaatttat gacgcaccga ctccgtgcag tttaaagaaa ccaccttgat
     3541 tttcagggcg ttacgccgct ggcatatcca ctgcttttat ctcaaccgga tccgggacat
     3601 ggggttccgg aaacctaaaa ggagggttct tatgaactgg ccaaccaaaa ggaatgacag
     3661 ctatccggtt gcatggaaca ccggtctgct gcaccgctcc attgacgatc tccttgcaga
     3721 ttttttcggg ggatccgcaa tggaacaggc gggactgatt acaccgcggt ttgaagtttc
     3781 agaaaccgat gacgcggtta tcgtagaagc tgaactgccg ggaatggatg aaaaagacat
     3841 cgaactgacc ctgcaggaca gcattctcac catcaaaggt gaaaagaaaa aagaggaaga
     3901 aaccaaaaag aaaaactgct acatctccga acgcagttat ggacgcttcc agcgctcgct
     3961 ccagctcggc tcgggcatcg acgaagacaa agtcagcgcc gctttcaaga aaggcgtact
     4021 gacggttacg atcccgaaat ccgaaccgga aaagagcaaa acgcgcacta ttaacattaa
     4081 agcggaataa gtccgtaaaa acaaaaaacc gctctccttc gggagggcgg tttttaaatc
     4141 tggtggcggg acccggaatc gaaccgggga cacggggatt ttcagtcccc tgctctacca
     4201 actgagctat cccgcctcaa aaaagaaggg cgcattctac gtaaaagacg catgatgtca
     4261 aacggaacta tcatcagcct ttgaacttgt ttcagccggc gctcctatct acccttccgc
     4321 ccatgtatgt gctcgacagt ctgctgcccg tttttctgat cattgctctc ggactgctgc
     4381 tccgccgcac cggattcttt tctgagacgc tcgccacggg cctgaaccgc ctgacgtact
     4441 gggtcgcgct gcccgccctg ctgctcgaca aagtcaccaa cgccaccttt gattccgggg
     4501 atgtcgcccg gatgtctttg attctggtcc tggcgaccat cggctcggcg ctggttgccg
     4561 gtcttgccgc cttcctgctg cggctgaacc gccggtcatc cggcgctttt attcagggcg
     4621 cggcccgctc caacaatgcc tttatcggcc tgccggtcat tctctacgcc atgtccagcc
     4681 tggcgcccgg cgtcgaagcg ctggcaacgg tggcgctggc cccggccatt gtcttctaca
     4741 acattctgtc ggtttccgtt ctgctgatgc acagcgaccg gaaaacggcg aacgaacatc
     4801 agaccggcgt gctgttcgcc aaacagctgc tctgcaatcc gctgctgctc tcctgcgccg
     4861 ccggactcat ctttaactgc tgcggcgtcc attttccgct ggcgctcgga cgttcgctga
     4921 gtgtgctggg cgattcatcg ctggcgctgg cgcttctgag catcggatcc gcgctgacct
     4981 tcaaaggagc ggccaaaggc ctgctccatt caacgagcgc tgcggtgatc aaagtgttca
     5041 tccagccgct gatcggcctc ggtctggcga tgctctggaa cctgccgccg attgaacggc
     5101 agatgctgct catttatctg gcctgtccga ccgctgtcgt cagctatgtg ctggcggata
     5161 tttttgacag cgaccgcgag ctggcggcgc acatcatcgt catcagcaca ctgctttcaa
     5221 cggtttcgct gagtctcatc gtcgcattcg gaggatagaa aatgaccgta cccagaagac
     5281 gtttcggaaa aacagaactg caaatgccgg tgatcacctg cggcggcatg cgctaccagc
     5341 aaagctggca ggatgacgac gcagcccgcc aatcggtcac cgaggaaaac cagaaaaacg
     5401 tcgaagccac cgtccgctac gcgctcgacc tcggcatcaa ccatatcgag accgcccgcg
     5461 gctacggcac ctccgaatac cagctgggaa aattcctgcc ggcgctgccg cgcgatcaaa
     5521 tgatcgtgca gaccaaagtc ggcccgaagg atacgccggc tgaatttctg gaaagcttcg
     5581 acacctccat ggccgcgctc aaactcgact acgttgacct gctcgccatt cacggcatca
     5641 acaacgaaga gacgttccag caggcgctca aaagcatgcc tgcgctcgaa cagctcaaaa
     5701 aagagggccg ctgccgccat atcggttttt cgacgcacgg cacgccggac atcgtcgtca
     5761 aagccatcga aaccggcctc tttgaatacg tcaacctgca ctggtacttt gtctatcatc
     5821 cggtcacctg ggcgtcggtc gaagcggcga cgaaactcga catgggcgtt tttgtcatca
     5881 gcccggtcga taaaggcggc aaactgtacg agccgccggc gaaactgatc gacctctgcc
     5941 agccgatcac cccgatggcc tttaacgacc tttactgcct gcgccgtccg gaaatccaca
     6001 cactttccat cggagcttcc cgcccgtccg actttgtcga acacgtcacc gccgccggaa
     6061 atatggacaa aaaattcccg atcgttgaaa aaattgaggt gcggattttc aaagccatgg
     6121 aaaaggaact tggcgccgac tgggttcacc actggcatga gggtctgccg cgctggcagg
     6181 acacgcccgg cgaaatcaac attccggaaa tcctgcgcct ctggacttat gccaaagggc
     6241 tcgacatggt tgcgttcggc aaaatgcgtt acaaccttat gggcaatgcc ggacactggt
     6301 tcggcggaat gcaggccgca gaattcaaag atgaagcgct caaaccgtta ctggaaaaaa
     6361 gccgcttcgc cggccgtatt cctgaaatcc tgcgcgaagc ccacgcgatg cttcacgacg
     6421 aaccgaaaaa gcgtctcagc gagtcgtgat tagaaattat aggtcacgaa cagccgcgcg
     6481 tcggtcgcag tggtgtcttc tgtgccgcga cacccttcgc gatagccggc gccgactttg
     6541 actgcaaccg acaggctgtc tgtgaccgga tgtttgacga aaaagttgat ctcctgcccg
     6601 tcgaaagcgt gccggctgtg atcctgccag gtgtagctgt agagcgtata aagagcggtc
     6661 ttgccgatct ttgaagtagc tttcaaatag gcgctgtctg atccgccgtt gaaaggcccg
     6721 gaatagatca gcaacggagc gccaagcgga tgattcagcc cggtggttgt ctcatgaaaa
     6781 atcatatcgt cgccgtgaac gctgaaccag cccggctcaa acgtgacgcc tccggctttc
     6841 tgttcgaaag cgaggctgaa catatcggaa ttaaacggcg cgattttatc cgccgtctgc
     6901 accgagtccg cgtgctggaa atagcccagc agcgcggttg aatccgtcag cgcgtagcgc
     6961 gtgcggccgc cggtaacgtt ggcgatgtcg tgtacatagg cgtcatacaa agccacttcc
     7021 agattggtga cgccggtaaa caccgcttcg ccccatgaaa taccgggact gccgcaaccg
     7081 ttcccctgcg gggccagcac ttcgtcgaag tcgttgaacc tccagccgtc ctcattatcc
     7141 agaacattgc tgagacggaa ggcatgtccg gccgtcagcg aaaggttcgg aatatctttc
     7201 gtcgttagct gaaccgcttc aatcgaacgc ggcttctggc ggatctcatc ggagcggaac
     7261 agttcgccgt tcatgacctg ccgcccggct ttaatatacg tattcgtcag accgaggctc
     7321 tcaaaccggt atttgaccca cgcttcgttc aggatattca cccgtccgtt gctcaacagc
     7381 gttttgccgt tattactgtc cctggtggcg tccacgggtt ccacatagat ccaggttgca
     7441 ccggcactga atccggccag ctccggcgag atatagccga tcagcaggcc cgctgtcgtg
     7501 gaataggccg tgtcaacctt ggcgccttca acatcgcggt acatgctcag cgtttgcagc
     7561 cgcgcattcc aggtcccgta accgagatca ttcaccggct tcagcaggct ttcagcagat
     7621 gcaccggtac acagagccgc cagcaataca gacaacagcg ttcttttcat catgacaaaa
     7681 ttcccctgcg ccggattcat aaaagcgcgc attcttatct tttgctccgc ctgcctgcaa
     7741 accctaatcc ggcggagcag gaatcatttt gacaaccgga cggagaaagt ggattttcgg
     7801 cgcctttcaa tttaacagga gtaaatcatg gccgaattta aaaacgtaac cgtagtgaaa
     7861 aaagcgaata tctactttga cggcaaagtc accagccgca ccgttctgtt tgccgacggc
     7921 accaaaaaga ccctcggcat catgatgccc ggtgactatg aattcggcac tgaagccgcg
     7981 gaaatcatgg aaatgctcgg cggacagatg gacgtactcc tgcccggcga aaccggatgg
     8041 aaaaccttca aagcgggcga atcctatcag gttccggcca acagcaggtt taagctcaaa
     8101 gtcccggtcg tcgccgacta ctgctgttcc tacctgaaat aattttatcg cgccggaaca
     8161 aactcaaaac cggagatcgt cagatcaacc ggcgcggcgg tgccgtcgcc gaatgtcagc
     8221 acgatttccc ggacgccttc cagccccggc gtcgcgggca tcggaatccg gattcccttt
     8281 tcgctcccgt ccggatcgaa ccgggcataa atcccgttct caaaggcaat cggcgcgcct
     8341 ttgagcgtaa gaaccgcatt ctttaccgga gccgccgcgc gatagcggat cagcagcgcg
     8401 ccgttcgaca gactgacgct ctgcggaagc ttaattgtaa ccgcgccgcg ctgcaccgcg
     8461 tcgccgcgca cacggaaacc gttcatccag tgcacgcctt ccagtccgtc gccggaagga
     8521 ctccaggtaa tccacttcgc gccgccgctc aacagatcaa ccgcagacga ttcaccgccc
     8581 tgcactttgg aaagcgacgt cacgccccgc catgtcaggt agcgctgcat attttcgccg
     8641 gcagaaccga tgccgcccag aaccagcgcc agcgtgtgcg ccgtggtctg aaagcggatc
     8701 acttctttct tcgccgtatt aaatccttcc cacggcccgt gatctgtaaa cagccggcgg
     8761 atcgttttcc agttactcgc aagaaacgct tcgaccttgt ccggcgcaac ttcataggcc
     8821 acgcccagcg tgtaaagcga cggtgagttg gtaatgcgcg cctgatcggt caccgcgaca
     8881 tccgggatgc cgatgtcgcc ggtgtattcc acgccgtttc cgctgtaggc ctcgctcaga
     8941 aagccgggca gtttatgccg gattgcgaaa tccagttcga tatccaccga gttttccaga
     9001 ttctcccgcc agccctgcgc gtccagctcc tgcatgaaaa gcgaaagccc cagcgactgg
     9061 aacgacgagc cgtgccacgt cgccagcgtg tagagatccc ggccgtccgc catccggtac
     9121 ggcttgatct tcattccgga atttttcact gcgtccgccg gcagatcaaa ccgctgcacc
     9181 acaaaacaga gcgggccgcg gaactcgttc acaaaataat tcatgcgtcc ggtaatccat
     9241 ttaccgttgc cgtccgccca gccggtaaag cggtccatcg tcgcattcca gccgaacgcg
     9301 aacgtgccgg actcttcgcc gtaaagatag cggtagcctt ccgcctgccg gtcaataaac
     9361 cgctccagct gcccgcgaag cttaaccgct tcgggaaaat ccttaatctc ggaatgcatc
     9421 agcgcgccga atgccttggc cgctgatgcg gtcaggttgg cgttatcgcc gaaaataatc
     9481 tgcaccgtcc gccggtcaag cagcgccata atccgcgcgc gcagttcatc ggcggcaaac
     9541 ggttccagtt cgccggtgaa aaacttttcg ccgtattccc ctttgcgcgg aatcttggcg
     9601 aatgaaccgt cctgctgcgg aagaatccat ttatgcgctg ccagcgcctg ccagatggca
     9661 tcggcctgct ccgcgccgaa ctcatccaca aaggcctgcc ggttgacttc ttccgccagc
     9721 ggaccgatcc gcttcccgtt ttcaaaaccc agaaaatttg aaagcagacc tttcgcgctg
     9781 agcgccggat cggactggtc ggccagcaga ctcgacaccg tcagcgaaag attgctgaac
     9841 gcctcttcgc gcgaaatgta cggattgtcc gcgtagcccg ccgcgatatt cgccagcagt
     9901 tcgacccatt cgccgatcgc cgtcagctgg gtgaacgacc gcagataaag tccgttctcc
     9961 ggctcgtgat tccatccttc gaccggatag ccggatgcgg catcaatcac gccgttggac
    10021 ggattcacat agcccatcgc attcgccagc agcatctgca tatgcacatg ctccggactc
    10081 accagctgaa aattaaacga tgcaggattt tccggcggtg cacgatgaga acatcctgtg
    10141 cccagcagaa tacccagacc tacggcaaaa attgtttttt tcatcgcgcg ggactttacc
    10201 acggccgccg acagattcag ctcaaattag acaaactcat cagaacgggc tcctggcaag
    10261 gtcgcggcga atgcttttca caatcaggtc gcctcgcggc agacccaaat cagaaggcag
    10321 aatcacctta ttgtcggcca gcgttgcgcc gatgacgctt tcgcaggcct gccggagcat
    10381 ccagcccgcc gcgccggtgt agccgctcca gatcatccgg cccggatcgt gtaccgttag
    10441 atagtcggcg cactgctggt tcggctggcc gccgtagttc tcaatctccg cgccgtgcgt
    10501 gtgcggaatg gctgaaattt tataccagag ccgggcggcc gcatcgcggt aatactgcgc
    10561 cgtcaccggg tcgccctgcg cggcgaagcg ctcggcgagc aggcggcagg cgcgcgtcag
    10621 ccactggacg ccgtgcgaat acatcccgtt ttcgcgcact ccttccggat aggtgctgct
    10681 gcgtccgaga tacggtttgg tattttcgcg cagcggcgga taaccgaggc tgatcgtttt
    10741 ttcgccctcc agaatcttca gcgcggtatc gagcgcaatg cggctgcgct gcggattgac
    10801 gcccgcaaaa accgcccacg ccgcgccgag cgcgtccgtt tcccagtagc ccgcgcccgc
    10861 gatgccgatt tcggtcccgt cgtcgtgaac agcccgcagg tagcggtcgc cgcgccagac
    10921 cttttcaata ttccggccca gtattctcag ctgctgctca tagtaatgcc ggcgtttctt
    10981 cccgcagcgt tctccgatca ccggaagcag atttttcaaa acgcaggtca gaaagaaagc
    11041 catccagacc gattcgccgc ggcccttact gccgatttcg tccagcccgt cgttccagtc
    11101 gccggtgccc atcagcggaa gcccgtggct tcccatccgt ttggtcagca ccagatccac
    11161 cgcgcgcatc acatgcttat aaacagaatc actgcgcggc gaacgcagcg gaaagaaacc
    11221 cataccgtgt ttccccgccg gcagcggcgc cagcggcgtc tcggctttga gatagctgac
    11281 cttttcatcg agcagcgttt tatcgccggt catgcggaca tactcgccga cgccccagcc
    11341 gagccagagc aggttgtcgc tggcgtatga gcggctcagg aatccggtgc gtccgtcctg
    11401 ctggaggaaa aaccagtgtg ccgtatcgcc ttcgggaaac tgctgcgccg cgtgcaggat
    11461 gagctgggcg cgcgccagcg acggatcgac ccagatcata ttgacggtgt cctgcagctg
    11521 atcgcggaag ccgaatgcgc cggaggcctg atagaatcct ttgcgcgccc agatgcgttc
    11581 gctgagcgtc tggtatttca tccacttcac ataggcgtcg aattccggat cgctggtttc
    11641 gatcttcagt gtagcttgca gagccatcca ccactcacgc gtggtttgca gactccgttc
    11701 ggcattttca atcgactgga attttttcac gcagacttcg gcctgcttgc ggctgtcggc
    11761 ttcgccgagc acgatagaga ttgtctgctc gccgcccgcc gggatttcaa ccgtcgtcag
    11821 cagcgccgcc accgggaatt cgtcgcctgc cgccggagcc ggcgcaccgg tctcgaccat
    11881 gaccggacgg ctgaacgagc ggccatcgcc gaaaaagcgg tcgcggcgcg tggcaaactg
    11941 ctcaaccggc gcgctcatgg cggcgaagca ggaaccggcg cggaaacggt tgcgcggatt
    12001 ttcaaaatag agcgcgccgc tgacggcatc gcgcgaaatg aacagcgccc ccgcctgctc
    12061 cgggctgtgc gccagcgcga tctggaaata cggcgcaatg cgcaggcggc gcgtcgcaat
    12121 gccggtatta cggattttca gaatgtaaac gcctgccggt tcatcgggcg gcacatgcac
    12181 ggaaagcgcg gtttccagcc cgcctttttt cattttgaaa accacactgc cgtcgagact
    12241 gaagaagaca tcgtgttcag cggtcttgtc tttgaccgga tcgtagcagg gcgaaaacca
    12301 gtcgccggtt ttctcatcgt agagataaat cgcttcggaa ggaacctgat tgccggtcag
    12361 atcggcccag tcgggagtca gccggttctg ctgggcgtta ccgctggccg atgagtgcag
    12421 accgcggttg gtgaccgcga caatatggcc gagcgcgttg gccatttcgt ggtcgaacgg
    12481 acgcggcgtg aacggtgtca gcacacgcag cgtccggccg tcgtcgagat attccgtgta
    12541 gcgctccggt gtaccgggcg gaatctcgcc gtgtccgatt ttctgcgcgc gctccggctg
    12601 ttttatgcca tgcagcgaac tgtcgacttc cagatccaga tgtttgtaaa tcatttgtgc
    12661 aatgcgcgcc ttatgttcgc agcagccgat caggattttg tgcgaccgct ttccattcgc
    12721 cggaaccggc gcatcgagca gcagcgcgcc gatcgggtcg aacgccgggc aggcgtccat
    12781 gttgagcgct tcgcggaagg ccagcgtttc cagtacacgc ggagcccaga tgtttccggc
    12841 gcggccgata aaatcgacgc ggccgctcag aatgccggca ggctgctttg cagacgccag
    12901 aatgccgtaa tgcttggtgc tgcggtgaaa cgccaccatc gcgttcagtt ccgtgttgta
    12961 cgacatttcc ggatagagcc ggttgtactg cgtatggttg cggtccgcat ccggacggtt
    13021 cagcatccat tcgagatacg gaaccacttt caggttgcgc gctttcccgc tcagatcttc
    13081 cagttcgata ttccacagct cggctccgtc gttgcggccc ggcagccgga tggtgacggt
    13141 aactttaacg ccgttggaaa tgttaattat tttcaaccgg tcgctgtcgc attcataaac
    13201 agacggcgta aaaagttccg ccgggaaatt gccgagaacc ggccacgctt tgttcgccgc
    13261 gccgtcttcg ccggcctcca ccacaaaaag aatccgtccg gacggatcga ccttttcgaa
    13321 tgcacagcgg ctgatgccga atccgttgga agtcatgaag gagttgatct cgccgctctg
    13381 cttcagctcc acttcgtatt tattattgat cagaccgaaa gtctgcccgg tctgcgctgc
    13441 atggcgttcg cgggttttcc ttcgcagtgt aaacagggcc gccacgacag ccgccgccgc
    13501 aaccgcgatg agcagaagcg gaaccgggcc gagaacgtac ccggcctgcc gcgcctggat
    13561 ggcggttccc atactgaaaa cctgcgccca ctgttcgcct ttcggcagat agaacggaat
    13621 cagcagcggc gcccaggtgg taaagcgctt aacgccttcc ggaataaaac gggccgttgc
    13681 ttccgggccg acaaacttcg cgcagcgttc atcgagacgg tcaacgccga ggaacgagag
    13741 gttcaccagc gtcgcaacgc gcagtcccat gtgatcgatc cagatcagaa tctggaacca
    13801 ggcgtaaccg ataacccagc tgaagacggt cagactccac accgtaaaac cgttcgcgtc
    13861 gcgggtcagg ctgttgaaaa tggcaaagaa ggtgcggatc agtccgtcct gattgtacca
    13921 cgccgcttcc gccatcgggc ggaggaaggt gaagatcagc ggcgccatcc agagacccca
    13981 gcgcagaaca taaacggtac cgtcggccag ctccgttacg ccgccgcggg tcgcaatgcg
    14041 ccggattgaa gaaatatcgc gattgagcag cgcggagaga aacgccttgt tgatcgcgaa
    14101 cagcggcgcg gcaatccccc agccgatgac gcccatcagc gcttcgttga aaagaatctt
    14161 cgcgccgccg gaggcggcca gcagtttaat gtgtccccag cggctgatca gcgcggtgaa
    14221 ctcgttcggc gcggtggtgc gcagctgctg acagcgtgcg acgatatccg ccggattcag
    14281 gccgaagctg ttgtagagct caaacttggt tccaatgatc ggcagctgcg ccgcatcgag
    14341 atagaagccg atgcccgcgc cgatgaaggc gcccagtccg ctttcgacaa agtaatagcg
    14401 ccatgatttg acgccgccga taccgttcag accgaacagc aggtcgcgca gaatactcac
    14461 gccgccgaac gccgccgcgc cggccgcgaa gccgaatccg atccgctctg cggtcggcag
    14521 tccggcaaag ttcatgctca tgccggcggc gaataccaga ccgaggacca gaccgcgcag
    14581 ataaagcacc gggtctttat aggcacaggc tgcgcgcccg aaaaacgact ggctgccgtc
    14641 aaaggtttca ataacggttt tgaacagcgg aaaaacggct acgcccgcca cggtcagcag
    14701 cagcgccgga gcggcggcat aaaaattctg tatccacgcc gcactgacca gcgcatgaaa
    14761 cagctgaagc aggcccatca gtacaccgct gaagaccatt ccttttttca gtccgcggac
    14821 ggcatcgtcc tgcgccaccg cgccggacgg cgcgctgccg tgcatcgcgc cgagcaccat
    14881 gccggtcagc agatagacca tcgcccagag cggtccctgt gaaagcatcg tggcgatcac
    14941 agcggcaaag ggccggaaca cggccggcag cgcggccgca acaggccccg atccgagatc
    15001 ggcgatatac ggcgcggcgg tcaccagtat ggaaagtaca aacagagcgc ggtggacttt
    15061 gagggagaaa agcagcttct cgcggttaat catggaagag gcctgcgcga taaactcgcc
    15121 gacgcagaaa atcagcagca cttgcaccgc gaaatactga aacgattgca aagcaagcag
    15181 cagtccgaaa ataaagggcg ccgcggtaat cactgtgccg ccgatcagtc cgagatgctt
    15241 ccggcgcagg ttcaggcggc aaacgttggc gacgtgatag atcaggacaa acagcgccgc
    15301 gccgaaaccg ctgagctgga aaaatccggt cagagaatcc ggcgcctgcc aaagaacttc
    15361 cttgcccgca gcggcggcca gcagtccggg aaacagcgcg ctccgcaacg tttcacccgc
    15421 aaataccgga cgtccggtca gcgtaatctt gagcagcagg ctgatcacga cactggcggc
    15481 ggatacataa aaacagaacc agccggaatc gagcgggttc attttcccga gtgcataaac
    15541 tcccagcacc gaggagataa tcaggacggt ggcgacgccg gacatcagat cccggatgaa
    15601 gcctcggacc ggaattcttt tcttgcgccg gatcacagcg accagcaggc tcagcagcag
    15661 gctgaacacc gtcacgccgc ccagccggcc ccagagcgtg gcacgcaaat tttcccacag
    15721 cggcagcttt tgaaaggtca tgtcgagccg gattcgtccg gcggccggat cggcgccgcc
    15781 ggtccgctga atcagcagaa cggtttcgtt gagcgttccg attttattcc agtcgatcag
    15841 ttcgcgcgtt gtgttccagc ccgggccggg ccgtaccgga aagcgctgcg tgccggttga
    15901 gcctttaatc tcaaccgcca gcgccacatc ggaaagctgc ccctcaggaa tgtagatgcc
    15961 gagttcgacc acatcaacgg aaccggcggc cagcgcggcg ggatattctt tcgcccagac
    16021 gccggccgcg gttccggccg gtatggagta attgataatc agcaggtcgc ggttgatcgg
    16081 agcttcttca aactcgcggg agataaccgc ctgcgccgct ccgatgttga aactgccctg
    16141 ctgcctggcg tcaatgaagc cgaccggtgc cgccggcacc gaagcaaact gaagaagaac
    16201 cgccagtaaa atcccgaccc gcgcgaagct ttttgccata tctaaaccct gcccgctcag
    16261 actgttttat gcaatcactt ccttatcggt ctgccagaat tcggttaatc gcccggtcag
    16321 gatgaagtcg ttgaaaatct ggatgaggcc ctgacgggcg aacgcggtgc ggtaaagctt
    16381 cggcgaatgg tcggggccga agagcgtgta ggcggtttcc ctgataacgc tgttgagcgg
    16441 ttcaaccggc aggttccgaa gaaccgaccc gtcgcctttc gccagccggc acggaacaaa
    16501 cacgttcagt tcaatcgcgt gcaggcggtc ggcgccgacc agttccgccg gtttttcttt
    16561 tccgctccag ccggtatgcg ttgaccagaa acatttcgga aattgggtag ggacggctcg
    16621 ccgagccgtc cgcccttcga cagagatcag gaccggcggc gcgttcggcg aacgcgccct
    16681 accttgaaaa gttccagatg cgacccattg agaaagcgcg gcgagccgcc ggacgggatg
    16741 attgagcgga cggacgcccg ccagcctcca ctcgttttta tgaagcggcg caatctcttc
    16801 ccatttatgc tgttcgcgcc accagcagtc ccacagcgaa cgcagttccg ttttggaggc
    16861 ttgcggccag ccggaaccgg gctgtttcgg aagcaggccg gaaatgccga gcagtacagc
    16921 gtaaacgggt ttccagtcat cgccgtactg cgcgagcgct tcgagcggca gactttgcgc
    16981 cagcttccgg aacggcgttt tgttgtgctt gtagccgagc gcggcggcgg tttcctcgta
    17041 aagcgcctgc gcttcgccgc gctgccggat cagccacgcc atgcgcaggg ttttctgccg
    17101 gaggcgctct tcgccagcgc cttccagtat ccgggaaatt gcgtccggcg acttccggga
    17161 taacggaaat tctgcagcgg cgtgcggctc gtgatacgga taggcggaaa ggtcgaggct
    17221 ttccagatca gcggcgcaaa cgtccgccag cggaatatgc accgtgccgg gcggaaaaag
    17281 cgattcgtcg atttttccgg aaaaccaggt gatgtgaaaa cggacgccgg aataacgcgg
    17341 atcgtttgcg tggccgtggc ttttccagcc gtttgggaaa atatgaattt cggcatcgcc
    17401 ggcgacgcgc cgcttttcgc gcccgatcaa aagcaccgcg ccggaaaagt ccggtccggc
    17461 ttcgagattc cagcgtccgg gattttcgac ggcaaccggt tcgccgtgcg aagttttcag
    17521 attctgcggg cgcaggcgtt catcaaacca gagcgcatgc aggtggcgtt cggtataggg
    17581 aaaccagcgc ccggcggttt cgcaaacggc ttcggcgggc tgctgccggc gataagcggc
    17641 cgccctcggg aacaatgttt ccgaaatgga cggcgcggcg tacatcacgt cagcctccgt
    17701 gccccgcgtt ggggaagtcg aactcggttt cgaagagctc cttgaatttt gcggcggcgg
    17761 cggcttcgtc cggcccttca gcctgaatgg tgatttgcgt gcccagttcg agaccgagaa
    17821 tcatcagctc cagcgcagaa ccgagctggc agcggccaga aggtgcaatg actgtaaccg
    17881 tgcttttgac acctgcaaca gcctgtgtaa tcacggcagt cggccggcag tgaatgccgg
    17941 cgtgattttt aataactgct tttacctcaa ccataacgcg ccagatcatg cgcacggcgg
    18001 cggctttttc aatctttatc tcagaaacac ttgccgcttt gtttttttag acagccgtcc
    18061 ttgttgcagc ggtagcagat ttcgttggcc atcgtttcgc cgcggaaaaa agcgtcgaga
    18121 ttggaaagcg tagtttcggc aatattgcag agcgcctctt tggtgaaaaa ggcctgatgc
    18181 gcagtgacca gcacgttcgg gaaagtaaac agccgcgcca gcacatcgtc ggtcagcact
    18241 tcgccggaac ggtcctcgaa aaagtatccg tcttcttcct catacacatc gagaccggcg
    18301 gaaccgatct ttccggtttt cagcgcatca atcagatcct tcgtctgaat gagtccgccg
    18361 cggctggtgt tgatcagcat cacgccgtcc ttcatctgag ccagactcgc ggcattaatc
    18421 atgtgctgcg tctccggcat cagcggacaa tgcaggctga tgatatccga ctgacggtag
    18481 agttcttcca gcggaacata ttccacgccc gccccggcgg cccagtccgt cttcggaaac
    18541 cggtcgaacg ccagcacgcg catgccgaag cctttgagga tgcccgccag aatctggccg
    18601 atacggccgg tgccgatgat gccggcggtc ttgccgtgca gatcgaagcc gagcagtccg
    18661 ccgagcgcaa aattgctgtc gcgggttcgg ttgtaggctt tgtgcgtctt gcgattaagc
    18721 cccatgatca gcgcgacggc gtgttcggca acggcatacg gcgaataggc cggaacgcgg
    18781 gcgacgtgga tgcgctcata tactgctttg agatcgacgt tgttgtatcc ggcacagcgc
    18841 atcgcgatca cgcgaatacc tttattgaac aggatatccg caaccggcga ggaaagatca
    18901 tcattcacaa acgcgcagac ggcgtcataa cccgccgtca gcggcgcggt atccgccgac
    18961 agccggttgc cgatgtattt gatctcataa ccgtgctttg cgttggccgc gtcaaaaaac
    19021 tcccggtcgt agggtttggc atcaaagaaa atcacttttt tcataatggc ggttcctgtt
    19081 gttcgccttc acaaatacca tttctccggc agactgaaaa atcctttccc gcctaattaa
    19141 gccccgcctg tgcgcttgcc ggaaatagcg gttcatgttt aactgccgcc ctgtttttaa
    19201 ggaactgaac tatgaaattt gcgctgagct ggctgaagga atatgtggat tttgacgaca
    19261 cgatcgcggg gctttccgac aaactgacgt ttgccgggct cgaggtcgaa catatcgaaa
    19321 cgctcggcgg tacgtttgaa ggcgtagttg tcggcgaagt gaaacaaatc gagcctcatc
    19381 cggacgccga caagctgcgc ctctgcaccg tcgagtacgg cgcggccgaa acgctgcgcg
    19441 tcgtctgcgg cgcgccgaac gtcgcggtcg gcggcaaata tccgttcgcc accgtcggta
    19501 cgactctgcc gtgcggcatt aagctcaaga aagccaaaat ccgcggcgtc gaatcgctcg
    19561 gtatgctttg cgccaaggac gaactcggcc tcggcgaaga ccattccgga ctgctggtgc
    19621 tggaggcttc gctcaaaccc ggcacgccgc tcgccgccgt catgggcccg gcggaaaccg
    19681 tcttcgaact cgaaatcaca ccgaaccgcc ccgactgcct cagtattatc ggcatcgccc
    19741 gcgaagtcgc cgccatctac ggcaaaccgc tcaagatgcc tgcgatcgaa ctgaaagaaa
    19801 gcgcggaacc ggtcgaaaaa gaaatcagcg tcgaagtcaa cgactccgtc aaatgcccgc
    19861 gctacaccgc ccgcgtgctg aaagacgccc agatcgggcc gtcgcccgac tggatgaaac
    19921 gccgcctcga agcctgcggc atccggtcga tcaataacgt cgtggacatc accaactacg
    19981 tcctgctcga aaccggccat ccgctgcacg ccttcgacta cacgctgctc aaagacggaa
    20041 agattatcgt ccgccacgcc gccgccggcg aaaaattcac cacgctcgac ggcgtggagc
    20101 gccagctgac cggcgatatg ctggtgatcg ccgacgccgg caaagccgtc gcgctcgccg
    20161 gggtcatggg cggcgcgaac accgaaatca aagacagcac cacaaccatc ctgctggaag
    20221 ccgccacgtt cgagcagtcc ggcatccgcg ccaccgccaa agcactcagc ctgtacaccg
    20281 acagctccta ccgtttccag cgcggcgtca gcgccggcag cgtcgaatgg gccagccgcc
    20341 gcgccgccgc gctgatgagc gaactggccg gcgccagcct ctgcaaaggc gtcgtcgatg
    20401 cttatcccgc gccgaaagcc aaacggcaga tcacgttccg ctggcagtcg gcctgcgaac
    20461 tgatcggcat gaacgtgccg gtcgaaacga tgaagaagat tttccagtcg ctggaaatct
    20521 ccattctctc cagtgattcc gaaaaagcgg tgcttgaaat tccggccttc cgcgacgacc
    20581 tcgaacgcga agtggacatt atcgaagaag tcgcccgcat gaacggcgtc gacaagatcc
    20641 cggaaaaact gccggtcgcc gaaaccgtcg ccggagtaaa cgacaaccgc atccgcgcca
    20701 tcaccgcctg ccgcaaaaac ctcgtcggcc tcggcgcgcg cgaaatcatg aactacacgc
    20761 tggtcagcca tccgctgctc gacctcttcg gcgccagcga ccgcgctgcc cgcgaagagc
    20821 tgccgcatcc gatcagcgcc gaacagtccg tcctgcgcac ctcgctgatt ccgcagatga
    20881 tcgagagcct cggccgcaac aagtcgcgcc agatcgacga agcggtcttt ttnnnnnnnn
    20941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgggccgt gccgctgcgg tgttttccgg
    21061 caaagaaaaa atcggcgtac tcggtctgat caattccgga gcgcgcaaag agtggcgcct
    21121 gaacgatccg gtcgccgccg ccgagctgaa cctcgaaccg ctgattgcca acgtctggaa
    21181 acgcagcgaa gtaaaagaca ttccgctcta cccggccatc gaacgcgact tcgcgtttgt
    21241 tgtggatgaa gcaatccgcc acgaagatat tctggctgcc gtgcaatccg ccgcgcccgc
    21301 cgagctcgaa agcgttaagc tgttcgatat cttccgcggc aaagcgctgg agaagggcaa
    21361 aaagagcgtc gcctacagct tcgtctaccg ctcgccggcc ggcacgctga ccgacgaaaa
    21421 ggtcaacgcg ttccacgaag ccgtcggtaa gaaagtttgc gctgcggttg cggcggttat
    21481 tcgcgaagga tgaccccttg acggccaggg cagtcagcag taccatgcag atgtgaggcg
    21541 gagagagaac gaaatcctca cactgttctt taccaattta agcccgcccg gcgaggcaac
    21601 aagcttccga tccgttaaca aaataaataa gggcgctcag gataagtgct gatgtattcc
    21661 atcttcactt tagggtgccc accttgtaaa tcagggttcg gaaatgagcc tcaccggcac
    21721 gggctctttt taaagccgct tgggtattaa cctgagcggt tttttatttt aaccgctaat
    21781 gaacgcgaat taacgctaat gggatgagtc aaaattagtg tccattagtg gaaattagtg
    21841 gttaactttc tcgcatggaa ctttttgagg aaaaaccgtc cgcaagcctg aagcccggcg
    21901 caggaaccgc cccgctggcg gcacggatgc gtccgcgtac gctggatgaa atcgccggac
    21961 aggagcatat cctcggcgcc ggaaagctgc tgcgccgcgc gattgaggcc gaccgcattt
    22021 cgagtgtgat tctctacggc ccgcccggct gcggaaaaac ttcgctggcc gaggtgattg
    22081 cgctggcgac cgaccgccgg ttcgagcgca ccagcggcgt gctggccaac gtcgcgattc
    22141 tgcgcaagct gctggaacag gccgccggct ggaaaaaaat ccataagcag gacacgatcc
    22201 ttttcatcga cgagattcac cgcttcaaca agtcccagca ggatgtgctg ctgccgtacg
    22261 tcgaaagcgg cgacattatt ctgatcggcg cgacgacgca taatccgttc tttttcatca
    22321 acacgccgct caattcgcgg tcgcagattt ttcagctcaa tccgctcagc gaagaggcca
    22381 tcaagacggt gctgaagcgg gcgatggagc atccggcagg actcaacgga gcggtcacgg
    22441 ccgacgacga cgcgctggcg catctggcga cggtttgcga aggcgacgcg aggcgcgcac
    22501 tgaacgcgct ggagatcgcc gcgctgacga ccccgccggg cgaagacggt atcgttcatt
    22561 tgacgcgcca cgagacggaa gagtcggttc agaagaaggc ggtgtcgtat gatcacgatg
    22621 aggacgagca ttacgacacg atctcggcct ttatcaaaag cgtgcgcggc tccgatccga
    22681 acgcggcggt ttactggctg gcgaagatga tttatgccgg ggaggacccg cgttttatcg
    22741 cgcggcggct ggtgattctc gcttccgagg atatcggcaa cgcggacccg cgcggtctga
    22801 cgattgcgac ggcagcggtt caggcggtgg attttgtagg catgccggaa gcgcggatta
    22861 ttctggcgca ggccaccacc tatctggcct gcgcaccgaa aagcaacgcc agttatctgg
    22921 cgatcgacgc cgcgctggcc gatgtggaaa acggccggac gctgccggtt ccggatcatc
    22981 tcaaaaatgc gccgcacccc ggcaagggcg aggtgcagta ccaatatgcg catgacggca
    23041 aggatcattt tgttgatcag gaatatgtgc cgacggaccg gatttattac gcaccgaccg
    23101 atcagggcta tgaagagact ctgagaaagc ggctggaata ctgggcctcg agacggaaga
    23161 aaaaataaaa ggcgcagtta ttttggagtg cggcggcttg acgccgcttt tctatgtctt
    23221 accctgagca catgaaacag tggccacacg ccccgcttca catactcgaa gaacgaggaa
    23281 cctacatggt gacttgcggc acctatcaga aatgtcatca ccttaacgaa gcttcgcggc
    23341 tcacattttt ccaaaatgcc ctatttgaaa tcgctgaaga atttggctgg cggctgcagg
    23401 cctgggccgt gctttccaac cattatcacc ttatcggctc atccccggaa catcccggaa
    23461 cgcttcaaaa aatgctgtct aaactccata ccgtcacggc catacaactg aacaaagaag
    23521 ataacgagcc cgggcgtaag gtttggtacc agtatttcga cagccgcatc acctatcaaa
    23581 actcatattt ttcccggtta aagtatgtgc atcaaaaccc ggttcaccac ggcgtcgttc
    23641 ataacgcgga aacctaccct tggtgttccg ccgggtggtt tgcccggtcc gccggcagcg
    23701 cgttttataa aaccatacaa ggtttcaaaa cagatagtgt aaatgtaacc gatccatacg
    23761 atacgatccc gatcaacttg gaaattgaaa gcggcgtcaa gccgccgcac tccaaacagg
    23821 aatgcccgtc atgacgaaag cggaaatacg cgcgcggatt gccgcacaaa aaaaaaccgt
    23881 tcaggggctg gaagcgccga gtgcggcggc cgtcaaaaac ctccagtatc tggccgcctt
    23941 ccagtcggcc cggacggttg gcgcgtacat gccgctgccc ggtgaagttg atatctcgcc
    24001 gctttttgcc tgcccggaaa aaatattttt cattccggcc ttcgacgaat cgtcgggcct
    24061 ctaccggatg gcgcggctgg ccggagaact caagacgggg cgcttcggca tccgggaacc
    24121 ggcagttccg gtgttcgcgg aggaacacga tctcgatctg attatcgtac cgggagtcgc
    24181 ctttgaccgc gccgggcaaa ggatcggccg cggcggagga ttttacgacc ggctgcttcc
    24241 gcagtaccgc gcgatacgca ccggaatctg cttcgatttt caatgtctgg aagttattcc
    24301 ggcggaagac catgacgtcc ggatggattt tacagtgact gaatcgaaaa ttcttaaatt
    24361 tgcgatgaac agttagacaa gctcgtccgt cagagatatg cttaacagag aaaaataaat
    24421 tttgaaacaa aaggtaattt tatggacagc ataggactgt ggtggggtga catgcagaac
    24481 ttcgttctca gcatcgggtt cgtcgttctg ggcttctttt tccacgctta tctgacccgc
    24541 accaatgcgg tagccgccag ccatcgcgga tcagcgatca tggatgatgc ccgcaaacag
    24601 gcggaagcga tcaaacatga agccgatgtc agcgcccgcg aagagattct caaagcacgt
    24661 gaacgggccg aaaaagagca ggaaaccaaa cgccagagta tcatcgcact cgaagaccgt
    24721 gtcgtcgccc gcgaaaaaga catctcccgc aaacttgaac tgctcgaagc gaaagaccgt
    24781 acgctggccg aaaaacttga taaagctgag cagcagagcg aagaactcaa ggcccagcaa
    24841 aagacgctca aggatctcat tgaagcggaa acccgcaaaa ttcaggaagt cgccagccta
    24901 tccaaagaag atgcgcgcaa gctgatcatg gatcagatgg aaaaagagct ggaaggcgaa
    24961 atcagcggac tgatccgcca ccagcagaag gaaatctacg agcgggccag agcagaggcc
    25021 cgcgatatcg tctgcacagc cattgagcgc tacgccggcg aacacgtcag caacatcacc
    25081 gtcacacagg ttgccctgcc ctctgaagac atgaaaggcc gcctgatcgg caaggaaggc
    25141 cgcaacttta agtcgttcga gacggaatcg ggcgtcaacc tgatcattga tgaaactccc
    25201 ggcatcgtac agctttccag ctttgatcct gtgcgtcgcg aagtcgcccg cgtggcgctt
    25261 gaaaagctca tgctcgacgg ccggattcac cccgccagca tcgaagagac gatcaaaaaa
    25321 acccgttctg aaattgacga aatcatccgg cagtccggtg aagacgcgct ctataaactc
    25381 ggcatttccg gcgttgcgcc cgagctggtc aaagtccttg gccgcctgaa ataccggacc
    25441 agctataaac agaacgtgct cgatcactca atggaaatgg cttcattgat gggtatgatg
    25501 gctgcggacc tcgggctgga cgttgcactg gcgaaacgcg tcgggatatt ccacgacctg
    25561 ggcaaagcgg ttgaccatga ggttgaaggc tcccacgcca tcatcggcgc caacctgctg
    25621 cgcaaatacg gcgaggttcc gctggtctac aacgccgtcg ctgcgcatca cgaagacgtc
    25681 gaaaagagca gtgtttacgc tgtactggcc gccgccgccg atgcgataac cgccgcccgt
    25741 cccggcgcgc gcatggacac caccgatctc tacttccagc gtctcgacaa actggaaaaa
    25801 atcgccatgt cgttcaaagg cgtcgataaa tgctacgcca ttcaggccgg acgcgaactg
    25861 cgcgtgatgg tggaaggcgc caaagtggac gatgcccgcg cgatgattct tgcccgcgaa
    25921 atcgccaacc gcatccagga tgaagtcaaa tatcccgggc aggtcaaggt gacggtgatc
    25981 cgggaaaccc gggctatcga atacgccaaa taagaggccg cctcaccacc agatgcggca
    26041 gccgccttcc agcgtgaagg cgttttgaat gtgcctgatt tccggttttt cgccgaccac
    26101 ttcgatgaca tcgaaacgga tatacggcgg gcgcagttta cgtttcttca ggaaatgaat
    26161 ggcggcgcgc gacagtttgc ggcgcttggc gcgattgacc gccgtaaacg gccgtccaaa
    26221 atcttcattt gcgcgggttt tgacttcgac aaaaatcaac gtgtcgccct gctgtgcaat
    26281 caggtcgagc tcatccgcgc cgatccgcac attgcgagcg atgattttca gtccggcttt
    26341 cttcaggaat ttttccgcct gatcttcacc gcgccgccca gtgcgcaaat gcggcgcctc
    26401 ttttttaaac cattgcagca gccgaatcac agattcaact ccccttggct cagctcagaa
    26461 accgggcgga acgttctgcg gtggtgcggc gaaggcccgt ggcggcggat ggcggcgaga
    26521 tgctcggcgg tgccgtagcc tttgtgtttt gcaaagccgt attgaggaaa ctccgcatcc
    26581 agcccgagca tcagccggtc gcgggtgact ttggcgataa tgctggcggc ggcaattgaa
    26641 agactctggg catcgccttt gacgatgctt tgcgatttgc agcagagccc gcgaaccggc
    26701 cgtccgtcga ccagcgccag atcgggctga gggctgattt gcgccacggc atccgccatc
    26761 cctctccagg tggcctgcag aatgttaatc tcgtcgatga cgtccgggcc gatgaccgca
    26821 caaccgatgc gggcaaaact gcacgcctgc agctgctcga aaaaaacttc gcgctgtttt
    26881 gccgtaagcg ctttcgagtc ggtgagcccg gtgaaggctg ctgcgccgcc gttttcaagc
    26941 ggctcgcggc tgaaagcaac cgcggccgcc acgaccggac cggccagcgg cccgcgcccg
    27001 gcttcatcca caccggcaat aaaaaagagg cctgattgcc aggcctcttt ctcataacgg
    27061 agcatgtcca tcgcagatta cacacggcgt tctttaatgc gtgctttctt gccggagatg
    27121 tcgcgcagat agaacagttt cgcacggcgg gtctgaccgc tgcgcacaac ttcgatcttc
    27181 gctacgttcg ggctctgcat cgggaatacg cgttcgatgc cttcgccgaa agctacacgg
    27241 cgaacggtga aggtttccgc aacgccagca ccttcacggc cgatcaccgt tccggtgtac
    27301 agctgaatgc gttcttttgt gccttctttg attttcacat gcacattaac ggtatcaccg
    27361 acattgaatg cggggatttc ttttgtgcac tgctctttgc caatttttaa gactaccgga
    27421 ttcataattg ttctctttct tccaattcca aaagcgagcg tcgaataata cccaactgcg
    27481 tctttcggtc aagcgccatc tctattttcc gccccgcagc ctgaaagcag atccgggcgg
    27541 cgttccgcag tccgcttttc agcctgccgg cggcgccact tcgcaattgc ggcatggtcg
    27601 ccggaaacca gttcctccgg cactttcatg ccgcggaatt caggcggacg ggtgtactgg
    27661 ggatactcca gcagcggttc gctgaacgat tcctgttcgg cggcgccctc gccgcccagc
    27721 acaccgggaa tcagccggac cacggcgtcg atcactacag ccgccggcag cactccgttg
    27781 gtcagaacat agtcgccgat ggatatttct tcatcaatca gcgcttcgcg aacgcgctca
    27841 tccactcctt catagtggcc gcagacaaaa atcagatgct cctcgtttga aagcgtctgc
    27901 gcccgccgct gcgtaaacgg ctttccctgc ggacacatca gaataacgcg ggatctttct
    27961 gtacggattg attccactgc actgaagatc ggttccggct tcatgaccat accggggccg
    28021 ccgccgaacg ggcggtcgtc ggtggtacgg tgtttgtctt ccgcaaaatc gcgcaggttg
    28081 atgcagttaa acgtaacgtg ccccgctttg gcggcacgcc gcatcatgct ctgaccgaaa
    28141 aagccggcga gcatttcagg aaagatggtg attacgtcga tctgcataac aaaaaaaagc
    28201 ggcctttcgg ccgcttttgt atcacggggt gattttttat tccacaactt cgagcatcgc
    28261 gcggcggccg tcgcgggcag cgatcgctcc gaggagcgta cgcatggcgc caacggtttt
    28321 tccgctgcgt ccgattactt tgccgacatc tttttcatgg cagcgcagtt cgtaaacaat
    28381 gcttttatca cctttgactt cggtgatctt gacgtcgcgg ggatggtcaa ccagagcggc
    28441 gaccatctgc ttcacaagtt tcttcatgtt taggccccct gcgattcgga agctttttga
    28501 tcagctgcgc cgaccgggac gccttgaccg gcttcagcct ttttcaggag gctggcggcg
    28561 gtgttggaaa tctgtgcgcc ggtacccatc cagtgtttga cccgttcaac attgacgctg
    28621 aaattgtcac ctttttgctt gggatcgtac cagccgagag cttcgaggaa gcggccggtc
    28681 gtcgcgaagc gggaatcggc taccacaatg cgataaaacg gttttttgat ggtgcccatt
    28741 cgacgtaaac gtatttttgt tgccataatt tctgtaatct ccagtccagg ttgcgggctt
    28801 tcgtccgcgg aaaaagaggc tactgtctat atcatttgac acccctcatc aacctttttt
    28861 ccgacttttt caccccgcgg tccggttctg cagtttttcc accacctgca gatcggcctt
    28921 cgaccaggcg tatttgctga gttcagaagc ttttacccag gcataatcgg cacaatgaat
    28981 cggacgggga cgccctgaaa tgaccgttgc gaaatagaca tgcatggtca tggtgaaatg
    29041 actataggtg tgcttcaccg tcaaaaagaa ctcaccgact gccgtgttaa tgcctagctc
    29101 ttcttttaat tcccgggcga tgcactcctg aattgtctct ccggactcct gcttgccgcc
    29161 gggaaattcc cacagtccgc cgagcatatc attctgccgg cgctgggcga tcagcacctc
    29221 gcccttccgg ttcaaggtca cggccgcgcc gaccgtaata tgagggacct gcggcttttt
    29281 ctttgttaca ggaaactttt ccggcgcgcc gcccgcccag gcaaagcaaa cgctctgcat
    29341 cgggcattcc ggacattttg gatttttcgg cagacagacc agcgcgccga gctccatcat
    29401 cgcttcgtta aattcaccgg cgcggccttt caccagcaga tcgtccgccc gggcctgcat
    29461 ctgtttttta cctttggcgt cacgggtgtc gccgccgaag gcgaaaagcc ggcttaaaac
    29521 gcgaatgacg tttccgtcca cgaccgccgc gtcggtgttg aacgccaggc tggcgatcgc
    29581 cgccgccgtg tacggcccga ttcccggcat ctttgccaat tccgcaaccg cggccggcgg
    29641 tttcccggcg tattcaccgg caatagtttt tgctgcggcg tggaggttgc gggcgcggct
    29701 gtaatagccg agcccttccc agcatttcag cacatcgctt tgcggcgcgg cggcgagcgc
    29761 cttccacgac ggaaacgttt tcatccagcg gcggtagtaa tcgatcaccg tgtccacgcg
    29821 cgtctgctgg agcatgattt ccgaaaccca gacgcggtac ggcgtacggt tccgccgcca
    29881 cggcaggtcg cgcgcattcg ccgcaaacca cggtaaaagg ctgttccgga cggcctgccg
    29941 gacttccggg agcctgtctg ttttcatggg ttgtgatttg actgcctgca tggttggact
    30001 agaatccctg caaacattgg gagttgcaac catgattacc aagagaatgt cagcagctcc
    30061 cgacacagac gaatgaggtt ctattatgtg taaaaaactg gttctgctga ccgttgtgct
    30121 gctggcctcc ctgaccaccg tacaggccgc atggaaaagc aacatcacca tgctggtggt
    30181 gccggaagag aaaatcccgc tccagattgc acaggatctt tcccggcgct atcccgttct
    30241 gctggtgagc taccggctgg cgcgcggcga agcaaaactc cacgcctgga acggcgatac
    30301 ctggattccg gtatcggcag aagattacgc caacggcacc tttttcgcca accgcccaag
    30361 ccacgccatc atcgtggaaa acgaacgggt tcacgtgccg ctcgcactga cgccgaacag
    30421 tacctggtgt accaccgcca gccggctgac caccaccgac cctcgcatcc tgctccacct
    30481 gtttggacgt tactttaatt tccccttccg ctactgggat cagtttgccc agcgctacag
    30541 ctacaccatc gaagagatca acccgacact ggaaaacgtg tactggtgga accgttacag
    30601 ccgcacaaaa gataaaaaca gcgcacgcct cgctttggcc gcagatatga ataaatggac
    30661 ccctatcgct ctgctgccgc cgccgcgcat cgaaccggta acactcgaaa aagaacctgt
    30721 cattcccgaa gttgaaaaac ccgcgacaga accggtcgac ggagccgttg atatcacagt
    30781 cagcgcacct gaaccgaagg ttgaaaaaaa gccggatact aaaccggtca tcaaaccgga
    30841 aatgccggca tccaagccgt tcccttcact caaaccggaa ccggtcatta aaccggctgc
    30901 ggcagccgaa aaagctccgg ccggtgaacc ggaaccggtt attgaatctg tccccgcgat
    30961 tgaaccggcg ccggcacctt tagtggaagc ccctgcaacc gccccggcgg ctgaaattga
    31021 aaagacagtc aacgcggaag tccccgcgcc ggcaccgctc gtaactgaag tcgatccgtt
    31081 tgcagcagat gaagttcctg cggccgaaat tgttgttccg cagcccccga aaaaaccctg
    31141 gtggaaattt taatgcataa attattcatt ctgctgaccg ctctcattct ggccggatgc
    31201 accacatcgc ctgcgccgtt tgaatttccc gtttccccgc gcctggcgac ttcgtataca
    31261 aacggtgaaa ccattatgac ctggaaagcg ctcagcaacc agacctacac cgtctactac
    31321 acagacgccc ccctcggcaa actggcagac tggaaacctc tgcctcaggc aaacaaactg
    31381 agcggcagcg ggaaacagat ttctgttaca gacaaaacgg gggccgatgt gccgcgacgt
    31441 tatctgctgc tgaccggcga ccagaaaccg tattaataac tacgagggtg atgcttccgg
    31501 cttatccgcc tctgcattaa tcagcagaaa ctccagctga cggatttttt ccactgtacc
    31561 gagcaccagc agttcatcgc cggcatacag atgttccgcc ggacccggcg ttacaatccg
    31621 ctttccggcc cggcgaatgg ctgcgatctg gactcctgtc tgacgcgcaa tattccactc
    31681 cgccagtgtg cgatcagcaa agctgacagc ctccggaacc gtcagagtat ccagccggat
    31741 ttcatccagc cgttcagact ccgccataca ggtactgtta agaaatgcgc gggctgccgg
    31801 aatctgcgac ggctcgccga gaagaagaag cacatcctga ggaaaaacag taaaccccga
    31861 ccccggggca tcaatgacca taccctgccg ttcgactcca agaaccgaac atttgaaccg
    31921 gttcggcaga tcaagctgag caagcgaacg cccccggcaa agagaagatt gcggaatcga
    31981 aacctcttcg atgttcagat cccagtcgct gtcatgccgt aaaatcagcg ctgcgggaac
    32041 ttgctgcccg gccgcggcat ggcgctgcag ccggttttcc gctacgctgt gaagatgcgc
    32101 catcggcttc cagagcaggg ccccgctgag aacaatgacc gacgctattc cgataaacgt
    32161 ccagaatccg ctgagcgcaa acggcagcag aagccacagc caggcggaaa gcaaaagcag
    32221 cgaaacagtc cgcaaagcct gctcaatcgt gtgggccgca gcgcgtgttt cccggcgggt
    32281 atatcgccgg gccgccactt ccgaatgaag catcgtcagc acctctatgt tgcgccagac
    32341 ggcaatcagc acaatcaaaa caatcagccc gaacggcccc cagaaaacca ggttaaacac
    32401 ccaccgcatc cagttctgtc cggatgcaac cccgtcaaac atcgcatgca gtttcggcga
    32461 aaaaaccatc agccccgtga cgaacagcat ttcaactgca atccgcagaa gacatttacg
    32521 ggagatcttc cagaccatat ccgacgaccg ccgggccgcc agccggttga gcccgttctg
    32581 ataggcggcc agcgcctgag aaaaccagcg gggctgacag gcgaccagac catccgccag
    32641 ccgaccgctc acaggcagca gcaccgacga cgccatcgtg gaaagaatag acaccccgac
    32701 aattacagcc ccgaacgttt ccggcagccg cccggcgcca acaccgatct gagcgaccag
    32761 aaatgaaaat tcgccgatcg gaatcagcac cagccccgtc gtcaccgcat cgcgcagatt
    32821 acgccccgcc gccagctgac cgacggtgca aatcgccgaa cgtccgaata ccgccagagc
    32881 ggtaaacagc agcaccagcg gccaggccgt aaaaatatgc cgtatgtcaa tcaacatgcc
    32941 gatcgacaca aaaaacaccg ctccgaaaag atcgctcagt ccggaaaaag ccttttcaat
    33001 ctgcggcctg tccggcgttc cggccaccgc catccccagc acgaaggccc cgagcgccaa
    33061 tgaatacccg gcactccggg cggcaaccgc cgcgccgacc agaatcgcgg caagaatcac
    33121 attgcgcagt tctggccgga cggattgctg cagcagatgc agaatacggg gaatgaacag
    33181 caaagccaga attgcaagca gtacaacaaa agaggcgaat gcccccagct gctgcagcac
    33241 cggcaacgac acatccggac cgtcatgcag ctgggagctg agcaggctga gcagaatgat
    33301 gacgttaaaa tcctccagca gagtctggaa catggccgcc tgcccggtcg cccggtgaaa
    33361 ccggcccgat tcatgcagca gccggccgat aatgaccgag ctggaagtga taaacattcc
    33421 cgccagcagc agtccgccga ccttgcccag cccgagcata tgcccgattc cccggcaaag
    33481 ggtaaacagt aacgccgtgc tgacagccgt cgcggtcagc gatgcgatcc cgctgttccg
    33541 gatctgtcta attccaagat tctggccgat cgagaacatc agaaaaatca ggccgacctg
    33601 cgaaagcaga cgcagactct ccgaatcaga aaccagcgtg acaaccccgg cggggccgag
    33661 cagcaccccg gccagcagat agccggatac gaccgacagc cggatgcgct ggcacagcca
    33721 gcccgcgatg ccggccacag caagaacaac cgctaaatcc tggaggaatt ttaactcatg
    33781 cataattgaa ttcctgatat atctggaacc gttggcgccc cgtgtggtaa acagctccgc
    33841 aaaatataca aacggacatt ccttcttaaa aggttatttc atgcgcctga aaaaaactgc
    33901 cctattgtta atcgccggct tactgactgt ttgtactgcg atacccgcct ttgccggcgt
    33961 tcgcaccgtc acctcgcgcg gaaaaaacta tgtcaccctg cccaacctcg catcctacta
    34021 cggaatgacc atctcccagc cgtccggcaa acggatccgc ctgcagaaca aattcaaccg
    34081 gatcgaattt gaaaccgacg agcgcagcgt ctggatcaat ggaacccttg tcgcgctcag
    34141 cgaaccggtc cgcaaagtcg gctggtcgtg ggccatnnnn nnnnnnnnnn nnnnnnnnnn
    34201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34261 nnnnnnnnnn nnnnnnggca accggtacga cgccgccaac atggcactca gctaccgcat
    34321 ccagcagaac ctcatccgcg ccaccggaca ggaagatcgc ggcgtcaaac gcgcccgctt
    34381 tgaagttttg cgcgacgcgc cctgccccgc cacgctcgtt gaaatcgcct ttatgagcaa
    34441 cccgaaagac gaagccatcg tcatggatcc ggccggacgc gaccggatcg cccgcggcat
    34501 cgccgacggc atcgtcgcct acctgactga cgcccgccgc tccaaaacca aataaaacct
    34561 acaacaccgc cgtgcggacc gactcgccga tcagcaccag cgtcgccgaa accgccgcgc
    34621 cgggatgcag cttctcgacc gcttctttgt acgccgccag ctgcggtgca taaacgttca
    34681 gcgcgttctc cggcaactcg ccgctgaatt tatagtcgaa gattcgaaac cggtccggaa
    34741 actgcaccag cagatccatc gttccgtcaa gaatcacatc gccgcgcttc agcacaaacg
    34801 gatactcggc cagcagcgcc gccgcacccg ccgtctcccg gcgcaagagt tcgcgcgccg
    34861 cttcgagctg ccggataagc cggccggact ccaccgcgcc gaactcccgg ctgaacatct
    34921 caaccagcgc cgggatatcg ccattccact tgtttttcgc cagctcctcg agaactgtat
    34981 ggccgagcga gccgaacgct ctgcgatctt cggccggctg cgggctttgg gttttgggtt
    35041 ttagggcttg aaccaaactc gtcaccgttc ggcgctcgag accttttgga atttgcaaag
    35101 gttggaattc aatctcttgt ctaaagccta aagcctcagg cctaacgcct tcactttcag
    35161 gaatctcatc aaaccgcaac ctccgcaaaa gatcgggact cgcgctttgc aaaaacgctt
    35221 cgcttaactt cagccagccc ttcggctctt gcgtgccggc gccggagagg acgaccaggt
    35281 cgcgggcgcg ggtcatcgcc acataaaaca cattagtcag ctccagctcg cgcatcgttt
    35341 tatcggctgc gcgcgcggcc tcccagcccg gcgacagcac ttcttcgccc gcgaggccgg
    35401 acagcttcat ctccaggcgg ccttccggcg aaaacatcgc gaagcccggc tcgctgtttt
    35461 ctccgaagct gatatcggga acaaagcaga cgcgctgcgt cagccccttc gcgccgtgta
    35521 ccgtcatcag cgtcaccgca ttctgctccg gatcgggcag cagcgcttcg gcggcaccgc
    35581 tgcgcggcgg cgtttggata tatttttcaa accgccgcac cacatccggc agcagcacct
    35641 gcccgccgcg ttcggcggcg cggatccagt cgagcgcctt tttaaaattc gccagccgct
    35701 gcccgccgcc cggcagaccg gccagctgcg cgtcgaaggc ggtttcgcgg acgatgtcgc
    35761 ggatcagttg cgacgccagc ttttgcccgc tcagcgcgcg gtagcgcagc agccgctccg
    35821 ccgcctcgga accgctctcg aaaaactgcc ggcgcagaat ttcgtgatcg aacgtttcgc
    35881 cgtcccagcc gagccggaca atggtttcgt ccggcacatt ggcaaacggc gagcgcagga
    35941 atccgaccag cgccagatca ttcgccggct cgcaaagcgt ttgcagaaac agaagcaggt
    36001 cgcgcacttc ctgctgctcg aacagcgcgc ggcctttgcc gccgctggtg tacggaatac
    36061 ccgcatcgcg caacgccttt tccagcaccg actgatgcgt cgagcgtttc agcagaatca
    36121 gaatgtcgcc gtaacggaac ggctcgcctt cggcaaacgt ccggccgtta tgtccgaact
    36181 tcgggcgcca ttccgcgccg ccgtccacca gcagccggat gcgccgcgcg accgccgcca
    36241 tttcatgctc ggtcgtactc tcgtcgccgt cctgcatcag aaactcgacg cacggttttt
    36301 cgccggactg aacaccttgc ggttccagcg cgtcgtaccg ttcgtaaatt ccgctgaaca
    36361 gcgcgttgac cgcgctaatg acccggtcat tgctgcggaa gcttgttttc aaatcaatcc
    36421 gccggacgcc gccgcgcgcg gtcattgccg cttcgagatc cggcatcacc tgcggatcgg
    36481 cgctgcgcca ggcatagatg gactgcttgc ggtcgccgca gaccaccaga cgggtttctt
    36541 ttccccagag cgcttcaatc acttcgcact gcacccgcga ggtatcctgc acttcgtcca
    36601 cgatgatcca gtcgaagcgt ccggaaaaat cgccgcgccg gagcagatcg cgcgccagca
    36661 gaagctggtc gccgaaatcg accgcgtcgc gcgatcgctt cgcggcgtca aagccgcgcg
    36721 cgcaggccga caggcaccgt gcaaacgcgc ggaactttcc ggcgttctgt tcgtagaaat
    36781 actcggtcag aaaccgttct ttgagttccg ccagctcatc ggaaaggtgc ttcacctttt
    36841 tggcctgacc gaatttcgga atcgccgcga tccgcgccgg attcggaaag tcggcgagcg
    36901 ctgctttcag cgcgtccagc gcgtcggcgg cggttttcgg cagatcaaac gtttcgagtt
    36961 cggcacagat gcgctcaaag ctctgcttgc ggacggtgcg ttccggtacc ggaagcatcg
    37021 cctcggcgtt acacagatca agtccgcgac cggcggcctt ttcgagcagg gcgcggacgg
    37081 tattgatcag gctgtacgaa ttatccggcg cgccgagaat tgcaacgccg gcgcagaatt
    37141 cgcggaagtc tgcatccgcc tccagcgatt taaaaatttc ggctttcagc acctcgtcga
    37201 gcagcgcgtc gcgctcttcg tcgtcgagcg ttttgaacgc cggagaaagt ccgagctcca
    37261 gcgcgtgttc gcggagcagt ccggcgcaga acgcgtgaat ggttgaaatg tacgcggcgg
    37321 gcagcgaacg caaaacccgc cgcgcgcggt cggccagctc atcgtcgctt ccgagcgcgg
    37381 acttctctac ggagagcatc ttgcctgaaa cacggctgcg cagttcggcg gcggcgttgt
    37441 cggtgaaggt catcagcaga attttttcgg gcgcgaccgg cgggcggccc ggcgggagct
    37501 gctcatgatt tcggcccagc accagcgcgg aatagagctc ggtgacctgc caggtcttgc
    37561 ccgttcccgc cgatgcattg aaaatcatat tcatggccgc gaagtatgcg gagaaagccg
    37621 gccgattgga atgtcggaat cgcggaatat tggcagcccg gcaccgcccg gccggactaa
    37681 ccattgacaa tcaccggcgg cttgcgcatc ctgccgaatc cttcacataa ccgacgaggc
    37741 cttacatgag aaacccgcac gacgtgaaca gccgcaaaac gctcaaagca ctcagcgccg
    37801 gcacggcact gctgaccctg ccgctgctgc tgccggccga cgagccggtc cgcccgccca
    37861 acattatcta cattctggcg gacgacctgg gctacggcga cctgagctgc tacgggcaga
    37921 aaaaattttc cacgccgcag atcgaccggc tggcccaaga cggcctgcgc tttacgcagt
    37981 tctatgccgg cagcgccgtc tgcgctccgt cgcgctgctc gctgatgacg ggcctgcata
    38041 ccggccacgc gccggtccgc gccaaccggg aagcaaaatc cgaaggccag gcgccgatgc
    38101 ccgagggcac agtgacgctg ccggcgctgc tgcggcaggc cggctatgtc agcggcatgt
    38161 tcggcaagtg gggcctgggc gcaccgggct cctccagcga cccgatggtc tacttcgacg
    38221 agtttttcgg ctacaactgt cagcggcagg cccactccta ttacccggat catctgtggc
    38281 acaaccgcga aacagttccg ctcgacaaaa agacctattc gcacgacctg attctcaagg
    38341 cttcgttcga tttcatccgg gcccaccgga acgaaccctt cttctgctac ctggcggtca
    38401 ccattcccca cgccgccatg caggcgcccc ccgcattgca tgagaaatac cgcaagctgt
    38461 tcccgcagtt tgaaaacctg atcggcaaat acgatggcgc gcaggttcag aatcccgtcg
    38521 ccgccctccc cgccatgatc gaacatctgg ataccggcgt cggacagctg atggcgctgc
    38581 tgaacgaact cggcatcgac cggaacacgc tgatcatctt caccagcgac aacggcccgc
    38641 acaaagaagg cggcaacaat ccggcgttct ggaacagcag cggatcgctg cggggcggca
    38701 aggccgacct ctacgaaggc ggcctgcgcg agccgttcat cgcgcgctgg cccggcgtta
    38761 tcgcaccggg cacggtgtcg gaccttccgg cggcggcctg ggatattctg ccgacgctgt
    38821 gcgaactcgc ccggacggct ccgccggcgg acatcgacgg catctccctc gtgccggcgc
    38881 tgaaaggaca gccgcagccc gcgcacgatt acctctactg ggaacttgag cagcagggcg
    38941 gccagcaggc gctccgcaaa ggcaactgga aagcggtgcg ccggaacatc ctcaaaaacc
    39001 cgcaggcggc gatcgaactc tacgacctgt caaaagaccc ggcagagacc accaacgtcg
    39061 cggcccttca tccggaaatc gtccaaaccc tgaccaccct actgcaacag gcccggaccg
    39121 aatcgaaaac ctttccgctg ttccccgcgc cgaaaccctg accgccgggc cgcgggcgac
    39181 tggaataccg gctgaagagt tgacagctcc gcgccgcgtt ttcacactcg ccggaactca
    39241 acttactgac cgccttcggc ttcggcgtac tcggcacctt ctggtttgcc tggcgaggat
    39301 catctgtcgc atctgaaaaa cagcttgaga aaggtgtcca ctgatcttca ctaatcagca
    39361 ctaatgtgtt tcgcacctct aaaatcagtg tcgattagtg aaaattagtg gttaacatat
    39421 cagcatgaaa ttcgacctgc tgattaaaaa tggaacggtg ctggatggaa ccggcgcgga
    39481 agcgattcat gccgacatcg gaatttgcgg cgagcggatc gtcgagatcg gcgacctctc
    39541 aaaagccgaa gcgcggcaaa ccatcgacgc caccggccaa atcgtctgcc ccggttttat
    39601 cgatgtccat tcccactcgg acgcctattt gctgatcgag ccgcgcgctt cgagtaaagt
    39661 atttcagggc gtcacgacgg aagtgtgcgg caactgcggc gcgtcggccg cgccgcgtct
    39721 cggcgacgcg aagctgccgt ccgactggca ggataagccg ttccccggaa cgtggcgcac
    39781 cgtcgccgaa taccgccgcc ttttcgaaca ggtaaagccc gccgtgaacg aggcgctgct
    39841 gatcggccac aacacgctgc accgcggcat cagcggctat gagccgcgcg gcgcgacgcc
    39901 cgacgaactg aaggaaatgt gccgcgcgct ggaacaggcg ctcgacgaag gcggatgcgg
    39961 cctgagcacc ggtctgattt atccgcccgc ctcggccgtg ccgtccgacg aaatcatcgc
    40021 gctggcaaag gtctgcgccg ggcgcaataa aatctacacc tcgcacatgc gcagcgaggg
    40081 ccggcaattg ctggaggcga ttgacgaagc gctgatgatc ggccggacgt ccggcgcgcg
    40141 ggtacagatt tcgcatctga aaacgtcggg caaggacaac tggcataagc tcgacgccgc
    40201 gctggcgaaa attgaagcgg cccgcgccga aggcattgaa gtcgccgccg accgctatcc
    40261 gtacaccgcc tcctgcaccg atctggatat tacgctgccg ggctgggcgc tctacgacgg
    40321 caaggccgcc gtgctggcgc ggctggcgga cgccgatacg cgcaaaaaaa tccgcgccga
    40381 aattctcgaa gggcgcggcg agttcggctt cggcatcatc atgatcggtt cgaccgagca
    40441 tcccgacctt cagcggttta aaggcctgta tctgcccgac gcggccaagg agctgaacat
    40501 ggagccggtg gatgcgctgc tccatattgt ggaaaaagat gaactgcgca ccggcgggat
    40561 tttcttcggc atgagcgagg agaatatgtg gaagattctg gcgcagccgt gggtgatgat
    40621 cggctgcgac gcgtcgatcc gcgcgccgga cggcattctg agcaaagacc atccgcatcc
    40681 gcgcgcctac ggcagtttca ccaaagtgct gcgcgccgcg ctggacggc
//
LOCUS       DAOPZO010000003        36410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_1050_length_36410_cov_10.932416, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000003 DAOPZO010000000
VERSION     DAOPZO010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 36410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36410
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1050_length_36410_cov_10.932416"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="PKI68_00370"
     CDS             complement(<1..1277)
                     /locus_tag="PKI68_00370"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNX52176.1"
                     /translation="MPELKINGRSVTVDSGATVLEAAQQIAVAIPTLCHLKGFMPETS
                     CMLCAVKDNTSGRMIPSCSARAVDGMDIDTECAEVQAARRDILNLLLSEHAGDCEAPC
                     KRICPAHLDIPLMLREIQRGNMESAAWIALRDLAIPAVLGHICPAPCEKGCRRGQLDT
                     SITIRALHREMGRACPPERTARRAVPAKKIAVIGSGAAGLSCAWNLRLLGYDCTVFDV
                     LPVAGGSLRNEPALPTEVLDAEIRRLQSAGIEFRLESAPPPDGFDAVVEPEEHRLAVK
                     AVANGKAAARTLNGTATVDRFDSKIGRLRDSELQELVKNSGAGFQPALEQAGMPAPLT
                     EAARCLHCDCRKPESCKLRKYAEQYGADQREFPADERVHLQLIGHNEAVVFEPGKCIK
                     CGLCVKITKRAGGNLGLTFTGRGFNTQVTVPFG"
     gene            complement(1402..3747)
                     /locus_tag="PKI68_00375"
     CDS             complement(1402..3747)
                     /locus_tag="PKI68_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNX52177.1"
                     /translation="MSEKINIPEVDCIVAALGREASAVIPILQAVQKEFKYLPEAALR
                     RICEISDITPAQIEGVASFFSQFRRTPVGRHMISVCDGTACHVKGAEAIHGSVLNALG
                     IKEGDTDAAGLFTVQKVACLGCCTLAPAVQIDGVTYGHLRTDTVSNMITDFLENESNR
                     TPRSFQKSEDGVSRGEVRIGLGSCCVAGGSAKIRDALEDSLAALDCKVDIKPVGCVGM
                     CHRTPLMEVVIPGEEPVLYAKVKPEDIPEIIERHFRSEKPFIKVRAAAESWLKKLYTD
                     EAHGVPERYAMDVREKPVADFLGRQQHIATEHGGMLNPSDMEEYQRLGGFEMLKTVLL
                     RNDPEGLISEIEKSGLRGRGGGGFPTARKWATVRKAPGAKKYIILNGDEGDPGAFMDR
                     MIMESYSYRVIEGMIIASFAVGATEGVLYIRAEYPLAVKRIRKALADCEAAGLLGGNI
                     LGSGHSLKLRVFEGAGAFVCGEESALIASLEGRRGMPNYRPPFPAEQGLHGCPTLVNN
                     AETYSVVPWIIRHGAESFARFGTEHSKGTKVFSLAGKIERGGLIEVPMGITINDVVNR
                     IGGGVADGRKFKAVQIGGPSGGCIPASLGDTPVDFEALKEVGAMMGSGGFVVLDDRDC
                     MVEMARYFLSFTQSESCGKCTPCRVGTKRMLEILTRLCEGKGRPGDIEKLEQLARTVQ
                     SQSLCGLGKTAPNPVLTTLRYFREEFEAHIAGRCPAGKCKKLITYSISDECIGCTKCA
                     VACPVEAISGEPYSVFKIDPAICVRCDTCRQVCPVDAVEVK"
     gene            complement(3845..4231)
                     /locus_tag="PKI68_00380"
     CDS             complement(3845..4231)
                     /locus_tag="PKI68_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNX52178.1"
                     /translation="MTETTEQQEELVSQFIDAAIEVHRELGPGYDESVYENALGVEMK
                     LRGIPFERQKSIEVFYKGIRVGEGRLDLFLGGEVVVELKAVDQLNEKHVAQVISYLKA
                     TKKPLGLLLNFHEFLLKKGLKRIIYS"
     gene            complement(4272..5990)
                     /locus_tag="PKI68_00385"
     CDS             complement(4272..5990)
                     /locus_tag="PKI68_00385"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNX52179.1"
                     /translation="MEEKSEFKKRPVPAIAAQSVAMAAALFSLILCVLLIADFVRIQK
                     MDPLNDPHLLQLREQLAVATDGGAALTEQIRTFDLYARRAFFSNQEQRRMGGLLLLGG
                     AAVCFIALKLSRLWNPELPKIGKPEPVDYRELNALFRQLMAVTGVLLAAVALFLAFAV
                     KSDLSVVLEQSKKQPAVPAAYVTQTAPPVQFMDEAKTNWPSLRGPGGTGVAYFTNAPT
                     GWDVKTGAGVLWKTEIPVPGFNSPVIWGRRIFMSGADDEGQEVFCFDADTGKQLWTKT
                     VKTAAKLPEVSKDTGYAAPTMTTDGRRVFAIFASGELAAFDFDGNPVWQKNLSVPENP
                     YGMGSSLITDGERLFVQYDHRNAQKVMAFDCATGSPVWQTERKHISWSSPALIKTGSG
                     PQLILNDEENVTAYDPVSGKQIWQVKCLGGEVAPSPAFNGKDIVFVANEYAQASTLKL
                     TGGTPKILWQYEKYLPEISSPVAAENFFFIATSAGDVVCLDAATGKEKWKQEFDEGFS
                     SSPVLAGDRIYAIDVAGVVHIFAAAGEYKEIGTIEMGEPVYATPAFMNGRIYIRGDDH
                     LYCIGK"
     gene            complement(6049..7347)
                     /locus_tag="PKI68_00390"
     CDS             complement(6049..7347)
                     /locus_tag="PKI68_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX52180.1"
                     /translation="MIPAFKFQVSDFAVRVSEFRFPMPEFASGYKHPAVYTPAPSSLM
                     AGWDIVALIAALTLAAIFVLKRRKRREIFLLTLFSLIYFGFWRNGCICAVGSVQNVAA
                     GFFNPAFGVPLTVLIFFAAPLIFALLFGRVFCAAVCPLGAIQEAVAIKPVKVPPAVEK
                     VLSIAPVIYLGLAMLSVAAGAGFLICRYDPFVGFFRMGASFSLLIFGGLMLVLGIFVA
                     RPYCRFLCPYGVLLNWASKLSRRHATITPAECIRCGLCADSCPYNAINEPSPEAAPES
                     RSRGARRLGLLILLLPVLVALGAGTGSLLREPLSRMHPTVRLAERIAGEELGRYTGNT
                     IESAAFRASDTPNEKLYAEAVALKERFGRGGKWFGAFIGLVIGCKLIGVSIRRKRTDY
                     EPDRGTCYSCGRCFKYCPVKASCREKPAGSTPDTAEFRQY"
     gene            complement(7344..7982)
                     /locus_tag="PKI68_00395"
     CDS             complement(7344..7982)
                     /locus_tag="PKI68_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX52181.1"
                     /translation="MKTTKRRDFIKNLARGAVLAGIAGVCGMLGLRKNKYQCSGRCGT
                     CKPANGGVCPLDSAAGSGFVWQLDPEKCTQCGRCATHCVLAPSAVKCFHSYSICGYCD
                     LCGGYLRPDAKERNTGAENQLCPTAAIRRKFIEDPYYQYTIDEELCIGCGKCVRGCST
                     FGNGSLYLQVKHNLCVNCNECAIARACPSDAFRRVPASEPYLLKSNQEKRIS"
     gene            complement(7979..8929)
                     /locus_tag="PKI68_00400"
     CDS             complement(7979..8929)
                     /locus_tag="PKI68_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52182.1"
                     /translation="MSSTTKSWIWFAVLLTLIAGAGIYVGTNRRAEPGKSFSYSVDDY
                     RQVDPALLRYAEADGLRPAIKKLSALAVAPDGKIYVAGENAIEIFPDRAVRPVDGTPV
                     CIAVDDDGTVFAGLRDHIEVVSKDGKSTAWPSPGEKACLTSVAVDDRYVYAADAGSRR
                     VWRYGKAGGAPFEIGAKDEANGVRGFYIPSPFFDLAIGTDGSLWVVNPGYHALENYSP
                     EGRLISSWQNSSFGIQGFSGCCNPANFALMPDGSFVTAEKGLPRVKIHNLDGSLRCVV
                     AAPDQFDNGVTGLDVAADRQGRVYVLDPARGAVRIFEEKK"
     gene            complement(8926..10362)
                     /locus_tag="PKI68_00405"
     CDS             complement(8926..10362)
                     /locus_tag="PKI68_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNX52183.1"
                     /translation="MKQKTTSLMATLPVLILCGGLAVYLLSRWFAYDPARGLTRSLPG
                     MDGSPAGTKQPVAGNLNVDLTGTLEKFDGVPSGLPGAWPRFRGPDSDNICKAEVKLAD
                     SWPESGPEILWSVALGEGHAAPAVFNGCVYLFDYDEAKRADALRCFSLADGKEIWRHS
                     YALPAKRNHGLSRTIPAVTEDCIVTIGPRCHVSCVETATGRFRWGIDLQKEYGTTEPL
                     WFTGQCPLIDEGRAILAPGGPDVLLMAVDCKTGAVIWKTPNPDGWKMSHSSVMPLTLH
                     GEAMYVYAAVGGLAGISKNGKLLWQIPWSASVIAPSPVPVPGNRIFITGGYGAGSLMV
                     QIDKTADGFTAKEVARTGPQEWLASEQQTPISHDGLLYGIMPKDGGALKQQFVCYDPD
                     GGLVWSSGKTSRFGLGPFLLADGKFFILGDDGTLTMLTASRDGYKQLAQHKVLNGQDA
                     WGPIAIAGTRMLLRDSTRMVCIEAGVKK"
     gene            complement(10399..11754)
                     /locus_tag="PKI68_00410"
     CDS             complement(10399..11754)
                     /locus_tag="PKI68_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNX52184.1"
                     /translation="MVHKAGICTVAGLVFFTTVFAADWPQFCGPDRNNTSTETGLAHS
                     WPAAGPAVLWSAKVTSGYAGPAVKNGSVYLLEHDGVRSSVRCLALDSGKELWTCSFDD
                     PGSVDKKYPGTRGTPTVTDDTLYAVTLLGTVVCVDLKSHQIKWQHNLIKEYGRKRSGW
                     GVAQSPVLAGNLVIIAPLTATASLVALDKDSGREVWKTPGFSGDGYVSPAMISIDGEE
                     QILMVAGGAKPAKPARRAKPADAAAAAAAAAENEEPKGTPLPTYVFAVSPKDGKVLWS
                     YEGWSCEAPIPHPVQVNRNTLFITSGYKSVSALIKVEKKEGGCEIKELFKTERAATQI
                     EQPVFVNNYLFVGGTTKNPRRGLVCMDLEGNVKWESGKAEGGPAFNDLNIIAADGMLI
                     GLDGDSGKLHLIEASPKEFKVLASAAVLPEKGQTWAPIALSGGKLLVRDHTVMKCLNL
                     K"
     gene            complement(11792..13414)
                     /locus_tag="PKI68_00415"
     CDS             complement(11792..13414)
                     /locus_tag="PKI68_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HNX52185.1"
                     /translation="MGAEKKHFEAALASSRKLLLAGLTPAGRWEGELSSSALATAVAS
                     FALKTAGKTGLAERGFQWLKTHRNADGGWGDTPESPSNFTTTLLCRCVLDEETGTPQE
                     VVRSILEHYGADRTFSVPILTMCMLAGKLGDDWSVVPQLPFELAVFPHRFYRFLRLPV
                     VSYALPALIAIGLVRHVSRPAHGSFVRQRIIPRALDILQKIQPESGGFLEAAPLTGFV
                     AMSLSAAGYGRHPAAQSCFRFLENTVRADGSWPIDVNLSTWVTSLAVRALDGDLPDKQ
                     KNSIRSYLLENQRQEEHPFTHAAPGGWGWTYEKGGVPDADDTAAALIALRRLGCDADA
                     PEKGIRWLLDLQNRDGGMPTFCKGWGKLPFDRSCPDLTAHMIRAFIVWRGAVDSGLSA
                     EIEHSIRRGLRYLKKQQRADGSWLPLWFGSQANRNHENPVYGTVRVLEALRELDEAEF
                     PVVGEMRRRGFLWLEAAQNPDGGWGSSVEETALAAGLTGKGLERLLEMTGNGTVFPAA
                     PIGLYFARLWYSEKMYPLIFTVEALQRYTPRS"
     gene            complement(13521..14144)
                     /locus_tag="PKI68_00420"
     CDS             complement(13521..14144)
                     /locus_tag="PKI68_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52186.1"
                     /translation="MENTVKFFRRNLPHWLVSDRSYFVTIRLHGTLPQTVMRTLQEKR
                     NSLEASSPDSPKLLHLQRRQFLKLEKILDVCESGRAWLADPDIASMVLKNLDWLRAER
                     GWRIYAGVVMPTHLHLLMRSEDGRSGELPNDLAHFKRFSAHKVNQILGRSGPFWAREQ
                     FDHWIRNREKFEGTIRYILNNPMKAGLVTDWRKWPWYCLDESVKELI"
     gene            complement(14288..15067)
                     /locus_tag="PKI68_00425"
     CDS             complement(14288..15067)
                     /locus_tag="PKI68_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HNX52187.1"
                     /translation="MQSLADRLICTAACSRRFLEQESRSEIARFILSRQNPGGGFRGK
                     SAESDLYYTVFAAAGLKALGWRIPVWPLWKYIRPFGLGEELDLVHLCCLIRLRTAFPM
                     TGKTRRQLFRNMEELPAESAYDFFLKQLTGDCLGIARLPAAPQPVSLADPTPNLAAAV
                     IVNRTSEKVAAGVLLNRVCRSGGFAATDQLNSADLLSTATALFALRILRADLDPVRRP
                     CMEFIESLWRDSGGFAGHVTDEFEDVEYTFYALLSLGCLME"
     gene            complement(15072..16595)
                     /locus_tag="PKI68_00430"
     CDS             complement(15072..16595)
                     /locus_tag="PKI68_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNX52188.1"
                     /translation="MKQTAAKYGVPADRFEREQVLLAVSQYAAEHQLVPPLSMEELRD
                     HAGNIAAHPGLIDYVMVLLNNEVWRDTVAAIPYERRLLLLPQCLRDVKECPAKPDEFG
                     LLCEECGRCNIGGLQALAEELGYVVLIAEGSTVVSRLLEGGKVDAVVGVSCLSALEKS
                     FSRMADGAIPGLAIPLTVDGCAGTEIDVEHVRDAIRLKSSTPWKNTLDTVRLREVVDG
                     WFSASGDWNTETRTGQIACEWLLKDGKRWRPYLLACTYSALNGGTTELPDEVRRLAIA
                     VECFHKASLIHDDIEDGDDLRYGEPALHRQYGLAVAINAGDLLVGEGYRWIASVETRT
                     AGLLRIAAENHRTLCIGQGEELYGLANGKTFTSKEIIEIFRRKTAPAFGAALLLGAVA
                     AGADRALLETLQSFSESLGIAYQIRDDLEEYKSGEARDLRASLLLALANDHSPSLRLT
                     AETAQLSHFFDELMIPEKAAQLLEHYKNEAVRALNPLKNALLKSLLRRVAAKMLGGG"
     gene            complement(16595..17788)
                     /locus_tag="PKI68_00435"
     CDS             complement(16595..17788)
                     /locus_tag="PKI68_00435"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HNX52189.1"
                     /translation="MSATLMKRMLTEPDKRLLWKFIYNFGFKGMRSIEKFQKRLKRGE
                     YFPAFLFLSVTNACNLACQGCWVSQTNPPRQIAPGTLDNVITECKAQGSCFFGILGGE
                     PLLYDGLFKVFEKHPDCYFLLFTNGTMITDAVAKEMRRIGNISPLISIEGLEEVSDVR
                     RGGNKVYEQTLAGLANCRANRLVTGVCTSVCKSNIDDLATGKFVNDAAALGAHYLWYY
                     IYRPVGPNPSPELALDAEQITRLRRFIVDIRLTAPLVVVDAYWDDQGRALCPAAVGIA
                     NHVSPDGYIEPCPPLQFARDNIGDGTGLYDLFNRSKFLADFRRMCCKTTQGCIIMEHP
                     GQLADFIRSTGAVDSSGRGTLLAELGRTCSCGSHHQPGEEIPERSRAYRFAKRHWFFG
                     FGAYG"
     gene            complement(17785..18480)
                     /locus_tag="PKI68_00440"
     CDS             complement(17785..18480)
                     /locus_tag="PKI68_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNX52190.1"
                     /translation="MSANKTLLICRCAGRRLLNREALEPLLRQDGAIPVSDLCGAAAA
                     KDPVLGQIAGADAPVVCACHPRAVRALFAFAGFPLPQNTEFINLRIPSGGPSAESDTA
                     WYPVLDEDRCTNCGQCFEFCLFGVYEKDSAGKIRVVNPHSCKNNCPACARICPAAAII
                     FPKVGEAPINGAEISDEEALKANIRINVDEILGGDVYAALNARKQKRRSLLDQKKVEQ
                     ALAERKKCSEDKA"
     gene            complement(18467..19654)
                     /locus_tag="PKI68_00445"
     CDS             complement(18467..19654)
                     /locus_tag="PKI68_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNX52191.1"
                     /translation="MGKWMLSLMVAAIAAGGYCNDWPVWRGPDRNGISKETGWNPKNA
                     KKLWTKELGDGYSSVSVKGGRLYTMGLKSGDEKTDTVYCLDAKTGNEIWSYSYSCQPG
                     PYKGPRATPVCDADSVYTLSRDGQLICFDAGSGKVKWKTDVLAGSGSQNIKWGLSSSP
                     VIEEDLILLNAGETGMAVDKNSGKIKWKSEGAASYASPVLFDRNGTRCAAIFSASGLQ
                     IVEALTGKKISSFDWETKYKINGADPLVIGDRIFISSGYDHGCAMLDFSSGALKPVWE
                     NSLIKAHFGSCVCIDGFIYGVDGQAKGKSVLRCVSAADGSEKWSAPVSFGSLMAADGR
                     LIVLDEDGSLSFVDVSAKKYNEISKLDTGIGPLCWTAPVLANGIIYCRNDKGTLVAID
                     VSK"
     gene            complement(19762..20283)
                     /locus_tag="PKI68_00450"
     CDS             complement(19762..20283)
                     /locus_tag="PKI68_00450"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HNX52192.1"
                     /translation="MNELKLIEPLILTLRNQKVILDADLAEIYGVSTKALNQAVKRNI
                     DRFPEDFMFRLTEDEKEEVVTNCDHLVQVKFSHQLPAAFTEHGAIMAATVLNSPEAVA
                     MSVFVVRAFVRMREQLAANAAILKRLAEIDKTLLEHDRALGVIWEQLQPLLAPPPEPS
                     NRQIGFDCHRKDE"
     gene            complement(20361..21113)
                     /locus_tag="PKI68_00455"
     CDS             complement(20361..21113)
                     /locus_tag="PKI68_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HNX52193.1"
                     /translation="MSAKTKADWTPELDKENDRAVWAHVIPFMAWLLMMSLLGDPSGT
                     RYAVQTAGGIILLLICRPWRWYTPPQLKNVPLALAVGLLVFVFWVGLESEFFKSAFPK
                     ISEFYERYLVGLTKFGKLREPLELGANGLYHYDPRATGWPLFWIHMLGTTLVIGAIEE
                     FMFRGFVYRWMQGSPFFRKDPGVMDSKMCILVAALFAVEHNEWLMGFLCGLFFTWLMV
                     KTRDIWAAIWAHAFTNGLLGWYAVHTGAYWFW"
     gene            complement(21168..21635)
                     /locus_tag="PKI68_00460"
     CDS             complement(21168..21635)
                     /locus_tag="PKI68_00460"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HNX52194.1"
                     /translation="MKMLKRAGQVVLFVGIAFLPALSAVFVQTGGWYEALYKPEWNPP
                     AAVFGPVWSALYLIIGLAGYFAWTRGPREHRRAEFSVYGAQLVLNALWTPVFFGLHRP
                     DLALAVLILLWFQILLCIALFSLRSFAAALLMLPYFIWISFAGALNATILFIN"
     gene            complement(21890..24274)
                     /locus_tag="PKI68_00465"
     CDS             complement(21890..24274)
                     /locus_tag="PKI68_00465"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNX52195.1"
                     /translation="MNAPIRLLLVTFFTAATRSLFAAEAPADQFKFLHVGEVQPRGWL
                     LEQIRMDMTNGYGPVMDKLTPKHILPTFFDSAKKTTPDKTWWPGETTGVWLDGLVRAA
                     YLSGDEPAKRRADELVTQILAMQEADGYLGVMPKAERFQSPIHVQNGDLWTQACLYRG
                     LLAYYELTGREDVLSAVQRATQLVMSKYGPDRPYWPGSIGRGGPPHSLMFVDVCEELY
                     RLTGDRSYVAFAKFMYDGYNAIPEVREFDSLLKNLSDPNKPLNGHGAHTVEHMRVPLF
                     LAYTTGEAKYRAAADNFLLKLSRHQNAGGACISDEDILERAGSPYIGSEYCTQGFLVF
                     TLQSGVEKTRDVKAADAAEVLAFNYAQGARLRDGKGNQYCSVDNQYAAVSKKNGGRMK
                     LSPTHEEVAACCSVGALCFFPYFTDGLWMKTADGRGLVAVNYAPNELRTSIKDVKIQI
                     TSDTGYPFEDEIRMTVTPEKPVACSIRLRIPAWAGSMNVVAAGATMVDDSGWRVLTKE
                     WKKGDHITVSFKPDVERKTMANGEVYWKRGPLVYALPIPSESKQIKTYPVAGFGDYEY
                     TPKAGAFWDYAAEEKGEAFNCVQSAVQGNPWITAPVRLTGRLINRKTGQSEPVELQPM
                     GVNLLRRVAFPQVKGAKALEAQAQALKSDLNLARSAKVAASTSASSSLPEALVDGVAQ
                     GLPDNPAAEWTSNQETTGAKVKLTWAKPVKIEDVWLFDRPNEKDQVLAAWINFSDGTS
                     AMVGELPNDGTAPFQLNFPEKIVKWMEVIITRVGPKTMNAGFSEIAVFQKAPTE"
     gene            24352..25467
                     /locus_tag="PKI68_00470"
     CDS             24352..25467
                     /locus_tag="PKI68_00470"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX52196.1"
                     /translation="MNILHADSDHSALTGGKARHRHRPVLHPPAMPILLKNQTADELH
                     RQLGITARQARMIQAVVIRKGQLPEVQAEISEKALKALRAATEIPNLTVSDKQVSPQD
                     GFAKYLFRGDGPEPFEAVRIPLLHREEDQKYIVCVSSQVGCAMGCSFCYTGKMGFIRN
                     LSAWEMVDQVLKIRDDSDHPVRGVVFMGMGEPLLNYDEVIRAARILSEPCGGAISAKA
                     ITVSTSGIAPAIRRFTAEKQPFRLVVSLTSADPQKRRDLMPVEGLHPTAELMDALRER
                     HAVTGERTTLAWTMISGLNTGEEDAKQLAELTRGLPVKLDLIDVNDPSGKYIPPSPEE
                     LHAFRDALRLHLAAPVARRYSGGKDIGAACGLLAGQK"
     gene            complement(25746..26294)
                     /locus_tag="PKI68_00475"
     CDS             complement(25746..26294)
                     /locus_tag="PKI68_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52197.1"
                     /translation="MKRILKIAKWLFLSWGVLSLVAAIGLAGVIAYSLSVGNRTKADQ
                     ASPHDVRFVLNWCGLGDSRTESVVHSYVSSRSFTGDHLDAYAIKISSVTVEELTASTD
                     EVRGRWYRGDQLPKVLDDAVSFIGGWLHEIPWFPSEDELRSGDIYIYSWSIYCHGVRP
                     SAAEIIFVRPKDKMVFFFGGKT"
     gene            26700..>27043
                     /locus_tag="PKI68_00480"
     CDS             26700..>27043
                     /locus_tag="PKI68_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52198.1"
                     /translation="MNPQSKIENHKSSIELMSPAGSEAALAAAIRAGADSVYFGVGKL
                     NMRARSAGLAPEDLPKIARRCRTAGVKSYLALNTIVYDGEMEEMHALCDAAKAAGVSA
                     VIATDIATIEYAR"
     assembly_gap    27044..27143
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <27144..27827
                     /locus_tag="PKI68_00485"
     CDS             <27144..27827
                     /locus_tag="PKI68_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNX52199.1"
                     /translation="YMSLAQYDHSANRGACFQPCRRSYRITDEETGAELVIDNKYVMS
                     PKDICTIQYLDKITAAGISVLKIEGRGRSADYVEVTTRVYREALDALAAGTYTPDKLD
                     PWVAELGKVFNRGFWHGGYYCGTKLGEWAGAADSQATEQRDEVGVLNNFYGKGNIAEF
                     TLRRHSIKTGDSLLIEGPTTGALRFTVEGLRVNGEPSESAQPNDAVTLALPRKARRQD
                     KVFLLTLRG"
     gene            27935..28342
                     /locus_tag="PKI68_00490"
     CDS             27935..28342
                     /locus_tag="PKI68_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52200.1"
                     /translation="MSRTIGLWIDHKEAVLVSIKDGALSVRRVESEVGTRFSPSGGWK
                     AGGTPVAQSIAKEQTADERRKHGLRAYYQELIKAVGGAEELFIFGPGEAKNELVKEIQ
                     KIKGSPVRIAAVETCDKMTEPQIAARVRKFFKV"
     gene            28429..29019
                     /locus_tag="PKI68_00495"
     CDS             28429..29019
                     /locus_tag="PKI68_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNX52201.1"
                     /translation="MRITSVPVVLLLALSFAFCGCGKKPQPASVPLPTAEAVAAYAEA
                     AFNGNARQVAAALQNGMPVDQRDKDGNSGLMLASFNGSVETMQLLLNAKAAVNLRDNK
                     GRTALMFAASGPFPEAVRLLLDKGADVNAVDAVDHFSALMFAAAEGLDPVVDILLKHG
                     ADPKMKDKDNDTAAKFARDRGFTGLAEKLQALIDAP"
     gene            29033..29845
                     /locus_tag="PKI68_00500"
     CDS             29033..29845
                     /locus_tag="PKI68_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNX52202.1"
                     /translation="MTEDQLSDAGSYKFGRFQKNAEELARLKMQATVAIDIEREVWKN
                     AGLKPGMNVLDLACGPGFISCELARMAGTVTGIDISEELIFTAHQAKVSEKADNVSFL
                     TGNVYDLALPENAFDFVYARFLFQHLEKPQLALSNILRVLKPGGILCVLDIDDNWTSF
                     APESAAFVKFIRKAGAGQKRKGGNRLIGSQLFGLLNGAGFKEVSVKIQPITTEDLGVK
                     MFLGVAVLFRLEMLNKLQKLLALPQLKKIKSAAQAPNAWGSVGVFIATGTKA"
     gene            29850..30653
                     /locus_tag="PKI68_00505"
     CDS             29850..30653
                     /locus_tag="PKI68_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNX52203.1"
                     /translation="MRFKGNSDQSSARYWDDLAELYQRETRISTDDFHYGPLLPGDST
                     LRLLPVAGGLRALEIGCGAGQNSIFLAKQGAQCVAIDISEAQLAHGRKLAAAENVSVD
                     FRRISMDAMGDLGKFDLIHSSYALPFSADPQKVIAAVAARLKPGGTFLLTTGHPLYAG
                     EWLDIGDGEDGLFLPDYFRPEPDVRMSLDDKTMSAAHYWPISTLTEWIHSAGLTIVRL
                     LEPAPMPIPEMSEAEIRANVPYDSPGWRELYSQLARIPVVLVLKCSLIN"
     gene            30747..31001
                     /locus_tag="PKI68_00510"
     CDS             30747..31001
                     /locus_tag="PKI68_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52204.1"
                     /translation="MKKVLLFSMIAIAGLTVSAVDMTKEVYIAKIKAVAERKGRAFDA
                     EKVAADFDKLDKNKDGVWSDEEQGKVPAKPKDPAATPAAE"
     gene            31490..31711
                     /locus_tag="PKI68_00515"
     CDS             31490..31711
                     /locus_tag="PKI68_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52205.1"
                     /translation="MKKVLLFSMIAIVGLTVSAAETTKADYIAKAKAAAEKKGRAFDE
                     AKTAKAFDKLDTNKDGVLSAEEQAAPKAK"
     gene            31857..34274
                     /locus_tag="PKI68_00520"
     CDS             31857..34274
                     /locus_tag="PKI68_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNX52206.1"
                     /translation="MAKQSKQSEIVRSPKREAVGVILLALCLLSFLCVFSFNPEDIGW
                     LHNPPASPPHNLVGPFGAWFAFIGFLLFGVGGYLLPFLLLTGGLMMTFRSYERGWPVW
                     AWLVAGLSSLSSLFEYQPGLWAGLKDILNLGSPGGLIGQLLAIRTVGKLINHTGAGIV
                     FVTVFILAVLQLTGMHPFTPFTFLWGKIQEFRAARKEARAAAPKTKFSRQVPPAEKEK
                     PKKAVLSTPEPAPAEGELFVPPVSIPKAEAKAETKPEPVKPQPKVKPVAPPPADELSN
                     VSVNIAPGRDESGYQLPPLTILDELPSLASRTSTENTEEKAKILQATLEEFDVQGQVT
                     HVETGPTVTSYEITPAPGVRVERISALERNIALALRAQSIRIQAPIPGKGAVGIEVPN
                     SKSTAVYLRELAESPAFHDPGIALPLALGKDVSGRMVVGDLSAMPHMLIAGATGAGKT
                     VCMNSLLTGLLLSRTPEQLRLILVDPKTFEFQAYENLKHLVVPIITDAKKVTLGLRWA
                     IKEMEKRYALFKKVKVRDIKGFNTRPIEKQTDLFGAAAEEDDQPKDVVPAKLPYIVIV
                     VDELAELMSVAQQEIEPCIVRLAQLSRAAGIHMILATQRPSVNVITGTIKANIPGRIA
                     FQVAQGNDSRTILDTIGAEKLLGKGDMLYLPPGKNKPVRLQGTWTSDGEMHRVTDFIR
                     TQGMPEFITEIKQKIENNRPELPDMDESDDLLESAVEVLRSTKRASTSSLQRRLKIGY
                     NRAARLMDILEEKGIIGPPTETGPRDILIDLDGEIPEHGGSSEPLSEAEAPQEFLDDE
                     EEHKPED"
     gene            34280..34873
                     /locus_tag="PKI68_00525"
     CDS             34280..34873
                     /locus_tag="PKI68_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX52207.1"
                     /translation="MATLGQQLKAAREAKGISESEAGAATKILTRIIVAMEADDFSGM
                     AAPTYAKGFIRLYAGYLGLNPDPLIEEYTARHTAAAKPLVDEASRLEQNRRPPPSFAA
                     IRKRIPVKFNPPAWLRIPNFTKGLKPYVVKDLRALAIGAAALLVLIVLAVSVSNCVRR
                     HGAEKAVKQPAEASARRLLDKPLPDLYLVEPGKIESK"
     gene            34959..35522
                     /gene="pgsA"
                     /locus_tag="PKI68_00530"
     CDS             34959..35522
                     /gene="pgsA"
                     /locus_tag="PKI68_00530"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNX52208.1"
                     /translation="MNPPNALTVSRFALAAVFMVCLSVSFPGAPLAAVIVFGVAALTD
                     ALDGHLARNVYGCSSFGKLMDPLADKVLTAAAFIGFVELRIMPAWMVTLILSREMMVT
                     GLRLLAIDKGLILSAGSWGKHKTIWQMVFISAVLLLRMVYWIYPWSFLESELYRQILL
                     WAGGIVTVLTVWSGIIYFRQNLSLLRN"
     gene            35561..>36410
                     /locus_tag="PKI68_00535"
     CDS             35561..>36410
                     /locus_tag="PKI68_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52209.1"
                     /translation="MANVREESWQIKRRSDLCAGTNEPFSDKEEIVTRLLFKDGEYVR
                     EDYKLSYWNGHQPDHGLSSWKSVFRVPPPPTEVVKKESAETLLRKLVAKEDADDINSI
                     YILAVMLERKRILAEKDVQIRDDQTKVRVYEHKKSGETFLVIDPELKLAEIEKVQEEV
                     VGLLGGKPPKTETVYHFEAILHALIEKHEKLLFPKRPVGGEAGIAQKLAASFFALLAG
                     KKNPYWEESNKVFNRFEDSETWGPAVTAYRDLAERIPQEVRFFGMEKPELAKQLEALA
                     IKEPKEL"
BASE COUNT         7729 a        11079 c        10086 g         7416 t
ORIGIN      
        1 tcgccgaacg gaaccgttac ctgcgtattg aacccgcggc cggtaaaagt cagtccgaga
       61 tttccgccgg cgcgtttagt gatcttcacg cacagtccgc acttgataca tttgcccggc
      121 tcgaacacga ccgcctcatt gtgtccgatc agctgcaaat gtacgcgttc gtccgccgga
      181 aattcccgct gatccgcacc gtactgctcc gcatatttcc gcagcttgca gctctccggc
      241 ttgcggcagt cgcaatgcag acatcgcgcc gcctcggtta gtggagcagg cattcctgcc
      301 tgctcaagtg caggctgaaa gcctgcacca ctattcttca ccagctcctg cagttcgctg
      361 tcgcgcagtc tgccgatttt cgaatcgaac cggtctacgg ttgccgttcc gttcaaggtg
      421 cgcgctgctg ctttgccgtt ggcgaccgct ttaacggcca gcctgtgttc ttccggctca
      481 accactgcgt cgaaaccatc cggcggcgga gcggactcca gccggaattc aattccggcg
      541 gactggagcc ggcggatttc cgcatcgaga acctccgtcg gcagcgccgg ttcattgcgc
      601 agcgaaccgc cggcaaccgg cagtacgtca aaaaccgtgc agtcatatcc cagcagccgc
      661 aggttccagg cgcaggaaag tcctgccgcg ccggaaccga ttaccgcgat ttttttggca
      721 gggacggctc gccgagccgt ccgctccggc ggacacgccc tacccatttc gcggtgtaac
      781 gcgcggatcg tgatcgaagt gtccagctgt ccgcggcggc agcctttttc gcacggcgcg
      841 ggacagatat gcccgagcac cgccggaatc gcgagatcgc gcagggcaat ccacgcggct
      901 gattccatat taccgcgctg aatttcgcgc agcatcagcg gaatatccag atgcgccgga
      961 cagatgcgtt tgcacggcgc ttcacagtcg ccggcatgtt cactcaaaag cagattcagg
     1021 atatcgcgcc gcgccgcctg aacttcagcg cactccgtgt cgatatccat tccgtcgact
     1081 gcgcgcgccg agcaggacgg aatcatccgg ccgctggtat tgtctttcac cgcgcaaagc
     1141 atgcacgacg tttcgggcat gaaacccttc aggtggcaca gcgtcggaat cgcgacagca
     1201 atctgctgcg cggcttccag cactgtcgcg ccggaatcca ccgttacaga ccgtccgttt
     1261 attttcagtt caggcataac caaatctacc tcatcttttc ccgcccataa gccggatcta
     1321 cggacggata cgtcctaagt tccggcttta ctctcccagt actgctggaa ctcagccgca
     1381 tccggcgtgg atccggcata gctatttcac ttccaccgca tccaccggac aaacctgccg
     1441 gcaggtatcg caccgcacac agattgcagg atcaatttta aataccgagt aaggctcgcc
     1501 gctgatcgct tcgaccgggc aggcaaccgc gcacttcgtg cagccgatgc actcgtcact
     1561 gattgaatag gtgatcagct tcttgcattt gccggccgga cagcgtccgg cgatgtgcgc
     1621 ttcgaactct tcgcggaaat accggagcgt cgtcagcacc gggttgggcg cggtcttgcc
     1681 gagtccgcac aggctctgcg attgaaccgt tcgggcgagc tgctccagtt tttcgatatc
     1741 gcccggtctg ccttttccct cgcaaagccg cgtcaggatt tcgagcatcc gtttggtgcc
     1801 gacgcggcag ggcgtgcatt tgccgcacga ctcgctttgc gtgaacgaca gaaaatagcg
     1861 cgccatctcg accatgcagt cgcggtcatc gagcactaca aaaccgccgg agcccatcat
     1921 cgcgccgact tctttcaagg cttcaaagtc aaccggagta tcgccgagcg aagcgggaat
     1981 acagccgccg gacggtccgc cgatctgcac cgctttgaac ttccggccgt cagcgactcc
     2041 gccgccgatc cggttgacca cgtcgttgat ggtgatgccc atcggcactt cgatcagtcc
     2101 gccgcgctcg atttttcccg ccagtgaaaa taccttggta cctttgctgt gttccgtccc
     2161 gaaccgggcg aacgactccg ctccgtggcg gataatccac ggcacgacgg aataggtttc
     2221 cgcattattc accagtgtcg ggcagccatg cagcccctgc tccgccggaa acggcggacg
     2281 gtagttcggc atgccgcgcc gcccttcgag cgaagcgatc agcgccgact cttcgccgca
     2341 gacaaacgcg cccgctcctt caaaaacccg gagcttcaga gaatgcccgc tgcccagaat
     2401 attcccgccc agcagtccgg ccgcttcgca gtcggccagc gccttgcgga tccgcttcac
     2461 cgccagcgga tattcggcac gaatgtagag cacgccttcc gtcgcgccga ccgcgaatga
     2521 tgcaatgatc attccttcga tcacgcggta tgaatacgac tccataatca tccggtccat
     2581 gaacgcgccc ggatcgcctt cgtcgccgtt gagaatgatg tatttcttcg cgccgggcgc
     2641 tttgcgcacg gttgcccatt tgcgggcggt cggaaatccg ccgccgccgc ggccgcgcag
     2701 tccgctcttt tcaatttctg aaatcaatcc ttccggatca ttccggagca gaacagtttt
     2761 cagcatttcg aaaccgccga gccgctggta ttcctccata tccgacggat tcagcatgcc
     2821 gccatgttcg gtggcaatgt gctgctgccg gccgaggaaa tcggccacgg gtttttcacg
     2881 cacatccatt gcatagcgtt ccggcacgcc gtgcgcttcg tcggtataaa gtttcttcag
     2941 ccagctctcc gccgccgcgc ggactttgat gaacggtttt tcggaacgga aatgccgttc
     3001 gataatttca ggaatgtctt ccggttttac cttggcgtac agcaccggtt cttcgcccgg
     3061 aatcaccacc tccatcagcg gagtgcggtg gcacataccg acgcagccga ccggcttgat
     3121 atcgaccttg cagtccagcg cggccagcga atcctccagc gcgtcgcgga ttttcgcgct
     3181 gccgccggct acacagcacg agcccagacc gatccgcacc tcaccgcgcg acacaccgtc
     3241 ttccgatttc tgaaaactgc gcggcgtccg gttggattca ttctccagaa aatcggtaat
     3301 catgttcgaa accgtgtcgg tccgcagatg accgtaggtt acgccgtcga tctgtaccgc
     3361 cggagccagc gtacagcagc cgaggcaggc cactttctga acggtgaaaa gaccggcggc
     3421 atcggtgtcg ccttctttga tgcccagcgc gttcagcacg gaaccatgaa tcgcttcggc
     3481 gcctttaacg tgacaggccg tgccgtcgca aacactgatc atatgtctgc cgaccggcgt
     3541 ccggcggaac tgcgaaaaaa acgaagcgac tccttcgatc tgcgccggtg tgatatcgct
     3601 gatttcgcaa atccgccgca gcgccgcttc cgggagatat ttaaactcct tctgaaccgc
     3661 ctgaagaatc ggaatgaccg ccgatgcctc gcggccgagc gccgcgacga tgcaatcaac
     3721 ctccgggatg tttatttttt cactcattac tacttctggt tttgaccgct aagtcgcgaa
     3781 gacgctaaga aagaaatctc cacgcattcc ctttgcttct ttgtgccttt gcggtcagcc
     3841 ctctttatga atagataata cgcttaagcc cttttttaag gagaaattca tgaaagttga
     3901 gcagcagccc caatggtttc ttcgttgctt tcaaatacga aataacctgc gcgacatgtt
     3961 tctcattcaa ctgatcgacc gcctttaatt caacaaccac ctctccgcct aaaaataaat
     4021 ccagcctgcc ctctccaaca cgtattcctt tataaaaaac ctctatgctc ttctgccgct
     4081 cgaacggaat gccccgcagt ttcatttcaa cccccagcgc attttcatag acagactcat
     4141 cgtaccccgg accgagctca cgatgaacct cgattgccgc atcgataaat tggcttacca
     4201 attcctcttg ctgttccgtt gtctcggtca ttcctctttc cttcgtgtct ttgcgtcttt
     4261 gcggtcacaa ctcatttgcc aatgcaatac aaatggtcat cgccgcgaat ataaattcgt
     4321 ccgttcatga aagcgggtgt ggcgtaaacc ggctcgccca tttcgatggt tccgatttcc
     4381 ttgtattcgc cggcggcggc gaaaatgtgc acgacaccgg caacgtcaat ggcgtaaatc
     4441 cggtcgccgg cgagcaccgg cgacgaactg aatccttcgt cgaactcctg cttccatttt
     4501 tctttgcctg tcgcggcatc gagacagacc acatcgcccg ccgaggtggc gatgaagaaa
     4561 aagttttccg ccgcgaccgg actgctgatc tccggcagat atttttcata ctgccacagg
     4621 attttcggtg tgccgccggt cagcttcagc gtgctggcct gcgcatattc gttcgccacg
     4681 aaaacaatgt ccttaccgtt gaaggcgggc gacggcgcaa cttcgccgcc gagacattta
     4741 acctgccaga tctgttttcc ggaaaccgga tcataggccg tcacattttc ttcgtcgttg
     4801 agaatcagct gcgggccgga gccggttttg atcagcgccg ggctcgacca tgaaatatgt
     4861 ttgcgctccg tctgccagac cggactgccg gtcgcgcagt cgaaagccat caccttctgc
     4921 gcgttcctgt gatcgtactg cacaaacagc cgttcgccgt cggtgatgag cgacgagccc
     4981 atgccgtacg gattttccgg aacgctcagg tttttctgcc agaccggatt gccgtcgaaa
     5041 tcgaacgcgg ccagttcacc ggatgcaaag atggcgaaca cgcgccggcc gtcggtcgtc
     5101 atcgtcggcg cggcatagcc cgtgtctttc gacacttcag gaagtttggc cgcggtcttc
     5161 accgtcttcg tccagagctg tttgcctgta tccgcatcga agcagaaaac ctcctgtcct
     5221 tcatcgtcgg cacccgacat gaaaatccgg cggccccaga tgaccggcga gttgaatccg
     5281 ggaacaggaa tttctgtttt ccacagtacg cctgcgccgg ttttaacatc ccagccggtc
     5341 ggcgcgttcg tgaaatacgc gacgccggtt ccgcccggtc cgcgaagcga cggccagttg
     5401 gttttcgcct cgtccatgaa ttgaacaggc ggagctgttt gtgtcacgta cgcggcaggc
     5461 acggcgggct gtttcttcga ctgctcaagt acaacggaca aatcactctt cacggcgaag
     5521 gccagaaaca gcgcgacggc agccagcagg acgccggtca cagccatcag ctgccggaac
     5581 agcgcgttga gctcccgata atccaccggt tccggtttgc cgatcttcgg aagttccgga
     5641 ttccaaagtc tggaaagctt cagcgcgata aaacagaccg ccgcgccgcc gagcagaagc
     5701 aggccgccca tgcgccgctg ttcctgatta ctgaaaaaag cgcggcgggc atacagatcg
     5761 aacgtgcgga tctgttccgt cagcgccgcg ccgccgtctg tcgcaacggc cagctgttcg
     5821 cgcagctgca gcagatgcgg atcgttcaac ggatccattt tctgaatccg cacaaagtcg
     5881 gcaatcagca gaacacagag gatcagcgaa aacagcgccg ccgccatcgc gacgctctgc
     5941 gccgctattg ccggaaccgg gcgcttttta aattctgatt tctcttccat acaccaccct
     6001 ttatgagccg gatctacggg cgaatacacc ctaagttccg gctttacttc agtactgccg
     6061 gaactcagcc gtatccggcg tggatccggc aggtttttcg cggcacgagg ccttcacggg
     6121 acaatattta aaacagcgtc cgcagctgta gcaggtgccg cggtcgggtt catagtccgt
     6181 gcgtttccgc cggatggaaa cgccgatcag cttgcagccg atcaccaggc cgataaacgc
     6241 gccgaaccat tttccgccac ggccgaatct ctctttaagc gccacggctt ccgcataaag
     6301 tttttcgttg ggcgtgtcgg atgcgcggaa ggccgccgat tcgatcgtgt tgccggtgta
     6361 ccgccccagc tcttcgccgg cgatgcgctc ggccagccgg acggttggat gcatccgcga
     6421 aagcggttcg cgcagcaggc tgccggttcc ggcaccaagt gcaaccagca ccggaagaag
     6481 cagaatcaaa agtccgagac gccgagctcc cctgctgcgg ctttccggcg ccgcttcggg
     6541 agacggttca ttaatggcgt tgtaagggca cgagtctgcg caaagtccgc accggataca
     6601 ttcggccggc gtaatcgttg cgtgacggcg cgaaagtttc gacgcccagt tgagcagtac
     6661 accatagggg cagagaaaac ggcagtacgg ccgcgctaca aaaataccga gcaccagcat
     6721 gaggccgccg aaaatcagca ggctgaaact cgcgcccatg cggaagaagc cgacaaacgg
     6781 atcgtaccgg cagatgagga agcccgcgcc cgccgccacg gaaagcatcg ccagtccgag
     6841 atagatcacc ggagcaatcg aaagaacttt ttcgaccgcc ggcggaactt tgaccggttt
     6901 aatcgcgacc gcttcctgaa tcgcgcccag cggacagacc gccgcgcaga aaacacgtcc
     6961 gaacagcaaa gcgaagatca gcggcgcggc aaaaaagatc agtaccgtca gcggcacgcc
     7021 aaaggcagga ttgaaaaacc ccgccgcaac attctgcacg gagccgacgg cacagatgca
     7081 gccgttacgc cagaagccga aatagatcag cgagaaaagc gtgagcagaa aaatttcgcg
     7141 gcgcttgcgc cgttttaaca caaagatcgc cgccagcgtc agcgcggcga tcagcgcgac
     7201 gatgtcccag cccgccatca gactggacgg cgcaggcgta taaaccgccg gatgtttgta
     7261 gcccgaagcg aactccggca tcggaaagcg gaattccgaa acccgaaccg cgaaatctga
     7321 aacctgaaat ttgaaagcgg gaatcatgag attcttttct cctgattgga tttcagtaaa
     7381 tacggctcgg aagccggcac gcggcggaag gcgtcggacg gacaggcgcg ggcgatggcg
     7441 cattcgttgc agttgacgca caggttatgt ttcacctgca gatagagcga gccgttgccg
     7501 aacgtgctgc accctcttac acatttgccg cagccgatgc agagctcttc gtcgatggtg
     7561 tactggtagt aaggatcctc aatgaatttg cgccggatgg ccgccgtcgg acagagctga
     7621 ttttccgcgc cggtattgcg ttccttggcg tcgggacgca gatagccgcc gcacagatcg
     7681 cagtagccgc agatggaata ggagtgaaaa catttcacgg ccgacggcgc cagtacgcaa
     7741 tgcgtggcgc agcgtccgca ctgggtgcat ttctccggat cgagctgcca gacgaatccg
     7801 gacccggcgg cggagtccag cgggcagacc ccgccgttcg cgggcttgca ggttccgcag
     7861 cgcccgctgc attgatattt atttttccgc aggcccagca tgccgcagac tccggcgata
     7921 ccggcgagca cggctccccg tgcgaggttt ttaataaaat cccggcgttt ggtcgttttc
     7981 atttcttctc ctcgaaaatt ctgaccgcgc cgcgggccgg atcgagtaca tagacgcggc
     8041 cctgccggtc cgccgcaacg tcgagtccgg tcacgccgtt gtcaaactga tccggcgccg
     8101 caacgacgca gcgcagcgaa ccgtccagat tgtgaatctt gacgcgcggc agaccttttt
     8161 cagcggtgac gaacgaaccg tccggcatca gcgcaaagtt cgccggatta cagcatccgc
     8221 tgaacccctg aatgccgaac gaactgttct gccacgacga gataagccgt ccctccggac
     8281 tgtaattttc cagcgcatga tagccgggat taacaaccca cagcgagccg tcggttccaa
     8341 tggcgagatc gaagaacgga ctgggaatat agaagccgcg cacgccgttc gcttcatctt
     8401 tcgcgccgat ttcaaacggc gcaccgccgg ctttgccgta ccgccagacg cggcggctgc
     8461 cggcgtcggc ggcataaaca taccggtcat cgaccgcgac ggaggtcagg caggcctttt
     8521 cgccgggcga cggccacgcc gtgctttttc cgtcttttga aaccacttcg atatgatcgc
     8581 gcagaccggc aaataccgta ccgtcgtcgt ccactgcaat acagaccggc gttccgtcaa
     8641 ccggacggac cgctctgtcc ggaaaaattt cgatagcgtt ttcgccggcg acataaattt
     8701 tcccgtccgg cgcgacggcc agcgcggaaa gttttttaat cgccggacgg agaccgtcgg
     8761 cctcggcgta gcgcaggagt gcagggtcaa cctgccggta gtcgtcgacg gaataggaaa
     8821 aacttttgcc gggctctgcg cggcggttcg ttccgacata aattccggcg ccggcaatca
     8881 gcgtcaaaag cacggcaaac cagatccagc ttttcgttgt actgctcatt ttttaactcc
     8941 ggcttcaatg cagaccatcc gggtcgaatc gcgcaggagc atacgggttc cggcgattgc
     9001 gatcggcccc caggcatcct gtccgttcag caccttatgc tgcgcaagct gtttatatcc
     9061 gtcccggctg gcggtcagca tggttaatgt gccgtcatcg cccagtataa aaaactttcc
     9121 gtcggccagc aggaacggac cgagtccgaa gcggctcgtc ttgccgctcg accagaccag
     9181 tccgccgtcc gggtcgtagc agacgaactg ctgtttcagc gcgccgccgt ctttcggcat
     9241 gattccgtaa agcaggccgt cgtgcgaaat cggcgtctgc tgttcgctcg ccagccactc
     9301 ctgcgggccg gttcgcgcaa cttcttttgc ggtaaagccg tcagcggttt tgtcgatctg
     9361 caccatcagg ctgccggcgc cgtaaccgcc cgtgatgaaa atccgatttc ccggaaccgg
     9421 aacgggtgac ggtgcgatca ccgacgcgct ccagggaatc tgccacagga gtttcccatt
     9481 tttggaaatg ccggccaggc cgccgaccgc ggcgtacaca tacatggctt cgccgtggag
     9541 cgtcagcggc atgacggatg agtgcgacat tttccagccg tccgggttcg gcgttttcca
     9601 aatgaccgcg ccggttttac agtcgaccgc catcagcagt acgtcgggcc cgccgggcgc
     9661 aagaatcgcg cggccttcgt cgatcagcgg acactgcccg gtaaaccaca gcggttccgt
     9721 ggtgccgtac tctttttgca gatcaatccc ccagcggaat ctgccggtcg ccgtttcgac
     9781 gcacgaaacg tggcagcgcg ggccgatcgt cacgatgcag tcttcggtga ccgccggaat
     9841 ggtccgcgaa agtccgtggt tccgtttggc aggcagcgcg taggagtgcc gccagatttc
     9901 tttgccgtcg gccagcgaaa aacagcgcag cgcatccgcg cgttttgctt cgtcgtagtc
     9961 gaacagatag acgcagccgt tgaaaacggc gggcgcggcg tggccttcgc ccagcgcaac
    10021 cgaccagaga atttccgggc cggactccgg ccagctgtcc gccagtttca cctcggcttt
    10081 acagatgtta tcagaatccg gaccgcggaa acgcggccac gcaccgggca gaccggacgg
    10141 aacgccgtcg aatttttcaa gcgttccggt cagatccacg ttcaggttgc cggcgacggg
    10201 ttgtttcgtt ccggcaggac tgccgtccat gccgggcaga ctgcgcgtca gtccgcgcgc
    10261 cggatcatac gcgaaccagc gtgaaagcaa atagacagca agcccgccgc atagaatgag
    10321 gacgggcagc gtcgccatca gactggttgt cttttgtttc atgcttcggg cgcattcagg
    10381 aaacacatcc ggtctgtttc atttcagatt cagacacttc atcacggtgt gatcccggac
    10441 aagcagtttg ccgccggaca gagcaatcgg cgcccaggtc tgtccttttt cgggcagaac
    10501 agcggccgac gcaagtacct tgaactcttt cggcgaagct tcaatcagat gcagcttccc
    10561 ggaatcgccg tcgagaccga tcagcattcc gtcggcggca ataatattca gatcattgaa
    10621 cgcaggaccg ccttcggcct tgccgctctc ccactttaca tttccttcca gatccatgca
    10681 gacgagtccc cgccgggggt ttttcgttgt gccgccgaca aacagataat tgtttacaaa
    10741 aaccggctgc tcaatctgtg tcgccgcgcg ttcggtttta aacagttctt tgatttcgca
    10801 gccgccctct tttttctcca ccttaatcag cgcggaaacg gatttgtagc cggatgtgat
    10861 gaacagcgta ttgcggttaa cctggaccgg atggggaatc ggagcctcgc aggaccagcc
    10921 ttcataactc caaagcacct ttccgtcttt cggcgaaacg gcaaaaacat atgtggggag
    10981 cggcgttccc tttggctcct cgttctccgc ggccgcagcc gcagccgcag ccgcatccgc
    11041 cggcttggcg cggcgggccg gtttcgcagg cttggccccg cccgctacca taagaatctg
    11101 ctcttcaccg tcgatggaaa tcatggctgg cgaaacgtac ccgtctccgg agaatccggg
    11161 cgttttccag acttccctgc cggagtcttt atccaaagcc accagacttg cggttgccgt
    11221 cagcggcgcg atgatgacca gatttccggc cagtaccggc gactgagcaa ctccccagcc
    11281 gctccgtttt ctgccgtatt ctttaatcag gttgtgctgc cactttatct ggtgagattt
    11341 taaatccacg cagaccactg ttccgagaag cgtcacggca tacagggtgt cgtccgttac
    11401 cgtcggcgta ccgcgggttc ccggatactt tttatccacc gatccgggat cgtcaaacga
    11461 acaggtccaa agctccttgc cggaatccag cgcaagacac cgaacactgc tgcggactcc
    11521 gtcgtgctcc agcagataca ccgaaccgtt tttgacagca ggaccggcat agccgctggt
    11581 caccttcgca ctccacagca cggcaggacc ggcggcaggc cagctgtgcg ccagtccggt
    11641 ttccgtcgac gtattgttcc ggtccggtcc gcaaaactgc ggccagtccg cggcgaacac
    11701 cgtggtaaaa aaaacaagcc ctgccactgt acaaatccct gctttatgca ccatctgccg
    11761 actccttgtt taaatcaacc cgtgcgccat tctatgaacg cggagtgtac ctttgaagcg
    11821 cctccaccgt aaagatcagc ggatacattt tctccgaata ccacagacgc gcaaagtaca
    11881 gcccgatcgg cgccgccgga aataccgttc cgtttcccgt catttccagc agccgctcca
    11941 gtcctttccc tgtcagaccc gccgccagcg cggtttcctc aaccgaactt ccccagccgc
    12001 cgtccggatt ctgcgcggct tccagccaga gaaatccgcg ccgcctcatt tctccgacaa
    12061 ccggaaattc ggcctcatcc agttcgcgca gcgcttccag cacacgaacc gttccgtaaa
    12121 ccggattttc atgattccgg ttcgcctgac tgccgaacca gagcggaagc cacgaaccgt
    12181 cggcgcgctg ctgctttttc aaataacgca gaccccggcg gatcgagtgt tctatttctg
    12241 cagaaagtcc ggaatcaacg gctccgcgcc aaacgataaa cgcgcggatc atgtgcgccg
    12301 tcagatccgg acagctgcgg tcgaacggca gttttcccca gcctttacag aacgtcggca
    12361 ttccgccgtc acggttctgc aaatccaaca gccaccgaat ccctttttcg ggcgcgtccg
    12421 catcgcagcc cagccggcgg agtgcgatca gcgcggcggc cgtatcatcc gcgtcgggca
    12481 ctccgccttt ttcataggtc cagccccagc cgccgggcgc ggcatgagtg aacggatgtt
    12541 cctcttgccg ctgattctcc agcagatacg accggatgct gtttttctgt ttgtccggaa
    12601 gatcgccgtc cagcgcgcgg accgccagcg aggttaccca ggttgaaaga ttaacgtcga
    12661 tcggccagct gccgtctgcg cgcaccgtgt tttccagaaa ccggaaacac gactgcgccg
    12721 ccggatgacg gccgtatccc gcagccgaca aactcatcgc cacgaacccg gtcagcggcg
    12781 cggcttcgag aaagccgccg ctttcgggct gaattttctg cagaatgtcc agcgcgcgcg
    12841 gaatgatccg ctgccggaca aacgaaccgt gcgccggacg gctgacatgc cgtaccagcc
    12901 cgatcgcaat cagcgcaggc agcgcatagc tgaccaccgg aagccggaga aatctgtaga
    12961 agcggtgcgg aaataccgcc agctcgaacg gaagctgcgg cacaaccgac cagtcgtcac
    13021 ccagctttcc cgcaagcatg cacatcgtca gaatcggaac ggaaaaggtg cggtcggcac
    13081 cgtagtgttc gagaatagac cggacaactt cctgcggcgt accggtctct tcatccagca
    13141 cacagcggca aagcagcgtc gtcgtaaaat tgctcggact ctccggagtg tcgccccagc
    13201 cgccgtcggc attgcgatgg gttttcagcc actgaaatcc gcgttccgcc agtccggttt
    13261 tcccggcagt cttcagcgca aacgaagcca ccgccgtcgc cagcgcggaa ctcgacagct
    13321 ctccttccca gcggccggcc ggcgtcagcc cggcaagcag caacttccgc gatgaggcca
    13381 acgccgcttc aaaatgtttt ttctccgcgc ccatttctta tctttcacaa gccggatcta
    13441 cgggcttcgc cctaagttcc ggctttaccc ggtactgccg gaactcagcc gtacccggcg
    13501 tggatccggc aatctccggc tcagattaat tccttaaccg attcgtccag acaataccac
    13561 ggccatttcc gccagtcggt caccaatccg gccttcatcg gattgtttag aatataccgg
    13621 atcgttcctt caaatttttc ccggtttcga atccaatgat caaactgttc acgcgcccag
    13681 aacggcccgc ttctgccaag aatctgattc actttgtgcg ctgaaaaccg tttgaaatga
    13741 gcaagatcat tcgggagctc gccggagcgc ccgtcctcac tgcgcattaa caggtgaaga
    13801 tgcgttggca tcacaacccc ggcataaatg cgccaaccgc gttctgcgcg gagccagtcc
    13861 agatttttca gaaccatcga ggcgatatca ggatcagcca gccatgctct tcccgattcg
    13921 cacacatcca gaattttttc taatttcaaa aactgtctgc gctgcaaatg aagaagtttc
    13981 ggcgaatccg gcgaggaagc ttccagacta tttcgctttt cctgcaacgt ccgcataacg
    14041 gtttgcggaa gtgttccatg cagacggatg gtaacaaagt atgaccggtc tgacaccagc
    14101 cagtgaggca ggttccgccg gaagaacttc actgtgtttt ccatccttct ctccatctga
    14161 agccggagct acgcgcttcg cgctaagctc aggctttacc aattcccact cataagccga
    14221 acctgaaagt tccggcttta ttaaagtact gccggaactc agccgtaccc ggcgtggatc
    14281 cggcaaccta ttccattaaa catccgagac tgagcagcgc gtaaaaggta tattcgacgt
    14341 cttcgaattc gtccgtcaca tgtccggcga agccgccgct gtcacgccag agagattcga
    14401 taaattccat acacggtctg cggacgggat cgagatccgc ccggagaatc cgcagcgcaa
    14461 aaagcgcggt ggcggtggaa agcaggtcgg cggaattcag ctgatctgtc gcggcgaagc
    14521 cgccggaacg gcagacccgg ttgagcaata ccccggcggc gactttttct gacgtccggt
    14581 tgacgatgac cgccgcggcc agattcggcg tcggatccgc aagtgaaacg ggttgcggcg
    14641 cagccggcag acgggcgatc cccagacagt cgccggtcag ctgtttcagg aaaaaatcat
    14701 atgcggattc cgccggcagc tcttccatgt tccggaaaag ctgccgccgc gttttacccg
    14761 tcatggggaa agctgtgcgc aagcggatca gacagcacag atgaacgaga tcaagctctt
    14821 cgcccaatcc gaacggccgg atatatttcc aaagcggcca caccggaata cgccagccaa
    14881 gagccttcag cccggcggcg gcgaagacgg tgtagtagag atcgctctcc gcgcttttcc
    14941 ctctgaatcc gccgccggga ttctgccggc tgaggatgaa gcgggcgatt tcgctgcgcg
    15001 attcctgttc cagaaaccgg cgggaacagg cggcagtgca aatcaggcgg tcggccagag
    15061 actgcatggc gtcatcctcc gcccagcatt ttagcggcaa cccgccggag cagacttttg
    15121 agcagcgcgt ttttcagcgg attgagcgcg cggacggctt cgtttttata atgctcgagc
    15181 agctgcgccg ctttttcggg aatcatcagc tcatcaaaga aatggctcaa ttgcgcggtt
    15241 tcagcggtca gccggagcga cggcgaatgg tcgttggcca gcgcaagcag cagcgaagcg
    15301 cggaggtcgc gcgcttcgcc ggatttatat tcttcaaggt cgtcgcggat ctgataggcg
    15361 atgccgagcg attcgctgaa agactgaagc gtttcgagca gcgcgcgatc cgccccggcg
    15421 gcgacggcgc cgagcagaag cgccgcgccg aaggccggcg cggttttgcg gcggaaaatt
    15481 tcaatgattt cttttgaggt gaacgtcttg ccgttcgcca gaccgtagag ttcctcgccc
    15541 tgcccgatac agagggtgcg gtggttttcg gcggcgatgc gcagcagtcc ggcggtgcgg
    15601 gtttcaaccg aggcgatcca gcggtagcct tcgccgacca gcaggtcgcc ggcgttgatg
    15661 gcgacggcga ggccgtattg ccggtgaagc gccggttcgc cgtagcgcag gtcgtcgccg
    15721 tcttcgatat cgtcgtgaat aagcgaggct ttgtggaagc attcgacggc gatagccagg
    15781 cggcggactt cgtcgggcag ttcggtcgtg ccgccgttga gcgcgctgta ggtgcaggcc
    15841 agcagatagg gccgccagcg tttgccgtct ttgagcagcc actcgcaggc gatctgtccg
    15901 gtacgggttt ctgtattcca atcgccggaa gcggagaacc atccgtcgac gacttcgcgc
    15961 agacggacag tatcaagcgt gtttttccag ggtgtggagg atttcagcct gatcgcatcg
    16021 cggacatgct cgacgtcgat ttccgttccg gcgcagccgt cgacggtcag cggaatcgcg
    16081 agaccgggaa tggcgccgtc cgccatgcgc gaaaacgatt tttccagcgc ggagagacag
    16141 ctgacgccga cgaccgcatc cactttgccg ccttccagca gtctggaaac gaccgtcgaa
    16201 ccttccgcga tcagtacgac gtagcccagt tcttcggcca gcgcctgaag tccgccgata
    16261 ttgcaccgtc cgcactcttc gcagagcagg ccgaactcat cgggtttggc cggacattct
    16321 ttgacgtcgc gcaggcattg cggaagaagg agcaggcggc gttcgtacgg aatggcggcg
    16381 acggtgtcgc gccagacttc gttgttaagc agcaccatca catagtcgat cagccccgga
    16441 tgtgcggcga tgtttccggc atggtcgcgc agttcttcca tcgagagcgg cggcaccagc
    16501 tgatgttccg cggcgtactg agaaacagca agcagaacct gctcgcgttc gaaccggtcc
    16561 gccggaaccc cgtattttgc cgccgtttgt ttcatcaccc gtacgctccg aaaccgaaaa
    16621 accagtgccg ctttgcaaaa cggtaggccc ggctgcgctc cggaatttct tcgccgggct
    16681 gatgatggct gccgcaggaa cacgtccggc caagctcggc cagcagtgtg ccgcgtccgg
    16741 agctgtcgac cgcgcccgtg cttcggataa agtccgcgag ctgaccgggg tgctccatga
    16801 tgatgcagcc ctgcgtggtt ttgcagcaca tcctgcggaa gtcggcgagg aatttggaac
    16861 ggttgaaaag gtcgtaaagt ccggttccgt cgccgatgtt gtccctggcg aactgaagcg
    16921 gcggacacgg ttcgatgtat ccgtcgggac tgacgtgatt ggcgatgccg accgccgccg
    16981 ggcagagcgc tctgccctga tcgtcccagt aggcgtcaac caccaccagc ggcgctgtga
    17041 ggcggatatc aacgatgaag cggcgcaggc gggtgatctg ttccgcgtcg agcgcgagtt
    17101 ccggcgacgg attcgggccg acggggcggt agatgtaata ccagagatag tgcgcgccga
    17161 gcgcggcggc atcgttcaca aattttccgg tggcgaggtc gtcgatgttg gatttgcaga
    17221 cgctggtgca gacgccggtg acgagccggt tcgcgcggca gttggccagt cccgccagcg
    17281 tctgttcata cactttgttt ccgccgcggc gcacgtcgct gacttcttca agcccttcga
    17341 tgctgatcag cggactgatg tttccgatgc gccgcatttc tttcgcgacg gcatcggtga
    17401 tcatcgtgcc gttggtgaag agcaggaaat aacagtcggg atgtttttcg aacaccttga
    17461 agaggccgtc atagagcagc ggctcgccgc cgaggatacc gaagaagcag gagccctgcg
    17521 ccttgcattc cgtaatgacg ttatcgagtg tgccgggcgc gatctgtctg ggcggattgg
    17581 tttgcgacac ccagcacccc tggcaggcga ggttgcaggc gttggtgacc gacagaaaca
    17641 gaaacgccgg aaaatattcg ccgcgcttca ggcgcttctg gaatttttcg atggagcgca
    17701 ttcctttgaa gccgaagttg tagatgaact tccacagcag ccgcttgtcc ggctcggtca
    17761 gcatccgctt catcagagtc gcgctcatgc cttgtcctcc gaacatttct tccgttccgc
    17821 cagcgcctgt tcgactttct tctgatcgag cagcgagcgg cgcttctgct tgcgggcatt
    17881 cagcgcggcg taaacatcgc cgccgagaat ttcatcgacg ttgattctga tgttcgcttt
    17941 gagcgcttcc tcgtcggaaa tctccgcccc gttgatcggc gcttcgccaa ccttcgggaa
    18001 aatgatcgcg gcggcgggac agatccgcgc gcaggccggg cagttgtttt tgcagctgtg
    18061 cggattcact acgcggattt tcccggcgga atccttttcg tagacaccga acaggcagaa
    18121 ctcgaaacac tgcccgcagt tcgtgcagcg gtcttcgtcc agaaccgggt accaggcggt
    18181 atcggattca gcggacgggc cgccggacgg aatgcgaagg ttgataaatt ctgtattttg
    18241 cggaagcgga aagccggcaa aggcgaacag cgcgcggact gcgcgcggat ggcaggcgca
    18301 aacaaccggc gcatccgcac cggcgatttg tccgagaacc gggtcttttg cggccgccgc
    18361 cccgcagaga tcggataccg ggatcgcccc gtcctgccgc aaaagcggtt cgagcgcctc
    18421 ccggttgagc aggcggcgcc cggcgcagcg gcagatcagg agtgtcttat ttgctgacat
    18481 cgatcgcgac gagcgttccc ttgtcattgc ggcagtaaat gatgccgttc gccagtaccg
    18541 gtgcggtcca gcagagcggg ccgattccgg tgtccagttt cgaaatctcg ttatatttct
    18601 tggcggagac gtccacgaag ctgagcgatc cgtcttcgtc gagtacgatc agtctgccgt
    18661 cggcggccat cagcgagccg aagctgaccg gcgcgctcca cttctcggaa ccgtcggcgg
    18721 cggaaacaca gcgcaggacg cttttccctt tcgcctgtcc gtcgacaccg taaataaagc
    18781 cgtcgataca aacacagctg ccgaaatgcg ctttgatcag gctgttttcc caaaccggtt
    18841 tcagcgcgcc ggaggaaaag tcgagcatcg cgcagccatg atcgtaaccg gaggaaatga
    18901 aaatcctgtc gccgatgaca aggggatccg ctccgttaat cttgtatttg gtttcccagt
    18961 cgaaggagct gatttttttc ccggtcaggg cttcgacgat ctgcaggccg gacgccgaga
    19021 agatcgcggc gcaacgtgtt ccgttgcggt caaagagaac gggtgaggca tagctcgccg
    19081 cgccctcact cttccatttg attttcccgc tgtttttgtc gacagccatt ccggtttcgc
    19141 cggcgttgag cagaatcaga tcctcttcga tgaccggaga agaagacagg ccccatttga
    19201 tgttctgact gccggatccg gcgagcacat ccgttttcca cttcaccttg ccggaaccgg
    19261 cgtcgaagca gatcagctgg ccgtcacggc tgagggtata aacagaatct gcatcgcaca
    19321 ccggagttgc ccgcgggcct ttataaggtc ccggctggca ggaataggaa taggaccaga
    19381 tttcatttcc ggttttggca tcaagacagt agaccgtgtc ggtcttctca tcgccggact
    19441 tcagacccat cgtgtagagc ctgccgcctt taacactcac ggaggaataa ccgtcgccca
    19501 gctccttcgt ccagagtttt ttggcgttct tcgggttcca gccggtttct tttgaaattc
    19561 cgttacggtc cgggccgcgc cagaccggcc agtcgttgca atatccgccg gcagcgatcg
    19621 cggcaaccat caaactgagc atccattttc ccatagaatc ctcctgaccg ctctgcggcg
    19681 gctattgtgc gtcaagaaga ccaaattcag cagtttttta cactccttaa cttcctgaat
    19741 tttgggaaga acccttttgc gctattcatc tttccggtgg caatcgaacc cgatctgccg
    19801 gtttgacggc tccggcggcg gagccagcag cggctggagc tgttcccaga tcacaccaag
    19861 cgcccggtcg tgttccagca gggttttatc gatttccgcc agccgcttga gaatcgccgc
    19921 attagcggcc agctgttcgc gcatccggac aaacgcccga accacaaaca cgctcatcgc
    19981 caccgcttcc ggactgttca gcacggtcgc cgccataatc gcgccgtgtt cggtaaaagc
    20041 ggcaggaagc tgatgagaaa acttaacctg cacgaggtgg tcacaatttg tgaccacctc
    20101 ttctttttca tcctcggtca gccggaacat aaaatcctcc gggaaccggt cgatattgcg
    20161 tttaaccgcc tggttcagcg ctttggtcga tacgccgtaa atttccgcga gatcggcgtc
    20221 cagaattacc ttctggttgc gcagggtcag aatcagaggt tcaatcagtt tcagttcgtt
    20281 catgtcaggc cgtctccgta aagcgtcagg aaagcttctg acatatcttt acaagccttg
    20341 gaggctgaaa aggcggccgg ctaccagaac cagtaggcgc cggtatggac ggcgtaccag
    20401 ccgagcaggc cgttggtgaa agcgtgcgcc caaatcgcgg cccagatgtc gcgggttttc
    20461 accatcagcc aggtgaaaaa caggccgcag agaaagccca tcagccattc gttgtgttcg
    20521 acagcgaaga gcgcggcgac caggatgcac attttcgagt ccatcacgcc ggggtctttc
    20581 ctgaaaaacg ggctgccctg catccagcgg tagacaaagc cgcggaacat gaactcttcg
    20641 atggcgccga tcaccagcgt cgtgccgagc atgtgaatcc agaagagcgg ccagccggta
    20701 gcgcgcgggt cgtagtgata aaggccgttc gcgcccagct ccagcggttc gcgcagcttg
    20761 ccgaacttgg tcagcccgac cagatagcgc tcgtagaatt cgctgatttt cgggaaggcg
    20821 cttttgaaaa attccgattc gagcccgacc cagaacacaa acaccagcag gccgaccgcc
    20881 agcgcgagcg gaacgttttt gagctgcggc ggcgtgtacc agcgccacgg ccggcagatc
    20941 agcaggagaa taatgccgcc ggcggtctga acggcatagc gcgttccgga cggatcgccc
    21001 agcagcgaca tcatcagcag ccaggccata aacggaataa cgtgcgccca gacggcccgg
    21061 tcgttttcct tatccagctc cggcgtccaa tcggctttgg ttttggcaga catgggcgga
    21121 aactaccgga cgcctttgcg gcagaaaagt aaaaacgccc ggggccgtca gttaataaac
    21181 aggatggtgg cgttcagcgc gccggcaaac gaaatccaga tgaaataggg tagcatcagc
    21241 agcgccgccg caaaactgcg cagactgaac agcgcgatac agagcagaat ctggaaccag
    21301 agcagaatca gaaccgccag cgccagatcc ggacggtgca gtccgaaaaa caccggagtc
    21361 cacagcgcgt tcagaaccag ctgggcgcca tagacggaga attccgcgcg gcggtgctcg
    21421 cgcggtccgc gcgtccacgc aaaatagccc gccagaccga taatcaaata aagcgccgac
    21481 cagaccgggc cgaacacggc ggccggcgga ttccactccg gcttatacag cgcttcgtac
    21541 cagccgccgg tctgcacaaa aaccgcgctc agcgccggaa gaaatgcaat cccgacaaac
    21601 aaaacaacct gtcctgcccg cttcagcatt ttcatgcgcg gagtctatcc gagacattgg
    21661 gccggagcaa aaagaaagca accccgcctt tgcgtcttcc acccccagcg gccgttcccc
    21721 cacgcccata cgcggcacgg ttccggcttt tgccgaggcc gatgaatcgg tatgccgcga
    21781 ggaactgacg accgcggttg ttccgcgctg aggcccttcc gcagcaaaga cggcccgaca
    21841 taaaacagag tacaggccgt aatagcaggt agatctgttt cttatttctt tattcagtgg
    21901 gcgctttctg aaaaaccgcg atttccgaaa aaccggcgtt catggttttc ggtccaaccc
    21961 gggtgataat gacctccatc cacttgacga ttttttccgg aaaattcagc tggaaaggtg
    22021 ccgtgccgtc gttaggcagt tcgccgacca tcgcgctggt gccatcgctg aaattaatcc
    22081 acgcagccag cacctgatcc ttttcattag gccggtcaaa cagccagaca tcttcgatct
    22141 tcaccggttt ggcccaggtc agtttcactt ttgcgccggt ggtctcctga ttactggtcc
    22201 attccgccgc cggattgtcg ggaagccctt gcgccacgcc gtccacaaga gcttccggca
    22261 gagaactgct tgctgaggta gatgctgcaa ccttggcgga gcgcgccaga ttcagatcac
    22321 ttttcagcgc ttgcgcctga gcttcgagcg ccttggcgcc tttcacttgc ggaaaggcga
    22381 cgcgccgaag caggttcacg cccatcggct gaagttccac cggctcgctc tggccggttt
    22441 tccggttgat caggcggccg gtcagtcgca ccggcgcagt gatccatggg ttgccttgca
    22501 ctgcgctttg aacacagttg aacgcttcgc ctttttcctc tgcggcgtag tcccagaagg
    22561 ctccggcctt gggcgtgtat tcataatccc caaaaccggc caccggatag gtcttgatct
    22621 gtttgctttc ggagggaatc ggcagcgcat acaccagcgg accgcgtttc cagtacactt
    22681 cgccgttggc catcgtcttg cgttcgacat ccggcttaaa cgaaacggta atatggtcgc
    22741 ctttcttcca ctctttggtg agtacgcgcc agccgctgtc atccaccatg gtcgcgcccg
    22801 ccgcgacaac attcatactg cccgcccagg cgggaattcg cagacggata ctgcaagcga
    22861 ccggcttctc cggcgtgacc gtcatccgta tttcgtcttc gaacggatag ccggtgtcag
    22921 acgtgatttg aatctttacg tccttgatgc tggtccgaag ctcgttcggc gcatagttca
    22981 ccgccaccag accgcggccg tcggcggttt tcatccacag cccgtcggta aaatagggga
    23041 agaagcacag ggcgccgacc gagcagcagg cggccacttc ttcgtgggtc ggcgagagct
    23101 tcatgcgtcc gccgttcttt ttggagaccg ctgcgtattg gttatccact gaacagtatt
    23161 gattgccttt gccatcccgc aaccgtgcgc cttgcgcgta attaaacgcc agcacctcag
    23221 cggcatcggc cgcttttaca tcccgggtct tttccacgcc ggattgtaac gtgaagacca
    23281 gaaatccctg cgtacagtat tcgctgccga tatacggact gccggcccgt tcaagaatat
    23341 cttcatcact gatgcacgcg ccgcccgcgt tttgatggcg cgacagttta agtaaaaagt
    23401 tgtctgccgc cgcccggtat ttcgcctcgc cggttgtata ggccaggaaa agcggaacgc
    23461 gcatatgctc caccgtatgt gcgccgtggc cgttcagcgg cttgttcggg tcggaaagat
    23521 tcttcagcag cgaatcgaac tcgcggacct ccggtatagc gttatagccg tcgtacataa
    23581 acttggcgaa cgccacatag ctccggtcgc cggtgagccg gtagagctct tcgcaaacgt
    23641 ccacaaacat caggctgtgc ggcggaccgc cgcgcccgat gctgccgggc cagtacggcc
    23701 gatccggacc gtattttgac atcaccagct gcgtcgcgcg ctgcacggcg ctgagtacat
    23761 cttcccgccc ggtcagctca tagtaggcca gcagtccgcg gtaaagacac gcctgtgtcc
    23821 acaagtcacc gttctgtacg tgaatgggcg actggaaccg ttcagctttc ggcataacac
    23881 cgagatagcc gtccgcttcc tgcatcgcga gaatctgcgt gacgagttca tccgccctgc
    23941 gcttggcggg ttcatcgccg gagagatagg cggcgcgaac caatccgtcg agccagacgc
    24001 cggttgtttc gccgggccac caggttttat ccggtgtcgt ttttttcgcg ctgtcgaaaa
    24061 acgtcggcag aatatgcttc ggcgtcagct tatccatcac cggcccgtag ccgttggtca
    24121 tatccatgcg gatctgctcc agcagccagc cgcgcggctg cacttcgccg acgtggagga
    24181 atttaaattg atctgccggg gcctcagcgg cgaaaagaga acgggttgcg gcggtaaaaa
    24241 atgtgacgag taataaccgg atcggtgcgt tcatgcggga tctcctttga aaacggactg
    24301 tatcaacggc ctgcacccta tcaatcctga acgaagacgg acatagctga aatgaacatt
    24361 cttcatgccg attccgatca ttctgcgctg accggcggaa aggcgcgaca ccggcaccgt
    24421 cctgtgctac atcctcccgc catgccgatc ctcctgaaaa accaaaccgc cgacgaactg
    24481 caccgccagc tgggcatcac cgcccggcag gcgcgcatga ttcaggccgt cgtcatccgg
    24541 aaaggccaac tgccggaggt gcaggccgag atttctgaaa aggcactgaa agctctccgg
    24601 gccgcaacag agattcccaa cctgaccgtt tcagataaac aggtgtcgcc gcaggacggc
    24661 ttcgccaaat atctctttcg cggcgacgga cccgaaccct tcgaagccgt ccgcatcccg
    24721 ctgctgcacc gcgaggaaga tcagaaatat atcgtctgcg tcagctcgca ggtcggctgc
    24781 gccatgggct gctccttctg ctacaccgga aaaatgggct tcatccgcaa tctctccgcg
    24841 tgggaaatgg tcgatcaggt gttgaaaatc cgcgacgact cagaccatcc ggtgcgcggc
    24901 gtcgtcttca tgggcatggg cgaaccgctg ctcaactacg acgaggtcat ccgcgccgcg
    24961 cgcattctct ccgaaccgtg cggcggcgcc atctccgcca aagccatcac cgtttccacc
    25021 tccggcatcg cccccgccat ccgccgcttc accgccgaaa aacagccgtt ccggctggtc
    25081 gtctcgctca cctccgccga tccgcaaaag cggcgcgacc tcatgccggt cgaaggattg
    25141 cacccgaccg ccgaactgat ggacgcgctc cgcgaacgcc acgccgtcac cggcgaacgc
    25201 accacgctgg cgtggaccat gatttccggc ctcaacaccg gcgaagaaga tgccaaacag
    25261 ctggccgaac tgacccgcgg actgccggtc aaactcgacc tgattgacgt aaacgacccg
    25321 tctggaaaat atattccgcc gtcaccggaa gaactgcacg cgttccgcga cgccctgcgc
    25381 ctgcacctcg ccgcgcccgt cgcccgccgt tacagcggcg ggaaggatat tggtgccgcc
    25441 tgcggcctgc tcgccggtca gaagtaagta aagccggaac ttagagcgaa gctcgtagat
    25501 ccggcttacg ggcggaccaa atagatgagc ttgccggatc cacgccgggt acggctgagt
    25561 tccggcagta ctttgagaaa ccatgcgttc cgcgacgcct tacgcctgca cctcgccgcg
    25621 cccgttgtcc gccgttacag cggcggtaag gatatcgggg ccgcctgcgg cctgctcgcc
    25681 ggaacacttc gctaacatca aaatttcggg aagacaccgg ggtgagaatt ttccggctga
    25741 aatacttatg tctttccgcc gaagaaaaac accatcttgt cctttggccg aacaaaaatt
    25801 atttccgccg ccgatggcct gacgccatga caatagatgc tccacgagta gatatagata
    25861 tcgccggatc gcaactcgtc ctctgagggg aaccacggaa tctcgtgcag ccaaccacca
    25921 ataaaggaaa ccgcgtcatc gagaactttg ggtagctggt cgcctcgata ccagcggcct
    25981 ctgacctcat cggtgctggc ggtcagctcc tcaaccgtca cgctgctgat tttaatcgca
    26041 tacgcatcga ggtggtcgcc ggtaaatgat cgggaagaaa cgtagctatg aaccacgctc
    26101 tcggtccgac tgtcgcccag accgcaccag ttcaggacga accgcacatc gtgaggcgac
    26161 gcctgatcgg cttttgttcg attgcccacg ctgagactgt aggcaatcac acccgccaga
    26221 cctatggcgg caaccaagga taaaacgccc catgagagaa acagccactt tgcaatcttt
    26281 agtattcgtt tcattttccc ctgccggacg gtgttattac gcacttccgt tttaacgggt
    26341 taaacaaccg actccgcgaa tgtagccgga cgggttgtgc acagcaaact ggaaatggct
    26401 ggctggcgcg cacgccggag aattgtagga caggcttccc cggtattccg gggagcctgt
    26461 gtttttaaat caccacacgc tcccgaatac gggaagcgcg tcctacaatt gggtggaacg
    26521 cgccgtcccg gcgcgtttcc tcctgaggca gacaaataaa gcgtgcgcgg agcgcccgct
    26581 ccacctttca aagtggggca ggctttccag cctgcccatg cacaggctgg aaagcctgcg
    26641 ccgcatcttg aaggttgcga atcagaacca aaacgtggac actcccggcg cttaaaccta
    26701 tgaatcctca atcgaaaatc gaaaatcata aatcatcaat cgaactgatg tctcccgcgg
    26761 gctccgaggc ggcgctggca gcggcgatcc gcgccggagc ggattccgtt tatttcggcg
    26821 tcggcaaact caatatgcgc gcccgttccg ccggtctggc gccggaagac ctgccgaaga
    26881 ttgcgcgccg ctgccgcacg gcgggcgtga agagctacct cgcgctcaat acgattgtgt
    26941 acgacggcga gatggaggag atgcatgcgc tgtgcgacgc cgccaaagcc gccggtgttt
    27001 ccgccgtgat cgccaccgat atcgcgacga ttgaatatgc ccgnnnnnnn nnnnnnnnnn
    27061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27121 nnnnnnnnnn nnnnnnnnnn nnntacatga gcctcgcgca gtacgaccac tcggccaacc
    27181 gcggcgcctg ttttcagccc tgccgccgca gttaccggat tacggacgag gaaaccggcg
    27241 cggagctggt gatcgacaat aagtatgtga tgtcgccgaa agatatctgc acgatccagt
    27301 atctggacaa aatcaccgcc gccggaattt cggtgctgaa aatcgaaggg cgcgggcgtt
    27361 ccgccgatta tgttgaggtg acgacgcggg tttaccgcga agcgctcgac gcgctggcgg
    27421 ccggaaccta tacgccggat aaattggatc cgtgggtcgc cgagctcggc aaggtgttca
    27481 accgcggttt ctggcacggc ggctattatt gcggcacgaa gctcggcgag tgggcgggcg
    27541 cggcggattc gcaggcgacc gagcagcgcg acgaggtcgg cgttctcaac aatttttacg
    27601 gcaaaggcaa tatcgccgag ttcacgctgc ggcgccattc gatcaaaacc ggcgattcgc
    27661 ttctgatcga agggccgacg accggcgcgc tgcggtttac ggtcgagggc ttgcgcgtca
    27721 acggcgaacc gtcggagtcc gctcagccga acgatgccgt cacgctcgcc ctgccccgta
    27781 aagcccgccg gcaggataag gtttttctgc tcacgctgcg cgggtagaac cttcgaaata
    27841 ctcctttctt ccgggacggt tctgcatcaa aatatagccg acgggagaag cagagaatcg
    27901 aactccggac ttccgccaga ggaaggagga cactatgagc agaacgattg gactctggat
    27961 tgatcataag gaagcggttc tggtttcgat caaggacgga gcattgtcag tccgccgcgt
    28021 ggaatccgaa gtcggaaccc gcttcagtcc gtccggcgga tggaaggccg gcggaacccc
    28081 ggtggcgcaa tcaatcgcca aagaacagac ggcagatgag cgccgcaaac acgggcttcg
    28141 tgcctattat caggaactga tcaaagccgt cggcggcgcc gaagagcttt tcattttcgg
    28201 ccccggcgaa gcaaaaaatg aactggttaa ggaaattcaa aaaataaagg gctcgcctgt
    28261 ccggatcgcc gccgttgaaa cgtgcgacaa gatgaccgaa ccgcagatcg ccgccagggt
    28321 gcggaagttt ttcaaggtct gaagtgacgt gtctgaccgc aaccggacaa aagagctggc
    28381 aatcggctgg cggcaacttt atggtctgcc cctaacaaag gggtttttat gcgtataaca
    28441 tctgttccgg ttgtgcttct tctcgcgctt tcgttcgcat tctgcggatg cggaaaaaaa
    28501 ccgcagccgg cctctgtgcc gctcccgacg gccgaagcgg ttgccgcgta tgcggaagcc
    28561 gctttcaacg gaaatgcccg gcaggtcgcg gctgcgcttc agaacggcat gccggtcgat
    28621 caaagggata aagacggcaa ttccgggctg atgctggcct cttttaacgg ttctgtggaa
    28681 accatgcagc ttctgctgaa tgccaaagcc gcagtcaatc tgcgcgataa caaaggacgc
    28741 acggcactga tgtttgccgc ctccggcccc tttccggagg cggtccgcct gcttctcgat
    28801 aaaggtgcag acgtgaacgc tgtggatgcc gtcgatcatt tcagcgcgct gatgttcgcc
    28861 gcggccgaag ggctggatcc ggtggtggat attctgctca agcacggcgc cgatccgaaa
    28921 atgaaggata aggataacga cacggccgca aaatttgcca gagaccgcgg attcaccggt
    28981 ctggccgaaa aacttcaggc gctgatcgac gcgccgtaac acggagaatt ccatgaccga
    29041 ggatcagctt tcagatgccg gatcgtataa gttcggccgg ttccagaaaa atgccgagga
    29101 acttgcccgc ctgaagatgc aggcaacggt agccattgat atcgagcggg aggtttggaa
    29161 aaacgcgggc ttaaaacccg gcatgaacgt tctcgatctt gcctgcggtc ccggattcat
    29221 cagctgcgaa ctggcccgga tggccggaac tgtgaccggc atagacatca gcgaagagct
    29281 gattttcacc gcgcaccagg cgaaagtgtc cgaaaaagcc gataacgttt cgttcctgac
    29341 cggcaatgtc tatgatctcg ctctgccgga aaacgcgttt gattttgttt acgcccgctt
    29401 tcttttccag catctggaaa aaccgcagct tgccctcagc aatattctgc gggtcctgaa
    29461 accgggcggc attctgtgtg tgctggatat tgacgataac tggaccagct tcgcgccgga
    29521 aagcgccgct ttcgtcaaat tcatccgcaa ggccggcgcc ggacagaagc gcaaaggcgg
    29581 caaccggctg atcggttcgc agcttttcgg cctgctgaac ggggccggat tcaaagaagt
    29641 ctccgtcaaa atccagccga tcaccaccga agacctcggt gtaaaaatgt ttctcggcgt
    29701 agccgttctt ttccggctgg aaatgctcaa taaactgcaa aaactgctcg ctctgcctca
    29761 gctcaaaaaa attaaatccg ccgcgcaggc tccgaatgca tggggctcgg tcggcgtttt
    29821 tatcgcaaca ggcacgaaag cctgatccta tgcggtttaa aggcaacagc gaccaaagca
    29881 gcgcgcgcta ctgggacgac cttgcggaac tctatcagcg agaaacccgc atttcgacgg
    29941 acgacttcca ctacggcccg ctcctgcccg gcgattcaac cctccgtctg cttccggtcg
    30001 ccggcggcct gcgggcgctg gagatcggct gcggcgcggg tcagaattcc atcttcctcg
    30061 ccaagcaggg cgcacagtgt gtcgccatcg atatttctga agcgcagctg gcgcatggac
    30121 gcaagctggc ggccgccgaa aacgtcagcg tcgacttccg ccggatttcg atggatgcga
    30181 tgggcgacct cggaaaattc gacctgattc attcgtcgta cgcactgcct ttttccgccg
    30241 atccgcaaaa agtaatcgcg gcggttgccg cgcggcttaa acccggcggc acttttctgc
    30301 tgacgaccgg tcatccgctc tacgccggag aatggctcga tatcggcgac ggtgaagacg
    30361 gcctcttcct gcccgactat ttccggccgg agcccgacgt gcggatgtcg ctggatgaca
    30421 agaccatgag cgccgcgcac tactggccga tttcgaccct caccgaatgg attcactccg
    30481 ccggactgac aatcgtccgg ctgctggagc cggcgccgat gccgatcccc gaaatgagcg
    30541 aggcggaaat ccgcgccaac gtgccatatg acagccccgg ctggcgcgaa ctctattccc
    30601 agctggcccg gattcccgtt gtgttggtac tgaaatgctc gctaataaat tgacttagat
    30661 tggggctgac gacactattt tagcccgtaa agcgggctcc agatagatgg aggccgggtt
    30721 aacgttaact accgaaaggg aaaaaaatga aaaaagtact tctgttcagc atgattgcca
    30781 tcgcaggcct gaccgtaagc gctgtcgaca tgaccaaaga ggtctacatc gccaagatca
    30841 aagcagttgc cgagagaaaa ggccgggcat ttgatgcgga aaaagtcgcc gcagacttcg
    30901 ataagctcga caaaaataaa gacggcgtct ggtctgatga agaacagggc aaggttcccg
    30961 ccaagccaaa agatcccgca gcgacacccg cagctgaata aagttgctta gcgttacaaa
    31021 tcgcgccgaa tccggttcga ttcgtatgtt tcaaaaagcc ccgctaatcc ggcggggttt
    31081 tttgtttata cggttcagat aaatcagatt ccggcattca atttgaaaca gttctttctt
    31141 cgaagcgcga agcggttggt atttttattg caacaaacat aacagcttga tcctatgcga
    31201 cttaaaaaac atgagaccca agctgcgcgc tactggacga tcttgaccag tgcggatatg
    31261 gatttactcc gccggactga caatcatccg gctgctggag ccggcaccca tatgaaagtg
    31321 ccggccggcg cgaactctat tcccagctgg cccggattcc cgttgtgttg gtactgaagt
    31381 gctcactaat aaattgactt atattggggc tgacggcatt atctcacatc gtaaagtggg
    31441 ctccagatag atggaggctg ggttaacgtt aactaccgaa gggaaaaaaa tgaaaaaagt
    31501 acttctgttc agcatgattg ccatcgtagg cctcaccgta agcgccgccg aaacgactaa
    31561 agcagactat attgccaagg caaaagcagc ggcagagaaa aaaggcagag cttttgatga
    31621 agcaaaaacc gcaaaagcct tcgataagct ggacacaaat aaagacggcg ttctgtcggc
    31681 cgaagagcag gccgctccta aagctaaata aggtgacata acgaatcgcg ccgaatccgg
    31741 cgtgcttcgt actgtttcaa aaacccgccc tccggcgggt ttttttgttt tatacgcccg
    31801 ttgcacagga gcatttcgga gtttcaaatc tgttcgatgc agctatactc cgcgaaatgg
    31861 caaaacaatc taagcaatcc gaaattgtcc gttccccgaa acgcgaagcg gtcggcgtca
    31921 ttcttttggc tctctgtctg ctcagctttc tctgtgtttt ttctttcaac cccgaagata
    31981 tcggctggct gcataatccg ccggcatctc cgccgcataa tctggtcggc ccgttcggag
    32041 cctggttcgc cttcatcggc ttcctgcttt tcggtgtcgg cggatatctg cttccctttc
    32101 ttctgctgac gggcggcctg atgatgacct tccgctcgta cgagcgcggc tggcctgtct
    32161 gggcctggct ggtcgccgga ttgagttcgc tttcgagcct ttttgaatat cagcccggtc
    32221 tgtgggccgg attgaaagat atcctgaatc tgggcagccc cggcggactg atcggccagc
    32281 tgctggccat ccgtacggtc ggaaagctga tcaaccatac cggggcggga attgttttcg
    32341 ttaccgtttt cattctcgcc gtcctgcagc tgaccggcat gcacccgttc actccgttta
    32401 ccttcctctg ggggaaaatc caggagttca gagccgcccg caaagaagcg cgcgccgcgg
    32461 caccgaaaac caaattcagc cggcaggtgc cgcctgctga aaaagaaaag ccgaagaaag
    32521 ccgttctgtc gacgcccgaa cccgcgccgg ctgagggcga actttttgtg ccgccggtct
    32581 cgataccgaa agccgaagcc aaagcggaga ccaaaccgga accggtcaag cctcagccca
    32641 aagttaaacc ggttgcgccg ccgcccgccg acgaactgtc caacgtttcc gtcaatatcg
    32701 caccgggccg cgacgaatcc ggatatcagc ttccgccgtt gacgatcctt gacgagctgc
    32761 cttcgctggc ttcccgcaca tccaccgaaa acaccgagga aaaggcaaaa attctccagg
    32821 ccacactcga agagttcgac gtgcagggtc aggtgaccca tgttgaaacc ggcccgaccg
    32881 tgacttccta tgaaatcacg ccggctcccg gcgttcgggt ggaacgcatt tccgcactgg
    32941 aacgcaatat tgcgctggcg ctccgcgctc agagcatccg tattcaggcg ccgattcccg
    33001 gcaaaggcgc agtcggtatc gaagtgccga actccaagag tacagctgtt tatctgcgtg
    33061 aactggctga gagcccggct ttccacgatc ccggcatcgc cctgccgctt gcgctcggca
    33121 aagatgtcag cggccgcatg gtcgtcggcg atctttccgc catgccgcat atgctgattg
    33181 ccggagccac cggcgcgggc aaaaccgtct gcatgaactc gctgctgacc ggcctgctgc
    33241 tttcgcgtac gccggaacag ctccgcctga ttctggtcga cccgaaaacc ttcgagtttc
    33301 aggcctatga aaacctcaag catctggtcg taccgattat taccgacgcc aagaaggtga
    33361 cactcggact gcgctgggcg atcaaggaaa tggaaaaacg ctacgccctc ttcaaaaagg
    33421 tcaaagtgcg cgacatcaag ggcttcaaca cccgtccgat cgaaaagcag accgacctgt
    33481 tcggtgcggc cgccgaagaa gacgatcagc cgaaagatgt ggttccggcc aaactgccct
    33541 acatcgtcat cgtcgtggac gagctggccg aactgatgag cgtcgcgcag caggaaatcg
    33601 aaccttgtat tgtgcgcctc gcccagcttt cgcgtgcggc aggcattcac atgattctcg
    33661 ctacgcagcg cccgtccgtc aacgtcatca ccggcaccat caaggccaac attccgggcc
    33721 gtattgcttt tcaggtggcg cagggcaacg acagccgcac cattcttgac accatcggcg
    33781 ccgaaaaact gctcggcaaa ggcgatatgc tttatctgcc gcccggaaaa aacaaaccgg
    33841 tccggcttca gggaacctgg acgagcgacg gcgaaatgca ccgcgtcacc gacttcatcc
    33901 gcacgcaggg catgccggaa ttcattactg aaatcaaaca gaagattgaa aacaaccggc
    33961 cggaactgcc ggatatggac gaaagcgacg acctgcttga atccgcagtt gaagtgctcc
    34021 gcagcaccaa gcgcgcctcg acctcttcgc tccagcgccg cctgaaaatc ggctataacc
    34081 gcgccgcccg gctgatggat attctcgagg aaaaaggcat catcggcccg ccgaccgaaa
    34141 ccggcccgcg cgatattctg atcgacctcg acggcgaaat ccccgaacac ggcggaagct
    34201 ctgaaccgct ttccgaagca gaggccccgc aggaatttct ggacgacgaa gaggagcata
    34261 aaccggagga ttaaccgcca tggcaacact cggacagcag ttgaaagccg cacgcgaggc
    34321 caaaggaatc agcgaatcag aagccggtgc cgcgacaaag attctgaccc ggattattgt
    34381 cgctatggaa gccgacgatt tctccggcat ggcggctccg acctacgcca aaggattcat
    34441 ccgcctctat gccggatacc tcggtctcaa tccggatccg ctgatcgaag aatatacggc
    34501 ccggcatacg gccgcggcga agccgcttgt tgatgaagca agccggctgg agcagaaccg
    34561 ccgcccgccg ccgtcttttg ccgcgattcg caaacggatt cctgtaaagt tcaatccgcc
    34621 ggcatggctc cgcattccga atttcaccaa gggattaaag ccgtatgtgg tgaaagacct
    34681 ccgcgccctc gccatcggag cggccgcttt gctggtgctg atcgttctgg cggtcagcgt
    34741 ctccaactgc gtccgccggc atggcgctga aaaagccgtg aaacagcccg ccgaggcttc
    34801 cgcccgccgc cttcttgaca aaccgctgcc cgatctctat ctggtcgagc ccggaaaaat
    34861 tgaaagcaaa taacagcaaa ggatgaatta tgaactatga actatgaaaa aggtcttact
    34921 ccattttcat ctttcatctt tcatctctca tatttcacat gaatccacct aatgccctca
    34981 ccgtcagccg gtttgctctg gccgccgttt ttatggtctg cctgagcgtt tcgtttcccg
    35041 gcgcgccgct ggccgccgtc atcgttttcg gcgttgccgc gctgacggat gctctggacg
    35101 ggcacctcgc ccgcaacgtg tacggctgct cctctttcgg caagctgatg gatccgctgg
    35161 ccgataaagt cctgaccgct gcggccttca tcggttttgt cgaactcagg atcatgcccg
    35221 cctggatggt cacactgatc ctttcccgcg aaatgatggt gaccggcctg cgcctgctgg
    35281 cgattgataa aggtcttatt ctttcggccg ggagctgggg taaacacaaa accatctggc
    35341 agatggtctt catcagcgcc gttctgctgc tgcggatggt ttactggatt tatccctgga
    35401 gctttctgga aagcgaactc taccggcaga tcctgctgtg ggccggcgga attgtaaccg
    35461 tactgaccgt ctggagcggc atcatctact tccgccagaa tctgtcgctt ctccgcaatt
    35521 aaattgaaaa agaacgcccg ctctgtaacc tgttccccct atggcaaacg tacgcgaaga
    35581 aagctggcag attaaacgac gctccgacct ctgcgccgga accaacgaac cgttcagcga
    35641 taaagaggaa atcgtcaccc gcctgctttt caaagacggc gaatacgtcc gcgaagatta
    35701 caagctttcg tactggaacg gacatcagcc cgaccacgga ctcagctcgt ggaaaagtgt
    35761 cttccgcgtt ccgccgccgc ccacggaagt cgtgaagaag gaaagcgccg aaacccttct
    35821 gcgcaaactg gtcgccaaag aagatgccga cgacatcaat tccatctaca ttctcgccgt
    35881 catgctcgaa cgcaaacgga ttctggccga gaaagacgtc cagatccgcg acgatcagac
    35941 caaagtgcgc gtgtacgaac ataaaaagag cggcgaaaca tttctggtga tcgacccgga
    36001 actcaaactg gcggaaattg aaaaagtgca ggaagaagtc gtcggcctgc tcggcggaaa
    36061 accgccgaaa accgaaaccg tctatcactt cgaagccatc ctgcacgcgc tgatcgaaaa
    36121 acacgaaaag cttctttttc cgaaacgtcc ggtcggcggc gaagccggca tcgcgcagaa
    36181 actggccgca tctttctttg cgctgctcgc cggaaaaaag aatccttact gggaagaatc
    36241 gaacaaagtg ttcaaccgtt ttgaggattc cgaaacctgg ggcccggccg tcacagccta
    36301 ccgcgatctg gccgaacgca ttccgcagga agtccgcttc ttcggtatgg agaaaccgga
    36361 actcgccaaa cagcttgaag cgctggccat caaagagccg aaagagctca
//
LOCUS       DAOPZO010000004        35555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_1095_length_35555_cov_6.536789, whole genome shotgun sequence.
ACCESSION   DAOPZO010000004 DAOPZO010000000
VERSION     DAOPZO010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 35555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35555
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1095_length_35555_cov_6.536789"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PKI68_00540"
     CDS             complement(<1..611)
                     /locus_tag="PKI68_00540"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNX52210.1"
                     /translation="MIKLRTFQTSEELISQCLATFHEILPTLGTADGVMLAGGKTPLE
                     IYRRAVSDTKGVLFLSDERYVPVADPQSNYGTISPFFPNLLKIQTDRPIEVAARTFDA
                     ELRKLKKIPLGLLGLGADGHTASLFNLKDAALRDERLAIPVMKKEKPDRISVTPHLLH
                     RVQKILILATGADKRTMIQTLLKNPETIPAGVALADHPDVEIWT"
     gene            complement(725..2200)
                     /gene="gltA"
                     /locus_tag="PKI68_00545"
     CDS             complement(725..2200)
                     /gene="gltA"
                     /locus_tag="PKI68_00545"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HNX52211.1"
                     /translation="MSHLTPEQLTEEARQELARLTKPLSPKQRLAIPEQEMPAQDPVA
                     RRGNMQEVALGYSGEQAKLEAERCLQCPTKPCVKGCPVGINIPAFIQKITEGDYQSAI
                     GIIRESSLLPSVCGRVCPQESQCQAPCTVGKALKSIDKAVAIGRLERFVADWERENGL
                     IKSPDVKPATGKKVGVIGSGPASITVAADVRREGHEVTVFEAFHRPGGVLLYGIPEFR
                     LPKAIVDQEISSLKSMGVEIKTDFVIGRTRRLTDLLEKDGYDALFIGTGAGLPKFMNI
                     DGENLVGVFSANEYLTRANLMKGYDNRAATPIFPSKKVAVLGGGNVAMDAARSAVRLG
                     AEEVHLIYRRTEAEMPARVEEVAHAKEEGVIFHTLRNPTRILADERGWVSGMEVLKYE
                     LGEPDSSGRRRPVEIKGSEFEMEVDTVIVAIGNESNPLIRQTTPGLQTSKWGTLIVDD
                     TGKTSLDRVFAGGDIVLGAATVILAMGEGRRAAAAINKLLL"
     gene            complement(2301..3155)
                     /locus_tag="PKI68_00550"
     CDS             complement(2301..3155)
                     /locus_tag="PKI68_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002415046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNX52212.1"
                     /translation="MNRILSKRQLSAEVFQMRIEAPLIARERRAGQFVILQLNSHFGE
                     RIPLTIADADPVEGSITIIFQTVGRTTHLLALKNAGDTVEHLVGPLGQPTHIEKFGRV
                     ICVGGGIGVAPLHPIAQAMKAAGNEVIIIMGARNKELLILEEEMRRIADELIICTDDG
                     SYGRKALVTAPLKELCEADPKPALAVAIGPPVMMKFCAETTRPYGVHTMVSLNTIMVD
                     GTGMCGGCRVTVGKETKFVCVDGPEFDGHLVDFDNMISRLKAYKPREDADHHRCHLEE
                     AIKEKDVV"
     gene            3386..3733
                     /locus_tag="PKI68_00555"
     CDS             3386..3733
                     /locus_tag="PKI68_00555"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNX52213.1"
                     /translation="MTVTMLKSKIHMATLTGTELYYEGSISIDQDLLDAAGILVGEQV
                     HVVNLNNGNRLVTYTIPGKRGSGIVELNGPAARLGYVGDKVVIITYAQMDEAEARTRK
                     PTVILVDEKNAIR"
     gene            complement(4175..4750)
                     /locus_tag="PKI68_00560"
     CDS             complement(4175..4750)
                     /locus_tag="PKI68_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNX52214.1"
                     /translation="MNKILVVMMFMFAICVSVKASDIVWGNNTEIYKASGATDLVLDT
                     DTNYFIRLYKSTDSVIDFSIIGNAISLGADTYTGIQFNWNSAGENGFCIDNIFGSDSS
                     YGINAGDKIYSVIFDNSLSSGNCAIIDDSITTVFYSAGTMNYDAGGVNGGLKPGGDWQ
                     AIPEPATFLLFGIGGIGAWLIRRKQMDKIED"
     gene            complement(4801..5598)
                     /locus_tag="PKI68_00565"
     CDS             complement(4801..5598)
                     /locus_tag="PKI68_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52215.1"
                     /translation="MKKMVAILIAGIAVTASAVTNTVSSANVVGYIQVVDPVSNKYVL
                     ISAPFNSGTGTVSTLLDIFGTNQLRQSQLATRCDLIIMWDEVSQQYVRYGQKTNGLFY
                     LNTAFTGSPTNPIVKRGQAMWLQAAPGTYAPTNKIIYISGDVPNDDSYTNHIVGNSGS
                     PLTFIANPYPVEMDLNSLINTNDGAIASALATRADQVRIWDDATQQYVYYALKASANT
                     NVNNKWLINTSFAATNPPVVKIKPGQGFWYQTTNAFTWVETKPYNLN"
     gene            5974..>7176
                     /locus_tag="PKI68_00570"
     CDS             5974..>7176
                     /locus_tag="PKI68_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNX52216.1"
                     /translation="MNKTDFLRKLPGSDVCYYSLPELEKQGGYDFSRMPFSVKVLLES
                     LLRMQGHPAYTPQHVTQLAQWSPRSELVELPFMPARVLLQDFTGVPCVVDLAALRSAM
                     QKAGKDASRIEPQVPVDLVVDHSVQLDASGCAAALELNVAREFERNGERYKFLKWGQG
                     AFEKLRVLPPGLGICHQINMEYLAACVAFEDGIAYPDTLVGTDSHTTTINCMGVLGWG
                     VGGIEAEAAMLGQPIPILTPEVVGFKFTGALKPGVTATDLALTVTRLLRKKGVVGKFV
                     EFYGEGAANLSLADRCPVANMAPEYGATMGFFAVDEKTIGYLRETGRSEEQCALVEAY
                     CKAQGIWGFESEADYNDTLELDLSTVEPALAGPSRPHQQLSLAGLKEEFQKDFGGQGA
                     GSTEQGGEE"
     assembly_gap    7177..7276
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7277..8647
                     /gene="acnA"
                     /locus_tag="PKI68_00575"
     CDS             <7277..8647
                     /gene="acnA"
                     /locus_tag="PKI68_00575"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HNX52217.1"
                     /translation="ITSCTNTSNPGLMMGAALVAKKAVEKGLTVPAFVKTSLAPGSQA
                     ATRYLEKAGLLEPLNRLGFQVAAYGCATCIGNSGPLAPEIEEEIKAKDLTVAAVLSGN
                     RNFEGRVHPLTKANYLASPALVVAYALAGTVNIDLETEPVGKNASGEAVFLKDIWPTT
                     DELQAAIALAADPAFYTEVYGDAFNISPEWNALETVKSQVFEWDGDSTYIREPTFFEG
                     CGQPPAPLADIQNASVLGLFGDFITTDHISPAGNISKKSPAADYLRAHGVAERDFNSY
                     GSRRGNHEVMMRGTFANIRIKNKMVEGEGGRTVYLPTGDEMFIYDAAMKYAESATPLV
                     ILAGKMYGAGSSRDWAAKGTKLLGVKAVIAESFERIHRSNLVGMGILPCEFINGETAD
                     SLGLTGRESFTITGVADHFVPGKILTVRAGGKEFQVKARVDAPLEIEYLKNGGVLNYV
                     LRNLMA"
     gene            complement(8697..8861)
                     /locus_tag="PKI68_00580"
     CDS             complement(8697..8861)
                     /locus_tag="PKI68_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52218.1"
                     /translation="MTKLLIWVGITLGGWIGWWLGGIMGFGLWGEFFVSGLGSIAGLF
                     IGWKIAQEWF"
     gene            complement(8858..10096)
                     /locus_tag="PKI68_00585"
     CDS             complement(8858..10096)
                     /locus_tag="PKI68_00585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX52219.1"
                     /translation="MRKLHGTIAHVMRRFVPDKWGGTETVVFNLARELEQNGLNSPIF
                     CTDMFARTGAEKIDGVTVRRFRYCFPWFGLSEEAKSALRLKGGSPLSLPLFFALLFQK
                     DLTLIHTHVGRRLGGIARTVARLRRVPYVIHVHGGRHTVPQDQYDQMVAPVEGKREWG
                     KLFGLLFGSRRVFDDAAAVICVGQSEADEMRRRSPGRNIHYLPNGVHVRRFAAAGPDA
                     FRNAYGLENRKFVLCLSRIDFQKNQLLLVRAFAEFRKTHPDWNLVFIGSVSVEEYHRQ
                     IIAEIARLGLNDAVLIIPGLKPDDPLLPSAYKAADLFVLPSANEPFGIVILEAWAAGT
                     PVIATRVGGIPGFTTDGENILLTDDNNDARLTEQMNRLAGSPELRAKLATNGFAEVSA
                     NYDWSAIAERVMKIYEEVLK"
     gene            10199..10654
                     /locus_tag="PKI68_00590"
     CDS             10199..10654
                     /locus_tag="PKI68_00590"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNX52220.1"
                     /translation="MDFDWLIIDGYNLMHQDGAMEGSRSDLPTARQRLVRRIERTATE
                     LASRITVVFDGREGGRDAVFDAPHLEVIFSPSNRTADGLIEQMVHDAKQPERILVITS
                     DWTEQRLVSVFGASVISCREFLLRCEPARTPPKRLKSAPGSKLGDFFPE"
     gene            10659..12002
                     /gene="rimO"
                     /locus_tag="PKI68_00595"
     CDS             10659..12002
                     /gene="rimO"
                     /locus_tag="PKI68_00595"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNX52221.1"
                     /translation="MKNTKKQSRSPVVALVSLGCAKNTVDSELILGRFVESGWMIAEE
                     PADADICLVNTCGFIHDAREEAAGVLHELRGLKNGGGPRAVVAVGCLVKRVADSPELE
                     KFLEAADARVSFPDYSRLPEICAGLLGEKTEASEPEGYSDFLIRPRLRTGSPHTAYLK
                     ISEGCSNPCTFCSIPKIRGKQVSRPMDDIVDEARALMEAGAVEINLIAQDTTAYGKDW
                     DGELHLKTLLCRLRDEIAEDIWFRLMYACPQHLTRDVLETMASDPRFCPYIDMPLQHI
                     SDRMLDAMGRRVTKAQTLEKLDLIRAVLPDCAIRTAFITGHPGETEADFAELLAFIKE
                     GRFTHAGVFTYSAEPGTLSAKLEDGVPPAVKEQRRTLLMEAQREVSAARCAAQVGRTV
                     EVMIDGHGEEGLVSRSQREAPEVDGVVFLPEFDAVPGDRFDVQITGSLDYDLIAE"
     gene            complement(12012..13178)
                     /locus_tag="PKI68_00600"
     CDS             complement(12012..13178)
                     /locus_tag="PKI68_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNX52222.1"
                     /translation="MKIKQFILLPILLTAIITAGCTTLRDKPRLNSNKELQPAEVLEL
                     PNAGDLTLKEMETGVWIQLNPDEQVDTKLLTKPKEGPYLLGVGDVIQITLYGEGDAET
                     RRTLPIDPSGNITYMLLGTIPAAGRTIDDLTADMRKRLNEKLNYSILSVIPVRFSSQT
                     FTVLGQLNKPQVYPLQGRTYILDALAQAGGPKVGVIRDVSADIHDYAHATLVRGGEVI
                     PVDFERLVYKADASQNIEIRSGDIITIPSALDRKIYALGAFNNPQGIQFASSQTTLMN
                     AVVQAGGINRELGDGHVLIIRGNTTNPKVMLMDMSQFDSKKARVFKILSGHALDVTLQ
                     AGDIVYAVPRKFGFCRDVVKQAVTMFGTTITRDAATDIWQRKVDPFNYGNNRPQ"
     gene            complement(13175..14248)
                     /locus_tag="PKI68_00605"
     CDS             complement(13175..14248)
                     /locus_tag="PKI68_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNX52223.1"
                     /translation="MRNKPVITAILLLLAGLTRAAEESAAPVSTDSPITATQKRSKFA
                     PEYFVPHSPEDRKLQVGDEVEIGVFGQRDTIAENVPVAPDGKLYYMFLPGVPAEGRTA
                     QEVAKEMESKLGRLFNNPEVSIIPTRFISNRFAIFGKVESSGIYPLDTPTTVRQSIAK
                     AQGIALGKYHDSWIPIHNYKDSWLLRGQEKIPVNFAALMDKDDFSEDIYVRPGDVLYI
                     ASALGREIYLMGEVNQPQSQPFTAGLTLTQLISGLDAGAGGYKQDTADIKRVVILRDA
                     MNDPKVFKVNLDAIMRGKEHNVFLEAGDIVFVPEKSFLFTRTLVKAMVSTFVNTFASE
                     FASDLNRKYLFPGGFTTPRVTTP"
     gene            complement(14238..16982)
                     /locus_tag="PKI68_00610"
     CDS             complement(14238..16982)
                     /locus_tag="PKI68_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52224.1"
                     /translation="MRDKPSEDLHFGKPAEEKPAVPPVTENKPADKTPEETSGTAGTS
                     AEESAGKRPDQEKEPAPRFGEEPPFRIKDQFSDLSDIPPTLSRDTVRPSKASIPGAGM
                     AETARPGRTIRKENAFQDFFRNLDLIKIVRGIYRKFWIVLLSAFGMMILLIPVARVLQ
                     GGVTWSAQSVFIYTKPTQKHIDTHGSTFLMRPLSQDTLVDMFLSPAHIKTLEDAVGLK
                     PLRKNVSFDSQSKSDIVTLKVTGIPDEKTAVAAVNKLADIIISDNDQYYRQLAASAYE
                     LYKTQREVAEKNFNEAVQSVEAFQLKNKILELNTQYQNYFSSVNAASERLSIAQVAHD
                     GLLVRIKNYEKMISELPEEVLNESLEENPLKRRISNAEAALLDARIQYAADNPKILRQ
                     EREIAELRKLLQSGSYDETRERTYVKNPLKGQLEGELLKLRSEENVAAQQVTALQKDL
                     DDLQLKFQDLPRLEKEYAALLEKRVQTDASLKSLKASEESARLTMNSSLSDFKLISPA
                     TTAEPTAASLIGKIIPIAGFIFGFFGGLLLVLIIELLDAKIRTQQQLENAYSAPCLAS
                     IIEVPNLENYDTYQLLLPSLREISERLNVILQGQRAKVIGFFSSLDGEGKSILSFNLA
                     RYYSSLHIKVLFVGFDAQPNPCLPGAAETGWPQMGIEDYLRGTAELADMVTNINGVDV
                     IRVQELRADLLDLAKSSSMPRLWDLLRQNYDLIITESPAVLDHPISGTIAGFQDEIIY
                     VLASPVSDRRLVDAGLEFLEDRGLAPRAIIFNRVNPYYLEDVRQQRIIRNLAEPRNPL
                     AEILDRLQPLLGLFARFRKPAEPAAGTAQKEQVSGQPFEDETAGFPEDEDIPEFIGGD
                     DESSGFSGGLDEIELLDDISEEPETEKEPEDLSFKKWLGKTGDRQTKTPGKDADHDEK
                     "
     gene            complement(17040..>17747)
                     /locus_tag="PKI68_00615"
     CDS             complement(17040..>17747)
                     /locus_tag="PKI68_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52225.1"
                     /translation="DEPWVALLDGDCIATGDITPYLAPDEPALYARSRSVKEDDRIFR
                     YYRRPGESANGIPERFLKRWREDVGQRTVPARATTVLSGNLILHRDYLDFCRTWKNIM
                     QKVLVHEEPSKTDTAYFMPAEFVLSAWLMFAENPPPVREVQLNSNPEAYLAHLGPAPR
                     YWLFWTQKNLPYFDRIMRLLDLAKEQGYAIPLLPYALKKRNKPAIIAIAFAYRVINSM
                     KWKAKRLYKKFTDSLPK"
     assembly_gap    17748..17847
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17848..18080)
                     /locus_tag="PKI68_00620"
     CDS             complement(<17848..18080)
                     /locus_tag="PKI68_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52226.1"
                     /translation="MQNKPKTVITIIADSNFVIPVYILVLSLKFFQPAQRIHILGISL
                     SGEEKKFFTQFENVFVFDASIPRSNKPGAMRIIA"
     gene            complement(18164..19114)
                     /locus_tag="PKI68_00625"
     CDS             complement(18164..19114)
                     /locus_tag="PKI68_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52227.1"
                     /translation="MKKALIAAASAVFLLVGAVAVDHVFFAAQYTNQIRASHLLRSEL
                     WSIGIVRGEKPFALTVPEKPILRARDITDTAARMVADPFAVQENGQWYLFFEVDSITT
                     HQGEIALATSTDTTNWVYRQIVLDEPFHLSYPYVFKWDGIYYMIPETHQAGAIRLYRA
                     DPFPTHWTHIADLVQGDYQDPTIVRYKDRWWLFACRGQNETMDVFYADVLTGPWHAHA
                     RNPVIAGDRTRARCGGRAREVDGKLIRFAQDCLDRYGHRLLGFEVTTLTPDDYAEQPI
                     KENPLLKPDGSGWNARRMHQLDLYQTGTNSWIGFVDGNAH"
     gene            complement(19127..19951)
                     /locus_tag="PKI68_00630"
     CDS             complement(19127..19951)
                     /locus_tag="PKI68_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX52228.1"
                     /translation="MEYLSFWKMRQPPFESNCDAQFFYESEAHGEALARLLYLVSDRG
                     MGMGAMTGEIGSGKTMVLNVLTSRLRKDLYTTIKLHTAHLPFEHIVAEINQQLRGKGE
                     AAVNDDKYYLLKEFEQLLHNKVSSIGKHLILILDEAQFLSEECLDELKCLTNYNQQES
                     VLTIILSGQPELKAKLSALPQIYQRLGMFCYLKNLRYEEMAPYLEHRLKTAGAEKTDI
                     FNPNSIDPLFSFSGGCPRQINRVCKLAVDRACLMKKEQVDADMIRMIVRDIEKHFG"
     gene            20323..20880
                     /locus_tag="PKI68_00635"
     CDS             20323..20880
                     /locus_tag="PKI68_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52229.1"
                     /translation="MKKLLLILMSTIIAGGAMAGTKPFNLSLTPDVAVYDRNTVIEGL
                     TLSIWGENPQTSFALGFVNGSTGQSAGLSLGLLNYAENYRGLQWALVNYTGQDTSGWQ
                     GGFGFGIAVSALNYTGGTMKGLQTGVVNYAGKLTGLQLGLVNYAKTADAGVQIGLVNI
                     IPQNEWFTRLPDELAPGMILVNWRF"
     gene            complement(21124..22638)
                     /locus_tag="PKI68_00640"
     CDS             complement(21124..22638)
                     /locus_tag="PKI68_00640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNX52230.1"
                     /translation="MSTPSMLVSKLWNYCSILRDDGLSYGDYVEQLTFLLFLKMADEQ
                     SKPPFNKPSAVPAGYGWPSLLAKDGDELETHYRHVLDTLGKKPGMLGIIFRKAQNKIQ
                     DPAKLRRLIVDLIDKEQWSSLSSDVKGDAYEGLLEKNAADVKGGAGQYFTPRALIAAM
                     VDVVAPQPGQSICDPACGTGGFLLAAHDYLAKHHALDRAQKKKLKSGTFFGIELVDSV
                     TRLCAMNLLLHGIGGESEEELPVVTKDALTGKHGEYEIVLANPPFGKKSSVTIINEGG
                     ESSKESLIINRDDFWASTSNKQLNFLQHIFTILKQHGRAAVVLPDNVLFEGGAGETIR
                     RELLKQADVHTLLRLPTGIFYAQGVKANVLFFDRKPAQEKPWTSKLWIYDLRTNLHFT
                     LKENTLKRSDLDDFVNCYFGRDASPQASAAGSASQPYLENRHQRKESERFKAFTYEEL
                     TKRDKVNLDIFWLKDDALEESANLPAPEIIAADITADLEAALEQFAAIAEDLKG"
     gene            complement(22835..24220)
                     /locus_tag="PKI68_00645"
     CDS             complement(22835..24220)
                     /locus_tag="PKI68_00645"
                     /inference="COORDINATES: protein motif:HMM:NF037531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52231.1"
                     /translation="MTEEITIKGTWWLPDNPGNRLSGEITYSPIGGARLSLYGSFSQH
                     SDHEKFTVWGTTVKGTPVSLFDCYTKNVIMHIPGARVAEISSYFGVIGGHFKTPDDLK
                     FFEITAELSHLHEWAWTTGIKVTPTDSGKLWNVEQRMLPDIPLGNRNGLEILLEFAGH
                     LSPGFGDCKLSESCTFTVKSIAPIEYDVFEAVIRKAQHFIALGVSRPVYSLSIKARLD
                     NPGQTPENKTNRKTCEIIRKLSLCDDTDKKLMPHDMQFCLGDLMPAATAYFERFFDKY
                     QLLEPVCDLYFSTLYNPNMYVHQRFLALAHAVEAYHRTFIGGKYQSNDEYQSGLQKIL
                     WDAVPQTLDADFRASLKNKFKYLHEFSLRKRLQDIYGRFVDPLKPFLGEPTKFAGETS
                     ELRNMLTHRDPTDESRPEIPDWTEIWLKCEQLSLLLEVCLLHELGFSEQNISTLLPRN
                     QRAKRIQLNRK"
     gene            complement(24217..25449)
                     /locus_tag="PKI68_00650"
     CDS             complement(24217..25449)
                     /locus_tag="PKI68_00650"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNX52232.1"
                     /translation="MSEPWEIPASWQWSTMGDVAAVVGGGTPKSDQAEYFGGNIPWIT
                     PADLSGYTEKFISAGARSITKAGLDNSGARVMPAGAVLFSSRAPIGYVAIASNPVSTN
                     QGFKSFVLQDGLTSDFVYYYLHHAKKLAVEMASGTTFLEISGKKAAQLPIPVAPLDEQ
                     RRIVAEIEKQFTRLEAGVSALKRVQANLKRYRAAVLKAACEGRLVPTEKWPITTIRDV
                     TLIVTKGSSPNWQGFEYCDDGVVFVRSQNVCWGKLDLSEVAHLPAGFNEKEKKSVLKV
                     GDVLLNIVGASIGRAAIATSDVAGGNVNQAVAVIRCQQEKILPQFLMIYLLSPATQTL
                     IHSQAVDVARANFSLGDISKMPVPIPSLAEQTRIVEEVERRLSVVEELEAVVSANLQR
                     ATRLRQSILQKAFLGELV"
     gene            complement(25446..28235)
                     /locus_tag="PKI68_00655"
     CDS             complement(25446..28235)
                     /locus_tag="PKI68_00655"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HNX52233.1"
                     /translation="MPETPEQKARQQIDAQLVACGWVVQDYKQFNPSAGRGIALREAP
                     LKSGTCDYLLLVDRQAVGVIEAKKEGTLLSGVAEQSGHYAENLPEFINSVTPGMLPFL
                     YESTGVETFFRDERDPEPRSRRLFSFHRPETLAALASEPDTLRRRLAELPVKHPLPAQ
                     NLRACQVEGISNLEESFAAAHPRALVQMATGAGKTYTACSFTYRLIKHAGAKRVLFLV
                     DRANLGRQAMGEFQQFVAPDTGRKFTEVYNVQHLTSSNLDSVARVTICTVQRLYSILR
                     GEELDEDIDEQSGYEIATADDRPKDVAYNPAVPLEAFDFIVVDECHRSIYGLWRQVLE
                     YFDAFLVGLTATPSKQTIGFFNQNLVMEYNHERAVADGVNVGYEVYRIRTEVTEQGGK
                     VEKGFYVDRRSKETRKTRWERLDEDLAYDAKDLDRSVVVKSQIRTILQAYKDALFTEL
                     FPGRTLVPKTLIFCKDDSHAEDTVHLVREVFGKGNDFAKKITYKTYNLETKRYEKSET
                     LIQEFRTSPQLRIAVTVDMIATGTDIKPLECLLFLRDTKSRTYFEQMKGRGTRVLTAT
                     DLQAVSGADALAKTHFVIVDAVGVCESDKTDSRPLERQRTVAFDKLLLGVALGKRDED
                     TLTTLAGRLARLDREISRDEAKKLTELSGGKSLSELSNALLNAVNPDKIAEKAAGPGA
                     EPQEVAPEQLETARQELVAEACAPFDLPALRDALTKAKQEAEQIIDTTTIDRVIVSDF
                     DAAAKEKAENILKSFRDYIEQHQAEIAALQILYSRPFKQRLTEPMLKELEKKLRDNHA
                     AWTEDNLWNAFAVTAPGKVKGRSQAGRFADLVALVRFALEQQPVLAPFADSVTERFNE
                     WLMDKAKSGTAFTSEQLGWLNLIRDHIATAISIEPEDLELSPFNQRGGLGKAHQLFGD
                     QLPKLLDELNEVLAA"
     gene            28427..32035
                     /locus_tag="PKI68_00660"
     CDS             28427..32035
                     /locus_tag="PKI68_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNX52234.1"
                     /translation="MYIATFRRVHAGSGQIADDYVYADGLGRKIQTRAESERNDGSFR
                     VASTVYDEKGRISQAYEPRFEQGGAFVSFVPLNPAVTYQYDAIGRVFKMTPRNGDVGS
                     PTAPTSTTYGYNNNPWVTVVTDALSIKHRFFKNANSQTVLVEEDVEGQTIRTQYVYDR
                     LKRLVTVSDPVANSIHIGYDSLGNKTQMTDPDMGTWNYGYDAAGRMTNQLDAVGNRIA
                     IEYDVHGRPLFKRVYRSGGALEDTVSFAYDSGSTGFQVYDGQLYQITHSGITNRFSYD
                     THGNVLKTELQAAGIGTYTATNSYDALDRPLVAAYPVNSAVVSNRPAAVRYNYNAVGN
                     LDSVASAYGAGSDGAELYKVSSFNELGQIEAVEYGNGVQSRYAYYPGSRRLHQSMTSL
                     ENGGAYQDLSYTFNQGGLITSINDHLRSGKKSATFNDIKYDNLNRLKSLRYSGEVLSK
                     SYSYDQLGNIKKNDDYSLIKTYDYDPNRPHAVTSIGNDEFHYDANGGLTNALDREMTY
                     DARGRLSKVVMTAKGLEVRYSYLDSGERLTKEVVNLSNNRTNLTVYLGKAYEEQRGAD
                     GQETLCHVFFNTKRVASFAPIPAGQQVASVLPDSDALLAKATATASIINPQSKIANPW
                     TRTFARHGTPIRYVTSLADTRLENCIVRLAGLLCTAALLTFLCGREAPVRRFRVKGVV
                     GYGRWKTLDLGPEASGGATFLKTIAQSLKPLDKHHFHLSSFIPHLSAKKVLCTLFSLS
                     FFFQPVYAQGVFQRGDVNGDGLVNMADALLIQQVVDGKRDLKSDWDMAPFAAGFANGD
                     VNYDGATNATDVRIIMEYCVGLRALPAAPVDGSGNEYSQRFTYYHDDHLGSTGIVTDR
                     CGQIVQQVTYTPYGEMRSELNLGASVNYLYTGQQFDREVGLYYYGARYYDQSIGRFIS
                     PDTVIPNPADSQSYNRYAYCLNNPIHFNDPTGHEPVAPNPPPPPPPPPPKPEVKPPEV
                     PPTPAEVNVARIYVELGGTVSVAKPNPANVKQDAKDAAAVSAHVYGDGKTTLPSYISQ
                     LSKEQLENKGLNPSDFNQKNGMVAGLYYNSRTEQYMLAFRGTEGSIKDIATDIRNGLG
                     FKSGQYNSAVMLANDIKKATSGNFMITGHSLGGGLASAASRATGANAITFNAAGLNSA
                     YNKGTSSIQAYITRGEILNVVQAVTPLSNAAGTHITASASHWYSGSFSRHSISNFL"
     gene            32059..32847
                     /locus_tag="PKI68_00665"
     CDS             32059..32847
                     /locus_tag="PKI68_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNX52235.1"
                     /translation="MTRIGWIVLLLGTSTACAEWQPVASRINLFFMKAGDYFEPELQV
                     ELAQAADRGNVSRIKDLVKKGGDVNAQGKDGITPLLWALIKQNEKGLQALLEQGADPN
                     IITTRANGEKFSVLHLAAIMENPDYLRLALEHGGNPNLVDPDSGKTPVYEAIMNRRRE
                     NIDLLIAAGANLNFKDRTGQTPAMIAAGLSQYDVVYEMIKAGADPKITDNWNYTVAWF
                     IEQNPTDPEAESYKWRNLVIDLLRERGMEVLPWTPEKGSGRPSK"
     gene            32955..33953
                     /locus_tag="PKI68_00670"
     CDS             32955..33953
                     /locus_tag="PKI68_00670"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNX52236.1"
                     /translation="MKIEERYLGLLASAFCLLSAGAGAAECQCTSPHASVDCKAPVYG
                     FETADKYQVFKHTFVIENTGSVTLDVTGVKACCGLKATCSTNRIAPQMSAEVTVQFPL
                     SGRTGAQDKMIRVFSSDPHTPEYHLFMRGMVKADDTTRTDIRNVNFGLIRPGATVVTN
                     LIIHGLVNSNRVSVSTPDFSVEQIVTTETTQTVITVKGTAGAQPRLLYGVLQIRGEQT
                     GNSAVEIPLTAAVGWPVMVSPDRITVTPEEAEQPVCRVLFLQSRDQAPVKILKAAVSG
                     NEGQVRIVTIQPDQCRLVVKIAANAGNPPPELRIDAEHNGEQLHISVPVSVRKSGE"
     gene            34634..>35555
                     /locus_tag="PKI68_00675"
     CDS             34634..>35555
                     /locus_tag="PKI68_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52237.1"
                     /translation="MTHNGSGGKNTVRWSGKNAVWLIIGFTFLFFVVGVVLYRTSPNR
                     HQKSDDLALPATSLADKPHQPLSRIVVETNTDISDDRPATLTAELLFSEPGHISGKRA
                     WDCLAELKALGESTREAINELSSSTNVNARLLGVFLTLEVFGPTNSVLEIAVHDPEPV
                     IPAEVGQWLYLNNRFDQWDSFLNNYATNQQPSQMESLLGLCDRVPVSVEVPAGMSILT
                     IGRGLPLLLQEMSRRSDLVAQEISCRILDPSVADNNRLSLFNVMQEARPAGFAAILTN
                     LVSQSTQYSPLRFKAVWSLGETCDDKETLAL"
BASE COUNT         7785 a         9059 c        10193 g         8318 t
ORIGIN      
        1 gtccagattt cgacgtcggg atgatccgcc agcgcgacgc cggccggaat ggtttccggg
       61 tttttaagca gggtttgaat catcgtccgt ttgtcggcgc cggtggcgag aatcagaatt
      121 ttctgcacgc ggtgcagcag gtgcggcgtc acgctgatac ggtcgggttt ttcctttttc
      181 ataaccggga tcgccagacg ttcatcgcgc agtgcagcgt ccttcagatt aaacaacgaa
      241 gcggtgtgtc cgtccgcgcc gaggccaagc agtccgagcg ggattttttt cagtttcctc
      301 aactctgcat caaacgtccg cgcggcaact tcgatcggcc ggtcggtttg gattttcaag
      361 aggttcggaa aaaacgggga gatcgtgccg tagttgcttt gcgggtcggc gacaggaacg
      421 taacgttcgt cgctgagaaa cagaactccc ttggtatcag atactgcgcg gcggtaaatt
      481 tcgagaggag tttttcctcc ggcgagcata acgccgtcgg cagttccaag agttggaaga
      541 atttcatgga acgttgccag acattgggaa atcaactctt ctgaggtttg gaatgttcgc
      601 agtttaatca cgcggaaact ttatgccgta tgtttgcggc ttgtaaagcc ggtgttaaca
      661 attacaagga agcctggaag gctgcggtac gtatcgcagg catcctgcct gccttgggat
      721 gtatctataa gagcagtttg ttgatggcgg cagcggcgcg gcggccttcg cccatggcga
      781 gaataaccgt ggcggcaccg agtacaatat caccgcccgc gaaaacgcgg tcgagcgagg
      841 tttttccggt atcgtcgaca atcagcgtgc cccatttgct ggtctgcaat ccgggcgtcg
      901 tctggcggat gagcggatta gattcgttgc cgattgcgac aatgaccgta tcgacttcca
      961 tttcaaattc gctgccttta atttcgaccg gacggcggcg gcctgaactg tcgggttcgc
     1021 cgagttcata tttcagaact tccatgccgg aaacccagcc gcgttcgtca gcaaggatgc
     1081 gggtaggatt gcgcagcgtg tgaaagatca cgccttcttc tttggcgtgg gcaacttctt
     1141 caacgcgggc gggcatttcg gcttcggtgc ggcggtagat caggtgaact tcttccgcac
     1201 cgaggcggac ggcactgcgg gcggcgtcca tcgcgacatt tcctccgccg agtacggcta
     1261 cttttttcga cgggaagatc ggggtggcgg cgcggttgtc gtagcctttc atcagattgg
     1321 cgcgggtcag gtattcgttt gcggagaaga cgccgaccag attttcgccg tcgatgttca
     1381 tgaatttcgg caggccggcg ccggtgccga tgaagagggc gtcgtagccg tctttttcca
     1441 gcagatcggt cagacggcgg gtgcggccga tgacgaagtc ggttttgatt tcaacgccca
     1501 ttgatttaag gctgctgatt tcctgatcca caatggcttt tggcagacgg aattcgggaa
     1561 tgccgtaaag aagcacgccg ccggggcgat ggaacgcttc gaacacggtt acttcgtggc
     1621 cttcgcggcg gacatcggcg gcaacggtga tgctggccgg accgctgccg atgacgccga
     1681 ctttcttgcc ggtagccggt ttgacgtcgg gggatttgat taatccgttt tcgcgttccc
     1741 agtcggccac gaagcgttcg aggcggccga tggcaacggc tttgtcaatt gacttgagcg
     1801 ctttgccgac cgtgcagggc gcctgacact ggctttcctg cgggcagacc cggccgcaaa
     1861 cggagggcag caggctgctt tcgcggataa taccgatagc tgactgatag tcgccttccg
     1921 tgattttttg aatgaaggcc ggaatgttga taccgaccgg acatccttta acgcagggct
     1981 tggtcgggca ttgcaggcag cgttcggctt ccagttttgc ctgttccccg gaatagccca
     2041 gcgcgacttc ctgcatattg ccgcgtcgcg cgacgggatc ttgagcgggc atttcctgct
     2101 ccggaatggc cagccgctgt ttcggggaga gcggtttggt cagtctggcc agttcctggc
     2161 gggcttcttc agtaagctgt tcgggagtta aatggctcat acaaattatg aactatgaat
     2221 gatgaaagat gaaaaagggt ctgcaacagc ctgactaaaa aggctgacgc cgattaagtt
     2281 ccgatttcat gtttcatatc tcatactaca tccttctctt tgattgcttc ttcgaggtga
     2341 cagcggtggt ggtcggcatc ttcgcgcggt ttgtaggctt tcagacggct gatcatattg
     2401 tcgaagtcca ccagatggcc gtcgaattcc ggaccgtcca cgcagacaaa tttggtttct
     2461 ttgccgacgg ttacacggca tccgccgcac atgccggttc cgtccaccat gatggtgttg
     2521 agcgagacca tcgtatgcac accgtagggg cgggtggttt cagcgcagaa tttcatcatg
     2581 accggcgggc cgatcgcgac agccagagcc ggcttcggat cggcttcgca caattctttg
     2641 agcggtgccg tgacgagcgc tttgcggccg tacgatccgt cgtcggtgca gataatcagt
     2701 tcgtcggcaa tccggcgcat ttcctcttcg agaatcagca gctctttatt acgagcaccc
     2761 atgatgatga tcacttcgtt gccggcggct ttcatcgcct gggcaatcgg gtggagcgga
     2821 gcgacgccaa tcccgccgcc gacgcagatc acccgcccga acttttcaat atgcgtcggc
     2881 tggccgagcg ggccgacgag atgttccacc gtatcaccgg cgtttttcag cgccagcaga
     2941 tgggtggtgc ggccgaccgt ctggaaaata atagtgatgg aaccttcaac cggatcggcg
     3001 tccgcaatcg tcagcggaat gcgttcgccg aaatggctgt tgagctggag aataacgaac
     3061 tgtccggcgc ggcgctcgcg ggcgatcagc ggcgcttcaa tgcgcatctg gaacacttca
     3121 gcggacagct gtcttttaga aagtattcga ttcataagcc gcggattcta ggtcaggacg
     3181 gctgaaatgt gaaacaggaa acctgaaatt ttcggtaata actgcggatc aaagaggaac
     3241 gcagcctgcg gctccaaaca aaaacagcag aagtgctgga aaacggagcg gcagatgcgt
     3301 atggtgcccc gttttcaggc tggatgtcag agccggttcg cggtatcttc tacggctgat
     3361 tgaacagggc aaagggaaaa agacaatgac ggtaacgatg ttgaaatcca agattcacat
     3421 ggcgacgctg accggtacgg aactgtacta cgaaggcagt atttccattg accaggattt
     3481 gctggatgcc gccggaatac tggtcggcga acaggtgcat gtcgtcaatc tgaataacgg
     3541 caaccgttta gtcacctaca cgattcccgg gaagcgcggc tccggcattg ttgagctgaa
     3601 cggcccggcg gcccggcttg gatatgtcgg cgataaagtg gtcattatca cttacgccca
     3661 gatggatgaa gcggaagccc gcacccgcaa gccgaccgtt attctggtcg atgaaaagaa
     3721 cgccattcgc tgatagcgcg atcatatgaa gagggctctg tagccgcgtc cggtggacgc
     3781 ggtttttttg tttagagcaa ataacgattt ctctgacgca atgtcttctt gtaggacggg
     3841 cttcctcggt atcccgagga gcctgtgctt ttgcattacc acacgctccc ggatacggga
     3901 agcgcgtcct acaaccacaa aaaatatgac gtttcaatcg ggaagtgctc tagcgagccc
     3961 acagcgggct cacctacaga atgccactca cgcggcgaaa ctcgcgcccg gtgaggtcct
     4021 cgggcctaca gccattttca gtgttttatt tggttattgc tctaaacaag gctgcgatac
     4081 gtaccgcagg catcctgcct gccttgtcta taaaaccatg tgggtaataa aaaaccccgg
     4141 tagagaccgg ggtttttgag taaccgagca actgttaatc ttcgatttta tccatctgct
     4201 tgcggcggat cagccaggca ccaatgccgc cgatgccgaa tagcaggaac gtagccggtt
     4261 cgggaattgc ctgccagtca ccgcctggtt tcaaaccacc gttaactccg cctgcgtcat
     4321 agttcattgt gccagcagag taaaaaactg ttgtgataga atcatcaatg attgcgcaat
     4381 ttccgctact tagcgagttg tcaaagataa cagaatatat tttgtcgcca gcgttgatgc
     4441 cataacttga gtctgaacca aagatattgt cgatacagaa accattttca cctgcactgt
     4501 tccagttaaa ctgtatgcca gtgtaggtgt cagcgcctag ggatatcgca tttccaataa
     4561 tcgagaaatc aattacgcta tcagtagatt tatacaaccg tatgaagtag tttgtgtcgg
     4621 tatcaagaac cagatccgta gcacccgatg ctttgtatat ttctgtgtta tttccccata
     4681 cgatgtcgga tgctttaacc gatacgcata tcgcgaacat aaacatcatt actaccagta
     4741 tcttattcat gtttttcctt gtttattttt cgtctattca gatcgtgttt ggttaattta
     4801 ttagttcaaa ttatacggtt tagtctcaac ccaagtaaat gcattagttg tctggtacca
     4861 gaatccctga ccgggtttta ttttaacaac cggcggattt gtggctgcga atgatgtatt
     4921 tattaaccac ttgttgttta cattggtgtt agccgacgct ttcaaagcat agtacacata
     4981 ttgttgcgtt gcatcatccc atattcttac ttggtctgcg cgcgtcgcaa gagcactagc
     5041 tattgcaccg tcgttagtgt tgataaggct gtttagatcc atttcaacag gataagggtt
     5101 ggctataaaa gtcaaaggac ttccagaatt tccaacaatg tgatttgtgt acgaatcatc
     5161 gttcggaaca tcgccagaaa tgtagataat tttgttggtt ggagcatacg ttccaggagc
     5221 ggcctgtaac cacatagcct gaccgcgttt cacaatagga tttgtaggcg atccggtaaa
     5281 agctgtatta agataaaaaa ggccatttgt tttttgtcca tatctcacat actgttgaga
     5341 aacttcatcc cacatgatta tcaagtcaca ccgtgtggcc aattggcttt gccgcaactg
     5401 attggttccg aagatatcga gaagggttga aacagttcct gtgcctgaat tgaaaggagc
     5461 cgaaatcagt acatatttgt tcgagacagg atcaaccact tgaatgtatc caaccacatt
     5521 ggcggaagag acggtgttgg tgacggcgga agctgttaca gctataccgg caatcaaaat
     5581 tgcgaccatt tttttcatct gaacaatctc cagttaaata tgattcacat ctgcacatac
     5641 aatatatcgg acgcgactga ttccatccag tttattttag atgaattctc atagttgaaa
     5701 gggacgattt cgccggccgg gggcgtttat tgccgccgtc tgaagcttca ggatgcccgc
     5761 cgaaggggct gtttcggcgc gggccggtga ctgggcatac gatggaaaaa gaaggctccc
     5821 gcgccgtggg cgactgcaaa aaacccgata gatgcgctgt tcggcgaagc cgtagttcaa
     5881 ggctgtctgg ctatgattca ccctcatttt ttccgtgtaa tccgcgcaat ccgtggttaa
     5941 atgtcccgcc ttttaaccga tgggagagct tttgtgaata aaactgactt tttgaggaaa
     6001 ctgcctggat ccgatgtctg ttactacagc ctgccggagc tggaaaaaca gggcggctat
     6061 gatttttccc gtatgccgtt ttctgtgaag gtactgctgg agtcgctgct ccggatgcag
     6121 ggccatccgg cctatacgcc gcaacatgtt acgcagctgg cgcaatggtc gccgcgcagt
     6181 gaactggtcg agctgccctt tatgccggcc cgcgtgctcc tgcaggactt taccggcgtg
     6241 ccgtgcgtgg tcgatctggc ggcgctgcgc tcggcgatgc agaaagccgg caaagatgcg
     6301 tcgcggattg agccgcaggt gccggtggat ctggtggtgg atcattccgt gcagctcgac
     6361 gcctccggtt gtgctgcggc gctggagctc aacgtggcgc gcgagtttga gcgcaacggc
     6421 gagcgctata agtttctgaa atgggggcag ggtgcttttg aaaaactgcg cgtacttcct
     6481 cccggtctgg gcatctgcca tcagattaat atggaatatc tggcggcctg cgtagcgttt
     6541 gaggacggaa tcgcctatcc cgacacgctc gtcggaacgg acagccacac gacgacgatc
     6601 aactgcatgg gcgtgctggg ctggggcgtc ggagggatcg aagccgaggc ggcgatgctc
     6661 ggccagccga ttccgattct gacgccggaa gtggtcggct ttaaattcac cggcgcgctc
     6721 aagcccggcg ttaccgccac ggatctggcg ctgacggtca cccgtctgct gcgcaaaaaa
     6781 ggggtggtcg ggaaattcgt cgagttctac ggcgaaggcg cggccaatct gagtctggcc
     6841 gaccgctgtc cggtcgccaa catggcgccg gaatacggcg cgacgatggg cttttttgcc
     6901 gtcgatgaaa aaaccatcgg ctatctgcgc gaaaccggcc gcagcgaaga gcagtgcgcg
     6961 ctggttgaag cctactgcaa ggctcaggga atctggggct ttgagagcga ggctgactat
     7021 aacgacacgc tggaactcga tctttccacg gtcgaaccgg cgctggccgg accgagccgt
     7081 ccgcatcagc agctttcgct cgccggtctt aaagaggaat tccaaaagga cttcggcggg
     7141 cagggtgccg ggagcacgga gcagggggga gaagaannnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnatca ccagctgcac gaatacgtcc aatccgggcc tgatgatggg
     7321 cgcggcgctg gttgcgaaaa aggccgtcga aaaggggctg acggttccgg cgtttgtgaa
     7381 gacctcgctg gcgcccggct cgcaggccgc cacgcgctat ctcgaaaaag ccggactgct
     7441 cgaaccgctg aacaggctcg gattccaggt cgccgcctac ggctgcgcca cctgtatcgg
     7501 caacagcgga ccgctggcgc cggagattga ggaagagatt aaggccaagg atctgacggt
     7561 tgccgcggtt ctttccggta accgcaactt tgaaggccgc gtgcatccgc tgacgaaagc
     7621 caactatctg gcttcgcccg cgctggtcgt cgcctatgcg ctggccggta cggtcaacat
     7681 cgatctcgaa accgaaccgg tcggcaaaaa tgcgtccggc gaagcggtat tccttaaaga
     7741 catctggccg acgacggacg agctgcaggc ggccatcgcg ctggccgccg acccggcgtt
     7801 ttacacggag gtgtacggcg acgcgttcaa catcagcccc gaatggaacg cgctggaaac
     7861 cgtgaagagt caggtgtttg aatgggacgg cgattccacc tatatccgcg agcccacgtt
     7921 cttcgaaggc tgcgggcagc cgcccgcgcc gctggcggat atccaaaacg cctcggtgct
     7981 ggggctgttc ggcgacttca tcacgaccga ccatatttca ccggccggca acatttcgaa
     8041 gaagagcccg gcggcggact atctgcgggc gcacggcgtt gccgaacgcg atttcaactc
     8101 ctacggttcg cgccgcggca accacgaggt gatgatgcgc ggaaccttcg ccaacatccg
     8161 catcaaaaat aaaatggtcg aaggcgaggg cggacgcacg gtttatctgc cgaccggcga
     8221 cgagatgttc atttatgatg cggcgatgaa atatgccgaa tccgccacgc cgctggtgat
     8281 tctggcgggc aaaatgtacg gggccggttc gtcgcgcgac tgggcggcca aaggcacgaa
     8341 actgctcggc gtcaaagcgg tcatcgcgga aagctttgag cggattcacc gctcgaacct
     8401 ggtcgggatg ggcattctgc cgtgtgaatt catcaatggc gaaacggcgg acagtctcgg
     8461 gctgaccggc cgggagagct tcacgatcac cggtgtggcc gatcatttcg tgcccggcaa
     8521 gattctgacg gttcgtgccg gcggcaaaga gtttcaggtg aaagcgcgcg tcgatgcgcc
     8581 gctggaaatt gaatacctga aaaacggcgg cgtactgaat tatgtgctgc ggaatctgat
     8641 ggcgtaacac ccgccgatcc acaaggggtg atgaggattc atcacccctt gttggatcaa
     8701 aaccattcct gagcgatctt ccagccgatg aagaggccgg cgatgctgcc caggccgctg
     8761 acgaaaaatt cgccccacag gccgaacccc atgattccgc cgagccacca gccgatccag
     8821 ccgccgaggg tgatgccgac ccagatgagg agttttgtca tttcagaacc tcttcataga
     8881 ttttcatgac gcgttccgcg atggcgctcc agtcgtagtt ggctgaaact tcggcgaatc
     8941 cgttcgttgc gagtttggcg cgaagttccg gcgagccggc gagccggttc atttgttctg
     9001 tcagccgggc gtcgttgttg tcgtccgtga gcagaatatt ttcgccgtcg gtggtgaagc
     9061 cggggatgcc gccgacgcgc gtggcgatca ccggcgtacc ggcggcccac gcttcgagga
     9121 tgacgatacc gaacggttcg ttggcgctcg gcagaacaaa caggtccgcc gctttataag
     9181 cagacggcag cagcgggtcg tcgggtttga gcccggggat tatcagcacc gcgtcgttca
     9241 gccccagccg tgcaatttcg gcgattatct ggcggtggta ttcttcgacc gaaaccgagc
     9301 cgataaagac gaggttccag tcgggatgcg ttttgcggaa ttcggcgaag gctctcacca
     9361 gcagcagctg gtttttctgg aagtcgatgc gcgacaggca gagtacgaat tttctgtttt
     9421 ccagtccata ggcgttgcgg aaggcgtccg gcccggcggc cgcaaagcgc cggacgtgaa
     9481 cgccgttggg caggtagtga atgttgcggc ccggcgagcg ccggcgcatt tcatccgctt
     9541 cgctctggcc gacgcagatg acggcggcgg catcgtcaaa cacccgccgc gagccgaaga
     9601 gcaggccgaa gagtttgccc cactcccgct tgccttcgac cggcgcgacc atctggtcgt
     9661 actgatcctg cggaacggtg tggcgtccgc cgtggacgtg gatgacgtag ggcacccggc
     9721 gcaggcgggc gacggtgcgg gcgatgccgc cgagccgccg gccgacgtgc gtatgaatca
     9781 gcgtcaggtc tttctggaag agcagggcga agaagagcgg cagcgagagc gggctgccgc
     9841 ctttgagccg cagtgcgctt ttggcttctt cactcaggcc gaaccacggg aagcagtagc
     9901 ggaagcgccg gacggtgacg ccgtcgattt tttccgcgcc ggttcgggca aacatgtcgg
     9961 tgcaaaaaat cggactgttg agtccgttct gttcgagttc gcgggcgagg ttgaagacca
    10021 cggtttcggt gccgccccat ttgtcgggaa caaaccggcg catgacgtgg gcaatggtgc
    10081 cgtgaagttt tctcatgcgc ggaatctact caaaacattc gtccgacgaa agagttttcc
    10141 ctggggtcgg caagctttgg tcttgcaact tctcgccatt ccgccgttcc attagcgcat
    10201 ggatttcgac tggctcatta tagacggcta caacctgatg catcaggacg gcgcgatgga
    10261 aggatcgcgc agcgacctgc cgaccgcccg tcagcggctg gtgcgccgta ttgagcgaac
    10321 ggcgactgaa ctggcttcgc gtattaccgt ggtgttcgac ggacgcgagg gcgggcgcga
    10381 tgctgttttt gacgcgccgc atctggaggt gatcttttcg ccttccaacc gcacggcgga
    10441 cgggttgatc gaacagatgg ttcatgatgc aaaacagccg gagcggattc tggttattac
    10501 ctccgactgg acggagcaga ggctggtctc tgtttttggt gcgtcggtga tttcctgccg
    10561 cgagtttctg ctgcgctgtg agccggcgcg gacgccgccg aagaggttga agtctgcgcc
    10621 gggttcaaaa cttggagatt tttttccgga ataaaaatat gaaaaataca aaaaaacagt
    10681 cccgttctcc ggtggttgct ttagtcagtc tcggttgcgc caagaatacc gtggattccg
    10741 aactcattct cggacgcttc gttgagagcg gctggatgat tgccgaggag ccggcggacg
    10801 ccgatatctg tctcgtcaat acctgcggct ttattcatga cgcgcgcgaa gaggccgccg
    10861 gcgtgctgca cgaactgcgc ggactcaaaa acggcggcgg cccgcgcgcc gtcgtcgcgg
    10921 tcggctgcct cgtgaagcgt gtcgccgaca gtccggagct ggagaaattt ctggaagccg
    10981 ccgacgcccg ggtttcgttt cctgattaca gccgtctgcc ggaaatctgc gccggactgc
    11041 tgggtgaaaa aacggaagcg tccgagccgg aagggtacag tgacttcctg atccgtccgc
    11101 gcctgcggac gggttctccg cacaccgcct atctgaaaat ttctgaaggc tgttccaatc
    11161 cctgcacctt ctgctcaatt ccgaagattc gcggaaagca ggtcagccgc ccgatggatg
    11221 atattgttga tgaagcgcgg gcgctgatgg aagcgggcgc ggttgagatc aacctgattg
    11281 ctcaggatac gaccgcgtac ggcaaagact gggacggcga actgcacctc aagacgctgc
    11341 tgtgcagact gcgcgacgaa atcgcggaag atatctggtt ccggctgatg tatgcctgcc
    11401 cgcagcactt gacgcgcgac gtgctcgaaa cgatggcctc cgacccgcgg ttctgtccct
    11461 acatcgacat gccgcttcag cacatttccg accggatgct cgacgcgatg ggccgccggg
    11521 ttactaaagc ccagacgctg gagaagctcg atctgatccg cgcggtgctg ccggactgcg
    11581 ccatccgcac ggcgttcatc accggacatc ccggcgagac cgaggcggat tttgccgagt
    11641 tgctggcgtt tataaaagaa ggccgcttca cccatgccgg cgttttcacc tattccgccg
    11701 agccgggaac actttccgcc aaactggaag acggcgtacc gccggcggtt aaagaacagc
    11761 gccgaacact gctgatggaa gcgcagcgcg aagtctccgc cgcgcgctgc gcggcgcagg
    11821 tcggcagaac ggtcgaggtg atgattgacg gtcacggcga agaaggactg gtcagccgct
    11881 cacagcgcga agcgccggaa gtggacggcg tggtttttct gccggagttt gacgcggttc
    11941 cgggagaccg gtttgatgtg cagattaccg ggtcgctcga ctacgacctg attgcggaat
    12001 aacggcggat tttattgcgg acggttgttg ccgtagttga acggatcaac tttccgctgc
    12061 cagatatcgg ttgccgcgtc gcgagtgatc gtggttccga acatggtgac ggcctgtttg
    12121 acaacatccc ggcagaatcc gaattttcgc ggcacggcgt agacaatgtc tccggcctgg
    12181 agggtgacgt ccagcgcatg gccgctgagg attttgaaga cgcgcgcttt cttggagtcg
    12241 aactgcgaca tatccatgag catgactttt ggattggtcg tgttgccgcg gatgataaga
    12301 acatggccgt cgccgagttc acggttaatg ccgccggcct gaacgacggc attcatgagg
    12361 gttgtctgcg aagaggcaaa ctgaatgccc tgcggattat tgaaagctcc cagggcgtag
    12421 attttgcggt ccagtgctga aggaatagtg atgatgtcgc cgctgcggat ctcgatattc
    12481 tgtgaagcgt cggctttata aacgagacgc tcaaagtcga ccggaatgac ttctccgccg
    12541 cgcaccagtg tggcgtgagc gtagtcgtga atgtcggcgc tgacatcgcg gatgacgccg
    12601 actttcggac cgccggcctg agcaagggca tccagaatgt aggtgcggcc ctgcagcgga
    12661 tagacctgcg gcttgttgag ctgtccgagc acggtgaagg tctggctgct gaagcggacc
    12721 ggaataacgc tgaggatcga atagttcagt ttttcgttca ggcgtttgcg catgtcggcg
    12781 gtgaggtcgt cgatggtccg gccggctgcg ggaattgtgc cgagaagcat ataggtgatg
    12841 tttccggacg ggtcgatcgg cagtgtgcgc cgcgtttcag catcaccttc accgtaaaga
    12901 gtgatttgaa tgacatcacc cacgccaagc agataagggc cttctttcgg tttggtgagc
    12961 agtttggtgt caacctgttc atccgggttg agctgaatcc atacgcccgt ttccatttct
    13021 ttcagcgtca gatcgccggc attgggcagt tcgagtactt cggcgggctg aagttccttg
    13081 ttgctgttca gccgcggctt gtcgcgcaac gttgtgcagc ctgcggtgat gattgccgtc
    13141 agcagaatcg ggagaagaat gaactgtttg attttcatgg ggtggtcacc ctgggtgtag
    13201 tgaacccgcc ggggaacagg tatttgcggt taaggtcgct ggcgaattcg ctggcaaagg
    13261 tgttgacgaa ggtgctgacc atcgctttga cgagggtgcg ggtgaacagg aatgattttt
    13321 caggaacgaa aacaatgtcg cccgcttcga ggaacacgtt gtgttctttg ccgcgcataa
    13381 tggcgtccag attgactttg aatactttcg gatcgttcat tgcgtcgcgc agaatgacaa
    13441 cccgtttaat gtctgccgtg tcctgcttat agccgccggc gccggcatca agtccggaga
    13501 tgagctgggt gagcgtcagg ccggcggtaa acggctgcga ctggggctgg ttgacttcgc
    13561 ccatgagata aatttcacgg cccagcgcgg aggcaatgta aaggacatcg ccgggacgga
    13621 catagatgtc ttcggagaaa tcgtctttat ccatcagggc ggcgaagttg actggaattt
    13681 tttcctgtcc gcggagcagc cagctgtctt tatagttatg aatcggaatc cagctgtcgt
    13741 gatacttacc cagagcgatg ccctgtgctt tcgcaatgga ctgcctgaca gtggtcggtg
    13801 tgtcgagcgg ataaatgccg ctcgactcga cctttccgaa gatggcgaag cggttggaga
    13861 tgaagcgtgt gggtatgatg gatacttcgg gattgttgaa gagccggccg agtttggact
    13921 ccatttcttt cgccacttcc tgggcggtgc ggccttcggc cggaacgccg ggcaggaaca
    13981 tatagtagag tttaccgtcc ggcgcgaccg gtacattttc ggcaatcgtg tcgcgctgtc
    14041 cgaaaacgcc gatttcgact tcatcgccga cctgcagttt acggtcttcc ggactgtgcg
    14101 ggacgaaata ttccggcgca aacttcgagc gtttttgtgt cgccgtaatc gggctgtccg
    14161 tgctgacagg agcggcagac tcctcggcgg cccgggtcag acccgccaga agcagcagga
    14221 tggcagtaat gacgggctta tttctcatcg tggtcagcgt ccttaccggg agtttttgtc
    14281 tgccggtcgc ctgttttacc gagccatttt ttgaaagaga ggtcttccgg ttctttttcg
    14341 gtttccggct cttcgcttat gtcgtccagc agttcgattt catccagtcc cccggagaac
    14401 ccggatgatt cgtcgtctcc gccgatgaat tcaggaatgt cttcatcctc cgggaacccg
    14461 gccgtttcat cttcgaacgg ttgaccggaa acctgttctt tctgcgctgt tccggcagcc
    14521 ggttcagccg gttttctgaa gcgggcgaag aggccgagca gcggctggag gcggtcaaga
    14581 atttctgcca gcggattgcg cggttccgcc agattgcgga tgatgcgctg ctgccggacg
    14641 tcttcaagat agtacgggtt gacgcggttg aaaatgatgg cgcgcggtgc aaggccgcgg
    14701 tcttccagaa attcaaggcc ggcatcgaca agacggcggt cactgaccgg cgaggccagc
    14761 acataaatga tctcatcctg aaaaccggcg atggtgccgg agatcgggtg gtcaagaacg
    14821 gccggtgact cggtgatgat caggtcgtag ttctgccgga gcagatccca caggcgcggc
    14881 atggatgagc tttttgccag atcaagcaga tcggcgcgca gttcctgcac acggattaca
    14941 tcaacgccgt tgatattggt aaccatgtcg gccaattcag cggtgccgcg cagatagtcc
    15001 tcaattccca tttgcggcca gccggtttct gctgcgccgg gcagacaggg gttgggctgt
    15061 gcgtcgaagc cgacaaagag caccttgatg tgcagggagc tgtagtagcg cgcaaggttg
    15121 aaggagagga tgcttttgcc ttcaccgtcg agcgagctga agaagccgat gacttttgcg
    15181 cgctggccct gcagaatcac attcagccgt tctgaaattt cacgcagaga cggcaggaga
    15241 agctggtagg tgtcgtaatt ctccaggttt ggaacttcaa tgatcgaggc caggcagggg
    15301 gcgctgtaag cattttcgag ctgctgctgt gtgcggattt tcgcgtcgag cagttcgatg
    15361 atcagcacca gcaaaaggcc gccgaaaaag ccgaagatga atccggcaat cggaatgatt
    15421 tttccgatca gtgatgcggc tgtaggttcg gcagttgttg cagggctgat gagcttaaag
    15481 tcagacaggc tggaattcat ggtcagacgg gcggattctt cgctggcttt cagagatttc
    15541 agcgaggcat ccgtctggac gcgtttttcc agcagggcgg cgtattcttt ttcaaggcga
    15601 ggcagatcct ggaatttcag ctggagatca tccaggtctt tttgcagagc ggttacctgt
    15661 tgcgccgcga cattttcttc ggagcgcagc ttgaggagtt caccttcaag ctgtcccttg
    15721 agggggtttt ttacatacgt gcgttcacgg gtttcatcat agcttccgct ctggagcagt
    15781 ttgcgcagct ctgcgatttc gcgttcctgc cgcagaattt tcgggttgtc ggcggcgtac
    15841 tgaatgcggg catccagcag ggcggcttcg gcattggaga tgcggcgttt gagcggattt
    15901 tcttccagtg attcgttcag aacttcttcc ggaagttcgg agatcatttt ttcgtagttt
    15961 ttgatgcgga caagaagtcc gtcatgagcg acctgcgcaa tgctgagccg ttcgctggcg
    16021 gcattgacgg acgaaaagta gttctgatac tgcgtgttga gttccagaat cttgtttttc
    16081 agctggaatg cctcaacgga ctgtacggcc tcgttgaagt tcttttcagc aacttcgcgc
    16141 tgggtcttat acagttcgta cgccgaagcg gccagctgtc tgtaatactg gtcgttgtcg
    16201 gaaatgatga tgtcggccag cttgttgacc gcggcaactg ccgttttttc atccggtatg
    16261 ccggtgactt tcaatgtgac gatatcggat ttgctttgcg aatcgaacga gacgtttttc
    16321 cggagcggtt ttaatccgac ggcatcttcc agagtcttga tgtgcgcggg cgaaagaaac
    16381 atgtcaacga gtgtatcctg cgagagcgga cgcatcagaa acgtcgaacc atgcgtgtcg
    16441 atgtgtttct gcgtgggctt ggtgtagatg aataccgatt gagcagacca tgtgacgccg
    16501 ccctgtaaaa cgcgtgctac aggaatcagc aggatcatca tgccgaacgc ggacagcaga
    16561 acgatccaga acttgcggta aataccgcga acaattttga tcagatccag attgcggaag
    16621 aaatcctgaa aggcgttttc cttgcggatg gtacggccgg gccgtgcggt ttccgccatg
    16681 ccggcccccg gaatggatgc tttggacggg cggacggtgt cgcgcgacaa tgtcggaggg
    16741 atgtcgctga gatcgctgaa ctgatctttg atgcggaacg gaggttcttc gccgaaccgg
    16801 ggtgccggct ctttttcttg atccggtcgt tttccggcag actcttcagc ggacgttccg
    16861 gcggttccgg atgtttcttc cggagtttta tccgcaggtt tattttccgt aactggcgga
    16921 acggccggtt tttcttcagc gggttttccg aagtgaaggt cttcgctcgg tttatctctc
    16981 atagttcatc ccagctgtct ttggatagtc acttagaccc gcggacgtct ccgcgccggt
    17041 catttcggca gactgtcagt aaattttttg taaagccgtt tggccttcca cttcatcgaa
    17101 ttgatcacac ggtatgcaaa agctatcgct ataatggccg gcttattacg ctttttaagc
    17161 gcgtaaggca ataaagggat tgcatatccc tgttcttttg ccagatccag cagacgcatg
    17221 atccggtcaa agtagggaag atttttctga gtccagaaca gccagtatcg cggtgccggt
    17281 cccaggtggg caagataggc ttccggattg ctgttgagct gtacttcgcg aaccggcggc
    17341 gggttttccg cgaacatcag ccaggcgctc aagacgaatt ctgccggcat gaaataagcg
    17401 gtatccgttt tggacggctc ttcatgcacc agaactttct gcatgatgtt tttccaggtt
    17461 ctgcagaagt cgaggtagtc ccgatgcagg atcagattgc cggaaagcac cgtggttgcg
    17521 cgggcaggga cggtgcgctg gccgacatct tccctccagc gctttagaaa ccgttccgga
    17581 atgccgtttg ccgattcacc gggacggcgg tagtagcgga aaatgcggtc gtcttccttg
    17641 acggaacgcg accgggcgta gagcgcaggc tcatccggcg ccagataagg cgttatatcg
    17701 ccggttgcaa tgcaatctcc gtcaagcaga gcgacccacg gctcatcnnn nnnnnnnnnn
    17761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17821 nnnnnnnnnn nnnnnnnnnn nnnnnnngcg atgattctca tggcgcccgg cttgttgctg
    17881 cgcggaatgg aggcgtcaaa aacaaacaca ttctcaaact gggtgaagaa ctttttttcc
    17941 tctccggaaa gtgagatgcc gagaatatga atacgctgtg cgggttgaaa aaatttgagt
    18001 gacagaacca gtatgtaaac cgggatgaca aaattgctgt cggcaatgat ggtaatgacg
    18061 gttttaggtt tgttttgcat agccggatga atggaaccta ttttgggcgt atattgcgtt
    18121 ttgcggtatg cggcgtccag taaatagctt acgtaagcac gttttaatgc gcattgccgt
    18181 caacaaaacc gatccagctg tttgtgcccg tctgatacag gtccagctgg tgcatccggc
    18241 gggcgttcca tcctgaaccg tccggtttga gaagcgggtt ttctttgatg ggctgttcgg
    18301 cgtaatcgtc cggagtaagc gttgtcactt caaatccgag cagccggtgt ccgtaacggt
    18361 ccaggcagtc ctgtgcgaag cggatcagtt tcccgtctac ctcgcgggcc cgtccgccgc
    18421 agcgtgcgcg ggtgcggtcg ccggcaatca ccggattccg ggcgtgtgcg tgccacgggc
    18481 cggtcagcac atcggcataa aaaacatcca tggtttcatt ttgcccccgg caggcaaaca
    18541 gccaccagcg gtctttgtag cggacaatgg tcggatcctg ataatcaccc tgaaccagat
    18601 cggcgatatg cgtccagtgc gtcggaaacg gatcggcgcg gtagaggcgg atggcgccgg
    18661 cctgatgggt ttccggaatc atgtaataga tgccgtccca tttgaaaaca taggggtagg
    18721 aaagatgaaa cggctcatcc agtacaatct gccggtatac ccagtttgtc gtatctgtgc
    18781 tggttgccag ggcaatttca ccctgatggg tggtgatgct gtcgacttca aaaaaaagat
    18841 accactgtcc gttttcctgc accgcaaacg gatcggccac catgcgtgca gcggtatcgg
    18901 tgatgtcgcg ggcgcgcaga atcggctttt caggtaccgt cagtgcaaac ggtttttcac
    18961 cgcggacaat gccgatagac caaagttcgc tgcgcagcag atggctcgcg cggatttgat
    19021 tggtgtactg cgcggcaaag aaaacatgat caacagcaac ggctccgacg agaagaaaaa
    19081 cagcggaggc agcggcaatg agcgcttttt tcatcggtaa ctccggtcag ccgaagtgct
    19141 tttcgatgtc gcgcacgatc atgcggatca tatccgcatc aacctgttct tttttcatca
    19201 ggcaggcgcg gtcgacggcc agtttacaga cgcgattgat ctgacggggg catccgccgg
    19261 aaaatgaaaa gagcggatca atactgttcg ggttgaaaat gtcggttttt tcagcgccgg
    19321 ctgttttcaa ccggtgttcc agataggggg ccatttcctc gtagcgcagg tttttcaaat
    19381 agcagaacat gccgagccgc tggtaaatct gcggcagggc gctcaatttg gctttcagct
    19441 caggctggcc ggaaagaata atggtcagca ccgattcctg ctggttgtag ttggtcagac
    19501 atttcagctc gtcgaggcat tcttcgctca ggaactgcgc ttcgtccaga atcaggatca
    19561 ggtgtttgcc gatggagctg actttgttgt gcagaagctg ttcgaattct ttgagcaggt
    19621 aatatttgtc gtcattgact gcggcttcgc ctttaccgcg cagctgctgg ttgatctccg
    19681 cgacgatgtg ctcgaaaggc aggtgggcgg tgtgcagttt aatggttgta taaaggtctt
    19741 tgcgcaaacg ggatgtcagc acattcagca ccatggtttt gccgctgccg atttctccgg
    19801 tcatggcgcc catgcccatc ccgcgatcgg aaaccagata gagcaggcgt gccaaggctt
    19861 cgccgtgtgc ttcgctttcg tagaaaaact gcgcatcgca gttcgattcg aatggcggct
    19921 gtctcatttt ccagaaggat agatattcca tagctcgtaa cctgcttttg ccgggagaga
    19981 atagatcaag tcactcacgg tttgaaagaa aaacctgcaa ccaattgatg ttcaatggat
    20041 ttacacgccg gggcaggtcg ccgtaactga cgccagggtg cggtaatctg gatatctgac
    20101 cggctgctaa aaaaaagacc tgaacgaaaa ttgccggaag tacagcgcgc gccggtgcct
    20161 cgatgaaaag accggtgctg agatacacga acagatcaaa agagggtata ctccacagcc
    20221 agaaagttct gaaacaaaac gaaaggaaat tctatatggt ttttattcac accatcggta
    20281 acataggcgt caggtaaacc ggcagaagag gagaaacgaa cgatgaagaa attactgtta
    20341 attttaatga gcacgataat cgccggcgga gcaatggccg gaaccaaacc gttcaacctc
    20401 agcctcactc cggatgttgc cgtctacgac cggaacacgg tgatcgaagg gttgaccctc
    20461 agcatctggg gcgaaaatcc gcagacgtca ttcgcgctag ggtttgtcaa cgggtccacc
    20521 ggacaaagcg ccggactgag tctgggactg ttgaactacg cggaaaacta tcggggactg
    20581 caatgggcgc tggtcaatta tacgggacag gatacttccg gctggcaggg cggtttcggt
    20641 ttcggtattg ccgtcagcgc gttgaactat accggcggca ccatgaaagg gttgcagacc
    20701 ggcgtggtca actatgccgg caaactgacc gggctccagc tgggactcgt caactacgcg
    20761 aaaacagcgg atgccggagt gcagatcggg ctggtaaaca ttattcctca gaacgaatgg
    20821 tttacccgtc tgccggatga gctggcgccc ggaatgatcc tcgtcaactg gcgcttctaa
    20881 gacgaaaaaa ggccggctgt ttgttcagtc cggcccgccg tgagcgaggg tcaattcgcg
    20941 tggagccaat gatcgggatc gaaccgattg tgctgccggc gcgaagagat acccaatccg
    21001 ggcagtgaat gatcgcaggc ataaagatgg aaacgacatg gatattcatc gcgtttcgac
    21061 tgtggcgtgc ggggaaatgc tacgcaaata ccgccggacg catccggctc cggaaaaatt
    21121 accttatcct ttgaggtctt cggcgatggc ggcgaactgt tcgagagccg cttcgaggtc
    21181 agcggtgatg tcggcggcga taatttccgg ggcgggcaga tttgccgatt cttcgagcgc
    21241 gtcgtctttc agccagaaaa tgtcgaggtt caccttgtcg cgtttggtca gctcttcata
    21301 ggtgaaggct ttgaaccgtt cgctttcctt gcgctggtgg cggttttcaa ggtagggctg
    21361 gctcgccgag ccagccgcgg acgcctgcgg cgaggcgtcc ctaccaaaat aacaattcac
    21421 gaaatcgtcg aggtcgcttc gcttgagggt gttttccttg agcgtgaagt gcaggttggt
    21481 gcgcagatcg taaatccaga gtttggatgt ccacggcttc tcctgtgccg gtttgcggtc
    21541 gaagaagagg acgtttgctt tcaccccttg cgcatagaaa atgccggtcg gcaggcgcag
    21601 cagcgtatgc acatcggcct gcttgagcag ttcgcgccgg atcgtttcgc ccgcgccgcc
    21661 ttcaaagagc acgttatcgg gcagtaccac cgccgcacgc ccatgctgtt tcaggatcgt
    21721 gaaaatgtgc tgaaggaagt tgagctgttt attgctggtc gaggcccaga aatcgtcgcg
    21781 gttgataatc agcgactcct tggagctttc gccaccctcg ttgataatcg tcacgctgct
    21841 cttcttgccg aacggcggat tggccagcac gatctcatat tcgccgtgct tgccggtcag
    21901 cgcatccttg gtcaccaccg gcagttcctc ttcggattcc cctccgatcc cgtgcagcag
    21961 cagattcatc gcacaaaggc gcgtcacgct gtccaccagc tcaatcccga agaatgtgcc
    22021 gctcttgagc tttttcttct gagcccggtc gagtgcatgg tgcttggcca gatagtcatg
    22081 cgccgccagc agaaagccgc ctgttccgca ggccgggtcg caaatggact gtcccggttg
    22141 aggagcgacc acgtccacca tggcggcaat cagcgctcgc ggcgtaaagt attgccccgc
    22201 gccgcccttc acgtccgccg cattcttttc cagcagccct tcgtaggcat cgccctttac
    22261 atcggacgac aggctcgacc actgctcctt gtcgatcaaa tccacgatca gccgccgcag
    22321 cttggccggg tcttggattt tgttctgcgc cttgcggaaa ataatgccca gcatccccgg
    22381 ctttttgccc agcgtatcca gcacatggcg gtaatgggtt tccagttcat caccatcctt
    22441 ggccagcaga ctcggccatc cgtacccagc gggaacggcg gagggtttgt tgaacggcgg
    22501 cttcgactgc tcgtctgcca ttttcaggaa aaggagaaac gtcaactgct ccacataatc
    22561 gccatacgac aggccgtcat cgcgcagaat cgaacaataa ttccacagtt tagataccag
    22621 catggagggc gtgctcatat aacacctagt ttgttttgaa attcaatggc gcagatcgtg
    22681 gttggagttt tgcagcctgt ccgccgtagc tcaacgagcg aaggaggata attccacccg
    22741 agacaaaggc gagagaggct ggcgcgaatg cgacggcaac ggaacgaagt gcagtggcaa
    22801 agtttggaaa cgagttgttg cgggtttgtc atgattattt ccgattaagt tgaattcttt
    22861 ttgctcgttg atttcgtggc aacagggtcg agatattttg ttcgctgaat ccaagctcat
    22921 gaagcaggca cacttctaga agcagactta actgttcaca tttgagccaa atctcagtcc
    22981 agtcaggaat ctcgggacga ctctcatcgg taggatcgcg gtgcgtcagc atgtttctca
    23041 attcggaggt ctctccggca aattttgtag gctctcctaa aaagggtttg aggggatcaa
    23101 caaagcgtcc ataaatatcc tgcaagcgtt tacgaagcga aaattcatgc aggtatttaa
    23161 atttgttttt caagctagct cggaaatcag catcaagggt ttgaggtact gcatcccata
    23221 agattttttg aagtccactt tgatactcat cgttagactg atattttcca ccgataaaag
    23281 tccggtgata tgcctcaaca gcatgcgcca atgcgagaaa ccgttggtga acatacatgt
    23341 tcgggttgta aagcgtcgaa aaatacaaat cacagactgg ttctaaaagc tggtatttat
    23401 caaagaagcg ctcgaagtag gctgttgcgg caggcattaa gtcccctaag caaaactgca
    23461 tgtcatgggg cataagcttt ttgtcagtat catcacacaa ggagagtttc cggatgatct
    23521 cgcaggtttt ccggttcgtt ttgttttcag gtgtttgccc ggggttatct agacgggctt
    23581 tgattgaaag agaataaaca ggccgggata cacccagggc aatgaaatgt tgagcttttc
    23641 gaatcactgc ttcaaagacg tcgtactcta ttggagcgat ggatttcaca gtaaaagtac
    23701 aggattccga caacttacaa tctccaaagc ccggcgaaag atgcccagca aactccagta
    23761 atatctccag accatttcta tttccaaggg gaatatcggg aagcattctc tgttcgacgt
    23821 tccacagttt tcctgaatcg gtcggcgtca ctttgattcc ggttgtccag gcccattcgt
    23881 gaagatgact cagctcggca gtgatttcga aaaacttcag gtcatcgggt gttttgaagt
    23941 gaccgccaat tacgccaaaa taggatgaaa tttcggcgac gcgcgcaccc ggaatatgca
    24001 taatcacatt ttttgtgtaa caatcaaaca agctgaccgg cgtgcctttt acagtcgttc
    24061 cccaaacagt aaatttttcg tggtctgagt gttgggagaa actgccgtat aagctcaggc
    24121 gagctcctcc gattgggctg taggtaattt ctccagaaag ccggtttcct ggattgtccg
    24181 gcaaccacca tgtgcctttg attgtgattt cctcggtcat acaagctctc ccaaaaacgc
    24241 cttctgcaaa atggactggc ggaggcgggt ggcacgctgg aggttggcgg agaccactgc
    24301 ttccagttct tcgaccacgc tcaaccgccg ctcgacttct tccacaattc gcgtctgctc
    24361 ggcgagagat ggaattggaa cgggcatttt cgaaatgtca cccaaactaa aattcgcacg
    24421 tgcaacatca accgcttgag aatgaatcag cgtttgggtg gctggcgaaa gcaggtaaat
    24481 catcaagaac tgcggcaata ttttttcctg ctgacatctg attacggcca cagcttgatt
    24541 cacattacca ccggcaacat cgcttgtcgc aatggctgct cttccgatag acgcaccaac
    24601 aatattcagc agaacgtcgc caactttcaa aacggacttc ttttcctttt cattgaagcc
    24661 cgccggcaag tgggccactt cggataaatc gagttttccc cagcagacat tttggctacg
    24721 aacgaaaacc acgccgtcgt cacaatattc aaaaccttgc caattgggtg aagatccttt
    24781 tgttacaatc aaagtgacat cgcgaatggt tgtaattggc catttctctg tggggacgag
    24841 gcgtccttcg caggcggctt tgaggacggc ggcgcggtag cgtttgaggt tggcctgtac
    24901 gcgcttgagc gcgctgactc ccgcctccag tcttgtaaac tgcttctcaa tctccgccac
    24961 aatccgccgc tgttcgtcaa ggggcgcgac ggggattgga agttgcgccg cctttttgcc
    25021 tgagatttcg aggaacgttg ttccgctggc catttccacg gcgagtttct ttgcgtgatg
    25081 aaggtaatag taaacaaaat cggatgtcag accgtcctgt aagacgaagg atttgaatcc
    25141 ttggttggtt gaaacgggat tcgatgcaat cgcgacgtag ccaatcggag cacgcgaact
    25201 gaacaaaacc gcgccggctg gcattaccct tgcgccagag ttatccagac cagccttggt
    25261 aatgctcctt gcgccagccg atatgaattt ttcggtgtaa ccagacaagt ccgctggcgt
    25321 aatccatgga atgttgccgc cgaaatattc agcttggtcg ctctttggcg ttccgcctcc
    25381 gacgacagcg gcgacgtcac ccatcgtgct ccactgccag cttgctggga tttcccatgg
    25441 ttcactcatg ccgcaagaac ctcgttgagt tcatcgagca gtttgggtag ttggtcgccg
    25501 aaaagttgat gcgctttgcc caagccgccg cgctgattga acggggaaag ctccaaatct
    25561 tccggctcaa tgctgatggc cgtagcaata tggtcgcgga tgaggttgag ccagccgagc
    25621 tgttctgatg tgaaggcggt gccggattta gctttgtcca tgagccattc attaaaccgc
    25681 tcggttacgg agtcggcaaa aggcgcaagc acgggctgct gttccagcgc aaaacggacg
    25741 agagcgacca agtcggcaaa acgtccggcc tgcgagcggc ctttgacttt gccgggcgcg
    25801 gtgaccgcaa aggcgttcca gagattgtct tcggtccacg cggcgtgatt gtcgcgcagt
    25861 ttcttttcca gttccttgag catcggctcg gtgaggcgtt gtttgaatgg tcggctgtaa
    25921 agaatttgca aagcggcaat ctcggcctga tgctgttcga tgtagtcgcg gaaggatttg
    25981 agaatgtttt cggctttttc cttcgcggcg gcatcgaagt cggaaacgat gaccctgtca
    26041 atcgtggtgg tgtcgatgat ctgttcggcc tcctgcttgg ctttggtgag cgcatcgcgc
    26101 aaagcgggca gatcaaacgg cgcacaggct tcggcaacga gttcctgccg ggcggtttca
    26161 agctgttcgg gcgcgacttc ctgcggttcg gcaccgggtc cggcggcctt ttcggcgatc
    26221 ttgtcgggat tgacggcatt cagcagcgcg ttggaaagtt cagatagcga ttttccgccg
    26281 gagagttcag taagtttctt ggcttcatcg cgggaaatct cccggtcgag ccgcgccagc
    26341 cgtccggcca aggtggtgag cgtatcttcg tcgcgtttgc cgagcgccac gccaagcagc
    26401 agtttgtcga aagcaacggt gcgctgccgt tcgagcgggc gggagtcggt cttgtcgctt
    26461 tcgcacacgc cgacggcgtc aacgatgacg aagtgcgttt tggcaagcgc gtccgcgcca
    26521 ctgacagcct gcaagtcggt ggcggtcagg actcgcgtgc cgcgcccttt catctgttcg
    26581 aaataggtgc ggctcttggt gtcgcgcagg aacagcaggc attcgagcgg tttgatgtcc
    26641 gtgccggtgg cgatcatatc cacggtgacg gcgatacgga gctggggcga ggttcggaat
    26701 tcctgaatga gcgtttcgga tttttcgtag cgcttggttt cgaggttgta ggttttatag
    26761 gtgatctttt tggcgaagtc gttgcctttg ccgaatactt cgcggacgag atggacggtg
    26821 tcctcggcgt gggagtcgtc cttacagaag atgagggttt tcgggacgag agtgcgtccg
    26881 gggaacagtt ccgtgaacag cgcgtctttg taggcttgca ggatggtgcg gatttgggat
    26941 ttcactacaa cggagcgatc gaggtctttg gcatcatacg cgaggtcttc gtcgagccgt
    27001 tcccagcgcg tcttgcgggt ttctttgctg cgccggtcca catagaagcc tttttccact
    27061 ttgccgcctt gttcggtgac ttcggtgcgg atgcgataga cttcgtagcc gacgttgacg
    27121 ccgtcggcga cggcgcgctc atggttgtat tccatcacga ggttctgatt aaagaaccca
    27181 atggtctgtt tcgatggtgt ggcggtgagg ccgacgagaa aggcgtcgaa gtattcgagc
    27241 acctgccgcc agaggccgta gatggagcgg tggcattcat cgacgacaat gaagtcgaat
    27301 gcttcgagcg gaacggcggg gttataggcc acgtctttcg ggcggtcgtc agcggtggcg
    27361 atttcgtaac cggactgttc gtcgatgtct tcgtcgagtt cctcaccgcg taggatggag
    27421 taaaggcgct gtacggtgca gatggtgacg cgggcgacgg agtcgaggtt gctggaggtg
    27481 aggtgctgaa cgttatacac ctcggtgaac ttgcgtccgg tgtccggcgc gacaaactgc
    27541 tggaactcgc ccatggcctg gcgtccgaga ttggcccggt ctaccaaaaa caggacgcgc
    27601 ttcgccccgg cgtgtttgat gagccggtag gtgaagctgc acgcggtgta ggttttgccc
    27661 gcaccggtgg ccatttgtac cagcgcgcgc ggatgggcgg cggcaaacga ttcttcgaga
    27721 ttgctgatgc cttccacctg acaggcgcgg aggttttgcg caggaagcgg atgttttacc
    27781 ggcagctccg ccaaccggcg gcgcaaggtg tccggttcgg aggccagcgc cgcgagcgtt
    27841 tccgggcgat ggaacgagaa gaggcggcgg gaacggggtt ccggatcgcg ttcgtcgcgg
    27901 aagaaggttt caacgccggt ggattcgtaa aggaacggca gcatgccggg cgttaccgag
    27961 ttgatgaatt ccggcaggtt ttcagcgtag tggccggact gctcggccac gccggaaagc
    28021 agcgttcctt ccttcttggc ttcgatcacc ccgacggctt ggcgatcgac cagcaacagg
    28081 tagtcgcagg tgccggattt gagcggcgct tcgcgcaagg cgatgccgcg cccagcagaa
    28141 ggattgaact gcttgtagtc ctgaacaacc cagccgcatg caacaagctg tgcgtcgatc
    28201 tgctggcggg ctttctgttc aggcgtttcg ggcatacggc gtcctttaaa tcattaatgc
    28261 cgaggaatat gccgtccagc atggtcaatg acaagccgca aggcatctct ttccaagggt
    28321 ttcggcttcg agcagtttca taaaggcaat ctacgcgcca gaaaatcaaa cacacggtac
    28381 cgcgttgctt tgtttctgac gtatctggcg ttgggattcg gcctgtttgt acattgcaac
    28441 ttttcgccgg gttcacgccg gttcgggaca gattgccgac gactacgttt atgccgacgg
    28501 tcttggacgc aaaatccaga cccgtgccga aagcgagcgc aacgacggct cgttccgtgt
    28561 cgccagcacc gtttacgatg aaaaagggcg catcagtcag gcgtatgagc cgcgttttga
    28621 acagggcgga gcctttgttt cctttgtgcc gcttaatccg gctgttacct accagtatga
    28681 tgcgatcgga cgggttttta aaatgacgcc gcgcaacggc gacgtcggtt cgccgacggc
    28741 tccgaccagc accacgtacg ggtataacaa taatccgtgg gtgacggttg ttactgatgc
    28801 actgagtatt aagcaccggt tttttaaaaa tgccaacagc cagaccgtcc ttgtcgaaga
    28861 agatgtagaa ggacagacaa tcagaacgca gtatgtgtat gaccggctca aacgtctcgt
    28921 cacggtctct gatccggtag ccaacagcat tcacatcggc tatgacagtc tgggtaataa
    28981 gacgcagatg accgaccctg atatgggtac atggaattac ggctatgacg ccgccggacg
    29041 catgaccaat cagttggatg ccgtcggcaa ccgcattgcc atcgagtatg atgttcacgg
    29101 acgtcctctt tttaaacggg tttaccgctc cggcggagcg ctggaagaca cggtaagctt
    29161 tgcgtatgac agcggcagta ccggatttca ggtttatgac ggccagctct atcagatcac
    29221 ccatagcggg atcactaacc ggttcagcta cgatacgcat ggcaatgtcc tgaaaaccga
    29281 actgcaggcg gccggcattg gaacttatac ggccaccaac agttatgatg ctctcgaccg
    29341 tccgcttgtt gcggcctatc cggtcaacag cgccgttgtt agcaaccgtc cggcagcggt
    29401 gcgctataat tacaatgcag tcggcaacct ggactcggtc gccagtgcct acggagccgg
    29461 atcggacggt gctgagcttt acaaagtctc ttcgttcaat gaactcggac agattgaggc
    29521 ggtggaatac ggtaacggtg tgcagagccg ttatgcctat tatcccggct cgcggcgtct
    29581 gcaccagagc atgacctccc ttgaaaacgg cggagcgtat caggatttga gctacacctt
    29641 taatcagggc ggcctgatca cgtcgatcaa tgaccatctg cggagcggaa agaaatccgc
    29701 cacgttcaac gatatcaagt acgacaacct caaccggctt aaatcgctcc ggtacagcgg
    29761 cgaagtattg tccaaatcct acagttacga ccagctcggg aacattaaga agaacgacga
    29821 ctacagtctg atcaaaacct acgactatga cccgaaccgg ccccatgctg tcacttctat
    29881 cgggaacgat gagttccatt acgatgccaa cggcggcctg accaatgcgc ttgaccgtga
    29941 aatgacctac gacgcacgcg gtcgtctgtc gaaagtggtc atgaccgcca aaggccttga
    30001 agtccgctac agctatcttg acagcggcga acggctcacc aaagaggtgg tcaacctttc
    30061 caacaaccgc accaacctta ctgtctatct cggcaaggcc tatgaagaac agcgcggcgc
    30121 ggacggacag gaaacgctct gccacgtatt ctttaatacc aaacgcgtag cctcctttgc
    30181 tcctattcct gccggacaac aggtggcgtc ggtattgccg gattcagatg ccttgcttgc
    30241 caaagcgaca gcgacggcct caatcatcaa tccacaatcg aaaatcgcca atccatggac
    30301 tcgcaccttt gcccgccacg gaacccccat ccgctacgtc acctcgctgg ccgacacccg
    30361 gcttgaaaac tgcatcgtcc ggctggccgg gctgctctgt accgccgcgc tgctgacctt
    30421 cctgtgtggc agggaagcgc ctgtccgtcg tttcagagtc aaaggagtgg ttggatacgg
    30481 gagatggaaa actttagacc tcgggcctga ggcttcaggg ggagccacct tcctaaagac
    30541 tatagcccaa agcctaaagc ctctcgataa acaccatttt catctttcat ctttcatacc
    30601 tcatctttcc gcgaaaaaag tgctgtgcac tcttttttcg ctctccttct tcttccagcc
    30661 ggtctatgcg cagggagttt tccagcgcgg tgacgtcaac ggcgacggcc tggtcaatat
    30721 ggccgacgca ctgctgatcc agcaggtggt ggacggcaaa cgcgatctca agagcgactg
    30781 ggatatggct ccctttgcgg cggggttcgc caacggcgac gtcaactacg atggcgcaac
    30841 caatgcgacc gacgtacgca ttatcatgga atactgcgtc ggtctgaggg ctttgccggc
    30901 agcgccggtg gacggcagcg gaaatgaata ctcccagcgc ttcacctatt accacgacga
    30961 ccacctgggc agcaccggca tcgtcactga ccgctgcgga cagatcgtcc agcaggttac
    31021 ttatacgccg tacggcgaaa tgcgcagcga gctcaatctc ggtgcctcgg tcaactatct
    31081 ttacaccgga cagcagttcg accgcgaagt cgggctttat tactacggag cgcgctacta
    31141 cgaccagagc atcggacggt tcatcagtcc ggacacggta atcccgaacc cggcggactc
    31201 acagtcgtac aaccgctacg cctactgcct gaacaacccg attcatttta atgacccgac
    31261 agggcatgaa ccggtcgcac cgaatccacc accacctcct cctcccccac cgcccaaacc
    31321 ggaggttaag ccgccggaag tgccgccaac tccagctgaa gttaatgttg ctcggatata
    31381 cgttgagcta gggggtaccg tttccgtagc aaagcctaac ccggcaaatg tgaagcaaga
    31441 cgcaaaagat gccgcagcag tgtcggcgca tgtttatggc gacgggaaaa ccactcttcc
    31501 ttcttatatt tcacaactct ccaaagagca gttggaaaat aaggggttga atccgagcga
    31561 ttttaatcag aagaatggaa tggtcgcagg gctctactac aactcacgaa cagagcagta
    31621 tatgctggca tttcggggta cagagggttc tattaaagat atagcgactg atatcaggaa
    31681 tggtttagga tttaaaagtg gtcagtataa tagtgcggtg atgttggcga atgatattaa
    31741 aaaggctaca agtgggaatt ttatgatcac gggacactct ttaggtggcg gactggcaag
    31801 tgcggcctcc agagcaaccg gggcaaatgc aattacgttc aatgccgccg gactcaattc
    31861 cgcatataat aaaggcacat cttctattca agcatatata accagaggtg aaattctgaa
    31921 tgttgttcag gctgttacac cattgtcaaa cgctgccggg actcatataa cagccagcgc
    31981 ttcacattgg tatagtggtt cgttttctcg gcatagtata agtaactttt tgtgaggtga
    32041 gacgtgaatg gaaaatcaat gacacgaatc ggatggattg tacttctact tggcaccagt
    32101 acagcttgtg cggaatggca gccggtagca tcacgaatca atcttttttt tatgaaagca
    32161 ggtgattatt tcgagccaga actgcaagtg gaactggcgc aggcggcgga tcgcggcaat
    32221 gtttcgagga ttaaggatct ggtcaaaaaa gggggcgatg tgaatgctca gggaaaagat
    32281 ggaataacgc cacttctgtg ggccttgatt aaacaaaatg agaaaggact tcaagctctc
    32341 ctagaacagg gagccgatcc aaacattatt accacgcgcg caaacggtga gaagttctcg
    32401 gtcctgcatc ttgccgcaat tatggaaaat ccggattatc ttcggttggc gttggaacat
    32461 ggaggaaatc caaatctggt tgatcctgat agcggtaaaa cccctgttta tgaggcgata
    32521 atgaataggc gaagggaaaa tattgatctg ttgattgcag caggagccaa tttgaatttt
    32581 aaggaccgaa ctggacagac accggctatg attgctgcgg gcctaagcca atacgacgtg
    32641 gtgtatgaga tgattaaggc gggcgcagac ccaaaaatta cggataattg gaattataca
    32701 gtcgcatggt ttattgaaca aaacccgacg gatccggaag ctgagtcgta taaatggagg
    32761 aatctggtga ttgatctgct tcgagaacgc ggtatggaag ttctcccgtg gacgcctgaa
    32821 aaaggctctg ggcgcccctc gaaatgaact aaagcgtcaa ccaacaacgt taaccgtact
    32881 gtgaaatata aagccggaaa aatatcagct ccatcggcag agcgttttct tcagactgtt
    32941 aggcacgatg tttcatgaaa atagaagaaa gatatctggg gcttcttgcg tcagcgtttt
    33001 gcctactgtc tgccggagcc ggagccgcag aatgtcagtg tacgtcgccg cacgccagtg
    33061 tggactgcaa agcgccggtg tatggattcg agaccgccga taaatatcag gtttttaaac
    33121 acacatttgt tattgagaac accggctccg taacgcttga tgtcaccgga gtgaaagcct
    33181 gttgcggact gaaagccacc tgttccacaa accggattgc tccgcagatg tccgccgaag
    33241 tgacggtgca gtttcccctg tccggccgca cgggagcgca ggataaaatg atccgggtgt
    33301 tttccagtga tcctcacaca ccggaatatc atctgtttat gaggggaatg gttaaagcgg
    33361 acgatactac ccgcacggat ataagaaatg taaatttcgg attgatcaga ccgggtgcaa
    33421 ccgtggtcac caatctgata atccacggtc tggtgaacag caaccgggtg tcggtcagta
    33481 cgccggactt ttcggttgaa cagattgtca caacagaaac aacccagaca gtgataaccg
    33541 taaaaggcac tgccggggcg cagcctcgac tcctttatgg cgttttgcag atcagaggtg
    33601 agcagacggg aaattctgct gttgaaatac cgcttactgc cgccgtcggc tggccggtta
    33661 tggtatcccc tgaccgcatc acagtaaccc cggaggaggc tgaacagccc gtctgccgcg
    33721 tgcttttttt acagtcccgc gatcaagcac cggtcaaaat actaaaagcc gctgtatccg
    33781 gaaatgaagg gcaggttcgt attgtgacga tacagcccga ccaatgccgg ctggtggtta
    33841 aaatagcagc caatgcaggg aatccgccgc cggaactgcg gattgatgca gaacataacg
    33901 gcgaacagct tcatataagt gttcctgttt cggtcagaaa atccggcgag taacatgcca
    33961 tgaatgccgc gataaaaaca ttgcttttga ttgttgtaaa cggtcgtgct gacggagtct
    34021 tttctttcac tggctgtgct gaatattctt ttctgacccg aaaaaagatg tcccgaaacg
    34081 attttaaata aaatttaatg gctgcaaaat agctacattt gctgatgcgc ggatcatttg
    34141 gagaaagcaa aactcttttt aatatgttta tgcagagatt ttaatgtgga gccaatgatc
    34201 gggatcgaac cgatgacctg ctcattacga atccacattt taaaaactgt aacctcatga
    34261 taaccaatgt tttcaggggt tttgtgacta ctagtttgct ctcggaatcg aaaagaaaca
    34321 ctcagcaacg tagcaaaaac cgtagcaaaa tttgttcatt agaacattgc cttccggata
    34381 tgccgcctca gctgttctca cccggggttc cagttatcgc tcacagagcg atatccgaga
    34441 tcgatcgggt agtttctttc tctcaaaaca ctcaaagagt atccgtattt tcatctggac
    34501 tgcaaaaggc aaaaggagtg ttcttcgtct gatcagcgat ggtatttctg aatggcctag
    34561 ttgcagtaac ttttaaatgt tttcgtattg ggtcagtcat aacgacaata ggaattgtga
    34621 atggatgtaa ccaatgacac ataatgggtc cggcgggaaa aatacagtcc gatggtcggg
    34681 taaaaatgcc gtttggttga taattggatt tacgtttcta ttttttgtcg ttggggtggt
    34741 tctataccgt acttcaccta atcgacacca aaaatctgat gatctggctc taccagctac
    34801 ctcacttgcc gataaaccgc atcagccgtt gtcccgcatt gttgtggaga caaatacgga
    34861 tatttccgat gatcgaccgg caacactgac cgcagagctt ttgttttctg agcccgggca
    34921 catcagtggt aagcgagctt gggattgtct cgcagaattg aaagctctcg gagagtcgac
    34981 aagggaagcg atcaacgaac tttctagttc aacaaatgtg aatgcgcgat tgcttggggt
    35041 atttctcact ctggaagtat ttggtccgac taattcggtg ctcgaaatcg ccgtgcacga
    35101 ccctgagcct gtgattcctg ctgaagttgg acaatggttg tatctaaaca atcgcttcga
    35161 ccaatgggac tcttttctaa ataactatgc gacgaatcag caacctagcc agatggagtc
    35221 acttctcggg ctatgtgatc gtgttcccgt aagcgtggaa gtgcccgcag ggatgtctat
    35281 tttgacgatt ggaagggggc tcccgttatt gttgcaggaa atgtcgcgtc gcagtgactt
    35341 ggtcgcgcag gaaatatcat gccgcattct cgaccctagc gtagccgaca acaatcgtct
    35401 gtctttgttt aatgttatgc aggaggctag gccagctggt ttcgctgcca tactgactaa
    35461 tcttgtttct cagagcaccc agtattctcc actccggttc aaagcagtgt ggagcttggg
    35521 cgagacgtgc gatgataaag agactctcgc gctct
//
LOCUS       DAOPZO010000005        35013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_1140_length_35013_cov_9.013473, whole genome shotgun sequence.
ACCESSION   DAOPZO010000005 DAOPZO010000000
VERSION     DAOPZO010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 35013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35013
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1140_length_35013_cov_9.013473"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..980)
                     /locus_tag="PKI68_00680"
     CDS             complement(96..980)
                     /locus_tag="PKI68_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNX52238.1"
                     /translation="MNYVKQLLEMPKNKNFFIGFDSDGCVFDTMELKHKECFCPAFIK
                     HLKLQPVSKAAREVWDFVNLYSKTRGCNRFFAVLYACDLLKEREDIRVRGFKVPEFNE
                     LREWVRRETKLGNPALELECKKTGSEELKIALEWSKEVNARVEDMVSGISPFPFVMDV
                     LRQGSRQADMIVVSQTPLDALTREWKENKMDGYIGMIAGQEHGTKAEHIRFATEGKGY
                     APENILMVGDAPGDYQAAQKNNALYFPIVPGREDVSWKRLAQEGLDRFFNGTFAGAYQ
                     QGLVDEFNAALPEHPSWN"
     gene            1438..5256
                     /locus_tag="PKI68_00685"
     CDS             1438..5256
                     /locus_tag="PKI68_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNX52239.1"
                     /translation="MKKLLILGLLLSVGLNAKAALPPEIENPECLGVNKLPYHATLMP
                     YATLQQALAGKRLESPFCQLLNGTWKFNWVKHPDERPLTFSEPSFDVSGWKDITVPSC
                     WQVLGYGTPYYRNSGYILKMDFPRVMTEPPKDFTAYLERNPVGSYRRDFDLPADWSGR
                     RIFLAFSGVDSAFFVWINGQKVGYSVNSRNTAEFDVTPFVKPGRNMIAVEVYRFSSGT
                     WFEDQDMWRLSGIFRDCYLWSSPQVHIRDFFALPDLDAQYQDATLAVTAKVKNYADQA
                     AEARTLTAAVYDAAGKIVAQAAGEVPALAAGAEAPVAMQLKVANPAKWTAETPNLYTT
                     VLTLGNQTEILSTRTGFRKVEIKNRVYQINGVPVKLKGVNRHEMDPNTGHTISEASMI
                     RDIELMKQGNCNFVRTSHYTDDLRWYELCDEYGLYVCAEANVETHGYGFREESLSRRK
                     DAEAAHVDRNIANVENLKNYASVVMWSLGNESGPGPSFLSALKAVKAIDTSRPTHYEG
                     FAIGPANPADVDSRMYPTIAETEKAALNPSYTKPFFLCEYAHAMNNSMGALGEYNDVF
                     DKYPTIMGGAIWEWQDQGLWNRRDTNRVFLAYGGGFGDVPNNNYFIHKGVIFADRSPK
                     PHYPEMKKVYQCIGIGEDDLAAGKVKIRNKYSFLPLDRFNAEWTVTGDGQEIARGALP
                     KLPIAPGTEQTVTLPLPVISAKPGVEYFLRLSFKLARDEQWAKAGHEIAWQQFKLPVA
                     APAPAADPAALKPVALQDKGEELAVTGAGFAVRFDKTTGAIRAMERDGVNLLAANGGP
                     ALHLWRAAHQIDDKWAVADWQRYGLQKASVKLVSFSAQQVAPSAVRVIVSTEMQGAAN
                     FMVSHSAAYTIYGDGSIAVDNSVVPQGKVIALARMGVRLQLNQQLDQFTYFGRGPMEN
                     YADRKRGSDVGLYSSSVRENMTPYSKPMECGNHEDVRWAAVSGKGLPALLAQAESGEV
                     LQASALPYTDEQMDPAAYSVDLPPSKASVLVLAVKTTGVGSGSCGPRPAADCVVMSTP
                     ISFSYVLRMLPAGAALPQAGRQPAPADRVKPVLVEQAPSGDVTLTCATAGAKMEYAVD
                     GAAWQPYTAPFAVTHAAKISVRATAEKLTPYEAQISLKALDYRRGWTLTASSFQRDLG
                     TTSYAVDGNPETSWSSIWSRGPMPYPHWLSVDFGQPLTLSEVSCLPPQGSRNVQRIKD
                     YELYLSNDGKNWGSPAAKGSFKNTGDRQVIRLAAPATCRYLKLVALSGMDGRIMASIA
                     ELDVQIVESK"
     gene            complement(5275..6072)
                     /locus_tag="PKI68_00690"
     CDS             complement(5275..6072)
                     /locus_tag="PKI68_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNX52240.1"
                     /translation="MSRNREIEREQIVHGNRWGTFHDGYFSNADVARPLLEQVIRCAG
                     LSGAATVVDLGGGTGTLSRLLTEQFAPLPFRLVTMDGSSEQLKTAQAAGVTTHQGSIE
                     TFRRADLNPDGGPLLYMMRSVLHYLGREALAGALEHIRRQTGPDEYFVHQTACFSSQE
                     EADCLNELYARMRTEKWYPAENRLTELLQQTGWSVEAILPAPALALTAEDLSLRYALS
                     AQDRLEIRDRICAAHPACGHLFQPDATGFTAYLPYRIFICRNRAASR"
     assembly_gap    6128..6227
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6228..7532)
                     /locus_tag="PKI68_00695"
     CDS             complement(<6228..7532)
                     /locus_tag="PKI68_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNX52241.1"
                     /translation="MKKLFMAMSWMLLAGTAARTEETANGAFQPTWESLQNYQCPDWF
                     KDAKFGIWAHWGPQCEPGKGDARNMYVEGHPSYKGHLEKYGHPSEFGFKDVCNVWKAD
                     QFDPERLIGLYKRAGAQYFVMMGNHHDNFDNWNSKYQPWNSVNVGPKKDLVGLWEQSA
                     RKAGLRFGVSIHAARTWEWYEVSQLSDKSGPKAGVPYDGNLTAADGKGKWWEGLDPQD
                     LYAQRHKPSSDPADRNNTNRIPGEPPSKAYCDKFYNRVIDLIDSYKPDLLYFDDTGMP
                     LQKEGAAYGLGIAAHLYNVSMQSHAGRNEAVMNGKKLNALQRKCLVLDIERGKSDRID
                     PLVWQTDTCIGKWHYRDSIFENHQYKKPAEVIHMLADIVSKNGNLLLSIPVRGNGTLD
                     ADEEALLEELAAWMPVNGEAIFGTRPWTVYGEGPSMFVQAEAS"
     gene            7740..9485
                     /locus_tag="PKI68_00700"
     CDS             7740..9485
                     /locus_tag="PKI68_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNX52242.1"
                     /translation="MKNLRTAVIGSSCALAMSLFAEEQKPNIILMYVDDLDFDQISAY
                     ADSEFPCYTGAKNTGNLKELSPVTANENGQFLVQGEMYYYKNPTMLTPNIDRLAAGGM
                     KFNRFYVTSSICTPSRYSMLTGRYASRSPQLLRETPPGTTPLIGWNSFMDPDESNLAR
                     SLKSAGYVTGLVGKWHLSSYELEGIDFGAGFDSYRTRQENRKQVLPHQMVGTYFPLTA
                     DYGDPAVQTEVKRIYEIMSKKVLTTSGFDVVDRLYFANYGELPIPLHMKVHNLEWETE
                     GALDFIDANKDRPFFLYFAITLPHGSYTEDWMSKDWRATPAGMLSSPPRGMPSRDSVR
                     ARVEAAGLPPQNAMATWIDDSLGAILNKLDEAKLSGNTIVLFISDNQSRGKLTAYEGH
                     RVPAIILWPRHTPAGSVEERIVANIDIRPTLLEIAGVRPADNETVDGKSLVSILEGKI
                     PPAWRDSLFLETAYSRAVITKDWKYIANRPPPEVVAKMEEDRLAAEKEGRRRHVDWSG
                     RATDPSSGLGVRFNADQDFPNYFDSDQLYNLKDDVFEQRNLFSNPEYKATASELHTLL
                     GQYLKNMPRGFGEFR"
     gene            complement(9510..10376)
                     /locus_tag="PKI68_00705"
     CDS             complement(9510..10376)
                     /locus_tag="PKI68_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNX52243.1"
                     /translation="MEKEPSFISTQVSAGDYYYLNLSPGKDVKAAIVCGGREQCAPDY
                     QVDRDGFKFCSIEFVSAGKGILTLQEETYPLQPGAVFFYGPGIPHRIKTDPQAPLVKH
                     FIDFTGQELKTLVERTRFFQRPLYATHPIKILSVFENLLQTGRTDSRHQKALCLLLLK
                     QLILHIDDSSMPHQEVLSPAWQTYLRCRGLLEQNFRRLKNIEQAAAACRLDKAYLCRL
                     FQRYASETPLQLLTRLKMNRAAELLSGRTMLIKQIAQESGYSDPYHFSRVFKRAYGIP
                     PESFQQTVRRQM"
     gene            10512..11561
                     /locus_tag="PKI68_00710"
     CDS             10512..11561
                     /locus_tag="PKI68_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNX52244.1"
                     /translation="MTDVKKTKFTVAKDAVDPNSIPKKKLNNGTSMPAIGMGTFGSDR
                     FTPVQVANAVIGAAEYGQRAFDCASVYGNEKEIGASLKVIMDGGVPREELYITSKVWN
                     DMHGEGNVIKSCKQSLKDLGLDYLDLYLVHWPFPNFHPIGCTVDSRSPDAKPYIHENY
                     MKTWAQMEQLVEMGLVKSIGTSNMTIAKMKLLLRDCKIKPVVNEMELHPHFQQPELFD
                     FMVANGIQPIGFCPIGSPTRPDRDKTATDTVDIEDPVIKKIAARLGVHPAVVCIKWAV
                     QRGQVPIPFSVFEPEYRSNLRCALPEYMTVTGAEMAELAKIDKNCRLIKGQVFLWKDG
                     QTWEDLWDLDGTIAK"
     gene            11635..13041
                     /gene="fucP"
                     /locus_tag="PKI68_00715"
     CDS             11635..13041
                     /gene="fucP"
                     /locus_tag="PKI68_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HNX52245.1"
                     /translation="MHDTDQKVPVVSKALLGSFILVTSLFALWGFANDITNPLVRAFK
                     EVFPGLTNAQSSWVQGAFYGGYATMAIPAALFIRRFSYKAGILLGLALYATGALLFIP
                     AAVTKQFVWFPISLYVLTFGLAFLETTANPYILALGDEKTATRRLNLAQAFNPIGSLI
                     GMFVASKFILAKLWVTDFRNAEMAAHPEYNDLLPSVVDAKIAAAREAFAAANPAQFIG
                     NQTHDLNVIRNPYAAIGIVVLIFFVMFLVKKMPVLKDDGGSIHPWDTAKQLFSIPRWW
                     SGVIAQMFYVAAQITCWTYVIHYAMTNVGMSMDVAQNYNIAAMALFLISRFICTFLLK
                     YFSPGHLLMTLSVVAIGLMLGVIFLQNYTGLLCLIGVSGCMSLMFPTIYGIALDGMGQ
                     EAKLASAGLVFAIVGGAWMPLVMGGIIDHPDFDLGFVKLTSVSMSFVLPLISFVVTAL
                     YGYLTFKIFRPASELTRK"
     gene            13057..13488
                     /gene="fucU"
                     /locus_tag="PKI68_00720"
     CDS             13057..13488
                     /gene="fucU"
                     /locus_tag="PKI68_00720"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: protein motif:HMM:NF011949.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HNX52246.1"
                     /translation="MLKGIDPIICPELLKIMAEMGHGDELILADAHFPGNTFGKKVLR
                     GDGLQIPALLEGIIPLFELDSYADPLIMMAAVEGDKLDPKVEKSYMAAIRKHAPEAPA
                     PVRIGRFDFYDRAKNAFAVVMTGTTAKYGNIILKKGVTPLA"
     gene            13498..14127
                     /locus_tag="PKI68_00725"
     CDS             13498..14127
                     /locus_tag="PKI68_00725"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNX52247.1"
                     /translation="MAAIKTLYFMRHARSEANLKDILASRLDFPLTEEGRRDAEAIAR
                     EFAAVASVDRIVSSPLIRARQTARPFAEACGLTVQTDERLTEQHLGIFSGLTYAELDT
                     RPDYMHDRAQRWTWVPAGGGESYQMIADRLRPFFLDLESMTGSSILFVTHAVTMRLIR
                     ATLEQTLPEYPQTIAKNGEIWKVSYTQYGAPHRVESIFLGGSAAAVSRA"
     gene            14145..15644
                     /locus_tag="PKI68_00730"
     CDS             14145..15644
                     /locus_tag="PKI68_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNX52248.1"
                     /translation="MAEYILSIDLGTTSCKAVLFDTDFNIVTTARREYKTSYPHAGWA
                     EQPAYQWWEALRENTKAVLKQSAVDPAKIIAVGIDAFSTTVLPVDKAGDPLRPGLIWM
                     DRRSGKQSDWIAKNLGKELWEINGNVSDAGNIAPKIMWIKENEPEVYKKTHMFLHANG
                     YLVYCLTGKYSMDISEAGLSQLCNTATGQYSDELINACGIDRAKLPPIFNCTDVVGKV
                     TAEAAAKTGLVAGTPVIAGSMDNVAAGLGAGVSKGGEVFISGGTVTTNNVCLSVPKYN
                     RNLHIYPHIVPGTWITAGGVDFGGAGLKWFKELLEVENYAEIDALADTSVCAKSSVIF
                     LPYMVGQRCPIWNDHTTGVLIGLKPTTNRQDLVRAFMEGTTYGSRHVLSLVQDEGVAI
                     ENVKITGGSANSAAWVQIFSDVIGKPIDIPGAVDLPPLGVAIAAAYGVGAIKTFDEGI
                     AKIAIRESFVPDEENHAYYTEMYGVFRSLYENIKGEYDRLAAIERKFKK"
     gene            15658..16692
                     /locus_tag="PKI68_00735"
     CDS             15658..16692
                     /locus_tag="PKI68_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HNX52249.1"
                     /translation="MRAALRKGQKKVEVIEMAKPAPLADEVLVAVKSCGICGSDIVRI
                     QEENPRWDEVVLGHEFAGQIVEVGANVKEWKAGDRVSAAPLMPCHQCAHCAQGNFSLC
                     KGYSFIGSRVQGAFGEFLRVPARNLVAIGDLTYDQAAFIEPITVCLHPILRLGNLLGK
                     DVVVTGAGTIGLLAIQIFKAMGCRNIVASDISEGKLEMAKSMGANILCNPIKESLENV
                     CARELKDGAHVVFESSGAGPAKISAMAIARGGGTVLLVGTAHAPITFTGPQFEQISRK
                     ELHVVGSWMNYSAPFPGTEWSTAAWMMSAGLIKVDALQTHTFPIEKIQQAYDVIYTNK
                     ELWAKVMIHF"
     gene            16708..18024
                     /locus_tag="PKI68_00740"
     CDS             16708..18024
                     /locus_tag="PKI68_00740"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR02810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-tagatose-bisphosphate aldolase, class II,
                     non-catalytic subunit"
                     /protein_id="HNX52250.1"
                     /translation="MSAIEKFVQILQDNRAGKATGIYSICSAQETVLQASMLQAKKDG
                     SILLIESTSNQVDQFGGYTGMKPADFVAYVRGIAASVGFDAQNLLLGGDHLGPNAWQK
                     EPAASAMAKAHDLIREYVKAGYQKIHLDASMFLADDQGDRHKALANEIVAERAAALCK
                     TAEETHAKYCAGQPKPVYIIGTEVPIPGGAQEAEETVTPTSPADAVQTIEVTRAAFEK
                     AGLTEAWNRVCGVVVQPGVEFGDDQVFHYQRSKARGLSEKIMQFDRFVYEAHSTDYQS
                     ETGLTQLVEDHFCILKVGPWVTFGYREALFALAQIEEELLAPKGLQTSRLREVIDAVM
                     VEDTKYWAKYYPGNATEQAYKRKYSFSDRSRYYWPNPRITAAKAQLVANLKAHPVPLS
                     LLSQYMPGQYRAVSEGLIRNDVEEIIIHRTQDVISLYARACNMARR"
     gene            18037..19032
                     /locus_tag="PKI68_00745"
     CDS             18037..19032
                     /locus_tag="PKI68_00745"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNX52251.1"
                     /translation="MNLARMLDVLVICVPVFAVLGLGKLLQRNGRLDDGHCNFINWLV
                     YYFSLPALIFTTVARQRFEGFLDPALILMPLLALILVIAVTMLIAKLSRFKGGFAAAF
                     VFGTFWANATYLGLPLCTNAFGAEGLAKAAVYNACVVPFFIIFGYSLLGFYGAGSGDL
                     KIGSRIRTALLNPVLLAAVAGILVALSAGLFRSADGALNLPVPVLSAATLLGSFFQLV
                     GSMGLPLGLLSIGASLRWEQARGHLVALSWTVGCKLILLPLLTLLLIRRFCPSADAVS
                     LGSAVILSATPGAVACYIISCKFGIEKSFAAALLVVGTALSVLTIPCWVYLLKSF"
     gene            complement(19120..19854)
                     /locus_tag="PKI68_00750"
     CDS             complement(19120..19854)
                     /locus_tag="PKI68_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNX52252.1"
                     /translation="MKKIRLDQLLVEKGLAESREKARRLIVAGQVLVDGQPSPKPGQT
                     LSPDREIVLKEVERFVSRGGEKLEGAMEAFKLDLAGKVCIDIGSSTGGFTDCMLQHGA
                     TKVYAVDVGKGQLHWKLREDPRVTVMEGVNARYLTPADLPEAADFASIDTSFISLTNI
                     LPAVKGLLKPGGEIVSLIKPQFEAGKEEVDKGRGVITDPEIHEAVIAKIRTFGTETLK
                     LEWLGLATSPLKGPKGNVEFLAHWRA"
     gene            19900..20961
                     /gene="hemW"
                     /locus_tag="PKI68_00755"
     CDS             19900..20961
                     /gene="hemW"
                     /locus_tag="PKI68_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNX52253.1"
                     /translation="MAGLYIHIPFCERKCSYCAFYSFAKPWKNVAGFFQALGKELNSL
                     PADFSPETVFIGGGTPTALDLKTLGSFLAMLRKFSPVEFSCEANPGTVDRKKLELLIK
                     NGVNRLSIGVQSFNSKCLEMLGRIHTAEEAEAAFRLAQTAGFKNIGLDLMFGVPGQTI
                     QMLEADIERALALEPEHISIYNLMYEEGTPLAVRDPERLDEGLEREMYDLIRRQLAAA
                     GFIHYEISNFAKPGFECRHNLLYWSGGEYIGLGPAAHSHWRGMRRANVSDVEDYCAGG
                     PRREFEEILDPEAKERETLVMGLRRLAGVEVSVDLFENKKKTFLALEKEGLLVISGRH
                     VRLSEEALFVSDAVFSELV"
     gene            21043..21270
                     /gene="rpmB"
                     /locus_tag="PKI68_00760"
     CDS             21043..21270
                     /gene="rpmB"
                     /locus_tag="PKI68_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNX52254.1"
                     /translation="MAKCAMTGKGTISGRRIVRKGLRKCKGGIGLHVTSATKRKFKAN
                     LQHVRIKDENGTVRRVWVSTKAIRDGLVTKA"
     gene            complement(21442..21747)
                     /gene="rpsN"
                     /locus_tag="PKI68_00765"
     CDS             complement(21442..21747)
                     /gene="rpsN"
                     /locus_tag="PKI68_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNX52255.1"
                     /translation="MAKTSSIVKNEKRMKLAKKHFAKRMELKEIIHNPKTSMEDRMAA
                     FDKLRKLPRNANPIRVQSRCRVTGRPRAVYKKFGLSRITLREMASNGLIPGMTKASW"
     gene            21803..21991
                     /locus_tag="PKI68_00770"
     CDS             21803..21991
                     /locus_tag="PKI68_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52256.1"
                     /translation="MVQPFSMQNSPVIGRSACCFKFCFRGFFRQASFRFLDFPGGGID
                     VRSSLSGESGKIAGGEAD"
     gene            22113..23411
                     /locus_tag="PKI68_00775"
     CDS             22113..23411
                     /locus_tag="PKI68_00775"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNX52257.1"
                     /translation="MKLDKVGQAEIHIDDRTVVCPVYEGSEGERAIDIGKLRKETGAI
                     TYDPGYGNTGCCASDITFIDGEKGILRYRGYDVADLAEKCEFIEVAYLLVHGSLPTHA
                     QRENYATLLNSHSMIHEDMREFFSNYPEHSHPMAILSAMAVSLSSFYPELRGDLKEDV
                     DMTVTRLISKLRTIAAFSYKKSIGEPAVYPRYDLKYCENFLNMMFSSPVRGEPIDPVM
                     ADALNKLLIIHADHEQNCSTSVVRSVGSSGINLYASIAAGICALWGPKHGGANQHVIE
                     MLEEIHRDGGDVDKVIERAKNPRSPFRLMGFGHRVYKSYDPRAKIAKELCKKVIAATH
                     TRDPLMDIAMELEEKALADSYFQERNLYPNVDFYTGLTYREMGIPTNMFTVMFAIGRL
                     PGWIAQWLEMQNDSDARIARPRQIYTGPNLRKVTPIAKRK"
     gene            23426..23842
                     /locus_tag="PKI68_00780"
     CDS             23426..23842
                     /locus_tag="PKI68_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52258.1"
                     /translation="MIPTDQQPETLRENKQELEFLQAIARRLPEDTDVLRALADLYTR
                     TGSYADGLRMDERLSRLCAEDPLVWYNLACSLALSDRADDAFDALNRAVELGYNDYEW
                     MKKDPDLAALHGDARFESFLEWIYSAFEQTPDCDGL"
     gene            23928..24104
                     /locus_tag="PKI68_00785"
     CDS             23928..24104
                     /locus_tag="PKI68_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52259.1"
                     /translation="MNKSLLWALFLIALCALFFIFTGGKTTIRIFELDYTMKTPIALL
                     IFTAIGTAIGVLLK"
     gene            24101..25192
                     /locus_tag="PKI68_00790"
     CDS             24101..25192
                     /locus_tag="PKI68_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52260.1"
                     /translation="MTDAFSRRGVFIGPEVNPARIAPDAEVYPGCRIRGAKTSIGPGS
                     VLGAEAPVTIENCQLGRDVQLKGGFCSGAVFLDGAGMGSGAHVREGTIFEEEANAAHT
                     VGLKQTVLLPFVTLGSLINFCDILMAGGTGRKNHSEVGSSYVHFNFTPHQDKATASLV
                     GDVPRGVLLNQKPIFLGGQGGLVGPARIAYGSVVAAGGICREDVVEENQLHIPAVPAE
                     KTVPFETGVYKNIDRIVKNNLIYIGNICALKAWYENVRVFFIRDEFDRACHEGALANL
                     DLIRKERVQRLEQLVGKVGDLSRSWKKICVRLKEPPRTGADAQLMSEIKKADKADYIA
                     AIQNLSEDACAGTTAWLQGIVDETAVLDG"
     gene            25460..26776
                     /locus_tag="PKI68_00795"
     CDS             25460..26776
                     /locus_tag="PKI68_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52261.1"
                     /translation="MKRTFLKGFIFLTAAVLYGTAAASSLFPFYDNLTTEQGSFFAVR
                     PFYSHTVVKEGQIRDCFWPLYSRKDFKDEQSSRALIFWFTHKFDAKDASPRDRNWLLP
                     VYFQGRDVNGKGYFALFPLGGTIKEFLGRDEIMFVLFPVYGQSRINDVKTTSVFWPIY
                     SHTRGEGIQRDRVFPIYGKSVLEGKYDKRFVLWPFWTSAEYFYPGNAGKSWILFPVCG
                     RSKLEQEKTLWVIPPFFRFTKGEKQDRLFCPWPFIQKTDSKPHSKFYIWPLWGEDRYA
                     AGPTQRTFLLWPLLWSEKTGSDSLLRTHRMALPFFYQDYSVQREKGVSPEKRTTVSNY
                     WKIWPLMSWQREGDVSRFRMLELWPLKNSAPVERNWAPLWTLYKRTDMEGIIRKDILW
                     FAWHSEKEPSVERKEWSLLKGFLAYKRDHESQELRLLYFIHLGNSE"
     gene            26773..27594
                     /locus_tag="PKI68_00800"
     CDS             26773..27594
                     /locus_tag="PKI68_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX52262.1"
                     /translation="MIAPFKEIYWYLDLPVRSFRRLGRIILVACHDMGNALIMLFSAT
                     LYIRTLWKPRARRETAMQLYVTGIKSLGVITVVAFFTGMILALQTGLELRRFGQEVNI
                     GTAVTIVMIREMGPFMTGLIIAASVGSAIAAQLGTMAVSEEISALEVMSINPNRFLVM
                     PRLVALAVMMPILTVYTNILGILGGGIVGSTQLGVAFQAYLDNATRYAENKDLYVGLF
                     KAFVFGIIIATVACYQGLSTTEGAVGVGRATRQTVIISFLMILIIGYMITRLFYI"
     gene            27591..28361
                     /locus_tag="PKI68_00805"
     CDS             27591..28361
                     /locus_tag="PKI68_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX52263.1"
                     /translation="MIIFDKVTKRLGGKTVLNEVSFEVPKGETFVIVGLSGAGKSVTL
                     KHMVQLMVPDSGTVRIGDDIISAARGTELERMRRRFGVLFQGAALLQWLTVFENVALP
                     LREHTKLSEEEIDRTVREKLRLLNMEDAMDKFPADLSGGMQKRVGLARAIVMNPEIIL
                     YDEPTSGLDPVTSRLIDKLIENLRKELGITSVVVTHDLHSALAIATRIMMIDGGEIVE
                     NATPAEFIQSTNETVQRFLESQYITRRGAWEKGMEQHV"
     gene            28354..29286
                     /locus_tag="PKI68_00810"
     CDS             28354..29286
                     /locus_tag="PKI68_00810"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNX52264.1"
                     /translation="MSDKYNKELSIEVLVGFFMFIILIALGVFTIILSRQNFLQKKYP
                     LAVRFSEVSGLRDGDNVFLRGMKVGVVKTTALEDHHVVVRADLEVPVQLREGYKVEVV
                     SSSMLGGKQLKIDEGPMSASLLPENAPIIGTEPTDILEELGAAVAGIRQMTDKISAGE
                     GTLGKLIHDDAVYNNLQESLANLRDVSDKLAKGQGTIGKLINDETVYTDLQSTMASLK
                     DVSGRLANGEGTLGKLLSKDEQLYQDLSTTMANLRSITDKVGSGEGTLGKLVNDDQIY
                     TEAQKLLGELRAAIDDMRETSPVTTFSTIFFGAF"
     gene            29397..30182
                     /locus_tag="PKI68_00815"
     CDS             29397..30182
                     /locus_tag="PKI68_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNX52265.1"
                     /translation="MNSSASENEIYPACGLQVDRPLKIRVLQYASLVLAVAGLILLYL
                     FAVNRDLPFVRIGDITPAMNFAYVRISGEVTRDAYVFQSGGIVFDLKDGSGEIAVMGG
                     KAQAQALEAAGKLPRRGDRIEVAGSLSVAADQEPRLRIQSADQLVLNRKRAAAASSPA
                     PRIRLADVTAAHKGEQISVSGILKIIDVPGPGSRAPYTLTLEENGAELAVVFWEDVFR
                     DLEDRLPLPGKRLSVRGGVDVYKDTVQIKVRSAGDLRIAESGQ"
     gene            30179..31507
                     /locus_tag="PKI68_00820"
     CDS             30179..31507
                     /locus_tag="PKI68_00820"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HNX52266.1"
                     /translation="MIFTAVAAVLAGTVLACFLSILPGLHIYNVMGLAALFLYSLQGS
                     GVAAMPEVYIPFMLGLVTGWSVLNTIPSVLLGAPDESAVFTVLPGQKYLMTGRGYEGT
                     MMIGAGSLAGLFILVFLIGPFAPKFLPIVRYVLTPHMHWILWVIILFMLMTEWPKNGN
                     WGPAGWRKFFAAWSTLGAGLLTFFLSGLLGFLLLYRPPVSVDNAFQNIMPAFVGLFAV
                     PWCLLNAVSGTEVPKQYIRDTLNINGDLLLRSGIAGGIGGGFAAFLPVVTGGIGGLLA
                     GHATAQRDERVFMMSQGVSKVVYYVGALMLFFVPGLYLTRGGGAWIIKGLYTPHTRGD
                     YCLALGSIALSGAVSFLLLSPLTRWTLKLMGKVDYRHISVFSLGIIVALVFSITGRPG
                     LFIMTVSTGIGLIPVLFGSRRLNCLGVLLLPIACNMSGIGEPVAAFLGLL"
     gene            31509..32489
                     /locus_tag="PKI68_00825"
     CDS             31509..32489
                     /locus_tag="PKI68_00825"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNX52267.1"
                     /translation="MKGIAHFISGAAVASFCPWAVEAAQNGNPAFFILGAAAGILPDT
                     LDFKFYRFFYRYDVSVYPDPEKLDPQPVADAVAAAVSEAAATGGPVRVKLSTIKLSAD
                     NWRQYRVRIDPDAGEVRAQFGPVVSTGQAPQPGTLPASRPAAVAKFSAPVRQSYEPVY
                     TVDIFDGPGFLFKRNGAGEVEIDFLPWHRSWSHSFTVASLLAGLASLWTWEAGLVVFG
                     AYAAHIIEDQMGYMGTNLFFPFTKKRTAGLKLMHSGDAFPNFTCVWLCGLLIFWNIYA
                     AIPEPLYHFSFMRFMTYALIIPFAVFGAVRRFLVRAGNGRPESGAVKWDI"
     gene            complement(32529..33476)
                     /locus_tag="PKI68_00830"
     CDS             complement(32529..33476)
                     /locus_tag="PKI68_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNX52268.1"
                     /translation="MIMTYRIASARLMQCAENEADIFVYCQPTDPEKEALQTKFDLDP
                     FDVEAIYDSDEVPRVEYMQDGGLFMIWKRPDNITRDETIQFEVSSLGILIKDNKVVFI
                     VPQDDLPISGREFKQIGSVWDCVLRVLLHTVHHYQGHLKAIKMMSQELQAKIIKSMEN
                     KYLLQMFALGESLIYYHNALETNLAVLSKIHTASGKLKLSTEQIEFLDDVIIENQQAS
                     KQAGIYSTVLSGLMDARGTIVSNNMNVLLKNLTLINVVFVPINIIASMGGMSEFSAMT
                     KNINPWISYGLFSVAMVVIGWGLWWWLTKVLDHLQNRSS"
     gene            complement(33608..34195)
                     /locus_tag="PKI68_00835"
     CDS             complement(33608..34195)
                     /locus_tag="PKI68_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52269.1"
                     /translation="MTDLFVKADGGIVWLIVGVFWVIAQIAGAAAKKNQPPRPVAEEE
                     ETGAATEDPFAELLRKMAGAENLHIEPPVFIEEPQHKPAFRSSSPWKPGDIEALPDIE
                     PLRHEAPAPEPAAKPAALPEPDLRPKMSAFRNSVPSIKLPTMNLSFQGSETSVRKSSD
                     LGKILNPSDRNSLRRAMLSHIIFSAPKALEQTGKQ"
     gene            complement(34198..>35013)
                     /gene="floA"
                     /locus_tag="PKI68_00840"
     CDS             complement(34198..>35013)
                     /gene="floA"
                     /locus_tag="PKI68_00840"
                     /inference="COORDINATES: protein motif:HMM:NF010186.0"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HNX52270.1"
                     /translation="GIDISSDLLEAHYLARGNVVNVVQALIAADKANIELSFQQAAAI
                     DLAGRDILDAVTTSVNPKVIDCPNPKQGTTFLDAVAKDGIRLLIKARVTVRTNLEQLV
                     GGATEETIIARVGQGIVSAVGSMDSYKKVLENPDNISRKVLDSGLDAQTAFEIVSIDI
                     ADVSVAGTTNTANVGAMLETERAEADKKLRQAEAEGRRAMAIAQEQEMRARVQEMQAK
                     VIEAQAQVPLAIAQAFKEGNLGVMDFYRMQNVMADTSMREALAGGDEQAHKKE"
BASE COUNT         7268 a         9678 c        10105 g         7862 t
ORIGIN      
        1 gttttccgga cacgaagaaa gggagaattt tatttctccc tttcttcgtg tcggtctgcc
       61 gacgttgttt cagaaactta tttcggccgg acggttcaat tccacgaagg atgttccggc
      121 aaagccgcat tgaactcatc tacaagccct tgctggtaag ccccggcaaa cgttccgttg
      181 aagaatcgat ccaggccttc ctgagccaaa cgcttccatg aaacatcttc ccggcccggc
      241 acaatgggaa aatacaatgc gttatttttc tgtgcggctt gatagtcacc gggagcgtcg
      301 cccaccatga gtatgttttc cggtgcgtat cctttgcctt ccgtcgcaaa gcgaatgtgc
      361 tcggccttgg ttccgtgctc ctgtccggcg atcattccga tatagccgtc catcttattc
      421 tctttccatt cgcgggtcag agcgtccagc ggagtttggg aaacaacaat catgtcggcc
      481 tgccgggatc cttgacgcag cacatccatt acaaatggaa acggcgatat tccggaaacc
      541 atatcttcca cgcgggcgtt cacttctttg ctccactcca gcgcaatttt cagttcttcg
      601 cttccggtct ttttacactc cagctccagc gccggattgc ccagcttggt ttcccgccgg
      661 acccactcac gaagttcgtt gaattcagga actttaaaac cgcgcacccg gatatcttcc
      721 cgctctttca gcaggtcgca ggcataaagt accgcaaaaa accggttaca tccgcgcgtt
      781 ttgctgtaca ggtttacaaa atcccagact tcccgggccg ctttgctgac cggttgcagt
      841 ttgagatgtt taataaatgc cggacagaaa cactctttat gtttcaactc catcgtatca
      901 aacacacaac cgtcactgtc gaagccgata aaaaagtttt tatttttcgg catttccaaa
      961 agctgtttca cataattcat gatttctttt cctttacata acctcaaatg atccgcctcc
     1021 gtccgacctt gcgcccggat cttgcgatac accgggatcc tgaccggcgg aagcagtatt
     1081 ttcattagag gaaagcataa tttgacgcca aaacggatca acagatgaag ccggagaagt
     1141 ttatccgtaa actgtcgctg aaaatttgca gcacgcgtcg gaaaatcccc gcgtaaaagc
     1201 gggacatgaa tgacgccgcc atccgggttg ctttagcggg acggaggaaa tctggataat
     1261 ctgcggtatg gcagataatg cagaccggaa acggcacctg tccggccggt ttttgaatcg
     1321 ctaacaaatt gcatttccac ctccggatac tatagcttct gcgggtattt taaaatacgg
     1381 attacccggt gggtcgttaa ccgagccggt tttatgcgtc taaacgaagg agttcctgtg
     1441 aagaaactgc tgatcttggg tctgttgctg tctgtaggtt tgaacgcaaa ggcggcgctt
     1501 ccgccggaaa ttgaaaaccc ggaatgtctt ggggtgaata agctgccgta ccatgcgacg
     1561 ctgatgccgt atgcgacgct acagcaggcg ctggccggca agcggctgga gtcgcccttc
     1621 tgccagctgc tcaacggcac ctggaagttc aactgggtca agcaccccga cgagcggccg
     1681 ctgacgtttt ccgagccgtc attcgatgtg agcggatgga aggatattac ggttccgtcc
     1741 tgctggcagg tgctgggcta cggcacgccg tattaccgga actccggcta catcctcaaa
     1801 atggattttc cgcgcgtgat gaccgagccg ccgaaggatt ttacggcgta cctcgagcgc
     1861 aatcccgtcg gcagctaccg ccgcgacttt gatctgccgg ccgactggtc gggccgccgc
     1921 atctttctgg ccttcagcgg cgtggacagc gcgttctttg tctggatcaa cggccagaag
     1981 gtcggctaca gcgtcaacag ccgcaacacg gcggagtttg atgtgacgcc gttcgtcaag
     2041 ccgggccgca acatgatcgc ggtggaggtc taccgcttca gttccggcac ctggttcgaa
     2101 gaccaggata tgtggcggct ctcgggcatc ttccgcgact gctacctctg gagctcgccg
     2161 caggtgcaca tccgcgactt cttcgcgctg ccggacctcg acgcccagta tcaggatgcc
     2221 acgctggcgg tgacggccaa ggtgaagaat tacgccgatc aggcggcgga ggcccggacg
     2281 ctgacggccg cggtgtatga tgcagccggc aagatcgtcg cgcaggcggc gggcgaagtg
     2341 cccgcgctgg cggcgggcgc agaagccccg gtcgcgatgc agctgaaggt cgccaatccg
     2401 gcgaaatgga cggcggaaac gcccaacctc tacacgacgg tgctgacgct cggcaatcag
     2461 accgagattc tgtcgacccg caccggcttc cgcaaggttg agatcaagaa ccgcgtctat
     2521 cagatcaacg gcgtgccggt aaaacttaaa ggggtgaacc gccacgagat ggatccgaac
     2581 accggccaca cgatcagcga agccagcatg atccgcgaca tcgagctgat gaagcagggg
     2641 aactgcaact tcgtgcgcac cagtcactac accgacgatc tgcgctggta cgagctgtgc
     2701 gacgagtacg gtctgtatgt ctgcgccgag gcgaatgtgg aaacgcacgg gtatggattc
     2761 cgggaagaat cgctctcccg gcgcaaagac gccgaagcgg cgcatgtcga ccgcaatatc
     2821 gccaacgtgg agaacctgaa aaattatgcc tccgtcgtga tgtggtcgct gggcaacgaa
     2881 tcgggcccgg gacccagctt cctgtcggca ctcaaggcgg tcaaggcgat cgacacctcg
     2941 cgcccgaccc attatgaagg cttcgcgatc ggaccggcca acccggccga cgtggattcg
     3001 cggatgtatc cgacgattgc cgagaccgaa aaagcggcgc tcaatccgag ctataccaag
     3061 ccgtttttcc tctgcgaata cgcccatgcc atgaacaact cgatgggcgc gctgggggaa
     3121 tacaacgacg tctttgataa atatccgacg atcatgggcg gtgcgatctg ggagtggcag
     3181 gaccagggtc tgtggaaccg gcgcgatacc aaccgcgtgt ttctggccta cggcggcggc
     3241 ttcggcgacg tgccgaacaa taactatttc attcataaag gggtgatttt cgccgaccgc
     3301 tcgcccaagc cgcactatcc cgaaatgaag aaggtgtatc agtgtatcgg gatcggcgaa
     3361 gacgatctcg ccgccggcaa agtgaaaatc cgcaacaagt actccttcct gccgctcgac
     3421 cgcttcaatg ccgagtggac ggtgacgggc gacggccagg aaatcgcccg cggtgcgctg
     3481 ccgaaactgc cgattgctcc cggtacggag cagaccgtca cgctgccgct gccggtgatt
     3541 tccgcgaagc cgggcgttga gtatttcctg cgcctctcct tcaagctggc gcgcgatgag
     3601 cagtgggcca aagccggaca cgaaatcgcc tggcagcagt tcaaactgcc ggtcgctgcg
     3661 ccggctccgg cggcggatcc ggccgcgctg aagccggtgg ctctgcagga caagggcgag
     3721 gaactcgccg tcaccggcgc cggcttcgct gtccggttcg acaagacgac cggcgccatc
     3781 cgggcgatgg agcgcgacgg tgtcaacctg ctggctgcca atggcggccc ggcgctgcac
     3841 ctctggcgcg cggcgcatca gattgatgac aagtgggcgg tcgccgactg gcagcgctac
     3901 ggcctgcaga aagcctcggt gaagctggtg agcttctctg cgcagcaggt cgctccgtcg
     3961 gcggtgcgcg tgatcgtttc gactgaaatg cagggcgcgg cgaactttat ggtcagccac
     4021 agtgcggcct acaccattta cggcgacggc tcgattgcgg tggataacag cgtggtgccg
     4081 cagggcaaag tgattgcgct ggcgcgaatg ggcgtgcggc tgcagctcaa tcagcagctg
     4141 gatcagttca cctatttcgg gcgcggcccg atggaaaact atgccgaccg caagcgcggc
     4201 tccgacgtgg gtctttattc cagcagcgtg cgcgagaaca tgacgcctta ttccaagccg
     4261 atggaatgcg gcaaccatga agatgtgcgc tgggcggcgg tcagcggcaa aggactgccc
     4321 gctctgctgg cgcaggccga gtccggcgaa gtgctgcagg ccagtgcgct gccctatacc
     4381 gacgaacaga tggatcccgc cgcctacagc gtcgatctgc cgccgagcaa ggcgagcgtc
     4441 ctcgtgctgg cggtcaagac caccggcgtg ggctcgggca gctgcggtcc ccgcccggcg
     4501 gcggattgcg tggtcatgtc caccccgatc tcgttcagct atgtgctgcg gatgcttccg
     4561 gcgggcgctg cgctgccgca ggccggccgc cagccggctc cggcggaccg ggtcaagccg
     4621 gttctggtgg agcaggcgcc cagcggcgat gtgacgctga cctgcgcgac ggcgggcgcg
     4681 aagatggagt atgcggtgga cggtgcggcc tggcagcctt acaccgcacc gtttgcggtc
     4741 acccatgcgg ctaagatctc cgtccgcgcg acggcggaaa agctgacgcc gtacgaagcg
     4801 cagatttccc tgaaagcgct ggattaccgg cgcggctgga ccctcaccgc cagcagcttc
     4861 cagcgcgatc tcggcacgac ctcgtatgcc gtcgacggaa atccggagac gagctggtct
     4921 tcgatctggt cgagaggtcc gatgccgtat ccgcactggc tgtcggtgga tttcgggcag
     4981 ccgctcacgc tgagcgaggt ctcctgcctg ccgccgcaag gctcccgcaa cgttcagcgc
     5041 atcaaagact atgagctcta tctgagtaac gacggcaaga actggggctc tccggcggcc
     5101 aagggctcgt ttaaaaatac cggcgaccgc caggtgatcc gtctggccgc cccggcgacc
     5161 tgccgctatc tcaagctggt tgcgttgagc ggaatggacg ggcggatcat ggcctccatc
     5221 gccgagctgg atgtgcagat cgtggaatcg aaataagaga accggccggg ctgcttatcg
     5281 agaagcggcc cggttccggc agatgaaaat ccggtagggc aggtaggcgg taaagccggt
     5341 tgcgtccggc tggaacaggt ggccgcaggc gggatgtgcg gcgcagatgc ggtcgcggat
     5401 ttcaagccgg tcctgtgccg acagcgcata gcgcaggctc agatcttcgg cggtcagcgc
     5461 cagcgccgga gccggcagga tcgcttcaac gctccagccg gtctgctgca gcagttcggt
     5521 cagccggttt tctgccggat accacttctc cgtgcgcatt ctggcgtaga gctcgtttaa
     5581 acaatcggcc tcttcctggg agctgaagca ggcggtctgg tgaacgaagt attcatccgg
     5641 cccggtctgc cggcggatgt gctccagcgc gccggcgagc gcctcgcgcc ccagatagtg
     5701 cagcacggag cgcatcatat acagcagcgg cccgccgtcc gggttcaaat ccgcccggcg
     5761 gaacgtttcg atcgagccct gatgcgtcgt caccccggcg gcctgcgcgg ttttgagctg
     5821 ttcggacgaa ccgtccatcg tcaccagccg gaacggcagc ggcgcgaact gttccgtcag
     5881 cagccggctc agcgtgccgg ttccgccgcc cagatccacc accgtcgcgg cgccggagag
     5941 tccggcgcag cggatcacct gctccagcaa gggccgggcg acgtccgcgt tggaaaaata
     6001 tccgtcgtga aacgtgcccc agcggtttcc gtggacgatc tgttcgcgct caatttcccg
     6061 gttccggctc ataggattac agcagttgga ttttgaagga gacggcatgc cggctgggcc
     6121 aggctttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngct ggcttcggct
     6241 tgcacaaaca tcgaggggcc ttcgccgtaa accgtccagg gccgcgtgcc gaaaatcgcc
     6301 tcgccgttga ccggcatcca ggcggcgagc tcttccagca gcgcctcttc gtccgcgtcg
     6361 agggtgccgt tgccgcgcac cggaatgctc aacagcagat tgccgttttt gctgacgatg
     6421 tcggcgagca tatggatgac ctccgccggc ttcttgtact gatggttttc aaatatggaa
     6481 tcgcggtaat gccatttgcc gatgcaggtg tcggtctgcc agaccagcgg atcgatgcgg
     6541 tccgatttgc cgcgctcgat atccagcacc aggcacttcc gctgcagggc gttgagcttt
     6601 ttgccgttca tgacggcttc gttgcggccc gcatgagact gcatgctgac gttgtataga
     6661 tgcgcggcga tgcccaggcc gtaggcggcg ccctcttttt gcaacggcat cccggtgtcg
     6721 tcgaaataga gcagatccgg cttataggaa tcaatcaggt cgatcacgcg gttatagaat
     6781 ttatcgcagt aggccttgct cggcggttcg cccggaatgc ggttggtgtt gttgcggtcc
     6841 gccggatcgc tgctgggttt gtgccgctgg gcgtagaggt cctgcgggtc gaggccttcc
     6901 caccacttgc ccttgccgtc ggcggcggtg agattgccgt catacggcac gccggccttc
     6961 ggtccgctct tgtcgctcag ctgcgacacc tcataccact cccaggtccg cgccgcgtga
     7021 atgctcacgc cgaaccgcag cccggccttg cgggcggact gttcccacag gccgaccaga
     7081 tctttcttcg ggccgacgtt cacggaattc cacggctggt acttcgaatt ccagttgtca
     7141 aaattatcgt ggtggttgcc catcatcaca aaatactggg cgcccgcccg cttatagaga
     7201 ccgatcagcc gttccggatc aaactgatcc gccttccaga cgttgcagac atccttgaag
     7261 ccgaactccg acgggtgccc gtacttttcg aggtggccct tatacgaggg gtgtccttcg
     7321 acatacatat tgcgcgcgtc gcccttgccc ggctcgcact gcggtcccca gtgggcccag
     7381 atgccgaact tggcatcctt aaaccagtcg gggcactgat aattctgcag cgactcccac
     7441 gtcggttgaa atgctccgtt cgcggtctct tctgtccggg ctgcggttcc ggccagcagc
     7501 atccagctca ttgccataaa cagctttttc ataattctcg gctcctttga ttggtttcaa
     7561 aacgtcagga cttttcactt tattggaagc agaaacaagc cggaagactg agaacggctg
     7621 ttagtttgga gttgctcgcc gctctgcgcg cggtagtatg tcgagtataa cgatgttata
     7681 aaatgtgccg gagtcgaaag atatgggatg actcaggctg aagaaataaa ggacgatcaa
     7741 tgaaaaacct gaggaccgca gttatcggca gttcctgtgc tttggccatg agtctgtttg
     7801 cagaagaaca gaaaccgaac attattctga tgtacgtgga tgatctggat tttgatcaga
     7861 tctctgccta tgccgactcg gaatttccgt gctatacagg cgcaaaaaac accggaaatc
     7921 tgaaggagct ttcgccggtt acggctaatg aaaacgggca gttccttgtt cagggcgaga
     7981 tgtattatta taaaaacccg accatgctga ccccgaacat tgaccggctg gccgccggcg
     8041 gcatgaaatt taaccggttc tatgtcacct cttcaatctg tactccaagc cgctattcca
     8101 tgctgaccgg acggtatgca tcgcgctcgc cgcagctgct gcgggagact cctccgggaa
     8161 caactccgct gatcggctgg aactccttta tggatccgga tgaaagcaat ctggcaagga
     8221 gtttgaaatc tgcaggctat gtaaccggcc tggttgggaa gtggcatctg agttcatatg
     8281 aattggaagg gattgatttc ggcgccgggt ttgacagcta ccggacgcgg caggaaaacc
     8341 gcaagcaggt gctcccgcat cagatggtgg gcacctattt tcctctgact gccgactatg
     8401 gcgatcctgc cgtgcagacg gaagtgaaac gcatctatga aatcatgagc aaaaaagtgc
     8461 tgacgacgtc cgggtttgat gtggtagacc ggctctactt tgcgaattat ggcgaactgc
     8521 cgattcccct gcacatgaag gtgcacaacc tggaatggga gacggaagga gcgctcgatt
     8581 tcattgacgc caacaaagac cgcccttttt tcctctactt cgcgattaca ctgccgcatg
     8641 gatcctatac cgaagactgg atgtcaaaag actggcgcgc tacgcccgcc ggaatgttgt
     8701 cctcgcctcc gcgaggcatg ccttcgcgcg acagtgtccg cgcacgggtt gaggctgccg
     8761 gtctgccgcc tcaaaatgca atggctacat ggattgacga cagtctgggt gccattctca
     8821 acaagcttga tgaagcaaag ctgtccggga acacaatcgt tctgtttatt tccgacaacc
     8881 agtcccgcgg gaagctgact gcttatgagg ggcatcgcgt acccgccatt attctctggc
     8941 cgcgtcacac gcctgccggg tcggttgagg aacggattgt tgcgaacatc gacatccgtc
     9001 ccaccttgct ggagattgca ggggtccgtc cggcagacaa tgaaactgtt gacgggaaaa
     9061 gtctggtttc tattctggaa ggaaagattc ctccggcatg gcgcgacagt ctttttctgg
     9121 agacagctta cagccgcgct gtgataacca aagattggaa atatatcgcg aaccgtcctc
     9181 cgccggaagt tgtcgctaaa atggaggagg accgccttgc ggccgaaaag gaagggcgcc
     9241 ggcgtcacgt cgactggtca ggacgtgcta cggatccctc cagcgggctg ggagtccgct
     9301 ttaatgccga tcaggacttt ccgaactact ttgattccga ccagctttac aacctgaaag
     9361 acgatgtgtt tgaacagcgc aatctgttca gcaatcctga gtataaagcg accgcatcgg
     9421 agctgcatac gctccttggg cagtatctga aaaatatgcc gcgcggcttt ggcgagtttc
     9481 gctgaatcgg atcctggcgt tcagtccgcc tacatctgtc gtcgtactgt ctgctggaag
     9541 ctctccggcg ggatgccgta ggcgcgtttg aaaacccgcg agaaatgata ggggtcggaa
     9601 tatccgcttt cctgtgcaat ctgcttgatg agcattgtcc gtcctgacag gagttcggct
     9661 gcacggttca ttttgagccg ggtcagcagc tgcagcggcg tttcgctggc atagcgctgg
     9721 aaaagacggc acaggtaggc tttgtcgagc cggcaggcgg ccgccgcctg ttcaatgttt
     9781 ttgagccggc ggaagttttg ttcgaggagc ccgcggcagc gcagataggt ctgccaggcc
     9841 ggcgaaagga cttcctggtg cggcatgctg ctgtcgtcga tgtgcaaaat cagttgttta
     9901 aggagcagga ggcagagcgc tttctgatga cggctgtcgg ttcgtccggt ctgcagcaga
     9961 ttttcgaata cactaaggat tttgatgggg tgcgtggcgt agagcggacg ttgaaaaaaa
    10021 cgggtgcgct cgaccagcgt cttcagttcc tgcccggtga agtcgatgaa gtgtttgacg
    10081 agcggggcct gcggatcggt tttgatccgg tgggggatgc cgggtccgta gaaaaaaacg
    10141 gcgcccggct gaagcgggta ggtttcttcc tgcagggtaa gaatgccttt gccggcagag
    10201 acgaattcaa ttgagcagaa tttgaatccg tcacgatcga cctgatagtc gggggcgcac
    10261 tgttctctgc ctccgcacac gatggcggct ttgacatctt ttccggggga caggttgaga
    10321 tagtagtagt cgccggctga aacctgcgtg gagataaagg agggttcttt ttccatgggc
    10381 cgcactttta tcagggcggg gttggcaggt caataatatc catttaaatg tcaagagtgt
    10441 ctatttccgt ttcgtccggt tggtgtgtat gatgcggctt ttcctgagta cttaaacata
    10501 ggagcgtggt catgacagat gtcaaaaaaa caaagttcac cgttgccaaa gacgcagtgg
    10561 atcccaattc cattccgaaa aaaaagctga ataacggtac atcaatgccg gcgatcggca
    10621 tgggtacttt cggttccgac cgctttaccc cggtgcaggt tgcgaatgcg gtaatcggcg
    10681 ccgccgaata cggacagcgc gcctttgact gcgcgtcggt ctacggcaac gaaaaagaaa
    10741 tcggcgcctc gctgaaagtc attatggacg gcggcgttcc gcgcgaagaa ctctacatca
    10801 cctccaaagt ctggaatgac atgcacggcg aaggcaatgt catcaaatcc tgcaagcagt
    10861 ctctcaaaga tctcgggctg gactatcttg acctctatct ggtgcactgg ccgttcccga
    10921 acttccaccc tatcggctgc accgtggatt cccgcagccc ggacgccaag ccgtacattc
    10981 atgaaaacta catgaagacc tgggcgcaga tggaacagct ggtcgagatg gggcttgtca
    11041 agagcatcgg tacgtccaac atgaccatcg ccaagatgaa acttctgctg cgcgactgca
    11101 aaatcaaacc ggtcgtcaac gaaatggagc tgcacccgca cttccagcag ccggaactgt
    11161 tcgacttcat ggtcgccaac ggcatccagc ccatcggctt ctgcccgatc ggttccccga
    11221 cccgtccgga ccgcgacaaa accgcgaccg atactgtgga tatcgaagac ccggtaatta
    11281 agaaaattgc cgcgcgtctg ggtgtacacc cggccgtcgt ttgcatcaag tgggccgtac
    11341 agcgcggtca ggttccgatt ccgttctccg tattcgaacc cgaataccgc agcaacctgc
    11401 gttgcgcgct gccggaatat atgaccgtca ctggcgccga aatggccgag ctggccaaga
    11461 tcgacaagaa ctgccgcctg attaaagggc aggtgttcct gtggaaagac ggccagacct
    11521 gggaagatct ttgggatctc gacggaacca ttgcgaaata agttgaacag ggaacccggc
    11581 acacgccggg ttcctttttg tatacaggcg ggattagaaa acaggagaag acgtatgcat
    11641 gatacagatc aaaaagtgcc cgtggtgtcg aaagcgctgc tgggctcatt tattcttgta
    11701 accagtctgt ttgccttgtg gggttttgca aacgacatta ccaatccgct ggtgcgcgct
    11761 ttcaaggaag tgtttcccgg cctgaccaat gcccagagca gctgggtgca gggcgccttt
    11821 tacggcggtt atgccaccat ggcgattccg gctgcactgt ttattcgccg gttctcatat
    11881 aaagcaggga ttctgctcgg gctggcgctt tatgcgaccg gtgcgctgct cttcattccg
    11941 gctgcagtga ctaaacaatt cgtctggttt ccgatttcgc tgtatgtgct gaccttcggg
    12001 ctggccttcc tcgaaacaac tgccaatccc tatatccttg ctttgggtga cgagaaaacc
    12061 gcgacccgcc gtctaaatct ggctcaggct ttcaatccga tcggttctct catcggtatg
    12121 tttgtggcca gcaaatttat tctggcgaaa ctgtgggtca ctgatttccg caacgcggaa
    12181 atggctgccc atcccgaata taatgacttg ctgccgagcg tagttgacgc gaaaattgcg
    12241 gctgcgcggg aagcttttgc cgccgccaat ccggcccagt ttatcggcaa ccagacgcac
    12301 gacttgaatg ttattcgcaa tccgtatgcg gcgatcggta ttgtcgttct gattttcttc
    12361 gtcatgtttc tcgttaagaa aatgcctgtg ctgaaagatg atggtggttc catccatcct
    12421 tgggatacag ctaaacagct gttcagcatt ccgcgctggt ggtccggcgt aatcgcccag
    12481 atgttttatg ttgccgcgca gatcacctgc tggacctatg tcatccacta cgccatgacc
    12541 aacgtcggca tgagcatgga tgttgcgcag aactacaaca tcgccgcgat ggcgctgttc
    12601 ctgatcagcc gcttcatctg cactttcctg ctgaaatact tcagcccggg acacctgctg
    12661 atgacgctct ccgtggtggc gatcggcctg atgctcggcg taatcttcct tcagaactac
    12721 accggcctgc tctgcctgat cggcgtctcc ggctgcatgt cgctgatgtt cccgaccatt
    12781 tacggtattg cgctcgacgg catggggcag gaagccaagc tggcctcggc cggtctggtg
    12841 ttcgccatcg tcggcggcgc ctggatgccg ctggtgatgg gcggcattat cgaccatccg
    12901 gactttgatc tgggctttgt caagctgacc agcgtcagca tgtcgttcgt tctgccgctg
    12961 atcagttttg tggtgacggc gctgtacggc tatctgacgt tcaagatctt ccgccccgcc
    13021 tcggagctca cccgtaaata acttacgagg attcttatgt taaaaggaat cgacccgatc
    13081 atttgtcccg agctgttgaa aatcatggcc gaaatgggcc acggcgatga actcattctc
    13141 gccgatgcgc atttcccggg aaacaccttc ggcaagaagg tgcttcgcgg ggacggcctg
    13201 cagattccgg ctctgctgga aggtatcatc ccgctgttcg aactcgacag ctatgccgac
    13261 ccgctgatca tgatggcggc cgtggaaggg gataagctcg acccgaaagt cgagaaatcc
    13321 tatatggccg ctatccgcaa acacgcgccg gaagccccgg cgccggtgcg catcggccgg
    13381 ttcgatttct atgaccgcgc caagaacgcc ttcgcggtgg tcatgaccgg aacgaccgcg
    13441 aagtacggca acattattct gaaaaaagga gttactccgc tggcctaaag cggagtattg
    13501 gcggctatca aaacactgta tttcatgcgt cacgcccgga gtgaggcgaa cctgaaggat
    13561 attctggctt cgcggcttga ttttcccctg acggaagaag ggcgccggga tgccgaagcg
    13621 attgcccgcg aattcgcggc agtcgcttct gttgaccgga tcgtttcatc gccgttgatc
    13681 cgcgcccggc agacggcccg gccgtttgcc gaggcctgcg ggctgacggt gcagaccgac
    13741 gagcggctga cggaacagca tctgggcata ttctccgggc tgacctatgc ggaactcgat
    13801 acgcggcccg attacatgca tgaccgcgcc cagcgctgga cgtgggttcc ggctggcggc
    13861 ggagagtcct atcagatgat cgccgaccgg ttgcggccgt tctttctcga tctggaatcg
    13921 atgaccggct cgtcgatttt gtttgtcact catgccgtca cgatgcggct gatccgcgcg
    13981 acgctggagc agacgctgcc ggagtatccg cagacgattg cgaaaaacgg cgaaatctgg
    14041 aaggtgagct atacgcagta cggcgcgcca caccgggtgg aatcaatttt tctgggcggc
    14101 agcgccgccg ctgtttctcg ggcttaacgt agaggagtaa atacatggct gaatatattc
    14161 ttagcatcga tctgggaacc acgagctgca aggccgtgct gttcgatacc gatttcaaca
    14221 ttgtgaccac cgccagacgg gagtacaaaa ccagttatcc gcacgccggc tgggccgaac
    14281 agcccgctta ccagtggtgg gaagcgttgc gcgaaaacac gaaggcggtg ctgaagcaga
    14341 gcgcggtcga tccggctaaa atcatcgcgg tcggtattga tgcgttctcg actactgttc
    14401 tgccggtgga taaagccggc gacccgctgc gtcccggtct gatctggatg gatcgccgct
    14461 ccggaaaaca gtccgactgg attgccaaaa acctcggcaa agagctgtgg gaaatcaacg
    14521 gcaacgtcag cgacgccgga aacatcgcgc cgaaaatcat gtggatcaaa gagaacgagc
    14581 cggaagtcta caaaaagacc cacatgttcc tgcacgcaaa cggctatctc gtttactgcc
    14641 tgaccggcaa atactcgatg gatatttccg aagccggact ctctcagctt tgcaacaccg
    14701 caaccggaca gtattcggat gagctcatca atgcctgcgg cattgaccgc gccaagctgc
    14761 cgccgatttt caactgcacg gatgtcgtcg gtaaagtcac cgccgaagct gccgcgaaaa
    14821 ccgggctggt tgccggtacg ccggtcatcg ccggctcgat ggataacgtc gccgccggac
    14881 tcggcgcggg cgtttcaaaa ggcggcgagg tctttatttc gggcggaacc gtcaccacca
    14941 acaacgtttg tttaagcgtg ccgaaataca accgcaacct gcacatttat ccgcacattg
    15001 tccccggcac atggattacc gccggcggcg tagacttcgg cggcgccggg ctcaaatggt
    15061 tcaaagagct gcttgaagtt gaaaattacg ccgagataga tgcgctggcc gatacatcag
    15121 tctgtgcgaa aagctcggtc atttttcttc cctacatggt tggccagcgc tgcccgatct
    15181 ggaatgacca caccaccggc gtattgatcg gcctgaagcc gaccaccaac cgtcaggatc
    15241 tggtacgcgc cttcatggaa ggcaccacct acggctcgcg ccatgttctt agcctggtgc
    15301 aggatgaagg cgtcgccatt gaaaacgtca aaatcaccgg cggcagtgcc aactctgcgg
    15361 cttgggtaca gattttttcg gacgtcatcg gcaagccgat cgacatcccc ggtgcggtgg
    15421 atctgccgcc gctcggcgtg gcgattgccg ccgcttacgg cgtcggtgct attaaaacct
    15481 ttgatgaagg catcgctaaa attgcgattc gcgaaagttt tgtaccggat gaggagaatc
    15541 acgcctacta caccgaaatg tacggcgtct tccgctcgct ctacgaaaac atcaaaggcg
    15601 aatacgaccg gctcgccgcc attgaacgga aatttaaaaa gtaacggagg aacccctgtg
    15661 agagctgcat tacgcaaagg acagaaaaaa gttgaagtga ttgaaatggc caaaccggcc
    15721 ccgctggcgg atgaagtgct ggtggcggtt aaatcctgcg gaatctgcgg atccgacatc
    15781 gtccggattc aggaagaaaa tccgcgctgg gacgaagtcg ttctcggcca cgaattcgcc
    15841 ggacagatcg tcgaagtcgg cgcgaacgtc aaggagtgga aagccggcga ccgcgtatcc
    15901 gctgcgccgc tcatgccgtg tcatcagtgc gcgcactgcg cccagggcaa cttctcgctc
    15961 tgcaaaggct acagtttcat cggctcccgc gtgcagggcg cgttcggcga attcctgcgc
    16021 gtaccggccc gcaacctcgt cgcgatcggc gacctgacct atgaccaggc ggccttcatt
    16081 gagccgatca ccgtctgtct gcacccgatt ctgcgcctcg gcaacctgct cggcaaagat
    16141 gtcgtcgtca ccggtgccgg aaccatcggc ctgctggcga ttcagatttt caaagccatg
    16201 ggctgccgca acatcgtggc ttccgacatt tcggaaggca aactcgagat ggcgaagagc
    16261 atgggcgcga acattctgtg caacccgatc aaagaatcgc tcgaaaacgt ctgcgcccgc
    16321 gaactcaaag acggcgcgca cgtcgtgttc gaatcgtccg gcgccggtcc ggccaagatt
    16381 tccgcgatgg cgattgcccg cggcggcgga accgtgctgc tggtcggtac ggcgcacgca
    16441 ccgatcacct tcaccggtcc gcagttcgag cagatcagcc gcaaagaact ccatgtcgtc
    16501 ggttcgtgga tgaattattc cgcgccgttc cccggcaccg agtggtccac cgccgcgtgg
    16561 atgatgtctg ccggtctgat caaggtggat gcgctgcaga cgcacacctt cccgattgaa
    16621 aaaattcagc aggcctacga cgtcatttac accaacaaag agctgtgggc caaggtcatg
    16681 atccacttct aaccgaaaga caatccgatg agcgctatcg aaaaatttgt acagattctc
    16741 caagacaacc gcgccggcaa agccaccggc atctactcca tctgctccgc gcaggaaacc
    16801 gttctgcagg catccatgct gcaggcgaaa aaagacggct cgatcctgct gatcgagtcc
    16861 acctcgaatc aggtggacca gttcggcggc tacaccggca tgaagccggc ggacttcgtc
    16921 gcctacgtgc gcggcatcgc cgcttccgtc ggcttcgacg cccaaaacct tctgctcggc
    16981 ggcgaccacc tcggtcccaa cgcctggcag aaagagccgg ctgcgtcggc gatggctaaa
    17041 gcgcacgacc tgatccgcga atatgtcaaa gccggttacc agaaaatcca tctggacgct
    17101 tcgatgtttc tcgccgacga ccagggcgac cgccataaag cgctcgccaa cgaaatcgtc
    17161 gcggagcgcg ccgccgcgct ttgcaaaacc gctgaagaaa cccacgccaa atactgcgcc
    17221 ggacagccga agccggttta catcatcggc accgaagtcc cgattcccgg cggcgcgcag
    17281 gaagccgaag aaaccgtcac gccgacttcc ccggcggatg ccgtgcagac gatcgaagtc
    17341 acccgcgccg ccttcgaaaa ggccggactg accgaagcct ggaaccgcgt gtgcggcgtt
    17401 gtcgtacagc ccggagtgga attcggcgac gaccaggttt tccactacca gcgcagcaag
    17461 gcccgcggcc tcagcgaaaa aatcatgcag ttcgatcgtt tcgtctacga agcgcactcc
    17521 accgactacc agtccgaaac cggactgacg cagctggttg aagatcattt ctgcattctc
    17581 aaagtcggcc cgtgggtcac cttcggctac cgcgaagcgc tgtttgcgct cgcgcagatc
    17641 gaagaagaac tgctcgcgcc gaaaggcctg cagacctcgc gcctgcgcga agtcatcgac
    17701 gccgtcatgg tcgaagacac gaaatactgg gccaaatact atccgggcaa cgcgaccgag
    17761 caggcgtaca aacgcaagta cagcttctcc gaccgctcgc gctactactg gcccaacccg
    17821 cgcatcacgg cggccaaagc gcagctggtg gccaacctca aggcgcatcc ggtgccgctg
    17881 tctctgctca gccagtacat gccgggccag tatcgcgccg tcagcgaagg cctcatccgc
    17941 aacgacgtgg aagaaatcat catccaccgc acgcaggatg tgatcagcct ctacgcccgc
    18001 gcctgcaaca tggcgcgccg ctaaggagtg agcccgatga atcttgcgcg gatgctggac
    18061 gtgttggtga tttgtgttcc ggtgttcgcc gttctcgggc tgggcaaact gcttcagcgc
    18121 aatggccgtc tggatgacgg ccattgcaat tttatcaact ggctggtcta ttatttttcc
    18181 ctgccggcgc tgatcttcac gacggtggcc cgccagcggt tcgaagggtt tctcgatccg
    18241 gcgctgattc tgatgccgct gctggcgctg attctggtga ttgcggtgac catgctgatt
    18301 gccaagctga gccgctttaa aggcggattc gccgcggcgt ttgtcttcgg aacgttctgg
    18361 gcgaacgcca cctatctcgg tctgccgctc tgcaccaacg cgttcggcgc cgaggggctg
    18421 gccaaagcgg cggtctataa cgcctgcgtg gtgccgttct ttatcatctt cggctactcg
    18481 ctgctcggtt tctacggcgc gggttccggc gatttaaaaa tcggctcgcg gattcgcacg
    18541 gcgctgctga atccggtgct gctggcggcg gtggccggga ttctggtggc gctgtcggcg
    18601 gggctgttcc gctcggcgga tggcgcgctg aaccttcccg tgccggtgct ttcggccgcc
    18661 acgctgctcg gttcgttttt ccagctggtc ggctcgatgg ggctgccgct cggacttctc
    18721 tccattggcg cttcgctccg gtgggagcag gcgcgcggac atctggtcgc gctgagctgg
    18781 acagtcgggt gcaaactgat tctgctgccg ctgctgacgt tgctgctgat ccgccggttc
    18841 tgtccttccg ccgatgccgt atcgctgggt tcggcggtga ttctgtcggc gacgcccggc
    18901 gcggtggcct gttacatcat cagctgcaaa ttcggcatcg aaaaatcatt cgcggccgcg
    18961 ctgctggtgg tcggtacagc gctgagtgtg ctgaccattc cctgctgggt ctatctgctg
    19021 aagagcttct gagtgaaccg ctacgcacgt tccggcggca cccgcgccga cgaactgaaa
    19081 actattctga tctcccggat gtgatcgtgg gttctgcttt catgctctcc aatgggcgag
    19141 gaattcgaca ttgcctttgg gtcctttgag cggcgatgtc gccagcccca gccattcaag
    19201 cttgagcgtt tcagttccga atgtacggat tttggcaatt actgcttcgt gaatttccgg
    19261 atcggttatg acgccgcgcc ctttatccac ttcttctttg cccgcctcaa actgcggttt
    19321 gataagcgaa acaatttctc cgccgggttt cagcaagcct ttgacggcgg gcaggatatt
    19381 agtgagtgag ataaatgagg tatcaatgga agcgaagtct gccgcttcgg gcaggtccgc
    19441 aggagtcagg tagcgggcgt tcacgccttc catgaccgta acacgagggt cttcgcgcag
    19501 tttccagtgg agctggcctt tgcctacatc cacggcgtaa accttcgtcg cgccgtgctg
    19561 aagcatgcag tcggtgaagc ccccggtaga agaaccgatg tcgatgcaga ctttgccggc
    19621 cagatcgagt ttgaaggcct ccatcgcacc ttcgagtttc tctccgccgc ggctgacaaa
    19681 ccgttcgact tctttcagaa caatttcgcg gtccggcgac aacgtctggc ccggttttgg
    19741 agagggctgg ccgtcaacca gaacctgtcc ggcgacaatc agccgtcggg ccttttcgcg
    19801 gctttcggcg agtccttttt ccaccagcag ttgatcgagt ctgatttttt tcacgtgcgg
    19861 gagtgtaaat cctaagtcgg caatctacaa tcggcaatca tggcaggtct gtacattcat
    19921 attccgttct gtgagcgcaa gtgttcctac tgcgcatttt attcttttgc taaaccttgg
    19981 aaaaacgtgg ccggattttt ccaggcgctg ggaaaggagc tgaacagcct gccagctgat
    20041 ttttcgccgg agaccgtttt tatcggaggc ggaacgccaa cggcgctgga tttgaaaaca
    20101 ctgggatctt tccttgccat gctaaggaag ttcagtccgg tagaattctc gtgcgaagcc
    20161 aatccgggaa ccgttgaccg gaagaagctg gagctgctca taaaaaacgg cgtgaaccgg
    20221 ctttccatcg gcgtgcagtc gtttaattcc aaatgtctgg aaatgctggg gcggattcat
    20281 actgcggaag aggcggaggc cgctttccgg ctggcgcaga cggcaggttt taaaaatatc
    20341 ggactggatc tgatgttcgg agttccgggt cagacaatcc aaatgctgga ggcggatatt
    20401 gaacgtgcgc tggcgctcga acctgagcat atttcaattt acaacctgat gtatgaggag
    20461 ggcactccgc tcgccgtccg cgatccggaa cggctggacg agggacttga gcgggaaatg
    20521 tacgatctga tccgccggca gctggcggca gccggcttta ttcattatga aatttcaaac
    20581 ttcgcgaagc ctggattcga gtgccggcat aatctgctct actggagcgg tggagaatac
    20641 atcggactgg gtccggcggc gcattcccac tggcggggaa tgcgccgggc gaatgtttct
    20701 gatgttgagg attactgcgc aggcgggccg cggcgtgaat tcgaggagat cctcgatccg
    20761 gaggcgaagg aacgcgagac gctggtgatg gggctccggc gtttggcagg cgtcgaggtg
    20821 tctgttgatc tgtttgaaaa taagaagaaa acctttctgg cattggaaaa agaaggcctg
    20881 ctggtgatca gcgggcggca cgtccggctt tccgaagagg ctctgtttgt gagcgacgcg
    20941 gtcttttctg aattggtgtg atattgcgct tgccaatggg ccggccagtc cgtagattac
    21001 gccactcttt gagttaaatt tttgagtttg tgaggaagaa ttatggctaa atgtgcaatg
    21061 acaggtaagg gaacgatttc aggccgccgg attgtccgta aaggtttgag gaagtgtaag
    21121 ggcggtatcg gtctgcacgt gaccagcgcc acgaagcgca aattcaaagc gaatcttcag
    21181 cacgtccgga ttaaagatga aaacggcacc gtgcgtcgcg tttgggtttc aaccaaggcg
    21241 atccgcgacg gtctggtgac aaaagcctaa acgaaacgaa aagcctgttt tcggtaagcc
    21301 ggcccactgg gccggttttt ttgtgcaggc agatacgtaa cggaatgctg tctggcgggt
    21361 aggttctgaa tgatcgcgtc agaataaaaa aaacactctc cgcaaacgga gagtgtcttg
    21421 gacggaacca aaaagcaggg gttaccagct ggctttggtc atgccgggga tgagcccgtt
    21481 ggatgccatt tcgcgcaggg tgatgcgcga gaggccgaat tttttgtaga cggcgcgcgg
    21541 gcgaccggtc acacggcagc ggctttgtac gcgaatcggg ttggcgttgc gcggcagttt
    21601 gcgcaacttg tcgaatgcgg ccatgcggtc ttccatggaa gttttcgggt tgtgaatgat
    21661 ttccttaagc tccatgcgct tggcgaagtg cttctttgcc agcttcatac gcttttcatt
    21721 tttaacgata ctgctcgttt ttgccataaa tcacattcct caattggttc gtcgaagcgg
    21781 ggatgaaaca ggaaaaggct ccatggtgca acccttttct atgcaaaatt cgccggtcat
    21841 aggcagatcg gcttgttgtt ttaaattttg tttccgtggc tttttcaggc aggcttcatt
    21901 tcgatttttg gactttccgg gaggaggaat tgatgtgcgg agcagcctgt ccggcgaatc
    21961 tggaaaaatt gccggcggcg aagccgatta aaaagagacc cgcggcaacg ggggtgttta
    22021 tagaataggc ttccaatgag cgggcttttt gcttgaaaaa ggccctcggt ggcaaataca
    22081 ctcctcggcc cctgtaatca gagaggttga ctatgaaact agataaagtt ggacaggctg
    22141 aaatacatat cgatgaccgt accgtggtct gccccgttta tgagggctcc gaaggcgagc
    22201 gggcgattga tatcggcaaa ttgcgtaaag aaaccggcgc aatcacctac gacccgggct
    22261 atggcaatac cggctgctgc gccagcgata tcacgtttat tgacggtgaa aaaggcattc
    22321 tgcgctaccg cggttatgat gtcgcggatt tggcggagaa atgtgagttc atcgaagtgg
    22381 cctatctgct ggtccacggt tcgctgccga ctcatgcgca gcgggaaaac tatgcgaccc
    22441 ttctgaacag ccattctatg attcacgaag atatgcggga atttttcagc aattatcctg
    22501 agcattcgca tccgatggcg attctctccg ccatggcggt ttcgctttct tccttctatc
    22561 cagaactgcg cggcgacctc aaagaagacg tggatatgac ggtcacgcgg ctgatttcaa
    22621 agctgcgcac gatcgccgct ttttcttata agaagtccat cggcgagccg gccgtttatc
    22681 cgcggtacga tctgaagtac tgcgaaaatt ttctgaacat gatgttcagt tcgcccgttc
    22741 gcggcgagcc gatcgatccg gttatggccg atgcgctcaa taagctgctc attatccatg
    22801 ccgaccacga acagaactgc tccacttctg ttgtgcgcag tgtaggaagc tccggaatca
    22861 atctgtatgc atcgattgca gcaggtattt gcgcgctgtg gggaccgaag cacggcggtg
    22921 ccaatcagca tgtgattgaa atgctggaag aaatccaccg ggacggcggg gatgttgaca
    22981 aagtgatcga gcgcgccaag aaccctcgca gcccgttccg gctgatgggc tttggacatc
    23041 gcgtttataa atcctacgat ccgcgcgcca agattgctaa agagctgtgc aaaaaggtga
    23101 tcgcggcaac gcatacaaga gatcctttga tggatatcgc catggaactc gaggagaagg
    23161 cactggctga ctcctatttc caggagcgca atctctatcc caacgtggac ttttataccg
    23221 gactgaccta tcgcgaaatg ggtattccaa ccaatatgtt taccgtcatg ttcgccatcg
    23281 gccgcctgcc gggctggatt gcccaatggc tggagatgca aaacgacagc gatgcgcgca
    23341 ttgcccgtcc gcggcagatc tacaccggcc cgaacctgcg caaagttact cccatcgcca
    23401 aacgcaaata gtggattttc agaacgtgat accgaccgac cagcagccgg aaaccctccg
    23461 cgagaataaa caggaacttg aatttctaca ggccatcgcc cggcggctgc ctgaagatac
    23521 cgatgtattg cgcgcgttag ccgaccttta cacccggacc ggttcctatg ccgatggctt
    23581 gcgcatggat gaacgtctca gccgtctatg cgccgaagat ccgctggtct ggtacaacct
    23641 ggcgtgcagt ctggctcttt ccgaccgggc cgatgatgcg tttgacgcac tgaaccgtgc
    23701 ggtcgaactg ggctacaacg attacgaatg gatgaagaaa gatcccgatc ttgctgcctt
    23761 gcacggagat gcccgcttcg aatcgtttct cgagtggatt tacagcgcgt tcgagcagac
    23821 gccggactgc gacggactgt aagttttctt ccaggccggg aaaaacggtt gatccccgtt
    23881 ttcaatcgct gtagcttcag caaatcttat tcgcaggaga gtagtttatg aataaatcat
    23941 tgctttgggc tttgtttctg atcgcactgt gcgcgctgtt tttcattttt accggcggca
    24001 aaaccaccat ccggattttt gagctggatt acaccatgaa aacgcctatt gctttgctga
    24061 tcttcacggc gatcggaacg gccattggag tccttcttaa atgacggatg ccttctcccg
    24121 aagaggagtt tttataggcc ccgaagttaa ccctgcgcgc attgcgccgg acgcggaggt
    24181 ttatcccggc tgtcgcattc gcggcgcaaa aacttccatt ggacccggct ctgtacttgg
    24241 tgcggaagcg cccgttacaa ttgaaaactg ccagctcggc cgcgatgttc aattaaaggg
    24301 cggcttttgt tcgggcgcgg tttttctcga tggcgccggc atgggcagcg gcgcgcatgt
    24361 ccgcgaagga accatttttg aagaagaggc gaacgccgca cacacggtcg gtctcaagca
    24421 gacggttctg cttccgttcg taaccctcgg cagtctcatc aacttctgcg atatcctgat
    24481 ggcgggcggc accggccgaa aaaaccacag cgaagtcggc tcctcgtacg ttcatttcaa
    24541 cttcacgccg catcaggaca aggccaccgc ctcgctggtc ggcgatgtgc cgcgcggcgt
    24601 attgcttaat cagaagccga tatttctcgg cgggcagggc ggactggtcg gtccggcgcg
    24661 catagcgtac ggaagcgtcg ttgccgccgg cggcatctgc cgcgaagacg tggttgaaga
    24721 aaaccagctt cacattccgg ccgtaccggc ggagaaaacc gttccgttcg aaaccggcgt
    24781 ttacaaaaac attgaccgca tcgtcaaaaa caacctgatc tacatcggga atatctgcgc
    24841 gctcaaggcg tggtacgaaa acgtccgcgt ttttttcatc cgcgatgaat ttgaccgtgc
    24901 ctgccacgaa ggcgcgctgg ccaacctcga tctgattcgt aaggaacggg ttcaaaggct
    24961 ggagcaattg gtaggaaagg tcggagattt atccagaagt tggaaaaaga tttgtgtcag
    25021 gcttaaagag ccgccccgta ccggcgctga tgcgcagctg atgagtgaaa tcaagaaggc
    25081 agacaaggcg gattacatcg ccgcgataca gaatttgagt gaagacgcat gtgccggaac
    25141 gacggcgtgg cttcagggga ttgtggatga aacggcggtt ttggacggct gaccgaagaa
    25201 attgaacatg gcaaagcttt ccggaacaga cggtaatata tgacggcaga acaggtgccg
    25261 gctatcatgc gcgagaccgg cttgccgaag aagtgactgc agtatcaaaa aatctgagag
    25321 gggttcaata tgaccccgaa aacgattgaa gcgcggacca aaagaagctt gttgccgctg
    25381 cgctgaaaat ggccggcgag gggcagtgag aagaaagctt ttgcgggttt tgtccggctc
    25441 tgatagtttc tccgcctgaa tgaaacgaac gtttttaaaa ggttttatct ttctgacggc
    25501 ggcggtgctg tacggcacgg ccgccgcaag cagtctgttt ccgttctatg acaacctgac
    25561 gacggagcag gggtctttct tcgcggttcg tccgttctat tcgcataccg tcgtcaaaga
    25621 aggtcaaatc cgtgactgct tctggccgct ctattcgcgc aaggatttca aggatgagca
    25681 gagttcgcgc gcactgattt tctggttcac ccataaattt gatgcaaaag acgcaagccc
    25741 gcgtgatcgc aactggctgc tgccggttta ttttcaaggc cgggacgtaa acggcaaagg
    25801 atactttgcg ctgtttccgc tcggcggaac gattaaggaa ttcctcgggc gtgacgaaat
    25861 catgttcgta ctttttccgg tgtacggaca aagccggatt aatgacgtaa aaacgaccag
    25921 cgtattttgg ccgatctact cccacacgcg cggcgaagga attcaacgcg accgcgtttt
    25981 tccgatttac ggaaaatcgg tgctcgaggg gaaatatgac aagcgctttg tgctgtggcc
    26041 gttctggacg tcggcagaat atttttatcc cggcaatgcc ggaaaatcat ggatcctttt
    26101 tccggtttgc ggccggtcga agctggagca ggagaagacg ctttgggtca ttcctccttt
    26161 cttccggttc acgaaaggag aaaagcagga caggcttttc tgtccgtggc cttttattca
    26221 aaagaccgac agcaaaccgc acagcaagtt ttatatctgg ccgctttggg gtgaagaccg
    26281 gtacgccgcc ggtccgacgc agcgtacatt cctgctttgg ccgctgctct ggtcagagaa
    26341 aacgggcagc gacagcctgc tgagaaccca ccgcatggcg ctgccgtttt tttatcagga
    26401 ttacagcgtt cagcgcgaaa aaggtgtttc gccggaaaag cggacgaccg tttccaatta
    26461 ctggaaaatc tggccgctga tgagctggca gcgcgaaggc gacgtttcgc gtttccggat
    26521 gctggaactc tggccgctta aaaacagcgc gccggtcgaa cgcaactggg cacctctctg
    26581 gacgctgtat aaacggacag atatggaagg aatcatccgt aaggatattc tgtggtttgc
    26641 ctggcacagt gaaaaagagc cgtccgtcga gcgcaaggaa tggtctttac tgaaagggtt
    26701 tctggcgtat aaaagggatc acgaatcgca ggagctgcgt ctgctttatt tcatacattt
    26761 agggaatagc gaatgatcgc gccgtttaaa gaaatatatt ggtatctgga tctgccggta
    26821 cggtcgttcc gccgtctggg ccggatcatt ctggttgcct gtcacgatat gggcaacgcg
    26881 ctcatcatgc ttttttctgc gacgctttat atccgtacgc tttggaaacc gcgtgcacgc
    26941 cgcgaaacgg ccatgcagct ttatgtcacc ggcattaaaa gcctcggcgt cattactgtt
    27001 gtcgcctttt ttaccggcat gattctggcc ttgcagaccg gccttgaact gcgccgcttc
    27061 gggcaggaag tcaatatcgg cacagcagtc accatcgtta tgatccgcga aatgggcccg
    27121 ttcatgaccg gtctgattat cgcagccagc gtcggttcgg cgattgccgc acagctcggc
    27181 accatggcgg tttccgaaga aatttccgcg ctggaagtca tgtcgatcaa tcctaaccgg
    27241 tttctggtga tgccgaggct ggttgcgctg gctgtgatga tgccgattct gacggtttac
    27301 accaatatcc tcggcatcct cggcggcgga attgtcggtt cgacgcagct cggcgtggcg
    27361 tttcaggcct atctcgataa cgccacccgc tacgccgaga acaaagacct gtatgtcgga
    27421 cttttcaaag cgtttgtctt cggcatcatc attgccaccg tcgcctgcta tcaggggctg
    27481 agcactacgg aaggcgctgt cggtgtcggg cgcgccaccc ggcagaccgt tatcatttca
    27541 ttcctgatga tcctgattat cggctacatg atcacgaggc tgttttacat atgattattt
    27601 tcgacaaagt caccaagcgg ctcggcggaa aaaccgtcct caatgaggtc agttttgaag
    27661 tgccgaaggg tgaaacgttt gtgattgtcg ggctttccgg cgccggcaaa agcgttacgc
    27721 tcaaacatat ggtgcagctg atggttcctg acagcggtac ggtgcgcatc ggtgatgata
    27781 tcatcagtgc agcccgcggc accgaactgg aacgtatgcg ccgccgcttc ggtgtgctgt
    27841 tccagggcgc agcgcttctt cagtggctta ctgttttcga aaatgtggca ctgccgcttc
    27901 gcgagcatac gaagctgagc gaagaggaga ttgatcggac tgttcgcgaa aaactgcgtc
    27961 tgctcaatat ggaagacgca atggataaat ttcctgccga tctttccggc ggcatgcaga
    28021 aacgcgtcgg cctcgcccgc gccatcgtca tgaatccgga aatcattctc tatgacgaac
    28081 cgacgtccgg tcttgatccg gtgacatccc gcctgatcga taaactgatc gaaaatctgc
    28141 gcaaagaact tgggatcacc agtgtcgtcg taacccatga tttgcacagt gcgctggcaa
    28201 tcgctacccg catcatgatg attgatggcg gggaaattgt tgaaaacgcg acgccggccg
    28261 aatttattca atcaaccaac gaaaccgttc aacgtttcct cgagtcccag tatattaccc
    28321 gccggggtgc ctgggagaaa ggaatggagc aacatgtctg acaaatacaa caaagaactc
    28381 agcatcgaag tccttgtcgg cttctttatg tttatcattc tcatcgctct gggtgtgttc
    28441 accattatcc tgagccggca gaatttcctg cagaaaaaat acccgctggc ggtccgcttc
    28501 agtgaagtca gcggcctgcg tgacggcgat aatgtttttc tgcgcggtat gaaagtcggg
    28561 gttgtaaaaa cgacggcgct ggaagaccac catgtggttg ttcgtgccga tcttgaagtg
    28621 ccggtacagc tccgtgaagg ctataaagtg gaagtggttt cctcctccat gctcggcggg
    28681 aaacagctga aaatcgatga aggccccatg agcgcatcat tgcttccgga aaatgcgccc
    28741 attatcggca cggagccgac ggacattctc gaagagcttg gcgcggctgt ggccggtatc
    28801 cggcaaatga ccgataagat ttctgcgggc gaaggaactc tcggcaagct gatccatgac
    28861 gatgcggttt ataataacct gcaggaatct ctggccaatc tgcgtgatgt cagcgacaag
    28921 ctggccaaag ggcagggaac catcggcaag ctgattaatg atgaaacggt ttataccgac
    28981 ctgcaatcaa ccatggccag cctgaaggat gtcagcggcc ggctggcgaa cggggaagga
    29041 accctcggca aactgctgtc caaagacgag cagctttacc aggatctcag caccacgatg
    29101 gccaacctcc gcagcatcac ggataaagtc ggctcaggtg aaggaacgct cggcaagctg
    29161 gtcaacgacg atcagattta taccgaagcg cagaaactgc tcggtgaact gcgtgccgcg
    29221 attgacgata tgcgcgaaac gtcgccggtt accaccttca gcaccatctt cttcggcgcg
    29281 ttttaaaacg gcttgccggg gttgttgcgc cggacgattt cttttaaaaa gaaaacaagt
    29341 tctggaaatc ccggctgaga aatgatttcg ttcttccttc tgacaggagg aaggcgatga
    29401 acagttcggc cagcgaaaat gaaatttatc cggcttgcgg attgcaggtt gatcgtcctc
    29461 tcaaaatacg ggttcttcaa tacgcctcgc tggtgctggc tgtcgccgga ctgattctgc
    29521 tttatctgtt cgcggtaaac cgggatctgc cgtttgtccg gatcggcgac atcacgccgg
    29581 ccatgaactt cgcttatgtc cgcatctccg gcgaagttac gcgtgacgcc tacgtttttc
    29641 agtccggcgg catcgtattc gacttaaaag acggcagcgg cgaaatcgct gtcatgggcg
    29701 gaaaagcgca ggctcaggcg cttgaagctg ccggaaaact tccgcgacgc ggggaccgga
    29761 tcgaagtggc cggcagtctc agtgttgccg ccgatcagga accgcggctt cgtatccagt
    29821 cggcggatca gcttgttctc aaccgcaagc gggccgccgc cgcttcatct ccggcaccgc
    29881 gcatccggct ggctgatgtt accgccgcgc ataaaggaga gcagatatct gtttccggca
    29941 tcctcaaaat tattgatgtg cctggacccg gctcccgggc gccgtacact ctgacacttg
    30001 aagaaaacgg cgccgagctg gctgtggttt tctgggaaga tgttttccgg gatttggaag
    30061 acaggctgcc tctgcccggg aaacggctca gcgttcgcgg aggggtggat gtttataaag
    30121 atacggtgca gatcaaagtc cggtcggctg gcgatctgcg catcgcggag tcgggacaat
    30181 gatctttaca gcggtggcag cggtgctggc cggaaccgtt ctggcctgtt ttctctccat
    30241 cctgccgggg ctgcacattt acaacgtcat gggtctcgcg gcgctgtttc tctattccct
    30301 gcagggaagc ggtgttgccg ccatgccgga agtttacatt ccgttcatgc tcggacttgt
    30361 caccggctgg tcggtactca acaccattcc ttctgtcctg ctcggagccc cggatgaaag
    30421 cgcggtcttt accgtgctgc ccggccagaa atatctgatg accggtcgcg gctacgaagg
    30481 caccatgatg atcggtgcgg gcagccttgc cggacttttc attctcgtct tcctgatcgg
    30541 gccgtttgcg ccgaagtttc tgcccatcgt ccgctatgtg cttactccgc acatgcactg
    30601 gattctctgg gtcatcatcc tgttcatgct gatgaccgag tggccgaaga acggtaactg
    30661 gggcccggca ggctggcgga aattttttgc cgcctggtca acgctcggcg cagggctgct
    30721 gactttcttt ctctccggac tgctcggctt cctgcttctc taccgtccgc cggtttccgt
    30781 tgataacgct ttccagaaca tcatgcctgc ttttgtcgga ctctttgccg ttccgtggtg
    30841 cctgctcaat gccgtcagcg ggacggaagt tccgaagcag tacatccgcg acacacttaa
    30901 tatcaacggt gacctgcttc tgcggagcgg catcgcgggc ggtatcggcg gcggctttgc
    30961 cgccttcctg ccggtcgtca ccggcggtat cggcggactg ctcgccgggc acgccacggc
    31021 gcagcgcgac gagcgggtat ttatgatgtc gcaaggcgtt tccaaagtcg tctactacgt
    31081 cggcgcgctg atgctcttct ttgtgccggg actttatctg acgcgtggcg gcggcgcatg
    31141 gattattaaa ggactctata cgccgcacac ccggggcgac tactgtctcg cgctcggctc
    31201 catcgcactt tccggtgcgg tttcctttct gctgctgtcg ccgctcacca ggtggacgct
    31261 taagctgatg gggaaagtgg actaccgcca catctcggtc ttctcgctcg gcatcattgt
    31321 tgcacttgtc ttttcaatca ccggcaggcc gggacttttc attatgactg taagcacggg
    31381 tatcggtctc attcccgttc ttttcggatc gcgcaggctg aactgtctcg gcgtactgct
    31441 gctgccgatc gcctgcaaca tgtccggtat cggcgaaccc gttgccgcct ttttaggact
    31501 gctctgacat gaaaggaatt gcccatttta tttccggagc tgccgtggcc tctttctgcc
    31561 cgtgggcggt tgaggccgcg cagaacggca atcctgcttt tttcatcctt ggcgcggcag
    31621 ccggaattct gccggacacc ttggatttta aattctaccg cttcttctat cgctatgacg
    31681 tttccgttta tcctgatccg gaaaaacttg atccgcaacc ggtcgctgac gctgttgccg
    31741 ccgctgtttc tgaggccgcc gcaaccggcg ggccggtacg cgtgaaactt tcgacgatta
    31801 aactgagcgc cgataactgg cggcagtatc gcgtccggat cgatcccgat gccggtgaag
    31861 tgcgggcaca gttcgggccg gttgtcagca cagggcaggc gccgcagccc ggcaccctgc
    31921 cggcgagtcg tccggccgct gttgcgaaat tttctgcgcc ggtcaggcag agttatgaac
    31981 cggtgtacac cgttgatatt ttcgacggcc cgggattcct ttttaaacgc aacggggccg
    32041 gtgaagtaga aattgatttt ctgccgtggc accgcagctg gtcgcattca tttaccgtcg
    32101 cttcgttgct ggcgggactg gcgtcgctct ggacgtggga ggcggggctc gtcgttttcg
    32161 gtgcttatgc ggcgcacatt attgaagatc aaatggggta catgggcacc aacctgtttt
    32221 tccccttcac caaaaaacgc accgccggtc ttaagttgat gcattccggc gatgcgtttc
    32281 caaacttcac ctgcgtatgg ctttgcggcc tgctaatttt ctggaatatc tatgctgcga
    32341 ttcctgaacc gctttatcac ttcagcttca tgcgcttcat gacatacgcc ctgattatcc
    32401 cgtttgccgt tttcggtgcc gtacgccggt tcctcgttcg cgcgggcaac ggccggcccg
    32461 aatctggtgc cgtgaaatgg gacatctaga gcggaatgca aaaattctgc agcgtttcta
    32521 acgcggctct agctgctgcg gttctgaagg tgatccagaa ctttcgtcag ccaccaccac
    32581 aacccccagc cgatgacgac catggccacc gaaaacagac cataggaaat ccacggattg
    32641 atatttttgg tcattgcgct gaattccgac attccgccca tgctggcgat aatgttgatc
    32701 ggaacaaata ccacgttgat aagggtcaga tttttcagca atacgttcat gttgttgctg
    32761 acgatggtgc cgcgggcatc catgagtccg gacagaacgg tcgaataaat ccccgcctgt
    32821 ttgctggcct gctggttttc gatgatcaca tcatcaagaa attcgatctg ttcggttgag
    32881 agcttcagtt tgccggaagc ggtatggatt tttgacagaa ccgcaagatt tgtttccagc
    32941 gcgttgtggt aataaatcag gctttctccg agcgcgaaca tctgcaggag atatttgttc
    33001 tccatggatt tgatgatctt cgcctgcagt tcctgcgaca tcattttgat ggctttaaga
    33061 tggccctgat agtgatgcac ggtatgcagg agcacccgca ggacgcagtc ccagacggag
    33121 cctatctgct tgaattcccg tccggatatg gggaggtcgt cctgggggac gataaaaacg
    33181 accttgttgt ctttgattaa tataccgagt gatgacacct caaattgaat cgtttcatcg
    33241 cgggtgatgt tgtcgggacg tttccagatc atgaacagcc cgccgtcctg catatattct
    33301 acgcgcggga cttcatcgga gtcatagatt gcctctacgt caaaaggatc taaatcgaac
    33361 ttcgtttgaa gagcttcctt ttcggggtcg gtgggctggc agtaaacgaa aatatccgct
    33421 tcattctcgg cgcattgcat cagccgcgcg gatgctatcc ggtaagtcat tatcatgaaa
    33481 tcaccctgtt tctttccgca tcaagatttc atcgtttcaa atacccagtt cgactgggtt
    33541 gctttgtata tcgaatgttc cggaaacagc aagcgattcg ttcggaaatt cgcggcggcg
    33601 gaaagagcta ctgcttgccg gtctgttcaa gggctttggg cgcggagaaa atgatatggc
    33661 tgagcatggc gcgccggaga gaattcctgt cggaaggatt caggattttc ccaagatcag
    33721 acgatttccg gacagaagtt tccgagccct ggaaactcag attcatcgtc ggcagtttga
    33781 tcgacggaac actgttgcgg aaggcggaca tcttcgggcg gaggtcaggt tccggaagtg
    33841 ccgccggttt cgcggccggt tccggagcag gggcttcgtg gcgcaacggc tctatatcgg
    33901 gcagcgcttc aatgtcgccc ggtttccacg gtgaggagga gcggaaagcc ggtttatgct
    33961 gcggttcttc gatgaagacc ggcggttcaa tatgtaaatt ttctgctccg gccattttcc
    34021 ggagcagctc agcaaacgga tcttcagtag cagctccggt ttcttcctct tcggcgaccg
    34081 ggcgcggcgg ctggttcttc ttggctgctg cgccggcgat ctgggcgatg acccagaata
    34141 cgccgacgat cagccagacg attccaccgt ccgctttgac gaagaggtca gtcatgatta
    34201 ctccttctta tgcgcctgtt cgtcgccgcc ggccagcgct tcgcgcatcg aggtatcggc
    34261 catgacgttc tgcatgcggt agaaatccat aacgccgagg ttgccttctt tgaaggcctg
    34321 ggcgatggcc agcggaacct gagcttgcgc ttcaatcacc ttggcctgca tttcctgaac
    34381 gcgggcgcgc atttcctgtt cctgcgcaat cgccatagcg cggcggcctt ccgcctcggc
    34441 ctgacgcagc ttcttgtcgg cttcggcgcg ttcggtttcg agcatcgcgc cgacgttggc
    34501 cgtattggtc gttccggcga cgctgacgtc ggcgatatca atggagacga tctcaaacgc
    34561 cgtctgcgcg tcgaggccgc tgtcgagaac cttgcggctg atgttgtcgg gattttcgag
    34621 aaccttttta tacgagtcca tcgagccgac agcgctgacg atgccttggc cgacgcgggc
    34681 aataatggtt tcttcggtgg cgccgccgac gagctgttcg agattcgtac ggacggttac
    34741 gcgcgcttta atcagcaggc ggataccgtc tttcgcgacg gcgtcgagga aagtggttcc
    34801 ctgtttcggg ttcgggcagt cgatgacctt cgggttgacg ctggtggtga cggcgtcgag
    34861 aatgtcgcgg ccggcgaggt cgatggcggc ggcctgctgg aagctcagtt cgatattggc
    34921 cttgtcggcg gcgatgagcg cctgcaccac gttgacgacg ttgccgcgcg ccagatagtg
    34981 cgcttcgagc aggtcgctgc tgatatcaat tcc
//
LOCUS       DAOPZO010000006        34511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_1167_length_34511_cov_10.244863, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000006 DAOPZO010000000
VERSION     DAOPZO010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 34511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34511
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1167_length_34511_cov_10.244863"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1628)
                     /locus_tag="PKI68_00845"
     CDS             complement(<1..1628)
                     /locus_tag="PKI68_00845"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNX52271.1"
                     /translation="MAMLITRFHKLIQSKVLWYFILGVIIVSFVGFFMPTNRAGARTH
                     DIPPVGELFGKKVPQDEYRRAYHNSYMWYILSSGRMIPMTDELTKALKREAWQRVAVL
                     HKAKSEEILVADAEVVRQMQAMPLFQAKNGAFDVNIYKAVLQQIGLTPRQAEELFREQ
                     IAIYKLMYQPAQAALISPYELKHAYHLYTDRFILDYVVIPRSQVEKTVTVSKESAQAL
                     FAENPEAFRMPAKVRVSYVEFAVSNFLSKVQIPEDMALQVYNRNIENYRIESTNVNAA
                     AAYKPFEKVKGEITKQLQQISARKLAVEKATEFVVDVAPKTEGGQPDFAGAAAKAGLK
                     IKTLPAFGLKEDLKGIDESAPFRQAAFSLENDAYSSFSDAIIGKDTVYVLSLVQRYPS
                     FVPAYNAVEKEVMDAAREQAVTKALAERAMDVEKAVVKAMSAGTGFKAAVAPFGLKVQ
                     TTAEFDISTELKDKYADTLVKLCLTVPQGRLCDPAAVENGVLLACVTSRKSTDEASGL
                     PAIRQELAGSLSRARAQRLASAWQESLLSEGNFK"
     gene            complement(1713..2963)
                     /locus_tag="PKI68_00850"
     CDS             complement(1713..2963)
                     /locus_tag="PKI68_00850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HNX52272.1"
                     /translation="MKNLNEKIINQQTRLAAEILETLTPHVLEHRHPADRVLLRFFKN
                     RHELGSRDRRFLSECFFSYFRWLGWTKALQLPPIEAAALSWLLDQTGLHPALAAAAKS
                     GWTPLGGKTLEEKRAALVSWFPDFQGLDKADLIFPLFGKSVDLPAGFEDSFYETLQQR
                     PPTWLRLRSDEFRQTLAEAGIAFKEHPVLSGAISVEAGTSLGKLGHSGQFEVQDIASQ
                     SVARIAGPALGSDWWDGCAGAGGKAVQLADLIGHDGKVLATDLRTEALHECKKRARAG
                     GIMNIRTQLHDLAKDKPFTKEFDGVLVDAPCSGWGTWSRNPDARWRSDPRDPAQKRNL
                     QVKMLNNAVQCVKSGGLLVYSVCTFTREETVEVIERFLPEHPGFALEPFDHPLTGKPA
                     NGAVQIWPWEGPGDGMFIARLRKR"
     gene            3073..4233
                     /locus_tag="PKI68_00855"
     CDS             3073..4233
                     /locus_tag="PKI68_00855"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNX52273.1"
                     /translation="MAKELGFVMITPHSLRKSRTGGIIGRFSRIEGLEFVASRMFGPS
                     QELVNRYAELLRTNPDIKPESRDILADYVLRAFGPETDTGRPHRVMLLLFEGEDAIKK
                     IYAAAGGFDDTQESSDTVRGTYGDCIRDSAGKVIYFEPSVLVAPTASSAKQTISLWIN
                     YAESDGGIVKNALSLPDADVERTLVIIKPDNFRFPSSRPGNIIDLFSRSGLRIVGAQV
                     HRMSMAEAKEFYGPVQAVLREKLCGRTTEKACVALEEEFGFAMPAEVKDALGKTLGPY
                     YGDQQFFEIMKFMTGLWLPDVPASEMNADGKVRCLVLIYAGVDAVNKIRNILGPTDPS
                     KAQPGSVRKEYGSDIMVNAAHASDSAENAKREMGIIKPERNLLKQWFDQYYK"
     gene            4325..5503
                     /locus_tag="PKI68_00860"
     CDS             4325..5503
                     /locus_tag="PKI68_00860"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF006719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HNX52274.1"
                     /translation="MWKNVTPAPEDAILGLTEAFKKDPRFAKINLGVGVYKDEEGHTP
                     ILDSVRAAERQIFEKENTKSYMPIAGEKIYGEEVQKMIFGSLCDCSATIHTPGGTGAL
                     RVAADLLARFSSKTIWVSNPTWANHNNIFEAAGLTVKSYPYYNAVTKDLDADGFFATL
                     EKIPAGDCVLLHACCHNPSGVDLSAAQWKQVAAIAQKNGWSALVDFAYQGFGESVEAD
                     RVGVEALLDSGIEFLIASSFSKNFGLYRDRTGALTAVTATPADAATAMSHMMAMARVL
                     YSNPPAHGGMIVTTILRDKELCKLWRSELDAMRDRIAQARAALAQGLTERGVPMDCSF
                     MTKQKGMFSFSGLNKDQVKFLREEKAVYIVGSGRINVAGLTPGNMGFVCDVVAEAMKR
                     "
     gene            complement(5507..5965)
                     /locus_tag="PKI68_00865"
     CDS             complement(5507..5965)
                     /locus_tag="PKI68_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52275.1"
                     /translation="MKITERCLTDYFSRSTLCATEDKLVWHYKSLKLEFTREYSYADL
                     SPITKKMKVGVTNAANCSILLFVISAPMSILANRTSHYLYIAVLLFLIASAFVFLLQF
                     KKHEEIFFYTKEKEFAFSFSEEHHQEFLAVVMDKIIDKTSATASVSEAKK"
     gene            complement(6089..6982)
                     /locus_tag="PKI68_00870"
     CDS             complement(6089..6982)
                     /locus_tag="PKI68_00870"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNX52276.1"
                     /translation="MKSPMLPSEHQDAVQRALRLGRSGNPAALPELIELSHLPSNEVQ
                     RLAASAIGKLADFGADAGKAVAVLAPLALHGRHPQTQQYAIRALKKYGAATQSQVPDL
                     RDLARNPANPNYVRAAAKTAADAIELAAQDAGAGVKHRCKRCNAAVFPEEYARANEVF
                     QRVFCDRCFDEVFLERRNFETQVEINKTIEARDGTLVQSEGERRVAEWLTEHGLSYRY
                     DAKYRVIGEFQIRPDFYLPEVDVYIEYWGLDTPQYKMSMYKKQTLYQQEGKRLVSVYP
                     KDLPVLDNLLTAKLRLFGLSL"
     gene            complement(7095..8501)
                     /locus_tag="PKI68_00875"
     CDS             complement(7095..8501)
                     /locus_tag="PKI68_00875"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX52277.1"
                     /translation="MSIKLNFFGAAKNVTGSCYLVESGGSRILVDCGLYQERDLKERN
                     WDAFPVPPATINAVLLTHAHLDHCGRLPKLVKDGFRGKIFCTSATADIAQIIMKDSAV
                     LQEEDIKHKKARHEKQGKTSPFPYEPLYTAADVEKTVPLLTPVGYKTPVAVAAGMTAS
                     FREAGHIFGSASIKLTAGQGGEARSILFSGDVGRWNMPIINDPDQFEDADYVLVESTY
                     GDRLHGETKDIPGELARIINETHKAGGNIVIPSFAVERTQELLYHLNGLLRENRIPHL
                     FTFVDSPMAIRVTEIFKKHPELFDEETRDLLRKGIHPCDLPGLTLSNTVDQSKAINHI
                     KGTAIIIAGAGMCTGGRIKHHLKNNISRPESTILFVGYQAAGTLGRAIQDQPAEVRIF
                     GEMQPLRARIEKISGFSAHADRDELLRWITGLKKAPRKVFITHGEPDAAVAFKNFLTG
                     KTGWSCTVPEYRQEIVLD"
     gene            8609..8690
                     /locus_tag="PKI68_00880"
     tRNA            8609..8690
                     /locus_tag="PKI68_00880"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8643..8645,aa:Leu,seq:tag)
     gene            8744..9040
                     /locus_tag="PKI68_00885"
     CDS             8744..9040
                     /locus_tag="PKI68_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52278.1"
                     /translation="MLPQHGGVPNFSQRIPAPILTLFILALITGLLLTLSFYLTADEG
                     DLAAQSRVKLSLLVTLLVSFFILLSGTGRWWRPHLWNHKSGHRPHRRRRSHHRH"
     gene            complement(9138..9926)
                     /gene="nagB"
                     /locus_tag="PKI68_00890"
     CDS             complement(9138..9926)
                     /gene="nagB"
                     /locus_tag="PKI68_00890"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF001684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HNX52279.1"
                     /translation="MEIIIQKDALSGSRAAARVVARKIREKPETVLGLATGSTPLLLY
                     KELIRMHREEGLDFSSVTTFNLDEYVGLPADHPQSYHYFMHENLFNHINIKPKNIHIP
                     DGMTKDIPAYCAAYEKAIVKAGGIDIQILGIGSDGHVGFNEPTSSFASRTRIKTLTRQ
                     TVADNARFFGGNESNVPHHCITMGIGTIMEARMNLLLAFGESKAAAVAAMTEGPVAAI
                     MPASILQHHACAKIFIDEAAAAGLKHKDYYSWVYEGKPDWQRDI"
     gene            complement(9961..10770)
                     /locus_tag="PKI68_00895"
     CDS             complement(9961..10770)
                     /locus_tag="PKI68_00895"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNX52280.1"
                     /translation="MNNPYLEYTRQMESLVKKAGTMPCGGMPPCLRPALPPDAPCVLL
                     FSPHPDDECITGTLPLRLMREAGMRIINVPVTFGSNSARQPGRAEELENACRFLGWDI
                     QRRSGFLNLEKSDVVEILKTVQPAVIFVPHSKDWNSRHIATHFIVMEALAAMPPDFSC
                     TVVETEYWQAMEHPNLMVEADAQTVADLIAATSFHVKEVERNPYHLTMPAWMQDNVRR
                     GGELVGGQGGAAPDFPFATLYRLRRWCAGGLNDVFNNGRMISMETDLKTLF"
     gene            complement(10783..12165)
                     /locus_tag="PKI68_00900"
     CDS             complement(10783..12165)
                     /locus_tag="PKI68_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52281.1"
                     /translation="MNSTQLKKSRLAVPTPLDPEFLPAILFERDYLQKLEKTEELIIA
                     VERENGCVSRKKLLVATESSGLRTETLLYVDRVVKFMLWASGGWKLYIGGAEWIGREM
                     AKTYSSSGPRAFDVALMERVYDRPFETVCCAPGKIPADKENEEAIGGHLAGCRIGFDL
                     GASDYKLAAVKDGQPVFSTEIPWDPVKQADPDYHYQAIQKGLKLAAEHLPRVDAIGGS
                     SAGVFVDDRPMVASLFRSVPAGLYETKIKNMFHRLREEWGVPLTVINDGDVTALAGAM
                     SLKKEGMFGIAMGSSEAVGFLDRAGHVTGRLNELAFAPVDFNPAAAADEWSGDRGVGA
                     LYFSQQAVNKLAPAAGFSFPAETGLPERLKAVQVEADAGNEAALKIFETIGVYLGYAL
                     PWYARFYDFEDVLILGRVTSGRGGEKVIETARAVLAKEFPDFSARLHVPDEQSRRVGQ
                     AVAAASLPQI"
     gene            complement(12276..13457)
                     /locus_tag="PKI68_00905"
     CDS             complement(12276..13457)
                     /locus_tag="PKI68_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HNX52282.1"
                     /translation="MARRDLPSYIRSRLSFEDLSSSRSILQLIDRQGAVTQVQAAEAV
                     GLSSGTGNLHFQKLEHMGLIRRADSIRGGGRGRATIVWELEQDKNLCLLLVFDVPFFQ
                     ATLVDFKGNVVLQKRQDLTGIADSRALTAEVENFVNAAVVKAQRIGGVIRQAFAGVPG
                     LFEPKSSVIRNAANFPALNGIDFRRWMQEHYDLPCFCGSLGLAFYYGEARRLAADTRT
                     MVVFWDLGVGAVAGVGERIISHTGDSLLSEIGHIRVRRNGRPCRCGRRGCLEAYSGGW
                     AMIEALQDKKIKSLEELRSAVLSGCPDAVKAACDAARLLGSSLCWPLQIMQSERLIIS
                     GPLSSVFPAVRSALLEGLASTFEDEEIAALDPAASEDAALAMQTGAYLFARRLFFYPE
                     V"
     gene            13733..13808
                     /locus_tag="PKI68_00910"
     tRNA            13733..13808
                     /locus_tag="PKI68_00910"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13766..13768,aa:Lys,seq:ctt)
     gene            complement(13904..14896)
                     /locus_tag="PKI68_00915"
     CDS             complement(13904..14896)
                     /locus_tag="PKI68_00915"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNX52283.1"
                     /translation="MCRKNEKEVLRLLKKAGLTVHNLSDVPLRTIRHAQRGAFPDMRM
                     AHLAPGLRDEALVAPILQDLLCTRVGFQGPSAAHYIPRPLGSYDCILIFCTEGRGWLE
                     INGQSWDVTRNEAFLIPQHIPHTYGADPDDPWSNYWVHFQGRQAADFARLITRPGESP
                     VIHLSARQEVIAAIEQLYEYMGQVHTSSTLVAASGALNQLLGLIQLRMLASEGKCRTA
                     EESLDKTVEFMHRNLSKKLSLKELAKISGMSPNHFGALFNRRYEHTPMDYFSRLKIQK
                     ACELLTTTSLRISEISETLGFADSYYFSRLFRKIMGLSPRAYRAGETVSDASSI"
     gene            15118..17457
                     /locus_tag="PKI68_00920"
     CDS             15118..17457
                     /locus_tag="PKI68_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HNX52284.1"
                     /translation="MDVTKTCCQTAAGGAKTERVYAADLHQSRFKPNDRLKKLVDRRD
                     WLAKNHKRDIYCDNAEFLIYHWSGKAETFAAATGEQAASEEERINIGDHTCRVDVGGP
                     FPYAAQMPSKVNGYEPTVTNWAADYAFFLRHSEAEVFPYEQIVGEFHWQLDEARFYKY
                     PKSHADLGLKARELGAGGFSLAHCCPDLSIGLSLGWGGLLEKVRASREKHRKFGNEKA
                     VTYLDASEQVVLSVIDYVQRHSEKAARLAAVETDPVKKTSYEMVAENCAAIARGAPAT
                     YHQALQWIFLYMMVERINGHGNGYGRFDQLLMPFYEKDKAAGRIDRNRARELMAEWYL
                     KYGPHLSFGGRLEDRSDATNEMSWIGLEAYDMVGGYNQFGVQWHADMDPEFFKYCCDV
                     VGRHGCGVPALLNSDVMVASQLRTGFEEKHALNTSYSGCQWYCIPGREYQDQDVNCIV
                     LISPMQRAIDMAIEQKVSDWNGFWAIYCREVDRSGEALVAFKNEAYKYHAQLWPEMIT
                     SLVSYGPIEKGRDITDFDAVDYNYPSVNVLGVPNVVDSMYALKRAVFEEKRFTLQQVR
                     DACARDWSGDGEEAMRQYLRNLPKFGNDIDDVDAMAVQISEQVRETVESKRCLKGGHF
                     RASLFQYQGHTVAGPYLGATPDGRKRREPLAHGMNPMHGRNTNGLSATANSFCKLDFS
                     KYQGGSFQIELHPSFFAEGFDRGQLVDIFARAFFKKGGVQINLNVFDLDDLKEAYEHP
                     EDEKYEDIVVKVTGYSAHFTKMDRLFQKEFIERVNYESL"
     gene            17492..18418
                     /locus_tag="PKI68_00925"
     CDS             17492..18418
                     /locus_tag="PKI68_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HNX52285.1"
                     /translation="MLRLQTHDGPGLRTTVFMKGCSLRCAWCHNPESLSGAQQVWHFP
                     SKCIGCGACQQACPSGAIAVRPGGLEILRDRCTGCARCVAVCPARAMEALRTEWTPDS
                     LMDLIRRDLAFLSEGGGVTFSGGEPALQAPFVAEVLKRCRAAGLHTALDTCGAVDRSA
                     YDQLLPHCNLVLFDLKLADSEAHQRWTGQPNGRILENLRHVAETAGQRPDLELWVRTP
                     LIPGATATPENVGAAARLLRDLPAGSVSRWELCSFNNLCADKYSRLGSPWCFTGEPLL
                     QRSCVSALRQQAAAESGLQETQVHLRGRWQDG"
     gene            complement(18527..19960)
                     /locus_tag="PKI68_00930"
     CDS             complement(18527..19960)
                     /locus_tag="PKI68_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNX52286.1"
                     /translation="MKKCAHLISSSLAFTAGLAGGASQADLRPNILWIMVDQQPATAM
                     SCAGNPDLKTPAMDSLSAAGIRFERAYSVNPICVPARTAMMTGRMSHQTGVMTNIERY
                     DVAAPSIGKLMTAAGYDTGYIGKWHIPMPTETRSNSWHGFNLMMAGSKAFNDQHFAQP
                     AIDFIKKKRDQPFFLVTSFVNPHDICEWARRATGGFPASRTELWNGEIAATPRPELCP
                     ALPANFGIPANEPDIIREYQATQKSAYPVIGWPDERWRQYRWALNRLTERVDSEIGKI
                     LQALRAEGLDKNTLILLTSDHGDGNGAHQWNQKSLFYEETARVPLILSGPGVKKPGTV
                     DTEHLISTGLDLFPTFCDYAGANRPGDLNGLSLRPLAEGRAAPWRTRLVSETVLSPRD
                     NLPGPEGRMLRTKDYKYCVFSAGRIREQLIDMNKDPGEMHNLALDPDYASVLQQHRQQ
                     LADEMKQTGDLFVVPGISYAGWILKAE"
     gene            complement(20229..21479)
                     /locus_tag="PKI68_00935"
     CDS             complement(20229..21479)
                     /locus_tag="PKI68_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HNX52287.1"
                     /translation="MNKLNWCVLLCGLSMSVAGVGESTSCRYEAQPVDLKVIQDNGKI
                     RVEGTSVFQLPGDFVWCGSPIRIKDQYFMFYSTWESGPSIPPFADSWVLFSKVGLAVS
                     DSPYGGYRSLGLVLKGRKDQGDPTAWDAQMVHNPLIKYFNGKYYLYYCAGKDPGIQPK
                     GSPGENVTKRNRVQQSQQIGVIEFNSVEDLLAGRFTRFDQPLVSPRTRVKSKAVVDPS
                     PPGTIAGPDNLIAVNPAVEYRPADGKYLLFFKGNIYDPNWRGVHGVAVGDSPTGPFTT
                     TDRFVFDVDDGKGHKVSAEDPYVWYHHKDKLFYAVIKDFTGKLTRGGPGLALMQSVDG
                     MDWTLAPSPLFMKKELILKDGTRLKVARLERPQLLLDENDDPLALYAACSINDANPKR
                     DGGTFNVQIPLKKIKPAQEPESKP"
     gene            complement(21617..23263)
                     /locus_tag="PKI68_00940"
     CDS             complement(21617..23263)
                     /locus_tag="PKI68_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNX52288.1"
                     /translation="MKRKNESIRLPLTSRLMLGLAVSAGLTQAGDALAAGKPNVVFVL
                     ADDLGYGDLSCLNPAGKIKTPNLDRLSREGMIFTDAHSSSAVCSPSRYSVLTGRYNWR
                     SRLQKGIVPVYGNPLIAKDRLTVPGLLKQYGYHSACIGKWHLGMGWDFDAADGVFLSK
                     NGKNPPVTPELQAKWQAAFSKPTTGGPTTRGFDYYFGVDVPNWPPYCFIENDRTVGIP
                     STWLSERLLGNNQAGPIGPAMPYWNFEQLLPTWAQKADDYIKARAAAKEPFFLYMALT
                     SPHTPISVNKSFIGKSGLNNLYADFVIETDDVFGRILDSLKRYGVEDNTLIIFSSDNG
                     CAHYIGTYVDAAGFHKAMEPQGHFSSGPYRGYKSDIWDGGHRVPFIARWPGVIAPGSR
                     SDELVCLSDLMATCADILGAALPATAGEDSVSLLPLFRNPASPVRDHVIHHSINGQFA
                     IRDGRWKLVLCPGSGGWTLRDADAAKDGLPMQQLYDMKNDPGEKTNRYAQEPEQVKKM
                     LAQLQKDIDSGRSTPGAPQTNDTRVIIWKADASEKPQKDH"
     gene            complement(23376..24272)
                     /locus_tag="PKI68_00945"
     CDS             complement(23376..24272)
                     /locus_tag="PKI68_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX52289.1"
                     /translation="MKMDHQKHLSGFRGQILHVISRSILSLLESHKLVRDLRVTDIGW
                     FPDAKNHSIRRPHGAGQHILILCVDGAGWVEGALRRETVSAGQAVLIPAGIPHAYGAS
                     PHAPWSVHWVHFDGEDAPLYLQRMPASSFCISVSAECVSAMVSVFCRCYDALAGGYAD
                     RNLLYLSHALRDLLGLLFYNNPAYSPDGKTGSGHNLENIMALMTQRIGQALTVPEMAQ
                     HAGMSVVHFSALFRRQTGLSPGDYFIHLRLKHACWLLETTALPIREIGFKTGYEDPYY
                     FSRSFRKLMGLSPRQYRKMSKG"
     gene            24429..25850
                     /locus_tag="PKI68_00950"
     CDS             24429..25850
                     /locus_tag="PKI68_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HNX52290.1"
                     /translation="MITGSAAVSSSGTKADYNLGYVWLISLVAAMGGLLFGYDWVVIS
                     GADIFYEKFFNLVSASEIGWAKSCALVGCLLGAVASGVLSDRFGRKRLLVLSAVLFTV
                     SSLGTGLANTYDTFVAWRIIGGVAIGLASNLSPMYIAEIAPAAIRGKLVSINQLTIVI
                     GILLAQVANWLIAQPVPPDASAQEILNSWNGQQGWRWMFAATAIPSLLFFLGMFFVPE
                     SPRWLAKKGQRSRAVSVLSRINGTAGAEQAITDIDATLANETGRIDFRELLQPKLRKV
                     LLLGIALAVLQQWCGINVIFYYTKDVFAAAGYKVSAMLLNIIIIGLANLVFTVVAIQS
                     VERLGRRFLMLAGWAGLTVIYLLLGGSYFFHLPGLIPVILVIAAIGCYACTLAPMTWV
                     VLSEIFPNRIRGTAMSIAVFALWTGCFTLTYTFPALNAQLGSSGTFWLYAGICAAGYF
                     FCRARLTETKGKTLEQIERELVD"
     gene            25973..27229
                     /locus_tag="PKI68_00955"
     CDS             25973..27229
                     /locus_tag="PKI68_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNX52291.1"
                     /translation="MTSRERIEATCAHKQPDKLAVDFGGGFQTGIHVSMVYQLRQALG
                     LDKPGTPVKVVEIYQMLGEIAPDLQDALGIDTVSLCGTGTMFGFPAKEMKEWRMNDGT
                     PVLVPVDFNTVLEPNGDLLQWPENDRSVKPSGRMPAGGYFFDAIIRQQPIDEDNLNPA
                     DNTEEFSPIADSELDHYARRAISLYEQTDRALFCTFGGLTFGDVALVPATFLKNPKGI
                     RDVEEWYITTASQPDYVKAVFERQAEVAIENLARLHQAVGNRISVIQTNGTDFGTQNG
                     PFCSVETYRELFLPYQKKVNGWIHANTNWKTFMHSCGGVWPLLDEIVEAEFDILNPVQ
                     CSATGMDPRKLKKNYGDKLVFWGGGIDTQHTLPFGTPDEVRAQVRERVEIFNEGGGFV
                     FCSIHNVQACTPVANLLAMFDAMRNP"
     gene            27256..30060
                     /locus_tag="PKI68_00960"
     CDS             27256..30060
                     /locus_tag="PKI68_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52292.1"
                     /translation="MRNTRFAAWLSSIVLGAALAQAADSVSLAGEWRFALDRKDAGVA
                     EKWFSKNLADRIQLPGALQNQGFGDDITVATKWTGGTRVERWLTLPQYEKYRQPGNIK
                     IPFFLQPDKHYTGAAWYQRDIEIPAGWQGRRVVLTLERAHWTTQAWLDDRPLGSNVSL
                     SVPHVYDLGTGLAPGRHRLSIRVDNRLLVDVGARAHSVSDETQGNWNGLIGNLTLSST
                     GPVWIDDVQAYPDIAKKTARVNVRFGNASGRAGSGVLKIGSISRPVSWLENGGSAEVE
                     VALGGDAGLWDEFSPVLQKLNVQLSGDGAMDERQLVFGLREIGTRDRQFVINGRPTFF
                     RGTLECCIFPLTGYPPTDVEAWKRIIRICQDHGLNHIRFHSWCPPEAAFVAADELGFY
                     YQVEVAAWATVGDGAPIDQWLYDEAARILKAYGNHPSFLLMPYGNEPQGKNRERWLGD
                     WVNHWKQTDPRRLYTSGAGWPCIPENQYHINDNIRGSKGWLGRDYQKMLDAVSLKNVT
                     VKTIDVPMIAHEVGQYCAYPDFDEMKKYTGPLKPKNFEIFRESLAEHGMLHQAKEFVM
                     ASGKLQALCYKEDIEASLRTPGIGGVQLLDLHDFPGQGTSLIGILDPFWDSKGYITPA
                     EFRRFYNTTVPLTRLTRHIWTTEETLTTDVEIAHFGAAPFAAAQPYWKVLADDGQVVA
                     AGDLPVQAVPLGTRTKLGRISLALRNLPAPNQYKLVVGLKDTPFENDWNIWVYPAGMP
                     AAPADVLIAESFDESVCRRLADGGKVLLLAAKPGSGNPKLTFLPVFWNHYIWQKREQT
                     LGLLCDPKHPALAQFPTRYFQDWQWYDILSSANGIVLDRLPGDLRPIVQPIDDWNTNR
                     KLGMIFECKVGPGKLLVCAADLSTDLSARPAARQLRASLLAYAASGAFNPKTEVEPSV
                     LTEAFSLAGPPALVKPRKGN"
     gene            30310..30630
                     /locus_tag="PKI68_00965"
     CDS             30310..30630
                     /locus_tag="PKI68_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52293.1"
                     /translation="MLHPVIAFIGSGGGELLVIMLALLLLFGPKDAPRILRSIQNVLN
                     KLQRAAADFRYKLMYTDLHWNQPEAPKDSTACDAEEKPAEAKPAEEPPELPTEHPPAD
                     EPKT"
     gene            30627..31424
                     /gene="tatC"
                     /locus_tag="PKI68_00970"
     CDS             30627..31424
                     /gene="tatC"
                     /locus_tag="PKI68_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNX52294.1"
                     /translation="MKNLFKKLRSRFIYHDKEQSFLEHLEEFRTMLIRCLLTMAITTV
                     ICIPLAKPLLHWLQAPLLQVAAQNHYTFALITTSPIEGFMQVMKVIFATGVLLSLPFI
                     IYFIAQFVFPGLKQNEQKMLIYGGLAGAVLFAAGVALCYTVTLPVAVNVMFYFNDYLG
                     TTAYWKIDTYLGFVMQLLIGFGLAFELPLILLMLGRMGIISTAQMRKYRRHVVIVILI
                     IAMVLTPPDVLSQLQMAIPLYILYELCILILRIRERNMRKEKEGTEE"
     gene            31421..31678
                     /gene="tatA"
                     /locus_tag="PKI68_00975"
     CDS             31421..31678
                     /gene="tatA"
                     /locus_tag="PKI68_00975"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNX52295.1"
                     /translation="MNILALIPGMEGPSEVLLIFLAILLLFGGKKLPELARSLGKSIG
                     EFKRGQKEGHQDAKESEKPELPVPQDQPAAKSGDEKKGAEQ"
     gene            31675..31908
                     /gene="tatA"
                     /locus_tag="PKI68_00980"
     CDS             31675..31908
                     /gene="tatA"
                     /locus_tag="PKI68_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNX52296.1"
                     /translation="MNCLAIFGIESPAEILLIIFIFVLLFGGKKLPELARALGKSINE
                     FKRGQTESEPEKKIEKQAEPADPDEPDSTASKK"
     gene            complement(31909..33465)
                     /locus_tag="PKI68_00985"
     CDS             complement(31909..33465)
                     /locus_tag="PKI68_00985"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNX52297.1"
                     /translation="MPIKSDYAEQSEKELAILYQISQAAATHPHTVSELLIEVLDIME
                     TELEVSRGTFTLRKPDTEIFVIEASRGLTAEEKKRGQYKLGEGVTGRVAQTGQSALIP
                     DISKDPRFLNLTKSRTGNPTAFICVPVIYRKAVIGTMSIDLSITTDLEELRRYQRFLE
                     LVANILAEAVASIREELEEREGLLTENERLRRQLGDRYRMHNIIGNCSAMRSVYEQIV
                     QVANSTATVLIRGESGTGKELVARAIHYGSERKNNAFVSVNCAALPENLIESELFGHE
                     KGAFTGATQQRKGRFETANGGTIFLDEIGDISPSVQVRLLRVLQERTFERVGSSESVS
                     VNVRVLAATSRNLEKEIAEETFREDLYYRLNVFPIILPPLRERRSDIMLLADHFLQKY
                     GEMYGKNIKRISTSAINMMMAYHWPGNVRELENCLERAVLTASDEVIHGYNLPPSLQT
                     SDETQTSIFPKDGADLKTMVESYEKEIIIDALKKHRGNAAATARYLNTTQRIINYRIQ
                     KLGVNPDDYK"
     gene            33752..>34511
                     /locus_tag="PKI68_00990"
     CDS             33752..>34511
                     /locus_tag="PKI68_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52298.1"
                     /translation="MNKKEEFQKGFPEPQGLYHPENEHDACGVGFICNLHGKKSHDII
                     HHALEILVRLTHRGACGCDPLTGDGAGILLQIPHAFYKIECAKLGIELPAEQEYGTGL
                     VFLPQDEVQRKTCMEILEQAVKEEGQKLLGWRKVPTCNRLIGETARASEPYIMQIFIG
                     RGKGVSDPRTFDRMLYIIRKVAENRIFHSGLKDKNDFYIPTLTCRIIIYKGLLLPEQM
                     EFYYPDLLDDNFTSALALVHQRYSTNTFPAWKLAQ"
BASE COUNT         7351 a         9957 c         9760 g         7443 t
ORIGIN      
        1 tctttgaagt tgccttcgct caaaagcgat tcctgccagg cggaagccag gcgctgggcg
       61 cgggcgcggg acaaactgcc ggccagttcc tgccggatgg ccggaagacc ggaggcttca
      121 tcggtggact tgcgcgaagt cacgcaggcg aggagtacac cgttttcgac cgcagccgga
      181 tcgcagagtc tgccctgcgg aacagtgagg caaagtttga cgagcgtgtc ggcatacttg
      241 tctttcagct cagttgaaat atcaaattcc gccgtggtct gcactttgag cccgaacggg
      301 gcgacggcgg ctttgaaacc ggttccggcg gacatggctt ttacaacggc tttttccaca
      361 tccatcgcgc gttcggccag cgctttggta acggcctgtt cacgtgccgc atccattact
      421 tctttttcaa cggcattata tgcaggaacg aaagaaggat aacgctgtac cagcgacaga
      481 acgtaaacgg tatctttacc gataatcgcg tcgctgaagg agctgtaggc atcgttttcc
      541 agactgaaag ccgcctggcg gaaaggcgcg gattcatcaa tacccttgag gtcttctttc
      601 agaccgaacg ccggcagcgt tttaatcttc agtccggctt ttgccgcggc tccggcgaag
      661 tcaggctggc cgccttccgt tttgggtgca acgtccacca caaactccgt ggctttttca
      721 accgccagct tgcgggcgga aatctgctga agctgtttgg tgatttcacc ttttaccttt
      781 tcaaacggtt tgtaagctgc ggcggcgttt acgttggtgc tttcgatacg gtagttttcg
      841 atgttgcggt tgtagacctg caaagccata tcttccggaa tttgaacttt cgacaggaaa
      901 ttgctgaccg cgaattcaac ataggaaacg cgcaccttgg ccggcatgcg gaacgcttcg
      961 ggattctcgg cgaaaagcgc ctgcgcggat tctttggaaa ccgtaaccgt tttttcgacc
     1021 tgcgaacgcg ggattacaac gtagtcgaga atgaagcggt cggtataaag atggtaggcg
     1081 tgtttcaatt cgtaaggcga aatcagcgcg gcctgcgccg gctggtacat cagcttataa
     1141 atggcgatct gttcacggaa aagctcttcc gcctgacggg gtgtaaggcc gatctgctgc
     1201 agcaccgctt tgtagatatt gacgtcaaac gctccgtttt tcgcctgaaa aagcggcatg
     1261 gcctgcatct gccggacaac ttccgcatcc gccacaagga tctcctcgga tttggcttta
     1321 tgcaaaactg ccacgcgctg ccaggcttcc cgtttgagcg ccttggtcag ttcatccgtc
     1381 atcggaatca tgcggcctga agacaggatg taccacatat aggaattgtg ataagcgcgg
     1441 cggtattcat cctgcggcac ctttttaccg aacagttcac cgacaggcgg aatgtcgtgg
     1501 gtccgtgctc ccgcgcggtt cgtcggcatg aaaaatccga caaaggagac aatgatgact
     1561 ccgaggataa agtaccacag caccttggac tgaatcagct tgtgaaagcg cgtaatcaac
     1621 attgccataa acgacccctt ggttttgcac taaaaaaaca ggggcacagc atagccatgc
     1681 cccttcgccc ggtcaacggg aaagccagct cctcagcgtt tccgcaaccg ggcgataaac
     1741 ataccgtcgc cgggaccttc ccacggccag atctgtaccg ctccgtttgc cggttttccg
     1801 gtcaaaggat gatcaaacgg ttccagcgca aaccccggat gttcgggcag aaaccgctca
     1861 atcacttcaa ccgtttcctc gcgggtaaac gtacaaacgg agtaaaccag cagaccgccg
     1921 gacttcacgc actgcacggc gttgttcagc atcttcacct gcaaattacg cttttgcgcc
     1981 ggatcgcgcg gatcgctgcg ccagcgggca tcgggattac ggctccacgt accccagccc
     2041 gaacagggcg catccaccag aacgccgtcg aactccttgg taaacggttt gtctttggcc
     2101 agatcgtgaa gctgggtacg gatgttcata atgccgccgg cgcgcgcgcg ctttttacac
     2161 tcgtgcagcg cttcggtccg cagatcggtc gccagcacct tgccgtcgtg accgatcagg
     2221 tcggccagct gaaccgcttt gccgcccgca ccggcgcagc cgtcccacca gtcagagcca
     2281 agcgccggtc cggcgatccg ggcgacggac tgcgacgcaa tatcctgcac ttcaaactgt
     2341 ccgctgtgac caagcttgcc gagcgatgta ccggcttcga ccgaaatcgc gccggaaaga
     2401 accggatgtt ccttaaaggc aattccggct tccgcaagcg tctgcctgaa ttcatcgctg
     2461 cgcagccgca gccacgtcgg cgggcgctgc tgaagggttt cataaaaact gtcttcgaag
     2521 cctgccggca gatccaccga cttgccaaac agaggaaaaa taagatccgc tttatccaag
     2581 ccctgaaaat ccgggaacca gctgaccagc gcagcgcgct tttcttccag cgttttaccg
     2641 ccgagcggcg tccagccgga cttcgccgca gcggccagcg ccggatgcag accggtctga
     2701 tcgagaagcc agctgagggc ggcggcttcg atcggcggaa gctgcaaggc ctttgtccag
     2761 ccgagccagc ggaaatagga aaaaaagcat tcggaaagaa accggcggtc gcgggagccc
     2821 agttcgtggc ggtttttgaa aaaacgcaac agaacgcggt cggccggatg ccggtgttcg
     2881 agtacatgcg gagtgagcgt ttccaggatt tcggcggcca gccgggtttg ctggttgatg
     2941 attttttcgt tcagattttt cacggggttc ctcgcagatt tttgtttttt tgttttaaaa
     3001 gttggacata atccgtgccc caaccagacg gatcaggaga atgcagggaa aagaggacga
     3061 aaggcaactg ttatggcaaa agaactcgga tttgtgatga tcacccccca ctcgctgcgg
     3121 aaatcgcgta ccggcggcat tatcggacgc ttcagccgca tcgaaggcct cgagtttgtt
     3181 gcctcgcgca tgttcggccc gagccaggag ctggttaacc gttatgccga actgctgcgc
     3241 accaatcccg atatcaagcc cgaaagccgc gatattctcg ccgactacgt cctgcgcgcc
     3301 ttcggtcctg aaaccgatac cggccgcccg caccgcgtca tgctgcttct ttttgaaggc
     3361 gaagatgcga tcaaaaaaat ctacgcggcc gccggcggct ttgacgatac acaggaatcg
     3421 tccgacaccg tgcgcggcac ctacggcgac tgcatccgcg attcagccgg taaagtgatt
     3481 tattttgaac cttccgtgct ggtagcgccc acagcctcct cggctaaaca aaccatttcg
     3541 ctctggataa actatgccga aagcgacggc ggcatcgtta aaaacgctct cagcctgccg
     3601 gatgccgacg tagaacgaac tctcgtcatc atcaagccgg acaacttccg cttccccagc
     3661 tcacgccccg gcaacatcat cgacctcttc tcgcgctccg gcctgcggat cgtcggcgca
     3721 caggtgcacc gcatgagcat ggcggaagcc aaagagttct acggcccggt tcaggccgtc
     3781 ctgcgtgaaa aactttgcgg ccgcaccacc gaaaaagcct gcgttgcgct cgaagaggaa
     3841 ttcggcttcg cgatgcccgc cgaagtcaaa gacgcactcg gcaaaactct cggcccgtac
     3901 tacggcgacc agcagttctt tgaaatcatg aaattcatga ccggcctctg gctgccggac
     3961 gtaccggctt ctgaaatgaa tgccgacggc aaagtgcgct gcctggtatt gatctacgcc
     4021 ggcgtcgatg cggtgaataa aatccgcaac attctcggcc cgaccgatcc gagcaaagca
     4081 cagcctggct ccgtgcgcaa agaatacggc agcgacatca tggtcaacgc cgcgcacgcc
     4141 tccgactcgg cggaaaacgc caagcgtgaa atgggcatca tcaaacctga gcgcaacctg
     4201 ctcaaacaat ggttcgacca atattataaa taaaaagtac tgcgtaatcc gtattgcgta
     4261 ttagaaaaga tgtgacacgt aatactcagt acgaaatacg cagtaaaaat ctggagaaac
     4321 agacatgtgg aaaaatgtga cccccgcccc tgaagatgcc attctcggac tcaccgaggc
     4381 tttcaaaaaa gatccgcgtt tcgccaagat caacctcggc gtcggcgttt ataaagacga
     4441 agaaggccac acgccgattc tcgactccgt ccgggcggcg gaaagacaga tcttcgaaaa
     4501 agaaaatacc aaatcctaca tgccgatcgc cggcgaaaaa atctacggcg aagaagtgca
     4561 gaagatgatc ttcggttcgc tctgcgactg ctccgccacc atccacaccc ccggcggaac
     4621 cggcgccctg cgcgtcgccg ccgacctgct ggcccggttc agcagcaaaa ccatctgggt
     4681 cagcaacccg acctgggcca accataacaa cattttcgaa gcggccggtc tgaccgttaa
     4741 atcctacccc tactacaacg ccgtcaccaa agacctcgat gccgacggct tcttcgccac
     4801 gctcgaaaaa attccggccg gcgactgcgt cctgctgcac gcctgctgcc acaacccgag
     4861 cggcgtagac ctctccgccg cccagtggaa acaggtcgca gccatcgcgc agaaaaacgg
     4921 ctggtccgcg ctggttgact ttgcctacca gggcttcggc gaaagcgttg aagccgaccg
     4981 cgtcggcgtc gaagccctgc tggacagcgg cattgaattc ctcatcgcca gctccttctc
     5041 caaaaacttc ggcctctacc gcgaccgcac cggcgcgctg accgccgtta ccgccacgcc
     5101 tgccgacgcg gcgaccgcca tgagccacat gatggccatg gcccgcgtac tctattccaa
     5161 cccgcccgca cacggcggca tgatcgtcac caccatcctg cgtgacaaag aactctgcaa
     5221 actctggcgg agcgaactcg acgccatgcg cgaccgcatc gcccaggcgc gcgcggcgct
     5281 ggcccaggga ctcaccgaac gcggcgtacc gatggactgc tcgttcatga cgaagcagaa
     5341 aggcatgttc tccttcagcg gcttgaacaa agaccaggtt aaattcctgc gcgaagaaaa
     5401 agccgtctac atcgtcggca gcggacgtat caatgtcgcc ggactcaccc ccggcaacat
     5461 gggcttcgtc tgcgacgtcg tcgctgaagc catgaagcgc taaaattcat tttttagctt
     5521 ccgagacaga tgcagtcgct gaagttttgt cgattatctt gtccatcacg accgcaagaa
     5581 actcttggtg atgctcttcg gaaaaagaga acgcgaactc cttttctttg gtgtagaaaa
     5641 agatttcctc gtgtttttta aactgcagca aaaatacaaa cgctgaagct attaaaaaca
     5701 acaagacggc tatgtacaga taatgggaag tcctattggc caatatcgac atgggtgcac
     5761 ttattacaaa aagaaggatg gaacaattgg ctgcgttggt gaccccaact ttcatttttt
     5821 tggtaatagg tgaaagatcc gcatatgaat attcgcgagt aaattcgagt ttcagtgatt
     5881 tgtaatgcca tactagctta tcttccgtgg cacacaacgt actgcgactg aaatagtctg
     5941 tgagacatcg ttcggtaatt ttcatattta ttgcttccct catcatgttt agaggtcgcc
     6001 tgatctaaat ccgctaccaa cggagcaatg ctatcaaagg ccaaggttga gcggcagaag
     6061 aattcctgga tttcggcggg aatccaactc ataaactgag cccgaagagc cggagcttgg
     6121 ctgtcagcaa attgtccagc accggcaaat ctttcggata gacggagacg agtcgcttgc
     6181 cttcctgctg atagagcgtc tgttttttat acatgctcat tttatactgc ggcgtatcca
     6241 gcccccagta ttcgatatag acatccacct ccggcagata aaagtcgggc cggatctgaa
     6301 attcgccgat gacgcgatat ttggcgtcgt agcggtaaga aagtccgtgc tccgtcaacc
     6361 attcagcgac gcgccgctcg ccttctgact gcaccagtgt gccgtcccgc gcttcgatgg
     6421 ttttattgat ctcgacctgc gtctcaaaat tacgccgctc cagaaaaact tcatcaaaac
     6481 aacggtcgca gaacacacgc tgaaaaactt cgttggcccg ggcgtattct tcgggaaaaa
     6541 ccgcggcgtt gcatcgtttg caccggtgtt tcaccccggc accggcatcc tgtgcggcca
     6601 gctcaattgc atctgcggcg gtcttggcgg cggcccgtac atagttcggg ttggccgggt
     6661 tgcgagccaa gtcgcgcaga tccggcacct gcgactgcgt agcagccccg tactttttga
     6721 gtgcgcgaat ggcgtattgc tgtgtctgag ggtgccgtcc gtgtagcgct aacggagcca
     6781 ataccgccac agccttcccg gcatcggcac cgaaatctgc cagcttccca atggcagacg
     6841 ccgctaaacg ctgcacctcg ttcgagggaa ggtgcgacag ttcgataagt tcaggcaatg
     6901 ccgccggatt gccgctgcgt ccgagacgca aagctcgttg cacggcatct tggtgttcgg
     6961 atggcagcat tgggcttttc atgggaacct cttttgtttt ccgtgactcg ctgtcgcttg
     7021 tcacaaaaac tcccatcggc aagcggtagc gagtcatgtg tttctgaaaa gaattttcca
     7081 gagttgaaaa aaattcaatc caaaacgatt tcctgccgat actccggcac cgtgcagctc
     7141 cagccggttt ttccggtcag gaagtttttg aaggcgacgg cggcgtccgg ttcgccgtgg
     7201 gtgatgaaca ctttgcgcgg cgcttttttg agtccggtaa tccaacgcag cagttcgtcg
     7261 cggtcggcgt gggcggagaa gccggagatt ttttcaatcc gtgcgcggag cggctgcatt
     7321 tcgccaaaga tgcggacttc cgcgggctgg tcctgaatcg cgcggcccag cgttccggcg
     7381 gcctgatagc cgacaaacag gatggtgctt tccggacggc tgatattgtt tttgaggtgg
     7441 tgtttaatgc ggccgccggt acacataccg gctccggcga tgatgatggc cgtgcctttg
     7501 atatggttaa tggctttgga ctggtcgacc gtgttcgaca gggtcagccc cggcaggtcg
     7561 cacggatgaa tgcccttacg aagcaggtcg cgggtttctt cgtcgaacag ttccggatgc
     7621 tttttgaaaa tctccgtgac gcggatcgcc atcgggctgt ccacaaaagt gaacagatga
     7681 gggatgcggt tttcgcgcag cagtccattg aggtggtaga gcagctcctg cgtgcgctcg
     7741 acagcgaagc tcggaatgac aatgttgccg ccggctttat gtgtttcgtt gatgatgcgc
     7801 gcaagctcgc ccggaatgtc tttggtttcg ccgtggagac ggtcgccgta ggtggattcg
     7861 accagaacat agtccgcgtc ttcgaactga tccgggtcgt tgatgatcgg catattccag
     7921 cggccgacgt cgccggaaaa aagaatcgac cgcgcctcgc cgccctgccc ggcggtcagt
     7981 ttgatactgg cagaaccgaa gatgtggccc gcttcgcgga acgaggcggt catgccggca
     8041 gcaacagcaa cgggtgtttt gtagccgacc ggcgtgagca gcgggacggt tttttcgaca
     8101 tcggcggcgg tgtagagcgg ttcatagggg aatggactgg tctttccctg tttctcatgg
     8161 cgcgcctttt tgtgtttaat gtcttcttcc tgaaggacag cggaatcttt catgatgatc
     8221 tgcgcgatgt ccgcagtggc ggaagtacag aaaattttcc cgcggaatcc gtctttcacc
     8281 agcttcggca gacgtccgca gtggtcgagg tgggcgtgag tcagcagaac cgcgttaatg
     8341 gttgcgggcg gaaccggaaa ggcgtcccag ttgcgttcct tcagatcgcg ctcctgatag
     8401 agcccgcagt cgaccagtat gcgcgaaccg ccggactcca ccagataaca ggagccggta
     8461 acgttcttcg ccgcgccgaa aaagtttaat tttatgctca taagccggcc tccctttttt
     8521 tcttcgggtc agtgtagtga cttttacaca gacgggcaat taaacccttg tgcgcgggcg
     8581 ggtttttgta ttctctataa acggtgccgg gcatgtggtg aaattggtat acacgcaaga
     8641 tttaggatct tgtgcctcac ggcatgcggg ttcgagtccc gccttgccca ttcaatgcac
     8701 cgaaggcgaa agacagggat catttgaaca aaaaggagat ctgatgctgc cgcaacacgg
     8761 aggagtaccg aatttcagcc agcgcatccc cgccccgatc ttaaccttgt tcatactggc
     8821 gctgattacc ggcctgctgc tgacgctttc gttttacctc actgccgacg aaggtgatct
     8881 ggccgcgcaa agccgcgtta aactttctct gctcgtcacg ctgctggtgt catttttcat
     8941 tctgctgtcc ggcacgggcc gctggtggcg cccgcaccta tggaatcata aaagcgggca
     9001 ccgcccgcac cgcaggcgca gatcccatca ccggcattga ccgtttatgt ccggcggttt
     9061 ccgggtaaag acggaatgaa ccgcatttca aaagccttca atgccggatc gtgagcttcc
     9121 ggaacgggct gaaattatca gatatcccgc tgccagtccg gttttccctc gtaaacccag
     9181 ctgtaataat ctttatgctt caggcctgcc gccgccgctt cgtcgatgaa tatcttggcg
     9241 caggcatgat gctgaagaat cgaggccggc ataatggccg cgaccggacc ttccgtcatt
     9301 gcagccaccg ccgcggcttt gctttctccg aatgccagca gcagattcat ccgggcttcc
     9361 atgatggttc cgattcccat ggtaatgcag tgatgcggaa cgttcgattc gttaccgccg
     9421 aagaagcggg cattgtcggc caccgtctgg cgtgtcagcg ttttgatgcg ggtgcgcgat
     9481 gcgaacgagg atgtcggttc gttgaatccc acatgcccgt cagagccgat tcccagaatc
     9541 tggatatcaa ttccgcccgc ctttacaatc gccttttcat aagcggcgca atacgccgga
     9601 atatctttcg tcattccgtc cggaatatga atgttcttcg gtttgatgtt gatgtggttg
     9661 aacagatttt cgtgcatgaa atagtgatag gactgcggat gatctgccgg cagccccaca
     9721 tattcatcca gattgaacgt ggtgacactg ctgaaatcca gtccttcttc gcggtgcatc
     9781 cggatcagct ctttgtaaag cagcaaaggc gtactgccgg tcgccagtcc cagaacggtc
     9841 tccggttttt cacgtatctt tcgcgcaaca actctggccg cagcccggct gccggataag
     9901 gcgtcttttt gtatgataat ttccatctcg ggtctcctgt gcaggtgatt taagccgctc
     9961 ttaaaacaga gttttcagat ccgtttccat cgaaatcatg cggccgttgt taaaaacgtc
    10021 gttcagcccg ccggcgcacc accggcgcag acgataaagc gtggcaaaag gaaaatcggg
    10081 agccgctccg ccctgcccgc cgaccagctc gccgccgcgc cgcacattat cctgcatcca
    10141 ggccggcatt gtcaggtgat agggattgcg ctccacttct ttgacatgaa aagaagtcgc
    10201 ggcaatcaga tcggcaacgg tctgcgcgtc cgcttccacc atcagattgg gatgctccat
    10261 cgcctgccag tattctgttt cgacaaccgt gcaggaaaaa tccggaggca tggccgccag
    10321 cgcttccatg acaatgaaat gcgtggcaat gtggcgggag ttccagtctt tggaatgcgg
    10381 cacgaaaatc accgccggct gaacggtttt gagaatttca actacatcgg atttctccaa
    10441 attcaggaat ccgctgcggc gctgaatatc ccagccgaga aaacggcagg cgttttcaag
    10501 ttcctcagcg cggccgggct ggcgcgcgga attgcttccg aacgtaaccg gaacattgat
    10561 aatccgcatg cctgcttcgc gcataagccg caaaggaagc gtgccggtaa tacactcgtc
    10621 atccggatgc ggagaaaaca gcagaacaca cggagcatcc ggcggaagcg cgggccgcag
    10681 acagggcggc attccgccgc acggcatcgt gccggctttt ttcaccagag attccatctg
    10741 ccgggtatat tccaagtaag gattattcat gctcatctcc atttaaatct gcggcagact
    10801 cgctgccgct acggcctgac ccacacgccg gctctgttca tccggaacat gcagccgggc
    10861 ggaaaaatca gggaattctt ttgcgagcac tgcgcgggcg gtttcgatta ccttttcgcc
    10921 gccgcgcccc gaagtgaccc gtccgagaat cagcacatct tcgaagtcat aaaaccgcgc
    10981 ataccacgga agcgcgtagc cgagatagac tccgatggtt tcaaaaatct tcagcgccgc
    11041 ttcgtttccg gcatccgctt caacctgcac ggctttcaac cgttccggca gaccggtttc
    11101 cgccgggaaa ctgaatcctg ccgccggagc cagtttattg accgcctgct gagaaaaata
    11161 aagcgctccg accccgcggt cgccggacca ttcatccgca gcggcggccg gattaaaatc
    11221 aaccggagca aaagccagtt cgttaagccg gccggttacg tgcccggccc ggtccagaaa
    11281 cccgacggct tcgctggagc ccatggcgat tccgaacatg ccctcttttt tcagggacat
    11341 ggcaccggcc agagcggtaa catcgccgtc attaataaca gtcagcggca caccccactc
    11401 ctcgcgcagc cgatgaaaca tattttttat tttggtttcg tacagcccgg ccggaaccga
    11461 ccggaaaagc gaagccacca tcggacggtc atcaacaaaa acccccgctg aactgccgcc
    11521 gatggcatct acacgcggca aatgctccgc cgccagcttc agtccctttt ggatggcctg
    11581 ataatgatag tccggatcag cctgtttgac cggatcccat ggaatctcgg tgctgaaaac
    11641 cggctgtccg tctttgacgg cggcaagctt gtagtcgctc gcgccgagat caaagccgat
    11701 acggcatccg gcgagatgtc cgccgattgc ttcttcgttt tctttgtccg ccgggatttt
    11761 tccgggggca cagcagactg tttcaaacgg gcggtcataa acccgttcca ttaacgccac
    11821 gtcaaaagcg cgggggccgg acgacgaata ggttttcgcc atttcccggc cgatccattc
    11881 tgccccgccg atatacagtt tccagccgcc gctggcccac agcataaatt ttacaacccg
    11941 gtcgacatac agcagagttt cagtccgcag cccggacgat tcggttgcga caagcagctt
    12001 tttgcggctg acgcagccgt tttcccgctc tacggctatg atcagttctt cggttttttc
    12061 cagcttctgt aagtagtccc gctcaaacag gatcgccggt aaaaactccg gatcaagggg
    12121 tgtcggaact gccagacggc tttttttgag ttgtgtgctg ttcatgttca ttgtttgcct
    12181 cggtaatttt tgaattacaa tttctatatc attttcattt gagcgatcaa aaccattaat
    12241 tagaaagata aatcccggta cgaaccaaac gattttcaaa cttcaggata aaaaaagagc
    12301 cgtctggcaa acaggtaagc gccggtctgc atggccagcg ccgcatcctc gctggctgcc
    12361 ggatcgagcg ccgcaatttc ttcatcttca aatgtggagg caagcccttc cagcaacgca
    12421 gaccgtaccg ccggaaaaac ggaagaaagc gggccgctga tgataagccg ttcgctctgc
    12481 ataatttgca agggccagca gaggctgctg cccagcagcc gggcagcatc acaagccgct
    12541 ttcaccgcgt ccgggcatcc cgacaaaacc gcgctgcgca gctcttccag acttttaatt
    12601 tttttatcct gcagcgcttc gatcatggcc cagccgccgg aatacgcctc gagacatccg
    12661 cgccgtccgc aacggcacgg ccggccgtta cgccgaaccc ggatatgtcc gatttcggag
    12721 agcagcgagt cgccggtatg tgaaatgatg cgctcaccga ctcccgcaac agccccgacc
    12781 cccagatccc agaacacaac cattgtccgg gtgtcggcgg ccagccgccg cgcctctccg
    12841 taataaaaag ccagtccgag cgaaccgcag aaacagggca gatcatagtg ctcctgcatc
    12901 caccggcgga aatcgatgcc gttcagcgca ggaaaattag cggcatttct gattacgctg
    12961 cttttcggtt caaacaggcc gggcacaccg gcaaaggcct gccggatgac tccgccgata
    13021 cgctgcgcct tgaccacggc ggcgttaaca aagttctcca cttccgctgt cagcgcccgg
    13081 ctgtccgcaa tgcctgtgag atcctgccgt ttctgaagca ctacatttcc tttgaaatcc
    13141 accagtgtgg cctgaaaaaa cggaacatcg aaaaccagca gcagacagag attcttatcc
    13201 tgttccagct cccagacgat agtggcgcgt ccgcggccgc cgccccggat tgaatctgca
    13261 cggcggatca gccccatgtg ctcaagcttc tggaaatgca ggttgccggt tccggaggac
    13321 agcccgacgg cttccgccgc ctgcacctgc gtcaccgccc cctgcctgtc aatgagctgg
    13381 aggatacttc gtgaactgct caaatcctca aaagacagcc ggctgcggat gtaggatgga
    13441 agatctctgc gcgccatgaa cgctccattt aatttgatga tccatattaa ttcatcaacc
    13501 aaagcctgaa agcaacgctt aacttgcgaa ccggcgccgc cggaacagtt aatcagcaga
    13561 aaactgcttt aaaaagcccc cggcagcagg gtcgtgtgca acgccgaacc gggacgctgc
    13621 ggcaggacga atgtacgcgg catctaactt acaatcgcca ctttgttcag agcggagctt
    13681 ttttgttgcg ttgccgttag tcacaaagta acttctccgc tctcttttca atggacgcgt
    13741 agctcagtcg gtagagcagc tgactcttaa tcagcgggtc ctcggttcga gcccgagcgc
    13801 gtctaccacc tctaaacact cagacgcata aacttcagca ggttcagact ggatccgaag
    13861 cccgaatcca accaggggaa atagccggtc agattgaagg ctgttaaatc gacgaggcat
    13921 cagaaacggt ttcaccggct cgataagcgc gcggcgagag gcccatgatt ttgcggaaca
    13981 gccgcgaaaa ataataggag tcggcgaagc cgagggtttc gctgatttcg ctgatccgca
    14041 ggctggtggt agtgagcagt tcgcaggcct tttggatttt caggcggctg aaataatcca
    14101 tcggcgtgtg ttcataacgc cggttgaaga gcgcgccgaa atggttcggg ctcataccgg
    14161 aaattttcgc cagctccttc aggctgagtt ttttgctcag attgcggtgc ataaactcca
    14221 cggtcttatc gaggctttcc tcagccgtgc ggcatttgcc ttccgaggcg agcatgcgca
    14281 gctggatgag tccgagcagc tggtttaatg ctccggacgc cgcaaccagc gtggaggacg
    14341 tgtgcacctg gcccatgtat tcatacagct gctcgatggc ggcgatgact tcctgccggg
    14401 cggaaagatg gatcaccggc gattcgccgg ggcgcgtgat cagccgggcg aagtcggccg
    14461 cctgccggcc ctggaaatga acccagtaat tcgaccacgg gtcgtcgggg tcggccccgt
    14521 aggtgtgcgg aatgtgctga ggaatcagga aggcttcgtt ccgggtgacg tcccatgact
    14581 gtccgttgat ctccagccag ccgcggcctt ccgtgcagaa aatcaagatg cagtcatagc
    14641 ttcccagcgg gcggggaata taatgggcgg cggacggccc ctgaaagccg acccgcgtac
    14701 acagcagatc ctgcaggatc ggcgcaacca gcgcctcgtc gcgcagaccc ggagccagat
    14761 gggccatccg catatccgga aaggcgccgc gctgtgcgtg acggatggtg cgcagcggaa
    14821 cgtccgacag attatgaact gtcagcccgg cttttttgag cagccgtagc acttcttttt
    14881 cgtttttccg gcacatggaa ccctcatcag ttgagggcta taatccatta ttccattttg
    14941 ttgtcacgcc atttgaatgg ccgtcatgcc ttttggcggc ctcgtttacg ggaaggtgcc
    15001 ggagttcagg agccggaagt tttagggatt ttatccatct tctctgtgaa gttgtccatg
    15061 gagaacatgc ggggcttcgg gctatgctgg tgaaatatta aaaaggggaa acgagtcatg
    15121 gatgttacga aaacctgctg tcaaaccgcc gccggcggcg caaaaaccga acgtgtgtat
    15181 gcggccgatc tgcaccagag ccgcttcaaa ccgaatgacc ggctgaaaaa gctggttgac
    15241 cggcgggact ggctggcaaa aaaccataag cgcgacatct attgcgataa cgccgagttc
    15301 ctcatctacc actggtccgg caaggccgag acgtttgccg cggcaaccgg cgagcaggcc
    15361 gcgtccgaag aggagcggat taatatcggc gaccacacct gccgcgtcga tgtcggcgga
    15421 ccctttccgt atgccgcgca aatgccgtcg aaggtgaacg gctatgagcc gaccgtcacc
    15481 aactgggccg cggactatgc ctttttcctg cgccacagcg aggccgaggt gttcccttac
    15541 gaacagattg tcggcgagtt ccactggcag ctcgacgaag cgcggtttta taaatatccc
    15601 aagtcccacg ccgatctcgg gctcaaagcc cgcgagctcg gtgccggcgg attcagtctg
    15661 gcgcactgct gccccgatct ttccatcggg ctctcgctcg gctggggcgg cctgctggag
    15721 aaggtgcgcg ccagtcgcga aaaacaccgc aaattcggca acgaaaaggc cgtgacctat
    15781 ctggacgcca gcgaacaggt ggtgctctcg gtgatcgatt atgtgcagcg gcacagcgaa
    15841 aaagcggccc ggctggcggc ggtggaaacc gatccggtca aaaaaaccag ttatgaaatg
    15901 gtggccgaaa actgcgcggc gattgcgcgc ggcgcaccgg caacctacca ccaggcgctg
    15961 cagtggattt tcctctacat gatggtcgag cggatcaacg gccatggcaa cggctacggc
    16021 cgctttgatc agctgctgat gccgttctat gaaaaagaca aagccgccgg acgcatcgac
    16081 cgcaaccgcg cccgcgagct gatggccgag tggtacctga aatacggccc gcacctttcg
    16141 ttcggcggac gcctcgaaga ccgctccgac gccaccaatg agatgagctg gatcggcctc
    16201 gaagcctacg acatggtcgg cggctacaac cagttcggcg tgcagtggca cgcggatatg
    16261 gatccggaat tcttcaaata ctgctgcgat gtggtcggcc ggcacggctg cggcgttccc
    16321 gcgctgctca attccgatgt gatggtggcc agtcagctgc gcacgggctt tgaggaaaag
    16381 cacgcgctga atacctccta cagcggctgt cagtggtact gcattcccgg ccgcgaatat
    16441 caggaccagg atgtgaactg catcgtgctc atcagcccga tgcagcgcgc gatcgatatg
    16501 gccattgagc agaaggtcag cgactggaac ggcttctggg ccatctactg ccgcgaagtc
    16561 gaccgctccg gcgaggcgct ggtggccttc aagaacgagg cctacaaata tcacgcccag
    16621 ctctggccgg aaatgatcac ctcgctcgtc tcctacgggc cgatcgaaaa gggccgcgac
    16681 atcaccgact tcgacgccgt ggattacaac tacccctccg tcaacgtgct gggcgtgccg
    16741 aacgtggtgg attccatgta cgcgctgaag cgagcggttt tcgaagaaaa acgcttcacc
    16801 ctccaacagg tgcgcgacgc ctgcgcccgc gactggagcg gcgacggcga agaggccatg
    16861 cgccagtatc tgcggaacct gcccaaattc ggcaacgata tcgatgacgt cgatgccatg
    16921 gcggtgcaga tttccgagca ggtgcgcgaa acggtggaat cgaagcgctg cctgaagggc
    16981 ggccacttcc gcgcctcgct gttccagtat cagggccaca ccgtggccgg gccttatctc
    17041 ggcgccacgc cggacggacg caagcgccgc gaaccgctgg cgcacggcat gaacccgatg
    17101 cacggacgca ataccaacgg actgagcgcc acggcgaatt cgttctgcaa gctcgacttc
    17161 agcaaatacc agggcggctc gttccagatc gaactgcacc ccagcttttt tgcggagggg
    17221 tttgatcgcg ggcagctggt ggatattttc gcgcgggcct tcttcaaaaa aggcggcgtg
    17281 cagatcaatc tgaatgtctt tgacctcgac gatctgaagg aagcctatga gcatccggaa
    17341 gacgaaaagt acgaagacat cgtggttaaa gtgaccggct actccgcgca cttcaccaag
    17401 atggaccgcc tgttccagaa agagttcatc gagagggtta actacgagtc gctgtgatcc
    17461 agcgtccggt ccggccggct acacctgcaa cgtgctccgg ctgcaaaccc acgacgggcc
    17521 ggggctgcgg accaccgtct tcatgaaggg ctgctcgctc cggtgcgcct ggtgccataa
    17581 cccggaaagc ctttccggag cgcagcaagt ctggcatttc ccgtccaagt gtatcggctg
    17641 cggggcctgt cagcaggcct gcccgtccgg cgcgattgcc gtccggccgg gcggtctgga
    17701 aattctgcgg gatcgctgca ccgggtgcgc ccggtgcgtg gcggtttgcc cggcccgggc
    17761 gatggaggcg ctgcgcaccg aatggactcc ggactcgctg atggatctga tccgccgcga
    17821 tctggcgttt ttgagtgaag gcggcggcgt caccttcagc ggcggggagc cggcgctgca
    17881 ggcgcccttt gtggcggagg ttttgaagcg gtgccgcgcg gcggggctgc acacggcgct
    17941 cgatacctgc ggggcggtgg accggtcggc ctacgatcag ttgctgccgc actgcaattt
    18001 agtgcttttc gatctgaagc tggccgattc cgaggcgcat cagcgctgga ccggccagcc
    18061 gaacgggcgg attctggaga atctgcggca cgtggccgag acggcggggc agcggccgga
    18121 tctggagctg tgggtccgga cgccgctgat tcccggcgct acggcgactc cggaaaatgt
    18181 gggcgcggcg gcccggctgc tgcgcgactt gccggccggt tctgtgagcc gctgggagtt
    18241 atgttctttt aataatcttt gtgcggataa atattcccgg cttggctcgc cctggtgctt
    18301 taccggtgag ccgcttcttc aacggagttg tgtgtcggcg ttgcggcagc aggcggcggc
    18361 tgagagtgga ctgcaggaaa cacaggtaca tctccgcggg cgctggcaag acggctgacg
    18421 gcagaaacgc cgggtgcaac ccttgggttc tgtcttcagc cgccgcaccg cgaaaattga
    18481 aaccattatt cgccggaacg caaggcggcc tcttccgttc gatgggttat tcggccttca
    18541 gtatccatcc ggcatatgaa attccgggca caacgaagag gtcgccggtt tgtttcattt
    18601 catcagccag ctgctgacgg tgctgctgca gaacggatgc atagtccggg tcgagcgcca
    18661 ggttgtgcat ttcgccggga tctttgttca tgtcaatcag ctgttcgcgg atgcggccgg
    18721 cggaaaacac gcagtatttg taatcctttg tgcgcagcat gcggccttcc ggtcccggca
    18781 gattgtcgcg cggcgagagt acggtttccg agaccagccg ggtgcgccac ggcgcggcgc
    18841 ggccttccgc cagcgggcgc agactcaagc cgttcaggtc gccgggccgg ttggcgccgg
    18901 cgtaatcgca gaaggtcggg aagaggtcga ggccggtgga gatcaggtgc tcggtatcga
    18961 ccgtgccggg ctttttcacg ccgggaccgc tgagaatgag cggcacgcgg gcggtttctt
    19021 catagaaaag cgatttctgg ttccattggt gagcgccgtt gccgtcgccg tggtcgctgg
    19081 tcagaaggat taacgtgttt ttgtcgagac cttcggcgcg cagcgcctgc aggattttgc
    19141 cgatttccga gtcaacgcgc tcggtgagcc ggttcagcgc ccagcggtat tgccgccacc
    19201 gttcatccgg ccagccgatc acgggatagg cgctcttttg ggtggcctgg tattcccgga
    19261 taatgtcggg ttcgttggcg ggaataccga aattcgccgg tagagccggg cagagttcag
    19321 gccgcggggt ggcggcgatt tctccgttcc acaattcggt ccggctcgcc ggaaagccgc
    19381 cggtcgcccg gcgggcccat tcgcaaatat cgtgcgggtt gacgaaggag gtgaccagga
    19441 agaaaggctg gtcgcgtttc tttttaatga aatcgatggc cggttgcgca aagtgctggt
    19501 cgttgaacgc cttggagcct gccatcatca gattaaagcc gtgccagctg ttgctccggg
    19561 tctcggtcgg catgggaata tgccacttgc cgatgtatcc ggtgtcgtag cccgcggcgg
    19621 tcatcagttt tccgatcgac ggagcggcga catcgtaacg ttcgatgttg gtcatcacgc
    19681 cggtctggtg cgacatccgt ccggtcatca tcgctgtgcg tgccggcacg cagatggggt
    19741 ttacgctgta cgcgcgctca aaccggatgc cagcggccga cagactgtcc atggcggggg
    19801 ttttcaggtc cggattgccc gcgcagctca tggccgtcgc gggctgctga tcgaccatga
    19861 tccacagaat gttcggacgg agatccgctt gcgaggctcc gccggcaagc cctgctgtaa
    19921 acgccagaga agaagagatc aaatgcgcac attttttcac gcgaaccgcc tttcgtaatt
    19981 gcgtccgaag ttagttgttt gaatgccgca ctgcaaatct ttgaggcggc aatttcgacc
    20041 ggggaattta cggtaaaact gcaaaataga gaaagagaaa gcttccgcat ttttgggcga
    20101 tcccttcgtt tcgaacgact gtattccggc ggatcaaccc ggcagcgacc agcacaccgc
    20161 cacctgccgg cgctccatac aaaaaggtta aaagatcgga gcgtcccctt cgcccttcgc
    20221 cggccaggtt aaggctttga ctccggctcc tgagcgggct ttattttctt cagcggaatt
    20281 tgaacattaa acgtccctcc gtcccgtttc ggattggcgt cattaatgga acaggccgca
    20341 tacaatgcca gcgggtcatc attctcgtcc aggagcagct gcggccgctc cagacgagcg
    20401 acctttaagc gggtcccgtc cttcagaatc agctctttct tcataaaaag aggagacggt
    20461 gcaagcgtcc agtccattcc gtcgacggat tgcatcaggg ccagtccggg ccctccgcgg
    20521 gtaagttttc ctgtaaaatc ttttatgacg gcgtaaaaga gtttgtcttt atggtgatac
    20581 cacacatacg ggtcttcggc tgaaaccttg tggccttttc cgtcgtcgac gtcgaagacg
    20641 aaccggtcgg ttgtcgtaaa cggaccggtc ggcgaatccc cgacggccac tccgtgaacg
    20701 cccctccagt tcgggtcgta gatgtttcct ttaaagaaca gtaagtattt tccgtcggca
    20761 gggcggtatt ccacggccgg gtttaccgca atcagattgt cggggccggc aatggttccg
    20821 ggaggagagg ggtcaaccac cgctttgctt ttcactcttg ttctcggaga gaccagcggc
    20881 tgatcgaatc gggtaaaccg cccggccaga agatcctcga cggaattgaa ctcgatcaca
    20941 ccgatttgct gactctgctg gacgcggttg cgttttgtta cattttcacc cggtgaacct
    21001 ttcggctgga ttccggggtc cttgccggcg cagtaataca ggtaatattt gccgttgaaa
    21061 tacttgatca gcggattatg caccatttgc gcgtcccagg ccgttggatc gccctgatct
    21121 ttgcgtcctt tcaaaaccag ccccagtgag cgatagccgc cgtacggaga atcggaaacg
    21181 gccaatccga ctttggagaa cagcacccag ctgtccgcaa acggcgggat cgaagggcct
    21241 gattcccacg tcgaatagaa catgaaatac tgatccttga tccggatcgg gctgccgcac
    21301 catacaaagt cgccgggcaa ctgaaaaaca gaggtccctt ccacgcggat cttcccgttg
    21361 tcctgaatca ctttcaggtc gaccggttgc gcctcatagc ggcaggaggt gctttcgccg
    21421 actccggcaa cgctcatcga taagccgcat aaaagaacgc accagttcag cttgttcatt
    21481 ctgtgtctcc tcgccgaaaa aggttcgccg tcctcagcct ctcagccgtc tttatacttt
    21541 caattcaatc tgcggatttt gtggtccgca gacttctgca ccgcgcttcg caggcgctcc
    21601 atcaaaaagc cttggctcag tggtccttct gcggcttctc ggatgcgtcc gccttccaga
    21661 tgatcacccg cgtatcgttg gtctgcggcg cgcccggcgt gctgcggccg ctgtcgatat
    21721 ccttctggag ctgggcgagc atcttcttca cctgttccgg ttcctgtgcg tagcggttgg
    21781 ttttttcgcc gggatcgttc ttcatgtcgt acagctgctg catgggcagg ccgtctttcg
    21841 cggcgtctgc atcgcggagg gtccagccgc cggagccggg gcacagtacg agtttccagc
    21901 gtccgtcgcg aatagcaaat tggccgttga tggagtggtg tatgacatgg tcgcgaaccg
    21961 gcgaggccgg attccggaac agcggcagca ggctgacgct gtcttcgccg gccgttgccg
    22021 gcagcgcggc gcccaggatg tcggcgcagg tggccatcag gtcggagagg cagaccaatt
    22081 cgtcggagcg gcttccgggc gcaatcacac ccggccagcg ggcaatgaag ggcacgcgat
    22141 gtccgccgtc ccagatgtcg gatttatagc cgcggtaggg cccgctggaa aagtgaccct
    22201 gaggttccat ggctttatga aagccggctg cgtcgacgta ggtgccgatg tagtgggcgc
    22261 atccattatc gctgctgaaa atgatcagcg tattgtcttc cacgccgtag cgtttgagcg
    22321 agtcgaggat gcgcccgaac acgtcgtccg tctcaatgac gaaatccgcg taaaggttgt
    22381 tgaggccgct tttgccgatg aacgatttgt tcacggagat cggggtgtgc ggcgaggtca
    22441 gggccatgta gaggaagaac ggttccttgg ctgcggcccg tgctttaatg taatcatccg
    22501 ccttctgagc ccaggtcggc agaagctgtt cgaaattcca atagggcatg gccgggccga
    22561 tcgggccggc ctgattgttt cccagcagcc gttcggacag ccaggtggac gggatgccga
    22621 ccgtgcggtc gttttcgatg aagcagtacg gcggccagtt ggggacgtcg acgccgaagt
    22681 aatagtcaaa cccgcgcgtg gtcgggccgc cggtggtcgg ttttgaaaac gccgcctgcc
    22741 acttggcctg cagctccggg gtaacgggcg ggttcttgcc gttcttcgac aggaagactc
    22801 catcggcggc atcaaaatcc cagcccattc ccaggtgcca cttgccgatg caggcgctgt
    22861 ggtagccata ttgtttcaag aggccgggaa ccgtcagccg gtccttggcg atcagcgggt
    22921 tgccgtaaac tggaacaatg cccttctgca gccgtgagcg ccagttatag cgtccggtca
    22981 gcacgctgta gcgcgacggc gagcaaacgg ccgagctgga gtgagcatcc gtgaaaatca
    23041 tcccttcccg ggacagccgg tcgagattcg gagttttgat ttttccggcg ggattcaggc
    23101 aggacagatc gccatagccc aggtcgtccg ccagaacgaa caccacattg ggtttgccgg
    23161 ccgccagcgc gtcgccggcc tgagtcaagc ctgccgaaac agccaggccc agcatcaaac
    23221 gtgaggttaa cggaagcctg atcgattcat tttttcgttt cacggtttgt ttccttattc
    23281 gggttgattg accgctatag gtcaagattt gagcgaagtc aactcctcgg ccccccgcca
    23341 tcggaaaaaa ggggtccccc aattagaggt tccggtcagc ccttgctcat tttccggtac
    23401 tgccgcggcg acagccccat gagtttgcgg aacgatctgg aaaaataata gggatcttca
    23461 tagcccgtct taaaccctat ctcccgaatc ggaagcgcag tggtttcgag cagccagcag
    23521 gcatgtttca ggcgcagatg gatgaaataa tcgcccggag agagtccggt ttggcggcgg
    23581 aacagggcgg aaaaatggac gacggacatc ccggcgtgct gggccatttc cggaaccgtc
    23641 agcgcctggc cgatccgttg ggtcatcagc gccataatgt tttccagatt gtgcccggac
    23701 cccgtcttgc cgtccggtga atacgccggg ttgttgtaga acagcaggcc cagcagatcg
    23761 cgcaaggcgt gactcaggta gagcagattc cggtcggcat atccgccggc cagcgcgtcg
    23821 tagcaccggc agaaaaccga taccatcgcg gagacgcact ctgcgctcac cgagatgcaa
    23881 aaactgctgg ccggcatccg ctgcaaatac agcggcgcgt cttcgccgtc aaaatgcacc
    23941 cagtggacag accatggagc gtgcggcgaa gcaccgtacg cgtgaggtat tccggcagga
    24001 atcagaaccg cctgcccggc cgaaaccgtt tcgcggcgca gagccccttc gacccatccg
    24061 gcgccgtcga cacaaaggat cagaatgtgc tgccccgcac cgtgagggcg ccggatcgag
    24121 tgatttttcg cgtccggaaa ccagccgatg tcggttacgc ggaggtcccg gaccagttta
    24181 tgcgactcca gcaggctcag aatgctgcgg gaaatcacat ggagaatctg tccccggaat
    24241 ccggatagat gcttctgatg atccattttc atgatgccaa cacctgatta ttaaaaaccg
    24301 cggcagtaat ctgctgcatc gaatagtcca ttttttttat acgatcgtcc attccgtttt
    24361 gccggttccg gtgtaaaact ctaacctgat ttgcaaagcc caaaacgatc atccacggag
    24421 aatcaattat gattacagga tcggcagctg tttcatcgtc cggcacaaaa gcggattaca
    24481 acctcggtta tgtctggctg atttccctgg tggcggccat gggagggctg ctgttcgggt
    24541 acgactgggt ggtcatcagc ggcgcggata ttttctacga gaagtttttc aatctggttt
    24601 cggcgtcgga aatcggctgg gccaaaagct gcgcactggt cggctgtctg ctgggcgccg
    24661 tcgcgtcggg cgtattaagc gaccggttcg gacgcaaacg gctgctggtt ctttcggcgg
    24721 ttctcttcac cgtctcgtcg ctgggcaccg gactggcgaa tacctacgat accttcgtcg
    24781 catggcgcat tatcggcggg gtggccatcg gactcgcctc caatctgtcg ccgatgtaca
    24841 tcgcggaaat cgcgccggcg gcgatccgcg gcaagctggt ttccatcaac cagctcacca
    24901 tcgtgatcgg cattctgctg gcgcaggtgg cgaactggct gattgctcag cctgttccgc
    24961 ccgacgcctc cgcgcaggaa atcctgaatt cctggaacgg tcagcaggga tggcgctgga
    25021 tgttcgccgc cacggcgatc ccgtcgctgc tgtttttcct gggcatgttt tttgttccgg
    25081 aaagcccgcg ctggctggcc aaaaagggac agcgcagccg ggcggtgagt gtcttgtcgc
    25141 gcatcaacgg aacggcgggc gcagaacagg ccatcaccga cattgacgcc acgctggcca
    25201 acgaaaccgg gcggatagat ttccgcgaac tgctccagcc gaagctgcgc aaagttctgc
    25261 ttctagggat tgcgctggcc gtcctgcagc agtggtgcgg cattaatgtg attttctatt
    25321 acaccaaaga tgtcttcgcc gccgccggct acaaggtgtc ggccatgctg ctgaacatca
    25381 tcattatcgg actggccaac ctcgtcttca ccgtcgtggc gatccagagt gttgagcggc
    25441 tgggccggag gttcctgatg ctggccggct gggccgggct gacggtcatt tatctgctgc
    25501 tgggcggaag ttatttcttc cacctgcccg gtctgattcc cgtcatcctc gtgatcgccg
    25561 ccatcggctg ctatgcctgt acgctggcgc cgatgacctg ggtggtgctg tccgagattt
    25621 tccccaaccg cattcgcggc accgcaatgt ccatcgccgt ctttgcgctg tggacgggct
    25681 gcttcacgct gacgtacacc tttccggcac tgaacgcaca gctgggatcg tccggaacct
    25741 tctggctcta tgccggaatt tgcgccgccg gttatttctt ctgccgggcc cggctgacgg
    25801 aaaccaaagg caagacgctg gagcagatcg agcgcgagct ggtggattaa actgcgaacc
    25861 gatcggtatc gcatccgaag gcggccccgg tcgggcgcct gaaaacagtc tgcaatgaat
    25921 gcaggccgga gccgttgcgt cggctcaaca caagattatg aaggagaaaa acatgacgtc
    25981 acgcgaacga atcgaagcaa cctgcgccca caaacaaccg gataaactcg ccgtggattt
    26041 cggcggaggg ttccagaccg gtatccacgt cagtatggtc tatcagctcc ggcaggcgct
    26101 cggcctcgac aagcccggca cgccggtcaa ggtcgtcgag atttaccaga tgctcggcga
    26161 gattgcgccg gacctgcagg atgcgctcgg catcgatacc gtgtcgctgt gcggaaccgg
    26221 cacgatgttc ggctttccgg cgaaggagat gaaggaatgg cgcatgaacg acggcacgcc
    26281 ggtgctggtg cccgtcgatt tcaatacggt gctggaaccc aacggcgacc tgcttcaatg
    26341 gccggagaac gaccgctctg tgaagcccag cggacgcatg ccggcgggcg ggtatttttt
    26401 tgacgccatc attcggcagc agccgattga tgaagacaat ctcaatccgg ccgataacac
    26461 cgaagagttt tctcccattg ccgactccga gctcgatcat tatgcgcgcc gggcgatctc
    26521 gctttatgag caaaccgacc gcgccctctt ctgcaccttc ggcgggctga cgttcggcga
    26581 tgtggcgctg gtcccggcga ccttcctgaa aaatcccaaa ggcatacggg atgtcgaaga
    26641 gtggtatatc accaccgcct cgcagccgga ttatgtgaaa gcggtttttg aacggcaggc
    26701 tgaagtcgcc atcgaaaatc tggcccggct tcatcaggcc gtcggcaacc ggatcagcgt
    26761 cattcagacc aacggtacgg atttcggcac ccaaaacggc ccgttctgtt cggtggaaac
    26821 ctatcgcgag ctgtttctgc cgtaccagaa aaaggtcaac ggctggattc acgccaacac
    26881 gaattggaaa acctttatgc attcctgcgg cggcgtatgg ccgctgctgg acgaaatcgt
    26941 cgaggcggaa ttcgacatcc tcaatcccgt gcagtgctcg gccaccggca tggatccccg
    27001 gaagctcaag aaaaactacg gcgacaagct ggtgttctgg ggcggcggaa tcgatacgca
    27061 gcacaccctg ccgttcggaa cgccggacga agtccgggcg caggtccgcg agcgggttga
    27121 aattttcaat gaaggcggcg ggttcgtttt ctgttcgatc cacaacgtac aggcctgcac
    27181 cccggttgcc aacctgctgg cgatgttcga cgcgatgcgc aatccgtaag ccgtatcgga
    27241 ataaaggaat cacacatgcg gaacacaaga ttcgctgcct ggctctcgtc gattgttctg
    27301 ggggccgcgc tggcgcaggc ggcagactcc gtatccctgg ccggcgaatg gcgctttgcg
    27361 ctcgatcgga aggatgccgg cgtggcggaa aagtggttca gcaagaatct cgccgaccgg
    27421 attcagctgc cgggcgcgct gcagaaccag ggtttcggcg acgacatcac cgtggcgacc
    27481 aagtggaccg gcggaacccg cgtggagcgc tggctcacct tgccgcagta cgaaaagtac
    27541 cgccagcccg gcaacatcaa gattcccttc tttctgcagc cggacaagca ctacaccggt
    27601 gcggcctggt atcagcgcga tatcgaaatc ccggcgggct ggcagggccg gcgggttgtg
    27661 ctgacgctgg agcgggcgca ctggaccacc caggcgtggc tggatgaccg gccgctcggg
    27721 tcgaacgtca gcctttcggt gccgcatgtc tacgacctgg gcaccggcct cgcgccgggg
    27781 cgtcaccggc tgtcgatccg cgtggataac cggttgctgg tggatgtcgg cgcccgggcc
    27841 cacagcgtat cggacgaaac gcagggcaac tggaacgggc tgatcggcaa cctgacgctc
    27901 tcgtcaaccg gcccggtgtg gattgacgac gttcaggcct acccggacat cgccaaaaaa
    27961 accgcgcggg tgaacgtgcg tttcggcaac gccagcggca gggccggttc gggcgttctg
    28021 aaaatcggct ccatcagccg gccggtcagc tggctggaaa acggcggctc ggcggaagtc
    28081 gaagttgcgc tcggcggcga cgccggactt tgggatgaat tttctccggt gctgcaaaag
    28141 ctgaatgtgc agttgagcgg ggacggcgca atggacgagc gccagctggt tttcggcttg
    28201 cgcgagatcg gcaccaggga ccgccagttt gtaatcaacg gccgcccgac ctttttccgc
    28261 ggaacgctgg agtgctgcat tttcccgctg accggttatc cgccgacgga tgtcgaagcg
    28321 tggaagcgca tcatccgcat ctgtcaggat cacggcctca accacatccg gtttcattcg
    28381 tggtgcccgc ccgaagcggc ctttgtcgcc gccgacgaac tcgggtttta ttaccaggtc
    28441 gaagtggccg cctgggccac cgtcggcgac ggcgccccga tcgatcagtg gctgtatgat
    28501 gaagccgcgc ggattctgaa agcgtacgga aatcatccgt cgttcctgct catgccgtac
    28561 ggcaacgagc cccaaggcaa gaaccgggag cgctggctcg gcgactgggt gaaccactgg
    28621 aagcagaccg atccgcgccg gctttacacc agtggcgcag gctggccgtg cattccggaa
    28681 aatcagtatc acatcaacga caacatccgc ggctccaaag gctggctggg tcgcgattac
    28741 cagaaaatgc tggatgccgt gagcctcaaa aacgtcaccg tgaaaaccat cgatgtaccg
    28801 atgatcgctc atgaagtcgg ccaatactgc gcctatccgg attttgacga gatgaaaaaa
    28861 tacaccgggc cgctcaagcc gaaaaatttt gaaatcttcc gcgaatcgct cgccgaacac
    28921 ggcatgctgc atcaggcgaa agagttcgtc atggcctccg gaaaacttca ggcgctttgc
    28981 tacaaagaag acatcgaagc ctcgctgcgc acaccgggaa tcggcggcgt tcagctgctc
    29041 gacctgcacg acttccccgg ccagggcacc tcgctgatcg gcattctcga tccgttctgg
    29101 gacagcaaag gctacatcac cccggcggaa ttccggcggt tttacaatac caccgtgccg
    29161 ctgacccggc tgacccggca catctggacg accgaggaaa cgctgacaac cgacgttgaa
    29221 attgcgcatt tcggcgccgc tccgtttgcg gcggctcagc cttactggaa agtgctggcc
    29281 gacgacggac aagtggttgc cgccggcgat ctgccggttc aggccgtgcc gctcggaacc
    29341 cggacaaagc tggggcggat cagcctcgcg cttcgaaacc ttcccgcccc caatcaatac
    29401 aaactggtcg tcggcttgaa ggacactccg ttcgaaaacg attggaacat ttgggtctat
    29461 ccggccggga tgcccgccgc ccccgccgat gtgctgattg ccgagtcttt tgatgagtcg
    29521 gtttgccggc gtctggccga tggcggcaag gtgctgctgc tcgccgcgaa gcccggctcc
    29581 ggaaatccga agctgacgtt tctgccggtt ttctggaacc attacatatg gcagaagcgg
    29641 gaacagacac tcggtctgct gtgcgatccg aagcatccgg cgctggcgca gttcccgacc
    29701 cgctattttc aagactggca gtggtacgac atcctctcgt ccgccaacgg cattgtgctg
    29761 gaccgtctgc cgggcgactt gcgcccgatc gtccagccga tcgacgactg gaacaccaac
    29821 cgcaagctcg gcatgatttt cgagtgcaag gtcgggccgg gcaaactgct cgtctgtgcc
    29881 gccgatttgt cgaccgatct gagcgcccgg cccgccgccc ggcagcttcg cgccagcctg
    29941 ctggcctatg ctgcaagcgg agccttcaat ccgaaaactg aggttgaacc gtcggtgctg
    30001 actgaggctt tcagcctcgc cggcccgcca gctttggtta aaccccgcaa aggtaattga
    30061 gcgacagccg gtctttttcc cctgccgtca atctccttga cggaaatctg acacatgcac
    30121 tatgcaggcg ccgccctcac tgacaaggtg aggcggcgct caaccccgct gtccgcctgc
    30181 atttttggga aaaaagtttt ctgcgagccg gaaaaagcct attgttccgg gtctgaatgg
    30241 agattgagaa tgatcattgc cctggaattg gatccgttac gcatcctgag tatttaggaa
    30301 acttccgcga tgttgcatcc tgttatagcg tttattggat ccggcggcgg cgaactgctt
    30361 gtgattatgc tggctctatt gctccttttc gggccgaaag acgcgccgcg cattctccgc
    30421 agcatccaaa atgttctgaa taaactgcaa cgtgccgccg cagatttccg ttataagctg
    30481 atgtataccg atcttcactg gaatcagccg gaagccccta aagacagtac ggcgtgcgat
    30541 gctgaagaaa aaccggcaga agcgaagcct gccgaagaac cgccggaact cccgacggaa
    30601 catcctccgg ccgatgaacc caagacatga agaatctgtt taaaaaactg aggtcgcggt
    30661 ttatttatca cgacaaggag cagtcttttc tggagcatct ggaagagttt cgcaccatgc
    30721 tgatccgctg tctgctgacc atggcgatca ctacagtcat ttgcatcccg ctggcaaaac
    30781 ccctgcttca ctggctgcag gcgccattac tgcaggtggc ggctcagaat cactacactt
    30841 ttgcactgat taccacttcg cccatcgaag gattcatgca ggtgatgaaa gttatttttg
    30901 ccaccggcgt acttttgagt ctgcccttta ttatttattt tatcgcccag tttgtatttc
    30961 cggggctgaa acagaacgag cagaaaatgc tgatttacgg cggactggcc ggtgccgtcc
    31021 ttttcgctgc cggcgtggcg ctctgctaca ctgtgactct gccggtcgcc gttaatgtca
    31081 tgttttattt caatgactat ctgggaacga ccgcttactg gaaaatcgat acctatctcg
    31141 gctttgtcat gcaacttctg atcgggttcg gactggcttt tgagctgcct ttgattttgc
    31201 tgatgctggg ccggatgggc attatttcaa ccgcccagat gcgcaaatac cgccggcatg
    31261 tcgtcatcgt cattctgatt atcgccatgg tactgacgcc gccggacgtg ctctcgcaat
    31321 tacaaatggc tattccgctc tacatattat atgagctttg catcttgatt ttgcgcatcc
    31381 gcgaaagaaa tatgcgtaaa gaaaaggaag gaaccgaaga atgaatatac tcgcactcat
    31441 ccccggcatg gaggggccgt ccgaagtcct gctgattttt ctggcaattc tgctgctgtt
    31501 cggcggaaaa aaacttcctg agctggcccg atcactcggt aaaagtatcg gcgaattcaa
    31561 gcgcggacag aaggaaggtc atcaggacgc caaagaaagt gaaaaacctg aactgccggt
    31621 tccgcaggat cagcccgccg caaaatccgg agatgaaaaa aaaggagctg aacaatgaac
    31681 tgcctcgcca tattcggcat agagtcgccg gctgaaatcc tgctgattat ttttatcttt
    31741 gtactcctgt tcggcggaaa aaaacttcct gagctggccc gcgcacttgg aaaaagcata
    31801 aatgaattca aacgcggcca gacagaaagc gaacctgaaa agaagattga aaaacaggct
    31861 gaaccggccg atccggacga accagactcg accgcctcga aaaaataatt atttatagtc
    31921 gtccgggtta acgcccagct tctgtatccg gtagttgatg atacgttgcg tcgtgttgag
    31981 atagcgggct gtcgctgccg cattaccgcg atgcttttta agcgcatcaa tgataatttc
    32041 tttttcgtag ctctcaacca tcgttttgag gtctgctcca tcctttggaa agatggaagt
    32101 ctgcgtttcg tcgctggttt gcagcgacgg aggcagattg tacccgtgaa tgacttcgtc
    32161 ggaagcggtc agcacggcgc gctcaaggca gttttcaagc tcccgcacgt tgcccggcca
    32221 gtgataggcc atcatcatgt tgatggcgga ggtggagatg cgcttgatat tctttccgta
    32281 catttcgccg tatttctgga gaaagtggtc cgccagcagc atgatatcgc tgcggcgttc
    32341 acgcagcggc ggaaggataa tcggaaaaac gttaaggcgg tagtaaaggt cttcccgaaa
    32401 ggtttcttcg gcaatttctt tttcgagatt ccggctggtc gccgccagca ctctcacatt
    32461 gaccgaaaca ctttcactgc tgccgacccg ttcgaaggtt ctttcctgca atacgcgcaa
    32521 cagccgcacc tgtaccgacg gcgaaatatc cccgatttca tcgaggaaaa tcgtacctcc
    32581 attggccgtt tcaaaccgtc ccttgcgctg ctgagtggct ccggtaaagg cgcctttttc
    32641 gtggccgaaa agttcactct caatcaggtt ttcgggtaat gccgcgcagt tgacgctgac
    32701 aaatgcattg tttttccgct cactgccgta atgaatggcc cgggcaacca gctctttacc
    32761 ggttccgctt tcgccgcgaa tcagaacggt cgccgtgctg ttagccacct gaacaatctg
    32821 ttcgtaaacc gagcgcatgg cgctgcagtt gccgatgata ttatgcatcc ggtagcgatc
    32881 accgagctgg cggcgcagcc gttcattctc agtgagcagc ccttcacgct cttcaagctc
    32941 ttcgcgaata ctggccacgg cctcggcaag gatattggcg accagttcga ggaaacgctg
    33001 atagcggcgc agttcttcga gatcggtcgt aatcgagagg tcgatactca tggtcccgat
    33061 caccgctttc cggtaaatga caggcacaca gataaaagcg gtcggattgc ccgtgcgcga
    33121 tttcgtgaga ttcaggaagc ggggatcttt actgatatcg ggaatgagcg ccgactgtcc
    33181 ggtttgcgcc acacgcccgg tgaccccttc gcccagtttg tactgtcccc gttttttttc
    33241 ttctgcagtc agcccgcgcg aggcttcgat tacaaatatt tccgtgtcgg gtttgcgcag
    33301 agtgaatgtc ccgcgcgaaa cctccagctc agtctccatg atatcgagaa cttcgatcaa
    33361 cagttcagag acggtatgcg gatgagtcgc cgctgcctgc gatatctggt aaaggatcgc
    33421 cagttccttt tcgctctgct ctgcatagtc ggattttatt ggcatgatcg gttcccaatt
    33481 tgtattttgt tggtcatgta taaacaataa tgtaactgat ttccagcttt ttttaaattt
    33541 taaatctcat cgccacttca ggtggttcgc gaccccaact tgaagacgcc ctcttttgag
    33601 attcgttaat gtttttatat caatacttac agacaaaaat gtaacacact acctcttaga
    33661 gacacaagtg tacctttaag cgtgtataat agattgacgg accgtccaaa aagcgtacta
    33721 tccgcacgct tgagccagtg aggtttttct aatgaataaa aaagaagagt ttcaaaaagg
    33781 gtttccggaa ccgcaaggtc tataccatcc ggaaaatgaa cacgatgcat gcggagttgg
    33841 cttcatttgc aacctgcacg gaaaaaagtc gcatgacatt attcatcatg cactcgaaat
    33901 tctggtacgc ctcacccacc gcggagcttg cggctgcgac ccgctgactg gtgatggcgc
    33961 cggcattctt cttcagattc cccacgcttt ttataaaatt gagtgcgcca aactcggtat
    34021 agaactcccg gccgaacagg aatacggcac cggtctggtt tttctgccgc aggacgaagt
    34081 tcagcgcaag acctgcatgg aaatactcga acaggccgtt aaagaagaag gacagaaact
    34141 cctcggctgg cgcaaagtcc cgacctgcaa ccggctgatc ggcgaaaccg cccgcgcttc
    34201 cgaaccctac atcatgcaga tatttatcgg tcgcggcaaa ggcgtctccg acccgcgcac
    34261 cttcgaccgc atgctctata tcatccgcaa ggtcgccgaa aaccggattt tccattccgg
    34321 tctcaaagac aaaaacgact tttatatccc gacactcacc tgccggatta tcatctataa
    34381 gggactcctc ctccccgaac agatggaatt ctattatccc gacctgctcg acgacaattt
    34441 caccagcgcc ctcgctctcg tacaccagcg ctacagcacc aacaccttcc cggcgtggaa
    34501 actggcccag c
//
LOCUS       DAOPZO010000007        33634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_1213_length_33634_cov_18.430060, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000007 DAOPZO010000000
VERSION     DAOPZO010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 33634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33634
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1213_length_33634_cov_18.430060"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PKI68_00995"
     CDS             <1..354
                     /locus_tag="PKI68_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52299.1"
                     /translation="LLNADYSVIDNKPGGIGMVGDAPAVNMIDTVGMPTAVMFRWVGQ
                     GYDTFEIAQREMSIDEFGVVTYGAGYYPFIYLSTSKAEIFYPVTLHKYPTNRESATTG
                     QLYTIGTNELLYIKK"
     gene            370..747
                     /locus_tag="PKI68_01000"
     CDS             370..747
                     /locus_tag="PKI68_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52300.1"
                     /translation="MKKICIFSVLLFSALTGFSLDITPPSGAAINLPAARLEPVAITN
                     VVVSDLTVLLSGTGNRTENVYRVRCTFETARTNVQSVVTVKQTDADAMMAAEGYSLQD
                     ILAAARGAIGIMLNQKYGSVTNN"
     gene            766..1104
                     /locus_tag="PKI68_01005"
     CDS             766..1104
                     /locus_tag="PKI68_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52301.1"
                     /translation="MNFQRQDLAGAIRTKSFFEPVPISMGPNWKKIWIRACAVVLLLI
                     FAITLFAGCASQPKRHPFLRLDVQHTCREEIKFYFAQYRASEIDLQDLETLCLMCAEA
                     EAEKTAGETQ"
     gene            1101..1241
                     /locus_tag="PKI68_01010"
     CDS             1101..1241
                     /locus_tag="PKI68_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52302.1"
                     /translation="MKKISTTLAILAALQLAGCTAPQSVEFAPLIQLNDSANGNTVPI
                     RL"
     gene            1283..1549
                     /locus_tag="PKI68_01015"
     CDS             1283..1549
                     /locus_tag="PKI68_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52303.1"
                     /translation="MSDHECQKEEIIEEIRSRLGRGDVTLATLDLKLDQIKEQTTKTN
                     GRVTKLEDVTAGMKYTWKMVIRDVVVAFGTIGGLSATLIGIYGK"
     gene            1698..2258
                     /locus_tag="PKI68_01020"
     CDS             1698..2258
                     /locus_tag="PKI68_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52304.1"
                     /translation="MILLYRGKSFVSWRIKMATLSPYSHAAWLKTTPELRELIRTSPN
                     SAEAARAIIEAGCIEAWHIGPGVRETFDLHYGHSEGTVIDVFDLPELPDDRFSLVEAG
                     LRQELGKSYWFSGILNARFNCFLNRKPPMDSNGIILEWFCSMIIEHWLRLADFPTCSL
                     RRPAWGVWPGMFETSVHTKYLCSVKI"
     gene            2342..2605
                     /locus_tag="PKI68_01025"
     CDS             2342..2605
                     /locus_tag="PKI68_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52305.1"
                     /translation="MDPEEIFAEEFTKPVVLPNGIATSGIFDNQSATAFDVSTTAPQV
                     SLRQVDADLCRDGDPIWISGIEYRCKNKEYNDEGIVIVHLETV"
     gene            2711..3130
                     /locus_tag="PKI68_01030"
     CDS             2711..3130
                     /locus_tag="PKI68_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52306.1"
                     /translation="MRKQIRDTVKEVLEKLDGFPRGSVAIGRRHKFTAAQLPSVAIYT
                     DTEETERISMSPVKNMNTVDLVLRLDVKPDGVDLGEDKADALIAEMDSAILAGVKLIA
                     GIFDIVQTSLNFEGDGNSDSDYLQATRTYVVRYITVG"
     gene            3347..4021
                     /locus_tag="PKI68_01035"
     CDS             3347..4021
                     /locus_tag="PKI68_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52307.1"
                     /translation="MSKQVGSSGVITGAGIAGVNRFSITMAAESPEKTVMGAAVKAFM
                     AGITSFNFEAEGIGETPSLAETFTVSEGNLKSFDIEGVADTQDVTAIGDVGADDLPFR
                     SFIAGLAEWTANLNCDFEGAVPQRGAEYTFAGAAGIITSVSVNGEVNGLINATLQIKG
                     KGALPTGVNTAGTTINFSVACASGAIASGAAIVTSSKISGSVGGLITYSVNSQGTGEL
                     TIGEAA"
     gene            4021..4350
                     /locus_tag="PKI68_01040"
     CDS             4021..4350
                     /locus_tag="PKI68_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52308.1"
                     /translation="MAAIEPSEGLMCLEAFFKGLEKRSVEFSPIDKPVYARPLTCEQC
                     DMLAGKTGHAYNVRLIQAALVYEDGTPVFKETDIFRMMKSDPGVIGRTAMQISALIVT
                     SFDQIKN"
     gene            4503..6101
                     /locus_tag="PKI68_01045"
     CDS             4503..6101
                     /locus_tag="PKI68_01045"
                     /inference="COORDINATES: protein motif:HMM:NF021251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure C-terminal
                     domain-containing protein"
                     /protein_id="HNX52309.1"
                     /translation="MFGNDINVKIGATDSTGTAFRSAEMRMKSFAGTAKTMAGALGIA
                     FSAQAVVSFTKSVIDYGSAINDAATATNTNVEALQVFRYAAELGGAKAEQMDKALINL
                     NKSAVDASNGSKSAADAFRYLGINADTFINLPTDQKLTVLAQRMSQATDKNRAAAAAM
                     DLMGSKNAPKLMEVLKDLGENGYGSLESAAKRANAVMSKLDIKKLDDAGDGILKFQRG
                     MMVKSAPIVGFWGDVMLNGWNNAVDTVLDGNKKAVDSIKKSNVTISGVGSPDYSAFDS
                     FYKAGAAYSKYRDKLIAEDRSKDAMRKEMGMSPIDRSWIEWVRVFGDTWSAQMDASAT
                     RTAKDLGIIRGDMTATQVHWASVAKGIEGYTNTMASNMTDAIMEFAETGKMSFKSFAN
                     SVIQDMIRIMITESITRPIAGAFTGAISGGIGAMFGGGGGAAAGGGAAAGGASASGGP
                     VYAGRSYLVGEKGPEIMTTKSDGRVIPNEALSSGGGSTVINFNVTAMDSASFSTHLSK
                     HKAEISGIVESAYKRRGKRGPMTS"
     gene            6485..6691
                     /locus_tag="PKI68_01050"
     CDS             6485..6691
                     /locus_tag="PKI68_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52310.1"
                     /translation="MVTADSNSDAAGKASISINTPLCASPEDSAAVYSDCKFRCSLVD
                     DNTDTEVSTDMTYDFKVQLMEVLY"
     gene            6691..7251
                     /locus_tag="PKI68_01055"
     CDS             6691..7251
                     /locus_tag="PKI68_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52311.1"
                     /translation="MARDITSAVKTAAEQTRISTCHLLTVYLDAQTIYWCDYTRPVIF
                     DGNTYSPAGQLLGFEGLEETSDVVVNSVTVSLSAVDTNATSLILSNYYLDRRLTVHRA
                     FFDVNDALIPDPIIIFDGRMNKPSLVENPEDGSAVLSVEASNQFADFERKPGRHTSND
                     EQQIHFPGDKGFEYATEIDQTLTWGK"
     gene            7211..7675
                     /locus_tag="PKI68_01060"
     CDS             7211..7675
                     /locus_tag="PKI68_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52312.1"
                     /translation="MRLKSTKPSLGENSHPRFPDWESRLHDFANEVMEKPFVLGETNC
                     SMLAARAVDVMYETDFFNEFRLVAPSDESELSEALSRRTLREFEAHGFSKVEKNFEQT
                     GDVLIGWKEPFERCAVYLGGGRCLTSSREHGVRLVPLNVFVRAYEPEVFRWV"
     gene            7666..10971
                     /locus_tag="PKI68_01065"
     CDS             7666..10971
                     /locus_tag="PKI68_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNX52313.1"
                     /translation="MGVETVIILGITYGMVYQAIATALISFAISYAAQKLFGVTPETP
                     KFDQSITENIDGATNPIPIIYGSRRVGGTRVFVETSGASNTYLHTVLAMCEGEIHSID
                     DILLSDISITDAKFSGSAWGYKHTGADDQTVDASLNGLLPDKWTENHRLQGTAYVYVR
                     LKFNQNIFSSIPTILCDIKGKKVYDPRTETSAWSDNPALCLRDYLTNTRYGRGIPVEM
                     IDDDAIIAAANYCEEQVTFKDPSGSAYNDNRYRCCGAINPDDGYLDNVKKILSSCRGM
                     LIFSSGKYKLVIDKPETAAFTFNEDNIVGKINIGMGDKSIVFNRMRVRYFDTDLGYEQ
                     NYFTYDNPTLRANQDNGLILEGDLELPLTNEIVRVEQIAQMEVKRSRQSIVCQFDSTL
                     EALKNDVGDVVNVTYLRAGWPNKKFRVVKIGLKSEDTVEVSLKEYDASVYVMEAWVPP
                     GEPDTLTPGLWNYDPPNNVSAASGNTELLIAGDGSLLSRLRLSWTAPENGYCTGFEVG
                     YKLSSEENYTDFAKGKEEAQHIFSPVQDGVDYDLRVRAVYFNGGHSEWVYTLDHTVQG
                     KSALPDTPDGFSVTNEPNNVKKFVWNSVGNLDLRGYRLRMKSGSTGEWSEMTSLHSGN
                     LAGSPWETGSISETGAHRFGLVSVDTSGNESLPVYFNMTLESTGESSTPPGAPSGLAA
                     TAMYLSVFLEWTNPTENFSATEVWISATNDRAAAVRLAVTSAPIFQIEREDETPFFVW
                     LRSLNSAGTPGPFNSDDDDGLECEALIVIDSTSAAAITATAAKIANLYAANIAAGSIS
                     SGTVIIGPGGAIRSHDYEHGNNGWFFGHDLAEVCNLIIRNAQLIGGIQQSLNFQTGAQ
                     GAYINWETGDAEFHTLRVYGSVAAPVLFIGGQAITGGYYVDAVDVSVVTMEGTTVRYT
                     TDGTVPDETDAAVSGGTLTISASNNYVFVAYEDSTGRASIPVICPFRIARPLSTGGFT
                     PFEFYIRIRSYFDTDYVLIQRNNNDPTTGAWIALPKKKHYRSSGILDGFVFEDTYDFE
                     TGSNLSNQNLIYCQSSTNAITGRIGPIYKRVYNVNDASMPWGTSIYVWTGSAWGSAIT
                     APGGTLDPDDYPG"
     gene            complement(10968..11555)
                     /locus_tag="PKI68_01070"
     CDS             complement(10968..11555)
                     /locus_tag="PKI68_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNX52314.1"
                     /translation="MLNNPFSMVQRADQSGSGHGRAFTRGEEAALLAVCREIGTEWHW
                     YEASVLARYTGLRLIDIASLKREDIHGDRIELVPSKTERHEIKVTIPLHPKAKAALDH
                     LPARGFLFEKLAHHYATTKKRTGYTDLIRSAKIEPKGAVLSFHCWRHTFRTRLAEAGV
                     AQDVAMKLGGWTNAATAEIYNHDLTRAEAAIMALD"
     gene            complement(12241..12756)
                     /locus_tag="PKI68_01075"
     CDS             complement(12241..12756)
                     /locus_tag="PKI68_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52315.1"
                     /translation="MNGDRSRKTRTWGKYMKLTAIFILISAFFIFSGCKTITEQQRAE
                     INGYLDYTPDQIPRMDDKSLVIIIRDWKKQCPNTMSNMIHLADEELLKRYPSFYQDYK
                     NGKIAVGMPEHLALMAWPHHLDEPDNVWATASGTRRQFCYRYQNAGIPVTYKWFVTQN
                     GTVTMFEESPY"
     gene            complement(12753..13220)
                     /locus_tag="PKI68_01080"
     CDS             complement(12753..13220)
                     /locus_tag="PKI68_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX52316.1"
                     /translation="MSKITNIGAATLTALNETGMRQEELARHMKSTQASVSRLIAGKN
                     RPSLQTLHALCSALKSRNYHQAVLVLIGHLHDEIEASGMLLSDIDMSPARSKAPGRAD
                     IEKYIDTIRAGAVKSRETASLVKDLAWLIGHANLESDTLMMVAEKPAEYGKKK"
     gene            13541..13912
                     /locus_tag="PKI68_01085"
     CDS             13541..13912
                     /locus_tag="PKI68_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52317.1"
                     /translation="MNGVEKMAKDLAAAKETMDAMLIKIGEQAKDIAFLLERSRGAWS
                     FVLGGDRDCGASSNSIVYIAYGLIPIEEQVFPSDQADLDACRRMLDKLPIHRITKDVH
                     EAMFRATQALAVKQREFAGRS"
     gene            13909..15204
                     /locus_tag="PKI68_01090"
     CDS             13909..15204
                     /locus_tag="PKI68_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HNX52318.1"
                     /translation="MSQRTFKMVDLFAGAGGFSEGFRRTCERLGIPFDLTAINHWPTA
                     VETHSVNHPSARHFCESVYEVDPRDVAPDGYMDCLTASPECIYHSNARGGGVCDDQSR
                     SGAKDILRWVSAIYVQTIVIENVKEFMGWGPIYKSGPNKGLPIPSRKGAEFRLFCVGL
                     RELGYHIDFSVLNSANYGAYTARERFFMIACKGIPFSGWPELTHAKNPDRPFVKQWHA
                     ARDIIDWKLQGDSLFMRQSGMLEGKAPLVKNTIDRIVWGLKNISGIDPEPFLVMLYGT
                     GKARSIDRPLPAVTTSGNHIALAEPFFAKYHGGESGKTRCYGVDAPLMTLDTSNRFSP
                     IEPFLVKYFGSGANAVSTNGPLPTVTTKDRFGLIEPCLLDVRYRLLEPHELAAGMGFN
                     GYKFCGTKTDIKKQIGNAVEVNQSTAIAERVVMNWRAAA"
     gene            15201..15641
                     /locus_tag="PKI68_01095"
     CDS             15201..15641
                     /locus_tag="PKI68_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52319.1"
                     /translation="MIAFLNLQDILFSVSAPETRGVRRLHKISHPSAIRRGVANDGSP
                     FGRLCIGAVCRCAEKTARLWRIADLPKDGEIRYLPRSRKAGHGSRKPLLEATDRRDRV
                     RFFYDENGELCRFATRRECALIIAGCLLASACLVALIFLSGGVF"
     gene            15665..15895
                     /locus_tag="PKI68_01100"
     CDS             15665..15895
                     /locus_tag="PKI68_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52320.1"
                     /translation="MSNEVKTFELKTVVCPLFASYPAVAELFPGLGRNKLNALVDAGL
                     IRRKKTGDLPTDGVLYRVADILAYMESSDRAA"
     gene            15935..16168
                     /locus_tag="PKI68_01105"
     CDS             15935..16168
                     /locus_tag="PKI68_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52321.1"
                     /translation="MYENEIRKVKKLVGVQKMSMGLAITQVADSSGIPVFKLAQEMRK
                     HAHASQASKKYAARYKATVPNRMIDSSQLEVRG"
     gene            16186..16500
                     /locus_tag="PKI68_01110"
     CDS             16186..16500
                     /locus_tag="PKI68_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52322.1"
                     /translation="MAEKMMFSAERDEADAIKSGLQLALDAVDKAGADAVETTVIADL
                     KIAHQKICLAITFLNSGESDLGAGVRPIDARAKRVPKLMAGAPEPYKRAGFGRKCRKL
                     CE"
     gene            16640..16906
                     /locus_tag="PKI68_01115"
     CDS             16640..16906
                     /locus_tag="PKI68_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52323.1"
                     /translation="MAAKNTKIGGVMAGGELKLEVSIEAAVHDAFKGIAERMVKDHGF
                     IIRNVNISWTTMLTANGRGALIERISIDSETIHGTEIILMNPKA"
     gene            16910..18196
                     /locus_tag="PKI68_01120"
     CDS             16910..18196
                     /locus_tag="PKI68_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52324.1"
                     /translation="MASTFTDEQLDRAFKAVTVIKVAANFGIALKLGVQKSPFRKDTH
                     GASFSVFEGGFKFKDHADDDTHGGSWKMAQLCNPALSRKEIRNLLVSLNGEIPKALTK
                     GQVKREVAVKRKELYQAAADNAEEIPRLDIQEPGHWSVPIRERWEAGIPAVIDQAEKL
                     ASARGWDADLIVELADAGKTSSPLLPWADAGDRRGWGWLVEKPVFKAGRLSLVSVGYH
                     ERYYIYSKPETGNLKPEREKRWVYVPSIPKDQESGGRHQESEFQKHLRALGCRIPAYP
                     FILGDPEHEPRLCVILEGQFDAVSFAAACGWFGDTSTGIPPGVFVFGLRGVQSQKALL
                     AGYGLWLRKYNPFVWIIGDNDPAGRKIDAMKDVKAIAAEPSFIDRLRAQGCRVHAELI
                     HHSGCKDFNDVYKAARPSIEIMKKWAGHVGAGDFVK"
     gene            18193..19617
                     /locus_tag="PKI68_01125"
     CDS             18193..19617
                     /locus_tag="PKI68_01125"
                     /inference="COORDINATES: protein motif:HMM:NF040238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HNX52325.1"
                     /translation="MSEFDPTKNENLDEGREASAAFVGRTMMQTLGLTDRVFFDESQG
                     LYWMQDAGGEWRGRKEKLFADYLKRCGVNAVRDDKSKMSDLDLLLLHISLNCAVAFAG
                     PLAGYKKGLIRNNGMSILVTRSFEIPVPVKGAIDIVLAVIDQQLGPCGEDGIDQRDYL
                     FGWWQHSLNCLIHGYKKNMGMCLTLAGTKGCGKSLLKKLIKLSLGGRECQPYNYLSGK
                     DNFNGEFLRCECWCVDDDQSQTDAKMRDEVASKIKKIVADETFHVRGKHVEALDIHLY
                     RRLIMCVNDEPEKLLVLPQLTDDIVDKILILHCRKPQDEANPMPMPVRTPQEQDAFGA
                     ALESQLPAFMWWLLNEYKIPDDMYGRFGIRHFQHPDIKRILFDLSPEVALMDAIERVL
                     FGPDASFWTGSASDLRVELIAESSPLSSREKNNVRSVQWIGRQLARIADQHPERYRQR
                     SGRSDGRVWEICKSGRKLSELDGR"
     gene            19824..20417
                     /locus_tag="PKI68_01130"
     CDS             19824..20417
                     /locus_tag="PKI68_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52326.1"
                     /translation="MQISMKSDIAKATAKLRDAQRKQIPFAVSNALSDTAWDVAKEEA
                     HQMTIKLDRPIPFSVKAFGVIRATKAKLVATVFIKPIQAAYLKFQVEGGTRRPKGKAM
                     PVPGDIRLNQYGNMTKGKIKALLADPRNFSGVIHGNGGIWQRQKNGGVKLLVAWEQTA
                     KYEANRFPFYKIAVGKAKAVIGSNLRKAIASALRSAK"
     gene            20656..21291
                     /locus_tag="PKI68_01135"
     CDS             20656..21291
                     /locus_tag="PKI68_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52327.1"
                     /translation="MSLNMAAFGKELGVSRATVSLWKKDGMPTCDRDAAISWLAGKHP
                     HYVEKLAGEIVGAGAGNAPRGAGGSVEGLDLPSTLARFREIEFRAWQDLDSALLKRKE
                     ADAGSAKARDLDAAISRLSKRYKDSAEDRLGMEKRVADFELETGRRVGMDRVNEFIND
                     KFAGLVVLIKALPYTESRNVNPNDPETAKRELGRWVNNLLRQQREEMAEND"
     gene            21284..23245
                     /locus_tag="PKI68_01140"
     CDS             21284..23245
                     /locus_tag="PKI68_01140"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HNX52328.1"
                     /translation="MINLHEQRCWKSIRRHYRDIFAEDPDQTVWEWCEQNVVLTELET
                     DYPGLLDTDLTPWIREPLEAVRDDEVEEITVIAGTQVIKTLFIICAIAWWAKHRGTRM
                     LHVMDTKDNASDFAESRLQPIFQNSESLRQLIPSDRNKWNKLRMYIGRALLNLTGSNS
                     PGNLASRPAPFVSMDEVGKFREKTEKETDAVSLAKSRTKSKTRRKIIITSSPTYEFGL
                     EWKAFKSGSQEVWKIQCPHCGEYIELRKDGIRWCPLAKRGGKWDMDFVADTAHYVCQL
                     CGGQFGSSEKRKLNRSGRWSVQNPRAPRNVRSFRIPSYYSPWESCSFGNVAVHFLRCK
                     AEFNMKDFDNNYDAMPSFDEVEKLEWEILKGRKEKYAQPYPAMAAYCTGFFDVQKGWF
                     EWFCVAWGENNEHWVVEHEIVNADPSDLDEWKSTLDVLDRQRPIPLEWAFYDFGGNWH
                     ANAIRFVRSLGRQNVQLSFGSIDDKLPEQGRRTWTKKDVKPRTRLFEIGASQAKMKIY
                     KMLSRDVPGRGYCHFPEFLDDEYFKQLCAEERRPKTEKGRTVYYWHKSRERNESIDGH
                     VGCYCGKLQIPVAKRKRLYLEAAERRKEVRASELVPDQSDPSDASETIKPKAQGLKPS
                     SPLPPPPSPAKPVVQRVQRRAPRGWMQGY"
     gene            23264..23524
                     /locus_tag="PKI68_01145"
     CDS             23264..23524
                     /locus_tag="PKI68_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52329.1"
                     /translation="MKPIKFKEVNVTYAENQPEYLPLPVYRKPGAECEVVSCWKLTRR
                     ERLKILITGKFWLSIWTFGHPLQPILPTVNKTDLIKAEGGAE"
     gene            23521..23697
                     /locus_tag="PKI68_01150"
     CDS             23521..23697
                     /locus_tag="PKI68_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52330.1"
                     /translation="MNEAMMKELEDPDTLLIARLPMDVKRFAALAGLFERGEAVVKAM
                     PAGCQWTVIKKARG"
     gene            23703..23909
                     /locus_tag="PKI68_01155"
     CDS             23703..23909
                     /locus_tag="PKI68_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52331.1"
                     /translation="MTYTPGGKGIAPRQSERPSWIGLEEIGRDALTVTFKTKTGTRTV
                     PLADVEKYERRIGSKPHSNKGKLK"
     gene            23972..24349
                     /locus_tag="PKI68_01160"
     CDS             23972..24349
                     /locus_tag="PKI68_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HNX52332.1"
                     /translation="MFFDHIISGKKIHTIRANAKGYYKDGDVVQITQWSGAPYRSKVE
                     KTGVVIRIGLEPVTLALLDNGTLSATVGFKTVLPVRLAQNDGLPLERFIDWFFPLRKP
                     GIFKGSIVHFSSFRYSKGTNEKA"
     gene            24336..25244
                     /locus_tag="PKI68_01165"
     CDS             24336..25244
                     /locus_tag="PKI68_01165"
                     /inference="COORDINATES: protein motif:HMM:NF019146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HNX52333.1"
                     /translation="MKKHKIGWLNVPGYIPQTWNPIVGCSKVSEGCRNCYAERMANRL
                     ARMMNFEYMTATGGGGGWNGETILCEPTLKKAFGWKKPHAIFGNSMGDLFHENTPDEW
                     IDRVFAVMALCPQHLFIVLTKRAERMEKYFSEQWRSAYVEGMAQKIYNERTGEDPSMW
                     LAVHFPLPNVWLGVTAENQAAADERIPHLLRTPAAVRFVSCEPLIGPVNVSLYLPFTT
                     RGRGGAHRETGGLDWIICGGESGPGARPMNPEWARSLRDQAQAAGVPFFFKQWGRFAG
                     GYRELPVTHSDVLDGQQLHQWPEVKK"
     gene            25241..25630
                     /locus_tag="PKI68_01170"
     CDS             25241..25630
                     /locus_tag="PKI68_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52334.1"
                     /translation="MNKPELVIITGLPGTGKSTLAKNEFPNHLHYEPDHLFCDSRGAY
                     RFDAQLWSLAIDWVVNMTDFALARGESVVVSDVFENIEDLERFKILSIYHGVELRMIE
                     CKERFGSIHHVPRFVMNRMDERLKGLK"
     gene            25627..25974
                     /locus_tag="PKI68_01175"
     CDS             25627..25974
                     /locus_tag="PKI68_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52335.1"
                     /translation="MSKLLRCPWCHKTSDDKDNDTRMVAVELVTFPRGDESAEYSVCC
                     NWCGATGAIADTTDGALEGWVAEDHLQILEEKALRNLRDVVRAEIRERDRKTGFFDEA
                     KAWTNIRNALSAC"
     gene            26049..26387
                     /locus_tag="PKI68_01180"
     CDS             26049..26387
                     /locus_tag="PKI68_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52336.1"
                     /translation="MKNPVDPVILSKKSRSAYQRKYHAEHLEEAAERKELERYRREKA
                     RRYWRTKILLKREMPGLIADVSPEILEQERIKYMALLDEPCEVIHVPAEVVRKRKVTT
                     EEEEEAEEEN"
     gene            26454..26672
                     /locus_tag="PKI68_01185"
     CDS             26454..26672
                     /locus_tag="PKI68_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52337.1"
                     /translation="MTFLLPNGNELMSCKELAAALKRTPQYVSAMRRDGFIMPGDRAT
                     VNHALIWLHEHPQFRQNKRHCRLPVVDC"
     gene            26830..27210
                     /locus_tag="PKI68_01190"
     CDS             26830..27210
                     /locus_tag="PKI68_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52338.1"
                     /translation="MGKRPLPSIFNRQSAIVNEFDEPIREVVSGFIPMGMTRVETAET
                     LGVSVASLRTFCEKTDIRFPRNQPGRAERIRDTMRRGPRARRLSLNGRSQCLSAWAEE
                     YGIHYETLRKRLDRGRTLREALRV"
     gene            27314..27841
                     /locus_tag="PKI68_01195"
     CDS             27314..27841
                     /locus_tag="PKI68_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52339.1"
                     /translation="MNPASIYGGLSAERFYNLPKFPAGAYTLRARIVGAETPVEITAA
                     QCTGSGADWRVLIPAASLAALATGDYTLLIIATDSAGAEQLAAKETIKFYAAAETDLR
                     SDLHKMLDALNATLAKKATRDQASMSYNGRSISRLSWDELLKARDAIARQVRAEENSL
                     AGRSRIQTVKMRFIE"
     gene            27955..29376
                     /locus_tag="PKI68_01200"
     CDS             27955..29376
                     /locus_tag="PKI68_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HNX52340.1"
                     /translation="MSRNYAFGRPSRTADDFFSGSLSADQAVFGSLYAMRNRARQLER
                     DDSHVRKFLIMAKTSIIGAKGIRLQSKAGDFIKGQFYPSRFDQDLIETAFEDFSKSWN
                     FSIDGKLDRRRFAQVAIQRVLVDGECIIRRHRGAGMYGIQNQLIDAELLDHTLNQPAS
                     SNRNEIRMGVEIDANGKPLAYHFLEEAPPAWMGTAVSTANRKRIPANDINHIFIQERP
                     GQTRGVTWLAPTGLRARMLDGIETAVTVGYRVAASKMGFFRPGENYEGEEIETADIPS
                     DVTPGELNLLPKGIEFQSFDAGYPNAEFDGFKKSIVREIAGGLGVSYPELGNDFGGVS
                     YSAGQIGVHSDAALWSDLQQFWMDSFEEPNFREFLLMSITSGELQLPLSKLRKFQNVR
                     FQPQRRKHIDPLKTHNAQRIALGDMSRSPFDIAAENGADFEDVIDETKAAIEMLKAAG
                     LPVPESWASGKALSTIIEEPPAT"
     gene            29406..31361
                     /locus_tag="PKI68_01205"
     CDS             29406..31361
                     /locus_tag="PKI68_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HNX52341.1"
                     /translation="MNELKKILARIAEEGGQLNRAAIIKPVLVNPTRAEGEDAQYRFE
                     LSFSSETEKVKRWFGTEILGHQPGEIRLERLQSGNHPLLLMHDHDRQIGVIESVKLEN
                     SRGTAVVRFGKSALAQEIRQDVIDGIRSNVSVGYHIHDMKLIKESDEEGDTYRVTDWA
                     PYEISIVPVPADVDVGIGRSAELNHPKINQEKKMTELEKRALAMGLRKDASEESVREA
                     ELAHARKEGERAAKEKGDAELARQNGIRELAVAHRERLIDADTMAEKAIKDGTTLDAF
                     RSEILVAYKGKSPALKSSRADGDQRKLLEGFSLRKFMLGAVTGESLKGAEAEVQSEGQ
                     REASARGMDVGPGYIPAMAIGEIAKRAELIRDGQNATTNADGKYLVATENMGLIGALS
                     PRLWMKQLGVTMLSGLVNNITLPTIEAEGEAVDRTEIESDSSVDVTLGQRTLSPTRIP
                     ASCTFSSQLLRQTSPQIDSVLSNILLSRIARRWNAKGIEFLLALSGTGSVVTSGDVLT
                     HAIMRGFLTKLGMENADEAPGAFLINPDVEGVLAATKVDAGSGLFLHTEKENGEGRIL
                     NRRSLTTSLVPNDLGDANDLSAIIFGAFPRLWMGDWGGVDLIRDNVTKAATGQLVLTA
                     ASFIGFAAESPKYFVKAVDVDPAAVAG"
     gene            31458..31814
                     /locus_tag="PKI68_01210"
     CDS             31458..31814
                     /locus_tag="PKI68_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52342.1"
                     /translation="MKNVIAIIAVALIAVSAVKAQNYQTIDMAAYGTTYTATNAQSFG
                     QTLYTSAAITYDSANITNSITFYAKIDGINYLLGTSSVTNGQYDFIDLNPLALRPGSA
                     LVINTTQTNVNIRLGK"
     gene            31901..32092
                     /locus_tag="PKI68_01215"
     CDS             31901..32092
                     /locus_tag="PKI68_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52343.1"
                     /translation="MKIKITNEVVMSGGWFLPGAVIEIDDAKAAILISEGKALAHSEV
                     LKIAVKSGAFAWIKKLVKK"
     gene            32089..>33634
                     /locus_tag="PKI68_01220"
     CDS             32089..>33634
                     /locus_tag="PKI68_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52344.1"
                     /translation="MKKTSARSVLSAICILLFALSSPAAVRPISTAWDIQNGNKRLVD
                     INFFQAESVRLTLQTTDGANAYRLTNSNLIVVWEITGWNDYTNVYSIATGTVISATNG
                     ISSYSLTPAQANLSPGTYRGYVRALQTVSNQLDQVAVLAWQRIKVEYSPDSRNYNIVG
                     PLTYPPIYTEADLEAITNQIGSLQSEVVSLSNRVDAAESAITNLQSQMSTVTGWGDHA
                     GLYPLMAAWNATNSALWNAMGNRLETSVFTGWTNAQHSINTNLAAIVTLATNDISGLK
                     SGTGVWNSVTGKVDRTDATYTGTVATAAAAFGWGDHALAGYLTSAPPQTVTSVNGLTG
                     AVVITAASIGAATGTLLSVSNGTATNLTVVDTLEVKAQAVSQSGLLSGSQLPAEFAGE
                     YTYCGTEQMYMSGMQLFTNSFGNFMFIENGYFCGQYGSWTIGPSQGGVPYGSSTNCYS
                     LIDGVWYQFDANEGPIATTDIQTVRLPFYSASNGNVRAENLVSGKGLLLNADYSVIDN
                     KPGGIGM"
BASE COUNT         8106 a         8792 c         9325 g         7411 t
ORIGIN      
        1 ctgttaaatg cggattattc ggtaattgac aacaaacccg gcggaatcgg gatggttggt
       61 gacgctccgg cggtaaatat gattgacacc gtagggatgc cgaccgcagt catgtttagg
      121 tgggttgggc aggggtatga tacgttcgaa attgcccagc gcgaaatgtc aattgatgag
      181 ttcggcgttg taacttacgg ggcaggatat tatccgttta tttatttaag cacctctaaa
      241 gcggaaattt tctatcccgt tacactccac aagtatccca caaacaggga gtccgccact
      301 acaggacagc tttatacaat cgggacaaat gagttgctgt acattaaaaa ataaccaagt
      361 gaggatatta tgaagaagat atgcattttt tcagttctgc ttttttcggc gctgaccggc
      421 ttttccttag acatcacacc gccgtcaggc gccgccatca atcttcctgc ggcccggttg
      481 gagccggtgg cgattacaaa tgtggttgtg tctgatctta cggtacttct ttccggcact
      541 ggtaatcgca ccgaaaacgt gtaccgcgtc cgttgcacgt ttgagacagc aagaaccaac
      601 gtacaaagcg ttgttacggt aaagcagaca gatgcggatg caatgatggc ggcggaaggc
      661 tacagccttc aggatatttt agcggccgca cggggtgcta tcgggataat gcttaatcag
      721 aagtatggtt ccgtaacgaa taattaagag cgttcgatgg atgttatgaa cttccaacgc
      781 caagaccttg ccggggcgat ccggacgaag agcttctttg agccggtgcc aatcagtatg
      841 gggccgaact ggaaaaagat atggattcgc gcctgcgcgg tcgtcctgct tttgatcttt
      901 gccatcacac ttttcgccgg ttgcgcatct caaccaaaaa gacatccatt tttgaggctg
      961 gacgttcagc acacctgccg agaagagatt aaattttatt tcgcccaata ccgcgccagc
     1021 gagatcgacc tgcaagacct ggagacgctc tgcctgatgt gcgccgaggc cgaagcggaa
     1081 aaaacagccg gagaaaccca atgaaaaaaa tcagcacaac cctcgccatc ctcgccgccc
     1141 tccagctcgc cggctgcacc gctccgcagt ccgtcgagtt tgcccctttg atccaattga
     1201 atgactcggc aaacgggaac accgtgccaa tcagacttta ggacgtaggc tttaggaatt
     1261 ttaatacagg aagaaagtaa ccatgtcaga tcacgaatgc caaaaagaag aaatcattga
     1321 agaaatccgc tcccgccttg gacgcggaga tgtaacgctg gcaactctgg atttaaagct
     1381 cgatcagatt aaggagcaga cgaccaagac caatggacgt gtaacgaagc tggaagatgt
     1441 caccgctgga atgaagtaca cctggaaaat ggtcatccgc gatgtcgtcg tggccttcgg
     1501 aacaatcgga ggtctgtcgg caacgctgat tgggatttac ggaaaatgaa cgcctgccga
     1561 acaatctgcc ttaccgtcgt catctgctgg gcgctgctga tgctaaacat ctggtatgcg
     1621 ctgaagaatc accagtcaac gacggtagag cggacaacgc gggtatgggt taaagcgcag
     1681 gcttgcggag gtgaacaatg attcttcttt atcgcgggaa atcgtttgtg tcgtggcgga
     1741 tcaagatggc tacgctttcg ccttacagcc acgccgcttg gctcaagaca acgccggagc
     1801 tgcgggagct tatccgtacc tccccgaact ccgccgaggc cgcaagggcc attattgagg
     1861 ctggctgtat tgaggcgtgg cacatcggcc ccggcgtgcg cgaaacgttc gatctgcatt
     1921 atggccatag cgaaggcacg gtgattgacg ttttcgacct gccggagctg ccggatgatc
     1981 ggttttcgct ggtcgaggct ggcctgcggc aggaacttgg gaaatcctac tggttcagcg
     2041 ggatcctgaa cgcccggttt aattgctttt tgaaccgcaa gccgccgatg gattcgaacg
     2101 gaatcatact ggagtggttt tgctcgatga ttattgagca ttggctgcgc ctggctgact
     2161 ttccgacctg ctccctgcgc cgtcctgcgt ggggtgtctg gccgggtatg ttcgaaacga
     2221 gcgtccacac gaaatatctg tgcagtgtga aaatatgaca gccagttgca agcaaccggc
     2281 tgtacagaga agtaaagacg gttgctcgca accggctatc aggaattgag cggagaaaac
     2341 tatggaccct gaagaaattt ttgctgaaga gtttaccaag ccggtcgttt tgccgaacgg
     2401 catcgccacc agcggaattt tcgataatca gtcggcgacg gccttcgacg tgagcacgac
     2461 agcgccccag gtgtcgctac gccaggtaga tgctgatctg tgccgagatg gagatccgat
     2521 ctggatttcc gggatcgagt accgctgcaa aaacaaagaa tataacgacg aaggcatagt
     2581 cattgttcac cttgaaaccg tttaagagag gctttaggcg ttaggctata ggctttagaa
     2641 accttatgaa acagaagaaa aacccaaagt ctaaggccca aagtctaagg ccttccggag
     2701 cacagcaact atgagaaaac aaatccgcga cacagtaaaa gaggtgcttg aaaagctgga
     2761 cggatttccc cgtggatcag tcgcaatagg tcgccgccac aagtttaccg ccgcccagct
     2821 tccctccgtc gcaatctata ccgacaccga agaaactgaa cggatttcaa tgagtccggt
     2881 taagaacatg aatacggttg atctggtcct ccgactggac gttaagccgg acggcgtgga
     2941 tctcggcgag gataaggccg acgccctgat tgctgaaatg gattcggcaa ttctggccgg
     3001 cgtaaaactg atcgctggaa tctttgatat tgtccagaca tctctgaatt ttgaaggcga
     3061 tggaaattca gattccgact acctgcaagc gacccgtacc tatgtggtgc ggtatataac
     3121 cgtaggctaa acctgaaatt tgaaacctga aacttgaaat agcttcatca tccccgatca
     3181 gtttctggtc ggcccccgct gtgatacgtc gcggcgggtt ttttattcct ttcacgtttc
     3241 cggtttcagg gttcaggttt cctccagcct ccgcctgatc taccggacag ccgcttaacc
     3301 tatctcctat tgaagatcgt ttagatcaat tcaacgggag agaattatgt caaaacaagt
     3361 tggaagttcc ggagttatta ccggcgcagg tattgccggt gtaaaccgtt tttcaatcac
     3421 gatggcggca gagtcgcccg aaaaaaccgt tatgggtgcc gcggtcaaag cgttcatggc
     3481 cggaattaca agctttaatt ttgaggcgga gggaatcgga gaaacacctt ccctggctga
     3541 aacctttact gtgtcggaag ggaatttgaa atcgttcgat atcgaaggag tggcagatac
     3601 gcaggatgta accgccattg gagatgttgg ggctgatgac cttccattcc gttcatttat
     3661 cgccgggctt gccgaatgga cagcgaatct taactgtgat tttgagggtg ctgttccgca
     3721 gcgcggagcg gaatatactt tcgccggagc tgccggaata atcaccagcg taagcgtcaa
     3781 cggtgaagtc aacggcctga tcaatgcgac tctgcaaatc aaagggaaag gcgctcttcc
     3841 gactggcgta aacaccgcag ggacaacgat caacttctcg gtggcctgcg ccagcggagc
     3901 catcgcatcg ggtgcggcca tcgtaaccag ctccaagatc agtggatcgg tcggcgggct
     3961 gataacctat tccgtcaact cgcaaggcac cggcgaactg actatcgggg aggccgcgta
     4021 atggctgcga tcgaaccaag tgaaggcctg atgtgtctgg aggcgttttt taaggggctc
     4081 gaaaagcggt ctgtagaatt ttctccgatt gataagcctg tttatgcgcg tcctctgaca
     4141 tgcgagcagt gcgacatgct ggccggaaaa accggacatg cctacaacgt acggctgatt
     4201 caggccgcgc tggtttatga agatggaacg cccgttttta aagaaacaga cattttccgc
     4261 atgatgaaat ctgaccccgg agtaatcggc agaacggcaa tgcagatatc tgctttgatc
     4321 gtcaccagtt ttgaccaaat aaaaaactga tgcagtcgcc gggcggcggg cggctgagag
     4381 aaaaatacgc agtggcagag gctctcggga aaacactcgg agaaataaaa gagatgcctg
     4441 aaaacgagtt catgggctgg gtcgcttttc tttcgatgcg cgggaggctt taaggatttc
     4501 aaatgttcgg aaacgacatc aatgtaaaaa taggcgcgac ggattccaca ggaaccgcct
     4561 tccgttcagc agaaatgcgc atgaagtcgt ttgccggaac cgcaaaaaca atggccggcg
     4621 cgcttggaat agcattcagc gcgcaggcgg tcgtttcgtt tacaaaatcc gttatcgatt
     4681 acggctctgc cattaacgat gcggcgactg ctacaaatac aaatgtcgaa gcgttgcagg
     4741 ttttccgata cgccgccgag cttggtggtg ctaaagccga acagatggat aaggcgttga
     4801 ttaatctcaa caagtcggca gtggatgctt ccaatggctc aaaatcagcg gccgacgcct
     4861 tccgatatct cggaattaat gcagatacat tcatcaattt gccgaccgac cagaagctca
     4921 cggtgcttgc tcagcggatg agccaggcga ccgataaaaa ccgcgcagcc gccgcagcga
     4981 tggatttgat gggctcaaaa aatgctccga agctgatgga ggttttaaaa gacctgggcg
     5041 agaacggata cggctcactc gaatcagcgg caaagcgcgc caatgccgta atgagtaagc
     5101 tggatattaa aaagctcgat gatgcgggtg atggaatcct taaatttcag cggggaatga
     5161 tggttaagtc ggctccgatt gtaggatttt ggggcgacgt gatgcttaat ggatggaata
     5221 atgctgtcga cacggttctt gatgggaata aaaaggcggt tgattcgatt aagaaatcaa
     5281 acgtaacgat cagtggagtc ggatctcctg actattcggc gttcgacagt ttttataagg
     5341 ccggcgcagc ctattcgaaa tatcgcgata agctgattgc tgaggatcgc agcaaagatg
     5401 ccatgcggaa agaaatggga atgtccccga tagatcgctc ctggattgag tgggttcgtg
     5461 tattcggtga cacatggtcc gcacagatgg atgcttccgc aactcgtacg gctaaagatc
     5521 ttggcatcat tcgcggtgat atgacggcca cccaggtaca ttgggcgtcg gtcgctaaag
     5581 gaattgaagg ctatacaaac acgatggcct ccaacatgac cgatgccatt atggagttcg
     5641 ccgaaaccgg caagatgagt ttcaagtcgt ttgccaattc agtgatccag gacatgattc
     5701 gaatcatgat cacggaaagc attacaaggc cgatcgccgg agcatttact ggggcaattt
     5761 ctggcggaat cggcgcgatg ttcggcggag gcggcggagc agcggctggc ggcggcgctg
     5821 cagccggtgg cgccagcgcg tccggcggac cggtctatgc tggtcgctct tatctggtcg
     5881 gcgaaaaagg cccggagatc atgactacga agtcggatgg ccgcgtgatc ccgaacgaag
     5941 ctctcagttc gggcggcgga tcgaccgtga tcaatttcaa cgtgacggcg atggattccg
     6001 ccagcttttc tacccatcta tccaagcata aggcagagat cagcggaatt gtcgagtcgg
     6061 cctataagcg ccgcggtaaa cgagggccaa tgacatcatg agcactttcc catccagccc
     6121 ggctccgcgg tctgcaaaaa taaaaagcgt gtcgccgacg tttgtatcga ccgcgcaaag
     6181 cctgcggcgg gttgtcagtt cacgcggagc ccaccgctgg gcaattgaat tggatttccc
     6241 tccgatgcgg cgttcgatct ttgcgccact atgggcgttc ataaccgctc agcgcggaag
     6301 atccggatcg tttgactttg ttcttcctgc tcatgctccg caaggaagct gggctggaac
     6361 cgttctggtt gatggagccg cgcaagtcgg tactacgatc aaccttaaag ggtttacagc
     6421 ggctcaaaat ggagtcgcaa aggccggtga ttttgtacgc ttcgccggat cttataaaac
     6481 atacatggtt acggccgact cgaacagcga cgccgccgga aaagcttcta tttcaatcaa
     6541 tacgccgctc tgcgccagtc cggaagattc cgccgcagtt tattcagact gcaagttccg
     6601 ctgctcgctg gtggacgata ataccgatac cgaggtttct accgatatga cctatgattt
     6661 caaggttcag ctgatggagg tgctgtacta atggctcgag atattacatc tgcggttaaa
     6721 acggcggcag agcagacgcg catttcgaca tgccatctgc tgaccgttta tctagacgcg
     6781 cagacgatct actggtgcga ctatacccgt ccggttattt ttgacgggaa tacatattca
     6841 cctgccggtc agcttttagg gtttgagggg ctcgaagaaa caagcgacgt ggtagttaat
     6901 tcagtcaccg tttcgctttc tgcggttgat acaaacgcga ccagcctgat cctgagcaac
     6961 tattatctcg accggcgttt gacggtccac cgcgcgtttt ttgatgtcaa tgatgcgctg
     7021 attcctgacc cgatcattat ttttgacgga cgtatgaata agccgtcact ggttgaaaat
     7081 cctgaagacg gatctgctgt actttcagtc gaggcttcaa accagttcgc cgactttgag
     7141 cgtaagccgg gacgtcatac ttcaaatgat gaacagcaga tacactttcc aggcgataaa
     7201 ggatttgaat atgcgactga aatcgaccaa accctcactt ggggaaaata gccatccgcg
     7261 ttttccggac tgggaatccc gtctgcatga ctttgccaat gaagtcatgg aaaagccgtt
     7321 tgtgctgggc gaaaccaact gttccatgct ggccgcccgc gctgttgacg tgatgtatga
     7381 aacagatttt tttaacgaat tcaggctggt agcgccgtcc gatgagtctg agctgtcaga
     7441 ggctttatca cgccgcacac tgcgcgaatt cgaagctcac ggattttcaa aagttgagaa
     7501 gaactttgag cagaccggag atgtactgat cggctggaaa gagccgtttg aacgctgtgc
     7561 ggtttatctg ggcggaggtc gctgtctgac cagctcgcgt gagcatggtg ttcgccttgt
     7621 tccgctcaat gtttttgtcc gcgcctacga accggaggtt ttcagatggg tgtagaaacc
     7681 gtcatcatcc ttggaatcac atacggaatg gtatatcagg ccatcgcaac ggctctgatc
     7741 tcgtttgcca tttcttatgc cgcccagaag ctgttcggag ttactccgga aactccaaaa
     7801 ttcgatcaat ccattactga aaatattgat ggtgcaacca atccgatccc gatcatctac
     7861 gggagccggc gcgtcggcgg aacccgcgtt tttgttgaaa cgagcggagc atcaaataca
     7921 tatcttcata ctgttctcgc tatgtgcgag ggcgaaattc attcgataga cgacatcctt
     7981 ttgagcgata tttcaatcac ggatgctaag ttctccggtt cagcctgggg atataagcat
     8041 accggggctg atgaccagac ggtcgatgcc tcgctgaacg gtcttttgcc ggacaaatgg
     8101 actgaaaacc accgacttca gggtacggct tatgtgtacg ttcgccttaa atttaaccag
     8161 aatattttca gctcgatccc tactatcctt tgcgacatca aggggaaaaa ggtttatgac
     8221 ccgcgtaccg aaacatccgc ctggagcgac aatccggcgc tctgtctgcg tgattatctg
     8281 acaaatacac gttacggtcg cggcataccc gtagagatga ttgacgatga cgcgatcatt
     8341 gccgccgcaa actactgcga agagcaggtg acattcaaag acccgtccgg ctcggcgtat
     8401 aatgataacc gctaccggtg ctgcggtgcg attaatccgg atgacggcta tcttgacaac
     8461 gtgaaaaaga ttctgtcgtc atgccgcgga atgctgattt tcagttccgg aaaatacaag
     8521 ctggtgatcg ataagccgga aaccgccgcc ttcacgttca atgaagacaa tatcgtcggc
     8581 aaaatcaaca tcggtatggg cgacaagtcc attgttttca accggatgcg cgtccggtat
     8641 tttgatacag atcttggcta tgagcagaat tatttcacat acgacaaccc gacccttcgc
     8701 gcgaatcagg ataacgggct tattctggaa ggtgatttgg agctgccgct tacgaatgaa
     8761 attgttcgcg ttgagcagat cgcccagatg gaagttaagc gatcgcgcca gtcaatcgtc
     8821 tgtcagtttg attcaaccct cgaagctctg aaaaacgatg taggtgatgt cgtgaacgtt
     8881 acctacttgc gcgccggctg gccgaataaa aagttccgcg tggtaaaaat cggccttaaa
     8941 tctgaggata ccgtagaagt cagcctgaaa gaatacgatg catctgttta tgttatggaa
     9001 gcatgggttc cgccgggcga gccggatacg ctcacgcccg gcttgtggaa ctatgaccct
     9061 ccaaacaatg tgtctgccgc cagcggtaat acagaacttc tgattgccgg tgacggctcg
     9121 cttctttcgc gcctccgcct ttcgtggaca gcccctgaaa acggatactg caccggattt
     9181 gaagtcggct ataagctgtc cagcgaggaa aattatacgg actttgcgaa aggaaaagaa
     9241 gaggcccagc atattttttc gccggtgcag gatggcgttg attacgatct gcgcgtccgg
     9301 gcggtctatt tcaacggtgg tcattctgaa tgggtttata cgctggacca taccgtgcag
     9361 ggtaaatcgg ctctgccgga tactccggac ggattctccg tcacaaatga gccaaacaac
     9421 gtcaaaaaat tcgtctggaa ctcggtcggc aatctcgatc tgcgaggcta tcggttgcgc
     9481 atgaaatccg gctcgaccgg cgagtggtcg gaaatgacct ctctgcactc cggaaatctc
     9541 gccggatctc cgtgggaaac aggatcaata tcggagactg gagcgcaccg gtttggcctt
     9601 gtttcggtcg acacatccgg caatgagtcg cttcctgtgt atttcaatat gactttggaa
     9661 agcaccggag aaagctctac gccgccagga gcgccgtccg gactggccgc aacggcgatg
     9721 tatctgtcgg tatttctcga atggacaaac ccgaccgaaa acttttcggc aacggaggtc
     9781 tggatttcag caaccaatga tcgagccgcc gcggttcggc tggctgttac ctccgcgccg
     9841 atctttcaga ttgagcggga agatgaaacg ccgttctttg tctggcttcg ctcgcttaac
     9901 tccgccggaa cgccaggccc gttcaattcg gacgatgacg acggcctcga atgcgaagcg
     9961 ctgatcgtca tcgactcaac cagtgccgct gccatcaccg ccaccgccgc caaaatcgcc
    10021 aatctgtacg ccgccaacat cgccgccgga tcgatcagct ccggaaccgt catcatcggc
    10081 ccaggcggag ccatacgcag ccatgattat gaacatggaa ataacggatg gttttttggc
    10141 cacgacttgg ccgaggtctg caacctgatt atccgcaatg cgcaattgat tggagggatt
    10201 cagcaaagcc ttaatttcca aaccggcgcg caaggggcat acatcaactg ggagaccggc
    10261 gacgctgaat tccatacgct gcgggtttat ggctctgtcg ctgctccagt tttatttatc
    10321 ggcggtcaag caattaccgg aggatactac gtcgatgcgg tcgatgtcag cgttgtaaca
    10381 atggaaggaa cgaccgttcg ctacacaacc gacggaacag ttcctgatga aaccgacgct
    10441 gctgtttcag gcggtacgct gaccatttcc gcatcaaata attatgtgtt cgtggcttat
    10501 gaagattcga ccggacgcgc cagcattccg gttatctgcc cattccgaat tgctcgtccg
    10561 ttgtcaaccg gtggcttcac gccgtttgaa ttctatatcc gaatcagatc ttattttgat
    10621 accgattatg tcctcatcca gagaaacaat aacgacccga cgactggggc atggattgcg
    10681 ttgccgaaga aaaagcacta cagatcgagc ggaattttgg acggatttgt ttttgaggac
    10741 acctatgatt ttgagaccgg atcaaacttg tcaaaccaaa acctgatcta ttgccagtcg
    10801 tcgacgaacg ccatcactgg gagaatcggc ccgatttata aacgggttta taatgtcaat
    10861 gatgccagca tgccatgggg aacgtcaatt tatgtatgga ctggatcggc gtggggatcg
    10921 gcaatcaccg cgccaggcgg cacgctggat ccagacgact atccgggcta atccagcgcc
    10981 atgatcgccg cctctgcgcg ggtcaggtcg tggttataaa tttctgcggt agccgcattg
    11041 gtccagccgc ccagcttcat cgccacgtcc tgcgcaacgc ccgcctcggc cagccttgtg
    11101 cgaaatgtat gacgccagca atgaaacgaa agaactgccc ctttcggttc gatttttgcg
    11161 gaacggatca gatcggtata tccggtgcgc tttttcgtcg tcgcgtaatg gtgagcaagc
    11221 ttttcgaaca ggaaccctct cgctggtaga tgatccagcg cggcctttgc tttcgggtgc
    11281 agcggaatag tcacctttat ttcgtggcgc tcggtttttg atggaaccag ctcgatccgg
    11341 tccccgtgga tatcctcgcg tttaagcgat gcgatatcga tcagccgaag ccccgtataa
    11401 cgagccagca cgctcgcctc gtaccaatgc cattccgttc cgatctctcg gcatacggcc
    11461 agcagagcgg cttcctcgcc gcgtgtaaac gcccggccat gaccactccc gctttgatct
    11521 gccctttgca ccatagaaaa cggattatta agcattcctg cgcgcgccgc gacggccgca
    11581 aaaacagttt tcaagtttcc gacccggtta ttttgagact ttcctccggc cggctgatcc
    11641 gcttccagcc agtccgcgaa cgcctgagcc aggcgcggag ttacatcatg aagaaatttc
    11701 acttcaggcg aatggcttgt aatccactta atgaactgcg ccgaaattct gcggcggcga
    11761 tcgagcgtat cctttccggc ctgcggcttt gtttgcagat aaattttcca aagatctgca
    11821 accggagatc ctgaacttcc tccggcttcc cagccgatca gcgaatccac cgccgataca
    11881 agcctcgacc gcgccatatt cccgcgcatc gcggatctga ttgaagcgtc aaccgcctcg
    11941 gctgtcgcct gatctgatgt cccggacgaa aaccgttttc gttttccatc ctgcccataa
    12001 atcgaaatcc accagacggc gcaatcgggt cgtttgtaaa tgctcatgta ttataaataa
    12061 tataaataaa ccacaacagc aagacacaaa aaagacacta taaaaacaga aaaccctgta
    12121 tttattgggt ttaaatggtg gaggtggggg gaatcgaacg tctatatttt tcgtaaactg
    12181 tttatgtgtg cattataaaa catataaaca aagtttgttt ggtcacggag ctgacacggt
    12241 ttaatatgga gattcttcaa acatggttac tgtgccattt tgcgtgacga accatttata
    12301 agtaactggg attcctgcat tttggtatcg ataacaaaac tgccggcgcg ttccagatgc
    12361 tgtggcccat acgttatcag gctcatccag atgatgaggc cacgccatga gtgctaaatg
    12421 ttccggcatc ccaaccgcta ttttaccgtt tttatagtcc tgataaaagg aagggtatcg
    12481 cttcaacagc tcttcatctg ccagatgaat catattcgac atggtattcg gacattgctt
    12541 cttccagtcg cgaataataa tgaccaggct tttgtcatcc attctgggta tctgatcggg
    12601 tgtataatcc aaatatccat ttatttcagc acgctgctgt tccgtaatag ttttacagcc
    12661 agaaaaaata aaaaatgcgg aaattagtat gaatatcgcc gttaacttca tgtattttcc
    12721 ccaggttcgg gttttacgac ttctgtcgcc gttcattttt tcttcccgta ttcagccggt
    12781 ttttcggcga ccatcatgag ggtgtcgctt tcaagattgg cgtggccgat cagccatgcc
    12841 aggtctttta ccagcgatgc cgtttcgcgg gatttaacgg ctccggcgcg gatggtgtca
    12901 atgtatttct cgatgtcggc gcgcccgggg gctttgctgc gcgcaggtga catgtcgatg
    12961 tcggacagaa gcattccgct ggcctctatt tcatcgtgca aatgcccgat cagtacgagc
    13021 accgcctggt ggtagtttct tgatttcagt gccgagcata gcgcgtgaag cgtttgtaat
    13081 gatggtctgt ttttaccggc gatcaaccgg cttactgatg cctgtgttga tttcatgtgt
    13141 ctggcaagct cttcctgccg cattccagtt tcgttcagtg ccgttaaagt tgccgcccct
    13201 atgtttgtta ttttgctcat cccctactcc tttcgtgctg gtttatacgc gaaacgtata
    13261 aaataaaatt tatgttgtca aacatcttga cttactttgt ttgctattat acgttgcgtg
    13321 caagatacga aacggacagc aactatgata acgaaaacgc gaatagaaaa aagtgtcgga
    13381 cacatgctga ggatcggatc gaagaacgcc gatccggttg aggcggctat gtcgaagcat
    13441 ttgacggcag tacttgaaaa ggcggctagt gatttcagac ggacgatgcg gaaacgcagt
    13501 cctgttgcgg cgtaaatcta acctgaaagg cgggttggtg atgaatggtg tggaaaagat
    13561 ggcaaaggac ttggcggcgg ccaaggaaac gatggatgcg atgctgatta agatcggcga
    13621 gcaggcgaag gatatcgcgt tcctgctgga acgctcgcgg ggagcttgga gttttgtgct
    13681 gggtggcgat cgggactgtg gagcgtccag caattcgatt gtttacatcg cgtacggtct
    13741 gatccctatc gaagaacagg ttttcccgtc cgatcaggcc gatctggacg cctgccgccg
    13801 gatgcttgat aagctgccga tccaccggat cactaaagat gttcatgagg cgatgttccg
    13861 cgccactcaa gcgctggctg ttaaacaacg tgaatttgct gggcggtcat gagtcagcgc
    13921 acgttcaaga tggtcgatct gttcgccggt gccggcggct tctcggaagg tttccgccgg
    13981 acgtgcgaac ggcttggaat cccgtttgat ctgacggcaa ttaaccactg gccgacggcg
    14041 gtcgagaccc attcggtgaa tcatccgtca gcccgccatt tctgcgagtc ggtctacgaa
    14101 gtagatccgc gcgacgtagc gccggatgga tacatggact gcctgaccgc cagcccggag
    14161 tgtatctacc actccaacgc ccgcggcggc ggcgtgtgcg acgatcagag ccgcagcggt
    14221 gccaaggata ttctgcgctg ggtgtcggcg atatacgttc agacgattgt tatcgagaac
    14281 gtgaaggaat tcatgggctg ggggccaatc tataaaagcg gcccgaataa gggactgccg
    14341 attccgtcac gcaaaggcgc tgagttccgg ctgttctgcg tcggattgcg cgagcttggc
    14401 taccacattg atttttccgt gctgaacagc gcgaactatg gcgcatacac ggcccgtgag
    14461 cggttcttca tgattgcctg caaggggatt ccattctccg gatggccgga acttacgcac
    14521 gccaagaatc cggatcggcc gttcgtgaag cagtggcacg ctgcgaggga tattatcgac
    14581 tggaagttgc aaggagacag cctgttcatg cgtcaatccg gaatgctgga aggaaaagct
    14641 ccgctggtta aaaacaccat cgaccggatt gtctggggat taaagaatat cagcggcatt
    14701 gatccggaac cgttcctggt catgctgtac ggaaccggta aggcgcggag cattgaccgc
    14761 cctcttccgg cagttaccac cagcggaaat catatcgccc tggccgaacc attctttgcg
    14821 aagtatcacg gaggcgagag cggaaaaact cgctgctacg gcgtggacgc tccgctgatg
    14881 acgctggata catcgaaccg cttcagcccg atcgaacctt tcctcgttaa atatttcgga
    14941 tccggagcga acgcggtttc gacaaacggc ccgctgccga ctgttaccac gaaagaccgg
    15001 ttcgggctga ttgagccctg cctgctcgat gtccggtatc gcctgctgga accgcacgaa
    15061 ctcgcagccg gcatgggatt caacggctac aaattctgcg ggacaaaaac cgacatcaag
    15121 aagcagatag gcaatgcggt ggaagtgaat cagtccacgg cgatcgccga gcgcgtggtg
    15181 atgaactgga gggctgcggc atgatcgctt ttttgaatct tcaagacatt ttgttttccg
    15241 tctccgcacc ggaaacccgt ggagttcgcc ggctccacaa aatcagtcac ccgtccgcga
    15301 tacgtcgcgg cgtggcaaac gacggctcgc ctttcggccg tctatgcatc ggagccgtat
    15361 gccgatgtgc tgaaaagacg gcccgccttt ggcggattgc tgatttaccg aaggacggag
    15421 aaatccgcta tctaccgaga agccggaagg caggccacgg ctcgcgcaag ccgttactgg
    15481 aagcgacaga ccgccgcgac cgggtgcgct ttttttatga tgagaacggc gagctgtgcc
    15541 ggttcgcaac gcgacgggaa tgcgcgttga tcatcgcggg ttgtcttttg gcgtcggcct
    15601 gtctggttgc tctgattttt ttgtccggag gtgtgttttg acggcatccg ctgggagatt
    15661 ccgaatgagt aatgaagtaa aaacgttcga gttgaagacg gtggtctgtc cgctgtttgc
    15721 cagctatccg gctgtcgctg agctgttccc tgggcttggc cgtaacaagc tgaatgcact
    15781 ggttgatgca gggttgattc gtcgaaaaaa aaccggagac cttccgaccg atggagttct
    15841 ttatcgtgtg gcagatattc tggcgtacat ggagagcagc gatcgggcgg cgtagacgaa
    15901 gcctgagtga aaatagaaac ctgaaagggt caatatgtac gaaaatgaaa tcaggaaagt
    15961 taagaagctg gttggtgttc agaagatgag tatgggtctg gcgattacgc aggtggctga
    16021 ttcgtcgggg attccggttt tcaagctggc gcaggaaatg cgcaaacacg ctcacgccag
    16081 tcaggcctct aaaaagtacg cagcccgtta taaggcgacc gttccgaacc gtatgattga
    16141 cagttctcag ttggaagttc gcggttaaaa aaaaaggagg cgggtgtggc ggagaagatg
    16201 atgttttcag cagagcggga tgaagcagat gcgattaaga gcggattgca gttggcgctt
    16261 gatgccgttg ataaggccgg agctgatgcc gttgaaacaa cggttattgc tgatcttaaa
    16321 attgctcatc agaaaatctg cctggcgatt acgttcctta attccggaga gtccgatctt
    16381 ggcgctggtg tgcggccgat tgatgcgcgg gcaaaacgcg ttccaaaact aatggccggt
    16441 gctccggaac cttacaagcg ggccggtttc ggccggaagt gccgcaagct gtgtgaatga
    16501 tttatgggga gaatatcagg ggttatcggc ggctttgttg tggttgctga acgactaacc
    16561 tcccgatgcc agacggcaaa ggctccccgc catttttaca ggatggtttc cagtgcgcag
    16621 atgctgacaa ggtgagattt tggccgcaaa aaacacgaaa attggagggg ttatggcggg
    16681 tggtgaattg aaattggaag ttagtattga ggcggcggta catgatgcgt ttaagggtat
    16741 tgctgagagg atggttaaag accatggctt tattatccgg aatgtaaata tttcgtggac
    16801 tactatgctt actgcgaacg gccgcggcgc gctgattgaa cgaatttcga ttgattcaga
    16861 aacaattcac ggcacagaga ttattttaat gaatccgaaa gcctgatcta tggcatctac
    16921 cttcacagat gagcagcttg accgtgcatt caaggcggtg acggtgatta aggttgccgc
    16981 gaatttcggc atcgcgctga agcttggcgt tcagaagtcg ccgttccgta aggacacgca
    17041 cggtgcgagt ttttcggtgt tcgaaggcgg gtttaagttt aaggatcacg cggatgatga
    17101 tacgcacggc gggtcgtgga agatggctca gctttgcaat ccggcgctgt ccagaaagga
    17161 aatccgcaat ctgctggtat cgctgaatgg cgagatcccg aaggcgctta ctaaaggcca
    17221 ggtgaagcgc gaagtggcgg ttaagcgcaa ggagctttat caagcggctg ctgacaatgc
    17281 cgaagagatt ccgcgtctgg atattcagga gcccggccat tggagcgttc cgattcgtga
    17341 gcgctgggaa gccgggattc ctgcggtgat tgatcaggct gaaaagctgg cctccgcgcg
    17401 cggctgggac gccgatctga ttgttgagct ggcggacgcc ggcaagacat cgtccccgct
    17461 gctcccgtgg gctgatgcgg gcgatcggcg cggctggggc tggctggttg aaaagccggt
    17521 tttcaaggcg ggcaggctta gtctggtttc tgttgggtat catgagcggt attacatcta
    17581 ctcgaaacct gaaaccggaa acctgaagcc tgaaagagaa aagcgctggg tttatgtgcc
    17641 gagcattccg aaagatcagg agtccggcgg aaggcatcag gaatcagaat ttcagaagca
    17701 tctgcgggcg ctgggttgcc gcattccggc ctatcctttc attctgggcg atccggagca
    17761 tgagccgcgg ctttgcgtga ttctggaagg ccagttcgat gcggtgagct ttgcggcggc
    17821 gtgcggatgg ttcggcgata cgtcaacggg tattccgccg ggtgtgttcg ttttcggtct
    17881 gcgcggcgtt cagtcgcaga aggcgctgct ggcgggttac gggctttggc ttcgcaagta
    17941 taatccgttt gtctggatca ttggcgataa cgatccagcg ggccgcaaga tcgatgcgat
    18001 gaaggatgtg aaggccattg ctgctgagcc gtcgtttatc gaccggttgc gcgcccaggg
    18061 atgccgtgta cacgccgagc tgatccatca ttcgggctgt aaagatttca acgatgtcta
    18121 taaggcggcg cggccgtcaa tcgaaatcat gaaaaaatgg gccgggcatg tcggcgcggg
    18181 ggatttcgtt aaatgagtga atttgatcca actaaaaacg agaaccttga cgaagggcgt
    18241 gaggcgtcgg cggcgtttgt cgggcgtacg atgatgcaga cgcttggtct gacggatcgt
    18301 gtattcttcg atgagtcgca ggggctttac tggatgcagg atgctggcgg cgagtggcgc
    18361 gggcggaagg agaagttgtt tgccgattac ctgaagcgct gcggcgtgaa cgctgtccgc
    18421 gacgataaga gcaagatgtc cgatcttgac ctgctgctgc tgcatatctc tctgaactgt
    18481 gcggtggcgt ttgccgggcc gctggccggc tataagaagg gtttgatccg gaacaacggg
    18541 atgtcgattc ttgttacgcg gtcgttcgaa atccccgtcc ctgttaaggg cgcgatcgat
    18601 atcgtccttg ccgtcattga tcagcagttg gggccgtgcg gcgaagacgg gatcgatcag
    18661 cgggattatc ttttcggatg gtggcagcat tcgctgaact gtctgattca cggctacaag
    18721 aaaaatatgg ggatgtgcct gacgctggcc ggcacgaagg gctgcggaaa gtcgctgctt
    18781 aaaaagctga tcaagctgtc cctaggcggg cgtgagtgcc agccgtacaa ttacctgtcc
    18841 ggtaaggata atttcaacgg ggagtttctc cgatgcgagt gctggtgtgt ggatgacgat
    18901 cagagccaga ccgacgcgaa gatgcgcgat gaggtcgcca gcaagatcaa gaagattgtt
    18961 gccgatgaga cattccatgt gcgcggaaag catgttgagg cgctggatat ccacctttac
    19021 aggcgtctga tcatgtgcgt aaacgatgag cccgaaaaac tactggtgct tcctcagctg
    19081 accgacgaca ttgttgataa aatcctcatc ctgcactgcc gcaagccgca ggatgaagcg
    19141 aatcccatgc cgatgccggt ccgtacacct caggagcaag atgcgttcgg tgccgccctg
    19201 gagtcacaac tgccggcgtt catgtggtgg ctgctgaatg agtacaagat tcctgatgat
    19261 atgtatggcc gcttcggaat caggcatttc cagcatcctg acatcaagcg catcctgttc
    19321 gatctgtctc ctgaggttgc cctgatggat gccattgagc gcgtcctgtt cggccctgat
    19381 gcatcgttct ggactggtag cgcttccgac ctgcgcgtcg agcttatcgc ggaatcctcg
    19441 cccctatctt cgagggagaa aaataatgtc cgttccgtcc aatggatcgg gcgtcagctg
    19501 gcgcgtatag ccgatcagca ccccgaacgc taccgccaaa ggtcgggacg ttctgatggt
    19561 cgtgtttggg aaatatgcaa gtcaggccgg aaattaagcg agttggatgg acggtaaatg
    19621 acatcaaagg acacatcttt caaaaatcga acagccggaa accgcgttga tttccaacga
    19681 tttataaaaa cgcaggacat taggacatca ttttctaaat tatatatgtg cgcacaggtg
    19741 cgtttattaa ggatgcgcgc atgtgtgtgc gggatatttt ctgaaaatac cgtcctgatg
    19801 tcctgtctcc ggaggatcgc ctaatgcaaa tctcaatgaa gtccgacatt gccaaggcaa
    19861 ccgccaagct gcgcgacgcc cagcgtaaac agatcccatt tgccgtatcc aatgccctga
    19921 gtgataccgc ctgggatgtg gccaaagaag aggcacacca gatgacaatc aaactcgacc
    19981 gtccgattcc gttctcggtt aaagcgttcg gtgtgatccg ggccactaag gcgaagctgg
    20041 tcgccactgt attcatcaaa ccgatacagg ccgcctacct gaagtttcag gtggaaggcg
    20101 gaacgcgccg gccgaaagga aaggcgatgc cagtacctgg tgacattcgg ttgaaccagt
    20161 acggcaacat gaccaaggga aagatcaaag cgctgctcgc cgatccacgc aacttcagcg
    20221 gcgtgatcca tggcaacggc gggatatggc agcgtcagaa gaacggtggc gtcaagctgc
    20281 tggtcgcatg ggagcagacg gccaagtacg aagccaaccg cttcccgttc tacaagatcg
    20341 ccgtcggaaa agccaaggcc gtcatcggta gcaatctacg caaggcgatc gcatcggcac
    20401 tgaggagcgc aaagtgattt caaacgtcca aaggtactgt gggataattt tatcgagggt
    20461 aattcgcgac ctcgtttgtt ttatgcctgt caagtctgtc cggttgcttg accgtttttg
    20521 ttcagaatcg cgttcagaag gcgtcaagac ggctcttaga gcgttgagcg gagcttttga
    20581 aaagggacgg tggggacgtt ttgaaagatg ggttctgtgc tcaagtaggg gcggattttt
    20641 ggaaggcggt gcgctgtgag tttgaatatg gcggcgtttg gtaaagagct gggggtgtcg
    20701 cgggctaccg tgagcctttg gaaaaaggac ggaatgccaa cgtgtgaccg ggatgctgcg
    20761 atttcctggc tggctgggaa gcatccgcat tacgtcgaaa aactggccgg tgagattgtc
    20821 ggcgccggtg ccggtaatgc tccacgtgga gcgggcggta gtgtcgaagg tcttgacctt
    20881 ccgtctacgc tggcgcgttt ccgtgaaatt gaattccgtg catggcagga tttggacagt
    20941 gccttgctta aacgcaaaga ggccgacgcc ggatcggcga aggctcgcga ccttgacgcg
    21001 gcgatttcgc gcctgtcgaa acgctacaaa gattcagctg aggatcggct cggtatggaa
    21061 aagcgggtcg ctgactttga actggaaacc ggacggcgcg tcgggatgga ccgcgtgaat
    21121 gagttcatca atgacaagtt cgccgggctg gttgtactga tcaaggctct gccatacacc
    21181 gaatcacgca acgtaaaccc gaatgatccg gaaacagcga agcgcgaact tggccgctgg
    21241 gtcaataacc tgctacgcca gcaacgtgag gagatggctg aaaatgatta acctccacga
    21301 acagcgctgc tggaaatcga tccgccggca ctaccgcgac atcttcgcgg aagatccgga
    21361 ccaaacggtt tgggaatggt gtgagcagaa cgtagtcctg accgaactgg aaacggatta
    21421 tcccggcctg ctggataccg accttactcc gtggatacgc gaaccgctcg aagccgtccg
    21481 cgatgatgag gtcgaagaaa taacggtcat cgccggaaca caggtcatca aaaccctgtt
    21541 catcatctgc gcaatcgcct ggtgggcaaa acaccgcggc acccggatgc tgcatgtcat
    21601 ggacaccaaa gacaacgcat cggactttgc cgaaagccgc ctccagccga tattccagaa
    21661 ctcggaatcc ctgcgccagc tcatccctag cgaccggaac aaatggaaca agctcaggat
    21721 gtacatcggc cgcgccctgc ttaacctcac cggctccaat agtccgggaa acctcgcctc
    21781 gcgccctgcc ccgttcgtat cgatggatga ggtcggaaag ttccgcgaaa aaacggaaaa
    21841 ggaaaccgat gccgtatcgc tggcgaagag ccgcaccaaa tcaaagacac gccggaaaat
    21901 catcatcacc agctcgccga cctacgaatt cggccttgaa tggaaggcgt tcaaaagcgg
    21961 gtcgcaggaa gtctggaaga tacagtgtcc gcactgcgga gagtatatcg aactccgcaa
    22021 agatggaatc cgctggtgtc cgctcgccaa gcgcggcgga aagtgggaca tggacttcgt
    22081 agccgacacc gcgcactacg tctgccagct ttgcggtgga cagttcggct ccagcgaaaa
    22141 gcgcaagctc aaccgatccg gccgctggtc cgtacaaaac ccccgggcgc cgcgcaacgt
    22201 ccggtcattc cgcataccgt cctactactc gccgtgggaa tcctgctcat tcggaaacgt
    22261 cgccgtccac tttcttcgct gtaaagccga attcaacatg aaggacttcg acaataacta
    22321 cgacgccatg ccgtcctttg atgaagtcga aaaactcgaa tgggaaatcc tcaaaggccg
    22381 caaagaaaaa tatgcgcaac cgtatccggc catggccgcc tactgcaccg gcttcttcga
    22441 cgtacagaaa ggctggtttg aatggttctg cgtcgcctgg ggagagaata acgagcattg
    22501 ggtcgtcgaa catgaaatcg tcaacgccga tccgtccgac ctcgacgaat ggaaatccac
    22561 gcttgacgta ctcgaccggc agcgcccgat cccgctcgaa tgggccttct atgacttcgg
    22621 cggaaactgg cacgccaacg ccatccgctt tgtccgcagc cttggccggc agaacgtaca
    22681 gctctcgttt ggatcgatcg acgacaaact ccccgaacag ggacgccgca cctggacgaa
    22741 gaaagacgtc aagccgcgca cccgcctctt tgaaatcggg gccagccagg ccaaaatgaa
    22801 aatttataaa atgctctcgc gcgacgtacc gggccgcggc tactgccatt ttccggaatt
    22861 tctggatgat gaatacttca aacagctctg cgccgaagaa cgccgcccga aaacagaaaa
    22921 aggccgcacc gtctactact ggcacaaatc gcgcgaacgt aacgaatcca tcgacggcca
    22981 tgtcggctgc tactgcggga aattgcagat ccccgtcgcc aagcgcaaac gcctatacct
    23041 ggaagccgcc gaacggcgga aggaagtccg ggcttcggag ctggtgccgg atcagtccga
    23101 tccgtcggat gcgtccgaaa caataaaacc taaagcccaa ggtctaaagc cttcctcccc
    23161 gcttccaccc ccgccttccc ccgccaagcc ggttgttcaa cgagtacagc gccgcgcccc
    23221 gcgcgggtgg atgcagggat attaaacgaa cgaaaggaat aaaatgaaac caataaaatt
    23281 caaagaagtg aacgttacgt atgcggaaaa ccagccggaa tatcttccgc tgccggtcta
    23341 tcgtaagccg ggtgccgagt gtgaggttgt gtcgtgctgg aagttgacgc ggcgcgaacg
    23401 ccttaaaatt ctgatcactg gaaaattctg gctgtccatc tggacgtttg gacatcctct
    23461 tcaaccgatt cttccaaccg tcaataaaac cgatctgatc aaggcggaag gcggtgcgga
    23521 atgaacgaag caatgatgaa ggaactggaa gacccggaca ccctgctgat cgccagactg
    23581 ccgatggacg tcaagcggtt tgccgcgctg gccggactgt ttgagcgggg tgaggccgtc
    23641 gttaaggcca tgcctgccgg ttgccagtgg actgtaatta aaaaagcgag aggataaaat
    23701 caatgaccta cacgccggga ggaaaaggaa tcgcgccgcg gcagtctgag cggccatcgt
    23761 ggatcggact cgaagagatc gggcgcgacg cgctcaccgt cacctttaaa acaaaaaccg
    23821 gaacccgaac cgtaccgctg gccgatgttg aaaagtatga aaggcgcata ggatcaaagc
    23881 cgcatagcaa caaaggaaaa ctgaaatgaa aggcctgtcc gtcagcaaaa agtttccaac
    23941 atggcaccgt tcggctggga atcagccgac tatgtttttt gatcacatca tatccggaaa
    24001 aaagatccac acgatccgcg cgaacgccaa aggctattac aaagacggcg atgtcgttca
    24061 aattacacaa tggagcggtg cgccgtaccg ttcaaaggtt gagaaaacgg gtgtggtaat
    24121 ccggatcggg ctggagccgg tcacgctggc cctgctcgac aacggaacgc tgtcggccac
    24181 ggtcggcttt aaaactgttc tgccggtgcg cctggcgcag aatgacggcc ttccgctgga
    24241 gcggttcatc gactggtttt tcccgctgcg caagccgggg atttttaaag gaagcatcgt
    24301 ccacttttca tcattcagat attcgaaagg aaccaatgaa aaagcataaa attggctggc
    24361 tcaatgtgcc gggatatatc ccgcagacgt ggaatccgat tgtcggatgc tcgaaggtgt
    24421 ccgaaggttg ccgtaattgc tatgcggagc ggatggcaaa caggttggcg cggatgatga
    24481 attttgaata tatgacagca accggaggcg gaggaggatg gaacggagaa acgatcttgt
    24541 gcgaaccaac actcaaaaaa gcttttggct ggaagaaacc ccacgcgatc ttcggcaaca
    24601 gcatgggcga cctgtttcat gagaacacgc cggatgaatg gatcgatcgg gtattcgctg
    24661 tcatggctct ttgtccgcaa catctgttta tcgtgctgac taagcgggct gaacggatgg
    24721 agaaatattt ttccgagcag tggcggtctg cctatgtgga aggcatggcg cagaagattt
    24781 ataacgagcg caccggagaa gatccatcga tgtggcttgc tgttcatttt cctcttccca
    24841 acgtctggct tggtgtgacg gcggagaatc aggcggctgc ggatgaacgt attccgcacc
    24901 tgctccgcac cccggctgct gtccggtttg tgagctgtga gccgttgatt ggtccggtta
    24961 atgtatcttt atatcttcct ttcacaacta ggggtcgcgg cggggcgcat cgtgaaactg
    25021 gaggcctcga ctggatcatc tgcggcggag agtcagggcc gggagcgcgt ccgatgaatc
    25081 cggaatgggc gcgttcgctg cgcgatcagg cgcaggcggc tggcgttccg tttttcttca
    25141 agcagtgggg tagatttgcc ggaggctacc gagaactacc ggtgactcat agcgatgttc
    25201 tcgacggtca gcagcttcac cagtggccgg aggtgaagaa atgaataagc ccgaactggt
    25261 aattattacg gggctccccg gcaccgggaa gtcgacgctg gcgaagaatg aatttccaaa
    25321 tcacctgcac tatgaacccg atcacctgtt ctgcgattcg cgcggagctt accgcttcga
    25381 cgctcagctg tggagcttgg cgatcgactg ggttgtcaac atgactgact tcgcgctggc
    25441 ccgcggtgaa agcgtcgtgg tgtcggatgt gtttgaaaat atcgaagacc ttgagcggtt
    25501 taaaattctg tcgatctatc acggcgttga gctgcgcatg atcgaatgca aggaacggtt
    25561 cggctccatc caccacgttc cgcgctttgt gatgaatcga atggatgagc gtttgaaagg
    25621 tttaaaatga gcaaacttct gcgctgcccc tggtgccaca aaaccagcga tgacaaggat
    25681 aacgacaccc gcatggtcgc cgttgagctg gtcacgtttc cgcgtggcga tgagtcggcg
    25741 gagtattcgg tctgctgtaa ctggtgcgga gcgaccggcg cgatcgccga caccacggac
    25801 ggggcgctcg aaggatgggt cgctgaggat catctacaga tcctcgaaga gaaggccctg
    25861 cgcaatctgc gcgatgtggt gcgcgccgag atccgcgaac gcgaccgcaa gaccggattc
    25921 ttcgatgaag ccaaagcctg gacaaatatc cgcaacgcgc tgtcggcgtg ttaatcgcca
    25981 caccgccgaa ggcagaggcc cggaaaattc cgggcagcga agcggcaaaa aacacaaaaa
    26041 aaacagcaat gaaaaatcct gtcgatcctg taatcctgtc gaaaaaatcc cgctccgcct
    26101 accagcgcaa gtatcacgcc gagcatcttg aagaggctgc cgagcgcaag gagctggagc
    26161 ggtaccggcg cgaaaaggcc cgccggtact ggcgcacgaa gatcctgctg aagcgcgaaa
    26221 tgcccggact gatcgccgat gtgtcgcccg aaattctgga acaggagcgc ataaaataca
    26281 tggccctgct cgatgagccc tgcgaagtga tccatgtccc cgccgaagtt gtgagaaaaa
    26341 gaaaagtcac aacagaagaa gaagaggaag cggaagaaga aaactgaaac ttgaaacctg
    26401 aaacctgaat taagaccttc gactttagac ttttagattt tggactttta aaaatgacct
    26461 ttttgcttcc caacggaaac gagctgatga gctgcaagga gctggccgca gcgctaaagc
    26521 gtactccgca gtacgtctcg gccatgcgcc gcgacggctt catcatgccc ggcgaccgcg
    26581 ccaccgtgaa ccatgccctc atctggctcc atgaacaccc gcagttccgg cagaacaaaa
    26641 gacattgccg gttaccggtt gtcgattgct gattggatag attttcccga tctaagcccg
    26701 ccagcgtgcg ggctttttct ttgcgcccta tgcgttcttt cgcggctaaa tcgtatccga
    26761 ttaagtctgg ttcagtctgg ttaattctgg ttagtggaat aatgacgcgc gcgcgcgaat
    26821 gatgggggca tgggaaaacg accgcttcca tcaatcttca atcggcaatc ggcaatcgtt
    26881 aatgaattcg acgaacctat ccgggaagtg gtgtccggtt ttatcccgat gggaatgacc
    26941 cgcgtcgaaa ccgccgaaac gctcggcgta agcgtcgcca gcctgcgaac cttctgcgaa
    27001 aaaaccgata tccggtttcc tcgaaaccag cccggacggg ccgagcgtat ccgcgacacc
    27061 atgcgccgcg gaccccgcgc ccgccgtctg tcgctgaatg gccgcagcca gtgcctgtcg
    27121 gcctgggcgg aagaatacgg catccattac gaaacactcc gcaagcgcct cgaccgtggt
    27181 cgcactctcc gcgaagcgct ccgggtataa cgtatcgcag gcatcctgcc tgccttcagc
    27241 tgtcagacaa ggccggctgg aagccggcgg tacaccccgc ccgatctacc ggacacccgc
    27301 ctattctcgc ggaatgaatc cagcttcaat ctacggcgga ctctccgccg agcgttttta
    27361 taatctgccg aagtttccag ccggagccta cacgctgcgg gcgcggatcg tcggcgcgga
    27421 aacacccgtc gaaatcaccg ccgcgcaatg caccggcagc ggagctgact ggcgcgtact
    27481 gattcccgcc gcctcgctgg ccgcgctcgc caccggtgac tacaccctgc tcatcatagc
    27541 caccgactcc gccggcgctg aacagctcgc cgcgaaagaa accatcaagt tttacgcagc
    27601 cgcggaaacc gatctgcgca gcgacctgca taagatgctc gacgcgctca acgccacgct
    27661 ggcgaaaaag gccacccgcg atcaggcgag catgtcctac aacggacgat ccatttcacg
    27721 cctcagctgg gatgaactgc tcaaagcccg cgacgccatc gcccgccagg tgcgggccga
    27781 agaaaacagc ctcgccggtc gctcacgaat ccaaaccgtt aaaatgaggt tcatagaatg
    27841 aaggctttag gctttgggct tttgactttt ggccgagatg ccgagacggt agctccgtcg
    27901 gtcttgcgcg aatggatccc gcctaaatcg gaaatagtca atccgaaatc ggaaatgtcc
    27961 cgcaactacg ccttcggccg gccatcgcgc accgctgatg atttcttttc cggctcgttg
    28021 tccgcagatc aggccgtatt cggatcgctc tacgccatgc gcaatcgcgc ccggcagttg
    28081 gagcgcgacg actcgcacgt ccgcaagttc ctgatcatgg cgaaaacctc catcatcgga
    28141 gcaaaaggaa tacgcctgca atccaaggcc ggcgacttta tcaaaggcca gttttatcca
    28201 agccgattcg atcaggattt gattgaaacc gccttcgaag atttttctaa gtcatggaat
    28261 ttttcaatcg acggaaagct cgaccgacgc cgcttcgcgc aggttgccat tcagcgcgtc
    28321 ctggtcgatg gcgagtgtat catccgccgg caccgcggcg ctggaatgta cggaatccaa
    28381 aaccagctca ttgatgctga actgctcgac cacacgctta accagccagc ctcatcgaac
    28441 cgcaacgaaa tccgcatggg agtggaaatc gacgcgaacg gcaagccgct ggcctaccac
    28501 tttcttgaag aagcccctcc ggcttggatg ggaaccgctg tatccaccgc gaaccgcaag
    28561 cgcattccgg cgaacgacat caatcatatt ttcattcaag agcgccccgg ccagacgcgc
    28621 ggcgtcactt ggctggcccc gactggtctg cgggcgcgga tgctcgacgg aatcgaaacg
    28681 gccgtcaccg tcggctaccg cgtcgccgcc agcaaaatgg ggtttttccg tccgggagaa
    28741 aactacgaag gcgaagagat cgaaacggcc gatattccga gcgatgttac acccggagag
    28801 cttaacctgc tgccgaaggg tattgaattc cagtcgttcg acgccggata tccaaacgct
    28861 gaatttgacg gtttcaaaaa atcaatcgtc cgcgaaatcg ctggcggcct cggcgtatcg
    28921 tatccggagc ttggaaacga cttcggcggc gtatcctact ccgcaggaca gatcggcgta
    28981 cactccgacg ccgcgctttg gtcggatctc cagcagttct ggatggactc attcgaagaa
    29041 ccgaacttcc gcgaattcct cctgatgtcc attacatcag gagagctaca gcttcccctt
    29101 tcaaaactgc ggaaatttca gaacgtccgc ttccagcctc agcgccgtaa acatatcgac
    29161 ccgctgaaaa cgcataatgc acagcgcatt gccctcggcg acatgagccg cagcccgttc
    29221 gacatcgccg ccgaaaacgg agctgacttc gaagacgtga tcgacgaaac aaaagccgcc
    29281 atcgaaatgc taaaagccgc cggccttcct gtccctgaaa gctgggcgtc cggaaaggct
    29341 ctatcaacca tcatcgaaga accacctgcg acctaatcta ccggacagcg aaataggata
    29401 tttccatgaa tgaactcaaa aaaatccttg cacgaatcgc tgaagaaggc ggccagctta
    29461 accgtgcggc catcatcaag ccggtgctgg tcaacccgac acgcgcagaa ggtgaagatg
    29521 ctcagtaccg ctttgaactt tcgttcagct ccgagactga aaaagttaaa cggtggtttg
    29581 ggacagaaat tcttggtcat cagcccggag aaatccgttt agagcggctt cagtccggaa
    29641 accatcctct tcttctcatg cacgaccatg atcgccaaat tggcgtaatc gaatctgtaa
    29701 aacttgagaa cagccgcgga acggctgttg tgcgctttgg gaaaagcgcg ctcgctcagg
    29761 aaatcagaca ggatgtcatt gatggcatcc ggtccaatgt gtctgttggc taccatatac
    29821 atgacatgaa gctcattaaa gagtcagacg aagagggtga tacctaccgt gtgaccgatt
    29881 gggctccgta tgaaatatcc atcgttcctg tgcctgccga tgtggatgtc gggattgggc
    29941 gcagcgctga actgaatcac cccaaaatta accaggagaa gaaaatgact gaactcgaaa
    30001 aacgcgctct ggcgatgggc ctgcgcaaag atgcttctga agaatctgtc cgtgaagccg
    30061 aactcgcaca cgcccgtaaa gaaggcgaac gcgccgctaa agaaaaaggc gatgcagaac
    30121 tcgcccgtca gaacggcatc cgcgaactgg ccgtcgctca ccgcgaacgg ctgatcgacg
    30181 ccgacacgat ggccgaaaaa gccatcaaag acggaaccac cctcgacgct ttccgcagcg
    30241 aaatcctcgt cgcctacaaa ggcaaatccc ctgcccttaa atcctcgcgg gctgacggcg
    30301 accagcgcaa gctgctcgaa ggattcagcc tgcgcaaatt catgcttggc gcagtcaccg
    30361 gtgaaagcct caagggtgcc gaagccgaag tccagtccga aggccagcgc gaagccagcg
    30421 cccgcggtat ggatgtcggc cccggctaca ttcccgccat ggccatcggc gaaatcgcca
    30481 aacgcgccga actgatccgc gacggccaga acgccacaac gaacgccgac ggcaagtacc
    30541 tggtcgccac tgaaaacatg ggtttgatcg gtgctctcag cccgcgcctg tggatgaaac
    30601 agcttggcgt gaccatgctg tcaggtttgg tgaataacat cacactgccg accatcgagg
    30661 ccgaaggcga agcggttgac cgcacggaaa tcgaatccga cagctctgtc gacgtaacgc
    30721 tcggacagcg tacgctcagc ccgacccgta tcccggcttc ctgcacattc agttcgcagc
    30781 tgttgcgcca gaccagcccg cagatcgaca gcgtgctcag caacatcctg ctcagccgca
    30841 tcgcccgccg ctggaacgcg aaagggattg aattcctgct cgctctgtcc ggtaccggct
    30901 ccgttgtcac cagcggcgac gtgctcactc acgccatcat gcgcggcttc ctgaccaagc
    30961 tcggtatgga aaatgccgac gaagctcccg gcgcgttcct gatcaatccg gatgtcgaag
    31021 gcgttctggc cgcaacgaaa gttgatgccg gatcgggcct gttcctgcac acggaaaagg
    31081 aaaacggcga aggccgcatc ctcaaccgcc gttctctcac cacttcgctg gttccgaacg
    31141 atctcggcga tgcaaacgac ctgagcgcaa taatcttcgg cgcattcccg cgcctgtgga
    31201 tgggcgactg gggcggcgtt gacttgatcc gcgacaacgt gaccaaagcg gccaccggac
    31261 agctcgttct caccgcggcc agcttcatcg gcttcgcggc tgaaagcccc aagtacttcg
    31321 tcaaagcagt tgacgttgac ccggcggctg tcgccggata accggatttc cgattgctga
    31381 tttccgattg ccgaatggcg gtcggagtca gcagccggaa aataaactga actgaaaaaa
    31441 aagctgaaaa ggagcccatg aaaaacgtaa tcgcaatcat cgccgtcgca ctgatcgcag
    31501 tgtcagccgt caaggctcag aactaccaaa cgatcgacat ggccgcatac ggaacaacgt
    31561 acacggcaac taacgcgcag tcgttcggcc agacgctcta cacgtccgcg gcaatcacct
    31621 acgacagcgc aaacatcacc aactccatca cattctacgc gaagatcgac gggatcaact
    31681 atctgctcgg aaccagctcg gttacgaacg gacagtatga cttcattgat ctgaacccgc
    31741 tcgccctgcg gccaggttcg gcgctggtga taaataccac gcagacgaac gtaaacattc
    31801 gtctgggtaa ataaccgcct ccaccgtgca cccaaacggg gcggcttcac ctccgccccg
    31861 tttttctaat ggcgaccagc atttcgcata cggagaaaaa atgaaaataa aaatcacgaa
    31921 tgaagtcgtc atgagtggcg ggtggtttct tccaggtgcg gttattgaaa tcgatgacgc
    31981 gaaggccgct attttaattt cagaaggcaa ggcgctcgcc cattcggaag ttttaaaaat
    32041 tgctgtaaaa tcaggcgctt ttgcgtggat aaaaaaactg gtgaaaaaat gaaaaaaaca
    32101 tccgccagat ccgtcttatc cgccatctgc attctgctct tcgccctatc ctcccccgcc
    32161 gccgtccgac cgatctccac cgcctgggac attcagaacg gcaacaagcg cctggtggac
    32221 atcaactttt ttcaggccga aagcgttcgg ctcaccctgc aaactacgga tggagccaac
    32281 gcctaccgac tcaccaactc gaatttgatc gttgtgtggg aaatcaccgg atggaacgat
    32341 tacaccaacg tctattcaat cgctaccggc actgtaatat ccgccaccaa tggaatttct
    32401 tcatacagtc taacacctgc gcaagccaac ctctcacccg gaacataccg cggctacgtc
    32461 cgcgcattac agaccgtcag caatcagctt gatcaggtcg ccgtcctcgc ttggcagcgc
    32521 ataaaagttg aatactctcc agactcgcgc aactacaaca tcgtcggtcc tctgacatat
    32581 cctccgatct ataccgaggc ggacttggaa gcgatcacga atcagattgg tagtttgcag
    32641 tctgaggttg tcagtttaag caaccgggtt gacgccgccg aatcggcaat caccaatctg
    32701 caatctcaaa tgtccaccgt aaccggctgg ggcgaccatg ccgggcttta tccgctcatg
    32761 gcagcgtgga acgcgacgaa ctctgcgctt tggaacgcga tggggaaccg gctggaaacg
    32821 tcggtattta ccggctggac gaatgcgcag cactctatca acaccaatct ggccgcgatt
    32881 gtaacgctgg cgacgaacga tatttccgga ctgaaatccg gaaccggcgt atggaatagc
    32941 gtgaccggca aggtggatcg aaccgatgcg acctataccg gaacagtcgc tacggcggcg
    33001 gcggcgtttg gctggggcga tcatgccttg gctgggtatc tgacatcggc acctccgcaa
    33061 acggtaacca gcgtgaacgg tttgaccggc gcggtggtta tcacggcggc atcaatcgga
    33121 gcggcgacgg gcactctgct ttctgtttcg aacggcaccg cgacgaatct taccgttgtc
    33181 gatacgctcg aagttaaggc acaggctgtg tctcaatccg gcctcttatc agggtctcaa
    33241 cttccggcag agttcgcagg ggagtacact tactgtggaa cagagcagat gtacatgtcg
    33301 ggtatgcaac tattcaccaa ctcgtttgga aacttcatgt tcattgaaaa tggatatttc
    33361 tgcggacagt atggttcttg gacgatcggc ccgtcacagg gcggcgtgcc gtacggcagc
    33421 tcaaccaact gctactcttt aattgatggg gtatggtatc agttcgacgc gaatgaaggg
    33481 ccgattgcaa ccaccgacat acagacggtt cggcttcctt tttattcggc atcgaacggc
    33541 aacgtacgcg ccgaaaatct ggtatcagga aaagggttac tgttaaatgc ggattattcg
    33601 gtaattgaca acaaacccgg cggaatcggg atgg
//
LOCUS       DAOPZO010000008        32055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_1317_length_32055_cov_8.529281, whole genome shotgun sequence.
ACCESSION   DAOPZO010000008 DAOPZO010000000
VERSION     DAOPZO010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 32055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32055
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1317_length_32055_cov_8.529281"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..838)
                     /locus_tag="PKI68_01225"
     CDS             complement(164..838)
                     /locus_tag="PKI68_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNX52345.1"
                     /translation="MSIRENLEAVETRIAAACEKAGRRRDEIKLVAVSKTKPADAVLE
                     AADAGQILFGENRVQEAQAKIPLCPGHLQWHLIGHLQSNKARIAASGIFRMIHSVDSE
                     HLLRELDKYAAVPLPVLIQVNVSGEGSKDGCAPEETAALIKAANQCGKVEVHGLMTIP
                     PFTPDPEKARIHFSNLRELRDRLQQETGTPLPELSMGMSHDFEVAIEEGATYIRVGSD
                     IFGGRS"
     gene            complement(835..1923)
                     /gene="holA"
                     /locus_tag="PKI68_01230"
     CDS             complement(835..1923)
                     /gene="holA"
                     /locus_tag="PKI68_01230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNX52346.1"
                     /translation="MAVKLIHGTDEYLVSHNARKTVNALCPENEQTLSLETVDGDVKT
                     IDEAVTALKTCIGALRTVGLFGGQKTVWLRDVSIFKDAVISKNETVKGLLGKLADDIK
                     KGLPDSQHLIISAPGVDRRSAFYKICEAAGAVEAYDLPEQDYKKKPVIRERAQALFAK
                     APCRIGAGALDLFLEKVGTDTRQLVMEVEKLTLYIGDRKEITVEDVKAITSSSAEAVA
                     WDFTDALGERRLDEALKILRQLIFQGEAPVGIIFAIESLYQNLMQYRAYIDQGWLRLS
                     GGYGANAVQWSSDQEMEKMFSQLPSDPRETHWFRTSKLAQQAKNYSAEELRRCQKRLL
                     ETHEQLVSSGVPQELILEMLVIKLIAKV"
     gene            2001..2483
                     /locus_tag="PKI68_01235"
     CDS             2001..2483
                     /locus_tag="PKI68_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNX52347.1"
                     /translation="MNRPLWAPWRVEYIHSEKNGECFLCRMFRENTDRKNLLLVRGKT
                     CAVMLNRYPYNSGHLMVAPYRHIAEPAGLTPEENLEMAALTARAVDVLRTVMSPHGFN
                     IGINLGEAAGAGLKDHLHQHIVPRWVGDTNFMPVLGGPRVIPEALEITYDLLLKSFNR
                     "
     gene            2496..3395
                     /locus_tag="PKI68_01240"
     CDS             2496..3395
                     /locus_tag="PKI68_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNX52348.1"
                     /translation="MPKNIPVLCVEAETLAEAYEKALTALYTRGTRFKTQYDKPGDPL
                     SLDCTINITVLDPMKDPMIHKAFPGGIADLREYVMELKGFKDHWVKNMNDPADTRWEY
                     TYHGRLADYGVWKELQNGKSAEAGPFKIRQLDGIIEKLSKQPFTRQAQAITWMPNLDL
                     ECYDPPCLQSIWYRILEDEDGVWWLNTNIRFRSNDAWGANFMNMFGFTLFSKEVIADA
                     VAAKTGKTVKLGRLNWQADSFHIYGKDIQSAKERLFDRLGTTDFEDRVYEFHDEMIRE
                     MYDEAEAGVLAKIKTYDAAHARP"
     gene            3678..5354
                     /gene="ettA"
                     /locus_tag="PKI68_01245"
     CDS             3678..5354
                     /gene="ettA"
                     /locus_tag="PKI68_01245"
                     /inference="COORDINATES: protein motif:HMM:NF008775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNX52349.1"
                     /translation="MAGEYVYNLQNLTKQHNKKTVLEDVTLAFFFGAKIGVIGGNGSG
                     KSSLLRIMAGVDTEYMGQVQIAKRARIGYLPQEPQLDNSKTVMGNVMEGVAASQAILD
                     RYDEIWELLAGDVSDEESEKLNEEVALLQDKIDTQDLWNLDNQVEMAMDSLRLPPGDS
                     SVEKLSGGEKRRVALCRLLISNPDVLLLDEPTNHLDAESVAWLEEYLKRYTGTLVVIT
                     HDRYFLNNVTEWILELDAGRAFPYKGNYEAWLSQKQAMLDAQSKQAASRTKLLARELE
                     WIRTNPSGRRAKSRARIQNYEQLAKQEIDTREDNVEIQIPSGERLGNLVVRGENLTKC
                     YGDRVIFENVSFDLPPGGIVGIIGANGAGKTTLFRMITGAEEPTGGKLTVGPTVNLSY
                     VDQSRDALNPDSTVYEEICGGNEWIDLGGKRMNGRAYCGKFNFKGAEQQKKVGVLSGG
                     ERNRVHMAKLLRRAGNLLLLDEPTNDLDVTTLRALEEALLSFGGCAVVVSHDRWFLDR
                     IATHILAFEGDSKVTWFEGNYEAYHEQRRRLLGDEADKPHRIKFHPVSHK"
     gene            5366..6289
                     /locus_tag="PKI68_01250"
     CDS             5366..6289
                     /locus_tag="PKI68_01250"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNX52350.1"
                     /translation="METLVTRTLELTVDGRDRKCPVYVPGALNAAQPVPLLLVFHGAE
                     GTGASMIKKFRALADRNGFLIAAPDGIGGRWNALQPDEIAATGNADEPAFAAAILNAL
                     EKEYSVDSSRIYASGFSNGGALCQVLASRPESRLAAIACVGATLPAVLQNQIVSAPSV
                     SVLLLTGSDDEFFGAGGSKLGPFLSADKTARLWAEHNKCSLEHFTIQMPQPTPASGHP
                     SEGGELLKSPPSEGCRNGGVGFPAFHTTALQPLNSLTAPARWINEKTGVEVQSRWIEG
                     GKHSWRLSADLDTSLTIWQFLSAHSRYTQNS"
     gene            6374..7318
                     /locus_tag="PKI68_01255"
     CDS             6374..7318
                     /locus_tag="PKI68_01255"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNX52351.1"
                     /translation="MAKLENTFSWSFSAAADFDECRRRRYWSKYGMWGGWEKTAAPET
                     KKAYQLNKMDNRWGLMGQAAENSVMRILRQHQAGNPITADEAFETVARPFLRQKWTES
                     KRGEWKNDPKKFCCLREHYYGTMGDEKAAAEQMSGQIKNCIANFIEKVLPRIGAVTRE
                     QELPVKTPDQGGDPENMEFKGVKMYVIPDYAYRIGGQFHIHDWKAGKVKESHREQLGL
                     YALWAREKFRAKPEDTFLYVEYLNEGVVAPFQLVPEDFVRLEARVEDSVAEMTEYLAG
                     FDRKRNEPLPREEWELAADLTSCKNCNFYELCKPELGE"
     gene            complement(7324..8181)
                     /locus_tag="PKI68_01260"
     CDS             complement(7324..8181)
                     /locus_tag="PKI68_01260"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNX52352.1"
                     /translation="MKKILFSLFALTFAVYAQESATNTVQETVKVKMTGDRVSLRAAP
                     ETNAVLLGRAMLGDELTLRDNSNPDWVGVQPPDTVDLWVNSEFVISNTVVPELLNIRS
                     GPSLSHGTVGTAHKGDILTVRSKIAQWLKIAPTSNTVIWVSRKYTAVPGNAFAGPDAV
                     PAAVQETQTVVQAFSEPTVADVLIAASASSATNMPNKLKIDPAKPQGVTGTYYGILQP
                     SDNMLYKLVDDHFTDIIVCHVYGNKPQMLTFTGMKIEITGKAYWVEGKDLPVVIPARI
                     RLMPTVTNG"
     gene            complement(8178..8969)
                     /locus_tag="PKI68_01265"
     CDS             complement(8178..8969)
                     /locus_tag="PKI68_01265"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNX52353.1"
                     /translation="MFFDTHVHFDDFVKDGTLEGLLERAETAAVSKMIAVGGSPEANE
                     LAQMLAEAFAERIYAAAGYDRHMADSICDISALRELAARPSTVAIGETGLDYFYEPGK
                     AKEQQRLFFQCLETAIHTRKPVIVHTRDADDDTLSILTDYAKHWKGDAARAGVIHCFT
                     RDRKMAAALLDLGFYISFSGIVTFANADPLREVAKFVPDDRLLIETDAPYLAPVPHRG
                     ERCEPAFAVDTAKRLAELRGCTVESLAKTTAQNATRLFGLKEEPL"
     gene            9108..9443
                     /locus_tag="PKI68_01270"
     CDS             9108..9443
                     /locus_tag="PKI68_01270"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017002499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNX52354.1"
                     /translation="MNALNSQTCEACQANAPQLTAEEINELLKQLQNWKLIEADGEKR
                     LERKFKFDNFAKAIAFTDRVGALAEEQNHHPALLTEWGKVTVTWWTHKIRGLHKNDFI
                     MAAKTDELF"
     gene            9460..10209
                     /locus_tag="PKI68_01275"
     CDS             9460..10209
                     /locus_tag="PKI68_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNX52355.1"
                     /translation="MIDIIKADQRHFSDFHWLKTYWLFSFSDYFDPHNIQFGALRVFN
                     DDVVEPGKGFPTHPHNEMEIITIVLDGEMTHEDSMGNKTVIRCGDVQRMSAGTGLTHS
                     EFNLAEEPVHFYQIWIFPDQAKLTPSYDQRNFKPEQWKNCLLPVASGRNIPGTVTFFT
                     DATIYRSALDAGKKVSFEKTAGRRIFVYVTAGQLEINGTHLDANDQARIDEDGSLTIK
                     AHKAAEFILIDVPSCKGWGYSEKILHGASRR"
     gene            complement(10212..>10779)
                     /locus_tag="PKI68_01280"
     CDS             complement(10212..>10779)
                     /locus_tag="PKI68_01280"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNX52356.1"
                     /translation="LMILIIDNYDSFTYNLVQYLGELGAEMKIFRNDKITVAEAVALR
                     PEKVLVSPGPCSPKEAGVSCDIIREFGPRVPLLGVCLGHQAVGDVFGGNVVRAARLMH
                     GKTSPILHKGESVFKGLPSPFNATRYHSLLVERESLPSCLKITAETAEGEIMGLMHTE
                     YPVHGVQFHPESILTEHGKQLLQNFLDL"
     assembly_gap    10780..10879
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10880..12381)
                     /gene="trpE"
                     /locus_tag="PKI68_01285"
     CDS             complement(<10880..12381)
                     /gene="trpE"
                     /locus_tag="PKI68_01285"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNX52357.1"
                     /translation="MSVQPNRAEFLKKAMQGNLIPVWKEILADQETPVSAYERVRKFL
                     REKDHASHTWMLESVEGGEHIGRYSFIGGNPRAIVRARGAVTEITEEGTTTIIKNTDP
                     LNVLKACMSRYQPVREPALPRFTGGAVGFLGYDMISVFEPRVPVIKADIIGNPDMVMM
                     ITNSIIIFDRVKHTMKVVSNAYVSGDPDKAYDEALAEIDELCQALLQPVSRVLIDAHL
                     DVEQVVPVSNMTVPEYREMVEKAKEYIRAGDIIQTVLSQRFEVENAADSLDVYRALRA
                     INPSPYMFCLDLGESALVGSSPEVHVRCEDRQVELRPIAGTRPRGKTEAEDLALEKEL
                     LADPKELAEHVMLVDLGRNDVGRVCEFSSVRVPEQMVIERYSHVMHIVSDVTGTLSPE
                     YDAYDVMRATFPAGTVSGAPKIRAMEIIAELEKTKRGPYAGAVGYFSFDGNLDSCITI
                     RTVVLDKNKAYVQAGGGIVADSVPETEYQETRNKAGAVLKALSLAKHYAAA"
     gene            12516..13763
                     /locus_tag="PKI68_01290"
     CDS             12516..13763
                     /locus_tag="PKI68_01290"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HNX52358.1"
                     /translation="MVSASTKSGFSIKQFLSFLGPGFLVTVGFIDPGNWATNIQGGSQ
                     FGYRLLWVITLSTLMLIVIQHMAAKLGIATGKSLAANIRERFPAPVSAFLGVTIVLAC
                     VATDVAELIGGAIGFNLLFGLPLWAGALITVFLEVFLLISQRYHRLETIIVGFLGIIA
                     CCYVIEILIVKPDWAVLAPALVIPHVDRNSIYVAMAILGAVVMPHNVFLHSNVIHSRK
                     WGISEAEKMTLLRYEKYDTLAAMLLGWVVNSAMIIVAAAVFARHQVAVTGIEQASETL
                     RPLAGPLAGLLFAVALVFAGVGSSITSSMAEANVITGFLGKPEDPRTLLYRVSVFITA
                     IPSFIIILLGMDTFKILIFSQVVLSIQLPFTLIPLLILCRSRKVMGAFRSGRREFITA
                     TVIAAVIIALNIYLLYSTLAGGT"
     gene            13765..14169
                     /locus_tag="PKI68_01295"
     CDS             13765..14169
                     /locus_tag="PKI68_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNX52359.1"
                     /translation="MQVYKNILVTLDCSPVDDAIINHVVLLAVQNRAKVHLLHVVHAH
                     TLDQRRALRAEAGPCLEKHAGTLREQGVDAHKIIRDGEPDEQILKEIETGSYDLVAMA
                     THGHKFFGDILFGSVSKSLKHKIGIPLLLLKP"
     gene            complement(14159..15424)
                     /locus_tag="PKI68_01300"
     CDS             complement(14159..15424)
                     /locus_tag="PKI68_01300"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNX52360.1"
                     /translation="MKSNEWFKRALKVIPGGVNSPVRAFNAVGGGPVYVTKGRGARIT
                     TAEGRELIDFCGSWGPLILGHARPEVVDAACAAARDGMTFGINTPREVEFAELLCAQV
                     PSMEQTRLVNSGTEAVMTAVRLARGFTGRRRIIKFAGGYHGHSDGLLVGAGSGLLTGG
                     ISSSAGVYVNEGDVFLPNYNDKEAVSRIVNECGDELAAIIVEPVAGNMGLVLPRDGFL
                     EHLRAEADRCGALLIFDEVITGFRFGPTSYGTLKGIAPDLTCLGKIIGGGMPLAAFGG
                     RRDVMQRLSPVGNVYQAGTLSGNPVAVAAGMKTVELLIKENPYPRLAEAGAKMSAKLN
                     QIAGEFHVAQSGGVFTPFFRRDPVRNLDDAKSCDTSAYAAFFRSMLERGFYLPPAQFE
                     AAFISAAHTEHDLDQFAAAAQAVSDGIMV"
     gene            complement(15429..16409)
                     /gene="hemB"
                     /locus_tag="PKI68_01305"
     CDS             complement(15429..16409)
                     /gene="hemB"
                     /locus_tag="PKI68_01305"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNX52361.1"
                     /translation="MFPEIRLRRLRRTEGLRRMLGQPLPGPEKFIWPVFIREGKGVDE
                     AISAMPGQSRMSVDVLLKRLKPVAESGVGGVLLFGLTDPAKKDANGSEAFNEKGAVQK
                     AIPAIKKAFPQLTVFTDVCLCAYTDHGHCGPLNAAGEVDNDAALTLLQKVAVSHAQAG
                     ADCVAPSAMMDGQVLAIREALDEEELTDTLLMSYSSKFASAMYGPFREAEQSAPGKGD
                     RQSYQASYSDLRTALRESEFDEDEGADILMVKPAMMYLDVIHQLRETTDLPVAAYNVS
                     GEYSMLIAQAERGWGDLRAMVRENMAAMQRAGTGIFISYWASRYNELLKD"
     gene            complement(16399..18495)
                     /locus_tag="PKI68_01310"
     CDS             complement(16399..18495)
                     /locus_tag="PKI68_01310"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNX52362.1"
                     /translation="MNGSLKVFRAGTRNSRLALWQTARVLEILRKFLPEHEFQTVEIT
                     APGDADRTTDLRESPADFFTRTLDEALLAGKIDFAVHSAKDLPEPVPEGIDWFWLPDP
                     GDRRDVLVGSLNPKVIGVSSDRRAAYAAKRFPFAKQYPVRGNIEERIQQLDAGKFDLL
                     IMAGVALQRLGLEDRITEWIPLDQLETSESQGALAVTFRKNDPRLCAIRSLFVKSATF
                     VGAGVCAGHCTLDGLHALRTAEVCLYDALMDESLLHHLPETARKVYVGKRLGAHSQSQ
                     DEINRLLCDYVRKGLRTVRLKGGDAGIFGRLAEEMDALEALGLAGRVLPGISAMQTAA
                     AHTGILLTRRGVARGFSVMTPRLQGGGIGPVTKAARAELPVVFYMAAGTAEKLAQEMI
                     ADGVPPAMPCALVFGAGSEKEAVFRSTLGNLPAVDEVTGRLPGLFIVGEIARYGFDRS
                     LGALGGQRVLLTCSEALMEKAVQAVNDSGGRPVARPLIRLKVSTDGLDAVRHLASYDW
                     IALTSPSAVRCFHELLLKEKIDLRRIPKIMSVGSGSLWALRRIGLGCDLMPESDFSGA
                     GLLADAVPVVKGQKILRLRSQNAGTELADGLRAAGAEVTDCVLYTNKPVHYPEMPEFD
                     SVYFASASAVESFIEQWGAAVLKNKIILAIGGPTQAALKSFGLNAGVIGEMATVEESL
                     RALAVYEINRRITHVS"
     gene            complement(18452..19942)
                     /locus_tag="PKI68_01315"
     CDS             complement(18452..19942)
                     /locus_tag="PKI68_01315"
                     /inference="COORDINATES: protein motif:HMM:NF017051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX52363.1"
                     /translation="MSESQKTSALPVNLLLADRPCLVVGAGRIASRKIGYLLEAGAKV
                     TVVSPEVSEEAAAWISEQRIVHLNRPFKSDDTAGFAVVFAATNDRYVNRLVLDDCRKH
                     KVLCSCVDGHWAQSDFTTPAISRHGNLTLTVSTDGHSCRQAKLIKDNLAKHIAMVETA
                     DLVVVGTDHHHMDIEEREPFHLTGPRFERIGWMIMQLWGIHEFMILNTCNRVELIAVA
                     SRETSTNGILRHLLGFDKLTEDKFYLKHGAEAYGHLCMVSAGMLSQTPGESHVAAQIK
                     DALAEAQRRGWAGRMMQEWVSTLLHVSKHIQTEVAPLFCSEEIEDIALKYLQTQIPGL
                     SSKTVMVLGAGRIGEALVRKAVQKTGKIIWCCHINRPEIPGEWKDRVELCTFNDFMNR
                     LEETDVIISSAGAPGHVLHRGHAPFFNRQKPVLLIDLGMPRNIEPALDGLVPGLRIVD
                     LDGLKCRYRHGTAETNKIYHRCQGIIEEHQDQYERIIKSFQGGNAQ"
     gene            complement(19935..20936)
                     /locus_tag="PKI68_01320"
     CDS             complement(19935..20936)
                     /locus_tag="PKI68_01320"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52364.1"
                     /translation="MTGYSSSEKKVLTKLQRGIEPVSRPFRNLDIPEDAVPALLRRTK
                     EAGLLRRFGGIFDARRLGYKSILCALDVSAEQIEEKAAIIGAHPGVTHCYERLPLYGR
                     GNYPRLWFTLAMLHDEFDQGLAALRAQIAPCTLFELPALRRFKIDVVFDLQTRNRDEV
                     FPGLDPSAVPSQDETFRTFSADEKALVRVLDQQIPLTERPFAALAEMIGQTEENILST
                     LKIWKEQGVLRRIGAILYHREAGFKANAMCVWPVDGDIAAAGRRTAARPEVTHCYQRP
                     RLETFPFDLYAMIHTGSWDETKALYKDISFTCDLQNGELFASGREFKKDSMKYFNHE"
     gene            complement(20933..21889)
                     /locus_tag="PKI68_01325"
     CDS             complement(20933..21889)
                     /locus_tag="PKI68_01325"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX52365.1"
                     /translation="MHKPGRNPSIIAWEITRRCGLNCRHCRGAARDCDYAGEFSTAEC
                     LKTVDALAAFSKPMIILTGGEPLMRADIFDVARYAAQKGCRVVLATCGHRLTPDVAAE
                     LRTSGVMAVSVSLDAATAQAHNAFRGVEEAYEKTLNGLNHLKAAGVPFQINTTVSKLN
                     VDELPALLEQAVTLGAATIDFFFLVPTGRGAQMTDLALSPAARDRALEWIATQAKSAP
                     VRVKTTCAPQYKKFISSEHTPPFMGCMGGRGFIFISHTGILQPCGFLDLPCGDLHAAG
                     FDFETLCHTSDVFKNMRTLNPFAGCPARAYARTGDYMKEEAE"
     gene            complement(21882..23054)
                     /locus_tag="PKI68_01330"
     CDS             complement(21882..23054)
                     /locus_tag="PKI68_01330"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX52366.1"
                     /translation="MLGISKLYLDNVESSDELRYGLTRRPASVRRPIVVWNCTQFCNL
                     ECAHCYARSDATTCADELTTAQAKKMIDEIASFGCPVLLFSGGEPLVRKDVPELAEYA
                     RAKGLRAVISTNGTLIDRPLAGRLQKIGLSYVGISIDGTETTHDLFRCKKGAFQAALR
                     GIRNCRDAGLKVGLRFTLVKDNIADIPAVFDLMEAENIPRICFYHLVNTGRGEDLNND
                     DGMPSHEATRAALELIIKRTAELHAKGKKVEVLTVDNHSDGPWLLRKMRAENHPDAAH
                     VYDLLKLNGGAASGSGVGISCISWNGDVYPDQFWRSKKLGNVLKTPFGEIWTDPENEF
                     LQKLKKKAAHVTGRCADCQYLELCGGNFRARAEASTGDLWAPDPACYLTDEEIHGA"
     gene            23353..24378
                     /locus_tag="PKI68_01335"
     CDS             23353..24378
                     /locus_tag="PKI68_01335"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF009395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNX52367.1"
                     /translation="MKPICKTENLRVESVTKLITPGELKKTLPLTEASKETVVRGREA
                     VCAVLERRDPRLLVITGPCSIHDPAAALDYARRLKALSEEVKDRFLIVMRVYFEKPRT
                     TIGWKGFINDPHLDDSCDMEYGLREARKLLLSITGLGLPVATEFLDPIIPQYTADLVS
                     WAAIGARTIESQTHREMTSGLSMPVGLKNATNGSVQSAVDALLACRSPHSFLGIDQDG
                     RSCIVKTRGNPDTHLVLRGGGGKTNYHADEVAEAAAKLRAAGLPEMIMVDCSHANCAK
                     VAVRQAEVWKDLLEQRKKPDCPVMGAMLESFIEEGNQPIRAGLKYGLSITDPCIDWNM
                     TEQLLRM"
     gene            complement(24421..25533)
                     /locus_tag="PKI68_01340"
     CDS             complement(24421..25533)
                     /locus_tag="PKI68_01340"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNX52368.1"
                     /translation="MNPVKIAEQFVVSGQLASIRPTGSGNVNDTYLAVFRTHFSEERI
                     IIQCISQRVFHHPEWIMENLSILTSHCHKQLQAESDRADRIWQLPRIVPCRNGQDYFK
                     DENGDYWRALTLIASATSFDTAQSAEHAFEVGTVLGQFHRLVSNMNPFSLRDTLPGFH
                     ETPKYLEKYDAVVRTPEAQELIKRSLEVRNLQHFVEARRKFAYVLQNALDAGELTLRL
                     IHGDPKVNNIMIDDDTGKGTAIIDLDTAKPGLIHYDFGDALRSLCNQEGEETDELGKV
                     SFDLDLCEAFVRGYIVYAKEFLTENDKKYLYDSIRLITFELGLRFFQDYLAGNVYFKV
                     KSPEQNLQRAQIQFRLCESIETRRRQIQELLTQAFV"
     gene            complement(25641..26759)
                     /locus_tag="PKI68_01345"
     CDS             complement(25641..26759)
                     /locus_tag="PKI68_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNX52369.1"
                     /translation="MKIKFLLLLLFPGLVACTAAPPAAKLSLEQKIGGILMVGFRGMS
                     VDGNSPVVRDIRDYNLGGVILFDADLELKTNERNILSPEQLAQLTGTLQSFAKNRLLI
                     AIDQEGGRVNRLKPAYGFPPTISQQQLGERNDPEFTYRQARQLASALKAAGINLNLAP
                     VVDLAVNPDNFIVKKERTFSADPAQTIRNAGQFIRAHHDQGVMCTLKHFPGHGSSMAD
                     SHKDMTDVTATWSGAELEPYRQLCKETDVVMTAHIFQRHLDPELPATLSNRIITGLLR
                     NQIGYDGVVMTDDLGMKAIADHFGFEAALEYSLNAGADILLIANNETYDPDLVPKTVQ
                     IVAELVRSGRVPESRIDEALRRVSFFKAKLNQSPPADR"
     gene            26874..27884
                     /locus_tag="PKI68_01350"
     CDS             26874..27884
                     /locus_tag="PKI68_01350"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNX52370.1"
                     /translation="MKRLPAEWEPQDAVLICWPHEKTDWAPYLNEAEAVFMDIAQAVA
                     RFEKLIVVVPDETCIHTKLAARGVNMANVALYEIESNDTWARDFGPITVYENGQPVLL
                     DFTFTGWGGKFEAGLDNQITAKLHAAGAFGTTPLRSVDLILEGGSIESDGAGTLLTTA
                     ECLLNPNRNAHLDKRQVEVLLSKELGFKNFHWLENGWLAGDDTDAHIDTLARLCPDNT
                     VLYVRCDDPADEHFPALRNMEDELQKLPAYRLLPLPWPTAKFDEDGNRLPATYANFLI
                     INGAVLVPTYNDPAKDAEALATVKKAFPDREIIGIDCSALILQHGSLHCVTMQIPEGV
                     LK"
     gene            27881..28771
                     /locus_tag="PKI68_01355"
     CDS             27881..28771
                     /locus_tag="PKI68_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HNX52371.1"
                     /translation="MKKLKAGLVQQSCTADREANIEKSIAAIRRCAKQGAKLVALQEL
                     HAGLYFCQTEDTHFFDLAEPIPGPSTKTFGALAKELGIVIVTSLFEKRAAGLYHNTAV
                     VLDSDGSIAGKYRKMHIPDDPGYYEKFYFTPGDLGFTPIKTSVGMLGVLVCWDQWYPE
                     AARLMAMAGADILIYPTAIGWDPNDTEGEKARQREAWITIQRSHAVANGIPVLSINRT
                     GFEPSPCGQGGAQFWGSSFVAGCQGELLAQAGTDGETELIVELDGKRSESVRRIWPFL
                     RDRRIDAYSGLTARFLDQES"
     gene            28768..29787
                     /gene="rsgA"
                     /locus_tag="PKI68_01360"
     CDS             28768..29787
                     /gene="rsgA"
                     /locus_tag="PKI68_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNX52372.1"
                     /translation="MTLEEFGFTPWFQQQVDAAASKDCKPARITAIHKGWCDISDGEE
                     TRPAKTTGKLRRAARETKDYLTVGDWVLATNFDAGDFAMIHSVLKRQNTLRRKMAGKE
                     VSHQLIAANIDTAFVVHTLDKGFNLPKLERYLSIVNESGIEPVVLLTKKDMLSAGELK
                     ERLAEVEARLPNVPVYAFSNTTGSGLKKVQKLFEPGKTYCLLGASGVGKTSLINSLMG
                     EEDLYFTLPVRECDGKGIHTTTWRELIELDNGALVIDTPGMRELGHLDTGAGIKDTFE
                     ELTALEGQCRFRDCAHTKTKGCAIEAAVEAGTIPRARYENFIRMRQETAANERALRQQ
                     TWKNK"
     gene            complement(29832..30143)
                     /locus_tag="PKI68_01365"
     CDS             complement(29832..30143)
                     /locus_tag="PKI68_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52373.1"
                     /translation="MKKCPFCAEEIQDDAVLCRFCGQFLVQKTRVPWYLRPGWLVTAV
                     LCVGPFAIPLFWLHPNFSLRKKIVWTAVVVILTWATWILMQKSVQSISDYYGLLDQLM
                     K"
     gene            complement(30153..30869)
                     /locus_tag="PKI68_01370"
     CDS             complement(30153..30869)
                     /locus_tag="PKI68_01370"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNX52374.1"
                     /translation="MSAPHRHRPKGLEIIYEDRDLLVINKDAGLLTMSTPKDELQTAQ
                     HLLTDYLRKGAARSRLRAYTVHRLDRETSGILIFAKTEAVQQKLKGEWPTTEKEYLAV
                     VHGHFREKTGKISSYLAENEAQFVYSTSQTKGRWSETEYTVVRETKEFSELKINLLTG
                     RKNQIRVHMAEAGHSVVGDTKYGLKGTKFERMALHARSISFNHPHSGKRMFFEAPAPK
                     FFNKLMHDSHAPLVNGTGKA"
     gene            complement(30953..31420)
                     /locus_tag="PKI68_01375"
     CDS             complement(30953..31420)
                     /locus_tag="PKI68_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52375.1"
                     /translation="MNKPALLRAIITELENDLRRQQAANEDASAGATHGEARAETKWD
                     TCGLESSYLARGHALQFKALVADLETLRTFMIPVFSGKPIGVGALVDVELDKEKMTFF
                     LLPCAGGTEVRVDGVDVTAVTPESPVGAALLDKKTGAKFSFRAGSSGKVLSVS"
     gene            complement(31540..31953)
                     /locus_tag="PKI68_01380"
     CDS             complement(31540..31953)
                     /locus_tag="PKI68_01380"
                     /inference="COORDINATES: protein motif:HMM:NF013653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNX52376.1"
                     /translation="MSKELEEGLKLELDWNKLEKAVAGTKGIIPVAVQHADTKEVILV
                     AYINQLAFEESLKRKMLVLWSSSRQELWIKGLTSGETFELLEARVNCEQNSLLFIVRP
                     NRGGICHTKNGAGVPRNCYYRKIDLNTLALENLDP"
BASE COUNT         7088 a         9479 c         8658 g         6730 t
ORIGIN      
        1 gttgaaacgc cgtactcttt cgcgagatca gcgagccgtt ccggacgaac gtcggtcatg
       61 ataatttttt ccggcgcaca aaaaccgcct tcgaccatac cgctgacaat ggcttccgcc
      121 atatttcccg caccgacaaa aacgactttt gattccataa tactcatgac cgtcctccga
      181 agatatctga cccgacgcga atatatgtcg ccccctcttc gatcgccacc tcgaaatcgt
      241 gcgacatgcc catggaaagt tccggcagcg gcgtaccggt ttcctgctga agccggtcgc
      301 ggagttcccg aaggtttgaa aaatggatgc gtgctttttc cggatcgggt gtaaacggcg
      361 gaatcgtcat cagcccgtga acttcgacct tgccgcattg gttcgcggct ttaatcagcg
      421 cggccgtttc ttccggagcg cagccgtctt tggatccttc gccggaaaca ttaacctgaa
      481 tgagaaccgg aagcggcacc gccgcgtatt tatccagctc gcgcagcagg tgttcggaat
      541 caaccgaatg aatcatccgg aaaattcccg acgccgcgat gcgcgctttg ttgctttgca
      601 gatggccgat gaggtgccat tgcagatggc cgggacagag cggaattttg gcctgcgctt
      661 cctgtacacg gttttcgccg aacagaatct gaccggcatc ggcggcttcc agcaccgcat
      721 cagccggttt ggttttggaa acagcaacca gctttatttc atcacgccgc cggcccgctt
      781 tttcgcaggc ggcggcgatg cgggtttcaa ccgcttcgag gttttcgcgg atactcatac
      841 ttttgcgatc agtttgatga cgagcatttc gaggattagt tcctgaggaa cgccgctgga
      901 taccagctgt tcgtgggttt cgagcaggcg cttctggcag cggcgcagtt cttcggcgct
      961 gtaatttttc gcctgctggg cgagtttgga cgtgcggaac cagtgcgtct cacgcggatc
     1021 ggacggcagc tgggaaaaca ttttttccat ttcctgatcg ctgctccact gcaccgcatt
     1081 cgcaccgtag ccgccggaaa gccgcaacca gccctggtca atgtacgcac ggtactgcat
     1141 gagattctgg tagaggcttt cgatggcaaa tatgatgccg acgggtgctt cgccctgaaa
     1201 aattagctga cgcaggattt taagcgcttc gtccagcctc cgttcgccga gcgcatcggt
     1261 gaagtcccac gctacggctt cggcggatga cgaagtgatg gccttcacat cctcgacggt
     1321 gatttctttg cggtcgccga tgtagagcgt cagtttctca acttccatga cgagctggcg
     1381 ggtgtcggta ccgacttttt cgagaaacag atcgagcgcg cccgcgccga tgcggcacgg
     1441 cgctttggca aacagagcct gcgcccgctc gcgaatgacc ggtttctttt tatagtcctg
     1501 ttcgggcagg tcataggctt cgactgcgcc ggccgcttca cagattttat agaaggcgga
     1561 gcggcggtcc acgccgggcg ccgagatgat cagatgctgg ctgtccggca gccctttctt
     1621 aatgtcgtcg gccagtttgc cgagaaggcc tttcactgtt tcgtttttgc tgataacggc
     1681 gtctttaaaa atggacacgt cacggagcca gaccgttttc tgcccgccga acaggccgac
     1741 ggtccgcagg gccccgatac aggttttcag cgccgttacc gcttcgtcga tcgtttttac
     1801 atcgccgtcg acggtttcga ggctgagggt ctgttcgttt tccggacaga gcgcgttaac
     1861 ggtcttgcgg gcgttgtgac tcaccagata ttcgtcggtt ccatgtatca gttttacggc
     1921 cacgggttga attccttatt tgcgttgcag gcgcatctta atcataatcc ccgtccatga
     1981 gcaacacgca gaaggatgga atgaaccggc cgctgtgggc accatggcgc gtcgaatata
     2041 ttcacagtga aaagaacggc gagtgctttc tctgccggat gttccgggaa aatactgacc
     2101 gcaaaaatct tttactcgtc cgcggcaaaa cctgcgccgt tatgctgaac cgctatcctt
     2161 acaacagcgg gcatctgatg gttgctccgt accgccacat cgccgagcct gccggtttga
     2221 cgccggaaga aaatctggaa atggccgccc tcaccgcccg cgccgtggac gtgctgcgga
     2281 cggtgatgtc gccgcacggc tttaacatcg gcatcaatct cggcgaagcc gccggcgccg
     2341 gcctgaaaga tcatctgcac cagcatattg ttccgcgctg ggtcggcgac accaatttca
     2401 tgccggttct gggcggaccg cgtgtaatcc ctgaagcgct cgaaatcact tatgatctgc
     2461 tcctgaaatc cttcaaccgt taaccggaga gtcctatgcc gaaaaatata cctgtccttt
     2521 gcgtagaagc tgaaacactg gcggaagcgt acgaaaaagc gctgaccgct ctttacacgc
     2581 gcggaacccg ttttaaaacg cagtacgaca aaccgggcga cccgctcagt ctcgactgca
     2641 ccataaacat caccgtcctt gatccgatga aggatccgat gattcacaaa gcgtttccgg
     2701 gcggcatcgc cgacctgcgc gaatatgtta tggaactcaa gggcttcaaa gatcactggg
     2761 tcaaaaacat gaacgaccct gccgacaccc gttgggaata tacctaccac gggcgccttg
     2821 ccgactacgg tgtctggaag gaactgcaga acggcaaaag cgccgaagcc ggcccgttta
     2881 agatccggca gctcgatggt attatcgaaa aactctcgaa acagccgttc acccggcagg
     2941 cgcaggctat cacctggatg ccgaatctcg accttgaatg ctacgatccg ccctgccttc
     3001 agtccatctg gtaccggata ctcgaagacg aagacggcgt ctggtggctc aacaccaaca
     3061 tccggtttcg cagcaacgat gcatggggcg ccaacttcat gaacatgttc ggcttcaccc
     3121 tcttcagcaa agaagtcatc gccgacgccg tcgcggcaaa aaccggaaag accgtcaaac
     3181 tgggacggct gaactggcag gccgactcct tccacatcta cggtaaagac atacagtccg
     3241 ccaaagaacg gctctttgac cggctgggca caaccgattt cgaggatcgc gtctacgaat
     3301 ttcacgacga aatgatccgc gaaatgtacg atgaggccga agccggggta ctcgccaaaa
     3361 ttaaaaccta tgacgccgcg catgcgcggc cttaacaacg aaggagactg accatgtcga
     3421 aatcacagga tgcaaaaaaa gaaaccaaga agaaaccggc caaaacccaa aaagagaaaa
     3481 agcaggccaa actcgaaaaa aagaaaagcc gatagctcca gcgacggtct ggaacgaacg
     3541 cccggttaag cactcgcgga cttttggtcg cgggtgcttt actgtttcgc cccctgattc
     3601 ctgattattt agggtggatt cgccggaaaa tttctctaca ttctctcccc tcttctatcg
     3661 tttgaaagga cagttttatg gccggcgaat acgtatacaa tctgcagaac ctgaccaaac
     3721 agcacaataa aaagaccgtg ctggaagatg tcaccctcgc ctttttcttc ggtgccaaaa
     3781 tcggcgtgat cggcggcaac ggctccggta aatcgtcgct cctgcgcatc atggccggcg
     3841 tggacaccga atacatgggc caggtgcaga tcgccaaacg ggcgcgcatc ggttacctcc
     3901 cgcaggaacc gcagctcgac aactccaaaa ccgtcatggg caacgtgatg gaaggcgtcg
     3961 cggcctcaca ggccattctc gaccgctacg atgaaatctg ggaactgctg gccggcgatg
     4021 tttccgacga agaatccgaa aaactgaacg aggaagtggc cctgctgcag gacaagatcg
     4081 acacgcagga tctctggaat ctggataacc aggtggaaat ggcgatggac tcgctccgtc
     4141 tgccgcccgg cgacagctcc gtcgaaaaac tctccggcgg tgaaaaacgc cgcgtcgccc
     4201 tctgccgcct gctgatttcc aatcccgacg tcctgctgct cgacgaaccg accaaccacc
     4261 tcgacgcgga atccgtcgcg tggctcgaag aatacctgaa acgctacacc ggcacgctgg
     4321 tcgtcatcac ccatgaccgc tacttcctca acaacgtcac cgaatggatt ctcgaactcg
     4381 atgcgggccg ggcgttcccc tataaaggca actacgaagc gtggctctcg cagaaacagg
     4441 cgatgctcga tgcccagagc aaacaggccg cctcgcgcac caaactgctg gcccgcgaac
     4501 tcgaatggat ccgcaccaat ccctccggcc gccgcgccaa gagccgcgcc cgcatccaga
     4561 actatgaaca gctcgccaaa caggaaatcg acacgcgcga agacaacgtc gaaatccaga
     4621 ttccctccgg cgagcggctc ggcaacctcg tcgtgcgcgg cgaaaacctc accaagtgct
     4681 acggcgaccg cgtcatcttt gaaaacgtca gcttcgacct gccgcccggc ggcattgtcg
     4741 gcatcatcgg cgcgaacggc gccgggaaaa ccacgctgtt ccgtatgatc accggcgcgg
     4801 aagagccgac cggcggcaaa ctcacggtcg gcccgacggt caacctttcc tacgtcgacc
     4861 agtcgcgcga cgcgctcaat ccggacagca cggtgtatga ggaaatctgc ggcggcaacg
     4921 agtggatcga tctcggcggc aaacggatga acggtcgcgc ctactgcggc aagttcaact
     4981 ttaaaggcgc cgaacagcag aaaaaagtcg gcgtgctctc cggcggggag cgcaaccgcg
     5041 ttcacatggc caagctgctg cggcgcgccg gcaacctgct gctgctcgac gaaccgacca
     5101 acgacctcga cgtgaccacc ctgcgcgcgc tcgaagaagc gctgctcagc ttcggcggct
     5161 gcgccgtcgt cgtcagccat gaccgctggt tcctcgaccg catcgccaca cacattctgg
     5221 cgttcgaagg cgacagcaag gtcacctggt tcgaaggcaa ctacgaagcc taccacgaac
     5281 agcgccgccg cctgctcggc gacgaagccg acaaacctca ccgcatcaag ttccatccgg
     5341 tcagccataa gtgaccggaa tcatcatgga aacccttgtt acacgtacac tggaactgac
     5401 cgttgacggc cgcgaccgta aatgtcccgt gtacgttccc ggcgcactga atgccgcgca
     5461 gccggttccg ctcctgctgg tttttcacgg cgcggaagga accggcgctt ccatgattaa
     5521 aaaattccgc gcgctcgccg accgcaacgg gtttctgatc gccgcgcccg acggaatcgg
     5581 cggacggtgg aacgccctgc agccggatga aatcgcagct accggtaacg ctgacgaacc
     5641 tgcgtttgcc gccgccatcc tgaacgctct ggaaaaagag tattccgttg attccagccg
     5701 catctacgcc agcggatttt ccaatggcgg cgcgctgtgt caggtgctcg ccagccgacc
     5761 cgaatcgcgg ctggccgcca tcgcctgtgt cggcgccaca cttcccgccg ttctgcaaaa
     5821 ccagatcgtt tccgcgccgt ccgtttccgt tcttctgctg accggaagcg acgatgagtt
     5881 tttcggcgcc ggaggctcta aactcgggcc gttcctgtcg gcagataaaa ccgcccggct
     5941 ttgggccgaa cataacaaat gctcgctaga gcactttacg attcaaatgc cgcaacccac
     6001 cccggcctct ggccacccct ccgaaggagg ggaactttta aaatcccctc cttcggaggg
     6061 gtgccggaac ggcggggtgg gtttccccgc ctttcacaca acggcacttc aaccgttaaa
     6121 ttctctaact gctcccgcgc gctggatcaa tgaaaaaacc ggtgttgaag tccaatcccg
     6181 ctggatcgaa ggcggcaagc attcatggag actcagtgct gatctggata ccagcctgac
     6241 gatctggcag tttctgagcg cccattcccg atacactcaa aattcctgat tccattggcg
     6301 tgcattagcg ttcattcgcc tgtctcgcca tagcttattg ctaaagcggc ggcggacggt
     6361 tagattcccg cgcatggcaa aactggaaaa taccttttcg tggtcgttca gcgcggcggc
     6421 cgatttcgat gaatgccggc ggcggcgtta ctggagcaag tacggcatgt ggggcggctg
     6481 ggagaaaacc gccgcgccgg aaacaaaaaa ggcctaccag ctcaataaga tggataaccg
     6541 ctgggggctg atgggtcagg ccgctgaaaa ctccgtgatg cggattctgc gccagcatca
     6601 ggcgggcaac ccgatcaccg ccgacgaggc gtttgaaact gtcgcccgcc cgtttctgcg
     6661 ccagaagtgg acggaatcaa aacgcggcga atggaaaaac gatccgaaaa agttctgctg
     6721 cctgcgcgaa cactattacg gcacgatggg cgacgaaaag gccgccgccg aacagatgag
     6781 cggccagatt aaaaactgca tcgctaattt tatcgaaaag gttcttccga gaatcggagc
     6841 cgttacccgc gaacaggaac tgccggtgaa aacgccggat cagggcggcg acccggaaaa
     6901 tatggaattc aagggcgtga agatgtatgt cattccagac tacgcctacc gcatcggcgg
     6961 gcagttccac attcacgact ggaaagccgg caaggtcaaa gaatcgcacc gcgaacagct
     7021 gggactttac gcactgtggg cgcgcgaaaa attccgcgcc aaaccggaag atacgttcct
     7081 gtacgtcgag tatctcaacg aaggagtggt ggcgccgttc cagcttgtac ccgaagattt
     7141 tgtacggctc gaagcgcgcg tcgaggattc cgtggctgaa atgacggagt acctggccgg
     7201 tttcgaccgt aagcgcaacg agccgctgcc cagagaagaa tgggaactgg ctgccgatct
     7261 cacatcctgc aaaaactgca acttctacga actctgcaaa cccgaattgg gagagtaact
     7321 ccgttacccg ttcgttacgg tcggcatcag ccggatgcgg gcgggaataa caaccggaag
     7381 gtctttccct tcaacccagt aggcttttcc ggtgatctca atcttcatgc cggtaaaggt
     7441 cagcatctga ggtttgttcc cgtaaacgtg acagacgatg atgtcggtga aatgatcgtc
     7501 gaccagtttg taaagcatgt tgtcggacgg ttgcagtatg ccgtaatagg ttcctgtgac
     7561 gccctgcggt ttggccggat cgattttgag cttattcggc atattcgtcg ccgaagatgc
     7621 tgaagccgcg atcaggacat ccgcaacggt cggttccgag aacgcctgca caaccgtctg
     7681 cgtttcttga accgcggccg gaaccgcatc cggccctgca aacgcattgc cgggaacggc
     7741 ggtatatttg cggctgaccc agatgaccgt gttggaggtt ggcgcaattt tcagccactg
     7801 tgcaatctta ctgcgaaccg tcagaatatc ccctttgtgt gccgtgccta cggtaccgtg
     7861 gctcagactc ggcccggaac ggatgttcag cagttccggc acaacggtgt tgctgatgac
     7921 aaattcgctg ttcacccaaa gatcgactgt gtcagggggc tgaacgccga cccagtccgg
     7981 attactgttg tcccgaagcg tcagttcatc gccgagcatt gcgcggccca gcagtacagc
     8041 gttcgtttcc ggcgcagcgc gcaggctcac ccggtcgccg gtcattttta cttttaccgt
     8101 ttcctgaacc gtgttcgtcg cggattcctg tgcgtaaacg gcaaacgtta aagcaaacag
     8161 tgaaaacaga atttttttca tagcggctcc tctttcagtc cgaacaaccg ggttgcattc
     8221 tgagcggttg ttttcgccag cgactctact gtacagccgc gcagttccgc aagccgtttg
     8281 gccgtatcaa ccgcaaaggc cggttcgcag cgttcgccgc ggtgcggaac cggcgcgaga
     8341 tacggcgcgt cggtttcaat cagcaggcgg tcgtccggca caaactttgc cacctcgcgc
     8401 agcgggtcgg cattcgcaaa ggtgacgatg ccgctgaaac tgatatagaa accaaggtcg
     8461 agcagtgcgg ccgccatttt ccggtcgcgg gtgaaacagt gaatgacacc ggcgcgggcg
     8521 gcatcgccct tccagtgctt ggcgtaatcg gtgaggatcg aaagcgtgtc atcgtccgcg
     8581 tcgcgcgtat gaacaattac cggcttacgg gtatgaatgg ctgtttccag gcattgaaaa
     8641 aacagccgct gctgttcttt ggcttttccc ggttcataaa aatagtcgag tccggtttcg
     8701 ccgatggcca cggttgaagg ccgtgcggcc agttcccgca gtgcgctgat gtcacagatc
     8761 gaatccgcca tgtggcgatc ataaccggcg gcggcataaa tccgttccgc aaacgcctcg
     8821 gccagcatct gcgccagttc attggcttcc ggagaaccgc cgacggcgat cattttcgaa
     8881 acggctgctg tttccgcgcg ctccagcaac ccttccagcg tgccgtcttt tacaaaatcg
     8941 tcaaaatgaa catgtgtatc gaaaaacatg cggacagagt accgggaacg caaaaagata
     9001 taaacaccgg agtatcggaa taatgtaaat ccacccccga tttaggagtt taggatatta
     9061 ccaaccgact gaattccgct aggatatacg acgtaaagga ggacagaatg aacgcattaa
     9121 acagccagac ctgtgaagcc tgtcaggcga atgctccgca acttacagca gaagaaatta
     9181 acgaactcct caaacaactc cagaactgga agcttatcga agctgacgga gaaaaacggc
     9241 tggaacggaa gttcaagttc gataatttcg ccaaagccat cgctttcacc gaccgtgtcg
     9301 gcgcactggc tgaagagcag aaccaccacc ctgcactgtt gaccgaatgg ggtaaagtca
     9361 cagtcacatg gtggacacat aaaatccgcg gcctgcataa aaacgacttc attatggcgg
     9421 ccaaaaccga tgaacttttc taacgaaagg cggcatacca tgatcgacat catcaaagcg
     9481 gaccagcgcc atttttctga ctttcactgg ctgaaaacct attggctctt ttcattttcg
     9541 gattatttcg accctcataa tattcagttc ggggcgttgc gcgtttttaa tgacgatgtc
     9601 gttgagcccg gcaaagggtt cccgacacac cctcacaatg aaatggaaat catcaccatc
     9661 gtactcgacg gtgaaatgac ccacgaagac agcatgggta ataagaccgt gatccgatgc
     9721 ggcgatgtcc agcgcatgtc ggcaggaacc ggtttaacgc actcggaatt caatttggcg
     9781 gaagagccgg tgcattttta ccagatctgg attttccccg atcaggcgaa actcacgccg
     9841 tcttacgacc aacgcaactt taaaccggaa cagtggaaaa actgcttact gccagtggca
     9901 tcaggccgga atattcccgg aacagtgacg ttctttactg atgcgacgat ttaccgttcc
     9961 gctctggatg ccgggaaaaa agtgtccttt gagaaaactg ccggacggcg gatctttgtt
    10021 tatgtaacgg cagggcagct cgagatcaac ggcacacacc ttgatgcgaa tgatcaagcc
    10081 cgtatcgacg aagatggttc actcaccatc aaagcgcata aagccgcgga gttcattctg
    10141 atcgatgtgc cctcctgcaa aggctggggc tacagcgaaa aaatactgca cggcgcatcg
    10201 cggcggtaaa attacagatc cagaaaattc tgcaaaagct gtttgccgtg ctcggtcaaa
    10261 atggattccg gatgaaactg gacgccgtgc accggatatt cggtgtgcat caatcccatg
    10321 atttcacctt cggcggtttc cgcggtaatt ttcaggcagg acggcaggct ttcgcgctca
    10381 accagcagtg agtggtagcg cgtggcattg aacgggctcg gcagtccctt aaagacgctt
    10441 tcgcctttgt gcagaattgg cgaggttttg ccgtgcatta accttgcagc acggacgacg
    10501 ttcccgccga atacgtcacc gacagcctga tggccgagac agacaccgag gagcggaacg
    10561 cgcggaccga attcacggat aatatcgcag gagacgcccg cttctttcgg tgaacacggc
    10621 ccggggctca ccagtacttt ttccggtctg agcgcaacgg cttctgcaac cgtgatttta
    10681 tcattccgga aaattttcat ttccgcgccc agttcgccga gatactgcac gaggttgtag
    10741 gtgaacgaat cgtagttatc gatgataaga atcataaggn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnng ctgcggcata atgcttggcc agcgaaagcg ctttcagaac
    10921 agcgccggcc ttgttacggg tttcctgata ttctgtttcc ggcaccgagt cggcgacaat
    10981 gccgccgcca gcctgcacat aggctttgtt tttatcgagg acaaccgtgc ggatggtgat
    11041 gcaggagtcg aggttgccgt cgaaactgaa atatccgaca gctccggcat acggtccgcg
    11101 tttggttttt tccagctccg caataatttc catggcgcgg attttcggcg caccgctgac
    11161 ggtcccggca ggaaatgtcg ctctcatcac atcgtatgca tcgtattccg gagacagcgt
    11221 tccggtaacg tcggaaacga tgtgcatgac gtggctgtag cgctcaatga ccatctgctc
    11281 cggcacacgg acacttgaga attcgcagac acggccgacg tcattgcggc cgagatcgac
    11341 cagcatgaca tgctcagcca gttctttcgg gtcggccagc agttcctttt ccagcgccag
    11401 atcttcggct tcggtttttc cgcgcggacg cgttccggca atcgggcgga gctcgacctg
    11461 ccggtcttcg cagcggacat gaacttccgg cgaagagccg accagcgcgc tttcgccgag
    11521 gtcgagacag aacatgtagg gcgacggatt aatcgcgcgc agtgcgcggt aaacgtccag
    11581 tgaatcagcg gcattttcga cttcgaaccg ttgagaaagg acggtctgga tgatgtcacc
    11641 cgcacggata tattccttgg ctttttcaac catttcccgg tattccggca ccgtcatgtt
    11701 ggacaccggc accacctgtt ccacgtcaag atgcgcatcg atcagcacgc ggctcaccgg
    11761 ttgcagcagg gcctggcaca gttcgtcgat ttccgccagc gcttcgtcat acgctttatc
    11821 cgggtcgccg gaaacgtacg cattggaaac aaccttcatg gtgtgtttta cgcggtcaaa
    11881 aatgatgatg gagttcgtga tcatcatcac catatccggg ttcccgataa tgtcggcttt
    11941 aatcaccgga acgcgcggtt caaagacgga aatcatgtcg tagccgagga atccgaccgc
    12001 tccgccggta aaccgcggca gggccggttc gcggaccggt tgataacggc tcatgcaggc
    12061 tttgagcaca ttgagcggat ccgtattctt aatgatcgtt gtcgtgcctt cttcggttat
    12121 ttcagtgaca gctccccggg cgcggacgat ggcgcgcgga ttgccgccga tgaaggagta
    12181 gcggccgatg tgctccccgc cttcgacgct ctccagcatc caggtgtgtg acgcgtgatc
    12241 tttttcgcgc aggaatttgc gcacccgttc ataggcggaa accggagtct cctgatcggc
    12301 cagaatttcc ttccagaccg gaatcagatt cccctgcatc gccttcttta aaaattcagc
    12361 tctgtttggt tgcacactca tggtttgccc tgtatttgaa gcgcttttgt acccgcaaca
    12421 gccctctggc gcaagtactg cgtttccaat gtttgaaaat tatcttccag aaaaccgtca
    12481 cagaagagaa ccctgatctg ctataaactt ccggtatggt ttctgcctca accaaaagcg
    12541 ggttttcgat caagcagttc ctgagttttc tcggacccgg ctttctcgtg accgtcgggt
    12601 ttattgatcc cggcaactgg gcgaccaata tccagggcgg ctcgcaattc ggctaccggc
    12661 ttctgtgggt aatcacactc agcaccctga tgctgatcgt catccagcac atggctgcca
    12721 agctgggaat cgccaccggc aaatcgctcg ctgcaaacat ccgtgaacgc tttccggctc
    12781 cggtatcggc atttctgggc gtgaccattg tcctcgcctg cgtcgccacc gatgtcgcgg
    12841 aactgatcgg cggagcgatc ggcttcaatc tgctgttcgg cctgccgttg tgggccggcg
    12901 cgctgataac cgtttttctg gaagtcttcc tgctcatcag ccagcgctac caccgtctcg
    12961 aaaccattat tgtcgggttc ctcggaatta tcgcctgctg ctatgtgatt gaaattctga
    13021 ttgtcaaacc cgactgggcg gtgctggctc ccgcccttgt cattcctcat gtggaccgga
    13081 acagtattta tgtcgctatg gcgattctcg gcgcggtcgt catgccgcac aacgttttcc
    13141 tgcactccaa cgtcatccac agccgcaaat ggggaatctc cgaagccgaa aagatgacgc
    13201 ttctccggta cgaaaaatac gacacgctgg ccgccatgct gctcggctgg gttgtaaact
    13261 ccgccatgat catcgttgcc gccgcggttt ttgcgcgcca ccaggtcgcc gtcaccggca
    13321 ttgaacaggc ctcagaaaca ttgcgtccgc tggccggacc gctggccggc ctgctcttcg
    13381 ccgtggcgct tgttttcgcc ggcgtcggct cttcgatcac ctcttcaatg gcggaagcca
    13441 atgtaatcac cgggttcctg ggcaaaccgg aagacccgcg caccctgctc taccgcgttt
    13501 ctgttttcat caccgccatc ccgtcattca tcatcatcct gctcggcatg gataccttta
    13561 aaatcctgat tttcagtcag gtggtactca gcattcagct gccgttcacg ctgattccgc
    13621 tgctcattct ctgccgcagc cgcaaggtca tgggagcctt ccggagcggt cggagagaat
    13681 tcatcactgc aaccgtcatt gcagccgtta tcatagcgct taacatctac ctgctctact
    13741 caacgctcgc gggaggaacc tgacatgcaa gtgtacaaaa acatcctcgt tacgctcgac
    13801 tgttcgccgg tggatgatgc catcatcaac catgttgtgc tgctggccgt tcagaaccgg
    13861 gccaaagtac atctgctgca cgtcgtgcat gcccacacac tggaccagcg ccgcgcactg
    13921 cgcgcagaag ccggcccctg tctcgaaaaa cacgccggaa ctctgcgcga acagggggtt
    13981 gacgctcata agattatccg cgacggcgaa ccggatgaac agatcctcaa ggagatagaa
    14041 accggcagct atgatctcgt cgccatggcc acgcacggcc ataaattttt cggtgacatc
    14101 cttttcggca gcgtctccaa aagcctcaaa cacaagatcg gcatcccgct cctgctgctc
    14161 aaaccataat gccgtccgac accgcctgtg cggcggcagc aaactgatcc agatcatgct
    14221 ctgtatgagc cgcgctgatg aatgccgctt caaactgcgc gggcggcaga tagaaaccgc
    14281 gttccagcat gctgcggaaa aacgcggcgt acgctgatgt gtcgcaggat ttcgcatcgt
    14341 ccagattccg gaccggatcg cgccggaaaa acggtgtaaa aactccgcct gactgcgcaa
    14401 catgaaattc gcctgcaatt tgattcagct tagccgacat ctttgcgccc gcttccgcga
    14461 gacgcggata aggattttct ttaatcagca gttccaccgt tttcatcccg gcggcaaccg
    14521 caaccggatt accactgagc gttcccgcct gatagacatt gccgaccggc gagaggcgct
    14581 gcatcacatc gcgccgtccg ccgaacgccg ccagcggcat accgccgccg ataatcttac
    14641 cgaggcaggt cagatcgggc gcaatacctt tcagcgttcc gtagctggtc ggcccgaacc
    14701 ggaaaccggt aatcacctca tcgaaaatca gaagcgcgcc gcaacgatcg gcttccgcgc
    14761 gcaggtgttc cagaaaaccg tcacgcggca gaaccaatcc catatttccg gccaccggct
    14821 ccacaatgat cgccgccagc tcatcgccgc attcgttcac aatccgcgaa accgcttcct
    14881 tgtcgttgta attcggcaga aaaacatcgc cttcatttac ataaactccg gcagaggatg
    14941 aaatgccgcc ggtcagcagg ccgctgcctg cgccgaccag caggccgtcg gaatggccgt
    15001 gatagccgcc ggcgaattta atgatccgcc gccggccggt aaacccgcgc gctaaacgca
    15061 cggccgtcat cacggcctcc gtgccggaat tgaccagccg ggtctgctcc atcgaaggca
    15121 cctgcgcgca aagcagttcg gcaaactcca cctcgcgcgg cgtgttgatg ccgaaggtca
    15181 tcccgtcgcg ggcggcggca caagccgcat cgaccacctc cggacgggcg tgcccaagaa
    15241 tcagcggtcc ccacgacccg cagaaatcaa tcagctcgcg gccttcagcg gtggtaatcc
    15301 tcgcgccccg gcctttcgtc acataaaccg ggccgccgcc cacggcgtta aacgcccgga
    15361 cagggctgtt cacaccgccg ggaattacct tcagagcccg cttgaaccat tcgtttgatt
    15421 tcacggtttc agtccttcaa cagttcgttg tagcggctgg cccagtagga gatgaaaatg
    15481 ccggtcccgg cacgctgcat cgccgccata ttttcgcgga ccatcgcgcg cagatcgccc
    15541 cagccgcgtt ccgcctgggc aatcagcatg gaatattcgc cgctgacgtt atacgcggcg
    15601 accggcaggt cagttgtctc gcgcagttga tgaataacgt cgagatacat catcgccggc
    15661 ttaaccatga ggatatccgc gccttcatct tcatcaaact ccgattcgcg cagcgccgtg
    15721 cgcaaatcgc tgtacgaagc ctgatagctc tgccggtcgc ctttaccggg agccgactgc
    15781 tccgcttcgc ggaacgggcc gtacatcgcc gaggcaaact tgctggaata actcatcagc
    15841 agcgtatcgg tcagttcctc ttcgtcgagc gcttcgcgga ttgccagaac ctgcccgtcc
    15901 atcatggcgc tcggcgcgac gcagtccgcg ccggcctgcg catgcgaaac cgccaccttc
    15961 tgcagcagcg tcagcgcggc atcgttatcc acctctccgg cggcattcag cgggccgcaa
    16021 tgtccgtggt cggtataggc gcaaagacag acatccgtga aaacggtcag ctgcgggaag
    16081 gctttcttga tggcgggaat cgccttttgt accgcgcctt tttcgttaaa ggcctccgag
    16141 ccgttcgcat ctttcttcgc cggatcggtc agcccgaaaa ggagtacgcc gccgactccg
    16201 ctttccgcca ccggtttcag ccgtttaaga agcacatcaa cgctcatccg cgactggccg
    16261 ggcatcgcgc tgatcgcttc atcaacaccc ttcccttcgc ggataaaaac cggccagatg
    16321 aatttttccg gaccgggcag cggctgtcca agcatccggc gcagtccttc ggtgcggcgt
    16381 aaacgtctca gtctgatttc aggaaacatg ggttattctc cggttaattt cataaacagc
    16441 caaagcgcgc agactctctt ccacggtcgc catttcgccg atgacgccgg cgttcaaacc
    16501 aaaggatttc aacgcagcct gcgtcgggcc gccgattgcg agaatgattt tatttttcaa
    16561 aacggctgcg ccccactgct caataaagga ttccaccgcc gatgcactgg caaaatagac
    16621 gctgtcaaat tcaggcattt ccggatagtg aaccggcttg ttcgtgtaaa gtacgcaatc
    16681 cgtaacctcc gcgccggctg cgcgcagtcc gtcagccagt tccgttccgg cattctgaga
    16741 acgaagccgc aggattttct gtcccttcac caccggcacg gcgtcggcga gcagacctgc
    16801 accgctgaag tcgctttcgg gcatcaggtc gcaaccgagc ccgatccggc ggagcgccca
    16861 tagggaaccg gagccgacgc tcatgatttt cggaattcgc cgcagatcaa ttttttcttt
    16921 gagaagcagt tcatgaaaac agcggacagc agacggcgat gtcagcgcga tccagtcgta
    16981 tgaagcaaga tgacgaaccg catccagccc gtccgttgaa accttcagcc ggatcagcgg
    17041 gcgcgccacc gggcgtccgc cggaatcgtt aacggcctgc accgcttttt ccatcagggc
    17101 ttcgctgcaa gtgagcagga cgcgctgtcc gccgagcgcg ccgagcgagc ggtcaaaacc
    17161 gtagcgggcg atttcgccga cgataaacag ccccggcagc cggccggtga cttcatcgac
    17221 agcgggcagg ttcccgagcg ttgatctgaa tactgcttct ttttccgagc ccgctccgaa
    17281 gaccagcgca cacggcatag ccggaggaac accgtccgca atcatctcct gcgccagttt
    17341 ttctgccgtg ccggcggcca tgtaaaaaac aacgggaagc tctgcccggg cggcttttgt
    17401 aacggggccg attcctccgc cctgcaagcg cggagtcatg acagaaaatc cgcgggcgac
    17461 gccgcgccgc gttaaaagaa taccggtatg cgcggcggcg gtttgcatgg cgctgatacc
    17521 gggcagaaca cgaccggcca gtcccagcgc ttcgagagcg tccatctctt cggccagccg
    17581 cccgaaaatt cctgcatcgc cgcctttgag gcgaacggtg cgcagacctt tgcgaacgta
    17641 atcgcacagc agccgattga tttcgtcctg tgactgcgaa tgcgcgccga gccgtttgcc
    17701 gacgtaaact ttccgtgccg tttccggcag gtggtgcaaa agcgattcat ccatcagcgc
    17761 gtcataaaga cagacctcgg cggtcctcaa cgcgtgcaga ccgtcaagag tgcaatgtcc
    17821 ggcgcagacg cccgcgccga caaaggttgc gcttttaaca aacaggctgc gaatcgcgca
    17881 gagccgcgga tcgtttttac gaaacgttac cgccagagct ccctgagact ccgacgtttc
    17941 cagctgatcc agcggaatcc attcagtgat acggtcttcg agtcccagcc gctgcagcgc
    18001 gacgccggcc atgattaaca aatcaaattt tcctgcgtcg agctgctgaa tccgctcttc
    18061 gatattgccg cggaccggat actgttttgc gaacgggaac cgtttagccg cataggctgc
    18121 gcgacggtcg ctgctcactc cgatcacttt tggattcagc gatccaacca gcacgtcccg
    18181 gcggtcgccg gggtccggga gccagaacca gtcgatacct tccggaaccg gctcaggcag
    18241 gtctttggcg ctgtgcacag caaagtctat ttttccggcc agcagcgctt cgtcgagcgt
    18301 gcgggtaaag aaatccgccg gactttcgcg aagatcggtt gtgcggtcag catctccggg
    18361 cgcagtgatt tcgacggttt gaaactcatg ctccggcaga aatttccgca gaatctccag
    18421 cacccgggca gtctgccaga gcgccagccg gctattgcgc gttcccgccc tgaaaacttt
    18481 taatgatccg ttcatactga tcctgatgct cttctatgat gccttggcag cggtgataaa
    18541 ttttattcgt ttcggcggtt ccatgccggt accggcattt cagaccgtcc agatcgacga
    18601 ttcggagtcc cggcaccagc ccgtccagtg caggttcgat attgcgcggc ataccgagat
    18661 caatcaagag cacaggtttc tgccggttaa aaaacggagc gtgtccccga tgcagcacat
    18721 ggccgggcgc cccggcggaa ctgatgatca cgtccgtctc ttcaagccgg ttcatgaagt
    18781 cattaaacgt gcagagttca acacggtcct tccactcacc gggaatctcg ggacggttga
    18841 tgtggcagca ccagatgatt tttccggtct tttgaacggc cttgcgaacc agcgcctctc
    18901 cgatcctgcc ggcacccagt accatgacgg tttttgagga gagaccgggg atttgagtct
    18961 gaagatattt cagagcgata tcctcaattt cttcactgca gaatagcggt gccacttcag
    19021 tttgaatgtg cttggacaca tgaagcagcg tggaaaccca ctcctgcatc attctgccgg
    19081 cccagccgcg ccgctgtgct tcggccaaag catctttgat ctgcgcggcg acatggctct
    19141 cacccggtgt ctgcgacagc atgccggcgg aaaccatgca aaggtgcccg taggcttcag
    19201 ccccgtgctt gaggtaaaat ttgtcttccg tgagcttatc gaatccgagg agatgacgca
    19261 ggatgccgtt ggtgctggtt tcccgggatg ctaccgcaat taattccacg cgattgcagg
    19321 tattgagaat catgaattca tggatgcccc acagctgcat gatcatccag ccgatacgtt
    19381 caaagcgcgg ccccgtcagg tgaaacggtt cgcgctcttc gatatccatg tggtgatggt
    19441 cggttccgac aacgacaaga tcggcggttt caaccatcgc aatgtgtttt gccaggttgt
    19501 ccttgatcag cttagcctga cggcaggaat gtccgtctgt ggaaaccgtc agggtaaggt
    19561 ttccgtgacg gctgattgcg ggtgtagtga aatcgctctg agcccaatgt ccgtcgacgc
    19621 agctgcaaag aaccttgtgt ttgcggcagt catccagaac cagccggttg acgtaccggt
    19681 cgttcgtagc ggcaaaaaca actgcaaaac cggccgtatc atctgattta aacggccggt
    19741 tcagatgcac aatgcgctgt tcgctgatcc acgccgcagc ttcttcggaa acctcggggc
    19801 tgaccactgt aactttcgcg ccggcttcaa gcaggtatcc gatcttgcgt gacgcaatcc
    19861 gccccgcgcc gaccaccaga caagggcggt ccgctaaaag caggttgaca ggcaatgcac
    19921 tggttttctg cgactcactc atggttaaag tacttcatac tgtctttttt aaattcacgc
    19981 ccggaggcga acagctcgcc attctgcagg tcgcaggtga aagaaatatc cttatacagc
    20041 gccttggttt cgtcccagct tccggtatgg atcatcgcat aaagatcaaa cggaaaggtt
    20101 tcaaggcggg ggcgctgata acaatgagtg acttcgggcc gtgcggccgt gcggcgccct
    20161 gcggcggcga tgtcaccatc gaccggccag acacacatgg cgttggcttt aaacccggct
    20221 tcgcggtgat aaagaatcgc gccgatacgg cgaagcacgc cctgctcttt ccatatcttc
    20281 agcgtggaaa gaatattctc ttcagtctgc ccgatcattt cagccagcgc cgcgaaagga
    20341 cgttcggtga gcggaatctg ctggtccagc acgcggacca gcgctttttc atcggcggaa
    20401 aatgtgcgga acgtttcgtc ttgcgacgga acagcggacg gatcgagtcc gggaaaaacc
    20461 tcgtcgcgat tacgggtctg aagatcgaaa acgacatcga ttttaaaccg gcgcaacgca
    20521 ggcagctcaa acagtgtgca tggggcgatt tgcgcgcgca gcgcggccag accttgatcg
    20581 aactcatcat ggagcatcgc cagcgtaaac cagagccgcg gatagttccc tctgccgtac
    20641 aacggcagac gctcatagca atgcgtcacg cccgggtgcg cgccgatgat ggcggccttt
    20701 tcctctattt gctccgcaga aacgtccagc gcacaaagaa tgcttttata gcccagccgg
    20761 cgggcgtcga atatgccgcc gaaacggcgc agcagtcccg cttctttcgt acggcgcaac
    20821 agcgccggta cggcgtcttc cggaatatcc agattccgga agggccgcga taccggctca
    20881 attccgcgtt gaagcttcgt gagcactttt ttttcagagg atgaataacc ggtcattcgg
    20941 cctcttcttt catgtagtcg ccggtgcggg cataggcgcg ggccgggcag ccggcgaacg
    21001 gattgagtgt tcgcatattt ttaaacacat ctgaagtatg gcacagggtt tcgaaatcga
    21061 aaccggcggc atgcaggtcg ccgcagggca gatcaagaaa tccgcagggc tggagaatgc
    21121 cggtatgcga aataaaaata aacccgcgtc cgcccatgca acccataaac ggcggggtgt
    21181 gttctgaaga aatgaacttt ttatactgag gggcacaggt cgttttgaca cgaaccggcg
    21241 cactcttcgc ctgagttgca atccattcaa gcgcacgatc acgggctgcg ggactgagag
    21301 cgagatcggt catttgtgcg ccgcgcccgg tcggaaccag aaaaaagaaa tcgattgtcg
    21361 ccgccccgag cgtaaccgcc tgttccagca gggccggcag ttcatcaaca ttgagttttg
    21421 aaacggttgt attgatctgg aacggaactc ctgccgcctt aaggtggttc agtccgttca
    21481 gcgttttttc atacgcttct tcgacaccgc gaaatgcatt gtgtgcctgc gccgtggcgg
    21541 catccaaact gacactgacg gccataacgc cggacgttct gagctccgcc gcaacatccg
    21601 gcgttaaccg atgaccgcaa gtcgccaata ccacgcggca gcctttttgc gcggcatagc
    21661 gcgcgacgtc aaagatatca gcgcgcatta gcggttcgcc tccggtgaga atgatcatcg
    21721 gtttagaaaa tgccgccagc gcatcaacgg ttttcaaaca ttctgcggtg gaaaattcac
    21781 cggcatagtc gcagtcgcgg gccgcaccgc ggcagtggcg gcagttcaat ccgcagcgcc
    21841 gggtgatttc ccatgcaatg atcgaaggat ttctgcctgg cttatgcacc gtgaatctcc
    21901 tcatcggtca gataacaggc cggatccggc gcccacagat cgcctgtaga ggcttcggcc
    21961 cgggcgcgga agttgccgcc gcacagttcg agatactgac aatcagcgca acgtccggtt
    22021 acatgagccg cctttttctt gagtttctgc agaaattcat tttccggatc agtccagatt
    22081 tctccaaaag gggttttgag tacattgccc agctttttac tgcgccagaa ttgatcggga
    22141 tagacgtcgc cgttccagct gatacaactg atgccgacgc cggagccgga agccgctccg
    22201 ccgttgagtt tgagcagatc ataaacgtgc gccgcgtcgg gatggttttc ggcgcgcatt
    22261 ttgcgcagca gccaggggcc gtcactgtga ttgtcgacgg tgagcacttc aacttttttt
    22321 cccttcgcat gaagttctgc agttcgttta ataatcagtt caagcgcggc gcgggttgct
    22381 tcgtgcgacg gcatgccgtc gtcgttattg agatcttctc cgcggccggt gttgacgaga
    22441 tgatagaagc aaatgcgggg aatgttctca gcttccatca gatcaaagac ggccgggata
    22501 tcagcgatat tatctttaac cagggtaaac cgcagtccga ctttcagtcc ggcgtcgcga
    22561 cagttgcgga ttccgcgcag ggcggcctga aacgcgcctt ttttgcagcg gaataaatca
    22621 tgcgtggtct cggtgccgtc aatgctgatg ccgacatagg acagtccgat cttttgtaag
    22681 cggccggcaa gcggccggtc aatcaatgtg ccgttcgtcg agatcactgc gcgcagtcct
    22741 ttcgcacggg catattcagc cagttcgggg acatctttac gaacgagcgg ttcaccgccg
    22801 gaaaaaagca gaaccggaca gccgaaggag gcgatctcat caatcatttt ttttgcctgc
    22861 gccgtggtaa gctcatccgc acaggttgtc gcgtcggaac gcgcgtagca gtgggcgcat
    22921 tcaagattgc aaaactgtgt acaattccag accacaatcg ggcggcgcac ggacgccgga
    22981 cggcgggtca atccgtaacg taattcgtcg gatgattcga cgttatccag atataacttt
    23041 gaaattccga gcatattttc actccgttgc gcctctgcat ttccggagcc cttccgcaac
    23101 cgaagatcat ctcgtgattc aacggatgat cggcctgttt atatcacata gatccgatct
    23161 aaatgccacc catttaacca gtatgcacaa ccggcagccg gcagcccggc ggttaattaa
    23221 tgaaagattg cccccggttt tatggtcatt aaacacggct tactctcatt acggctatga
    23281 ttttctgaaa gcgctgtaat tcggcagaga atatttttcc aaacccggaa atgcccgtta
    23341 tactgcgccg caatgaaacc aatctgtaaa actgaaaacc tgcgcgttga aagcgtcacc
    23401 aaactgatca cgccgggaga actgaaaaaa acgctgccgt taacggaagc atcgaaggaa
    23461 acggtcgtgc gcggacgcga agccgtctgc gccgtactcg agcgccgcga cccgcgcctg
    23521 ctcgtcatca ccggcccctg ctccattcac gacccggccg cagcgctcga ctatgcccgc
    23581 cgtctgaagg ccctcagcga agaggtcaaa gaccgctttc tgattgtcat gcgtgtctat
    23641 tttgaaaaac cgcgcacaac catcggctgg aaagggttca tcaacgaccc gcacctcgac
    23701 gattcctgcg acatggaata cggcctgcgc gaagcacgaa aactgctgct gtccatcacc
    23761 ggactcggtc tgccggtcgc cactgaattc ctcgacccga tcattccgca gtacaccgcc
    23821 gacctcgtca gctgggcggc gatcggcgcg cgcaccattg aatcgcagac ccaccgcgaa
    23881 atgaccagcg gactttccat gccggtcggc ctcaagaatg ccaccaatgg aagcgtccaa
    23941 agcgccgtcg acgccctgct cgcctgccgt tcaccgcaca gctttctcgg aatcgatcag
    24001 gacggacgga gctgtatcgt aaaaacccgc ggcaatccgg acacacacct ggttctgcgc
    24061 ggcggcggcg gaaaaaccaa ttatcacgcg gacgaagttg ccgaagccgc cgccaaactg
    24121 cgcgccgccg gcctgccgga aatgatcatg gtggactgtt cgcatgccaa ctgcgccaag
    24181 gtcgccgtgc gccaggcgga agtctggaaa gatctgctcg aacagcggaa aaagccggac
    24241 tgcccggtca tgggcgccat gctggaaagc ttcattgagg aaggcaatca gccgatccgt
    24301 gccggtctga aatacggttt atcaatcacc gatccgtgca ttgactggaa catgaccgaa
    24361 caactgctgc ggatgtagaa tccgcagcgg agacttcgct tcactgaagc gctgcgaagt
    24421 ctagacgaag gcctgggtga ggagttcctg tatctggcgc cggcgggttt cgatgctttc
    24481 gcacaggcga aactggatct gggcacgctg gaggttctgc tccggcgact tgactttgaa
    24541 gtaaacattg cccgccagat agtcctgaaa gaaacgcagg cccagttcaa acgtgatcag
    24601 acggatggag tcgtacaggt attttttgtc gttttcggtg aggaactcct tggcgtagac
    24661 gatgtatccg cgcacgaagg cttcgcacag gtcgagatcg aaggaaactt tacccagctc
    24721 atcggtttcc tccccctcct gattgcaaag ggatcgcagc gcatcaccga aatcataatg
    24781 gatgagcccg ggcttggcgg tgtcgagatc aatgatcgcc gtgcctttgc cggtatcatc
    24841 gtcaatcatg atgttgttaa ctttcggatc gccgtgaatc agccgcaagg tcagctcgcc
    24901 cgcatccagc gcgttctgca aaacataggc gaacttgcgc cgggcttcga caaaatgctg
    24961 caggttgcga acttccagcg agcgtttgat gagctcctgc gcttccggcg tccggaccac
    25021 tgcatcatat ttctccagat atttcggtgt ttcgtggaat ccgggcagcg tgtcgcgcag
    25081 gctgaaggga ttcatgttcg aaaccagccg gtggaactgg ccgagtaccg ttccaacttc
    25141 aaaggcatgc tctgcgctct gagcggtatc gaaagaagtc gccgaagcga tcagcgtcag
    25201 cgcccgccag taatcgccgt tttcatcttt aaaataatcc tgtccgttgc ggcacgggac
    25261 gatgcgcggc agctgccaga tgcggtcggc ccggtcgctt tccgcctgaa gctgtttgtg
    25321 acagtggctg gtgagaatgc tcagattttc cataatccac tcgggatgat ggaagacccg
    25381 ttggctgatg cactgaatga tgatgcgttc ttcggaaaaa tgggtgcgga aaacggccag
    25441 atacgtatcg ttcacattac cgctgccggt cggacggata ctcgccagct gtccggacac
    25501 tacaaactgt tctgcaattt taaccggatt catttattac accctccgct tcgtcacgcc
    25561 gtcacacagt tgaaaacctt tagcaaaccg caaccatgat accaatcctt ttcggtcgta
    25621 aacccgataa ataccgcaaa ttaacgatcg gccggcggtg actggttcag ctttgcctta
    25681 aaaaacgaaa cccgccgcaa agcctcatca atccggcttt ccggaacgcg tccgctccgg
    25741 accagctccg cgacaatctg cacggttttc ggcaccagat ccggatcgta ggtctcatta
    25801 ttggcgatca gcagaatatc cgcaccggca ttcaggctgt attccagcgc cgcctcaaaa
    25861 ccgaaatgat cggcaatggc tttcatgcca aggtcatcgg tcatcacaac tccatcgtag
    25921 ccgatctgat tccggagcag gccggtgatg atgcgattcg aaagcgtcgc aggcagttcc
    25981 ggatccagat gccgctggaa aatgtgggcc gtcatcacaa cgtcggtttc tttgcagagc
    26041 tgccggtagg gttccagttc ggcgccgctc caggtcgccg tcacgtcggt catatcttta
    26101 tgcgaatcag ccatcgaact gccatgtccg ggaaaatgtt taagggtaca catcacgccc
    26161 tgatcgtgat gagcccggat aaactgaccg gcattccgga tggtctgtgc cggatcggcc
    26221 gaaaaggtcc gttccttttt aacgatgaaa ttatccggat tcacggccag atcgactacc
    26281 ggtgccaggt tcagattaat tccggcggct ttcagcgccg aagccagctg ccgtgcctgc
    26341 cggtatgtaa actccggatc gttgcgctcg ccaagctgct gctgagaaat cgtcggcgga
    26401 aatccataag ccggtttcag ccggttgacc cgtccgcctt cctgatcaat ggcgatcagc
    26461 agccggtttt ttgcaaacga ctgaagcgtt ccggtcagct gcgccagctg ttccggtgaa
    26521 aggatattcc gttcattcgt tttcagctca aggtcagcat caaacaggat tacgccgccc
    26581 agattgtaat cgcggatatc gcggacaacc ggactgtttc catccaccga cataccccgg
    26641 aatccgacca tcagaatccc gccgattttc tgttcaagcg acagctttgc agcaggcggc
    26701 gctgcagtgc aggcgacaag gccgggaaac agcagcaaaa gcagaaactt aattttcatg
    26761 cgccattctt cccacaccgg ccggaatgaa tcaatccctc gcgcaaccgt tattcaaaac
    26821 attgtaatta aagccgttcc cgctagaatg cggcggaatt tgaggacgaa cttatgaaac
    26881 gtttacccgc cgaatgggag ccgcaggatg ccgtactgat ctgctggccg catgaaaaaa
    26941 ccgactgggc cccgtacctg aacgaggccg aagccgtctt tatggacatt gctcaggcgg
    27001 tcgcgcgctt cgaaaaactg atcgtcgtgg tgcccgatga aacatgtatt cacaccaagc
    27061 tggcggcgcg cggcgtcaac atggcgaacg tcgcgcttta tgaaattgaa tccaacgaca
    27121 cctgggcgcg cgacttcggc ccgattaccg tgtatgaaaa cggccagccg gtgctgctcg
    27181 acttcacctt taccggctgg ggcggaaaat tcgaagccgg gctcgacaat cagattaccg
    27241 caaaactgca tgcggcgggc gcattcggca cgactccgct gcgcagcgtc gacctgattc
    27301 tcgaaggcgg cagcattgag agcgacggcg ccggcacgct gctgacaacc gcagaatgtc
    27361 tgctcaaccc caaccgcaac gcgcacctcg acaaacggca ggtcgaagtt ctgctgtcca
    27421 aagaactcgg attcaaaaat tttcactggc tggaaaacgg ctggctggcc ggcgacgata
    27481 ccgacgcgca catcgacact ctcgcccgcc tctgcccgga caacaccgtt ctttacgtcc
    27541 ggtgcgacga cccggcggac gaacactttc ccgccctccg caacatggaa gacgaactgc
    27601 aaaaactgcc ggcataccgg ctcctgccgc tgccgtggcc gaccgcaaaa tttgacgagg
    27661 acggcaaccg cctgcccgcg acctacgcca atttcctgat catcaacggc gcggtgctgg
    27721 ttcccaccta caacgacccc gccaaggatg ccgaggcgct cgccacggta aaaaaggcct
    27781 ttcctgaccg cgaaatcatc ggtatagact gttccgcgct gattctgcag cacggctcgc
    27841 tccattgcgt aacgatgcag attcccgaag gagttctgaa atgaaaaaac tgaaagccgg
    27901 tctggttcag cagtcatgca ccgcagaccg tgaagcgaat attgaaaaaa gcatcgcggc
    27961 catccgccgc tgtgcgaaac agggcgcgaa actggtcgcg ctgcaggaac tccacgccgg
    28021 tctctatttc tgtcagaccg aagacacgca cttctttgac ctcgcggaac ccattcccgg
    28081 cccgtccacc aaaaccttcg gcgcgctggc aaaagaactc ggcatcgtaa tcgtcacgtc
    28141 gctgtttgaa aaacgcgcag cgggccttta ccacaacacg gctgtcgtgc tggactctga
    28201 cggaagcatc gcgggcaaat accgcaaaat gcacattccg gacgatcccg gttattacga
    28261 aaagttttac ttcacgcccg gcgacctcgg ctttacgccg atcaaaacct ccgtcggcat
    28321 gctcggcgta ctcgtctgct gggatcagtg gtatccggaa gccgcgcgcc tgatggcgat
    28381 ggccggcgcg gatattctga tttaccccac cgctatcggc tgggatccga acgataccga
    28441 aggcgaaaaa gcgcgccagc gcgaagcgtg gatcacgatc cagcgctcgc acgcggtcgc
    28501 caacggcatt ccggtgctga gtatcaaccg gaccggcttc gaaccgtcgc cgtgcggtca
    28561 gggcggcgcg cagttctggg gctccagctt cgtcgcgggc tgccagggcg aactgctggc
    28621 acaggccggc accgacggcg aaaccgagct gatcgtcgaa ctcgacggca aacgcagcga
    28681 gtcggtccgg cgcatctggc cgttcctgcg cgaccggcgc attgacgcct acagcggact
    28741 caccgcccgg tttctggatc aggaatcatg acactcgaag aattcggttt tactccgtgg
    28801 tttcagcagc aggttgatgc ggctgcgtcc aaagactgca aaccggcccg catcaccgcc
    28861 attcataaag gctggtgcga catcagcgac ggcgaagaaa cccgtccggc gaaaaccacc
    28921 ggcaaactgc gccgcgccgc ccgcgaaaca aaggattatc tgacggtcgg cgactgggtg
    28981 ctcgcaacca attttgatgc cggcgacttc gccatgatcc attccgttct gaaacggcag
    29041 aatactctga gacgaaaaat ggcgggcaaa gaggtcagcc accagctgat cgccgccaat
    29101 atcgacaccg cctttgttgt tcacacgctc gacaaaggat tcaacctgcc gaagctggaa
    29161 cgctatcttt ccatcgtgaa cgaaagcggc atcgagccgg tcgttctgct gaccaaaaaa
    29221 gatatgctgt ctgccggcga gttaaaggaa cggctggcgg aagtcgaagc ccgtctgccg
    29281 aacgtgccgg tctacgcctt cagcaacacc accggttccg gactgaaaaa agttcagaaa
    29341 cttttcgaac cgggaaaaac ctactgcctg ctcggcgcct ccggcgtcgg caaaacctcg
    29401 ctgatcaaca gcctgatggg cgaagaggat ttatatttca ccctgcccgt ccgggaatgt
    29461 gacggcaaag ggattcacac cacgacctgg cgcgaactga ttgaactgga taacggcgca
    29521 ctggtgattg atacgcccgg catgcgcgaa ctcggccatc tggataccgg cgccggaatt
    29581 aaagacacct tcgaggaact gaccgcgctg gaaggacaat gccgcttccg cgactgcgcg
    29641 cacaccaaaa ccaaaggctg tgccatcgaa gcggccgttg aggccggaac cattccgaga
    29701 gcgcgctacg aaaactttat ccgcatgcgg caggaaaccg ccgccaacga acgcgccctg
    29761 cgccagcaaa catggaaaaa caagtaggga cggctcaccg ggccatcggt ggcgctttcg
    29821 gcgaaagcgc cctacttcat cagctgatca agcagcccgt aataatccga gatcgactga
    29881 acggacttct gcatcagaat ccatgtcgcc caggtgagga tgacaacaac cgcggtccag
    29941 acgatttttt tacgcaggct gaaattcggg tgaagccaga agagcggaat ggcaaacggg
    30001 ccgacgcaaa gcaccgccgt aaccagccag ccgggccgaa gataccacgg aaccctggtt
    30061 ttctgcacca ggaactgtcc gcaaaagcgg catagaaccg cgtcatcctg aatttcttct
    30121 gcgcaaaacg gacatttctt catgcgccgg tattacgcct tccctgttcc gtttacaaga
    30181 ggagcatgtg aatcgtgcat cagcttatta aagaacttcg gtgccggcgc ttcgaagaac
    30241 atccgctttc cgctgtgcgg atgattgaac gaaatcgacc gggcgtgcag cgccatgcgc
    30301 tcgaacttcg tgcccttgag cccgtatttt gtgtcgccga ccaccgaatg gcccgcctcc
    30361 gccatgtgca cccggatctg gtttttgcgg cccgtcagca ggttgatttt caattcgctg
    30421 aactctttcg tctcccggac aacggtgtat tccgtctcgg accagcggcc cttggtctga
    30481 ctggttgaat aaacaaactg cgcctcgttc tccgccagat agctagaaat ttttccggtc
    30541 ttctccctga aatgcccgtg cacaacggcc agatattcct tctccgtcgt cggccactca
    30601 cctttaagct tctgctgcac cgcttccgtc ttggcgaaaa tcagaatgcc ggacgtttcg
    30661 cggtcgagac ggtgaaccgt ataggcgcgc aaccgcgagc gcgccgcgcc tttgcgcagg
    30721 taatccgtca gcagatgctg ggcggtttgc agctcgtcct tgggcgtact catcgtcagc
    30781 agcccggcat ctttattgat caccagcaga tcgcggtctt cataaatgat ttccagccct
    30841 ttcgggcgat gtcggtgcgg tgcgctcata actcattatt ccgccgggtg ggggcacccg
    30901 gcctacaaca tgactcctga aactgtaggc ccggccacct gaccgggcgc tttcatgaaa
    30961 cagacagaac ctttccggat gaaccggcgc ggaatgaaaa tttcgcgccc gtctttttat
    31021 ccagcaatgc cgcgccgacc ggcgactccg gcgtaaccgc cgtcacgtcc acgccgtcaa
    31081 cacggacttc cgttccgccc gcgcaaggca gcagaaaaaa cgtcatcttc tctttatcca
    31141 gctcgacgtc caccagcgcg ccgacgccga tcggcttgcc ggaaaaaacc ggaatcataa
    31201 acgtccgcag ggtttcgaga tcggcaacca gcgctttgaa ctggagcgca tgcccgcgcg
    31261 ccagatagga tgattccagc ccgcaggtat cccacttggt ttccgcgcgc gcctcgccgt
    31321 gcgtagcgcc ggccgacgca tcttcattcg ccgcctgctg acggcggaga tcgttttcga
    31381 gctcggtaat aatggcgcgt aaaagagcgg gtttattcat gggtttccga taatggcgga
    31441 tgatattcag tctgtgatca aggcaggctg gaagcctgcg gtacgtatcg ccggcttcca
    31501 gccggccctg tctttaaacc gagaatcttg cgtcttcgtt tacggatcga gattttcgag
    31561 cgccagcgtg ttgagatcga tcttgcggta atagcagttg cgcggaacgc ctgcgccgtt
    31621 cttggtgtgg cagatgccgc cgcggttcgg acggacgata aacagcagcg agttctgctc
    31681 gcagttgacg cgcgcttcca gcagttcaaa cgtttcgccg gaggtcagcc ctttaatcca
    31741 cagctcctgc cgcgacgaac tccagagaac cagcatcttg cgcttcagcg attcctcgaa
    31801 cgccagctgg ttgatgtaag cgaccagaat cacttccttg gtatcggcgt gctgaacggc
    31861 cacgggaata atccctttgg taccggcaac cgccttctcc agcttgttcc agtcgagttc
    31921 gagtttcagc ccttcttcca attccttgct catctcttcg cccttttccc tgttttataa
    31981 atttcgacgt ccctcacttg tagggcgggg tccgtgaccc cgccggagac cgaggccacg
    32041 ggcctcggct acaat
//
LOCUS       DAOPZO010000009        28443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_1649_length_28443_cov_9.233338, whole genome shotgun sequence.
ACCESSION   DAOPZO010000009 DAOPZO010000000
VERSION     DAOPZO010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 28443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28443
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1649_length_28443_cov_9.233338"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..483)
                     /locus_tag="PKI68_01385"
     CDS             complement(10..483)
                     /locus_tag="PKI68_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HNX52377.1"
                     /translation="MILDRIENRSLYPFGTAWKAAFDFLKTLTPDSADGKHLVQGNEL
                     TVAINTYTTKARAAAKLETHRKYVDIQLLLSGSEVIEVFPKAGLTVSEPYIPERDVEF
                     YQVPAGAPARMTLVPGEFLVFFPDDAHMPCVMAGNSPEPVKKVVVKIAVELLTQK"
     gene            692..2905
                     /locus_tag="PKI68_01390"
     CDS             692..2905
                     /locus_tag="PKI68_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNX52378.1"
                     /translation="MHRCRVVVLSTVLLFLYSFAQDTPVEDSGAAPAPAVPAGNSGRS
                     TFKEKMAQRRASGATDQAVTTQRPAEGVQLETTRTAAPEASQVTVNWENVTLKDCIEV
                     LCRDLGMEFVISPSVNVAQEVSVRAGDVTRWTAQDKQELFDAILETAGVQRVQRGRVW
                     VFSPSDIRPVVEAFKDAKYGDGKPVIGMIKLQNIDASQAEPFLNTVGGKPQRVFAMKG
                     SRMVLVLGTQQFLTQMESLVKLIDFPSGMIRPFPLKYASSDELAKEMTAIFAGRAGSD
                     GETVRFVSVPRLNVVVAQNIPEAMLEDVTKWIGLLDREDNANERVTRVYRLQVIDAET
                     IGKTLDSLYSTLYKQTQAQVKQQAGAAAKKTAAGNSTVKSAPAPTPAGGGGAAAAPKA
                     ETASAAKSAGSDAGSPDMQEEAVILADKDTNTLIINATPDQHRDIEKTIKELDRARRQ
                     VLIETVLVEVTLDESLDLGVEWAVAKGFSSAGLQSGLDSKISGLNDATADNGFSYLIQ
                     SSEQKWAFIQAAENDDRLQVLSSPTVLTRDGMPATVSFGKEVPVQEKSTSGNSSTRDN
                     YSYNYMDAKITLTVTPVIDDNKMVTLNLSQVLRQLPPDYKLSADAQDNVIPPIFTTRE
                     LSSNLQVDNGQTLVLGGLIQREDQKVRRGIPFLCNMPYIGWLFGRTVTVKKGSEILMI
                     LTPHVVDTREETDLLTRDFRSKILGAMSSKNIRKVYGVEEIKKPAEPAATPAEGK"
     gene            2907..4595
                     /locus_tag="PKI68_01395"
     CDS             2907..4595
                     /locus_tag="PKI68_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNX52379.1"
                     /translation="MAYRQLGDILVAQGAVSRDEVQAALRAQQADVGKPRLGELLVQS
                     DKITSAALAAALAAQADLPLCACEQPVDHLFLNENLPFAFLVEHRILLLAGAAGQAAS
                     AITDDPSDWSAIEAVRLSYGQQLPVAVVSAEQMDAALEKIRSFADSNLNTLMKDAEIV
                     EAGDWSDEEEHLRDLALEVPIVRMVNLIITQAVERRASDIHVEIGEHRLRIRYRVDGV
                     LYEAEQIDKKHHAAVVSRLKLLARLDIAEHRIPQDGRIKMQVQGHDLDLRVATTPTIY
                     GENVVIRLLNQQQVELSFSSLGMPLREQQGFTRMIEEPQGFILVTGPTGSGKTTTLHT
                     ALTHLNSEKVKIITIEDPVEIRLPSINQIQMNPKIGMTFANGLRSIVRQDPDIIMVGE
                     IRDSETADVAIQASLTGHMVLSTLHTNDAAGSIARLAEMGVEKYLIASALSGVLAQRL
                     CRRICPHCSEPYTVSGTHLVDFPEVTEASYTLHRGKGCEYCDGTGFRGRMGLYELLIV
                     DDEMRSLIQRTAEAGPIRQLARSKGMRLLRESGWDAVVSGASTVEEVRRVTRWG"
     gene            4600..5814
                     /locus_tag="PKI68_01400"
     CDS             4600..5814
                     /locus_tag="PKI68_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNX52380.1"
                     /translation="MMRFSYRAVTSDGTPRNGSLEAPGKAQAVQSLQQSGLVVLSLSA
                     AGEPGASGGGSRALTGRISAPQLVDFAIETSALLDAKVPLEQVLKTQADLCTHPRFKE
                     VLTAVWQDVNGGASFADALARHPKIFERFFVNMVRGGEASGALELIMRRVAALLERRM
                     QLRSKVTGALIYPSLVMVLGVAVVSGMMLFVVPKIISMVGESGQLLPVSTRAVIAVSN
                     FTRSSWWLFPLAVVGLFYLYKMKTRSEEGKVAFGRMILKIPLFGTLIAQAETSRFCRM
                     MSSLLDGQVPILSALSITGSTLGNAALRQLMKEVYSRVQGGQPMGPLLQASPEFPELA
                     SRMITMGEESGELQNMLNKVADRYEEKVTATTERLVGVIEPVSIIIIGLIVGFIVVGM
                     VQGIMTMSTSTG"
     gene            5843..6277
                     /gene="gspG"
                     /locus_tag="PKI68_01405"
     CDS             5843..6277
                     /gene="gspG"
                     /locus_tag="PKI68_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNX52381.1"
                     /translation="MKKRKQAKQGFTLVELLVVITILLIIGTFATQQVINEPDKAKVK
                     VTKASFTSLENALERFKLDVGRYPTQEEGLSALYTAPDGVENWAGKYLTKSRALRDAW
                     DNDYVYIIPGKNNEPYEIISLGADKAEGGEKYNADLSNLDEK"
     gene            6295..6915
                     /locus_tag="PKI68_01410"
     CDS             6295..6915
                     /locus_tag="PKI68_01410"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNX52382.1"
                     /translation="MCRTPNSGFTMIELLVVLAIILVMTGLAVGTLSGSGTPKQQLRR
                     EARDLTALLQEARLSAMERKLKIEVYINPPARTVYAVESGYARKLLAADDGFFSETAA
                     PDGFVPETNRFFRTAVFPETIAIEPFALQEIEPETPGTEPLFEAAPEGTGDTDAAFLN
                     RPVFSFTSLGGATGGGISVVRGEVRIDIACDLLTGLPEIVRRRAIK"
     gene            6912..7307
                     /locus_tag="PKI68_01415"
     CDS             6912..7307
                     /locus_tag="PKI68_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNX52383.1"
                     /translation="MKKAGFSLLEVLVALAIFAIAAGGLLVALGNHLKNVSFMQDHAR
                     AVRIAAREMNALRRMTTFAEGETDGSEDRFVWTKTVSADGLDTLPGLTSATGTPARLS
                     VTVKWSDRPGGLPLGKVQLDGLTVFGAAE"
     gene            7307..8050
                     /locus_tag="PKI68_01420"
     CDS             7307..8050
                     /locus_tag="PKI68_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNX52384.1"
                     /translation="MRCFSIVHRSAARRGFTLIELLVALALLGILGASLAGVLRNSMD
                     SVEQAQSSVDHLTRLRSLDTMLGAALRDATALALSTNEQKLLSAQGSFDAAAGTIRFH
                     GEERLLGFCLQRPFFSAERDGHMHWVTLEVRQDEKTGRSSLWLQDISFIPGLDNPVGA
                     DWGGLEAEVKERLPVQEVCLIREAAALSFKYWLAPADASSDAEEVEADSIAGDYALAV
                     PDYIELSIQLPKGAPDVLTFDYSFSRRVL"
     gene            8050..9096
                     /locus_tag="PKI68_01425"
     CDS             8050..9096
                     /locus_tag="PKI68_01425"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HNX52385.1"
                     /translation="MNDFPGNPRSGSALLIVLGVMAILSLLVAGFGRGLRADLRASGG
                     FYEAEQNRQLALSALAASQVERAQTLLYADATGNVYFVSSDDTAESEIAALSAYRQGL
                     PLGRGMLSYRFVMKPAALDLNSLDAAALGRLFEVACGMPESDERSALADAILDWADAD
                     SLPRAYGAEEEVYQALVPPRHCRNAPFETLEELLLVNGITPDLLFGYSHPVSIENGLL
                     LGGGLYRFLIGDNSPEAQASVQYILRGVLPDDAKAAEPPAEGFTKVTVLPPVLYLVAE
                     GFMPAAPAETKTASTGEQPVEAASRHTILVKLILPDTAQAKTYTVADFQENAAGELLA
                     QVLAYGIPEETNEL"
     gene            9086..10477
                     /locus_tag="PKI68_01430"
     CDS             9086..10477
                     /locus_tag="PKI68_01430"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNX52386.1"
                     /translation="MNFKKMPEMKWIAGNSRLLLAFQDSTVDAVWLEQSLLGPSVRMM
                     ERFPRDEQLPGRLVQRLQAAGKTPARVTVCLPRSMVMQRTLAYPLAVQDDLAQMVQFE
                     ASRHVPLPEADRRIAFAAAPLPGGKQLGVNLLAARTSEISALIDPLVAAGLPVDEVTG
                     LSSLLVPADPRLAVLLILTDDTQLELALFAGGLLQDRLLLNRQAAGFGKETLVDAVRR
                     MTVRHHALLGAEGIGRVVCGGVSALPDEFQHSLEATFGLHIHALEVPPALQSALPPGA
                     PLLAAALAAAVSEFAPSLNLIERSGRKVSLSRRTLIVAGLCALLAIELLAGGLLRTFA
                     PAVALKAAEREAAELKRRTAPIQAVKSSNRALRSELEQLTDLGKTRVSLMQMLKVLSD
                     TLPEDTYLESISYTRGDDIKLKGRSKTPDRLPQLIQSLPFVKTLEASDIGEKNDDYFG
                     FTISAALRSVRDE"
     assembly_gap    10628..10727
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10728..11018
                     /locus_tag="PKI68_01435"
     CDS             <10728..11018
                     /locus_tag="PKI68_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52387.1"
                     /translation="YPEILNLVYQLEIHRPLIVVDRFSLSVQSGRRSFGQNNRPSGGP
                     SGSTGFSGSGRSGFFSSQFSRRSTAADTPSEPRMRLQMDIHINVRPSEGGAK"
     gene            11015..>11784
                     /locus_tag="PKI68_01440"
     CDS             11015..>11784
                     /locus_tag="PKI68_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52388.1"
                     /translation="MKMIAPRYQIPLLIGLCALAALALIPAVHIPSPAAASRAGAAAP
                     VPTGPVTFNDTPPTMHEVAAKHLFIAQRKATGQKSFPDLVVKGIFIGAEKSVVLSLKS
                     RPQANLRVWEDGSDRALSQITKPDDPRQPLAAFLREWKVKEISFNGISVEHQITGEVE
                     TYAVDYTPEKKVKDDASRGYGQGIIPQGGDGGPSDSVRTAGSNTSRPVQPQQQVTAPP
                     VTQMADRISEMVQRMTPDQRKQFLQRVQQNMTGGNQNN"
     assembly_gap    11785..11884
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11885..13757
                     /locus_tag="PKI68_01445"
     CDS             <11885..13757
                     /locus_tag="PKI68_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX52389.1"
                     /translation="LSGLRGAVIRQKCRIRSLKHTEDDVRDSKGSSSKSGYNRFLLLV
                     AGLGGLLYGIDVGIISGALPYLEATFKDSATGQSLTAGNLSFIVAAVLLGSVISTLFA
                     GVLSDWMGRKKLMFLSGLLFVVSIPVIALSSGYSSLVLGRLLQGVSAGLIGVVVPLYL
                     AECLGAANRGKGTGVFQWLLTLGIVAAAGIGYYFSQEVAQIEKLQDAAKLFAFKDTAW
                     RSIFWVSLPPGLLFVIGSLLVAESPRWLFRKGRKEAALAALLRSRTDEQAHNELREME
                     ETAAAEKAQTNSAGAKIKESLLRRKYVIPFVLACVILACNQATGVNSIIGYNATILIQ
                     AGLSDANAHLGYVILTLVNFLVTMAGVALVDKKGRKFLLSLGSSGIIISLIFAGLVFH
                     KNEKARVDVKEAVQAMVIESNQTASIDFNNEVAAKLMADKGGAANRQSATMVVIYSYG
                     DFHAASKVVRSDDIAAKIEISRASCVPVNKVAAFFQNPFADLDAARVAPLKIDNALIT
                     PVPSEKSGWLTAICIFCFMAFFAIGPGVCVWLALSELMPTRIRSNGMSIALLVNQAVS
                     TSIAAVFLPTVGKYGYGTMFFVFAGCTVVYFITATFFLPETKGKTLEEIEEHFEGGK"
     gene            13821..16643
                     /locus_tag="PKI68_01450"
     CDS             13821..16643
                     /locus_tag="PKI68_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNX52390.1"
                     /translation="MKKRSIYKILATLLMSGAVFAAPIDPAIDDAPGPFCYFSRPSFY
                     FTVADHDDGTQVTPEGWLWTGSAQMLFFAGADLAPLRERIQTLREPGVPVVVQTSRIG
                     DIVYEITRFGATLDGNPQSALVNFIRVRMKNEGKQPGEATFAVAVRGKGDHCCERLTT
                     AWNPVAAQYEMGANCAVRDGNLLYMFPVDPPPKRFVVPDAENSGPVKALDEYVGLAEP
                     VCMVRYGLDLKPGEEKALEFKMPFKPVSVKDQKTVDALAGASFDKYLKQTEKWWHEFH
                     ANGMQIELPETKVTDTFQASIMFDTMAREKIGDDYIVTVTHLNYHDYYLRDGAYITHA
                     LDLTGRRQWVEQGLEYMLKRHEPDGRIVEKPAWDGYGQAMWAFGAHWRLTGDNEWARN
                     VYPNLARHVRAVFAQVATDPLGLVPVAPPYDNEGINGHYTGHSFWMLIGMADMIAMAE
                     ALGEKADAEQFKQWRDDYKANFTKALDAATAKTGGYIPPGLDADNGNDWDNLISLYPQ
                     GGVPAKGALDPADPRIGKTLDMVRKNQYREGIMTYGRGLKAGALHHYLTFKATEGLVA
                     LNRQREALEDLYGVLAHTSSTGAGFEFKPGADGVWGNRDTEGNYPCHGWFAAEYIGLL
                     RNLLVRESAGDLHLFSVLSPEWVKPGSRIAVRNAPTDFGMISVVAEIKDRAMTVNLDT
                     QWRTPPRSLIMHLPWYLDGAAATVDGKPAVISKPPYGEGQQIVLPPKARTVTVQWKPA
                     GLPELSFANTVAQWKKQNRQHYDEYVAQGGKPELLWKEPALAMTRAERQKGWIAEKGI
                     AVGCKTAASGFEEGHQPENAADGRVHRPTEWSVKTAPAWWQVDLGKAQLIDSVRVISA
                     WEPNKEKLSWQYKVLVSTDGSQWQTVSDYSNNQKTETSKGTLHTFQPVSARYVRVEML
                     GNSKKIPASSLNEVKVFPAMPPAAEF"
     gene            16876..18267
                     /gene="thrC"
                     /locus_tag="PKI68_01455"
     CDS             16876..18267
                     /gene="thrC"
                     /locus_tag="PKI68_01455"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNX52391.1"
                     /translation="MKYISTRGQMEPVGFLDAVMTGLAPDGGLLLPESLPDVSRKLPV
                     WAGLSYQELAFEVMSLFATDLPPADLKVLIEKSYSTFRAPEVAPVIPVDGFHVLELWH
                     GPTLAFKDVALQFLGNLFEYVLEKRGPSASSGQAAELNILGATSGDTGSAAIYGVHGK
                     KNIRIFIMHPKGRTSPVQEKQMTSVLDDNVFNLAVEGTFDDCQHLMKSIFSDVEFKEK
                     HSLGSVNSVNWARVLAQIVYYFYAAFRVQENTGAAQVQFAVPTGNFGDIMAGYIAVQM
                     GLPVKRLILATNENNILSRFFNTGVYSIGEVVPTISPSMDIQVASNFERYLYYRVGQD
                     AAKLRDLMAGFAKTKTLTVPGTDELFVAGEAGTAQTLAMIKKCYEKEGYILDPHTAVG
                     VAVAERFADSAVPTICLATAHPAKFNDAIQQAIGIAAHHPALDKLANAETRCDTIAND
                     EAAVRDYLIAKTK"
     gene            18279..19259
                     /gene="trpS"
                     /locus_tag="PKI68_01460"
     CDS             18279..19259
                     /gene="trpS"
                     /locus_tag="PKI68_01460"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNX52392.1"
                     /translation="MADKKNMRILSGIQPSGKLHIGNYFGMMKPAIELQEQGEAFLFI
                     ADYHALTTVNDAAALRTMVKDVALDFLATGLDPEKTAFYRQSDVPEVQELAWLLSVVT
                     PMGLLERCHSYKDKLAKGIAATHSLFAYPVLMAADILSVQSTVVPVGRDQKQHVEVTR
                     DIAVKFNNQFGEVFTIPEPSIRDSVAVVPGIDGQKMSKSYGNTLEIFGDPAALKKRVM
                     QIVTDSTPLEEPKNPDTCNAFKLYQLFASDAEAADLAARYRAGGFGYGTAKKELLAKI
                     NEHFAPMREKRAELEKNPDFVNDVLKKGAERARAETRKTLQAARKAVGLE"
     gene            19265..20044
                     /locus_tag="PKI68_01465"
     CDS             19265..20044
                     /locus_tag="PKI68_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNX52393.1"
                     /translation="MPDLLPNEEYKVSLEVFEGPLDLLLYLIKKDEVDIYDIPIGRIT
                     TQYMEYLELMKILNLEIAGDFIVMAATLMVIKSRLLLPDEARGPVEEDDEDDPRWDLV
                     RQLVEYKKFKDAAGFLEHLEAVQENVFLREGEHVELGARPEIGMQDVSIFDLISALNQ
                     ALDRVPKEELNEIFAEQYTVSEKVDYLLEATKNGKPLTLGRLFRGMRSRHEIVCTFLA
                     VLELIKLNRLAARQDNHFEEIVIEQVAGGEPEPVLQEEETV"
     gene            20041..20742
                     /gene="scpB"
                     /locus_tag="PKI68_01470"
     CDS             20041..20742
                     /gene="scpB"
                     /locus_tag="PKI68_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNX52394.1"
                     /translation="MSDLEVLPELKEIIGALLFAAKKPLTAKEIRKALLDAGASYGGP
                     YQEFSEFKDDDILAAIEDLKTGLVQSGSGLCIAEVAHGFRLQNKISCGPWVRTLLDKD
                     RTARLSKPALETLAIIAYRQPILRSEIESVRGVAVDQVLRNLVEMQLVKVTGRSELPG
                     RPWLFGTTQRFLEHFGLRSLEEMPGMEELKRMVPVEKAERTKPFPEMEKELEQREEQA
                     VTEESDESGEAEEAD"
     gene            20711..21772
                     /gene="pheA"
                     /locus_tag="PKI68_01475"
     CDS             20711..21772
                     /gene="pheA"
                     /locus_tag="PKI68_01475"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNX52395.1"
                     /translation="MSLEKLRKQIDGLDEELIKLLNERIKIALEIGKTKKEQGGEIYV
                     PSREKAVFERIARLNAGPLPEESARAIYREIMSAALALESNLKIAYLGPPATFTHQAA
                     HAKFGASVDYHPVGTITDVFNAVEHGSADYGVVPIENSIEGAVTHTFDQFTGTTLKIC
                     AEIYLPVSLYLLASVPKENITRVCSKQEAIGQCRRWLSENLSGVELDAVNSTALAAEM
                     ASTGDCAAIASLLAAELYNLNIIAENIQDSSGNTTRFLVIGRKYGEPTGEDKTSIYFG
                     IKDEVGALHDALDALKLNGINMTKIESRPSRSKAWQYYFFVDFEGHADDPHVKTALKA
                     LEKHCAVLTVLGSYPKADD"
     gene            21793..22905
                     /locus_tag="PKI68_01480"
     CDS             21793..22905
                     /locus_tag="PKI68_01480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX52396.1"
                     /translation="MSDILKISVGVVAWNEESSIALALESILKQSIFRNARSLDLELE
                     LIVIANGCTDRTVEVTDDFFAKARSEFPEGSAVSLRLEIVPEAGFANAWNLLAHTYSR
                     PDADFIYFVNADIVIDQPDAFEQMLRGLQRDYAMRVISPVGHKHIEAAKCKTLCDRLN
                     LAVTRFTEKNQTAQVRGHCFCARAAAMRMVWLPKYFPGAVDGCFKRMIVTDMASAEGH
                     PARIGIARNVSYTFEAYRTFREIYFRRVRLFIGQTFIETLMRHLKTMRDEQPKLNPAA
                     YLRARDETDPSWLHRLVQLNIHVNGLFETMPSPWFRFRFWKNPGLSRFKKLGMLPVAV
                     TAFVFDVPVFLSAAWRLKTGRVADVWKDTRNRELRT"
     gene            22927..23331
                     /locus_tag="PKI68_01485"
     CDS             22927..23331
                     /locus_tag="PKI68_01485"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNX52397.1"
                     /translation="MPSHTLQFRVRYSETDQMQTFYNSRALEWFEVGRTELSRAVGVP
                     YSEWERRGLLMPLVEARVFFRGRAGYDDLLEMRVTLKAEGRARLLFENHIVQAGTGAP
                     VADGYTVHAMVSLETGRPVRAPDWVVELLNKE"
     gene            23335..24915
                     /locus_tag="PKI68_01490"
     CDS             23335..24915
                     /locus_tag="PKI68_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNX52398.1"
                     /translation="MERQFLMGNEAVALGALHAGAARGAGYPGTPSSEILDVFNQLGG
                     KAEWAPNEKVAAEVALGVSFAGVRAFTTMKHVGLNVAADVLFTAAYSGVDGALVFIVA
                     DDPGLASSQNEQDTRRYAVAAGVPLVEPSDSQEAYDFTRLAFEISGRWKIPVIVKLTT
                     RVCHSGTIVVPRKPDAGLPEAHFERNIPYHVMVPGHARPAHRRLRQKLAEIAEWNEKE
                     GPNQKVKGSKDLGILVSGVAYQHAREAAPGASFFKVGMSYPLPMRQMLDFAASVDRAF
                     VVEENDPYMADAVRAAGGTIESAPEMYRFGELDVGRVKRILTQDATPEAVPPRGKPPQ
                     LCAGCPHTITFEMLKKHNCIVAGDIGCYTLAALQPLAAMDTQICMGASLGVGLGLRHV
                     LPREEAKRVVSVIGDSTFVHSGLTGLAEMTYNVPPTGHVLLILDNATTAMTGQQEHPG
                     TGRLLDHTPANRLDYEKIVRAMNVQNVGVFDPVTQRDGYEAALTQALEKDELTVLIVR
                     RPCILAVARKGVKPATQE"
     gene            24921..25397
                     /locus_tag="PKI68_01495"
     CDS             24921..25397
                     /locus_tag="PKI68_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNX52399.1"
                     /translation="MSDKVTSIKIAGLGGMGVLTSTQILAEVFFRQGLDVKKAEVHGM
                     SQRGGSICSDVRFGKKVFSPMVPAGSLDFLVLFQEDQFPLYEADCAPGTLILKPAAID
                     LAKLENKKALNIAMLGLLSRHLDVPADVWLEVIREVLPAKLHKVNEAAFELGRKSV"
     gene            25432..25932
                     /locus_tag="PKI68_01500"
     CDS             25432..25932
                     /locus_tag="PKI68_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNX52400.1"
                     /translation="MGLVKHFRDLRVYRTAFDAAMQIFELSKKWPSEERYSLTDQIRR
                     SSRSVCEQIAEAWRKRRYPAHFRSKLTDADSEAAETQSWLEFALRCGYITKDQFVSLD
                     AVYESINGGLVRMIDQSEQWCVFQPKVEEEGCDYLLSEQLTHSDTPALTNTPDGSRAE
                     DDFTDE"
     assembly_gap    26031..26130
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <26131..27030
                     /locus_tag="PKI68_01505"
     CDS             <26131..27030
                     /locus_tag="PKI68_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX52401.1"
                     /translation="TGGLGAHYGAERLGATVIPISGGNTERQILVMKDFGVTAICCTP
                     SYFIHLIEKINASGISFRELGVKIGVFGAEPWTEAMRTYIQRESGIKAFDIYGLTEII
                     GPGVGIECCEQAGLHIFEDHFLMEIVDPETDEVLPDGQEGELIITTLTKQAFPMIRYR
                     THDITSINPERCACGRTIRRINRIRSRSDDMMIIRGVNVFPSQIEAALLSVEETVPHY
                     QILLTRKGGLDQIEVQVEVTKEMFGDRISELESMQMRLMHAIDHITGLRVPVRLVEPH
                     TIARSEGKAKRIVDKRSEGEEIR"
     gene            27027..27455
                     /locus_tag="PKI68_01510"
     CDS             27027..27455
                     /locus_tag="PKI68_01510"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNX52402.1"
                     /translation="MKIKQLSVFLENRPGHLSRVCEVLAEAGLNIVTMTLADTLEFGI
                     LRLIIREWSRAKTVLEAAGYVVNVTDVMAIEVTDEPGGLEKILKTAETGGLSVEYMYA
                     FACKQNKNALIVIRFDNPERAAAVLAEAGIGILSAAEFYA"
     gene            27466..>28443
                     /gene="hisC"
                     /locus_tag="PKI68_01515"
     CDS             27466..>28443
                     /gene="hisC"
                     /locus_tag="PKI68_01515"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNX52403.1"
                     /translation="MISNKWIETLPVYEPGKPIEEVARELGFADISEIIKVASNENEL
                     GPSPKAMKAMAAAATEMHRYPDGGCFYLKEKLAGQLGVKPQNLLFGNGSNELIEFLGH
                     VYLGAGTNLVMSEGAFVVYRLVAMLFNAKVIAAPMKKFGHDLDAMLAAVTPETRMVII
                     CNPNNPTGTIVTPAKIKAFLKKLPANVLAVFDEAYFEFMPDDQKPDLIKEIQKGRENV
                     IVLRTFSKAYGLAGLRIGYAVGHEKLIALLNRVRQPFNVNSMALAAAFAAFDDDKHLE
                     KTRKTNFQGLELFAKELPKIAGGLEMVPSYANFFLVKTGNGRQVFGELQK"
BASE COUNT         5836 a         7937 c         8725 g         5645 t
ORIGIN      
        1 gttctgcggt tatttctgcg tcagcagttc gacggcgatt ttgacgacga cttttttgac
       61 gggctccggt gaattgcctg ccatgacgca gggcatgtgg gcgtcgtcgg ggaagaagac
      121 gagaaactct cccggcacca gggtcatgcg ggcgggggct ccggccggca cctgatagaa
      181 ttcaacgtcg cgttccggaa tgtagggttc gctgacggtg agtccggctt tggggaaaac
      241 ttcgatcact tcgctgccgc tgagcaggag ctggatgtcg acatatttgc ggtgcgtttc
      301 cagtttggcg gcggcgcgcg ctttggtcgt gtaggtgttg atcgcgacgg tcagttcatt
      361 gccctgaacc aggtgcttgc cgtcggcgct gtccggcgtg agggttttga ggaagtcgaa
      421 ggcggctttc caggcggtgc cgaacgggta gagagagcgg ttttcaatgc ggtccagaat
      481 cataaccatc ctttttttgg taaatctgac atttgccagc gcgcaacata atgaagttta
      541 cccgcgggct gaacaggaaa tatttgtgct tttttaaaat aaacaggtag agtcccgcgt
      601 tatcgaaggg tcgcggtgaa tttatgcctg tggtttttcc gcaggttttg ttcgttttgc
      661 cagaaatcgt ttaaaacagg tggggtgagt tatgcatcga tgccgggttg tggttctgtc
      721 gacggttttg cttttccttt actcctttgc tcaggacacc ccggttgaag attccggcgc
      781 agcacctgcc ccggctgtgc cggcaggaaa ttcaggccgc agcaccttta aggaaaagat
      841 ggctcagcgg agagcgtccg gagcgactga tcaggcggtg acaacccagc gtccggccga
      901 gggagtacag ctggaaacca cccggaccgc cgcgccggaa gcctcccagg tcaccgtgaa
      961 ctgggaaaat gtcaccctga aggactgtat cgaagtgctc tgccgcgatc tgggtatgga
     1021 gtttgtgatt tctccgtcgg taaacgtggc gcaggaagtc agcgtgcgcg ccggcgatgt
     1081 gacccgctgg acggcgcagg ataagcagga gctgtttgat gcgattcttg aaaccgccgg
     1141 cgtgcagcgt gtgcagcgcg gacgggtctg ggtcttttcg ccgtcggata tccgcccggt
     1201 cgtcgaggcg ttcaaggatg ccaagtacgg cgacggcaag ccggtgatcg gcatgatcaa
     1261 gctgcagaat atcgatgcct cgcaggccga gccctttctg aatacagtcg gcggcaagcc
     1321 gcagcgcgtc tttgcgatga agggctcgcg gatggtgctg gtgctcggca cccagcagtt
     1381 cctgacgcag atggaatcgc tggtcaaact gatcgatttt ccgtccggca tgatccgccc
     1441 ctttccgctc aaatacgcat cctccgatga gctggcgaaa gagatgaccg ccatttttgc
     1501 cgggcgggcc ggcagcgacg gcgagacagt ccgttttgtt tccgttcccc ggctgaatgt
     1561 ggtggtggcg cagaatattc cggaggccat gctcgaggat gtcacgaaat ggatcgggct
     1621 tctcgaccgg gaagacaatg cgaatgaacg cgtgacgcgg gtttaccgcc tgcaggtaat
     1681 cgatgcggaa accatcggca agacgctcga ttcactctat tccacgctct ataagcagac
     1741 tcaggcccag gttaagcagc aggccggggc cgccgcgaaa aagacggccg ccggtaatag
     1801 caccgtcaaa tcggcgccgg cgccaactcc ggccggtggc ggtggcgcgg ctgcggcgcc
     1861 gaaagccgaa acggcttcgg cggcgaagag cgccggttcg gatgccggct cgccggacat
     1921 gcaggaagag gccgtcattc tcgcggataa agacaccaac acgctgatta tcaacgcgac
     1981 gccggaccag catcgcgaca tcgaaaaaac catcaaagag ctggatcggg cgcgccggca
     2041 ggtgctgatt gaaaccgtgc tggtggaggt gactctggat gagagcctcg atctcggcgt
     2101 cgagtgggcc gttgccaaag ggttttcgtc cgctgggttg caatcgggtc tcgatagcaa
     2161 gatttccggt ctgaacgatg ccacggcgga taacggattc tcctacctga tccagtccag
     2221 cgagcagaag tgggccttca tccaggcggc agaaaacgat gatcgcctgc aggttctctc
     2281 ctcgccgacg gtgctgacgc gcgacggcat gcccgccacg gtctcgttcg gtaaagaagt
     2341 tccggtacag gaaaaaagca cgtcaggcaa cagcagcacc cgcgacaact attcctacaa
     2401 ctatatggac gccaagatca ctctgacggt gacgccggtg atcgacgaca acaagatggt
     2461 cacgctcaac ctcagccagg tgctgcgcca gctgccgccc gactacaagc tttccgccga
     2521 cgctcaggac aatgtgattc cgcccatctt caccacccgc gagctgagct ccaatctgca
     2581 ggtcgacaac gggcagacgc tggtgctcgg gggcttgatt cagcgcgaag accagaaagt
     2641 ccgccgcggc attcccttcc tgtgcaatat gccgtacatc ggctggctct tcggccgcac
     2701 ggtgacggtg aagaagggct ctgaaattct gatgattctc acgccgcatg tcgtcgatac
     2761 gcgcgaagag accgacctgc tgacccgcga tttccggtcg aagattctcg gcgcgatgtc
     2821 gtccaaaaac atccgcaaag tgtacggcgt cgaagagatc aaaaaaccgg cggaacccgc
     2881 cgcaacgcct gcggaaggga agtagtatgg cctaccggca gctgggcgat attctggtgg
     2941 cgcagggagc tgtcagccgc gacgaggtgc aggccgccct gcgggctcaa caggccgacg
     3001 tcgggaaacc ccggctcggc gaactgctgg tgcagtcgga taaaattacc tccgcggcac
     3061 tggccgccgc gctggcggcg caggccgatc tgccgctgtg cgcctgcgaa cagccggtcg
     3121 accacctgtt cctgaacgaa aacctgccgt tcgcgtttct ggtggaacac cgcatcctgc
     3181 tgctggccgg cgccgccggc caggcggcct cggccattac cgacgatccg tccgactggt
     3241 ccgccatcga ggccgtccgg ctctcctacg ggcagcagct gccggtcgcg gtggtctcgg
     3301 cggagcagat ggatgccgcg ctggaaaaaa tccgcagctt tgccgacagc aacctcaata
     3361 cgctgatgaa agacgcggag attgtggagg cgggcgactg gagcgacgag gaagaacatc
     3421 tgcgcgatct ggcgctggaa gttccgattg tccgcatggt caacttaatc atcacccagg
     3481 cggtcgaacg gcgcgcctcg gacattcatg tcgagatcgg cgagcaccgc ctgcgcatcc
     3541 gctaccgggt ggacggcgtt ctgtacgaag ccgaacagat cgataaaaaa caccacgccg
     3601 ccgtcgtctc gcgcctgaag ctgctggccc gcctggatat tgccgagcac cgcattccgc
     3661 aggacggacg cattaaaatg caggtgcagg ggcacgacct cgacctgcgc gtggcgacca
     3721 cgccgacaat ttacggcgaa aatgtcgtca tccgtctgct caaccagcag caggtggaac
     3781 tctccttcag cagtctcggc atgccgctgc gcgagcagca gggctttaca cggatgatcg
     3841 aagagccgca gggattcatc ctcgtcaccg gaccgaccgg cagcgggaaa accaccacgc
     3901 tgcatacggc gctgacgcac ctgaacagcg agaaggtgaa aatcatcacc atcgaagatc
     3961 cggtggaaat ccgcctgccg tcgatcaacc agatccagat gaacccgaaa atcgggatga
     4021 cctttgccaa cggcctgcgc tcgattgtcc gtcaggaccc cgacatcatc atggtcggtg
     4081 aaatccgcga cagcgaaacg gcggacgtgg cgattcaggc gtcgctgacc gggcacatgg
     4141 ttctgagtac gctgcacacc aacgacgccg ccggcagcat tgcgcggctg gcggaaatgg
     4201 gcgtcgaaaa atatctgatc gcttcggcgc tgagcggcgt gctggcgcag cggctctgcc
     4261 gccgcatctg cccgcactgc tccgagccct acacggtttc cggcacccat ctggtcgatt
     4321 tccccgaagt gacagaagct tcgtacacgc tgcatcgcgg gaagggctgc gaatattgcg
     4381 acgggaccgg tttccgcggc cgcatgggtc tgtatgagct gctgatcgtc gatgacgaaa
     4441 tgcgctcgct catccagcgc acggcggaag ccggaccgat ccgccagttg gcccgctcca
     4501 aaggcatgcg cctgttgcgc gagtcgggct gggatgcggt ggtttccggc gccagcacgg
     4561 ttgaagaggt gcggcgcgta acccggtggg gatgatgcta tgatgcggtt ttcctataga
     4621 gcggtcacgt ccgacggaac tccgcgcaac ggttcgctcg aagcgcccgg caaagcgcag
     4681 gcggttcagt cgcttcagca gagcggactg gtggtgctca gcctgtccgc cgccggcgaa
     4741 ccgggagcct ccggcggagg cagtcgcgca ctgaccggcc ggatcagcgc gccccagctg
     4801 gtggatttcg ccatcgaaac ctcggcgctg ctcgatgcca aggtgccgct ggaacaggtt
     4861 ctcaaaaccc aggccgacct ctgcacgcat ccgcgcttca aagaagtgct cacggcggtc
     4921 tggcaggatg taaacggcgg ggcgtcgttt gccgacgcgc tggcgcggca tccgaaaatc
     4981 ttcgaacgct tttttgtcaa catggtgcgc ggcggcgagg cctccggcgc gctggagctg
     5041 attatgcgcc gcgtcgcggc cctgctggag cggcgcatgc agctgcgcag taaagtcacc
     5101 ggcgcgctga tttatccttc gctggtgatg gtgcttggtg ttgccgtggt cagcgggatg
     5161 atgctttttg tggttccgaa aataatttcc atggtgggtg agtctggtca gcttttgccg
     5221 gtcagcactc gcgcagtgat tgctgtgagt aatttcaccc gttccagctg gtggcttttc
     5281 ccgctggcgg tcgtcggact gttttatttg tataaaatga agactcgctc cgaagaaggg
     5341 aaggttgctt tcggacgtat gattctgaaa attccattgt ttggaaccct gattgcccag
     5401 gccgaaacca gccgcttctg ccgtatgatg agctcgctgc tggacggcca ggtgccgatc
     5461 ctctccgcgc tctccatcac cggcagtacg ctgggcaacg cggcgctgcg ccagctgatg
     5521 aaggaagtct attcccgcgt gcagggcggc cagccgatgg gccctctgct tcaggcgagt
     5581 ccggagtttc cggagctggc ttcgcgcatg atcaccatgg gcgaagaatc cggcgagctg
     5641 cagaacatgc tgaacaaagt ggcggaccgc tacgaagaga aggtgaccgc caccaccgag
     5701 cggctggtcg gtgtgatcga gccggtatct atcataatca tagggcttat tgtcggattc
     5761 atcgtggttg gtatggtgca ggggatcatg accatgagta cttcaacggg ataggtctga
     5821 aaacagtagg agtgaggaaa taatgaaaaa aagaaaacag gcgaagcagg gctttacgct
     5881 ggttgaactg ttggtcgtga taacgatcct gttgatcatt ggaactttcg cgacgcagca
     5941 ggttatcaat gagccggaca aagccaaagt caaagtaaca aaagcctcct ttacgtcgct
     6001 cgaaaatgca ctagagcggt tcaagctgga tgtcgggcgc tacccgactc aggaagaggg
     6061 gctgtccgcg ctgtacaccg cgcctgacgg cgtcgaaaac tgggccggca aatacctgac
     6121 caagagccgt gcgctgcgcg acgcctggga caatgactac gtgtacatca ttcccggcaa
     6181 aaacaacgag ccgtacgaaa tcatctcgct gggcgcggat aaagccgaag gcggcgagaa
     6241 atacaacgcg gacctttcca atctggatga gaagtaaagg agcgccgaac cgcgatgtgc
     6301 cggaccccga acagcggctt tacgatgatc gaattgctgg tcgtgctggc catcattctg
     6361 gtcatgaccg gcctcgcggt cggcacgctt tccggttccg gtacgcccaa acagcagctg
     6421 cgccgtgagg cgcgcgacct gacggccctg ttgcaggagg cccgcctttc cgcgatggaa
     6481 cgcaaactga aaatcgaggt ctacatcaat ccgcccgccc gcaccgtcta tgccgtcgaa
     6541 tccggttacg cccgcaaact gctggcggcg gacgacggat ttttttcgga gaccgccgcg
     6601 ccggacgggt tcgtgccgga aaccaaccgg tttttccgga cggctgtttt tcctgaaacc
     6661 atcgcgatcg agccgttcgc gctgcaggag attgaaccgg agacgcccgg tacggaaccg
     6721 ctgtttgagg cggcgcccga aggaaccggc gatacggatg ccgcctttct caaccgcccg
     6781 gtattcagct ttacttcgct gggcggcgcc accggcggcg gcatcagcgt tgtgcgcggc
     6841 gaggtgcgga tcgatatcgc ctgcgacctg ctgaccgggc tgccggaaat tgtccggcgg
     6901 agggcgatca aatgaaaaag gccggtttct cactgcttga agtgctggtg gcgctggcga
     6961 tttttgcgat cgccgccggc ggccttctgg tggcgctggg caaccacctg aaaaacgttt
     7021 cgtttatgca ggaccatgcc cgggccgtgc ggatcgccgc ccgcgaaatg aacgcactgc
     7081 ggcggatgac gacttttgcg gaaggcgaaa ccgacggatc ggaggatcgc ttcgtctgga
     7141 cgaagacggt ttctgccgac ggcctcgata cgctgccggg gctgacctcc gcaaccggca
     7201 cgccggcgcg gctttcggtg acggtgaagt ggagcgatcg tccgggcggg ttgccgctcg
     7261 gcaaggtgca gctggacggg ctgactgttt ttggagcggc cgaataatgc gctgtttttc
     7321 catagttcac aggtcggcgg cgcggcgcgg gttcacgctg attgagctgc tggtcgcgct
     7381 ggcgctgctg ggaattctcg gcgcttcgct ggccggggtg ctgcgcaatt ccatggactc
     7441 ggtggagcag gcgcagtcgt ccgtggacca tctgacgcgc ctgcggtcgc tggatacaat
     7501 gctgggcgcg gcgttgcggg acgccacggc gctggcgctt tcgaccaatg agcagaagct
     7561 gctctctgca cagggcagct ttgacgccgc ggcgggcacc atccgctttc acggcgagga
     7621 gcggctgctg gggttctgtt tgcagcgacc gttcttttcc gctgagcgcg acggccacat
     7681 gcactgggtc accctcgaag tgcggcagga cgaaaaaacc ggacgcagtt cgctctggct
     7741 gcaggatatt tcctttattc ccgggctcga caatccggtc ggcgccgact ggggcgggct
     7801 ggaggcggag gtgaaggagc gcctgccggt gcaggaggtt tgtctgatcc gcgaggcggc
     7861 cgcgctgagt tttaaatact ggcttgcgcc ggctgatgcc tccagcgacg ccgaagaagt
     7921 ggaggccgat tcaatcgccg gcgactatgc gctggcggtt cctgattata ttgagctttc
     7981 gattcaattg ccgaagggcg cgccggatgt gctgacgttt gattacagtt tcagccggag
     8041 ggtgctgtaa tgaacgactt cccaggaaat ccgcggtccg gatcggctct gctgatcgtc
     8101 ctgggcgtta tggcgattct gtcgctgctg gtggccggct tcggccgcgg actgcgcgcc
     8161 gatctgcgcg cctccggcgg gttctatgaa gccgagcaga accgccagct ggcgctcagc
     8221 gcgctggcgg cgtcgcaggt ggagcgcgcg cagacgctgc tctatgccga cgcgaccggc
     8281 aatgtctatt ttgtgagcag cgatgacacc gccgagtcgg aaatcgcggc gctgtcggcc
     8341 tatcgtcagg ggctgccgct gggccgcgga atgctgtcgt accgtttcgt catgaagccc
     8401 gccgcgctcg atctgaacag tctggacgcc gccgcgctgg gccgcctgtt cgaggtcgcc
     8461 tgcggcatgc cggaaagcga cgagcgctcg gcgctggccg atgccatact ggactgggca
     8521 gatgccgaca gtctgccgcg ggcctacggc gcggaggaag aggtgtatca ggcgctggtt
     8581 ccgccccggc actgccgcaa cgcgccgttt gaaacgctcg aagaactgct gctggtcaac
     8641 ggcatcacgc cggacctgct gttcgggtac agccacccgg tttccattga gaacggactg
     8701 ctgctcggcg gcgggctcta tcggttcctg atcggcgaca acagccccga ggcgcaggct
     8761 tcggtgcagt acattctgcg cggcgtgctg cccgacgatg cgaaagcggc ggagccgccg
     8821 gctgaagggt ttaccaaagt gaccgtgctg ccgccggtgc tttatctggt ggcggaaggc
     8881 tttatgccgg ctgcgccggc ggaaacgaaa accgcatcaa ccggcgaaca gccggtcgaa
     8941 gcggcttccc ggcataccat tctggtgaag ctgatattgc cggacacggc gcaggcgaaa
     9001 acttatacgg ttgctgattt tcaggaaaat gccgccggcg aactgctcgc gcaggtgctg
     9061 gcgtatggaa tcccggagga gacaaatgaa ctttaaaaaa atgccggaga tgaagtggat
     9121 cgccggaaac tcgcgcctgc tgctggcgtt tcaggacagc accgtggacg cggtctggct
     9181 ggagcagtcg ctgctggggc cgtcggtgcg gatgatggag cggttcccgc gcgatgagca
     9241 gctgcccggt cggctggtgc agcggctgca ggccgccgga aaaacgccgg cccgtgtaac
     9301 ggtctgtctg ccccggtcga tggtgatgca gcggacgctg gcctatccgc tcgccgtaca
     9361 agacgatctg gcgcagatgg tgcagttcga ggcatcccgg cacgttccgc tgccggaagc
     9421 cgaccgccgg atcgcgtttg ccgccgcgcc gctgccgggc ggtaaacagc tgggcgtgaa
     9481 tctgctggcg gcccgcacct cggaaatcag cgcgctgatt gatccgctgg tcgcggcagg
     9541 gctgccggtg gatgaggtga ccgggctgag ttcgctgctg gttccggccg acccccgcct
     9601 ggcggtgctg ctgattctga cggacgacac ccagctggag ctggcgctgt tcgccggcgg
     9661 tctgctgcag gaccgtctgc tgctgaaccg gcaggcggcc ggtttcggca aggagacgct
     9721 ggtggatgcg gtgcgccgca tgaccgtgcg gcaccacgcg ctgctgggcg ctgaaggcat
     9781 cgggcgggtg gtctgcggcg gtgtgtcggc gctgccggat gaatttcagc acagcctcga
     9841 agcgaccttc ggcctgcata tccatgcgct ggaggttcct ccggcgttgc agtccgcgct
     9901 gccgccgggc gcgccgctgc tggcggccgc gctggccgcc gcggtcagcg aattcgcgcc
     9961 ctcgttaaat ctgattgaac ggtccggccg caaggtgtcg ctgagccgcc ggacgctgat
    10021 cgttgccggg ctgtgtgcgc tgctggccat cgaactgctg gccggcgggc tgctgcggac
    10081 gtttgcgccg gcggttgcac tcaaggcggc cgagcgcgag gcggcggagc tgaaacggcg
    10141 tacagcgccg attcaggcgg tgaagagcag taaccgggcg ctgcgcagtg agctcgaaca
    10201 gctcacggat ctgggtaaaa cccgcgtgag cctgatgcag atgctgaagg tgctttccga
    10261 tacgctgccg gaagatacct atctggagtc gatttcctat acccgcggcg acgacatcaa
    10321 gctgaagggc cgctccaaaa cgccggaccg cctgccgcag ctgattcaaa gcctgccgtt
    10381 tgtgaaaacg ctggaggcgt cggatatcgg cgagaagaac gacgactatt tcggattcac
    10441 tatttcagcc gcgttaagga gtgtgcgtga tgaataattt ctttacccga ttgtcccggc
    10501 gcgagcggct ttatgtgctg ctggcggccg tcatcctgct ggccggcgga attttctatc
    10561 cgctcttccg ggcggcgcag gcctaccggc aggccaagct cgaagagctg gagtccgccc
    10621 gttcgctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntat ccggaaatcc
    10741 tgaatctggt ttatcagctg gaaatccatc gcccgctgat tgtggtcgac cggttcagcc
    10801 tgtcggtgca gagcggccgg cgatccttcg ggcagaataa ccgtccgtcg ggcggaccga
    10861 gcggttcgac ggggttttcc ggatccggcc ggtcgggctt cttttcgagc cagttttcgc
    10921 ggcggtccac ggcggccgat acgccctccg aaccccggat gcgcctgcaa atggatattc
    10981 acatcaacgt ccggccttcc gagggaggtg cgaaatgaaa atgattgccc cccgctacca
    11041 gattccgctg ctgatcggac tgtgcgctct tgccgcgctg gcgttgattc cggcggtgca
    11101 tattccttca cctgcggcgg cttcgcgggc cggcgccgcc gcaccggttc cgaccgggcc
    11161 ggtgacgttt aatgatacgc cgccgacgat gcacgaagtg gccgccaaac atctgtttat
    11221 tgcccagcgc aaagccaccg gacagaaatc gtttcccgat ctggtcgtga aaggcatttt
    11281 tatcggcgcg gaaaaaagcg tcgtgctgtc gctgaaatcc aggccgcagg ccaatctgcg
    11341 ggtttgggag gacggcagcg accgggctct gagccagatt acgaagccgg acgatccccg
    11401 ccagccgctg gccgcatttt tgcgcgagtg gaaggtgaag gaaatttcat tcaacggtat
    11461 ttcggtggaa caccagatca ccggcgaggt ggagacctat gcggtggact atacgccgga
    11521 gaagaaggtc aaggatgatg cctcccgcgg ttacgggcag gggattattc cgcagggagg
    11581 tgacggcgga ccgtccgaca gtgtgcggac tgccggaagt aatacctccc ggccggtcca
    11641 gccgcagcag caggttactg caccgccggt tactcagatg gccgaccgga tttccgagat
    11701 ggttcagcgg atgaccccgg atcagagaaa acagttcctg cagcgggttc agcagaacat
    11761 gaccggaggg aatcagaata acagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnactgtc cggcctgcgc ggggcggtaa tccgtcaaaa gtgccggatt cggagtttaa
    11941 aacatacgga ggacgacgtg agggatagca aaggatcttc ttcgaaaagc gggtacaacc
    12001 ggtttctgtt gctggttgcg ggtctcggcg gcctgttgta cggcattgat gtgggcatca
    12061 tctccggcgc tctgccgtat ctcgaggcga cgtttaaaga ttccgcgacc ggccagtcgc
    12121 tgacggccgg aaacctttcc ttcattgtgg cggctgtgct gctcggcagt gtgatttcga
    12181 cgctgtttgc cggggtgctg tcggactgga tgggccgcaa aaaactgatg tttctgagcg
    12241 gcctgctgtt tgtcgtcagc ataccggtca tcgcgctttc cagcggctac agctcgctgg
    12301 tgctggggcg tctgctgcag ggcgtcagcg cggggctgat cggcgtggtg gttccgctct
    12361 atctggcgga atgcctcggc gcggccaacc gcggcaaagg caccggagtg tttcagtggc
    12421 tgctgacgct cggcatcgtg gcggcggcgg gcatcggcta ttacttcagt caggaggtcg
    12481 cgcagattga aaaactgcag gatgcggcca agctgtttgc cttcaaagac accgcctggc
    12541 gcagtatttt ctgggtttcg ctcccgccgg gactgctttt cgtgatcggc agcctgctgg
    12601 tggcggagtc gccccgctgg cttttccgta aagggcgcaa agaggcggcg ctcgcggccc
    12661 tgctgcggtc gcgtaccgat gaacaggctc acaacgagct ccgcgaaatg gaagaaaccg
    12721 ccgccgccga aaaagcgcag accaattcgg cgggcgcgaa aatcaaagaa tcgctgctgc
    12781 gccgcaagta cgtcattccg ttcgttctcg cctgcgtcat tctcgcctgc aatcaggcga
    12841 ccggcgtcaa ctccatcatc ggctacaatg ccacgattct gattcaggcc ggactcagcg
    12901 atgccaatgc ccatctcggg tatgtcatcc tcacgctggt gaacttcctg gtcacgatgg
    12961 cgggcgtggc gctggtggat aaaaaaggcc gcaaattcct gctctcgctg ggcagcagcg
    13021 gcatcatcat ctcgctcatt ttcgccggtc tggttttcca taagaacgaa aaagcgcggg
    13081 tggacgtgaa ggaagccgtg caggccatgg tgatcgagtc gaaccagacg gcctccatcg
    13141 acttcaacaa cgaggtcgcc gcgaagctga tggcggataa gggcggggcg gcgaaccgcc
    13201 agtcagccac aatggtggtc atctattcct acggcgattt ccatgccgcg tcgaaggtcg
    13261 tccgctccga cgacatcgcc gccaagatcg aaatcagccg cgccagctgc gtgccggtga
    13321 acaaagtggc ggcctttttc cagaacccct ttgccgatct cgacgcggcg cgggtggctc
    13381 cgctgaagat cgataacgcc ttgattacgc cggtgccgtc ggagaaaagc ggctggctga
    13441 ccgccatttg catattctgc ttcatggcct ttttcgccat cggcccgggc gtttgcgtat
    13501 ggctggcact ttccgaactg atgccgacgc gcatccgctc caacgggatg agcatcgcgc
    13561 tgctcgtcaa ccaggcggtc tcgacctcta ttgcggcggt ctttctgccg acggtcggca
    13621 aatacggata cggcaccatg ttcttcgttt ttgccggctg cacggtggtc tacttcatca
    13681 cggcgacctt cttcctgccc gaaaccaagg gcaagacgct ggaggaaatc gaagagcact
    13741 tcgaaggcgg aaaataaggt ttcgcgcagc ccgaacggcc cggcgagccg cgtaactgtt
    13801 tagtgtttaa ggagaaacgt atgaagaaac gatccattta caaaatactg gcaactcttc
    13861 tgatgagcgg cgcagtattc gccgcgccca ttgatccggc tatcgacgat gcgccgggcc
    13921 cgttttgcta cttcagccgt ccgagttttt attttacagt cgcggaccat gacgacggta
    13981 cacaggttac tccggagggc tggctgtgga ccggttcggc gcagatgctg tttttcgccg
    14041 gcgcggatct tgcaccgttg cgggagcgca ttcagacctt gcgtgaaccc ggtgtgccgg
    14101 ttgtcgtgca aacgtcccgg atcggagata tcgtttatga aatcaccaga ttcggagcga
    14161 ctttggatgg taacccgcag agcgctctgg tgaatttcat tcgtgtgcgg atgaaaaatg
    14221 aaggcaaaca gcccggtgaa gcaacctttg ctgttgccgt acgcggtaag ggagatcatt
    14281 gctgcgagcg cctgaccact gcatggaatc cggttgcggc tcagtatgag atgggtgcca
    14341 actgcgccgt ccgcgacgga aatcttttat atatgtttcc ggttgatccg ccgccaaaac
    14401 gttttgtggt gccggacgcc gagaattccg gtccggtcaa ggcgctggat gagtatgtcg
    14461 gtctcgccga accggtttgc atggttcgct atggtctgga tcttaagccc ggtgaggaaa
    14521 aggccctcga atttaaaatg cctttcaagc cggtatcggt gaaggatcaa aagacggttg
    14581 atgcactggc gggtgcgagc tttgataaat atctcaagca gaccgaaaaa tggtggcatg
    14641 aattccatgc gaacggaatg cagatcgagc tgcccgaaac aaaagtaacg gatacgtttc
    14701 aagccagtat catgttcgac accatggccc gggaaaaaat cggcgacgac tacatcgtga
    14761 ccgtcaccca tttgaactat cacgactact atctgcggga cggcgcctac atcacgcacg
    14821 ctttggatct caccggacgg aggcagtggg tggagcaggg gcttgagtat atgctgaagc
    14881 ggcacgagcc tgacggcagg attgtcgaga aaccggcgtg ggacggctat ggtcaggcaa
    14941 tgtgggcgtt cggcgcgcat tggaggttga ccggcgacaa cgaatgggcc cgcaacgtgt
    15001 acccgaatct cgctcggcat gtgcgtgccg tgtttgccca agttgcaacg gatccgctgg
    15061 ggctggtgcc ggttgcgccg ccgtatgata acgaggggat caacgggcac tacaccggcc
    15121 acagcttttg gatgctgatc ggaatggcgg acatgattgc gatggctgag gcgctcggcg
    15181 aaaaggccga tgccgagcaa ttcaagcaat ggcgcgacga ctataaagcc aacttcacca
    15241 aagcccttga cgccgccacg gccaaaaccg gcggctatat tccgccgggc cttgatgcgg
    15301 ataacggcaa tgactgggac aacctgatct cgctctatcc gcagggcgga gtgccggcca
    15361 aaggcgcgct tgatccggca gacccgcgca tcggcaagac acttgatatg gtgcggaaga
    15421 accagtaccg ggaaggaatt atgacttacg gccgcggatt gaaagccggg gcgttgcatc
    15481 actatctcac tttcaaagcg acggaaggac tggttgcctt gaaccggcaa cgtgaggcgc
    15541 ttgaagattt gtacggtgtt ctggcgcata ccagctcaac cggtgccggg ttcgagttta
    15601 aacccggcgc agacggcgtg tggggcaacc gtgatacgga aggcaattat ccgtgtcacg
    15661 gctggtttgc agcggaatat atcggactgt tgcgcaatct gctggtccgc gagtccgccg
    15721 gagatctgca cctgttcagt gtgctctctc cggaatgggt gaaacccggc agccgtattg
    15781 cggtgcgtaa tgcgccgacc gatttcggta tgatttcggt tgttgccgaa atcaaagacc
    15841 gtgcgatgac ggtcaatctg gatacccaat ggcggacgcc gccgcgcagt ctcatcatgc
    15901 atttgccgtg gtacttggac ggcgctgcgg ccaccgttga cggtaagcct gctgtgatca
    15961 gcaagccgcc gtacggtgaa ggccagcaga ttgtcctgcc gccgaaagcg cgaacggtca
    16021 cggtgcagtg gaagccggcc ggtttgccgg agttgagttt tgcaaatacc gtggctcaat
    16081 ggaagaagca gaaccggcag cattatgatg agtatgtggc tcagggcggc aagccggaac
    16141 tgttgtggaa agaaccggcc ttggcgatga cccgtgccga acggcagaag ggctggattg
    16201 ctgaaaaagg tattgccgtc ggctgcaaga cggcggcaag cggttttgaa gaaggacatc
    16261 aaccggagaa tgccgccgac gggcgcgtgc atcgcccgac cgagtggagc gtaaagacgg
    16321 ctccggcctg gtggcaggtg gaccttggta aagcgcagct gatcgattcc gtgcgggtta
    16381 tatccgcttg ggagccaaac aaagagaaac tctcatggca atataaggtg ctcgtttcga
    16441 cagacggcag ccagtggcag actgtttcgg attacagcaa caaccagaaa accgaaactt
    16501 ccaaggggac gttgcacacg ttccagccgg tatccgcccg ttacgttcgt gtcgaaatgc
    16561 tggggaattc aaagaaaatt cccgcatcga gccttaatga agtgaaagtt tttccggcca
    16621 tgccgccagc ggcggaattt tagaccggac ctgaccgggt cggtcatcgg cctggcagga
    16681 tttatcgccg atacggcaga tccaagccgt atcggaaatt tctgggcgaa ggcggcaaat
    16741 agggtttcgc gcagcccgaa cggcccggca aaccgccggg ccgttttgcg ttaaagcctc
    16801 ccttgaggta attttttcgt gtattcaccg gaggctctgg gctattcttg cccctcaatt
    16861 tttgaggaat tttcgatgaa atacatcagt accagagggc agatggaacc ggtcgggttt
    16921 ctggatgccg taatgaccgg actggccccc gatggcggcc tgctgctgcc cgaaagcctg
    16981 ccggacgttt cccgtaaact tccggtctgg gccggacttt cgtaccagga gctggcgttt
    17041 gaggtaatga gtctgttcgc gaccgatctg ccgcccgccg acctcaaggt tctgattgaa
    17101 aaaagctatt ccactttccg cgcccctgaa gttgcgccgg tgattcccgt cgacggattc
    17161 catgtgctgg aactctggca cggcccgacg ctcgccttca aagatgtggc gctccagttc
    17221 ctcggcaacc tgttcgagta cgttctcgaa aaacgcggcc cttcggccag ctcagggcag
    17281 gcggccgaac tgaatattct cggtgcgacc agcggcgaca ccggctccgc cgccatctat
    17341 ggcgtgcacg gaaaaaagaa catccgcatt ttcatcatgc acccgaaagg gcgcaccagt
    17401 ccggtgcagg aaaagcagat gaccagcgtg ctggacgata acgtcttcaa cctcgcggtc
    17461 gaaggcacgt tcgacgactg ccagcacctc atgaaatcga tcttctccga cgtggagttc
    17521 aaagagaaac actcgctcgg ctccgtcaac tccgtcaact gggcgcgggt gctggcccag
    17581 atcgtttact acttctacgc cgccttccgc gtgcaggaaa ataccggagc cgcgcaggtg
    17641 cagttcgcgg tgccgaccgg caacttcggc gacatcatgg ccggctatat cgccgtacag
    17701 atggggctgc cggttaaacg gctgattctg gccaccaacg aaaacaacat cctgtcgcgc
    17761 ttcttcaaca ccggcgtcta cagcatcggc gaagtcgtgc cgaccatcag cccgtcaatg
    17821 gacattcagg tcgccagcaa cttcgagcgc tacctctatt accgcgtcgg gcaggacgcc
    17881 gccaaactgc gcgacctgat ggccggcttt gccaaaacga aaaccctgac ggttcccggc
    17941 accgatgaat tgttcgttgc cggcgaagcc ggtacggcgc agacgctggc gatgatcaag
    18001 aaatgttacg aaaaggaagg ctacattctg gatccgcaca ccgccgtcgg cgtggcggtc
    18061 gccgagcgct ttgcggattc cgccgtgccg accatctgcc tggcaaccgc gcatccggct
    18121 aaatttaatg atgcgattca gcaggcgatc gggatcgccg cgcaccatcc ggcgctcgac
    18181 aaactcgcca acgccgaaac ccgctgcgac actatcgcca acgacgaggc cgccgtgcgc
    18241 gattatctga ttgcaaaaac gaagtgagga atgccgctat ggctgacaaa aagaacatga
    18301 gaatcctttc gggtatacag ccttcgggca aactgcacat cggcaactat ttcgggatga
    18361 tgaaaccggc catcgaactg caggagcagg gcgaggcgtt tctgtttatc gccgactacc
    18421 acgcgctgac gacggtgaac gatgccgccg cgctgcgcac gatggtgaaa gatgtcgcgc
    18481 tcgattttct ggcgaccgga ctcgacccgg aaaagacggc gttctaccgt cagagcgatg
    18541 tgccggaagt gcaggagctg gcatggcttc tttcggttgt aacgccgatg ggtctgctgg
    18601 agcgctgcca ttcctacaag gacaagctcg ccaaagggat tgccgcgacc cacagcctgt
    18661 ttgcttatcc ggtgctgatg gccgccgata ttctctccgt gcagtccacc gtggtgccgg
    18721 tcggccgcga ccagaagcag cacgtcgaag tgacgcgcga tatcgcggtg aagttcaata
    18781 accagttcgg cgaggtattc accattccgg agccgtcgat tcgcgactcg gttgcggtgg
    18841 tgccggggat cgacggtcag aagatgtcga agtcctacgg caacacgctg gaaattttcg
    18901 gcgatcccgc cgcgctgaaa aaacgggtga tgcagattgt gaccgacagc acgccgctgg
    18961 aagaaccgaa gaatcccgac acctgcaacg ctttcaaact ctatcagctg tttgcttcgg
    19021 atgcggaagc tgccgacctc gccgcgcggt atcgcgccgg cggcttcggc tacggtaccg
    19081 cgaagaaaga gctgcttgcc aaaatcaatg aacattttgc gccgatgcgc gaaaagcgcg
    19141 ccgaactcga aaagaatcct gacttcgtaa acgatgtttt gaaaaagggt gcggaacggg
    19201 cgcgcgccga aacccgtaaa acccttcagg ccgcccgcaa agcggttggg ctggaataat
    19261 gcatatgcct gatttacttc cgaatgaaga atacaaagtg agtctcgaag ttttcgaagg
    19321 cccgctcgat ctgctcctct acctcatcaa aaaagacgag gtggacatct acgatattcc
    19381 gatcgggcgg atcaccaccc agtacatgga atatctcgag ctgatgaaaa tcctcaatct
    19441 cgagatcgcc ggcgacttca tcgtcatggc ggcaacgctg atggtgatca agagccgcct
    19501 gctgctgccc gacgaagcgc gcgggccggt ggaagaggac gacgaggatg atccgcgctg
    19561 ggatctggtg cgccagctgg tcgaatataa aaagtttaaa gacgccgccg gatttctcga
    19621 acatctcgaa gcggtgcagg agaatgtttt tctgcgcgaa ggtgagcacg tcgaactggg
    19681 cgcacggccg gagatcggca tgcaggacgt cagtattttc gacctgatca gcgcgctcaa
    19741 tcaggcgctc gaccgcgtgc cgaaagaaga gctgaacgag attttcgctg aacagtacac
    19801 cgtcagcgag aaagtggact atctgctgga agccacgaag aacggaaagc cgctgacgct
    19861 gggccgcctg ttccgcggga tgcgctcgcg ccatgaaatt gtctgtacct ttctggccgt
    19921 gctggagctg atcaaactca accgtctggc cgcgcggcag gacaaccatt ttgaagaaat
    19981 cgttattgaa caggtcgcgg gcggcgagcc cgaaccggtt ttgcaggagg aagagaccgt
    20041 atgagtgatc tggaagtact tcctgagctg aaggaaatta tcggcgcgct gctgttcgca
    20101 gccaagaagc cgctgacggc gaaagaaatc cgcaaagcgc tgctggatgc cggcgccagt
    20161 tacggcggcc cgtatcagga gttttcggaa tttaaagatg acgacatcct ggccgccatc
    20221 gaggatttga aaaccggtct ggttcagagc ggatcggggc tgtgtattgc cgaggtggcg
    20281 cacggtttcc ggctgcagaa caaaatcagc tgcggcccgt gggtgcgcac actgctggat
    20341 aaagaccgga cggcgcgtct gtccaaaccc gcgctcgaaa cgctcgcgat cattgcctac
    20401 cgccagccga ttctgcgttc cgagattgaa tccgtccgcg gcgtggcggt cgatcaggtg
    20461 ctgcgcaatc tggtggaaat gcagctcgtc aaggtgacgg gccgcagcga gctgccgggc
    20521 cgtccttggc tcttcggcac cacgcagcgt tttcttgagc atttcggact gcggagtctc
    20581 gaagagatgc ctggcatgga agaactgaag cggatggttc cggtggagaa agcggaacgg
    20641 acaaaacctt tccctgaaat ggaaaaagag ttagaacagc gcgaggaaca ggcggtaacg
    20701 gaggaaagcg atgagtctgg agaagctgag gaagcagatt gatggtctcg atgaagagct
    20761 gattaagctc ctcaacgagc ggatcaaaat cgcgctcgaa atcgggaaaa ccaaaaaaga
    20821 gcagggcgga gaaatttatg ttccctcccg cgaaaaagcc gtctttgaac gcattgcccg
    20881 gctcaacgcc ggtccgcttc cggaagaatc ggcgcgcgcc atctaccgcg aaatcatgtc
    20941 ggcggcgctg gcgcttgaga gcaacctgaa gattgcctat ctcggtccgc cggccacgtt
    21001 cacccaccag gccgcgcacg ccaagttcgg tgccagcgtg gattatcatc ccgtcggcac
    21061 cattaccgac gttttcaacg ccgtggagca cggttcggct gactacggcg ttgtgccgat
    21121 cgagaactcc atcgaaggtg cggtaacgca caccttcgat cagttcaccg gaacgacgct
    21181 caaaatctgc gcggaaattt atctgccggt atcgctttat ctgctcgcct ccgtgccgaa
    21241 agaaaacatc acacgggtct gcagcaagca ggaagcgatc ggacagtgcc gccgctggct
    21301 cagcgaaaac ctttccggcg ttgagcttga tgcggtcaac agcaccgcgc tggctgctga
    21361 aatggcctca accggcgatt gcgccgccat tgccagcctg ctggctgccg aactgtacaa
    21421 tctcaatatc attgccgaaa acattcagga ttccagcggc aatacgaccc gcttcctcgt
    21481 catcggccgc aaatacggcg agccgaccgg cgaagacaaa acgtcgatct acttcggcat
    21541 caaagacgaa gtcggcgctt tgcatgacgc gctggatgcg ctcaagctca acggtatcaa
    21601 tatgaccaag atcgagtcgc ggcccagccg gagcaaggcg tggcagtact acttcttcgt
    21661 ggacttcgaa ggtcatgcgg acgatccgca cgtgaagacg gcgctcaagg cgctcgagaa
    21721 acactgcgcg gttctcacgg tgctcggctc ctatccgaag gcggacgatt gaatttccag
    21781 gatcggaacg gcatgagtga catactgaaa atttcggtcg gagttgtggc gtggaacgag
    21841 gaatccagca tcgcgctggc gctggaatcc atccttaaac aatcgatctt ccgcaatgcg
    21901 aggagcctcg atctggagct ggagctgatc gttatcgcca acggctgtac cgaccggacg
    21961 gttgaggtga cggatgattt tttcgccaag gcgcgcagcg agtttcctga aggcagcgcc
    22021 gtcagcctgc ggcttgagat tgtgccggaa gccggtttcg ccaacgcctg gaatctgctg
    22081 gcgcacacgt acagccgtcc cgacgcggac ttcatctatt tcgtcaacgc cgacattgtc
    22141 atcgaccagc ccgatgcgtt tgagcagatg ctgcgcggcc tgcagcggga ctacgccatg
    22201 cgggtcatct ctccggtcgg gcacaaacac attgaggcgg cgaaatgcaa aacgctgtgc
    22261 gatcggctga atctggccgt tacccgtttt accgagaaga accagacggc gcaggttcgc
    22321 gggcactgtt tctgcgcgcg tgccgccgcg atgcgcatgg tctggcttcc caaatatttt
    22381 cccggcgcgg tggacggctg ctttaagcgg atgatcgtca ccgacatggc atccgcggaa
    22441 ggccatccgg cgcgcatcgg cattgcgcgg aatgtcagct atacctttga agcttatcgt
    22501 acgttccgtg aaatttattt ccgtcgggtt cgtcttttca tcggccagac gtttatcgaa
    22561 acgcttatgc gtcatctcaa aacgatgcgc gacgaacagc cgaaactgaa tccggcagcc
    22621 tatctgcggg cccgcgacga gaccgatccg tcgtggctgc accgtctcgt acagctgaat
    22681 atccatgtaa acgggctgtt cgagacgatg ccgtcgccgt ggttccgttt ccgcttctgg
    22741 aagaatccgg gactcagccg gtttaaaaaa ctgggcatgc ttcctgttgc ggttaccgcg
    22801 tttgtttttg atgtgcccgt ctttctttcc gccgcgtggc gtctgaaaac cggccgcgtg
    22861 gccgatgtct ggaaagatac gcgcaaccgg gaattgcgga cataaccgta ctgcgaggag
    22921 atgcctgtgc cgtcccatac gctccaattc cgtgtccgct acagtgaaac cgatcagatg
    22981 cagacgtttt acaattcccg tgcactggaa tggttcgaag tcggccgcac cgagctgtcg
    23041 cgcgccgtcg gcgtgccgta ctcggagtgg gagcggcggg gccttctcat gccgctggtc
    23101 gaggcgcggg ttttcttccg cggacgggcc ggttatgatg atctgctcga aatgcgcgtc
    23161 accctcaagg ccgaaggccg cgcgcggctg ctttttgaaa accatattgt gcaggccggc
    23221 accggcgcgc cggtggctga cggctatacg gttcatgcga tggtttcact ggaaaccgga
    23281 cgcccggtcc gcgcgccgga ctgggtggtt gagcttttga ataaggaata aaaaatggaa
    23341 cgacagtttt tgatgggaaa tgaagcggtt gcactgggcg ctctgcatgc gggcgccgcc
    23401 cgcggggccg gctatcccgg aactccttca agcgaaatac tcgatgtctt caatcagctc
    23461 ggcggcaaag ccgaatgggc gccgaacgaa aaggttgccg ccgaggtggc gctgggcgtt
    23521 tcgtttgccg gcgtacgcgc ttttaccacc atgaaacatg tcggactgaa tgttgccgcc
    23581 gatgtgcttt ttactgccgc ctacagcggc gtggacggcg cgctggtttt tattgttgcc
    23641 gatgatccgg ggctggcttc cagccagaac gagcaggata cccgccggta tgccgtcgcc
    23701 gccggcgtgc cgctggttga gccgtccgat tcgcaggaag cctacgactt cacccgtctg
    23761 gcatttgaaa tttccgggcg ctggaaaatt ccggtgatcg tcaagctgac cacccgcgtc
    23821 tgccattccg gaacaattgt cgtgccgcgc aaaccggacg ccggtctgcc ggaggcgcac
    23881 tttgagcgca acattccgta ccatgtcatg gtgcccggtc acgcccgccc ggcacaccgc
    23941 cgtctgcggc agaagctggc ggaaattgcc gagtggaacg aaaaggaagg tccgaatcag
    24001 aaagtcaaag gatcaaagga tctcggcatt ctggtttcag gcgttgccta tcagcacgca
    24061 cgggaagccg cgccgggcgc ctctttcttt aaagtcggaa tgagttatcc gctgccgatg
    24121 cggcagatgc tcgacttcgc ggcgtcggtt gaccgcgcat ttgtcgttga ggaaaacgat
    24181 ccgtatatgg ccgatgctgt gcgcgccgca ggcggcacga ttgaaagcgc gccggaaatg
    24241 taccgcttcg gcgagctgga tgtcggccgc gtgaaacgta ttctgactca ggatgcaacg
    24301 cccgaagcgg ttccgccgcg cggtaaaccg ccgcagcttt gcgcgggctg tccgcataca
    24361 attacctttg agatgctgaa aaagcataac tgcatcgttg cgggcgacat cggctgctac
    24421 acgctggccg cgcttcagcc gctggcggcg atggacacgc agatctgcat gggcgcgagc
    24481 ctcggtgtcg gcttgggact tcgccacgtt cttccgcgcg aagaagccaa gcgtgttgtc
    24541 agtgtcatcg gcgacagtac gtttgttcac agcgggctga ccggtctggc ggaaatgacc
    24601 tataacgtac cgccgaccgg ccatgtgctg ctgattcttg ataacgccac caccgcgatg
    24661 accggtcagc aggaacaccc cggcaccggg cgtctgctgg accatacgcc ggcgaaccgg
    24721 ctcgactatg aaaaaattgt ccgcgccatg aatgttcaga atgtcggagt ttttgatccg
    24781 gtcacacagc gcgacgggta tgaagccgca ttgacgcaag ctcttgaaaa agacgaactg
    24841 acagttctga ttgttcggcg gccctgcatt ctggcggtgg cccgcaaagg tgttaaacct
    24901 gcaactcagg aataatttgt atgagcgata aagtaaccag tattaaaatt gccgggctcg
    24961 gcggtatggg cgtgctgacg tcaacacaga ttctggccga ggttttcttc cggcagggcc
    25021 ttgatgttaa aaaagcggaa gtgcacggca tgagccagcg cggcggctcc atctgcagcg
    25081 atgtgcgctt cggaaaaaaa gtgttcagtc cgatggttcc ggcgggctcg ctggattttc
    25141 tggtgctttt tcaggaagac cagtttcctc tgtatgaggc ggactgtgcg cccggcacct
    25201 tgattttaaa accggcggcg attgatctgg caaagctgga gaataaaaaa gcgctcaaca
    25261 tcgccatgct cggcctgctg agccggcacc tggatgtccc ggccgatgtc tggctggaag
    25321 tcattcgtga ggttttgcct gcgaaactgc ataaagtgaa cgaggcggct tttgagttag
    25381 gaagaaaaag tgtgtgagtg tgtgcgggcg tgagtttgtg aggaaatcga aatgggattg
    25441 gtgaaacatt tcagagattt gcgggtgtat cggacagctt tcgatgccgc tatgcagatt
    25501 tttgaactgt caaaaaaatg gccttcagag gagcggtatt ctttgaccga ccaaatccgc
    25561 cgttcatcgc gctctgtttg tgaacagatt gctgaagcct ggcgcaaacg ccgttatcct
    25621 gcacatttcc gcagtaaatt aacggatgcc gatagcgaag cggccgaaac tcagtcgtgg
    25681 cttgaatttg ctctgcggtg cggttacatc acgaaggatc agtttgtctc attggatgcg
    25741 gtgtatgagt ccattaatgg cggactggtt cgaatgattg atcaatcgga acagtggtgt
    25801 gtctttcagc cgaaggtgga agaagagggt tgtgactatt tgctttctga acaactcaca
    25861 cactcagata ctcccgcact cacaaacaca cccgacggca gcagagctga agatgatttt
    25921 actgacgaat aaggtttagc ggagcgagaa aacacatgga acggattatt catcctgaaa
    25981 gtatcccgga ttacatggag tccggcgagt tgcgggcttt gcagttgcag nnnnnnnnnn
    26041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn accggcggcc tcggcgctca ttacggcgcg
    26161 gaacggctcg gcgcgaccgt cattccgatt tccggcggca atactgagcg ccagattctg
    26221 gtgatgaagg attttggcgt gacggcgatc tgctgtacgc ccagctattt cattcatctg
    26281 atcgagaaga taaatgccag cggtatttcc ttcagggagc taggcgtaaa aatcggcgtg
    26341 ttcggcgcgg aaccgtggac ggaagcgatg cgcacctaca tccagcgcga atccggcatc
    26401 aaggcctttg atatttacgg gctgacggaa atcatcgggc ccggcgtcgg tatcgaatgc
    26461 tgcgagcagg ccggactgca tattttcgaa gatcattttc tgatggagat tgtcgatccg
    26521 gaaaccgatg aagtccttcc ggacgggcag gagggcgagc tgattatcac aacactcact
    26581 aaacaggcgt ttccgatgat ccgctaccgc acacacgata tcacatcgat caatccggag
    26641 cgctgcgcct gcggccgcac gatccgccgt atcaaccgca tccgttcgcg cagtgacgac
    26701 atgatgatca ttcgcggcgt caacgtcttc ccgtcgcaga ttgaagccgc tctgctgtcg
    26761 gttgaagaaa ccgtgccgca ttaccagatt ctgctgaccc gcaagggcgg gctcgatcag
    26821 atcgaagtgc aggtcgaagt caccaaagaa atgttcggcg accgcatcag cgaactggaa
    26881 tccatgcaga tgcgtctgat gcatgcgatt gatcatatta ccggactgcg cgtgccggtc
    26941 cgtcttgtcg agccgcacac catcgcgcgc agcgaaggga aagcaaagcg gatcgtcgat
    27001 aaacgcagcg aaggggagga gatacgatga aaatcaaaca gttatccgtg tttcttgaaa
    27061 accgtcccgg ccatctcagc cgcgtctgtg aagttctggc ggaagccgga ctcaacatcg
    27121 tcaccatgac gctggcggat acgctggagt tcggtattct ccggctgatt atccgcgaat
    27181 ggagccgcgc caaaacggtg ctggaagccg ccgggtacgt tgtgaacgtc accgacgtaa
    27241 tggccatcga agttaccgac gaacccggcg ggttggagaa aattctaaag acggcggaaa
    27301 ccggcggact gagcgttgaa tatatgtatg ccttcgcctg caagcagaat aaaaacgcgc
    27361 tgatcgtcat tcgttttgat aatccggaac gagccgccgc cgtactggcc gaagccggaa
    27421 tcgggatttt gtccgccgca gaattttatg cttaaggaga accgtatgat ttcaaataaa
    27481 tggattgaaa cgctgccggt ttacgagccg ggtaaaccga ttgaagaagt tgcgcgcgaa
    27541 ctcggtttcg ccgacatttc ggaaatcatc aaagtcgctt ccaacgaaaa tgaactcggc
    27601 ccgtcgccga aagcgatgaa agcgatggcc gccgccgcga cggaaatgca ccgctatccc
    27661 gacggcggct gcttctacct caaagaaaaa ctggccggcc agctcggcgt caaaccgcag
    27721 aacctgctct tcggcaacgg cagcaacgag ctgattgaat ttcttggcca tgtttatctg
    27781 ggtgccggaa ccaatctcgt catgagcgaa ggcgcgtttg tcgtctaccg gcttgtcgcc
    27841 atgctgttca acgccaaggt catcgccgcg ccgatgaaaa agttcggcca cgacctcgac
    27901 gcgatgctgg cggccgttac gccggaaacg cggatggtca tcatctgcaa cccgaacaac
    27961 ccgaccggta ccatcgtcac gcccgcaaaa atcaaagcct tcctcaaaaa gctgcccgcg
    28021 aacgtgctgg ccgttttcga cgaagcctat ttcgaattca tgcccgacga tcaaaagccc
    28081 gatctgatca aagaaattca aaagggccgg gagaatgtca ttgtcctgcg caccttctcc
    28141 aaagcctacg gactggccgg actgcgcatc ggctacgccg tcggacacga aaaactcatc
    28201 gcactgctca accgcgtccg ccagccgttc aacgtcaact ccatggcgct ggccgccgcc
    28261 ttcgccgcgt tcgacgacga caagcatctc gaaaaaacgc ggaagacgaa ttttcaagga
    28321 ttggagcttt tcgccaagga acttccgaag atcgcgggcg gactcgaaat ggttccgtcg
    28381 tacgccaatt tctttctggt gaaaaccggc aacggacggc aggtcttcgg cgaactgcag
    28441 aaa
//
LOCUS       DAOPZO010000010        27431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_1721_length_27431_cov_8.980165, whole genome shotgun sequence.
ACCESSION   DAOPZO010000010 DAOPZO010000000
VERSION     DAOPZO010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 27431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27431
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1721_length_27431_cov_8.980165"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..105
                     /locus_tag="PKI68_01520"
     CDS             <1..105
                     /locus_tag="PKI68_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52404.1"
                     /translation="QIKTGSLSRSDRISKYNQLLRIEEMLGTEARYGC"
     gene            230..574
                     /locus_tag="PKI68_01525"
     CDS             230..574
                     /locus_tag="PKI68_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52405.1"
                     /translation="MSPQKYWNKIYRSAAIAAIGLALTGVVFAFLPKVTQFRSYQETK
                     NALDADIRAKEEAIKELRLKQERFSTDKYFVQQIAHEIGFAHPGETIYQFNEKAAGAS
                     NPPPAATGEKAP"
     gene            571..1449
                     /gene="thiD"
                     /locus_tag="PKI68_01530"
     CDS             571..1449
                     /gene="thiD"
                     /locus_tag="PKI68_01530"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNX52406.1"
                     /translation="MIKKPIVWTVAGSDSGGGAGVQADIKVINLLGAHDCSAITALTA
                     QNTIGTGRLEWVAPDMLSAQLEALKADLPPAAVKLGMLGSAATVEMVAAFIKTAKSPF
                     TVCDPVLISSSGAFLLEASAWKLLTEKLFPVVNLITPNIPEAEHLLGMEIATPADIEA
                     AAQKALTTGVKEVLIKGGHGSHDMELSSDYWSDGKNHAWLSSPRIDTKHSHGTGCVLS
                     TAIATARALGYGPLDSIIVAKSYLNQGLRQAPGLGSGFGPLAFNPWEKAAQDFPNISQ
                     KADEPGVRAGLPVIGK"
     gene            1540..1617
                     /locus_tag="PKI68_01535"
     tRNA            1540..1617
                     /locus_tag="PKI68_01535"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1575..1577,aa:Pro,seq:ggg)
     gene            1682..2632
                     /locus_tag="PKI68_01540"
     CDS             1682..2632
                     /locus_tag="PKI68_01540"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNX52407.1"
                     /translation="MHKQTVSVTETAAALAGLYSTGHEDRADNVLWNYPAARRVVEAL
                     KILIHVMFPSLYPSEKEELETYFEKQLKEVSHLLLPELERAIPFRWQSESARRAGTQA
                     LENIPAETRRVLNEFLARLPAVREMIIDDVKAAYNGDPAALSYAEVKLAYPGILAVTS
                     HRLAHELYRLDVPIIPRIMNEWTHAKAGIDIHPGATIGRGFFIDHGTGVVIGETAKIG
                     QGVKIYQGVTLGAKSFPLDEHGNPIKHIQRHPTVEDRVIIYANTTVLGGKTVIGHDSV
                     IGGNVFLMDSVPPHSFVTKTEAGVIVRTKDTAGLLGGLGI"
     gene            2718..2915
                     /gene="rpmI"
                     /locus_tag="PKI68_01545"
     CDS             2718..2915
                     /gene="rpmI"
                     /locus_tag="PKI68_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNX52408.1"
                     /translation="MPKLKTKKTAAKRFKKTGTGKLKYSHMGGSHILTGKSRKQRRRL
                     GGTNLVAKTDEKRISKTIPYA"
     gene            2935..3291
                     /gene="rplT"
                     /locus_tag="PKI68_01550"
     CDS             2935..3291
                     /gene="rplT"
                     /locus_tag="PKI68_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNX52409.1"
                     /translation="MPRATNAPASRRRRKNRLELAKGFYGNRSKLFRQATEAVDRAQA
                     MAYVHRKQKKRTFRRLWTVRVNAACKANGISYSRFIEGLAKANVTLNRKMLSEIAIYD
                     AAGFIKLVQTAKAAIA"
     gene            complement(3378..4502)
                     /locus_tag="PKI68_01555"
     CDS             complement(3378..4502)
                     /locus_tag="PKI68_01555"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNX52410.1"
                     /translation="MRTLNRYIVFDYLVIFLAALGLITFVMTVGALVKAVDLMARGIS
                     AALIAKFFFQNIPYILSFSMPISTLFAALLLFGRLSMDSEISAMKSCGISLWRLAAPL
                     ILLSILFSGICVYINSEVAPVAKYANRELLRTAGVEEPVNLLEEGRFIRDFPGLMIYV
                     GRKNGTVVKDVVAYEVDKTGVIKRSVRAKQGDIVADNVNRILTVKLYNVRIEMPDAKD
                     PHDVSKTTYVNAEYYPIKLDFEKLLKSKVTAKKRGHLTMGELVDRIRNVERDFPLLSA
                     PDRLIEKTRLIVEANQRISIAVGCFSFMLIGIPLGVKSHRKETSAGMLVSLIIVFAYY
                     IFIVVAKALADYPWLHPNLILWIPLVVAQFVGLWLVRRSS"
     gene            complement(4507..5394)
                     /gene="folD"
                     /locus_tag="PKI68_01560"
     CDS             complement(4507..5394)
                     /gene="folD"
                     /locus_tag="PKI68_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNX52411.1"
                     /translation="MSAKILDGKAIAQDIRAELKARTAELKTQGIVPGLGVLLVGDDP
                     ASRSYVTAKEKACEETGLYSREIKLPATASHKEILDVVKAFNADEKIDGILVQLPLPD
                     SSMEQSVIEAINPDKDVDGFHPVNVGRMMLGLPAFLPCTPHGVLQILKRSGIKTSGAH
                     VVVIGRSNIVGRPLVNLLSQKSELGDATVTMCHTRTKNMKELTLQADIIVAAVGRPNT
                     VTADMVKPGAVVIDVGVNRVADPSAKAGFRLKGDVDYAAVAEKASAITPVPGGVGPMT
                     ITMLLANTVESAVRRYGAK"
     gene            5457..5753
                     /locus_tag="PKI68_01565"
     CDS             5457..5753
                     /locus_tag="PKI68_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNX52412.1"
                     /translation="MEWIRETTKGVLLPVRAVPRASKNEIQGVHGDALKVRLQAPPVE
                     GKANQALIRFLSEALDIPRSQLSIASGETGRNKAVLITGITREALISLLMNKMQ"
     gene            complement(5725..6465)
                     /locus_tag="PKI68_01570"
     CDS             complement(5725..6465)
                     /locus_tag="PKI68_01570"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HNX52413.1"
                     /translation="MKMKIPPKAYENIPFLNSDPCRPVRLQLEYLYPEVMMEKEKIKS
                     TIVLFGSARIPAPEKADACSNRKLAELTPFYEEARKLARLVSTSCQTNHECEYVIITG
                     GGGGVMEAGNRGAQESGCKSISLNITLPHEQLPNPWVTQDLCFQFHYFSMRKMHFLLR
                     AKAVCIFPGGFGTFDELFEALTLIQTGKIERMPVILFGTEHWQRIVDWNYMAEYGLIG
                     PNDLDLLTFCDTAEDAWNAITAFYSSAG"
     gene            6506..7597
                     /locus_tag="PKI68_01575"
     CDS             6506..7597
                     /locus_tag="PKI68_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52414.1"
                     /translation="MKPVAYYITAHGYGHGTRSCDVLRALHRLYPEQPVIVTSDLPRD
                     FLDSRLKGCPNITVRRGAFDVGLIQKDSIQSNLPETLKALETLYAREELFIEQERIFL
                     EESGAAMVIADIPAIPLAAAQRAGIRNAAVGNFSWDWIYEAYVPQNPRWAFFAEKFRA
                     VYENTGLLLRLPFAPPMEQFPNRKNIPLLASPGVPQREKLAALTGAAIQKQWVLLSFT
                     ALDINNRALENIRALSREYEFFCVQPLNFSGSCIHAVDRAQVCFANVLASCDIIISKP
                     GFGLVSECIVNRKPLIYSDRGDFAEYPYLVEGIERYLRNVHLPLAQLYAGDFSHALKK
                     IKSAPEPEEHLASGGAEMIVQAIIRSGES"
     gene            complement(7575..8084)
                     /locus_tag="PKI68_01580"
     CDS             complement(7575..8084)
                     /locus_tag="PKI68_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HNX52415.1"
                     /translation="MKIGIISDTHGQVDLALAAAREFIFRAVDAVIHCGDVGSDMVLT
                     EMAALFQTLDIPIYAVLGNCDIQEGLGCFRDEPGINLMGRVAHLRLADRRIAVLHSDD
                     EAHFEALVRSNSYDYVFFGHSHTRRDERIEKTRIINPGSAGRGMHPSCAIVNLVDDNV
                     TFFTIHRSE"
     gene            8174..9226
                     /gene="argC"
                     /locus_tag="PKI68_01585"
     CDS             8174..9226
                     /gene="argC"
                     /locus_tag="PKI68_01585"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNX52416.1"
                     /translation="MKSVKIVGAGGYGGVGITELLLRHPEFKIDCLVAATETGMKMSD
                     LYPHLKGFCDDLIRTPDDPKAQEAYDAVFFSTPDGVGMQTAAAELAKGAHVVDYSGDF
                     RFTTPEKYSAYATFIGKNPVHAAPELLPQTVYGLSELHPFGKKQKLAGNPGCFAVSCI
                     LGLAPAAAAKLIDPKSVICDCKTGVSGAGKKPAATFHYPARYEQINAYKLAGHQHMVE
                     IEQELSALAGEPIRITMTAQVLPLCRGILSCLYANLNETMSAKQLMEAYKTFYAGKPF
                     VRIFDNKAAIGTAQVNGTNFCNLIVDVDERNNRLRVISHIDNLMKGQAGNALQNMNLM
                     FGFDPMLGLNFPGRCP"
     gene            9236..9460
                     /locus_tag="PKI68_01590"
     CDS             9236..9460
                     /locus_tag="PKI68_01590"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HNX52417.1"
                     /translation="MISTLHRGLWLTAGLVSLMIGIIGLLLPIVPQLPFFLAAIICFM
                     RCSARFRAWMEKKKWFIRLHTRFRHMRHRQ"
     gene            9518..10936
                     /locus_tag="PKI68_01595"
     CDS             9518..10936
                     /locus_tag="PKI68_01595"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNX52418.1"
                     /translation="MEPKVILFDIAFLLGVPALILAGLIIRPFKKFLMAVMCFSLCYP
                     EQLSMNLMSREGYRMATRGFELGLFDICAIALFFIMVIQPRGHRFRWFPPLTIVYTVY
                     IVTAIISWMLCPDKIHVPADVYAANTAGGDTFYDYFETGLYPLFEISKIIRGAFAYLV
                     VVNFLRDEEHFRTLIGALLIVAFVITFEALVGRYIKGYHRISATLGHPNSLGTFMGMM
                     GTLMFGMALSKPTFMSSGTFAMATAGALVSVLLTISRGALSSLVLGLWMDVSALFHRY
                     LNIKNFTILFFGSLVALGIFFVAADTLSARFLVQQDAVSDIEYRGLYNQEAKLMAQDH
                     AFGVGLGNFSTYSWIKYGPAVKLNEYGTPAHNLWYLTLGELGVPGLIAFIFFWLRFYS
                     IGLPFLFRRRRDLIYAVAASATAASMIGHIQNMLQLSYRQTSIYMFNQILIGLVVAAW
                     YIDRDTRRAERNARRLARSSAA"
     gene            10936..12111
                     /locus_tag="PKI68_01600"
     CDS             10936..12111
                     /locus_tag="PKI68_01600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX52419.1"
                     /translation="MEKQRMPARKKIVCYDDNPDYGGHQVMACLAVEALARSAAWQPI
                     FFCSPENVRLGKRLKEIRTATGNLEIRATPFTTRKLQGLRNRFAKASIGALRRLLESA
                     HPDLILCIQGEIEDGSLALCAAENMTAPCISYIPVPHRMALMGAKLGALRDLLNSRLF
                     KKPDGWITISESMKILLEERGAETPVEVVHNGIDMNRFAPHSKTAARAALGLPENKTL
                     FGAIGRIEFNQKQQDFIVRTFFSHWKNNPALHLVITGDGPDRGRLETLIGRLGLQKQV
                     TLLPWQENSTLVYSALDLLIIPSRFEGVPLVMLEALACGTPVIGSARDGMKDLLPAGW
                     TFETENGNALADVAGYVSEHWPTALPALQAKVRNEYTVEKFQQNFEAALHRFLIMDR"
     gene            12111..12944
                     /locus_tag="PKI68_01605"
     CDS             12111..12944
                     /locus_tag="PKI68_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX52420.1"
                     /translation="MYLDFFQLKEFPFNVTPDPRFLFFSGRHREAFESLLYGIEHRKG
                     FIVLTGEVGCGKTTICRSVLNNLPGTTHSAMILNPSISAAQLIRSILHDLGIEPKGGD
                     KLVHIAQLNRFLLHCLEKGENVAIIIDESQNLDPRLMEQVRMLSNLETDQHKLMQIIL
                     SGQPELKARIQTPELRQLRQRVMVHCDLEPLSVVETTLYIQHRLTVAGAADPARVFQS
                     AAVEKIHARTGGIPRQINTVCDRALLSAYVRNSREVTAADVETALTELASLIGVQGDW
                     Q"
     gene            12941..13522
                     /locus_tag="PKI68_01610"
     CDS             12941..13522
                     /locus_tag="PKI68_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52421.1"
                     /translation="MSLIQEALKRQAEEQPAVILPKLPIDPIAPPPDRKSRKARLIVP
                     LILLLIVFLALPLGYNFFLAKPKHKSLPPAAQKAPAPAAESARPAADSKPVAAKEAPV
                     PEANVAANISPESKSVTITVKKEEPVSETKQAWPELKLTGIAQGESQSIAILNGKMLT
                     AGRTLGDVTVKEVHTADVVVEYRGERRVLYLNE"
     gene            13593..14537
                     /locus_tag="PKI68_01615"
     CDS             13593..14537
                     /locus_tag="PKI68_01615"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNX52422.1"
                     /translation="MNYILQILFILFLMALGAIARRRGIVTEAGTSEMTRLLISFIYP
                     ALIFYSITRLHPEQLARNWILPAMTLVMAGTGFALGLLALRWLKQVDHKRASAFLFQS
                     TINNYLFLPLPLVMMMWGTEGVALLVFASMGFELAVWTVGVFLFNRSAKLADGLRIMF
                     GPPLVTLIVSIIWICLRDLSPFRLPESALLQRVLELLYSGAETIGRATVALSMVVAGS
                     RIAVLKTRAVRDPHVWIVSALRLAATPVLFILILRHIPMENLARNILTVVAVMPAAVA
                     SMIFSERFSGDTDFIAATLLVTHLGAILTVPLLLAWAL"
     gene            14568..15032
                     /locus_tag="PKI68_01620"
     CDS             14568..15032
                     /locus_tag="PKI68_01620"
                     /inference="COORDINATES: protein motif:HMM:NF018958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HNX52423.1"
                     /translation="MRNNDILRRLRYTFDLKDSKMIDLFALADRQVTWEQINAWLKKE
                     DDPAYKECSDLQLAAFLNGLIIDKRGRKEGPQPAPEPSLSNNVILMKLRIALNLKAED
                     VLEIMGLADMPISKHELSAFSRKPDHKHYRVCQDQLLRNFLKGLQIKYCNGR"
     gene            complement(15078..15737)
                     /locus_tag="PKI68_01625"
     CDS             complement(15078..15737)
                     /locus_tag="PKI68_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52424.1"
                     /translation="MKAKKIIFSFVVIAVFTTIYIAYTRSRIISDFRVYDPPFCLYDV
                     GINQSFNEIDCNDFMSVMMGVMHVTNEACEKVVFHSKEWDKLANFRRMGRELSAKERQ
                     DGAKGMYCHRSFKYKSLDGQDVAVILWYLPKFQGGILFTDTMIKENGFWKKTSGPDKY
                     AEDIYRFANLKFDFTVTNIPTDARYFEKIIRQIRADIIKKRNAGNELSAPTNCVDGAK
                     L"
     gene            complement(15826..17601)
                     /locus_tag="PKI68_01630"
     CDS             complement(15826..17601)
                     /locus_tag="PKI68_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX52425.1"
                     /translation="MHTRRIFWQIYPGHLTVTLFTLLVCFAVFTSALERFYRGQVAEN
                     LESLALFTAEQVRDRFEPGQQDYLTAVSARLGTKSPARVTLILPDGTVAGDSARPGEP
                     MENHRDRPEVKTALEQGRTGQAIRFSTSLRKNMFYVAVPVYRDGALIGVVRTAMPLLR
                     MHEVLRGIEHSLLWGGVLILLMAALLSLFVSRRISRPLERIKQGAEEFAAGNFSFRLR
                     SPGSAEVSGLAETMNEMAAQLEERLQTLTKERNQREAVLSSMVEGVLAVDVTGHIISM
                     NKATAQFFNVFQPETAPGRSMEEVFRNVNLQRFVADVLEGQETRECELVIQNRHACYL
                     QTRGSNLLGIQGRRIGAVVVFNDVSRLRRLENLRRDFVANVSHELKTPITAIKGFVET
                     LLDGALKDPVEAERFLKIVAKHADRLNAIIEDLLMLSRLEQDGKAGMEMQKAGLCGLV
                     NSAVEVCAQRAGKKNITINVECSDALTAIINPPLIEQALINLIGNAVKYSADGKTVTV
                     SVHAEEGGVLLSVTDQGCGIEEKHLDRLFERFYRVDKGRSRQEGGTGLGLSIVKHIAQ
                     AHGGTVSVQSRYGEGSTFSIFLPAA"
     gene            complement(17604..18320)
                     /locus_tag="PKI68_01635"
     CDS             complement(17604..18320)
                     /locus_tag="PKI68_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX52426.1"
                     /translation="MNEKIMAAVKTQILIVEDEEDIRELVRYNLERENFSVTEAESGE
                     AGLKSVAQKKPDLILLDIMLPGKDGLQICRELKKNDAACDIPVVMMTARGEESDIVTG
                     LELGAEDYIVKPFSPKVLVARVKAVLRRKAAVPVPDAEDVLSICNIVIHPGRHEVTVG
                     GKPVDLTASEFGILHFLARRPGWVFTRYQIVDAVHGEDYPVTERSVDVQVVGLRKKLK
                     KAGEMIETVRGIGYRFKDEE"
     gene            complement(18313..18903)
                     /locus_tag="PKI68_01640"
     CDS             complement(18313..18903)
                     /locus_tag="PKI68_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNX52427.1"
                     /translation="MSSKNLTHLIERYPELESCREPVLSAVKAIKESLNAGGKLLICG
                     NGGSGADAEHWAGELLKRFCKTRPLSAALPPELAAELEGAFPVIPLTSFPAFQSAWAN
                     DRNPDFNFAQLVNALGQKGDVLIGLSTSGNAKNVCHAVATARALGLKTVGLTGGKGGG
                     LAAAADIAIKVPSTETFKVQELHQPVYHCICLMLDE"
     gene            complement(18963..19631)
                     /gene="phoU"
                     /locus_tag="PKI68_01645"
     CDS             complement(18963..19631)
                     /gene="phoU"
                     /locus_tag="PKI68_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNX52428.1"
                     /translation="MSHVFMNEVERLKKQILSLSAQVEESLILAVKALLTRDEELANQ
                     VIRGDFDVDQVEVEVEEEALKILALYQPVAMDLRFLTAVLKINSDLERIGDLATNIAK
                     RAVKLCKVPAAPVPEQMTESATRTRDMVHDSLNAFVCMDAGLARAVCGSDDRIDDLCR
                     DVRRFVEAQILKDPAHLSSYLDILLASRNIERIGDHATNIAEDVIYLVEGIIVRHRPT
                     DRKS"
     gene            complement(19642..20505)
                     /gene="pstB"
                     /locus_tag="PKI68_01650"
     CDS             complement(19642..20505)
                     /gene="pstB"
                     /locus_tag="PKI68_01650"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017789372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNX52429.1"
                     /translation="MDSMTVQSPPKKKANRLAEIAAGKTFPTETHDALKHEEALLDVQ
                     GFNLHYGAKQALFDINMIIPKGKVTALVGPSGCGKSTLLRSINRINDLLSTVRINGGM
                     MLNGEPVYAPSVDVIELRKRMGMVFQKPNPFPMSIYENVIYSLRIDGERNKSVLDAVC
                     ERSLRGAALWDEAKDRLHESALGMSGGQQQRLCIARAIAAEPEVLLMDEPCSALDPIA
                     TNKIEDLIADLRGGYTIVIVTHNMQQAARVSDYTAFMYLGRLIEYGPTSTIFQNPHLQ
                     ETQDYVTGRFG"
     gene            complement(20517..22199)
                     /gene="pstA"
                     /locus_tag="PKI68_01655"
     CDS             complement(20517..22199)
                     /gene="pstA"
                     /locus_tag="PKI68_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNX52430.1"
                     /translation="MSLQPAEEKTVKPRRRHAKVSLFAQGEPMIWLTGGGLAVALCMI
                     TGLLLLIFYNGIATLWPVPVPQIQTVSNQVYMGEITRDESFRSNEEPVRVLHRKLVRT
                     GNYEISGEHFTWVNRHEIKSETLPEWALVVERTAWGRFYGFPEGFTLGGEAVASTPEA
                     AWKAFNRYHGEVSDRRAKIRALETRDVGRVNRQLEKARLDLRTVELRDGLSSPAWQAE
                     HAKFLETEKSVTTEFKKLQDEIGLLRTENSRYVLNLKTAQGKAVAMPLETIVRAYPAN
                     QLTLAGKTGIYFSRWHEFLCDEPREANSEGGVFPAIFGTVIMTLLMSLAVVPFGVMAA
                     LYLREYAKPGLIVSAVRISINNLAGVPSIVFGVFGLGFFCYLIGASIDELFFASSLPN
                     PTFGKGGILWASLTLALLTLPVVIVATEEALAAVPNSMREGSYACGGSKWQTIKRIVL
                     PRALPGIMTGMILAMARGAGEVAPLMLVGAVKLAPELPVDGVAPFVHAERSFMHLGFH
                     IYDLGFQSQNSEAAKPMVFTTTLLLIVLIFLLNLLAVRLRTRLRRKFVSASF"
     gene            complement(22196..24772)
                     /locus_tag="PKI68_01660"
     CDS             complement(22196..24772)
                     /locus_tag="PKI68_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNX52431.1"
                     /translation="MTQSFTGRARRKKTHWSVRASDRFSKSLITVGGIGTILAVSTVF
                     FYLLYVTVPLFRPARIDGVETLNRPAADSAKPFSIQSDEYGVMNRILYADGRMVCTRL
                     DTGAVISEQMLFTNAALTAFTQDAQTSEVLCGFADGSIQMGRITFLTSYVDAKDLPPE
                     LRTLEEAKTGEWNGTLIQRISAEQFRSIKLSAVFDEPVPGETPSAVRLLDQINSPAGQ
                     ISVMLNEEGTLSLLRFTRRENIMTGKVTVRRTAAVIPYNTKDHREPPAWVLLSGLGDT
                     VCAAWKDGTLLRFDTRNFMNPSLIERIDLVPEPDRKLTSLQLLLGRTTLLAGDSSGRV
                     QAWFPTLSPNGSSQTSLTAAHTFHGSAAVTALASSSRIRMFAAGYADGTVDLFHVTSQ
                     KQLAHLAAGDAPVQALALAPKDDGLLALTPKHLVQWNIDPRHPEVTPAALFLPVWYES
                     AAKPEHVWQSSSGTDDFEPKLGLMPLIFGTLKATLFSMLFGVPVALLAAIFTSEFLSP
                     RAKMHIKPAVEMMAGLPSVVLGFIAALVLAPIVAGMLTVFMTVFITVPASFLLGAYIW
                     QIIPQHWTLRLARFRLLFMLLAVPVGLGLARIVSPLIEKLCFAGDIMRWLDGQIGTAF
                     GGWVLLMIPVSCLLVAWLFSLVIDPRLRFFSMNWSRLQCALFDLGKFIFGFVFAVLLA
                     LAAAALFQSLGFDPRGGLFGTYVQRNALVVGFVMGFAIIPIIYTISEDALSSVPDHLR
                     SASLGAGATPWQTAVRIIIPTAMSGLFSAIMIGLGRAVGETMIVLMAAGNTPVMEWNI
                     FNGFRTLSANIAVELPEAVRNSTHYRTLFLAALVLFVLTFILNTIAELIRMRFRKKAV
                     EL"
     gene            complement(24851..25828)
                     /locus_tag="PKI68_01665"
     CDS             complement(24851..25828)
                     /locus_tag="PKI68_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNX52432.1"
                     /translation="MKKISLSAALIAAAFVTAQAQVQVDAGLPDYKAVQGVSGTIKGI
                     GSDTMNNMMTLWAEGFKKMYPNVQVEIEGKGSSTAPPALIEGTATFGPMSRPMKQMEI
                     DGFVAKYGYKPTELPTSIDMLAVYVHKDNPIKALSLTQVDAIFSKNRKLGNPEDITGW
                     GQLGLKGEWANLPISLYGRNAASGTYGYFKENALGKGDYKDSVKEQPGSSAVVQGVAT
                     DKGGIGYSGIGYKTADVRAVPLAQDAFSAPVEATPENAYSGEYPLARYLYLYVNCKPG
                     SELDPLRREFIKYIFSKQGQTDVVKDGYYPVPANIARKTLESIGIKAGF"
     gene            26057..26239
                     /locus_tag="PKI68_01670"
     CDS             26057..26239
                     /locus_tag="PKI68_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52433.1"
                     /translation="MKLQQGQLWKKDGEFFRIVECERLSVTYKVMANPFAKEGVQHQV
                     TKKEFCRLIKDAKLLP"
     gene            26250..26489
                     /locus_tag="PKI68_01675"
     CDS             26250..26489
                     /locus_tag="PKI68_01675"
                     /inference="COORDINATES: protein motif:HMM:NF021452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 domain-containing protein"
                     /protein_id="HNX52434.1"
                     /translation="MDDAPIKIKLSPPQKALLRQKLIAMFLDDFDEELSDFKADQILD
                     TFIEKLGPGIYNAAIEDMKIFMMHQLEDLDAIFQK"
     gene            complement(26505..27338)
                     /locus_tag="PKI68_01680"
     CDS             complement(26505..27338)
                     /locus_tag="PKI68_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HNX52435.1"
                     /translation="MATIGQINRLQVLRSTDFGLILDGGSLGDILLPKRYVSKEWHLD
                     DIIEVFVMIDSEDRLTATTLRPVAMVGEFACLRVASATRIGAFLDWGLPKDLFVPFRE
                     QKVEMREGQSYVVRIYLDETSGRIAASAKLDKFLDRTPSNYKTGDKVRLLVCDKTDLG
                     FKAIINGRHWGILFYNEVFQPLERGQKLDGFIKQVRGDGKIDLCLQKPGFEKVTELTD
                     VILNYIKAQGGFMPVTDKSPPEEIYRLFGVSKKTYKQAIGALYKKRLITFEEKGTRLA
                     K"
BASE COUNT         6112 a         8178 c         7245 g         5896 t
ORIGIN      
        1 cagatcaaga ccggctcgct cagccgctcc gaccgtatca gcaaatacaa ccagctgctg
       61 cgcatcgaag aaatgctggg aactgaagcc cgctacggct gctaacgttc atcagccgta
      121 ctaaaaggca ccccgggatt tcccggggtg tttttttatc ctgcggcagc cgccaaaatt
      181 tcgtatttca tatttcataa tttatcctga tccccctata ctgcgcgccg tgagtcctca
      241 aaagtactgg aataaaattt accgctccgc agcgattgcc gcgattggac tcgcgttgac
      301 cggtgttgtt ttcgcctttc tgcccaaggt gactcagttc cgcagttatc aggaaaccaa
      361 gaacgcactg gacgcggaca tccgcgccaa agaagaagcc atcaaagaac tgcgccttaa
      421 gcaggaacgg ttcagcaccg ataaatattt tgtgcagcag atcgcccatg aaatcggttt
      481 tgcccacccg ggcgagacga tctaccagtt caacgaaaaa gccgccggcg cgagcaatcc
      541 gccgccggcc gcaaccggag aaaaagcacc atgatcaaaa aaccgatcgt ctggaccgtg
      601 gcaggctcgg attccggcgg cggtgccggt gttcaggccg acattaaagt catcaacctg
      661 cttggcgcac acgactgctc agcgatcacc gcactgacgg ctcagaatac catcggcacc
      721 ggccggcttg aatgggtcgc accggatatg ctttccgccc agctcgaagc gctcaaggct
      781 gatctgccgc ctgccgccgt aaagctcggc atgctcggca gcgccgctac cgtcgaaatg
      841 gtcgcagcgt tcatcaaaac ggccaagtcg cccttcaccg tctgcgatcc tgttctgatt
      901 tcctccagcg gcgcgtttct gcttgaagcc tccgcatgga aactgctcac cgaaaaactt
      961 tttccggtcg tcaatctgat cacgcccaat atccctgaag ccgaacatct gctcggtatg
     1021 gaaatcgcca ccccggccga catcgaagct gccgcgcaga aagcgctcac caccggcgtc
     1081 aaagaagttc tgatcaaagg cggacacggt tcacacgaca tggagctttc cagtgactac
     1141 tggagtgacg gaaaaaatca cgcctggctt tcgagtccgc ggatcgacac caaacactcg
     1201 cacggcaccg gctgtgtact ttcaacggcg atcgccacgg cccgtgcgct gggttacggc
     1261 ccgctcgaca gcattatcgt cgcgaagtcc tatctcaatc aggggctgcg tcaggctccc
     1321 ggactgggca gcggcttcgg cccgcttgcc ttcaacccgt gggaaaaagc ggcgcaggat
     1381 tttccgaata tttcgcagaa agcagatgaa ccgggcgttc gcgccggtct cccagtcatt
     1441 ggaaaatagc cgtttttatt ccggataccg gaaaatcatt ccttccggca agtctcgcgt
     1501 tgaccaccca tagggtgcgt gatagcttct tgcgctttac ggggtgtagc tcagcctggc
     1561 tagagcgctt gcttggggtg caagaggccg cgagttcaaa tctcgccact ccgaccatac
     1621 tgcgtccgca gcgcactaaa atcttgcaac cgtttacgtt cccctcttaa ctgcccgcat
     1681 catgcataaa caaactgtat ctgtaacgga gactgccgcc gcactggccg ggctttactc
     1741 gaccggtcat gaagaccgcg cagacaatgt actgtggaac tacccggccg cccgacgtgt
     1801 tgtcgaggcg ttgaaaattc tcattcacgt catgttccca agcctgtatc cgtctgagaa
     1861 ggaagaactg gaaacctatt tcgaaaaaca actcaaagaa gtttcacatc ttctgctgcc
     1921 ggaacttgaa cgcgcaattc ctttccgctg gcagtcagaa tccgcccggc gggccggcac
     1981 acaggctctt gaaaatattc cggcggaaac ccggcgggtt ctgaatgaat ttctggcacg
     2041 cctgccggcg gttcgcgaaa tgatcatcga cgacgtgaaa gccgcttaca acggcgaccc
     2101 cgccgcactg agctacgccg aagtcaagct cgcctatccc ggcatactcg ccgtcacctc
     2161 acaccggctg gcgcatgagc tctaccggct cgatgtgcca atcatcccgc gcatcatgaa
     2221 cgaatggacc cacgccaagg ccggcatcga cattcatccc ggagccacca tcggtcgcgg
     2281 tttcttcatc gaccacggaa ccggcgtggt gatcggagaa acggcaaaaa tcggacaggg
     2341 cgttaagatc tatcaggggg tcacgctcgg cgcgaagagc tttccgctcg acgaacacgg
     2401 caatccgatc aagcacatcc agcgccaccc gacggttgag gatcgtgtta tcatctatgc
     2461 caacaccacc gttctcggcg gaaaaacagt catcggacac gactcggtaa tcggcggtaa
     2521 tgttttcctg atggacagtg ttccgccgca cagttttgtt accaaaaccg aggcgggagt
     2581 gatcgttcgc accaaagata cagccggttt gctgggcgga cttggaatct aacattaaaa
     2641 tataatttaa aagctggact caccccgact gcttacctat atttctgcgc ttttacgtac
     2701 caatttgagg agcaatcatg ccgaaactga aaacaaaaaa gactgcggcc aaacgtttta
     2761 agaaaacggg aaccggcaaa ctgaaatatt cccacatggg cggcagccac attctgaccg
     2821 gcaagagccg taagcagaga cgccgtctcg gcggtaccaa tctggtcgca aaaaccgacg
     2881 agaaacgtat ctcaaaaact attccctacg cttaattttt aaggagtacg aaccatgcca
     2941 agagccacca atgccccggc ctcgcgccgc cgtagaaaaa accgtcttga actcgctaaa
     3001 gggttttacg gtaaccgcag caaacttttc cgtcaggcta ccgaagcggt agaccgcgct
     3061 caggcgatgg cttacgtcca tcgcaagcag aaaaagcgca ccttccgccg cctctggacg
     3121 gtacgtgtta atgccgcctg caaagccaac ggcatctcct acagccgttt cattgaaggt
     3181 ctggccaaag ccaatgtcac tctgaaccgc aaaatgcttt ccgaaatcgc catttatgac
     3241 gccgccggat tcatcaagct ggttcagacg gctaaagccg ccattgctta gcacagcagg
     3301 ttgctgaacg ttgaaaatcc tgaccgcgac agcggccggg atttttgttt ttccagagag
     3361 ttaaaacagt cgtaccgtca gctggagcgc cgaaccagcc acagcccgac aaactgcgcg
     3421 acaaccagcg gaatccacag aatcagattc ggatgaagcc acggataatc cgcaagcgct
     3481 ttagcgacaa caatgaagat gtaatacgca aaaacgatta tcagactgac aagcataccg
     3541 gcagacgttt ctttgcggtg ggatttaacc ccgagcggta taccgatcag catgaagctg
     3601 aagcagccga ccgcaatgga aatgcgctga ttggcttcca caatcagccg tgttttttca
     3661 atcagccggt ccggcgcgga aagcaacgga aaatcgcgct ccacattccg gatccggtcg
     3721 acaagctctc ccattgtcag atggccgcgt ttcttggcgg taaccttgct cttgagcagc
     3781 ttttcgaaat cgagcttgat cggataatac tcggcattga cgtaggtggt ttttgaaacg
     3841 tcgtgcggat ctttggcatc cggcatttca atgcgtacat tataaagctt cacggtcagg
     3901 atccggttga cgttatccgc aacaatatcg ccctgtttag cgcgcacact gcgtttgatc
     3961 acgccggtct tatccacctc ataggccacc acatctttga cgaccgtccc gtttttgcgc
     4021 ccgacatata tcatcaggcc gggaaagtcg cggataaagc gcccttcttc gagcagattg
     4081 acaggctctt ccacgcccgc cgtccgcagc agctcgcggt tggcatactt ggcgaccggc
     4141 gccacctcgc tgttgatgta aacgcagata ccgctgaaca gtatggaaag cagaatcagc
     4201 ggagccgcca gccgccaaag gctgataccg caactcttca tcgcactgat ttcactgtcc
     4261 attgaaagcc gtccgaaaag cagtaacgcg gcaaacagcg tactgatcgg catggagaag
     4321 gagagaatat agggaatgtt ctggaagaaa aatttggcaa tcagcgcggc ggaaatgccg
     4381 cgcgccatca aatcaaccgc tttcaccagc gcgccgacgg tcatcacaaa ggtaatgagt
     4441 ccgagcgcgg ccagaaagat caccagataa tcaaaaacaa tatagcggtt gagtgtacgc
     4501 ataattttac tttgccccgt aacggcgcac cgccgactcc acggtattgg ccagcagcat
     4561 ggtaatcgtc atcgggccga cgccgccggg caccggagta atggccgagg ctttttcagc
     4621 cacggctgca tagtccacat cgcctttcag gcggaatccg gccttcgcgg acggatcggc
     4681 cacccggtta acgccgacat caatcaccac cgctcccggt ttcaccatat ccgccgtcac
     4741 ggtattcggc cggccgactg cggcgacgat gatatcggcc tgcaacgtca gttccttcat
     4801 gttttttgtg cgggtgtggc acatcgtcac cgttgcgtcg cccagttcac tcttctggct
     4861 gagcagattc accagcggac gtccgacaat attgctgcgc ccgattacga cgacatgcgc
     4921 gccggatgtt ttgattccgg agcgttttaa aatctgcaaa acgccgtgcg gcgtacaggg
     4981 cagaaacgcc ggcaggccga gcatcatccg gcccacgttg accggatgga agccgtccac
     5041 gtctttgtcc ggattgatcg cttcgatcac cgactgctcc atcgaagaat ccggaagcgg
     5101 aagctggacg agaattccat caatcttttc gtcggcgttg aacgccttga ccacatcgag
     5161 gatttcctta tgcgatgccg tcgccggcag cttgatttcg cgggaataaa ggccggtttc
     5221 ctcacaggct ttctctttgg ccgttacata agagcgcgac gccggatcgt caccgaccag
     5281 cagtacgccg aggccgggaa cgattccctg cgttttcagc tccgctgtac gggctttgag
     5341 ttcagcgcgg atatcctgtg cgattgcctt gccatctaaa atctttgcgg acattcagtc
     5401 tccttttgga attaacggat gacgggtttt aacctaatgt tccggcggta tcaattatgg
     5461 aatggatccg agaaacaact aaaggcgtcc tgctgcccgt ccgggcggtt ccgcgcgcct
     5521 caaaaaacga gatacagggc gttcacggcg acgccttaaa agtccgctta caggcgccgc
     5581 cggttgaggg taaagcgaat caggcgctta tccgcttcct cagcgaggct ctggatattc
     5641 cccggtcgca gctctccatt gcttcgggcg aaaccggccg caacaaagct gtcctgatca
     5701 ccggcatcac cagggaagcg ctcatcagcc tgctgatgaa taaaatgcag tgatagcatt
     5761 ccacgcatct tcggcagtgt cgcagaaggt gagcaggtcg agatcgttcg ggccgatgag
     5821 cccatattcg gccatgtagt tccaatcgac aatccgctgc caatgttccg tgccaaagag
     5881 gatgacaggc atccgctcaa tcttgccggt ctgaatgagc gtgagcgctt caaaaagttc
     5941 atcgaaagtg ccgaaaccgc cgggaaaaat acagaccgct ttggcacgaa gcaggaagtg
     6001 cattttccgc atactgaaat agtgaaactg aaagcaaagg tcctgagtca cccagggatt
     6061 cggcagctgc tcatgcggca gtgtgatgtt gagcgaaatg gatttacagc ctgactcctg
     6121 cgctccgcgg ttgccggcct ccataacacc gccacctccg ccggtgatga tcacatactc
     6181 acattcgtga ttggtctgac agctggtact gacgagccgc gcgagcttgc gcgcttcttc
     6241 atagaacggc gtcagctcag ccagttttcg attgctgcag gcgtccgcct tttccggcgc
     6301 cggaatccgc gcacttccga aaagcacgat agtcgatttg atcttttcct tttccatcat
     6361 cacttccgga taaagatatt cgagctgcag gcgcaccgga cggcagggat cgctgttcaa
     6421 aaaaggaata ttctcatacg ctttcggcgg aattttcatt ttcatgttct ccatagagca
     6481 tcggttcgat gtaaagataa caaagatgaa accggttgcc tactacatta ccgcccacgg
     6541 ctacggacac ggcacacgct cctgcgatgt gctgcgcgcc ctgcaccgtc tttatccgga
     6601 acagccggtg attgtgacaa gcgatctgcc gcgtgacttt ctggacagcc gcctgaaagg
     6661 ctgcccgaac atcacagtcc gacgcggcgc gttcgacgtc ggtcttatcc agaaagattc
     6721 catccaaagc aatctgcccg aaacactgaa agcactcgaa acgctctacg cccgcgaaga
     6781 actgttcatt gaacaggaac ggatattcct ggaagaaagc ggcgcggcca tggttattgc
     6841 cgatattccc gccattccgc tggccgccgc gcagcgcgcg ggcatcagga atgcggccgt
     6901 cggcaatttt tcgtgggact ggatttacga agcctatgtc ccgcagaatc cgcggtgggc
     6961 gttcttcgcg gaaaagttcc gcgccgtcta cgagaatacc ggactgctgc tgcgcctgcc
     7021 ttttgcgccg ccgatggaac agtttccgaa ccggaaaaac attcccctgc tcgcttcgcc
     7081 cggtgtgccg caacgcgaaa aactggcggc gctgaccggc gcagcgatcc aaaaacagtg
     7141 ggtgctgctc tcatttaccg ctctggatat aaataaccgg gcattggaaa atatccgggc
     7201 gctgagccgc gagtatgaat tcttctgcgt tcagccgctg aacttctccg gctcatgcat
     7261 tcatgccgtt gaccgcgctc aggtttgctt cgccaatgtt ctggcatcat gcgacatcat
     7321 catcagcaag ccgggcttcg ggctggtctc ggagtgcatc gtgaatcgca agccgctgat
     7381 ttattctgac cgcggcgatt ttgcagaata tccctatctg gtcgaaggca ttgaaaggta
     7441 tctgcgcaac gtccacctgc cgcttgcaca gctttatgcc ggagattttt cccatgcgct
     7501 gaaaaaaatc aaatccgcgc cggaaccgga agaacacctc gcctccggcg gcgcggaaat
     7561 gatcgtgcag gcgattattc gctccggtga atcgtgaaaa aggtgacgtt atcgtcaacc
     7621 agattgacga ttgcacagga aggatgcata ccgcgcccgg ccgaacccgg attgatgatg
     7681 cgtgtctttt caatccgctc atcccggcgg gtgtgcgagt ggccgaaaaa aacatagtca
     7741 tagctgttgg atcgcacgag ggcctcaaaa tgcgcttcgt cgtcactgtg caggacggca
     7801 atccgccggt cggccagtct gagatgagcc acccgtccca tgagattaat accgggttcg
     7861 tcgcggaagc agcccagtcc ctcctgaata tcgcaattcc cgagcacggc ataaatggga
     7921 atatccaaag tctggaagag cgcagccatt tcggtaagaa ccatatccga gccgacatcg
     7981 ccgcaatgaa taaccgcatc gaccgcacga aagatgaatt cgcgggcggc agccagcgca
     8041 agatccacct gaccgtgcgt gtcggatata atgccgatct tcacttgtgt gtcccttctc
     8101 tttagttctt ggcacggaaa ccgaaggggc tatattagcg cgattctgaa tcaatgcgag
     8161 cggagatttt aaaatgaaga gcgtaaagat tgttggagca ggcggttacg gcggtgtggg
     8221 tattacagag ctgctgctgc ggcatcctga atttaaaatc gattgtctgg tggccgccac
     8281 cgaaaccggc atgaagatga gcgatcttta ccctcacctt aaagggttct gcgacgacct
     8341 gatccggaca ccagacgatc cgaaagcgca ggaagcctat gatgccgttt tcttttccac
     8401 gcccgacggt gtcggcatgc agaccgccgc cgccgaactg gccaaaggcg cgcatgtggt
     8461 ggattacagc ggcgacttcc ggtttacgac gccggaaaaa tacagcgcct acgccacatt
     8521 tatcggcaaa aaccctgttc atgccgcacc ggaacttctg ccgcagacgg tatacggcct
     8581 ttcggaactc catccgttcg gcaaaaaaca gaaactggcc ggcaatccgg gctgtttcgc
     8641 cgtcagctgt attctcggtc ttgcgcccgc agcggcggca aagctgatcg atccgaagag
     8701 cgtcatctgc gactgtaaaa ccggggtttc cggtgcagga aaaaaaccgg ccgccacctt
     8761 ccattacccg gcccggtacg aacagatcaa cgcctataaa ctggccggcc accagcacat
     8821 ggtggaaatc gagcaggagc tgagcgctct ggcgggtgaa cccatccgga tcaccatgac
     8881 cgcgcaggtt ctgccgctct gccggggcat tctctcctgc ctttacgcca acctcaacga
     8941 aaccatgagc gcgaaacagc tgatggaagc ctataaaacg ttctatgccg gcaaaccgtt
     9001 cgtgcggatt ttcgacaaca aagccgcgat cggcacggcg caggtcaacg gaaccaactt
     9061 ctgcaacctg atcgttgacg tggatgaacg gaacaaccgg ctgcgggtca tctcacatat
     9121 cgacaacctg atgaaaggtc aggccggcaa cgcgctgcag aacatgaacc tcatgttcgg
     9181 cttcgacccg atgctgggcc tgaattttcc gggacgctgt ccgtaaccgg ccgttatgat
     9241 cagcactctc catcgcggac tctggctgac agccggcctt gtttctctga tgatcggaat
     9301 catcggcctt ctgctgccga ttgtaccgca actgccgttt tttctcgcgg cgatcatctg
     9361 ctttatgcgc tgttcggccc ggttcagagc atggatggaa aagaagaaat ggtttatccg
     9421 gctgcacaca cgcttccgcc atatgcggca tcggcagtaa gtcaaaacgg aaagccgttg
     9481 tcggaggatc gttcttccgc tagactgccc gcgttttatg gaacccaaag taatcctatt
     9541 tgacattgcg tttctgctcg gggtgccggc actcatcctg gccggactga ttatccgtcc
     9601 gttcaaaaaa tttctgatgg cggttatgtg tttttcgctc tgctatccgg aacagctctc
     9661 catgaacctg atgtcgcgcg agggttatcg catggcaacc cgcgggttcg agctgggcct
     9721 ttttgatata tgcgccattg cactcttttt catcatggta attcagccgc gcggacatcg
     9781 cttccgctgg tttcctccac tgaccatcgt ttataccgtc tacatcgtca ccgccatcat
     9841 ctcgtggatg ctgtgtccgg ataaaattca tgttccggcc gacgtttatg ccgccaacac
     9901 cgctggcggt gacacttttt atgactattt tgaaaccgga ctctatccgc ttttcgagat
     9961 cagtaaaatt atccgcgggg ccttcgcata ccttgtcgtc gtcaattttc tgcgggatga
    10021 agagcatttc cgcactctga tcggcgcact gctcattgtt gccttcgtga ttaccttcga
    10081 agcgctggtc ggccgctaca tcaaaggata ccaccgaatc tctgcgaccc ttggacatcc
    10141 gaacagtctg ggcacattca tgggcatgat ggggactctc atgttcggca tggcgctctc
    10201 caagcccacc tttatgtcca gcggaacatt cgcgatggcc acggccggcg cgctggtttc
    10261 cgtactgctc accatttcgc gcggcgcgct gtcctcgctg gtactcggac tctggatgga
    10321 tgtctccgcc ctgttccacc gctacctgaa catcaaaaac ttcaccatcc tcttcttcgg
    10381 atcgcttgtc gcacttggca tctttttcgt ggcggccgat accctctcgg cccggttcct
    10441 tgtacagcag gacgccgtgt cggacattga ataccgcggt ctgtacaacc aggaggcaaa
    10501 actgatggcg caagaccatg ccttcggtgt cggtcttgga aacttttcaa cctattcctg
    10561 gatcaaatac ggcccggcgg tcaaactcaa cgaatacgga acacccgcgc ataatctctg
    10621 gtatctcacg ctgggcgaac tgggcgtccc cggtctgatt gccttcatct ttttctggct
    10681 ccggttctac tccatcggcc tgcccttcct tttccgccgg cgacgcgacc tcatttatgc
    10741 ggtcgccgct tcggcaacgg ccgccagcat gatcgggcac attcagaaca tgcttcagct
    10801 ctcctaccgg cagacctcca tttacatgtt caaccagatc ctgatcggcc ttgtggtcgc
    10861 cgcctggtac atcgaccgcg acacccgccg cgccgaacgc aatgcccggc ggctggcgcg
    10921 cagcagcgcc gcctgatgga aaagcaacgt atgcccgccc gcaaaaagat tgtctgctac
    10981 gacgacaacc ccgactacgg cggtcatcag gtcatggcct gccttgccgt tgaagcgctc
    11041 gcaagatcgg ccgcctggca gccgatcttc ttttgcagcc ctgaaaacgt gcggctcggt
    11101 aaacgcctca aagaaatccg gaccgccacc ggcaatctcg aaatccgcgc aacacccttc
    11161 accacccgca aactgcaagg tctgcgcaac cgcttcgcca aagcctccat cggcgctttg
    11221 cgccggctgc ttgaatccgc tcaccccgac ctgattctct gcatccaggg cgaaatcgaa
    11281 gacggctcgc tcgccctgtg cgccgcggaa aatatgaccg cgccatgcat cagctacatt
    11341 cccgttccgc accgcatggc actcatgggc gccaaactcg gcgccctgcg cgacctgctg
    11401 aacagccggc ttttcaaaaa accggacggc tggatcacca tcagcgagtc gatgaaaatc
    11461 ctgctcgaag aacgcggcgc cgagacgccg gtcgaagtgg tgcataacgg tattgatatg
    11521 aaccgctttg cgccgcacag caaaaccgct gcccgcgccg cgctcggact tccggaaaac
    11581 aaaactctgt tcggcgccat cggccgcatt gagttcaacc agaaacagca ggatttcatc
    11641 gtccggactt ttttctcgca ctggaaaaac aatccggccc tgcacttggt catcaccggc
    11701 gacggtcccg accgcggacg gctggaaacg ctgatcggca gactggggct gcaaaagcag
    11761 gttaccctgc tcccgtggca ggagaacagc acgctcgtct acagcgcact cgacctgctc
    11821 atcatccctt cacgttttga aggcgtaccg ctcgtcatgc tcgaagcgct ggcctgcgga
    11881 actcccgtca tcggcagcgc ccgcgacggc atgaaagacc tgcttcccgc cggctggacc
    11941 tttgaaaccg aaaacgggaa cgccctcgcc gatgtcgccg gttatgtatc cgaacattgg
    12001 ccaacggctc tgcctgcgtt gcaggcaaaa gtccgtaacg aatatacggt tgaaaagttt
    12061 cagcagaatt ttgaagcggc cctgcaccgt ttccttataa tggaccggta atgtacctcg
    12121 acttcttcca gcttaaagag tttccgttca acgtgacgcc cgacccgcgt ttcctctttt
    12181 tttccgggcg ccaccgcgaa gcgttcgaat ccctgctcta cggcatcgaa caccgcaaag
    12241 ggtttatcgt cctcaccggc gaagtcggct gcgggaaaac caccatctgc cggtctgttc
    12301 tcaacaacct gcccggcaca acacactcgg ccatgattct caatccgtcg atctctgccg
    12361 cgcaactgat ccgctctatt ctgcacgatc tcggcattga gccgaaaggc ggcgacaagc
    12421 ttgtacatat tgcccagctc aaccgctttc tcctgcactg tctggagaaa ggcgaaaacg
    12481 tcgccatcat catcgacgaa tcccagaatc tcgacccccg gcttatggaa caggtgcgga
    12541 tgctctccaa tctggaaacc gatcagcaca aactcatgca gatcattctc tccggacagc
    12601 ctgaactcaa agcacgtatc cagacacctg aactgcgcca gctgcgccag cgggtgatgg
    12661 tgcactgcga tctcgaaccg ctctctgttg tcgaaacaac cctttacatc cagcaccgcc
    12721 tcaccgtcgc cggtgccgcc gaccctgcca gagttttcca aagcgccgcc gtcgaaaaaa
    12781 ttcatgcccg gaccggcggc attccccgcc agatcaacac agtctgtgac cgcgcgcttc
    12841 tttccgccta tgtacgcaac agccgcgaag tcacggctgc ggacgtcgaa accgctctga
    12901 cagagcttgc atcattaatt ggagtccagg gagattggca atgagtctga ttcaagaagc
    12961 actgaaacgg caggccgaag agcagcctgc agtcattctg cctaaactgc cgattgatcc
    13021 gatcgcgccg ccacccgaca gaaaaagccg caaagcgcgc ctgattgtac cgctcattct
    13081 gctgctgatc gtattcctcg ccttaccgct tggttacaat ttctttctgg cgaaaccaaa
    13141 acataaatcc ctccctccgg ctgcccagaa ggcaccggca ccggccgccg aatccgcacg
    13201 tcccgccgct gactccaaac cggttgccgc gaaagaagca cccgttccgg aagccaacgt
    13261 tgcggcgaac atttcaccgg agtccaaaag cgttacgata acggttaaga aagaagaacc
    13321 tgtatcggaa acaaaacagg cctggccgga attaaaactg accggcatcg ctcagggcga
    13381 aagtcagagt atcgccatcc ttaacggaaa aatgctcacg gccggcagaa cactgggcga
    13441 tgtgaccgtc aaagaagtcc ataccgccga tgtcgtcgtt gaataccgcg gtgaacgccg
    13501 cgtcctgtat cttaacgaat aatcgccgcg cgttaaaaat cccgattagt ccttccctat
    13561 ccggccggtt ccggtgtttc atagacgccg ttatgaatta cattcttcag attctgttca
    13621 tcctgttcct gatggcgctc ggcgccatcg cccgccgccg cggcatcgtc accgaagccg
    13681 gcacatcgga aatgacccgg ctgctgattt ccttcatcta tcccgcgctc atcttctatt
    13741 ccatcacccg gctgcatccc gaacagctgg cccgcaactg gatcctgccg gccatgaccc
    13801 tggtgatggc cgggaccggg ttcgcactcg gcctgctggc cctgcgctgg ctcaaacagg
    13861 ttgaccacaa gcgggcctcg gcattccttt tccagagcac catcaacaac tacctgtttc
    13921 tgccgctgcc gctggttatg atgatgtggg gaacggaagg cgtcgcgctg ctggtctttg
    13981 cctccatggg atttgagctg gccgtctgga ccgtcggcgt atttctgttc aaccgctccg
    14041 ccaaactggc ggacggcctg cgcatcatgt tcggcccgcc gctggtcacg ctcatcgtct
    14101 ccatcatctg gatctgcctc cgcgacctct cgccgttccg cctgccggag agcgcgctgc
    14161 tgcagcgcgt gctggaactg ctgtattccg gcgcggaaac catcggccgg gccaccgtgg
    14221 cgctgtcgat ggtggttgcc ggcagccgga tcgccgtact caaaacccgc gcggtgcgcg
    14281 atccgcacgt ctggatcgtc tccgcgctcc gtctggccgc gacccccgtt ctgttcattc
    14341 tgatactccg gcacattccg atggaaaacc tcgcgcggaa cattctgact gtcgtcgccg
    14401 tcatgccggc ggccgtggcc agtatgattt tcagcgaacg attcagcggc gacaccgatt
    14461 tcatcgcggc gacgctgctg gtcacccacc tcggcgccat cttaaccgtt ccgctgctgc
    14521 tggcgtgggc gttataacca accacgcatt aaaaaggaca ctgtaccatg agaaataacg
    14581 atattctgcg ccgcctccgc tacaccttcg acctgaagga ttcgaagatg atcgacctgt
    14641 tcgccctggc agaccgtcag gtcacatggg aacagatcaa cgcctggctg aaaaaagaag
    14701 acgatccggc gtacaaagaa tgcagcgacc tccagctggc cgcctttctg aacggactca
    14761 tcatcgacaa acgcggcaga aaagaaggcc cgcagcccgc gccggaaccg agcttaagca
    14821 acaacgtcat cctgatgaaa ctgcggatcg cgctgaacct gaaagccgaa gatgtactcg
    14881 aaatcatggg gctggcggat atgccgatca gcaaacacga actcagcgcc ttttcacgca
    14941 agccggatca caaacactac cgcgtctgcc aggaccagct gctgcgcaac ttcctcaaag
    15001 gtctgcaaat caaatactgc aacggccggt agcccggaaa agcgaagctg cttcagtcga
    15061 agcacggctg aaccgcttca tagcttcgca ccgtcaacgc agttggttgg cgcgctgagt
    15121 tcatttcccg cattccgctt tttgatgatg tcggctctga tctgccggat tatcttttca
    15181 aaatagcggg catcggtcgg aatgttggtc acggtaaaat caaacttcaa gtttgcaaac
    15241 cgatagatat cctctgcata tttatcaggg ccactggttt ttttccaaaa gccattctcc
    15301 tttatcattg tatcagtaaa taaaattcca ccttgaaatt ttggtaaata ccaaagaatt
    15361 accgctacat cctgcccatc cagtgatttg tatttaaagg aacgatgaca atacatgcct
    15421 tttgcgccgt cctgcctctc tttcgccgag agctctcgtc ccattctccg aaagtttgct
    15481 aatttatccc attccttgct atgaaaaact accttctcac aggcttcatt ggttacatgc
    15541 atcacgccca tcattaccga catgaagtca ttacaatcta tttcattaaa agactggttg
    15601 ataccaacgt cataaaggca aaacggcgga tcatataccc tgaaatcaga aataatcctg
    15661 ctgcgggtgt aggcaatata gattgttgta aaaaccgcga ttacaacgaa gctgaagatt
    15721 attttcttgg ccttcatttt acatcctaat ttgatcaacg gtgttagaaa aagtgtccgt
    15781 ccggtaatcc cgtaatcgtt tgttcattcc aaattatctc atcttctagg ctgccggcag
    15841 gaagatggag aaggtcgagc cttcgccgta gcggctctga accgagaccg ttccgccgtg
    15901 ggcctgcgcg atatgtttaa cgatggaaag gccgagcccg gttccgcctt cctggcggct
    15961 gcggccttta tcgacgcggt agaagcgttc gaagagccgg tcgaggtgct tctcttcaat
    16021 accgcaacct tggtctgtaa ccgacagaag cactccgcct tcttcagcat gcacgctgac
    16081 cgtaacggtt ttcccgtccg cgctgtattt aacggcgttg ccgatcaggt tgatgagagc
    16141 ctgttcgatc agcggaggat taatgatggc tgtcagcgca tcggaacatt caacgttgat
    16201 agtaatgttc tttttgccgg cgcgctgagc gcagacttcg actgcggaat tcacaagccc
    16261 gcaaagtccg gctttctgca tttccatacc cgctttgccg tcctgttcga ggcgcgaaag
    16321 catgagcagg tcttcgataa tcgcattgag ccggtcggcg tgtttggcca cgattttcag
    16381 aaagcgttcg gcctcgaccg gatctttgag cgcgccgtcg agcagtgttt cgacaaatcc
    16441 ttttatggcg gtgatcggcg tcttcagttc gtgcgagacg ttggcgacaa agtcccggcg
    16501 caggttttcg agccggcgca gacgggaaac atcgttaaat acaacgactg caccgatgcg
    16561 gcggccctgt attccgagca gattgctgcc gcgagtctga aggtagcagg cgtggcggtt
    16621 ctggataacc agttcgcatt cgcgggtttc ctgcccttca agcacgtcgg cgacgaaccg
    16681 ctgcaggtta acgttgcgga aaacttcttc catgctgcgc cccggcgcgg tctcgggctg
    16741 gaagacgttg aaaaactgtg ctgtggcctt gttcatgctg atgatgtgtc cggtcacatc
    16801 caccgccagt acgccctcga ccatactgga aagcacggcc tcgcgctgat tccgttcttt
    16861 cgtcagcgtt tgcagccgct cttcaagctg cgcggccatt tcgttcatgg tttcagccag
    16921 gccgctgact tcagcggagc cgggcgagcg gagccggaag gaaaaattcc cggcggcaaa
    16981 ctcctctgcg ccctgtttga tccgttcgag cggtctggaa atacgccgcg aaacgaacag
    17041 gctgagaagc gcggccatca gcagaatcag caccccgccc cacagcaggc tgtgctcgat
    17101 gccgcgaagc acttcatgca tccggagcag cggcatggcc gtgcggacca cgccgatcag
    17161 cgcgccgtcg cggtaaacag gcacggcgac gtagaacatg tttttacgga gactcgtgct
    17221 gaaccggatt gcctgccctg ttcgtccctg ctcaagcgcg gttttcacct ccggccggtc
    17281 acggtggttt tccatcggct cgcccggacg ggccgagtca ccggcgacgg ttccgtcggg
    17341 aagaatcaga gtaacacgcg ccggtgactt ggtgccaagc cgggcgctga cagcggtgag
    17401 gtaatcctgc tgtccgggtt caaagcggtc gcgaacctgc tccgccgtaa acagcgccag
    17461 agattccaga ttttcagcga cctgtccgcg atagaaacgc tccagagcag aggtaaagac
    17521 cgcaaagcag accagcagcg tgaaaagagt aaccgtgagg tggccgggat aaatctgcca
    17581 gaagatgcgc cgcgtgtgca tggttattcc tcatccttga accggtagcc gatgcctcgc
    17641 accgtttcga tcatttcgcc ggctttcttc agtttttttc gcagaccgac aacctgcacg
    17701 tcaacggagc gttcggtaac cggataatct tcgccgtgca ccgcatccac gatctggtag
    17761 cgcgtaaaga cccagccggg ccggcgggcc agaaaatgaa gaatgccgaa ctcgctcgca
    17821 gtcagatcaa ccggtttccc gccgaccgtc acttcatggc ggcccggatg aatgacaata
    17881 ttgcaaatgc tcagaacgtc ttccgcatcc ggcaccggaa cggcggcttt tcgccgcagg
    17941 acggctttca cgcgggcgac cagcactttc ggactgaacg gtttgacgat gtaatcctcc
    18001 gcgccgagtt cgaggccggt cacgatatcg ctctcttcgc cgcgcgccgt catcatcaca
    18061 accggaatat cgcaggcggc gtcattcttt ttcagttccc ggcagatctg cagtccgtct
    18121 ttgccgggca gcatgatgtc gaggaggatc agatcgggtt ttttctgcgc taccgatttc
    18181 agaccggctt caccggattc cgcttcggtt acactgaagt tttcgcgttc cagattatag
    18241 cgcaccagtt cacggatatc ttcttcatct tcgacaatca gaatctgtgt tttcaccgcc
    18301 gccataattt tctcattcat ccagcatcag gcagatacag tgatagaccg gctggtgcag
    18361 ttcctgaact ttgaaggttt cggtcgacgg aactttgatg gcgatatccg ccgcggccgc
    18421 cagcccgccg cctttgccgc cggtaagccc gactgttttc aatccaagcg ccctggccgt
    18481 cgccacggca tggcagacat ttttcgcgtt gccggaagtg ctcagaccga tcagtacatc
    18541 gcctttctgt cccagcgcat tgacgagctg ggcaaagtta aaatccggat tgcggtcatt
    18601 ggcccacgcg ctctggaacg ccggaaacga agtaagagga atgacaggaa aggcgccttc
    18661 cagctcggcg gccagttccg gcggaagtgc cgccgaaagc ggacgtgttt tgcagaaccg
    18721 tttgagcagt tcgcccgccc agtgttcggc atccgccccg cttccgccgt taccgcaaat
    18781 caggagctta ccgcctgcat tcagactttc cttaatcgcc tttacggcgg aaagaaccgg
    18841 ttcccggcag ctttccagct ccggataacg ctcgatcagg tgggtaagat ttttcgaact
    18901 cataaaagcc tccgcatcgt tgcggcgaaa ttacctcagc acctgccgct aaacaaccga
    18961 ttttaacttt ttctgtcggt cggacggtgg cgaacaataa tcccttccac cagataaatg
    19021 acgtcttccg caatattggt ggcgtgatct ccgatccgct cgatgttccg tgaagccagc
    19081 aggatgtcaa ggtagctgct cagatgcgca ggatctttca gaatctgcgc ttcgacaaac
    19141 cgccgcacat cccggcagag atcatcaatc ctgtcatcgg atccgcaaac cgcccgggcc
    19201 agcccggcat ccatgcaaac aaaggcgtta agactgtcgt gcaccatgtc acgggtgcgc
    19261 gtggcagatt cggtcatctg ctccggaacc ggcgccgccg gaaccttgca cagcttgacc
    19321 gcgcgcttgg cgatattggt agccagatcg ccgatgcgtt ccagatcgct gttgattttc
    19381 agcaccgccg tcagaaaacg gagatccatg gcaaccggct ggtagagcgc gagtatcttg
    19441 agcgcctcct cctccacctc cacttccacc tgatcgacgt cgaaatcgcc gcggatcacc
    19501 tgattggcca gctcctcgtc acgggtcagc agcgccttca ccgcgagaat cagactttcc
    19561 tcaacctgcg cgctgagaga caggatctgt tttttcagcc gttcaacttc attcataaaa
    19621 acatgtgaca tggcatcctc cttatccaaa ccgtccggtg acataatcct gcgtttcctg
    19681 caggtgcggg ttctgaaaaa tcgtactggt cgggccgtat tcaatcagcc gtcccagata
    19741 catgaacgcg gtataatcgc tcacccgcgc ggcctgctgc atgttgtgcg tcacgattac
    19801 aatcgtatac ccgccgcgca gatcggcaat cagatcctcg attttattcg tcgcaatcgg
    19861 gtcgagcgcc gaacagggct catccatcag caggacttcc ggttcggcgg cgatggctcg
    19921 ggcgatgcaa aggcgctgct gctggccgcc ggacataccg agcgcgcttt catgcagacg
    19981 gtccttcgct tcatcccaaa gcgccgcgcc gcgcagactg cgttcgcaga ctgcgtccag
    20041 caccgacttg ttgcgctcgc cgtcgatacg cagcgaataa atcacgtttt catagatact
    20101 catcgggaac gggttgggtt tctggaaaac catccccatc cgcttgcgca gctcgatgac
    20161 atccaccgac ggcgcataaa ccggctcgcc gttcagcatc atcccgccgt taatccgcac
    20221 cgtgctgagc aggtcgttaa tccggttaat cgagcgcagc agcgtggact tcccgcagcc
    20281 ggacgggccg accagcgcag tgaccttccc tttcggaata atcatattaa tgtcaaacag
    20341 cgcctgcttg gccccgtagt gcagattaaa tccctgaaca tccagcagcg cttcttcatg
    20401 cttcaacgca tcgtgcgttt cggtcgggaa tgttttgccg gcggcaatct ccgcgaggcg
    20461 gttcgctttc tttttcggcg gactttgtac ggtcatggaa tccataggaa ttctccttaa
    20521 aatgaggcgg aaacaaattt acggcgcagg cgcgtcctta gacggacggc cagcaggttc
    20581 agcagaaaaa tcagtacgat cagcagcagc gtggtggtga agaccatcgg cttggcggct
    20641 tcgctgttct gactctggaa gccgaggtca tagatatgaa atccgagatg cataaaactg
    20701 cgctcggcat gaacgaacgg agccacgccg tccaccggca gctccggcgc cagcttcacc
    20761 gcgccgacca gcatcagcgg agccacctcg cccgcgccgc gcgccatcgc gagaatcatg
    20821 ccggtcataa tcccgggcag cgcgcgcggc agaacaatcc gtttaatcgt ctgccacttg
    20881 cttccgccgc aggcatagga accttcgcgc atcgaattcg gtacggcggc cagcgcctct
    20941 tccgtggcga caatcaccac cggaagcgtc agcagcgcca gcgtcagcga agcccagaga
    21001 atcccgccct tcccgaaggt cggattcggc agcgatgagg caaaaaacag ttcgtcaatg
    21061 gaagcaccga tcaggtagca gaaaaagccg aggccgaaca cgccgaagac aatgctgggc
    21121 acgccggcga ggttgttgat ggaaatgcgc accgcgctga cgatcagccc cggcttggcg
    21181 tattcgcgca gataaagcgc cgccatcacg ccgaacggaa ccaccgccag cgacatcagc
    21241 agcgtcataa tcaccgtgcc gaagatcgcc ggaaacacgc cgccttcgct gttggcttcg
    21301 cgcggctcat cgcacaggaa ttcatgccag cggctgaaat agattccggt cttgccggca
    21361 agcgtaagct gattggccgg ataggcgcgg acaatcgttt caagcggcat tgccaccgct
    21421 ttgccctgcg ctgttttcag attcagtaca tagcggctgt tttccgttcg cagcaggccg
    21481 atttcatcct gaagtttctt aaactccgtc gtgacggatt tttctgtttc caggaatttc
    21541 gcgtgctccg cctgccaggc cggactgctc agtccgtcgc gcagctccac cgtacggaga
    21601 tccaggcggg ccttttcaag ctgccggttg acgcggccga catcgcgtgt ttcgagcgcc
    21661 cggatttttg cgcgccggtc gcttacctcg ccatgatacc ggttaaaggc cttccaggcc
    21721 gcttccggcg tagacgccac cgcttcgccg cccagcgtaa agccttccgg aaatccgtag
    21781 aagcggcccc acgcggtacg ttcaaccacc agcgcccact ccggcagggt ttccgatttg
    21841 atttcgtgac ggttgaccca ggtgaaatgc tcgccggaga tttcatagtt tccggtgcgc
    21901 accagtttac ggtgcagaac ccggacgggc tcctcgttgc tgcggaaact ttcgtcgcgg
    21961 gtgatttcac ccatgtaaac ctggttgctc accgtttgaa tctgcggaac cggaaccggc
    22021 cagagcgtgg cgattccgtt atagaaaatg agcagcagca gaccggtgat catgcacagc
    22081 gcaaccgcca gcccgccgcc ggtcagccag atcatcggct cgccctgcgc gaaaagcgaa
    22141 accttcgcgt gacgccggcg gggcttgacg gttttttctt ctgccggttg caaactcaca
    22201 gttccaccgc ctttttacgg aagcgcatac ggatcagctc cgcgatggtg ttgaggataa
    22261 aggtcagaac aaagagaacc agcgcggcga ggaacagcgt gcggtaatgc gtgctgttgc
    22321 gcaccgcttc cggcagctcc accgcaatgt tggcggaaag cgtacggaag ccgttgaaaa
    22381 tgttccattc catcaccggc gtgttgccgg cggccatcag cacaatcatc gtttcgccga
    22441 cggcgcgtcc gaggccgatc atgatcgcgg agaacaatcc gctcatcgcc gtggggatga
    22501 tgatgcggac cgccgtctgc cacggcgtcg ccccggctcc cagcgatgcc gaacgcagat
    22561 gatccggcac cgaggaaagc gcgtcttccg aaatggtgta gatgatcggg atgatggcga
    22621 aacccatcac aaacccgacg accagcgcgt tgcgctgaac ataggttccg aagagtccgc
    22681 cgcgcggatc aaaaccaagt gactgaaaaa gtgccgcagc agccagcgcc agcagaacgg
    22741 cgaatacaaa accgaaaatg aatttgccca gatcaaacag cgcgcactgc agccgcgacc
    22801 agttcattga aaagaatctc agcctcggat cgattacgag gctgaaaagc caggcaacca
    22861 gcagacagga aaccgggatc atcagtaaca cccagccgcc gaacgccgta ccgatctgtc
    22921 cgtcgagcca gcgcatgata tcgccggcaa agcagagctt ttcgatcagc ggactgacga
    22981 tccgggccag ccccagtccg acgggaaccg ccagcagcat aaagagcaga cggaagcgtg
    23041 ccaaccgaag cgtccagtgc tgcggaataa tctgccagat atacgcaccg agcagaaacg
    23101 acgccggaac ggtgataaag accgtcatga aaactgtgag catgcctgcc acaatcggtg
    23161 ccagcaccag cgcggcgata aagccgagca caacgctcgg cagaccggcc atcatttcga
    23221 ccgcgggttt gatgtgcatc ttggcgcgcg gcgaaagaaa ttcgctcgtg aaaatggcgg
    23281 ccagcagcgc caccggtacg ccgaagagca ttgagaagag cgtcgctttg agcgttccga
    23341 aaatcagcgg catcagaccg agcttcggtt cgaagtcgtc ggtgccggac gaggactgcc
    23401 agacgtgctc cggcttcgcg gcgctttcgt accagacggg caggaaaagc gcggcgggtg
    23461 tcacttccgg atgccgcgga tcaatattcc actgcacaag atgtttcggt gtcagcgcga
    23521 gcagtccgtc atctttggga gccagcgcca gcgcctgtac cggcgcgtcg ccggcggcca
    23581 gatgcgcaag ctgtttctgg ctggtcacgt gaaacagatc gaccgtgccg tcggcatagc
    23641 ctgcggcaaa catgcggatg cgcgacgaag aagccagcgc ggtaacagcg gcggatccat
    23701 gaaacgtatg tgccgcagtc agggaagttt gcgaggagcc gtttggtgaa agcgttggga
    23761 accaggcctg gacgcgcccg gaggaatcac cggccagcag tgtggtgcgg cccagcagca
    23821 gctgaagcga tgtcagcttg cggtccggtt ccggaacaag gtcaatccgt tcgatcagcg
    23881 acggattcat aaagttccgg gtgtcaaacc gcagcagcgt accgtccttc caggcggcgc
    23941 agaccgtatc gcccagtccc gaaagaagaa cccatgccgg cggctcgcgg tggtctttgg
    24001 tgttgtaggg aatcacagcg gcagtgcggc ggaccgttac tttgccggtc ataatgtttt
    24061 cgcggcgggt gaagcgcagc agcgaaagcg ttccttcctc gttcagcatg acggaaatct
    24121 ggccggcagg actgttgatc tggtccagca gccgaaccgc cgacggcgtt tcgccgggaa
    24181 ccggctcgtc aaaaacagcc gacagtttga tggagcggaa ctgttcagcc gaaatccgct
    24241 gaatcaaggt gccgttccac tcgccggttt tagcttcctc cagcgtccga agctcaggcg
    24301 gcagatcttt tgcatcaaca tacgaggtca ggaaggtgat ccgccccatt tgaatcgagc
    24361 cgtcagcaaa accgcagagt acttcggacg tctgggcatc ctgtgtgaaa gcggtcagcg
    24421 cggcattggt gaacagcatc tgttcggaaa tgaccgcgcc ggtatcgagc cgcgtacaga
    24481 ccatccgtcc gtcggcgtat agaatgcggt tcatcactcc gtactcgtcg gattgaatcg
    24541 aaaacggctt cgcgctgtcg gcggcgggcc ggttcagcgt ttcgacgccg tcaatgcgcg
    24601 ccggacggaa gagcggaacg gtgacgtaga gcagatagaa gaagacggtg gaaacggcga
    24661 ggatagtgcc gattccgccg acggtgatca gcgattttga aaaacggtcg gacgcccgca
    24721 cactccagtg cgttttcttg cgccttgccc gtcctgtaaa actttgcgtc atccgtttcg
    24781 ccgcctccgg ttttaatctc aaaggatagc gaccgcccga ggccgctatc ctgacaaatt
    24841 atatttcggc ttagaagcct gcttttatgc cgatcgattc cagtgtttta cgggcgatgt
    24901 tggccgggac cgggtagtat ccgtctttta ccacgtccgt ctgaccctgt ttgctgaaaa
    24961 tgtatttaat gaactcgcgg cggagcggat cgagttcgct gccgggtttg cagttcacat
    25021 agagatacag gtaacgggcc aacggatatt cgccgctgta ggcgttttcg ggagtggctt
    25081 ccaccggagc ggaaaacgcg tcctgcgcca gcggaacagc gcgtacgtcg gctgttttat
    25141 agccgatgcc gctgtagccg atgccgcctt tgtcggtggc aacgccctga accacagccg
    25201 aactgccggg ctgttctttc acagaatcct tatagtcgcc tttgccgagc gcattttctt
    25261 tgaaatagcc gtaggtgccg gaagcggcgt tacgtccgta aagactgatc ggcaggtttg
    25321 cccattcgcc tttaagtccg agctgacccc agccggtgat gtcttccggg ttacccagct
    25381 tgcggttctt tgagaaaatc gcatccacct gcgtcagcga cagcgctttg atcgggttgt
    25441 ctttgtggac atagacggcc agcatgtcga tactggtcgg cagttcggtc ggcttataac
    25501 cgtatttcgc aacgaacccg tcgatctcca tctgtttcat cgggcggctc atcgggccga
    25561 aggtggccgt accttcaatc agcgccggcg gcgcggttga agaacctttg ccttcgattt
    25621 caacctgcac gttcgggtac atttttttga atccttccgc ccagagcgtc atcatgttgt
    25681 tcatcgtgtc cgagccgatt cctttgattg ttccggaaac gccctgtact gctttgtaat
    25741 ccggaaggcc ggcatccacc tgcacctgag cctgagctgt cacaaaagcc gccgcgatca
    25801 gtgccgcact taaacttatt ttcttcatgt agagtttctc cttaatttcc actctcttta
    25861 cttttctgcg gacaccggac gggaggtctt ccctttcgcc tcctcttccc ggcaaccgcc
    25921 ttttacggga agaaatatgc acggactcta ttaggacatt gttaggattt tattagaacc
    25981 gtgcaaaaac ttcaccgcgc aatggtaagt gtcttaaaaa ctggagcggt gcgcgccgga
    26041 atgctaaaca gggggtatga aattacaaca aggtcagcta tggaaaaaag acggagagtt
    26101 tttccgcatt gtcgaatgcg aacggctttc cgtcacatat aaagtgatgg caaacccttt
    26161 cgccaaagaa ggtgtgcagc atcaggtcac gaaaaaagag ttctgccggc tgatcaaaga
    26221 cgccaaactg ctgccgtagg aatcgcgaaa tggacgatgc cccgataaaa atcaagcttt
    26281 cgccgccgca gaaggcgctt ctgcgccaga agctcatcgc tatgtttctg gatgacttcg
    26341 acgaagaact cagcgacttc aaagcggatc agattctgga tacattcatc gaaaaactcg
    26401 gtcccggcat ttataacgcc gccatcgagg atatgaaaat cttcatgatg catcagctcg
    26461 aagacctcga cgccatcttc cagaagtaac cgctgcaatc cccgctattt cgcgagccgg
    26521 gttccctttt cttcaaaggt aatcagccgc tttttgtaga gtgcgccgat cgcctgcttg
    26581 taggttttct tactgacgcc gaacagacgg tagatttctt ccggcggact tttatcggtc
    26641 acaggcatga atccgccctg cgccttgatg taattcagaa tgacatccgt gagctcagtg
    26701 actttttcaa aacctggttt ctggagacaa agatcaatct taccgtcgcc ccgcacctgc
    26761 ttaatgaagc cgtccagttt ctgcccgcgt tccagcggct gaaaaacttc gttgtaaaac
    26821 aggatgcccc agtgccggcc gttaatgatg gctttaaacc ccagatccgt cttgtcgcag
    26881 accagcagcc gaaccttatc gccggtttta tagttcgacg gtgtccggtc gaggaatttg
    26941 tcgagtttgg cggatgcggc gatgcgcccg ctggtttcat cgagataaat gcgcacaaca
    27001 taggactgcc cctcgcgcat ttccactttc tgttcccgga acggcacaaa cagatccttc
    27061 ggcagtcccc agtcgaggaa cgcgccgatg cgtgtggcgg aagccacgcg cagacaggca
    27121 aactcgccaa ccatcgcaac cggcctcaat gtcgtggccg tcagccggtc ctcggaatca
    27181 atcatcacaa aaacctcgat gatgtcatcg aggtgccact ccttggaaac gtaccgtttg
    27241 ggaagcagaa tgtcgcccag actgcctccg tcaaggatca gtccgaagtc tgttgaccgc
    27301 aatacctgca gccggttgat ttgtccgatg gtcgccattc gtgttctctc tggggttaaa
    27361 gtggaacaca ccgtacttct ttttccggag gttggaaact tttcccgcag caagacacat
    27421 ttccggccct a
//
LOCUS       DAOPZO010000011        26446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_1818_length_26446_cov_7.941912, whole genome shotgun sequence.
ACCESSION   DAOPZO010000011 DAOPZO010000000
VERSION     DAOPZO010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 26446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26446
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1818_length_26446_cov_7.941912"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PKI68_01685"
     CDS             complement(<1..400)
                     /locus_tag="PKI68_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52436.1"
                     /translation="MNISGFKCMLCGAVDARPWLTNCPDYYLQKCVPVDYVECAACSL
                     IQQYPLPHDVSALYADYPVHTARSTVQRLARRIFQRQVYFRPEGGSKHLTLLDYGCGD
                     GTFLREMKSRFKTVYGFEPGDEHASSLAGQT"
     gene            complement(397..1302)
                     /locus_tag="PKI68_01690"
     CDS             complement(397..1302)
                     /locus_tag="PKI68_01690"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNX52437.1"
                     /translation="MKRVLVTGAGGFIGRQTLAPLLAAGCEVHAASLTVPDEAPPGIC
                     WYTNDLLNPLEISKLLNAVKPTHLLHLAWYAAPGKFWNAPENRDWLEAGKVLLSAFCD
                     TGGQRVVFAGTCAEYEAGHGQCVENQTPLKPATLYGICKNELHQFAVNFAQQNGRSMA
                     WGRVFHLYGPYEYPQRFVPSVILSLLRGEEARCTEGTQERDFMHVRDVASAFAALLLS
                     PVEGSVNIASGCPVRLADIARRIAAKLDAEKLLKLGALPISAGDPPVLTASTGRLNRE
                     VNWTPSIPLEQGIDESVRWWRARTL"
     gene            complement(1299..1829)
                     /gene="rfbC"
                     /locus_tag="PKI68_01695"
     CDS             complement(1299..1829)
                     /gene="rfbC"
                     /locus_tag="PKI68_01695"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNX52438.1"
                     /translation="MKFEQTAIPGVWLIDIEPHKDERGFFSRTWCAEEAAAHGLNPAC
                     VQCSISFNKMRGTLRGMHWQAEPHAEAKWIRCTRGAVWDVALDLRSASPTYGKWTAVE
                     LSADNHRTIYIPEGVAHGFQTLEADTELFYQMSEIYVPELARGARWDDPAFGIKWPVS
                     NPVLSDRDRAYEDFKL"
     gene            complement(1829..3058)
                     /locus_tag="PKI68_01700"
     CDS             complement(1829..3058)
                     /locus_tag="PKI68_01700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX52439.1"
                     /translation="MSELKCRFCSSSLHESLADLGMAPLSNAYLDAADLQCSETFYPL
                     HAFVCSKCRLAQLPALETPENIFNDYAYFSSYSDSWLEHCRKYVEQICAQRSLGAHSR
                     VIELASNDGYLLQYFKQRGIPILGVEPAANVAAEAERKGVSTLVRFFGTETAKEMLAT
                     GMCADLLVANNVLAHVPDLNDFVEGIRMVLKPEGIATLEFPHLQNLLEKRQFDTIYHE
                     HFSYFSFLTARRIFSAHGLRIFHVEELPTHGGSLRIHVCHTGSSEKETAEVAKLVQRE
                     TALGYDSPEPYALFHEQVQKVKRDLLRFLIDAREAGKTVAGYGAPAKGNTLLNYCGIR
                     EDLLAFTVDISPHKAGRFLPGTHIPIYAPDRIREVKPDYVLILPWNLKDEIVRQMADI
                     RSWGGRFVVPIPEVEIV"
     gene            complement(3303..5291)
                     /locus_tag="PKI68_01705"
     CDS             complement(3303..5291)
                     /locus_tag="PKI68_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52440.1"
                     /translation="MRSEQLKKAVIPALILGGGLFLRLFRLGTASLQIDNFLFWDLFH
                     REVSAGGIFAHWMELTGVTGQLPFAAAAAKLFIDILHLPLSFGTMILPFALCGALAIP
                     VAYMAGREFGGPRMAALLAAMVAFSPICIQMSREAYFYTPALLGAFLALWAAGLSGRM
                     LRDKPVPRGFHIVNAAAFLVMTWSSPSTWPYAFFFALFNVALFGWQLIRHRRGMSDVL
                     IVLVTYLVIGLPLLLAPWGLEQIKQFTSDGATRDYWTKVFAAGRGVSQWDKIWPVFSS
                     YAWGITPVRQIFSALIVFSGLYACIAGFRKNRTFAVLGLIFVAVLMLNVLALEKSVWI
                     FGMTRVVPLVPFYLLFLSAGLLYPFEKLRGKRGGVLTVLVPVIGFGLWIEPVSLIPSL
                     TGIPRPYAEIAAWADQHLPEDTPVLTERFFTAYNEFRVHAPKKVQFISTVPNQIPEQY
                     NGLEFRKRTLQFFKDNPGSAFYEEKHLWDRAETGPWPEVRELFARSHTVTNRAGMRLA
                     QLGLFDQGDPSVPYSPEPFTSTIYYNLPEDVAARARTEGPAAVILFSKGWKTVSTRDY
                     RTWRLLQSAAVLEAWNLTDAPQKAAVVLHGVAAGGAKQVELVGGAQKIFPNGQLTDWT
                     LGSVTLAPGKNLLMLRDPLPGNAVPLLVSSVEIRTLPF"
     gene            complement(5281..5976)
                     /locus_tag="PKI68_01710"
     CDS             complement(5281..5976)
                     /locus_tag="PKI68_01710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX52441.1"
                     /translation="MKSAFDAYGESYDDVMKKSIGFIGQKHDYYTQAKADILLDSLRR
                     QYGATRQLSVLDVGCGIGKTDGFLSPDLGKLTGVDVSSASIERARRENPDVQYEVYDG
                     RSLPFDNATFDAAFLICVLHHVAPEERVALLREVRRTVRPGGSVFIFEHNPFHPLTRL
                     AVARCEFDRDAQLLSQRAAKGLLRDAGFWLMESQYILFLPFKSKVLRGTDFLRRNVPL
                     GAQYFVTGVNHAE"
     gene            complement(5973..6914)
                     /locus_tag="PKI68_01715"
     CDS             complement(5973..6914)
                     /locus_tag="PKI68_01715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX52442.1"
                     /translation="MKTNPIVSVVVPFLNEEENLPELYSRLKAVFSGRSETAEFLFVD
                     DGSTDGSFQWLKKMSVEDPRVRTLQFSRNFGHQAAITAGLDHASGDAVVIMDADLQDP
                     PEVLPDLLAKWSEGYEVIYAVRRKRAGEGFFKKLLAAGFYRIFRSLCSVKVPVDAGDF
                     RLLDRNVVEALKAMRETHRYMRGMTSWIGFRQCAVQYDRAARHAGETKYPVWKSIKLA
                     WDGITSFSAKPLQWMMNAGLLVALVSVLFAFRIIWLKISGNGGLVSGWSSLSVLILFL
                     GGIQLVALGLLGQYISRIFDESKKRPLYVVRKDGADA"
     gene            complement(6936..9110)
                     /locus_tag="PKI68_01720"
     CDS             complement(6936..9110)
                     /locus_tag="PKI68_01720"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNX52443.1"
                     /translation="MKNKLLAFLLLLGLAACKPPETVTYSELLSGLTNIASVARLDVP
                     ATQLISSCDRTGANEDYNYFPGKTKDGQCILADLEGPGVVSRFWFTGNLSNKKFYFYF
                     DGEKKPRLEFSWNDLRKGVAPFDIEPLSVDEQNCWYTFVPVPFKKRLLITTDDAGYQY
                     GRGDKLYYQLNWQKLPAGKTVESFSLPADSAALEKVASIWRGSSFGPLPSADKEVSLT
                     AGQTVELWIGQGPAAVNAFTIDSAVRDPDVLRNVLLKIIWDGNPEPSVSVPLGDFFGS
                     VWQSWRAQSMYFGSTGSTFFCRFPMPFQKSARFVLENKSAQTVALKFGIQTGPHIEGG
                     YFHAGWRNSAATDTGVPHTVLKAAGRGRYAGCILSVVSADRSFWALESDESMLIDGKK
                     TWQGTGLEDYFNAGWYYQNVFARPLHGLPNKAPFRTVQYRLHLTEPVLFNRSFEMEFE
                     RGPNQASHAAYESVAFYYLDRPQAADSRLENRAAPVDALQPYTLMTDLWNFERFGDLQ
                     GEAEYIDRYSRQFNPPFKEMLELRKLVCGYESKQISKQQFIDGLNASTNAQAGALRKL
                     YTVPGTALVQLYCNMPSELYLDGELLLQAGDPQKPVFTAVELKKGRHVLAAVSGWQEY
                     PKWTQVAVRTADGFVLGTSGDWKHAVNPSGDWASLAYNDSSWQTLSDFDGRVKGPPEE
                     PYVWVQPDPFVNTLSQACGLRPSASWGSKKGNVVYRKVFEIK"
     gene            9272..10315
                     /locus_tag="PKI68_01725"
     CDS             9272..10315
                     /locus_tag="PKI68_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 10"
                     /protein_id="HNX52444.1"
                     /translation="MYLGFYNFYRQYNGNRMFTDPASPIGDNLMYPFVHLGQKLRSLG
                     HQAATLDMDDVSKFDAAVFLDHPTLLNPYFRQLQKMRDKKLYLFLLENPANRPDNYWA
                     FNHRAFEKVFTWNPQLVDGKKYHRFYLPNRIPPSIKIEPEEKRKFCVTIASQKYSSHP
                     QELYSERIRAIRWFEELHPEEFDLFGTLWEHPYFTGRLARLNLGLHPVYVRFPDTLRK
                     KRFPSHRGTVPSKHDVLRQYKFAICYENAVFPGYVSEKIFDALFAGCIPVYLGAPDVT
                     DMLPPEIFIDKRRFKTYDELYDYLRNMPEAEYLKYRQAIEQFLTGPGIRPFSAEHFAD
                     TILQHIVNGNVTP"
     gene            10312..11319
                     /locus_tag="PKI68_01730"
     CDS             10312..11319
                     /locus_tag="PKI68_01730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX52445.1"
                     /translation="MNNPLVSICIPTYQRPELLERAIQSCLAQTYPHFEILITDNSPD
                     NASAEIVSRLNDPRIRYRKNETNLGPYGNINRVAALATGAYIKFLMDDDLIKPRCIEL
                     MVAALEKNPSAGLAMAPMELIDENDRRIFPRFYVFRKMAYRYRYQVGDGLIDRKKILQ
                     DFLVKDYPCCVPSGVMWRRECFTRLGYFDPKADFAVDLDFCMRIAAHWDFYYIDQVLS
                     SWRLLTNCHTATLHQKGLPVEAFYYITRKCLTEEPVRKLFENKWRTLVRDSLFFCSCR
                     ALLNILAGLRAGNPKLIAATIQTIRKEDGHLLNFLRLPLFAIREIWGSLFPKNLPEPR
                     Q"
     gene            complement(11325..13691)
                     /locus_tag="PKI68_01735"
     CDS             complement(11325..13691)
                     /locus_tag="PKI68_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52446.1"
                     /translation="MLDGKTQKVSKTKIHGLLAAGFLVLLFVLLFRNFLTGSSVLMTT
                     DAVISYANKSAADVFTETFSNWDSGVLLGLPRGAATQMASFLNAFTSGVVWNNLVYGF
                     ACLAASFIFLAGFGKRLNPWAALCGALTAFWLGSNFTLIYAGHGLKPYVVLFFVCSVL
                     SAGASSWRGGILWGGFTGLMFAQQPDVALFFALFAGVYLIFRLWNRDGFKPLKWLKVL
                     VPAAVAAFLFAGGPLLSGYKLNVKDTAQVQTETAQEKWDYITQWSFPPEESVAFIAPG
                     YTGWRSGEPDGPYWGRMGRSAGWEQTRQGFMNFMLESTYIGFIPAAFALFALFSCRRS
                     PHRAEILFWGGAALVALLLAFGKFFPLYSLFYKLPVVNNIRNPNKFLQIFQIALAILT
                     AYGFDALFKRAEQKSMRQFFWCAAAASGVLVLAALSVSLHRTEGISDFTAQGWPMDAA
                     RTIVANQSRSLGYAAFMAAAAAAVFAVFTFPRFEKFLRFRNSIAAGLILLISADVLIL
                     SKHYVQPMPRSYIQANALTDFLKKNIGTQRVALLTQQSVYNIWLTYLLPYNQIPAFNF
                     AAMPRMPEDYQAFLNAGSKDPLRMWRFSSVKYLLGPAAFEKQLAGQVKKVFAYNLAAS
                     SDNEIQIIPDPNGAQAVFELLGSVPRYALLTGFEPASGEQALNRLTGNGPLLSGQIQS
                     PAEVIRYRPGRVELKTQSGAPGMLRAAERWDPDWKAEVDGRPAEVQKIDYLCQGVALT
                     AGIHKVTLTYSPSRLFVYMQCAGYLALFAAFLLNFSSWRNCRCKNQQQ"
     gene            complement(13771..14514)
                     /locus_tag="PKI68_01740"
     CDS             complement(13771..14514)
                     /locus_tag="PKI68_01740"
                     /inference="COORDINATES: protein motif:HMM:NF039190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5672 family protein"
                     /protein_id="HNX52447.1"
                     /translation="MNGHFRIEAQDVVVVIHVYRNQISEAEKKALQQCARVLSKYDIV
                     VIHPQELDISIYKTLIPQMKSMVFGSQYFGTIKGYNKLLISASFYQTFAHYQYFLMFH
                     LDAWVFEDALLEWCNKGYDYIGAPWLEEPVRGRTSALIPFTKICVNQVGNGGFCLRKI
                     STHLRIAKRLKFLTPFYKYNEDVFWSILIPMIFRSYRKPSVQEALSFAFEYKPRECYA
                     KTGGRLPFGCHAWERTDREFWNEHIHLDA"
     gene            complement(14511..15092)
                     /locus_tag="PKI68_01745"
     CDS             complement(14511..15092)
                     /locus_tag="PKI68_01745"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNX52448.1"
                     /translation="MNLAHKLNLGCGNKKIEGFINVDSRPDCTPDVLADLDKGLEPFA
                     NDSIDEIRAEHVLEHVDDLLLVLSEMYRVSKNGAVWNIFVPHYSHGFLHPFHKRGFCC
                     RTFDWFFSKTSPESYGDLDVTVERVQLNYIRDPSGILRAVAFPVNLFANMHKRFCERI
                     WCYWVGGFEEIHYTIRVNKTSCFCGASARRHES"
     gene            complement(15291..16562)
                     /locus_tag="PKI68_01750"
     CDS             complement(15291..16562)
                     /locus_tag="PKI68_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52449.1"
                     /translation="MTKIRARLATLLADDLLKHTAILFAGMMAVHVCNLVYQMAVSRA
                     LPKEEYALLAAFLGVLAIIGYPLSTLTTGISYYSSLLQQDGRPGDVKRLLRKWLLLTG
                     IPASLLGVSVVLFNGPVAGFMNLSRFAPVIIAGAVFPAMFWLPVLTGAAQGLQLFGWY
                     SSANIVGAVFRLLLGAGFVLFLHPACGWAMLGHGSGIYVSVAVLLVGLFLALRGGTAT
                     GLRLPSMRFYLLQSSLVLAAFAVLMTADVVLVNHYLPENSEFAYAATLGRLVAWFSMA
                     VAMAMFPKVASSGGTTAEHQRIFFHSFGYTALCVAASALGCFLLPRLLLHILFGIHAA
                     PDSMVQLTRLMAAAMSACALLNVTVQFLLAQRRFKALIGVVLCALLYLLSVHFFHSTA
                     RQVAVAAGAVNTIALLAGLCAVLRLKPAAQR"
     gene            16777..17121
                     /locus_tag="PKI68_01755"
     CDS             16777..17121
                     /locus_tag="PKI68_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNX52450.1"
                     /translation="MEKEQMDIYVGNLPYTATDPDLQELFEQYGKVTSARVILDRMSG
                     RSKGFGFVEMPEKSEGQAAIDALNGSDFMSRAIRVNESQPKPAGERRSSGGGGGRGFG
                     GGDRGERRESRW"
     gene            complement(17177..17944)
                     /locus_tag="PKI68_01760"
     CDS             complement(17177..17944)
                     /locus_tag="PKI68_01760"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX52451.1"
                     /translation="MKLSIVIPAHNEEHRLPPMLEAYAGYFAEKYGNEVELIVVPNFC
                     SDRTAEIARGIGARYPQVRVVEELQRVGKGGAVMLGAKSAQGGLVGFVDADGATPPAA
                     FDDLVQKISLYGCIIASRWMKGSDVSPKQPLSRRVASRCFNGLVRSLFGLKLTDTQCG
                     AKLFRREVILPVLRNLGVTNWAFDVDMLFQTKRLGFSIREIPTVWHDVAGSKIAIGHS
                     SVAMFVALVRLRMFYSPLRFMIPVFSRLAERLLRYSG"
     gene            complement(17941..19566)
                     /locus_tag="PKI68_01765"
     CDS             complement(17941..19566)
                     /locus_tag="PKI68_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52452.1"
                     /translation="MKRATWVSITILAVALSVMLGTANLFLGSLNQDEGWYLYAAKQI
                     AGCKVLYRDFMFTQGPALPYVYGIFFPVIEKLGVEGGRMITALFGLAAAGCAAWLADR
                     SVSKKHRKAAALCAFILTGVNVYQSYFTVIVKTYALCAFFLTAGFVALSYTNRRRGAT
                     AAFWGGFLLALAACTRLSAGIALPVAGLWLLWNRKKVFPLSWVSFGLGGGFILLLGLG
                     TFFLLAPENTEFALFGYHAGREPGSLMQLLALKAGFISRFVQAYFVFTTGLLLTFALK
                     RLHWPIRERGFEEMTLDSRPKLIEMMREANAPRSPFERSFISLLWFSGMLITLVHFLA
                     PFPYDDYQVIVYPLLAAALSVTLAPHCPERQQIRAVFAVLLISTAAAVSSPLNQDWLT
                     RGRDRIWWKFKETPDLALLQKTGSEIREKLGADGLLLTQDTYLAIEAGARVPHGMEMG
                     PFSYYPDMPRAQAEKLNLLNEEMLIEILMQAHAPVAAFSGYGLTIRSPQIAELAANDR
                     EKLRSALGANYKKTAEIPNFGQAFTPLEVFERK"
     gene            complement(19660..21372)
                     /locus_tag="PKI68_01770"
     CDS             complement(19660..21372)
                     /locus_tag="PKI68_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNX52453.1"
                     /translation="MQSSQLASLLQQTGLFTSEQVEALLPAVAEPGAGFTDTVVEKTG
                     VKEELFLEKLAGAMNLPFMRLAKAEIAPELLAKVPPKAVFQYNIMPVAEEQGSLRIAT
                     ANPLQSGLIDAMRLVTGGRIKLALSPASDIESAAKRLYGVGAETLDRMMQDDDRINLD
                     EEGLFKQDLSELDQEASVVKFVNQIIWEAHQNRATDIHIEPMEVDLRIRYRIDGVLHQ
                     TPVPATLKRFQSSIISRIKVMANMDIAEKRLPQDGRISLRIQGEEIDVRVSTMPTVYG
                     ESVSLRLLLRGSGMIGMDQLGMSPEDEKIIKRMITRPHGILLCTGPTGSGKSTSLYAW
                     LHTINSVDIRIMSAEDPIEYEMAGVNQVQMKPEIGLTFAHALRTFLRQDPDVIMVGEI
                     RDRDTAEIAIRAALTGHLVFSTLHTNDSAASINRLLDMGIEPFLVASAVEGIVAQRLI
                     RRLCPSCRKPLELDAAKIELLRKEGFPVEELATHTVYEPAGCDLCRGSGFKGRTGIYE
                     ILTVDDHIRPLIIDRSASSDIKREAMRHGLRTLRDDGWRKVLDGITTVEEILRVSEED
                     EEEA"
     gene            complement(21386..22159)
                     /gene="lpxA"
                     /locus_tag="PKI68_01775"
     CDS             complement(21386..22159)
                     /gene="lpxA"
                     /locus_tag="PKI68_01775"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNX52454.1"
                     /translation="MSNIHPTAIVGEGAVLGADVKLGPYSVVGSGVKIGDGTELMAHA
                     VVDGNTTIGSGCVIHPFARVGGPTQDLKFKGGAPGVIVGDRTVIRECVTINAGTHDGE
                     MTEVGSGCLLMAYSHVAHACKVGNGVIIANGTQLAGDVIIEDFAIIEGLCGVVQFRRI
                     GKMAFLGGYTKATKDVPPFMIADGLDIEVRGFNKIGMERRGVSEESRAAIKEAYRILY
                     RQNLPVAEALEKMEKELPSTPEVQHLINFIRTSKAGIVR"
     gene            complement(22166..22597)
                     /gene="fabZ"
                     /locus_tag="PKI68_01780"
     CDS             complement(22166..22597)
                     /gene="fabZ"
                     /locus_tag="PKI68_01780"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNX52455.1"
                     /translation="MSVIEANEILEILPHRYPFMLVDRILEIEDNKRIVGIKNLTFNE
                     PFFQGHFPGRPVMPGVLQMEAMAQVAGILLNKMFGGEGKISYFAAIDNARFRRPVVPG
                     DQLRMEIEIVNAKPRLSKVNAKAFVDGKLVSEADLMFSSAG"
     gene            complement(22665..23000)
                     /locus_tag="PKI68_01785"
     CDS             complement(22665..23000)
                     /locus_tag="PKI68_01785"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNX52456.1"
                     /translation="MELEKRSFKCQQCGTCCRWSGHVLLTDADIARLAAAAEIPEEQF
                     IARYTVLAVNRRQLSLADGPDGSCVLLKAGRCALYEARPAQCRDFPRGWRVASGCPAL
                     EALDKTGAV"
     gene            23133..24266
                     /locus_tag="PKI68_01790"
     CDS             23133..24266
                     /locus_tag="PKI68_01790"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX52457.1"
                     /translation="MATFVKKTKTIGLFAAAASLAVLTACSKETPEKTKQTAAPAAEA
                     VDLAQPSASLFEQPLQPNPLALTPDDVVVTVDGEKITHGEIMQGVQMNLMQMSKRVPP
                     QQLSQMYGQIYQNVTDTLIANMLLTKAAEKSSLAVSDEELAKEIATIEAGAPQGTTLK
                     DALAENKIDYNEWQADLRKQMLVRKLVDEKTANIAEATPAEIAKFYEDNADSFKVPES
                     VSASHILIAFKPEDTDATKAQKKKDIEKIRADILAGGDFAAIAGEKSDCPSSKQGGTL
                     GTFSRGQMVPEFETAAFAQEVGKVGDVVETQFGYHLIKVTDKQPAGIRPLAEVKDQLQ
                     DYLTGKKKQEALIAYIEEIKAKSKVEFKKQDLDKAAAAPEAAK"
     gene            complement(24370..24454)
                     /locus_tag="PKI68_01795"
     tRNA            complement(24370..24454)
                     /locus_tag="PKI68_01795"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24418..24420),aa:Leu,seq:cag)
     gene            24612..24989
                     /locus_tag="PKI68_01800"
     CDS             24612..24989
                     /locus_tag="PKI68_01800"
                     /inference="COORDINATES: protein motif:HMM:NF025183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HNX52458.1"
                     /translation="MQNINDKQWKALKIKLARSRQISVPDMGEPEKEYVQSRGIDILR
                     LHATDFVKKRLAPAAPKNDGRQTPLKGHPVFIAQHATGTSDRIKLEKFHGIKTGIELT
                     EKEVAYIVSVILRWIAEQIGEPV"
     gene            25074..26285
                     /gene="pcnB"
                     /locus_tag="PKI68_01805"
     CDS             25074..26285
                     /gene="pcnB"
                     /locus_tag="PKI68_01805"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HNX52459.1"
                     /translation="MEPQIIARPDHTISRKQISAAALKVLYGLKDAGYLAYLAGGGVR
                     DLLLGRNPKDFDVATNATPEEVRRVFRNCRLIGRRFRLAHVYFHDETIEVSTFRAPAP
                     PADEVQDDDNTFLAKDGLVLRDNLFGTPEEDALRRDFTVNALFYDIADFSIIDYVNGR
                     RDLADRILRVIGDPDQRFTEDPVRILRAIRFAAALGFDIEPSAREAVRRHAEKLEGTS
                     SSRLYEEIQKLLSCGKSEQVFTLCCELDVFQHLFPELGAWLNAPDGEGKTHWLRKSFI
                     QIDRWRAAGFSIDPALLFALIFGEYHEWVAAQIMKNEGLAHAEALQEATHRHIRQLDR
                     IRIPKAVTHHIAEIMSGQPRFLKTTPKNAQRMMHHRSFLDAFLYFKFAARTAGRHAAE
                     LEWWEQQRKAK"
     gene            26340..26432
                     /locus_tag="PKI68_01810"
     tRNA            26340..26432
                     /locus_tag="PKI68_01810"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26374..26376,aa:Ser,seq:gct)
BASE COUNT         6629 a         7582 c         6796 g         5439 t
ORIGIN      
        1 cggtctgtcc ggccagcgag gaggcatgtt catcgccggg ttcaaatcca taaacagttt
       61 tgaaccggct tttcatttcg cgcaaaaacg tgccgtcgcc gcagccgtaa tccagcagtg
      121 tcagatgttt cgatccgcct tccggccgga aatagacctg acgctgaaaa atgcgccggg
      181 caagtcgctg caccgtgctg cgtgctgtat gtaccggata atctgcatac agagcgctga
      241 catcgtgcgg caaaggatac tgctgtatca gtgaacatgc ggcgcactcg acatagtcaa
      301 ccggaacaca tttttgcaga taatagtcgg gacaattcgt caaccagggc cgggcgtcga
      361 ccgcaccgca caacatgcac ttgaatccgg aaatattcac agcgtgcgag ccctccacca
      421 tctaacggat tcgtctatcc cctgttccag cggaatggaa ggcgtccagt ttacttcccg
      481 gttcagccga cctgttgatg cggttaaaac cggaggatcc cccgcagaaa tcggcaatgc
      541 accgagtttc aaaagttttt ccgcatccag cttggccgcg atgcgacggg caatatccgc
      601 gagccggaca ggacatcctg aagcaatatt gacggaacct tcaaccggac tcaacagcag
      661 tgccgcgaaa gcggacgcca catcgcgcac atgcatgaag tccctctcct gagtgccttc
      721 cgtgcagcgg gcctcttcgc cgcgcaacag ggaaagaatg acggacggaa caaaccgctg
      781 cgggtattcg taaggaccgt acagatggaa cacccggccc catgccatgc tccgcccgtt
      841 ctgttgcgcg aaattgaccg cgaattgatg cagttcgttt ttacagatac cgtagagcgt
      901 ggcgggtttc agcggagtct gattttctac acattgtcca tgcccggctt catactccgc
      961 acaggttccg gcaaaaacca cgcgctgtcc gccggtatcg caaaatgcgc tcagcaaaac
     1021 tttgccggct tccagccagt cacggttctc cggcgcattc cagaattttc cgggcgcagc
     1081 ataccaggcc agatgaagta aatgagtcgg cttgacggcg ttgagcagtt tactgatttc
     1141 taaagggttc agcagatcgt ttgtgtacca gcagatgccc ggcggcgctt catccggaac
     1201 cgtaaggctg gcggcatgta cttcacagcc ggcggccagc agcggagcca gcgtctgccg
     1261 gccgataaat ccgcctgcgc ctgtaacaag cacccgcttc acagtttaaa atcctcataa
     1321 gcgcgatcac ggtcggacaa aaccgggttg ctgaccggcc atttgattcc gaacgccgga
     1381 tcgtcccagc gcgcaccgcg cgccagttca gggacataaa tctccgacat ctgataaaag
     1441 agttcggtgt ccgcttccag cgtctgaaat ccgtgggcta cgccttcagg aatatagatc
     1501 gtccggtgat tatccgcact cagttcaaca gcggtccact tgccatatgt cgggctcgca
     1561 gaccgcaggt cgagcgcaac atcccagacc gcaccgcgag tacaccgaat ccatttggct
     1621 tcggcatgcg gctcggcctg ccagtgcatg ccgcgcaatg ttccgcgcat tttattgaat
     1681 gaaatactgc attgaacaca ggccggattc agtccgtgcg cggcggcttc ttcggcgcac
     1741 catgtgcggg aaaaaaaacc gcgttcatcc ttgtgcggct caatatcaat caaccacacg
     1801 ccgggaatcg ctgtctgctc gaatttcatt acactatttc aacttcagga atcggcacaa
     1861 caaaccgtcc gccccagctg cgaatatcgg ccatctggcg gacaatttcg tctttcaggt
     1921 tccacgggag aatcagcaca taatccggct taacttcgcg gatccggtcc ggggcataga
     1981 ttggaatgtg cgtaccggga agaaaccgac cggctttatg aggactgatg tcgacggtaa
     2041 aggccagcag atcttcacga ataccacagt agttaagcag tgtatttccc ttggccggag
     2101 cgccgtagcc agccaccgtt tttccggctt cacgggcatc aatcaggaac cggagcaggt
     2161 ctcgtttcac cttctgcacc tgctcatgga agagggcata aggctccggg ctgtcatacc
     2221 ccaaagcggt ttcacgctgc acaagctttg ccacctcggc agtttccttc tcggaactgc
     2281 cggtatgaca aacatggata cgcagagatc ctccatgcgt cggcaattct tcaacgtgaa
     2341 aaatcctcag gccatgcgca ctgaaaatcc gccgggcagt taagaaagaa aaataggaga
     2401 agtgctcgtg gtatatggta tcaaactggc gcttctccag cagattctgc agatgaggga
     2461 actccaaggt agcgataccc tccggtttca gcaccatccg aataccttcc acaaaatcgt
     2521 tgagatccgg cacgtgagcc agaacattat tagccaccag cagatcggcg cacattccgg
     2581 ttgcgagcat ttcttttgcg gtctccgtgc cgaaaaaccg tacgagtgtt gaaacgcctt
     2641 ttcgctcagc ttccgcagct acgttcgcag cgggctcaac accgagaatc ggaatgccgc
     2701 gttgtttgaa atactgcaaa agatagccgt cattgctggc caactctatc acccggctat
     2761 gcgcgccaag gctgcgctgg gcacatatct gttcaacata tttccggcag tgctccaacc
     2821 agctgtctga ataagaggaa aaataggcgt aatcgttaaa aatattttcc ggggtttcaa
     2881 gcgcaggaag ctgtgcaaga cggcatttcg aacagacaaa ggcatgcaga ggataaaaag
     2941 tttcagaaca ctgcaagtcc gccgcatcca aataggcgtt ggataacggg gccatgccaa
     3001 gatccgccaa actttcatgc aacgacgatg aacaaaaacg gcattttaat tcgctcatag
     3061 gtactcctga tattttaaaa ctaccggggc ctccgtctac ctgcggcagg actccctcct
     3121 tgggtcaaaa tttcgcaaaa ctaccgcatc cccggagccg caatcctgat ccccaccgag
     3181 gcctgaccac ctccgccgga atttaggctg aaaccttact ggttacctca aaaacatcaa
     3241 atcttatgtc ataagaacaa cgccgatctg atcatcatct ctacccgggt gctattgcgg
     3301 cctcaaaacg ggagcgttct gatctcgacc gaggacacca gcagcggcac cgcgttgccc
     3361 ggcagagggt ctcgcagcat cagcagattt ttcccggggg ctaacgtcac ggagcccaga
     3421 gtccagtccg ttagttgccc gttcggaaat attttctgtg caccgccgac cagttccacc
     3481 tgcttggctc cgcccgctgc tacaccatga agcacaacgg cggctttttg cggagcatcg
     3541 gtgagattcc aagcctcgag aaccgctgcg ctctgcaaca accgccaggt gcggtaatcg
     3601 cgggttgaga ctgttttcca gccttttgag aacaaaatca ctgccgccgg gccctctgtt
     3661 cgggcacggg cggccacgtc ttcaggcaaa ttatagtaaa tggttgaggt gaacggctct
     3721 ggagaatacg gaaccgacgg gtcgccttga tcgaaaagac cgagctgtgc cagccgcatg
     3781 ccggctcgat ttgtcacggt gtgactgcgc gcaaacagct cgcgcacctc aggccacggc
     3841 ccggtttcgg cacgatccca gagatgtttc tcctcataaa acgcgctgcc cggattgtct
     3901 ttgaaaaact gaagggtgcg tttacggaat tccagtccgt tatactgctc gggaatctga
     3961 ttgggaaccg tggaaataaa ttgaaccttt ttgggtgcat gaacgcgaaa ttcattgtag
     4021 gcggtgaaaa agcgttcggt taagaccggc gtatcctcgg gcagatgttg atccgcccat
     4081 gcggcaatct ccgcataggg cctggggatt ccggtaagcg acgggatcag actgaccggc
     4141 tcgatccaca ggccaaagcc gatgacaggg accagtacgg tcaagactcc accccgctta
     4201 ccgcgcagtt tttcaaacgg atagagcagt ccggcagaca aaaaaagcag gtagaacgga
     4261 acgagcggaa cgacgcgggt catgccgaaa atccaaacac ttttttccag agcgagaacg
     4321 ttcagcatca acaccgcaac aaagatcagg ccgagcaccg caaacgtgcg gtttttcctg
     4381 aatccggcga tacaggcata cagtccggaa aaaacaatca gcgcagaaaa aatctgacgc
     4441 acgggcgtga taccccatgc ataggaactg aacaccggcc atattttatc ccactgcgaa
     4501 acaccgcgac cggcagcaaa aaccttggtc caataatcac gggttgcgcc gtctgaagta
     4561 aactgcttaa tctgctccag cccccacggc gccagcagca gcggcaggcc gatgaccaga
     4621 taggtcacca aaacaatcag cacatcggac atgccgcggc gatggcggat gagctgccag
     4681 ccgaacagcg caacgttaaa cagcgcgaag aaaaaagcgt atggccatgt gcttggcgaa
     4741 gaccaggtca taaccagaaa agcggccgca ttcacaatat gaaaccctcg cggaaccggc
     4801 ttgtctctca gcatccgtcc ggaaagtccc gcagcccata atgcaaggaa ggcgcccagt
     4861 aatgcggggg tataaaaata ggcctcacgg gacatctgga tacagatcgg actgaaggcc
     4921 accatagctg caagcagtgc agccatgcgc ggcccgccga attcacggcc cgccatataa
     4981 gcaacaggaa tcgccagcgc gccgcacagc gcaaaaggca gaatcatcgt tccgaacgaa
     5041 agcggcagat gaagaatatc gatgaacaat ttagcggcag cggcagcaaa cggaagctgt
     5101 cccgtcactc cggtcagttc catccagtgc gcaaagatcc cgccggcgga aacctcccgg
     5161 tggaagagat cccagaaaag gaaattatca atctgcaagg aagcagtccc cagccggaac
     5221 aggcgcagaa aaagtccgcc acccagaatc aatgccggta taaccgcttt tttcaattgt
     5281 tcactccgca tgatttactc ctgtaacgaa atattgcgcg cccaacggaa cgttccgccg
     5341 tagaaaatct gttccgcgca gaaccttcga cttgaaaggc agaaaaagaa tgtattgaga
     5401 ttccatcaac caaaacccgg catcccgaag gagtcccttt gcggcacgct gtgacagaag
     5461 ctgcgcatcg cgatcgaatt cacaacgggc gacagccagc cgggtgagcg gatgaaacgg
     5521 gttgtgttca aagataaaca ccgagccgcc gggccggacg gtgcggcgca cttcacgaag
     5581 caacgcgaca cgctcttccg gagcaacatg atgcagcacg cagatcaaaa aggcggcatc
     5641 aaacgttgcg ttatcaaacg gcagggaacg tccgtcatat acctcgtact ggacgtccgg
     5701 attttctcgg cgggcccgct cgatgctggc ggaggaaaca tccacgccgg tcagtttccc
     5761 caaatctgga gaaaggaacc cgtctgtttt tccgatgccg caaccgacat cgagaacact
     5821 cagttgccgg gtcgcgccat actgccgtct aagagagtcg agaaggatgt cagccttggc
     5881 ctgagtgtaa taatcgtgct tctgcccgat aaaaccgatg gattttttca tcacatcgtc
     5941 atagctctcg ccatatgcgt cgaaagctga cttcatgcat cggctccgtc tttgcgcact
     6001 acataaagcg ggcgtttttt ggattcatca aaaatacggc tgatgtactg cccaagaagt
     6061 ccaagcgcaa ccagctgaat cccgccgaga aatagaatca ggacgctgag cgacgaccag
     6121 ccgctgacca gcccgccgtt accggaaatt ttcagccata tgatgcggaa agcaaacagc
     6181 accgaaacca gagcaaccag cagtccggca ttcatcatcc actgcaatgg tttggcggaa
     6241 aatgaggtga taccgtccca cgccagtttg attgatttcc agaccggata cttggtttct
     6301 ccggcatggc gcgcggcgcg gtcgtattga acagcgcatt gccggaagcc gatccaggat
     6361 gtcatgccgc gcatataccg atgggtctcg cgcatggctt tcagcgcctc gaccacattg
     6421 cggtcgagca gtcggaagtc gcccgcatcc accggcactt tgacgctgca cagggaacgg
     6481 aagatacggt aaaaaccggc ggcgagcagt tttttgaaga agccctcgcc tgcgcgcttg
     6541 cggcggacag cataaatgac ttcataccct tcgctccatt tggcaagcag gtcaggaagc
     6601 acttcgggag gatcctgcag gtctgcatcc ataatgacaa cggcatcgcc ggaagcatgg
     6661 tcgagccccg cagtgatagc ggcctgatga ccgaagttgc gggaaaactg aagcgttcgc
     6721 acccgaggat cttcgaccga catttttttc aaccattgaa aacttccgtc ggttgagccg
     6781 tcgtccacaa ataaaaattc ggcggtttcg ctgcgcccgg agaaaacggc cttcaaccgg
     6841 ctgtaaagct ccggcaggtt ttcctcctca ttaagaaaag gaacaacaac ggaaacaatc
     6901 ggattggttt tcatggccgt tttttacctt atcacctatt taatctcgaa cactttccgg
     6961 taaacgacgt tccctttttt actgccccac gatgcagaag gacgtaatcc gcaagcctgc
     7021 gaaagcgtat tcacaaaagg atccggctga acccagacat acggctcctc cggcggcccc
     7081 ttaacgcgtc cgtcaaagtc actcagcgtc tgccaggaag agtcgttata cgccaaagag
     7141 gcccagtcgc cggacggatt caccgcatgt ttccaatcgc cggaagtgcc cagcacaaaa
     7201 ccgtcggctg tacgcacggc gacctgcgtc cacttcggat actcctgcca gccggaaacc
     7261 gcggccagta catggcggcc ttttttcagc tccaccgcag taaacaccgg cttctgcgga
     7321 tcgccggcct gcaaaaggag ctcgccgtcg aggtagagct cggacggcat gttgcagtag
     7381 agctgcacca gcgcagtgcc cggaaccgtg tagagtttcc gcagagctcc ggcctgcgca
     7441 ttggtcgatg cgttcagacc gtcaataaac tgctgctttg aaatctgttt cgactcatag
     7501 ccgcaaacca gcttgcgcaa ctccagcatt tctttgaacg gcgggttaaa ctgccggctg
     7561 taccgatcaa tgtattcggc ttcaccttga aggtcgccga accgctcaaa gttccagaga
     7621 tcggtcatca aggtgtacgg ctggagcgca tcgaccggcg cggcgcgatt ttcgaggcgg
     7681 ctgtcggcgg cctgcggccg gtccagataa taaaaagcga cactttcata cgcggcgtgg
     7741 ctggcctggt tcggcccgcg ctcaaattcc atttcaaaac tgcggttaaa cagcaccggc
     7801 tccgtcaggt gcaggcggta ctggacggta cggaaaggcg ctttattcgg cagaccgtgc
     7861 aggggacggg caaagacgtt ctggtagtac cagcccgcgt tgaaataatc ttcaaggccg
     7921 gtgccctgcc aggttttctt tccgtcgatc agcatggatt catcagactc cagcgcccag
     7981 aaactgcggt cggcgctgac aaccgaaaga atacagccgg cgtagcgtcc gcgtccggcg
     8041 gcttttaaaa cggtatgcgg cacgccggtg tcggtggcgg cactgttgcg ccaccctgca
     8101 tgaaaatatc cgccttcgat atgcgggccg gtttgaatcc cgaatttgag cgcgacggtc
     8161 tgcgcgcttt tattctcaag cacaaaccgg gcggatttct ggaacggcat cggaaagcga
     8221 cagaagaagg tgctgccggt cgagccgaag tacatggatt gcgcgcgcca gctctgccag
     8281 acactgccga aaaagtcgcc gagcggaacg ctgaccgacg gctccggatt tccgtcccag
     8341 attattttca gaaggacgtt gcgcaataca tcgggatcgc ggaccgctga gtctatcgtg
     8401 aacgcattca ccgctgccgg cccctgcccg atccaaagtt caacggtctg cccggccgtc
     8461 agagaaactt ccttatcagc ggacggaaga gggccgaaac tggagccgcg ccaaatactg
     8521 gcaacttttt ccagtgcagc agagtcagcc ggcaacgaaa agctttcaac ggttttcccg
     8581 gcaggaagtt tttgccagtt gagctgataa tataatttgt ccccgcggcc gtattgatag
     8641 ccggcatcat ccgtcgtgat gagcagccgc tttttgaacg gcacaggtac aaaggtgtac
     8701 cagcaattct gttcatcgac cgaaagcggt tcgatatcga acggcgccac acccttgcgc
     8761 aggtcgttcc aggaaaattc aagacgcggt tttttttctc catcgaaata aaaatagaat
     8821 tttttattcg agagatttcc cgtgaaccaa aaacgggaaa ccacaccggg accttcaaga
     8881 tcggcaagaa tgcactgccc gtctttggtt ttccccggaa agtaattata gtcctcattg
     8941 gcgcccgtac ggtcgcagga actgatcaac tgcgtagcgg ggacatccag ccgggcaacg
     9001 gatgctatat tggttaatcc gctcaacagc tctgaatacg tcacggtttc cggcggcttg
     9061 caggccgcca gtcccaggag aagaagaaac gccagcagtt tatttttcat catttaccag
     9121 agagctatag tgccagagga acatgcgccg ccggtgaaga cggtgccaaa accgtaaagg
     9181 aataacaagc atacgatggg aatagacgcc tgtagccgcc atccggatgc attccctttg
     9241 acattcctgc tcaaaacttt agcttagcct catgtatctg ggcttttaca atttttaccg
     9301 gcagtacaac ggcaatcgga tgtttaccga tccggcctcg cccatcggcg acaatctgat
     9361 gtatcctttt gtccatctcg gccagaaact gcgctccctt ggacatcagg cggccacctt
     9421 ggacatggat gatgtctcca agtttgatgc cgccgtattc ctggatcatc cgacgctact
     9481 gaacccctat tttcgtcagc tccaaaagat gcgggataaa aaactatatc tctttcttct
     9541 cgaaaatccc gccaaccgtc cagataacta ttgggcattc aaccatcgcg cgttcgaaaa
     9601 agtatttacc tggaaccctc aattggtaga cggcaagaag tatcaccgtt tttatctacc
     9661 caaccggatt ccgccctcca tcaaaataga accggaggag aaaagaaaat tctgcgtcac
     9721 cattgccagt caaaaataca gcagccatcc gcaggaactt tattccgagc gcatacgggc
     9781 tattcgctgg tttgaggagc tgcatcccga ggaattcgac ctgttcggca cattatggga
     9841 gcacccctat tttactggca ggctggcgag gctgaatctg ggactacatc cggtttacgt
     9901 ccgatttccg gataccttac gcaagaaacg ctttccctct catcgcggca cggttccaag
     9961 taaacacgat gtattgaggc aatataagtt cgccatctgc tatgaaaatg ccgttttccc
    10021 cggctatgtg tccgagaaaa tttttgacgc tttgtttgcc ggatgcattc ctgtctatct
    10081 cggtgcgccg gatgtgacag atatgcttcc gccggagatt ttcatcgata agcggcgatt
    10141 caaaacttac gacgagcttt atgattatct acgcaatatg ccggaggccg aatatctgaa
    10201 gtaccgtcag gcgattgaac aatttctgac aggccccggc atccgcccat ttagcgccga
    10261 acattttgca gatacgattc ttcaacacat cgtcaacgga aacgtgacgc catgaacaat
    10321 ccactggtct ccatttgtat tccgacatac cagcgcccgg agcttctgga gcgtgcgatc
    10381 cagtcctgcc tggctcaaac ttatccgcat tttgaaatcc tgattacgga caacagcccg
    10441 gataacgctt cggccgaaat agtctcccga ttaaacgacc cccggatccg ctaccgcaaa
    10501 aacgaaacca accttgggcc ttacggcaac atcaaccgag tggcggcact ggcgacagga
    10561 gcttatataa agtttctgat ggacgatgac ctgatcaagc cccgttgcat agaactgatg
    10621 gtagccgctc tggagaaaaa tccatcagca gggctcgcca tggctccgat ggagttgatc
    10681 gacgaaaatg atcgacgcat cttcccccgt ttttatgtat tccggaagat ggcgtatcgc
    10741 taccgttatc aagttggcga cggattgatc gaccgaaaaa aaattctgca ggactttctg
    10801 gtgaaggatt atccctgctg cgtgcccagc ggcgtgatgt ggcgccggga atgcttcacc
    10861 cgactgggct actttgaccc caaggcggac tttgctgtgg atctggattt ctgcatgcga
    10921 atagcggcac attgggattt ttactacatc gatcaggttc tctcgtcttg gaggttactc
    10981 accaactgtc atactgcaac cttgcaccag aaaggcctcc cggttgaggc gttctactat
    11041 atcactcgaa agtgcctgac tgaagagccg gtccggaaac ttttcgaaaa caaatggaga
    11101 acgctagtgc gtgacagtct gtttttttgc tcttgccgcg ctcttctgaa catcctggcc
    11161 ggcctgcggg cgggcaaccc gaaactaatt gcagccacaa ttcaaaccat ccgcaaagaa
    11221 gacggacatc tgctaaattt cctgcgtctg ccgctctttg ctattcgtga gatctggggt
    11281 tcactgtttc caaaaaatct gccggaaccc agacaataac aacttcactg ttgctgattt
    11341 ttacagcgac agttgcgcca gctggaaaaa ttcaagagga aggctgcgaa aagcgccaga
    11401 taacccgcgc actgcatata aacaaacagc cgcgaaggcg aataggttag cgtgactttg
    11461 tggatgccgg cagtcagtgc aacgccctga cagagatagt cgatcttctg cacctccgcc
    11521 ggacgcccgt cgacttcagc tttccagtcc ggatcccagc gttcagcagc gcgcagcatg
    11581 cccggcgcac cggattgcgt tttcaattcg acccggcccg gacggtaccg gatcacttca
    11641 gccggactct ggatctgccc gctcagcagc ggcccgttac cggttaagcg gttcagcgcc
    11701 tgctcacccg aagccggttc aaatccggtc agcaatgcgt aacgcggaac cgatccaagc
    11761 agctcaaata cggcctgcgc gccattcggg tcgggaatga tctggatttc gttgtccgaa
    11821 gaagcggcga gattgtaggc aaacactttt ttcacctgac cggccagctg tttttcaaag
    11881 gccgccgggc cgagcagata tttgaccgaa gaaaaacgcc acatccgtaa cggatctttg
    11941 gatccggcat tcagaaaggc ctgatagtct tccggcatac gcggcatcgc agcaaagttg
    12001 aaggcgggaa tctggttata tggcagaagg tacgtaagcc agatgttgta gacgctctgc
    12061 tgcgtcagca gcgcgacgcg ctgtgtaccg atatttttct tgaggaagtc cgtcagggcg
    12121 ttggcctgaa tatagctgcg cggcatcggc tggacatagt gtttcgaaag aatcagtacg
    12181 tccgcagaaa tcagaaggat cagtcctgcc gcgatcgaat tgcgaaaccg gagaaacttt
    12241 tcaaatcgcg ggaatgtgaa caccgcaaac actgctgcgg cggctgcggc catgaatgca
    12301 gcatacccaa gtgaccggct ctgattcgca acgatggttc gggcggcatc catcggccag
    12361 ccctgcgcag taaaatctga aattccttcg gttcggtgca gagagacgct caacgccgcc
    12421 agcaccagaa ctcctgatgc ggcagctgca caccagaaaa actgtcgcat ggatttctgt
    12481 tctgcgcgct tgaaaagagc atcaaatccg tatgctgtca gaatcgccag cgcgatctgg
    12541 aatatctgca gaaatttatt ggggttgcgg atattgttca ccaccggcag cttgtagaag
    12601 agcgaataga gggggaagaa tttgccgaag gccagcagca aagccaccag tgcagctccg
    12661 ccccagaata gaatctccgc gcgatgcggc gaacggcggc aggagaaaag cgcaaacagc
    12721 gcaaaggccg cgggaataaa accgatatag gtgctctcca gcatgaagtt catgaagccc
    12781 tgccgggtct gttcccagcc ggccgagcgg cccatacggc cccagtatgg accgtcgggc
    12841 tcgccgctgc gccagccggt ataaccgggg gcaataaagg caactgattc ttccggcggg
    12901 aaactccact gggtgatgta gtcccatttt tcctgagccg tttcggtttg cacctgcgcg
    12961 gtatctttaa cattcagctt atatccgctc agcagcgggc cgcccgcaaa cagaaacgct
    13021 gcaaccgccg ccggcaccag tactttcagc catttaagcg gcttaaatcc gtcacggttc
    13081 cacagacgga aaatgagata cacgccggcg aagagtgcaa aaaaaagcgc cacatccggc
    13141 tgctgcgcaa acatcagccc ggtaaagccg ccccaaagga tgccgccccg ccaggaggat
    13201 gccccggcgc tcagcacgga acagacgaag aaaagaacca catacggttt cagtccgtgc
    13261 ccggcgtaaa tcagcgtaaa gttgcttccc agccagaagg cggtcaatgc gccgcaaagc
    13321 gccgcccacg gattgagccg ttttccgaac ccggccagaa aaataaaaga ggccgccagg
    13381 caggcaaatc catagaccag attattccac accacgcccg aagtgaacgc gttcagaaag
    13441 ctggccatct gcgtcgccgc cccgcgaggc agccccagca gaacaccgga atcccagttt
    13501 gaaaaggttt cagtaaaaac gtcggcagcg cttttattgg catagctgat gaccgcatcg
    13561 gtcgtcatca gcacagacga accggtcaga aaatttctga acagcagaac aaacagcagg
    13621 acgaggaaac cggccgcgag aagcccgtga attttcgttt tggatacttt ttgagttttt
    13681 ccgtccagca tagatttttc tttcttactg cgatccgtat aattccttcg tcccatacac
    13741 gcgacacctc acaggaaaga agccctgacc ctatgcgtcg agatgtatgt gttcattcca
    13801 gaattctctg tctgtccgtt cccacgcatg gcagccgaag ggaagccggc cgcccgtttt
    13861 cgcgtagcat tcccgcggct tgtattcaaa ggcaaaggac aaagcctcct gtaccgacgg
    13921 cttccggtac gaccggaaaa tcatggggat caatatactc cagaaaacgt cttcgttgta
    13981 tttgtagaaa ggcgttaaaa atttaagccg ctttgcgatg cgcagatgcg tggatatttt
    14041 tcgcagacag aatccgccgt tgccgacctg attaacgcaa atcttggtga acggaatcaa
    14101 tgcggatgtc cgtccgcgaa ccggctcttc aagccaggga gccccgatat agtcatatcc
    14161 tttattgcac cactccagca gggcgtcttc aaaaacccag gcatccagat ggaacatcag
    14221 aaaatattgg tagtgcgcga aggtttgata aaaagaggca ctgatcagga gcttattgta
    14281 tcctttgatg gttccgaaat actgactccc gaataccatg gacttcatct gaggaatcag
    14341 cgttttgtat atggaaatat cgagctcttg cgggtgtatg accacaatgt cgtatttcga
    14401 caaaaccctg gcacactgct gaagcgcctt cttctctgct tcactgatct gatttctata
    14461 gacgtgaatt acgacgacga catcctgagc ttcaatccgg aaatggccat tcatgactcg
    14521 tgtctccgcg ccgacgcccc gcaaaagcag gaggttttgt tgacccggat cgtataatga
    14581 atttcttcga agccgcccac ccagtagcac catatccgct cacagaatcg tttatgcata
    14641 ttggcaaaaa gatttacagg aaacgccacg gcccgcaaaa ttcccgaagg atcgcggata
    14701 tagttaagct gaacccgctc tacggtcaca tccagatcgc catagctttc cgggctggtc
    14761 ttgctgaaaa accagtcgaa ggtgcggcag caaaaacccc gcttgtgaaa aggatgtaaa
    14821 aatccatgac tgtagtgcgg aacaaatata ttccaaaccg ctccgttttt ggacacccgg
    14881 tacatctcgc tcagcaccag cagcaagtcg tctacatgct caaggacatg ttccgcccgg
    14941 atttcatcaa tggaatcatt ggcaaacggt tccaagcctt tatccagatc ggccagaaca
    15001 tccggcgtac agtccggcct gctgtctacg ttgataaacc cctcaatttt cttatttcca
    15061 cagcccaaat tgagtttatg agccaaattc actggatttt cttttgccga tttacaatcc
    15121 ataagaactc acagatatac attcccggaa gacgctgttt tccccgcctg tttctctaag
    15181 atgaatcatt aagcggcgtg gagtaaatca gagaatctgc ccgcacgcat taaaaaaagc
    15241 ggctctatgc gtttacgccg gacaaacgcc ggatcttgcg ccagaaacgt ctaccgctga
    15301 gcggccggtt tcaaccgaag cacagcgcaa agtccggcaa gcagcgcaat cgtattaacc
    15361 gcaccggccg ccacggcaac ctgccgggcc gtgctgtgga aaaagtgcac ggacagcaga
    15421 tataacaggg cgcaaagcac cacgcctatc aatgctttaa accggcgctg cgccagcaga
    15481 aactgcacgg tcacattcaa cagcgcgcag gcagacatcg cagcggccat cagccgggtc
    15541 aactgcacca tcgagtccgg ggcggcgtga atcccgaaca gaatatgcag taacagtcgc
    15601 ggtaaaagaa aacacccaag cgctgaagca gccacacata gcgcggtata cccgaacgaa
    15661 tgaaagaaaa tccgctgatg ttctgccgtt gtgccgccgg acgacgccac cttgggaaac
    15721 atcgccatcg ccacggccat tgaaaaccaa gccaccagcc gccccagtgt ggcggcatag
    15781 gcaaattctg aattttccgg caaataatgg ttcacgagca ccacgtcggc tgtcatcagc
    15841 accgcaaagg ccgccagaac gagcgaactt tgaagcaggt aaaaacgcat actgggcaga
    15901 cgcaatccgg ttgctgtccc gccgcgcaaa gccagaaaca atccaaccag taacactgca
    15961 acggaaacat aaatcccgga gccatgaccg agcatggccc agccgcaggc cgggtgcaaa
    16021 aacaaaacaa accccgctcc cagcagaagc cggaacaccg ccccgactat attcgccgac
    16081 gaataccagc cgaaaagctg cagcccctgt gcggcaccgg tcagcacggg cagccaaaac
    16141 atcgccggga acacggctcc ggcaataatg accggcgcga agcggctcaa gttcataaat
    16201 cccgcaaccg gcccattgaa taaaacaacc gaaacgccca ataaagaggc cggaataccc
    16261 gtcagcaaaa gccatttccg caacagccgt ttcacatcgc cggggcgccc gtcctgctga
    16321 agcagactgc tatagtaact tattccggtt gtaagcgtag aaagcggata cccaatgatc
    16381 gccagcacgc ccagaaaagc agccagcagc gcatattctt ctttcggaag agcccggctg
    16441 accgccattt gataaaccag attacagaca tgcacagcca tcataccggc aaaaaggatc
    16501 gccgtatgct tcagcagatc atccgccaag agcgttgcaa gtctggcccg gatttttgtc
    16561 atagcgttca agttgcgtaa acatagccgg aacctccacc ttacggcagt cgaaagttaa
    16621 actttccgta tcgaaagcat tggaaaataa ctgtttcttt ttttctgtcg atgtggcatg
    16681 ttctaggtcg cgttcgtcca aagggacgtt gcatcggttc taagacctcg tgtcatgggt
    16741 cgggaattgg ttatgaaaca gagtatagta tgacacatgg agaaagaaca gatggacatc
    16801 tacgtaggaa atctgccgta cacggcaaca gaccctgacc tgcaggaact ctttgagcag
    16861 tatggcaagg tcacatcggc ccgcgtcatt ctcgaccgca tgtcgggacg ctcaaaaggc
    16921 ttcggcttcg tcgaaatgcc tgaaaagagc gaaggccagg ccgcaattga cgccctgaac
    16981 ggatccgatt tcatgagccg cgcaatccgc gttaacgaat cccagcccaa accggccggc
    17041 gaacgtcgca gcagcggcgg cggcggtgga cgcggcttcg gcggcggcga ccgtggcgaa
    17101 cgccgtgaat cccgctggta aacagcagcg attcatatcg taaaagcagc gtgccttcgg
    17161 gtgcgctgct tttttattag cccgaatacc tcagcagccg ttcggccagc cggctgaaca
    17221 ccgggatcat aaagcgcagc ggcgaataga acattcgcaa ccgaaccagc gccacaaaca
    17281 tcgctaccga agagtgtccg atcgcgattt tagaaccggc cacatcatgc caaacggttg
    17341 gaatttcacg gattgaaaaa ccaagacgct tcgtctgaaa cagcatatcc acatcaaacg
    17401 cccagttcgt cacgccgagg tttctcaata ccggaagaat cacctcgcgc cggaagagtt
    17461 tagccccgca ctgggtatct gtcagtttta gcccgaacag cgatctcacc agtccgttaa
    17521 aacagcggga cgccacgcgg cgcgaaagcg gctgtttcgg actgacatcc gaacccttca
    17581 tccagcggga agcaataata cagccgtaca ggctgatttt ctgaaccaga tcgtcaaatg
    17641 ccgcaggagg tgtcgcgcca tccgcatcca caaaaccgac caggccgccc tgcgcactct
    17701 ttgcgccaag catcaccgcc ccgcccttgc cgacacgttg cagctcttcg accacccgaa
    17761 cctgcggata acgcgcgcca ataccgcgag ctatttctgc tgtgcggtcg ctgcagaaat
    17821 tgggaactac gattaactcg acttcatttc catatttttc agcaaagtat ccggcatagg
    17881 cctccagcat cggcgggagc cggtgctctt cgttatgggc cggtatgacg atcgagagct
    17941 tcatttccgc tcaaaaacct ccagcggggt aaacgcctgt ccaaagtttg gaatctcagc
    18001 ggttttttta taattcgctc caagtgcact tcgcagtttt tcccggtcat tagcggcgag
    18061 ctccgcgatc tgcggagaac ggatcgtcag cccgtatccg ctgaaagccg caaccggggc
    18121 gtgagcctgc atcagtattt cgatgagcat ttcttcattc agcagattca gtttttcagc
    18181 ctgcgcgcgc ggcatgtccg gataatagga aaacgggccc atttccatcc catgcggcac
    18241 gcgggcgccg gcttcaatcg ccagatacgt gtcctgcgtc agaagaagac cgtccgcgcc
    18301 gagtttttcg cggatttcag aacccgtttt ctgcaaaagc gccagatccg gagtttcttt
    18361 aaacttccac cagatgcggt cccgtccgcg cgtcagccag tcctgattca gcggcgagga
    18421 aaccgcggca gcggtactga tcagcaggac agcaaataca gcgcggatct gctgtctttc
    18481 cggacagtgc ggggccagcg tcacggaaag tgccgccgcc agcagcggat aaacgattac
    18541 ctgataatcg tcatacggga acggcgccag aaaatgaacc agcgtgatca gcataccgga
    18601 gaaccagagc agagaaataa agctccgttc aaagggcgac cgcggcgcgt tggcctcgcg
    18661 catcatttcg atcagtttcg gacggctgtc gagggtcatc tcttcaaagc cgcgctcgcg
    18721 gatcggccag tgaagccgtt ttaaagcaaa ggtcagcaga aggcctgtcg taaaaacaaa
    18781 gtaagcctgc acgaagcgcg agataaaacc ggctttaagc gccagcagct gcatcagact
    18841 gccgggttca cggcccgcat ggtaaccgaa cagtgcaaac tctgtatttt caggagccag
    18901 cagaaaaaat gtgcccagac cgagaaggag aataaatccg ccgcccagac cgaatgagac
    18961 ccaggagagc ggaaaaacct ttttgcggtt ccagagcagc cagagtcccg caactggaag
    19021 cgcgatgccc gccgaaagcc gggtgcaggc cgccaaagcc agcaggaatc cgccccagaa
    19081 cgcggccgtt gcgccgcggc ggcggttggt gtagctgagc gcgacaaaac cggccgtgag
    19141 gaagaaagcg cacagggcat aggttttaac aatcactgtg aaatagctct ggtacacatt
    19201 tacgcccgtc aaaataaagg cgcacagggc tgccgctttc cggtgtttct tggaaacgga
    19261 tcgatctgcc agccaggcag cgcaacctgc cgccgccaga ccgaacagcg ccgtgatcat
    19321 ccgtccgccc tcaaccccga gtttttcaat gaccgggaag aagatgccgt aaacatacgg
    19381 gagtgccgga ccttgcgtga acataaagtc acggtaaagc actttgcatc cggcaatctg
    19441 tttcgcggcg taaaggtacc agccctcatc ctgattcaga ctgcccagaa aaagattggc
    19501 cgttccaagc ataacgctca gcgcgaccgc gagaattgta atggagaccc aagtagctcg
    19561 tttcatgatg gagcgcatca tgccctgccg caaacggttt tccgaatctt ttttgcaggg
    19621 tttgaaaact tttcagcggc ttcttccgtc cgccgcagtt taagcctctt cttcgtcctc
    19681 ttcgctgacg cggaggattt cttcgacggt ggtaatcccg tcgagaactt tccgccagcc
    19741 gtcgtcgcga agcgtacgaa gcccgtggcg catggcttcg cgtttgatgt cgctggaagc
    19801 agagcggtcg atgatgagcg gacggatatg gtcatccacc gtcagaattt cgtaaatacc
    19861 ggtacggcct ttaaacccgg agccgcggca caaatcgcac ccggccggtt cgtaaaccgt
    19921 atgggtggcg agttcttcga cgggaaaccc ttctttgcgc aacagttcta ttttggcggc
    19981 atccagctca agcggcttgc ggcaggacgg acagagccgg cgtataagac gctgggcgac
    20041 aattccttcc accgctgaag ccaccaggaa cggctcaatg cccatatcga gcaggcggtt
    20101 aatactggcg gcggagtcgt tggtgtgaag cgtgctgaaa accaggtgac cggtaagtgc
    20161 cgcgcgaata gcgatttcag ccgtgtcgcg gtcgcggatt tcccccacca taatcacatc
    20221 gggatcctgg cggagaaagg tgcgcagagc atgagcaaag gtgaggccga tttccggttt
    20281 catctgcacc tgattaacgc cggccatttc gtattcgatc gggtcttcgg ctgacataat
    20341 gcggatatcg accgagttaa tggtatgaag ccaggcgtaa agcgaagtgg acttgccgga
    20401 accggtcggg cctgtgcaaa gcagaatgcc gtgcggacgg gtgatcatcc gtttgataat
    20461 tttttcatct tcggggctca tcccgagctg atccattccg atcatgccgc ttccgcgcag
    20521 aagcagacga agactgacgc tttcgccgta aaccgtcggc atggtggaaa cacgcacgtc
    20581 gatttcttca ccctgaatac gcaggctgat gcggccgtcc tgcggaagac gcttttcagc
    20641 aatgtccata ttggccatca ccttgatacg ggaaataatg gacgactgaa accgtttaag
    20701 cgtggccggc accggagtct gatgcagaac gccgtcgata cggtagcgaa tgcgcagatc
    20761 gacttccatc ggctcgatat gaatgtcggt ggcacggttc tggtgcgctt cccagataat
    20821 ctggttcaca aatttaacga cggaagcttc ctggtcgagc tcgcttaaat cctgtttgaa
    20881 aagaccttct tcatcgaggt tgattcggtc gtcatcctgc atcatgcgat cgagagtttc
    20941 tgcaccgacg ccgtaaagcc gtttggcggc agattcaatg tcggaggcgg gactgagcgc
    21001 cagcttaatg cgtccgccgg ttacaagccg catggcgtca atcaggccgg actgcaacgg
    21061 attggcggtg gcaatacgta aagaaccctg ctcttcagca accggcataa tgttgtattg
    21121 aaaaaccgcc ttgggaggaa ctttggcaag cagttccggt gcaatctcgg ccttggcaag
    21181 ccgcatgaac ggcaggttca tggctccggc cagtttttca agaaacagct cttcctttac
    21241 accggttttt tcaaccacgg tatcggtgaa gccggctccg ggttccgcaa cagccggaag
    21301 cagcgcttcc acctgctcag aggtaaagag ccctgtctgc tgcaacaaac tggcgagctg
    21361 actgctttgc atggcggatc tccgtttacc gcacaattcc ggctttagat gtacgtataa
    21421 agttgatcag atgctgcacc tccggagtgg aaggcagttc tttctccatt ttttcaagcg
    21481 cttcggcaac cggcaggttc tggcggtaaa gaatccggta agcctcttta atggcggcgc
    21541 gcgattcttc ggaaacgccg cggcgttcca tgccgatttt gttaaacccg cgcacttcga
    21601 tatcaagtcc gtcagcaatc ataaacggcg gaacgtcttt ggtggctttc gtgtaaccgc
    21661 ccagaaatgc cattttaccg atgcggcgga actgaacgac accgcaaagt ccttcgataa
    21721 tggcgaaatc ctcaatgatg acatcgccgg ccagctgtgt tccgttagcg atgatgacgc
    21781 cgttgccgac cttgcaggca tgggcgacgt ggctgtaagc catcaacaga cagccggagc
    21841 cgacttcggt catttcgccg tcatgcgtac cggcattaat agttacgcat tcgcggataa
    21901 cggtcctgtc gccaacaata acaccgggcg cgccgccttt aaatttaaga tcctgcgtag
    21961 ggccgccgac acgggcgaac ggatggatta cgcagcccga tccgatggtt gtgttgccgt
    22021 cgacaacggc atgggccatc agctctgtgc cgtcaccgat tttcacgccg gagccgacca
    22081 cactgtacgg gcccagtttg acatccgcgc cgagcacggc gccttcgccg acaatagctg
    22141 ttggatgaat attgctcatg tttttttacc ctgcggaact gaacatcaga tcggcttcag
    22201 aaaccagctt gccgtcaaca aacgccttgg cgttcacttt agaaagacgc ggcttggcat
    22261 tgacgatttc aatttccata cgaagctggt cgccgggaac aaccggacgg cggaagcggg
    22321 cgttgtcgat ggccgcgaaa taggagattt tcccttcccc gccgaacatc ttattgagca
    22381 gaatgcctgc aacctgcgcc attgcttcca tctgtaaaac tccgggcatg accgggcggc
    22441 cgggaaaatg gccctgaaag aacggttcat tgaaggtgag gttcttgatg ccgacaatcc
    22501 gcttattgtc ttcgatctca agaatacggt caacgagcat gaacggataa cggtgcggca
    22561 ggatttccag aatttcattg gcttcaatta cggacatgcg acagatctcc tttttaaatg
    22621 gttcaggcgg cgattatctt taaaacggcg catctgttca acgcttaaac tgcgccggtt
    22681 ttatccagcg cttcgagcgc aggacagccg ctggcgaccc gccatccgcg ggggaagtcg
    22741 cggcactgag ccggacgggc ttcatacaat gcgcagcgtc cggctttgag aagcacacaa
    22801 ctgccgtccg gcccgtcagc cagactcaac tgacgccggt tgacggcgag aaccgtgtaa
    22861 cgcgcaataa actgctcttc cggaatctct gcggcggcgg ccagccgggc aatatcggca
    22921 tctgtaagca ggacgtgccc tgaccagcgg cagcaggtac cgcattgttg acatttaaat
    22981 gaacgtttct ctaattccac ggtctattct cgcaactgtt atttaaaggc gaatggattg
    23041 acacaaactc ctttaaatat aaatatgccg cactcgccgc ctatcagggt tgcgcggcaa
    23101 gaggattaac aatcagaggg agaattctga acatggccac attcgtcaag aaaacaaaaa
    23161 ctataggtct cttcgccgcc gccgcatcac tggctgtact taccgcctgc tcaaaggaaa
    23221 cgccggaaaa aacaaaacaa acagcagctc cggccgccga agccgttgac ctggctcagc
    23281 cgtctgcaag ccttttcgaa cagccgctgc agcccaatcc tttggcgctc accccggacg
    23341 atgtagttgt aaccgtcgac ggcgaaaaaa tcactcacgg cgaaatcatg cagggcgtac
    23401 agatgaacct catgcagatg agcaaacggg ttccgccgca gcagctttcg cagatgtacg
    23461 gccaaatcta tcagaatgta accgatactc taatcgccaa catgctcctt acgaaagcgg
    23521 ctgaaaagtc gagtctggcc gtgagcgacg aagaactggc caaagaaata gcaaccatcg
    23581 aagccggcgc cccgcaaggc acaacgctta aagacgctct ggccgaaaac aaaatcgact
    23641 acaacgaatg gcaggcggat ctgcgcaaac agatgctggt tcgcaaactg gtcgacgaga
    23701 aaaccgccaa tattgccgaa gccactccgg ctgaaatcgc caaattctac gaagacaatg
    23761 ccgattcatt caaggtcccg gaaagcgtat ccgccagcca tattctgatc gcctttaagc
    23821 cggaagatac cgatgcaacc aaggctcaaa agaaaaaaga tattgagaaa atacgcgctg
    23881 acatccttgc cggcggcgac ttcgcagcca ttgccggtga aaagtcggat tgccccagca
    23941 gcaaacaggg cggaacactt ggcactttct cccgcggaca aatggttccg gaatttgaaa
    24001 cagcggcatt cgctcaggaa gtcggaaaag tcggcgatgt cgttgaaacc cagttcggct
    24061 atcacctcat taaggtcaca gacaaacagc cggcaggcat tcgtccgctg gccgaagtga
    24121 aagaccagct tcaggactac ctgaccggca agaagaaaca ggaagctctg atagcttaca
    24181 tcgaagagat caaagcgaaa tccaaagttg aattcaaaaa acaggatctc gataaagccg
    24241 cagcagcacc tgaagccgca aaataaaaca acgcggcgca cttaaaacaa aaaagcgttc
    24301 cttctgcagg aacgcttttt tgttttccag gagtatcgtc atcagccgca gctccaaagg
    24361 agcaaagact ggtgcgctcg agaggactcg aacctccaca cctcgcggca caagaacctg
    24421 aatcttgcgt gtctaccaat tccaccacga gcgctttttc tgtaaaacag aagcgcgtat
    24481 taaacaggaa ctttttataa agtcaacggt ttcaaaaacg aaattcgttc tccccttgcc
    24541 agtcagacac ccgcgcccta tactttggcc cgtgctggag ctgaatttat tgaaaggagg
    24601 cggcagtcga cgtgcaaaat ataaatgaca aacagtggaa agcactcaaa ataaaactcg
    24661 cccgctccag gcagatatcc gtgccggaca tgggagagcc cgaaaaagag tacgttcagt
    24721 cgcggggaat cgacattctc agactgcacg ccactgattt cgtaaaaaaa cgcctcgcac
    24781 cggccgcccc gaagaatgac ggacgccaaa ccccgctaaa aggccaccct gtctttatcg
    24841 cccagcacgc caccggcacc agcgaccgga tcaaactcga aaaatttcac ggcatcaaaa
    24901 ccggcattga actcacggaa aaagaagtcg cttatattgt cagcgttatt ctgcgctgga
    24961 ttgccgaaca gatcggcgaa cctgtttgat tttcccaagg tttggaaaaa acttctttcc
    25021 cccgctcccc gcccccgtta cattttccgg aatcttggaa acatcgttat cgtatggaac
    25081 cgcaaattat agcccgcccc gatcacacca tcagccgcaa acaaatcagc gctgccgcac
    25141 tcaaagtcct ctacggcctc aaagacgccg gctatctcgc ctatctcgcc ggcggcggcg
    25201 tacgcgacct gctgctcggc cgcaacccga aagattttga tgtcgccacg aacgccacgc
    25261 ctgaagaagt caggcgggtt ttccgcaact gccggctcat cggccgccgg ttccggctgg
    25321 ctcacgtcta tttccacgac gaaaccatcg aagtctccac gttccgcgct cctgcgccgc
    25381 ccgccgatga agttcaggat gacgacaata cctttctcgc caaagacggc ctcgtcctgc
    25441 gtgacaacct ctttggaacc cctgaagaag acgccttgcg ccgcgacttc accgtcaacg
    25501 cccttttcta cgacatcgcc gacttctcca tcatcgacta cgttaacggc cgccgcgacc
    25561 tcgcagaccg catcctccgc gtcatcggag accccgacca gcgtttcacc gaagatcccg
    25621 tacggatttt acgcgccatc cgcttcgccg ccgcgctcgg attcgatata gagccttccg
    25681 cccgcgaagc cgttcgccgt cacgctgaaa aactcgaagg aacctcttcc tcgcgtcttt
    25741 acgaagaaat ccagaagctt ctcagctgcg ggaaatctga acaggtcttc accctctgct
    25801 gcgaactgga tgtcttccag cacctcttcc ccgaactcgg cgcctggctc aatgccccgg
    25861 acggcgaagg caaaacccac tggctccgca aaagcttcat acagattgac cgctggcgcg
    25921 ccgccggttt cagcatagac ccggcattgc tctttgccct catcttcggc gaataccatg
    25981 aatgggtggc cgcacagatt atgaaaaatg aaggactggc gcatgccgaa gccctgcagg
    26041 aagccacgca ccgccatatc cgccagctcg accgcatccg catcccgaaa gccgtcacgc
    26101 accacatcgc cgaaattatg agcggccagc cgcgcttcct gaaaaccacg ccgaaaaacg
    26161 cccagcggat gatgcaccac cgcagttttc tcgacgcctt tctctacttt aaattcgccg
    26221 cccgcaccgc cggacgccac gccgccgaac tggaatggtg ggaacagcag cgaaaagcga
    26281 aataacgcct tttgccgctt gccctgcacg gctgaatagc ttacataaaa cgccttcgcg
    26341 gagaggtggc tgagtggctt aaagcagtgg tttgctaaac cgccgtacgc cgtaaaggcg
    26401 taccgggggt tcgaatcccc ccctctccgc cactttttaa cccgtt
//
LOCUS       DAOPZO010000012        25774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_1889_length_25774_cov_8.050780, whole genome shotgun sequence.
ACCESSION   DAOPZO010000012 DAOPZO010000000
VERSION     DAOPZO010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 25774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25774
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1889_length_25774_cov_8.050780"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PKI68_01815"
     CDS             <1..199
                     /locus_tag="PKI68_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52460.1"
                     /translation="NEIKLSSCFAIQSPDIKICEAVYSFPNIENKRKKRGVEGHEPKI
                     KHSRVVGTEPSRYAPGVGNLN"
     gene            242..592
                     /locus_tag="PKI68_01820"
     CDS             242..592
                     /locus_tag="PKI68_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52461.1"
                     /translation="MDNTITASGSKMFPQRIARVSFLVRYILCMVAFGIGSAFLTASG
                     AENVPLGIRIALSLTAVVILIFALFYFARHILVARVRDTGLHTAFALLIFIPLLNFLF
                     LLALLFIPTDKFKR"
     gene            complement(741..1004)
                     /locus_tag="PKI68_01825"
     CDS             complement(741..1004)
                     /locus_tag="PKI68_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNX52462.1"
                     /translation="MAALLRVKIITPKEVAYDGTALAVTIPAEMGEMQVYQGHIPVLA
                     RVVRGTVRIEKSGAEPVCFEVDEGFFRVTPDDVSLLIDTIQPL"
     gene            complement(1007..2422)
                     /gene="atpD"
                     /locus_tag="PKI68_01830"
     CDS             complement(1007..2422)
                     /gene="atpD"
                     /locus_tag="PKI68_01830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNX52463.1"
                     /translation="MKQGKIVQVIGPVVDVEFESAADLPGIYEALIVEQTEADGKVRK
                     LVLEVQQHVGGHWVRTIAMGAAEGLQRGTPVKTTGSPITVPVGQGVMGRMMNVTGDPV
                     DERGEVRAEKRLPIHRRPPLLTEQTTKPELLTTGIKVIDLICPFLKGGKVGAFGGAGV
                     GKTVVIMELINNIAKQHGGYSVFAGVGERSREGNDLYQEMSAAGVINQSDLEKSKVAL
                     VYGQMNEPPGARMRVALTALTVAEYFRDEKFQDVLLFIDNIFRFSQAGAEVSALLGRT
                     PSAVGYQPTLSTEMGDLQERITSTRSGSITSFQAVYVPADDLTDPAPATTFAHLDATI
                     VLERSLASQGIFPAVDPLASNSRALSPEIVGEEHYNTARGVQAVLQRYKELQDIIAIL
                     GIDELSNEDKVIVGRARRIQKFLSQPFTVAEVFTGQQGRQVTIEETVRGFKEILEGQH
                     DAVPESNFYMKGGIAEAIEGI"
     gene            complement(2467..3360)
                     /gene="atpG"
                     /locus_tag="PKI68_01835"
     CDS             complement(2467..3360)
                     /gene="atpG"
                     /locus_tag="PKI68_01835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNX52464.1"
                     /translation="MANLRDIRRRIKSIRSMAKITKAMQMVSISKMKRAQHAAMFGIP
                     YADMLSDILTQVTRYVGMYQHGLMEKRNGDRKLIILVGTDRGLCGSLNTNIFREVAKY
                     DRDTTLFVTLGAKAAQFVQKTGRLLIVDFDYSDQPKLSEARMVCRFVAQLFLDDEIDS
                     ADIVFSDYVTTFKQIPLTWNFLPVGQEQEFFDQMPEALKRTKIAEAQKSVKDIGEFDF
                     EPDVNLVYESLLLRSLDYQLLQIMREVRASEHSARMVSMKQATDNARDMNNNLQLVHN
                     NLRQASITKELLEIGSASGAD"
     gene            complement(3370..4899)
                     /gene="atpA"
                     /locus_tag="PKI68_01840"
     CDS             complement(3370..4899)
                     /gene="atpA"
                     /locus_tag="PKI68_01840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNX52465.1"
                     /translation="MQSIVADIEQTLAEFHPEAHSEETGRVMEVGDGVARIEGLRNIR
                     LNEMIDFGNGITGLALNLEENSVGAVVLSDAETVREGQVVRSTGRLLEIPCGRAMLGR
                     VVDPLGRPLDGKGPIETSEVNPVERPAPGIIGRQSVSAPLQTGILAIDAIIPIGRGQR
                     ELIIGDRSTGKTTIAIDTIINQARLNHEAEIAVDTKVHPVFSVYVAIGQRQSSVAQVV
                     RALEERGALKYTVVVAATASDSAAMQYLAPFSGTAIGEWFMDHGMDALVVYDDLSKHA
                     VAYRQMSLVLRRPSGREAYPGDIFYLHSRLLERSACMSKASGGGTLTSLPIIETQTGD
                     VSAYIPTNVISITDGQIYLESDLFYQGIRPAMSVGISVSRVGSAAQTKAMKEVAGKVK
                     LELAQYYEMKEFAQFGSDLDAKTVALIEHGKRITELFKQVQYNPFPVEEEVVQLWAAQ
                     QGFFNNVPVEKTREVRAKFLEFVRMRRKELLHEIGVQKKLTPEIKKSLEEVTAEYQSL
                     A"
     gene            complement(4931..5332)
                     /locus_tag="PKI68_01845"
     CDS             complement(4931..5332)
                     /locus_tag="PKI68_01845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HNX52466.1"
                     /translation="MKITRQHRRNARLLWQAVCVNGIPDADRIRAAVRDVRRSEGRAA
                     EAVLLCFMQRLEVYIRSNQVSVISADLLSAQQQERLAGLFSGTETVKAGLSFSVDSAL
                     IGGLRVEKGYQVTDVTIARQLEILKSRLLKN"
     gene            complement(5339..5923)
                     /locus_tag="PKI68_01850"
     CDS             complement(5339..5923)
                     /locus_tag="PKI68_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52467.1"
                     /translation="MSTLFMQAGSGLFSGAAGLPLLAAIENPFPKFGVNWYDFTSQAI
                     SFLIIAWILNKFVFRTVMKTVADRRREAEEAAANNERTRRELQAVEEARREVLHKAQE
                     HADRVVSEAKANVAELLEQGKVRCELLGNEMLAKAREDARLDQARLKTELKAEIATMI
                     VNLTAKLAQMNLSDADRERLLQSAINEISAPVQD"
     gene            complement(5946..6131)
                     /locus_tag="PKI68_01855"
     CDS             complement(5946..6131)
                     /locus_tag="PKI68_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52468.1"
                     /translation="MDPSIHIGVAAAGAALGVGLIGMGAVMAVGRNPGASTKIMVQAI
                     LAIAFAEACVFYAIFLK"
     gene            complement(6152..7174)
                     /locus_tag="PKI68_01860"
     CDS             complement(6152..7174)
                     /locus_tag="PKI68_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNX52469.1"
                     /translation="MQTHALIRPFVRGFKPAGLRLCVLFVIFLFGFTGLSSAETSGSG
                     AEAVAGQSVSAEVPAGEEGGEHLSVKALEIFSVAGVPVTNSMITGWLAAFILIAVSVR
                     FRRQVQAGRHTMFTGTVEALVIFLHSFLSDIMGEKLAKKTFWLLGSFFIFILCSNWLG
                     LFPGIGSIGWGHKTDTMFKVTQPLFRGVNADLNTTMALGALFFLFWFIWAWKENGPLG
                     ILKHIFAPKGGSTGIMHFVMAVVFLAVGVLEILSIIVRPFSLALRLYGNIFAGEVMLE
                     TMLHKIPALGWLLPVPFYLMEVLVGFIQSLVFMLLSAVFIMLICGHEGAEEHPEQESA
                     PDVQQH"
     gene            complement(7179..7643)
                     /locus_tag="PKI68_01865"
     CDS             complement(7179..7643)
                     /locus_tag="PKI68_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNX52470.1"
                     /translation="MANEILKNSLDPACIKLELTASGRDEAIRELVGLIHAKHRLRNM
                     EEIVQLVIEREGKMSTSLENGIAVPHGKSNSIDGLLVAVGLKQKGIDFKSTDGQPSTI
                     IILILSSASQSGPHVRCLAEIGRLLQSEANRKKILAAKDVATVYKLMTDTGA"
     gene            complement(7758..9398)
                     /locus_tag="PKI68_01870"
     CDS             complement(7758..9398)
                     /locus_tag="PKI68_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNX52471.1"
                     /translation="MKLRINQLDLAPGYTPDRLLKAAAKALRCNPSALSDVKTIRRSI
                     DARGRSPRMTVAVEVEFSGKLPAGLRDVEVVEQSSRNAEPQNKSAIINQKSPILVVGA
                     GPAGLMAALGLAEAGLKPLLIERGEAAAPRAKRVEAFWKNGTLDVESNVLYGEGGAGL
                     FSDGKLTSRSKDRPRVRRFLEALVGCGASPDILIDAEPHIGSDVLEELVPALRSRIIE
                     LGGEVRFGARLDRLEIENGAVRGAVISGAEFRTDCCVLATGHSARDIYQMLAEQGVPL
                     ETKPFAVGVRLEIPQRRIDTAQWGGSFPALGKASFRLTRREEENARACYSFCMCPGGL
                     VISCASSEGLMTTNGMSLSKRDKPLGNAAFLVPVMPEDFQSGGTRPPGAFDERARRSR
                     STFDVLAGIEFQRKMEAAAFAAGGSDYSLPAQRLVDFLEGAAPSELPAERSCTMARPA
                     QLRGLLPEFVEHTLRSAVPSMLRSMRGVVLDEALLYASETRSSSPVRVVRGENGQSAV
                     LGLYPCGEGAGYAGGIVSSGIDGLRIAEAIIDEKLKPT"
     gene            9472..11124
                     /gene="sulP"
                     /locus_tag="PKI68_01875"
     CDS             9472..11124
                     /gene="sulP"
                     /locus_tag="PKI68_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HNX52472.1"
                     /translation="MHSRLIPQVFTCYRSGYSLKHFANDLIAGLITGIVALPLAIAFG
                     IASGVTPEQGLYTAIIAGFLISFFSGSQVQIGGPTGAFIVIIYGIVQQYGMAGLTVAT
                     LMAGVMLVLMGVCRLGSVLKYLPYPLIIGFTTGIAVIIAVTQIPAFLGMKLENIPGDF
                     IGKLEVIGSHLNLINPLSAGLGLLALLIMIIWPRITRRVPGSLIAILVTTMLVTLLNL
                     PVETIGSRFGGMPNHLPSFVFPAVSLDLMRGLFQPALTIALLAAIESLLSAVVADGMT
                     GQRHRSNMELIAQGIANIVSPLFGGIPATGAIARTATNIKNGGTTPLAGIVHAVTLLL
                     ILLFFGHYASLIPMSALAAVLLIVAYNMSEWHFFVKLFYSPRSDVLVLLTVFALTVFA
                     DLTAAIQTGVVLSALLFMRRMETVTNVGIINLDDEPVDKNDPNALTRLNVPKGVELFE
                     INGPFFFGAASKFQDEISKIRMPKVLILRMRNVPAIDATGLRALEGVVTAAHRDGTVL
                     LLSGIHKQPAAAMKKCGLVDKIGHANIHKDVARAIDHARTLV"
     gene            11134..11955
                     /locus_tag="PKI68_01880"
     CDS             11134..11955
                     /locus_tag="PKI68_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNX52473.1"
                     /translation="MNLFKRFTVWLALLCALNLNAQTPFTFAVLADVQYGDKDPWKVR
                     HYRESLSRLDECVADLNRRNPAFVIQLGDLIDGYPADPVRSAQDLDTVLSVYNRLTMP
                     RYHTVGNHCIRAGRTVLGQKLGLEKFYYDFTLPGAAGWRFVVLDGMNAGEGLLGDEQT
                     KWLCALLKQSAGRQEKVICFCHFPLAGKSALTNAQEVLRSAAETGCMAAWFAGHAHGG
                     GYLLTNGIHQVTVRGMVEASARNAYALVEIHPDRLRETGIGEEPSRELPFIKPAE"
     gene            12069..>12442
                     /locus_tag="PKI68_01885"
     CDS             12069..>12442
                     /locus_tag="PKI68_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HNX52474.1"
                     /translation="MNNDSLTIKDNTANPAPLGLLAFGMTTVLLNLHNAGLYELNAMI
                     LAMGIFYGGTAQVIAGVMEWKKNNTFGTTAFTSYGFFWLTLVALIIMPKLGLAAPASS
                     MAMACFLGLWGFFTLLMFIGTL"
     assembly_gap    12443..12542
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12543..13267)
                     /locus_tag="PKI68_01890"
     CDS             complement(<12543..13267)
                     /locus_tag="PKI68_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52475.1"
                     /translation="MKQFFLPVCLLALLLEGSGCCNFYQKKGNGPGTRAEWEAYLPVL
                     TPRQMDQIRVEAETSDVTLSFDRGAYSGYKAKLSRGFAWKVPVGPYHERSFWAPDKKK
                     GGRSLCIKMDCWGTTILPFMTGLFMAGDAEVYDYSKGDCLAYERFMRAGIIPLVAYES
                     SLMPVSEGQPMPGCPNVMFPESLCSISTSLDGVKQDEMGGCYFWEPPSLLHRPKYDRL
                     TSYYFLCGLFAFGQKNDRAYMQLA"
     gene            13412..14362
                     /locus_tag="PKI68_01895"
     CDS             13412..14362
                     /locus_tag="PKI68_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52476.1"
                     /translation="MKRLLFFSLLLMHSAVSGIEKGASRQTVLAELGEPQGTMQRDGK
                     EILIFGTGTVTLQNGAVIAADLSPEYVKKAEERALKAKEIRAEKQVELEKQKLLYPED
                     HVIQIGCAYSKTETWETLPEFIRPVAGTYQYDVYIPPGYHETGSRRCKCIILESPALW
                     DSVKERVRKEKWVVVILRDATGQQIGKAMNGNFLAAYDDAVERFRISKEYIFIAGRVP
                     AAIFATLRPVAGIILQEPDFSGFEKAGFRPDLLRKNPNLRAYVMIGDKDRARMDYQAQ
                     FIIARIPKHFIGVYEGSIAVMPQIFANQAIDWMKKEYDLP"
     gene            14379..14861
                     /locus_tag="PKI68_01900"
     CDS             14379..14861
                     /locus_tag="PKI68_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52477.1"
                     /translation="MKKIILPVILAFLAGCEYEVPLSQTPSAPANPALAGTWITTGTA
                     GKNSIVMTVKTSATDYGVTYIQDNQTNCFSGFEVRVDDLNLLQLKLLTVDEKQPQKLY
                     VFAKYDRTADSLAVYLINPDVVSSKCQTTEELRKDFALHRNNPFLFNEPLKFSKSAPQ
                     "
     gene            complement(14847..16034)
                     /locus_tag="PKI68_01905"
     CDS             complement(14847..16034)
                     /locus_tag="PKI68_01905"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNX52478.1"
                     /translation="MISSDLIIIGGGAAGMFAAAVLAEEAPELKVLVLEKGAKPLAKV
                     RVSGGGRCNLTHRCADIREFVKNYPRGGAELIGPFHRFGPAETIEWFESHGVPLVTLD
                     DGCLFPQSDTSEDVISALLKTGVNVRTNAEVAKVWSFDGGFNVKLANGEQLACRKLLV
                     ATGGGAFIEGLGHTIEPCLPSLFTFKTPDKEFTALAGIVVEGVRVSAPGLKADGALLI
                     THGGVSGPAVLRLSSFGARTLAETKYHFEMKINWLPAQSEEQVFQVLEKFRAASPRKQ
                     VGTWSPVGLSSRLWKLLVARAGVDETREWGACPVKSLRHIAAQVSGFVLPITGKNPHR
                     EEFVTCGGIRLKEVNFQTMESRRQPGLYFAGEVLDIDALTGGFNLQAAWTTGYLAAQS
                     LRG"
     gene            complement(16040..16207)
                     /locus_tag="PKI68_01910"
     CDS             complement(16040..16207)
                     /locus_tag="PKI68_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52479.1"
                     /translation="MRRFLQQPLGCLTILLLAVIVWVAIQFIFVPSAERHVQRHGHTL
                     KDYTDRLFRGR"
     assembly_gap    16319..16418
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16631..17827)
                     /locus_tag="PKI68_01915"
     CDS             complement(16631..17827)
                     /locus_tag="PKI68_01915"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HNX52480.1"
                     /translation="MGKVLIIGAGGVSGVVVHKCAQHPEVFEEIVLASRTKSKCDAIA
                     AQLKRPIKTAQVDADNVPELVALIKKEKPDLVLNVALPYQDLHIMDACLETGVNYLDT
                     ANYEPEDTAKFEYSWQWAYQDRFKQAGIMALLGSGFDPGVTSVFCTYIQKHYLDEIHT
                     IDIIDVNAGDHGYPFATNFNPEINIREVTAKGRYWENGEWKETAPLSVKHRFEVPENI
                     ATPNIYLMYHEELESLAKHIPHLKRARFWMSFGDSYLKHLEVLGNVGMTRIDEVEFNG
                     QKIVPIQFLKALLPDPASLGPRTKGKTWIGCQVTGVKNGEKKTVTVYNICDHQECYRE
                     VQSQAISYTTGVPAMIGAMMLMTGTWKGAGVFNMEQMNPDPFMEQLNLCGLPWKVVEQ
                     AVIDLG"
     gene            complement(17880..18281)
                     /locus_tag="PKI68_01920"
     CDS             complement(17880..18281)
                     /locus_tag="PKI68_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNX52481.1"
                     /translation="MHPLYEKADGLSREVISALIEVHRDKGPGLIESIYERCFLRELE
                     IRKMTAVNQRIVKIEYKGLVFEEPLRCDVLVEDCLLVELKAVEKILPVHKAQLLSYMK
                     LLDIPVGLLINFHEIKLVDGLHRLILPGANL"
     gene            complement(18332..20245)
                     /gene="speA"
                     /locus_tag="PKI68_01925"
     CDS             complement(18332..20245)
                     /gene="speA"
                     /locus_tag="PKI68_01925"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HNX52482.1"
                     /translation="MTENGKWSSIQSTELYGVDSWGAGYFSVSEKGEVAVHPDGPGGA
                     SVSLYDVAKGIQERGFDLPVLVRLSDILDARIKRLHESFSKAISEYSYRGVYRGVYPI
                     KVNQQQQVVEEICGFGARYHHGLEAGSKAELIAAISFLQDPEAYLICNGYKDEEFIDL
                     GLYALKMGIRCVFVIEMPSELDLILDRSKVLGVKPILGIRMKLSTQASGHWTESGGER
                     SVFGLNTAQVIDVVDRLRKEEMLDCLKLLHYHLGSQIPNIRDIREAVKEGCRIYTGLV
                     NEGAAMGVLDLGGGLAVDYDGSHTNFTSSCNYTTAEYCSAVIEEVMSILDETNTPHPD
                     IVTESGRATVAYYSILLFNILDVSRFETHALPEKLPDDAHELLVNLMGAYEALTAKNV
                     QECYHDAIYYRDEIHELFKRGDFTLRDRALSGQIFWHILTRIAGLIRGMRYVPDEFEH
                     LTSALADVYYGNFSLFQSLPDSWAIDQLFPIMPIHRLTEKPTQEAILADITCDCDGKI
                     DRFIDQRDVKQTLPLHTLKDNEDYILGVFLVGAYQETLGDLHNLLGDTNVVSVRIDAD
                     GQVHYAREIEGDSVADVLTYVEYEPKEMVRRVREMSENAVKTGRMSAKERRAVMDAYE
                     NGLRGYTYFEREN"
     gene            complement(20390..22297)
                     /locus_tag="PKI68_01930"
     CDS             complement(20390..22297)
                     /locus_tag="PKI68_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX52483.1"
                     /translation="MKLPTTSFFRSLAFVLPAVLTVVVSIVLFTIAGVLIAQGKTAAE
                     SEIQRNMEQKLSLTKIALESPVAERDEAAIGHFLQSLLNDRDIVFAGLTIGDPKNPEL
                     QVNKVIYGFGWVVHRDQVAESRYMQGTTLIRLPEGKAAEIRLVASNRNVWEELGQQVL
                     IIIVITMLVVISLSISTFLLTHRLIFVPVQELSAAAEEIAHGHLEKEVPVSRLNEIGG
                     LAQQMDAMRQSLKKLIGDLDRSRSRLEQRVEERTRDLLEAKEAAEVANRAKSEFLANM
                     SHELRTPMNGVIGMTDILLETELKDDQKHAAETIQSSAQSLLTILNDILDFSKIEAGR
                     LELEKRTFSFQYVIETVRKIFMPDALSKGLRLEFHYPEDVPSRLVGDEVRIRQVLTNL
                     VGNAIKFTERGGVFVDVKKIAQSVTAVTLQISVRDTGIGIAPEQQKVVFEKFTQADLS
                     TTRKFGGTGLGLSISKQLIEIMGGRIGVHSEPGAGSTFYFVVTMPLSNECGAERDPAV
                     PSRRQIKARVLLAEDNQVNQLVARKILTSLGIEVEVVENGLLALEKIQAETFDAVMLD
                     CQMPVMDGYTAAREIRRLGGSFEKLPIIAMTAHAMTGDREKCIESGMNEYISKPVKKE
                     AVVEVLSRLLA"
     gene            22414..23445
                     /gene="purM"
                     /locus_tag="PKI68_01935"
     CDS             22414..23445
                     /gene="purM"
                     /locus_tag="PKI68_01935"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNX52484.1"
                     /translation="MAAKKSAYAQAGVDIDVMMNSLQRIKKDVKSTSTKGVMSEIGSF
                     GGLFQSPGKEFLLVASADGVGTKLKVAHMAGKHTTVGQDLVNHCVNDILVQGATPLFF
                     LDYLGTAALKGKVFEDVISGFCKACRENGCALLGGETAEMPGLYPPGEYDLVGTIVGQ
                     VEKKKLITGKTIRKGDVIIGLPSTGLQTNGYSLARKVIFEKAGKKLSDLVPGTKLTFE
                     KVLLAIHKSYLKPVVALMNKVQVRGMAHITGGGLVDNVPRVLPKTVDAVFDRSTWKTP
                     AIYEFIEKEGDVDHEEMYRVFNMGIGFVLFVRPDDANTALAAIRKAGEKAIIIGRVEK
                     GTGISRLIN"
     assembly_gap    23528..23627
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23739..>25774
                     /locus_tag="PKI68_01940"
     CDS             23739..>25774
                     /locus_tag="PKI68_01940"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52485.1"
                     /translation="MKKMIFAALSLVTMLAHGQNDEWNITTKKTEKYTGIVLSNGQIG
                     ILPSEHVFGIKHIILNNTYDLDPKTEVSRIVQGMNFGNLEMEIDGEKVTDSNIENWSQ
                     TLDLKEAKLTTSFTFKDKAAVSYTIYALRNLPNAGYIDVKVGAKNKIAVHVTGKISTP
                     TADYQKPKNTYKVLKDGKTSMPILQTVALSSRKNHTISTSATFVWHAINSSRESERPK
                     LLHSIPSEQENCLSFEKEIDKDTSLEFAWTAAECTDKNFADPKSESERIIINNLLTSR
                     DILIQQHKNLWSKLWEGDIEIQGDLQAQQDVRLALYHLYSFGRGDNDLSIAPMGLSVQ
                     TPYNGHIFWDSELWMFPPLLMFNQDIAKSLVNYRSDRLDAAKRKAKNYGYKGAMYPWE
                     SDDTGEEATPPFALTGTFEHHITADIAIAFWNYYRVTQDKLWLKEKGFPVLKEIADFW
                     VSRAVKNDDGSYSIKNVIGANEFAANIDDNAFTNGSAITALNYAIQAAAELAVAADPE
                     WKNVADKIIIRRFKDGTTLEHSKYAGARIKQADVNLLSYPLSIVNDRETMLKDLKYYE
                     PKLDKNGPAMGKSIFAVIYARLGDADNAYRLFNESYIPNKHPPFGALSETATANNSYF
                     ATGAGGLLQTVLFGFGGLHITDNGIVQEKPCLPKAWEKLTIKGIGPEKKTFEITR"
BASE COUNT         5807 a         7476 c         6594 g         5597 t
ORIGIN      
        1 caacgaaatt aaactgtcat cctgcttcgc catacagtct cctgacatca aaatttgtga
       61 ggctgtatat tctttcccca atatcgaaaa caagcggaaa aaacgaggag ttgaagggca
      121 cgaaccgaaa ataaaacatt cccgggttgt cggcactgaa ccatcgcgct acgctcccgg
      181 cgttggtaat ttaaactgaa ataaggctaa taacactgtt cgacaacaaa ggagatgaca
      241 aatggacaat acgattaccg catcaggaag taagatgttc ccacagcgca tagcacgcgt
      301 ttcattcctc gtgcgatata ttctgtgtat ggtcgctttc ggcattggat ccgctttcct
      361 gacagcaagc ggagcggaga atgtgccgtt gggtataagg atcgcgttgt cgctcacggc
      421 ggtcgtcatc ttgatttttg ctctttttta cttcgcccgc catatcctgg tagcacgcgt
      481 tcgagacacc ggactgcaca cggctttcgc gttgttgatt ttcatcccgc tcttgaactt
      541 tctcttttta ttggcgcttc tttttattcc gacagacaag ttcaaaaggt agatataaaa
      601 gttgaaccaa tcattgaagc gaacgcgggc taacgcccgc gccgcttagt tctttcgttc
      661 agcctaattc tattctttcc accaccgcct caacgatgcg gaggccgtcg atgctgacgg
      721 cattagattg aatagaaacc ttacagcggc tggatggtgt cgatcagcag gctgacgtcg
      781 tcgggcgtga cgcggaagaa tccttcgtcg acttcaaagc agaccggttc cgcgccggat
      841 ttttcaatac ggacggttcc gcggacgacg cgggccagca ccgggatgtg tccctgatag
      901 acctgcattt cgcccatctc ggccggtatg gtaacggcca gagcggttcc gtcgtaggcg
      961 acttctttcg gcgtgatgat tttaacccgc agcagtgcag ccatggtcag atcccttcga
     1021 tcgcttccgc gatgccgccc ttcatataga aattggattc cggcaccgca tcgtgctgcc
     1081 cttccagaat ttccttaaat ccgcgcacgg tttcctcgat ggtaacctgc cggccctgct
     1141 ggccggtgaa gacttcggca accgtaaacg gctggctcaa aaacttctga atgcggcggg
     1201 cgcggccgac gatgactttg tcttcattgg aaagctcgtc gatccccaga atggcgataa
     1261 tgtcctgaag ctctttgtag cgctggagta cggcctgaac gccgcgtgcc gtgttgtaat
     1321 gttcttcgcc gacgatttcc ggcgaaagcg cccgcgagtt ggaagccagc ggatcaacgg
     1381 cagggaaaat cccctgcgat gccagcgagc gttccagcac gatggtggcg tccaggtggg
     1441 cgaacgtcgt ggccggcgcc gggtcggtca agtcgtcggc aggcacatag acggcctgga
     1501 acgatgtgat cgatccagaa cgcgtggagg tgatgcgttc ctgcaggtca cccatttccg
     1561 tggaaagcgt aggctggtaa ccgaccgcac tgggcgtccg gcccagcaga gccgatactt
     1621 cagcgccggc ctgcgagaag cggaagatgt tgtcgatgaa gagcagtacg tcctggaatt
     1681 tttcatcgcg gaaatattcg gccaccgtca gcgcagtcag cgccacgcgc atacgggcgc
     1741 ccggcggctc gttcatctgt ccgtaaacca gagccacttt ggatttttcc agatccgact
     1801 gattgataac gccggcggcg gacatttcct gatagaggtc gtttccttcg cgcgaacgtt
     1861 cgccgacacc ggcaaaaacc gaataaccgc cgtgctgttt ggcgatattg ttgatcagct
     1921 ccatgataac aacggtcttg ccgacgcctg caccgccgaa tgcgccgact ttaccgccct
     1981 tcaaaaaggg gcagatcagg tcgattactt taataccggt cgtcagcagt tccggcttgg
     2041 tggtctgttc cgtcagcaaa ggcggacggc ggtgaatcgg cagccgtttt tccgctctta
     2101 cttcaccgcg ttcgtcaacc ggatcgccgg ttacattcat catgcggccc atgacgccct
     2161 gcccgaccgg aactgtaatc ggcgagccgg tggttttaac cggcgtgccg cgctgaagcc
     2221 cttccgccgc gcccatggcg atcgtgcgca cccagtgtcc gccgacgtgc tgctgcactt
     2281 ccagaaccag cttgcgcacc ttgccgtcgg cttccgtctg ctccacgatc agtgcttcat
     2341 aaattcccgg aagatcggcg gcgctttcaa actcaacgtc caccaccggt ccgattacct
     2401 gtacgatttt cccctgcttc atttcccgcc tcgtttcctg caattgctgc gttttaaatt
     2461 ctgcaatcag tccgctccgg acgcactgcc tatttccaga agttccttcg tgatggacgc
     2521 ctgccgcagg ttgttgtgaa ccagctgcag attgttattc atatctctgg cattgtccgt
     2581 cgcctgtttc atcgacacca tgcgcgcact gtgttcgctg gcacggactt cgcgcataat
     2641 ctgcagcagc tgataatcca gactgcgcag aagcagcgat tcatagacca ggttcacatc
     2701 cggctcgaaa tcgaattccc cgatatcttt gacgctcttc tgcgcttcgg caattttcgt
     2761 ccgcttcagc gcttcgggca tctggtcgaa aaattcctgt tcctgaccca ccggcagaaa
     2821 gttccacgtc agcggaatct gtttaaaggt tgtgacatag tcgctgaaaa caatatccgc
     2881 gctgtcgatt tcgtcatcca gaaaaagctg ggctacgaac cggcagacca tgcgggcttc
     2941 ggaaagtttc ggctgatcgc tgtagtcgaa gtccacgatc agcagccgtc cggttttctg
     3001 tacaaactgc gcggctttgg cgccgagggt cacaaacagc gtggtgtcgc ggtcatactt
     3061 ggcgacttca cggaaaatat tggtattcag cgagccgcac agtccgcgat ccgtgccgac
     3121 cagaatgatc agtttgcggt cgccgttccg cttttccatc agcccgtgct gatacatacc
     3181 cacataacgt gtcacctgag tcagaatatc gctcagcata tcggcgtagg gaattccgaa
     3241 catcgccgcg tgctgggcgc gcttcatctt cgaaatcgac accatctgca tggccttggt
     3301 tattttggcc atggagcgga tcgacttaat gcgccgtcgg atgtcgcgta aatttgccat
     3361 agatatccgt taagccagac tttgatattc ggccgtcacc tcttcgaggc tcttcttgat
     3421 ttccggcgtc agttttttct gaactccgat ttcatgcagc agctctttcc gccgcatccg
     3481 gacgaattcg agaaacttgg cgcggacttc acgcgttttc tcaaccggta cattgttaaa
     3541 gaatccctgc tgggccgccc acagctgaac aacttcctct tccaccggaa agggattgta
     3601 ttgaacctgt ttaaacagct cggtaatacg tttgccgtgt tcaatcagcg cgacggtctt
     3661 cgcatccaga tcggaaccga actgggcgaa ttccttcatt tcataatact gcgccagttc
     3721 cagtttcact ttaccggcaa cctctttcat cgccttggtc tgcgcggccg atccgacgcg
     3781 cgagacggaa atcccgaccg acatcgccgg acggataccc tgatagaaca gatcgctttc
     3841 cagataaatc tggccgtcgg tgatggaaat cacgttggtc ggaatgtagg cggaaacgtc
     3901 gccggtctgc gtttcaatga tcggcagcga agtcagcgtg cctccgccgc ttgctttcga
     3961 catacaggcc gaacgctcca gcagacggct gtggagataa aaaatgtcgc ccggataggc
     4021 ttcgcggccg gacggacggc gcagcaccag cgacatctgc cggtaagcca ccgcgtgctt
     4081 ggaaaggtcg tcatacacca ccagcgcatc catgccgtga tccataaacc attcgccgat
     4141 ggccgtgccg ctgaacgggg ccagatactg catggccgcg gagtccgatg cggtggccgc
     4201 cacgacgacg gtatatttca atgcaccgcg ctcttccagc gcacgcacaa cctgcgccac
     4261 gctcgactgc cgctgtccga tcgcgacata aacagaaaat acaggatgaa ccttcgtatc
     4321 cacggcgatc tcggcttcgt ggttcagacg cgcctggttg atgatcgtgt cgatcgcaat
     4381 ggtggtcttg ccggttgagc ggtcgccgat aatcagttcg cgctgtccgc ggccgatcgg
     4441 aatgatcgcg tcaatcgcga gaataccggt ctgcagcgga gcggaaaccg actgccgtcc
     4501 gataatgccg ggagccgggc gttccaccgg attcacctcg gaagtttcaa tcgggccttt
     4561 gccgtccagc ggacgtccga gcgggtcgac gacgcggccg agcatggcgc gcccgcaggg
     4621 aatctcaagc agccgtccgg tggagcgtac gacctgtccc tcgcgcaccg tttcggcgtc
     4681 gctcagcact accgcgccca cactgttctc ttcaaggttc agtgcgagcc cggtgatacc
     4741 gttgccgaag tcgatcattt cgttcagccg gatattgcgc aatccttcga tccgggccac
     4801 gccgtcgccg acctccatga ctctgcccgt ttcttcgcta tgcgcctccg gatgaaattc
     4861 cgccagagtc tgttcgatgt ccgctacaat agattgcata gtatgttaac cagcctttcc
     4921 gtccgaaaat tcaatttttc aacagtctgc tcttcagtat ttccaattgc cgggcaatcg
     4981 tcacatctgt cacctgatac cctttttcca cccggagccc gccgataagg gccgaatcga
     5041 cagaaaaact gagcccggcc tttaccgtct ccgttccgct aaacagtccg gcaagacgct
     5101 cctgctgctg agccgacagc aggtccgcac tgatcacact gacctgattt gaacggatat
     5161 aaacctccag ccgctgcata aagcacagca gcacggcctc ggctgcgcgg ccttcgctcc
     5221 gccgcacatc ccggacagcc gcccggatgc ggtccgcatc gggaatgccg ttaacacaca
     5281 cggcctgcca aaggaggcgg gcgttccggc ggtgctgtcg ggtgattttc atgcgcgttt
     5341 aatcctgcac cggcgcggaa atttcgttga tagccgactg cagcaaccgc tcccggtcag
     5401 cgtcgctaag attcatttgc gccagtttcg cggtaagatt cacaatcatc gtcgcgatct
     5461 cggctttgag ttctgttttc agccgcgcct gatcaagccg agcatcttca cgcgccttgg
     5521 ccagcatttc attgcccagc agttcgcacc ggactttccc ctgttccagc agttcggcca
     5581 cattggcttt ggcttcggaa acaacccggt cggcatgctc ctgcgcttta tgcagaactt
     5641 ctctgcgggc ttcttccacc gcctgaagtt cgcgtcgagt ccgctcgttg ttggcagccg
     5701 cctcctccgc ttctctccgc cggtccgcca cggtcttcat tacggttctg aaaacaaatt
     5761 tattcaaaat ccaggcgatg atcagaaacg aaatagcctg cgaggtgaag tcgtaccagt
     5821 tgacaccgaa tttcggaaac gggttttcga tggctgccag caggggcagc cctgccgccc
     5881 cgctgaacaa cccgcttccg gcctgcatga acagtgtgct cataatgctc cgtcagccac
     5941 gtggcttatt tcaggaaaat ggcgtaaaat acacaagctt cggcgaaagc aattgccaga
     6001 atcgcttgaa ccatgatttt cgtgctcgcg ccgggattcc ggcctactgc cattacagcg
     6061 cccatgccga taagccctac gccaagcgcc gcgccggcgg ccgcaacacc gatatgaatt
     6121 gatggatcca tttttttatc tcctattcag gttaatgttg ttgtacgtcc ggagccgact
     6181 cctgctccgg atgctcctct gcgccttcat ggccgcaaat cagcataata aaaacagcgg
     6241 aaagcagcat aaaaaccagc gactggataa aaccgaccag cacctccatc agataaaacg
     6301 gaaccggcag cagccagcca agcgcaggaa tcttatgcag catggtctcc agcattactt
     6361 ctccggcaaa aatattaccg tacaaccgta acgccagcga aaagggacga acgataatgg
     6421 acaggatttc cagcaccccg acagccagga ataccactgc catcacaaaa tgcatgatac
     6481 ccgtcgagcc gcctttgggt gcaaaaatgt gttttaaaat accgagaggc ccgttttctt
     6541 tccacgccca gatgaaccag aagaggaaaa acagcgcgcc cagcgccatc gttgtattga
     6601 ggtcggcatt cactccgcgg aacagcggtt gcgtaacttt gaacatcgta tcggttttat
     6661 gcccccagcc aatacttccg atccccggaa ataatcccag ccagttggag cagagaataa
     6721 aaataaaaaa agaacccagc agccagaagg ttttctttgc cagcttctcg cccatgatat
     6781 ccgacagaaa actgtgcagg aaaatcacca gcgcttcgac ggttccggta aacattgtat
     6841 ggcggccggc ctgcacctgc cggcggaacc gtaccgaaac cgcaatcaga ataaatgcgg
     6901 ccagccatcc ggtgatcatt gaattggtaa ccggcacgcc ggcgaccgaa aaaatctcga
     6961 gagctttgac ggacagatgc tctccgccct cttcgccggc cggaacctcc gcggaaacag
     7021 actgcccggc aaccgcttcc gcgccgcttc cggaggtttc cgctgaagac aaaccggtaa
     7081 atccaaacag aaatatcaca aacaaaacac acaaccggag ccccgccggt ttaaacccgc
     7141 gtacaaaagg ccggatgagt gcgtgtgttt gcataatatt aagcgcctgt gtcggtcata
     7201 agcttataaa cggtcgctac gtcctttgcg gccagaattt ttttgcggtt agcctctgac
     7261 tgcaacagcc gcccgatttc ggccagacag cgtacatgcg ggccggattg cgatgccgaa
     7321 gaaagaatca aaatgatgat cgttgaaggc tgaccgtccg tgcttttaaa atcaatcccc
     7381 ttctgcttca gcccgaccgc caccagaagc ccgtcaatcg aattggattt cccgtgcgga
     7441 acggcaatgc cgttctccag gctggtactc atctttccct cacgctcgat caccagctgc
     7501 acaatctcct ccatgttgcg caaccgatgt ttggcatgaa tcaggccgac cagctcacgg
     7561 atagcctcgt cccgcccgga tgcagtcagt tccagcttta tacaggcggg gtcgagactg
     7621 ttctttaaaa tctcattggc catattcatc ctctcggaac aaatgtcact taaacgccgg
     7681 aactataagc cttgcgctgt ttttccgcta aaaaaaacag taaaccagac tggctttata
     7741 cgaaccggtc tggtgcgtca agttggtttt aatttttcat caataattgc ttcagcgata
     7801 cgcaggccgt cgatgccgga gctgacaata ccgccggcgt agccggcgcc ttcgccgcag
     7861 ggatagagcc caagaacagc ggactgcccg ttttcgccgc gcacaacgcg caccggactg
     7921 gaactgcggg tttcggaagc gtagagcaac gcttcatcca gaaccacgcc gcgcatactt
     7981 cgcagcatac tcggcaccgc gctccgcaac gtgtgctcaa caaactccgg caggagtccg
     8041 cgcaactgcg ccggacgcgc cattgtacag gaacgttccg ccggaagctc cgacggcgcg
     8101 gcaccttcca gaaaatcaac cagccgctga gcaggaagac tgtaatccga tccgcctgcc
     8161 gcgaacgccg ccgcttccat tttccgctga aactcaatgc ctgccagcac atcaaaggtg
     8221 gagcgcgatc tccgagcacg ctcatcaaac gcgcccggag gtcgcgttcc accactttgg
     8281 aaatcttcag gcatcaccgg aaccaaaaag gccgcgttgc cgagcggttt gtcgcgtttc
     8341 gacagactca tcccgttggt cgtcatcaat ccttccgacg acgcgcagga aataaccagt
     8401 ccgccggggc acatgcagaa cgagtagcag gcgcgggcat tttcctcttc gcggcgcgtc
     8461 agccggaagc tcgctttgcc cagcgccgga aacgaaccgc cccactgcgc ggtatcgatg
     8521 cgccgctgag gaatctcaag ccggacgccg acagcgaacg gcttggtttc gagcggcaca
     8581 ccctgctccg ccagcatttg gtagatgtcg cgggcgctgt ggccggtcgc cagcacacag
     8641 cagtcagtcc ggaattctgc gccggagatc accgcgccgc gcaccgcacc gttttcgatt
     8701 tcgagccggt caagccgggc gccgaagcgg acttcgccgc caagttcgat aatccggctg
     8761 cgcagcgccg gaaccagttc ttcgagtacg tcgctgccga tgtgcggctc ggcatcaatc
     8821 agaatatccg gtgacgcacc gcaaccgacc agcgcttcca gaaagcgccg gacgcgcggc
     8881 cggtctttac tgcgcgaggt cagtttgcca tcggaaaaaa gtcctgcgcc gccttcgccg
     8941 tagagcacat tgctttcaac atccagcgtc ccgtttttcc agaaggcttc aacccgtttg
     9001 gctcgcggcg cggcggcttc gccgcgttca atcagcagcg gtttcagtcc ggcttccgcc
     9061 agaccgagcg ccgccatcag tccggcaggg ccggcgccga caacgagaat tggcgatttt
     9121 tgattgatga ttgccgattt attttgaggc tcggcattgc gggaactttg ttccactacc
     9181 tccacatcgc gcagaccggc gggaagtttg ccggagaatt cgacttcgac cgcgacagtc
     9241 atacgcggcg aacggccccg ggcgtcaatc gagcggcgga ttgtttttac gtcgctcaag
     9301 gccgacgggt tgcaacgcaa ggctttggcc gcggccttca gaagccggtc aggcgtgtat
     9361 ccgggtgcaa gatcgagctg gttaatgcgt aatttcatac atgaattttc ttttattcag
     9421 gactgaaagg tatatgagag acgcttttta ttgcgaccaa aaaggagagt tatgcattcg
     9481 cgtctgatcc cgcaggtctt cacctgctac cgttcgggct attcactgaa acatttcgcc
     9541 aacgatctga tcgccggact catcaccggc atcgttgcgc ttccgctggc cattgcgttc
     9601 ggtatcgcat ccggggttac tcctgaacag ggactttaca ccgccatcat cgcaggtttt
     9661 ctgatttctt tcttcagcgg aagccaggta cagatcggag gtccgaccgg cgcgtttatt
     9721 gtgattatct acggcatcgt tcagcaatac ggcatggcgg gactcactgt cgccacacta
     9781 atggcgggcg ttatgctcgt tctgatgggc gtctgtcgcc tgggttcggt tcttaaatat
     9841 ctgccctacc cgctcatcat cggcttcacc accggcattg cggttatcat tgccgtcaca
     9901 caaatcccgg catttcttgg catgaagctg gaaaatattc ccggcgactt catcggcaaa
     9961 cttgaagtga tcggctcaca tctcaatttg atcaacccgc tctctgccgg tcttggcctg
    10021 ctcgctctgc tgattatgat tatctggccg cgcattaccc ggcgggttcc cggctcgctg
    10081 atcgcgatcc ttgtcaccac catgctggtt acgctgctca accttccggt tgaaaccatc
    10141 ggcagccgct ttggcggaat gccaaaccat ctgccttctt tcgtttttcc agccgtcagc
    10201 ctcgatctga tgcgcggact ttttcaaccg gcactcacta tcgcgctgct ggccgccatt
    10261 gaatcactgc tctcagcagt cgtcgccgac gggatgaccg gtcagcgtca ccgttctaat
    10321 atggagctga tcgcacaggg cattgccaac attgtctcgc cgctgttcgg cggaattcct
    10381 gccaccggcg ctatcgcccg cacagccacc aacattaaaa acggcggcac caccccgctg
    10441 gccggaattg ttcacgccgt caccctgctg ctgatcctgc tcttcttcgg acactacgcc
    10501 tcgctcattc ccatgagcgc gctggcagca gtcctgctga tcgtcgccta caacatgagt
    10561 gaatggcact ttttcgtaaa actattctac agtccgcgca gcgatgttct cgttcttctc
    10621 accgtgtttg cactgactgt cttcgccgac ctcacagctg ccattcaaac cggcgtggtt
    10681 ctctccgcgc tgctctttat gcgccgcatg gaaaccgtaa caaatgtcgg catcatcaat
    10741 ctcgatgatg aacccgtcga caaaaacgac ccgaatgccc tgacgcgcct gaatgtaccg
    10801 aaaggtgtcg agctgttcga aattaacgga ccctttttct tcggcgccgc cagtaaattc
    10861 caggatgaaa taagcaaaat ccgcatgccg aaagtgctca tcctgcgcat gcggaacgtt
    10921 ccggccattg acgccaccgg cctgcgcgcg cttgaaggtg ttgttacagc ggcacaccgc
    10981 gacggcaccg tcctgctgct ttccggaatt cacaaacagc cggccgccgc tatgaaaaag
    11041 tgcggtctgg ttgacaaaat cggccacgca aacattcaca aagatgtcgc ccgggccatt
    11101 gatcatgccc gcactctggt gtaaaaacac ccaatgaatc tattcaaacg cttcaccgta
    11161 tggctggctc tcctgtgcgc gctcaacctg aatgcgcaga ccccgtttac ttttgcggtg
    11221 ctggccgacg tgcagtatgg cgataaggat ccgtggaaag tgcggcacta ccgcgagtcg
    11281 ctgtcgcgac tggacgaatg tgttgccgat ctcaaccgcc ggaacccggc cttcgtcatt
    11341 cagctgggcg acctcatcga cggctatccc gccgatccgg tccggtctgc gcaggatttg
    11401 gacaccgttc tgtctgtata caaccgcctg accatgcccc gctaccatac cgtaggcaac
    11461 cattgcatac gggccggcag aacggtcctc ggccagaaac tcggccttga aaaattctat
    11521 tatgatttca ccctgcccgg cgccgccggc tggcgtttcg ttgtactcga cggcatgaac
    11581 gccggcgaag gcctgctggg ggatgaacaa accaaatggc tgtgtgccct gctcaaacag
    11641 tcggccgggc ggcaggaaaa agtgatctgc ttctgtcatt tcccgctggc aggcaagtcg
    11701 gcgctgacca atgcgcagga agtcctccgt tccgccgcgg aaaccggctg catggcggcc
    11761 tggttcgcgg ggcacgccca tggcggcgga taccttttga ccaacggaat ccatcaggtg
    11821 accgtccgcg gaatggttga agcgtcagcc cgcaacgcct atgcgctggt cgaaattcat
    11881 ccggaccggc tgcgggaaac cggcatcggc gaagaaccct cccgtgaact gccgttcatc
    11941 aagccggcgg aataggctcc gcctgcgcaa aattttcaaa aagggatgtt aattcgccgg
    12001 caaagcggta ttaaatcacg gcgttttttt cccgaaccat gaattgaata acagaaagag
    12061 atttccccat gaacaacgat tccctgacga tcaaagacaa caccgccaac ccggcaccgc
    12121 tcggcctgct ggctttcggt atgacaaccg ttctgctgaa cctccacaac gccggcctct
    12181 atgaactcaa tgccatgatt ctcgccatgg gcattttcta cggcggaacc gcgcaggtca
    12241 tcgccggcgt catggaatgg aaaaagaaca acaccttcgg aaccaccgca ttcacctcct
    12301 acggcttttt ctggctgacg ctcgtcgcac tgatcattat gccgaaactt ggcctggccg
    12361 cacccgcttc gagcatggca atggcctgct ttctcggcct gtggggattc ttcactctgc
    12421 tgatgttcat tgggacgctg aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nngcgagctg catataggcc cggtcatttt tctgtccgaa ggcaaaaaga ccgcagagga
    12601 aatagtatga ggtcagccgg tcatatttcg gccggtggag aagagatggc ggctcccaga
    12661 agtagcagcc gcccatctcg tcctgcttta ccccgtccag cgaagttgaa atgctgcaca
    12721 gcgattccgg gaacatgacg ttggggcatc cgggcatcgg ctgcccttca gaaacgggca
    12781 tcagactgga ctcgtatgca acaagaggaa taataccggc ccgcatgaac cgctcatacg
    12841 caaggcagtc gcccttggag taatcataga cttcggcatc gccggccata aaaagaccgg
    12901 tcataaacgg aagaatcgtg gttccccagc aatccatttt gatgcacaga ctgcgtccgc
    12961 ctttcttttt atccggagcc cagaaagagc gctcatgata cggcccgacc ggaactttcc
    13021 atgcaaaccc gcggctgagc ttggccttgt agccgctgta ggctccgcgg tcaaacgaca
    13081 gcgtcacatc gctggtctcg gcctccaccc ggatttgatc catttgccgg ggagtcaaaa
    13141 ccggcagata ggcctcccat tccgcccggg tcccggggcc gttgcctttc ttctgataaa
    13201 aattacagca gccgcttccc tcaagaagca gcgcaagcaa acagaccgga agaaaaaact
    13261 gtttcatgaa gctttcattc attcaaaacc gctccgaata tagctccggc ttgtatgcga
    13321 tcttctgcgg gaagtcaact gctgcggggc accggcgcac agaatctctc ttccattgta
    13381 ttccgcagtt ccgtccggta gactccggca catgaaacgg cttctgtttt tcagtctcct
    13441 gcttatgcat tctgccgtca gcggcatcga aaaaggcgcc tcccgccaaa ccgttcttgc
    13501 cgaactgggc gaaccgcaag gcaccatgca gcgcgacgga aaagaaattc tgatattcgg
    13561 aaccggcacc gtcaccctgc agaacggcgc agtgatcgcc gcggatcttt cgccggaata
    13621 tgtgaagaaa gccgaagaac gcgcattaaa agccaaagaa atccgggcgg aaaaacaggt
    13681 tgaactggaa aaacagaagc tgctttatcc ggaagatcac gtcatacaga tcggatgcgc
    13741 ctacagcaaa accgaaacct gggaaaccct gcccgaattc atccgccccg tcgcgggaac
    13801 ataccagtat gatgtttaca ttccgccggg ctatcacgaa accggcagcc gccgctgcaa
    13861 atgcatcata ctggaatcgc ctgcgctctg ggacagcgta aaagaacgcg tccggaaaga
    13921 gaaatgggtt gttgtcatcc tgcgcgacgc cacaggccag cagatcggga aagcgatgaa
    13981 cggaaacttt ctcgccgcgt atgacgacgc agtcgaacgg ttccggatat ccaaagaata
    14041 catcttcatc gccggaagag tcccggcggc gatctttgcc accctgcgcc ccgttgcggg
    14101 catcattctc caggagcccg actttagcgg ctttgaaaaa gcaggcttcc gacccgatct
    14161 tctccggaaa aatccgaacc tgcgcgccta tgtgatgatc ggggacaaag atcgggcccg
    14221 catggattat caggcgcaat tcatcatcgc ccggatcccg aaacatttca tcggagttta
    14281 tgaaggaagc atcgcggtta tgccgcaaat cttcgccaat caggccattg actggatgaa
    14341 aaaagaatat gacctgccgt aaacagccgg agataaaaat gaaaaaaatt atactgcctg
    14401 taattctggc gtttctcgcc ggctgtgaat atgaagttcc gctgagtcaa acgccctccg
    14461 caccggcaaa tccggcgctg gccgggacat ggatcaccac cggaactgcc ggaaaaaatt
    14521 ccatcgtgat gacggtcaaa acctccgcaa cggattacgg cgtcacgtac atacaggaca
    14581 accaaacaaa ctgtttttcc ggattcgaag tccgcgtcga cgacctgaat ctgcttcagt
    14641 taaaacttct gaccgtcgac gaaaaacagc cgcagaagct gtatgttttc gcaaaatacg
    14701 accgaaccgc cgacagcctt gcggtctatc tgatcaaccc ggatgtcgta tcttcaaaat
    14761 gccagaccac ggaagagctg cggaaagatt tcgcccttca ccggaacaat ccgtttctgt
    14821 ttaatgagcc gctgaaattc agcaaatcag ccccgcagtg attgcgcggc cagatagccg
    14881 gtcgtccagg ccgcctgcag attgaacccg ccggtcagcg catcgatgtc gagcacctcg
    14941 cccgcaaaat aaagccccgg ctgacggcgg ctttccatcg tctgaaaatt cacctctttc
    15001 agccggatgc cgccgcaggt gacaaactcc tcgcggtgcg gatttttccc ggtgatcggc
    15061 agaacaaaac cgctcacctg cgccgcaata tgccgcagcg acttcaccgg acacgcgccc
    15121 cactcgcggg tttcatccac cccggcgcgg gccaccagca gcttccacag ccgcgacgaa
    15181 agaccgaccg gcgaccacgt gccgacctgc ttgcgcggcg acgcggcgcg gaatttttcc
    15241 agcacctgaa aaacctgctc ctcgctctgc gccggcagcc agttgatctt catttcaaag
    15301 tgatacttcg tttccgccag cgtccgcgcg ccgaacgacg aaagccgcag aaccgccggg
    15361 ccgctcacgc cgccgtgcgt aatcagcagc gcgccgtcgg ccttcagccc cggcgcgctg
    15421 acgcgaacgc cttccaccac aatgcctgcc agcgcggtaa actccttgtc cggcgtcttg
    15481 aacgtgaaca gcgacggcag acacggctca atcgtatggc cgaggccttc gataaacgcg
    15541 ccaccgcccg tcgccaccag cagtttccga caggcgagct gctcgccgtt cgccagcttg
    15601 acgttgaagc cgccgtcaaa actccatacc ttggccactt cggcgttcgt ccggacgttg
    15661 acgccggttt tgagcagcgc gctgatgaca tcttcggatg tgtcggactg cgggaacaga
    15721 cagccgtcgt cgagcgtcac cagcggaacg ccgtgagact cgaaccactc gatcgtttcg
    15781 gccgggccga agcggtgaaa cgggccgatc agttccgctc cgccgcgcgg atagttcttc
    15841 acgaactcgc ggatatcggc gcaccgatgg gtcagattgc agcgtccgcc gccggaaacg
    15901 cgcaccttcg ccagcggctt tgcgcctttt tccagcacaa gcaccttcag ctccggcgct
    15961 tcttcggcca gcacggccgc ggcaaacatg cccgccgcgc cgccgccgat aatgatgagg
    16021 tctgatgaaa tcattttact taacgtccac gaaacagccg gtcggtataa tccttcagcg
    16081 tgtggccatg ccgctgaaca tgccgctccg ccgacggcac aaaaataaac tgtatcgcca
    16141 cccagacaat gaccgccagc agcaggatcg tcagacagcc cagcggttgt tgtaagaacc
    16201 ttctcatggc cgggggttat accacagaac acacagaaaa cacgtaaatg attcagacaa
    16261 ggccgccggg acggcggcga tacgtatcgc aggcgtcccg cctgccttgt aaacagacnn
    16321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg ccgggacggc ggcgatacgt
    16441 atcgcaggcg tcccgcctgc cttgtaaaca gactattctg ttttcaccaa tccggagcct
    16501 cgtgcaaatc gaaggtggag cgggcgctcc gcgcacgctt tgaacgcgcc gggacggcgc
    16561 gttccaccaa aacaaaccaa taaaaaacgc cccggagtcc ggggcgtttg cgttgtgatc
    16621 tttccggaaa ttatccgaga tcgatcaccg cctgctcgac cactttccac ggcaggccgc
    16681 agaggttgag ctgctccatg aacggatcgg gattcatctg ttccatattg aacacgcccg
    16741 cgcctttcca ggtgccggtc atcagcatca tggcgccgat catggccggc acgccggtgg
    16801 tgtaggaaat cgcctgcgac tgcacttcgc ggtagcactc ctgatggtcg cagatgttgt
    16861 agacggtgac ggttttcttt tcgccgttct tcacgccggt cacctggcag ccgatccagg
    16921 ttttgccttt ggtgcgcgga ccgaggctgg ccggatccgg cagcagtgct ttaaggaact
    16981 gaatcggaac aatcttctgg ccgttgaatt ccacttcgtc gatgcgggtc atgccgacgt
    17041 tgccgagcac ttcgaggtgc ttgaggtagc tgtcgccgaa gctcatccag aagcgggcgc
    17101 gcttcagatg cgggatgtgt ttggccagcg attcgagctc ttcgtgatac atcaggtaga
    17161 tgttcggcgt ggcaatgttt tcaggcactt cgaagcggtg cttgacggac agcggcgcgg
    17221 tttctttcca ttcgccgttt tcccagtagc ggcctttggc ggtcacttcg cggatgttga
    17281 tttccgggtt gaagttcgtg gcgaaaggat agccgtggtc gccggcgttg acgtcgatga
    17341 tgtcgatcgt gtgaatttcg tcgaggtaat gcttctgaat gtaggtgcag aaaacgctgg
    17401 tgacgccggg gtcgaagccg gaaccgagca gcgccatgat gcccgcctgt ttgaagcggt
    17461 cctgataggc ccactgccag ctgtattcaa acttggcggt gtcttccggc tcatagttgg
    17521 cggtatcgag atagttgaca ccggtctcga gacaggcatc catgatgtgc aggtcctgat
    17581 acggcagagc gacgttgagc acgaggtcgg gcttttcctt tttgatcagc gcgacgagtt
    17641 ccggcacatt gtcggcatcg acctgcgcgg ttttgatcgg acgcttcaac tgcgcggcga
    17701 tggcgtcgca cttcgacttg gtgcggctgg ccagcacgat ttcttcaaac acttccggat
    17761 gctgcgcgca tttgtgaacc accacaccgc tgacgccccc ggccccgata atcagaactt
    17821 ttcccatctc taattcctct tactaatatt ttttacagaa ggtaacaaag gaaacgaagc
    17881 tacaggttcg ctcccggcaa aatcaaccga tgaagtccat cgaccagttt tatttcatga
    17941 aaattgatca gcaatcccac cggaatatcc aatagtttca tgtaactcag caactgcgct
    18001 ttatgcaccg gaagaatttt ttcaacggcc ttcaactcca ccagcagaca gtcttccacc
    18061 aaaacgtcac agcgaagcgg ttcctcgaag accaatcctt tgtattcgat cttaacaatc
    18121 cgttgattaa cagccgtcat cttccggatt tcgagttccc tcagaaaaca tctttcatag
    18181 atcgactcaa tcagccccgg acctttatcc cggtgaacct caattagggc ggagatgacc
    18241 tcgcggctca atccatctgc tttttcatac aatggatgca cagacatctc ctcagaccgg
    18301 gcttcgtttt cttggttgcc ttctgtgaaa tctagttttc ccgctcaaag taggtgtaac
    18361 cgcgcaggcc gttttcgtag gcgtccatga cggcgcgccg ttctttggcg ctcatccgcc
    18421 cggtcttgac cgcgttctca gacatttcac gcacccgccg gaccatttcc ttcggttcat
    18481 attccacata ggtgagcacg tcggccaccg agtcgccttc gatttcgcgg gcgtaatgga
    18541 cctgcccgtc ggcgtcgatg cggacgctga cgacgttggt gtcgccgagc agattgtgca
    18601 gatcgccgag cgtttcctga taggctccga ccaggaagac gccgagaatg tagtcttcat
    18661 tgtctttgag cgtatgcagc ggcagtgtct gtttgacgtc gcgctggtcg ataaagcggt
    18721 cgatcttgcc gtcgcagtcg caggtaatat cggcgagaat cgcctcctgc gtcggttttt
    18781 cggtgagccg gtggatcggc atgatcggga agagctggtc gatcgcccac gagtcgggca
    18841 gcgactggaa caggctgaag ttgccgtaat agacgtccgc cagcgcgctg gtcaggtgct
    18901 cgaattcgtc gggcacatac cgcatgccgc ggatcagccc ggcgatccgg gtgagaatgt
    18961 gccagaaaat ctgcccggac agagcgcggt cgcgcagcgt gaagtcgccg cgcttgaaga
    19021 gctcgtgaat ttcgtcccgg taatagatgg cgtcgtggta gcactcctgc acgtttttgg
    19081 ccgtaagcgc ttcgtaggct cccatcaggt tgaccagcag ttcgtgggca tcatcaggaa
    19141 gtttttcggg cagcgcatgg gtttcgaacc ggctgacatc gagaatgttg aaaagcagga
    19201 tggagtaata ggccacggtc gcgcggcccg attcggtgac aatgtcggga tgcggcgtat
    19261 tggtttcatc cagaatgctc atcacctctt caatgacggc agagcagtac tcggccgtgg
    19321 tgtagttgca gctgctggtg aagttggtgt gcgaaccgtc gtaatccacc gccagaccgc
    19381 cgccgaggtc gagcacgccc atcgccgcgc cttcgttgac cagcccggta taaatgcggc
    19441 agccctcttt gaccgcctcg cggatgtcgc ggatgttggg aatctgcgag ccgaggtggt
    19501 agtgcagcag cttcaggcaa tccagcatct cttccttgcg gaggcggtca acgacatcaa
    19561 tcacctgtgc ggtgttgagg ccgaatacac tgcgctcgcc gccggattcg gtccagtgac
    19621 cgctggcctg cgtggaaagt ttcatgcgga tgccgagaat cggcttcacg ccgagcactt
    19681 tggagcggtc gagaatcaga tcgagctcgg agggcatctc gatcacgaat acacagcgga
    19741 tgcccatctt gagcgcatag aggcccagat cgataaattc ctcgtccttg tagccgttgc
    19801 aaatcagata ggcctcggga tcctgcagaa acgatatggc ggcaatcagc tcggccttgc
    19861 tgcccgcttc cagtccgtga tgatagcgcg cgccgaatcc gcagatttcc tcaaccacct
    19921 gctgctgctg gttgaccttg atcggataaa cgccgcggta aacgccgcga tagctgtatt
    19981 cagaaatcgc tttggagaag ctttcgtgga gccgtttgat gcgcgcatcc agaatgtcgc
    20041 tcaggcgcac cagtaccggc agatcgaagc cgcgctcctg aattcccttg gccacgtcat
    20101 acaaactgac agaagcgccg ccggggccgt ccgggtgaac cgccacctcg cctttttccg
    20161 agacactgaa ataaccggcg ccccagctgt cgacgccgta aagctccgtt gactgaatgg
    20221 aagaccactt gccgttttct gtcactctcg ttctcctcag attaaaaagc ggggacgttt
    20281 tacgtcctgc gccccccgtt ggaaagcgca tttttccaaa cagcgggaaa attctcagac
    20341 agccgttcaa tcttcggctt tattcagaac cggaagcctt taaaaaggtt caggccagaa
    20401 gccgggaaag cacctcgacg accgcctctt ttttgaccgg cttggagatg tactcattca
    20461 tcccggattc aatgcacttt tcgcggtcac cggtcatggc gtgtgcggtc atggcaatga
    20521 tgggaagctt ttcaaaagaa ccgccgagcc tgcggatttc gcgggcggcg gtgtagccgt
    20581 ccataaccgg catctggcag tcgagcatca ccgcgtcgaa ggtttcggcc tggatttttt
    20641 cgagtgccag caggccgttt tcgacaacct ctacttcgat tccgaggctg gtcagtatct
    20701 tgcgggccac cagctgattg acctgattat cttcggccag cagaacgcgt gctttgatct
    20761 gccgtctgga tggaacagca gggtcgcgtt ccgcaccaca ttcgttcgac agcggcatgg
    20821 tcaccacaaa atagaaggtc gatccggcac ccggttcgct gtgcactcca atccgtccgc
    20881 ccatgatttc aatcagctgt ttggaaatag aaagaccgag tccggtaccg ccgaacttcc
    20941 gcgtggtcga aaggtcggcc tgcgtaaact tttcgaaaac gactttttgc tgctccggcg
    21001 caataccgat gcccgtatca cgcacactga tctgcagcgt caccgcggtt accgactggg
    21061 cgattttttt aacatccaca aaaacgccgc cccgttctgt gaatttgatg gcgttaccga
    21121 ccagattggt gagcacctgc cggatacgga cttcgtcacc gaccagacgg gacggcacat
    21181 cctccggata atggaattcc agcctgagcc ctttggaaag cgcatcaggc atgaaaatct
    21241 tccgcaccgt ttcgatcaca tactggaaag aaaacgtacg cttctccagc tccagccggc
    21301 ccgcttcgat cttggaaaaa tcgaggatgt cattaaggat cgtcagcagc gactgcgcgg
    21361 aagactgaat ggtctctgcg gcgtgcttct ggtcgtcctt aagctccgtt tccagcagaa
    21421 tatccgtcat accgatgacg ccgttcatcg gcgtgcgcag ttcgtggctc atattggcga
    21481 gaaactcgct cttggcgcgg ttggccactt ccgcggcttc cttggcttcc agcaggtcgc
    21541 gcgtacgctc ttcaacccgc tgctccaaac gcgacctgga ccggtcgaga tcgccgatca
    21601 gcttcttcag cgactggcgc atggcatcca tctgctgagc cagcccgccg atttcattta
    21661 accggctgac cggcacctct ttttcgaggt gtccgtgagc aatctcctcg gcagcggcgg
    21721 aaagctcctg aaccggaaca aagatcagcc ggtgggtcag caggaaagtg gaaatggaaa
    21781 gactgatgac caccagcatg gtaatcacaa taatgataag cacctgctgg cccagctctt
    21841 cccagacatt ccggttggat gccaccagcc ggatttccgc cgcttttcct tccggaagcc
    21901 ggatcagcgt cgtgccctgc atatagcggc tttctgccac ctgatcgcga tgaaccaccc
    21961 agccgaatcc gtagattact ttattgacct ggagctccgg attctttgga tcgccgatcg
    22021 tcagcccggc aaaaacaatg tcacggtcgt tgagcagcga ttgcagaaaa tggccgatgg
    22081 ccgcctcatc acgctcggca accgggctct ccagcgcgat ttttgtgagc gaaagcttct
    22141 gctccatatt gcgctgaatt tcgctttcgg ccgcggtctt tccctgcgca atcaatacgc
    22201 ccgcaattgt aaaaagaaca atactcacca caacagtcag cacggccggc aatacaaatg
    22261 ccaggctgcg gaaaaaagat gtcgtcggca atttcatgtt tcttcggcaa aggatagcca
    22321 taccggggcc aaaaaacaac cggccagccc ctttttctat tgtccgcccc gcccgccgtg
    22381 ctacattgtg cgcctttcaa aacggagaac cctatggcag caaagaaaag cgcatacgca
    22441 caggccggcg tcgacatcga cgtcatgatg aactctcttc agcggattaa aaaagacgtc
    22501 aaatccacca gcaccaaagg cgtcatgagc gaaatcggct ccttcggcgg actcttccag
    22561 tcgcccggca aggaatttct gctggttgcc agcgccgacg gcgtcggcac caaactcaaa
    22621 gtcgcccaca tggccggcaa gcacaccacc gtcggacagg acctcgtcaa ccactgcgtc
    22681 aacgacatcc tcgtgcaggg cgccaccccg ctcttctttc tcgactacct cggcaccgcc
    22741 gcccttaaag gcaaagtatt cgaagacgtc atttccggct tctgcaaagc ctgccgcgaa
    22801 aacggctgcg ccctgctcgg cggcgaaacc gccgaaatgc ccggcctcta cccgccgggc
    22861 gaatacgacc tcgtcggcac catcgtcggc caggttgaaa agaaaaaact catcaccggc
    22921 aaaaccatcc gcaaaggcga cgtcatcatc ggcctgccct ccaccgggct gcagaccaac
    22981 ggctattctc tggcccgcaa agtcatcttc gaaaaagccg gcaaaaaact gagcgacctc
    23041 gttcccggca ccaaactgac ctttgaaaag gtgctgctcg ccattcataa gagctacctc
    23101 aaaccggtcg tcgcgctgat gaacaaagtt caggtgcgcg gcatggccca catcaccggc
    23161 ggtggactgg tcgacaacgt cccgcgcgtc ctgcccaaaa ccgttgacgc cgtcttcgac
    23221 cgctcaacct ggaaaacacc cgccatttac gaattcatcg aaaaagaagg tgacgttgac
    23281 catgaagaaa tgtaccgcgt tttcaacatg ggcatcggct ttgttctctt cgtccgtccg
    23341 gacgacgcga acaccgcgct ggcggccatc cgcaaagccg gcgaaaaagc catcatcatc
    23401 ggccgcgttg aaaaaggcac cggcatttcg cgcctgatca actaaccttc ccgcaccgcg
    23461 gttcggacgg aagggccctg tactccagta attttcaaat agtttaggtt cgagttactg
    23521 gatacggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctt cgatccactg
    23641 cggatcgcga ggcggaccgg acgagtctat tcgtgaattt atcgacaggc cccgttcctg
    23701 tttggagcga ttgaaattca gttaatgagg ggaaaataat gaaaaaaatg atattcgcag
    23761 ctttatcgtt agtaacgatg ctagcacatg gtcaaaatga tgagtggaac atcacaacga
    23821 aaaaaacaga aaagtatacc ggaattgtac tctccaacgg ccagatcgga attttaccgt
    23881 ctgaacatgt ttttggcatc aaacacataa ttctcaacaa cacgtacgat ctcgatccga
    23941 aaacggaagt gagcagaatt gtccaaggaa tgaactttgg aaatctcgaa atggagattg
    24001 atggggagaa agttaccgac agcaacattg agaattggag ccaaacgctt gatttaaagg
    24061 aagcgaaatt aaccacatca ttcacattta aagacaaagc tgctgtttca tatacgatat
    24121 atgctctgag aaatttgccg aatgctgggt atattgatgt caaggtcggg gctaaaaaca
    24181 aaattgcagt ccatgtgacc ggaaaaatct caacgccaac tgcggattat caaaagccaa
    24241 aaaacaccta caaggtatta aaagacggaa aaacgtcgat gcctatactt caaacagtgg
    24301 cattaagttc gcgcaaaaat cacacaataa gcacttctgc tacctttgtt tggcatgcca
    24361 tcaactcgtc gagagaaagt gagcgtccga aactgctcca ttccatacct tccgaacagg
    24421 aaaactgcct ctcatttgaa aaagaaatag acaaagacac ttcgcttgaa tttgcctgga
    24481 ctgcagcaga atgcacggac aagaattttg ccgacccgaa gtctgaaagc gaaaggatta
    24541 tcattaataa tctgctgact tccagagaca tactaattca acagcataaa aatctttgga
    24601 gtaaattgtg ggaaggtgac attgaaatac aaggcgatct gcaggctcaa caggatgtgc
    24661 gattggcctt gtatcatcta tattcgtttg gccggggaga taatgactta agtatcgctc
    24721 cgatgggcct ttcagtccaa accccctaca atggacatat tttctgggat tcagaattat
    24781 ggatgttccc gccgctttta atgttcaatc aagatattgc caaatcgctt gtaaactaca
    24841 gatccgatcg gctcgatgca gccaaacgaa aagcgaaaaa ttatggctat aaaggcgcca
    24901 tgtacccgtg ggaaagcgat gataccggcg aagaagcgac gcctcccttt gcattaacag
    24961 gaacttttga gcatcacata acggccgaca ttgccattgc attttggaat tattacaggg
    25021 ttacacaaga taaactctgg ctgaaagaga aaggattccc agtccttaag gaaatagcag
    25081 atttttgggt cagtcgtgcc gttaaaaatg acgacggaag ctattcgata aaaaatgtta
    25141 ttggagcaaa tgaatttgca gcaaacattg atgacaatgc ctttaccaac ggctcggcaa
    25201 tcacggcatt aaactacgcc atccaggcag ccgccgaatt agcagttgca gcagatcctg
    25261 aatggaaaaa tgtggctgat aaaataataa ttcgccgttt taaggatgga accactttag
    25321 aacactctaa atatgcagga gcgcggatta agcaggcaga tgtgaattta ttatcttatc
    25381 cactcagcat tgtgaatgac cgcgaaacaa tgcttaaaga tttgaaatat tatgaaccta
    25441 aactggacaa gaatgggccg gcaatgggca aatccatctt tgctgtcatc tatgccaggt
    25501 taggagatgc ggacaacgcc tatcgtttgt ttaatgaaag ctatatcccg aataagcatc
    25561 cgccgtttgg tgcgctttca gaaactgcta cggctaataa ctcctatttt gcgaccggtg
    25621 ccggaggatt actgcaaacc gttctgtttg gttttggagg attgcacata accgataacg
    25681 gcatagttca agaaaagcca tgcttaccaa aagcatggga aaaactcaca atcaaaggca
    25741 ttggacccga aaaaaagaca tttgagataa cccg
//
LOCUS       DAOPZO010000013        25417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_1936_length_25417_cov_7.795205, whole genome shotgun sequence.
ACCESSION   DAOPZO010000013 DAOPZO010000000
VERSION     DAOPZO010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 25417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25417
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1936_length_25417_cov_7.795205"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..1337
                     /locus_tag="PKI68_01945"
     CDS             303..1337
                     /locus_tag="PKI68_01945"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AccI family restriction endonuclease"
                     /protein_id="HNX52486.1"
                     /translation="MATTPFGIDPDIQMTGRPPTMASSEFLTNKEQGDWAEQIVFKAI
                     NDNSPDYRAVKYGRSDSLAAGDPGFEDFYQSYQDELNTIGKRPDLLIFKRRVVGDEEL
                     DLSNDGTISRAIAAIEVRSSSFLANRYSQFMETRASQAVQRCRELKSAVLQNRMRDLL
                     QQKSPEILSMIQHSNDETFRELDFRLRSWSSSAELRELSALLRELKEQIKILHKRDYL
                     SITPKIEDLALVNRWIQHFNVRHFYLQVFFDKAYIIPFRDILAIASDPSKEDVVFSVE
                     QDIKNQGKTTIKINVQVGKEILGRIDMPEHRSAMKELDRGRLLFYVTFQNGRGYLDNN
                     VFTAEIINES"
     gene            1327..2922
                     /locus_tag="PKI68_01950"
     CDS             1327..2922
                     /locus_tag="PKI68_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNX52487.1"
                     /translation="MSHDPIAQYTVSVGLEHRREHGQFFTPFDVALFMCRWAMSHGAK
                     EIYDPSFGLGAFFRAAQSVDPQVSFRASEIDQRILEFYSQTNRNPKTLHVACEDYLSV
                     WDQRHAAIVCNPPYMRFQLFNNRDAVFASFAQHLHCRLSGYTNIASAFLIKSLSELRP
                     SGRLAYIMPLEFLNTGYGKAVKSRLLTNGLLKALIRINPEKDVFPDATTSVGIVLVAN
                     DGIHSPVKFYTAPSLSCLPSLLETTPTKELAIRDLKPDDKWLKHFDGRHSNFQSCDLV
                     TIDTYGSFNRGIATGANEFFAMSNSEAERRHLPRSAFLRCITKSAQVTKSVFTEKDLD
                     ALDSGDSPVLLLNLNGVVNGAPSDYLKYGEEQGYHERYLTKVRTPWYKLEKRTPAPLL
                     FGVFSREKFKVIRNLSTAVNLTCYHGFYPNLFGQDILDSLFLYFQSRAARRLLELNMR
                     RYGDSLDKFEPNDLNHALAPSIDWFAKLSPEAVDSALESCRQGEGIPDFLNEIFDELI
                     SDAEQSHGTLWRAARGGLAEVPA"
     gene            complement(2929..3687)
                     /gene="hisF"
                     /locus_tag="PKI68_01955"
     CDS             complement(2929..3687)
                     /gene="hisF"
                     /locus_tag="PKI68_01955"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNX52488.1"
                     /translation="MLAKRIIPCLDVTGGRVVKGVKFVELRDAGDPVECAKAYEAQGA
                     DELTFLDITASSDGRDTMVDIVRRVAEQVFMPLTVGGGIRTPADVRRMLNAGADKVSF
                     NTAAIKTPEILNQCSEQFGSQCTVLAIDARRNEKGGWTVYTHGGRNPTELDAVEWAQE
                     GVARGAGEILLTSMDADGTKNGYDLELTRAISEAVGVPVIASGGAGTLDHLVEAVTIG
                     KADAVLAASIFHFGEFTIRQVKEYMRSKGVVVRL"
     gene            3845..4198
                     /locus_tag="PKI68_01960"
     CDS             3845..4198
                     /locus_tag="PKI68_01960"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNX52489.1"
                     /translation="MRKVRAKGFTLVEVMLVISIIGFLAALGIPAILNAYAKSLETSR
                     ARNIAEIEKAKGVLTLPKGIGMVGAMSLGEKDTFDTPAVISNLCAVMRIKDISELTVG
                     DVPIQVGDLKNKAYY"
     gene            complement(4214..5809)
                     /locus_tag="PKI68_01965"
     CDS             complement(4214..5809)
                     /locus_tag="PKI68_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNX52490.1"
                     /translation="MDISEQTSNEEQSGAFFSREAVSGVPWMVASKAVLFFVYFAISV
                     LTVRLMGSTNYGIYSICINIAEWAGVFCGLGLVSALFRFVPELVVQHNAAGLKRLVWK
                     SLWLQLAATAAGTLILILMKPWLNNWFHIDFGSYLVLTGLLIGFSLLKENVSAVETSL
                     FRTKHISILTLARGILWLGLVVTMLRIVPTVASAFWTQILSYAGVYLIGAWLMFRHIY
                     SFDWRSPPYGIGKRRTAKYSFAILSNTIVRMMMMKYTEVFFLGLTVTPAVIGAYDLGY
                     SLPPLIIFFIPDSLHMLFTSGFAEAYSRDPNCLGRLIKAYYKMTILFMVPLAIFGFFF
                     APEAVVMVYGDNMALAGVLASGFCLVHLFSTISTPLSMAIKAKEQIMATMPVMILQIV
                     INLFLDWLLIVYFDLGVWGGMGAVAGTFLISAPIRLSVVKKILGGIFFPTEYMLRMTF
                     TLFILSSILTVASDHWNLLGLFETRWLNVVTLFGLGVIYLLLFFVAIRMFRLVRDEDV
                     ADFRALDIKKLNFVFDLLSPRKS"
     gene            complement(5816..6763)
                     /locus_tag="PKI68_01970"
     CDS             complement(5816..6763)
                     /locus_tag="PKI68_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52491.1"
                     /translation="MSSKTIVTACDRNFVWGAFLLLLSLRRNGVTLPVHVVTHNLGEV
                     DRALLHQIDGVTLFAASDEANHRNIVYQKTAAILTAETDLILWIDSDCIVTGDITPLI
                     EASGEGLQIRMRPEAEIAYLFKKRYAAGEKRGTLPRRVLDIWRSDVGERPVPAINTSC
                     NAHCFTVHRKYLDFIRRWDRQIRKVIPDDTSGGVVNDRSFAYFLLDEAVMSSMLAFAE
                     GPPPIRRYLMEDDPACAVVHFGGRPKPWTRWLLRNMEYFDEVTGLLEWAETQGWVLPY
                     KPWYLQRRHKILCRIHALIFEAQRHIRRNVHGLKKKNYS"
     gene            6829..7689
                     /locus_tag="PKI68_01975"
     CDS             6829..7689
                     /locus_tag="PKI68_01975"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNX52492.1"
                     /translation="MKKAGVIANPKRPHAADIFERLTRKARELGIELFADKQTADYLP
                     AATVLEFNEFAGTVDVLLALGGDGTVLFCANLLKGADVPILGINLGSLGFLTGTGEDS
                     LEKTLDALAGGKCRRDVRTVADCTVLHNGKPAAGYRILNDTVIGFGGSSRIITLGLSV
                     NGETLSSFACDGMVVTTPTGSTGHFLSSGGPIIQPGADVFGISVICPHTLSNRPLILP
                     DTSAIEITVQHTHKKLILSADGQDVDELDAGDAVRIVRSDRPVTFLHLPGYSYFSVLS
                     QKLHWRGSNY"
     gene            7689..8516
                     /gene="lgt"
                     /locus_tag="PKI68_01980"
     CDS             7689..8516
                     /gene="lgt"
                     /locus_tag="PKI68_01980"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNX52493.1"
                     /translation="MNLFLPILGTYVHHLNPVIFQIWGPLAVRWYGLAYLLGFLGGYL
                     LLLRMSKRGEFAVPPQELGNFIVHIAIYGVFLGGRLGYVLLYGWETFVRDPLFFFRVW
                     EGGMASHGGMIGVFLVVLWTAWKKKCAFWNITDGLALVAPVGLFFGRIANFINGELWG
                     RATNVPWAVIFPQAEDTTPTPRHPSQLYEAFGEGLLLFTVLQLLRRTAWGKREGVLSA
                     VFLILYAAARITSEFFREPDSTIYFGWLTKGQLYSSFMILGAVVILWRKKLLCKASS"
     gene            8498..9877
                     /locus_tag="PKI68_01985"
     CDS             8498..9877
                     /locus_tag="PKI68_01985"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF006688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNX52494.1"
                     /translation="MQSILIKNANVINEGTQRSADVLIRHGRIDRIDREISADADILI
                     DAGGKALLPGMIDCHVHFREPGLTHKGDMQSESRAAAAGGVTSVMEMPNTSPPAVTNG
                     RLEEKFAIAAQKMAVNHSFYLGASLHNLDQIKAVDPKNVCGVKVYMGSSTGDMLVDHL
                     QTLEELFRECPVNLTAHCEDAGIIKKHEEIFRELYGDKTPFSAHPHIRSEEACFCSTQ
                     LAVDLARRHGTRLHVLHISTARELELFDGGTVRVSSPRENECFIERDETVRKITAEVC
                     VHYLHFTSSVYETKGALAKCNPAIKRASDRDALLRGLLTDRIDTIGTDHAPHTWQEKQ
                     GTYWTAPSGMPVIQSALPAVLEYFHSGLLPLETIAAKIAHNPARIFNVKERGFIREGY
                     WADLVLVDLNRPQTVSKETILYKCGWSPFEGETFRSSVDTTIVSGQIAWQNGKINDAV
                     RGRRLEFCR"
     gene            complement(9902..10480)
                     /locus_tag="PKI68_01990"
     CDS             complement(9902..10480)
                     /locus_tag="PKI68_01990"
                     /inference="COORDINATES: protein motif:HMM:NF022215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 family protein"
                     /protein_id="HNX52495.1"
                     /translation="MNRFLRYKDRLLTGAAFAALLSVTVLQLHNQGRIWWCACARPFL
                     WDGDIRSAHTSQHLADPYSFTHILHGVIFYGLIRLVFPRLRLIWRLWCAVLVECLWEI
                     LENSSFVIQRYRETTIGLGYTGDSIANSLSDITCCALGFFIARRIGFWRSAVLFAATE
                     LVLLFWIRDNLTLNVLMLLYPVEAIKSWQMVH"
     gene            complement(10487..11059)
                     /locus_tag="PKI68_01995"
     CDS             complement(10487..11059)
                     /locus_tag="PKI68_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNX52496.1"
                     /translation="MLIRETTLTVLDFETTGSVPGFDTQPWQVAAVVLQNGRVDPQQT
                     FKSLVRVESNRPFNAYAPGKHHKLRDEIAAADEVSGVWKKLEGRVAGRPLAAHNVATE
                     KKFLRQMAPMHHFGPWVDTLALARQAWPKAPSHKLEDLISGLKLEARVRELCPHGEAH
                     DALYDAFACAVLLEHLLTLPGWQNLSVSDG"
     gene            complement(11063..11269)
                     /locus_tag="PKI68_02000"
     CDS             complement(11063..11269)
                     /locus_tag="PKI68_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52497.1"
                     /translation="MKRFVLDRREDETGVSGTGYIAEGVLFDNGKCALAWLSRYCSIT
                     IFDNLDELKAVHGHDGKTVIQWID"
     gene            complement(11358..11960)
                     /gene="rpsD"
                     /locus_tag="PKI68_02005"
     CDS             complement(11358..11960)
                     /gene="rpsD"
                     /locus_tag="PKI68_02005"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNX52498.1"
                     /translation="MKYNGPKIKKARRLGIALSPKCEKFLERRPHPPGQHGPSKRRNK
                     LTDYGKQLIEKQRLQYQYNLTERQLRGYYAKAARKVGNTADIMLQLLETRFDAVVLRA
                     GFARSIYQARQLVGHAHFLINGKKVNIPSYQMRIGDVITVREGSRELEIFPSAQQIAS
                     TPAYLTVDDKKLTASLSRLPEHGEVPVQCEASLVVEFYSR"
     gene            12209..13438
                     /locus_tag="PKI68_02010"
     CDS             12209..13438
                     /locus_tag="PKI68_02010"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: protein motif:HMM:NF005121.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HNX52499.1"
                     /translation="MKEVKKVAILTAGGLAPCLSSAIGGLIERYTAVAPKVKIICYTS
                     GYKGLLLGESITVTPAIRTKASVLHKHGGSPIGNSRVKLTNVKDCVKRGLVKEGENPL
                     EVAARQLVKDGVDVLHTIGGDDTNTTAADLAAYLKDNNYDLTVVGMPKTIDNDVFPIR
                     QSLGAWTAAEEGAKYFENVVAEHNANPRMLIIHEVMGRNCGWLTAYTAQVYRDRLAKM
                     EFVPGIGLSKIRKDVHAIFVPEMAIDFKAEAARLKKIMDENDCVNIFISEGAGVEEIV
                     AQMQAAGEEIARDAFGHVKLDTINPGKWFGKQFAEMLKAEKVLVQKSGYFARAAAANK
                     EDLKLIKRCANKAVECALKRVSGVIGEDEDQKDVLRAIEFPRIKGGKPFDIDVKWFGK
                     LLAGIGQAKGPKVAVKH"
     gene            13491..13856
                     /locus_tag="PKI68_02015"
     CDS             13491..13856
                     /locus_tag="PKI68_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52500.1"
                     /translation="MKTAPFKICPMCSKHWETLETFLSDPELALAGYQINFANLKGGL
                     FYFSHIKTGCGTTMVIPVGEFTGLSNRPMLASRGKQPDCCPELCVRKNELDPCPVECE
                     CVWVREIMQVIRNWEKRSA"
     gene            complement(13853..15265)
                     /locus_tag="PKI68_02020"
     CDS             complement(13853..15265)
                     /locus_tag="PKI68_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNX52501.1"
                     /translation="MNSIHPSSSSSYRALLRVAVPLIITSTSFMVQNFCARMFLAWYS
                     PETLQAAVPAGILFFTLVCGFMATAAFANSLVAQYYGNEDYHGCSRSVAQAILLALFS
                     FPLILLLIPAGLWMLSVSGHSPAVLIEEKTYFFILMLGGVQFPLSSAVSSFFSGRGLT
                     KVVMLCAIAGAALNLLLNWLLIFGVGPFPEMGMAGAALASVISGFVSPVIMLALYFSP
                     KNNRLYDTRRLFYFDAPLFRRLLRFGVPAGIHMALDIGAFSLFVLLIGRLGETAFMAA
                     NIALSVNMIAFMPSIGIGQAAGTLVGQHIGRGDLDGASAVGWRAVHIGWIYTVCIALT
                     FVLTPEIYIHLFARGDIPFAEISGLARTLLLFAACWGLMDATNLIISGALRGAGDTHF
                     VMWYETSMAWFFFAAGELLIVLVFKWGVLAAWGWALIYICLLAFGMVARFRSERWQLI
                     ELIERQETLPETSADGMPGI"
     gene            complement(15347..16471)
                     /locus_tag="PKI68_02025"
     CDS             complement(15347..16471)
                     /locus_tag="PKI68_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52502.1"
                     /translation="MHTKGAAVGTIGIITGSAILGAAAAVSVYHAMQGGVETISTTGL
                     MSFLLTLGLSVSAIVLAIAAIALCRMAELSIAKKSEETSAMQAAMVEKTVSAIERMES
                     SVARIGEEIADTLYENFEMLAGEIQEKLPSRDVLRADVNEAVERALSLRAGLKISEKE
                     LPGNPVVTENPPAAKPVSAAEPAAVISAPEPEPQVAAVADKMREKADKKYGEFKDIVL
                     LGVANYPGVLARKIGEGQYRTGGDDLVDGAFVVNNEKVAVCTFCTNDIITDRFMGDTG
                     DGFNVFLRSLVNELKSGHFTRVFLVFDGKLANTSPYAKSLNALSSRIDAETFACFELF
                     EGSPDIIIPELTERVSRLMELPAAETNEVPELTFRRQMGA"
     gene            complement(16629..17291)
                     /locus_tag="PKI68_02030"
     CDS             complement(16629..17291)
                     /locus_tag="PKI68_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNX52503.1"
                     /translation="MNHEIMTIEEVASYLRVSERTVYDWAQKGQLPGGKLGTAWRFKR
                     SDIEGWVNRCIGSSAAPAPVAPGLETLSRILSPERVVFFDTNEKTAALKTLCAALGTS
                     PLIHNEAELEQAIFKREELMSTGIGFGVGVPHVRLASVDDLVMALGIARQPLDDYSSL
                     DEIPVQIVCMVAASGTQHPQYIRALSAISSRLKDQSMRKALIEAKNAVSAFDIFTGGV
                     PS"
     gene            complement(17349..18110)
                     /locus_tag="PKI68_02035"
     CDS             complement(17349..18110)
                     /locus_tag="PKI68_02035"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNX52504.1"
                     /translation="MKLFDVHCHLQDQALFPDIKNVMERAAAAGVERFVCCGTSSGDW
                     QTVVQLAEQFPQVIPMVGIHPWFVSTGWLKHVQMLEKLLEEHPEAGVGETGLDFQEKC
                     TNRVEQEECFAAHLDMARELNRPVAVHCVRAWGRLVEILRVHPAPRVLLHAFGGAAEL
                     IPELVELNCWFSFCGSAANPQSKRVRASAAAVPNGRLLIETDSPDFPLAGCLPPNEPA
                     NLAVVVRAIAELRGIPVEKLAALTFTNSEQFFASV"
     gene            18283..20058
                     /locus_tag="PKI68_02040"
     CDS             18283..20058
                     /locus_tag="PKI68_02040"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF006577.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HNX52505.1"
                     /translation="MNIKIETSRDSLLSEFGLKTLESRYMLPTEKSPQEAFARAAAAF
                     SDTPEMAQRIYDYASMLWFMFATPILTNGGTNRGLPISCFLNYVPDSRGGITGHYTET
                     AWLSSFGGGVGGYWGDVRACGTRTSHGSESTGVIPFLKVVDAEMLAFSQGVTRRGSYA
                     AYLPVDHPEIEEFLDVRKPTGGDVNRKSTNLHHGVVLTEEFMNLIDQATRIPGFDDSF
                     NLVDPHTKEVKKTVSAKTIWVKLIQNRIETGEPYILFDKAVNDALPEFQKKLGLRVHQ
                     SNLCSEITLPTNAERTAVCCLSSVNLETYDEWKDHPHFISDLVRFLDNVIQSFIDNAP
                     DEIGRARFSAQRERSIGLGAMGFHAYLQAHSIPFESAIAKGLNLNMFKHIKEQASAAT
                     QKLAEERGECPDAAGYGVRNAHLLSIAPNASSSIICGNTSPSIEPFRANAFTQKTKSG
                     SALMKNKYLEEALERHGQNNSEVWKSIITQGGSAQHLDFLTDWERDVFKTALEIDQRW
                     IIEFAADRQQYVCQSQSVNLFFPANVSKQELHNTHMLAWKKGLKSLYYVRSEAIKRAD
                     VVSEQKPREIMLDYNKETTCLSCEG"
     gene            20149..21108
                     /locus_tag="PKI68_02045"
     CDS             20149..21108
                     /locus_tag="PKI68_02045"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF007186.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HNX52506.1"
                     /translation="MPLLEERTYYKPFEYDWAFEAYKMQQQMHWIPDEVNLADDLKDF
                     NENIPQKDKNLLLNIFKFFTQADVDVAGGYGTLFLPTFKIPEVRMMLSAFAAMEAVHQ
                     DAYSLLLETLGLSDDEYTAFMSIAEMKEKHEYLLNFNMNTPFEIAKTLAVYSAFTEGI
                     QLFSSFAILMNFPRFNKMKGMGQIVTWSIRDESLHVESMALLFKAFIKEHPELWTDKL
                     KYEIYCAAERSVELEDNFIDIAFELGAVEGLTPDEVKQYIRYIANRRLIGIGMKSIYT
                     VPENPLPWLDYMLNGVEHTNFFENRSTEYAKASTTGNWQDIFK"
     gene            complement(21210..21596)
                     /locus_tag="PKI68_02050"
     CDS             complement(21210..21596)
                     /locus_tag="PKI68_02050"
                     /inference="COORDINATES: protein motif:HMM:NF021429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family DNA-binding protein"
                     /protein_id="HNX52507.1"
                     /translation="MSVEEKKNNPLHGLSAEAMVTALVEFYGWEILYAALGLNCFRMN
                     PSISSAVKFLRKTDWARHKVENFYLYRFKQMPKARGEEFDLDPRDRGFANGVVPKDPM
                     PLTVELIAEMKAEAEENYQNRRGGRR"
     gene            complement(21739..22536)
                     /gene="thiF"
                     /locus_tag="PKI68_02055"
     CDS             complement(21739..22536)
                     /gene="thiF"
                     /locus_tag="PKI68_02055"
                     /inference="COORDINATES: protein motif:HMM:NF006395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HNX52508.1"
                     /translation="MNIRLNERPVETGAKTLFALRDAVKPSADVVILNGAAMTADVLL
                     KAGDEVSLIRRGETPSADELEALMASRHTPGVHARLKAATVGIAGLGGLGSAIAVALA
                     RTGVGKLIVADFDVVEPSNLNRQQYFVDQIGMLKTDALVSNLRKINPYVQVEAHAVKL
                     TPDNLFPTFGAVDVMIEAFDRADQKAMLLQAFTAQKPGVPFIGASGMAGFGPEETIGV
                     KKMGRNVYIVGDLETAAQPGCGLMAPRVGIVASMQANLALRLIIEND"
     gene            complement(22623..23060)
                     /locus_tag="PKI68_02060"
     CDS             complement(22623..23060)
                     /locus_tag="PKI68_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNX52509.1"
                     /translation="MENWYLYIIAGLAAGIMSSLFGVGGGVLIVPILALGFSFGQKSA
                     QGMSLFIMLPIALAGAIRYKLNPDVPINLPVCGLMALGGIAGAMLGTHLVFGISDIVL
                     KRIFAVFIILTGIHMIVKSFSAPAGKELSQQQIEDKFTEGNNG"
     gene            complement(23061..23999)
                     /locus_tag="PKI68_02065"
     CDS             complement(23061..23999)
                     /locus_tag="PKI68_02065"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNX52510.1"
                     /translation="MSQTDQLATVNVPQTAQPDMDTFWLAARRRCEEVPLNVKLSTDS
                     SPDIRDLTFDGLDGTPIHAWLLLPPEAKDHKVPVVIYCHGANRSRGAPDDFSHWLAAG
                     CAVISPDFRLQCGTTGSVTAFDGDGKSGWWTLNLNDLMNSYLYCVWTDFLRAVRLARE
                     TPEIDPERIAAEGHSQGGGMALGLAALDCSVSLCMADVPSSCRMEHRIATRSGGGAGI
                     AKYLAMFPERIETVLRNLSYFDNINLAPDITCPVLVSCGLKDTVCPPECTYAAYNKIR
                     AVKEMAVYAHAGHEGGRLEHRVRKLEFLKRFFERKE"
     gene            24048..>25417
                     /locus_tag="PKI68_02070"
     CDS             24048..>25417
                     /locus_tag="PKI68_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNX52511.1"
                     /translation="MKAYRPFIVILLAALILPGAGNSQSISADTGKKPLVYQIPIQDQ
                     IEPALLYVVRRGVAEAAREKAAAIVFVMNTPGGGVKEAREIISLIGQIDVPVYTFVEK
                     DAYSAGAIIALSTKHIYMAPGSVIGAATPLVMSPFGGVEKMPDAIEEKMTSAVAALVR
                     AAAEQGGHDPQLGEAMVRRELGYTAGTNVLSKAGELLTLTDSEARGILSEGTVADVPA
                     MLKAAGLPDAEIKTLEVTPAEKIARFIAAFAPILLMIGLGGIYIEIKTPGFGLPGILG
                     TTALVLFFFGHHIAGLAGMEDIILFIVGFSLLLVEIFILPGHIVFGVSGIGLILISLL
                     NAMTWKIPGHFWPSLSDSTWHYAIGNLALGLTGTVALGIFAGKYLPKSRVLRPMVLSQ
                     QTNRAAGFTAAHDRSDLLGKEGIAEMNLRPAGRALFGNDRVNVIARGEFIEKGSAVRV
                     IETSGSR"
BASE COUNT         6138 a         7338 c         6715 g         5226 t
ORIGIN      
        1 tagggcgggg tccgtgaccc cgccggagac cgaggccacg ggcctcggct acaattttgg
       61 ctcatcgttt tctccgttaa aagtcggcgc aatatacaca gatgccacga atcctgccag
      121 tctcttcccg ccacattcaa aaagtattta ggattgtcgc tactgagccg ccgcgctaca
      181 ttcgaggcgt tggcaaatcg atccgttaaa aaccaaagtc aggcaaataa cactgttcgt
      241 caaagattag aagataggga aaatagatgc cacagtcata caaacagacc atagcgcgcc
      301 tcatggccac aacccccttt gggatcgatc cagatatcca gatgacgggc agaccaccga
      361 cgatggccag ttctgaattc ctgaccaaca aagaacaggg agattgggcg gagcagattg
      421 tcttcaaggc aatcaacgat aattcccccg attaccgcgc cgtgaaatac ggacgttctg
      481 actctctggc cgcaggagat cctggctttg aagacttcta ccaatcctat caggatgagt
      541 tgaacaccat cggcaagcgc cccgatctgt tgatcttcaa acgaagagtt gttggggatg
      601 aggaactcga tctttccaac gacggaacaa tttcaagagc aatcgcagcc atcgaggtgc
      661 gctcaagttc tttcttggca aaccgatata gtcagttcat ggaaacacga gccagccaag
      721 cggttcagcg gtgtcgcgaa ttgaaaagcg cagtgctgca aaatcgaatg cgagacctgt
      781 tgcaacaaaa gagccccgaa atactttcta tgattcaaca ctcgaacgac gaaacgttca
      841 gagagttgga cttcagactg cgttcatggt cgtcgtctgc cgaactacgg gaactttcgg
      901 cattattgcg tgaactaaaa gagcagatca agatactgca caaacgggac tatctcagca
      961 ttaccccaaa aatcgaagac cttgctctcg tcaacagatg gattcagcac ttcaacgtgc
     1021 gccatttcta cctgcaagtg ttctttgaca aggcatatat tatccccttt cgtgatatcc
     1081 ttgcgattgc ttctgaccca tcaaaggaag atgtggtatt ttcggtagag caggacataa
     1141 agaaccaagg gaaaaccacc ataaaaatca atgtccaagt cggaaaagag atactcggac
     1201 gcattgacat gccagaacac agatcggcga tgaaggagtt ggatagaggc agactcttgt
     1261 tctatgtcac cttccagaat ggccgtggat acttagacaa caacgttttc acagcggaaa
     1321 tcattaatga gtcatgatcc tatagcacag tacacagtct ccgtgggtct tgaacaccgg
     1381 cgtgagcacg gacaattctt tacgccgttc gatgttgcct tgttcatgtg tcgctgggca
     1441 atgtcgcatg gagccaaaga gatctatgac ccatctttcg ggctaggcgc attctttaga
     1501 gccgcacaat ctgttgatcc tcaagtatca tttcgagcat cggaaatcga tcagcgcatc
     1561 ctcgagttct attcgcagac caataggaac cccaagacac tgcatgttgc ctgcgaagac
     1621 tatctttcgg tatgggatca gcgtcatgcg gccattgtat gcaatccacc ctacatgcga
     1681 tttcagttgt tcaataatcg cgatgccgtc tttgcgtcct ttgcgcaaca cttgcactgt
     1741 cggttatcag ggtacacgaa tatcgcctcg gcatttttaa ttaaatcgct ctcagaactt
     1801 cgcccttcgg ggcgccttgc ctacatcatg cctctcgaat tcttaaatac gggttatgga
     1861 aaggcggtaa agtcgaggtt actcacaaat ggcttgctaa aagcactcat ccgaattaat
     1921 cccgagaagg atgtgttccc cgacgcgaca acctccgtag gcattgttct tgtggcaaac
     1981 gacggaatac attcaccagt caagttttat acggcaccga gtctatcctg tctgccctct
     2041 ttgcttgaaa caacaccaac gaaggagttg gccattcgcg acttgaaacc cgacgacaaa
     2101 tggctaaagc actttgatgg gagacactcc aactttcagt cctgtgatct agtcaccatt
     2161 gacacatacg gttcattcaa ccgaggcata gccacagggg caaatgagtt ttttgccatg
     2221 tcaaacagcg aagcagagcg ccgtcatctc ccgcgttctg cctttcttcg ctgcataacc
     2281 aagagcgccc aagtaacaaa gagcgttttc actgaaaagg acttggatgc tctagattca
     2341 ggcgactccc ctgtattgct actcaatttg aacggtgtgg taaatggcgc tccaagtgac
     2401 tacctgaaat acggagaaga acagggttat cacgaacgat atctaacaaa ggtcagaaca
     2461 ccgtggtaca aactggagaa gcgaaccccg gcacctttac tgtttggcgt cttttcgcga
     2521 gagaagttca aagttattag aaacctaagc accgcagtta atcttacatg ctaccacggc
     2581 ttctatccga acctgtttgg gcaggacatc cttgactctt tgtttctgta tttccaatca
     2641 cgtgcagcgc ggcgactcct tgagcttaat atgcgtagat atggagactc gctcgataaa
     2701 tttgagccta atgatttaaa tcacgcatta gcgccgtcaa ttgactggtt cgctaagtta
     2761 tcacccgaag cagtcgacag cgcgcttgaa tcgtgccgac agggtgaagg gattcctgat
     2821 tttctgaacg agatatttga tgaactcata tcagatgccg aacaatcgca tggaacacta
     2881 tggcgagcag cccgtggcgg gctcgccgag gttcctgcgt gacgacaatt acaaccttac
     2941 aactacgcct ttggagcgca tgtattcttt gacctgacgg atggtgaatt cgccgaagtg
     3001 gaaaatcgag gcggcgagga cggcgtcggc tttgccgatg gtgacggctt cgaccagatg
     3061 atccaacgtt ccggcgccgc cggaggcgat gaccggtacg ccgaccgctt cggaaatggc
     3121 gcgggtgagt tccagatcgt agccgttctt ggtgccgtcg gcatccatgc tggtgagcag
     3181 gatttccccg gccccgcgcg cgacgccttc ctgcgcccat tcaaccgcgt cgagttcggt
     3241 cgggttgcgt ccgccgtgcg tataaaccgt ccatccaccc ttttcgttgc ggcgggcgtc
     3301 gatggccagc acggtgcact gggagccgaa ctgttcggag cactgattga gaatttccgg
     3361 ggttttgatc gcggcggtgt tgaaggaaac tttgtcggcc ccggcgttga gcatccggcg
     3421 gacgtcggcg ggcgtgcgga tgccgccgcc gacggtgagc ggcatgaaaa cctgttcggc
     3481 gacgcggcgg acgatgtcga ccatggtgtc gcggccgtcg ctggaggcgg tgatgtcgag
     3541 gaaggtcagc tcgtccgcgc cctgcgcttc gtaggctttg gcgcactcca ccggatcgcc
     3601 ggcgtcgcgc agttcgacga atttgacgcc tttgacgacg cggccgccgg ttacatccag
     3661 acaaggtatg attcgttttg ccaacatagg ctgtgaagat aaccacgatt gccccgtccg
     3721 gcaaagcgga aatgcaccgg tttgcgcaca gattcaaacc gggcggcgtt tattaaaaat
     3781 tggcttttcc ggcgcagggt ctgctacaat gttcacggct tcgaaaacgg aagaaagacg
     3841 gttcatgcga aaagttcggg caaaaggttt tacgctggtt gaggtcatgt tagtgatctc
     3901 aattatcgga tttctggccg cgctcggcat tccggctatt ctgaatgcct atgccaagtc
     3961 gctggaaact tcccgggcgc gcaatatcgc ggagattgaa aaagcgaaag gtgttctcac
     4021 gctaccgaaa ggaataggta tggtaggcgc catgtcactt ggagaaaagg acacgttcga
     4081 caccccagca gtaatcagca atttgtgcgc ggttatgcgg atcaaagata tcagcgaact
     4141 gaccgtcgga gacgttccga tccaggtcgg cgacctcaaa aacaaagcct actattgatc
     4201 gcccggcgga aaatcaactc ttccgcggtg aaagcagatc aaaaacaaag ttgagctttt
     4261 tgatgtcgag agcgcggaag tcggcgacgt cttcgtcgcg caccagccgg aacatccgga
     4321 tggcaacgaa gaaaagcagc agatagatca ccccgagtcc aaaaagggtg accacattca
     4381 gccagcgggt ttcaaaaagc cccagcaggt tccaatggtc ggaagccacg gtcaggatgc
     4441 tcgacagaat aaagagtgtg aatgtcatgc gaagcatata ttccgtcggg aaaaagatgc
     4501 cgcccagaat ttttttgacc acggaaaggc ggatcggcgc ggaaatcagg aatgttccgg
     4561 ccactgcgcc catgcctccc cagacgccga gatcaaagta aacaatcagc agccagtcga
     4621 ggaacagatt gattacaatc tgaaggatca taaccggcat ggtcgccatg atctgttcct
     4681 tggccttaat tgccatggat agaggcgtag agatggtgga gaacaggtgg acaaggcaga
     4741 agccggaagc aagtactccc gcaagagcca tgttgtcgcc gtaaaccatg accaccgcct
     4801 ccggagcgaa gaaaaagccg aagatggcca gcggaaccat gaagagaatg gtcattttat
     4861 agtacgcttt gatgaggcgg ccgaggcagt tcgggtcgcg actgtaggct tcggcaaacc
     4921 cggaagtgaa aagcatgtga agtgaatcgg gaataaagaa aataatgagc ggcggcaggg
     4981 aataaccgag gtcgtaggcg ccgatcacgg cgggcgtgac ggtgagaccg aggaagaaga
     5041 cctcggtgta tttcatcatc atcatgcgta cgatcgtatt gctgagaatg gcaaaggaat
     5101 atttggctgt gcggcgtttg ccgattccgt acggcggaga acgccagtcg aaactgtaaa
     5161 tgtggcggaa catgagccat gcgccgataa ggtacacccc cgcgtacgaa aggatctgtg
     5221 tccagaaggc cgaggcgacc gtcggtacaa tccgcagcat ggtgacaacc agtccgagcc
     5281 agagtattcc gcgtgcaagc gtcaggattg agatgtgttt ggtccggaac agcgaagttt
     5341 caactgcgga aacgttttct ttcagcagac tgaacccgat cagcagcccg gtcagcacca
     5401 aatacgaacc gaagtcgatg tggaaccagt tgttcagcca cggcttcatg agaataagaa
     5461 tcaacgtgcc ggcggctgtc gcggcaagct gcagccagag cgatttccag accagcctct
     5521 tgaggccagc cgcattgtgc tgcacgacca gttccggcac aaaacggaac agcgcggaaa
     5581 caagtccgag gccgcagaaa acgccggccc attcggcaat gttgatgcag atggagtaga
     5641 ttccgtaatt ggtgctgccc atcagccgga ccgtcagcac ggaaatggca aaatacacaa
     5701 aaaagagaac cgctttgctg gcaaccatcc acggaacacc ggaaacggcc tcgcgggaaa
     5761 aaaatgcccc gctctgctct tcatttgaag tctgctcgct tatatccata cgttctcaac
     5821 tgtagttttt tttcttaagc ccgtgcacat tgcgccggat atggcgctgc gcctcaaaaa
     5881 tcaaagcatg gatccggcag agtattttgt gcctcctctg aaggtaccat ggtttatacg
     5941 gaagcaccca gccctgcgtc tccgcccatt caagcagccc ggtcacttcg tcgaaatatt
     6001 ccatattgcg caacagccag cgcgtccacg gcttaggccg tccgccgaag tgaaccacgg
     6061 cacaggccgg gtcgtcctcc atcagatagc gccggatggg cggcggtcct tcggcaaaag
     6121 ccagcatgga gctcataacc gcttcatcga gcaggaagta agcaaaactg cggtcgttca
     6181 cgaccccgcc ggacgtatca tccggaatca ctttgcggat ctgccggtcc cagcggcgga
     6241 taaagtcgag atacttgcgg tgcacggtga agcagtgagc gttgcaggag gtgttgatcg
     6301 ccggcaccgg gcgttcgccg acatcgctcc gccagatatc gagcactctg cgcggaagtg
     6361 ttccgcgctt ttcgccggcg gcatagcgct ttttgaacag ataggcgatt tccgcttccg
     6421 gccgcatccg gatctgcaat ccctcgccgg aggcttcgat cagcggcgta atgtcgccgg
     6481 tcacgataca gtcggaatca atccacagga tcagatcggt ttccgccgtc aggatggcgg
     6541 cggttttctg gtaaacgatg ttgcggtggt tcgcctcgtc cgaagccgca aagagagtta
     6601 cgccgtcaat ctgatgcagc agcgcgcgat ccacttcgcc gagattatgc gttaccacat
     6661 gcaccggcag agtgactccg ttgcggcgca gggaaagaag cagcagaaaa gcgccccaga
     6721 caaaattgcg gtcacaggcg gtaacaatgg ttttagaact catgtgcagt gtccatttcc
     6781 ccctgaataa atcttgtgcg cccgaccggt tgtggcaaca atcaccgtat gaaaaaagcc
     6841 ggtgtcattg caaaccctaa acgcccgcac gccgccgaca tctttgagcg gctgacccgt
     6901 aaagcccggg agctcgggat cgaactgttt gccgataaac agacggccga ctatctgccc
     6961 gctgcaacag ttcttgaatt caatgagttt gccggaacag tcgacgttct gctcgcgctc
     7021 ggcggcgacg gcacggttct cttctgcgcc aacctgctca aaggtgccga cgtaccgatt
     7081 cttggaatca acctcggcag cctcggattc ctcaccggca ccggtgaaga ctcgctcgaa
     7141 aaaacacttg atgcactggc cggcgggaaa tgccggcgcg atgttcgcac cgtggctgac
     7201 tgcacggttc tgcataacgg caaaccggct gccggctacc ggattttaaa cgacacggtc
     7261 atcggcttcg gcggctcttc gcgtatcatc acgctcggcc tctctgtaaa cggcgaaacg
     7321 ctttcctctt tcgcctgcga cggcatggtg gtcaccacgc cgaccggaag caccggtcac
     7381 tttctgtcat ccggcggccc catcatccag cccggcgcag acgttttcgg catcagcgtc
     7441 atctgcccgc atactctaag caaccggccg ctgattctgc cggacacaag cgctatcgaa
     7501 atcaccgtac agcacacgca caagaagctg attctctccg ctgacggaca ggacgttgat
     7561 gaacttgacg ccggcgacgc cgtccggatc gtccgcagcg acagacccgt caccttcctg
     7621 cacctgcccg gctacagcta cttttccgtt ctaagtcaaa aactccactg gcgaggctcc
     7681 aactactgat gaatctgttt ctcccaatac tcggcaccta cgtccaccat ctcaacccgg
     7741 tgattttcca gatatgggga ccgctggcgg tccgctggta cggtctcgcg tacctgctcg
     7801 gattcctcgg cggctacctg cttctgctcc ggatgtcgaa acgcggcgaa ttcgccgtac
     7861 cgccgcagga actcggcaac ttcatcgtcc acatcgccat ttacggcgtc tttctcggag
     7921 gccggctcgg ctacgtactc ctttacggct gggaaacctt cgtccgcgac ccgctcttct
     7981 ttttccgcgt ctgggaaggc ggtatggcca gccacggcgg aatgatcggc gtatttctgg
     8041 tggtgctctg gacggcatgg aaaaagaaat gcgccttctg gaatatcacc gacggcctcg
     8101 cactcgtcgc gccggtcgga ctcttcttcg ggcgcatcgc caacttcatc aacggcgaac
     8161 tctggggccg ggcaacgaat gttccgtggg ccgttatctt cccgcaggcg gaagacacca
     8221 cgccaacacc ccgccatcct tcacagctct acgaagcatt cggcgaaggc cttctgctct
     8281 tcaccgtact gcagctcctc cgccggaccg cctggggcaa acgcgaaggc gtcctgagcg
     8341 cagtcttcct catcctgtat gccgccgccc gcatcacgag cgaattcttc cgcgaacccg
     8401 acagtaccat ctacttcggc tggctcacca aaggtcagct ctattcttca tttatgattc
     8461 tgggcgccgt tgttattctc tggaggaaaa aactcctatg caaagcatcc tcataaagaa
     8521 cgcgaatgta attaacgaag gaacccagcg gtctgccgat gttctgatcc gccacggacg
     8581 gattgaccgc atcgaccgtg aaatctcagc agatgccgac atcctcatcg acgccggcgg
     8641 caaagcgctt ctgccgggca tgatcgactg ccatgtccac ttccgcgaac cgggactgac
     8701 ccataaaggc gacatgcaga gcgaatcgcg cgccgccgcc gccggaggcg tcacctctgt
     8761 catggaaatg ccgaacacca gcccgccggc cgtcacgaac ggacggctcg aagaaaaatt
     8821 cgccatcgcc gcgcagaaaa tggccgtcaa ccattccttc tacctcggcg catcgctcca
     8881 caacctcgac cagattaaag ccgtcgatcc gaaaaatgtc tgcggcgtta aagtttatat
     8941 gggctcatcg accggcgaca tgctggtcga tcatctccag acgctggaag aacttttccg
     9001 cgaatgtccg gtcaacctca ccgctcactg cgaagacgcc ggcatcatca aaaagcatga
     9061 agaaatcttc cgcgaactct acggcgataa aactccgttt tccgcccacc cgcacatccg
     9121 cagcgaagaa gcctgctttt gctccacgca gctcgcagtc gacctggccc gccggcacgg
     9181 cacacgcctg cacgttctgc atatcagcac cgcccgggaa ctcgaactgt tcgacggcgg
     9241 aaccgtgcgc gtaagcagtc cgcgcgaaaa tgaatgtttc atagaacgcg acgaaaccgt
     9301 ccggaaaatc accgccgaag tctgcgttca ttatctccat ttcaccagca gcgtctacga
     9361 aaccaaaggc gcgctggcga aatgcaatcc ggcgatcaaa cgggcttccg accgcgatgc
     9421 cctgctgcgc ggactgctca ccgaccggat cgacaccatc ggcaccgacc acgctccgca
     9481 cacctggcag gaaaaacagg gaacctactg gacagctccg tccggcatgc cggtcattca
     9541 gtccgccctg cccgccgttc tcgaatactt ccacagcgga ctgcttccat tggaaaccat
     9601 cgcggccaag atcgcccata atccggcgcg tattttcaat gtgaaagagc gcggattcat
     9661 ccgcgaaggt tattgggccg atctcgtact ggttgacctg aaccgaccac agaccgtcag
     9721 caaagaaacc atcctctaca aatgcggctg gtcgccgttc gaaggagaga ccttccgttc
     9781 atctgtcgat accaccattg tttccggcca gatcgcctgg cagaacggaa aaataaacga
     9841 cgctgtccgc gggcggcgtt tggaattctg ccggtgaact gcggctcttt tctgtacgcc
     9901 actagtgaac catctgccac gacttgatcg cttcaaccgg atagagcagc atcagcacat
     9961 tcagcgtcag gttatcccgt atccagaaca gcaacaccag ttccgttgcc gcaaacaaaa
    10021 cggcggaacg ccaaaatccg atccgccggg caatgaaaaa tcccaaggcg cagcaggtaa
    10081 tatcgctgag cgagttggcg attgaatcgc ctgtataacc gaggcctatg gttgtttcgc
    10141 gatagcgctg gattacaaat gaactgttct caagaatctc ccacaggcat tcaaccagaa
    10201 ccgcgcacca aagacgccaa atcagacgaa gcctcggaaa tacaagccgg atcaaaccgt
    10261 aaaaaataac gccatgcagg atgtgcgtaa aactgtacgg atcagccaga tgctgcgaag
    10321 tgtgcgcgct ccggatatct ccatcccaca gaaacggtcg agcgcaggca caccaccaga
    10381 tgcggccctg attatgcaac tgaagaaccg taacgctcag cagtgctgca aaagcagctc
    10441 ctgtcagcag tctgtcttta tatcgcagga aacggttcac catgcatcac ccatctgaaa
    10501 cggacagatt ctgccacccg ggcagagtga gaagatgctc aagaagaacg gcgcaggcaa
    10561 aggcgtcgta gagagcatcg tgggcttcgc cgtgcggaca gagttcgcgg acgcgggctt
    10621 cgagttttaa tccgctgatc aaatcctcca gcttatgact cggggccttc ggccatgcct
    10681 gccgggccag tgccagcgta tccacccagg gcccgaaatg atgcatcggc gccatctgcc
    10741 gcagaaattt cttttcggtc gcaacattgt gcgcggcgag cggacgtccg gctacccgcc
    10801 cttccagctt tttccagact ccggacactt catcagcggc ggcgatttca tcgcgcagtt
    10861 tatggtgctt gcccggcgcg taagcgttga acggccggtt actctccaca cggacaaggc
    10921 ttttaaaagt ctgctgcgga tcgacgcgtc cgttttgcag aacaaccgcc gcaacctgcc
    10981 acggttgagt atcgaacccc ggcacggaac cggtggtttc gaagtcgaga acggtcagcg
    11041 ttgtttcgcg gatgagcatg aatcagtcga tccactggat gacggttttc ccgtcgtgac
    11101 cgtgaacggc cttgagttca tcaaggttgt caaaaatggt gatggagcag tagcgcgaca
    11161 gccaggcgag cgcgcattta ccgttgtcga agagaactcc ttcggcaatg tatccggttc
    11221 cggacactcc ggtttcatct tcacggcgat ccagtacgaa gcgtttcatg gcattccttc
    11281 cgttcagtgc agaaactaaa acaaaaaagc ccgctgatga acagcgggct ttttcccaaa
    11341 ccttgaaaaa ggtttgttta gcgcgagtag aactcgacga ccagtgaagc ttcgcactgc
    11401 accggcactt cgccgtgttc cggcaggcgg ctcaggctgg cggtgagctt cttgtcgtcc
    11461 acggtcagat aagccggggt gctggcaatc tgctgggcgg acgggaagat ttccaattca
    11521 cggctgcctt cacggacggt gatgacatcg ccgatacgca tctggtagga aggaatatta
    11581 accttcttgc cgttgatgag aaaatgggca tgaccgacca gctggcgggc ctgatagatg
    11641 gagcgggcga aaccggcacg aagcacaacg gcatcaaaac gggtttcaag cagctgaagc
    11701 atgatgtcag ccgtgttgcc gactttacgc gcagctttgg cgtagtaacc gcgcagctga
    11761 cgttcggtca ggttgtactg gtactgcaaa cgctgttttt cgatgagctg tttaccgtag
    11821 tccgtcagtt tgttacggcg tttcgagggg ccgtgctgtc ccggaggatg cgggcggcgt
    11881 tcgaggaatt tttcacattt cggcgacagg gcaatgccca ggcggcgcgc tttcttgatc
    11941 tttggtccgt tatacttcat ggttctctcc gttttggttt ctgtttcctg cggttccgcc
    12001 caattaagcc cgttttcggg gccgcctgtt tcggaaaact catttcaggc gatcactttg
    12061 tgataatcct ccggcggaac aaggggcgca ttaaagcgat gagaggccgc gaagtcaacc
    12121 tctagaaacg tttttttgcc ttgccttcaa aaacaaagtt cccatcatgt tgcgcagttt
    12181 tcaaccctga ctgaaaagga tttacataat gaaagaagtg aagaaagttg ccattctcac
    12241 cgcaggcggc ctggccccct gcctctcctc cgcaatcggc ggtctgatcg aacgctacac
    12301 agcggtcgcc ccgaaagtga aaatcatctg ctataccagc ggctataaag gcctgctgct
    12361 cggcgaatca attaccgtta ctccggcaat ccgtaccaaa gcatcggtgt tgcacaagca
    12421 cggcggaagc ccgatcggca acagccgcgt caaactcacc aacgtcaaag actgcgtgaa
    12481 gcgcggactc gtcaaagaag gtgaaaatcc gctggaagtc gcggcccgcc agctggtgaa
    12541 agacggcgta gatgtcctcc acaccatcgg cggcgacgac accaatacca ccgcagccga
    12601 cctcgccgcc tatcttaaag acaacaacta cgacctgacg gtcgtcggga tgccgaaaac
    12661 catcgacaat gatgtattcc cgatccgcca gagcctcggc gcatggaccg ccgccgaaga
    12721 aggcgcgaag tattttgaaa acgtcgtggc tgaacacaac gcaaacccgc gtatgctgat
    12781 cattcatgaa gtcatgggcc gcaactgcgg ctggctgacg gcctataccg cgcaggtcta
    12841 ccgtgaccgt ctggcaaaaa tggaattcgt tcccggtatc ggtctgagca aaatccgcaa
    12901 ggatgtccac gcgatcttcg ttcctgaaat ggcgatcgac ttcaaggccg aagccgcccg
    12961 cctgaaaaag atcatggatg agaatgactg cgtgaacatc ttcatctccg aaggcgcagg
    13021 cgttgaagaa atcgttgcgc aaatgcaggc cgccggcgaa gagattgcgc gcgatgcctt
    13081 cggccacgtc aaactcgaca ccatcaaccc cggcaaatgg ttcggcaagc agtttgccga
    13141 aatgctcaag gccgagaagg tactggtgca gaagagcggc tatttcgccc gcgccgccgc
    13201 cgcaaacaag gaagacctca agctgatcaa gcgctgtgcc aacaaggccg ttgaatgcgc
    13261 gctcaaacgc gtcagcggag tgattggcga agacgaagac cagaaagacg tactgcgcgc
    13321 catcgagttc ccgcgcatca aaggcggcaa gccgttcgac atcgacgtca aatggttcgg
    13381 caaactgctg gccggtatcg gccaggccaa agggccgaaa gtcgccgtta aacattaacc
    13441 atcccgcagg gcggcagaaa tgccgccctg ttttgtaatc agccggccgc atgaaaacag
    13501 cgcctttcaa aatctgtccg atgtgctcca aacactggga aacgctggaa acgtttctgt
    13561 ccgacccgga actggcgctg gccggctacc agatcaactt cgccaatcta aagggcggac
    13621 tgttttattt cagccatata aaaaccggat gcggaacaac catggttatt ccggtcggcg
    13681 aatttaccgg gctgagcaac cgtccgatgc ttgcctcgcg gggaaaacag ccggactgct
    13741 gcccggagct ttgtgtccgt aaaaatgagc tggatccctg ccccgtcgaa tgtgaatgcg
    13801 tttgggtgcg agaaatcatg caggtcatcc gtaactggga aaagcgttcc gcttaaatcc
    13861 ccggcattcc gtcggcggat gtttccggca gcgtctcctg ccgttcgatc agttcgatca
    13921 gctgccagcg ttcagaccgg aagcgcgcaa ccatgccgaa agccagcaga cagatataaa
    13981 tcagcgccca gccccaggcc gccaagacac cccacttgaa taccagcacg atcagcagtt
    14041 cgcccgccgc aaagaaaaac cacgccatcg aggtttcgta ccacatcaca aagtgcgtgt
    14101 cgcccgcgcc gcgcagcgcg ccggaaataa tcagattggt ggcgtccatc agtccccagc
    14161 aggcggcgaa taagagcagt gtgcgggcca gtccggaaat ctcggcgaac ggaatgtcgc
    14221 cgcgcgcgaa gagatgaata taaatttcgg gcgtcagcac aaaggtcagc gcgatacaga
    14281 cggtgtaaat ccagccgata tgcactgccc gccagccgac ggcggaagcg ccgtccagat
    14341 cgccgcgccc gatgtgctgc ccgaccagtg tgccggccgc ctggccgatg ccgatggacg
    14401 gcataaaggc gatcatattg acgctcagcg caatgttcgc cgccatgaat gccgtttcgc
    14461 cgagccggcc gatcagcagt acaaacaggg aaaatgcgcc gatatccaga gccatgtgta
    14521 tgccggccgg aacaccgaag cgcagaaggc ggcggaaaag cggcgcatcg aaatagaaaa
    14581 gacggcgggt atcatacagc cggttgtttt tcggcgagaa gtacagcgcc agcataatta
    14641 ccggtgaaac aaaaccggaa atcaccgagg ccagcgccgc gccggccatg cccatttccg
    14701 gaaacggtcc gacgccgaaa atcagcagcc agttcagcag cagattcaag gcggcaccgg
    14761 caatcgcgca aagcatcacc actttggtca gccctcgccc ggaaaaaaaa ctgctgacgg
    14821 ccgaagaaag cggaaactga acaccgccca gcatcagaat gaaaaaatag gttttctcct
    14881 cgatcagtac agccggtgaa tgcccgctga cagacagcat ccagagcccc gccggaatca
    14941 gcagcagaat cagcggaaaa gaaaagagcg ccagcagaat cgcctgcgcc acggagcggc
    15001 tgcaaccgtg ataatcttcg ttgccgtaat actgcgccac cagactgttg gcgaatgccg
    15061 ccgtcgccat aaatccgcac accagtgtga aaaacaggat gcccgccggc acagccgcct
    15121 gaagtgtctc cggactgtac caggccagaa acatgcgggc gcagaagttc tgcaccataa
    15181 atgacgttga agtaatgatc aacggaacgg cgacacgcag cagtgcgcgg tatgaggagg
    15241 atgaagatgg gtgaatggag ttcattaagc ggttcagtgt ttccgaaccc ggcaaaaatt
    15301 gcgagctgtc ttttaccgcg ttcctcaacc ggcccggtaa agtcgatcag gcgcccattt
    15361 ggcggcggaa ggtcagctcc ggcacttcat ttgtttcggc agccggcagc tccatcagcc
    15421 gactgacacg ttcggtcaac tccggaataa tgatatccgg cgaaccttcg aacagttcga
    15481 agcaggcgaa ggtttccgca tcaatccggc tgctcagcgc gttcagcgat ttggcgtacg
    15541 gactggtatt ggcgagtttg ccgtcgaaga ccagaaaaac gcgggtgaaa tggccgctct
    15601 tgagttcatt aaccagcgaa cgcaggaata cattgaagcc gtcgccggtg tcgcccatga
    15661 agcgatcagt tatgatgtcg ttcgtgcaga atgtgcagac cgccactttt tcgttgttaa
    15721 caacaaatgc gccgtcgacc agatcatcac cgccggtgcg atactggcct tctccgattt
    15781 tccgggcgag aacgcccgga tagttggcca caccgagaag aacgatatct ttgaactcgc
    15841 cgtatttttt gtcggccttt tcgcgcattt tatcggcaac cgccgcaacc tgcggttccg
    15901 gctcaggagc agaaatgaca gcagccggct cggcggcaga tacaggtttt gcagcaggcg
    15961 ggttctccgt cacaaccgga tttcccggca gttccttttc agagattttc aacccggcac
    16021 gaaggctcaa tgcgcgttca acggcttcgt tgacatcggc gcgcaataca tcgcgcgagg
    16081 gcagcttttc ctggatttca ccggccagca tttcaaaatt ttcatagagg gtgtcggcga
    16141 tttcttcgcc gatgcgcgca acggaggatt ccatgcgttc aattgcagac acggttttct
    16201 caaccatcgc ggcctgcatg gcacttgtct cttccgattt tttcgcgata gagagctcgg
    16261 ccatccggca gagtgcaatc gcggcgattg ccagaacgat tgcggaaacg gaaagtccca
    16321 gcgtcaaaag aaaactcatc agaccggttg ttgaaatggt ttcaacgccg ccctgcatgg
    16381 cgtgatacac agaaacggct gccgctgcgc cgagaatggc cgaaccggta atgatgccga
    16441 tggttcccac ggctgcacct ttagtgtgca tgatcacttc cggttttgtt ctttcgcctg
    16501 ggtccataaa cggccttcac tggtgaactt taaacttttg ccgcgcatta tatgagccgc
    16561 cccgtcgaag ccaagagatt tatcggcaaa accggaactg atacatgccc ggtcagatgc
    16621 gtaatccgtc agctcggcac gccgccggtg aaaatatcaa atgccgacac agcgtttttc
    16681 gcctcgataa gcgctttgcg catcgactga tctttcagcc gcgaactgat cgctgaaagc
    16741 gcgcggatgt attgcggatg ctgcgtgccg gacgccgcca ccatacaaac gatttgaacc
    16801 ggaatttcgt ccagcgagga atagtcatcc agcggttggc gcgcaatgcc gagagccatc
    16861 accaggtcat ctacggaggc aagcctcaca tgcggcacgc cgacaccgaa tccgattccg
    16921 gtgctcatca gttcttcgcg tttgaaaatt gcctgctcca gctcggcttc gttatgaatc
    16981 agcggagagg ttcccagcgc ggcgcatagg gttttcagcg cggctgtttt ttcattggta
    17041 tcaaagaaaa ccacccgttc cggcgaaaga atccggctca gcgtttccag acccggcgcg
    17101 acaggagccg gagcggcaga gctcccgata cagcggttca cccaaccctc aatatcactg
    17161 cgcttaaagc gccaggctgt acccagcttg ccgcccggca actgcccctt ctgcgcccag
    17221 tcataaaccg tccgttccga tacgcgcaga tatgatgcaa cctcttcgat ggtcataatt
    17281 tcatggttca tgcaagagcc ctcaatgtaa cgcgacgcat tatatgcaaa atctgcaact
    17341 atctgcaatt aaacagatgc aaaaaattgc tccgaattgg taaaagttag cgccgccagc
    17401 ttttcaaccg gaatgccgcg cagttccgca atagcgcgga cgacaactgc cagattggcc
    17461 ggttcgttgg ggggcaggca gcccgccagc ggaaagtcag gtgaatcagt ttcgatcagc
    17521 aaacggccgt tcggaactgc cgctgcagac gccctcaccc gcttcgactg tggattcgcc
    17581 gccgatccgc agaacgaaaa ccagcagttc agttcaacca gttccggaat cagttcagcc
    17641 gctccgccga aagcgtgcag taaaacgcgt ggtgcgggat ggacacgtaa aatttcaacc
    17701 agccggcccc aggcgcgaac gcaatgaacc gcaacaggcc ggttcagttc gcgcgccata
    17761 tccaggtgcg cagcaaagca ttcttcctgt tcaacgcggt tagtacattt ttcttgaaaa
    17821 tccaaccctg tctcgccgac gcccgcttcc ggatgttcct ccagcagttt ttccagcatc
    17881 tggacgtgtt tcagccaacc agttgaaaca aaccacggat ggattccaac catcggaatg
    17941 acttgaggaa actgctccgc aagttgaaca accgtctgcc agtcgccgga gcttgtcccg
    18001 cagcagacaa accgttcaac gcctgctgcc gccgcgcgtt ccatgacatt tttaatatcc
    18061 ggaaacagcg cctgatcctg caggtgacaa tggacatcga acagcttcat gaacgacaaa
    18121 atacaagccg cttatcgaac aggcaagccc ttagaaaatc ctttatttgg tggctgttta
    18181 taactttata cacaacatat tgtatttttt acttgccgtt tgaatcaacc attacgataa
    18241 atatgatcat tctggtaggt aataacgtaa caagaggcac tgatgaatat taaaattgaa
    18301 acttcccgcg acagcctgct gtctgaattc gggctcaaaa cgctcgaaag ccgctatatg
    18361 ctcccgaccg aaaaaagccc gcaggaggcg tttgcccgcg ccgccgccgc tttttcggac
    18421 actccggaaa tggcccagcg gatttacgac tatgccagca tgctctggtt catgttcgcc
    18481 acgccgatcc tgaccaacgg cggaaccaac cgcggactgc cgatctcctg cttcctgaac
    18541 tatgtgcccg acagccgcgg cggaatcaca ggccactaca ccgaaaccgc ctggctttcc
    18601 tccttcggcg gcggcgtcgg cggctactgg ggcgacgtgc gtgcctgcgg caccagaacc
    18661 tcgcacggct ccgagtccac cggcgtcatt cctttcctga aagtggtcga tgccgaaatg
    18721 ctggcgttca gccagggcgt gacgcggcgc ggttcttacg cggcctatct gccggtcgac
    18781 catccggaaa ttgaagaatt cctcgacgtg cgcaagccga ccggcggcga cgtaaaccgc
    18841 aaatcgacca acctgcacca cggcgtcgtt ctgaccgaag agttcatgaa tctgattgat
    18901 caggcgacgc gcattcccgg cttcgacgac agcttcaacc tcgtcgatcc gcacaccaaa
    18961 gaggtgaaga aaacggtgtc ggccaaaacc atctgggtga agctgatcca gaaccggatt
    19021 gaaaccggcg agccctatat tctgttcgac aaagccgtca acgatgccct gcccgaattc
    19081 cagaaaaagc tcggcctgcg cgttcatcag tccaacctgt gcagcgaaat caccctgccg
    19141 accaacgcag aacgcacggc ggtctgctgt ctgagttccg tcaacctgga aacctacgac
    19201 gagtggaaag atcatccgca tttcatttca gacctcgtcc gctttctcga caacgtgatt
    19261 caatcgttta tcgataatgc gccggacgaa atcggccgcg cccggttttc ggcgcagcgc
    19321 gagcggagta tcgggctcgg cgcgatgggt tttcacgcct acctgcaggc gcacagcatt
    19381 ccgttcgaaa gcgccattgc caaggggctc aacctgaata tgtttaaaca catcaaagag
    19441 caggcttcgg cggcaacgca gaagctggcg gaagagcgcg gcgaatgccc ggacgccgcc
    19501 ggctacggcg tacgcaacgc acacctgctt tcgattgcgc cgaacgccag cagctccatc
    19561 atctgcggca ataccagccc gtcgattgag ccgttccgcg ccaacgcctt cacgcagaaa
    19621 accaagagcg gctcggcgct gatgaaaaat aaatacctcg aagaggcgct cgaacgccac
    19681 ggacagaata attccgaagt ctggaaatcg atcatcacgc agggcggcag cgcgcagcac
    19741 ctcgactttc tgaccgactg ggagcgcgac gttttcaaaa ccgcgctgga aatcgatcag
    19801 cgctggatca ttgaatttgc cgctgaccgg cagcagtacg tctgtcagtc gcagagtgta
    19861 aaccttttct tccccgccaa tgtcagcaag caggaactgc ataacaccca catgctggcc
    19921 tggaaaaaag gattgaaaag cctctactac gtccgcagcg aagccattaa acgcgccgat
    19981 gtcgtgtcgg agcagaagcc gcgcgaaatc atgctcgatt acaataaaga aaccacctgc
    20041 ctttcgtgtg aaggataatt taatacagcg tactgcgtat tccgtatctc tcatcacacc
    20101 ggcgcaatat gcagtatgca ctacgcaata taaaacaagg agatccccat gccgctttta
    20161 gaagaaagaa cttactacaa accgtttgaa tacgactggg cgtttgaagc ctacaaaatg
    20221 cagcagcaga tgcactggat tcccgacgag gtgaacctcg ccgacgacct caaagatttc
    20281 aacgaaaaca ttccgcagaa agataaaaac ctgctactga atattttcaa attcttcacg
    20341 caggccgacg tcgacgtcgc cggaggctac ggcacgctct tcctgccgac cttcaaaatt
    20401 ccggaagtcc gcatgatgct cagcgccttc gccgccatgg aagcggttca tcaggatgct
    20461 tattcgctgc tgctcgaaac gctcggtctc agcgacgacg aatacaccgc tttcatgtcg
    20521 attgccgaga tgaaggaaaa gcatgaatat ctgcttaact tcaacatgaa cacgccgttc
    20581 gaaattgcca aaacactcgc cgtttacagc gctttcacgg aaggtatcca gcttttcagc
    20641 agcttcgcca tcctgatgaa ctttccgcgc ttcaacaaaa tgaaaggcat ggggcagatt
    20701 gtcacctgga gcatccgcga cgaaagcctg cacgtcgaat caatggccct gctcttcaaa
    20761 gccttcatca aagagcatcc ggaactctgg accgataaac tgaaatacga aatctactgc
    20821 gccgccgaac gctccgttga actcgaagac aacttcatcg acatcgcctt tgaactcggc
    20881 gcggtcgaag gcctcacgcc ggacgaagtc aaacagtaca tccgctacat cgccaaccgc
    20941 cggctcatcg gcatcggcat gaaaagcatc tacaccgtac cggaaaatcc cctgccgtgg
    21001 ctcgactaca tgctcaacgg cgtcgaacac accaacttct tcgaaaaccg ctccaccgaa
    21061 tacgccaaag cgtcaacgac gggaaactgg caggacattt ttaagtaagg gatatgggcg
    21121 tgtgtgagta tgggggtgcg tgggtttggg cgtgtgtgag tctctaaacc aacctaccca
    21181 caaactcaca gactcactta cccacaaact caccgtcttc cgccccgtcg gttctggtag
    21241 ttctcttcgg cttccgcctt catctctgca atcagctcaa cggtcaacgg catcggatct
    21301 ttcggaacca ccccgttcgc gaaaccgcga tcccggggat ccaagtcgaa ttcctccccg
    21361 cgggctttcg gcatctgttt gaagcggtac agataaaaat tctcgacctt atgccgggcc
    21421 cagtccgtct tccgcaaaaa tttaacggcg ctggagatgg agggattcat gcgaaaacag
    21481 ttgagcccca gtgcggcgta taaaatttcc cacccgtaaa actccacgag tgcagtcacc
    21541 atcgcctcag cgctcaaccc atgcaacggg ttatttttct tttcttcaac ggacataaaa
    21601 ctcctccacg aatcggcgcc gattatacag agttcttgtt tagaccggac tgtttaagcc
    21661 aactatttaa ttgggattaa ccagcgttat aaattcccca aagcgggctg aaagcagagg
    21721 cgtgatagag gccaaaattc agtcgttctc aataatcaac cgtaacgcca gattggcctg
    21781 catggaagcg acaatgccga cgcgcggagc catgaggccg caaccgggct gcgccgcagt
    21841 ttcaagatcg ccgacgatat acacgttgcg gcccattttt ttgacgccga tggtttcttc
    21901 cggtccgaag ccggccatgc cggaagcacc gatgaacggt acgcccggtt tctgcgcggt
    21961 gaacgcctgt aagagcattg ctttctgatc cgcgcgatcg aaggcttcga tcatcacatc
    22021 gacggctcca aaggttggaa acaggttgtc cggcgtgagc ttcacggcgt gagcttcgac
    22081 ctgaacatac ggattgattt tccgaaggtt ggaaaccagc gcatcggttt tgagcattcc
    22141 gatctgatct acaaagtatt gctggcggtt gaggttgctc ggctccacta cgtcaaagtc
    22201 ggcaacaatc agtttgccga cgccggtgcg cgccagagcg acggcgatgg ccgagccgag
    22261 gccgccgagc ccggcgatgc cgacggtcgc agccttcagc ctggcatgta cgccgggcgt
    22321 atggcgcgaa gccatcagcg cttccagttc gtccgccgac ggcgtttcgc cgcgacggat
    22381 caacgaaact tcatcgccgg ctttgagcag aacgtcagcg gtcatcgccg cgccgttgag
    22441 aatgaccaca tcggcggaag gcttcacggc gtcgcgcagc gcgaagagcg tttttgcacc
    22501 ggtttcaacc ggacgttcat taagtcgaat attcatactg agcattaacc atgtttacgg
    22561 gaagtttcga cagaaggaca cgaaggaaaa cgaagggaga atccggtaaa aagagccctt
    22621 cgttaaccgt tgttcccttc tgtaaattta tcttctatct gctgttgaga cagttccttt
    22681 ccggcaggtg cggagaatga tttcacgatc atgtggatac cggtcaaaat aatgaaaacg
    22741 gcaaatatcc gcttgaggac gatatcggaa ataccgaaga caagatgtgt tccgagcatc
    22801 gctccggcaa taccgccaag cgccatcagg ccgcaaaccg gcaggttgat cggaacatca
    22861 ggattcagct tatagcgaat ggctccggcc agcgctatgg gcagcatgat gaaaagcgac
    22921 atcccctgcg cgcttttctg cccgaagctg aatccaagcg ccagaatcgg gacgattaaa
    22981 acgccgccgc cgacaccgaa cagggagctc ataatgccgg cagccagtcc ggcgatgata
    23041 tacagatacc agttttccat ttattccttc ctttcaaaaa accgtttcaa aaactccagt
    23101 ttgcgcacgc gatgttccag cctgccgcct tcgtgcccgg cgtgcgcata gacggccatt
    23161 tctttaaccg cgcggatttt attataggcc gcataggtac actccggcgg acagactgta
    23221 tccttcagac cgcacgaaac cagtaccgga caggtaatgt ccggcgcgag gttgatgttg
    23281 tcgaagtacg acagattccg gagaaccgtt tcgatccgtt caggaaacat cgccagatac
    23341 ttcgcaattc ccgcgccgcc gccgctgcgg gttgcaatcc ggtgttccat ccggcagctg
    23401 ctgggcacat ccgccatgca aagcgacacg gaacagtcaa gcgccgccag ccccagcgcc
    23461 attccgccgc cctgactgtg tccttccgcc gcaatccgtt ccggatcgat ctccggagtt
    23521 tcgcgtgcca gccggacggc gcgcaggaaa tcggtccaga cgcagtaaag atagctgttc
    23581 atcaggtcgt tgagattcaa cgtccaccag ccggacttac cgtcgccgtc aaacgccgta
    23641 actgaaccgg tcgtaccgca ctgaagtcgg aagtccggtg aaatcacagc acagcccgcc
    23701 gccagccagt gtgaaaaatc atccggcgct ccgcggctgc ggtttgcgcc gtgacagtaa
    23761 ataacaaccg gaactttatg atccttcgct tcaggcggga gcagcagcca ggcatgaatc
    23821 ggcgtcccgt cgagtccgtc aaacgtcagg tcgcgaatat caggcgaaga atccgtgctg
    23881 agttttacgt tcagcggaac ctcttcgcac cgccggcgcg cggccagcca gaacgtatcc
    23941 atatccggtt gcgcggtctg cgggacattc accgttgcga gctgatcggt ttgagacatg
    24001 cggctttctt ctttccttca ctccggaggc ttcttagaat ccgcgctatg aaagcatatc
    24061 gtccgtttat tgtcatcctg ctcgccgccc tcattttacc aggggcgggc aactcccaga
    24121 gcatttctgc agacaccggg aaaaagccgc tcgtgtacca gattccgatt caggatcaga
    24181 tcgagcccgc cctgctctac gtcgtccgcc gcggcgtcgc cgaagccgcc cgcgaaaaag
    24241 ccgccgccat tgtctttgtc atgaacacgc ccggcggcgg tgtgaaagaa gcgcgcgaaa
    24301 tcatcagcct gatcgggcag attgacgtac cggtttacac cttcgtcgaa aaagacgctt
    24361 actctgccgg cgccatcatc gcgctctcca caaagcacat ttacatggcc cccggcagcg
    24421 tcatcggagc ggccaccccg ctggtaatga gtccgttcgg cggagtcgaa aagatgccgg
    24481 acgcaatcga agaaaaaatg acatccgccg tcgccgcact ggtgcgagcc gccgccgaac
    24541 agggcggaca cgatccgcag cttggcgaag ccatggtccg gcgcgagctc ggctacacgg
    24601 ctggaaccaa tgtgctgagc aaagccggag aactgctcac gctgaccgac agcgaagcca
    24661 gaggtattct ttccgaaggc accgtggccg acgtaccggc gatgcttaaa gccgccggac
    24721 tgccggacgc ggaaattaaa acgctcgaag tcacgcctgc cgaaaaaatc gcgcgcttca
    24781 tcgccgcctt tgcaccgatt ctcctgatga tcggactcgg cggaatctac attgaaatca
    24841 aaacgccggg ctttggactg ccgggtattc tcggcacgac cgcactggtt ctattcttct
    24901 tcggccacca catcgccgga ctggcgggca tggaagacat cattctcttc atcgtcggct
    24961 tcagtctgct tcttgtggaa atattcatcc tgcccggaca tatcgtattc ggcgtcagcg
    25021 gtatcgggct cattctgatt tcgctgctca acgccatgac ctggaaaatt ccgggacact
    25081 tctggccttc gctcagcgac agcacatggc actacgcaat cggcaatctg gcgctgggtc
    25141 tgacaggtac cgttgcgctt ggaatttttg ccggaaaata cctgccgaaa tcgcgggtcc
    25201 tccgcccgat ggtgctgagc cagcagacga atcgcgccgc gggctttacg gcggcgcatg
    25261 accgcagcga cctgcttggc aaagaaggta tcgccgaaat gaacctgcgt ccggcaggcc
    25321 gcgcgctttt cggaaacgac cgcgtaaacg tcatcgcccg cggtgagttt attgaaaaag
    25381 gttccgccgt gcgcgtgatc gaaacctccg gcagccg
//
LOCUS       DAOPZO010000014        22697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_2302_length_22697_cov_8.745076, whole genome shotgun sequence.
ACCESSION   DAOPZO010000014 DAOPZO010000000
VERSION     DAOPZO010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 22697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22697
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2302_length_22697_cov_8.745076"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="PKI68_02075"
     CDS             complement(<1..1031)
                     /locus_tag="PKI68_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNX52512.1"
                     /translation="MSRHYLAVDLGASSGRTIVGTLENGLLTLKEMNRFWNGPTEING
                     TLYWDFIHLFRNIQEGIALAKKEFGGGLVSIGIDTWGVDFGLIDAQGKLLGNPVNYRD
                     SRTNGMPEKVFAAVGKETVFNQTGIQFMQLNSLYQIYALAQSNDTQYLQADKLLFAPD
                     LLNYWLTGRRATNRTFASTSQMYNPVAKDWAFDLLAKLNIRTDLFAPFADPGTVLGDY
                     QGLPVVNVGSHDTASAFAAVPVAEGEQCAFLSSGTWSLLGTELPAPVINGNVLAANFT
                     NEVGVCGTIRFLKNLSGLWMMQEIRRTWAEQGFTYTWDEMAELALRSVPFEFFINPGD
                     DVFLPPGDMP"
     gene            complement(1049..2833)
                     /locus_tag="PKI68_02080"
     CDS             complement(1049..2833)
                     /locus_tag="PKI68_02080"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HNX52513.1"
                     /translation="MAEKTWITALPKIGIRPTIDGRYGGVRESLEGQVMTMARSAAKL
                     ISSSLKYPNGKPVECVIADTCIGGVAEAAACAEKFNRENVGVSLTVTPCWCYGSETMD
                     MDPLRPKAVWGFNGTERPGAVYLAAVLAGHTQKGLPAFGIYGRDVQDAGDTSIPADVE
                     EKILRFARAGLAVAMMKGKSYLSMGGCSMGIAGSIVDQNFFEEYLGMRVEAIDLTEFT
                     RRMRDGIYDQAEYEKALAWVKENCREGRDPNGAATKRSRKQLNREWEDSVKMALITRD
                     LMVGSDKLDKMGYKEEARGHNAILSGFQGQRNWTDCSPNGDFLEAVSNSSFDWHGIRM
                     PRLVATENDSLNGVPMLFAYLLTSTAQVFADVRTYWSPEAIRRVTGYEVEGAAAGGLI
                     HLINSGSAALDGSGRQSRDGLPAMKPFWEISRKEAKACLDATTWHPAVTEYFPGGGWS
                     SKFVTQGGMPITMSRVNLVKGLGPVLQIAEGWTVELPEKVHNVLDNRTNSTWPTTWFA
                     PRITGKGAFTDVYEVMAKWGANHGAFSCGHIGADLITMASMLRIPVFMHNVEDEKVYR
                     PSSWSSFGTEGLAGADFRACQTYGPVYG"
     gene            2940..3866
                     /locus_tag="PKI68_02085"
     CDS             2940..3866
                     /locus_tag="PKI68_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNX52514.1"
                     /translation="MNEIISYGRFRCKELAPHKNRGMEITYIEKGVLEWMVEGVPEKI
                     EPGSVFFTLPWQVHGSLHPSEPDNVVWHVLFHLDEDYPAPRRQFGFPPGFGFSPIEMK
                     MLSTAFTSGSKHCFRATPAMHWLMPALINELQTTHELAKAHSISLLRAVLVELKRIVS
                     GDAVDTDTHTWSEKKVQALLTDLSSSCDQQWTLKQMAGRCGIRRTRLNTVFQKLTGST
                     PMEYLTRLRMERAKTLLRETGIKIIDIAFECGFGSSQYFANTFKQATGMTPTGYRIHC
                     ARLTTDELLKWKNVKFRSEQEERRRVQNFSAN"
     gene            complement(3940..7101)
                     /gene="lptD"
                     /locus_tag="PKI68_02090"
     CDS             complement(3940..7101)
                     /gene="lptD"
                     /locus_tag="PKI68_02090"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HNX52515.1"
                     /translation="MNLKQNCAFIAGFLAVFTLLAVTGCSTAMPYQETRSGNYFYLNP
                     FAYKPATAEEHWQYAESLKEKGSIRSARKQFEIFVKRWPEDAHAATAKKTVAEIYAEK
                     GKNKKAFTAYEELIQQYYTSITNYNSILDQQYTLAEKEMNRKRMRWLFGGYRAPERAV
                     PLFESIIKNAPQWERAPEMQYMIGQAYQKNNDPELAVVAYATTEYRYPNSPFAEKAAV
                     AKIDSFKTLVDSTPYSIDIREQAELSAGLFPELYTNSQHIAEVHDFSVKLHTLSAEHN
                     YEVGRFYERIPRPPQTNSAAIYYNKVIRKFGDTEYAAKSAERLRVLFPHGEALLADGT
                     KAPIVPVAGTSGESGGGGAAAAGGGEAAAASAGSGSGSVRVEAKPLPERTADDPNAVE
                     VTADRMEYSGKLLIGDGNVAVQQAGTSLQADHVTVNSETGEIKASGNIRMVRDGNYWE
                     GQELAYNYKTQAGTFGKSAMYFEPAYITAETTERVSTNEFLMHNVMMTTCSGEHPAIY
                     AKAKELRIINDKETGVFVKAKGVTFFAGPVPIFYTPYWQRHLDHGVFTCGIGYGGRVG
                     GYIKYRAEVHPTDWLRSVSHFDLYSRRGLGLGQDFRWTTPTGGGGIETYYINDNDPYN
                     GSSNSRHNMGFDKSLIDSQRYRVKITDREKIDEQTYFATKVNWLSDPLVLKDFFNDDF
                     RNEANPENYAVVQRSADDYAASLRVDRRMNDFYTTVDRLPEMTYDWYRSRLGDSPFYF
                     ESENNVAFLQKQNAETNMFPIPRADNYRSARFDTYDRIFLPLRFKEFFNVIPRAGYRG
                     TWYSETAEGANADYRNIVEMGTLTSFKAYKPITEKSSYFGDGLRHVVEPYADYSWRPE
                     PSMTPTNLFQFDSVDALDKQNEVRFGARNLLQTKRGLKRVSNFVDSDIYTTGRFETPE
                     GQKSFSNLVADTELSLTDNFFVQSDLEYNWYTHDVNPANARLKFVSDDKSEYSMEYRY
                     LAGTRSLITPRVKLFPNDDWSYEFSASYDEKFNEWYERKILVNHKFNCIGMGVGFRID
                     EEDKTQFWVQFWLTAFPQGSLKL"
     gene            complement(7170..7733)
                     /locus_tag="PKI68_02095"
     CDS             complement(7170..7733)
                     /locus_tag="PKI68_02095"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNX52516.1"
                     /translation="MKKVWIVLGIILLVVVMIGGSAIGSKNRFVSQDEAVTAQWANVE
                     TVLQRRFDLIPNLISTVKGYAAHEKGLLTEVTRLRSQWGLASTPDEKIKAAGEMQGAL
                     SRLLLVAENYPELKANQNFMALQDELSGTENRISVERRRYNEMVQAYNTSIRRFPGSV
                     WAGMFGFSRRTPFESAPEASVVPRVEF"
     gene            complement(7749..8456)
                     /locus_tag="PKI68_02100"
     CDS             complement(7749..8456)
                     /locus_tag="PKI68_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52517.1"
                     /translation="MKKMSPEQFTEKLKTVCGDNLQAVVLYGSAAAGDYAAKGSDYNL
                     LVVLNDLSPAVLRNLAKPVAVWERAGNPPPLMFTRKRLADAADVFPVELLDMRDARRV
                     LSGEDVIAAIEPGMANLRLQVERELRSAVIQLCRSYLSVSGNPRRLTALLTGSLSGVL
                     TLFRAALRLYENPVPAEKFQALEKLTGHVPVKLESFRRIQALKAGTLKIKNVDAGRLF
                     EEYLASIEAVVDAVDRI"
     gene            complement(8472..9224)
                     /locus_tag="PKI68_02105"
     CDS             complement(8472..9224)
                     /locus_tag="PKI68_02105"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNX52518.1"
                     /translation="MRRFFITVLVLLAGFLPVRADTDRLLNSLKPAGYVSDFANVMNS
                     SDRAAVESTLAELEQKTGAQVAVVTLKSLEGGQIDDFANRLFARWGIGKKGKDNGLLL
                     IAAIEDRKVRIETGYGFEGVLPDAAAGRLIDQYVRPAFRNGDYSGGLRSGAVALASVA
                     ASAAGVELTGVRNQERYSGSQSQDRKGGGLIQLIFFIIIIFAVIRHPWLLLLFMGGGG
                     GRSGGGFGGGSSGGFGGFGGGMSGGGGASRGW"
     regulatory      9378..9486
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            9542..10816
                     /locus_tag="PKI68_02110"
     CDS             9542..10816
                     /locus_tag="PKI68_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNX52519.1"
                     /translation="MSKNTETLALHAGYKPDPTGSCAVPVYRTSAYVFRDTEHAANLF
                     GLKELGNIYTRLMNPTNDVLEQRVAALEGGAGALALASGTAAIFYSIINLAQAGDEIV
                     SSSNLYGGTYTQFNNILPQLGIKVKFVDPSNPQNFAKAITEKTKALYTETIGNPSLDV
                     ADIEAIAKIAHAHGLPLIVDSTFTTPHLLRPIDHGADIVVHSLTKWLGGHGNGIGGIV
                     VDSGKFNWATGKHPLLSEPDESYHGIRYAHDLPPSLAPLAYILRMRLVPLRNLGACLS
                     PDNAWQFLQGIETLALRMERHGENALAVAKFLEKQAEVEWVRYPGLESHPSHKIAKKL
                     LKNGFGATVVFEVKGGEAAGKKFIEKLQIFSHLANVGDAKSLAIHPATTTHSQLTAEQ
                     QAEGGITPGLIRLSVGLEHIDDILADLKQALK"
     gene            10890..12020
                     /locus_tag="PKI68_02115"
     CDS             10890..12020
                     /locus_tag="PKI68_02115"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNX52520.1"
                     /translation="MKTVETQFFTYDALTLQSGVRFGPVTLAYETYGTLNEKRDNAIL
                     LFHALTGSQHAAGVNPAVPGIGERWNAECQNGWWSDYLGPAKVVDTNRFFVICINYFG
                     GCYGSTGPSSINPATGKPYGGKFPRIHFSDIVDSQLPLLKHLRIEKLHAVIGASLGGM
                     LLANLAARYPHITDRLIAIACGIEPTTLIRAHNIEQILAIENDPHFCNGDYYDGEPPV
                     RGLALARMISHKTFVSLDFIEERAKNRWEQEYDEFSWYKVRTPLESYILHQGRKLVRR
                     FDANTYLQLIAAWSDYHLLRDTGAPDYVTLFKNCRHQRYTLFTIDDDYCFYPEEQEYY
                     RKKLEEGRVPVKHITVHSNKGHDSFLLEPHLYEQAIREALNS"
     gene            12034..13503
                     /gene="cls"
                     /locus_tag="PKI68_02120"
     CDS             12034..13503
                     /gene="cls"
                     /locus_tag="PKI68_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HNX52521.1"
                     /translation="MSQHILWAAGWMFHLTAFALVTFHCLQRRRNASSTLLWIFITWS
                     IPVLGALFYLSFGIDRVDDRGYLKFLTDEHLLKTRKGTRDAAPRAYWHHLTGAPAENE
                     FQREFNRTLDALIPDHPALAGNRLVPLVNGDEAYPRMLEAIRQARHHIHLQSYIIGND
                     SVSREFMAALSAKAAEGVQVRLLYDRFGSTYALFGGLFRKARKTPGFKVAGWTQANLL
                     KRQFQINLRNHRKTLVVDGEKGFFGGINLLDDHISRPGKPPIRDYHFEAEGPIVQELQ
                     YSFLRDWYFITEESPGELLTEEYFPQTKPAGNITARLISSGPSTVEDVAIESFFNSIV
                     LAQKQILAVTPYFVPPADILRALRSAALRGVDVQLVVPKENNHRYAGYASRALYEELL
                     LAGVRIFERRPPFMHAKALIIDGEFALVGTANLDVRSLSLNYETCLAVYSAGFVDVMK
                     RIIHEDIACSDEVLLADWQSRPTRYRLLENLASLMTPIL"
     gene            complement(13750..15165)
                     /gene="uxaC"
                     /locus_tag="PKI68_02125"
     CDS             complement(13750..15165)
                     /gene="uxaC"
                     /locus_tag="PKI68_02125"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF002794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HNX52522.1"
                     /translation="MKSFMDRDFLLQTKTAEVLYHSHAAKMPIYDYHCHLSPREIAED
                     RRYDNLGEIWLGGDHYKWRAMRTNGVTEEFVTGSAPWRVKFGKWAETVPQTIRNPLYH
                     WTHLELQRYFGIDTLLSPETAEQIFETATAQLRTPEFSARNLMRKMNVKLVCTTDDPV
                     DSLEYHKQIAADRFEIKVLPTFRPDKAMNLSDVTVWKKYIQTLEKVSGVEIKDFAALV
                     AAVEKRHEFFHAAGCRLSDHGVAYVPDAQASSMELNIIFQKAMNGDRVTLDEREKFEA
                     VFLYEVGKMNHSRNWTQQFHVGVFRNNNSRLFSKIGPDTGFDSIADYRQGPGLIRLLD
                     RLDSSNQLAKTILYNINPCDNELMATMIGNYQDGSFPGKMQFGSGWWFLDQKDGMEKQ
                     MNALSSLGLLSRFIGMLTDSRSFLSYPRHEYFRRILCNLIGNDVENGEIPADMDLLGT
                     MVENICFNNAKNYFGIDLQDG"
     gene            complement(15162..16451)
                     /locus_tag="PKI68_02130"
     CDS             complement(15162..16451)
                     /locus_tag="PKI68_02130"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNX52523.1"
                     /translation="MISRFGEEMSISCDELRALVSQILENRPGPLRKVLILPPDHTRL
                     NSQAGPITAIAYELLTADGVQVDILPTLGTHNAMTEKQLRMMYGETIPLKAFKVHDWR
                     NGIVRKGEVSGEMLAELSGGKVNYSIGVEVNRMLFDGYDLILSVGQVVPHEVVGMANY
                     TKNIVVGAGGSDIINKSHFLGAVCNMETILGQTDTPVRRLFNYAVDTFLGDLPITYIL
                     TVVQQDYDTGKMHLRGFYAGDDVQVYNEACKLARKVNITLLDREPKKVVVYLDPHEFQ
                     STWLGNKSVYRTRMAIADGGELIVLAPALKEFGEDPKIDELIRKYGYRGTPATLKAVK
                     ENEELRSNLSAAAHLIHGSSEGRFSITYCPGPDITLDEVRSVGFNAVPYAEMAAHYNP
                     AELKDGFNRMPDGEEIFYISNPALGLWAYKEKFGEDL"
     gene            complement(16464..17918)
                     /gene="gnd"
                     /locus_tag="PKI68_02135"
     CDS             complement(16464..17918)
                     /gene="gnd"
                     /locus_tag="PKI68_02135"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HNX52524.1"
                     /translation="MKKKADIGLIGLAVMGENLVLNMESKGFTVAVFNRTVEKVDAFT
                     AGRGAGKNFIGCHSIKEFCASLERPRKVMMLVKAGKAVDDFIEQVIPHLEPGDIIIDG
                     GNSHFPDTIRRTAYVESKGLLFIGTGVSGGEEGALLGPSIMPGGSPAAWPAVKPIFQK
                     ISAQTAAGEPCCEWVGENGAGHFVKMVHNGIEYGDMQMICETYQMMKEGLGLSNDEMH
                     EVFTEWNKGELDSYLIEITRDILGYKTKTGEAVIDQILDTAGQKGTGKWTAVAALDLG
                     QPLTLIGEAVFARCLSALKDERVAASKTLKGPGAKFTGDKAALIEDLKQALYASKIVS
                     YAQGYQLMRAAADEFGWKLNNGGIALMWRGGCIIRSVFLGKIKEAFDTNPDLVNLLLA
                     PFFTKAVENAQAGWRRVICKSVELGIPMPAISAALSYYDGYRAARLPANLLQAQRDYF
                     GAHTYERIDKPRGEFFHTNWTGRGGDTAASTYIV"
     gene            complement(17955..19028)
                     /locus_tag="PKI68_02140"
     CDS             complement(17955..19028)
                     /locus_tag="PKI68_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNX52525.1"
                     /translation="MTEKSSKKYSLVIATSMGIRLTPVDSQPFHCSDTFKMQATSAES
                     NVASIASYLGMPVKVLTAFIKGSPVSHFIKDNLRSRRMDFEGPEFDQGGPWGYRHQIN
                     MADSGFGARGPRVHNDRAGEVGRELCADDFDLDRIFAKEGAGIVHLSGLIAALSPKTS
                     KFCLDIARIAKENGSQISFDLNYRASFWKGREKELSAAFAEIASRSDILIGNEEDFQL
                     CLGIKGPEAGGKGLDDKIDGFKEMIGRVQAAYPNATWFGTTLREVVSANSHMWGALVT
                     DGKEWEIIKPREIGVIDRIGGGDGFVGGLLYGILKGWDVKKCARFGWASGALAATMLT
                     DYAQPADEEQLWSIWNGNARVRR"
     gene            19238..20716
                     /gene="uxuA"
                     /locus_tag="PKI68_02145"
     CDS             19238..20716
                     /gene="uxuA"
                     /locus_tag="PKI68_02145"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF003027.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HNX52526.1"
                     /translation="MSEIYNKNWNRERVRGGMRWFGPEDIVTIRNIRQTPGITNVITA
                     LHHIPPGQVWTDAEIAKRKIEVEFWPQSDAPADLEGMPLFRWYQVNGNPTGLVWDTAE
                     SLIFTEDIKNGSPNRDRHIENYQESLRNLGRFGVCNVIGNFMLVADWTRTGTAILSDG
                     SKSLIYIHAAFAAFDIYLLKRHETEQGYIDDGSFSAEEIQDARKYWETYLKNDPQRQK
                     ELSDMITAGLPGAQAGFTLDNFREAVAKYAGMDERKLQTHIAYFLDRVVPVANAAGVR
                     LCCHSDDPAFTPFLGTPRTVGGCKGYKFLLDHGCGANLCIGSLMANTKNRDITRMIRE
                     IADYGRLKGMSIDEIFPHIHLRPVETDGKNFREGHHAEHREELAKVVHTLVDLGWQGV
                     FRPDHAPTSDHGFGRPGYDVIGRGYGANLILGLFEMAEITKAARTKAVEAVIKACNGD
                     LALREEAIKAAGEAEAEYIRKKIAFIKIPFAYSTQDICAVLK"
     gene            complement(20774..21589)
                     /locus_tag="PKI68_02150"
     CDS             complement(20774..21589)
                     /locus_tag="PKI68_02150"
                     /inference="COORDINATES: protein motif:HMM:NF006132.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX52527.1"
                     /translation="MKNLFELSDKVIAVTGAAGVLAGGTAKYLQEQGAHVIYLDLFQD
                     KVDAAVAEARKISDKCCGYACNVLDQTVLDTVYEKIMSDIGRVDVLINGAGGNMPGAT
                     IGPDQDVFDLKIEDYSKVLDLNLKGTVMPTMTFAKAFKAQKSGCVVNFSSMSATQALT
                     RVLGYSNAKAAIDNFTKWMATEMALKYGSKVRVVALAPGFFIGNQNRALLINADGSYT
                     ARGQQVINKTPFRRFGEQEEVYGTIHYLISDAAAFVTGVIVPVDGGFSAYTGV"
     gene            21710..22681
                     /locus_tag="PKI68_02155"
     CDS             21710..22681
                     /locus_tag="PKI68_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNX52528.1"
                     /translation="MAVGQKQIAEKLGVSIALVSRVLSGRAEEIGIAPDTIVRVLKTA
                     KEMGYVPSAAALSLKGKSTRTIGVVVYDFNDPFFGALIQQIQTQAHQHNYSLVLAGFL
                     NRSPDKQDLQSLHKHAMDGLIVLGSDTETEWLQDFDHLPVARIGHGSANEHSLKITVD
                     ENDAAKQLVQHLAARGRKNLICICGSLPAHRLRQTALEKAASAAGIRMTTTIVSAEKD
                     PFAIGRQVTQQLLNTAGDADALICNMDQIAMGALHVLYASGIKVPEQIAVTGFDDIPA
                     AAQFIPPITTVRQPIQEIVRLTFQAIIKPDGTQTVYLPGKLIARETT"
BASE COUNT         5127 a         6629 c         5898 g         5043 t
ORIGIN      
        1 ggcatatcac cgggcggcag aaaaacatcg tcaccgggat tgataaagaa ttcaaacgga
       61 accgaccgca gtgccagttc ggccatttcg tcccaggtgt aagtgaagcc ctgctccgcc
      121 caggtgcggc ggatttcctg catcatccag agtccggaga ggtttttcag gaaacggatg
      181 gtgccgcaca cacccacttc attcgtgaag ttggccgcca acacattgcc gttaataacc
      241 ggagccggaa gctctgttcc gagcagcgac caggtgccgg agctgaggaa tgcgcattgt
      301 tcgccttccg caaccggcac ggcggcgaag gcgctggcgg tatcgtggct gccgacatta
      361 acgaccggca gtccctgata atcgccaagc acagttccgg gatcagcaaa cggagcaaac
      421 aggtctgtac ggatattaag tttcgcaagc agatcaaagg cccagtcttt ggcgaccgga
      481 ttatacatct gagaggtgct ggcaaaggtc cggttggttg cgcgcctgcc agtcaaccag
      541 tagttaagca gatcgggagc aaacagcagc ttatccgcct gcaaatactg agtatcgttc
      601 gactgcgcca gtgcataaat ctgatagagc gagttcagct gcatgaactg gatgccggtc
      661 tggttgaaaa cggtttcttt gccaacggcg gcaaatacct tttcaggcat gccgtttgtg
      721 cgtgagtcgc ggtagttgac cgggttgccg agcagtttcc cctgagcatc aatcaacccg
      781 aagtccacgc cccaggtatc aatgccgatt gaaaccagcc cgccgccgaa ttctttcttc
      841 gccagcgcga taccttcctg gatattgcgg aaaagatgaa taaaatccca gtagagcgtg
      901 ccgttgattt ccgtcggtcc gttccagaac cggttcatct ctttgagcgt gagcagaccg
      961 ttttcaagcg taccgacgat tgtgcgtccg ctggaagcgc ccagatcaac tgccaaatag
     1021 tgcctgctca tgtttgctcc ggcaaacgtc agccgtaaac cggaccatag gtctggcagg
     1081 cgcggaagtc tgcgcctgcc aacccttcgg tgccgaatga actccatgag ctcgggcgat
     1141 agaccttttc gtcttcgacg ttatgcataa agaccggaat ccgcagcata gaagccatcg
     1201 tgatgaggtc ggcaccgatg tgtccgcagc tgaacgcacc gtggttggcg ccccacttcg
     1261 ccatcacttc atagacgtcg gtgaaggcac ctttgccggt gatacgcggc gcaaaccagg
     1321 tcgtcggcca ggtactgttg gtccggttat ccagcacatt gtggactttt tccggaagtt
     1381 caaccgtcca gccttctgcg atctgcagca ccgggccgag acctttaacg aggttgaccc
     1441 ggctcatggt aatcggcatg ccgccctgag tgacaaactt ggaagaccag ccgccgccgg
     1501 gaaaatattc cgtcaccgcc ggatgccatg tagtggcatc cagacaggct ttggcttctt
     1561 tgcggctgat ttcccagaac ggtttcatag caggcaggcc gtccctgctc tgccgtccgc
     1621 tgccatcgag cgcggcggaa ccggagttga tcagatgaat cagaccgccg gcagcggccc
     1681 cttcgacctc gtaaccggtt acacgcctga tcgcttcggg actccagtag gtgcggacat
     1741 cggcgaaaac ctgcgcggtg ctggtgagca ggtaggcgaa aagcatcggt acgccgttaa
     1801 gactgtcatt ctccgtagcg acgaggcgcg gcatgcgaat gccgtgccag tcgaacgagc
     1861 tgttggaaac ggcttcaaga aagtcgccgt ttggcgagca gtccgtccag ttgcgctggc
     1921 cctgaaatcc ggagaggatc gcgttgtggc cgcgcgcttc ttctttgtac cccatcttat
     1981 caagcttatc gctgccgacc atcaggtcac gggtgatgag cgccatctta acggagtctt
     2041 cccactccct gttgagctgc ttgcgggagc gtttggtcgc ggcaccgttc gggtcgcggc
     2101 cttcccggca gttttctttc acccaggcaa gcgctttttc gtactcagcc tgatcgtaaa
     2161 tgccatcccg catccggcgg gtaaattcag tcaggtcgat tgcttcaact cgcatgccga
     2221 gatattcttc gaaaaagttt tgatcgacaa tcgaaccggc aatgcccatc gagcaaccgc
     2281 ccatggaaag ataggattta cctttcatca ttgccacggc caggccggct ctggcgaaac
     2341 gcaggatttt ttcttccacg tcggcaggaa tggatgtatc gcccgcatcc tgtacatcac
     2401 ggccgtaaat tccaaaagcc ggcaatccct tctgcgtatg cccggccagc acggcggcga
     2461 gataaaccgc gccggggcgc tcggtgccgt tgaaccccca gaccgctttc ggacgcagcg
     2521 gatccatgtc catggtttca gaaccatagc accagcacgg cgtcaccgtc agcgagacgc
     2581 cgacgttttc acggttgaac ttttcagcac aggcggcggc ttcagccaca ccgccaatgc
     2641 aggtgtccgc gattacacat tccaccggct tgccgttcgg gtatttaagt gagctgctga
     2701 taagcttcgc ggcgctcctg gccatagtca tgacctggcc ttcaagcgat tcgcgtacac
     2761 cgccgtaacg cccatctatt gtgggacgga ttccaatttt cgggagcgcg gtaatccatg
     2821 ttttttcagc cataggaatc tcctttattt taaaatcccc ttttttatac acacatcccg
     2881 ccccggcttc ggtcgtcttt cggatggtat attttacaca ttccattagt ctcctgccca
     2941 tgaacgagat catcagttac ggcagattcc ggtgcaaaga actcgctccg cataaaaacc
     3001 ggggaatgga gatcacgtac attgaaaaag gagtcctgga gtggatggtg gaaggtgttc
     3061 cggaaaaaat tgaaccggga tcggttttct tcaccctgcc gtggcaggta cacggaagcc
     3121 tgcatccgtc cgaaccggat aatgtcgtct ggcatgttct tttccacctc gatgaggatt
     3181 atccggctcc gcgcaggcaa tttggattcc cgcccggctt cggattcagt ccgatcgaaa
     3241 tgaaaatgct cagcactgca tttacgtccg gttcaaaaca ctgttttcgt gcaacgccgg
     3301 ccatgcactg gctgatgccc gcactcatca acgaactgca aaccacccat gaactggcaa
     3361 aggcgcactc gatcagtctt ctgcgtgctg tgctggtcga gttgaagcgg attgtaagcg
     3421 gcgatgccgt cgatacagat acacacacct ggtccgagaa gaaagtacaa gccctgctga
     3481 ccgacctctc atcgagctgt gatcagcaat ggactctgaa acagatggca ggccggtgcg
     3541 gcattcgtcg aacccggctc aatacagttt tccagaaact gaccggcagt acaccgatgg
     3601 aatatctcac acgtttgcga atggaacggg cgaaaaccct tctgcgagaa accgggatca
     3661 agatcatcga catcgctttt gaatgcggct tcggcagcag tcaatatttc gccaacacct
     3721 tcaaacaagc caccggtatg acaccgaccg gctaccggat acactgcgcg agacttacca
     3781 ccgacgaatt gctcaagtgg aaaaatgtca agttccgcag tgaacaggaa gagcggcggc
     3841 gcgtacagaa tttcagcgca aattgagcaa tttgctgatg atccggatgc acggcggacg
     3901 gcggatcccg caccgattca agattacaaa gcatctgtat cagagcttaa gagaaccctg
     3961 cgggaaagcc gtcagccaga actggaccca gaactgcgtc ttgtcttctt catcaattcg
     4021 gaacccgacg cccatgccga tacagttaaa tttgtgatta acaagtatct tgcgttcgta
     4081 ccactcattg aatttttcat cgtaactggc cgaaaattca taggaccagt cgtcgttcgg
     4141 gaaaagttta acgcgcggtg ttataagcga acgggttccg gccagataac ggtattccat
     4201 gctgtactcg ctcttgtcgt cagagacaaa cttgagtcgc gcattggccg gatttacatc
     4261 atgggtgtac cagttgtatt caagatctga ctggacaaag aaattgtcgg tcagactcag
     4321 ttcggtatcg gccacaagat tgctgaagct cttttgaccc tcaggcgtct caaaacgtcc
     4381 ggtcgtgtaa atatcgctat cgacaaaatt ggatacccgt ttaagaccgc gcttcgtctg
     4441 cagaagattg cgggccccga agcgtacttc attctgtttg tcgagcgcgt caacggaatc
     4501 gaactggaaa aggttcgtcg gagtcatact gggttcaggt ctccagctgt agtcggcata
     4561 cggttcgact acatggcgca gtccatcgcc gaagtaactg ctcttttcgg taatcggctt
     4621 gtaagctttg aaggatgtca aggtgcccat ctctacgata ttacgatagt cggcattggc
     4681 cccttcggcc gtctcgctgt accatgtccc gcgataaccg gcgcgcggaa tcacgttaaa
     4741 gaattcctta aagcgcagcg gcaggaaaat gcggtcatag gtgtcaaacc gggcgctgcg
     4801 gtaattatca gcccgcggaa tcgggaacat attggtttcg gcattttgct tctggaggaa
     4861 ggcgacattg ttttcgcttt caaaataaaa cggcgagtca ccgagccgcg aacggtacca
     4921 gtcgtaggtc atttccggca gacggtcgac ggtcgtgtag aaatcattca tgcggcggtc
     4981 cacacgcagg ctggcggcgt aatcgtcggc ggaacgctga acaacggcgt aattttccgg
     5041 attggcttcg ttccggaagt cgtcattgaa aaagtccttg agaacaagcg gatcgctcag
     5101 ccagttgacc tttgtggcaa agtaagtctg ttcatcaatt ttttcacggt ctgtaatctt
     5161 cacgcggtac cgctgcgaat caatcagact cttatcaaat cccatattat ggcggctgtt
     5221 gctgctgccg ttgtacggat cgttgtcatt aatgtagtag gtctcaatac cgccaccgcc
     5281 ggtcggcgtg gtccatctga aatcctgacc gaggccgaga ccgcggcggg aataaagatc
     5341 gaaatgagat acagaccgga gccagtcggt cggatgaact tctgcgcggt acttgatgta
     5401 gccgccgacc cgtccgccat agccgatacc gcaggtgaat acgccgtgat ccagatgacg
     5461 ctgccaatag ggtgtgtaga aaatcggaac agggccggcg aagaaggtca cgcccttggc
     5521 cttaacgaaa accccggttt ccttatcgtt aataatccgc agctctttcg ctttggcata
     5581 gatcgccgga tgttcaccgg agcaggtggt catcatgaca ttatgcatga gaaactcatt
     5641 ggtcgaaacg cgctcagtgg tttccgcagt aatgtaggcc ggctcaaaat acatggccga
     5701 tttaccgaag gtaccggcct gggttttgta attataagcc agctcctgac cttcccagta
     5761 attgccgtcg cgaaccatcc ggatgtttcc actggctttg atctcgccgg tttctgaatt
     5821 taccgtcacg tgatccgcct gaaggctggt accggcctgc tgaacagcaa cgttcccgtc
     5881 gccaatgaga agcttgccgg aatattccat ccggtcggca gttacttcaa ccgcattagg
     5941 atcatcggcc gtgcgttcag gaagaggctt tgcttcgaca cgcacactgc ccgacccgct
     6001 gccggcggac gcagcagccg cttcgccgcc accggctgca gcggctccgc caccgccgga
     6061 ttcaccggat gtacctgcca ccgggacaat cggcgctttc gtgccgtcgg ccagcaaagc
     6121 ttcgccgtga gggaagagaa cgcgcagacg ttcggccgat tttgcggcgt attcggtgtc
     6181 gccgaattta cgaatcacct tgttgtaata aatcgcggcg gaattcgtct gcggcggacg
     6241 gggaatgcgt tcgtaaaaac ggccgacctc atagttatgt tccgctgaaa gcgtatgcag
     6301 ttttacgctg aagtcatgta cttccgcgat gtgctgtgag ttggtataaa gctcaggaaa
     6361 cagtccggcc gagagttcgg cctgttcgcg gatgtcgatg ctgtacggag tggaatcaac
     6421 cagtgttttg aagctgtcga ttttcgcaac agcggctttc tcagcgaatg gactgttcgg
     6481 ataacggtat tcggttgttg cgtaggcgac aacagccagc tccggatcgt tgtttttctg
     6541 gtaggcctgt ccgatcatgt actgcatctc aggggcacgt tcccattgcg gagcgttctt
     6601 gatgatcgac tcgaacagcg gcacggcacg ctcaggggcg cggtagccgc cgaagagcca
     6661 gcgcatccgt ttgcggttca tctctttttc ggcaagggtg tactgctgat caagaatgga
     6721 attataattt gtgatgctgg tgtagtactg ctgaatcagt tcttcgtagg cggtaaaggc
     6781 ttttttgttt ttgccctttt ctgcataaat ttcagccacc gtctttttgg cggttgccgc
     6841 gtgagcatct tccggccagc gcttcacaaa aatctcaaac tgcttccgcg cgctccggat
     6901 gctgcctttt tctttcagcg actcggcata ctgccagtgc tcttcagccg tcgccggttt
     6961 ataggcgaac ggattcagat aaaaatagtt accgctgcgg gtttcctgat acggcatggc
     7021 cgttgaacag ccggtcacgg caagcagcgt gaaaacggcc agaaagcccg cgataaaagc
     7081 gcagttctgt tttaaattca tatgcgccag agagtacgga agcccctccg ccttggcaag
     7141 agcgaagccg aggtttccgg agtcctggat taaaactcaa cccgcggaac cacagacgct
     7201 tccggcgccg actcaaacgg cgtgcggcgg gaaaatccga acatgccggc ccagaccgat
     7261 ccggggaacc ggcggatcga cgtattatag gcctgcacca tttcgttata gcggcggcgt
     7321 tccactgaaa tacggttttc cgttccggac agctcgtcct gcagcgccat aaaattctga
     7381 ttggctttca gctccggata gttttccgcc accagcagca ggcggctgag cgcgccctgc
     7441 atctctccgg cagccttaat cttttcgtcc ggcgtggagg ccaggcccca ctgactgcgc
     7501 agacgggtga cttccgtcag aagtcctttc tcgtgtgcgg catagccttt caccgtgctg
     7561 atcagattcg ggattaggtc aaaacggcgc tgaagaacgg tttcgacgtt ggcccactgg
     7621 gccgtcaccg cttcgtcctg ggaaacaaaa cggtttttcg atccgattgc tgatccgccg
     7681 atcatcacaa ccaccagcaa aataattccg agtacaatcc ataccttctt catcgtcatc
     7741 ctccatttct aaattcggtc aaccgcatcc accaccgctt caatagaagc cagatactct
     7801 tcgaagagcc gcccggcgtc tacatttttt attttcagcg taccggcttt cagcgcctga
     7861 atccgccgaa agctttcgag cttcaccgga acatgcccgg tgagtttttc caacgcctga
     7921 aacttttctg ccggcaccgg attttcatag agacgcagcg cggcgcggaa cagcgtcagc
     7981 acgccggaaa gcgaaccggt caacaacgcc gtcagccggc gcggatttcc gctgacggaa
     8041 agataactgc ggcaaagctg aatcaccgcg gagcgcagtt cgcgctcgac ctgcagccgc
     8101 agattcgcca tgccgggctc aatcgcggcg atgacatctt cgccggacag aacgcggcgg
     8161 gcatcgcgca tatcgagcag ttcaaccgga aacacatcgg cggcatccgc cagccgcttg
     8221 cgcgtaaaca tcaatggcgg cggattcccg gcccgctccc aaaccgcaac cggtttcgcc
     8281 agattccgca gaaccgccgg cgaaagatca ttcagtacaa cgagcagatt atagtccgat
     8341 cctttcgcgg cgtaatcgcc cgccgccgca gaaccgtaaa gcaccacggc ctgcagatta
     8401 tctccgcaaa cggttttcag tttttcagtg aactgttcag gactcatctt tttcataaaa
     8461 cactccctgc tctaccagcc gcgcgacgct ccgccgcccc ccgacatacc gccaccgaaa
     8521 ccgccaaagc ctccggagct tcctccgccg aaaccgccgc cgctgcgtcc gccgccaccg
     8581 cccataaaca aaagcaaaag ccacggatga cgaatcacag cgaagattat aattataaaa
     8641 aagatcagct gaataagtcc gccacctttt cgatcctgcg attgcgaacc gctatagcgt
     8701 tcctgattcc gaactccggt cagttcaacg ccggcggcgg aagccgctac cgaagccagc
     8761 gccacagcgc cgcttcgcaa cccgccgctg taatcgccgt tgcggaatgc gggccgcacg
     8821 tactggtcaa tcagccgccc cgccgccgca tccggcagaa cgccttcaaa accgtatccg
     8881 gtttcgatac gcactttccg gtcttcaatc gcggcaatga gtagaagtcc gttgtctttg
     8941 cctttttttc cgattcccca gcgtgcaaaa agccggttgg caaaatcgtc gatctgtccg
     9001 ccttcgagag atttcagtgt aacaaccgcc acctgcgcgc cggttttctg ttccagttcg
     9061 gcgagagtac tctccacagc ggcgcggtcg gaagaattca tcacattggc gaaatcgctg
     9121 acgtaaccgg ccggtttaag cgaattcaac aggcgatccg tatccgcccg gaccggcaga
     9181 aatccggcca gtaaaacaag aactgtgatg aaaaatctgc gcatgtccgg aaccgtaccg
     9241 gcttttatcc tgttttgaaa tatttcattc actatttata acccttgaaa ataaaggtca
     9301 aaaccgcaat cccgctaatc cttatgcttt tcatcagatt ttaatttgac agtcccgtcg
     9361 gaaagcgtat cttgcgcttc atgcattaag agaggcgtga ggtaaacgcc cggcaacctg
     9421 ataacataag gtgccacggc cttgcaaaag gcgatgcgcc ccgcaagggg aaagttgacg
     9481 gtcaaccgca tctcacctcc gccctctcta actgactaca aaagtaaaga gagggacaga
     9541 aatgagtaag aacactgaaa cgctggcgct tcatgccggc tacaaaccgg atcccacggg
     9601 cagctgtgcg gttcctgtct acagaacaag tgcctatgtc ttccgggaca cggagcacgc
     9661 cgccaacctg ttcggcttga aagagctggg caacatttat acgcggctga tgaacccgac
     9721 gaacgacgtg cttgaacagc gcgtcgctgc gctcgaaggc ggagccggcg ctctggcact
     9781 ggcatccggc acggcggcga tcttctacag catcattaac ctggcacagg caggcgatga
     9841 aattgtgtcg tccagcaacc tctacggcgg aacctatacg cagttcaata acatcctgcc
     9901 gcagctgggc atcaaggtga aatttgtcga tccgtccaat ccgcagaact tcgccaaggc
     9961 catcacggaa aaaacgaaag ctctctacac cgagaccatc ggcaatccgt cgctcgacgt
    10021 ggcggacatc gaagccatcg cgaaaatcgc gcatgctcac ggcctgccgc tgatcgtaga
    10081 cagcaccttc accacgccgc acctgctgcg ccccattgat cacggcgcag acatcgtggt
    10141 gcactcgctc accaagtggc tgggcggaca cggcaacggt atcggcggta ttgtcgttga
    10201 ttccggaaag ttcaactggg cgacgggtaa gcatcccctg ctctccgagc cggatgaaag
    10261 ttatcacggc atccgctacg cgcatgacct gccgccgtca ctggcgccgc tcgcctacat
    10321 cctgcggatg aggctggtgc cgctgcgcaa cctcggtgcg tgtctttcgc ccgacaacgc
    10381 gtggcagttc ctgcaaggca tcgaaaccct cgccctgcgc atggagcgtc acggcgaaaa
    10441 cgcgctggcg gttgcgaagt tccttgagaa acaggctgaa gtcgaatggg tccgctaccc
    10501 cgggctcgaa agtcatccgt cgcacaagat cgcgaagaaa ctgcttaaaa acggtttcgg
    10561 tgcaacggtg gtcttcgaag tcaaaggcgg cgaagcagcc ggcaagaagt tcattgaaaa
    10621 gcttcagatc ttctcgcacc tcgccaacgt cggcgacgcg aagagtctgg cgattcatcc
    10681 ggcaaccaca acgcactcgc agcttaccgc cgaacagcag gcggaaggcg ggattacacc
    10741 cggactcatc cgcctgtcgg tcggccttga gcacatcgac gatatcctcg ccgacctcaa
    10801 acaagcgttg aaataacgag gaagcgaaat attggaataa tggaagtttg ggttcgcccg
    10861 acttctgtta ttcctttatt tctttattca tgaaaaccgt tgaaacacag tttttcacct
    10921 atgatgcact gacactgcaa agcggtgtcc ggttcggccc cgtgacgctg gcgtacgaaa
    10981 cctacggcac gctcaacgaa aagcgagata acgccattct gctgtttcac gcgctgaccg
    11041 gctcgcagca tgccgcaggg gtcaaccctg ccgtgccagg catcggcgaa cggtggaatg
    11101 cagaatgcca gaacggctgg tggagcgatt atctcggtcc ggctaaagtc gttgatacca
    11161 accgcttctt tgtcatctgc atcaattatt tcggcggctg ttacggttct accggcccgt
    11221 cttcgatcaa tccggcaacc ggaaaaccgt acggcggaaa atttccgcgc atccatttca
    11281 gcgacatcgt cgactcccag cttcctctgc tgaaacatct ccgtattgaa aagctgcacg
    11341 ctgtcatcgg cgcgtcactc ggcggaatgc tgctggccaa cctggcggcg cgctatccgc
    11401 acattacaga ccgcctgatc gccatcgcct gcgggatcga gccgaccacg ctgatccgcg
    11461 cccacaacat tgagcagatt ctggcgattg aaaacgaccc gcacttctgt aacggcgatt
    11521 actatgacgg cgaaccgccg gtacgcggac tcgctctggc gcggatgatc tcgcataaaa
    11581 cctttgtttc gctcgatttc atcgaagagc gcgccaaaaa ccgctgggag caggaatacg
    11641 acgagttttc gtggtataaa gtccggactc cgcttgagtc gtacatcctg catcaggggc
    11701 gcaaactggt ccggcgtttt gacgccaaca cctacctgca gctgatcgcc gcatggtcgg
    11761 actatcatct gctgcgcgac accggagcac cggactatgt tactttgttt aagaactgcc
    11821 gccatcagcg ttatacgctc ttcaccattg atgatgacta ctgtttctat cctgaagagc
    11881 aggaatacta ccggaagaaa ctcgaagagg gccgcgttcc ggtcaaacac atcacggtcc
    11941 actccaacaa aggccacgac tctttcctgc tggaaccgca cctttacgag caggcgatcc
    12001 gggaagcgct gaacagttga caccctcatg cacatgagcc agcacatctt atgggcggcc
    12061 ggatggatgt tccacctgac tgcctttgca ctcgtcacct ttcactgcct tcaacgccgc
    12121 cgcaacgcca gctccaccct tctctggata ttcatcacct ggtctatccc ggtcctcgga
    12181 gctctcttct atctcagctt cggcattgac cgcgtcgatg accgcggcta tctcaaattc
    12241 ctcaccgatg agcatctgct gaaaacccga aaaggaaccc gcgacgcggc tccccgcgct
    12301 tactggcatc acctcaccgg ggcgcctgcc gaaaacgaat ttcagcggga attcaaccgg
    12361 acgctcgacg ctctcattcc cgaccatccc gcgctcgccg ggaatcggct tgttccgctg
    12421 gtgaacggcg atgaagccta tccgcgcatg ctggaagcca ttcgacaggc cagacaccat
    12481 attcaccttc agagctatat catcggcaac gacagcgtca gccgcgaatt catggcggcg
    12541 ctttccgcca aagccgccga aggggttcaa gtgcgcctgc tttatgaccg gttcggttct
    12601 acttatgcct tgttcggcgg actgttccgc aaggcacgta aaacacccgg cttcaaggtc
    12661 gccggctgga cacaggccaa ccttcttaaa cgccagtttc agatcaacct gcgcaaccat
    12721 cgaaaaacgc tggtcgttga cggagaaaaa gggtttttcg gcggaatcaa cctgctggac
    12781 gaccacatca gccgtccggg caaaccgccg atccgcgatt atcacttcga agctgaaggt
    12841 ccgattgtgc aggagctgca atactctttc ctgcgcgact ggtatttcat caccgaggaa
    12901 tccccgggcg aactgctgac cgaagaatat tttccgcaga ccaaaccggc cggaaacatc
    12961 acggcccggc tcatcagcag cggaccctct acggttgaag acgtcgccat tgaaagtttt
    13021 ttcaacagca tcgttcttgc tcaaaagcag attctcgctg tcactccgta ctttgttccg
    13081 ccggcggata ttctgcgcgc gctgcgttcc gccgcgttgc ggggagtgga tgtacaactg
    13141 gtcgttccca aagaaaacaa tcaccgctac gccggttacg ccagtcgcgc actctacgaa
    13201 gaacttctgc tggccggtgt acggatattc gaacgccgcc cgcccttcat gcacgctaaa
    13261 gcattgatca tagacgggga attcgctctc gtcggcacag ccaatctgga tgtgcgcagc
    13321 ctgagcctga actacgaaac ctgtctggcc gtttacagcg ccggctttgt cgacgtcatg
    13381 aaacgcatca tccatgaaga catcgcctgc agcgatgaag tcctgctggc cgactggcaa
    13441 agccgtccga cgcgttatcg cctgctcgaa aacctcgcca gcctcatgac tccgatcctc
    13501 taaatataaa cagtccgcta tatgcggacc ctgcgccggg cagtcccggc ctgccggaca
    13561 tccggctcag caaaccttcg attcatcctc ataccggagc gggatcttat aattcaaggc
    13621 cggacggaaa actcctgaaa atgctcccgt acggatgagg tgcgcggaaa aaagctcttt
    13681 cttcgacagc tggttaatcc ggacttcccg caaaacagga acggcgaata ctggaaatac
    13741 ccaaggaatt caaccgtcct gcaggtcgat accgaaatag ttcttcgcgt tgttgaagca
    13801 gatgttttcc accatcgtgc caagcagatc catgtccgcc ggaatttctc cgttctcgac
    13861 atcattgcca atcaggttgc aaaggatacg gcggaaatat tcgtgacgcg gatacgaaag
    13921 aaaactgcgc gaatccgtca gcataccgat gaatcggctc agcagtccga gcgaggaaag
    13981 cgcgttcatc tgtttttcca tgccgtcctt ctgatcaaga aaccaccagc cggagccgaa
    14041 ctgcattttg ccggggaaac tgccgtcctg gtagttgccg atcatcgtcg ccatcagttc
    14101 gttatcgcac gggttaatgt tgtagaggat tgtcttcgcc agctgattcg agctgtccag
    14161 ccggtcgagc agtctgatca ggcccggccc ctgacggtaa tcggcaattg aatcgaatcc
    14221 tgtatccggc ccgattttcg aaaacaggcg gctgttatta ttgcggaaca ccccgacatg
    14281 gaactgctgc gtccagttgc gtgagtgatt catctttccg acttcataca ggaagaccgc
    14341 ttcaaatttt tcacgctcgt ccagcgtgac gcggtcgccg ttcatcgcct tctgaaaaat
    14401 gatattaagc tccatcgaag aggcctgcgc atccggcaca taggccactc catgatcgga
    14461 gagacggcat ccggccgcat gaaaaaattc atgccgtttc tccaccgccg cgaccagtgc
    14521 ggcgaaatcc ttaatctcca cgccggaaac tttttccagt gtctggatat attttttcca
    14581 aaccgtgaca tccgacagat tcatcgcctt atccgggcgg aacgtcggca gtactttaat
    14641 ttcaaatcgg tcagccgcaa tctgcttgtg atattccagt gaatcgaccg gatcgtcggt
    14701 ggtgcaaacc agctttacgt tcattttgcg catcagatta cgcgcggaaa attccggcgt
    14761 gcgcagctgc gcggtggcag tctcaaaaat ctgctccgct gtttccgggc tcaacagcgt
    14821 gtcgattccg aagtagcgct gaagttccag gtgcgtccag tgatagagcg gattgcggat
    14881 cgtctgcgga acggtctcgg cccacttccc gaatttgaca cgccacggcg cactgccggt
    14941 gacgaactct tcggtgacgc cgttggtgcg catggcgcgc catttgtagt ggtcaccgcc
    15001 cagccagatt tctccgagat tatcatagcg cctgtcttcc gcgatttccc tcggagataa
    15061 atggcagtga tagtcgtaaa tcggcatttt cgcagcgtgg ctgtgataga gcacttccgc
    15121 tgtttttgtc tgcagtaaaa aatcacgatc cataaaagat ttcataggtc ctctccgaat
    15181 ttttctttgt acgcccagag accgagcgcg ggattactga tataaaaaat ttcttcgccg
    15241 tccggcatcc tgttgaagcc gtctttgagt tccgccggat tatagtgcgc cgccatctct
    15301 gcatacggca ctgcgttgaa gccgaccgaa cggacttcat cgagtgtgat atccgggccg
    15361 gggcaatacg tgatgctgaa acggccttcc gatgagccgt gaatcaggtg cgccgccgcg
    15421 ctgaggttgc tgcgcagttc ctcgttctct ttgaccgctt tgagcgtggc cggcgttccg
    15481 cgataaccgt acttgcggat cagctcatca atcttcggat cttcgccgaa ctccttgagc
    15541 gccggagcca gcacgatcag ctcgccgcca tcggcgatcg ccatgcgcgt acggtaaacg
    15601 gacttattac cgagccacgt actctggaac tcgtgaggat ccagatagac caccaccttc
    15661 ttcggctcgc gatccagcag cgtgatgttt accttgcggg ccagcttgca ggcctcgtta
    15721 taaacctgca catcatcccc ggcgtagaat ccgcgcaggt gcattttgcc ggtatcatag
    15781 tcctgctgca ccaccgtcag aatataggtg atcgggagat caccgagaaa cgtgtctacg
    15841 gcataattaa acaggcggcg caccggcgta tcggtctgcc ctagaatcgt ctccatgttg
    15901 cagacggcac cgagaaagtg cgacttgttg atgatatccg aaccgcccgc gccgacgacg
    15961 atgtttttgg tgtagttggc catgccgacc acttcatgcg gcaccacctg cccgaccgaa
    16021 agaatcaggt cgtagccgtc gaagagcatc ctgttcactt cgacaccgat actgtaattc
    16081 actttcccgc cggaaagctc cgcgagcatt tcaccggaca cttcgccttt gcggacaatg
    16141 ccgttgcgcc agtcgtgcac tttgaacgcc ttcagcggaa tcgtctcgcc atacatcatg
    16201 cggagttgtt tctccgtcat cgcattatgc gttccgagag tcggcaggat atccacctga
    16261 acaccgtccg ccgtgagcag ttcatacgct atcgccgtga tcggccccgc ctgagagttc
    16321 aggcgggtgt gatccggagg cagaatcagt actttgcgca aaggacccgg gcggttttcc
    16381 aatatttggg aaaccagcgc acgcaactca tcgcacgaga tggacatctc ttcgccaaac
    16441 cgactgatca tcgcggactc cttttagaca atgtaggtcg aggccgccgt atcgccgccg
    16501 cggccggtcc agttggtgtg aaagaattcg ccgcgcggtt tatcaatgcg ctcataggtg
    16561 tgcgcgccga agtagtcgcg ctgcgcctgg agcagattgg ccggaaggcg tgcagcgcgg
    16621 tagccgtcgt aatacgacag cgcggcgctg atggcaggca tcgggatgcc gagttcaacc
    16681 gacttgcaga tcacgcggcg ccagccggcc tgtgcgtttt caaccgcctt ggtaaagaac
    16741 ggggccagca gcaggttgac cagatccgga ttcgtgtcga acgcttcctt gatcttgccg
    16801 aggaacaccg agcggataat gcagcccccg cgccacatca gcgcgatacc gccgttgttg
    16861 agcttccatc cgaattcgtc ggccgccgca cgcatgagct ggtagccctg cgcgtatgag
    16921 acgatcttgg aggcatagag cgcctgcttg agatcttcga tcagcgcggc tttgtcgccg
    16981 gtaaattttg cgccggggcc tttcagcgtt ttcgaagccg caacacgctc atccttcagc
    17041 gcggagaggc agcgggcaaa gaccgcttcg ccgatcagcg tcagcggctg gccgagatcc
    17101 agcgcggcaa cggccgtcca tttcccggta cctttctggc cggcagtgtc gaggatctga
    17161 tcaatcaccg cctcgcctgt tttggttttg tagccgagaa tgtcgcgtgt gatttcgatc
    17221 agataggaat cgagctctcc tttgttccac tctgtgaaca cctcgtgcat ctcgtcgttc
    17281 gagaggccga gtccttcctt catcatctgg taggtttcgc agatcatctg catatcgccg
    17341 tactcgatac cgttgtgaac catcttcacg aaatggcccg cgccgttttc gccgacccat
    17401 tcacagcagg gttcgcccgc agcggtttgt gcagaaattt tctgaaagat cggtttcacg
    17461 gccggccacg ccgcaggcga gccgccgggc atgatcgacg ggccgagcag cgcgccttct
    17521 tcgccgccgg aaacgccggt tccgataaag agcaggcctt tgctttcgac atacgccgtg
    17581 cgccggatgg tgtccgggaa atgcgaattg ccgccgtcaa tgatgatgtc gcccggctcc
    17641 aggtgaggaa tgacctgttc aatgaaatca tccacagctt ttccggcttt gacgagcatc
    17701 atcactttgc gcggacgttc gaggctggcg cagaattctt tgatgctgtg acagccgatg
    17761 aaatttttac cggctccgcg tccggcggta aatgcatcaa ctttctcgac cgttcggttg
    17821 aataccgcca cggtgaagcc tttgctttcc atattgagta caaggttttc acccattacc
    17881 gccaaaccga tcagtccgat atctgctttt ttcttcattt tatatccttt cattaaacat
    17941 gatgcatcgc gaacctaccg cctaacccgc gcattgccgt tccatatact ccaaagctgt
    18001 tcttcgtcag caggctgagc ataatctgtc agcatcgttg cggcaagtgc tccgcttgcc
    18061 cagccgaacc gggcacactt cttgacatcc cagcctttca gtattccgta aagcagcccg
    18121 ccgacaaagc cgtcaccgcc gccaatccgg tcaataacgc cgatttcgcg cggtttgatg
    18181 atttcccatt ctttaccgtc cgtcaccagc gcaccccaca tatggctgtt tgctgaaaca
    18241 acctcccgaa gcgtcgtccc gaaccaggtc gcattgggat aggcggcctg aacccgtccg
    18301 atcatttcct tgaagccgtc aattttatcg tcgagtcctt tgcctccggc ctcgggacct
    18361 tttatgccga ggcagagctg aaaatcctct tcgttgccga tcaggatgtc gctgcgtgaa
    18421 gcaatttccg caaatgcggc ggacagttct ttttcccggc ctttccagaa actggcccgg
    18481 taattcagat cgaacgaaat ctgagagccg ttctctttgg caatgcgggc gatatccaga
    18541 cagaacttac ttgttttagg cgacagagct gcgatcagcc cggaaagatg aacgattccc
    18601 gctccttctt tggcaaaaat ccggtccaga tcaaaatcgt cagcacaaag ttcccgtccg
    18661 acttcgccgg cccggtcatt atgaactctc ggcccgcgcg cgccaaagcc gctgtccgcc
    18721 atattgatct gatggcgata gccccacggt ccgccctgat cgaactccgg cccctcaaag
    18781 tccattcggc ggcttctaag gttatctttt atgaagtgag aaacagggct gcccttgata
    18841 aacgctgtca aaactttgac gggcattcct agataggaag cgatgctggc tacattgctt
    18901 tccgcgctgg tcgcctgcat tttgaaggta tcgctgcaat gaaacggctg cgaatcaacc
    18961 ggggtcagcc ggatccccat gctggtagca atgaccagag aatatttttt tgacgatttt
    19021 tcagtcattt ttctccatca atggaattca caatccgccc tttgaactcc gatataagcc
    19081 gagagactgc cgccggacaa atcgatgccg gtgatcataa tatttctcaa ttgtttgaga
    19141 caatgttttt tgaataattt tatcaagcaa aaatattgtc ttttcggaca gccttcgcca
    19201 tattgcgctc cctttaccca aaaacaggag ccttcccgtg agtgaaattt ataataaaaa
    19261 ctggaaccgt gagcgcgtcc gcggcggcat gcgctggttc ggccccgaag acattgtcac
    19321 catccggaac atccggcaaa cccccggaat caccaatgtg atcaccgctt tgcaccatat
    19381 cccgccggga caggtctgga cggatgcgga gatagccaag cgcaaaatcg aggtggaatt
    19441 ctggccgcag tccgatgccc ccgccgacct ggaaggcatg cctctgttcc ggtggtatca
    19501 ggtaaacgga aatccgaccg gtctggtctg ggatacagcc gaaagcctga ttttcacaga
    19561 agacatcaag aacggcagcc cgaaccgcga ccggcacatt gaaaactatc aggaaagcct
    19621 gcgtaacctt ggacgcttcg gcgtgtgcaa tgtcatcggc aactttatgc tggttgccga
    19681 ctggacccgc accggcacgg caattctgtc cgacggctcg aaaagcctca tctatattca
    19741 cgccgccttt gcggccttcg acatctatct gctcaaacgc catgaaaccg aacagggtta
    19801 tatcgacgac ggatctttct ccgccgaaga aatacaggat gcccggaagt actgggaaac
    19861 atacctgaaa aacgatccgc agaggcagaa ggaactctcc gatatgatca ccgccggact
    19921 gcccggcgca caggccggat tcactctgga taatttccgc gaagcggtcg ccaaatacgc
    19981 cggcatggat gaacgcaaac tccagacaca catcgcgtat tttctcgatc gcgtcgtccc
    20041 cgtcgccaac gccgccggcg tccggctctg ctgtcattcc gacgacccgg cttttacgcc
    20101 gttcctcgga acaccgcgca ccgtcggcgg ctgcaaaggc tataaattcc tgctcgacca
    20161 cggctgcggc gccaatctct gcatcggctc tctgatggcc aataccaaaa accgcgacat
    20221 caccagaatg atccgcgaaa tcgccgacta cggacgttta aaaggaatga gcattgatga
    20281 aattttcccg cacattcacc tgcgcccggt tgaaaccgat ggaaaaaatt tccgcgaagg
    20341 ccaccatgcc gagcatcgcg aagagctggc caaagtcgtc cacaccctgg ttgacctcgg
    20401 ctggcaaggc gtcttccgtc ccgaccacgc accgacatcc gaccatggat tcggccggcc
    20461 cggctacgat gtcatcggcc gcggctacgg ggccaacctg atcctcggcc tgtttgaaat
    20521 ggccgagatc accaaagccg cgcgcaccaa agcggtcgaa gcggtgatta aggcctgcaa
    20581 cggcgacctg gctctgcggg aagaagcgat taaggcggcc ggcgaggccg aagccgagta
    20641 catccggaaa aagatcgcct tcatcaaaat cccgtttgcg tacagcacac aggatatctg
    20701 tgcggttctg aaataaggaa tccttcacgg tctggaacac cggctctgcc gggttccagg
    20761 ccttgaaaaa tatttaaacg ccggtgtaag cggagaaccc gccatcgacc ggaacaatga
    20821 cgccggtgac aaatgccgcc gcgtcggaaa tcagatagtg aatggtgccg tacacctctt
    20881 cctgttcccc gaaccggcgg aaaggtgttt tattgatcac ctgctgcccg cgagccgtat
    20941 agctgccatc tgcattgatc agcagcgccc ggttctggtt gccgatgaaa aagccgggag
    21001 ccagcgcaac cacacggact ttacttccgt atttcagcgc catttcggtc gccatccatt
    21061 tggtgaagtt gtcgatagcg gctttggcgt tggaatagcc gagaacacgg gtcagcgcct
    21121 gagtggcgga catggatgaa aagttgacca cacagccgga cttctgcgcc ttgaaagcct
    21181 tggcaaaagt catcgtcggc ataacggttc ccttgagatt caaatccagc actttactgt
    21241 agtcttcgat tttcagatcg aacacgtcct gatccgggcc gatggtggct ccgggcatat
    21301 tgccgccggc gccgttaatc aggacatcaa cccgtccgat atcggacatg attttttcat
    21361 agaccgtatc cagcaccgtc tggtcgagca cattgcaggc atagccgcag cacttatcag
    21421 aaatcttccg ggcctcagca acggccgcat ccactttgtc ctgaaacaga tccagataaa
    21481 tgacatgcgc cccctgctcc tgcagatact tcgccgtgcc gcccgccaat actccggccg
    21541 cgccggttac cgcaatcacc ttatccgaca gttcaaaaag attcttcatt aaaattctcc
    21601 agtggtttag ctcaattgtt tgagatagta gttatatgcc tgcttcgcaa gcaaaaactt
    21661 gctttttatt tcccatccat tcactgtctc aatagtttga ggaaatcaaa tggctgtcgg
    21721 tcagaaacaa attgcggaaa agctgggcgt gtccattgcg ctggtgtcgc gcgtcctgtc
    21781 cgggcgggcg gaagaaatcg gcattgcacc ggataccatc gtacgggttc tgaaaaccgc
    21841 aaaagaaatg ggctatgtcc ccagcgcagc cgctttgtcg ctcaaaggaa aatccacccg
    21901 taccattggt gtggttgtgt atgattttaa cgatcccttc ttcggagccc tcatccagca
    21961 gattcagact caggcgcacc aacacaacta ctctctggtg ctggccggct ttctcaaccg
    22021 gagtccggac aaacaggatc tgcagtctct gcacaaacac gcgatggacg gactgattgt
    22081 actcggatcg gatactgaaa ccgaatggct gcaggatttc gaccatcttc ccgtcgcccg
    22141 gatcggacac ggatcagcca atgagcacag cctgaagatc acagtagatg aaaacgatgc
    22201 cgcaaagcaa cttgtgcagc atctggccgc ccgcggacgg aaaaatctga tctgtatttg
    22261 cggatcgctg ccggcgcacc gtttacggca aacggctctg gaaaaagccg cgtctgccgc
    22321 cggcatccgg atgacaacaa ctatcgtatc cgctgaaaaa gatccttttg ccatcggccg
    22381 gcaggttact caacagcttc tgaacaccgc cggcgatgcg gacgccctga tctgcaatat
    22441 ggatcagatc gccatgggcg cattacatgt gttgtatgcc tcaggcatta aggttcccga
    22501 acagattgcg gtcaccggat tcgatgatat tccggctgct gcgcaattta ttccgccaat
    22561 caccaccgtc cggcagccga tccaagagat cgtccggctc acctttcagg ccatcattaa
    22621 accggacggt acgcaaaccg tttatctccc cggaaaactt atcgcccggg aaacaacata
    22681 aaagcggcag aactctg
//
LOCUS       DAOPZO010000015        21597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_2480_length_21597_cov_9.117491, whole genome shotgun sequence.
ACCESSION   DAOPZO010000015 DAOPZO010000000
VERSION     DAOPZO010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 21597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21597
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2480_length_21597_cov_9.117491"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..75)
                     /locus_tag="PKI68_02160"
     tRNA            complement(1..75)
                     /locus_tag="PKI68_02160"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Gly,seq:ccc)
     gene            244..328
                     /locus_tag="PKI68_02165"
     tRNA            244..328
                     /locus_tag="PKI68_02165"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:278..280,aa:Tyr,seq:gta)
     gene            complement(486..3866)
                     /locus_tag="PKI68_02170"
     CDS             complement(486..3866)
                     /locus_tag="PKI68_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNX52529.1"
                     /translation="MAKLVLDPESPNPRLIHLDKPSTLIGTASDADVSIKSELLSNHH
                     ARIEHKPDDGYYIISLANTPGVFVNGSPITFQRLKHGDKIEIGDVKAVLLLDSADESA
                     HDVPGHGSPVQEMMLPASMMSAGLTTIPRGPARCPQCGMPLMPGLPSCPHCGMPFSNL
                     PAMPMNFIPPLPASQIGPGILPLIAFLAALTVVGAPIALVLGLMTLSIIRRRGGTARD
                     HALAKWAIGLGLVWLMAGAVAAGGLLRQAQKRKQLEVVAVHEVKVIHALKNLACAEKY
                     AQTIECFDADADGQGEYGTLSVLAQTKSPFFDTGLANGEAYGYRFTIRGAAESQFLAV
                     AEPVNYGKTGIRTFAIDQTGQIRGGDTEGKPFGQGASVLPVLQGERNAYYEIDDEIAK
                     DVLNYVKTLSSTLADQEKKQRILKRLHDEYALTSVGRELGGMEASVDRFVSEQQAQTI
                     YLDAKAALAKGSQDVALAKLTEIKEKHPSFSQIAAVERELGDLRSVIAQKREQEAQAL
                     LIQAEALERQGQRPQDVQDMYQRIAKLYPDTDVAARVSSLKPELQRQLRERDAEKIFS
                     DLMELSPERDFDEILNRANQLRRNYSDTDLLSKAKTELAEKERKARANSWRAKTEQNM
                     AAGRMRGALGQLESAARENPDLLYDLRDLCIQLYRSVADSLVKEGDERKALEYYEKLS
                     RLLQASGSKDQVSPDLLAKLHIDVGQADFDHKDYKEARWHLASAAWKYPDDAQFNMRL
                     GAASLYTGLYRPAEESLTRALSVQPNLVSARLYRAYLNMRVELSSERVLADRFKQEAA
                     PAETAKAVKDESASGKPQQTSSSSISISVADESGSGDGVVVNTDVVDGDSGIAAPKSL
                     DVKSSNVGASWFSRFAENGQTAKSPVPEPTDIAPFLNFNYQSSSSILPGIMQVLDDLQ
                     RQRAEKDLAASTGRAKAAAMSQQSARDDMRQMGKMSLRVSEQKNIVAYHTQLRQLRAL
                     HLKDADARKELCDMMNEMKQRINSARSDIQTAGRQQSRIQSLTESVLSQIDKKYRLFD
                     KAETLISSCTEDESQTRKRMLELAEEMLMKYNSNNASDVTSFDKQIADFRSKLLDEDG
                     VLEIDRALRALRDSMDVRVDLDAILRAAEGNAPVAAGE"
     gene            complement(3962..11008)
                     /locus_tag="PKI68_02175"
     CDS             complement(3962..11008)
                     /locus_tag="PKI68_02175"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HNX52530.1"
                     /translation="MNLNADQTLIARIIEQTARHPDAAAIRCGKVSVSYAELNRRSLE
                     LAQVLRIHGAGRDVPVALCMDRSPEVIIALLAILRAGSAWLPLDPVYPRDRRIFMVAD
                     AAAPIILGKSNDDRTLSPKAVFIALDQPTPKAGPVRIELPSADNLAYITYTSGSTGKP
                     KGVLCPHRGALNMLDDARRRQPLGPGDTCSWWASLSFDVSVYEIFSPLLCGATLVIVP
                     DGIRLDAAKLTDWLCKEKITSAFLPPSMIADLEDWTREHPGQSCLRRLLTGVEPVPER
                     RLMNIARAVPGLCIINGYGPTEAAVYCTCYCVDGSSGEVHRNTPIGKPLDGVEIYLFN
                     EKGGPVADGQEGEIYIGGVQVARGYLNCPRLTAEAFLDTPHGRLYRSGDRAVRLPDGN
                     LMFIGRVDDQIKFLGYRVEPGEIESALCRVEGVREAVVVSREDISGIHRLVGYYTVQA
                     GRTPAVDRIRQSLERDLPQYMIPSLFIRLTHIPLTAAGKIDRKALPPPAPGDLAQLRA
                     AEFIEPVSATEKTVAGLFSAILKTGPSGLNDHFFMLGGHSLLATQLASKIQTELQTDV
                     PLKDIYGNPTVGKLAALVDLLRSAGTVSSEIPPAAGRTSYPLTPSQRTMWMLHQSDRS
                     GILSNIPEVIYLNGPLNVDVMEQAFNEVIRRHDSLRMVYSMEKGEMVQRPLAKVYVPV
                     KLTDLSGLDAAERDRRAAEMRRTNGLHVFDLSTGPLIHAELVKLAPNRFQLFFNIHHI
                     AADGWGISRFTTEFAELYEAFLDRRPSPLPPPGLQYSDVAVWIDERIRSGALQSQLDY
                     WKDRLAAPRPDLHFRLDHSRPDVSSHRGARHAFIVSPELTGALTALGRRANASLFMVL
                     AAIWQTLLHRATGSTDVITGTAIANRNHPQLENVIGTFINALALRTDFSGTPEFSDVL
                     DNVRRTALEAYANQDIPFEKVMEVVGDGTRYPVFRNSLILHNMPLPTKKFAGLTLTDD
                     EIGNDTSKMDMLLYFIEREGQLEGQLEYDAELFDAATAEQTAADFLMLARQIVKDPHR
                     PLGDYLQAGEEEAPTCFVIGEGSLCLRCTEVLRKRGLRVLGLISPDAANRRWAKEKGI
                     PWHHPSEGFAEILSAQPFDFLFSIVNSCILKPDVLALPRRYAINYHDAPLPRYAGVYS
                     TAWAIINRERFHGVSWHVMADEVDAGDLLKQRTVDVFENDTSFTLNARCYDTAIEALS
                     ELALELVERREVRTAQDLRQRTYYPLHKRPAYGGLIDWSRPLAETDALRRALDFGNQP
                     NDLGLPKVLLGDDLYVLKSDGDMLTLDGDPVELRFSPVGEAADWIVNEPAFSNLGKKL
                     DALNAELARHERFWVRRFENFQPLEIPAGIDRSIHRYTLTDLPLFLCFLARFCSQEQF
                     TAGWKTETAVPEFFADTVPLYVELDAGASLADNMSAAEKALGICRKHRTYARDLFQRF
                     PSLHKKPVYSVVLSENEVSCPAKVWEHFRAFRHNAFLSRPLFSQSVLTVRDRNELEKF
                     QACTPGFGEARCIHQLFEAQAEKTPRATAVEGIDGKLTYAELNARADALAVQLQELGV
                     KPDTPVGLFVNRSLEMCIGILGILKAGGAYVPLDPEYPADRLEFFMNDTAMPLIVTVA
                     ALTGRISSAERKTICIDRIKAGDTKPSACIANSNLAYIFYTSGSTGQPKGVMVEHRNV
                     VNHCLASIQTYGISDKDRVLQFFSMNFDGSVEELFPAWASGGTVVLRSGELSSSVSEF
                     EKFIAARKITVVDLPTAYWHEWVRNLRTVSDKLRAVIIGGEKVSAELCRVWLQKGGSA
                     IRLFNTYGPTECTVVSTVHELTGAVTGEVPIGLPIAGTALYVADTRQQLVPVGMPGEL
                     LIGGEGVARGYFNRYDLTCEKFIRNPWGEGMLYRTGDSVCRQSDGRISFMGRVDNQVK
                     IRGFRIEPDGIAATLEQHSGIAQAVVAARDDLSEQKELSAYYIAEPGRSPSVSELRDF
                     LSSKLPEYMIPVAFMELDEFPVAPGGKVDRKALPSPVKNVIRSRENYVEPATPQQKTL
                     AEIWSAVLGMDAIGIHDNFFDLGGHSLLAIQLVERILSSGLSLTVAQLFQYPTIAQMA
                     EVASLRGDSEYKSLVCLKEGAPGRVPFFLLHSAPGDLLGYSNLVHNLPSDQPVYGFQS
                     LGLIDPEKVHSTIPEMAAYYVSVLREFLPEGPYLLGGWCYGGYVAMEMARQLMEQGCK
                     VNLLALIDAWAYPPAERRLAFYWRRIQLMRIIGFRPWLKIIQERIKNIFRDDAADAVK
                     MLDGMQAKEGVLANREEVYRRNREAALRYNPRFYPGRAVLFRSDELASWFLPDMTMEW
                     AALMEDQDIYLVPGGHRDLLREPAVKVLAAHLNASIEKAVEG"
     gene            11077..11742
                     /locus_tag="PKI68_02180"
     CDS             11077..11742
                     /locus_tag="PKI68_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNX52531.1"
                     /translation="METFKNSITVWNIHLPDHHGDTDLCRGLLAGEELERAAKFRKAA
                     DAEGFVLGRGLLRRILADCLNTTPSALRFNRNPHGKPFLEGGELEFNVSHSRDRLLIA
                     VTAGRTVGVDIEFLRDGLNMAAIAKRWFAPAEQEFFKTIANPADGFFELWAKKEAYVK
                     ALGVGIYKDLNSFAVPVGEKPFSPNIEPSGKWFFQTLKIDSGYAAAVVSEAPPVPVVL
                     RNF"
     gene            11746..12840
                     /locus_tag="PKI68_02185"
     CDS             11746..12840
                     /locus_tag="PKI68_02185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX52532.1"
                     /translation="MKISIGILAWNEASSIRSTIHSVFSQSLISNLRETGHRVEIICV
                     PNGCTDNTALAAQGAMLAAAEKLNYQENLRWRVHELKQPGKTNAWNVFIHELSDPQAD
                     VIFMVDADIQIYMPDTLSNMLTKLMQNPQAFICTDLPVKHIVFKQHYSLLDQISMHTA
                     RINQSASGQLCGQLYCARAAWLRRLWIPEGIIVEDGFIKKMAVSNFLTEPEDAARRLV
                     IAETASHVFEAYTRLPDVLATHRRQIAGYVIHRWIWEYLQEHQSEAPDAAALIENLNR
                     TAPGWSRRLIADKIKTRDYIRIYRILIATRFIRFRGFSSPKKAAYAPLLAIHILLDSI
                     QFLSALNMLCRGKLQKVWRDTRTTSGEINR"
     gene            12837..14003
                     /locus_tag="PKI68_02190"
     CDS             12837..14003
                     /locus_tag="PKI68_02190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX52533.1"
                     /translation="MKLAFAHHLDLQKKARARKPGAVGIHSAAENIALHLKEACEEFR
                     YLAPLHNLCSPAAAARILLCQKLTREKYYGWAEPSLSRGYGRQLARKLKDCPADAVLC
                     TEVKHAAFLKTGRPVVVWTDSLYGGLFDYYQTFTGLCRRTQRDLQEMDRAAVKNCARL
                     IFASDWAAKRAMELYGASPEQVKVVPYGANLTSGLSRTDAGKLVAEKSFDGVCRLLFT
                     GVEWKRKGGDTAIKAVEILNAQGIRTEITFLGTDPARHMKNLPEFVKSAGFLSPAKPD
                     ERARMETLYREAHFLIQPCRAECFGHIFPESSSFALPVVASNTGGIPTAVHEGINGYC
                     FDPEATGRYAECIGRLFSNPDEYRTLAMKAFDDYNARLSWSQAAGQVIKIIEVL"
     gene            14000..15151
                     /locus_tag="PKI68_02195"
     CDS             14000..15151
                     /locus_tag="PKI68_02195"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNX52534.1"
                     /translation="MNGKRITEIDGLRGFLALWVFVCHSILYANYPTKLPGPLEFWRA
                     AWHAVDLFVIITGFSMWFLLDHTKERYFTYLGKRFFRLYPVYIVLFLIGIPVALLEFK
                     GNELLGQPVANLQSWFSNLWKHIGLHAVMLHGVVPKTAAPPEATFAFLPPAWFVSYVW
                     QFYLVAPLLFLFNRSHGRFIFYGLLCAATVWFRNNLPQVPVGAFLPFHIEYLFYGALS
                     YKIVKACSEREIKIPVPLTAGAVVLSLLLFPQYKQLFLPTPIFLAKSDWWLPVSLWLI
                     FFSMLLDVHFGKLDIVSNALSRWINSKPMQFFGRISYSFYLVHALVIVSVRTLVAMKY
                     PELSKAVWLTVLVVLSFPASLGLSVVLHRWVEIPGAKLGKRMMGSTGSL"
     gene            complement(15146..15820)
                     /locus_tag="PKI68_02200"
     CDS             complement(15146..15820)
                     /locus_tag="PKI68_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52535.1"
                     /translation="MKVRELLRLLFWPLIILCFLVGAQTFFAHYTQGMSRLLDYFNGI
                     IDMREERTVGTWFETLLFALTGISFFLVSRHPALPKFGKTLLILMALGFCFLSADEML
                     SLHEFMGYEFEQATGIVKDTRLDQRGYSWVMLYAPAALIVCGLVSRSYYRMTKTSWNR
                     SASICYLLAWLAIELVVLCEMIDGWSIIERVDLSLAPCFEESLELVFIMLFYSANLLI
                     AEDAEL"
     gene            complement(15906..17147)
                     /locus_tag="PKI68_02205"
     CDS             complement(15906..17147)
                     /locus_tag="PKI68_02205"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNX52536.1"
                     /translation="MKKLILFSFLLLTALTGTAQTAVQTAQSDNSWRKAVSQFTVVRT
                     GTGYMNEADFTEFLDRAEGMHKRPGIFKMFTARPVKFYERWGMWPTLALILFGGLALN
                     LTPCVLPMIPVNLTIIGAGAQSESRRKGFALGAVYGLGITVVYGILGLAVVLGGAKFG
                     ALNANPWFNLGIAAIFLALALAMFNVFHIDLTKLHRHLNRRQIEEEFLAAFSIGGLSA
                     LLAGACVAPVVIAVLLLSGNLYTNGVHAGLALPFVLGLGMALPWPFAGAGLSFLPKPG
                     RWMEWVKRGFGVLILVLAIYYGMLGGRLLIARLAPPGGEEHLVFCNGQNTCFHDMLNE
                     AFAAGQPVLIDFWADWCKNCHAMDSTTFEAPDIKNRLTPYKVLKFDATNYKESPAKEI
                     LDHFGVMGLPTYIILKPVKGE"
     gene            17172..19217
                     /locus_tag="PKI68_02210"
     CDS             17172..19217
                     /locus_tag="PKI68_02210"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNX52537.1"
                     /translation="MNRPEHTALNILKQIRKADFTAFFAGGSVRDKLLGRTAKDYDIA
                     TSATPDQIEALFPKTVAVGKAFGVIIVVQDGTETEVATFRADGGYQDGRRPDTVEFCG
                     AEEDAKRRDFTVNGMFYDPLDDRIIDYVGGQEDLEKKIIRAIGDPDRRFAEDHLRILR
                     AVRFAHTLGFAIEPATRAAIRRHAPDLVKISAERIENEFSRTLTESLRPGDALRELVD
                     LGLMEFIIPEILPMIGQEQPPEFHPEGDVFEHTVLMLNLMNQQTEMPASQIVEQAFQP
                     AHPFRPVEQTAALHTSRRRLPHWEQDSRIYFITFRLADSIAQEKLNQWQEELEIWKKQ
                     QPDPASEETERERARLYHERQEKWLDKGYGSCILKNPEISAIVEQTLRHFDGERYRLG
                     DYVIMPNHVHLIIEPAAGQPLSGIMQSWKGYSAREINKVTGSKGALWMDESFDHIIRS
                     EESLEKFTDYMRQNPVQAHLASTGFRAGTGSLSDIRQAGKPASHGEPPFPPVHSSNFK
                     KLLAYTALLHDVGKPATAFMGADRLRFHGHERKSAEMAEEILRRLKLPVKEIRRIVTA
                     IDGHMRFKDVQKMNKSTLRKLMGAETFELELELHRIDCAGSHGLLDNYDFLLKKADEM
                     KNEPILPERWITGKDLREIGIASGPHMGELLQLAYDAQLEGRFPDRETLLAWLKTQL"
     gene            19294..19842
                     /gene="folE"
                     /locus_tag="PKI68_02215"
     CDS             19294..19842
                     /gene="folE"
                     /locus_tag="PKI68_02215"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNX52538.1"
                     /translation="MEELIRKLLVELGENPEREGLKSTPARVAESYKFLTHGYHLNID
                     DVLNDAFFQAEDSHMVIVKDIEVYSLCEHHMLPFIGKCHIGYIPDKRVVGVSKLARLV
                     DVFARRLQIQERLTDQIAHTIMEKTGAQGVGVVIEAQHLCMMMRGVEKQNSKMITSAM
                     HGTFRSQLATRTEFLRLIGTIG"
     gene            19988..>21597
                     /gene="recN"
                     /locus_tag="PKI68_02220"
     CDS             19988..>21597
                     /gene="recN"
                     /locus_tag="PKI68_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNX52539.1"
                     /translation="MLSRLNIRNLALVDNITVSFQSGFTVITGETGAGKSLLIGALRL
                     LLGERADKSIIRTGETSCSIEAVFELRDPSAVNSALEEVGLAPCDEGQLVVRRTITES
                     SSKNIVNDSPVTLPVLKALGDVLVDMHGPYDHQSLLKTDAQMDILDAFGQLAADRAAY
                     QEKYKRFQALKNRLNELSNLDDSGLAEQIDLLAWRVKEIEEAKISEEDETAVQQEHEQ
                     IANAQNILELAQTAVGGLTEAEYSAIEGLSTARKACAQLAKYLPDAETWAEELEGAAR
                     TAQEISAAIQSAVEEVDAGPERLQFLDERLALYRNLKRKIAPAVPEILAKCDEWKEKL
                     KELQSRDEQRAAVSTALDAALIETEQAGRILRKKRQKVAGDLADAITAELRDLGFEHG
                     LFEVQLLDADEPCASGLDEIEFGFAPNAGESMRPLRAIASSGEISRVMLAVKAVLARH
                     DRIPLLVFDEIDANVGGEIANAVGDKLAQVGRTHQVIVITHLPQVAVCGQTHFSVSKK
                     VQDGRTFTQIAALTGGTRAEEIARMLGG"
BASE COUNT         4844 a         6450 c         5892 g         4411 t
ORIGIN      
        1 tggagcggga gacgggagtc gaacccgcga cgtcaagctt gggaagcttg cattctacca
       61 ctgaattact cccgccacga aaagaccggg attctctgac gggctctgct aactttcaag
      121 cgtgtttttc caatgtttgc aaaaataacc ggcaccccgt gccgattctg ccggaaaatt
      181 tcaaatcggg gtggacatcg gatttttttt ggctatggta tccgttctct caaccgagaa
      241 aacggtaggg taccgaagcg gtcaaacggg gcagactgta aatctgctgg ctatgccttc
      301 gaaggttcga atccttcccc taccaccatt ctttaatttt ccttccagaa cctgcccttt
      361 ccctaaggaa aaagcgtttt ggacacacag ctgctccgtc aacggaagcg gaaaaatcca
      421 caacgcgaag tgaagtcccc ggcatggttc tcgcgggctc aatttatccg gccataattg
      481 caggtctatt ctcctgcggc aaccggcgca ttcccctctg ccgcgcggag gatggcatca
      541 agatccactc gcacatccat cgagtcccgg agagcccgca aagcccggtc aatctctaag
      601 acgccgtctt catcaaggag tttgcttctg aaatcagcga tctgtttatc aaaagaagta
      661 acgtcggaag cattgttgga attatacttc atcaacattt cttcggccag ctccagcatc
      721 cgcttgcgcg tttggctttc atcctccgta cagctggaaa tcaacgtctc ggctttatcg
      781 aaaagcctgt atttcttatc gatctgtgac aggacgcttt cagtcagcga ctgaatgcgc
      841 gattgctgcc ggcctgccgt ctgtatgtcg gaccgggcgc tattgatacg ttgtttcatt
      901 tcattcatca tgtcgcacaa ttcttttcga gcgtcggcat ccttgaggtg gagcgcgcgc
      961 agctgtctca gctgagtgtg atacgcaaca atatttttct gttcgctcac tcggagcgac
     1021 atcttgccca tctggcgcat atcgtctctt gctgactgtt gcgacatggc agccgccttg
     1081 gctctccctg tgcttgctgc cagatctttt tcagctctct gccgctgcag gtcatctaaa
     1141 acctgcatga tgccgggcaa aatgctgctg ctgctttgat aattgaagtt cagaaacgga
     1201 gcgatgtctg tcggttccgg cacaggcgac ttcgcagtct gcccattttc cgcgaaccgg
     1261 gaaaaccagc tggccccgac gtttgaggat ttcacatcca gactttttgg cgccgcaata
     1321 ccggaatctc catcgacgac gtcggtatta acaacgactc catcgcccga tccgctttcg
     1381 tcggcaacgg agatactgat actgctgctg ctcgtttgct ggggtttgcc tgatgcggat
     1441 tcatccttca cggcctttgc ggtttccgcc ggagccgcct cctgtttgaa acggtccgcc
     1501 aggacacgct cggacgagag ttcaacccgc atattgagat aggcgcggta caaccgggcc
     1561 gataccagat tcggctgcac gctcagcgcc cgggtcagcg attcctccgc cggccgatag
     1621 agccctgtat acaaacttgc tgcaccaagt ctcatattaa actgcgcgtc gtccggatac
     1681 ttccatgctg cgctggccaa atgccaccgg gcttctttgt aatccttgtg atcaaaatcg
     1741 gcctgcccga catcgatatg cagcttcgcc agcagatcgg gactcacttg atccttcgag
     1801 cccgacgctt gaagcagccg gctcaacttc tcgtaatatt ccagcgcctt gcgttcatcg
     1861 ccttctttca ccagagagtc cgcaacactg cggtacagct ggatgcacag atcccgcaga
     1921 tcatacagca gatccggatt ttcgcgcgcc gctgattcta attgtcccag cgccccgcgc
     1981 atgcggccgg ccgccatatt ctgctcggtc ttggcccgcc atgaattcgc gcgggccttg
     2041 cgctctttct cggccagctc cgttttcgct ttgctcagca gatccgtgtc gctgtaattg
     2101 cgccgcaact gattggcccg gttcagaatt tcgtcaaagt cccgctccgg cgagagttcc
     2161 atcagatcgg aaaatatttt ttccgcatcg cgctcgcgca actgccgctg cagttcaggt
     2221 ttcagagacg aaacgcgggc agccacgtcg gtgtcggggt agagtttcgc aatccgctga
     2281 tacatgtcct gcacatcttg cggacgctgc ccttggcgct cgagcgcctc cgcctggata
     2341 agcagggcct gagcctcctg ctcccgtttt tgtgcgatca ctgaacgcaa gtcgcccagc
     2401 tcccgttcca cggcggcaat ctgcgaaaag gagggatgtt tttctttaat ctccgtcagc
     2461 ttggcgagcg ctacatcctg gctgcccttc gccagagcgg ccttggcgtc cagatagatg
     2521 gtttgcgcct gctgttccga cacaaaccgg tctaccgacg cttccatacc gcccaattcg
     2581 cgcccgactg atgtcagcgc atattcgtcg tgcaagcgct tcagaatccg ctgtttcttt
     2641 tcctgatcag ccagcgttga ggagagagtt ttgacgtaat tcagcacatc tttggcaatt
     2701 tcgtcatcaa tctcatagta ggcgttccgc tctccctgca ataccggaag caccgatgca
     2761 ccctgcccga aaggttttcc ttcggtatcc ccgccgcgga tctgtccggt ttgatcaatt
     2821 gcaaacgtcc ggatgccggt tttgccatag ttgacaggtt cggctacggc caagaactga
     2881 ctttccgccg cgccgcggat cgtaaaccgg tatccgtacg cttctccgtt agccagaccc
     2941 gtgtcgaaga acggggactt ggtttgcgcg agaacagaca gcgtgccgta ttcgccctgc
     3001 ccgtccgcat cggcatcaaa acactcgatg gtctgggcgt acttttctgc gcacgccaga
     3061 ttcttgagag catgaatcac tttgacttcg tgaaccgcca caacttcgag ctgtttgcgc
     3121 ttttgggcct gccgcaaaag cccgccggct gccacggctc ccgccataag ccataccaga
     3181 cccagtccga tggcccattt tgccaaagca tgatctctcg cggttcctcc gcgacggcgg
     3241 ataatcgaca gcgtcatcag acccagcacc agggcaatcg gtgcgccgac cactgtcagt
     3301 gccgccagaa atgcgatcag cggcagaatg cccggcccga tttggcttgc cggaagaggc
     3361 ggaatgaaat tcatcggcat ggccgggaga ttggagaacg gcatgccgca atgaggacag
     3421 gacggcagac ccggcatcag cggcatgccg cattgcgggc atcgggccgg tcctcttggt
     3481 atggtggtca gcccggcgga catcatcgaa gcaggcagca tcatctcctg taccggcgat
     3541 ccatgacccg ggacgtcgtg cgccgactca tccgcgctgt cgagcagcag cacggctttc
     3601 acgtcgccga tttcaatctt gtcgccgtgc ttcagccgct ggaacgtaat cgggcttccg
     3661 ttgacaaaaa cgccgggcgt atttgccaga ctgatgatgt agtatccgtc atccggcttg
     3721 tgttcaatcc gcgcatggtg attcgacagc aactcgctct tgatggatac atccgcatcg
     3781 gatgccgttc ctatgagtgt tgacggctta tctaaatgaa taagacgcgg attcggcgat
     3841 tcaggatcca atacaagctt tgccatacgg tcagcctttc cttctcatcg gaattcgata
     3901 aacacgcgac atcttagcct gctttccccg tcttgcatca aaatacggcc gaaaaactta
     3961 atcagccttc aactgctttt tcgatactgg cgttaaggtg cgcggcaagc accttaacgg
     4021 ccggttcacg cagaagatcc cggtgtccgc cgggaaccag ataaatatcc tggtcttcca
     4081 tgagtgcagc ccattccatc gtcatatcgg gcaggaacca ggaagccagt tcgtcggaac
     4141 ggaaaagcac tgcgcggccc ggataaaacc gcggattgta ccgcagcgca gcttcgcggt
     4201 tgcgccggta aacttcttcg cggttggcca gcacaccttc tttcgcctgc ataccgtcaa
     4261 gcattttgac cgcatcggcg gcatcgtcac gaaatatgtt tttgatgcgt tcctgaatga
     4321 ttttcaacca cggccggaaa ccgatgatgc gcatcagctg aatccgccgc cagtaaaagg
     4381 ccagccgccg ttcggccggc ggataggccc acgcatcaat cagcgcaagc aggtttacct
     4441 tgcagccctg ctccatcagc tgacgcgcca tttccatggc aacataaccg ccatagcacc
     4501 agccgccgag cagataaggt ccctccggca gaaactcgcg cagtacggaa acgtaatagg
     4561 ccgccatttc cggaatcgtg gaatggactt tttccggatc aatcagacca agcgactgaa
     4621 aaccgtacac cggctggtca gacggcagat tatgcaccag attcgaatac cccagcaggt
     4681 cgccgggcgc actgtgcagc aggaagaacg gtacgcggcc cggagcacct tccttgagac
     4741 agaccagcga tttgtattcg gaatcgccgc gcagactcgc cacctcggcc atctgcgcga
     4801 tggttggata ctggaaaagc tgcgcgaccg tcagagacag tccggacgag agaatccgtt
     4861 caaccagctg aatcgccagc agcgaatggc cgccgagatc aaaaaagtta tcatggattc
     4921 cgatggcatc catgccgagc accgccgacc agatttccgc cagcgttttc tgctgcggcg
     4981 tcgcaggctc aacatagttt tcacgcgacc ggatcacatt cttaacgggc gacggcagcg
     5041 ccttgcggtc tacttttccg cccggcgcaa caggaaactc gtccagctcc ataaaggcga
     5101 ccgggatcat gtattccggc agtttggagg aaagaaaatc ccgcagttcg ctgacagacg
     5161 gtgaccggcc cggttccgca atataatagg ccgacagctc tttctgttcc gaaagatcat
     5221 cgcgtgccgc caccaccgcc tgcgcaatgc cgctgtgctg ttccagggtc gccgcgattc
     5281 cgtccggctc aatccggaag ccgcggattt tgacctggtt gtcgacacgg cccataaaac
     5341 tgatgcgtcc gtcggactga cggcagaccg agtcgccggt gcggtagagc attccctcgc
     5401 cccacggatt acgaataaat ttttcgcaag tcagatcata ccggttgaaa tagccgcggg
     5461 cgacgccttc gccgccgatc agcagttcgc caggcatacc gacaggcaca agctgctgcc
     5521 gcgtatctgc cacataaagc gccgttccgg caatcggcag gccgatcgga acttcgccgg
     5581 tgactgcgcc ggtcagctcg tgaacggtgg aaaccacggt gcattcggtc gggccgtagg
     5641 tattgaaaag ccggatcgca ctgccgcctt tctgcagcca gacacggcag agttctgcgg
     5701 aaaccttttc accgccgatg ataacagccc gcagcttgtc cgacaccgtg cgcagattac
     5761 gtacccattc gtgccagtag gcggtcggca ggtcaacaac ggtgattttc cgggcagcaa
     5821 taaatttctc aaattccgaa actgaactgg aaagttcacc gctgcgtaaa accaccgttc
     5881 cgccggatgc ccaggccgga aacagttctt ccaccgagcc gtcgaagttc atggagaaaa
     5941 actgaagaac gcgatcttta tcgctgatac catacgtctg gatcgaagcc agacaatggt
     6001 tcaccacgtt acggtgttca accatcacac ccttcggttg tcctgtagaa cccgatgtgt
     6061 aaaaaatgta cgccagattc gagtttgcaa tacaggccga cggcttggtg tcaccggcct
     6121 taatccggtc gatgcaaatg gttttccgct cggcagagct gatacggccg gtaagcgccg
     6181 caaccgtgac aatcagcggc atggcggtat cgttcatgaa aaattccagc cggtccgccg
     6241 gatactccgg gtcaagcggc acatacgcgc cgcccgcttt gagaatgccg agaatgccga
     6301 tacacatctc caaagaacgg tttacaaaaa gtccgaccgg cgtatcgggc ttaacaccga
     6361 gttcctgcag ctgcacggcc agcgcatcgg cgcgtgcgtt gagttcggca taggtcagtt
     6421 taccatcgat tccttcgaca gccgttgcgc gcggcgtttt ttccgcctgc gcctcgaaga
     6481 gctgatgaat gcaacgggct tcaccgaatc cgggtgtgca cgcctggaat ttttccaatt
     6541 cgttccggtc gcgaaccgtc agaacagatt gcgaaaaaag cggccggctc aggaaggcat
     6601 tgtggcggaa agcgcggaaa tgttcccaca ctttggccgg acagctcact tcattttcgg
     6661 acaggacaac ggaatagacc ggctttttgt gcagtgacgg aaagcgctga aacaggtcgc
     6721 gcgcataagt tctgtgcttg cggcagattc cgagcgcctt ttcagcggcg gacatattgt
     6781 ccgccagtga tgcacctgca tccagttcga cgtaaagcgg aaccgtatcg gcgaaaaatt
     6841 ccggcacggc tgtctctgtt ttccagccgg cggtaaactg ttcctgcgaa cagaaacggg
     6901 cgagaaaaca gaggaacagc ggcaggtcgg tgagtgtgta gcggtgaatc gagcggtcaa
     6961 tgcctgccgg aatttccagc ggctggaaat tttcaaaccg ccgcacccag aaccgctcat
     7021 ggcgggccag ctcggcattc agtgcatcca gttttttccc gagatttgaa aacgccggct
     7081 cattgacaat ccagtccgcc gcttcaccga cgggagaaaa acgcagctcc accggatcgc
     7141 cgtccagcgt aagcatgtcg ccgtcggact tcagtacata cagatcatcg ccgagcagaa
     7201 ctttcggcag gccgagatcg ttgggctgat tgccgaaatc gagcgcgcgc cgcagcgcat
     7261 cggtttcggc cagcggacgg ctccagtcga tcaggccgcc gtaagcgggc cgtttatgga
     7321 gcggataata agtgcgttgc cgcaaatcct gcgccgtgcg gacttcgcgc cgttcgacca
     7381 gttccagagc cagttcgctg agcgcttcaa tggcggtgtc atagcagcgg gcgttcagcg
     7441 taaacgatgt atcgttttcg aatacatcca ccgtgcgctg tttgagcagg tcgccggcat
     7501 ccacctcatc cgccatcaca tgccagctga cgccgtggaa ccgctcccgg ttgataatcg
     7561 cccatgccgt ggaatagacc ccggcgtagc gcggcagcgg cgcgtcgtgg tagttgatgg
     7621 catagcggcg cggcaaggcc agcacatccg gtttgagaat gcagctgttg acaatactga
     7681 aaagaaaatc aaacggctgc gcggaaagaa tttcggcaaa cccttccgaa ggatggtgcc
     7741 atggtatgcc cttctccttc gcccagcggc ggttggcggc gtcgggcgaa atgagaccca
     7801 gcacccgcag gccgcgtttg cgcaggactt cggtgcagcg cagacagagc gaaccttcgc
     7861 cgataacaaa acaggtcggg gcttcttcct cgccagcctg caaatagtcg ccgagcggcc
     7921 ggtgtgggtc tttgacaatc tgccgcgcca gcatcaggaa gtccgctgcg gtctgctcgg
     7981 cagtcgccgc atcaaaaagt tcggcgtcgt attccagctg tccttcgagc tgcccttcgc
     8041 gctcaatgaa atagagcagc atatccatct tggatgtgtc gttgccgatt tcgtcgtcgg
     8101 tgagcgtcag gccggcgaac tttttggtcg gcagcggcat attatgcaga atgagcgagt
     8161 tgcggaaaac cggatacctc gttccgtcac cgacgacctc catgactttt tcaaacggta
     8221 tatcctgatt cgcatacgct tcgagcgccg tgcggcggac gttgtcgagc acgtcggaaa
     8281 attccggcgt gccggaaaaa tcggtgcgca atgccagcgc gttgataaag gtgccgataa
     8341 cgttttcgag ctgcgggtga ttgcggttgg cgatagcggt tccggttatg acatcggtcg
     8401 agccggttgc gcggtgcagc agagtctgcc agatggcggc aagcaccata aacagcgaag
     8461 catttgcgcg ccggcccagc gcagtaagcg cgccggtcag ctcgggagaa acaataaagg
     8521 cgtgccgcgc gccgcggtgc gaagaaacgt cagggcggct gtgatccagc cggaaatgca
     8581 gatcgggacg cggcgcggcc agcctgtctt tccagtaatc gagctgagac tgaagcgccc
     8641 cgcttctgat gcgttcgtca atccacaccg cgacgtcaga atactggagc ccgggcggcg
     8701 gcagcggtga aggccgtctg tccagaaacg cttcgtacag ctcggcaaac tcggtggtga
     8761 accggctgat gccccagccg tcggcagcga tgtgatggat attgaagaaa agctgaaacc
     8821 ggttcggagc cagtttgacc agttcggcat ggatgagcgg accggtggaa agatcgaaaa
     8881 catgcagacc gttcgtccgc cgcatttccg ccgcccggcg gtcgcgttcc gcggcatcca
     8941 gtccgctcag gtcggtgagt ttgacgggca cataaacctt agcgagcgga cgctgcacca
     9001 tttcgccttt ttccatggaa tagaccatgc gcagcgagtc gtggcggcgg atgacttcat
     9061 tgaaggcctg ctccatcacg tcaacattca gcggaccgtt caggtaaatc acttcaggaa
     9121 tgttggacag aatgccggaa cggtcggact gatgaagcat ccacatggtg cgctgcgacg
     9181 gagtcagcgg atacgaagtc cgcccggcgg ccggcggaat ttcgctggac accgttccgg
     9241 cgctgcgcag cagatcaacc agcgcggcca gttttccgac cgtcggattt ccgtaaatgt
     9301 ctttcagcgg aacatcggtc tgcagttcag tctggatttt tgacgccagc tgagtcgcca
     9361 gcagcgagtg accgcccagc atgaaaaagt gatcgttcaa tccggacggt ccggttttaa
     9421 ggatcgcgga aaacaatccc gcaaccgttt tttcggtggc tgaaaccggt tctataaatt
     9481 ccgctgcgcg caattgcgcc agatcgccgg gcgcgggcgg cggcagtgct ttacggtcga
     9541 tttttccggc ggctgtcagg ggaatatgtg tcagccggat aaacagcgac ggaatcatat
     9601 actgcggcag atcgcgttcg agtgactgcc gtattctatc aacggcgggc gtccgtcccg
     9661 cctgtacggt gtaatagccg acaagccggt gaataccgga aatgtcttcg cgggaaacaa
     9721 ccaccgcttc gcggacgcct tcaacccggc agagcgcgga ttcgatttcg cccggctcca
     9781 cacggtaacc gagaaactta atctgatcgt cgacccggcc gataaacatc aggttgccgt
     9841 cgggcagccg caccgcgcgg tcgccggagc gatagagccg gccatgcggc gtgtccagaa
     9901 aggcttcagc ggtcagccgc ggacagttga ggtaaccgcg cgcgacttgc acgccgccga
     9961 tataaatttc gccttcctgc ccgtcggcaa ccggcccgcc cttttcgtta aacaaatata
    10021 tttcaacccc gtcgagcggt ttgccgatcg gtgtattgcg gtgtacttca ccggaggaac
    10081 cgtccacgca gtaacaggtg cagtaaaccg ccgcctcggt cgggccgtag ccgttgatga
    10141 tgcacagccc cggcacagcg cgggcgatat tcatcagccg ccgttcagga accggctcga
    10201 cgccggtgag cagacggcgc aaacagctct gtccgggatg ctcgcgagtc cagtcctcca
    10261 gatcggcgat catcgaagga ggaagaaacg ccgaggttat cttttctttg caaagccagt
    10321 ccgtcagctt tgccgcatcc agccggattc cgtccggcac aataaccagc gtcgccccgc
    10381 aaaggagcgg tgaaaaaatt tcatagactg agacatcgaa actgagactg gcccaccagc
    10441 tgcaggtatc gccgggaccg agcggctggc gccgccgggc atcatcgagc atattcagtg
    10501 cgccgcgatg aggacagagc acgcctttcg gttttccggt ggagccggaa gtataggtga
    10561 tgtaggccag attgtccgca gacggcagtt cgatccggac cggtccggct ttcggcgtcg
    10621 gctgatccag cgcaataaaa accgctttcg gggaaagtgt ccggtcgtcg tttgatttac
    10681 cgaggatgat gggtgccgca gcatcggcaa ccatgaagat gcggcggtcg cgcggataaa
    10741 ccggatcgag aggaagccag gcgctgccgg cgcgcagaat cgccagcagc gcaataatca
    10801 cttccggcga acggtccatg cagagcgcaa ccggaacatc gcgtccggca ccgtgaatac
    10861 gaagaacctg agccagttca agcgagcggc ggttcagttc ggcataggaa acagaaactt
    10921 taccgcagcg gatagcagcg gcatcgggat gtctggcggt ctgttcaata atccgtgcaa
    10981 tcaatgtctg gtcggcattc aaattcataa gtatccggca ctcgcatcac ttgggtcagg
    11041 atggtatata aagcgccggt tttgatgaag aacaatatgg aaacttttaa aaactcgatc
    11101 acggtttgga acatccacct gcccgatcac cacggagaca ccgatctctg ccgcggcctg
    11161 ctggccggag aagaactgga gcgcgccgca aaattccgga aagccgccga tgcagaagga
    11221 ttcgttctgg gccggggact tctgcgcagg attctggcag actgcctgaa taccactcct
    11281 tccgcactgc gattcaaccg gaacccgcac ggcaaaccgt ttctggaagg cggcgagctg
    11341 gagttcaacg tatcccactc gcgcgaccgc ctgctgattg ccgttaccgc cggacgcacc
    11401 gttggcgtgg atattgagtt tctgcgcgac gggctgaaca tggccgccat cgcaaaacgc
    11461 tggttcgctc cggccgaaca ggagtttttt aaaaccatcg caaatccggc ggacggattt
    11521 tttgaactgt gggctaaaaa agaagcctat gtgaaggcgc tgggcgtcgg catctataaa
    11581 gacctcaaca gttttgcggt gccggtcgga gaaaagccgt tttctccgaa tattgaaccg
    11641 tccggaaaat ggtttttcca gacattgaaa attgattccg gttatgcggc ggctgtagtt
    11701 tctgaagcgc cgccggtacc ggtggtgctg cggaattttt aacccatgaa gataagcatc
    11761 ggcattctgg cgtggaacga agcaagcagc atccgttcaa ctatccacag tgtcttttcg
    11821 caaagcctca tcagcaactt gcgggaaacc ggacatcggg ttgaaatcat ctgtgtaccg
    11881 aacggctgca ccgataacac ggcactggcc gctcagggag ccatgctggc tgcggccgaa
    11941 aaactgaatt atcaggaaaa tctgcgctgg cgggtgcatg aactgaaaca gccgggaaag
    12001 accaatgcgt ggaatgtatt tattcacgaa ctgagcgatc cgcaagccga cgttattttt
    12061 atggtggatg cggacattca gatctacatg ccggacacgc tcagcaatat gctgaccaaa
    12121 ctgatgcaaa atccgcaggc atttatctgc accgatctgc cggttaaaca catcgttttt
    12181 aaacagcact acagtctgct cgaccagatt tcgatgcaca ccgcccggat caaccagtcg
    12241 gcttccggcc agctttgcgg ccagctctac tgcgcccgcg ccgcctggct gcgccggctc
    12301 tggattccgg aaggaattat cgtcgaagac ggctttatca aaaaaatggc ggtttcaaat
    12361 ttcctcaccg aacccgaaga tgccgcgcgg cggctggtga ttgccgaaac ggcctcgcac
    12421 gtttttgaag cctacacccg cctgcccgac gtactggcta cgcaccgccg tcagatcgca
    12481 ggctacgtca ttcatcgctg gatctgggaa tatctgcagg agcatcagag cgaagcgccg
    12541 gatgcggcgg cgttgattga aaatctcaac cgcaccgcgc ccggctggtc gcgccgcctg
    12601 attgccgata aaatcaaaac tcgcgattac atccgtattt accggattct gatcgccacc
    12661 cgcttcatcc gtttccgcgg attcagctcg ccgaaaaaag cggcttacgc gccgctgctg
    12721 gccattcaca tcctgctcga ctccatccag tttctgtcgg cgcttaacat gctctgccgc
    12781 ggaaaacttc agaaagtctg gcgcgatacg cgtaccacca gcggagagat caaccggtga
    12841 aactggcctt tgcccatcat ctggatttgc agaaaaaagc ccgggcgcgc aagcccggcg
    12901 cggtcggcat tcacagtgcg gcggaaaata tcgcactcca cctgaaagaa gcctgcgaag
    12961 aattccgtta tctcgcaccg ctgcacaacc tctgctcgcc ggccgcggcc gcacggattc
    13021 tgctctgtca gaaactgacg cgggaaaaat attacggctg ggccgaaccg tcgctgagcc
    13081 gcggctacgg caggcagctg gcgcgcaaac tgaaagactg tccggcagac gctgtactct
    13141 gcaccgaagt aaaacacgcc gcctttttga aaaccggccg gcccgtcgtc gtctggacag
    13201 actcgctcta cggcggactg tttgattact accagacttt caccggactc tgccggcgga
    13261 ctcagcgcga tctgcaagaa atggatcgcg cggccgtcaa aaactgcgca cgcctgattt
    13321 ttgcttccga ctgggccgcg aaacgcgcga tggagctcta cggcgcttcg ccggaacagg
    13381 tgaaagtggt gccgtacggc gccaacctga cctccggcct ctcgcggaca gacgccggaa
    13441 aactggtggc tgaaaaatcg ttcgacggcg tctgccgcct gctgtttacc ggcgtagagt
    13501 ggaagcgcaa aggcggcgat accgccatca aagcggtgga aattctgaac gcgcagggaa
    13561 tccgcaccga gattacgttt ctgggaaccg atccggccag gcacatgaag aaccttcctg
    13621 agtttgtcaa aagtgccggc ttcctttcgc cggccaaacc ggacgaacgc gcccggatgg
    13681 aaacactcta ccgcgaagcg cactttctga ttcaaccctg ccgcgcggaa tgtttcggcc
    13741 acatctttcc ggaatccagc agtttcgccc tgcccgtcgt cgcctccaac accggcggca
    13801 ttccgaccgc cgtgcatgaa ggcatcaacg gctactgctt tgatccggaa gcgacgggcc
    13861 gctacgccga atgcatcgga cggctcttca gcaatccgga cgaataccgc acgctggcaa
    13921 tgaaagcgtt cgacgattac aacgcgcggc tcagctggtc gcaggccgcc ggacaggtga
    13981 taaaaattat agaggtccta tgaacggaaa acggattacc gaaatcgacg gattgcgcgg
    14041 gtttctggcg ctctgggttt tcgtctgcca ctcgattctc tacgccaatt atccgacgaa
    14101 actgccgggt ccgctggagt tctggcgcgc ggcctggcat gcggtagacc tctttgtcat
    14161 catcaccgga ttttcgatgt ggttcctgct ggatcacacg aaggagcgct atttcaccta
    14221 tctgggcaaa cggtttttca ggctctaccc ggtttatatc gtactgtttc tgatcggcat
    14281 cccggtcgcc ctgctggaat tcaaaggcaa cgaactgctg gggcagccgg ttgcaaacct
    14341 gcaaagctgg ttcagcaatc tctggaagca catcggactg catgcggtga tgctccatgg
    14401 tgttgttccg aaaacggccg ctccgcccga agcgaccttt gcgtttctgc cgccggcctg
    14461 gtttgtctcg tatgtctggc agttctatct cgtggctccg ctgctgtttc tgttcaaccg
    14521 ttcgcacggc cgctttatct tctacggcct gttgtgcgcg gcgacagtct ggttcaggaa
    14581 caacctgcct caggttccgg tcggagcctt cctgccgttt catatcgagt atcttttcta
    14641 cggcgcgctg tcgtataaaa tcgtcaaagc ctgttcggag cgggaaatca aaataccggt
    14701 tccgctgact gcgggcgcgg tggtgctgtc gttgctgctg tttcctcagt acaaacaatt
    14761 attcctgccg acgccgatct ttctggcgaa atcggactgg tggctgccgg tcagcttatg
    14821 gctgatcttc ttttcgatgc tgctggacgt gcatttcggg aaattggaca tcgtgtccaa
    14881 cgcgttgagc cgctggatca attcaaagcc catgcagttt ttcggcagga tttcctacag
    14941 tttttatctg gtgcatgcgc tggtaatcgt atccgtgcgc acactggtgg cgatgaaata
    15001 cccggagctc tcaaaggcgg tctggctgac cgttcttgtc gtgttgagtt tcccggcatc
    15061 gctcggctta tccgtcgtct tgcaccgctg ggtggaaatc ccgggggcaa aactgggcaa
    15121 gagaatgatg ggatcgaccg ggtctttata actccgcatc ttccgcaatc agcagattgg
    15181 cgctgtagaa gagcataata aacaccagct ctaatgactc ttcaaaacag ggagccagac
    15241 tcagatcgac ccgttcgatg atgctccagc cgtcaatcat ctcgcacagc acaacgagtt
    15301 caatcgccag ccaggccaat agatagcaga tgctggctga ccggttccac gatgttttcg
    15361 tcatccggta atacgatctg gacaccaggc cgcaaacgat caaagccgcc ggagcgtaaa
    15421 gcatcaccca ggaataaccg cgctgatcca ggcgggtgtc tttgacgatt ccggtcgcct
    15481 gttcaaactc gtaccccatg aattcgtgca gggacagcat ctcgtcggct gaaagaaaac
    15541 agaaaccgag cgccatcagt atgagtaacg tttttccgaa cttcggaaga gccggatggc
    15601 ggcttaccag aaagaaggag attccggtca gcgcaaacaa aagagtttca aaccaggttc
    15661 cgacggtgcg ttcttcgcgc atgtcgatga taccgttaaa ataatccaga agccgggaca
    15721 ttccctgcgt gtagtgagca aaaaaggtct gcgcaccgac gaggaaacaa agaataatca
    15781 gcggccaaaa caggagacgg agcagttcac ggactttcat ttcaaacccc tttcatgtga
    15841 cggaatgtat acagtcataa aatattttgt caggtaaaat acggcgttgc ctgcaaatcg
    15901 cgttttcatt cgcctttgac gggtttcaaa atgatgtacg tgggcagacc catcactccg
    15961 aaatggtcga ggatttcttt ggcgggcgat tctttgtagt ttgtagcgtc gaatttaagc
    16021 accttatacg gcgtcaaccg gttctttata tccggggctt caaacgtggt gctgtccatg
    16081 gcgtggcagt tcttgcacca gtcagcccag aagtcgatca gaaccggctg accggccgca
    16141 aaggcctcgt tgagcatatc gtgaaaacag gtattctgac cgttacaaaa caccagatgt
    16201 tcttcgccgc ccggcggtgc cagacgcgct ataagaagcc ggccgcccag cattccgtaa
    16261 taaattgcca gcaccagaat cagcacaccg aacccgcgct tcacccattc catccagcgt
    16321 cccggcttgg gcaggaatga aagcccggcc ccggcaaacg gccacggcag cgccattccg
    16381 agaccaagaa caaacggcag tgccagtccg gcatgaacgc cgtttgtgta cagattgccg
    16441 gaaagcagca gcaccgcgat caccaccggc gccacacacg ctccggccag cagtgcggaa
    16501 agtccgccga tactgaaggc ggccagaaat tcttcttcga tctgccggcg gttcagatgc
    16561 cggtgcagtt ttgtcagatc aatgtgaaac acgttgaaca tggccagagc cagcgccaga
    16621 aatatcgccg cgataccgag attgaaccac gggttggcat tcagcgcccc gaattttgca
    16681 ccgcccagca caaccgccag ccccagaatt ccgtaaacaa cggtaatacc cagtccataa
    16741 accgcaccca gcgcaaaccc tttgcgacgc gactccgact gagctccggc accgataatc
    16801 gtcagattga ccgggatcat cggcaggacg cacggcgtca gattcaacgc cagtccgccg
    16861 aacagaatca gcgccagcgt cggccacatc ccccaccgct cataaaattt caccggacgc
    16921 gccgtaaaca tcttaaaaat gccgggccgt ttatgcattc cctcggcacg gtccagaaat
    16981 tccgtaaagt ccgcttcgtt catgtagccg gttccggtgc ggaccactgt gaactgagaa
    17041 accgccttcc gccaggaatt gtcggactgt gccgtctgca cggctgtctg cgctgtgccg
    17101 gtcagcgcgg tcagaagaag aaacgagaaa agaattaact ttttcatagg tacacttcag
    17161 actgcctcca tatgaaccgg cctgagcata ctgcgttaaa tatcctgaag caaatccgca
    17221 aggcggattt taccgccttt ttcgccggag gctcggtgcg cgacaaactg ctgggccgca
    17281 ccgcgaaaga ttacgacatc gccaccagcg ccacccccga ccagatcgaa gccctcttcc
    17341 cgaaaacggt ggccgtcggc aaagccttcg gcgtcatcat cgtcgtacag gacggcaccg
    17401 aaacggaagt cgccaccttc cgcgccgacg gcggctatca ggacggccgg cggcccgaca
    17461 ccgtcgaatt ctgcggcgcc gaagaagacg ccaagcgccg cgacttcacc gtcaacggca
    17521 tgttctatga cccgctggac gaccgcatca tcgactacgt cggcggacag gaagatctcg
    17581 aaaaaaaaat catccgcgcc atcggcgacc cggaccggcg cttcgccgaa gaccacctgc
    17641 gcatactgcg ggcggtccgc tttgcgcaca cactcggctt tgcaatcgaa ccggccaccc
    17701 gcgccgccat ccggcgccat gcgcccgatc ttgtaaaaat cagcgccgaa cgcatcgaaa
    17761 acgaattttc ccgcacgctc acggaaagcc tccggcccgg tgacgcgctc agagaactgg
    17821 tcgatctggg cctgatggaa tttatcatcc ctgaaatcct tcctatgatc ggacaggaac
    17881 agccgccgga attccacccg gaaggcgatg tctttgaaca taccgttctt atgctgaatc
    17941 tgatgaatca gcagaccgag atgccggcct cacaaatcgt ggagcaggct ttccagcctg
    18001 ctcatccttt ccgcccggta gaacaaaccg ccgcgctcca tacctcccgc cgccgtctgc
    18061 cccactggga gcaggactcc aggatctact ttatcacctt ccgcctggca gactcgattg
    18121 cacaggaaaa actcaatcag tggcaggaag aactggaaat ctggaagaaa caacagcccg
    18181 atcccgcttc tgaagaaaca gaacgcgaac gggcacggct ctatcatgag cgacaagaga
    18241 aatggctcga taaaggatat ggttcctgca tcttaaaaaa tcctgaaatt tccgccatcg
    18301 tggagcagac actccgccac ttcgatggcg aacgataccg gctgggtgac tacgtcatca
    18361 tgcccaacca tgtgcatttg attatcgaac ctgcagccgg acaaccgctc tccgggatca
    18421 tgcagtcatg gaaaggatat tcggcaaggg aaatcaataa agtgaccgga tccaaaggcg
    18481 ccctttggat ggacgaatcg tttgatcata ttatccgcag cgaagagtct cttgaaaaat
    18541 ttacagacta tatgcgtcaa aatcctgtcc aagcacatct tgcatccacc ggcttccggg
    18601 caggaaccgg atccctgtct gacatcaggc aggctggaaa gcctgcctca catggggagc
    18661 cgcccttccc gcccgttcat tcatccaatt tcaaaaaact gctggcctac accgccctgc
    18721 tgcacgacgt cggcaaaccc gccaccgcgt ttatgggcgc ggaccgcctg cgctttcacg
    18781 gacacgaacg caagagcgcc gaaatggccg aagaaattct gcggcgcttg aaactgccgg
    18841 tgaaagaaat ccggcggatc gtcaccgcca tcgacggaca catgcgcttc aaagacgtgc
    18901 agaagatgaa caaatccacc ctgcgcaagc tgatgggggc ggaaaccttt gagctggaac
    18961 ttgaactgca ccgtatcgac tgcgccggca gccacggcct gctcgacaac tatgacttcc
    19021 tgctcaaaaa ggccgacgaa atgaaaaacg agcccattct gccggagcgc tggatcaccg
    19081 gaaaagattt acgggaaatc ggcattgcat ccggtccgca catgggagaa ctcctccagc
    19141 tggcctacga cgcccagctc gaaggccgct tcccggaccg cgagaccctg ctcgcctggc
    19201 tgaaaacaca gctctgaaag ttttgtgttt ttcgtggcta tacgctccaa ggtttggaaa
    19261 actgcggcca cgatttaaac ggggagtgat tccatggaag agctgattcg aaaactgctg
    19321 gttgaactgg gcgaaaatcc ggaacgcgaa ggactgaaga gcacgccggc gcgtgtcgcg
    19381 gagtcctata aatttctgac gcacggctat cacctgaaca tcgacgatgt gctgaacgac
    19441 gccttcttcc aggccgaaga cagccacatg gtcatcgtca aagacatcga ggtctacagt
    19501 ctctgcgaac accacatgct gcccttcatc ggcaaatgcc atatcggcta tattcccgac
    19561 aagcgcgtcg tcggtgtcag caaactggcc cggctggtgg atgtcttcgc ccgccgcctg
    19621 cagattcagg agcggctgac cgatcagatc gcccacacga ttatggaaaa gaccggcgcg
    19681 cagggcgtcg gcgttgtcat cgaagcccag cacctctgca tgatgatgcg cggcgtggaa
    19741 aaacagaatt ccaagatgat cacgtccgcc atgcacggca ccttccgctc gcagctggca
    19801 acgcgcaccg agtttctgcg gctgatcggc accatcggct aacggagcaa ggcaaccgag
    19861 acggttgcga tacgtaccga cagcctcgtc tctttgtatc gccggcttcc agccggcctt
    19921 tagtcttaac gtgccttctg tttcccgctt gtcggaacag accgaaactg gaaagatgcg
    19981 cggcactatg ctgagccgtt tgaacatccg caacctcgcg ctggtcgaca acatcaccgt
    20041 gtcattccag agcggcttta ccgtcatcac cggcgagacc ggcgcgggca aatccctgct
    20101 catcggcgca ctgcgcctgc tgctgggcga gcgtgccgac aagagcatca tccgcaccgg
    20161 agagacatcg tgcagcatcg aagcggtctt tgaactgcgc gatccgtctg ccgtcaacag
    20221 cgcgctcgaa gaggtcgggc tggcgccctg cgatgaaggc cagctggtgg tccgccgcac
    20281 catcaccgaa agcagctcca aaaatatcgt caacgactcg cccgtcaccc tgcccgtcct
    20341 caaagcgctc ggcgacgtac tggtcgatat gcacgggccg tacgaccacc agtcgctgct
    20401 gaaaaccgac gcccagatgg acatactcga cgccttcggc cagctcgccg ccgaccgcgc
    20461 ggcctatcag gaaaaataca aacggttcca ggcgctgaaa aaccggctca atgaactttc
    20521 aaacctcgac gactccggtc ttgccgaaca gatcgacctg ctcgcctggc gcgtcaaaga
    20581 aatcgaagaa gccaaaatct ccgaagagga cgaaaccgcg gtacagcagg aacacgaaca
    20641 gatcgccaac gcccagaaca ttctcgaact ggcgcaaacc gcggtaggcg gattgaccga
    20701 agccgagtat tccgccatcg aaggactctc caccgcccgc aaagcctgcg cgcagctggc
    20761 gaaatatctg cccgacgccg aaacgtgggc cgaagaactc gaaggcgcgg cgcgcaccgc
    20821 gcaggaaatt tccgccgcca tccagagcgc agttgaagag gtcgacgccg ggccggaacg
    20881 gttgcagttt ctcgacgaac gcctcgcgct ctaccgcaac ctgaaacgca aaatcgcccc
    20941 cgccgtcccc gaaatcctcg ccaaatgcga cgagtggaaa gaaaagctga aggagttgca
    21001 atcgcgcgat gaacagcgcg ccgccgtcag cacggcgctc gacgccgcac tcatcgaaac
    21061 cgaacaggcc ggacgcatcc tgcgcaaaaa acggcagaaa gtcgccggcg acctggccga
    21121 cgccatcacc gccgaactgc gcgacctcgg cttcgaacac ggactttttg aggtccaact
    21181 gctggatgcc gacgaaccgt gcgcctccgg cctcgacgaa atcgaattcg gcttcgcgcc
    21241 caacgccgga gaatccatgc gcccgctgcg ggccatcgct tccagcggtg aaatttcgcg
    21301 cgtcatgctc gccgtcaaag ccgtactggc gcgccacgac cggattcccc tgctcgtctt
    21361 tgatgaaatc gacgccaacg tcggcggcga aatcgccaac gccgtcggcg acaagctggc
    21421 gcaggtcggc cggacgcacc aggtcatcgt cattacgcac ctgccgcagg tcgccgtctg
    21481 cggacagacc catttttccg tctcgaaaaa agtacaggac ggccgcacct tcacccagat
    21541 cgctgcactg accggcggca cccgcgccga agaaatcgcc cgcatgctcg gcggcaa
//
LOCUS       DAOPZO010000016        21504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_2503_length_21504_cov_9.331764, whole genome shotgun sequence.
ACCESSION   DAOPZO010000016 DAOPZO010000000
VERSION     DAOPZO010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 21504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21504
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2503_length_21504_cov_9.331764"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..673)
                     /locus_tag="PKI68_02225"
     CDS             complement(176..673)
                     /locus_tag="PKI68_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52540.1"
                     /translation="MNFKIRWLLPFRGGGKRKYTALLFILVCVLAMIFVVIKRPERNS
                     ILYYESIRGHGMIPFSPWNEISEAEAEKLLAYFKGYYDAERRLFKFEQYLRTEKHTFQ
                     KIFEDEYFYNDKGKIYKVLSKGEGLAAFYISASDSNPDGVVFILDAKGRIVSATKTNG
                     EKLSL"
     gene            complement(777..1826)
                     /locus_tag="PKI68_02230"
     CDS             complement(777..1826)
                     /locus_tag="PKI68_02230"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNX52541.1"
                     /translation="MNNELSLSQNLFSEVRSLIEQGRQQVAVTVNAAMTLLYWQVGKR
                     INDEVLKDRRAEYGQHVVAALSKQLTAEYGKGWSKRHLHHCIRFAETFPDPEIVNALR
                     TQLSWTHIRTILPIEDELKRAFYIEMCRLEKWSTRTLEERIQSMLYERTAISKKPEET
                     IQRELQQLGATGQITPDLVFRDPYFLDFLDLKDSYSEKDLESAITAELQRFITELGGD
                     FAFMARQKRIPIDSRDYYLDLLFYHRRLKALVAIELKLGEFDAAYKGQMELYLAWLEK
                     HESVEGENPPIGLILCAGKNPEHVELLQLHKSNIKVADYFTILPSKEVLLDKYHKAIA
                     IARNSMALRGEREES"
     gene            complement(1905..2576)
                     /locus_tag="PKI68_02235"
     CDS             complement(1905..2576)
                     /locus_tag="PKI68_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52542.1"
                     /translation="MPNIESDPFLARIQTVHPDGSPAIAPYIALTDKELRFHQGVGIM
                     YAAIKGGEIVKIYKQPPIKRDDVSREQAIKNAGTKGFCKSAKIEYDRLIRGSQIKEVY
                     RKQAWHKHLINHGCELWRGTFTTDHFCKPEKITYVEILPVELPKDQADRLGQIADDLH
                     LLHKNLKESHLSMRPCYWETSSNFKTLSTLEMSAKHLNDFLKYMNQQESNTDLPMSEW
                     LDPEW"
     gene            complement(2607..3638)
                     /locus_tag="PKI68_02240"
     CDS             complement(2607..3638)
                     /locus_tag="PKI68_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52543.1"
                     /translation="MSTSFANVPLQWVGPVKITGPEIDVETELPLATYETPLFASVNR
                     GARVATASGGIKAVVIDERMSRSVLFEAPDAIVAFQCLEKIKAEFSTFGKTVATTSRF
                     ARLIDMNSQIAGNLLFIRFEFTTGDASGHNMVTFASEALVNHILKLCPALKYESVSGN
                     FCSDKKATAVNGILGRGKYVIAELTIPEKLVQKYLKSTPEKIVQLNIRKNLIGTMLAG
                     GLRSANAHYANMLLAFYLATGQDAANIIEGSQGVTQAEVRNGDLWFSVTIPNLIVGTV
                     GNGKGLPFVEEVMQKLGCRVEREPGANARRLALICSAAVLCGELSLMAALTNPGELMA
                     AHRKLERSS"
     gene            complement(3690..4616)
                     /gene="cysK"
                     /locus_tag="PKI68_02245"
     CDS             complement(3690..4616)
                     /gene="cysK"
                     /locus_tag="PKI68_02245"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNX52544.1"
                     /translation="MKIYDNVTQTIGGTPLVRLNRLTAGLKAVVAVKMESRNPLGSVK
                     DRIGIAMIEAAEKSGEIKPGATIVEPTSGNTGIALAFAAAAKGYKLILTMPETMSIER
                     RKLLAALGAELILTPGSEGMPGAIKKAEALVGSLPNAFMPQQFDNAANPEIHRRTTAV
                     EIWNDTDGKVDLFVAGVGTGGTLTGVGSVLKKKKPGVKVVAVEPADSPVISGGKPGPH
                     KIQGIGAGFIPTNLDTNLVDEVIQISAADAGITARRLAKEEGILCGISAGGNVWAAIQ
                     LAKRPENEGKLIVTVICDTGERYLSTWLFDEA"
     gene            complement(4634..5077)
                     /locus_tag="PKI68_02250"
     CDS             complement(4634..5077)
                     /locus_tag="PKI68_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNX52545.1"
                     /translation="MKMSTKGRYAVRILLGIARYQKNGPVSKKIIAEEESISADYIEQ
                     IIVPLKNEGLVVGLRGVKGGFRLAKDPAKITVYDILRASEGELSLVDCERNDCKGRDK
                     NICVTRPVWEGASKVLEAYFSKITLKDLLDRVADLEKSGAASYTI"
     gene            5253..6905
                     /locus_tag="PKI68_02255"
     CDS             5253..6905
                     /locus_tag="PKI68_02255"
                     /inference="COORDINATES: protein motif:HMM:NF011291.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNX52546.1"
                     /translation="MSSSPGNFIHDIIDADLASGKHTEVVTRFPPEPNGYLHIGHAKA
                     ICLDFGTALKYSGRCHLRFDDTNPTAEDTEYVEAIENDVRWLGFDWGKNLFWASDYFD
                     RMYECAVELIKLGKAYVCDLSVDAFKEYRGVPNLPGKEPAGRKRSVEENLDLFERMKK
                     GEFADGTYVLRAKIDMASPNLHMRDPAIYRIKHASHHNTGDKWCIYPMYDFAHCLEDS
                     IEGVTHSLCTLEFEVHRPLYDWILETLGLYQPHQYEFARLNLTYTIMSKRKLLELVKE
                     KLVNGWDDPRMPTICGMRRRGYTPEAIRAFCTEIGITKTESLTDVALLEHHVRNDLNE
                     IAPRRMAVLDPLKVVITGYPEGQTEEFELPNHPNKPEMGVRTVPFSREIYIEREDFME
                     EPAKKFFRLAPGTEVRLRGAYLVTCTNVVKDAGGSITELHCSYDPASKGGNAPDGRKV
                     KGTIHWVSAQHGIQAEVRLYDRLFTSENPAAEEGDFKDYLNPDSLKVITGIIEPGLAA
                     AKAGEKFQFERIGYFCADLDTSAGKPVFNRTAGLRESSAKQA"
     gene            6947..8149
                     /gene="dinB"
                     /locus_tag="PKI68_02260"
     CDS             6947..8149
                     /gene="dinB"
                     /locus_tag="PKI68_02260"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNX52547.1"
                     /translation="MTGNSQKTILHIDMDAFFAAVEVHDHPELKGKPVVIGSPPDKRG
                     VVSTCSYEARKFGIHSAMPSRTAFKLCPHAVFLPVNGRRYREVSEQIMSVFHRFTPLV
                     EPLSCDEAFLDVTGARYLFGDGPEIARKIKAAVREETGLTCSVGVASNMFLAKIASDL
                     HKPDGLTVVPDSEKGIAAFLAPLPIKRMWGAGKKTQATLEAHKIRTIGDLQTVDPAKL
                     AAWVGESSAASFRRLAFGLDDREIETASEEKSISNEITFDEDQRDAAIVEAALLDLAD
                     KVGTRLRRAGFYAATVQIKLRWKDFSTITRQRQLDPPCCDDITLRETALELFRKETLH
                     SPVRLIGFGASGLRRTADAPQLDLFQSPGKKTSARREALSRAVDRIRSRFGGNSIRRG
                     SNLKQEDN"
     gene            8226..9065
                     /locus_tag="PKI68_02265"
     CDS             8226..9065
                     /locus_tag="PKI68_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNX52548.1"
                     /translation="MNPLFQSPLTCPSCETALEWNEKECRCANGHSFDLAKEGYANLL
                     LSHQRRSSHPGDDDKMIQARRRFFDSGAFEPVTDQIKKSVIGNHQSAMSILDCGCGEG
                     HFLGALSEAHAGQFYGVDVSKEAVRCAAKRYKKAKWIVANGMRKLPFADHSLDVILSV
                     LAPRNPEEFARILKPDGTLILGVPGPNHLIELRSRLAANAGDFEEKADEAAAKCAPHF
                     KETHRELLSYQQTLNAAQLADLIQMTPIFWCSSPEAKADVQRLSELTVTVSFVLLALQ
                     PSS"
     gene            complement(9516..10811)
                     /locus_tag="PKI68_02270"
     CDS             complement(9516..10811)
                     /locus_tag="PKI68_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNX52549.1"
                     /translation="MSLDSKSVEERLASVKALGESIKAGVKQTDEVNNHVHSTYSFSP
                     YSPAEIPARAKEAGLGTVGIMDHDSVSGCEEFLNAAKAIGIAATAGCEIRVNMDGTLV
                     EGRKTNNPDEPNISYIAFHGIPRNQFAAVTEFLKPITMARIARDRKEVERLNAKLAQL
                     GAPQLDFEKDVMAISEAANGGSITERHILYALSLKLIAKLGKGAALVAFVEAQMQIPV
                     PGKLKAVLLDLNNPHYAYDLLGVFKSALVPDFFILSGKDECINVRDAVKFANDIGAIP
                     AYAYLGDVGESPTGDKKAEKFEDDYLDALVPELKKLGFKAITYMPPRNTRPQLLHLQR
                     LCQANGLMEISGVDINSSRQSFNCPILLEPEFRHLVDAAWALIAHEKLAAENPKLGLF
                     HPQNPLAAQPLEKRIQKYAAIGRQIDHRQPEMALRLVEF"
     gene            complement(10879..13332)
                     /locus_tag="PKI68_02275"
     CDS             complement(10879..13332)
                     /locus_tag="PKI68_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNX52550.1"
                     /translation="MPKHQIVCPREVRKAGEITFKPIPVNQYKPDYKKEVKKYGGERL
                     KKMYYDMLVVREFETMLNQIKVQGSYAGIEYNHPGPAHLSIGQEAAAVGQAVNLTTDD
                     CIFGSHRSHGEILAKCLSAVYQLDDAKLTKIMDEFFGGSTVKVVKDGSQKSVKDLAEE
                     FVLYGTLAEIFARQTGFNKGLGGSMHAFFIPFGSMPNNALVGGSGDISVGSALFKLIN
                     RKPGIVIANIGDASLGCGPVWEGFWMAAMDQYRTLWDKSLGAPPVLFNIMNNHYGMGG
                     QTAGETMGYGMVARIGAGINPEQMHAERVDGYNPLAVADATERQKKILLEGKGPALLD
                     VVTYRISGHSPSDASSYRSKEEIESWEANDCVKLFGEYLISNGALTADEAAALKATVV
                     AKVTKTLRLATDKTVSPYTDINIIEDVMYSNGKVEKFDDRKPDVLMPMAENPRVKKLA
                     GKARYAFDENGKPIGANKLYTFRDGIFEAMLHRFYTDPTMIAYGEENRDWGGAFACYN
                     GLTESIPYHRLFNSPISEGAIVGSACGYALSGGRVCVELMYCDFMGRAGDEIFNQISK
                     WQSMSAGILKMPLVLRISVGNKYGAQHSQDWAAMVNHIPGLKVYYPATTYDAKGMLNL
                     ALAGTDPVIFFESQKTYGIGEMFEKSGVPEGYYETPEGEPAVRIQGKDLTIITVGPVL
                     YTGKKAVETLKAQYGLEAELIDLRFINPLNYDRIVESVKKTGRVVLASDSVERGSALH
                     NIAANITQLCFDRLDAPPVVVGAKNWISPAAEMETDYYPQADWLIDAIHQRILPLPGH
                     VAKHNYTAGEMLRVARKGV"
     gene            complement(13350..14690)
                     /gene="lpdA"
                     /locus_tag="PKI68_02280"
     CDS             complement(13350..14690)
                     /gene="lpdA"
                     /locus_tag="PKI68_02280"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNX52551.1"
                     /translation="MYDIIIIGAGPGGYVMAERAGHLGKKVLLIEKEHLGGVCLNWGC
                     IPTKTLLNSAKHYVHAKEAPEFGVTTGEVKFDLTKAMAWKQEVIETLRGGIGFMMKKN
                     KVTVVNGTAKLLGPRKVQVGETVYEGTNVIIATGSSPFVPPIPGAKQPHVMTSTEILN
                     IDKMPETLTVIGGGVIGVEFASFFSSLGVKVDVIEMLEEIIPFMDRGQAKEFRRALKG
                     KVNFNLGCKVTAIDGHDVKYTDAKGQSQSIQADIVLMSVGRVPNLAGMGFEEARLDFD
                     RRGIKVDEQMRTNLPDVYAIGDVTGLSQLAHSATRMGEVALNTICGQPDRFRRNAIPW
                     AVYSLPEVAGCGLTEEQAQAAGHKVQTASLPLTMSGRFLAENGKRGSGSVKVIKDAGT
                     DVLLGVHMVGAGVSEMIYGAAVMIEAELRVKDIREIVFPHPTVGEVVRDTMFQF"
     gene            complement(14727..16046)
                     /locus_tag="PKI68_02285"
     CDS             complement(14727..16046)
                     /locus_tag="PKI68_02285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNX52552.1"
                     /translation="MATEVLMPRQGQSVESCIITGWKVKEGDPVKAGQPLCEVETDKA
                     AFEVEAPAAGTVLGVFYPDGADVPVLRIIAAIGAPGEDISALRPSAAAETKPEAGSKA
                     EEKPSVSNPAVSSFKFQSSGLSSGVSPRARNLAAAEGLDVSALAGSGPEGRVIERDVK
                     AALAGQAPLSPAAKAKIAAIGSGIGGRILAADVQMPSAAVPAAPAVSYSAEQMGGLKE
                     IPVKGVRKVVAGRMLSSLQTTAQLTMHTSADARAVLSARAKFKTSADKAGITINDLVL
                     YAVAQTLVNFPDLNAYWLGDKLVQFENVHLGMAVDTPRGLMVPVIRFANKLSLEEISV
                     EAKRLGRACIKGTIDPDALKGGTFTVTNLGASGIEMFTPVLNAPEAGILGVCSVQPKP
                     VMKKDGGVEFIPHMGLSLTFNHCATDGAPAGRFLAALREKLLSFEIQ"
     gene            complement(16059..16844)
                     /locus_tag="PKI68_02290"
     CDS             complement(16059..16844)
                     /locus_tag="PKI68_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HNX52553.1"
                     /translation="MNKKKESELSAQFPDRKKAILDMLAEDSRASVSILAQKLGVSVV
                     TIRTDLAALEEEGFIVRTHGGAMPAFHPQMLERMRQEKDRKAAIARFAAEKIQDGDTV
                     IISAGTTTALIAKYLLGRRNVHIVTNNTLLLTYARANPQLRVTLVGGEFRPSEEGLVG
                     PLAIQSLDQVFVSKAFIGVDGVSIKQGLTAHLVESADLVRKMAQQAEQVFAICDSTKF
                     NKAGFARILPFNGVNGLITDSGLDVDVEKELREAGVSIYKAEG"
     gene            complement(16900..17751)
                     /gene="prmC"
                     /locus_tag="PKI68_02295"
     CDS             complement(16900..17751)
                     /gene="prmC"
                     /locus_tag="PKI68_02295"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNX52554.1"
                     /translation="MIRQYEMRLAAAGSGNAKRSVEELAAHVLGCKPLEIYFRKITPE
                     QEAELEKLIVRAEQSEPIQYIIGHVDFRGLEIRCDRRALIPRPETELLVDAVLSSIGN
                     RQSSMIIADIGTGTGCIALALLSELPNAEVLGIDLSPAALALARENAKRLGLIDRFSS
                     LENNLLEGVEVNSLDIAVSNPPYILSDVCKTLDPSVRDFEPQLALDGGRDGLDLIRIL
                     VEQAARVLKPGGGLFLEIGYDQGPAVRRILQKAGFEQTEVKKDLAGHDRIVLGRMPGG
                     PVPAAII"
     gene            17925..18518
                     /locus_tag="PKI68_02300"
     CDS             17925..18518
                     /locus_tag="PKI68_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52555.1"
                     /translation="MIDINTFEEVRQRFGHFASWAVWAESGGSLKDNIADLTVFDPES
                     NPHLLQTLHVNAILIGLNISRPIERPLGNFHDPRPMATDFKIRYALYGTPYWGAYMTD
                     IIKDFEEKVSGKMVGFLKAHPDFERDNIKQLKAEIDVLGCSNPVLIAFGKDAERIARR
                     NLGDEYSIVGIPHYANYISKEDYRNQVWDVLQCQGRK"
     gene            18677..19537
                     /locus_tag="PKI68_02305"
     CDS             18677..19537
                     /locus_tag="PKI68_02305"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNX52556.1"
                     /translation="MKNGQRITVFMLTLLLSCGLSAQDNSSPQDWQIPAQKENFHIFL
                     LMGQSNMAGFGEMLPEDTQPVPRVLKLPTIGEVQWEPAAHPLHNRQKSDRFGLGLPFA
                     EEYLKDKPGVVVGLIPVAQGGAAIDKMNKGTPIYADAIKKAEFAATQGVIKGVLWHQG
                     ESDTTSAPRTDIYEQKLHQLIADLRQDLKDDNLPFIAGNLAEFYGTGPEHNKPDRVAR
                     IDMVRATLRALPDKVKNTGFAESTGCSSADAHMVHFDRNSYIILGKRYAEVYSRIINK
                     ELKTPGQLAP"
     gene            complement(19589..20734)
                     /locus_tag="PKI68_02310"
     CDS             complement(19589..20734)
                     /locus_tag="PKI68_02310"
                     /inference="COORDINATES: protein motif:HMM:NF002326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNX52557.1"
                     /translation="MTHARQEWEKLESRDLAPYAAHSARSLGRLYPEDDHPVRPCFQR
                     DRDRVLHSRCFRRLEYKTQVFVNGTADHYRTRLTHTIEMAAVGRTLARAFRVNEDLTE
                     TICLAHDVGHSPFGHRGEYVLDELMKDHGGFDHNLQSLRAVEKLEYQYPGFPGLNLTW
                     EVRAGLLKHQAAKPGGQLDGHPIGPFQYLEAQIADVADDVTYHAHDVDDGLEAGLITL
                     EQLEKLAFWKLAAARTREQYAALDDGHFLRITIRNLLDLQAQDVLREGRRRIERHNPR
                     AIADVMNAPERLVAFGDEMGGLLDEFSKFMYRQLYEHPAVLNANHEAVALMRGLFLHY
                     IEHPETMGRKALARVAEEGLRRTVCDYVAGCTDRYALEECQKYGIGA"
     gene            20899..21423
                     /locus_tag="PKI68_02315"
     CDS             20899..21423
                     /locus_tag="PKI68_02315"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNX52558.1"
                     /translation="MHEKTIGSKTVFKGRILSVDVLDVELADGRTGTREIVRHGAAVA
                     VIARRRDGRFVFIRQFRKAMERVCFEVMAGNCDPDEAAEAAAVRELKEETGYAPDSIR
                     FLSSIYPSVGYCTERIDIFYAEVSEPGETDFDHDENIETVLLTKDEMDAMIRAGSVQD
                     AKTLAAWMLYTVYM"
BASE COUNT         4695 a         6175 c         5732 g         4902 t
ORIGIN      
        1 cgaccgacgg atcaatcgcc ttcaggtcga tttccggcag cgcacggtgc gtcagtcgga
       61 tttcgtcgaa gtaaaactta cggcgatcca tctcgccttt ctccgcgacg atcttaacgt
      121 gctccacttt ccgtttttca agttcactca tttagacaag ccccttagat tcgacttaca
      181 gagaaagctt ttcaccgttt gttttagttg cacttactat gcgtcctttt gcatctaaaa
      241 taaaaacaac cccatctgga ttgctgtctg acgcggaaat gtaaaaagca gccaaccctt
      301 ctcctttcga taagacctta taaattttcc ccttatcgtt atagaaatat tcgtcctcga
      361 agattttctg aaacgtgtgt ttttctgttc tcagatattg ttcgaattta aataatcgtc
      421 gttctgcatc ataatatcct ttaaaatagg ccaatagttt ttcggcttct gcttcgctga
      481 tttcgttcca cggtgaaaat ggaatcattc catgtccacg aatagactca taataaagga
      541 tggaattcct ttctggcctt tttatcacca caaatatcat tgccagcacg caaactaata
      601 taaataacaa tgcagtatac tttcttttac cgccccctct aaaaggaagt agccaacgaa
      661 ttttaaaatt catgctcacc cctttctttc cagcttacgg tgggcggcca tcagctcgcc
      721 ggagttggtg agctcggtga tcttgacgtg ctccactttc cgtttttcaa gttcgctcat
      781 gattcttccc tttcgccccg cagagccatg ctgttgcggg cgatggcaat cgccttgtga
      841 tatttatcaa gcagcacctc tttagacggc agaatggtaa agtaatcggc cactttgata
      901 ttgctcttgt gcaactgcaa cagttcgaca tgctccggat tctttcccgc acatagaatc
      961 agtccgatgg gcggattctc accctcgacc gattcgtgtt tttcgagcca cgccagatac
     1021 agctccatct ggcctttata ggccgcgtcg aactcgccga gcttcaattc aatcgccaca
     1081 agcgccttaa gccgacggtg ataaaaaagc aaatccagat aatagtcgcg actgtcaatc
     1141 gggatacgct tctgccgggc cataaaggcg aagtcgccgc ccagctcggt gatgaaccgc
     1201 tgcaactcag cggtaattgc cgactcaagg tctttctctg agtagctgtc cttcaaatcg
     1261 aggaaatcca gaaaatacgg atcgcgaaac accaagtcgg gagtaatctg acccgtggct
     1321 cccagttgct gaagttcacg ctgaatggtc tcttccggtt ttttactgat cgctgtgcgc
     1381 tcgtaaagca ttgattgaat gcgctcctca agcgtccgcg tactccattt ttcaagcctg
     1441 cacatctcaa tatagaacgc ccgcttcagc tcgtcttcga tcgggaggat ggttcgaata
     1501 tgcgtccagc tcaattgtgt acgcagtgcg ttcacaattt caggatcggg aaaagtctcc
     1561 gcaaagcgga tacaatgatg caaatgccgc ttgctccagc ctttaccgta ctccgccgtc
     1621 aattgcttcg acaatgcggc gacaacgtgt tgtccgtatt ccgcccgccg gtccttcagc
     1681 acctcgtcat taatccgctt accgacctgc cagtacaaca acgtcatcgc ggcgttaacc
     1741 gtcaccgcaa cctgctggcg gccctgctca atcagcgatc tgacttcgga aaaaaggttc
     1801 tggctcaggc tcagctcgtt attcatgagt tgtccttctg cgcgacgatc ttaacgtgct
     1861 ccactttccg tttttcaagt tcgctcataa aaattcctga caggctacca ttctggatca
     1921 agccattcgc tcataggcag atcggtgtta ctttcttgtt ggttcatgta tttgaggaaa
     1981 tcatttaaat gctttgcaga catttccaaa gttgaaagtg ttttgaaatt actcgacgtt
     2041 tcccagtagc aaggccgcat tgacaggtgg ctttccttta ggtttttatg cagcagatgt
     2101 aagtcgtctg caatttgtcc taatctgtcc gcttgatcct ttggaagttc aactggcaga
     2161 atctcgacat aggttatttt ctccggcttg cagaaatgat ccgttgtgaa tgttcctctc
     2221 cataactcac agccgtgatt aatgagatgc ttatgccatg cctgttttcg gtaaacctct
     2281 ttgatctgcg agcctcttat caatctgtcg tattcgattt tggcagattt acaaaagccc
     2341 ttagttccgg cgtttttaat agcttgctca cgggaaacat cgtctcgttt aataggaggt
     2401 tgtttataaa ttttaacaat ctcaccacct tttatggctg cgtacataat gcctacgccc
     2461 tgatgaaacc gtagctcttt atcagttaag gcaatataag gagcaatggc cggcgatcca
     2521 tccggatgca ccgtttgaat ccgagcaaga aatggatcgg attcgatatt tggcatatta
     2581 acttcggaat tcgcgctcat tcgtggttaa gaacttcttt ctagtttacg gtgggcggcc
     2641 atcagttcgc cgggattggt gagtgcggcc atcaaggaaa gttcgccgca gagaacggcg
     2701 gcggagcaga tgagcgcgag gcggcgggcg ttggcacccg gctcacgttc aacgcggcaa
     2761 ccgagtttct gcatcacttc ttcgacaaac ggaagccctt tgccgttgcc gaccgtgccg
     2821 acgatcaggt tgggaatggt gacggaaaac cagaggtcgc cgttgcggac ttcggcctgc
     2881 gtgacgccct gcgatccttc gatgatgttg gcggcgtcct gtccggtggc gagatagaag
     2941 gccagcagca tgttggcgta gtgcgcgttg gcggagcgca gtccgcccgc cagcatcgtg
     3001 ccgatcaggt ttttgcggat gttgagctgg acgatttttt ccggcgtgct tttcaggtat
     3061 ttctgaacga gcttttccgg aatcgtcagt tcggcgatga catatttgcc gcgaccaaga
     3121 ataccgttga ccgccgttgc ctttttgtcg gagcagaagt tgccggaaac cgattcgtat
     3181 ttcagtgcag gacaaagctt gaggatgtgg ttcaccagcg cttcgctggc gaaggtgacc
     3241 atgttatggc cggaagcgtc gccggtggtg aattcaaagc ggatgaaaag caggttgccc
     3301 gcgatctgcg agttcatatc gatcagcctg gcgaagcggc tggtcgtagc cacggttttt
     3361 ccaaaggttg aaaattccgc tttgattttt tccagacatt ggaaagcgac gatggcgtcc
     3421 ggcgcctcga atagaaccga acggctcatc cgctcgtcga tgacgacggc tttgattccg
     3481 ccggaagcgg tggcgacccg cgcgccgcgg ttgacggagg caaagagcgg cgtttcgtag
     3541 gtcgccagcg gcagttcggt ttcgacatca atttccggcc cggtgatttt taccggcccg
     3601 acccactgaa gcggcacatt ggcaaaagac gtactcatag gctggtgttt tagcctgttt
     3661 cgccgccgcg caaaaggttt ttccagcgct tacgcctcgt caaacagcca ggtggaaagg
     3721 tagcgctcac cggtgtcgca tatgacggtg acaatgagtt taccttcgtt ctccggacgc
     3781 ttcgccagct ggatcgccgc ccagacgttt ccgccggcgg aaattccgca caggatgcct
     3841 tcttctttgg caagacggcg ggcggtgatg ccggcatcgg cagcggaaat ctgtatgact
     3901 tcatcgacca gattcgtgtc gagattggtc ggaatgaagc ccgcaccgat tccctggatt
     3961 ttatgcgggc cgggcttgcc gccggaaatc accggcgagt cagcgggttc aaccgcgacc
     4021 actttgacac ccggtttttt cttcttgagc acactgccga caccggtgag cgttccgccg
     4081 gtgccgacgc ccgcgacaaa cagatctact ttgccgtcgg tgtcattcca gatttcaacc
     4141 gccgttgtcc ggcggtggat ttcgggattg gcggcgttat caaactgctg cggcatgaag
     4201 gcattcggca gcgagccaac cagcgcttcc gcttttttga tcgcgccggg catgccttcg
     4261 ctgccgggcg tcagaatcag ttcagcgcca agcgcggcaa gcagcttgcg ccgttcaatg
     4321 ctcatggttt ccggcatggt gagaatcagt ttgtaccctt tggccgccgc ggcaaaggcc
     4381 agcgcgatgc cggtgttgcc gctggtcggc tcgacaatcg ttgcgccggg tttgatttcg
     4441 ccgctttttt cagcggcttc gatcatcgca atgccgatgc ggtctttgac actgcccagc
     4501 ggattgcgcg attccatttt tacggctacg acagccttca gcccggcggt cagccggttg
     4561 agtctgacga gcggagttcc gccgatggtc tgcgttacat tgtcgtaaat tttcatttta
     4621 acttccttag cgtttaaatg gtgtagctgg ccgcgccgga tttttcgagg tcagcgaccc
     4681 ggtccagcag atctttgagg gttattttgg aaaagtaggc ctcgagtact ttggaggcgc
     4741 cttcccagac cggacgggtc acacagatat ttttgtcgcg gcctttacag tcgttgcgtt
     4801 cgcaatccac cagcgagagt tcaccttcgg aggcgcgaag aatatcgtag accgtgattt
     4861 tcgccggatc tttggcgagc cggaagccgc ctttgacgcc gcgcaagcca acaacaagtc
     4921 cttcattttt gagcggcaca atgatctgct caatgtagtc ggcagagatt gattcctctt
     4981 cagcaataat ctttttagaa accggtccgt ttttctggta gcgggcaatg cccagcagaa
     5041 tacgaacggc gtagcggcct ttggttgaca tcttcataac tttatcccct tccgattaaa
     5101 tcaactaatc attatcagat ttctaggttt tattaaatac gggcatatac agtcaacttt
     5161 aaattacctg ctgacctgcc cgatattacg cacaaagtgt tgccattcac gtcaaaatcc
     5221 ggcagaaaaa gcctcacttt taaggaaata ctatgagttc ttcgcccggc aatttcattc
     5281 atgacatcat cgatgcggat ttagcctccg gcaaacatac cgaggtggtc acccgcttcc
     5341 cgcccgaacc caacggttat ctgcacatcg gccacgccaa agccatctgc ctcgacttcg
     5401 gcaccgcgct caaatacagc ggacgctgcc acctgcgttt tgacgacacg aacccgaccg
     5461 ccgaagatac cgagtatgtg gaggcaattg aaaacgacgt tcgctggctc ggcttcgact
     5521 ggggcaaaaa tctcttctgg gcgtcggact atttcgaccg catgtacgaa tgcgccgttg
     5581 aactgatcaa actcggcaag gcttacgtct gcgacctgag cgtcgatgcc ttcaaagaat
     5641 accgcggcgt gccgaacctg cccggcaaag aaccggccgg ccgcaaacgc tccgtcgaag
     5701 aaaacctcga cctgttcgag cggatgaaaa aaggcgaatt cgccgacggt acatatgttc
     5761 tgcgcgcgaa gatcgacatg gcttcaccca accttcacat gcgcgacccg gccatctacc
     5821 gcatcaaaca cgcttcgcac cacaataccg gcgacaaatg gtgcatctac ccgatgtacg
     5881 acttcgccca ctgcctcgaa gactccatcg aaggcgtcac tcactcgctc tgcacactgg
     5941 aatttgaagt gcaccgcccg ctctacgact ggattctcga aacactcgga ctctaccagc
     6001 cgcaccagta cgaattcgcc cgcctcaacc tgacctacac catcatgagc aaacgcaagc
     6061 tgctcgaact ggtgaaagaa aaactcgtca acggctggga tgatccgcgc atgccgacca
     6121 tctgcggcat gcgccgccgc gggtatacgc cggaagccat ccgcgctttt tgcaccgaaa
     6181 tcggcatcac caaaaccgaa agcctcaccg acgtcgccct gctcgaacac cacgtccgta
     6241 acgacctcaa cgaaatcgcg ccgcgccgca tggccgtgct tgatccgctc aaagttgtca
     6301 tcaccggtta ccccgaagga cagaccgaag agtttgaact gcccaatcat ccgaacaaac
     6361 cggaaatggg cgtgcgcacc gttccgttca gccgcgagat ttacattgag cgcgaggact
     6421 tcatggaaga accggcaaaa aaattcttcc gtctcgcccc cggcactgaa gtccgcctgc
     6481 gcggcgccta cctcgtcacc tgcaccaacg tcgtcaaaga cgccggcggc agcatcaccg
     6541 aactgcattg ctcatacgac ccggcatcca aaggcggcaa cgcgccggac ggacgcaaag
     6601 tcaaaggcac aatccactgg gtctccgcgc agcacggcat tcaggccgag gtgcgcctct
     6661 acgaccgcct cttcaccagc gaaaaccccg ccgccgaaga aggcgacttc aaagactatc
     6721 tgaatcctga ctcgctgaaa gtgattaccg gaatcattga acccggactg gcggccgcca
     6781 aagccggaga aaaattccag ttcgagcgga tcggctactt ctgcgccgac ctcgacacat
     6841 ccgccggaaa gccggtcttt aaccgcaccg ccggtttgcg tgaatcttcg gccaagcaag
     6901 cctgaggtcg cttccaaata tcggaaaaac agagtatctt tttccgatga ctgggaactc
     6961 acagaaaacc atactccaca tcgacatgga cgcgtttttc gcggcggtcg aagtgcatga
     7021 ccatcccgaa ctgaagggca agccggtcgt gatcggttcg ccgccggaca aacgcggcgt
     7081 cgtttccacc tgttcctacg aagcgcgcaa attcggcatt cactcggcca tgccgtcgcg
     7141 cacggctttc aaactctgcc cgcacgccgt cttcctgccc gtcaacggca gacgctaccg
     7201 cgaagtttcg gagcagatca tgagtgtttt ccaccgcttc actccgctgg tcgaaccgct
     7261 ctcgtgcgat gaagccttcc tcgacgtcac cggcgcgcgc tatctcttcg gcgacggccc
     7321 cgaaattgcc cgtaagatca aagccgccgt gcgggaagaa accgggctca cctgctcggt
     7381 cggcgtcgca tcgaatatgt tcctcgccaa aatcgccagc gacctccata agcccgacgg
     7441 tctcaccgtc gtaccggatt cggaaaaagg tatagccgcc tttctcgcgc cgctgcccat
     7501 taaacggatg tggggcgcag gaaaaaagac gcaggccacc ctggaggcgc ataagatccg
     7561 caccatcggc gacctccaga ccgttgatcc ggcgaagctc gccgcatggg tcggcgaaag
     7621 ttctgccgcc agcttccggc ggctggcttt cggcctggat gaccgggaaa tcgaaaccgc
     7681 gagcgaagaa aaaagcatct ccaacgaaat caccttcgac gaagatcagc gcgacgccgc
     7741 aatcgttgaa gccgctctgc tcgaccttgc cgataaagtc ggcacacggc tgcgccgcgc
     7801 cggattttat gcagccaccg tgcagatcaa actccgctgg aaagatttct ccaccatcac
     7861 ccgccagcgg cagctcgatc cgccctgctg cgacgacatc accctgcgcg aaaccgcgct
     7921 cgaactgttc cggaaagaaa ccctgcacag tccggtgcgc ctcatcggct tcggcgccag
     7981 cggactgcgc cgtaccgccg acgccccgca gctcgaccta ttccaaagtc ccggaaaaaa
     8041 gacctccgcc cggcgggaag cgctcagccg cgccgtcgac cgcatccgca gccgcttcgg
     8101 gggaaacagc attcgacgcg gctctaacct gaaacaggaa gacaactaac cactaatctt
     8161 cactgatttt cattttcaac tattagtgct gattagtgag gattagcgga taaactcccg
     8221 tcctaatgaa tccattattt caatcaccgc tgacctgtcc gtcgtgcgaa accgcgctgg
     8281 aatggaacga aaaggaatgc cgctgcgcaa acggccattc gttcgatctc gcgaaagaag
     8341 gttacgccaa cctgctgctc agtcaccagc gccgctccag ccatcccggc gacgacgaca
     8401 agatgattca ggcccgccgg cggttttttg attccggcgc tttcgaaccc gttactgacc
     8461 aaataaaaaa atcggtaatc ggcaatcatc aatcggcaat gtccattctg gactgcggct
     8521 gcggcgaagg tcatttcctc ggcgcgctga gcgaagcgca cgccggacag ttttacggtg
     8581 tcgacgtttc caaagaagcc gtgcgctgcg cggctaagcg gtacaagaaa gcgaaatgga
     8641 tcgtcgccaa tggtatgcgc aaactgccgt tcgccgatca ttcgctcgat gtgattctaa
     8701 gcgttctcgc tccgcgcaat ccggaagaat tcgcccgcat cctgaaaccg gacggcacgc
     8761 tcatcctcgg cgtccccggc ccgaaccatt tgattgaact gcgttcgcgg ctggcggcca
     8821 atgccggtga ctttgaagaa aaggccgatg aggccgccgc caaatgcgcg ccgcacttca
     8881 aggaaaccca ccgcgaactg ctgagttacc agcaaacgct aaacgccgcg cagctcgccg
     8941 acctcatcca gatgacaccg atcttctggt gctccagtcc ggaagcaaag gctgacgtgc
     9001 aacggctcag cgaactcacc gttaccgtca gctttgtgct tctggctttg caaccatcat
     9061 cgtgaaatgt gcttcctttg ttcaagaatt cttctcgcca ttagttgacg gtgctccagc
     9121 tagttcctat cgtcagccac aggaaatgaa tttcgattaa ctttaatact ggccgtaata
     9181 atgcactcac ttccaaaaga tcatccgctt gtaggaacgt ggatcacatc cgacgaagac
     9241 tcagacgtcg ccgtcacaat cgaagtacaa agcggaaaat tcaaagtctc aagcttcaca
     9301 cgtagcgacg gagaatttca caaagtcgaa aatgtaaagt gggctggcaa tgcattgagt
     9361 tttgactcaa cggttccctc taatggatgg caaaccaaac atgtgttccg cattaaacca
     9421 gacgggtctg ccgaagtcga actcacctgg tctgacgtct ggaaaaagaa ggacgtaaaa
     9481 ccaggagaac cgccggaatc gtggcaggca aacagctaga actcgaccag cctcaatgcc
     9541 atttcgggct ggcggtggtc gatctgtctg ccgatagcgg catatttttg aatccgtttt
     9601 tccagcggct gcgccgccag cggattctgc ggatggaaaa gtccgagctt cggattttcg
     9661 gctgccagtt tttcgtgcgc gatcagcgcc cacgccgcat ccactaagtg acgaaactcc
     9721 ggctccagca ggatcgggca gttgaacgac tggcgcgagc tgttgatgtc cacgccgctg
     9781 atttccatca gaccgttggc ctggcagagc ctctgcaaat gaagcagctg cggcctggtg
     9841 ttgcgcggcg gcatgtaggt gatcgctttg aaaccgagct tcttcagctc cggcaccaga
     9901 gcgtcgagat agtcgtcttc aaacttctcg gctttcttgt cgccggtcgg cgattcgcca
     9961 acgtcgccca gataggcgta ggccggaatc gcgccgatgt cgttggcaaa tttcaccgca
    10021 tcgcggacat tgatgcattc gtctttaccg gagagaatga aaaagtccgg aaccagcgcg
    10081 gacttgaaaa cgccgagcag atcataggcg tagtgcggat tattcaaatc cagcaacacg
    10141 gctttcagtt tgccgggaac cggaatctgc atctgcgctt cgacaaacgc gaccagcgct
    10201 gcgccctttc caagcttcgc gatcagcttg agcgacagcg cgtaaagaat gtggcgctcg
    10261 gtaattgatc cgccgttggc cgcttcggaa atcgccatga catctttttc aaaatccagc
    10321 tgcggcgcgc cgagctgcgc cagcttcgcg ttgagccgct cgacttcttt ccggtcgcgg
    10381 gcgatccgcg ccatagtgat cggcttcaaa aactctgtga ccgccgcgaa ctggttgcgg
    10441 ggaattccgt ggaaagcaat atagctgatg ttcggttcgt cggggttgtt ggtttttcgt
    10501 ccttcaacca gcgtgccgtc catgttcaca cggatttcgc agccggcggt agcggcaatg
    10561 ccgatcgctt tggccgcatt cagaaactct tcgcagccgg aaaccgaatc atgatccatg
    10621 attccgaccg tgcccagccc cgcctctttc gccctggcgg gaatttccgc cggggagtaa
    10681 gggctgaacg agtaagtcga atgaacgtga ttgttcacct cgtccgtctg cttcacaccg
    10741 gcttttattg attctcccag cgctttaacc gaagccaacc gttcttcgac tgattttgaa
    10801 tctaagctca tttgttctcc tgtactgcgt attgcgtatc aaaaaatcaa tacgcagtac
    10861 gcaatactcg ttacgttctt acaccccttt acgggcaacg cgcagcattt cgccggcggt
    10921 gtagttgtgt ttcgcaacgt ggcccggcag cggcaggatg cgctgatgga tcgcgtcgat
    10981 cagccagtcg gcctgcggat agtagtccgt ttccatttcg gcggcgggcg agatccagtt
    11041 cttcgcaccg acaaccaccg gcggagcgtc gaggcggtcg aagcagagct gggtgatgtt
    11101 ggcggcgatg ttgtgcagcg cgctgccgcg ttccacggag tccgaagcca gcacgacgcg
    11161 gccggttttc ttcaccgact cgacgatcct gtcgtagttg agcgggttga tgaagcgcag
    11221 gtcgatcagt tcagcttcca gaccgtattg agccttcagc gtttcaacgg cttttttacc
    11281 ggtgtaaagc acggggccga cggtgatgat ggtcaggtct ttgccctgga tgcgtacagc
    11341 cggttcgcct tccggtgttt cgtagtaacc ttccggcacg ccgctcttct caaacatttc
    11401 gccgataccg taggttttct gcgattcgaa gaagataacc ggatcggttc cggccagcgc
    11461 gaggttgagc attcccttgg cgtcgtatgt cgtggcggga tagtaaactt tcaggcccgg
    11521 aatgtggtta accatcgcgg cccagtcctg cgagtgctgc gcgccgtatt tgttgccgac
    11581 ggaaatacga agcaccagcg gcatcttcag gataccggcg gacatcgact gccatttgga
    11641 aatctggttg aagatttcgt cgccggcgcg gcccatgaag tcgcagtaca tcagctcgac
    11701 gcagacgcgg ccgccgctca gcgcataacc gcaggccgag ccgacgatgg cgccttcgga
    11761 aatcggcgag ttgaacaggc gatgatacgg aatcgattcc gtcaggccgt tgtaacaggc
    11821 gaacgctccg ccccagtcgc ggttttcttc accgtaagcg atcatcgtcg gatcggtgta
    11881 gaagcggtgc agcatcgctt cgaaaatacc gtcgcggaac gtgtacagtt tgttggcgcc
    11941 gatcggcttg ccgttttcat cgaacgcata acgggctttg cccgccagct tcttcacgcg
    12001 cggattttct gccatcggca tcagcacatc gggtttgcgg tcatcaaatt tttcgacctt
    12061 gccgttggaa tacatcacat cttcgatgat gttgatgtcg gtgtaaggcg aaacggtttt
    12121 gtcggtcgcc agcctgagcg tcttggtgac cttggcgacg accgtcgctt tcagcgcggc
    12181 ggcttcgtca gcggtcagcg cgccgttgct gatcaggtat tcgccgaaca gcttcacgca
    12241 gtcgttggct tcccacgatt cgatctcctc tttggaacgg taggaggaag cgtcggacgg
    12301 cgagtggccg gagatgcggt aggtgacgac gtcgagcagc gccgggcctt tgccttcgag
    12361 cagaatcttt ttctggcgtt cggtggcgtc ggcgacggcc agcggattgt aaccgtccac
    12421 gcgttcggcg tgcatctgtt ccggattaat cccggctccg atgcgcgcga ccatgccgta
    12481 gcccatcgtt tcgccggcgg tctgtccgcc catgccgtag tggttgttca tgatattgaa
    12541 cagcaccggc ggcgcgccca agctcttgtc ccacagcgtg cggtactggt ccatcgcggc
    12601 catccagaaa ccttcccaga ccgggccgca accgagcgac gcgtcgccga tgttggcaat
    12661 gacgatgccg ggtttacggt tgatcagttt gaacagcgcc gaaccgacgg aaatatcgcc
    12721 ggagccgccg acgagcgcgt tgttcggcat cgagccgaac gggatgaaga acgcgtgcat
    12781 cgaaccgccg agacctttgt tgaagccggt ctgtcgggca aaaatttcag cgagtgttcc
    12841 gtagagcaca aattcttcgg cgagatcttt gacggatttc tgcgagccgt ccttcacgac
    12901 cttgaccgtt gagcctccga aaaactcatc catgatttta gtgagtttcg catcgtcgag
    12961 ctgataaacc gcggaaagac acttggcgag aatttcgccg tgcgagcggt gcgagccgaa
    13021 gatgcagtcg tcggtggtca ggttgaccgc ctgtccgacc gccgcggctt cctgcccgat
    13081 ggagagatgc gccggaccgg ggtggttgta ttcgattccg gcgtacgaac cctgcacctt
    13141 gatctggttg agcatggttt caaattcgcg gacgacgagc atgtcgtagt acatcttctt
    13201 cagacgttcg ccgccgtact tcttcacttc cttcttatag tccggcttgt actgattgac
    13261 cggaatcggc ttgaaggtga tttcgcccgc tttgcggact tcccggggac acacgatctg
    13321 atgcttaggc actgaaacac tcctttgatt taaaactgaa acatggtgtc gcgcacaacc
    13381 tcgccgacgg tcggatgcgg aaaaacaatt tcccggatat ctttcacccg cagctcggct
    13441 tcaatcatca cggcggcacc gtaaatcatt tcggaaacac ctgcgccgac catgtggacg
    13501 ccgagcagca catcggtgcc ggcgtctttg atgactttga cggagccgga accgcgtttg
    13561 ccgttttcgg cgagaaaacg tccgctcatc gtcagcggca gcgaagcagt ttgaaccttg
    13621 tgaccggcgg cctgcgcctg ttcttccgta aggccgcagc cggcgacttc gggcagtgaa
    13681 taaacggccc acggaatcgc gttacggcgg aagcggtcgg gctgtccgca aatcgtgttg
    13741 agcgcgactt cgcccatgcg ggttgcggaa tgcgccagct gggaaaggcc ggtcacatcg
    13801 ccgatagcgt aaacgtccgg caggttggtg cgcatctgtt cgtcaacctt gatgccgcgg
    13861 cggtcgaaat cgagccgggc ttcttcaaat cccataccgg ccagattcgg aacgcggccg
    13921 accgacatca gcacgatatc cgcctgaatg gattgcgact ggcctttggc gtcggtgtac
    13981 ttcacgtcat ggccgtcaat ggccgtcacc ttgcagccga ggttgaaatt tactttgcct
    14041 ttgagcgccc ggcggaattc cttcgcctgc ccgcgatcca tgaacggaat aatttcttcg
    14101 agcatttcaa tcacgtcgac tttaacgccg aggctgctga agaaactggc gaactccacg
    14161 ccgatcacgc cgccgccgat caccgtcagg gtttccggca ttttatcaat gttcaggatt
    14221 tcggtgctgg tcatcacatg cggctgtttg gcgccgggaa tcggcggaac aaacggcgac
    14281 gaaccggtgg cgatgattac attcgtgcct tcgtaaacgg tctcaccgac ctgaaccttg
    14341 cgcgggccga gcagtttcgc cgtgccgttc accacggtga ccttgttttt tttcatcatg
    14401 aacccgatgc cgccgcgcag cgtttcaatc acttcctgct tccaggccat ggctttagtc
    14461 agatcgaact ttacctcgcc ggtcgtcacg ccgaattccg gcgcttcctt ggcgtggaca
    14521 taatgtttag cggagttgag cagcgtcttg gtcggaatgc agccccagtt gaggcaaacg
    14581 ccgccgagat gttctttctc gatcagcaga acttttttac cgagatgtcc ggcgcgttcg
    14641 gccatgacat aaccgcccgg gccggcgccg atgatgataa tgtcgtacat tgttttctcc
    14701 agttcgaccg ataggactta tcggacttat tgtatttcaa aacttaagag cttttccctc
    14761 agtgcagcaa gaaaccgtcc ggcaggcgcg ccgtcggtcg cacagtggtt gaatgtcagc
    14821 gaaagaccca tgtgcggaat gaattcaacg ccgccgtctt tcttcatcac cggcttcggc
    14881 tgaaccgagc agacgcccag aatgccggct tccggagcat taagcaccgg cgtgaacatt
    14941 tcgatacccg acgcaccgag attggtcacg gtgaacgttc cgcctttgag cgcatccgga
    15001 tcgatcgtgc ctttgatgca cgcacggcca agccgcttgg cttccactga aatttcttcg
    15061 agcgaaagtt tattggcaaa gcggatcacc ggaaccatca ggccgcgcgg cgtatcgacc
    15121 gccatgccga ggtggacgtt ttcaaactga accagtttat cgcccagcca gtacgcattg
    15181 aggtcgggga aattgaccag cgtctgcgca acggcataga gaacgaggtc gttgatggtg
    15241 atgcccgctt tatccgcgga cgttttgaat ttcgcacggg cggacagaac cgcgcgggca
    15301 tccgccgacg tgtgcatcgt gagctgcgcc gtagtctgca gagaggaaag catccggccc
    15361 gcgacgactt tgcgcactcc tttgaccgga atttctttca gtccgcccat ctgttcagcg
    15421 ctgtatgaaa ctgccggtgc ggcgggaaca gcagcggatg gcatttgcac atcggcggcg
    15481 agaatgcgac cgcctatgcc gctgccgata gcggcaatct ttgccttggc ggccggtgaa
    15541 agcggagcct gtccggcgag tgcggctttg acatcgcgct caatcacgcg gccttcaggt
    15601 ccggagcctg cgagcgcgga aacatccaga ccttcagccg ccgccagatt gcgcgcacgg
    15661 ggagaaacgc cggatgacaa accggaagac tgaaacttga aacttgaaac tgccgggttt
    15721 gaaacggacg gcttttcttc agctttgctt ccggcttccg gcttcgtttc tgcggcagcc
    15781 gaaggacgca aagcggaaat atcttctccc ggcgcgccga tggcggcgat gattctgagc
    15841 accggaacat ccgcgccgtc cggatagaaa acgccgagta cggttccggc ggcaggagct
    15901 tcgacttcaa aggcggcttt atcagtttcc acttcacaaa gcggctgacc ggccttcacc
    15961 ggatcgcctt ctttcacctt ccagccggta atgatgcagg attcaaccga ttggccctgc
    16021 cgcggcatca aaacttctgt cgccataaat aaagctcctc atccctctgc tttgtaaata
    16081 ctcacgcctg cttcgcgcag ttctttttcc acgtcaacgt ccaggccgga atcggtaatc
    16141 agtccgttca cgccgttgaa gggaagtatc cgtgcaaaac cggctttgtt aaatttggtc
    16201 gaatcacaga tcgcgaaaac ctgctccgcc tgctgcgcca ttttacgcac gagatcggcg
    16261 ctttccacca gatgcgccgt cagcccctgc ttgatcgaaa ctccgtccac gccgatgaag
    16321 gctttggaaa cgaacacctg atccagcgac tgaatcgcca gcggaccgac cagaccttct
    16381 tctgacgggc ggaattcgcc gcctaccagc gtcacgcgca gctgcgggtt ggcgcgggca
    16441 taggtcagca gcagcgtgtt gttggtgaca atgtgcacat tgcgccggcc gagcagatat
    16501 ttggcaatca gcgccgtggt cgtgcctgca gaaataatga cggtatcgcc gtcctgaatt
    16561 ttttccgccg cgaatctggc gatcgccgcc ttgcggtctt tttcctgacg catccgttcg
    16621 agcatctgcg gatgaaacgc cggcatcgcc ccgccgtggg tgcgcacaat gaagccttct
    16681 tcctccagcg ctgcaaggtc ggtgcggatg gtcaccacgc tcacgcccag cttctgggcc
    16741 aggattgaaa cgctggcacg gctgtcttcg gcaagcatgt cgagaatggc ttttttacgg
    16801 tctggaaact gtgctgacaa ttcgctttcc ttctttttat tcatcgaatg cctttctttt
    16861 tacattccat ctgacttctg atcaatatat ttatgctttt tatataatcg cggcaggaac
    16921 cggccctccg ggcatcctgc ccagcacaat acgatcatgc cctgccagat ctttttttac
    16981 ctctgtctgt tcaaacccgg ccttttgcaa aatccggcga acggccggcc cctggtcgta
    17041 cccgatttca agaaacagcc cgccgcccgg ttttaagacg cgggccgcct gctccacaag
    17101 aatccggatc aaatcgaggc cgtctctgcc gccgtcaagc gccagctgcg gttcgaagtc
    17161 gcgcaccgac ggatccagcg ttttacagac atcggaaaga atgtacggcg ggttggaaac
    17221 cgcgatatcg agagagttga cttcgacccc ttcaagcagg ttgttttcaa gagacgaaaa
    17281 ccggtcgatc agtcccagcc gtttcgcatt ttcccgcgcc agcgccagcg ccgccgggga
    17341 aagatcaatc ccgagcactt cggcattcgg aagttctgaa agcagcgcga gcgcaataca
    17401 gccggttccc gttccaatat ctgcaatgat cattgatgac tgccggttgc cgattgaaga
    17461 aagaaccgca tcaacaagga gttctgtctc ggggcgcgga attaaagcgc ggcggtcgca
    17521 ccggatttcg agaccccgga aatcaacgtg gccgatgatg tactggatcg gctcgctctg
    17581 ttccgcgcgg acaatcagct tttcgagttc agcctcctgc tccggcgtga ttttccgaaa
    17641 atagatttcc agcggcttac agccgagaac gtgcgccgcc agctcttcaa ccgaacgttt
    17701 ggcattcccg ctgccggccg ctgccagccg catttcatat tgtctgatca aggcattcat
    17761 tattggctgc aaactgtagg ccgggcgccc gcgccgggcg agaaattaac aaaccatttc
    17821 cggtttgccg cccccaatcc attaggctac tttcgcagac gcagaaatat gggtctagcc
    17881 tgcaaacagc tgaagcgagg ttaatacacc atacgacaga gaagatgatc gatatcaata
    17941 cattcgagga agtccgccag cgattcgggc actttgcgag ctgggctgtt tgggcagagt
    18001 caggcggaag tctgaaagac aatatcgcag accttactgt tttcgacccg gaatccaatc
    18061 cccaccttct ccaaacgctt cacgtcaacg ccattcttat cggactcaac atctcgcgac
    18121 caattgaacg accacttggg aactttcatg acccacgacc aatggccaca gactttaaaa
    18181 tccgatatgc tttgtacgga acgccctatt ggggagccta tatgactgac ataattaagg
    18241 acttcgaaga aaaggtttcc ggaaaaatgg tgggatttct aaaagcccac cctgatttcg
    18301 aaagagataa tatcaaacaa ctgaaagctg aaatcgacgt tctagggtgc tcaaatccag
    18361 ttctgattgc ttttggaaaa gatgccgaaa ggatagccag acgaaatctt ggcgacgagt
    18421 attccatcgt gggaattccg cattatgcga attatatatc gaaggaggac tatcgaaacc
    18481 aggtgtggga tgttctgcag tgccaaggta gaaaataatt caaaactgta ggccgggcgc
    18541 ccgcgccggg cgagaaatta acaaaccatt tccggtttgc cgcccctgaa ccgccaagct
    18601 acattcatgg acgcattaat ttaatccgca aaaaaactga agtacggctg atgttgattt
    18661 taaaaaggaa ctgcttatga aaaacggaca gcgaattaca gtcttcatgc tgactttact
    18721 gttgagctgc ggcctgtccg cccaagacaa ttccagtccg caagactggc agatcccggc
    18781 gcaaaaagag aacttccaca ttttcctgct gatgggccaa tccaacatgg ccggatttgg
    18841 cgagatgctt ccggaggaca cacagccggt tccacgggtt ctcaagctgc ccaccatcgg
    18901 cgaggttcaa tgggaaccgg ccgcccaccc gctgcacaac cggcagaagt ccgaccgttt
    18961 cgggctcggc ctgccttttg cagaggaata cctcaaagac aaaccgggtg tcgtggtcgg
    19021 actgattccg gtcgcccagg gcggcgccgc catcgacaag atgaacaagg gcaccccgat
    19081 ctatgccgac gccatcaaga aagccgaatt cgccgccacg cagggcgtca ttaaaggcgt
    19141 cttgtggcac cagggcgaat ccgacaccac gtccgccccc cgcaccgata tatacgaaca
    19201 gaagctccac caactcattg ccgatttgcg gcaggacttg aaggacgaca acctgccgtt
    19261 cattgcagga aatctggccg agttctacgg cacaggcccc gagcacaaca agcccgaccg
    19321 cgtggcgaga atagatatgg ttcgcgccac gttgcgcgca ctgccggaca aggtcaaaaa
    19381 taccggcttt gcggaatcaa ccggctgctc gtcggcagac gcgcacatgg ttcattttga
    19441 caggaactcc tacatcattc ttggaaagcg gtatgcggag gtctattcca ggatcattaa
    19501 caaagagctg aagacgccgg gtcaacttgc gccttagttc aactgttcgt cggctggttt
    19561 tccgacggct gccctctgtc cgccggtgct acgcgccgat gccgtacttc tgacactctt
    19621 cgagagcgta gcggtcggtg cagccggcaa cgtagtcgca gacggtgcgc cggagtcctt
    19681 cttcggcaac acgcgccagc gccttgcggc ccatggtttc cggatgctca atgtagtgca
    19741 gaaaaagacc gcgcatcagc gcgaccgctt catggttggc attcagaacg gccggatgct
    19801 cgtagagctg ccggtacatg aactttgaaa actcatcgag cagtccgccc atttcatcgc
    19861 cgaatgcaac aagccgttca ggcgcattca tgacatcggc gatggcgcgg ggattgtgcc
    19921 tttcaatccg gcgccgccct tcacgcagaa catcctgcgc ctgtaaatcg agcaggttgc
    19981 ggatagtgat acgcagaaag tgtccgtcat cgagcgcggc atactgctca cgggtgcggg
    20041 cggcggccag cttccagaaa gccagctttt cgagctgttc cagcgtaatc agtcccgctt
    20101 cgagaccgtc gtccacatcg tgcgcgtgat aggtaacgtc gtcggcgaca tcggcaatct
    20161 gcgcttccag atactggaag gggccgatcg gatggccgtc gagctgcccg ccgggtttgg
    20221 ccgcctgatg tttgagcagt ccggcgcgca cttcccatgt caggttgagt ccgggaaagc
    20281 cgggatactg gtattcgagc ttctcaacgg cgcgcaggct ctgaagatta tgatcgaatc
    20341 cgccgtgatc tttcatcagt tcatcaagaa catattcgcc gcggtggccg aacggactgt
    20401 ggccgacgtc gtgcgcgagg cagatggttt cagtcaggtc ttcgttgacg cgaaacgcgc
    20461 gcgccagcgt ccgcccgacg gcggccattt cgatggtatg ggtgagccgc gtccggtagt
    20521 ggtcggcggt cccgttgaca aaaacctgtg ttttatattc cagccgccgg aagcaccggc
    20581 tgtgcagtac gcggtcgcgg tcgcgctgaa aacaggggcg caccggatgg tcgtcttccg
    20641 gataaaggcg gcccaggctg cgggcgctgt gcgcggcgta cggcgcgagg tcgcgcgatt
    20701 ccagtttttc ccattcctgt cgggcgtgtg tcatggcggc agtttagcgg attgagctct
    20761 tttgacaaat agtactgcgt atttcgtatt acgtattttg tattgtctgg attttgggca
    20821 acagctcccg gtacgcaata cgaaatacgc aatagtcgtt cctttacccg accaggattc
    20881 cgcagtacgt tttccgccat gcatgagaag accatcggat caaaaacagt ttttaaagga
    20941 cgcatcctga gcgtggatgt tctggatgtg gaattggcgg acggacgaac cggtacccgt
    21001 gaaattgtcc gccacggcgc ggcggtcgcg gtgatcgccc ggcggcgcga cggccggttt
    21061 gttttcatcc ggcagttccg taaagcgatg gaacgcgtct gtttcgaggt gatggccggc
    21121 aactgcgatc cggacgaagc ggcagaagcg gcggcggttc gcgaactcaa agaagaaacc
    21181 ggttacgcgc cggattccat ccggtttctt tcttccatct acccgtcggt cggctactgc
    21241 accgaacgaa tcgatatttt ttacgcggaa gtcagcgaac cgggcgaaac tgattttgat
    21301 cacgacgaaa acattgagac cgtcctgctt accaaagatg aaatggatgc catgatccgt
    21361 gccggcagtg tgcaggacgc caaaacactc gccgcctgga tgctttatac ggtttatatg
    21421 taatgcaaat ccttacaaca tcgtcaggat taagttttcg acatctgtct aaatacggat
    21481 aaactgtcgg cacttcacaa ggag
//
LOCUS       DAOPZO010000017        21299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_2535_length_21299_cov_8.288882, whole genome shotgun sequence.
ACCESSION   DAOPZO010000017 DAOPZO010000000
VERSION     DAOPZO010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 21299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21299
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2535_length_21299_cov_8.288882"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PKI68_02320"
     CDS             complement(<1..371)
                     /locus_tag="PKI68_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52559.1"
                     /translation="MKIAAAAVTFSLLTLIGYSTPNPDPKFSATASIDITKRTVDSEH
                     NARPPLDAEVEHVVSRPILDEVARQLHLAEKWTENGIEPSSDKIYSRIKNMLTVRPVA
                     VISLIRITAKSDDSAEAVAVA"
     gene            complement(799..1695)
                     /locus_tag="PKI68_02325"
     CDS             complement(799..1695)
                     /locus_tag="PKI68_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNX52560.1"
                     /translation="MDDLLIKGHFKGLDIAFTYAVTTKAVNEIVLRHNCDPAAAHILG
                     RAVTGALLAAAILPEGRRLNACWKYKGILRTIVADAGQDGTVRGFISPTQLNLEDDDP
                     DKLYGDIGDLQVVTSHAGKILNSGTAPVSLHDVAGDLAYYFCISDQVETGLSAMIGFN
                     ADPENPVRLCQGWMIQALPGTDLEHFDRVRQRMDGFSFRELLSRESETDGYFETIANS
                     LIDGDAGFTGLHMETCPAPRFACPCSHEKMEAVVRTLPIPDRMELVQKKEPVAIHCRF
                     CNRRYELSIDECIAAWNKKESH"
     gene            1781..2302
                     /gene="rnhA"
                     /locus_tag="PKI68_02330"
     CDS             1781..2302
                     /gene="rnhA"
                     /locus_tag="PKI68_02330"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF001236.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNX52561.1"
                     /translation="MDDAVEIYTDGACKGNPGPGGYGVVLLAGGRRKELSGGFRKTTN
                     NRMELLACIEGLRSLKRPCTVVLTSDSKYVVEAMNKSWAKRWRANGWKLSPSKPAKNS
                     DLWKQLLDLCGSHQVSFKWVQGHNGHPENECCDVLAVAASQLKDLPADIAFETGVLAD
                     DLFSGTVPASSVI"
     gene            2342..3850
                     /gene="malQ"
                     /locus_tag="PKI68_02335"
     CDS             2342..3850
                     /gene="malQ"
                     /locus_tag="PKI68_02335"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNX52562.1"
                     /translation="MNFERKSGILLHITSLPGRWGMGELGPQARAFGKFLAVAGQKLW
                     QILPTGPVGYGYSPYSSLSAFAGNHLLISFDELIEEGRLTEKRLAKFPQFDPHRIDFP
                     AVIEAREKFLFVAADRFGPDADFAEFCEREADWLSDYALFMAIRETQSNRPWTEWPDG
                     LRDRDSHSLEKARKRLAKRIRRHEVLQYLFFRHWKKTTEALRPSGISMIGDIPIFVAG
                     DSADVWANRELFFLNGDGTPSVVAGVPPDYFSATGQRWGNPLYNWEVHRETGYAWWTK
                     RMRRILEMTDIVRIDHFRGFEDYWEIPADEPTAVKGRWVKGPGLELFQALEKNLRHDP
                     VYGKNFRLDEHVIAENLGIITEEVEALREACGFPGMWVLQFRFGAEDMNRAGYHPEGT
                     EKNWTVYTGTHDNDTTAGWLHSLDKETHQRVCNWLQAGSSVSHHELAELALRAPAKWA
                     VLPMQDVLGLGGAARMNMPGTTQGNWGWRFSEDMVTTATVQWLREMTERNRR"
     gene            3938..5017
                     /locus_tag="PKI68_02340"
     CDS             3938..5017
                     /locus_tag="PKI68_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNX52563.1"
                     /translation="MDSATVTTVVQQAIEKNTFFDFLLMILLVLTGFLAGRFLKYFCE
                     QAIRKTPLGQNSLHRTALELAARTTTFPASVIGVCFALKVLAIRGALRGDVIAVLVTV
                     VVTYIVYQAVELSGWWVRHLRAKTRTALDDMMEPVVRKTLRTIVVILGLVQVAQQLSD
                     KPITSILAGLGVGGLAVALAAQDTIKNFFGSLVIFADRPFQLGDSVCVDGIDGSVEEV
                     GMRSTRLRTADGHLVTIPNGELANKTIRNVTRRPYLKRVTNLALACDTPSGKVDQAIA
                     IVKEELNRANEKLHPGMPPRVHFNEFNAASLNIQVIYWFAPASDYWGFMEFDQKFNLA
                     VLRRFNEEGIEFAFPGRIQDPGKTG"
     gene            complement(5014..7113)
                     /locus_tag="PKI68_02345"
     CDS             complement(5014..7113)
                     /locus_tag="PKI68_02345"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HNX52564.1"
                     /translation="MRKHPLYLLLLAAVLAGCGKPEVPANAANALAQDPIPSERIAAR
                     IADRLPHIHLNRNAFDDSIATNALALYIDSLDYDHTFFLASDIAEFQKQATNLDNQVV
                     AGDTSYAQVIFDRFKERVTNRVAYVNQLLNQPMDLDQAEKYRWKRDTAPWPENETEWN
                     DLWRKKVKNDYVSRIAAIEAGKNDPKPEGTNTVDEARIEALTPVEFVRERYKQYQLLI
                     DNNFDKETVLQRYLSAFTRSYDPHSEYLSPRGVEDFDINMSLSLVGIGAMLRTEDGMA
                     KITQLIAGGPAELDGRLRAGDKIIAVAQSNAAPVSILYWPLSKAVRLIRGEKGTKVVL
                     TIIPADDTTGTRTKTIDLIRDEVKLEAQAAKGEVRQITETNGVPHRIGVLTLPEFYAD
                     FAAARNGKDGARRASVDVRRILGEFAAKRVDGVILDLRNDGGGSLAEAIDIAGLFISA
                     GPVVQVREQRGVAVLPDGDPDTVYSGPLVVLVNRLSASASEIVAAALQDYGRALIVGD
                     SKTHGKGTVQTVFPLSKLSDDLGSIKVTTASFYRIAGGSTQLKGVVPDVVLPSLYDAL
                     DIGEESLPKALPWSQVRSAYYRMWPDSVKPAIPELRKHSEMRMAGNPAFTTFLARRDR
                     LRSRMANPEISLKLSDRVGEIVAEKELEKLQDEELGGSKKADKEDPILDETVNILADM
                     VDLAGEKSGSVVTQNRN"
     gene            7241..8848
                     /gene="pgi"
                     /locus_tag="PKI68_02350"
     CDS             7241..8848
                     /gene="pgi"
                     /locus_tag="PKI68_02350"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNX52565.1"
                     /translation="MLTKRPEWKALSAHWKKTQSAHLRDLFAADPQRAEKFSMSACDL
                     LLDYSKNRITDETMSLLVQLAEASGLRGKIEAMFSGEKINRTEDRAVLHTALRAPRGT
                     KVCVDGEDIMPGIHAVLDQMEKFSNSIRSGEWKGYTGRPIRNVINIGIGGSDLGPVMA
                     FEALKPYSQRNLTIRFVSNVDGTHIAEAVRDLNAEETLFIVASKTFTTQETMTNAETA
                     RTWLLAALKDKAAVAKHFVALSTSAAEVSKFGIDTANMFGFWDWVGGRYSLCSAIGLP
                     VMIAIGPQNFRKMLAGFHAMDCHFRTAPFAENMPVILALLGIWYNNFFSAETQAILPY
                     DQYLSRFAAYFQQGDMESNGKYITQDGKRVKWQTGPVIWGEPGTNGQHAFYQLIHQGT
                     KLIPCDFIGFAKSQNPLGDHHAKLMANFFAQTQALAFGKTAEELKAEKTPKKLIPHKT
                     FEGNRPTNTIMAEKLTPEVLGHLIALYEQKIFVQGMIWDIFSFDQWGVQLGKVLASAI
                     LPELESRSELKHDASTNRLIEYFRKNK"
     gene            9016..9402
                     /gene="crcB"
                     /locus_tag="PKI68_02355"
     CDS             9016..9402
                     /gene="crcB"
                     /locus_tag="PKI68_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNX52566.1"
                     /translation="MKILLFIGIGGFLGSIARYGMTLGVQHWAGGGRLGDFPVGTLAV
                     NLLGCLVLGFLGHLLSERFEMAAEIRFALTVGFIGAFTTFSTYAYETLAMAREGEQLF
                     AWLNILASNALGLFAVWGGYRLGGLV"
     gene            9533..11995
                     /locus_tag="PKI68_02360"
     CDS             9533..11995
                     /locus_tag="PKI68_02360"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNX52567.1"
                     /translation="MENFTPRAQRVLHLARKEAEQFNHNYVGTEHILLGLVALGSGVA
                     VSALQSLGVDLQSLRLEVEKAVGTGSDTKMTGNIPFTPRAKKVLALATSEARSLNHSY
                     VGTEHILLGLLREGEGIAARVLENMGVDLEEAREEIMAMLDPDYDGSMESANEEEAPA
                     FGAPQKTAEGQAVKTPALNAFGRDLTEMAKKGELDPVIGRKNEIERVIQILCRRTKNN
                     PVLLGEAGVGKTAIAEGLAQSIADGTVPQLLADKKVVTLDLALMVAGTKYRGQFEERI
                     KAVMDEIRKTKNVLLFLDEMHTIVGAGSAEGTMDAANIIKPALSRGELQCIGATTLSE
                     YRKSIEKDAALERRFQTVIVKEPTIEETIQILKGLRPKYEEHHHTKISDAALESAAKL
                     SARYLPDRFLPDKAIDLIDEAGARSHIANMVKPPEFQEIRQRMMEAQQKKDTAIREQK
                     FEEAASMRDAERKAKEEFDAAMKAWEASRQESLSTVDEEEIRIVVSKWTGVPVDKMGE
                     QALAKLLQMEAQIEKSVIGQNEAVSAISRALRRSRAELKDPRRPIGSFAFLGPTGVGK
                     TMLAKELARFMFDDPDALIQIDMSEYMEKFSASRLVGSPPGYVGYEEGGQLTERVRRR
                     PYSVVLFDEVEKAHPDVMHMMLQILEEGRLTDSLGRSVDFRNTIVIMTSNVGATHSAK
                     AGLGFSPISEQNDYDKLKGVMMAACKETFKPELVNRLDDIIVFRQLNEKDIEIILDLE
                     IARVRERVAARKITLELSAEARQFLIKKGFDKAYGARQLRRAVEHQLEDPLAEAILRG
                     EVVGDKPVGVGVSGEKLAFTQV"
     gene            complement(12016..13917)
                     /locus_tag="PKI68_02365"
     CDS             complement(12016..13917)
                     /locus_tag="PKI68_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX52568.1"
                     /translation="MAFKLGLTIFLIAALSFTGLGIYYTRLFSQQIDKQLYTQARIPG
                     RLMNQQVLPYSAVRDRGALSDLIGENVIYASVNRRDHCIHYCTDPAREGTYNTCLESA
                     GTVQNAAGETLSILAQDNAVYLHITTPLFANNQRLGELHIKIDTGRTAAKKKAVASTF
                     FSGGLFCVLLITFAGVILIRKLTIPRIKTAGQCLKAVSGGNYNARISHADSPDELGLL
                     ERGINNMIAQLKERHQEDTRLRNELQTAKENAEAANRSKSEFLANMSHEIRTPMNGII
                     GMTQLMEDTELTAEQADYIQTIFTSAEKLMAIINSILDLSRVELGKFELKNEPVCVPD
                     LLMELHKFFTPAVHSKGLDLHIDGIKDVPRVIRADESCLRQVLINLMANAIKFTHKGH
                     VRVSVRCPRKTGTDCTLEFSIQDTGIGITKEAQKIIFREFTQADGSHTRKYGGTGLGL
                     AISRRIVEKMGGSLTVTSEPDHGAVFSFSITVPLDQQTEQTVSSEAARPPAYALTRAP
                     SILLVEDNKLNQRVIMKMLEKEGCLIDLAEDGQDAVEKLRLTEPPEQRPAYDIIFMDI
                     QMPVMDGLQATAAIRKHDKTVPIVALTAHAMKGDREKFIEAGMSDYLAKPIHREEMQA
                     ILNLYTPRT"
     gene            complement(13935..15236)
                     /locus_tag="PKI68_02370"
     CDS             complement(13935..15236)
                     /locus_tag="PKI68_02370"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNX52569.1"
                     /translation="MQKHLKFILISLLCFYVLPVFAQDGPVQQLRWMGHWEGEGLREQ
                     LVREVLDDFTFENQNIRVQFKFSADVLQEKSQSAEAAFLADMIRSGNITWDVVWMDAL
                     IYRKVAVLLNDPDWGRKYLVDFAEVPGFKETQKPFLVDGPDCHKQTGGVFTGPYIEGF
                     FYALWYNTEVAAALGLQIREEEMSADDLLSYARRVDEYNRTADVPVSLFADNYNSGAF
                     GRLAYNLFNSAAPEDSAGNTEPAIRQVLDTFGKLGRLHPVLYSEPKGTWLDTARRLTE
                     NKALFMIEPTWRYNTLKDSFPQSLRKLRLAQMPGFQKQRYYAGGFIPVWAVMKNSPNR
                     AAAIRLMQFWSRPEIAEKWIRYTKSPTGLAGNLYDPEYGNDLFAEYQRKLALNRTLKP
                     DIFISESDHSPITPVFRSLKPLLRAEISAGEAYRKFEEKSE"
     gene            15415..16251
                     /locus_tag="PKI68_02375"
     CDS             15415..16251
                     /locus_tag="PKI68_02375"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNX52570.1"
                     /translation="METIAILGTGLMGSSLGLALRKRNVPVRIHVYARRPETRETALN
                     FGVADAVFSDPVEAVKGAGLIVLCVPVLTIPELAKACRAGLRPGAIITDVGSTKACLN
                     ELMTEVLAGTGAEFIGSHPICGSEQQGIEAGNADLYDGAVTVVTPPMLADDVSVEKVS
                     SLWKSAGARVTIMDAASHDRILAATSHLPHVVAAALALSSGDNGLFCGSGFRDTTRIA
                     DGSPQVWSDIVRTNAPALKAALKIFCDNLNELAALIDEDNGEKLANWFAAARDKRKEL
                     LR"
     gene            16251..17558
                     /gene="aroA"
                     /locus_tag="PKI68_02380"
     CDS             16251..17558
                     /gene="aroA"
                     /locus_tag="PKI68_02380"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNX52571.1"
                     /translation="MNSKTVYPVSKFGGELRVPGDKSVSQRIAMLAALAKGTSEVRGF
                     LHGEDAMSTLSAMCSLGASAKFEGDVLKITGTGGKFMEPANPLDMGNSGTGTRLLAGL
                     LAGQKMTVTMTGDASLSRRPMGRIKTPLELMGAKLDLTGEKGTLPLTIHGTPLHGICY
                     KLPMASAQVKSCVLLAGLFAEGKTTVIEPRPTRDHTEKLFQLLEIPLHIDGLEITLQG
                     FGPKGPQIAARNLTVPGDFSSAAFWIAAVAARPGATLTVRGVGLNPRRTALLDVLKRM
                     GALIEVEVAEEHGDPIGTVFVRGAQLRGTEIGGDEIPNLIDELPMLAVTGALAKGKTV
                     IRDAAELRVKESDRIAVTAAHLRAFGVHVDEHPDGMTVHGPAMLHSPDNPLASHGDHR
                     ITMSMAMLATFSDAPVKLEQVECVATSYPDFWNHLEQLGGKVE"
     gene            17555..18892
                     /gene="cmk"
                     /locus_tag="PKI68_02385"
     CDS             17555..18892
                     /gene="cmk"
                     /locus_tag="PKI68_02385"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNX52572.1"
                     /translation="MNRQRIIAIDGPAASGKSTVAREVAKRMNCIYVDSGSLYRGVTW
                     KMLQEGVCTRSPLLVLPVLLKSKWKFEVRNSAVVFTIDGVEPGEALRGKEVREAVSDI
                     AAMPAVRTFVVNRLRKLRKLGSLAMEGRDIGSVVFPKTPYKFYLDANPEERAKRRHAE
                     LLASGETEKAQEVLESLQRRDQKDSSRKTAPLKIATGAYVIDTSGYDIEGVIRILMDR
                     IAELEKPRSDKMINPIWYRITCKVFGICLTVCNRYKVFGIKNVPKTGGVIIASNHASY
                     LDPPAVGASSRKMRMTHFMARDTLFRNPLMGAFLRRVGVIPLDRDRGDVKAMKTAIQL
                     LRDGASVALFPEGTRSPDGTLQPAKPGVGFLVAKGNAPVVPVYVHGSYEAWSKNSGGL
                     KCKPVTVIFGRLITQEEIRGLGEGRDAYGLIGELIMKRIAELKENFEKGLISE"
     gene            18960..20465
                     /gene="proS"
                     /locus_tag="PKI68_02390"
     CDS             18960..20465
                     /gene="proS"
                     /locus_tag="PKI68_02390"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNX52573.1"
                     /translation="MSKQAKTAISPTREENYPEWYQQVVRAAELAENSDVRGCMVIKP
                     WGYALWENIRTALDGYFKATGHVNAYFPLFIPLSYLEKEAEHVEGFAKECAVVTHHRL
                     EAGPNGKLIPAGELEEPLVVRPTSETIIGATYAKWVQSYRDLPILINQWANVVRWEMR
                     TRLFLRTAEFLWQEGHTVHETSAEAWEETVKMLNVYKQFAEEYMAMPVLIGEKTAGER
                     FPGAVNTLCIEAMMQDCKALQAGTSHFLGQNFARASGIKFQSREGREEIAWTTSWGVS
                     TRLIGGMIMTHADDDGMVMPPRLAPSHVVILPIIRKEEEREKIMQYCNETAAMLRAQT
                     YAGRPVDVIVDGRDINAGEKGWSWVKKGIPLRVEIGPRDMENGAVYVARRDKSAKEKY
                     GQNRAEFVAAVTDVLADMQKNLFDRALAFRAQNTKEIDSWDEFVTFFKEGRGGFALAH
                     WDGTEETEQKINDELSVTIRCIPFDCEQSGAGKCVVSGRPSKGRVVFAKSY"
     gene            20486..21076
                     /locus_tag="PKI68_02395"
     CDS             20486..21076
                     /locus_tag="PKI68_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52574.1"
                     /translation="MAKGRFKIQGTKDFLVAAVFCGFLCIWAIRDAWFPTEKVLKKHP
                     HEIPVAFTVSGVVKDVQVKAGDEVSGNIVLASLYDEGYRAKVTEAEAAFEAAKTAKDP
                     AVEKKLDVLMKARADAEACSLQTANIKWTTTHGEEVLRGIVARILVEPATRIEAGTPI
                     LTVQPADTFYLFNKTLALLSFIGMIVALVFHGIASR"
BASE COUNT         4667 a         5552 c         6316 g         4764 t
ORIGIN      
        1 ttggcaacgg ccaccgcttc ggcagaatcg tcactcttgg cggtgatgcg aatcagtgaa
       61 attactgcga ccggtctcac cgtcagcata ttttttattc gggaatagat tttgtcggaa
      121 gaaggttcaa tcccgttttc tgtccatttt tcggcaagat gcagctgcct cgccacttcg
      181 tctagaatcg gtcgggagac gacgtgctcc acttcagcgt ccagcggggg gcgtgcgttg
      241 tgttcggaat ccacagtccg cttcgtaata tctatggacg cggtggcaga gaatttcgga
      301 tcaggatttg gcgtggaata accgattaga gttagaaggg aaaaagttac ggccgccgct
      361 gcaattttca tatttccatc caatggtggg gtgtgaagtt gggcgaacct taatgcgcgg
      421 cgaacacttg gtcaaattgg aacaggtttt aatcgccgag tcggccatgc aaccgcatgg
      481 aggatttcgt tctgtcgaaa ccgggttcgg cggcgacgga gcgccgccct ccattggaat
      541 acttccggca ctgtttacgt cttcgctttt caggaaaggt cgtaatcgtc ttaaggcagg
      601 cgggacgcct gcgatacgta ccgccggcat cctgccggcc ttgtcccgaa caaactgacg
      661 ccggtattac cgctgtccgc caaaggaaga aacgcgcacg gagttcgcgt ttcgcctgcc
      721 gcgcccggcg gacgcggctg cagcggtggg tgcggacgct gccgcgcctg agtcggacta
      781 taggctaaag cctatggcct aatgactttc ctttttattc caggcggcaa tgcattcgtc
      841 gatcgagagt tcatagcgcc ggttgcagaa ccggcagtga atcgcgacgg gttctttttt
      901 ctgtaccagc tccatgcggt cgggaatcgg cagggtgcgg acaaccgcct ccattttttc
      961 gtgactgcac ggacaggcaa aacgcggagc aggacaggtt tccatgtgca gaccggtaaa
     1021 cccggcatct ccgtcaatca gtgaattggc aatcgtttca aaatagccgt cggtttcgct
     1081 ttcccggctg agcagttcgc ggaaagaaaa accgtccatc cgctggcgga cgcggtcgaa
     1141 gtgttcaaga tcggttcccg gcagcgcctg aatcatccat ccctgacaaa gccggacagg
     1201 attttccgga tcggcattga agccgatcat ggcgctgagg ccggtctcaa cctgatcgct
     1261 gatgcagaag tagtaggcca gatcgccggc aacgtcatgc agggaaaccg gcgccgttcc
     1321 ggagttaaga attttaccgg catgcgaagt gacgacctgc agatcgccga tgtcgccgta
     1381 cagcttgtcc gggtcgtcat cttcgagatt cagctgcgtt ggcgaaatga acccgcggac
     1441 ggttccgtcc tgaccggcat ccgctacgat ggtgcgcagg atgcctttat atttccagca
     1501 ggcattcagg cgccggcctt cggggagaat cgccgccgcc agcagcgcgc cggtgacggc
     1561 gcggcccaga atatgagcgg cggccgggtc gcaattgtgc cggagaacga tttcgttcac
     1621 cgctttcgtc gtcaccgcat aggtgaatgc aatgtcgaga cccttaaaat gtcctttgat
     1681 cagcagatcg tccacggtag gttccttttt ttgaaagccg gaagggtagg gaaccgggcc
     1741 cgactcggca agctcctgtt tcacagattg gaaaattctt atggatgacg cagttgaaat
     1801 ttataccgat ggtgcatgca aggggaatcc cgggccgggc ggctacggcg tggtgcttct
     1861 ggccggcggc cggaggaaag agctttccgg cggattccgt aaaaccacca acaaccggat
     1921 ggaactgctg gcctgtatcg aaggactgcg ctcgctgaag cgtccctgca ccgtggtgct
     1981 gaccagcgat tcaaaatatg tggtggaagc catgaataaa agctgggcca aacggtggcg
     2041 agccaacggc tggaaacttt ctccgtccaa accggcaaaa aattccgatc tttggaaaca
     2101 gctccttgat ctttgcggca gccaccaggt cagctttaaa tgggtgcagg ggcacaacgg
     2161 ccatcccgaa aatgaatgct gcgatgtgct ggcggtggcg gcgtcgcagt taaaggatct
     2221 gcctgccgac attgcctttg agaccggtgt cctcgccgac gatcttttca gtggaacggt
     2281 cccggccagt tccgtcattt gatgatttga ctccccgccg gttctccgta tcctgccgcg
     2341 aatgaatttt gaacgtaaaa gcggaatttt actccatatc acttcgttgc cggggcgctg
     2401 ggggatgggc gagctcggtc cgcaggcgcg ggctttcggg aaattcctgg ccgtcgccgg
     2461 gcagaagctc tggcagattc tgccgaccgg cccggtcggc tacggctatt cgccgtactc
     2521 atcgctttcc gcctttgccg gaaaccatct gctgatcagt tttgatgagc tcatcgaaga
     2581 agggcggctg acggaaaaac ggttggcaaa attcccgcag ttcgatccgc accgaatcga
     2641 ttttcctgcc gtgatcgagg cccgtgaaaa atttctgttc gttgccgccg accggttcgg
     2701 gccggatgcg gattttgcag agttctgcga gcgtgaagcg gactggcttt ccgactacgc
     2761 gctgtttatg gcgatccgcg aaacacaaag caaccgtccg tggacggaat ggcccgacgg
     2821 tttgcgcgac cgcgattccc attctttgga aaaagcgcgg aagcggctgg cgaaacggat
     2881 tcgccgccac gaagttcttc aatatctgtt tttcaggcac tggaaaaaga cgacggaagc
     2941 tcttcggccg tctgggattt cgatgattgg cgacattccg attttcgtgg ccggagattc
     3001 cgcggatgtc tgggcgaacc gtgagctgtt ttttctgaac ggtgacggta cgccgtcggt
     3061 cgttgcgggc gttcctccgg attatttttc cgcaaccggc cagcgctggg gcaatccgct
     3121 gtacaactgg gaggtgcacc gcgaaaccgg ttatgcgtgg tggacaaagc ggatgcgccg
     3181 catccttgaa atgacggata ttgtgcgcat cgaccatttc cgcgggtttg aagattactg
     3241 ggaaattccg gcggacgagc cgacggctgt caaaggccgc tgggtgaaag gtcccggcct
     3301 cgaactgttc caggctcttg aaaaaaatct ccgccatgat ccggtttatg gaaaaaattt
     3361 ccggctggac gagcatgtca ttgccgaaaa tctcggcatt attaccgagg aagttgaagc
     3421 gttgcgcgag gcgtgcgggt tccccggcat gtgggttctg caattccgtt tcggcgcgga
     3481 ggatatgaac cgtgcgggct accatccgga aggaaccgag aaaaactgga cggtttatac
     3541 cggtacgcac gataacgata cgacggcggg ctggcttcac tcgctggata aagaaacaca
     3601 tcagcgcgtc tgcaactggt tgcaggccgg cagctctgtg tcgcatcacg agctggcgga
     3661 gctggcgttg cgggccccgg ctaaatgggc ggttctgccg atgcaggatg tgctgggact
     3721 tggcggcgcg gcgaggatga atatgccggg cacgacgcag gggaattggg gctggcggtt
     3781 ttcggaggat atggtaacca cggcgaccgt tcagtggttg cgggagatga cggagagaaa
     3841 caggcggtag agatattatt gcgtactccg tatttttacc ggattgcaac ccggcaatac
     3901 gcaatacgga ataagcggta cttgtaagga gttttttatg gattcggcga cggttacaac
     3961 agttgtgcag caggctatag agaaaaatac cttttttgat tttctgctga tgattcttct
     4021 cgtgctgacc ggattcctgg ccgggcggtt tctgaaatat ttctgcgaac aggccatccg
     4081 gaaaactccg ctcgggcaga acagtttgca ccggacggcg ctggaactgg cggcgcgtac
     4141 gacaacgttt ccggcgtcgg taatcggcgt ctgttttgcg ctgaaggtgc tggcaatcag
     4201 aggtgcgctg cgcggtgatg tcatcgcggt gctggtcaca gtcgtggtga cctatattgt
     4261 ttatcaggcc gttgaacttt ccggctggtg ggttcgccat ttgcgggcaa aaacacggac
     4321 ggcgctggac gacatgatgg agccggtggt tcgcaaaaca ctgcgcacga ttgttgtcat
     4381 tctcggtctg gtgcaggttg cccagcagct gagcgataaa ccgatcactt ccattctcgc
     4441 cggactcggc gtcggcggtc tggccgttgc gctggcggca caggatacga ttaaaaactt
     4501 tttcggctcg ctggtgattt ttgcggatcg tccgtttcag ctgggcgatt cggtctgcgt
     4561 agacggcatc gatggttcgg tggaggaagt cggtatgcgg tcgacccgtc tacgaacagc
     4621 cgacggccat ctggtgacga ttcccaacgg cgaactggcg aataaaacca ttcgcaatgt
     4681 gacccgtcgt ccgtacctta agcgcgttac caatcttgcg cttgcctgcg acacgccgtc
     4741 cgggaaagtg gatcaggcga tagcgattgt caaagaggag ctgaaccgcg cgaacgaaaa
     4801 gctgcatccc ggcatgccgc cgcgcgttca tttcaacgaa ttcaacgccg cttcgctgaa
     4861 cattcaggtg atctactggt ttgccccggc ttccgactat tggggtttca tggagtttga
     4921 tcagaaattc aatctggccg tgctccgccg gttcaatgaa gagggaattg aattcgcgtt
     4981 tccgggccgg attcaggatc ccgggaaaac cggttagttc ctgttttggg taacgacgga
     5041 accggatttt tctccggcca gatcaaccat atcggcgaga atattcaccg tttcgtcgag
     5101 gatcggatct tccttatcgg cttttttgct gccgccaagc tcttcgtcct ggagtttttc
     5161 cagttccttc tcagcgacaa tttctccgac gcggtctgaa agtttcagcg atatttccgg
     5221 atttgccatc cgtgagcgga ggcggtcgcg ccgggcaaga aaggttgtaa acgccggatt
     5281 tccggccatg cgcatttcgg aatgtttcct cagttcagga atggccggtt taaccgaatc
     5341 cggccacatc cggtaatagg cgctgcgcac ctgcgaccac ggcagcgctt tcggcagcga
     5401 ttcttcaccg atatcaagcg cgtcgtagag tgaaggaagc acaacgtccg gcacgacacc
     5461 tttgagctgc gtggagcctc cggcgatacg gtagaagctg gccgtcgtga ctttgatcga
     5521 ccccagatca tcactgagtt tggaaagggg aaagacggtc tgcaccgtgc ctttaccgtg
     5581 agttttgctg tcgccgacaa tcagagcgcg cccgtagtcc tgcaatgcgg cggcaacaat
     5641 ttcagaggcc gaggcgctga gccggttgac gagtacaacg agcgggccgc tgtataccgt
     5701 gtcggggtcg ccgtcaggca gcacggcaac accgcgctgt tcacgaacct gaacgaccgg
     5761 gccggcggaa atgaagagcc ctgcgatatc aatggcttcc gccagcgaac ctccgccgtc
     5821 attgcgcagg tcgagaatga cgccgtcgac acgtttggcg gcaaactcgc cgagaatgcg
     5881 gcgtacatca acggaagccc gccgtgcgcc gtctttgccg ttccgcgcgg cggcaaagtc
     5941 agcgtaaaat tcgggcaggg tgagtacgcc gatacgatgc ggcacgccgt tggtttcggt
     6001 gatctggcgc acttcgcctt tggccgcctg cgcttccagt tttacttcgt cgcgaatcag
     6061 atcaatggtt ttggtgcgtg ttccggtggt gtcgtcggcg ggaatgattg tcagaacaac
     6121 cttggttcct ttttcgccgc gaatgagacg tacggctttg ctgagcggcc agtagagaat
     6181 acttaccgga gcggcattgc tttgggcgac ggcgatgatt ttatcgccgg cccggagccg
     6241 tccgtccagt tccgccgggc cgccggcaat gagctgtgtg attttcgcca tgccgtcttc
     6301 ggtgcgaagc atggcaccga taccgacgag cgagaggctc atattgatgt caaagtcctc
     6361 gacgccgcgc ggagagagat attcgctgtg cgggtcatag ctgcgggtaa aggcgctcag
     6421 atagcgctgc agaacggttt ccttgtcaaa gttgttgtcg atcagcagct gatattgttt
     6481 ataccgctcg cgaacaaatt caaccggcgt gagcgcctcg attctggctt cgtcgaccgt
     6541 gttggtccct tcgggcttgg gatcgttttt gccggcttct atggcggcga tccgggagac
     6601 ataatcgttc tttacttttt tacgccagag atcattccac tccgtctcat tttccggcca
     6661 cggagcggta tcgcgtttcc accggtactt ttccgcctga tcgagatcca ttggctggtt
     6721 gagcagctga ttaacgtatg ccacgcgatt ggtgaccctc tctttgaacc ggtcaaaaat
     6781 gacctgtgca taggaggtgt cgccggcgac aacctgatta tccagattgg ttgcctgctt
     6841 ctggaattcg gcaatgtcgg acgccagaaa gaatgtatgg tcgtagtcca gtgaatcgat
     6901 atagagcgcc agcgcgttgg tggcaatcga gtcgtcgaat gcattgcggt tcaggtggat
     6961 atgcggcaga cgatcggcaa tccgcgctgc aatcctctcg gacgggatcg ggtcctgcgc
     7021 cagcgcattg gccgcattgg caggaacttc cggtttcccg catccggcca gaacggcggc
     7081 gagcagtaat agatatagcg ggtgtttgcg cataactttc cctttttacc ataaatgccc
     7141 gcagaaacaa gccgcgtccg ccgactttac ctcatttttg gttttatcct ccggcccgca
     7201 gccgctactc tgcggcctga ttttaaaccg gagaaattga atgctcacaa aacgtcctga
     7261 atggaaggcg ctttccgccc attggaaaaa aactcaatca gcacatcttc gagacctctt
     7321 cgccgccgat ccgcagcgag ctgaaaaatt ttcaatgagc gcctgcgacc tgctgctgga
     7381 ctattcaaaa aaccgtatca cagacgaaac aatgagtctg ctggttcagc tggctgaggc
     7441 gtccggactg cgcggaaaaa tcgaggcgat gttcagcggc gaaaagatca accgcaccga
     7501 agaccgcgcc gtgctccaca ccgcgctccg cgctccgcgc ggcacaaagg tttgcgtgga
     7561 cggagaagac atcatgcccg gcattcatgc ggtgcttgat cagatggaga aattttcaaa
     7621 cagcatccgc tccggcgagt ggaaaggcta caccggcagg ccgatccgga atgtgatcaa
     7681 tatcggtatc ggcggttccg atctcgggcc tgtcatggcg tttgaagcgc tcaagccgta
     7741 ttctcagcgc aacctgacga tccgctttgt atccaacgtg gacggcaccc acatcgctga
     7801 agcggtgcgc gacctgaacg ccgaagagac cctgtttatc gttgcttcca aaaccttcac
     7861 gacacaggaa accatgacca acgccgaaac ggcgcgtacc tggctgctgg cggcgctgaa
     7921 agacaaagcg gctgttgcca aacatttcgt ggcgctttcc accagcgccg ccgaagtttc
     7981 gaaattcggt attgatacgg ccaacatgtt cggcttctgg gactgggtcg gcgggcgcta
     8041 ttcactctgc tcggcgatcg gcctgccggt gatgatcgcc atcggtccgc agaatttccg
     8101 taaaatgctg gcgggctttc atgcgatgga ctgccatttc cgcaccgcac cgtttgctga
     8161 aaatatgccg gtgattctcg cgctgctcgg catctggtac aacaacttct tcagcgccga
     8221 gacccaggcg attctgccgt atgatcagta cctcagccgc ttcgccgctt atttccagca
     8281 gggcgatatg gaatcgaacg gcaagtacat tacacaagac ggcaagcggg tgaagtggca
     8341 gaccggcccc gtaatttggg gcgaacccgg aaccaacgga cagcacgctt tctatcagct
     8401 gatccatcag ggcaccaaac tgatcccgtg cgacttcatc ggatttgcca aatcgcagaa
     8461 tccgctgggc gaccaccacg ccaagctgat ggccaatttc ttcgcgcaga cccaggcgct
     8521 ggccttcggt aaaaccgctg aagaactgaa agcagaaaag acgccgaaga aactgattcc
     8581 gcacaagacc ttcgaaggca accgtccgac caataccatc atggcggaaa aactgacgcc
     8641 ggaagtgctc ggccatctga ttgctctcta tgaacagaaa atttttgtac agggaatgat
     8701 ctgggatatt ttctcgttcg accagtgggg cgtgcagctt ggcaaagtgc tggcgtccgc
     8761 cattctgccg gagctggaaa gccgttcgga actcaaacac gatgcttcca ccaaccggct
     8821 cattgaatac ttccggaaaa acaaataacc ggtttggctg gtaaccctgt ggctccgccg
     8881 attccggtgg agcctttttc tttccaagga ttgcaaaaat caatgccttg cgcttccaat
     8941 cgggggcgca aatggctggg aaatattatg ccgggggctt gagtttaaaa gcccgaccgg
     9001 taaactccgc cggatatgaa aattctgctc ttcattggaa tcggcgggtt cctgggctcg
     9061 attgcgcgct acgggatgac tcttggcgta cagcactggg ccggcggcgg acggctgggc
     9121 gactttccgg tcggtacgct ggccgtcaac ctgctcggct gtctggtgct tggttttctc
     9181 ggtcatctgc tgagcgagcg ttttgagatg gcggcggaaa tccgttttgc gcttaccgtc
     9241 gggtttatcg gcgccttcac gaccttcagc acctatgcct atgaaacact ggcaatggcg
     9301 cgcgaaggcg aacagctctt tgcgtggctc aacattctgg ccagcaacgc gctcggtctg
     9361 tttgccgtct ggggcggtta ccggctcggc gggctggtgt gatgattaat ataacgtact
     9421 ccgtattgcg tatttcccgg acgattcaac acgcagtacg aaatacgcgg tacagcttga
     9481 attaaatgcc cgttctggaa taatcgcggc cagttttgag aggacattaa ccatggaaaa
     9541 ctttaccccc agagcccagc gtgtactgca tctggcccgc aaagaggccg agcagttcaa
     9601 ccataactat gtcggcacgg aacatattct gctcggcctt gttgctctcg gcagcggcgt
     9661 agccgtcagc gcgctccagt cgctcggcgt tgatttacag tcgctccgcc tcgaagtgga
     9721 aaaagcggtc ggaaccgggt cggataccaa aatgaccggc aatattccgt ttacgccgcg
     9781 cgccaagaag gtgctggcgc tggcgaccag cgaggcgcgt tcgctgaacc acagctatgt
     9841 cggcaccgaa cacattcttc tcggcctgct tcgcgaaggc gaagggatcg ccgcccgcgt
     9901 acttgaaaat atgggcgtgg atctcgaaga ggcccgcgaa gaaatcatgg cgatgcttga
     9961 tccggactac gacggttcca tggaatctgc gaatgaagaa gaagctccgg cgttcggcgc
    10021 accgcagaaa accgccgaag ggcaggccgt caaaacgccc gcgctgaatg cgttcggccg
    10081 cgatctgact gaaatggcga agaagggcga acttgatccg gtgatcggcc gcaaaaacga
    10141 aatcgagcgc gtcatccaga ttctctgccg ccggacgaaa aacaatccgg ttctgctcgg
    10201 tgaagcgggc gtaggcaaaa ccgcgattgc tgaaggactc gcccaatcca ttgccgacgg
    10261 cacggtgccg cagcttctgg cggataaaaa agtggtcaca ctcgacctgg cgctgatggt
    10321 ggcgggaaca aaataccgcg gacagtttga agagcgcatc aaggccgtta tggacgaaat
    10381 ccgcaaaacg aaaaacgttc tgctgttcct cgacgaaatg cacacgatcg tcggggccgg
    10441 ttccgccgaa ggaaccatgg atgccgccaa tatcatcaaa ccggcgcttt cgcgcggcga
    10501 gcttcagtgc atcggcgcca cgacgctcag cgaataccgc aagtcgattg agaaggatgc
    10561 cgcgctcgaa cgccgtttcc agacggttat tgtcaaagag ccgaccattg aagaaaccat
    10621 ccagattcta aaaggactgc gcccgaaata cgaagaacac caccatacga aaatcagcga
    10681 tgccgcgctg gaatccgctg ctaagctttc ggcgcgttat ctgccggacc ggttccttcc
    10741 ggataaagcc atcgacctga tcgacgaagc gggcgcacga agccatatcg ccaacatggt
    10801 caagccgccg gagtttcagg aaatccgcca gcggatgatg gaagcccagc agaaaaaaga
    10861 taccgcaatc cgcgaacaga agttcgagga agccgcctct atgcgcgatg ccgaacgcaa
    10921 agcgaaggaa gaattcgacg ccgccatgaa agcgtgggaa gcctcccgcc aggagagcct
    10981 ttcgaccgtt gatgaagagg aaatccgcat tgtcgtttcc aagtggaccg gcgttccggt
    11041 ggataaaatg ggagaacagg cgctcgctaa actgcttcag atggaagcgc agatcgaaaa
    11101 gagtgtcatc ggtcagaacg aagcggtttc cgccatttcg cgcgctttgc gccgctcgcg
    11161 cgccgaactg aaagatccgc gccggccgat cggttccttt gcatttctgg ggccgaccgg
    11221 cgtcggaaaa accatgctgg cgaaggagct ggcgcgtttc atgtttgacg atcccgatgc
    11281 gctgatccag atcgatatgt cggagtatat ggagaaattc tccgcttcgc gtctcgtcgg
    11341 ttcgccgccc ggctacgtcg gttacgaaga gggcggacag ctcaccgaac gggtgcgccg
    11401 ccgtccgtat tcggtcgtgc tgtttgatga ggttgaaaag gcccatccgg atgtcatgca
    11461 catgatgctg caaattctgg aagaaggccg cctgaccgac agtcttggac gcagcgtgga
    11521 cttccgcaac accatcgtca ttatgacttc aaatgtcggg gcaacgcatt ccgctaaagc
    11581 cggactgggc ttttcgccga tcagcgagca gaacgattac gacaagctta agggcgtcat
    11641 gatggccgca tgtaaagaaa cctttaagcc ggaactggtc aaccggctgg acgatatcat
    11701 cgtcttccgt cagcttaacg agaaggatat tgaaatcatt ctggatctcg aaatcgcccg
    11761 ggtccgggaa cgtgtcgctg cgcgcaagat cacgctggag ctgtctgccg aggcccggca
    11821 gtttctcatc aagaagggat tcgataaggc ctacggagcg cgtcagcttc gccgggcggt
    11881 tgaacaccag cttgaagatc ctctggccga ggcgatcctg cgcggcgagg tcgtcggcga
    11941 taaaccggtc ggcgtcggcg tcagcggaga gaagctggcc tttacccagg tctgaccggg
    12001 cgcagaaaag cggtttcagg tccggggcgt gtaaaggttc agaatggcct gcatttcttc
    12061 gcggtggatg ggtttcgcca gataatcgct catgcccgct tcgatgaatt tttcgcggtc
    12121 acctttcatg gcatgtgcag tgagcgcgac aatcggaacg gttttgtcat gtttccggat
    12181 tgcggcggtt gcctgcagtc catccatgac cggcatctga atatccataa agatgatgtc
    12241 gtaagcgggg cgctgttccg ggggctctgt cagccggagt ttttcaacgg catcctgtcc
    12301 gtcttcggcg aggtcgatca ggcagccttc tttttcgagc attttcataa tgacgcgctg
    12361 attcagtttg ttgtcttcca ccagaagaat ggatggagcg cgggtaaggg cataggcagg
    12421 gggccgggcg gcttcgctgc tgacggtctg ttctgtctgt tgatccagcg gcacggtgat
    12481 gctgaaggaa aatacggcgc cgtgatcggg ttcgctggta acggtcaggc tgccgcccat
    12541 tttttcaaca atccggcgcg agatcgcaag ccccagtccg gttccgccgt atttgcgggt
    12601 gtgggaacca tccgcctggg tgaattcgcg gaagatgatt ttctgggctt cttttgtaat
    12661 gccgataccg gtgtcctgga tgctgaattc aagcgtacag tctgtaccgg ttttgcgcgg
    12721 acaccgtacc gaaacgcgca catgtccctt gtgtgtgaac ttgatggcgt tggccatgag
    12781 gttgatcaga acctgccgga ggcagctttc atcggcccga atgacccgcg gcacatcctt
    12841 gattccgtcg atgtgcagat caagtccttt ggagtgaacg gcgggagtga agaatttatg
    12901 gagttccatc agcaggtcgg gaacgcaaac gggttcattt ttgagttcga attttccaag
    12961 ttctacacgc gagagatcaa gaatggagtt gatgatcgcc attaattttt cggcagaggt
    13021 gaagatggtc tgaatgtaat ccgcctgttc ggcggtcagc tccgtgtctt ccatgagctg
    13081 agtcatgccg atgatgccgt tcatcggagt tcgtatttcg tgcgacatgt tggccagaaa
    13141 ttcgcttttg ctgcggttgg cggcttctgc attttccttg gcggtttgaa gctcatttct
    13201 taagcgggtg tcttcctggt gacgttcttt gagctgggcg atcatgttgt tgattccgcg
    13261 ttccagcaga ccgagttcat cgggagaatc cgcatggctg atccgggcgt tgtaatttcc
    13321 gccggaaaca gcttttaaac actgtccggc ggttttgatg cgcggtattg tcagcttccg
    13381 gattagaatg actccggcaa aagtaatcag cagaacgcaa aacagtccgc cgctgaaaaa
    13441 cgtggaagcg actgcttttt tctttgcagc ggttcgtccc gtatcgattt ttatatgaag
    13501 ttcacccaaa cgctgattat tcgcgaacag aggcgtggtg atgtggagat ataccgcatt
    13561 gtcctgagcg agaatgctca gcgtttcacc ggccgcgttc tgaactgtac cggcactctc
    13621 caggcacgta ttgtaagtgc cttcacgcgc agggtcagtg cagtaatgaa tgcagtgatc
    13681 gcggcggttc acggatgcat atatgacgtt ttctccaatc aggtcggaca gagccccgcg
    13741 gtctcgaacc gcactgtacg gaagaacctg ctggttcatg agccgccctg gaatccgggc
    13801 ctgagtgtaa agctgtttat caatctgctg agaaaaaagc cgggtgtaat aaattccaag
    13861 tccggtgaag cttaaggcgg cgatcaggaa aattgtcagt ccaagtttga atgccataga
    13921 tctgaaaacc tgtttcattc cgatttctcc tcgaacttgc ggtacgcttc gccggctgaa
    13981 atttcagcgc gcaacagggg tttgagactg cggaacaccg gggtgatcgg gctgtggtct
    14041 gattccgata tgaagatgtc gggtttaagg gtgcggttca atgccagctt gcgctgatac
    14101 tcagcaaaga ggtcattacc gtattccgga tcataaagat ttccggcaag tccggtcggg
    14161 cttttcgtgt agcggatcca tttttcagca atttcggggc ggctccagaa ctgcatcagc
    14221 cggatcgccg ctgcgcggtt cggactgttt ttcattacag cccacacggg tataaatccg
    14281 cccgcataat agcgctgctt ttgaaatccg ggcatttgcg ccagccgcag tttccggagg
    14341 ctctgcggaa atgaatcctt cagcgtgtta taccgccagg tcggttcgat catgaaaaga
    14401 gccttgtttt ccgtcagccg tctggcggtg tccagccagg ttcctttcgg ttcgctgtac
    14461 agcaccggat ggagccggcc cagctttccg aacgtgtcta atacctgccg gatggccggt
    14521 tcggtatttc ccgcagaatc ttccggggcg gcggaattaa acagattata ggccagccgg
    14581 ccaaaggctc cggaattata attatcggca aacaacgaga ccggtacatc ggcagtccgg
    14641 ttgtattcat cgacccggcg ggcatagctc agcagatcat cggcactcat ttcttcttca
    14701 cggatttgaa gtccgagcgc agccgccacc tcggtgttgt accagagtgc atagaaaaaa
    14761 ccttcaatat atggaccggt gaaaacgccg ccggtttgtt tgtgacagtc cggcccgtcg
    14821 acgagaaacg gcttctgtgt ttctttgaaa ccgggaactt ctgcaaaatc gaccagatat
    14881 ttgcgccccc agtccggatc gttcagcagc acggcaactt tccgataaat gagcgcatcc
    14941 atccagacga catcccaggt gatattccct gaccggatca tatctgccag aaaggcggct
    15001 tctgccgact ggcttttttc ctgaagtaca tcggcggaaa atttgaactg aacacgaatg
    15061 ttctgatttt cgaaggtgaa gtcgtccaga acttcgcgta ccagctgttc gcgaagacct
    15121 tcaccctccc agtgccccat ccagcgaagt tgctgaaccg gcccgtcctg tgcaaaaacc
    15181 ggaagaacgt aaaaacacag cagactgatt aaaataaact ttaagtgttt ttgcataatg
    15241 aaataccggc gcgttctacg taatagactg gcgttataca atgaaaaacc tgtgcggaag
    15301 tttctattaa aggaaagcgt tcgcgtcgga tttgaccgaa cagcgtgtat ttctcctttg
    15361 tttccttctc cgccttctgt aaaactcccc gcttccaaac cttggaaata tacgatggaa
    15421 acaattgcaa tactgggaac agggctgatg ggatcgtcac tgggactggc gctgagaaag
    15481 cgcaatgtgc cggtgcggat ccatgtttat gcccgccggc ccgaaacccg tgagactgcg
    15541 ctgaatttcg gcgtagcgga tgctgtcttt tccgatccgg tcgaggctgt caaaggcgcc
    15601 ggtctgattg ttctatgtgt tccggttctg acgattccgg aactggcaaa ggcctgccgc
    15661 gcaggacttc ggccgggtgc gatcatcacc gatgtcggca gtacgaaagc ctgcctgaat
    15721 gaattgatga cggaagtgct ggcaggaacc ggcgccgagt ttatcggctc gcatccgatc
    15781 tgcggttcag agcagcaggg tattgaagcg ggtaacgctg atttgtatga cggagctgtc
    15841 accgtggtga caccgccgat gcttgctgat gatgtgtctg ttgaaaaagt ttccagcctc
    15901 tggaaaagcg ccggtgcgcg cgtgaccatt atggatgcgg cctcacacga ccggattctt
    15961 gcggcgacaa gccatctgcc gcacgtcgtc gcggcggcgc tggcgctttc atccggcgat
    16021 aacggtctgt tctgcggcag cgggttccgc gatacaacac gcattgccga cggctcgccg
    16081 caggtctgga gtgatattgt ccggaccaac gcgccggcat tgaaggcggc gctgaaaatt
    16141 ttctgtgaca atctgaatga actggcggcg ctgattgacg aagataacgg cgaaaagctg
    16201 gcaaactggt tcgccgccgc ccgcgataaa cgcaaggagc tgctccgctg atgaattcaa
    16261 aaacggttta tcctgtttca aaattcggcg gtgaactgcg cgttcccggc gataaaagcg
    16321 tttctcagcg gatcgccatg ctggcggcgc tggccaaagg cacttccgaa gtgcgcggtt
    16381 ttctgcacgg cgaagatgcc atgagcactc tcagtgcgat gtgctcgctg ggcgcctcgg
    16441 caaagttcga aggggatgtg ctgaagatta ccggtaccgg cggaaagttc atggaacccg
    16501 ccaatcctct ggatatgggc aattccggca ccggcacccg tctgctggcc ggcctgctgg
    16561 ccggacagaa aatgactgta accatgacgg gcgatgcatc gctttcgcgc cggccgatgg
    16621 gccgtatcaa aacaccgctg gaactgatgg gtgcgaagct tgatctgacg ggtgagaaag
    16681 gcacgctgcc gctgacaatc cacggtacgc cgctgcacgg catttgctac aaactgccga
    16741 tggcttccgc acaggtgaaa tcctgcgtgc tgctggccgg actttttgcg gaaggcaaaa
    16801 ccacggtgat tgaaccgcgg ccgacccgcg accatactga aaaacttttc cagctgctgg
    16861 aaattccgct gcacattgac ggcctggaaa ttacattgca gggcttcggt ccgaaaggtc
    16921 cgcagatcgc ggcccgcaat ctgacggtgc cgggcgattt ttccagtgcg gctttctgga
    16981 ttgcggcggt agccgcacgg ccgggtgcaa cattgactgt gcgcggcgtg gggctgaatc
    17041 cgcggcgcac agcgctgctg gatgttctga aacggatggg cgcgctgatt gaagttgaag
    17101 tcgcagagga acatggcgac ccgatcggca cggtgtttgt gcgcggtgcg cagctgcgcg
    17161 gaacggaaat cggcggcgat gaaattccca atttgattga tgaactgccg atgctggctg
    17221 taaccggggc gctggccaaa ggtaaaaccg tgatccgcga tgcggcggaa ctgcgggtga
    17281 aagagtctga ccggatcgct gtaacggcgg cgcatctgcg ggccttcgga gttcatgtgg
    17341 atgagcatcc ggacggcatg acggttcacg ggccggcaat gctgcactcg ccggacaatc
    17401 cgctggcatc gcacggcgac caccggatta cgatgtcgat ggcgatgctg gcgacctttt
    17461 ccgatgctcc ggttaaactg gagcaggtcg aatgcgtggc aacatcttat cccgatttct
    17521 ggaaccattt ggagcagctg ggcggaaagg ttgaatgaac agacaacgga ttattgcaat
    17581 tgacgggccg gcggcctcgg gaaaatcgac ggtggcgcgc gaagtggcaa aacggatgaa
    17641 ctgcatttat gtcgattcag gttcactgta tcgcggcgta acctggaaga tgctgcagga
    17701 aggggtctgt acccggagtc cgctgctggt gcttccggtt ctgctgaaat cgaaatggaa
    17761 attcgaagtg cgcaacagcg cggttgtttt cacgatcgac ggtgttgagc cgggggaagc
    17821 gctgcgcggg aaagaagtcc gcgaagcggt ttcggatatc gcggcgatgc cggcggtacg
    17881 caccttcgtt gtaaaccgtc tgcgtaagct gagaaaactg ggatcgcttg cgatggaagg
    17941 ccgcgacatc ggttcggtgg tgtttccgaa gacgccgtac aaattttatc tcgatgccaa
    18001 ccccgaggaa cgggcgaaac gccgccatgc cgaactgctg gcgagtggtg aaacggagaa
    18061 ggctcaggag gttctcgagt cgcttcagcg gcgtgaccag aaggattcat cgcgcaagac
    18121 ggctccgttg aaaattgcga ccggggcgta tgtgattgat acgtcgggat acgatatcga
    18181 aggagtcatc cggatcctga tggatcgcat cgcggagctg gagaagccgc gcagtgacaa
    18241 aatgatcaac ccaatatggt atcgcatcac ctgtaaagtg ttcggtatct gcctgaccgt
    18301 ctgcaatcgg tacaaggtgt tcggcattaa aaatgtgccg aaaaccggcg gagtgattat
    18361 tgcctcgaac catgccagct atcttgatcc gccggctgtc ggcgcgagca gccgcaagat
    18421 gcggatgact cattttatgg cccgggacac gctttttcgc aatccgctga tgggcgcatt
    18481 cctgcggcgg gtcggcgtaa ttccgctcga ccgcgaccgg ggggatgtga aggcgatgaa
    18541 aacggcgata cagcttctga gagacggcgc atccgtggct ctgtttcccg aaggaacccg
    18601 ctcgcccgac ggaaccctgc agcctgcaaa gccaggtgtc ggtttcctgg tggccaaagg
    18661 aaatgctccg gtggtgccgg tttacgttca cggttcgtac gaagcgtggt caaaaaacag
    18721 cggcggattg aagtgtaaac cggtcactgt catttttgga cggctgatca ctcaggagga
    18781 aatccgggga ctcggcgaag ggcgcgacgc ttacggattg atcggcgaac tgattatgaa
    18841 acggattgcg gaattgaagg aaaattttga aaagggactg atcagcgaat aagaagcagg
    18901 cagctgaagt ctcttttgac gggagttagg gtaccgggct tattttcagg agaactttta
    18961 tgagtaaaca ggcgaagacg gcaatttccc cgacgcggga ggaaaattat ccggaatggt
    19021 atcagcaggt ggtgcgcgcc gctgagctgg ccgagaacag cgatgtgcgc ggctgtatgg
    19081 ttatcaagcc gtggggctat gcgctatggg aaaatatccg caccgcgctg gacggttatt
    19141 ttaaagcgac cggtcatgtg aatgcttatt ttccgctgtt tatcccgttg agctatcttg
    19201 aaaaagaggc ggaacatgtc gaagggtttg cgaaggaatg cgcggtggtc acgcatcacc
    19261 gccttgaagc aggtccgaac ggcaagctga ttccggcggg cgaactcgaa gagccgctgg
    19321 tggtccgtcc gacttccgaa acgattatcg gcgcgaccta tgccaagtgg gtgcagagct
    19381 accgcgacct gccgattctg attaatcagt gggccaatgt tgtccgctgg gaaatgcgca
    19441 cgcgtctttt tctgcggacg gctgagtttc tctggcagga agggcacacg gttcacgaaa
    19501 caagtgcgga agcgtgggaa gaaaccgtga agatgctgaa cgtctataaa cagttcgccg
    19561 aagaatatat ggcgatgccg gtgttaatcg gtgaaaaaac agccggcgag cgtttccccg
    19621 gcgcggtgaa tacgctttgc atcgaagcga tgatgcagga ctgcaaggcg ctgcaggccg
    19681 gtaccagcca cttccttggt cagaatttcg ccagagcttc tggcattaag tttcagtcgc
    19741 gtgaaggtcg cgaggagatt gcatggacaa cgtcgtgggg cgtttcaacg cgtctgatcg
    19801 gcggaatgat tatgacccat gcggatgatg acggtatggt catgccgccg cgcctcgcgc
    19861 cgtcgcatgt ggtaatcctg ccgatcattc gtaaagaaga agagcgggaa aaaatcatgc
    19921 agtactgcaa tgaaaccgcc gccatgctcc gcgctcagac gtatgcgggc cgtccggttg
    19981 acgttattgt tgacgggcgc gacatcaatg ccggcgaaaa gggctggagc tgggtcaaga
    20041 agggtattcc gctccgtgtg gaaatcggtc cgcgcgatat ggaaaacggc gctgtatatg
    20101 tggctcgccg cgataagagc gcaaaggaaa aatacggcca gaaccgcgcg gaatttgttg
    20161 ccgcggtcac ggacgttctc gcagatatgc agaaaaatct ctttgatcgt gcgctcgcat
    20221 tccgcgccca aaacacgaag gaaatcgaca gctgggatga atttgtcacg ttctttaaag
    20281 aaggacgcgg aggttttgcg ctggcgcact gggacggcac cgaggaaacg gaacagaaaa
    20341 tcaatgatga attgtctgtt accatccgct gcattccgtt tgactgtgaa caaagcggcg
    20401 caggaaagtg tgttgtcagc ggcaggccga gcaaaggccg cgtagttttc gctaaaagtt
    20461 actaatcctt gtcagggagc gtgcgatggc aaaaggccgg tttaagattc aagggacgaa
    20521 ggattttctg gttgccgcag ttttctgcgg ttttctctgc atctgggcga ttcgtgacgc
    20581 atggtttcct acggaaaaag tgctcaagaa acacccgcat gagatcccgg tcgcttttac
    20641 ggtcagcggc gttgtgaaag acgttcaggt taaggcggga gatgaagtca gcggcaatat
    20701 tgtgctggcc agtctctatg atgaaggcta tcgcgccaaa gtaactgaag ctgaagctgc
    20761 gtttgaagcc gctaaaacgg ccaaagatcc ggcggttgaa aagaaactgg atgtcttgat
    20821 gaaagcacgg gcggatgcag aggcctgttc acttcaaacc gcaaatatca aatggacgac
    20881 gacgcacggt gaagaagttc ttcgcggcat cgtggcgcgg attctggttg aacctgccac
    20941 ccggattgaa gcgggtacgc cgatacttac agttcagcct gccgacacct tttatttgtt
    21001 taataaaacg ctggcgctgc tgagttttat cgggatgatc gttgcgctgg tgtttcacgg
    21061 aatcgcgtcg cgctgaacaa aacatagtgg ataggacttg accgtgcgcg gagcctcact
    21121 aagatggctg gcctgtcttg agttgtaacc tgttacgacc aagtcaattc gattgaaacg
    21181 ggggaagata tgacaaagag agatttagtg gttcgtattg ctgacgagac tggccttatc
    21241 cagcaggatg ttgccgctat tctccagaag tctctggact atatcgtcga agcgctcaa
//
LOCUS       DAOPZO010000018        19896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_2830_length_19896_cov_9.006300, whole genome shotgun sequence.
ACCESSION   DAOPZO010000018 DAOPZO010000000
VERSION     DAOPZO010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 19896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19896
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2830_length_19896_cov_9.006300"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PKI68_02400"
     CDS             <1..783
                     /locus_tag="PKI68_02400"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNX52575.1"
                     /translation="RGYQKALSWIHEKGAKIPLSVETVLKLHKMTRPNLWDSGTFKKD
                     DGEIIEKHPDGRVTLRFKPLSAKETPQAMEQLCMDTVQLLQGREVPPLILLAAFNLDF
                     LCIHPFRDGNGRVSRLLILLLMYHLGFEAGRYISLERIIEQSKERYYDTLRESSQTWH
                     EAEHNPWPYINYLLYTLKELYHEFESRYENVALPLGEKTETVQRAIQSFSGDFHVTEL
                     QKKCPEVSIDMIRKILNRMRKEGEVTCNGRGKLACWRVSGNR"
     gene            908..2083
                     /locus_tag="PKI68_02405"
     CDS             908..2083
                     /locus_tag="PKI68_02405"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNX52576.1"
                     /translation="MSETAKNTYAEMLAGIKSRVQTARVRAGLAANRELVLLYWDIGR
                     LVAERQDAEGWGASIIDRLSSDIRRDFPGLQGFSARNIIRMKSFYLAYGNGLEISPPP
                     VAKLETGIWPQAVAKLDEAADCPSVLQLPWAHNVLLIEKIKDPDKRLWYMRKAIEHGW
                     SRNVLALHIDQQDYERKGLAVTNFSATLPSPQSDLAQELLKDPYIFDFLGIAADAREK
                     EVETALLVHLRDFLLELGGGFAFVGNQVRLDIDDEDYYIDLLFYHLKLRCYFVIDLKT
                     RPFAAEDAGKMNFYLNAVDDLLRHPDDQPSIGLVLCRAKEGSNKMLLEYALRGLQKPV
                     GVSAYQLTRALPDNLKPSLPTVEEIEIEIAEKVEEDKDAETGMIIREWVEKYRTRTG"
     gene            2222..3508
                     /locus_tag="PKI68_02410"
     CDS             2222..3508
                     /locus_tag="PKI68_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52577.1"
                     /translation="MIIKKNKTSDVYGIGRDLPLNYIVRQDVDQRLIDNLTRDKHLVI
                     YGSSKQGKTSLRKHCLKEADYIVVHCSNRWTLGELHAAILKRSGFEITQASTRTASGK
                     NKIVAKFGADIFGVGGAVSGTKEKTESNSVTTAPLELDPEDVNDVIKALDGFKKYIVL
                     EDFHYLPIETQKDFSVALKAFHEQSGICFLIVGVWLEESRLTVYNGDLTGRITGINAD
                     KWSDDELEKVIEAGEQLLNVSFPANFKRDLISNCLESVYIVQESCYQACDKQGIHETQ
                     ESLVEIDKNVDVKTIIRDVVNQQTGRYNSFIAQFAAGFQDTALQMYKWLLYPVLTATA
                     AELERGLSYRKMRESLRKNHPEGNGLNLGNLTQALQATASLQVKKDIKPIVLDYDQTN
                     LQLNVVDRGFLIWIENQSKSDLLERAELPTDKGSIL"
     gene            3562..>4875
                     /gene="argH"
                     /locus_tag="PKI68_02415"
     CDS             3562..>4875
                     /gene="argH"
                     /locus_tag="PKI68_02415"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNX52578.1"
                     /translation="MKTQKTTVGTINEEVLAFTAGRDVELDMNLIAADCVGTAAHVTM
                     LSKMKPAIITAAERQKVIKELNAIVALAAEGKFKIRLADQDVHLAVERTLTEKLGDLG
                     KKVHTCRSRNDQVAVDLRLYAREQMLGTLDEAAALISALLAFAKKNEKVPMVGRTHMQ
                     PGMPSSVGLWAAAHAESLLDDCALLYNAFELNDQCPLGSAASYGVPLPIDRELTADLL
                     GFSRPSHTVLYANNGRGKLESIVLGAMSQVMLSLSRMAQDLMLFTMPEFGYFTLPDEL
                     TTGSSIMPQKKNPDVIELVRARATRVKACELAVFDLIKGSAGGYNRDLQDAKEPFMEG
                     IAMTRACLRVLSPFIQAIRVNKDKLLAGFTPDVFATDRALELVGQGMPFRDAYHYVKE
                     NLGELANLDPVKAIMQKTHLGAPLGIDWELLKDRVSAVTETVREER"
     assembly_gap    4876..4975
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4976..5743
                     /locus_tag="PKI68_02420"
     CDS             <4976..5743
                     /locus_tag="PKI68_02420"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HNX52579.1"
                     /translation="MKTIIYWFSGTGNSLAAAKTLAKQCGDTELIPMAKALRNPPASA
                     ERIGLVFPVYAFCPPALVARFVEKLNAAPGAYIFAVVTYAGTPGGTLAVLRKLLKKRG
                     LDLASSWGVKMPENYPPFGGAPSPEKQQAVNDVAAERIKKIAAELQMSPRGRHDEIKT
                     LWKIVSRLIYPLFRKRAARADRSFRADEKCNGCCICAQVCPVNNVAMLDGHPSWLGHC
                     EQCYACFHWCPQKAVQYGRSAKQHRYHHPAVELSDLF"
     gene            complement(5817..7052)
                     /locus_tag="PKI68_02425"
     CDS             complement(5817..7052)
                     /locus_tag="PKI68_02425"
                     /inference="COORDINATES: protein motif:HMM:NF010308.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HNX52580.1"
                     /translation="MLNKKTPFHLMAKPAGPDCNLACKYCFYLEKEVFFKEQKIHRMN
                     DEVLEAYIKQYCESQNTPEILFAWQGGEPTLMGVEFFRKAVQLQKKYSAGRPVQNSFQ
                     TNGTLIDNEWCKFLAREKFLIGLSLDGPRHIHDKFRIDRGGQPTFDRVMKALKLMKAH
                     RVDFNTLTCVTRQNAQYALEIYDFLKSTGSTFLQFIPIIERKPDDQAAQLGLKLALPP
                     DLAAADDDPRVTPWTVQPQQYGQFLIDIFDEWVRRDVGNVFVQIFDVMLNGWMDMPPP
                     LCVFAERCGSAMIIEHTGDIYACDHFVYPDFLLGNILQTPMTELVHSEKQKKFGSDKA
                     DKLPEWCRKCDVMFICHGECPKHRFITTPTGEPGLNWLCEGYKMFLHHIDPYMRTMAQ
                     LIRSERPAADIMAMVPPKR"
     gene            complement(7107..7793)
                     /locus_tag="PKI68_02430"
     CDS             complement(7107..7793)
                     /locus_tag="PKI68_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNX52581.1"
                     /translation="MIPFFHKPQSPLIESYLAAVKSKLSRQEPVKKMRFVVLDTETSG
                     FDVATDRILSIALFEIIDGQIDIARSRKWIVFQSGMTPTSATAIHGILPSETREGTQE
                     RKVLEELLPLLTGAVVVGHHIRFDAAMLNAAMLRHFKTKFRNRLIDTAAMAMSELIAF
                     HQTGYANQHPPSLDEVCSQLNLPVVARHTAEGDAFLAAQIFLLLGGRIRSRKNRPLQL
                     RDLPVKRFGL"
     gene            complement(7790..9739)
                     /locus_tag="PKI68_02435"
     CDS             complement(7790..9739)
                     /locus_tag="PKI68_02435"
                     /inference="COORDINATES: protein motif:HMM:NF021824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HNX52582.1"
                     /translation="MNTQNLIPDRIANALRQFPPFSMFEEATVLELARVAKVQVVTKG
                     DPVWKQGDPPGDEVLFLARGRVEYYCQTGDRSELIDVRDVGDILGLSALHEGEPYRVT
                     ALAAEDSILYALAWPLVRELLSTNDQARYYVRRHLFWGTRVGRSLPDYGLEATGGGAN
                     ILEAHLKGAHTITLRPPERLLYCRPETPIREAAALMAAKRVPSILITDSERRPLGIVT
                     GSDLVKEVIVRGKDSSLPVSSVMSGPVVTVATNSSTTAAALIMLRKRIGQVCVTEDGT
                     AHTAALDVCTQKDLLVQSGRHPAGFIHEIRLAHSPARFREICDDIGKMAQTYLEAGIS
                     GLLLGEISAELYDELVNQLITISLAEMTDEGIALLDVPWAWIAVGSDGRREQVLRTDM
                     DNALIFASSGNPQQDEKNRRFFLQLASRVISRMVDAGFSRCQGGVMASNPNWCKTDTE
                     WIEELGNVDMLADGAQLLRAMILYDLRHVAGSRTLSDSVRQTVFKAVGGNEMIKKRMA
                     ELIVATPPPLNFFGKFVVEKKGGNEGEFDIKSRGLTPLRDAARLFALHYDLRQHHSTG
                     GRWLELASARPDMAELAGLAREGYDLLLRIRTLTGLQRGDAGRFIDPATLTQLQRGQL
                     VNVFDVQRMVQESVRIRFQPDARNL"
     gene            complement(9752..10483)
                     /locus_tag="PKI68_02440"
     CDS             complement(9752..10483)
                     /locus_tag="PKI68_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52583.1"
                     /translation="MTAFTYAAELKKTFRCYGHFYRLHVKGAEPQLCRSVLEITSLPR
                     ETVGAGTDPNDLFSNSGSARNLPDAVVIMMNPGSSRPIEDGNTDSLLTMPLPAGFQKP
                     LVLTQPDNTQYQIMRIMVSKGWTHVRVLNISDLRDPKSPSFIARTQKLAALPGGEAHS
                     LFCDARTAERETMLRRKPAASFIIGWGQDAGLIPLAKQCLSRISGEKIITVPAGNDPV
                     LTAHPSPMLQKKKEEWLDAILSASS"
     gene            10771..12747
                     /locus_tag="PKI68_02445"
     CDS             10771..12747
                     /locus_tag="PKI68_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNX52584.1"
                     /translation="MAQISLGWLDYSIMAIYVGFVIGIGYALKKYMKSSSDYFLSGRS
                     IPAWITGLAFMSANLGALELVGMAASGAKYGMATCHFYWLGAIPAMVFLAVFMMPFYY
                     GSKARSVPEYLKMRFDERTRCFNSLTFAVMTVFASGISMNALAKLLSSLLNFKMSFAI
                     GPFVLTDYDAYLWICSGVVLLYVLKGGLTSAIYTEVLQFFMIVMGFAPVVYLGLKDVG
                     GWDTMTHALSSVAQNPASLNIKEAGTTYASNAWTSVCQPLISGASGNPMGVDIFAMMF
                     GLCFVLGFGYWCTNFLVIQRAMAAKNMTSARRTPLIAAVPKMFLPLLVILPGMIAASL
                     AFTAKDGYRLPPVLIKSEASAVAAVQVVQTATLDQPAAVTAVKKAIGLKMDDVKVAAL
                     LTAQAARPLPETEIRARLQDAVAENDYDGVILSLVKKYCPMGLLGLALTALLASFMSG
                     MAGNVTAFNTVWTYDLYQAYIAKDKSDAHYMTMGRCITVIGILLSIACAYFARNYSNA
                     MDVIQLVFGFVNAPLFATFLLGMFWKRTTSHGAFFGLVGGTLTSALFHACTLPAGCVP
                     GIKGGYLAAVKYFPSEMAQNFWLASFAFLACFALTVVISLMTKRTKNDTELTGLVYSL
                     TPKLKDGDEKWYLRPAVLGTVLLAVCIVLNIIFF"
     gene            12764..12955
                     /locus_tag="PKI68_02450"
     CDS             12764..12955
                     /locus_tag="PKI68_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52585.1"
                     /translation="MGLDLRWPIGLMFSLVGALLLVNGLLTGPSAKALGINVNLWWGL
                     VLLVFGLLMAFSAWRSRRN"
     gene            complement(13026..13172)
                     /locus_tag="PKI68_02455"
     CDS             complement(13026..13172)
                     /locus_tag="PKI68_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52586.1"
                     /translation="MKAFKAYGTRGIYGRDVRSSSPEIFAALCAGVTPGGAIETVLQP
                     FASP"
     gene            complement(13176..13553)
                     /locus_tag="PKI68_02460"
     CDS             complement(13176..13553)
                     /locus_tag="PKI68_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002886926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNX52587.1"
                     /translation="MKKEIIRTSDAPEPVGPYSQAVKAGGTLYCSGQIPLNPATGAIP
                     DGINAQTRQVLANLSAVLKAGGADFSNVVKTTVFLKDMNDFPAMNAIYAEAFTGTPPA
                     RSTVQAARLPKDVLVEIDAIAVV"
     gene            13661..13945
                     /locus_tag="PKI68_02465"
     CDS             13661..13945
                     /locus_tag="PKI68_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002530052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNX52588.1"
                     /translation="MKTTEQVVTHEETLNRLSRIAGQVQGVRKMVEEKKYCIDIVTQI
                     QAVRSALRSVEMQILKKHMHHCVSDAFASGSPKDADEKMDELLRVMKKQY"
     gene            13959..14201
                     /locus_tag="PKI68_02470"
     CDS             13959..14201
                     /locus_tag="PKI68_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52589.1"
                     /translation="MKKSILVAMLLGAVASVFAAPQTTCPVLGGPVDHKLYADVNGYR
                     IYVCCGGCIAAVKADPAKYIEKMKAAGVELEKTPVK"
     gene            14198..15451
                     /locus_tag="PKI68_02475"
     CDS             14198..15451
                     /locus_tag="PKI68_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX52590.1"
                     /translation="MKRVLISLLLVTAVPLYAERPAEGGASLTLDQAVSLAIRDNARL
                     RSLHAKTRAMQERPAQAGALPNPMFTYSGMDLADRGSWPDSGEKRYMLQQEFPWFGKR
                     ALRAEIAGKDAETMQREFDAAAREVVMTVKETYYDLYATRRVMEITREEEAVLRSILK
                     VAETMYSTGSRSQTDAIKAQTEVTLLKQKMLELQSQENLLQARLNTLLGRRPDNLLEL
                     AAKPPEVAGPEPVHTLFARAAENRPEILGAQAQVERYALEQRLMQKDYLPDYKLGLEY
                     RSLRKEDDMLMFTVSVELPIWQSKYRAGVREAAEMQVYSQAAREAAAQQSSFDVQDAA
                     FKLQTARRTLELYRTELIPQAEARFSASEAGYRTGKTDFMDLLESERFLLNAKTMAAM
                     MESTVGMQAARLERATGAALPAEGK"
     gene            15453..16664
                     /locus_tag="PKI68_02480"
     CDS             15453..16664
                     /locus_tag="PKI68_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX52591.1"
                     /translation="MKKPLFILAALLIGGAAAVLPPAGCGRNSAGHPAGKTIQYHCPM
                     HPAVVSDQPGDCPICGMKLVLMEQEKQPGAAAAPSVPGLTAVSILPEARQRMGLVLGT
                     VERRMLTRNIRTSARIVADETRLFRVTTKVEGWVDKLFVATTGQAVRPGDPLLTIYSP
                     QLVSAQQEYLSAVQSAGWLKDSARQRLRLWDVSDEQIDRLEKTGQVEKTLTLYAPSGG
                     FVTEKTVLAGQKILPGDPLLVVADLSVVWGDADIYESDLPYVQVGMPMELALPYWPGK
                     TFSGKVSFVSPVLDRETRTLKIRLEIPNPDALLKLEMYGDAVLRFSLGEKLTAPESAI
                     MKTGERTYAFRDDGAGKLVPVEVKTGVRSDGYYEVLAGLNEGDRVVTAANFLVDSESS
                     MRAALDALSGK"
     gene            16667..>19896
                     /locus_tag="PKI68_02485"
     CDS             16667..>19896
                     /locus_tag="PKI68_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNX52592.1"
                     /translation="MIRNLIGFSARNRWLVVLLTAAAVAYALFTMKHIRLDAIPDLSD
                     TQVIIYTKWDRSPDIMEDQVTYPIISALLGAPKVKAVRGFSDFGFSYVYVIFEDGTDI
                     YWARSRVVEYLSKIQGNLPSGVTPELGPDATGVGWVFQYALVDKSGQHNLAELRTFQD
                     WNLRYALQSVPGVAEVAGVGGFQKQYQVTVDPNRLQNYGLSLMELTEAIRSSNNEIGG
                     RLVEWNGREYMVRGRGYVKSTDDLGRVVVKTNAQGTPVLLRDVADITLGPQIRRGLAD
                     LNGNGDAVGGIVVMRHGENAMNVIKRVKAKLEELKPSLPPGVEVVTTYDRSELIGKSI
                     DNLKSELLMEMLIVSLVILFFLRHIPSAIVPILTIPASVCLAFIPMYFLGISSNIMSL
                     AGIAISIGVLVDGAIVEVENAYKKLERWQEGGRVGDFHKIRLEALMEVGPSVFFSLLV
                     IAVAFLPVFTLVDQEGRLFKPLAYTKTLAIAIAALLAVTLDPAVRMLFTRMEPFKLKS
                     RRLSGFLTTLFVGKYYPEEQHPVSRVLFALYERPCRWVLRNPKKVIALSALIVATTVP
                     VYLKLGHEFMPPLNEGVILYMPTTLPGISVTEAQRLLETQDRILKSFPEVVSVFGKAG
                     RADTSTDPAPFSMMETTVVLKPAAEWRAKERWYSSKWTPKFVRSVLGHIWPEHVSWEE
                     LVNEMDGALQIPGSINAWTMPIKGRIDMLSTGVRTPIGIKIFGADLAEIERLGKQIEG
                     TLRKVPGTRSIYAERASGGFFVDFVPKREALARYGLTVNDVQNVIMTAVGGENISTTI
                     EGRERYSINLRYPRDLRANMDQLERILVTTAKGVQVPMSEVADISLVNGPAMIRDENG
                     MLAGYVYVDITGRDVGGYVAEAKQVVAKELKLPTGYSLTWSGQYENMLRVQERLKVVL
                     PLTVLLIFLLLYMNTKSVFKASVVMLAVPFSMVGAVWLMYLLGYNISIAAWVGMIALM
                     GLDAETGVFMLLFLDLSYEDAKKNGLLRNMADLHEAIIHGAVKRIRPKLMTVAAASMG
                     LMPIMWSVGTGADLMKRVAAPMVGGLATSFIMELLVYPAIFLLWRSR"
BASE COUNT         4151 a         5386 c         6031 g         4228 t
ORIGIN      
        1 cgcggctatc agaaagcgtt gagctggatt cacgagaaag gggcgaaaat tcctctttct
       61 gttgaaacgg ttctgaagct gcacaaaatg acccgcccga atctatggga tagtggaacg
      121 tttaaaaaag atgacggaga aatcatcgaa aaacatccgg acgggcgcgt aacgctccgg
      181 tttaagccgc tttctgctaa agaaaccccg caagcgatgg aacagctttg catggatacc
      241 gttcagctcc tgcagggccg agaagttcct ccgctgattc tgctggcggc cttcaatctc
      301 gacttccttt gtatccatcc gtttcgcgat ggcaacggac gcgtatcccg tctgctgatt
      361 cttctgctga tgtaccacct tggatttgaa gcgggccgtt acatcagtct ggagcgcatc
      421 atcgaacaga gcaaagagcg ctactatgac accttgaggg agagttctca gacttggcac
      481 gaggcggaac ataatccgtg gccgtacatc aactacctgc tctacaccct caaagaactt
      541 tatcatgaat ttgaatcccg gtatgaaaac gtagcgctgc ctcttggaga aaaaaccgaa
      601 acggtgcagc gggcgattca gtctttctcc ggcgactttc atgtcaccga actgcagaaa
      661 aagtgcccgg aagtcagtat cgacatgatc cgcaagattt tgaaccggat gcgcaaagag
      721 ggggaagtca cttgtaacgg gcgtggaaag ttggcctgct ggcgtgtatc aggtaatagg
      781 taatggtaat aggtaattta ataggtatag tcggccggaa tggaagtcag cctcatcaat
      841 gacggcccgg tcacgctgat tctggaaagc gccgggagat cggaatgacg gattaacgga
      901 tgtttggatg agtgaaacag cgaaaaacac ctacgccgag atgctggccg gaatcaaaag
      961 ccgggtgcag acggctcggg ttcgcgccgg gctggccgcc aaccgggagc tggttctgct
     1021 ttattgggat atcggcaggc tggtcgctga gcggcaggac gccgaaggct ggggcgcgtc
     1081 cattattgat cgactctcca gtgatattcg gcgcgatttt cccggtcttc agggcttttc
     1141 tgcccgcaac atcattcgga tgaaatcgtt ctatcttgct tatggtaatg gtcttgaaat
     1201 ttcgccacca ccggtggcga aattggaaac aggaatttgg ccacaagcgg tggccaaatt
     1261 agacgaagcg gctgactgcc catctgtttt gcaactgccg tgggcacaca acgtcttgct
     1321 gatcgaaaag atcaaagacc ccgacaagcg tctttggtat atgcgcaaag caatcgagca
     1381 cggctggagc cgcaatgtgc tggctcttca cattgaccag caggattatg aacgcaaagg
     1441 gctggccgtc accaatttca gcgcaacgct gccgtcgccg cagtccgatc tggcgcagga
     1501 acttttgaaa gacccgtata ttttcgactt tctcgggatc gccgccgatg cccgcgaaaa
     1561 agaagtggag actgcgctgc tggttcatct gcgcgatttt ctgctggagc tgggtggcgg
     1621 attcgccttc gtcggcaatc aggtacggct ggatattgat gatgaggatt actacattga
     1681 tctgctgttc tatcacctga aactgcgctg ctattttgtc atcgacctta aaacccgtcc
     1741 gtttgccgcc gaagacgccg gaaagatgaa tttttatctc aacgccgtgg atgacctgct
     1801 gcgtcatccg gatgaccagc cgtccatcgg actggttctg tgccgcgcaa aagaaggctc
     1861 gaacaagatg cttctcgaat atgccctgcg cggattgcaa aaaccggtcg gcgtttccgc
     1921 ataccagctc acccgcgccc tgccggacaa cttaaagccc agcctgccga ccgtcgaaga
     1981 aatcgaaatc gaaattgccg aaaaggtcga agaagacaag gatgccgaaa ccggaatgat
     2041 catccgcgag tgggttgaaa aatatcggac gcgaaccgga tgattaaaag ggattatcct
     2101 ttaaggcgag tattaatgta ggtattaaag ggttctcagg gctggaccat agcaccgact
     2161 tttccaagga gtggaggtgc ggtgagaacg atgcaatgcc ctttaattgg aggaaaagac
     2221 catgatcata aaaaaaaata aaacgtctga tgtctatggc attggtcgtg atttgcctct
     2281 taattacatt gtacgtcagg atgtagacca gcgactgatt gataatctga cgagagacaa
     2341 gcatcttgtt atttatggaa gctcaaagca gggaaaaaca tcgcttagaa aacactgtct
     2401 taaggaggca gactacattg ttgtgcattg ctcaaacaga tggactctgg gggagcttca
     2461 tgcagcgatt ctcaagaggt ctgggtttga gattactcag gcgagcacgc gtactgcatc
     2521 ggggaaaaac aagatcgttg caaaatttgg ggctgatata tttggggttg ggggagcggt
     2581 ttcaggaaca aaggaaaaga ccgagagtaa tagcgttaca acagcgccgt tggagttaga
     2641 tcctgaagat gtgaacgatg tcattaaggc tctagacgga tttaaaaaat atattgttct
     2701 agaagatttt cattatcttc cgatagagac acaaaaggat ttctcagttg ctttaaaagc
     2761 ttttcatgag cagtcaggga tttgcttcct tatcgtgggc gtttggctcg aagagagtcg
     2821 gcttaccgtt tataacggcg atttgacagg ccgcataact ggaattaacg cggataagtg
     2881 gtctgatgac gagcttgaga aagtaattga ggctggggaa cagttactga atgtaagctt
     2941 ccctgcaaat tttaagcgcg acttaatttc gaattgtctg gagagcgtct atattgtgca
     3001 agagtcctgt tatcaggcat gtgacaagca agggatacat gagacgcaag agtcgcttgt
     3061 tgaaattgat aaaaacgtcg atgtgaaaac aataattcgg gatgtcgtta atcagcagac
     3121 tgggcgatac aactctttta ttgctcaatt tgctgctggt tttcaagaca ctgcgttgca
     3181 gatgtataaa tggttgcttt atcctgtttt gaccgcgaca gccgcagagc ttgaacgagg
     3241 cttaagttat cggaagatgc gtgagagcct tcgaaaaaat catccagagg ggaatggatt
     3301 gaatctgggt aatcttactc aggcccttca ggcaacggca tcgcttcagg tcaagaaaga
     3361 tataaagccc atcgttctag attatgatca aacaaatctt cagcttaacg ttgtcgacag
     3421 aggattcctt atctggatcg aaaaccaatc aaagtctgat ctacttgaac gagccgaact
     3481 tccgacagac aaaggttcaa ttctataaaa cgaaaatttt gatttccaat gtttggaaaa
     3541 agtgagcgag gaatcggact tatgaaaaca cagaaaacga cggttgggac gattaacgag
     3601 gaagtgctgg cgtttacggc gggacgcgat gtcgaacttg atatgaacct gatcgctgcc
     3661 gactgcgtcg gtaccgccgc gcatgtgacg atgctttcga agatgaagcc ggccattatt
     3721 actgccgccg agcgccagaa agtcatcaaa gagctcaatg caattgtcgc gctggccgcc
     3781 gaaggaaaat ttaaaatccg cctcgccgat caggatgtcc atctggcggt tgagcggacg
     3841 ctgaccgaaa agctgggcga tctcggcaag aaggtgcaca cctgccgcag ccgcaacgat
     3901 caggtcgccg tcgatctgcg cctttacgcc cgcgagcaaa tgctcggcac gctcgacgaa
     3961 gccgccgcgc tgatttccgc gctgctggcc ttcgcgaaga aaaacgaaaa agttccgatg
     4021 gtcggcagaa cccacatgca gccgggtatg ccgtcgtcgg tcggcctctg ggccgccgcg
     4081 cacgccgaaa gtctgctcga cgactgcgct ctgttgtaca acgcctttga actgaacgac
     4141 caatgtccgc tcggctccgc cgccagttac ggcgtcccgc tgccgatcga ccgcgaactg
     4201 acggccgatc tgctcggctt cagccgtccg tcccacacgg tgctttacgc caacaacggg
     4261 cgcggcaagc tcgaatccat tgtcctcggc gcaatgagcc aggtgatgct gtcgctttcg
     4321 cgcatggcgc aggatttgat gctctttacg atgccggagt tcggctactt cacgctgccg
     4381 gacgaactga cgaccggcag cagcattatg ccgcagaaga aaaacccgga tgtgatcgag
     4441 cttgttcgcg cccgcgccac ccgcgtcaaa gcctgcgagc tggcggtgtt cgacctgatc
     4501 aaaggctcgg ccggcggcta taaccgcgac ctgcaggacg ccaaagagcc gttcatggaa
     4561 ggcatcgcga tgacccgcgc ctgcctgcgg gtgctttcgc cgtttatcca ggcgatccgc
     4621 gtgaataaag acaaactgct ggccggtttt acgccggatg tttttgccac cgaccgcgcg
     4681 ctggaactcg tcgggcaggg tatgccgttc cgcgacgcct atcactacgt caaagaaaac
     4741 ctcggcgaac tggcgaacct agatccggtc aaagccatca tgcagaagac acacctcggc
     4801 gcgccgctcg gcatcgactg ggaactgctc aaagaccgcg tatcagccgt gaccgaaacc
     4861 gtccgcgaag agcgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatgaa
     4981 aacgatcatc tactggtttt ccggaacagg gaattcactg gcggcggcca aaacgctggc
     5041 gaagcagtgc ggcgacaccg aattgattcc gatggcgaaa gctctcagga atccgccggc
     5101 atccgccgaa cgcatcgggc tggtgtttcc ggtttatgca ttctgtccgc cggcgctggt
     5161 agcgaggttt gttgaaaaac tgaatgcagc gccgggcgcc tatatttttg cagttgttac
     5221 gtatgcgggc actcccggcg gcacgctggc ggttttgcgg aaacttctga aaaaacgcgg
     5281 tcttgatctg gcctccagct ggggcgtcaa gatgccggag aactatccgc cgttcggagg
     5341 tgcgccctcg ccggaaaaac aacaggctgt taatgacgtt gcggctgaac ggataaagaa
     5401 gatcgccgca gaacttcaga tgtcaccgcg cggccggcat gatgagatta agacgctctg
     5461 gaaaattgtc agccggctga tctatccgtt attccggaaa agagcggcgc gtgccgaccg
     5521 gtcgttccgc gccgatgaga agtgcaacgg gtgctgcatt tgcgcgcagg tttgtccggt
     5581 gaataacgtg gcgatgctgg acggtcatcc gtcgtggctg gggcattgcg agcagtgtta
     5641 cgcctgtttc cactggtgtc cgcagaaggc tgtgcaatac gggcgttcag caaaacagca
     5701 tcgctatcat catcccgccg ttgaactttc agaccttttc tgaggtaggg ctcgatcgcc
     5761 gagcgagccg ctgtccctgt gttcagttga aggacggacg cctcggcgat gcgtccctac
     5821 cttttcggcg gaaccatcgc cataatatcg gcggcgggcc gttcactgcg gatgagctgg
     5881 gccatggtgc gcatgtacgg gtcgatgtgg tgcaggaaca ttttgtaccc ttcgcacagc
     5941 cagttgagtc ccggctcgcc ggtcggggtg gtgatgaagc ggtgtttcgg gcattcgccg
     6001 tggcagataa acatgacatc gcatttgcgg caccactcgg gaagtttgtc ggctttgtcg
     6061 ctgccgaatt ttttctgctt ctcggaatga accagttcgg tcatcggtgt ctggaggatg
     6121 ttgccgagca ggaagtcggg atagacgaag tggtcgcagg cgtatatatc gccggtgtgt
     6181 tcaataatca tggcgctgcc gcagcgctcg gcaaagacac agagcggcgg cggcatgtcc
     6241 atccagccgt tgagcatgac gtcgaagatc tgcacaaaaa cattaccgac gtcgcgccgg
     6301 acccactcgt caaagatatc gatcagaaac tgtccgtact gctgcggctg gacggtccac
     6361 ggcgtaaccc gcgggtcgtc gtccgcggcg gccaggtcgg gcggcagggc gagtttgagg
     6421 ccgagctgcg ccgcctgatc gtcgggtttg cgttcgatga tgggaatgaa ctgtaagaag
     6481 gtgctgccgg tgcttttcag gaagtcgtaa atttccagcg cgtactgtgc gttctgccgg
     6541 gtgacgcagg tcagggtgtt gaagtcgacc cggtgcgcct tcatcagctt gagcgctttc
     6601 atgacgcggt caaaggtcgg ctggccgccg cggtcgatgc ggaatttatc gtggatgtgg
     6661 cgcgggccgt cgagactgag accgatgagg aatttttccc gcgccagaaa tttgcaccac
     6721 tcgttgtcga tgagggttcc attggtttgg aaagagttct gaacggggcg tccggcggag
     6781 tattttttct ggagctggac ggctttccgg aagaattcga cgcccatcag cgtcggttcg
     6841 ccgccctgcc aggcgaagag aatctccggc gtgttctgcg attcgcagta ctgcttgata
     6901 taggcttcca gtacctcgtc gttcatccgg tggattttct gctctttgaa gaaaacctct
     6961 ttttccagat aaaagcagta tttgcaggcg aggttgcagt ccgggcccgc cggtttagcc
     7021 atcagatgaa agggagtctt tttgttgagc atgacccgtg aagtatgccg cttgcgccgg
     7081 tgccgttcaa ggttgcaaag cgcaaattaa aggccgaacc gtttaaccgg caggtcgcgc
     7141 agctgcagcg gacggttttt ccggctgcgg attcgcccgc ccagcagcag gaagatctgg
     7201 gccgccagga aggcgtcgcc ttcagcagta tgccgcgcaa cgaccggcag gttgagctgc
     7261 gagcagactt catcgagcga cggcgggtgc tggttggcat agccggtctg gtggaaggcg
     7321 atcagttcgc tcatcgccat cgcggcggtg tcgatgagcc ggttgcggaa tttcgtttta
     7381 aaatggcgca gcatggcggc gttgagcatg gcggcgtcga agcggatgtg atggccgacg
     7441 acgacggcgc cggtcagcag cggcagcagc tcctccagaa ctttgcgttc ctgggtgcct
     7501 tcgcgggttt cgctcggcag aatgccgtga atggcggtgg cggaggtcgg cgtcatgccg
     7561 gactggaaaa cgatccattt gcggctccgg gcgatgtcga tctgcccgtc aataatctca
     7621 aagagtgcga tggagaggat ccggtcggtg gcgacatcga aaccgctggt ttccgtatcc
     7681 aggaccacaa agcgcatttt tttgacaggt tcctgccggg agagtttgct tttaaccgcg
     7741 gcaaggtagc tctcaatcag cgggctctga ggtttgtgga agaacgggat cataggttgc
     7801 gtgcatccgg ctgaaatctg atgcggacgg attcctgcac catccgctga acgtcgaaga
     7861 cattgaccag ctgtccgcgc tgcagctggg tcaatgtggc cgggtcgatg aaccggccgg
     7921 catcgccgcg ctgcaggccg gtgagggtgc ggatgcgcag cagcaggtcg tagccctcgc
     7981 gggccagtcc ggccagctcc gccatgtccg gacgggcgga ggccagctcc agccagcggc
     8041 cgccggtgga gtggtgctgc cggaggtcgt agtgcagggc gaacagccgg gcggcgtcgc
     8101 gcagcggggt caggccgcgc gacttgatat caaactcgcc ttcgttgccg ccttttttct
     8161 ccaccacgaa ttttccgaaa aaattcagcg gcggcggcgt ggccacaatc agttccgcca
     8221 tacgtttttt gatcatctcg tttccgccga cggctttgaa aacggtctgg cggacggagt
     8281 cgctgagggt ccggcttccg gcaacgtggc gcagatcgta cagaatcatg gctctcagta
     8341 gctgggctcc gtcggccagc atgtccacat ttccaagctc ctcaatccac tcggtatcgg
     8401 ttttgcacca gttgggattc gaggccatca cgccgccctg acagcgggaa aatccggcat
     8461 cgaccatcct gctgatgacg cggctggcca gctgcaggaa aaagcggcgg tttttttcat
     8521 cctgctgcgg attgccggag gaggcgaaaa tcagcgcgtt gtccatgtcg gtgcgaagaa
     8581 cctgctcgcg ccgtccgtcg ctgccgacgg caatccaggc ccacggcaca tcaagaagcg
     8641 cgattccttc atccgtcatt tcggccagcg aaatcgtaat cagctggttg acgagttcgt
     8701 cgtagagctc ggcgctgatt tcgccgagca gcaggccgga gatgccggct tcgagatagg
     8761 tctgggccat tttcccgatg tcgtcgcaga tctcgcggaa ccgtgccggt gaatgggcga
     8821 gccggatttc atggatgaat cctgccgggt ggcgtccgct ttgcaccagc aggtctttct
     8881 gggtacagac gtcaagtgcc gccgtatggg cggtgccgtc ttccgtcacg cagacctgtc
     8941 cgatacgctt gcgcagcatg atcagcgcgg cggcggtcgt gctggagttt gtggccacgg
     9001 tgacgaccgg cccggacatg acgctcgaaa ccggaagaga gctgtctttt cctctgacga
     9061 ttacctcttt aaccagatcg ctgccggtga caatacccag cgggcggcgc tcgctgtcgg
     9121 taatcagaat cgaaggcacc cgtttggcgg ccatcagggc ggcggcttcg cggatcggcg
     9181 tttcagggcg gcagtacagc aggcgttccg gcgggcgcag agtgatcgtg tgtgcgcctt
     9241 tcaggtgagc ttccagaatg ttggcgccgc cgccggtcgc ttccagtccg tagtcgggaa
     9301 gtgagcgtcc tacgcgggtt ccccagaaaa ggtggcgacg gacataatag cgggcctgat
     9361 cgttggtgct gagcagttcg cggaccagcg gccatgccag cgcgtagagg atggagtctt
     9421 ccgccgccag cgctgtcacg cggtacggct cgccttcgtg cagcgccgac agcccgagaa
     9481 tatcgccgac gtcgcgcacg tcgatcagtt cgctgcggtc gccggtctgg cagtaatatt
     9541 cgacccgtcc gcgggcgagg aacagaactt cgtcgcccgg cggatcgccc tgtttccaga
     9601 ctgggtcgcc ttttgtcacg acctgtactt ttgcaacgcg ggccagttca agaacggtcg
     9661 cctcctcgaa cattgaaaaa ggcggaaact gcctcaaggc gttggcaatg cggtctggaa
     9721 tgagattttg agtgttcata aatcgggcgt attatgaaga ggcggacaga attgcgtcca
     9781 gccattcttc tttcttcttc tgcagcatcg gactgggatg tgcagtcaga accggatcgt
     9841 taccggcggg tacggtgatg atcttttcgc cggagatccg gctcaggcac tgcttcgcca
     9901 gcggaatcaa accggcatcc tgaccccagc cgataataaa ggacgcggcg ggtttgcggc
     9961 gcagcatggt ttcacgctcg gccgtccggg cgtcacagaa caggctgtgc gcttcgccgc
    10021 cgggcagggc ggccagcttc tgcgtccggg cgataaagct cggacttttc gggtcgcgca
    10081 ggtcggagat gttcagtacg cgaacgtgcg tccacccttt ggaaaccatg atgcgcataa
    10141 tctgatactg cgtattgtcc ggttgcgtca gcaccagcgg tttttgaaat ccggccggca
    10201 gcggcatcgt cagcagactg tccgtgttgc catcttcaat cggacgcgac gaaccggggt
    10261 tcatcataat gacgacggcg tccggcagat tccgcgccga gccggaattg ctgaaaaggt
    10321 cgttcggatc agttccggca ccgacggttt cgcgaggcag agaagtaatt tccagcacgc
    10381 tgcggcagag ttgaggttca gcacctttga cgtgcaggcg gtagaaatgt ccgtaacacc
    10441 ggaaggtttt cttcagttcg gcggcgtagg taaatgcggt catagggcgg atttaaccat
    10501 taaaatcccg aacggtgcga aaacaaattt tccattcgtc cggaaatccg ttctgtctgc
    10561 aggctctgaa aaggctgttt gttactggcc ggcataaaca gataaaaaat tgtaaccagc
    10621 gaaaactgta cagctcgccg gtatatctgg tagcgtcacc ggctgtataa tcgattccgg
    10681 cagcgcagtg gatacggaac cggtttgaat gccgtttatt tttactgaat caaatgtagg
    10741 aaattaaaaa tcgcgggaaa ggaactggat atggcgcaga tcagtttggg gtggttggac
    10801 tattcgataa tggccattta tgtggggttt gtgatcggga tcggttacgc cctgaagaag
    10861 tacatgaagt ccagttcgga ctattttctc tccggccgct cgatcccggc ctggatcacc
    10921 ggtctggcgt tcatgtccgc caatctcggg gcgctggaac tggtcggtat ggccgccagc
    10981 ggcgcaaagt acggtatggc gacctgccat ttctactggc tcggtgcgat tcccgcgatg
    11041 gtgtttctcg ccgttttcat gatgccgttt tactacggct ccaaagcgcg gtcggtgccg
    11101 gaatatctca agatgcgctt tgacgaacgg acgcgctgct tcaactccct gactttcgcg
    11161 gtgatgaccg ttttcgcctc cggtatttcg atgaatgctc tggcgaagct gctcagctcg
    11221 ctgctcaact tcaagatgtc ctttgccatt ggcccgtttg ttctgacgga ctacgacgcc
    11281 tatctctgga tctgctccgg cgtggtattg ctctatgtgc tcaaaggcgg actcacctcc
    11341 gcgatctata cagaagttct ccagttcttc atgatcgtga tgggctttgc tccggtggtc
    11401 tatctcggcc ttaaagatgt cggcggctgg gatacgatga cgcatgcgct gagcagcgta
    11461 gcacagaatc cggccagtct gaatatcaag gaggcgggca cgacctatgc atccaacgcc
    11521 tggaccagcg tctgtcagcc gctgatcagc ggcgcatccg gcaatccgat gggtgtggat
    11581 attttcgcca tgatgttcgg tctctgcttt gtactcgggt tcgggtactg gtgtaccaac
    11641 ttcctcgtca ttcagcgcgc catggccgcc aagaacatga cgtccgcccg ccggacgccg
    11701 ctgatcgccg ccgtgccgaa aatgtttctg ccgctgctgg tgatcctgcc cggtatgatc
    11761 gccgcttcgc tggccttcac cgccaaagac ggctatcgcc tgccgccggt gctgattaag
    11821 agtgaggctt cggcggttgc cgccgtgcag gtcgtacaga cggccacgct ggatcagccg
    11881 gctgctgtga cggcggtcaa aaaagccatc ggcctcaaaa tggacgatgt caaagtggcc
    11941 gcattgctga cagctcaggc agcccggccg ctgccggaaa ctgaaatccg cgcccgtctg
    12001 caggatgcgg ttgcagagaa tgactacgac ggcgtgattc tttcgctggt gaaaaaatac
    12061 tgcccgatgg gtctgctggg actggcgctg accgcgctgc tggcctcctt catgtccggt
    12121 atggccggta acgtcaccgc gttcaacacc gtctggacct acgacctcta tcaggcctac
    12181 attgccaaag ataagagcga tgcgcattac atgaccatgg gccgctgcat caccgtgatc
    12241 ggcattctgc tcagtatcgc ctgcgcctat tttgcacgga actattccaa cgcgatggac
    12301 gtgattcagc tggtgttcgg atttgtcaac gcgccgctct tcgccacgtt cctgctgggc
    12361 atgttctgga aacgcaccac cagccacggc gccttcttcg gtctggtcgg cggtacgctc
    12421 acctcggcgc tgttccatgc ctgcactctg ccggccggct gcgtaccggg catcaagggc
    12481 ggctatctgg ccgctgtgaa atatttcccc agcgaaatgg cccagaactt ctggctggcc
    12541 agctttgcct tcctggcctg ttttgcgctt accgtcgtca tttcactgat gacgaaacgc
    12601 acaaagaacg ataccgaact gaccgggctg gtctattcgc tgacgccgaa gctcaaagac
    12661 ggagacgaaa agtggtatct gcgtccggcg gtgctgggaa ccgttctgct ggctgtttgc
    12721 atcgtactga acattatttt cttctaagga aaggaacaca gtcatgggtc ttgatcttcg
    12781 ctggccgata ggccttatgt tctcactggt gggcgcgctg ttgcttgtga acggactgct
    12841 taccggtccc agtgccaaag cattgggcat taatgtaaac ctgtggtggg gtctggttct
    12901 gctggtgttc gggctgctga tggccttttc cgcctggcgc agtcgccgca attgacctgc
    12961 gctgcatcag ttttagccgt cgcggcggga aaccgccgcc ggctttgccg gcagcgcccc
    13021 gctccttacg gtgaagcaaa aggttgcagg accgtttcaa tggcgccgcc cggagtgacg
    13081 ccggcacaga gcgcggcgaa aatttccggc gaactggagc ggacgtcgcg cccgtagata
    13141 ccgcgggtgc cgtacgcctt aaatgctttc atggattaaa caacggcgat ggcgtcgatt
    13201 tcaaccagca cgtctttggg aagccgggcg gcctgcaccg ttgagcgcgc gggcggcgtt
    13261 ccggtaaaag cttcggcata gatcgcattc atggccggga agtcgttcat gtctttcagg
    13321 aagacggtgg ttttcacgac gttcgaaaaa tccgcgccgc cagctttcag tacagcgctt
    13381 aaatttgcca gaacctgccg cgtctgggca ttgattccgt ccggaattgc gccggtggcg
    13441 ggattcagcg gaatctggcc ggaacagtag agtgtgccgc cggctttgac ggcctgggaa
    13501 taagggccga ccggttcagg ggcatctgac gtgcggatga tttctttttt cataaggtct
    13561 ttttacctgc ccgggatgaa tccgtcgagc cgtttgacta aacccgacca ttccggtttc
    13621 ttttgttgac gcataccggt atggggtata ttaaagcggt atgaaaacga cggaacaggt
    13681 agtcacgcac gaggaaactc tcaaccggct gtcgcggatt gccggccagg tgcagggcgt
    13741 ccggaagatg gtcgaagaaa aaaaatactg catcgacatc gtcactcaga ttcaggccgt
    13801 ccgctccgcg ctgcgctcgg tggaaatgca gattctcaaa aaacacatgc atcactgcgt
    13861 cagcgacgcc tttgcatccg gttcgccgaa agacgccgat gaaaaaatgg atgaactgct
    13921 gcgcgtgatg aagaaacaat actgaaaagg agggggccat gaagaaaagc atactggttg
    13981 caatgttatt gggtgccgtg gcgtctgtgt tcgccgcacc tcagacgacc tgcccggtgc
    14041 tgggcgggcc ggttgatcac aagttgtacg cagatgtaaa cggttaccgc atttacgttt
    14101 gctgcggcgg atgtatcgct gcggtgaagg ccgatccggc taaatacatc gagaagatga
    14161 aagccgccgg cgtggaactt gaaaaaacgc cggtgaaatg aagcgcgttc tgatcagcct
    14221 gctgctcgtg acggccgttc cgctgtacgc ggagcggccg gcggagggcg gcgcgtcgtt
    14281 aacgctcgat caggccgtat cgctggcgat tcgcgacaac gcccggctgc gcagtctgca
    14341 cgcgaaaacg cgggcgatgc aggagcggcc cgcgcaggcc ggcgcgctgc ccaatccgat
    14401 gttcacctac agcggcatgg atctggcgga ccgcggcagc tggccggaca gcggcgaaaa
    14461 gcgctatatg ctgcagcagg agtttccgtg gttcggcaag cgggcgctgc gcgcagagat
    14521 cgccgggaag gacgccgaga cgatgcagcg cgaattcgat gcggcggcgc gcgaggtggt
    14581 gatgacggtg aaggaaacct attacgatct ctatgccacc cggcgggtga tggagattac
    14641 acgcgaggaa gaagccgttt tgcggagcat tctgaaggtt gccgagacga tgtactccac
    14701 cggcagccgt tcgcaaaccg acgcgatcaa agcgcaaacg gaagtgacgc tgctcaagca
    14761 gaagatgctg gagctgcagt cgcaggagaa cctgctccag gcccggctga atacgctgct
    14821 tggccggcgg cccgacaatc tgctggagct ggccgcaaag ccgccggaag tcgccgggcc
    14881 ggaaccggtg catacacttt ttgcgcgggc ggcggaaaac cgtcctgaaa ttctcggcgc
    14941 ccaggcgcag gtcgagcgct atgcgctgga gcagcggctc atgcagaaag actacctgcc
    15001 ggactataag ctcggcctgg aataccgcag cctgcgcaag gaggacgata tgctgatgtt
    15061 caccgtcagc gtcgaactgc cgatctggca gtcgaaatac cgcgccggcg tccgggaagc
    15121 ggccgaaatg caggtgtaca gtcaggcggc ccgcgaagcc gccgcacagc agagctcgtt
    15181 cgatgtgcag gatgccgcct tcaaactgca aacggcgcgg cggacgctgg agctttaccg
    15241 cacggaactg attccgcagg ccgaagcgcg cttcagcgcc agcgaggccg gataccgcac
    15301 cggcaaaacc gatttcatgg atctgctcga aagcgaacgc tttctgctca acgcaaaaac
    15361 catggccgcg atgatggaaa gcaccgtggg catgcaggcc gcccggctgg aacgcgctac
    15421 cggagccgcg ctgcctgcag aaggaaaata acatgaagaa gcctctgttc atcctggcgg
    15481 ctctgctgat cggcggggcc gcggcggttc tgccgccggc gggctgcggc cgaaacagcg
    15541 ccgggcatcc ggccggtaaa acgatccaat accactgccc gatgcacccg gcggtggttt
    15601 ccgatcaacc cggcgactgt ccgatctgcg gcatgaagct tgtgctgatg gaacaggaaa
    15661 aacagccggg cgcagccgcc gcgccgtccg ttccggggct gacggcggtg tcgattctgc
    15721 cggaggcccg gcagcgaatg ggtcttgtgc tgggaacggt tgagaggcgc atgctgacgc
    15781 gcaatatccg gacttccgcg cggatcgtcg cggacgaaac gcggctcttc cgggtgacga
    15841 ccaaggtcga aggctgggtg gataaacttt ttgttgcgac caccggccag gcggtgcgac
    15901 cgggcgatcc gctgctgacc atttacagtc cgcagctggt gtccgctcag caggaatacc
    15961 tcagcgccgt gcaatccgcc ggctggctga aggactccgc ccgccagcgg ctccggctct
    16021 gggacgtgag cgatgaacag attgaccggc tggaaaaaac ggggcaggtg gaaaagacgc
    16081 tgacgctcta tgcgccgtcg ggcgggtttg tgacggaaaa aactgtgctg gcggggcaga
    16141 agattctgcc gggcgatccg ctcctggtcg tggccgatct ttcagtggtc tggggcgatg
    16201 ccgatattta tgaatccgac ctgccgtatg ttcaggtcgg catgccgatg gagctcgcgc
    16261 tgccgtactg gcccggaaag accttcagcg gcaaggtgag ttttgtatcg ccggtgctcg
    16321 accgggaaac gcggacgctc aagatccggc tggaaattcc gaatcctgac gcgctgttga
    16381 agttggagat gtacggcgac gccgtactcc gcttttctct gggcgaaaag ctgaccgcgc
    16441 cggaatcggc catcatgaaa accggcgagc ggacctatgc tttccgcgac gacggggccg
    16501 gaaagctggt tccggtcgaa gtgaaaaccg gcgtgcgcag cgacggttat tatgaagttc
    16561 ttgccggact gaacgagggc gaccgcgttg tcaccgccgc gaattttctg gtggattcgg
    16621 aatcgtcgat gcgcgcggcg ctcgacgcgc tgtcgggaaa ataatcatga tcagaaacct
    16681 catcgggttc agcgcgagga accgctggct ggtcgttctg ctgacggcgg cggccgtcgc
    16741 gtatgccctc ttcacgatga agcacatccg gctggatgcg attccggacc tgagcgacac
    16801 gcaggtgatt atttacacca agtgggaccg cagcccggac atcatggaag atcaggtgac
    16861 ctatccgatc atctccgcgc tgctcggcgc gccgaaggtc aaagccgtgc gcgggttcag
    16921 cgatttcgga ttttcctatg tgtacgtcat ctttgaggac ggcaccgata tttactgggc
    16981 gcgcagccgc gtggttgaat atctgtcgaa gatccagggc aacctgccgt ccggcgtgac
    17041 gccggagctc ggcccggacg ccaccggcgt cggctgggtg tttcaatatg cgctggttga
    17101 taagtccggc cagcacaacc tcgcggaact gcggacgttt caggactgga atctgcgcta
    17161 cgcgttgcaa agcgttcccg gcgtcgccga ggtggcgggc gtcggcggat tccaaaagca
    17221 gtatcaggtc accgtcgacc ccaaccggct ccagaattac gggctgtcgc tgatggagct
    17281 gacggaggcg atccgcagca gcaataatga aatcggcggg cggctggtcg agtggaacgg
    17341 acgggaatac atggtgcgcg gccgcggcta tgtaaagtcc acggatgacc tgggccgggt
    17401 cgtcgtcaaa acgaacgcgc agggtacgcc ggtgctgctg cgcgatgtgg ccgatatcac
    17461 gctcggcccg cagatccgcc gcgggctggc cgacctcaat gggaacggcg acgcggtcgg
    17521 cggaatcgtt gttatgcgtc acggcgaaaa cgccatgaac gtgatcaagc gggtgaaagc
    17581 caagctggaa gagctcaagc cgtcgctgcc gccgggcgtc gaagtggtca ccacctacga
    17641 ccgctccgag ctgatcggta aatccatcga caacctgaag tcggaactgc tgatggaaat
    17701 gctgatcgtc agtctggtga tcctgttttt cctgcggcac attccgtcgg ccatcgtgcc
    17761 gatcctcacg attccggcat cggtctgcct cgcgttcatc ccgatgtatt ttctgggcat
    17821 cagctcgaac atcatgagtc tggcggggat cgcgatttcc atcggcgttc tggtggacgg
    17881 cgcgattgtg gaggtcgaaa acgcctataa gaaactggag cgctggcagg aaggcggccg
    17941 cgtcggggac tttcataaaa tccggctcga agcgctgatg gaagtcggcc cgtcggtttt
    18001 cttttcgctg ctggtgatcg ccgtggcgtt cctgccggtc tttacgctgg tcgatcagga
    18061 aggtcggctg ttcaaaccgc tggcctacac caagacgctg gccatcgcga ttgcggcgct
    18121 gctggccgtc acgctcgacc cggcggtccg gatgctcttc acccgcatgg agccgttcaa
    18181 actgaagtcg cggcggcttt ccggttttct gacgactctg tttgtcggga aatattatcc
    18241 ggaagaacag catccggtga gccgcgtgct gttcgcgctc tacgaacggc cctgccgctg
    18301 ggtgctgcgc aatccgaaaa aagtcatcgc gctgtcggcg ctgatcgttg cgaccaccgt
    18361 gccggtttat ctgaaactcg gacacgaatt tatgccgccg ctgaacgaag gcgtcatcct
    18421 ttacatgccg acgaccctgc cgggcatttc ggtgacggaa gcccagcgcc tgctggaaac
    18481 gcaggaccgg attctgaaat cgttcccgga agtcgtctcg gtgtttggaa aagcgggccg
    18541 agccgatacc tccactgatc cggcaccgtt ctctatgatg gaaaccaccg tcgtgctgaa
    18601 accggccgcc gagtggcgcg ccaaggagcg ctggtattct tcgaagtgga cgccgaagtt
    18661 cgtgcggtcg gtgctcggcc atatctggcc ggaacatgtt tcgtgggaag aactggtcaa
    18721 tgaaatggac ggcgctttgc agattcccgg cagtatcaac gcctggacga tgccgatcaa
    18781 aggccgcatc gatatgctct ccaccggcgt gcggacgccg atcggcatca aaatcttcgg
    18841 cgcggatctg gccgagatcg aacggctcgg caagcagatt gaaggcacgc tgcggaaggt
    18901 tcccggcacc cgcagtattt atgcggagcg cgcttccggc ggtttcttcg tcgatttcgt
    18961 gccgaagcgc gaagcgctgg cccgctacgg cttgaccgtc aacgacgttc aaaacgtcat
    19021 catgaccgcc gtcggcggcg aaaatatttc gaccaccatc gaagggcgcg aacggtactc
    19081 gatcaacctg cgctatccgc gcgacctgcg ggccaacatg gatcagctcg aacggattct
    19141 ggtgacgacc gcgaagggcg tgcaggttcc gatgtctgaa gtcgccgaca tttcgctggt
    19201 caacggcccg gcgatgatcc gcgacgaaaa cggcatgctg gccggatacg tttatgtgga
    19261 catcaccggc cgcgacgtcg gcggctatgt cgccgaagcc aagcaggtcg tggcgaaaga
    19321 actgaaactg ccgaccggct attcgctgac gtggagcggc cagtacgaaa acatgctccg
    19381 cgttcaggag cggctgaaag tcgtgctgcc gctcaccgtt ctgctgatct tcctgctgct
    19441 ctacatgaac acgaagtccg ttttcaaagc gtcggtcgtc atgctggccg tgccgttctc
    19501 tatggtcggc gcggtctggc tgatgtatct gctcggctac aacatctcca tcgccgcatg
    19561 ggtcggcatg attgcgctga tggggctcga cgccgaaacc ggcgtcttca tgctgctgtt
    19621 cctcgacctg tcgtacgagg acgcaaagaa gaacggcctt ctgcgcaaca tggccgacct
    19681 gcacgaagcg atcattcacg gcgcggttaa acgcatccgc ccgaaactga tgacggttgc
    19741 cgccgcctcc atgggtttga tgccgattat gtggtccgtc ggaaccggcg ccgatctgat
    19801 gaagcgcgtc gccgccccga tggtcggcgg actcgccacc tcatttatca tggaactgct
    19861 ggtctacccg gcgatcttcc tgctctggcg ctcgcg
//
LOCUS       DAOPZO010000019        19326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_2959_length_19326_cov_9.654922, whole genome shotgun sequence.
ACCESSION   DAOPZO010000019 DAOPZO010000000
VERSION     DAOPZO010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 19326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19326
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2959_length_19326_cov_9.654922"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PKI68_02490"
     CDS             <1..771
                     /locus_tag="PKI68_02490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX52593.1"
                     /translation="LRRQTFQDIELFAVDSGSTDGSLDALRAAVDPAHLVCIPPKAYV
                     PGVVLNDMTARTTQEFIVFQNADAIFQSDDALEKLLQPLFSGEADAVMSAQVTRPDAR
                     FIVTYDYLRAYNPKNIKGDNVDFFSAVTCAFRRDLWEKIKIPNIGYAEDVAWAKLCRT
                     QGARFKLVTDSVVEHSHNYTLKGLYRKKFRHGVTFAHTYGRRTNLLLQTLDLCKEWIR
                     DLFYAVRKGRLDTIPYNIAYRAVIHTGLYRGLKEGARG"
     gene            764..1873
                     /locus_tag="PKI68_02495"
     CDS             764..1873
                     /locus_tag="PKI68_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52594.1"
                     /translation="MGKYSVKGSPAFDARIDADMKRIADAVYASHFSRHWKALVLLGG
                     YGRGEGSPMIGPNGEELPFNDYDLIVVTDSLDPLLKRALKKLEKELSEQVGLPVDLYP
                     YLSSALPKCEFSLLNYELRYGHRVIRGDKKILDAMPAYPHDAIPLSEGTRLLMNRGKL
                     LLDIKRRLARPDTLTHEERQRFLKFLFKADLAFGDCALLLRGAYDLSYVVKRHRLPET
                     DLSGLENSRDFVSAYSRAIDFKERADFKPLDNSNIHLQFDETVRRYEYFFLWYEQRRL
                     NRKFHTLGKYARTFPLLGNEGSPVKNAVHNLRAFGPGALPYCFAHPRLRLYAALPLLL
                     TKQADREEIRWLLNSRESGTEELYKSFYALQQRFS"
     gene            1870..3024
                     /locus_tag="PKI68_02500"
     CDS             1870..3024
                     /locus_tag="PKI68_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNX52595.1"
                     /translation="MKKQKLSLFIFIDAFGREIFLRHPFFLQGLIRDHKKLDTILGYS
                     SACDPSIISGKLPCEHLMWSSFYYDPKGSPFKWTKALAILPDAVFRRGRVRHYLSRLI
                     KKVLGFTGYFQLYGVPFKYLPLFNYAEKKRIWEPGGLLRGETIFDELTRRGIPHYVHD
                     SAVSDETKLARLTADIKSQRIEFAYCSLGKLDALMHSVGTKDQKVTELVHWYDQQVRK
                     LIAAADEQYDEVAWYVFTDHGMHDITEGYDLISDIEKTGLRWNRDYVAFYDSTMARFW
                     FLNETARQKITALLGSHPKGRILDDEELKREGVFFPDGMYGELIFLINSGIQIVPGFM
                     GVKQIKGMHGYHPQDADSAASICSNRELPAGLTKIHQIYHLMHKELGFSE"
     gene            3017..3679
                     /locus_tag="PKI68_02505"
     CDS             3017..3679
                     /locus_tag="PKI68_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX52596.1"
                     /translation="MSDKPQELSAKEKDAVLIVDDDAFMLKVLYRVLQKEYALYQAGS
                     AEEAIEVLRGRHVKAILCDHVLPGQSGLDFLITLKQRNSQIKSVLFSAALETEQFIKA
                     INEGQVFRFVKKPASPADIQQAVREAVRQYDIESLQQQLLINQKDIDKQVYSAPYWLY
                     RLRVIAQQSFRYGAPLFGNILALMLALGVIALSVGICIMLIIYFVKSYLGIDIFQGHL
                     LH"
     gene            complement(3774..5819)
                     /locus_tag="PKI68_02510"
     CDS             complement(3774..5819)
                     /locus_tag="PKI68_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNX52597.1"
                     /translation="MANPTDLFGENVFSMKVMREHLSEATVKSLVATIKTGKSLDAGI
                     ADEVAEAMKEWAVSKGATHYTHWFQPLTGSTAEKHDSFIVPDGEGSVVCKFSGAELIQ
                     GEPDASSFPSGGLRATFEARGYTGWDPSSPAFIKDNTLCIPTVFCGYHGEALDKKTPL
                     LRSMKVLSTQAERLAKLFGIQIKQKAYATLGAEQEYFLIDEELYNARLDLRQTGRTLF
                     GSKPAKHQQMEDHYFGAIKTRVLAFMEDLDAELWRMGVPSKTRHNEVAPAQFEMAPVF
                     EELNLAVDHNMIAMEVMRKTAEKHGFVCLLHEKPFAGVNGSGKHNNWSLCGPDGKNWL
                     SPGDNPHENAKFMTMICALMKAVDTHADLLRATVATAGNDHRLGANEAPPAIISIFLG
                     DQLTDIVEQIEKGGAKSSKTGGYIHLGVDTLPALPRGNTDRNRTSPFAFTGNKFEFRA
                     VGSNQSCAGSNVALNTIVAEALDEICTKLEADVKAGKDFNESLQKLLAGLIKKHKRIL
                     FNGDNYTEAWAKEAARRGLPNVKKTPDALKAFNTEKAKKLFSKYGVLSEKELHSRFDI
                     YNETYEKTIEIEAGVALTMAKTMIIPAAVTAQAELAASIKAVTGCAVTGAKAELKAMC
                     SETDKLYKAVAALEKAHGAEKLIAAMGKVRASVDALEKMVPDDIWPVPTYAEMLFMM"
     gene            complement(6045..7073)
                     /gene="lpxD"
                     /locus_tag="PKI68_02515"
     CDS             complement(6045..7073)
                     /gene="lpxD"
                     /locus_tag="PKI68_02515"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:TIGR01853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNX52598.1"
                     /translation="MKLSEVAEKTGGIVDGDGNIDIRGVAGIGEAVEGEISFIANQRY
                     ASAAAATKASAVIVSENWSSACSAALIRAKNPDETFAKAAMLFYTPPPRPAIGVHPSA
                     SVAADAEIGENVSIGAFCLVESGAKIGAGTVVFPQCYIGRKAVLGENCWLYPQVSLRE
                     AVILGKRVIVHNGTVIGSDGFGYSVDGQGIRTKISQIGIVEIGDDVEIGANVTIDRAR
                     FGKTRIGHGVKIDNLVQIAHNVVIGDHSVLVAQVAIAGSTHIDEKVIIAGQAGVAGHV
                     HIGAGAVIGAKAAVTKDVAAGEYMIGVPAMPAAKFKRTQAGIMLLPKLKERVAELALR
                     IRNLEQSE"
     gene            complement(7070..>7312)
                     /locus_tag="PKI68_02520"
     CDS             complement(7070..>7312)
                     /locus_tag="PKI68_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52599.1"
                     /translation="DEIRQTIVEYAKKEGLQAVIDNSARKAAIGVFIYTHPDLEITDI
                     ILTVLNSKRPDIPDEGNLLNDGLNSTNKTVKDSSGS"
     assembly_gap    7313..7412
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7472..9844)
                     /gene="bamA"
                     /locus_tag="PKI68_02525"
     CDS             complement(7472..9844)
                     /gene="bamA"
                     /locus_tag="PKI68_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNX52600.1"
                     /translation="MKNMKLLGVLMLMSGLAASGFSALVLSEIRIENVEKAQLDESFV
                     KAYTSLRAGQEIASEVELNAAVAHDVDNLRRSERFSYVRAVVEQDNGRYTLVYKVASR
                     LRLRQIDITGADSIGNRKIKKELELELGSYVDQALVGEKVRKVEEYCRKNKYPDASIA
                     FELKADEKNSTADLLLKVSEGEKLRVKKISFDGGLVANDSFCKRITPNIFPSRKNADH
                     YNTWELTKVMQQKKTWWITPWFGAYKPEMKEADLAALRKFYQDRGFLDVKIEGPELKH
                     LGDGKLELVYHVQESVKYQVGSISFDGNTLFETAELEKQLKIQSGALASRASIDDTAA
                     AVNRYYGNRGYIRNDVQPVINTDSATKTASIRFNVHEGSLANINEIDIRGNEKTRDEV
                     MRRELAVYPGELFHQGKVETSESRLKNLGYFETVSSSYTPAETGTNSYDLAFKVKEKA
                     MGSFLIGAGFSSVDDLVGFAELSHGNFDIQRWPPVGDGQKVKVRVQAGTKRSDLEVSF
                     IEPWFMDRKLALGQDLYYRDASYYSDQFDLQTLGSKTSLTKPVFNPFTRATVSYSLEQ
                     FNVDNIDAPTNAFVRGDEGATLKSTVGTSISRDTRDQFFIPTKGNFSSAGAEVSGGPL
                     AGDESIYMLEAKSSQFWPVLDDHVFNLKGEVRTVDSYGSSDHVPIFDRLFLGGPRTIR
                     GFKYRDVSPRDPADNDEPIGGLSSWYATAEYTVPLWTKIRAAVFYDIGAVSENSFDIF
                     GADINSSYGIGARIDLPMFPLRLDYAIPHIADENNSGSGGRFSFLLGYSF"
     gene            complement(9850..11229)
                     /gene="dnaB"
                     /locus_tag="PKI68_02530"
     CDS             complement(9850..11229)
                     /gene="dnaB"
                     /locus_tag="PKI68_02530"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNX52601.1"
                     /translation="MSSIDQSGRRLPPYSEEAERGVLGSALIESNRVIELCSIKFLSV
                     DSFYVPAHRLLFEQLMGMYQGARAIDLLTVGEDLKKNGRLEQVGGYAFLESLIDSTPT
                     SAHAEFYIDIVQQKHLLRRIISQAAEAIDHCYTDEDAEELLSRTEEAFFELSDKKTSS
                     SEGWKDVVYKTAGQIIEGNTLSNGVPTGFIDLDKELRGLHNTDMIVLAARPSMGKTSL
                     AMNIVEHAALGRGGGKKHAVGVFSLEMSQEQLVRRMLYSRAGVPAWKDFFSESDRTKL
                     SSAADQLGKASIIVDDSAGLDVMDLRARARRMKRIYNIDLIVIDYLQLLADKRRSREG
                     RQQETASISNSIKAMAKELKVPVLILSQLSRAPEQGTRDGKPRLSDLRDSGAIEQDAD
                     VVLLLQRPSYYNKELEAGSGEDTLAIVDIAKHRNGPTGEVKLNFFNDITRFEDRDTRH
                     GVDGAFPAE"
     gene            complement(11226..11675)
                     /gene="rplI"
                     /locus_tag="PKI68_02535"
     CDS             complement(11226..11675)
                     /gene="rplI"
                     /locus_tag="PKI68_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNX52602.1"
                     /translation="MAVEVLLMDAVPGLGIEGDVVRVADGYARNYLFPNGLASVVTDG
                     KKRQIEKKRIERLELMRKEKAAAEELAKKFESISCTIAVKTSEGGKMFGSVGAAQVIE
                     KLAEQGLTLEREQLKLEAPLHELGVFDVTVTLHPEVKTVLKVWIVEE"
     gene            complement(11700..11909)
                     /gene="rpsR"
                     /locus_tag="PKI68_02540"
     CDS             complement(11700..11909)
                     /gene="rpsR"
                     /locus_tag="PKI68_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNX52603.1"
                     /translation="MAYVANKEEKGGRLLEGVTEIDYKDAELLRKFMTERGKILSRRY
                     TGVTSKQQRQVKRAVRQARVMGLLR"
     gene            complement(11929..12357)
                     /locus_tag="PKI68_02545"
     CDS             complement(11929..12357)
                     /locus_tag="PKI68_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNX52604.1"
                     /translation="MSTLNRVFLMGNLTRDPEVRYTPSGTAVGDLGLAINENFKNKAG
                     ETVESTVFVDVEVWARQAETCAEYLYKGSPVFVEGRLKLDQWTNQQGEKRSKLRVRAD
                     RVQFLGAPKRGADAADAPQNSMPPPADFEAPAADEDDIPF"
     gene            complement(12361..12672)
                     /gene="rpsF"
                     /locus_tag="PKI68_02550"
     CDS             complement(12361..12672)
                     /gene="rpsF"
                     /locus_tag="PKI68_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNX52605.1"
                     /translation="MKTLYEGLFIFPETLEEAQLDLSIEAVKTELEKLGGTLESSTRL
                     GKRSFARPLRKKKAGFYVVIMFRLEGEQINAFKHRLKLATDVFRAQFMQKDGVVAAQE
                     A"
     gene            complement(12690..13256)
                     /gene="pth"
                     /locus_tag="PKI68_02555"
     CDS             complement(12690..13256)
                     /gene="pth"
                     /locus_tag="PKI68_02555"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNX52606.1"
                     /translation="MKVIVGLGNPGREYEMTRHNIGFLVLNELARRSNVSFRKSWWSP
                     VETAKGRIGNETVRLVKPQTFMNRSGQVVGPALRKAGGQPEDMLIVFDDVALGWGQLR
                     VRAQGSAGGHNGVQSVMDVLGDGAFGRIRVGIGPKPDSLSLSDYVLGPFSDAERRSLD
                     AVVRRAADAVEMVCAAGIEQTMNCFNRG"
     gene            complement(13282..13929)
                     /locus_tag="PKI68_02560"
     CDS             complement(13282..13929)
                     /locus_tag="PKI68_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNX52607.1"
                     /translation="MDAKITVKSRDVKGSASARRLRRDGWVPGVIYSEGGVARMVSIP
                     KHEFEQMLRHHASEHVMVKIQIEGGAEESVLLKEVQHDALSGGVEHVDMQKVDMNKKL
                     RVEVPVELVGEADGVRNQGGVLDHLLHYLEIECFPADIPEEIDVDVTDLKLGDILTVK
                     DIKIDGSKYTILMDADVGVAAVAAPKVAEEPVAEEAAAAAGPEVIREKKPEEETK"
     gene            complement(13943..14872)
                     /locus_tag="PKI68_02565"
     CDS             complement(13943..14872)
                     /locus_tag="PKI68_02565"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNX52608.1"
                     /translation="MKILSGTAHRDLAERIAAKAGEKLCDVQISRFPDGEIFVKIAEN
                     VRGRDVFIIQPTSYPPNENLMELLIMIDAAKRASASRITAVMPFYGYARQDRKDQPRV
                     PITAKLVANLLVAAGTNRLLTMDLHAQQIQGFFDIPVDHLYAAPVFVKYLREQKLDNL
                     VVVAPDTGGMKLAAAYADMLDAGLAVVGKERKSAERVEATHLVGDVKGCNAVLVDDMT
                     STAGTLTAAAKLLERAGAASIRAAVSHSLLNCKGVERLNDSPIIELVTTDSIPARCDT
                     GSKVKVLSVAELLAEAICRIHDNNSVTSLFRIT"
     gene            complement(14935..15005)
                     /locus_tag="PKI68_02570"
     tRNA            complement(14935..15005)
                     /locus_tag="PKI68_02570"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14971..14973),aa:Gln,seq:ttg)
     gene            15199..16707
                     /locus_tag="PKI68_02575"
     CDS             15199..16707
                     /locus_tag="PKI68_02575"
                     /inference="COORDINATES: protein motif:HMM:NF016252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HNX52609.1"
                     /translation="MNKYSLAPVVLLCAAFNGIAQDAGSRFDFNALKNRAQNLAAAPY
                     QAPDRSLPEELSRFNYDDMRNIRFDPKAAIWRMERLPFQMQFTHRGGLQNSHITMNYI
                     EDGQVEPIPFSKNLFNYSQVKINGKLPEDLGFYGFRIHYPLNSMEYLDEVISFQGASY
                     FRALGKGLHYGLSARGLALNVAGQTPEEFPDFIEYWIEKPDTRATRIKIFALLNSPSL
                     AGAYEMTLIPGSDTVMEIKTALFARKPVEGLGLAPLTSMFWFGEHSTSRHGDFRPEVH
                     DSDGLLVHTAADEWLWRPLSNEGLVKTSCFANDNPRGFGLFQRDRDFSHYEDLETFYH
                     NRPSAWVEPGPGWGQGELRLVELPSGTEYADNIVAFWMPQEQLTPEHPLEYSYRLHWF
                     RDDATLPPLAQALATRIGNATEYPGSKKFVLDFSNAPGLDRNNPDSVTPVITLSNGTL
                     KGKHLQRNDLGNAWRVFFDVEPIDRTKPVDMRCFLQSGGLPRSETWTYFWNP"
     gene            16683..17279
                     /locus_tag="PKI68_02580"
     CDS             16683..17279
                     /locus_tag="PKI68_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52610.1"
                     /translation="MDLLLEPIETFVPTWGSPEEWNSAYEKLESYLRAHEVDSHLHRA
                     HITTMILRRVSRRWQGQTPPEPLATLAIEEANRLLNEWFSLIMDLPAGANADFTAADG
                     RVALFLCDGSLRWPYAFLTPHVPNELKTAMRSSLMRAGPELQISNMVPRPIDLGLIPE
                     FADDAMDRLNRSPIIKALIGWLIFLAILTYLFWRTRQP"
     gene            17276..>19326
                     /gene="mdoH"
                     /locus_tag="PKI68_02585"
     CDS             17276..>19326
                     /gene="mdoH"
                     /locus_tag="PKI68_02585"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HNX52611.1"
                     /translation="MIPVKLLSSAQKVRRRILFFSLVLLLTTLASWVMADILWRGGMN
                     SCEIAILILFTVLFLPVSLGVVQALTGFFILWRRRDASRQLSLLEKMDPPVELPVTAV
                     VLPIFNEDVSRVYEGLRAIYRSVEKTGNLANFDFFILSDSNDPNKWIEEEMAWAELCK
                     QVHGFGRIFYRKRKIALNRKSGNISDFCRRWGSKYRYMVILDADSVMSGDCLVKLAQL
                     MELQPQAGIIQAAPVQSMSESFFGRVMQFAGTLYGPIFQAGLNFWQADEGNYWGHNAI
                     IRLAPFIEHCALPDLPASKTPRAKFMSHDYVEAALMRRAGYEVWLAYGLGGSYEGGPP
                     TLTDMAKRDRRWCRGNFQHSWLMLSPHVRPINRLHLALGIMSYLSSPLWFFFMLLGTM
                     QFVLASRFTQRTFDADVGLSKVLDIGGNRLAIILFATTISLLFLPKLLGLLLILRHKQ
                     EAKPFGGRLRATVSVVLEHLTSMLITPVLMLFHSRFVLGVMAGQEVGWGSQRRGGDDG
                     IEWGEAIEAHWGHTIIGAVWGILAWWINPAFFWWMSPIFIGLVCSIPFSVLLSKTSVG
                     AALRRTGLFMTPQESCPPVELAELQTNLDACQQHIPPLEPLRKNYGFMQVVIDPYVNA
                     IHISLLRRRNKHRRLDSAYFTQLQNRVVTEGPDVLSRREQMALLLNPECMAWLHEQIW
                     L"
BASE COUNT         4255 a         5684 c         4810 g         4477 t
ORIGIN      
        1 ctgcgccggc agacatttca ggatattgag ctgttcgctg tagattccgg atcgaccgac
       61 ggctctctgg atgcgctgcg tgccgctgtg gatcctgcac acctggtttg cattcctccg
      121 aaagcttacg ttccgggcgt ggtcctcaac gacatgaccg cccgcacgac gcaggagttt
      181 atcgtcttcc aaaatgccga tgcaattttc caaagcgacg acgctctcga aaaactgctt
      241 cagccgcttt tctccggcga agccgatgcg gtcatgagcg ctcaggtaac gcgccctgac
      301 gcccgcttta ttgtgacata cgactacctg cgagcctaca acccgaaaaa catcaagggc
      361 gataacgtcg actttttttc cgccgtcacc tgcgccttcc ggcgcgatct gtgggagaaa
      421 attaaaatcc ccaacatcgg ctatgcagag gatgtcgcct gggcaaaact ctgccggacg
      481 cagggcgccc ggttcaaact ggtaacagac tccgtggtcg agcactccca caactacacg
      541 ctcaaaggac tttaccggaa aaaattccgg catggtgtga cctttgcaca cacttacgga
      601 cgccgcacaa acctgcttct tcagacgctg gacctctgca aagaatggat ccgggatctt
      661 ttctacgccg ttcgtaaagg gcggcttgat acgattccct acaacattgc ctaccgtgcg
      721 gtcatccaca ccggccttta ccgcggtctg aaagaaggcg cccgtgggta aatactctgt
      781 caaaggctcg ccggcatttg atgcgcgcat tgacgcggat atgaaacgga ttgccgacgc
      841 ggtgtatgcg tcacattttt ccagacactg gaaagcgctg gttctgctgg gcggctacgg
      901 gcgcggcgaa ggttcaccga tgatcggccc gaacggcgaa gagcttccct tcaatgacta
      961 tgatctgatc gtcgtgacgg attcgctcga tccgctgctg aagcgcgcgc tgaaaaaact
     1021 ggagaaagag ctttcggaac aagtcgggct tccggtcgat ctgtacccct atctcagctc
     1081 ggcgctgccc aaatgcgagt tttctctgct caactatgag ctgcggtacg gacaccgcgt
     1141 aatccgcgga gataaaaaaa ttcttgatgc catgccggcc tacccgcacg atgccattcc
     1201 tctttcggaa ggcacacgcc tgctgatgaa ccgcggcaaa ctgctgctcg acatcaagcg
     1261 ccgccttgcc cggccggaca cgctgaccca cgaagaacgc cagcggtttt taaaatttct
     1321 gtttaaagcc gatctcgctt tcggcgactg cgccctgctg ctgcgcggag cctatgacct
     1381 ttcgtatgtc gtcaaaaggc accgcctgcc ggaaaccgat ctgtccgggc tggagaattc
     1441 gcgcgatttt gtcagcgcct acagccgcgc aattgacttc aaggaacggg cggatttcaa
     1501 accgctggac aacagcaaca tccatcttca gtttgatgaa acagttcgcc gttatgaata
     1561 cttttttctt tggtatgaac agcggcggct caaccggaaa ttccacactc tggggaaata
     1621 cgcacgcact ttcccgctcc tcgggaatga aggctctccg gtaaaaaatg ccgtgcataa
     1681 tctgcgcgct ttcgggccgg gcgcacttcc gtactgcttt gcacacccgc gactgcggct
     1741 ttacgccgcc cttccgctgc tgctgacaaa acaggcggac cgtgaagaaa tccgctggct
     1801 gctgaattcc cgcgaaagcg gcacggaaga gctgtacaaa tccttctatg cgcttcagca
     1861 gaggttttca tgaaaaaaca gaaactgagt ctgtttattt tcatcgacgc gttcggacgg
     1921 gagattttcc tccgtcatcc gtttttcctt cagggactga ttcgcgacca caagaagctc
     1981 gacacgattc tgggttattc atcggcatgc gatccgtcga tcatctccgg aaaactgcca
     2041 tgtgaacacc tgatgtggtc gtcgttttat tacgatccga aaggttcgcc gtttaaatgg
     2101 acaaaggcgc tggccattct gcccgacgcc gttttccggc gcggacgggt gcggcactat
     2161 ttaagccggc tgattaaaaa ggtgctgggc ttcaccggct acttccagct ttacggcgta
     2221 ccgttcaaat atcttccgct ttttaactac gccgaaaaaa aacggatttg ggagccggga
     2281 ggactgctgc gcggcgaaac gatttttgat gaactgacgc gacgcggaat tcctcattat
     2341 gtccatgaca gcgccgtcag cgacgaaacc aaactggccc ggctgaccgc agatatcaag
     2401 tcgcaacgga ttgagttcgc ctactgctcg ctagggaaac ttgatgcact gatgcattcc
     2461 gtcggcacaa aagatcagaa ggtgacagag ctggttcact ggtacgacca gcaggtccgc
     2521 aaactgattg cggcggcgga cgaacagtat gacgaggtcg cctggtatgt ctttactgac
     2581 cacggtatgc atgacatcac ggaaggatac gacctgattt ccgatattga aaaaaccggc
     2641 ctgcgctgga accgggatta cgtcgcattc tacgattcta caatggcgcg cttctggttt
     2701 ctcaatgaaa ccgcccggca aaagatcacc gccctgctgg gcagccaccc gaaaggccgg
     2761 attctggatg atgaagagct gaaacgtgaa ggagttttct tccctgacgg aatgtacggg
     2821 gaactgattt tcctgataaa ttccggcatt caaatcgttc ccggcttcat gggcgttaag
     2881 cagatcaaag gaatgcacgg ctatcaccct caggatgcag attccgcggc atccatctgc
     2941 tctaaccgtg aactgccggc gggcttgaca aagatacatc aaatctatca tctaatgcac
     3001 aaggagctcg gctttagtga gtgataaacc ccaagagttg tccgcaaagg aaaaagacgc
     3061 cgtcctgatt gtcgacgacg atgcgttcat gctgaaggtg ctctaccggg tgcttcagaa
     3121 ggaatatgcg ctttatcagg caggatcggc agaagaagcc atcgaggtcc tgcgcggacg
     3181 gcatgttaaa gccatcctct gcgaccatgt tctgccgggc caaagcggac tggattttct
     3241 gattacgctc aaacaacgca actctcagat caaaagcgta cttttcagcg cggctcttga
     3301 aactgaacag ttcatcaagg cgatcaatga gggtcaggtt ttccgcttcg ttaagaagcc
     3361 ggcttcaccg gccgatatcc agcaggccgt ccgcgaggcg gttcgtcagt atgatatcga
     3421 atccctgcag cagcagctgc tcatcaacca gaaggatatt gataagcagg tttacagcgc
     3481 accctactgg ctctaccgcc tgcgcgtgat cgcccagcag tccttccgtt acggcgcacc
     3541 gcttttcggc aatattctgg cgctgatgct cgcactgggc gtgatcgccc tgagcgtcgg
     3601 catctgcatc atgctcatca tctattttgt caaaagctat ctgggaatcg acatcttcca
     3661 gggacatctg ctgcactaga agaattggtt tcaccccggc tttacgggca aaagatgaaa
     3721 ataaaaagac tcccttttca gggagccttt tccgttttgc agttcggttt tgattacatc
     3781 atgaacagca tttcggcata ggtcggaacc ggccagatgt cgtcgggaac catcttttcg
     3841 agcgcatcga ccgaagcgcg aactttgccc atcgccgcaa tcagcttctc agcaccgtgc
     3901 gctttttcga gcgctgcaac ggctttgtaa agcttgtcgg tttctgagca catcgctttg
     3961 agctcggcct tggcgccggt caccgcacag cctgtgacgg ctttgatcga tgcagccagc
     4021 tcagcctgcg cggttacagc agccggaata atcatggttt tcgccatggt cagcgctaca
     4081 cccgcttcaa tctcgatggt cttttcgtag gtttcgttgt agatgtcgaa acgggagtgc
     4141 agttcttttt cggagagcac accgtatttg ctgaacagct tcttcgcttt ttctgtattg
     4201 aaagctttca gcgcatccgg cgtctttttc acgttcggca gaccgcgtct ggcggcttcc
     4261 ttcgcccagg cttcggtgta gttgtcgccg ttaaacagaa tgcgtttgtg tttcttgatg
     4321 agaccggcaa gaagcttctg cagggattca ttgaagtcct tgcctgcttt cacgtccgct
     4381 tccagtttcg tgcagatttc gtcgagcgct tccgccacga tcgtgttcag ggcaacgtta
     4441 gaaccggcgc agctctggtt ggagccgacc gcgcggaatt caaacttatt gccggtgaat
     4501 gcgaacggtg acgtgcggtt acggtccgtg ttaccgcgag gaagcgccgg cagcgtgtca
     4561 acgcccaggt gaatgtaccc gccggtctta gagctcttcg ctccgccttt ttcgatctgc
     4621 tcgacaatgt ccgtgagctg atcgccaagg aagatggaaa tgatcgccgg gggagcttcg
     4681 ttggcgccca ggcggtggtc gttacccgcc gtggcaaccg tggcgcgcag aaggtcggca
     4741 tgtgtatcaa ccgctttcat cagcgcgcag atcatggtca tgaacttggc attttcatgc
     4801 gggttatcgc cgggagagag ccagttcttg ccgtccggac cgcaaagcga ccagttgtta
     4861 tgcttgccgg aaccgttgac gccagcgaag ggtttttcgt gcagcaggca gacgaacccg
     4921 tgtttttccg ccgtcttgcg catcacttcc atggcaatca tgttgtggtc aaccgcaaga
     4981 ttgagttctt caaacaccgg tgccatttcg aattgcgccg gggccacttc gttatgacgg
     5041 gttttagacg gaacacccat tctccaaagt tcagcatcca ggtcttccat gaaagccagc
     5101 acacgggttt taatggcgcc aaagtagtgg tcttccatct gctggtgctt ggcaggcttg
     5161 ctgccgaaaa gtgtacggcc ggtctgacgc aggtcgaggc gggcattgta gagctcctcg
     5221 tcgatcagga aatattcctg ttcggcgccc agtgtcgcgt aggctttctg tttgatctgg
     5281 atgccaaaga gcttcgccag acgttcagcc tgcgtgctga gtactttcat ggaacgcaac
     5341 agaggagtct tcttgtcgag tgcttcgcca tgataaccgc agaaaacggt cggaatacaa
     5401 agagtgttgt ctttgatgaa ggccgggctg ctcggatccc agccggtata accgcgggct
     5461 tcgaatgtag cgcgcagacc gccggacggg aagctggaag catcaggctc gccctgaatc
     5521 agttcagcgc cggagaattt gcatacaaca gaaccttcgc cgtccggaac aataaaggag
     5581 tcatgttttt cagcagtcga gccggtcagc ggctggaacc agtgcgtata gtgagttgca
     5641 ccttttgaaa cagcccattc tttcatggct tcggcaactt cgtccgcgat accggcatcc
     5701 agactcttgc cggttttaat agtcgctaca agcgatttaa cagtcgcctc agacaggtgt
     5761 tcacgcatga ctttcatgct gaatacattt tcaccaaaca gatcggtcgg attagccatt
     5821 tcatactcct ttatactgtt gaataaatat tcattcccgc gtaaccagcg aaaacatttt
     5881 attgtaagca aaaaccattc ccaaacagac catcgaataa aaataggcgc aaggcgcttg
     5941 ctataacatg gttgcagata aaatttcccg gactgaaaat ttgcagctca ccgtatcaaa
     6001 acacatttgt aactatttaa attttaaaaa cacaaatatg tcactcactc gctttgctcg
     6061 agattgcgta ttctgagtgc gagctcggcc acacgttctt tcagtttcgg aagcagcatg
     6121 attcctgcct gggttctttt aaatttggca gcgggcatcg ccgggacacc aatcatatac
     6181 tcgccggcgg ccacatcctt ggtcaccgct gcttttgcgc caatcacagc tccggctccg
     6241 atgtgcacat gcccggcaac gccggcctgt ccggcaataa taactttctc atcgatatgc
     6301 gtactccccg caatggcaac ttgcgcaaca agaacggagt gatcgccgat aacgacgtta
     6361 tgtgcaatct gcaccagatt atcgatttta acgccgtgtc cgatacgggt cttgccaaac
     6421 cgggcacgat ctatcgtcac attcgccccg atttcgacat catcaccgat ttcgacaatt
     6481 ccgatctgcg aaatttttgt acgaatgccc tgcccgtcaa ccgagtaacc gaatccgtca
     6541 ctgccgataa cggttccgtt gtgaacgatg actctttttc caaggatcac cgcttcacgc
     6601 agagacactt gaggataaag ccagcaattc tcacccagca ccgctttgcg accgatgtaa
     6661 cactgcggaa aaacaactgt gcctgcaccg attttcgcgc cggactcaac taaacaaaaa
     6721 gcaccaatgg aaacgttttc accgatctcc gcatccgccg ccaccgaagc cgacggatgc
     6781 actccgattg ccgggcgggg cggcggtgta taaaaaagca tagcggcttt tgcaaaagtc
     6841 tcatcaggat tttttgcgcg gattaaagca gctgaacagg cgctgctcca gttttctgac
     6901 acaataacag cagaagcttt tgtcgcagcg gccgcggatg catagcgctg atttgcgata
     6961 aaactgattt caccttcgac cgcttcgccg ataccggcta ccccgcgaat atcgatattg
     7021 ccgtcgccgt cgactatacc accggttttt tcagccactt ccgagagttt catgatccgc
     7081 tgctatcctt cacagtttta ttggttgagt tcagtccatc attaagcaga ttcccttcat
     7141 ccggaatatc aggacgttta ctgttaagaa ctgtaagaat gatgtccgta atttccaggt
     7201 cgggatgggt ataaataaaa actccaatgg ccgctttgcg cgcggaatta tcgataaccg
     7261 cctgaaggcc ttcttttttc gcgtattcca cgatggtctg ccggatttca tcnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngccaacag caaaacagaa aggcctgcca
     7441 gtaataattt tttcatatta atacccttcg atcagaatga atatcccagc agaaagctga
     7501 accgcccgcc ggacccgctg ttgttttcat cggcaatatg cggaatcgca tagtccaaac
     7561 gcagcgggaa catgggcaga tcaatgcggg cgccgatacc ataactggaa tttatatcgg
     7621 ccccgaaaat atcgaaagag ttttccgaga cggccccgat atcataaaag accgccgcac
     7681 ggattttggt ccaaagaggc acggtgtatt cagcggtagc gtaccagctg ctcagaccgc
     7741 cgatgggctc gtcattatca gccgggtcgc gcgggctgac atcgcgatat ttaaacccgc
     7801 gaatggttcg cggaccgccg agaaacagac ggtcgaaaat cggcacatgg tccgagctgc
     7861 cataactgtc gaccgtgcgc acttcgcctt tgaggttgaa cacgtgatca tccagcaccg
     7921 gccagaattg ggatgatttt gcctccagca tgtagatgct ctcatcaccg gcaagcggtc
     7981 cgccggaaac ttccgcaccg gccgaactga agttcccttt ggtcggaatg aaaaactggt
     8041 cgcgcgtatc gcgggaaata ctggttccaa cggttgactt aagcgtagct ccttcgtcgc
     8101 cccggacaaa ggcgttcgtc ggggcatcga tattatcgac attgaactgc tccagcgaat
     8161 agctcaccgt agcgcgcgtg aacggattga aaaccggttt ggtaagcgat gttttgctgc
     8221 ctagcgtctg cagatcgaac tggtcgctgt aataactggc atcgcgataa tacaaatcct
     8281 gacccaaggc cagcttgcga tccatgaacc acggttcaat aaaagaaact tccaggtcgc
     8341 tgcgcttggt acccgcctgc acacgcactt tcactttctg tccgtcaccg accggcggcc
     8401 agcgctgaat gtcaaaattg ccgtgcgaaa gttccgcgaa cccgacgagg tcgtcgacac
     8461 tggaaaagcc cgcgccgatc aggaagctgc ccattgcctt ttctttcacc ttgaaagcga
     8521 ggtcatagct attggttcct gtttccgccg gcgtgtatga actgctgact gtttcgaaat
     8581 aaccgagatt tttgagacgg ctttcactgg tttcgacttt gccctgatga aacagctcgc
     8641 cgggataaac agccagttct cgccgcatca cttcatcgcg ggttttttcg ttcccgcgga
     8701 tatcaatttc gttgatgttt gccagcgagc cttcatgaac attgaaacgg atgctggctg
     8761 tttttgttgc cgaatcggtg ttgattaccg gttgcacgtc gttacggata tacccgcggt
     8821 tgccgtaata gcggttcaca gcggccgctg tatcgtcgat agatgcacgc gaagccagcg
     8881 cgccggattg gatttttaac tgcttttcga gttcagcggt ttcaaacagg gtgtttccgt
     8941 caaagctgat cgagccgacc tgatacttga cactctcctg tacatgataa accagctcca
     9001 gctttccatc gccaagatgc ttgagttccg ggccttctat tttcacatcc agaaacccgc
     9061 ggtcctgata aaatttgcgc agagccgcca ggtcggcctc tttcatttcc ggtttatacg
     9121 caccgaacca cggggtgatc caccaggtct ttttctgctg catgaccttg gtcagttccc
     9181 atgtattata atggtcggca tttttccggg acgggaaaat attcggtgtt atccgtttgc
     9241 agaagctgtc atttgcaaca agcccgccat cgaaactgat cttcttcact cgcagctttt
     9301 cgccttcact cacttttaac agaagatcgg ctgtgctgtt tttttcatcg gctttgagtt
     9361 caaaagcgat cgaggcatcg ggatatttat ttttgcggca gtactcttcg actttgcgca
     9421 ccttttcacc caccaaagcc tgatccacgt aactgcccag ttccaattcc agctcttttt
     9481 tgatcttgcg gttaccaata ctgtccgcgc cggtaatatc aatctggcgc aggcgtaacc
     9541 ggctggctac tttataaacc agtgtgtacc tgccattgtc ctgctcaacc acggcacgca
     9601 catacgaaaa ccgttcagac cggcgcaagt tatcaacgtc atgggcgacc gccgcattca
     9661 actccacttc gctggcaatc tcctgcccgg cacgcaaaga ggtatacgcc ttcacaaaag
     9721 actcatccag ctgggctttt tcaacatttt cgatgcggat ttcgctgagc accagtgcgg
     9781 aaaatccgga tgcggccaat ccggacatca gcatcagcac acccaaaagt ttcatatttt
     9841 tcatattttt cactccgccg gaaaggcgcc gtcaaccccg tgccgcgtat cgcgatcttc
     9901 aaaccgcgta atgtcattga aaaagttcag cttcacttca ccggtcggtc cgttacgatg
     9961 cttggcaata tccacgatcg caagcgtatc ctccccggaa ccggcctcca gttctttatt
    10021 ataataagac gggcgctgga gcaacagtac gacgtcggca tcctgctcga ttgcaccgga
    10081 atcgcgcagg tcggaaagcc gcggcttccc atcgcgggtc ccctgttccg gagcacggct
    10141 gagctgactc agaataagca caggaacttt cagctccttg gccattgctt tgatgctgtt
    10201 tgagatagag gcggtttcct gctgacggcc ttcgcgcgaa cggcgcttat cggccagcaa
    10261 ctgcagatag tcgatcacaa tcagatcgat attataaatc cgtttcatcc ggcgggcgcg
    10321 ggcgcgtaaa tccatcacgt ccaaaccggc cgagtcgtcg acgataattg aagctttccc
    10381 caactgatcg gccgctgacg aaagtttggt gcggtcggac tcggaaaaga aatctttcca
    10441 cgcaggaaca ccggcccggc tgtaaagcat acggcgaacc agctgttcct gtgacatttc
    10501 aagactgaac acgccgaccg catgcttctt cccgccgccg cgcccgagcg cggcgtgctc
    10561 cacgatattc atggccagcg aggtttttcc catcgaaggc cgcgccgcca gcacgatcat
    10621 gtcggtgtta tgcagtccgc gcaactcttt atccagatcg ataaacccgg tcgggacacc
    10681 gttggaaagc gtattgcctt cgataatctg gccggcggtt ttataaacga catctttcca
    10741 gccttcggaa gaagacgtct tcttatcgct taattcgaaa aaggcctctt cagtgcggct
    10801 cagcagctct tccgcatctt catccgtata gcaatggtca attgcttccg cagcctgact
    10861 gataatccgc cgaagcagat gcttctgctg aacaatatca atgtagaatt cggcatgcgc
    10921 tgaagtcggc gtgctgtcga tcagactttc aagaaaagca taaccgccga cctgctccag
    10981 ccggccgttt tttttaagat cttctccgac cgtcagcagg tcgatcgccc gcgccccctg
    11041 atacattccc atgagctgct caaagagcag ccggtgcgcg ggaacataaa aggaatcgac
    11101 tgaaagaaat ttgattgaac agagttctat aacccggttg gattcgatca gagcagagcc
    11161 aagaacgccg cgttcggctt cctcactgta gggcggaaga cgacgcccgc tctgatcgat
    11221 agaactcatt cttcaacgat ccataccttc agcacggttt ttacttcggg gtggagagta
    11281 acggtaacgt cgaatacacc gagctcatgc agcggagctt cgagtttgag ctgctcacgt
    11341 tcaagagtaa gcccctgttc ggcaagcttc tcgataacct gagcggcgcc gactgagccg
    11401 aacatttttc cgccttcgga cgttttaaca gcgatcgtgc aggagatgct ctcgaatttt
    11461 ttcgccagct cttcagccgc cgctttttct ttacgcatca gttcaaggcg ctcgatgcgc
    11521 ttcttttcaa tctgccgttt tttgccgtcg gtcacaaccg aggccagtcc gttagggaaa
    11581 aggtagttcc gggcatagcc gtcagctaca cgcaccacat cgccttcgat cccgagtccc
    11641 ggcaccgcat ccatcaataa aacttctaca gccatttctg attctcctct gtttgcgctt
    11701 tagcgcaaaa gtcccattac acgcgcctgg cggaccgccc gcttaacctg acgctgctgc
    11761 ttggatgtta caccggtata acggcgcgaa agaattttgc cgcgttcggt cataaattta
    11821 cggagcagtt ccgcgtcctt gtagtcaatt tcggtcacgc cttctaaaag ccggccgcct
    11881 ttttcctctt tattcgccac atatgccatg atctataccc tttccttatc aaaacggaat
    11941 atcgtcttca tccgcagccg gagcttcaaa gtctgccggc ggcggcatgc tgttctgggg
    12001 cgcatcagcg gcatcagctc cgcgcttggg cgctccgaga aactgaaccc ggtccgcgcg
    12061 aacccgcagc ttactgcgct tttccccctg ctgattcgtc cattgatcga gcttcaagcg
    12121 gccttcaaca aaaacaggcg accccttata gaggtactcc gcgcaggttt cagcctgacg
    12181 ggcccacact tcaacatcca caaacaccgt gctttcaact gtttcgccgg ctttgttttt
    12241 gaagttttca ttgattgcca gtcccagatc gccgaccgct gttcccgacg gcgtataacg
    12301 cacttcggga tcgcgagtca gattgcccat caggaacact cgattcaagg tcgacatgtt
    12361 ttatgcctcc tgcgccgcaa ccactccgtc tttttgcata aactgtgcgc ggaacacatc
    12421 ggtggcaagt ttcaggcggt gcttgaacgc attgatctgc tcgccttcca gacggaacat
    12481 gatcaccacg taaaacccgg cttttttctt tctgagcgga cgcgcaaacg accgttttcc
    12541 aagccgtgtc gaactttcca gtgtaccgcc cagcttttcc agctcggtct ttacagcttc
    12601 aatcgacagg tcaagctgcg cttcttccag cgtttccggg aaaataaaaa gcccttcata
    12661 caatgtcttc aatttctctc tccttgctct tatcctctat taaagcagtt catggtctgc
    12721 tcaatccctg cggcacagac catctccacc gcatccgcgg cacggcgaac cacagcatcc
    12781 agactccgcc gctcggcgtc cgaaaacggt ccgagaacat aatctgagag cgacagacta
    12841 tcaggtttcg ggccgatgcc aacccgaatt ctaccgaaag cgccgtctcc taatacgtcc
    12901 atcaccgact gcactccgtt gtgtccgccc gccgaacctt gagcccgaac gcgcaactga
    12961 ccccaaccaa gcgccacatc atcaaaaaca atcagcatat cttccggctg cccgcccgcc
    13021 ttgcgaagag ccggcccgac aacctgtccg ctgcggttca taaacgtttg cggcttcacc
    13081 agccgcaccg tttcattccc gatccggcct ttagccgttt ctacgggtga ccaccagctc
    13141 ttgcgaaaag aaacgttcga gcgacgggca agttcgttca gcaccagaaa cccgatgttg
    13201 tggcgggtca tttcatattc ccgcccggga tttccaagcc cgacaataac cttcactcaa
    13261 acagtctccg cgaaagcgtg tttattttgt ttcttcttcc ggcttcttct cgcggatcac
    13321 ttcaggcccg gcagcagccg cagcttcttc agcaaccggt tcttcggcaa ccttcggcgc
    13381 agcaaccgca gcaacaccaa catccgcatc catcaaaatc gtgtacttgg agccatcgat
    13441 cttgatgtcc ttaaccgtaa gaatatcgcc gagcttcaga tcggtgacgt ccacatcaat
    13501 ttcttccgga atatcggccg ggaaacattc gatttcgagg tagtgcagca gatgatccag
    13561 cacaccgccc tgatttctga cgccgtcggc ttcaccaacg agctcaaccg gaacttcgac
    13621 gcgcagcttc ttgttcatgt cgaccttctg catgtcaacg tgctctacac cgccggaaag
    13681 cgcatcgtgc tgaacttctt tcagcaatac agattcttcc gcaccgcctt caatctgaat
    13741 cttaaccatc acatgctcgc tggcatgatg gcgcagcatc tgctcgaatt catgtttcgg
    13801 aatcgaaacc atgcgggcga caccgccttc gctgtaaatt acgcccggca cccagccgtc
    13861 gcggcgcaga cggcgcgcac tggctgaacc ttttacatct ctgctcttta ctgtgatctt
    13921 tgcgtccatt gctcttttct cctcaagtta ttctgaaaag tgatgtcacc gaattgttgt
    13981 catggatacg gcagattgct tcggccagca actccgctac tgaaagtact ttcaccttgc
    14041 tgccggtatc acaccgggcc ggtatgctat cggtcgtcac cagctcgata atcggcgaat
    14101 cattcaagcg ttcaacgcct ttgcagttca gcaacgaatg gcttaccgcg gcgcggatag
    14161 aggccgcgcc ggcacgctcc agcagtttgg cggcggccgt cagcgtaccg gcggttgacg
    14221 tcatgtcgtc aaccagcacg gcattacacc cttttacatc gcccaccaaa tgggtcgcct
    14281 cgacccgttc ggcgctcttg cgctctttac caaccacagc gagacccgca tccagcatat
    14341 cggcatacgc cgcagccagc ttcatcccgc ccgtatccgg agccacgact acaaggttgt
    14401 ccagcttctg ctcacgcaga tatttcacaa aaaccggcgc ggcataaaga tgatccaccg
    14461 gtatatcaaa aaacccctga atctgctgcg catgcaaatc catcgtcagc agccggtttg
    14521 taccggcggc taccagcagg ttggccacca gcttggcggt aatcggaaca cgcggctgat
    14581 ctttgcggtc ctgacgggcg tagccgtaaa acggcatcac agcggtaatc cgcgaagccg
    14641 atgcacgctt ggccgcatca atcatgatca gcagctccat cagattttcg ttcggcggat
    14701 aagaggtcgg ctgaataatg aatacatcac gcccgcggac attttcggcg atctttacaa
    14761 agatttcacc atccggaaac cggctgatct gcacatcgca gagcttttcg ccggctttag
    14821 cggcaatacg ttccgccaaa tcccgatggg ctgttcctga caaaattttc acagtttctt
    14881 cattccctat tataatgtgt tctcttgcgc cggttcatcc gccgagtggt ggatttgccc
    14941 gactaggatt tgaacctaga cgccaagaat caaaatcttg tgtgctacca ttacactatc
    15001 gggcagcccc gtttaatgac aaaaaaacac ccctccctcc acgagagaag cgttgaacca
    15061 taaaaagagc ccctgtcgtt gtcaaattaa aacgggaaat gaagagcatg cgatgcttac
    15121 gaaccgtttc agaccgttaa gtccgggctc tccgattttt tttcgtgcat ttgcacaggc
    15181 tttgaatatg ctccgcgcgt gaataaatac agtctcgccc cagttgtact gctatgcgca
    15241 gcgtttaacg gtattgcgca ggatgcaggt tcccgttttg atttcaacgc actcaaaaac
    15301 cgtgcgcaga atctggccgc ggcgccgtat caggccccgg accggtcgct gcccgaagag
    15361 ttgagccgtt ttaattacga cgacatgcgc aacatccgct ttgaccccaa agcggccatc
    15421 tggcgcatgg aaagactccc ctttcagatg cagttcacgc atcgcggcgg actgcagaac
    15481 agccacatca cgatgaatta catcgaagac gggcaggttg agccgattcc gttttccaag
    15541 aacctcttca actattcgca ggttaaaata aacggaaaac ttcccgaaga tctgggcttt
    15601 tacggcttcc gcatccatta cccgctcaac tccatggaat acctcgacga agtgatctcg
    15661 ttccagggcg cttcatactt ccgcgctctt ggcaaaggac tgcactacgg tctctcggcg
    15721 cgcggactgg ccctcaatgt ggcgggccaa accccggagg aatttccgga tttcatcgag
    15781 tactggattg aaaaaccgga tacccgggcc acccgcatca aaatcttcgc tctgctgaac
    15841 agcccgagcc ttgccggagc ttacgagatg acgctcatcc ccggttccga caccgtcatg
    15901 gaaatcaaaa ctgcgctctt cgcccgcaaa ccggtcgaag gtctggggct cgcgccgctg
    15961 accagtatgt tctggttcgg cgaacattcc accagccgcc acggcgattt ccgccccgaa
    16021 gtgcatgact ccgacggact gctcgtgcat accgccgccg atgaatggct gtggcgtccg
    16081 ctctccaatg aaggcctcgt gaagacctcc tgcttcgcca acgataatcc gcgcggcttc
    16141 ggcctcttcc agcgcgatcg ggatttctcc cactacgaag acctcgaaac cttctaccat
    16201 aaccgcccga gcgcctgggt tgagcccggt ccgggctggg gtcaaggcga actgcgcctg
    16261 gtggaacttc ccagcggcac ggaatacgcc gacaatatcg tcgccttctg gatgccgcag
    16321 gaacagctca cgccggaaca tccgctggaa tattcatacc gcctgcactg gttccgggac
    16381 gacgccaccc tgccgccgct ggcgcaggcg ctggcgaccc ggatcggcaa cgccacggaa
    16441 tatccgggct cgaaaaaatt tgtgctcgat ttcagcaatg cccccggtct tgaccgcaat
    16501 aatccggaca gcgtcacgcc ggtcatcacc ctctcgaacg gcaccctcaa agggaaacac
    16561 ctccagcgca acgacctcgg caacgcctgg cgcgtctttt ttgatgtaga accgatcgac
    16621 cggaccaagc cggtcgacat gcgctgcttc ctgcaatcag gcggccttcc ccggagtgaa
    16681 acgtggacct acttctggaa cccatagaaa ccttcgttcc aacctggggc agcccggagg
    16741 aatggaacag cgcgtatgaa aaactcgaga gctatctgcg ggctcatgag gttgacagtc
    16801 atttgcatcg cgcacatatc accaccatga ttttgcgccg ggtcagccgc cgctggcagg
    16861 gccagactcc gccggagccg ctcgccacgc tggccatcga agaagccaac cggctgctca
    16921 acgaatggtt ttctctcatt atggacctgc cggccggcgc gaatgcggac tttaccgccg
    16981 ccgacgggcg cgtcgccctg tttctctgcg acgggtcgtt gcgctggccc tacgcctttc
    17041 tgacgccgca tgtcccgaac gagctgaaaa ccgccatgcg cagcagcctg atgcgcgccg
    17101 ggccggaact gcagatcagc aacatggttc cgcgtccgat tgacctggga ctcattccgg
    17161 aatttgccga cgacgccatg gaccgactga accgcagccc gatcattaaa gcgctcatcg
    17221 gctggctgat ttttctagcg attctaacct acctcttctg gagaacacga cagccatgat
    17281 ccctgtaaaa ctcctgtcaa gcgcacagaa agtacgcagg cggattctct tcttttcact
    17341 ggtgctgctg ctgaccacgc tcgcttcctg ggtgatggcg gacatcctct ggcgcggcgg
    17401 catgaacagc tgcgaaatcg ccatcctgat tctcttcacc gttctttttc taccggtttc
    17461 cctcggcgtc gttcaggcgc tgacgggatt cttcatcctc tggcgccgcc gcgatgcgtc
    17521 ccgccagctt tctctgcttg agaaaatgga tccgccggtc gaactgcccg tcaccgccgt
    17581 cgtgttgccg attttcaacg aagatgtcag ccgcgtttac gaaggactgc gggccattta
    17641 ccgctccgtc gaaaaaaccg gcaaccttgc gaacttcgac ttcttcatcc tcagcgactc
    17701 caacgatccg aacaaatgga tcgaagagga aatggcgtgg gcggaactct gcaaacaggt
    17761 tcacggattc ggccgcattt tctaccgcaa acgcaaaatc gccctgaacc gcaaaagcgg
    17821 aaacatcagc gacttctgcc gacgctgggg cagcaaatac cgctacatgg tcattctcga
    17881 cgccgacagc gtcatgagcg gcgactgcct cgtcaaactg gcgcaactga tggaattgca
    17941 gccgcaggcc ggaattattc aggcggcgcc ggttcagtcc atgagcgaaa gctttttcgg
    18001 ccgcgtcatg caattcgccg gaacgctcta cggcccgatt ttccaggcgg gactcaactt
    18061 ctggcaggct gacgaaggca attactgggg ccacaacgcc atcatccggc ttgcgccgtt
    18121 catcgaacac tgcgcgctgc ccgacctgcc cgccagcaaa accccgcgcg ccaaattcat
    18181 gagccacgac tacgtcgaag ccgcgctgat gcgccgcgcc gggtatgaag tctggctggc
    18241 ctacgggctc ggcggaagct acgaaggcgg cccgccgacg ctgacggata tggccaagcg
    18301 cgaccggcgc tggtgccgcg gcaacttcca gcacagctgg ctgatgctct cgccgcacgt
    18361 ccgtccgatc aaccgcctgc acctcgccct cggcatcatg agctacctct cctcgccgct
    18421 ctggtttttc tttatgctgc tgggaaccat gcagttcgtc ctggcctcgc gcttcacgca
    18481 gcgcaccttc gacgccgatg tcggtctctc caaagtcctc gacatcggcg gcaaccggct
    18541 ggcgatcatt ctctttgcca ccaccatttc gctgctcttt ctgcccaaac tgctggggct
    18601 gctgctgatt ctgcggcata aacaggaggc caaacctttc ggcggccggt tgcgggcaac
    18661 cgtcagcgta gtgctggaac atctgacctc catgctcatt acgccggtac tcatgctctt
    18721 ccactcgcgc ttcgttctcg gcgtaatggc cggacaggaa gtcggctggg gttcgcagcg
    18781 ccgcggcggc gatgacggca ttgaatgggg cgaagccatc gaagcccact ggggccacac
    18841 cattatcggc gccgtttggg gtattctggc ctggtggatc aatccggcgt ttttctggtg
    18901 gatgagcccg atcttcatcg gactggtctg ttcgattccg ttttccgtcc tgctcagcaa
    18961 aacgtcggtc ggcgccgcct tgcgccgcac cggactgttt atgaccccgc aggagagctg
    19021 cccgccggtc gaactggccg aactgcaaac caatctcgac gcctgccagc agcacattcc
    19081 gccgcttgag cccctgcgga aaaactacgg cttcatgcag gtcgtcatcg acccgtacgt
    19141 caacgccatc cacatctcgc tcctgcggcg gcgcaacaaa caccgccggc tggacagcgc
    19201 ctatttcaca cagctgcaga accgggtcgt tacggaaggt ccggacgtgc tcagccgccg
    19261 cgaacagatg gcgctgctgc tcaaccccga atgcatggcc tggctccacg aacagatctg
    19321 gctgat
//
LOCUS       DAOPZO010000020        19046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_3014_length_19046_cov_8.414670, whole genome shotgun sequence.
ACCESSION   DAOPZO010000020 DAOPZO010000000
VERSION     DAOPZO010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 19046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19046
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3014_length_19046_cov_8.414670"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PKI68_02590"
     CDS             <1..344
                     /locus_tag="PKI68_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX52612.1"
                     /translation="TGKIINLLDRRVTSHDTTCIPYMLSKKGLEELTKLAALDLAPGI
                     TVNAVAPGAILPPPKDSNNPAWEYAGTIPLGKRPTPADIADAVLYLLKSDHITGQTIF
                     VDGGQNLLGME"
     gene            414..770
                     /gene="folB"
                     /locus_tag="PKI68_02595"
     CDS             414..770
                     /gene="folB"
                     /locus_tag="PKI68_02595"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNX52613.1"
                     /translation="MDKIYIRDLALRCIIGLYPEERKNKQDVIINIVMATDLRAAGMS
                     DSLNDTVDYKTIKLAVLDFVENSSFNLIESLAEGIAAICLKDGRVQSATVTIDKPGAL
                     RFCRSVAVEIVRSRTA"
     gene            complement(1003..1959)
                     /locus_tag="PKI68_02600"
     CDS             complement(1003..1959)
                     /locus_tag="PKI68_02600"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004344.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNX52614.1"
                     /translation="MPPFTLSFEKPVLELEKKLHELEHFSKKQDIDVSHEIERMKEKI
                     AATRGQIYGNLTAWQKVQVARHPERPYTMDYIRNMTTDFVEIHGDRIHADDRAIIGGF
                     AKIDGQKVMIIGSQKGRDTRSNLECNFGCAYPEGYRKALRLMRLADKFGVPVVTLIDT
                     PGAFPGLESEERHIAEAIAVNLREMFNIRVPIIVTIIGEGGSGGALGIGIGDRILILE
                     HAYYSVISPEGCAAILFKDRAYADQSAEALKITAQHLIENKLADEIVPEPKGGAHTDQ
                     KAAADNLKEALLRNLAELKTQTSEQLMNARYDKFRAMGVFQE"
     gene            complement(1978..2970)
                     /locus_tag="PKI68_02605"
     CDS             complement(1978..2970)
                     /locus_tag="PKI68_02605"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNX52615.1"
                     /translation="MADIFVKPFDEQHKPGGRFVKTGLIVLSVTVVVIGLCLWMDSRS
                     AKTRDEKPAAQEKPAAQPVKAPVPAPVAQSSTPSPAPVAPAASEADYKAQQLFTRAQS
                     LAAAGNLVQARDLLSEVVATATEENVKNNALRVQGRINIQLFLSPTPTPEKKTYIVQP
                     GDSLDKIARLNKTTVELVRKMNKIDNNLIYPGGRLLVPAAPFVVQVDKSDKTVDLMMN
                     GKLFKRYIAGLGVNGKTPVGTFYTVVHQTNPDWTPPGGGIIPFGDPRNLLGTRWMSIQ
                     DTTRPEIKGFGIHGTPARDSIGAETSNGCIRLLNEDVEELYLLIPRGTEVVISE"
     gene            3061..3819
                     /locus_tag="PKI68_02610"
     CDS             3061..3819
                     /locus_tag="PKI68_02610"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNX52616.1"
                     /translation="MRTATQDISGNPTEMTFGVQMLLIINIAAYVIEYIFHFPLSAFG
                     ALRANWWEHLAIWQLITYQFIHRGFWHIFWNMLGLYFLGPETERTLGTNRFFALYFLS
                     GVLGGLGWSLLSPDYAVCVGASAAVFGVLGAYAALYPNRELILIFLPFVPIKAWLFVL
                     LLGAYEFMHTLAGPGGGIANSAHLGGAIAGYVYATVAERTDLLWKVKEKFKAKAKPPV
                     SRAEIDRILDKAAQQGMHSLTSRERDLLKRAGKQ"
     gene            complement(3907..6465)
                     /locus_tag="PKI68_02615"
     CDS             complement(3907..6465)
                     /locus_tag="PKI68_02615"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX52617.1"
                     /translation="MSALKVKENLWWVGVQDHDIRVFDIVMFTEYGTSYNSFILKTGT
                     HTVLFETVKLKFFDRFLQNIKEVCDPSEIDYIVIDHTEPDHAGSLEKLLDYAPNAKVL
                     GSNVALSYLKDICNREIPGKAVADDEVIALDGIHLQFLSVPFLHWPDSIYTYIPELKT
                     LVTCDSFGCHYADDKVFNDRIEGDFTPAYRYYFNCIMGPFKPYVQYALGRIKPLAIET
                     ICCGHGPVLRKDVQHYIDLYDQWSRPEKKAKRSKPKVVTAFVSAYGYSERLAREISAG
                     VRDVIDADISLRDMVTEDHDKVLAEIKGADGFLLGTCTINGDALPPVMNLAMALNGIT
                     EGGKVAGVFGSYGWSGEGPDMVMSRLSLLRMKTVEPAFKTVFNPENKDKLAKARKYGQ
                     KFGKKLQEEWVKMKTDSAAGKTFWKCTVCGEVFEGPVPPQSCPVCGAGQEAFVEYAPE
                     VVTFRDDREMRIAVIGSGAGGIAAAEAVRKRNDKAQIDLYTREELLPYYRPVLTRALA
                     DKVEDTEYFMHPQNFYEKTRISLHLGATVESLSPRDKSFRLADGKTVKYDKLVLATGA
                     SCFIPPIQGADLPEVVALREKTDFDRLTGLLAGGPKKVAIVGGGLLGLETADALHRLG
                     HNVTVLEACPSVLPRQLDPGGSPLFTKIIQKSGIEIHYGVYVEEILGEGKVTGLKTRA
                     PQEIPADIVIVSAGIRPNVELAKAAGLKTDRGIEVNERMQTSDSDIYAVGDCAAVHGR
                     CGGLWEPASLQGNVAGANIAGDDKTYACKLYGATLAAFGTSLFSIGDLGAGDGKYEQI
                     MEYNELGGKYRKIYFKDDKVVGGVLLGDVKLTNPLLLSVTRGLDTETALEMMKL"
     gene            complement(6483..7172)
                     /gene="rnc"
                     /locus_tag="PKI68_02620"
     CDS             complement(6483..7172)
                     /gene="rnc"
                     /locus_tag="PKI68_02620"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNX52618.1"
                     /translation="MKQRAFKTLEKRIRYRFKKPALLEAALTHPSFRYENPDAAADNQ
                     RLEFLGDAVLGLLAADILMTRNPQAAEGELTKLKSAVTSGSALAAKARLIGLGEHLRL
                     GKGEAASGGADRGSNLEDALEALLGAIWLDGGLKAARKFFENTVFQSAENSEPVIDNP
                     KGMLQEYAQKKGYPVPDYQVLEASGPDHARRYCVEVTVSTYAFRGEGTSRREAEKMAA
                     EKAVKCMIQKD"
     gene            7234..7644
                     /locus_tag="PKI68_02625"
     CDS             7234..7644
                     /locus_tag="PKI68_02625"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNX52619.1"
                     /translation="MDFVFGICHKQTMPRPQKERRIGHCAPVTYFKPQGIPLRMLEAV
                     ELAPDELEAIRLADYEELYQEQAAEKMGVSRQTFGLIISRAHKKVAEALTHGKAIRVE
                     GGKEITLSEIPDAPQGGCRCGRGGHGHGHGCRQI"
     gene            7662..7919
                     /locus_tag="PKI68_02630"
     CDS             7662..7919
                     /locus_tag="PKI68_02630"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HNX52620.1"
                     /translation="MPRGDRTGPAGAGPMSGRGAGFCAGTNTAGFQTAGGGFGRGCGR
                     GFGFRNRFAQPVQTLQGDELLELKEQMARIEQRLGEIQKKS"
     gene            7916..8260
                     /locus_tag="PKI68_02635"
     CDS             7916..8260
                     /locus_tag="PKI68_02635"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HNX52621.1"
                     /translation="MKQTAIEHYKNRRGNCAQAVALAWKDKKQPESDHHIRFADFGSG
                     QAPEGLCGALYAACELGGEQHKEDLKEKFSQQASGHITCRGIRQNRVMPCSECVATAA
                     GLLEELVKENSK"
     gene            8257..8619
                     /locus_tag="PKI68_02640"
     CDS             8257..8619
                     /locus_tag="PKI68_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNX52622.1"
                     /translation="MKIAVTTSGNDLSAPLDPRFGRAARFIIYETETGSFEIADNEQN
                     LNAAQGAGIQAAQNAASTGAAAVISGHFGPKAFRVLSASKIAVYPTTAATVREALDLY
                     ASEKLTAAQKADVEGHWM"
     gene            8705..9565
                     /locus_tag="PKI68_02645"
     CDS             8705..9565
                     /locus_tag="PKI68_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HNX52623.1"
                     /translation="MKTCLDCMPCFVRQTLDAARMSSNDPAVHEQIILDVLRWLSEMD
                     LSISPPALAQRIHRRLRELTGVEDPYRKEKDQHNQMALQLLPGLRARVNVSDDPLMTA
                     ANLAVAGNIIDLGAKSGLTEHEIHDALRHAAEQPLAGDREHFRQEVERAAKILYLADN
                     CGEIVFDTLLVEQLGTARVTVAVRGRPVINDATLEDARTAGLTSLVHVIGNGSDAPGT
                     LLNDCNDEFKTAFESADLIVSKGQGNFETLSHCEKNIFFLFKAKCPVVANSINLPIGT
                     HVLRCGRRRL"
     gene            9565..10389
                     /locus_tag="PKI68_02650"
     CDS             9565..10389
                     /locus_tag="PKI68_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX52624.1"
                     /translation="MQIAIASGKGGTGKTTLSVALAQAWGGPVQLLDCDVEEPNASIF
                     LNLEDAAEQTVSVPVPAVDSSLCTGCGKCAAICQFNALAVAGKSVLVFAELCHSCGGC
                     VRICPEKAITEVPLSVGIIRRGQSGNIQFMEGRLNIGRAMAPPVIRAVKKAADPALPA
                     LIDCPPGTSCPMITAVKGSDFVILVTEPTPFGLHDLKLAVETVRLLNLPFGVVINRSD
                     AGDNRVVEYCEKENIRLLLQIPESRKIAEAYSRGESILTAAPELKEALRNLLEELR"
     gene            10386..11255
                     /locus_tag="PKI68_02655"
     CDS             10386..11255
                     /locus_tag="PKI68_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX52625.1"
                     /translation="MKELVIISGKGGTGKTSITASFATLAKNAVLADCDVDAADLHLI
                     LNPEIRKRHDFYSGHEAVIRGTDCTGCGLCAELCRFDAVKKFEEKFRIEPSSCEGCGV
                     CVEFCPVKAIDFPDRLCGDWFVSKTRCGTMVHARLGIGAENSGKLVSTVRQQAKHFAT
                     AVQAEWILVDGPPGTGCPVIASITGADALLAVTEPTLCGQHDLLRILELARHFQIPAF
                     VCVNKWDINPALTAAIEAASTGAGATVLTRIPYDRSITEAQINGQSVVEHNDGTASQF
                     IQTLWEKLCQLLH"
     gene            11237..12457
                     /locus_tag="PKI68_02660"
     CDS             11237..12457
                     /locus_tag="PKI68_02660"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNX52626.1"
                     /translation="MSAAALDGNITLPGVFGPLKGANGNARITGPCGDTMEFWVKIQD
                     ERILRCTFTTDGCSDSVACGTAACTLASGAPESVAFSLEPADVLALIPGLAEESKHCA
                     LLAITTLRAAISDYRKNHCPPEACSSCEKKCSSRKETTSPVQTAPNGLSRVKHKIVVL
                     SGKGGVGKSTVAAHLALSLSAKGFNVGLLDADLHGPSIPTLFNLSGTHAEMFGDTIEP
                     VRAGGVKLMSTGFMLEHPDQAVIARGPMKIKIIEQMLKEVNWGDLDYLITDCPPGTGD
                     EPLSVCQLIGSPDGAVIVTTPQEIAAADVRKSVQFCRKIDLPILGILENMSGYTCPKC
                     GETVHLFGKDGGFKTAEKYNIAFLGRIPIDPNVGICGDAGQSFLQKHSGSAAAQAFET
                     AVEQIGVAVRGSFS"
     gene            12454..13290
                     /locus_tag="PKI68_02665"
     CDS             12454..13290
                     /locus_tag="PKI68_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX52627.1"
                     /translation="MTQIKITVLVENAAGKRDLLAEHGLSFLIGSGRNKVLFDTGQGL
                     ALRHNTATLNVDLKAINAVVLSHGHYDHTGGLTFALNRMNCPRLYVHPEAFSLKYARN
                     PDGSAREVGMTAGNKITAGELAEIVLTDERTEIVDGLFLTGPVPRITDFEDTGGAFFK
                     DQECRNPDELPDDQAAFMETKNGTVVILGCAHSGVINTLRYIQTLTHNRPIHTVIGGM
                     HLLHASPERMDQTVAELRRLNIQRLIPCHCTGFAAMVRLWKEFPGKCETCPSGTVIQI
                     ED"
     gene            13298..13543
                     /locus_tag="PKI68_02670"
     CDS             13298..13543
                     /locus_tag="PKI68_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNX52628.1"
                     /translation="MIKLYTSKHCPICEQVKAALKPISHQILSAEDHMDDLIKEVTDR
                     KLDHRQIRQAPVMVVPGCDLIWTGADCLIAIEEKEWE"
     gene            13700..14038
                     /locus_tag="PKI68_02675"
     CDS             13700..14038
                     /locus_tag="PKI68_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNX52629.1"
                     /translation="MKIAIPVTNEKLCMHFGHCETFAVLTVDESAKRITGRQDMVPPP
                     HEPGVLPRWLGEQKVNIIIAGGMGQRAQQLFAEQNIAVVVGAPAETPETLVTDYLAGT
                     LESGANVCDH"
     gene            14189..14950
                     /locus_tag="PKI68_02680"
     CDS             14189..14950
                     /locus_tag="PKI68_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNX52630.1"
                     /translation="MKRLSFALPLLVLAAVMAGCTHPMTVTNMDNYHNRTIVSLDERL
                     RIGIRANAADLNGHRMIYAIGRDLLKYNAQSTTAIEETNEYVDVIANISVVSKQRGSG
                     WNFWADFPGFLIWFPSWHGYNYDVMYDIDVTLNDARTGKLINSMYIPVRFDVKHADIS
                     RTWVDGVAWPTLGVSALIGGLHNMTYDETVTPLVEQDATPILADYVAQQIAFTLHYYK
                     SAPRDRFEKLKMLLDEKLISQEEYDMKRKAVINDI"
     gene            complement(15000..15905)
                     /gene="xerD"
                     /locus_tag="PKI68_02685"
     CDS             complement(15000..15905)
                     /gene="xerD"
                     /locus_tag="PKI68_02685"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNX52631.1"
                     /translation="MVNILFMRALLEQFLDYISLEMGLSPNTRAAYADDIGRFLDWLS
                     QKGVRSINEVTRKQILDHLMAEKERGLAANSLSRHLVSLKVFFRYLVQEGLLSINVAE
                     TMESPKVWKMLPSVLTPKEIDRLFAAPDLTTPLGIRNLAILQIFYASGLRVSELAGLQ
                     LHDLHFDEHFLRCVGKGRKERVVPVGKEAIEKVKQYIKEVRPQLTPGPQEQTVFLSSR
                     RQPMSRKTIWDMIKKCARAAGITKTIYPHTLRHSFASHLLANGAPLRIIQEMLGHADI
                     ATTQIYTHVDPDRLKGVHRQFHPRA"
     gene            complement(15965..16855)
                     /locus_tag="PKI68_02690"
     CDS             complement(15965..16855)
                     /locus_tag="PKI68_02690"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNX52632.1"
                     /translation="MSQALTKIQIPGVPAPRSGKVREIYDAGDKLLFVASDRISAFDC
                     VMPNGIPKKGSVLNMISKFWFERTGDIVKNHMITTDVSKFPAPFNQHADQLAGRSMLV
                     KKTTVFPVECIVRGYLIGSGWKEYKQSGTIGGMPLRAGYKMADKLDEPIFTPSTKAEM
                     GLHDENISFEKAKEIVGAAKAEKLKAISIALYKRAAEYALTKGIIIADTKFEFGELDG
                     EIILIDEALTPDSSRFWPADTYKVGSSPMSYDKQFVRDYLETLDWDKTPPAPALPEEI
                     IVKTQAKYLEAYERLTGAKL"
     gene            complement(16870..18027)
                     /gene="nifS"
                     /locus_tag="PKI68_02695"
     CDS             complement(16870..18027)
                     /gene="nifS"
                     /locus_tag="PKI68_02695"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR03402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HNX52633.1"
                     /translation="MRTVYLDNNATTKIADEVRESMMPFLTDLWGNPSSMHTFGGQIK
                     KYVEAAREQVAALIGAADPQEITFTSCGSESDNLAIRGGAEAMDKPPHVITSKVEHAA
                     VSGPCHYLEDRGFRLTKIDVDQLGRLDLDALRREVKSSKTLTSIMWANNETGVIFPVG
                     LIGEMVHEAGGIFHTDAVQAVGKIPMKVSDLPVDMLSLSGHKLHAPKGVGAIYIRRGT
                     KVKPLVLGGHQERGRRAGTENVPYIVGLGVACDLAGKHLNEENTRVKALRDRLEQGIL
                     ATCPGARVNGDPKCRLPNTTNISFDFIEGEAILLMLDDRGICASTGSACASGSLEPSH
                     VLRAMNVAGVSVHGSIRFSFSTYNTDEDVDAVLTELPKVVKRLRELSPFVN"
     gene            complement(18027..18866)
                     /gene="nifU"
                     /locus_tag="PKI68_02700"
     CDS             complement(18027..18866)
                     /gene="nifU"
                     /locus_tag="PKI68_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HNX52634.1"
                     /translation="MWDYTDKVNDHFKNPRNVGKIEKPDGTGEVGSLACGDALKLMFK
                     LDETGRIKEAKFQTFGCASAIASSSALTEMIIGKTLEEAEQITNKDIAEYLGGLPKQK
                     VHCSVMGREALEAAIYNYRTGKKFDKDLEGEVICHCFGVTDKEIERVTRENNVQTLEE
                     LTNACKAGGGCGNCLPELQKIIDRVHGEMAATSAVPEKKPLTNLQKIHLIEDVIDREI
                     RPALRQDGGDLELIDVDGDTVKVRFRGACSGCVVSAITLKDVVEARLKENVSEDIRVE
                     EVK"
BASE COUNT         4156 a         5458 c         5261 g         4171 t
ORIGIN      
        1 agaccggcaa gatcatcaac ctgctcgacc gtcgcgtcac ttcgcacgac acgacctgca
       61 ttccgtacat gctcagcaaa aaagggctcg aagagctgac gaaactggcg gcgctcgacc
      121 tcgcgcccgg catcaccgtc aacgccgtcg cccccggtgc catccttccg ccgccgaagg
      181 acagtaataa tccggcgtgg gaatatgccg gaaccatacc gctcggcaaa cgcccgacac
      241 ccgccgatat cgccgacgcg gtgctctatc tattgaagag cgaccacatc accggacaga
      301 cgattttcgt agacggggga cagaatctgt tgggaatgga atagcggttt caaggccggc
      361 tggaagccgg cggtacgtat cgccggcttc cagccggcct tgggagtttt attatggata
      421 agatttatat ccgagacctg gcgctgcgtt gcatcatcgg cctgtatccc gaagagcgga
      481 aaaacaagca ggacgtcatc attaacatcg tgatggcaac cgacctgcgg gccgccggaa
      541 tgagcgattc gctgaacgac acggtggatt acaaaaccat caagctggcg gttctggatt
      601 tcgtcgaaaa cagcagcttc aatctgattg agtcgctggc ggaaggcatc gccgcgatct
      661 gcctgaaaga cggccgcgtt cagagcgcca cggtcacgat tgataaaccc ggcgccctgc
      721 gtttctgccg cagcgttgcg gttgaaatcg tgagaagccg tactgcgtaa cgagtactgc
      781 gtattgcagg aaagtcctga agcaattcat gattgaaaaa ccgtacttac ccaccccggc
      841 tgtcacttcg ttctatccac ccctccttgg gaggggaact ttataatccc ctccgacgga
      901 ggggtgcccg cagggcgggg tgggtttgga atcattatct gctctaagct tcctggagtc
      961 tggaaatccg gatgttacgc agtacgcaat acgtgatacg aattactctt ggaacacgcc
     1021 catggcgcgg aatttgtcgt agcgcgcgtt catgagctgt tcggaagttt gcgttttgag
     1081 ttccgccaga ttgcgcagca gtgcttcttt gagattatca gcggcggcct tctgatcggt
     1141 atgcgccccg cccttcggtt cgggcacaat ttcgtcggcc agcttgtttt cgatgaggtg
     1201 ctgagcggtg attttcaacg cttccgccga ctggtcggca taggcacggt ctttgaagag
     1261 aatcgcggcg cagccttccg gtgaaatgac ggaatagtag gcgtgttcga gaatcaggat
     1321 gcggtcgccg atgccgatgc cgagcgctcc gccggagccg ccttcgccga tgatggtgac
     1381 gatgatcggt acgcggatgt tgaacatttc gcgcaggttg acggcaatcg cttcggcgat
     1441 gtggcgctct tcggattcaa gaccgggaaa tgcgccgggg gtgtcgatga gggtgacaac
     1501 cggcacgccg aatttgtcgg ccagacgcat gaggcgcagg gctttgcggt agccttcggg
     1561 ataggcgcag ccgaagttgc attccagatt gctgcgggtg tcgcgccctt tctgcgagcc
     1621 gatgatcatg actttctgcc cgtcaatctt tgcgaaaccg ccgatgatgg cgcggtcgtc
     1681 ggcatggatg cggtcgccgt ggatttcgac gaagtcggtg gtcatgttcc ggatgtagtc
     1741 catggtgtac ggacgctccg gatggcgggc gacctgtacc ttctgccacg cggtcaggtt
     1801 gccgtagatc tgcccgcggg tcgccgcaat cttttccttc atgcgttcga tctcgtgaga
     1861 gacatcgatg tcctgtttct tgctgaagtg ttccagctcg tgaagtttct tctcgagctc
     1921 aagcacaggt ttttcaaaag acagtgtaaa aggcggcata gcacaactcc gggttggtta
     1981 ttcgctaatg acaacttccg ttccgcgggg aatcagaagg tagagttctt cgacatcttc
     2041 gttcagcaga cggatacagc cgttgctggt ttcggcgccg atactgtcgc gcgccggcgt
     2101 tccgtgaatt ccaaagcctt tgatttccgg acgggtcgta tcctgaatcg acatccagcg
     2161 ggtgccgagc aggttgcgcg gatcgccgaa cgggatgatg ccgccgccgg gcggggtcca
     2221 gtccggattg gtctgatgca cgaccgtata gaacgttccg accggcgttt ttccgttcac
     2281 gccgagtccg gcgatatacc gtttgaacag cttgccgttc atcatcaaat caacggtttt
     2341 atcggatttg tccacctgaa cgacaaacgg agcggcagga accagcaggc ggccgccggg
     2401 ataaatcagg ttgttgtcga ttttgttcat tttccgaacc agctcaaccg tggtcttgtt
     2461 cagacgcgcg atcttatcaa gcgagtcgcc gggctggacg atataggtct ttttctcagg
     2521 cgtcggcgtc ggcgaaagaa acagctggat attgatacgg ccctgaacgc gcagtgcgtt
     2581 gttcttcaca ttttcctcgg tggcagtcgc aaccacttca ctgagcagat cgcgcgcctg
     2641 gacgagatta ccggcggcgg ccagactctg agcgcgggta aaaagctgct gagccttata
     2701 atcggcttcc gaagccgccg gagcgaccgg cgcaggtgac ggtgtcgagg actgcgcaac
     2761 aggcgccgga accggagctt taaccggctg ggcggccggt ttttcctgcg cggcaggttt
     2821 ttcatcccgc gttttggctg aacggctgtc catccacagg cacagcccga tcacaaccac
     2881 cgtaacggaa agtacaatca gtccggtctt aacaaaccgc ccgccgggtt tatgctgctc
     2941 atcaaaaggt tttacgaaaa tatctgccat ttttatgtcc tgaatctctg caattggggg
     3001 gagaaactaa ttcaaacccc gtgcttttgc aaaccattaa aaatcggcta ttgtctcccc
     3061 atgcgaactg caactcagga tatttccggc aacccgacag aaatgacttt cggcgtccag
     3121 atgcttctca tcatcaacat cgccgcctac gtcattgaat acatctttca ctttccgctg
     3181 agcgctttcg gcgcgctgcg cgccaactgg tgggaacatc tcgccatctg gcagctcatc
     3241 acctaccagt tcatccatcg ggggttctgg cacatcttct ggaacatgct tggactttat
     3301 ttcctcggcc cggaaaccga acggacactc ggcaccaacc gcttcttcgc cctctacttc
     3361 ctgagcggcg tactcggcgg actcggctgg tcgctgcttt ccccggacta cgccgtttgc
     3421 gtcggcgcct ccgccgccgt cttcggcgta ctcggcgcct acgccgcgct ctacccgaac
     3481 cgcgaactca tcctcatctt cctgcccttc gtaccgatta aagcgtggct cttcgttctc
     3541 ctgctcggcg cgtatgaatt catgcacacc ctcgccggac ccggcggcgg catcgccaac
     3601 tccgcgcatc tcggcggtgc catcgccggt tacgtctatg caaccgtcgc cgaacgcacc
     3661 gacctgctgt ggaaggttaa agaaaagttt aaagccaaag cgaaaccgcc ggtcagccgc
     3721 gccgagatcg accgaatcct cgacaaagcc gcgcagcaag gtatgcattc gctgacgagc
     3781 cgtgaacgcg acctgctcaa acgcgcaggg aaacaataac tcgtaacgag tactgcgtaa
     3841 ttcgcaacgg aagattttta ttccatcgcg ccgaagtaaa ttacgtatta cgcagtactc
     3901 attacgctac agcttcatca tctccagcgc ggtttcggtg tcgaggccgc gggtgacgct
     3961 gagcagtaac ggattggtca gtttcacgtc gccgagcagt acgccgccga ccaccttgtc
     4021 gtccttgaag taaatcttgc ggtattttcc gcccagctcg ttgtactcca taatctgttc
     4081 gtactttccg tcgcccgcac cgaggtcgcc gatggaaaag agcgaggttc cgaaggcggc
     4141 cagcgtcgca ccgtagagtt tgcacgcata ggttttgtca tcgcccgcga tattggctcc
     4201 ggcgacattg ccctgcagcg acgccggttc ccacagtccg ccgcagcgtc cgtggacggc
     4261 ggcgcagtcg ccgacggcgt agatatcgga atccgacgtc tgcatccgtt cgttgacttc
     4321 aataccgcga tctgttttga ggcccgccgc ttttgccagc tcgacgttcg ggcgaatccc
     4381 ggcggaaaca atgacgatgt cggccggtat ttcctgcggc gcgcgggttt tcagtccggt
     4441 cactttgcct tcgccgagaa tttcttcgac gtaaacgccg tagtgaattt cgatgccgct
     4501 tttctgaatg attttggtga agagcggtga gccgccgggg tcgagctggc gcggcagtac
     4561 cgacggacag gcttcgagca cggtcacatt atggccgagc cggtgaagcg cgtcagcggt
     4621 ttctaggccg agcagtccgc cgccgacaat cgcgaccttt ttcggtccgc ccgcgagcag
     4681 tccggtcagc cggtcaaagt cggtcttttc gcgcagcgcc accacttcgg gcagatccgc
     4741 gccctgaatc ggcggaataa agcaggacgc gccggtggcc agcaccagct tgtcgtactt
     4801 caccgtttta ccgtccgcca gccggaacga cttgtcgcgc ggcgacaggc tttcgaccgt
     4861 cgcaccgagg tgcaggctga tccgggtttt ttcatagaaa ttctgcggat gcataaagta
     4921 ttcggtgtct tcgactttgt cggccagcgc gcgcgtcagc accgggcggt aatacggcag
     4981 aagttcttcg cgggtgtaga gatcaatctg cgctttgtca ttccgttttc gcaccgcttc
     5041 ggcggcggca atgccgcccg cgccggaacc gatgacggca atacgcattt cacggtcgtc
     5101 gcggaaggtg acaacttccg gtgcgtactc gacaaacgcc tcctgccctg cgccgcagac
     5161 cgggcagctc tgaggcggaa ccgggccttc gaatacttcg ccgcaaaccg tgcacttcca
     5221 gaatgtttta ccggcggctg aatcggtttt cattttcacc cactcctctt gcagtttttt
     5281 gccgaacttc tgcccgtact tgcgcgcctt ggccagcttg tctttgtttt ccggattgaa
     5341 aaccgttttg aatgcgggct cgacggtttt catacgcagc agactcaggc ggctcattac
     5401 catgtccggc ccttcgccgc tccagccgta actgccgaaa actccggcga ctttaccgcc
     5461 ttcggtgatt ccgttaagcg ccatcgccag attcataacc ggcggcagtg cgtcgccgtt
     5521 gatcgtgcag gtgccgagca gaaagccgtc ggctcctttg atttcggcca gaaccttgtc
     5581 gtggtcttcc gtcaccatgt cgcgcaggct gatgtcggca tcgatcacgt cgcggactcc
     5641 ggcgctgatt tcgcgcgcca gccgttcgct gtagccgtag gcggaaacaa acgccgtcac
     5701 cactttgggt ttgctgcgtt ttgctttttt ctccggtctg ctccactggt cgtagagatc
     5761 aatataatgc tgaacatcct tgcgcagcac cggcccgtga ccgcagcaga tcgtttcgat
     5821 tgcgagcggt ttaatgcgtc ccagcgcgta ctgcacgtac ggcttgaatg ggcccatgat
     5881 acagttgaag taataacggt aggccggtgt gaagtcgcct tcgatccggt cattgaaaac
     5941 cttgtcgtcg gcgtagtgac agccgaacga gtcgcaggta accagcgttt tcagctccgg
     6001 aatgtaggtg tagatcgaat ccggccagtg cagaaacggt acggaaagaa actgaagatg
     6061 gatgccgtcc agcgcaatca cttcatcgtc ggcgaccgcc ttgccgggaa tctcgcggtt
     6121 gcagatatct ttcagatagc tcagcgccac attcgagccg agcaccttgg cgttcggcgc
     6181 gtagtcgaga agcttttcga gcgaaccggc gtggtcaggt tccgtgtggt cgatcacgat
     6241 gtagtcgatc tcggacgggt cacagacctc tttgatgttt tgcagaaacc ggtcgaagaa
     6301 cttcagcttc accgtttcaa acagcaccgt atgggttccg gttttcagaa tgaaactgtt
     6361 gtagctggtg ccgtactcgg taaacatcac gatgtcaaaa acccggatgt catgatcctg
     6421 caccccgacc caccagagat tttctttaac ttttagcgca ctcatttttc agctcctgca
     6481 aatcaatcct tttgaatcat acatttcacc gctttttcag cggccatttt ttcggcttcg
     6541 cgccggctgg ttccttcgcc gcggaaggcg taggttgaaa ccgtcacctc aacacagtaa
     6601 cgccgggcgt gatccggccc gcttgcctca agcacctgat aatcgggaac cggatagccc
     6661 ttcttctgcg catattcctg aagcatcccc ttcggattat caatcaccgg ctctgaattt
     6721 tccgctgact gaaaaacagt gttctcaaaa aacttccggg ccgctttgag tccgccgtcc
     6781 agccagatcg cgccgagcaa agcctccagc gcatcttcca ggtttgaccc gcggtcggcg
     6841 ccgccgctgg ccgcctcgcc tttgcccagt cgcaggtgtt cgcccagtcc gatcagccgg
     6901 gcctttgccg ccagcgctga cccggaagta acagcgctct tgagttttgt cagctcgcct
     6961 tccgccgcct gcgggttgcg cgtcatcaat atatcggcgg ccagcagacc gagtactgcg
     7021 tcgcctagaa attccaaccg ctgattgtcg gccgccgcat ccggattttc atagcggaac
     7081 gacggatgcg tcagagcggc ttccagcagc gccggtttct tgaagcggta cctgatgcgc
     7141 ttctccaggg tcttaaaggc tctctgtttc attcgctgtt tataccctct gttcctcagc
     7201 aaccggaaga tttttcagca aaccaaaaat taaatggact ttgtttttgg catatgccat
     7261 aaacagacca tgcctagacc tcaaaaagaa cgacggatcg gccattgcgc gccggtgact
     7321 tatttcaaac cgcaaggcat cccgctacga atgctggaag ccgtagaatt ggctccagat
     7381 gaactggaag ctatccgact ggccgattac gaagaactct atcaggagca ggccgccgag
     7441 aagatgggcg tttcgcgcca gaccttcgga ctgattatct cccgggcgca caaaaaagtg
     7501 gcggaggcgt tgacacacgg aaaagccatc cgtgtcgaag gcggaaaaga aataaccctg
     7561 tccgaaattc cggacgcccc gcagggcggc tgccggtgcg gacgcggcgg gcatggacac
     7621 ggtcacggct gccgtcaaat ttaaacaaaa ggaggcatac aatgccaaga ggagacagaa
     7681 caggtccggc gggcgcaggc ccgatgagcg gacgcggcgc agggttctgc gcaggaacca
     7741 acacagccgg tttccaaacc gccggcggag gcttcggacg cggatgcgga cggggcttcg
     7801 gattccgcaa ccggttcgcg caacccgttc agacgttaca gggcgatgaa ctgctcgaac
     7861 tgaaagaaca gatggcgagg attgaacagc ggctcggaga aatccagaaa aagtcatgaa
     7921 gcaaaccgct atcgaacact acaaaaaccg ccggggcaac tgcgcacagg ccgttgccct
     7981 ggcgtggaaa gataaaaagc agccggagtc ggatcatcat attcgttttg ctgacttcgg
     8041 cagcggacag gctccggaag gactttgcgg cgcactgtac gcagcctgcg agctgggcgg
     8101 tgaacagcac aaggaagacc ttaaagaaaa attcagtcaa caggcatccg ggcatattac
     8161 ctgtcgggga atacggcaaa accgggttat gccctgctcc gaatgcgtcg caaccgccgc
     8221 cgggctgctg gaagaactgg ttaaggaaaa ttcaaaatga aaattgcagt caccacatcc
     8281 ggaaacgacc tgagcgctcc gctcgatccg cgcttcggac gcgccgcacg gtttatcatc
     8341 tatgaaacag agaccggatc ttttgaaatc gcggacaacg agcagaacct caacgccgcg
     8401 caaggcgccg gaattcaggc cgcgcagaat gcggcctcga ccggcgcggc agcggttatc
     8461 agcggacact tcggcccgaa ggcgttccgg gttttaagcg cttcgaaaat agcggtttat
     8521 ccgaccaccg ccgcgacggt tcgggaagcg ctggatttgt atgcatcgga aaaactgact
     8581 gccgcgcaga aagcggatgt ggaaggacat tggatgtgag cgttttcgat ttcagattga
     8641 tgactgccga tttaatcaaa aaacaacccg gcggccactt ggcgccctcc acttgccact
     8701 cattatgaaa acctgtctcg actgtatgcc ttgttttgtc cggcagacgc tggatgccgc
     8761 ccggatgagc tcaaacgatc cggctgttca cgaacagata atcctcgatg tgctgcgctg
     8821 gttgagcgaa atggatttgt ccatttcgcc gcctgcgctg gcgcagcgca ttcaccgtcg
     8881 tctgcgcgag ctgactggcg ttgaagaccc gtaccggaag gaaaaagatc agcacaatca
     8941 aatggcattg cagctgctgc ccggattgcg cgcgagggtc aatgtctcgg atgatccgct
     9001 aatgactgcg gccaatttgg ccgtcgccgg aaatatcatt gatctgggag ccaagagcgg
     9061 actcaccgag catgaaattc acgatgcact ccggcacgcc gccgaacaac cgctcgcagg
     9121 cgaccgggaa catttccggc aggaagttga acgggcggcc aaaattcttt atctggccga
     9181 caactgcggc gaaatcgttt tcgacacgct gctggttgaa cagctcggca cagcgcgggt
     9241 gacagttgcc gtacgcggac gtccggttat taacgatgcc acgctggaag atgcgcgaac
     9301 agccgggctc accagtctcg tccacgtcat cggaaacggc tccgacgcgc caggaaccct
     9361 gcttaacgat tgcaatgacg aattcaaaac cgctttcgag tctgctgatt tgattgtctc
     9421 caaaggtcag ggcaattttg aaacgctcag tcactgtgaa aaaaacattt tcttcctgtt
     9481 taaagccaag tgcccggtcg tggcgaattc gatcaacctg ccgatcggca ctcatgtgct
     9541 tcgctgcgga aggcggaggc tgtaatgcag attgcgattg catccggaaa aggcggaacc
     9601 ggcaaaacaa ccctgtcggt cgcgctggct caagcgtggg gaggccccgt tcaactgctc
     9661 gactgcgatg tcgaagaacc gaacgcttcg atcttcctga atctggaaga cgccgcagaa
     9721 caaacggttt ccgttccggt tcctgccgtg gattcctctc tctgcaccgg atgcgggaaa
     9781 tgcgcggcga tctgtcagtt caacgcgctc gccgttgccg gaaaatccgt gctggtgttt
     9841 gcagaactgt gccacagctg cggcggctgc gtacgaatct gccctgaaaa ggcgattacg
     9901 gaagttccgc tttctgtcgg aataatccgg cgcggacaaa gcggaaatat acagttcatg
     9961 gaaggccgtc tgaatatcgg ccgcgccatg gctccgccag tcatccgcgc agtcaaaaaa
    10021 gcagcagatc cggctttgcc ggctttgatc gactgtccgc cgggcacctc ctgcccgatg
    10081 attaccgccg ttaaaggctc ggattttgtc atcctcgtca ctgaaccgac accgttcgga
    10141 ctgcatgatc tgaaactggc ggtcgaaacc gtgcgactgc taaacctgcc gttcggcgtc
    10201 gtcatcaacc gctccgatgc gggcgataac cgcgtggtcg agtattgcga gaaagaaaat
    10261 atccgcctgc tgctgcaaat tccggaatcg aggaaaatcg ccgaagccta ttcgcgaggc
    10321 gaaagcattc tgacggcggc accggaactc aaagaagcgc tgcgaaatct gttagaggaa
    10381 ctccggtgaa agaactggtc atcattagcg gcaaaggcgg aaccggcaaa accagcatca
    10441 cggcgtcctt cgccacgctc gcgaaaaatg cggtgctggc cgactgcgat gtggatgccg
    10501 ccgacctgca tctcattctg aatccggaga tccgcaagcg gcatgatttt tacagcggac
    10561 acgaagccgt tattcgcggg acagattgca cgggatgcgg cttatgcgcc gagctctgcc
    10621 ggtttgatgc cgtcaaaaag ttcgaagaaa aattccgaat cgaaccgtca tcctgcgaag
    10681 gctgcggcgt ttgcgtcgag ttctgcccgg tcaaggcgat tgattttccg gaccggctgt
    10741 gcggcgactg gttcgtttca aaaacccgct gcggcacgat ggttcatgca cgccttggta
    10801 tcggcgcgga aaattcggga aaactggtca gtactgtccg gcagcaagcc aaacactttg
    10861 caacagcagt tcaggctgag tggattctgg tagacggccc gcccggcacc ggatgtccgg
    10921 tcatcgcgtc catcaccggt gcggatgcgc tactggccgt tactgaaccg acgctttgcg
    10981 gacagcacga tttgctgcgg atcctcgaac tggcccgcca ctttcagatt ccggcgttcg
    11041 tctgcgtcaa caagtgggac atcaatccgg cattgaccgc cgccattgag gctgcatcca
    11101 caggtgccgg cgccaccgtt ttaacgcgga ttccgtacga ccgttccatt accgaagccc
    11161 aaattaacgg gcagagtgtg gtggaacata acgacggaac cgcttcacaa ttcatacaaa
    11221 ccttatggga gaaactatgt cagctgctgc actagatgga aatataaccc tgcccggcgt
    11281 gttcgggccg cttaaaggcg ccaacggcaa tgcacgaatc accggcccgt gcggcgatac
    11341 gatggagttc tgggtgaaaa ttcaggacga acggatactc cgctgcacct tcacaaccga
    11401 cggctgctcc gattcagtcg cctgcggaac cgcggcctgc acgctggcct ccggcgcgcc
    11461 ggaatccgtt gcattttcgc tggagcctgc cgatgtcctc gcgctgattc cgggtcttgc
    11521 cgaggaatcc aaacactgcg ccctgctggc gatcaccacc ttgcgggccg ccatttcgga
    11581 ttaccgtaaa aaccactgcc cgccggaggc ctgctcctct tgcgaaaaaa agtgcagttc
    11641 gcgcaaggag accacctctc cggttcaaac cgcaccgaac ggactctcca gagtgaagca
    11701 taaaatcgtg gtgctttccg gaaaaggcgg cgtcggaaaa agcacggtcg cggctcatct
    11761 ggcgctgtcg ctttccgcca aaggattcaa cgtcggactg cttgatgccg acctgcacgg
    11821 acccagcatc cccaccctgt tcaatctgtc cggtacacac gctgaaatgt tcggcgacac
    11881 catcgagccg gtacgagcag gcggagtcaa actgatgtca acaggcttta tgctggagca
    11941 tccggatcag gccgtcatcg cccgcggtcc gatgaaaata aaaatcattg aacagatgct
    12001 gaaggaagtg aactggggcg atctggatta tctgattaca gactgcccgc ccggaaccgg
    12061 cgacgaaccg ctttccgtct gccagctgat cggctcgccg gacggcgcag tcattgtcac
    12121 cacgccgcag gaaatcgccg ccgccgatgt gcgcaagtcg gttcaattct gccgtaaaat
    12181 tgatctgccg atccttggaa ttctggaaaa catgagcggc tatacctgcc cgaagtgcgg
    12241 agaaactgtt catctgttcg gcaaagacgg cggattcaaa acagcggaaa aatataacat
    12301 cgcgtttctc ggccgcattc cgattgatcc gaacgtcgga atctgcggcg atgccggaca
    12361 aagcttcctg cagaaacaca gcggctctgc agccgcgcag gcgtttgaaa ccgcagtgga
    12421 gcaaatcggg gtcgcagtac ggggatcttt ttcatgaccc agataaaaat tacagttttg
    12481 gtggagaatg ctgccggaaa acgcgatctg ctggcagagc atggactttc gtttttgatc
    12541 ggaagcggcc ggaataaagt tctcttcgat accggtcagg gactggctct acgacacaac
    12601 accgcaacac tcaacgttga tctgaaagcg atcaatgctg tcgtattgag tcacggccac
    12661 tacgaccaca ccggcggact gacctttgct ctgaaccgca tgaactgccc gcgtctttat
    12721 gtccacccgg aagccttctc cttgaaatac gcccgcaacc cggacggctc tgcccgcgag
    12781 gtcggcatga cggccggcaa taaaatcacg gcgggtgaac tggcggaaat tgttttgacc
    12841 gacgaaagaa cagagattgt cgacgggctg tttttgaccg gcccggttcc gcgcatcacg
    12901 gattttgaag ataccggcgg agcatttttt aaagaccagg aatgcaggaa ccctgatgag
    12961 ctcccggacg atcaggccgc attcatggaa acgaaaaacg gaacggtcgt gattctggga
    13021 tgtgcccact ccggagttat caatacgctg agatatattc agactctcac tcataaccgc
    13081 ccgattcata ccgtcatcgg cgggatgcat ctactccatg ccagcccgga gcgcatggat
    13141 caaactgtcg ctgaactgcg ccgattaaat attcaacgcc tgatcccctg ccactgcacc
    13201 ggattcgccg cgatggtgcg cttatggaaa gaatttccgg gcaaatgcga aacatgcccg
    13261 tccggtacag tcatacaaat cgaggattaa actgaaaatg ataaaacttt atacgagcaa
    13321 acactgcccg atctgcgaac aggttaaagc tgcattaaaa ccaatttctc atcagattct
    13381 gtccgcagag gatcatatgg atgacttgat caaagaagtc accgaccgga aactggatca
    13441 ccgacaaatc cgtcaggctc cagtgatggt ggtccccggt tgcgacctca tctggaccgg
    13501 agccgactgc ctgatcgcga tcgaagaaaa agaatgggag taaatctgga caaaaaacgg
    13561 ttcattccgt ctgcttttca ccgactgatg cgtacagaac cacccggctg aaaatccgcg
    13621 aaattgccga acgcctttat aaaacatgtc aaacccgcag ggaacccgaa tggttccttg
    13681 cacaaaaagg agaacccgaa tgaagatagc cattcccgta acaaatgaga aattatgtat
    13741 gcacttcggc cattgcgaaa ccttcgccgt gctgaccgtt gacgaatcgg ccaaacgaat
    13801 caccggacga caagacatgg ttccgccgcc gcatgaaccg ggggtcctgc cccgctggct
    13861 cggcgaacag aaagtaaaca tcatcatcgc tggcggtatg ggccagcgag cacagcagct
    13921 ttttgcagaa cagaatatcg cagttgttgt cggagcaccg gcagaaacac cagaaacgct
    13981 ggttacagac tatttggccg gaacattgga atccggcgca aacgtctgcg atcattgatc
    14041 aacgggttac aaaccccgga aaagtttccg gggtttggac attcatgagc tggtttatgg
    14101 gatttacagt gaaacggttt ccgtttatga atggggccgt tgcagccggg aacgcgtccc
    14161 gcgcaaagaa aagcgaaagg aacagcccat gaaaagactg agttttgccc ttcccctgct
    14221 tgtgctggcc gccgtcatgg ccggatgcac ccacccgatg accgtcacca acatggacaa
    14281 ctatcacaac agaaccatcg tttcgctcga cgaacgcctg cgcatcggca tccgcgccaa
    14341 cgccgccgac ctcaacggac accgcatgat ctacgccatc ggccgcgacc tgctcaaata
    14401 taacgcgcag agcaccacgg cgatcgaaga gaccaatgaa tatgtcgacg tcatcgccaa
    14461 tatctccgtc gtttccaagc agcgcgggtc cggatggaac ttctgggccg actttcccgg
    14521 cttcctgatc tggttcccgt cgtggcacgg ctacaactac gacgtcatgt atgacatcga
    14581 cgtaacgctc aacgacgcca gaaccggcaa actcattaac agcatgtata tccccgtccg
    14641 gttcgacgtg aaacacgccg acatcagccg cacctgggtg gacggcgtcg cctggcccac
    14701 tctgggcgta agcgcgctga tcggcggact tcataacatg acctacgacg aaaccgtcac
    14761 cccgctcgtc gaacaggacg ccacgccgat tctggccgac tacgtcgccc agcagatcgc
    14821 attcaccctg cactactaca aatccgctcc gcgcgaccgc tttgaaaagc tgaaaatgct
    14881 gctggatgaa aaactcatca gccaggaaga gtacgacatg aaacgcaaag ccgtcatcaa
    14941 cgacatctaa accgcttcat ctgcacgctg aaagcgctcc ggtttccgga gcgctttttt
    15001 tatgcccggg gatgaaactg acggtgaact cccttgagac ggtcgggatc gacgtgcgta
    15061 taaatctggg tcgtggcgat gtcggcatgg ccgagcatct cctgaataat gcgcagcggc
    15121 gcgccgttgg ccagcagatg gctggcgaat gaatggcgca gcgtgtgcgg atagatggtt
    15181 ttggtaatcc ccgcagcacg ggcgcacttt ttgatcatat cccagatcgt tttgcgcgac
    15241 atcggctgac ggcggctgct caggaaaacc gtctgctcct gcggcccggg cgtaagctgc
    15301 ggacggactt ctttgatgta ctgcttcacc ttttcgatgg cttcttttcc gaccggcacc
    15361 acgcgttcct tgcgcccttt gccgacacag cgcaaaaagt gctcatcgaa atgcaggtcg
    15421 tgcagctgca gaccggccag ctcggaaacg cgcagaccgg aagcatagaa aatctgcaat
    15481 atggcaaggt tgcggatgcc cagcggcgtt gtcagatccg gagcggcaaa aagacggtca
    15541 atctctttgg gcgtcagtac gctcggaagc atcttccaga ctttgggcga ctccatcgtc
    15601 tcggcaacat tgatgctgag cagcccttcc tgcacgaggt agcggaagaa aaccttgagc
    15661 gaaacgagat ggcgcgaaag cgagttggcc gccagaccgc gctctttttc cgccatcagg
    15721 tggtcgagaa tctgcttacg cgtcacttca ttgatggaac ggacgccttt ttgcgacagc
    15781 cagtcgagga atcgcccgat atcgtcggca taggccgcac gagtattcgg gctcagcccc
    15841 atttcgagcg aaatgtaatc aagaaactgt tccagcagtg cccgcataaa gaggatatta
    15901 accactaatt ttcactgatc cccactaatt agtgttcatt agtgaaaatc agcggatcgt
    15961 attcttaaag cttggcacct gtgagccgct cataagcttc aaggtatttg gcctgagtct
    16021 tgacgataat ttcttcgggc agcgcgggcg caggcggagt tttgtcccag tcgagtgttt
    16081 cgagatagtc gcggacaaac tgtttatcat acgacattgg cgacgagccg actttgtagg
    16141 tgtcggcggg ccagaagcgc gagctgtccg gcgtgagagc ctcgtcgatc agaatgattt
    16201 cgccgtcgag ttcgccgaac tcaaatttgg tgtctgcgat aatgatccct ttggtcagcg
    16261 cgtattcagc ggcccgtttg taaagggcga tgctgatggc tttcagcttt tcagccttcg
    16321 ccgcgccgac aatctccttg gccttttcga aggagatgtt ttcgtcgtgc agtcccattt
    16381 ccgctttggt ggacggggtg aaaatcggtt cgtcgagttt atcggccatt ttatagccgg
    16441 cgcgcagcgg cattccgccg atggtgccgc tctgtttgta ttccttccag ccggaaccga
    16501 tcaggtagcc gcgcacaatg cactcgaccg gaaacacggt ggttttttta accagcatgg
    16561 aacgtccggc aagctggtcg gcgtgctgat taaacggcgc agggaatttg ctgacatcgg
    16621 tggtgatcat gtggttcttc acaatgtcgc cggtgcgctc gaaccagaac ttggaaatca
    16681 tattaagtac gctgcccttt ttcggaatgc cgttcggcat cacgcagtca aaggcggaaa
    16741 tgcggtcgct ggccacaaac agcagcttgt cgcctgcgtc ataaatttcg cgcaccttgc
    16801 cgctgcgcgg cgcaggaact ccgggaatct gaatcttggt taaagcctgg ctcatcgtct
    16861 ctcctgaggt taattcacaa agggtgaaag ttcacgcaga cgttttacaa ctttcggcag
    16921 ttcggtcaat accgcgtcta catcttcgtc cgtattatac gtgctgaaac tgaaccggat
    16981 ggaaccgtga acagaaacgc ccgctacatt catggcgcgc agaacgtgcg acggctccag
    17041 cgagccggag gcgcaggccg agccggtcga agcgcagata ccgcggtcgt cgagcatcag
    17101 cagaatggct tcgccttcga tgaagtcgaa gctgatgttg gttgtgttgg gcagacggca
    17161 tttcggatcg ccgttgacgc gggccccggg gcacgtcgcc agaatgccct gctccagccg
    17221 gtcgcgcagg gctttgacgc gcgtgttttc ctcgttgagg tgtttacccg ccagatcgca
    17281 ggcgacgcca agaccgacaa tatacggcac gttttccgtg ccggcacgcc gtccgcgttc
    17341 ctgatggcct ccgagcacca gcggcttcac cttggtaccg cgccggatgt agatggctcc
    17401 gacgcctttg ggtgcgtgca gcttatggcc ggacagtgaa agcatatcga ccggcaggtc
    17461 gctgactttc atcggaattt taccgaccgc ctgtacggcg tcggtatgaa aaatgccgcc
    17521 cgcttcatgc accatctcgc cgatcagtcc gaccggaaaa attacgccgg tttcattatt
    17581 ggcccacatg atggaagtca gcgttttaga gctcttcact tcgcgccgca aggcgtccag
    17641 atcaaggcgt ccgagctgat ccacgtcgat cttggtcagg cggaaaccgc ggtcttcgag
    17701 gtagtggcac gggccggaaa ccgcggcgtg ctccactttg gaagtgataa catgcggcgg
    17761 cttgtccatc gcttcagctc cgccgcgaat ggccagattg tcgctttccg aaccgcagct
    17821 ggtgaaggtg atttcctgcg gatcggccgc gccgatcagc gcggcaacct gttcgcgggc
    17881 ggcttcgaca tattttttaa tctgcccgcc gaaggtatgc atactggacg gattgcccca
    17941 cagatcggtc agaaaaggca tcattgattc gcgtacttca tccgcgattt tcgtcgtggc
    18001 attgttatcg agataaaccg tgcgcattat ttgacctcct cgacccggat atcttcggaa
    18061 acgttttcct tcaggcgggc ttcaaccacg tccttcagcg taatcgccga aacgacacag
    18121 ccggagcagg cgccgcgaaa gcgcacttta accgtgtctc cgtccacatc aatcagttca
    18181 aggtcgccgc cgtcctggcg cagcgccgga cggatttccc ggtcgatcac atcttcgatc
    18241 aaatggatct tctgcagatt tgtcagcggt ttcttttcag gaacagcaga cgtcgcggcc
    18301 atttcgccgt gaacgcggtc gatgattttc tgaagttcgg gcagacagtt tccacagccg
    18361 ccgcccgctt tgcaggcatt ggtcagctct tcgagcgtct ggacattgtt ttcgcgggta
    18421 acgcgctcaa tctctttatc cgtcacgccg aagcagtggc agatcacttc gccttcgaga
    18481 tccttgtcga atttttttcc ggtgcggtag ttatagatgg ccgcttcgag cgcttcgcgc
    18541 cccatcacag agcaatgaac tttctgtttt ggcagaccgc caagatactc ggcgatgtcc
    18601 ttgttggtta tctgttccgc ttcttcgagc gttttaccga tgatcatttc ggtcagcgcc
    18661 gaggaggacg caatggcgct ggcgcagccg aatgtctgga atttcgcttc tttaatgcgt
    18721 ccggtttcat ccagcttaaa catcagcttc agcgcatcgc cgcacgccag tgagccgacc
    18781 tcgcctgtgc cgtccggttt ttctattttt ccgacgttcc gcggattttt aaaatggtcg
    18841 ttcactttgt ctgtatagtc ccacatggca attctcctga aatatgacca tagtaatcgg
    18901 gtttaaaggt tatggtcaac cgatttcgag catccgttca atcgaaccga ctgcgcgccg
    18961 ggcggtttcc gccggaaccg tcacccgata tttcatatcc tgaagcgacc agagcacctt
    19021 ttcgagcgtg atcttcttca tattcg
//
LOCUS       DAOPZO010000021        18895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_3053_length_18895_cov_10.183917, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000021 DAOPZO010000000
VERSION     DAOPZO010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 18895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18895
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3053_length_18895_cov_10.183917"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /gene="galE"
                     /locus_tag="PKI68_02705"
     CDS             complement(<1..716)
                     /gene="galE"
                     /locus_tag="PKI68_02705"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNX52635.1"
                     /translation="MKVLVAGGAGYIGSVTTEMLCDAGHDVTVFDNLERGHRAAVDPR
                     AGFIQGDLRKPETIEAALKSVKPEAVIHFAAYIEVGESMVNPLPFFENNVSGSLNLMK
                     AMVAADCKKLIFSSTCATYGTPERVPMDETLPQKPESVYGESKLLCEKIFSWAQQIHG
                     VECVFLRYFNACGATARYGEDHAPESHLIPLILQVPLGKRQKIYIFGDDYETPDGTCI
                     RDYIHIRDLAQAHILALKPG"
     gene            961..3987
                     /locus_tag="PKI68_02710"
     CDS             961..3987
                     /locus_tag="PKI68_02710"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNX52636.1"
                     /translation="MLYATALLILIAGISAARYAGRQTDLQMRRDLVNRTAAIALAID
                     PEDIRSLSFSSADLNRPEFLRLRGHLRAVAEASGLRSLYTMALRNGKIVFGPESLPPD
                     DRYASPPGTVYQTPTEKDFEIFRTGEPQIQGPARDEYGTFVTATFPVTDPQTGETLLV
                     AGIDVEASAWRAAIRKAQWIPILIAAGLLSILLAGRLLIKWRTNHSPAPAGHLRHIES
                     SLCAVFMLMLTLTVSVLLNNAEKKAREESFDTLARAQAAIFNETFIRFHNELHAVSQF
                     FESSELVTREEFQQFERRLTTDTPALACIWIPEVPAGEAEPFTAAARKDGLNGFRIWQ
                     QDEQGNAIAAAGRPLFYPVFYIEPSGHTEKAYGYDIGSDPLCRAAIQETLRTGLETAT
                     DPGKLIALPDQPCGIFIFQPVSAGRQKGLTGLAVDIEQMIRNRFYKTGGDHAGLSVTL
                     YQLQSGEKPRRLVCSKPNCSETCHENKKYGLYETVPLFAFGKTYSLLILPEKKWLDAH
                     PLSSGRNAAAAGILLTVLLCGLVHMLANRHLILERLVQERTDELDRSRRKIASLFRSA
                     PVGIGLAVDRVLMEVNDQVCTMTGYARSELLGSDALLLYTSQEEYERVDRERSEQISK
                     TGSSTIETRWKNRDGTVTDILLTSAPIDPADLQKGITFTALDISRRKQDEQALRASEE
                     KYRTTFSSIGDAVISADVTERIAGMNSAAEALTGWKESEAVGRPLATVFRIINETTRN
                     PVESPAAKVLAEGKIVALTSHTVLLCRDGREIPIADSGAPVKNEKGEITGIVLVFRDQ
                     TRERSVQKVIEESELRYRQLFENMTAGFALHEMIYDRLGAPQDYRFLVVNPAFERLTG
                     LSADRIIGRTVKEVLPGTELRWIEQYSKVVKTGVAAEFQDYSKDLNRFFDVKAFCPAP
                     GRFAVVFTDITKQKHAEADLNERLSELERFNRAAIDREVRMVALKKEINALSTQLNRP
                     APYPVASAKPEPSVPPGPERNGFFKQLFKRFTSHAN"
     gene            3977..7495
                     /locus_tag="PKI68_02715"
     CDS             3977..7495
                     /locus_tag="PKI68_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNX52637.1"
                     /translation="MQINRFFSLLLVCAAPLCRAETDIRSAAEPNYPPLSVVNSDGHA
                     DGFAVEMLRAALKEMGRNVVIDSGSWQDIKQQLADGKIDVLPLAGRTPEREAVFDFTI
                     PYLTLHGALFVRDDSTGIHTMDDLKDKRIAVMRGDNAEEYIRRANLSEHILTTPDFEE
                     AFRMLSDGRADAVVAQKLMGITLLKKLNITNVRITGKPNQEFRQDFCFAVKSGNKKLL
                     SVLNEGLALITANGTARQLQQKWLGRDERTLALARTIIYGGNLDFPPYEFLDEKGRPA
                     GFTVELTRAIARELGVNIAIELAPWSETVRKLEHGELDVSSMQYTPSRGRQFSFSTPF
                     STMYQAIFARNDAPPYRRFGDLSKYRIAVRNASVMYDYALRQGLSNSLVITERPEDAL
                     HLLIENKADYALLPYWQGVQEAEKNGWKNLHAVEKRLTETEYCYTTAAGNTELINLLN
                     DGLARLKESGEYRNIYNKWMGVLEPDSRREQTKKILMAFLAFFALPAMAGGGIIFALN
                     RTIKRRTAELELANRTLNDSRLAALNMMEDAIEAKNQLEESQTRLRLTQFSLDHSGDA
                     VFWVDSAERFFYVNEAACRSLGYTRDELLQLSVKDVNPGFPQEKWPTFWEKLKEKESL
                     VFEACHKTKTGQTVPVEIHAGFITFGSLECICAFVHDISDRKRREAELQRLSTAIGQS
                     PEAVMITGPDGIIQYVNPAFETISGFSRQEAIGKNPRILKSGQHDAVFYSNLWKTIGS
                     GRNWEGRFINKRKNGKLYTEEATVSPVRDSSGNITGYVAVKRDITEELGKEEQYRQAQ
                     KMEAVGQLAGGIAHDFNNILQAILGFSEILLTRLASDTVEYRNVDEIQKAAKRAAELT
                     RQLLAFSRKQPAEKKLLQLNSTLQDTEVLLQLLLGEKVRCSLKLTPDLHPVYADHGHI
                     TQIILNLAVNARDAMPDGGLLTISTENILFTEQNVPAHPDARQGNFICLSIADTGTGL
                     SPEVKDHLFEPFFTTKGIGKGTGLGLSVIYGIVKQSKGWIQVESEEGRGTVFKIYLPA
                     CEPGALSADSHVSPDGSTDQRILLVEDDPAMRDMVLRLLQNAEYQTFSAGNLKEAERI
                     FEQERGRFDLLFSDIVLPDGNGVELADNLRKKKPGFPVLLYSGYQNQRERWDNLDSKG
                     YHFLQKPFTVTALLAMVHDILTEAKK"
     gene            7536..7907
                     /locus_tag="PKI68_02720"
     CDS             7536..7907
                     /locus_tag="PKI68_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX52638.1"
                     /translation="MGNLLIIEDDLSVQQMFKQFLESNGFTVFCASEGLEGMNLLKQQ
                     KVDLIITDIMMPEMDGLEVIQAVQETQPGLPIIAISGGMRDVPINFLPHARKFGACRV
                     FEKPVSLTTLLASVQELLKTE"
     gene            complement(7914..8870)
                     /locus_tag="PKI68_02725"
     CDS             complement(7914..8870)
                     /locus_tag="PKI68_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52639.1"
                     /translation="MKMKTGFFALSTLILFAAGCKTTETKPVPWKKQSAYLAKVRDPR
                     VCGFDIYQTPKGIEYRGGSRVHANQGAVLPMKAEEPSRPVVMMSGKFGLATPVLLDFS
                     TPLSWLEFDLAGDLGATPVSERDAQLVKMPGDEFASCVSAVPAMKFKSLLIEFPLVHT
                     RMATGPLGQPARGIEKPELKGVVGWEQLRKFEQIQLDYSKKQVLLSTAKNIYSPDPAQ
                     VIARIPLVKYVGACTVRGAVNGKESLILIDPAGDFEVATDDASPVASMQLDADILFSA
                     PAVVKSSGGTRIGAQLLSKYRITICPQAGFIYFEKPDAGKTE"
     gene            complement(8870..9433)
                     /locus_tag="PKI68_02730"
     CDS             complement(8870..9433)
                     /locus_tag="PKI68_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52640.1"
                     /translation="MMRRWIIYALAALLALSVFGWIFYIPSEALCALKPVPAHAQLIY
                     RGETPGQFLSFFPLLGRTGSDDPGRWRKSFQMLENSPLTVATVPLGGRDRRDSWIAVS
                     TIGPRAFLLRWQLALFPPEGIKTARSYGAWPVWQYSDPSLPPWMRVRFSITEGLLICS
                     ISDDSHDIYYLLDTLDGRRPSAERKER"
     gene            complement(9430..>10320)
                     /locus_tag="PKI68_02735"
     CDS             complement(9430..>10320)
                     /locus_tag="PKI68_02735"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNX52641.1"
                     /translation="IGSLEMKNPVTVASGTFGYGPEYADLVDLDRLGAITVKGICTEP
                     HVGNSTPRTFETSGGMLNAIGLPGPGAQGFIKKYLPFFDNYRVPLIVNIWGKTVEDYS
                     RVAEIFNEQARISALEVNVSCPNVKEGGALYGTDVNLFRTVVDAVRARTQKPLIIKLA
                     PNVADIKAFARAAEECGADAVSIMNSYPAMAINIETRKPQLANRTGGLSGPAIKPIAI
                     KLVWEAAKVIKIPIIGMGGIYSPEDALEFVIAGATAVAVGTASFTDPSTAVRVIEGME
                     TWMAAKGIGSIAELKGTVQA"
     assembly_gap    10321..10420
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10498..11283)
                     /locus_tag="PKI68_02740"
     CDS             complement(10498..11283)
                     /locus_tag="PKI68_02740"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNX52642.1"
                     /translation="MTQEKTTVTEHRIFQGDYRLLRLSAPAVGPLVQPGQFVHVRVPR
                     LADAVLRRPFSVFKADSEGIAILYKSVGKGTAAMCSVREGEEVDLLGPLGTGFPMIGE
                     TTKTPVLVAGGYGNAALYLQAAKLPVKGVAFFGGRSAMDILCVNEFKELGWDVRVTTD
                     DGSLGQKGLVTAALDPWIAEQKNLKTVELFACGPNAMLKAVGDRAIRHGVTAWLSMDR
                     NMACGVGACLTCVIKRKTAEEWEWARCCKDGPVFEAREILWDE"
     gene            complement(11350..12333)
                     /locus_tag="PKI68_02745"
     CDS             complement(11350..12333)
                     /locus_tag="PKI68_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNX52643.1"
                     /translation="MKAFWFWISVVTVALVIFIGLCRLDGRPDATLHGAAAAENLKLI
                     QKQLAAGADINALDNDGLSPLMYAVKSGNPAAVDLLLNSGADVNLPGPQGNTAVHFAA
                     MQSNPRLVERLIAAGANLQVLNADQATPLCLAVRANSLAVEPLVAAGALTNGTVCAGQ
                     DYFLCAARSGNLKMINILIERGADVNVCASNGATALHYAVNRGHAEMVHLLLEHGANI
                     DVKDRYEWTPLHHAVRSDQVAIVRMLLEHGANTELRGYRGRTPLLMAAQLGRTGIFEV
                     LARGGADINTKSPEKETAEQLARDNRNPDILTFLEEYRRELKERRPGSPVQ"
     gene            complement(12453..13121)
                     /locus_tag="PKI68_02750"
     CDS             complement(12453..13121)
                     /locus_tag="PKI68_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX52644.1"
                     /translation="MSVTTPPGAKKKILIVDDHAFVRQGLRDFIERESDLAVCGEAVS
                     RAEALQRCTAATPHLVLIDLSLGGESGLDLVKDIAAQLPDIKTLVLSMQDEMLYAERV
                     LRAGAVGYVSKSAHPDRLVEAVRCVLGGGVYASEEVKQKIMQTVRRVDAGCDSITKLS
                     DRELAVFEQIGKGQSTADIAEAMHLSVKTVETYRARIKAKLGVHGSTELVQRAVQWIF
                     DKKV"
     gene            complement(13151..13957)
                     /gene="mazG"
                     /locus_tag="PKI68_02755"
     CDS             complement(13151..13957)
                     /gene="mazG"
                     /locus_tag="PKI68_02755"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNX52645.1"
                     /translation="MKETSIDRLLGIMRKLRAPDGCPWDREQTLDSLKSNLIEESYEV
                     IDAIESGDRAELRGELGDLLLQVVFQAQICEESGDFSFDDVVNGIADKLIRRHPHVFG
                     DVKAGTSGEVLRNWEKIKKTEKDAQTPRSLVEGIPRHLPALAKAHLVQKRVARAGFEW
                     DEIGGVIAKLEEELAEVKEAMAQKNAAAIREELGDLLFSTVNLSRYLGHESEDLLNEN
                     IAKFMRRFRSLENRLHAEGREIEKCSLEELDAVWNAVKADEKENRNKQEL"
     gene            complement(14023..14736)
                     /locus_tag="PKI68_02760"
     CDS             complement(14023..14736)
                     /locus_tag="PKI68_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52646.1"
                     /translation="MSKAKTETKPAAEKNKAREVLFFELEYVAVNGRQIMFDALKQVL
                     KSKDIEVTPALFARCGLTPRPGKAIQAMIEASGRNLTTGDQLTEQAETLMVKAFADKA
                     VLNKDLPALIKAAQERNIQVVALSAWPEPAAKELMKKTGLDTLGVDLVAMDSADPIFP
                     RADHWLRILKQRGQENIPLIAIVASRAACKGALTAGATAIALPDAYTAFEDFTGAKVV
                     LDTLGDLSAKELLELVSRR"
     gene            14928..16031
                     /gene="carA"
                     /locus_tag="PKI68_02765"
     CDS             14928..16031
                     /gene="carA"
                     /locus_tag="PKI68_02765"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNX52647.1"
                     /translation="MKKAIIALEDGTCFTGRAFAGAGEFYGELVFNTSMTGYQEILTD
                     PSYNGQIVTMTYPLIGNYGVNPEDVESRAVFAKGLVISECSRIQSNWRSTMSLPEYLE
                     KAGVVGVDQVDTRAITIHIRDKGAMKCVISTEDLDCASLVKKAKESAGLVGRDLSTEV
                     SAQKPYIFPEGGKPNARFRVAVIDCGIKTNQLRIFNELGCECHVFPNTSSAKEILACK
                     PDGLFLSNGPGDPAGVPQIVATVKELIESKLPTFGICFGHQMLGQAFGAKTYKLKFGH
                     RGANQPIQDLRTRKVEIASHNHGFCVDPATVDPEIAEVSHINLNDNTVAGLRHKTLPV
                     FCVQYHPEACPGPHDPYYLFEEFIALMEQYKKR"
     gene            16031..16222
                     /locus_tag="PKI68_02770"
     CDS             16031..16222
                     /locus_tag="PKI68_02770"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNX52648.1"
                     /translation="MKDLKNIILLTLGVLLMIVILQNTATVSFHVLLWKVSMPLIIMT
                     AVSLFAGFAAGRLTRRKRG"
     gene            16224..17771
                     /gene="guaA"
                     /locus_tag="PKI68_02775"
     CDS             16224..17771
                     /gene="guaA"
                     /locus_tag="PKI68_02775"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF000848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNX52649.1"
                     /translation="MKHSEWIAILDYGSQVTQLIARRIREQKVYCEIIRFDTKAADLA
                     GRAPKGIILSGGPASVTDQGSPLCDPAIFDLNIPILGICYGMQLTSQTLGGKVHRGTK
                     REYGKATMQVTKECPIFQGLEKNLQVWMSHGDKVEALPAGFEAVATSDNCPYAAMQNA
                     AKKIYGVQFHPEVVHTPQGAQMLGNFAYKVCGCSGDWKMSKFIEDNIAAIRAKVGSDH
                     VLLGLSGGVDSSVVAALLHKAIGPQLHCVFVDNGLLRYNEAREVEELFGNAFGIDLHT
                     AHAQDIFLNALAGVSDPEKKRKIIGKTFIDVFAEKARSLSGKVKYLAQGTLYPDVIES
                     VSPIGGPSATIKSHHNVGGLPADLKFDLIEPLRELFKDEVRAVGRELGLPSYVVDRQP
                     FPGPGLAVRILGEITKDRIDLLQLADLRVREEILKMENHLDVWQYFAVLLPIQTVGVM
                     GDDRTYENVVAVRAVQSLDGMTADWYKLPYEVLDSISNRIINEVKGVNRVCYDISSKP
                     PSTIEWE"
     gene            17808..18173
                     /locus_tag="PKI68_02780"
     CDS             17808..18173
                     /locus_tag="PKI68_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNX52650.1"
                     /translation="MGSEFDRCDLKERTTAFAIAIVKLYSALPKQDRYEVQIASKQMI
                     RSGTSVAANFREAARARSKADFISKIELCTQEADETLLWLEVLEKGCNLTTETIEALK
                     KETDELIAIFVTMSKNSKS"
     gene            18223..>18895
                     /locus_tag="PKI68_02785"
     CDS             18223..>18895
                     /locus_tag="PKI68_02785"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52651.1"
                     /translation="MPKRTDIHHILIIGSGPIVIGQACEFDYSGTQACKALREEGYRV
                     TLINSNPATIMTDPATADATYIEPITPETVEKIIIKERPDALLPTLGGQTALNTAMKL
                     YRSGVLQKYNVEMIGANYDAIMRGEERDIFKSVMKEAGIETLKSFHVHNLEEATRVAK
                     EELPFPIIVRPSFTLGGTGGGIANNMEELLQIARRGLEASLSSEVLLEESVYGWKEFE
                     MEVMRD"
BASE COUNT         4352 a         5427 c         5117 g         3899 t
ORIGIN      
        1 atgccgggct tgagcgccag aatgtgcgcc tgcgcaaggt cgcgaatgtg gatgtagtcg
       61 cggatgcagg ttccgtccgg cgtttcgtag tcgtcgccga agatgtaaat tttctgccgt
      121 ttgccgagcg gcacttgcag gatgagcgga atcaggtggc tttccggcgc gtgatcttcg
      181 ccgtaccggg cggtggcgcc gcaggcgttg aagtagcgca ggaacacgca ttcgacgccg
      241 tgaatctgtt gagcccatga aaagattttt tcgcagagca gcttggattc gccataaacg
      301 ctttccggct tttgcggaag tgtttcgtcc atcggaaccc gctcgggtgt gccgtaggtg
      361 gcgcaggtgg aggagaagat cagttttttg cagtccgccg cgaccatggc tttcatcagg
      421 ttgagcgagc cgctgacgtt gttttcgaag aagggaagcg ggttgaccat cgattctccg
      481 acttcaatgt aggcggcgaa gtgaataacc gcttcgggtt tcaccgactt cagtgccgcc
      541 tcaatggttt ccggtttgcg caggtcgccc tggatgaatc cggcgcgggg atcgactgcg
      601 gcgcggtgac cgcgttccag attgtcgaac accgtcacgt cgtgccctgc gtcgcaaagc
      661 atctctgtgg ttacacttcc gatatatccg gcaccgccgg cgaccaaaac cttcattaaa
      721 aatctcctgc gttaactgaa accgagccgg aaagatagcg ccggatgatc gcttcgccaa
      781 gccattcctc tgcgtgctgt ttcatattga gcctgtccgg gaaatacaat agggttccgc
      841 cgtcatattt gtgactgccg gccggcggaa tccggattga cagtaaaggc tgaaaccatg
      901 aacggaaagc ggcgcgcagt tttcttgccg aaaaatccag ccgggcacag aagtaattat
      961 gtgctttacg caaccgcact gctgatcctg attgccggta tcagcgcggc gcggtacgcc
     1021 ggccggcaga ccgatctgca aatgcgccgg gatctggtaa accggaccgc cgccattgcc
     1081 ctcgccatcg acccggagga tatccgttcg ctttccttct cctctgccga cctgaacagg
     1141 cctgaattcc tgcgtctgcg cggtcactta cgcgccgtgg cggaagcctc cgggctgcgc
     1201 agtctttata cgatggcgct gcgcaacgga aaaattgttt tcggcccgga aagtctgccg
     1261 ccggacgacc ggtatgcttc gccgcccggc acggtatatc aaactccgac tgaaaaggac
     1321 tttgagattt tccgtaccgg tgaaccgcag atccaggggc ccgcccgcga tgaatacgga
     1381 acgtttgtaa ccgctacctt tccggtaacc gatccccaga ccggagaaac gctgctggtg
     1441 gcgggaattg atgtggaagc ctccgcgtgg cgcgccgcca ttcgtaaagc gcaatggatc
     1501 ccgatcctga ttgcagcggg actcttgtcg atactgctgg ccggtcgttt gcttataaaa
     1561 tggcgcacaa accattcgcc cgccccggcc gggcatctgc ggcatatcga atccagtctg
     1621 tgcgcggtgt ttatgctgat gctgacatta accgtgtccg tactgctgaa taatgcggag
     1681 aaaaaagcgc gggaagaatc cttcgacact ctggcgcggg cgcaggccgc cattttcaac
     1741 gaaacgttca tcaggtttca taacgagctg catgcggtgt cgcaattctt tgagtcgagc
     1801 gaactggtta cccgcgaaga atttcaacag ttcgaacggc ggctgaccac agacacgccg
     1861 gcactggcct gcatctggat ccccgaggtt ccggcaggtg aagccgaacc gtttacggcg
     1921 gcggcccgca aagacggact taacggtttc cgtatttggc agcaggatga gcaaggaaac
     1981 gccattgcag cagccggccg accgctgttc tatcctgttt tctacattga accgtcaggc
     2041 catactgaaa aagcatacgg atatgacata ggatctgacc ccctttgccg ggccgcaatt
     2101 caggaaacgc tgagaacggg tcttgaaacc gccaccgatc cgggaaaact cattgcactg
     2161 ccggatcaac cgtgcggaat ttttatcttt cagccggttt ccgcaggccg ccagaaagga
     2221 ttgacgggac tggctgtcga tattgagcag atgatccgga accggttcta taaaacaggc
     2281 ggcgaccacg ccggactctc ggtcacgttg tatcaactgc agtccggcga aaagccccgg
     2341 cggctcgtct gctcaaagcc gaattgctcc gaaacctgtc atgagaataa aaaatacgga
     2401 ctttatgaaa ccgttccgct gtttgccttc ggcaaaacct attcgctgct gattcttccc
     2461 gagaaaaaat ggctggacgc acatccgtta agcagcggca gaaatgccgc cgctgccggc
     2521 attcttctca cagtgctgct ttgcggactg gttcatatgc tcgccaaccg ccacttgatt
     2581 ctcgaacggc tggtacagga gcgaacggat gaactggacc gcagccgccg aaaaatcgca
     2641 agcctcttcc ggtccgcacc ggtcggcatc ggactggccg ttgaccgcgt tctcatggag
     2701 gtgaatgatc aggtctgcac catgaccggc tacgcccggt ccgaactgct tggcagcgac
     2761 gcccttctgc tttacacctc gcaggaagaa tatgaacggg tcgaccgcga acggtcggaa
     2821 cagatcagta aaaccggcag cagtacgatt gaaacccggt ggaagaaccg cgacggaacc
     2881 gtgacggata tactgctcac ttccgcgccg atcgacccgg ccgatctgca aaaaggcatc
     2941 accttcacag cgctggacat ttcccggcgc aaacaggacg aacaggctct gcgcgccagt
     3001 gaagaaaaat accgcaccac attcagctcc atcggagacg cggtgatctc tgcagacgta
     3061 accgaacgta tcgccggaat gaattccgcg gcagaagcac tgaccggctg gaaagaaagc
     3121 gaagccgtcg gccggccgct ggccacggtt ttccggatta ttaatgaaac gacacggaac
     3181 cccgttgaga gcccggcggc caaagttctg gccgaaggaa aaatcgtcgc cctgaccagc
     3241 catactgtgc tgctttgccg ggacggccgc gaaattccga ttgccgacag cggcgcgccg
     3301 gtaaaaaatg aaaaaggcga aatcaccggc atcgtgctgg tcttccggga tcagacccgg
     3361 gaacgctctg tacagaaagt gattgaggaa agcgagctgc gctaccggca gcttttcgaa
     3421 aacatgaccg caggcttcgc gttgcacgaa atgatctatg acaggctggg cgctccgcag
     3481 gattaccgct ttctggtcgt caaccccgct tttgaaaggc tgaccggcct gagcgctgac
     3541 cggatcatcg gaagaacggt taaagaagtt ctgcccggca cggaactgcg ctggattgaa
     3601 cagtacagca aggtggtgaa aaccggcgta gccgctgaat tccaggatta ttcgaaagat
     3661 ctgaaccggt tttttgatgt aaaagcattc tgcccggcac cgggtcgctt cgccgttgtg
     3721 ttcacggata tcacgaaaca aaaacacgcc gaagcggatt tgaatgagcg cctgagtgag
     3781 ctcgagcgtt tcaaccgtgc cgctattgac cgggaagtgc gcatggttgc gcttaaaaag
     3841 gaaatcaatg ccctgagcac ccagttgaac cggcccgccc cctatccggt cgcatccgcc
     3901 aagccggaac cctccgtccc gcccggaccg gaacgcaacg gttttttcaa acagctgttt
     3961 aaaaggttta cctctcatgc aaattaaccg gtttttctca ctgcttcttg tctgcgcggc
     4021 accgctctgc cgggcggaga cggacatccg ttctgccgcc gaaccgaatt atccgccgct
     4081 gtcggttgtc aattccgacg gacacgccga cggattcgct gttgaaatgc tgcgggcggc
     4141 gctgaaagaa atgggccgga acgttgttat tgattccggt tcctggcagg acattaaaca
     4201 gcagctggcc gatggaaaga tcgatgttct gcctctggcc ggacgcacac cggaacgcga
     4261 ggcggttttt gatttcacca tcccctacct cacgctgcac ggcgcgctgt ttgtgcgcga
     4321 tgacagcacc ggcatccata cgatggatga tttgaaagac aaacgtatcg ccgtcatgcg
     4381 gggcgacaat gcggaggaat atatccgccg cgcgaatctt tcagaacaca tcctgaccac
     4441 gccggatttc gaagaagcat tccggatgct gtccgacggc cgggcggatg ccgttgtcgc
     4501 acagaaatta atgggcatta cgctcctcaa gaagctgaat atcaccaatg tccggattac
     4561 cggaaagccg aaccaggagt tcagacagga tttctgtttt gctgtaaaat ccggaaacaa
     4621 aaaactgctg tcggtgctga acgaaggcct ggccctgata acagcaaacg gtacggcccg
     4681 gcagcttcag cagaagtggc tcggaaggga cgaacgcaca ctcgctctgg cccgcaccat
     4741 catttacggc ggtaatctgg atttcccgcc gtatgagttt ctcgacgaaa aggggcggcc
     4801 cgccggattt acagtggaac tgacccgggc gatcgcccgc gaactcggcg tgaacatcgc
     4861 catcgaactg gccccgtggt ccgaaaccgt ccggaaactg gaacacggag agctggatgt
     4921 gtccagtatg cagtacactc cgtcaagagg ccggcaattc agcttttcga cgccgttttc
     4981 aaccatgtat caggcgattt tcgcccggaa tgatgcgccg ccctaccggc ggttcgggga
     5041 tttgagcaaa taccgtatcg ccgtccgcaa tgccagcgtc atgtacgact acgccctgcg
     5101 tcagggcctg agcaactcgc tggttatcac tgaaaggccg gaagatgccc ttcacctgct
     5161 gattgaaaac aaagccgact atgcccttct cccgtactgg cagggcgttc aggaagcgga
     5221 aaaaaacggc tggaaaaatc tgcatgccgt tgaaaaacgc ctgaccgaaa cagaatactg
     5281 ctacacgacg gcggccggga acacagagct gatcaacctg ctgaatgacg gactggcccg
     5341 gcttaaagaa agcggcgaat accggaatat ctacaacaag tggatgggcg tactggaacc
     5401 ggattcccgg cgggagcaga caaaaaaaat cctgatggca tttctggcct tcttcgcgct
     5461 gcccgcaatg gccggcggcg gaattatttt cgccctgaac cggacgataa aaagacggac
     5521 ggctgaactg gagctggcaa accgcacact gaacgattcc cgcctcgccg ccttaaatat
     5581 gatggaagat gccatcgagg caaaaaacca gctggaagag agtcagacgc ggctgcgatt
     5641 gacacagttt tctctcgatc actccggcga tgctgtattc tgggtcgatt cagccgaacg
     5701 gtttttttac gtcaacgaag ccgcctgccg ttcgcttgga tacacccggg acgaactgct
     5761 ccaactgtcc gtaaaagatg ttaatccggg attccctcag gaaaaatggc cgacgttctg
     5821 ggaaaaactg aaggaaaaag aatcgctggt ctttgaggcc tgccacaaaa caaaaaccgg
     5881 acagactgtc ccggtagaga tccacgccgg atttatcacg ttcggcagtc tggaatgcat
     5941 ctgtgctttc gtccatgaca tcagcgaccg gaaacggcgg gaagccgaac tgcagcggct
     6001 ctccaccgcc atcggacagt cgccggaagc ggtcatgatt accggtcccg acgggatcat
     6061 tcagtatgtc aatccggcgt tcgaaaccat ctccgggttc tcccggcagg aagcgatcgg
     6121 gaaaaatccg cgtattctca aaagcggaca gcacgatgcc gtgttttatt ccaacctctg
     6181 gaaaaccatt ggatccggca gaaactggga aggccggttt attaacaaac ggaaaaacgg
     6241 aaaactctac accgaagaag cgaccgtatc tccggtccgt gattcctcgg gaaacatcac
     6301 cggctatgtc gccgttaaac gcgatatcac cgaagaactg ggcaaagaag aacagtaccg
     6361 gcaggcgcag aagatggaag ctgtgggtca gctggccggc ggaatcgccc acgactttaa
     6421 caatatcctt caggcgattc tgggcttcag cgaaattctc ttaacccggc tggcatctga
     6481 caccgttgag taccggaatg tcgatgaaat ccagaaagcg gcgaaacggg cggctgaact
     6541 gacgcggcag ctgctggcct tcagccgcaa acagcctgct gaaaagaaac tgttgcagct
     6601 caactcaacc cttcaggaca cggaagttct gcttcagctg ctgctcggag aaaaagtcag
     6661 atgctctctg aagctgacac cggatctgca cccggtatat gccgatcacg gacatataac
     6721 gcagattatt ttaaatctgg ccgtcaacgc gcgcgacgcc atgccggacg gcggactcct
     6781 gaccatcagc accgaaaata ttctattcac agaacagaat gttccggcac atcccgatgc
     6841 acggcaggga aacttcatct gcttatccat cgcagatacc ggcacgggat taagccccga
     6901 agtgaaagac catcttttcg aaccgttctt tacaaccaaa ggtatcggca aaggaaccgg
     6961 tctgggactc tccgttatct acggcatcgt caaacaaagc aaaggctgga tccaggtgga
     7021 gagcgaagaa ggccggggaa ctgttttcaa aatttacctg ccggcctgtg aacccggcgc
     7081 cttgtcggca gacagtcatg tttcgccgga cggatcaacg gatcagcgca tcctgctggt
     7141 cgaagacgat cccgcgatgc gggatatggt tctccgtctt ctgcagaatg cagaatatca
     7201 gaccttttcc gccggaaacc tcaaagaggc ggagcggatc tttgaacagg aacggggccg
     7261 gttcgatctg cttttcagtg acatcgtcct tcctgacggc aacggagttg aactggccga
     7321 caacctccgg aagaaaaaac ccggattccc cgtcctgctt tacagcggct accagaatca
     7381 gcgtgaacgg tgggacaacc tggattctaa aggatatcac ttcctgcaga agcctttcac
     7441 cgtcaccgca ctgctggcca tggttcacga catcttgacg gaagcgaaaa agtaaggaat
     7501 tgtcaaactg cagccttaaa cgaaagagta ccgctatggg aaacctcctg atcattgaag
     7561 acgatctgtc cgttcagcag atgtttaagc agtttcttga aagtaacggg ttcaccgttt
     7621 tctgcgcctc tgaaggactc gaaggcatga atctgctgaa acagcaaaag gtcgatctga
     7681 tcatcaccga catcatgatg ccggaaatgg acggccttga agtcattcag gccgtacagg
     7741 aaacccaacc cggcctgccg attatcgcca tttccggcgg aatgcgcgac gtaccgatca
     7801 acttcctgcc gcacgccaga aaattcggcg cctgccgggt ttttgaaaag ccggtttctt
     7861 taaccaccct gctcgcttct gtacaggagc tgctcaagac cgaatagctt cggctattcg
     7921 gtcttaccgg cgtccggctt ttcaaaataa ataaatccgg cctgcgggca gatggtgatc
     7981 cggtatttac tcaacagctg cgcaccgatc cgtgtgccgc cggaagattt gacaaccgcc
     8041 ggagcggaaa aaagaatatc ggcatccagc tgcatggatg ccaccggcga agcatcgtct
     8101 gtcgcaacct caaagtcgcc ggccggatcg atcaggatga gactttcctt gccgttaact
     8161 gcaccgcgca ccgtacaggc gccgacatat ttgaccagcg gaatgcgggc aataacctga
     8221 gccggatccg gcgagtaaat attctttgcc gtcgagagca gcacctgctt tttgctgtaa
     8281 tccagctgaa tctgttcaaa tttcctaagc tgctcccatc cgacaacccc tttgagttcg
     8341 ggcttttcaa taccgcgggc cggctggccg agcgggccgg tcgccatgcg cgtgtgcacc
     8401 agaggaaatt caatcaggag agatttaaac ttcattgcag gcaccgccga aacacagctt
     8461 gcaaactcgt cgccgggcat tttgaccagc tgcgcgtcac gttcgctgac cggtgttgcg
     8521 ccaagatctc cggctaaatc aaactcaagc cagctgagcg gtgtgctgaa atcaagcagt
     8581 accggcgttg ccagcccgaa cttcccgctc atcatcacca ccggacggga cggttcttca
     8641 gctttcatcg gcagcacggc cccctgattg gcatgcaccc ggctcccgcc gcgatattcg
     8701 attcctttgg gggtctgata gatgtcgaat ccgcaaacac gcgggtcgcg cactttggca
     8761 aggtaggccg actgtttctt ccacggaacc ggtttcgttt ccgtggtttt gcagcccgca
     8821 gcgaaaagta tcaaagtact cagagcaaaa aaaccggttt tcattttcat cagcgttcct
     8881 ttctctccgc cgaagggcgg cggccgtcta atgtatcaag caggtaataa atatcatggc
     8941 tgtcatcaga gatcgaacaa atcagcagcc cttccgtgat ggaaaaacgg acgcgcatcc
     9001 acggaggaag cgacggatcg ctgtactgcc aaaccggcca tgcgccatag gagcgcgccg
     9061 tctttatgcc ttcaggcggg aaaagagcca gctgccagcg gagaagaaat gcacgcggac
     9121 cgatcgtgct gacagcgatc caggaatctc gccggtcacg tccgccgagc ggaacagtcg
     9181 ctacggtcag cggactgttt tccagcattt ggaaactttt cctccaccgg ccgggatcat
     9241 cgctgccggt tctgccaagc agcggaaaaa acgaaagaaa ctgtccgggc gtctcgccgc
     9301 ggtaaatcag ctgcgcatgt gcgggaaccg gtttgagtgc gcaaagagct tcagacggta
     9361 tataaaaaat ccagccgaac accgaaagcg cgagcagcgc ggccagcgca tagatgatcc
     9421 agcggcgcat catgcctgaa ccgtgccttt gagttccgcg atcgaaccga tgcctttggc
     9481 ggccatccat gtttccattc cttcaatcac gcgcacagcg gtggaaggat ccgtgaagct
     9541 ggcggtgccg acagcaaccg cggttgctcc ggcaatgaca aattcaagtg cgtcttcagg
     9601 gctgtaaatt ccgcccatcc cgatgatcgg aatcttaatg actttggcgg cttcccagac
     9661 cagtttgatc gcgatcggct taatcgccgg cccggaaagc ccgccggtcc ggttggccag
     9721 ctgaggcttg cgcgtctcga tattgatcgc catcgcagga taggaattca tgatcgagac
     9781 cgcatcggcg ccgcactctt cagcggcacg ggcgaaggct ttaatgtccg cgacattcgg
     9841 cgccagcttg ataatgagag gcttctgcgt tctggcgcga acagcatcaa ccaccgtccg
     9901 gaacagattc acatccgttc cgtaaagcgc cccgccctct ttgacgttcg ggcaggaaac
     9961 gttgacttcg agcgcactga tccgcgcctg ctcattgaaa atctctgcga cgcggctgta
    10021 gtcttcgacg gtttttcccc agatgttgac gatgagcggc acccggtagt tatcgaaaaa
    10081 cggcagatat tttttgatga atccctgcgc gccgggaccc ggcaggccga tggcgttgag
    10141 catgccgccg gaggtttcga atgtgcgcgg cgtggaattg ccgacgtgcg gctccgtgca
    10201 gatgccttta acggtaatcg cgccgagacg atccagatca accagatcgg catactccgg
    10261 cccgtagccg aaggttccgg aggcaaccgt caccggattt ttcatttcca gcgaaccgat
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agccgaaggt tccggaggca
    10441 accgtcaccg gatttttcat ttccagcgaa ccgattttta cacgcatgtc cggtcgttta
    10501 ttcatcccag agaatctccc gtgcttcgaa aacggggccg tctttgcagc agcgcgccca
    10561 ttcccactct tcggcggttt tgcgtttaat gacgcaggtc agacaggcgc cgacgccgca
    10621 ggccatattg cgatccatgg acagccacgc ggtgacgccg tgccggatgg cgcggtcgcc
    10681 gactgcttta agcatggcgt tcggaccgca ggcaaaaagc tccacggttt tcaaattctt
    10741 ctgttccgca atccacggat cgagcgcggc ggtgacaagg cctttttgtc cgagagaacc
    10801 gtcgtcggtt gtaacacgca catcccagcc gagctctttg aattcgttga cacagaggat
    10861 atccattgca ctgcgtccgc cgaagaaggc gacgcctttg accgggagtt tggcggcctg
    10921 aagataaaga gctgcgttgc cgtagccgcc ggcgaccagc accggcgttt ttgtggtttc
    10981 cccgatcatc ggaaacccgg tgcccagcgg accaagaaga tcgacttcct cgccttcgcg
    11041 cactgagcac atggcggcag tgcctttgcc cacggattta taaagtatcg cgatgccttc
    11101 agaatcggcc ttgaatacgc tgaagggacg gcggagaacg gcgtcagcca gtcgcggtac
    11161 gcgcacgtgg acaaactgcc ccggctgtac aagcggcccg acggcgggag cggaaagtct
    11221 gagcaggcgg tagtcgccct gaaagatccg gtgttcggtt acggtggttt tttcctgtgt
    11281 catcgggcgc ggattgtgcg gagtttcaaa tgcgaggtca aacgctttcc gcccgcggcg
    11341 atcaggttgc tattgaaccg gagaacccgg acggcgctcc ttgagttcgc ggcggtactc
    11401 ctccaaaaaa gtcaggatgt ccggattgcg attgtctctg gcgagctgct ctgccgtttc
    11461 tttttccggc gacttggtat tgatatcggc accgccccgc gcgagaactt caaaaatgcc
    11521 ggtacgtccg agctgtgcag ccatcagcag cggagtgcgc ccccgataac cgcgcagttc
    11581 cgtattcgct ccatgttcaa gcagcatacg gactatcgcg acctgatcac tgcgaacggc
    11641 atggtgtaaa ggagtccatt cataccggtc ttttacatca atattcgcgc cgtgttcgag
    11701 cagcaggtgc accatctctg cgtgccctct gttgacggcg taatgcagcg cagtcgcgcc
    11761 gttcgatgca caaacattca cgtctgcgcc gcgctcaatc agaatgttga tcattttcaa
    11821 attaccggag cgggcggcgc acagaaaata gtcttggccg gcacaaacag ttccgtttgt
    11881 aagtgctccg gcagcaacca gcggctcaac cgccaaactg ttggcccgca cagccagaca
    11941 aagcggcgtg gcttgatcgg cattgagcac ctgaagattc gcgccggccg caatcagccg
    12001 ttcgaccagc cgcggattgc tctgcattgc ggcaaaatgg actgctgtgt tgccctgcgg
    12061 cccgggcagg ttcacatcag ccccggaatt cagcagtaaa tccacggccg ccgggttccc
    12121 gcttttaacc gcatacatca gcggtgaaag accgtcgtta tcaagcgcat tgatgtcagc
    12181 tccggcggcc agctgttttt ggatcagttt gagattttct gccgctgcgg caccatgaag
    12241 ggtcgcgtcc ggacgcccat ccagccggca caacccgata aaaatcacca atgcaaccgt
    12301 aacaactgaa atccagaacc agaatgcctt caaagtatat ttctccataa actgttacac
    12361 ggcagagaga ttactctttt tgtgggatgc cgcatgtcag gcgttagcct gaaattatcg
    12421 tgagggttca gcctacaaag cagcaaaaag gatcacacct ttttgtcaaa aatccactga
    12481 accgcccgct gaaccagttc ggttgagcca tgcacaccga gttttgcctt gatgcgggcc
    12541 cgataggttt cgacggtttt gacgctcaga tgcatcgcct cggcgatatc cgccgtgctt
    12601 tggccttttc cgatctgctc aaagaccgcc agttcgcgat cactgagttt agtgattgaa
    12661 tcgcagccgg catcgaccct ccggacggtt tgcatgatct tctgcttaac ttcttcgctg
    12721 gcatagacgc cgccgccaag tacgcagcga acggcctcaa ccagccggtc gggatgcgcg
    12781 cttttactga catatccgac ggctccggcg cgcagaaccc gctccgcata gagcatctcg
    12841 tcctgcatag aaagaaccaa tgtctttata tcaggaagct gggcggcaat gtcttttacc
    12901 agatcaagac ctgattcgcc gcccagcgac agatcgatca gaaccagatg gggagttgcc
    12961 gccgtacaac gctgcagggc ttcggcccgg ctgacggcct cgccgcaaac tgccaaatca
    13021 gattcacgct caataaagtc gcgcagacct tggcggacaa atgcatggtc atcgacaata
    13081 aggatttttt tcttcgcgcc gggtggggtt gtaacgctca tgcggcgcat actgccagat
    13141 tcgccgctga ttacaattcc tgtttattgc ggttttcttt ttcgtccgct ttaaccgcat
    13201 tccagaccgc atcaagctct tccagcgagc acttttcaat ctcacggcct tcggcgtgca
    13261 gacggttttc cagactccgg aaacggcgca tgaatttggc aatgttctcg ttgagcagat
    13321 cttcggattc gtgtccgaga tagcggctga gattgaccgt cgaaaaaagc aggtcgccga
    13381 gttcctcgcg gatggcggct gcgtttttct gcgccatcgc ttctttaact tcggccagct
    13441 cctcttcaag tttggcaatg acgccgccga tctcgtccca ctcgaagcct gcacgcgcaa
    13501 cgcgcttttg caccagatgg gctttggcca gcgccggcag atggcgcgga ataccttcca
    13561 ccagcgagcg cggcgtttgc gcatcctttt cggttttctt gattttctcc cagttgcgga
    13621 gcacttcgcc ggacgtgccg gctttcacat cgccgaaaac gtgcggatgc cgccggatga
    13681 gtttatcggc aatgccgttg acgacgtcgt caaaactgaa gtctccggat tcttcgcata
    13741 tctgcgcctg aaaaacgacc tgtagaagta aatcgcccag ctcaccgcgc agctcggcgc
    13801 gatcgccgct ttcgatggcg tcgatcactt catagctttc ttcgatgaga ttggacttga
    13861 gcgaatcgag cgtctgctcg cggtcccacg ggcagccgtc cggcgcacgc aacttgcgca
    13921 taatgcccag caaacggtct atggacgttt ctttcatggc tctccaataa aaaaggctga
    13981 acacgatggt tcagcctttg tgctgcgaaa aggatgtccg tgttaccggc ggctgaccag
    14041 ctcaagcagt tctttggctg acagatcgcc cagcgtatcg agtacaacct tggcaccggt
    14101 aaaatcttca aacgcggtgt aggcgtccgg cagagcaatc gctgtggctc cggctgtcag
    14161 cgcgccttta caggcggcac gcgaggcaac aatcgcgatc agcggaatat tttcctgtcc
    14221 gcgctgtttg agaatacgca gccagtggtc ggcacgcggg aaaatcgggt cggcgctgtc
    14281 catcgccacc aggtcaacgc ccagtgtgtc gagtccggtc tttttcatca gttccttggc
    14341 ggccggttcc ggccaggccg aaagagcaac aacctggata ttgcgttcct gcgcagcctt
    14401 gatgagcgcc ggaagatcct tattgagcac cgctttatcc gcaaaggctt taaccatcag
    14461 ggtttccgcc tgctcggtca gctggtcgcc ggtggtcagg ttgcggccgg aagcttcaat
    14521 catggcctga atggctttgc cggggcgcgg agtaaggccg caacgggcga acagcgccgg
    14581 ggtaacctcg atatctttgc ttttcagcac ctgtttgagc gcgtcaaaca tgatctggcg
    14641 accgttaacg gcgacatatt cgagttcgaa gaaaaggact tcccgcgcct tatttttttc
    14701 ggcggccggt ttggtttcgg ttttggcttt gctcattcag tctgctccgg ttaattacac
    14761 atttttctat aaaagcgggg gatagtgtat gcggcggaac ccgcacaagt caagcgtact
    14821 aaaaatacct cccctcctgc gcaggaaaag ggttgcaagc ctcggtgtgg attgtgatta
    14881 tgtccggaat tttggcggaa tcacgggact ttttcagcgg acataaaatg aaaaaagcaa
    14941 tcattgcact tgaagacggc acctgcttca ccggccgggc ctttgccggc gccggagaat
    15001 tctacggcga gctggtgttc aacacctcga tgaccggcta tcaggagatt ctgaccgacc
    15061 cctcctataa cggccagatc gtcaccatga cctacccgtt gatcggcaat tacggcgtaa
    15121 accccgaaga tgtcgaatcg cgcgcagttt tcgcgaaggg actggtcatc agcgagtgca
    15181 gccggattca aagcaactgg cgctcaacca tgagccttcc cgaatatctc gaaaaagccg
    15241 gcgtggtcgg cgtggatcag gtggatacac gtgccatcac catccacatc cgcgacaagg
    15301 gcgccatgaa atgcgtcatt tcaaccgaag acctcgattg cgccagtctg gtcaaaaaag
    15361 ccaaagagtc cgccggactg gttggccgcg acctttcaac cgaagtcagc gcacagaaac
    15421 cttatatttt cccggaaggc ggcaaaccga acgcccgttt ccgcgtggcg gtcatcgact
    15481 gcggtatcaa aaccaaccag ctgcgcattt tcaacgagct cggctgcgaa tgtcatgtat
    15541 tcccgaacac ctcgtcggcc aaagaaattc tggcctgcaa gccggacggt cttttccttt
    15601 ctaacggccc gggcgacccg gccggtgttc cgcagattgt cgccaccgtg aaagaactga
    15661 ttgaatccaa actgccgacc ttcggcatct gcttcggcca ccagatgctg ggacaggcgt
    15721 tcggcgcgaa aacctataaa ctgaagttcg gtcaccgcgg cgccaaccag ccgattcagg
    15781 atctgcggac ccggaaagtg gaaatcgctt cgcacaacca cggtttctgc gtcgatccgg
    15841 ccaccgttga tccggaaatc gccgaagtca gccacatcaa cctcaacgac aacaccgtcg
    15901 ccggcctgcg gcacaaaacg ctgccggtgt tctgtgtgca gtaccatccc gaagcctgcc
    15961 ccggcccgca cgacccctat tacctcttcg aagagtttat cgcactgatg gagcagtata
    16021 aaaaacgctg atgaaagatc ttaaaaacat aatactgctg acgctcggcg tcctgctgat
    16081 gattgtcatt ctgcagaata cggcgacagt ttcttttcac gtccttctat ggaaagtcag
    16141 catgccgctg atcattatga cagccgtctc gctgtttgcc ggatttgccg caggacgcct
    16201 tacacgcagg aaacggggtt gagatgaaac acagtgaatg gattgccatt ttagattacg
    16261 gctcgcaggt cacccagctg attgcccgcc gcatccgcga gcagaaggta tattgcgaaa
    16321 tcatccgctt tgacaccaaa gccgccgacc tcgccggacg cgcaccgaaa ggcatcatcc
    16381 tttccggcgg ccccgccagc gtcaccgatc agggctcgcc gctgtgcgac ccggcgattt
    16441 tcgacctgaa cattccgatc ctcgggatct gctacggcat gcagctcacg tcccagacgc
    16501 tcggcggcaa agtacaccgc ggcaccaagc gcgagtacgg caaagcgacg atgcaagtca
    16561 ccaaagagtg cccgattttc cagggactgg aaaaaaatct tcaggtctgg atgagtcacg
    16621 gcgataaagt tgaagccctg cccgccggat tcgaagctgt cgccacaagc gacaactgcc
    16681 cgtacgccgc catgcagaac gccgcgaaga aaatctacgg cgtccagttc catcccgaag
    16741 tcgtccacac gccgcagggc gcacagatgc tcggcaactt cgcctataaa gtctgcggct
    16801 gctccggcga ctggaagatg agcaaattca tcgaagacaa cattgccgcc atccgcgcca
    16861 aagtcggcag cgaccacgtc ctgctcggac tgagcggcgg cgtggattcc tccgtcgtgg
    16921 ccgccctgct ccataaagcc atcggcccgc agcttcactg cgtcttcgtc gacaacggac
    16981 tgctccgcta caacgaagcg cgtgaagttg aagagctctt cggcaacgcc ttcggtatcg
    17041 atctgcacac agcgcatgct caggacattt tcctcaacgc gctggccggc gtttccgatc
    17101 cggagaaaaa acgtaaaatc atcggcaaaa ccttcatcga cgtcttcgcc gaaaaagcgc
    17161 gcagcctgag cggcaaagta aaatatctcg cacagggcac actttatccc gacgtcatcg
    17221 agagcgtttc gccgatcggc ggcccgtcgg ccaccatcaa gagccaccac aacgtcggcg
    17281 gactgcccgc cgacttgaag ttcgatctca tcgaaccgct ccgggaacta ttcaaagacg
    17341 aagtgcgcgc cgtcggccgc gaactcggcc tgccgagcta cgtcgttgac cgccagccct
    17401 tccccggccc cggtctggct gtacgcattc tcggcgaaat cacaaaagac cgcatcgacc
    17461 tgctccagct cgccgacctg cgtgtccgcg aagaaattct gaagatggaa aaccatctcg
    17521 acgtctggca gtactttgcc gtgctgctgc cgatccagac cgtcggcgtc atgggcgacg
    17581 accgcaccta cgaaaacgtc gtcgccgtgc gcgccgtgca gagcctcgac gggatgaccg
    17641 ccgactggta caaactgccc tacgaagtcc tcgacagcat ttcgaaccgc atcattaatg
    17701 aagtcaaagg tgtcaaccgc gtctgctacg acatttcgtc caaaccgcct tccaccatcg
    17761 aatgggagta aagcggaaac ctgaaccttg aaatttgaaa cctgaaaatg ggaagtgaat
    17821 ttgacagatg tgatttgaag gaacgcacca cagcgttcgc gattgccatt gtcaaactgt
    17881 actccgctct tccaaagcaa gaccggtacg aggttcaaat cgcgagcaag cagatgatcc
    17941 ggtccggcac atcggtcgcc gcgaacttcc gggaggcggc gcgggcccga agtaaagcgg
    18001 acttcatttc aaaaattgaa ctctgcaccc aggaagcgga tgaaacgctt ctgtggctgg
    18061 aagttttaga aaaaggatgc aatttaacta cagaaacaat cgaagctctg aaaaaggaga
    18121 ctgatgaact gatcgcgatt tttgtcacca tgtcaaaaaa cagcaaaagc tgatcgcgtc
    18181 atttcaggtt tcaggtttca agtttcaagt ttgaaagaca ctatgccaaa aagaactgac
    18241 atccaccaca tcctgatcat cggctccggc ccgatcgtaa tcggccaggc ctgcgagttt
    18301 gactattccg gaacgcaggc gtgcaaagcg cttcgcgaag aaggctaccg cgtcacgctg
    18361 atcaactcca atccggccac catcatgacc gacccggcca cggcggatgc gacctatatc
    18421 gagccgatca cgcccgaaac ggtcgaaaag atcatcatca aagagcgtcc ggatgcgctg
    18481 ctgccgacgc tcggcggaca gaccgcgctc aacaccgcca tgaagctgta tcgaagcggc
    18541 gtgctgcaga aatacaatgt cgagatgatc ggcgcgaact acgacgccat tatgcgcggc
    18601 gaagagcgcg acattttcaa gtcggtgatg aaggaagccg gcattgaaac cctcaagagt
    18661 ttccacgtgc ataatcttga agaagcgacc cgcgtcgcca aagaagagct gccgttcccg
    18721 atcatcgtgc gccccagctt tacgctcggc ggcaccggcg gcggtatcgc caataacatg
    18781 gaagagctgc tgcagatcgc cagacgcggg ctcgaagcca gcctcagcag cgaagtgctg
    18841 ctcgaagaat ccgtctacgg ctggaaagag ttcgagatgg aggtcatgcg cgaca
//
LOCUS       DAOPZO010000022        18409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_3168_length_18409_cov_10.436690, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000022 DAOPZO010000000
VERSION     DAOPZO010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 18409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18409
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3168_length_18409_cov_10.436690"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PKI68_02790"
     CDS             <1..222
                     /locus_tag="PKI68_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024731.2"
                     /note="among the AAA+ ATPases, the YifB protease family
                     belongs to the Helix 2 insert clade; unknown function;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease"
                     /protein_id="HNX52652.1"
                     /translation="AGMQPKHSRKYCRPTDDAAQLLKTAVSEMNFSARAYDRILKVAR
                     TIADLANKEQIGTEHIAEAIQYRTLDRMG"
     gene            complement(398..571)
                     /locus_tag="PKI68_02795"
     CDS             complement(398..571)
                     /locus_tag="PKI68_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52653.1"
                     /translation="MSEAASSSKETSESVRISSEVKQRLQEYCRQHKQPIRRVLDKAI
                     CDYVQDRQLKEER"
     gene            666..863
                     /locus_tag="PKI68_02800"
     CDS             666..863
                     /locus_tag="PKI68_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52654.1"
                     /translation="MSDTESVRISKELKQQLKQMAETMRPRTAIQYLIEDAIEQYLDR
                     AAEGNGPLYQVKPESKTKKKP"
     gene            900..1811
                     /locus_tag="PKI68_02805"
     CDS             900..1811
                     /locus_tag="PKI68_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52655.1"
                     /translation="MPETLYKTAGILLALLAMSLFNLAPVLQKSALDRLPPLLFSNLR
                     KSIISLLKDRRWMIGFIIGCLGLLPYVAAMSLVGVAVVQPLYGFGFIVLLVVSRFMLH
                     EKLHPAAKLAMGLLILMPVLIALGDVSGVQYDITRRSTQISLGVFVTITLLTSVVLIF
                     FTKKHPLYWAVISGALFGAGAVFSQLAITFLTADELRDLTGFTTIICNKNLLFCLAAV
                     LAATTVNVLAGYFMQVGLQQKMASQFMPITQTVNNAVAITGGVTLFGQCVTHWSFYIA
                     GIVMGLAGLFLLARFKHPSELDFKSGE"
     gene            complement(1795..3060)
                     /locus_tag="PKI68_02810"
     CDS             complement(1795..3060)
                     /locus_tag="PKI68_02810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX52656.1"
                     /translation="MKSWPQRPGHRVLILSVSAGTGHIRAGEALAKAAAAHPASGEVL
                     HIDALRYTNKMFRRFYGQFYIDLVKSAPTLLGWFYDSQDEPWKTEKMRLMLDRMNTGP
                     LVRQIKKFKPDITICTHFLPSEILSHLIKHGEIDTQLSITVTDYYAHAMWLSRLIHHY
                     FVATEESRVQLENIGFPPESITVSGIPIDPVFCEPKNRAALRAKQGLRNDIPMLLVSA
                     GALGVSSAEQIVAVLSELAVPVQIVVICGKNEKLRQMVLDQLSRRPDEASHLDFKVLG
                     YTTEMDEWMKSADLYIGKPGGLTTAEAMSCGLPMVIYQPIPGQEERNSDFLLENGAAV
                     KCNQLATMAYKVNLLLKDPERMAKMRRRALGLSHPDAASRIIQKLVDEIHTEAVIIPR
                     ESTGGKAKEKLRRLGWKLTPGSDDAIPRI"
     gene            complement(3057..3563)
                     /locus_tag="PKI68_02815"
     CDS             complement(3057..3563)
                     /locus_tag="PKI68_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52657.1"
                     /translation="MFFFRKKFHLIIVCTANRTRSAYFAGCLRHYLSQYRPDALRRIK
                     ITSAGTKAAGGGRINDVVALIARKQGFSLRQHMAEPLSGKLMKQADLILVMEQEHKDF
                     ILEKYPESKEKVFRLMEYGAQGDDAAETLDVPDPTGRRAEDFEAFIETAHAEVDRLVH
                     ELVYREII"
     gene            complement(3579..4241)
                     /locus_tag="PKI68_02820"
     CDS             complement(3579..4241)
                     /locus_tag="PKI68_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNX52658.1"
                     /translation="MKIHWLQHVPFEGLGCIAPWLAERRHAVTCTRLWAGDGFPAAEK
                     IDGLIVMGGPMGVYDEAVYPWLADEKAFIRQIVAQDKPVLGICLGAQLIAEVLGARVW
                     KNDQREIGFFPLAGDGALFPAEFTAFHWHGDTFGIPDGAVHLASSEACKNQAFSFKDN
                     VLALQFHLETTEESLMSLYQNCSGEITDGPFIQTLEQMRPFFPALEAANTRMFSLLKR
                     LF"
     gene            complement(4238..>4536)
                     /locus_tag="PKI68_02825"
     CDS             complement(4238..>4536)
                     /locus_tag="PKI68_02825"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNX52659.1"
                     /translation="KTLVNYADGAVVSRTLLKKETGNITLFSFDKGQGLSEHTSPFDA
                     VVEVVEGEGTFVIAGEPQTVKAGELIIMPANIPHDVQAADAPFKMLLIMIRSKA"
     assembly_gap    4537..4636
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4637..5292)
                     /locus_tag="PKI68_02830"
     CDS             complement(<4637..5292)
                     /locus_tag="PKI68_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX52660.1"
                     /translation="MKRKIIEIDEEKCNGCGACVTGCAEGALAVVNGKAKLVNDVFCD
                     GLGACIGECPTGALKIIERDAPEFSETAVQKHLQKTAQPSGGCPGMKLRFAEPAKVAK
                     SKIEGQGVSGQVIPSELNQWPTQLHLVPVEAPFFKNRELVVLSTCSPSASPDVNWRFV
                     RGRAIAIACPKLDRTEGYVEKLAAILSSNGIPKVIIVRMEVPCCGGLTMMVQEAARRS
                     G"
     gene            complement(5784..8612)
                     /locus_tag="PKI68_02835"
     CDS             complement(5784..8612)
                     /locus_tag="PKI68_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNX52661.1"
                     /translation="MKPIRIGMLGTWLLAGLIGTALGDGAAAPLPRLSPRPVAVAGCR
                     ELTVDLGGTWKFNPAAPASFAGLTAKDAAEWKPIQVPCDWAMQGFTEARGRGVAYLRT
                     FTVPAEWAGCRIKLRCDAVYSDAVVYINGKEAGRHEGGFTPFELDVTALVQPKTENVI
                     ALAVKNDSLADTLACGTKYAYHPIGGISRRISLFALPETNISSLQVTTAFDAQYRNAT
                     LTAALEIAQDGGGSFSPLEARLTLTAPDGKNVDLAPAKVAISSPTARIELPVQAPAQW
                     NPEHPNLYTLTIGLYSNGQLLESLVQKVGFRQIEVRGTQLFVNGQPIKVRGINHHEIH
                     PLTGRSVADSIDRGDVELFRNGNVNLLRTCHYPPNEALLAAADELGMFIECEAPFCWA
                     NPDKISNRVEVATALISQQTAEMAVAYRNHPSIVFWSLANESEWMPAFEASSRLLRSI
                     DPTRPQTFNWIKPKKGDEGFADILAYHYPAYTVKEKAASYTNRPIYIGEDAHLNAYNR
                     LELATDQGLRDVWGRYLRKLWDLQYSLPGVIGQSIWAGIDDVFYLPNGDIAGYGSWGP
                     LDGWRRQKPEYWNMKKAYSPVRITNVVWNGRSVELAVENRNVFMNLSEMKINWTLGKA
                     SGQVSANVPARTNGLLAITLPAVPQPGQALNLTFTDPRGFVADEFSLMMKPAEPAPFG
                     DANGAVANYKTDAATGLLSELNGLKLAGPELMILALNKEGTKERNGPEKIWTPFTSPC
                     SGWSVTGAVSPQAVSGSYEQAAGSYRYTAKPGGVLEIAYDFTVKKAINPRQVGLVFTL
                     PLSCEVLSWERTGYWDVYPEDHIARLKGSVKASEGFEATAFGPRTQPSHPWRLDKMMY
                     GNNDFCSTKHNVLRASVLDANGHGLVIDGGGQQHVRCWRTADAVYVLVADYSNAGAEG
                     FIQNLSKDDQRNLPAGSSVKGVVRIKVQ"
     gene            complement(8639..9691)
                     /locus_tag="PKI68_02840"
     CDS             complement(8639..9691)
                     /locus_tag="PKI68_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNX52662.1"
                     /translation="MTGKERVYAALNFEQTDRPPVSATFTPEIEARLRQATGIQECDL
                     GAALGNDLVKGVAGNSFEMSYYRPQKPTYVCPWGVTWRWADNAFGSFTEIHRSPLADD
                     DEESMELLKAYQIPSPYDDAQYDRLRQLIARYGQEKWIVGSCQISLFEAAWYLRGMEN
                     FMVDMMINPEYAQLLFDKVMEFVRVAGRKYIDLGVDMIWLGDDVATQKNMMMSLDTWR
                     TYLKPRYAELIREYKTANPQIKVAYHSCGNCEAIIPEMIEIGLDVLNPLQPMAISPFE
                     MKKKYGKNIALFGGLCVQQTMPNGTVEQVRAAVRKLKTECGRGGGYILSPAHHIQADT
                     DIKNVMAYYEEALRTN"
     gene            9948..11120
                     /locus_tag="PKI68_02845"
     CDS             9948..11120
                     /locus_tag="PKI68_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HNX52663.1"
                     /translation="MKSSNPPQVAVLAETTHAAGRNILKGIAQYVQEHTTWSVSYAPR
                     ALGDNATSWLKDWNGDGLIVRITAESAVDEIRQYGKPFIDMIGGRAAEYQSPQVLVAD
                     EGIGRQAAKYFLNSGFRNFAFLGMEQEVWSVCRKIGFLNTLHEHGQTLTSLELPSFYS
                     APQSPSWEHTGRQIAAWAQTLPLPCAVFCANDDLGCQLIEAARRAGLRIPDELALLGV
                     DNDVLFCELCRPPLSSIDANHFAAGYQAAAMLDRLMRGEKLKDQVLYVPPGEITVRQS
                     SSSLAITDPNLIAAARFIESQATRALSVDEVAEQAFLSRSVLQRRFKEAFGQTVHERI
                     LQERIRCAQKLLTGTALSIAEIAERAGFSHPEYLGVVFRKHLNTTPAQYRKNAARH"
     gene            complement(11169..14366)
                     /locus_tag="PKI68_02850"
     CDS             complement(11169..14366)
                     /locus_tag="PKI68_02850"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HNX52664.1"
                     /translation="MNKVTAEREPITIPTYGLGAPEKNPLFFEKRVYQGSCGKVYPVP
                     FIDKVFDTPEDQAYDSVRLENDYVRLVMLPEIGGRIFLGQDKVNQDYDFFYRQDEIKP
                     ALVGLAGPWISGGVEFNWPQHHRPGTYMPADVHIEEKEDGERIVWLSEHDPLNRLKGM
                     HGISLKPGSALVELKARLYNRTPFTQTFLWWANVAAEVHDNYQSFFPPDVHYVADHAV
                     RALSAFPIACNDYYGVDYANRPGANDLSRYKNIPVPTSYMICDTQFDFFGGYDYDAGG
                     GFIHVADRYLSPGKKQWTWGNHDFGWAWDRELTDRVGPTGRPAPYIELMAGVYTDNQP
                     DFTYLLPYETKTFSQFWWPYKKIGPVQNATKDAAVRMVRNADGTLDLGAVASRKLAGV
                     RIVLREGAAVLLDETVDLSPDQPWHNPAFPFAGDFQALEISVEGFVAYQPVDVSKLER
                     NRDKATEPPMPDAIESIDELYLTAEHLEQYRHPTRYPEIYWDEILRRDPLDARTNIAY
                     GRKKLNQGLLSEAAAYFETAIQRLTLRHPNPCTGEAHYYLGLVRRFQGFEKAAYAALY
                     KATWSYEWRAAAYYELALLDCRRGNFSQAVEHCEASLDTNRQHNKAQVLKAIALRKLG
                     QESSRGLEALLAVDPLDHWARFESGDIDGFLAKSRNDAQTVLDIAYDYADAGCTAEAV
                     RLLELHHANRVAAVAVPNPLQKSQMTHYALAWLTQDVRALENARRLGPDYFFPSRLHD
                     QLVLEWALQQPGGDSNAAYALGNYYYDKKRHEDAIAVWEKVAPFATVRRNLGIAYWNV
                     RRDGEAARQAYLQALKLDPADARIFTEYDQLREKLGDPAEARLLTLLEHPELVAERDD
                     CSVALASLYNQTGAPEKALELLLNRRFHPWEGGEGKVLKQYTTARLLLGQNALEAGDA
                     AAALQHFELAMQMQENLGEAYHLLQAKADVSYWQGKALRALGQEADAVAKFESSANEA
                     GDFQAMAVTEHSEQSYYRGLSLLELGRTEDARQLFESLKAYAEKEMEAEAKIDYFATS
                     LPLLLVFEDDLNQCKRQNAERLMELARKGLARIPA"
     gene            complement(14389..17208)
                     /locus_tag="PKI68_02855"
     CDS             complement(14389..17208)
                     /locus_tag="PKI68_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52665.1"
                     /translation="MKSMLVRVLFFILAATAVAQEQTIDLSGRWRLAMDRDNVGEPQK
                     WFGETLPGSDMITLPGSMQAQGFGDAPGMNTSWIGQIKKKEWEKPQYAPYRTAENFKM
                     PFWLQPDKYYKGVVWYQRTVEIPENWDGKFIELELERAHWQTTVWVDSSLVGSNTTLS
                     VPHRYDLSRVLKPGRHLLTIRVDNRMVINIGQNSHSMSDHTQSNWNGLIGELKLIARA
                     ATRIEELQIYPDLAGRSIKVITRLAGASGKTDGERLRFDLVFDGKAVAAETVSTRAIP
                     DGGSETIIRINDPLKVWDEFHPNLYTLITRLESAAGVDEERTVFGLREITKAGNRILL
                     NGRPVVMRGTLECCIFPLTGYPPTDVESWKRIIRICKAHGLNHIRFHSWCPPAAAFDA
                     ADELGFYYQVECSTWPNQGTSLGDGLPVDQWLYAEADAILRAYGNRPSFLLFAAGNEP
                     GGKKGRDPYLGKWVSHYRSKDSRHLVTSSSGWPELPENDYHDFCRDARIQNWGAGLTS
                     RINSRAPETVTDYSPIVAKYPNQPVITHELGQWCVYPNFKEMEKYTGVLKPRNFEVFM
                     DFLKQKKMLDQADAFLMASGKLQVLCYKEEIESNLRTRGFGGFQLLDLHDFPGQGTAL
                     VGVLDPFWDSKPYVTPAEYHRFCAPIVPLARLPKRVFKSSDTLSAQLELSQFGPADLK
                     NVTIDWSLKDKAGRAVKRGSLHKDLLPAGDLQGLGNVEVNLADLPAPAKYSLEVSVAG
                     TDAVNDWDLWVYPETVPLQSDKVTVVDALDANALGVLAQGGKVLLAVKPAQVKTSVAL
                     GFSSIFWNTAWTDNQPPHTLGILCNPQHPALAAFPTEYHSNWQWAVPIQHAATLELDS
                     LPQELKPIIQVVPDWFAPKKLALAFEAKVGGGSLLVCSVDLQTDLASRLEIRQLKSSL
                     LAYMESAAFAPAVELSPEAINSLLK"
     gene            17381..18334
                     /locus_tag="PKI68_02860"
     CDS             17381..18334
                     /locus_tag="PKI68_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX52666.1"
                     /translation="MAKTIVLDGLSYSVIHTLAAMTPPPARIKEGFPKQRLIVLPANV
                     IHRAQQLPLVSGLYVTHIGSYPKAPHHYVERKTGAEEIILICCTGGRGWIKLGREQVE
                     VGPGHLILIPAGTPHRYGAARTDPWSIFWIHFTGPQAAALLQTLDVSREKPLLFVPDL
                     ALMQQAFEDLYATLNYHYSDAGLLAMTGELIRLVSCIKLHQGHPERHRQAVEDRVLET
                     IRFMQDHLDMPLTLEALAARAGQSASHYHKLFKKRTGQSPLAYFIQLKIIKACALLSQ
                     TGQTVQEIARGLGYDDPYYFSRLFKKVQGCSPANYRRSVEN"
BASE COUNT         3680 a         5597 c         5106 g         3926 t
ORIGIN      
        1 gccggtatgc agccgaaaca ttcgcgcaaa tactgccgtc caacagatga tgcagcgcaa
       61 ctgctgaaga cggcggtgtc ggaaatgaac ttcagtgccc gcgcctatga ccggattctg
      121 aaagtcgcgc gtaccatcgc cgaccttgcg aacaaggagc agatcggcac agaacacatc
      181 gccgaagcga ttcagtaccg gacgctcgac cggatgggat aaatggaaaa tgcgggcgcc
      241 cgtactgggt ctgcagtccc gtaaggcacc cgcctttcca aaaattacca gagagcgata
      301 ataagtatga cgacgatcag gctggtcaca actaacaaat aactgagagc cgcccgcacc
      361 gcctgccgga tacgcaaaac agatggatat tcctgcatca ccgctcctct tttaactgcc
      421 ggtcctgaac gtagtcacag atcgctttgt cgagcacacg gcggatcggc tgcttatgct
      481 gccggcagta ttcctgcagc cgttgtttga cttcgctcga aatccgaaca ctttcggaag
      541 tctctttcga actgctggcc gcttcgctca tggcgacaag ttgccacaat tgtggcaaag
      601 atcaaaataa aaattgtgct gattttatgt tttggattcg gtgggatttt atctacagtg
      661 cccttatgag cgataccgaa tcagtccgga tcagcaaaga gttgaagcag cagttaaagc
      721 agatggctga aaccatgcgc ccgcgcacgg ccattcagta tctgattgaa gatgcgattg
      781 aacagtacct cgaccgtgcc gccgaaggaa acgggccgct ttatcaggtt aagccggaat
      841 cgaagacgaa gaaaaagccg taaccctccc ttacgcagga ttcatgcgat tgagttttca
      901 tgccagaaac cctttataaa actgccggta tccttttggc tctgctggcc atgagtctgt
      961 tcaatctggc tccggtatta cagaagtctg cactggaccg cctgccgccg cttttatttt
     1021 ctaacctcag gaaatccatc atcagcctac tgaaagaccg gcgctggatg attggtttta
     1081 taatcggctg tctgggcctg ctgccctatg tggcggcaat gagtctggtc ggggtggcgg
     1141 tggtacagcc gctgtacgga ttcggcttca tcgtgctgct ggttgtttcc cggttcatgc
     1201 tgcatgaaaa actgcatccg gcggcgaaac tggcgatggg cctgctgatt ctgatgccgg
     1261 tgctcattgc gctcggcgat gtgtccggcg ttcaatacga tatcacgcgg cgcagcacac
     1321 agatcagcct cggcgttttc gtgacgatca ctctgctgac cagcgtcgtt ctgatctttt
     1381 ttacgaaaaa gcatcccctt tactgggcgg tgatttccgg cgccctcttc ggtgccggtg
     1441 cggttttttc acaactggct atcaccttcc tgactgcgga tgaactgcgt gacctaaccg
     1501 gtttcaccac aatcatctgt aataagaacc tgctgttctg tctggctgcc gtactggcgg
     1561 ccacgacggt caatgtgctg gcgggctatt ttatgcaggt tggcttgcag cagaaaatgg
     1621 catcgcagtt tatgcccata acacagaccg taaataacgc cgtggcgatt acgggcggcg
     1681 ttaccctttt cggtcagtgc gtaacgcact ggtcttttta cattgccggc attgtaatgg
     1741 gacttgccgg gctttttctg ctggcgaggt tcaagcatcc gtccgaactg gattttaaat
     1801 ccggggaata gcgtcgtcgc tgcccggcgt cagcttccat cccagccggc ggagtttttc
     1861 cttcgccttc ccgccggtac tttcacgggg aattatcacc gcttcagtat gaatctcatc
     1921 aaccagtttt tgaataatgc gcgatgcggc atccggatgt gacagtccga gcgcgcggcg
     1981 gcgcatcttg gccatccgct ccggatcttt cagcagcagg tttaccttgt aggccatcgt
     2041 ggcgagctgg ttgcacttca ctgccgcacc gttttccagc aggaagtcgc tgttgcgctc
     2101 ctcctgcccg ggaatcggct gatagatgac catcggcagc ccgcaactca tcgcttcagc
     2161 ggtggtcaga ccgccgggct tgccgatgta gagatcggcg gatttcatcc attcatccat
     2221 ttcggtggtg tatcccagca ctttgaagtc gaggtgcgac gcctcatccg gacgccggct
     2281 gagctgatcc agcaccatct gccgcagctt ttcatttttt ccgcagatca ccacaatctg
     2341 caccggaaca gccagctcgc tgagtaccgc gacaatctgt tcggcggaac tgacgcccag
     2401 cgcgccggcg gaaaccagca gcatgggaat gtcgttgcgc agcccctgtt tcgcgcgcag
     2461 cgctgcccgg ttcttcggct cgcagaatac cggatcgatc ggtatgccgg aaacggtgat
     2521 gctttccggc gggaacccga tgttttcgag ctgtacccgc gattcttcgg tggccacaaa
     2581 gtagtgatga atcagccgcg acagccacat ggcatgcgca taataatcgg tgacggtaat
     2641 cgaaagctgc gtgtctatct ctccgtgctt gatcaggtgc gaaagaattt cggacggcag
     2701 aaaatgggtg cagatggtaa tgtccggttt gaatttttta atctgccgga cgagcggacc
     2761 ggtattcatc cggtcaagca tgagacgcat tttttccgtt ttccacggtt catcctgaga
     2821 gtcataaaac cagccgagaa gcgtgggagc gcttttgacc agatcgatat aaaactgtcc
     2881 gtagaaccgg cggaacattt tgttggtata gcgcagagca tcgatatgca gcacttcgcc
     2941 ggaggcagga tgagccgccg cggctttggc gagcgcttca cccgcgcgga tgtgaccggt
     3001 tcccgcgctg acggaaagaa tcagcacgcg atgcccgggc ctctgtggcc atgacttcat
     3061 atgatctccc gataaaccag ctcgtgaacc agccggtcga cttcggcgtg cgcggtttca
     3121 atgaacgcct cgaagtcttc ggctcttctt ccggtcggat cgggcacgtc gagcgtttcc
     3181 gccgcgtcat cgccctgcgc gccgtattcc attagccgga aaaccttctc ttttgattcc
     3241 ggatattttt cgagaatgaa atctttgtgt tcctgctcca tcaccagaat cagatcggcc
     3301 tgcttcatca gcttgccgga aagcggctcc gccatatgct gacgcaaact gaacccctgt
     3361 ttgcgggcaa tcagcgcgac cacatcattg atccgtccgc cgcccgccgc cttcgtgccc
     3421 gccgaagtga ttttaattcg gcggagcgcg tccggacggt attgcgaaag gtagtgccgg
     3481 aggcagccgg cgaaataggc gctgcgcgtg cggttagccg tgcaaacgat gatcagatgg
     3541 aatttcttcc tgaagaaaaa catgcgctga gtaataaatt aaaacagccg ttttaacaag
     3601 ctgaacatcc gtgtgttggc ggcttcaagc gcaggaaaaa acggccgcat ttgttccagc
     3661 gtctggataa acggtccgtc cgtgatttca ccggagcagt tttgataaag cgacatcagg
     3721 ctttcttccg ttgtttccag atggaactga agcgccagca cgttgtcttt gaaactgaac
     3781 gcctgatttt tacaggcttc gctcgacgcc agatgaaccg cgccgtccgg aatgccgaac
     3841 gtgtcaccgt gccagtgaaa cgccgtgaac tccgccggaa ataaagcgcc gtcaccggcc
     3901 aacggaaaga aaccgatttc gcgctgatcg tttttccaga ccctggcacc cagtacttcg
     3961 gcgatgagct gcgcgccgag acagatgccg agcaccggct tatcctgagc aacgatttgt
     4021 ctgatgaaag ctttctcatc cgccagccat ggataaaccg cctcatcata aacgcccatc
     4081 ggtccgccca tcacaatcag tccgtcgatt ttttcagccg ccggaaaacc atctccggcc
     4141 caaagccgcg tacaggtgac agcgtgacgg cgttcggcca gccacggcgc aatgcatccg
     4201 aggccttcaa acggaacatg ctgaagccag tgaattttca tgctttggag cgaatcataa
     4261 tcagcagcat tttaaaagga gcgtccgctg cctgcacgtc atgcggaatg ttggcgggca
     4321 tgatgatcag ttcgccggct ttgaccgttt gcggttcgcc cgcgatgaca aaggttcctt
     4381 cgccctcgac cacttcgacc acggcatcaa acggcgaagt gtgttcactt aacccctgcc
     4441 ctttatcgaa cgaaaagagc gtaatgttgc cggtttcttt tttgagcagc gtacggctga
     4501 cgaccgcgcc gtcggcatag tttaccagcg ttttcannnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnccgc tccgtcgcgc ggcttcttga accatcatcg tcagtcctcc
     4681 gcagcaggga acttccatgc gaacaatgat aactttcgga atgccgttcg aggaaagaat
     4741 ggccgccagt ttttcaacgt agccttccgt gcggtcaagc ttcggacagg caatggcaat
     4801 cgcccggccg cgaacgaacc gccagttgac atccggcgaa gccgacggcg aacaggtgct
     4861 gagcaccacc agttcgcggt ttttaaaaaa cggcgcttcg accggcacca gatgcagctg
     4921 cgtcggccac tggttaagtt ccgacgggat aacctgcccg gaaacgccct gcccttcgat
     4981 cttcgacttg gcaacttttg ccggctccgc aaaacggagc ttcattccgg ggcatccgcc
     5041 ggaaggctgt gcggttttct gtaagtgttt ctgtacggcg gtttcgctga attccggcgc
     5101 atcccgctca ataatcttca gcgctcccgt cgggcattcc ccgatacagg cgccaagccc
     5161 gtcacagaaa acatcgttca ccagttttgc tttgccgttc acaacagcaa gcgctccttc
     5221 ggcgcagccg gtaacacagg ctccgcagcc gttgcacttc tcctcatcga tctcaataat
     5281 tttgcgcttc atgccgtttc tccatttact gagacagatt ctaccacacc ttaaaacggc
     5341 ggcattgatc cgcgtcaagt tctcaaaaac ggcccgtttt gtagggaaca acccgacaag
     5401 aaaaatcttt tgtaggctta ctggcaatgt atagtccgcc ctcagagttt cggagaggaa
     5461 gacacttagg ggtttgttgc cgtcttctct gattctttcg acaacgaaag agctcgacct
     5521 ccgcaaccgg ttttccggtt gcggaggttt tttattccgg aacagcccga cgctctggga
     5581 aatttcccgg aatgtctttg ccgggaatgc agactgcagg tctgagactt tcgacggcag
     5641 agcgtattct aaaattctgc cgccgttctt ttcttcgtcc gcggctgccg cttttccggc
     5701 cggcagctcc aaacaaaaaa ccgcgcacgg atgcgcggtt ttttgcgttt agaagaccgc
     5761 cgcgcggctg cgcggcagtt gagttactgt actttgatgc gaacaacgcc cttcacagag
     5821 gacccggccg gaagattccg ttggtcgtct ttcgacaggt tctggataaa gccttcggcg
     5881 ccggcgttcg aataatcggc caccagcaca tagactgcgt cggccgtgcg ccagcagcgg
     5941 acgtgctgct gaccgccgcc gtcgatgacc aggccgtgtc cgttggcatc cagtacactg
     6001 gcccgcagga cgttgtgctt ggtcgagcag aagtcgttat tgccgtacat catcttgtcc
     6061 agacgccagg gatgcgacgg ctgggtgcgc gggccgaaag ccgtcgcctc aaacccttcc
     6121 gaagctttga cgctgccttt cagccgggcg atgtgatctt ccggatagac atcccagtag
     6181 ccggtgcgct cccagctcag gacttcacac gacagcggca gcgtaaagac cagtccgacc
     6241 tgccggggat taatcgcttt cttcacggtg aagtcatagg cgatttcgag cacgccgccg
     6301 ggcttcgccg tgtagcggta gctgccggct gcctgttcat agcttccgct gaccgcctgc
     6361 ggcgaaacgg caccggttac cgaccagccg ctgcacgggc tggtaaaggg agtccagatt
     6421 ttttccggcc cgttgcgttc tttcgtacct tccttattca gcgccagaat catcagctcc
     6481 ggtccggcca gtttcagccc gttgagttcg ctcagcagtc cggttgcggc atctgtttta
     6541 taattcgcca ccgcaccgtt cgcgtcgcca aacggcgccg gttcagcggg cttcatcatc
     6601 agactgaatt cgtccgccac aaaaccgcgc ggatcggtaa aggtcagatt cagcgcctgg
     6661 ccgggctgcg gaactgccgg cagcgtgatg gccagcaaac cgttcgtccg cgccggaaca
     6721 ttggcgctca cctggccgga ggccttgccc agagtccagt tgattttcat ctcgctcagg
     6781 ttcatgaaga cattccggtt ttcgaccgcg agctcaacgc tccgtccgtt ccagaccacg
     6841 ttggtgatcc gcaccggaga ataggctttc ttcatattcc agtattccgg cttctgacgg
     6901 cgccagccgt cgagcggtcc ccaggaaccg tatccggcga tatccccgtt cggcaggtag
     6961 aagacgtcat cgataccggc ccagatcgac tgtccgatca ctcccggcag gctgtactgc
     7021 agatcccaca gtttgcgcag gtagcggccc cagacatcgc gcaggccttg atcggtagcc
     7081 agctcgagcc ggttataggc attcagatgc gcgtcttcgc cgatgtagat cggccggttt
     7141 gtgtagctgg ccgctttttc tttaacggtg taggccggat agtgataggc cagaatgtcc
     7201 gcgaagcctt catccccttt tttcggtttg atccaattga acgtctgcgg gcgtgttgga
     7261 tcgatgctcc gcagcagccg cgaggacgcc tcgaaggccg gcatccattc gctttcattc
     7321 gccagcgacc agaaaacaat cgaagggtga ttgcgataag cgaccgccat ttccgccgtc
     7381 tgctggctga tcagcgccgt cgcaacttcg acccgatttg aaatcttgtc cgggttggcc
     7441 cagcagaacg gtgcttcgca ttcaatgaac atccccagct catctgccgc cgccagcaac
     7501 gcttcgttcg gcggataatg gcaggtgcgc agcaggttga cgttgccgtt gcggaagagc
     7561 tccacatcgc cccggtcaat cgaatccgcc acggagcgtc cggtcagcgg atgtatttcg
     7621 tggtgattaa tgccgcggac tttgatcggc tggccgttca caaacagctg cgtgccgcgc
     7681 acttcaatct ggcggaagcc gaccttctgc accagcgact ccagcagctg tccgttggaa
     7741 tacagtccga tcgtcaaggt atagaggttg ggatgttccg gattccattg tgccggagcc
     7801 tgaaccggca gttcgatccg cgccgtcgga gaactgatcg cgaccttcgc cggggcaagg
     7861 tcgacgttct tgccgtccgg agccgtcagc gtcagacgcg cttccagcgg cgaaaagctc
     7921 ccgccgccgt cctgtgcgat ttccagcgcc gccgtcagcg ttgcgttgcg gtattgcgca
     7981 tcaaaggcag tcgtcacctg aagcgaagag atattcgttt cgggcagcgc aaacagactg
     8041 atccgccgcg aaatgccgcc gatcgggtga taggcatatt tggtgccgca ggccagcgta
     8101 tcggccagcg aatcattctt cacggccagc gcgatgacgt tttcggtctt gggctgaacc
     8161 agcgccgtga catccagctc gaacggcgta aagccgcctt cgtgccggcc ggcttctttc
     8221 ccgttgatat agaccacggc atcgctataa accgcgtcgc agcgcagttt gatccggcat
     8281 ccggcccact cggccggaac cgtaaacgtc cgcagatagg cgacgccacg gcctctcgct
     8341 tcggtgaatc cctgcatggc ccaatcgcag gggacctgaa tgggtttcca ctcggcggcg
     8401 tctttggccg tcagtccggc aaacgaagcc ggcgcggccg ggttgaactt ccatgtgccg
     8461 cccagatcaa ccgtcagttc gcggcagcct gccacggcaa ccggacgcgg actcagccgc
     8521 ggcagcggtg cggcagctcc gtcacccagc gccgttccga tcagtccagc caataaccat
     8581 gtccccagca ttcctattcg tatcggtttc atttccatca ttcctttcgt tggctcactt
     8641 aattcgtacg cagtgcttct tcgtaatagg ccattacatt cttaatatcg gtatccgcct
     8701 ggatgtggtg cgccggcgaa agaatatagc cgccgccccg cccgcactcg gtcttcagct
     8761 tccgcacggc cgcccgcacc tgctcgacgg tgccgttcgg catcgtctgc tgtacacaca
     8821 gtccgccgaa cagggcaata ttcttgccgt atttcttttt catttcaaac gggctgatgg
     8881 ccatcggctg cagcggattg agcacatcga gtccgatttc gatcatttcc ggaatgatgg
     8941 cctcgcagtt gccgcagctg tgataggcca ccttaatctg cggattggcg gtcttatatt
     9001 cgcggatcag ctcggcatag cgcggtttga gatatgtccg ccaggtgtcg agtgacatca
     9061 tcatattctt ctgcgtcgcc acgtcatcgc ccagccagat catatccacg ccgaggtcga
     9121 tgtatttgcg ccccgcaacg cgcacaaatt ccatcacctt atcgaacagt aactgcgcgt
     9181 actccggatt gatcatcata tcgaccatga aattttccat gccgcgcaga taccaggccg
     9241 cttcgaacag actgatctgg caggacccga cgatccactt ttcctgtcca tagcgcgcaa
     9301 tcagctgccg cagccggtca tactgcgcat catcatacgg ggaaggaatc tgataggcct
     9361 tcagcagctc catggactct tcatcatcgt cggccagcgg agagcggtgg atctccgtaa
     9421 agctgccgaa cgcattgtcg gcccagcgcc acgtcacgcc ccacgggcag acatacgtcg
     9481 gcttttgcgg ccggtaataa ctcatctcaa agctgtttcc cgcaacgccc ttcaccagat
     9541 cattgcccag tgccgcgccc agatcacact cctgaattcc ggtggcctgc cgcaggcggg
     9601 cctcgatttc cggcgtaaac gtcgccgaga ccggcggacg gtccgtctgt tcaaaattga
     9661 gtgccgcgta aacccgttcc ttacctgtca ttgaattttc tcctcgttca ttacctgtcg
     9721 ctaaatcact gcgctttgat gagaacttcg catccgtttc atattcaccg gccccttcgt
     9781 ttctacgcag caggcccttc cataagaatg aacagcatcg cccagcggta ttatcggcca
     9841 ttattaagcc atcgccgaga aactgaacag gtgttttaaa accatacttt ggagaaatcg
     9901 ggtcaaaacg gctggcgcaa tcccgccaga agccgtatgc tcgcgccatg aaatcatcca
     9961 atcctccgca agtcgccgtg ctggccgaga caacccacgc cgccggccgc aacatcctca
    10021 aaggcattgc gcagtatgtg caggagcaca cgacgtggtc ggtctcctac gcaccgcgcg
    10081 cactgggcga taatgcaacc tcgtggctga aagactggaa cggcgacggc ttaatcgtgc
    10141 gcatcactgc cgaaagcgct gtggacgaaa tccgccaata cggcaaaccc ttcatcgaca
    10201 tgatcggcgg gcgggccgcg gaataccaat ctccgcaagt gctcgtcgcc gacgaaggca
    10261 tcggacggca ggccgccaaa tatttcctga acagcggatt ccggaatttt gcgtttttag
    10321 gaatggagca ggaagtctgg tctgtctgcc ggaaaatcgg cttcctgaac accctgcacg
    10381 aacacgggca gaccctgacc tcgctcgaac tgccgtcgtt ttacagcgct ccgcaatcgc
    10441 cctcctggga acataccggc cggcaaatcg ccgcctgggc ccaaacgctg cccctgccct
    10501 gcgcggtctt ttgcgcgaat gatgatctcg gctgccagct gatcgaagcc gcccgccgcg
    10561 ccggactccg catccccgac gagctggccc tgctgggggt tgataatgat gtcctcttct
    10621 gcgagctctg ccggccgccg ctctccagca tcgacgccaa ccacttcgcc gccggttatc
    10681 aggcggccgc catgctcgac cgcctgatgc gcggcgaaaa actgaaagac caggttctct
    10741 atgtcccgcc cggcgaaatc accgtgcggc aatcctccag cagtctggcg atcaccgacc
    10801 ccaatctgat tgccgccgca cgattcatcg aaagccaggc cacccgcgcg ctcagcgtcg
    10861 atgaggttgc cgagcaggcc tttctctccc gcagcgttct ccagcggcgc ttcaaagagg
    10921 ccttcgggca aaccgtgcat gaacggatcc tgcaggagcg gatccgctgc gcacagaaac
    10981 tgctgaccgg cacggcgctc tccattgcag aaatcgccga gcgggcgggc ttcagccacc
    11041 cggaatatct cggcgtcgtc tttcgaaaac acctgaacac cacgccggcg caataccgca
    11101 aaaatgccgc gcgccactga aggagacgcg cggccggacc gaagtgccac ggatagaagc
    11161 ggcggcggtt acgccgggat gcgcgccagc cctttccggg cgagctccat caggcgttcc
    11221 gcgttctgcc gcttgcactg attcagatcg tcctcaaaga ccagcagcag cgggagcgag
    11281 gtggcgaaat aatcaatctt cgcctcggcc tccatttcct tttccgcata tgccttcaaa
    11341 ctttcaaaca gctggcgggc atcctccgtg cgccccagct ccagcagcga caggccgcga
    11401 tagtaggact gttcggaatg ctcggtgacg gccatcgcct gaaagtcccc ggcctcgttg
    11461 gcactggact cgaatttcgc caccgcgtcc gcctcctgac cgagggcgcg cagcgctttg
    11521 ccctgccaat agctcacgtc ggctttggcc tgcagcaggt gataggcctc gcccagattt
    11581 tcctgcatct gcatggccag ttcaaaatgc tgcagcgcgg cggcggcatc gccggcctcc
    11641 agcgcgttct gccccagcag caggcgggcg gtggtgtact gcttgagcac cttgccctcg
    11701 ccgccttccc acgggtggaa gcggcggttc agcagcagct ccagcgcttt ttcgggcgca
    11761 ccggtctgat tgtagagcga cgcaagcgcc acggagcagt cgtcacgctc cgccaccagc
    11821 tccggatgct ccagcagcgt gagcagacgc gcttcggccg ggtcgcccag tttttcacgc
    11881 agctgatcat attccgtgaa aatgcgcgca tcggcgggat cgagcttcaa ggcctgaaga
    11941 tacgcttggc gggcggcttc gccgtcgcgg cggacattcc agtaggcgat cccgagattg
    12001 cgccggacgg tcgcgaacgg tgcaactttt tcccagactg caatggcatc ttcgtgccgt
    12061 tttttgtcgt agtaataatt gcccagcgca taggcggcgt ttgagtcgcc gcccggctgc
    12121 tgcagcgccc actcgagcac gagctggtcg tgcagccgcg acggaaagaa ataatccgga
    12181 cccagccggc gcgcattttc caatgcgcgc acatcctgcg tcagccaggc cagagcataa
    12241 tgcgtcatct gcgatttttg cagcgggttg ggaacggcga cggccgccac gcggttcgcg
    12301 tggtgcagct ccagcagccg gacggcttcc gcggtgcagc cggcgtcggc atagtcgtat
    12361 gcgatatcga gtacggtctg cgcatcgtta cggctcttgg ccaggaaacc gtcgatatcg
    12421 ccggactcaa agcgcgccca gtgatcgagc ggatcgactg cgagcagggc ttccaacccg
    12481 cgggaactct cctgtccgag cttccgcaag gcgatggctt tgaggacctg cgctttgttg
    12541 tgctgccggt tggtgtcgag cgaggcctcg caatgctcca cggcctggga aaaatttccc
    12601 ctgcggcagt cgagcagcgc cagttcgtaa taggcggcgg cgcgccactc ataactccag
    12661 gttgccttgt aaagtgcggc gtaggccgct ttttcaaagc cttggaaacg gcggacgagt
    12721 ccgaggtaat agtgggcttc gccggtacac gggttcgggt ggcggagggt cagccgctgg
    12781 atggccgtct cgaaataggc cgcggcttcg ctgagcagcc cctgattcag ttttttgcgg
    12841 ccgtaggcga tgttggtgcg cgcatccagc ggatcgcggc gcagaatctc gtcccagtaa
    12901 atttccgggt agcgggtcgg atggcggtac tgctccagat gctcggcggt cagatagagt
    12961 tcgtcgatcg actcgatcgc atccggcatc ggcggctcgg tcgccttgtc gcggttccgt
    13021 tccagcttgg aaacatcgac cggctgatag gcgacaaacc cttccacaga aatttccagg
    13081 gcctggaaat cgccggcaaa cgggaacgcc gggttgtgcc acggctggtc gggcgaaaga
    13141 tcgacggttt cgtcgagcag caccgcagcg ccttcgcgca atacgattcg cacgccggcg
    13201 agtttgcgcg acgccaccgc cccgagatcg agtgtcccgt ccgcgttgcg caccatgcgc
    13261 acggcggcgt ccttcgtggc gttctgcacc gggccgattt ttttgtaggg ccaccagaac
    13321 tgcgagaagg ttttggtttc gtagggcagc agataggtga agtccggctg attgtcggtg
    13381 tagacgccgg ccatcagctc aatgtagggc gcgggccttc cggtgggccc gacgcggtcg
    13441 gtgagctcac ggtcccaggc ccagccgaag tcgtggttgc cccacgtcca ctgctttttg
    13501 ccgggggaca ggtaccggtc ggccacgtga atgaatccgc cgccggcgtc gtaatcgtag
    13561 ccgccgaaaa agtcgaactg cgtgtcgcag atcatatagc tggtcggcac cggaatgttt
    13621 ttgtagcggc tcaggtcgtt ggcgccggga cggttggcgt aatccacacc gtaataatcg
    13681 ttgcaggcga tcggaaaggc gctcagggcg cgcacggcgt ggtcggcgac gtagtggacg
    13741 tccggcggaa agaagctctg atagttgtcg tgcacttccg cggcgacgtt cgcccaccac
    13801 aggaaggtct gggtgaacgg cgtgcggttg tagaggcgcg ccttgagctc cacgagcgcg
    13861 ctgcccggct tcaggctgat gccgtgcatg cctttcaggc ggttgagcgg gtcgtgctcg
    13921 gagagccaga cgatgcgctc gccgtcctct ttttcctcga tgtgcacgtc cgcgggcata
    13981 taggtgccgg ggcggtgatg ttgcggccag ttgaactcca cgccgccgga aatccacgga
    14041 ccggccaggc cgaccagtgc cggcttgatc tcatcctgcc ggtaaaagaa atcgtagtcc
    14101 tggttgacct tgtcctgccc cagaaaaatc cgcccgccga tttccggcag catcaccagc
    14161 cggacatagt cgttttccag ccggacggag tcataagcct ggtcctccgg ggtgtcgaac
    14221 accttgtcga tgaacggcac cggatagacc ttgccgcacg agccttgata gacgcgcttt
    14281 tcaaaaaaga gcgggttttt ctccggcgcg cccagcccgt aggttggaat ggtgatcggt
    14341 tcgcgttcgg ctgtaacttt gttcataacg tccgtccttc ggtttgtatc acttcaacag
    14401 cgagttgatg gcttcgggtg aaagctccac cgccggggca aatgcggcgc tttccatata
    14461 ggcaaggagt gagcttttga gctggcggat ttcgagccgg cttgccagat cggtctgcag
    14521 gtcgacgctg cagacgagca gcgacccgcc gcccaccttc gcctcaaagg cgagcgccag
    14581 ttttttcggc gcgaaccagt ccggcaccac ctgaatgatc ggcttcagct cctgcggcag
    14641 actgtccagc tccagggtcg ccgcatgctg gatcggcacg gcccactgcc agttgctgtg
    14701 atattcggtg gggaacgccg ccagagccgg gtgttgcgga ttgcacagaa ttccgagcgt
    14761 gtgcggcggc tggttgtccg tccaggccgt gttccaaaaa atcgaggaga acccgagggc
    14821 caccgatgtt ttaacctgtg cgggcttcac ggccagcagc actttgcctc cctgcgccag
    14881 cacaccgagc gcgttggcat cgagcgcatc gaccaccgtt accttgtccg actggagcgg
    14941 aaccgtttcg ggatagaccc agagatccca gtcattgacg gcatcggtgc cggccaccga
    15001 gacttccagc gaatatttag ccggcgcagg cagatcggcg agattgacct ccacattgcc
    15061 cagcccctgc aggtcgccgg ccggcagcag atctttatgc agcgagcccc gtttcacggc
    15121 ccgcccggct ttatccttca gcgaccagtc gatggtcacg tttttgagat ccgccggacc
    15181 gaattgactg agctccagct gagccgacag ggtgtcggac gatttgaaca cccgtttcgg
    15241 caggcgggcg agcggaacga tcggcgcgca gaaccggtgg tattcagccg gcgtcacata
    15301 cggcttggaa tcccagaacg gatcgagcac accgaccagc gcggtgccct gcccggggaa
    15361 gtcgtgcagg tcgagcagct ggaatccgcc aaatccccgc gtgcgcaggt tggactcaat
    15421 ttcttcctta tagcagagga cctgcagctt gccggaggcc atcaggaacg catcggcctg
    15481 atcgagcatc tttttctgct tcagaaaatc catgaaaact tcgaagttcc ggggcttcaa
    15541 caccccggtg tatttttcca tctctttgaa attcggatac acacaccact gtccgagctc
    15601 atgagtaatc accggctgat tcgggtattt ggccacgatc ggactatagt ccgtgacggt
    15661 ctccggagcg cgactgttaa tgcgactggt cagcccggcc ccccagttct gaatgcgcgc
    15721 gtcgcggcag aaatcatggt agtcgttttc gggcagttcc ggccatccgg aggagctggt
    15781 taccaggtgc cgggaatctt tgctccggta atgcgaaacc cactttccga gatatggatc
    15841 ccgccccttt ttaccgcccg gctcattgcc ggcggcaaac agcagaaagg agggacggtt
    15901 gccataggcg cggagaatcg catcggcttc cgcatacagc cactggtcaa ccggcagccc
    15961 gtcacccagc gacgttccct gattcggcca ggtggaacat tcaacctgat aatagaagcc
    16021 gagctcatcg gcggcatcaa aggccgctgc gggcggacac caggaatgaa agcggatatg
    16081 gttgagaccg tgcgccttgc agatgcggat gatgcgcttc cacgactcaa catcggtcgg
    16141 cggatagccg gtcagcggga aaatgcagca ctccagcgta ccgcgcatca cgacaggccg
    16201 gccgttgagg agtatacggt ttccggcttt cgtaatctca cgcagaccaa acaccgtgcg
    16261 ctcttcatca accccggctg cgctttcgag acgggtgatt aacgtgtaga gattcggatg
    16321 aaactcatcc cagactttaa gcggatcgtt gatccggatg atcgtctcgc tgcccccgtc
    16381 cgggatggcc cgggtggaca cggtttccgc cgcgaccgct ttcccgtcaa aaaccagatc
    16441 aaatctcagc cgctctccgt ccgtttttcc ggatgcgccg gccagacggg ttatcacctt
    16501 gatggagcgt ccggcaaggt ccggataaat ctgcagctcc tcgatccgcg tggcggcgcg
    16561 ggcgatcaat ttcagctcgc cgatgaggcc gttccagttg gactgcgtat ggtccgacat
    16621 gctgtgtgaa ttctgtccga tattgatgac catccggtta tcgacgcgaa tcgtcagcag
    16681 atgccggccc ggcttcagca cacggctgag gtcataacga tgcggaaccg aaagcgtagt
    16741 attcgaaccg accagtgatg aatccaccca gacggtggtt tgccaatgcg cgcgctcgag
    16801 ttcgagttca atgaatttac cgtcccagtt ttcgggaatc tcaacggttc tctgatacca
    16861 caccacgcct ttgtaatatt tgtccggctg cagccagaac ggcatcttaa aattttcagc
    16921 cgtgcggtac ggcgcatact ggggtttctc ccactctttc tttttaatct ggccgatcca
    16981 tgaggtattc atgccggggg catccccgaa cccctgcgcc tgcatggagc cgggcagcgt
    17041 aatcatgtcg ctgccgggca gcgtctcgcc aaaccacttc tgcggttcac cgacattgtc
    17101 gcggtccatc gcaagccgcc agcggccgga caaatcaatc gtctgctcct gcgcaacagc
    17161 ggttgcagca aggataaaaa ataaaacccg cactagcatg ctcttcatat ccggctcctt
    17221 tgtactaccc aaacattcgc gcaaaaaatg cccactcatc gaatcaccgc accaccagcc
    17281 ggaccctccc aagctgaagt cccttgtcct gcacagtcct ctgaggctca ccagtttcgc
    17341 attcgactcg ccatgctaaa acgactgcat cataaagaaa atggccaaaa ccattgtttt
    17401 ggatggacta tcttattctg taattcatac cctcgccgcc atgacgcctc ctcctgcacg
    17461 aataaaagaa ggctttccga aacagcggct gatcgtgctg cctgccaacg tcatccatcg
    17521 cgcacaacag ctcccgctgg tcagcggact gtatgtcacc cacatcggca gctatcccaa
    17581 ggcgccgcat cattatgtgg aacgcaaaac cggagccgag gaaatcatcc tgatctgctg
    17641 caccggagga cggggatgga ttaaactggg ccgcgagcag gtcgaggtcg gccccggcca
    17701 cctgatcctg atcccggccg gaaccccgca ccgctacggc gccgcccgaa ccgatccgtg
    17761 gtcgatcttc tggattcatt ttaccggccc gcaggccgcc gccctgctcc aaaccctcga
    17821 tgtctcgcgc gaaaagcccc tgctgtttgt tcccgacctc gcgctgatgc aacaggcctt
    17881 cgaagatttg tacgccaccc tcaactacca ctattccgac gccggcctgc tcgccatgac
    17941 cggcgaactc atccgcctgg tctcctgcat caaactgcat caggggcacc ccgagcgcca
    18001 tcggcaggcg gtcgaagatc gcgtactcga aaccatccgc ttcatgcagg accatctgga
    18061 catgccgctg acactcgaag cgctcgccgc acgcgccgga caatcggcct cccactacca
    18121 caaactgttc aaaaagcgca ccggccaatc ccccctggcg tacttcatcc agctcaagat
    18181 catcaaagcc tgcgcgctgc tcagccagac cggccagacg gttcaggaaa ttgcacgcgg
    18241 actcggctac gacgacccct actacttcag ccgcctcttc aaaaaagtac agggttgctc
    18301 gccggccaac taccgccgct ccgtcgagaa ttaaaccgca gacggcgcag caacggcaat
    18361 ttgacggttc ggcgaagccg ctgactcagc ggaacggggg agtattttg
//
LOCUS       DAOPZO010000023        18375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_3178_length_18375_cov_7.233133, whole genome shotgun sequence.
ACCESSION   DAOPZO010000023 DAOPZO010000000
VERSION     DAOPZO010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 18375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18375
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3178_length_18375_cov_7.233133"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /gene="nfo"
                     /locus_tag="PKI68_02865"
     CDS             complement(<1..803)
                     /gene="nfo"
                     /locus_tag="PKI68_02865"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:NF002199.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HNX52667.1"
                     /translation="MKTIGAHVSASGGVENAPLNAKAIGANAFALFTKNQRQWTSAPL
                     SGESIRAFNQNCAGSFAAAQILPHDSYLINLGSPEPEALEKSRAAFLDEVQRCEQIGL
                     DRLNFHPGSHLGKCSESECIALIAESINLAHSKTKSVIAVIENTAGQGSNMGYRFEQL
                     AEIIEQIHDKTRVGVCIDTCHAFTAGYDLRTEKACAETFEAFDRIVGFKYLKGMHLND
                     SKKELGTRVDRHECLGKGFIGLDCFRFLMADKRFDNMPLILETPDPAIWA"
     gene            1213..1611
                     /locus_tag="PKI68_02870"
     CDS             1213..1611
                     /locus_tag="PKI68_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52668.1"
                     /translation="MKKWTAGVLVIGLSTALLSGCASVQTSKNLNGQKIDAAGTPVAH
                     IHAKNSGYYLLGIPLIAGQTEAGSVGKIGLGKDTVNVSSMVDVVTRESKALGGTKTVD
                     LSSRVDSMPMLPIPFVFTIESVQVSGNSVK"
     gene            1738..1998
                     /locus_tag="PKI68_02875"
     CDS             1738..1998
                     /locus_tag="PKI68_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52669.1"
                     /translation="MDKHIIVGVHITERTKHAAEIQKVFTEFGCQIRTRLGLHEAGKG
                     SCSPNGMILLDMVDDDAKVGAFKKKLLAIDGVEVQSMVFDHA"
     gene            1991..3508
                     /locus_tag="PKI68_02880"
     CDS             1991..3508
                     /locus_tag="PKI68_02880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNX52670.1"
                     /translation="MRKDGKHVERWLIPLLVLAGAALRLIQLPFSCIWLDEAFSIRLA
                     QGPLMEIVRTLSSDNGSPLFYFLLHGWIQLFGDGEYILTALTVLLDCAALTAVPFLLR
                     ELFTDSRIRIAGTACAAFCLPCLHQATNLRYYSLMVLCTVLSWLFFYRALRRGTARDG
                     MLFVLFSVAGFYTHTFYLFVPLSQFFVLAIFYRRRLQAGFMISLFLLAGVTLWLPSLA
                     LQVAGYLNGSTPETVPELMRYNGAAGAYLLSISSQMLAIDSMKILRMGVAGALWLFVL
                     IQLLRNRQHTADDRHARELFAAHFLSVGIIVLISMVRPIFWLDKMDLIGLPLVCALTG
                     YAVSKIRFPAAVLAVLIAVNLAGAARYTGWRVFGKLTEQRDVIARLGAELSPEDAVVE
                     TGISHFTAEYYLKQMKIQTGPRYVFPEAQMQRPSCIDPHKLLADQRELNLEAAVLMQR
                     LKKGTGRIYVFHSPYEGLAPLYNQLNEAFSLERIIPVRQHPWGPVFTQVDVYLRR"
     gene            complement(3564..3962)
                     /locus_tag="PKI68_02885"
     CDS             complement(3564..3962)
                     /locus_tag="PKI68_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52671.1"
                     /translation="MVMRGRYVLFLGLAALLFSATAVRAEDNAPLKIPDVKADAAKAK
                     KDAEHKMHAAKKEMAGVTNAVKKDAESKMKEAKEKAAEAQKEQAKKTEEMKKEAAKGS
                     EKGQAMREEHSKKWWKFWGEKEPATAAPAK"
     gene            complement(4059..4550)
                     /locus_tag="PKI68_02890"
     CDS             complement(4059..4550)
                     /locus_tag="PKI68_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HNX52672.1"
                     /translation="MRKVSEVDWDRWDPKERATLMFILQDGKMLLIHKKRGFGAGYYN
                     APGGRLEPGETPLECAIRETQEELCITPLDPVHAGTLMFQFIDGHSIHGEVFTATGFE
                     GVPTETDEAVPLWFPTDALPWHNMWADDPVWYPKMLAGERFIGRFTFDHKTMLDHELL
                     NCG"
     gene            complement(4552..>5736)
                     /locus_tag="PKI68_02895"
     CDS             complement(4552..>5736)
                     /locus_tag="PKI68_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNX52673.1"
                     /translation="YAVSMFQYQQHKQYFAQIASGFEEPAEAELISLGATEIKAAYRG
                     LHFEADLATLYRINYEARLIVRVLAPLITFDCHSDNYLHQTALKLDWSRLMTPGDTFA
                     IHAVTSNTPSLTHSQYAALKVKDAICDFFRNKTGERPSVDLQSPDLGIHLFVRNNRAT
                     ISVDASGGSLHRRGYRTVSVEAPMQETLAAAIIRLSGWDGERPLIDPMCGSGTLLCEA
                     AMHYCRIPAGYLRKQRWGFERLPDFDAAIWKKVKTAADSAMRPLPSGLISGSDINRDS
                     VRATLTNFNNLPGCRDRLNCSEKDFRSIAKLENSTIVVNPPYGVRLGDRDSAELLSKE
                     FGDFLKQRCTGCTAYVYAGDRQLLKKVGLKPEWKKELNNGGLEGILGKFDLYASPKVV
                     KA"
     assembly_gap    5737..5836
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5837..6562
                     /gene="truA"
                     /locus_tag="PKI68_02900"
     CDS             <5837..6562
                     /gene="truA"
                     /locus_tag="PKI68_02900"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNX52674.1"
                     /translation="ITIAYDGTNYAGWQVQPGKTTVQGEIERAIEHLTGKHVRIHHSG
                     RTDAGVHAKGQAAHFDLKEPVDAGRFMNSLNALLQLDVRIMKLEEVRPDFNARYNAVS
                     KEYRYFIWNGHAVPPELRLYRLHERRRLNITAMRKAAEQLVGKHDFAAFTANPRREIE
                     GTVKTIIKLTVHRTREGDVTICVTGEGFLYKMVRSITGFLLRVGIGELKPEDAERFLK
                     AGARTNEIPTVKPLGLFLWRVDY"
     gene            complement(6581..7315)
                     /locus_tag="PKI68_02905"
     CDS             complement(6581..7315)
                     /locus_tag="PKI68_02905"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNX52675.1"
                     /translation="MKKQTIPILIALLVFSAVGFCEKIQYPVGERLSYRILWGILPVG
                     RAFITCEQITENNAPYIRIRVEAKSNRMISTLYPVNDQIDCFVDPATGLPLRVEKKTT
                     EGSFVCDDTLLFDRENLSAQWQSRSASITTNYTISSNTLDVAAFLYTLRSIPFTINEP
                     KIFKIATDGYLHGLTITAEEKKQIKVGSEKTWCTRFSAIPERDNLFVRKIPGDIWVTD
                     DTRRLMVQMRTKTPVGSVSVVLDETQ"
     gene            complement(7315..8172)
                     /locus_tag="PKI68_02910"
     CDS             complement(7315..8172)
                     /locus_tag="PKI68_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52676.1"
                     /translation="MKRFFLILLCLAPTVFGENLTSFRLGQQLLADHKEEMAAIEFRR
                     FALETTIPEEQVQAYIYAAYAYIQSGSFNDAENMLRHAEKLRPDNSMIPLLYAEAAAR
                     QKSGPTALYFLDLLPSQKTETNNALAVFTARRSAEMYVRSGQIEEARFKLKTSPADES
                     ASLSALDAYASGPRKSPVVGGVLGLIPGVGYWYSGETANGFRSLILNSLFMFGMYQTA
                     QDERWAGFGIISFFEITWYTGSIYGGIDAAQRYNQNQMDRCIDGLRVPHIEPDPEITL
                     PIFQIKVVF"
     gene            complement(8169..9017)
                     /gene="yidD"
                     /locus_tag="PKI68_02915"
     CDS             complement(8169..9017)
                     /gene="yidD"
                     /locus_tag="PKI68_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNX52677.1"
                     /translation="MAQSTINRISRRKQNGILAVIAGMLFFYTSFAQELDLAAQLFSE
                     GRWEACNRECKRAEIMQQGSAPLRIQLLGAACAIRMNPADTDKIKDRLTALSMQTQDV
                     EVAAGASFEKARLLWQDDLPRQALDSFAFTFQITTNRQLFLMAACSAFLVLDKNQALK
                     TEYPALVQQINTSRALWSGELFGLCRPKKKPSRFQIDPAGWFVHFYRSQISPAIGQRC
                     TLEPSCSEYFKQATARHGWLGVPMIGDRFIREPDVNKYKTKPVLVNGMVRYADPVDDH
                     DFWMKK"
     gene            complement(8998..9297)
                     /locus_tag="PKI68_02920"
     CDS             complement(8998..9297)
                     /locus_tag="PKI68_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52678.1"
                     /translation="MKKVVAIIMVSVLMLTAFQVQAETTKIGGGRGGVMGFIAGCCFG
                     TRAGSDYNGGKEICWREWVRLIPYVNIVFAVWDGIDGANGVTRAQYAEKYGSVYY"
     gene            complement(9325..10059)
                     /locus_tag="PKI68_02925"
     CDS             complement(9325..10059)
                     /locus_tag="PKI68_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52679.1"
                     /translation="MFLFILFLASTLSATEVDRLLTEYSSIKTVSCQIRRTVSGNDGN
                     VQFASRIYWQNDNRLHVENLSPIPRRIISDGTTFFSYAQGDPKGFSRPVSALSDEMLM
                     SLHKIPGTAMDHLLRLKGHDEQLLDPAEGMSRTAYITANNYVVLRFDNLTRLIAIDFF
                     KSPDMTVKTASYHYSDFSEAAPGVWIPLLHEATLSLENGKTFSETVHIDRFIANQPVA
                     VSLFNPTAFFDKEVDFVDSFAKIYSE"
     gene            10271..10753
                     /locus_tag="PKI68_02930"
     CDS             10271..10753
                     /locus_tag="PKI68_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52680.1"
                     /translation="MRSMWLMITIVLAMGLQTVFAEEPAPMTPVMLSFANPAQLPAEN
                     YDVNGLRIDLLYGKCRNLTGLDVGLVNYATGRETGLEVGLVNNAGKSFTGLQIGAVNV
                     SDRVRALQIGLFNGADDMSGLQIGLINHTRIMRGCQIGLVNVIENNDLAFFPVINCFF
                     "
     gene            10769..12004
                     /locus_tag="PKI68_02935"
     CDS             10769..12004
                     /locus_tag="PKI68_02935"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNX52681.1"
                     /translation="MNIQKTIISAFCLIGAIASAQNTATVSQPVNPANSAELQSFKQQ
                     VQAANPGMEVVSVSAVREATLAAVPNPKIAIFVKNQTKVAGMDDMVDGVRDRISAELA
                     GAGLTVMDQAEIAAGFNRYKVTTAEERAGLIDGVFTGGSTVRVAQMLGADYVMVASII
                     SADRMSMTVGTSRTTVFTLRMSVKINEAVQGTSVYGDNWTHKYPVEEPDAAGSDPLAF
                     YNDLLDTWAQETGAKIASSMKTWRRAQAAPVQLVSFSVSTTIDELIKGLESGVRAPNE
                     LLDEMRHIVGGATVQIDGAAVGSSPGTYQVAPGLHQMRISRQWMKDWQQTVNIQNGSN
                     FRIGLELSDAGMQRFQSLEHLKAQIAKDYAEAMALKGIKVNFDTAAWRDVSVGNKGSE
                     INLEKKEIQQRGLINAAGQ"
     gene            12025..13557
                     /locus_tag="PKI68_02940"
     CDS             12025..13557
                     /locus_tag="PKI68_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52682.1"
                     /translation="MFLSFKSINRTGCLLLIWLLLLTAGCATHSQNAGMAIARLKTGD
                     TSAALHWSEKLKESSRSGDLGYLESGRIKMLAGDFAGSREDFAVAIDKVLEETENGPV
                     IRAGSVGSTLAASTIADDTVRKYQLSPYEIIQLLHYQTLNYLFSGDPEGAGVEIRRTV
                     FAQDAIAEKYSKEIAKAQESVRPDQAQAMAAVQTRMQTLGPVLERTRSSYENGLAWYF
                     CGLMFEKQGDAANSSLCYRKAWELAPGNACILKDFLRMLRTQDPQTFSELTVQNNVDV
                     NSLTRGSTEIVVLYEDSMISQRQTEKIPIPIPDFRGAITLISIDFPFYTDGAYTPKPL
                     TIAEGETVLGVTEPAVYLQSLAYQDLKDKMPGIVTRNVTRAATKVIAQEIANNRNDDL
                     VRFGMMAISAATSLASTSDTRAWYSIPMVTHLYRGSVSPGTHLLQCRTVYGTTLTIPV
                     TVADGETRLVWIANTGGVEVAATASLSGKGLPPDFEQFNNPFWTNRPVSAISTGGNTT
                     GM"
     gene            13571..13993
                     /locus_tag="PKI68_02945"
     CDS             13571..13993
                     /locus_tag="PKI68_02945"
                     /inference="COORDINATES: protein motif:HMM:NF018889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1425 domain-containing protein"
                     /protein_id="HNX52683.1"
                     /translation="MKIPKHVLAALILTSGLAGLVGCRNTSGTIVNTDHLTGQSAIIE
                     ENGRLAHRVNVVKISYDEVNGLKRVHFALASDRNRRTRIDYRICWFDVNGMEIDSQTK
                     PYRNLILEGRDVQTVTGVANSPAAVSSKLRIRETSQAQ"
     gene            14010..14612
                     /gene="lpoB"
                     /locus_tag="PKI68_02950"
     CDS             14010..14612
                     /gene="lpoB"
                     /locus_tag="PKI68_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HNX52684.1"
                     /translation="MKKLVFPVCLLCCVAVLCGCQTRVHEVPLDRAAATTKLEPQDIR
                     RTVEKMVESLLVAPGVKEFVSNRRPVLDVEPMQNRTTQHVDMVSLTESVRMQLLRSGM
                     FRFVDRSSTGTAAIDVARIHQENQLGLVDSTKAVKAGQQTALDMYLTGTLSEIKNVTG
                     RTTDQYYKFSMILKDLKTGEIVWADEQEIRKESTRPAVRW"
     gene            14754..15428
                     /gene="rpe"
                     /locus_tag="PKI68_02955"
     CDS             14754..15428
                     /gene="rpe"
                     /locus_tag="PKI68_02955"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNX52685.1"
                     /translation="MPKIEIMPSILAADMGNLEAAVRQCEKAGADQIHVDVMDGVFVP
                     NISMGPAVVAMADRVTDLPLDVHLMLIQPQNHIEAFAKAGADTILIHIEADCDVEATL
                     KKIRGLGCKSGITVNPPTPVESIFQCLEKELADEVLIMSVNPGFGGQKYIPEAEAKVA
                     AIRRRWPDLLISIDGGIDLETVKPAAAHGANLFVAGTALFKAPDMNTAVTQMRASAEA
                     AYCSKI"
     gene            15442..17247
                     /gene="lepA"
                     /locus_tag="PKI68_02960"
     CDS             15442..17247
                     /gene="lepA"
                     /locus_tag="PKI68_02960"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNX52686.1"
                     /translation="MSELSHIRNFCIIAHIDHGKSTLADRILQLTGAIEARKMKEQVL
                     DAMDLERERGITIKAHPVTMKYTAQNGQVYTFNLIDTPGHVDFSYEVSRSLQACEGAI
                     LVVDAAQGVEAQTVSNAFLAAEHNLTIVPVLNKVELANAQPEVVARQIEELLAIDATN
                     CLQVSAKTGKGISELLEAVVERMPPPKPAADNAVRGLVFDSKYDAFRGVVVYMRLFSG
                     NLKAGEKVRMMSTGREYEIKEVGVFMPDPEVCKELTEGSVGYVIANIKDSSEIQIGDT
                     MTTVRNPAADALPGFKEIHPMVFAGIYPIETSDYEKLGKSLDKLRLNDAAFSYQSESS
                     VALGFGFRCGFLGLLHMEIVQERLRREFDLDIIASYPNVVYRIHLKNGDVMEIDNPTY
                     LPDPTVIETMEEPFIKATIITPTVCLGEMMKLVMEKRGEITHTDSIDAQRVMLTCDLP
                     LNEVLIDFYDRLKSLSRGYASMDYEYADYRLSDLVKMDILIHGEPVDAFSSIVHRSKA
                     VPRGRQMCESLKDVIPKQAFSVAIQAALGGKIIARETIQAYRKDVTAKCYGGDISRKR
                     KLLERQKEGKKKMKSIGKVNIPQEAFIAVLKTKQE"
     gene            17252..>18375
                     /gene="lepB"
                     /locus_tag="PKI68_02965"
     CDS             17252..>18375
                     /gene="lepB"
                     /locus_tag="PKI68_02965"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNX52687.1"
                     /translation="MTIFKHRNERKELKEILHYAKHIRHMHEDIADPQALEQLRTGEQ
                     IGRVVRKTGAGDQMKAAGAAIIKACEKVAPPRRQPKVRENVEVLFVAIAAAMAIRAYF
                     FQPFKIPTGSMQPTLYGITVKNEPTLKSDNPLAFIANMVLFGERHVVVKAKTSGFVRT
                     INNGTRFDLQTTKSQSEAVFFINNLPHKVPMNLWHEQENHFQSVANRYINEGEILFDG
                     VIKSGDYILVNRIKYNFIRPKRGDIAVFDTRELTNPQVRRDAYYIKRMVGLPGETISI
                     APKYLLVNGEKPTDHRFDKIFNDPKYKGYQFADSRAVPPPLIDKPGDSITTAADQYLF
                     FGDNTLNSLDGRYFGAVNRQQILGPAFFVCWPFDRAGFADG"
BASE COUNT         4290 a         4656 c         4948 g         4381 t
ORIGIN      
        1 gcccagatcg ccggatcggg cgtttccaga atcaacggca tgttgtcgaa ccgtttgtcg
       61 gccatcagaa agcggaagca gtcgaggccg ataaatcctt tgccgaggca ttcgtgccgg
      121 tccacccgcg tgccgagctc ttttttggag tcgttcaggt gcattccttt gagatacttg
      181 aagccgacga tccggtcgaa ggcttcgaag gtttcagcac acgctttttc ggtgcgcagg
      241 tcatagcccg cggtaaacgc atggcaggtg tcaatgcaga ctccgacgcg cgttttgtcg
      301 tggatctgtt cgatgatttc cgcgagctgc tcaaagcgat agcccatgtt tgagccttgc
      361 cccgccgtgt tttcaatcac tgcgatcacg ctcttagttt tggaatgggc gagattgatc
      421 gactccgcaa tcaaggcaat gcactcgctt tccgagcatt ttcccagatg gctgcccgga
      481 tgaaaattca gccggtcgag cccgatctgt tcgcagcgct gcacctcatc cagaaaggca
      541 gcgcgcgact tttcgagtgc ttcgggttcc gggctgccga ggttgatcag atagctgtcg
      601 tgcggcagaa tttgcgcggc ggcaaaagag ccggcgcagt tctggttgaa cgcccggata
      661 ctttcgccgg agagcggggc ggaggtccac tgccgctggt ttttcgtaaa cagcgcaaag
      721 gcattcgcgc cgattgcttt ggcgttgagc ggcgcgtttt cgacaccgcc actcgcgctg
      781 acatgagctc cgatggtttt catgccggtc attttatata aaaaacggca ggctccaaca
      841 ttccgtgcac aaaatattcg tgattagaca gaatccggct ttagtcagag gtatcccacg
      901 gcagtctgat tcctgacttg cagatcgcac agggataatt cggatacctt tcagaagaaa
      961 tacggtcagt ttctgctgac gcagtttagt tctgttgccg cacacaaaag cgaggctgcg
     1021 cattacgcag actcttaata attacggtgc gtttttaaag cattggaaat tgaatcacgg
     1081 caaagcgcgg tttattctgc ccgtgtttcc acggggaatt tttcctgcaa attttgcagg
     1141 cagtttccgc ttatatgcag cagagccgga agctggatgt tgacgaagct gcaaaaccag
     1201 gaggaatgcc gaatgaagaa atggacagcc ggtgtgttgg tgatagggct ctcgacagcg
     1261 ctcctgtccg gatgcgcaag cgttcagaca tcgaagaacc tcaatgggca gaaaattgat
     1321 gccgccggaa ctcccgtggc tcacatccat gcaaagaaca gcggatatta ccttcttggg
     1381 attccgctga ttgccggtca aacggaagcg ggctctgtag gtaaaatcgg ccttggcaaa
     1441 gacactgtca atgtcagcag tatggttgat gtcgttacgc gcgaaagcaa agcgctgggc
     1501 ggaacaaaaa cagttgatct atcctcgcgc gtcgacagta tgccgatgct gccgatcccg
     1561 tttgttttca ctatcgagag cgttcaggtt tcaggaaatt ctgtgaaata aaaaccggat
     1621 atacagctgc cccgctcatt cgggcggggc agctgcattt tatgcccgaa aatctgcggc
     1681 gcgcactttc aaacgattga tgaatcagtt aaattccgtc gcaaccggga ggacatgatg
     1741 gataagcata ttatcgttgg cgttcatatt acggaacgga caaaacatgc agcagaaatc
     1801 caaaaggtat ttacggaatt cggctgccag atccgtacac ggcttggcct gcatgaagcc
     1861 ggcaagggtt cctgctcgcc caacggaatg attctgctcg atatggtgga cgatgacgcc
     1921 aaggtcggcg cgttcaaaaa aaagctgctg gcgatcgacg gcgtcgaggt tcagtcaatg
     1981 gtttttgatc atgcgtaagg acgggaaaca cgttgaacgg tggctgattc ctctgctggt
     2041 gctggcaggt gccgcgttac ggctgataca gctgccgttt tcctgtatct ggctcgatga
     2101 ggcgttttcc atcaggttgg cgcaaggacc gttgatggag attgtccgca cactcagttc
     2161 ggataacggc agtccgcttt tctatttcct gcttcacggc tggatacagc tgttcggcga
     2221 cggcgagtat atactgacag ctctgaccgt tctgctggac tgtgcggcat tgacagccgt
     2281 tccgtttctg ctgcgagaac tgttcacaga ttctcgcatc cggattgccg ggacggcctg
     2341 tgctgcgttc tgtctgccgt gtctgcatca ggcgaccaat ctgcgttatt attcgctgat
     2401 ggttctctgt accgtgctgt cgtggctttt tttctaccgg gcactgcgcc ggggaacggc
     2461 gcgtgacgga atgctgtttg tcctcttttc ggttgcagga ttttacaccc atacctttta
     2521 tctgtttgtg ccgctctctc agttttttgt tttggcgatt ttttaccgcc ggcggttgca
     2581 ggccggattt atgatctcgc tgtttctgct ggcgggcgtg actctttggc tgccgtcgct
     2641 ggccctgcag gtggctggat atctcaacgg ttcaacgccg gaaaccgttc cggaactgat
     2701 gcggtacaac ggggctgccg gagcgtatct gctcagcatt tccagccaga tgctggcgat
     2761 tgattcgatg aaaattctgc gcatgggcgt ggcgggggcc ctttggctgt ttgttctgat
     2821 tcagcttctg cgtaaccggc agcatactgc cgatgaccgc catgcccgtg aactttttgc
     2881 cgcgcacttt ttatccgtcg gcatcattgt gctgatttca atggtccggc cgattttctg
     2941 gctggataaa atggatttaa tcggtctgcc gctggtctgt gcgctgaccg gttatgccgt
     3001 ttcaaaaatc cgctttccgg cggcggtgct tgcggtactg attgccgtga atctggcagg
     3061 cgccgcccgc tataccggct ggcgggtttt cggaaagctg acagagcagc gtgatgtaat
     3121 tgcgcggctc ggtgcggaac tgtcgccgga agatgctgtg gtggaaaccg gaatcagcca
     3181 tttcacagcg gagtactatt tgaaacagat gaaaatccag accggtccgc gctatgtttt
     3241 cccggaagcg cagatgcagc gtccatcctg catcgatccg cacaaacttc tggcagatca
     3301 gcgggaattg aatctggaag ctgccgttct gatgcagcgt ttgaagaagg gaacaggccg
     3361 gatttatgtc ttccacagtc cgtatgaagg actcgcaccg ctctataatc agctgaatga
     3421 agctttttca ctggagcgga ttatccccgt ccgccagcat ccctggggac cggttttcac
     3481 gcaagtggat gtatacctcc gccggtaaaa gaacagcccg gagtatttca aagaatattc
     3541 cgggctgttc ggtttgttcc ggtttattta gcaggtgctg ctgttgcagg ttctttttcg
     3601 ccccagaact tccaccactt cttgctatgt tcttcgcgca tggcctggcc tttttcagag
     3661 cccttggcgg cttctttctt catctcttca gttttttttg cctgttcttt ttgcgcctct
     3721 gcggcctttt ctttggcctc tttcattttg ctttcggcat cttttttaac agcattggtg
     3781 acaccggcca tttctttttt ggcggcgtgc attttgtgtt cagcatcttt tttcgcttta
     3841 gcggcgtccg ctttaacatc aggaattttg agcggcgcat tgtcttcggc tcttacagcg
     3901 gttgctgaaa aaagcagagc agccagtcca aggaataaca cataacgacc acgcataacc
     3961 atacctcctg tttgcgatgc agacaccatg tctgcgccga tgccggcatg ataatcatcc
     4021 gtccggaagc tgtccagagg gggagcccgg ttttttggtc agccgcagtt cagtaattcg
     4081 tgatcaagca tggttttgtg atcgaacgtg aagcgtccga taaatctttc accggcaagc
     4141 atctttggat accagaccgg atcgtcggcc cacatattat gccagggtag agcatcggtc
     4201 ggaaaccaga gcggaaccgc ctcgtcggtt tcggtcggca caccttcaaa tccggtggcg
     4261 gtgaaaactt cgccgtgaat actgtggccg tcaatgaact gaaacatcag cgtaccggcg
     4321 tgtaccggat caagcggagt gatgcagagt tcttcctgcg tttcgcggat cgcgcattcc
     4381 agcggcgttt cgccgggctc aaggcgtccg cccggcgcgt tgtaatagcc ggcaccgaaa
     4441 ccgcgttttt tatgaatgag cagcatcttt ccatcctgca ggatgaacat cagcgtcgcc
     4501 ctctctttcg gatcccagcg gtcccagtca acttccgaaa ccttgcgcat atcacgcctt
     4561 cacgactttc ggcgacgcat acagatcaaa ttttcccaga atgccttcga gcccgccgtt
     4621 gttgagttct tttttccatt ccggctttag gccgactttt ttgagaagct ggcggtcgcc
     4681 ggcatagacg taggccgtac atccggtaca gcgctgcttg aggaagtctc cgaattcttt
     4741 cgacagcagt tcggcggagt cgcggtcgcc cagacgcacg ccgtagggcg gattgactac
     4801 gatggtggag ttctccagtt tcgcaatact ccggaaatct ttttccgaac agttgagccg
     4861 gtctctgcat cccggaagat tattaaagtt ggtgagcgtt gcgcggacag agtcgcggtt
     4921 gatatcgctt ccggaaatca gtccggaggg cagcgggcgc atagcggaat cggccgcagt
     4981 ttttactttc ttccagatgg cggcgtcaaa atccggcagg cgttcaaagc cccagcgctg
     5041 tttgcgcaga tagccggcgg gaatccggca gtagtgcatg gccgcttcgc agagcagggt
     5101 gccggaaccg cacatcgggt cgatcagcgg cctctctccg tcccagccgg agagccggat
     5161 gatggcggcc gccagcgttt cctgcatcgg cgcttcgacg gaaaccgtgc ggtagccgcg
     5221 gcggtgcagt gagccgccgg acgcatcgac actgatggta gcgcggttgt tgcggacaaa
     5281 aaggtgaatg cccagatccg gcgattgcag atcaacggaa ggccgttcgc cggttttgtt
     5341 gcggaaaaag tcgcagatgg cgtctttgac tttcagcgcg gcgtattgtg agtgggtcag
     5401 cgacggggtg tttgaagtga ccgcgtggat agcaaaggtg tcgcccggag tcatgagccg
     5461 gctccagtcg agcttaagcg cggtctggtg gagatagttg tcgctgtggc agtcgaaggt
     5521 gatcagcggc gcgagaacgc gcacgatgag ccgcgcttca tagttgatgc ggtagagtgt
     5581 tgccagatcg gcttcgaaat gaaggccgcg gtaggccgct ttaatttcgg ttgcaccgag
     5641 gcttatcagt tcggcttcgg cgggttcttc gaatccggaa gcgatctggg cgaaatactg
     5701 tttgtgttgc tggtattgga acatggaaac agcgtannnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnatca ccatcgctta cgacggaacg aactatgccg gctggcaggt
     5881 acagccgggt aaaacgaccg tgcagggcga aatcgagcgc gccattgagc atttgaccgg
     5941 aaaacatgtc cggattcatc attccggacg aacggatgcc ggagttcatg ccaaagggca
     6001 ggcggcccat tttgatctga aggaaccggt ggatgcaggc cggtttatga acagcctgaa
     6061 tgcgctgctg cagctggatg tgcgcatcat gaagcttgaa gaagtccggc ctgatttcaa
     6121 cgcccgctat aatgcggtca gcaaagaata ccgttacttt atctggaacg gccacgcggt
     6181 tccgccggaa ctgaggctct accgtctgca cgaacggcgc cggctgaata tcacggcgat
     6241 gcgcaaggcg gcggaacagc tggtcggcaa acacgatttt gcggcattca cggccaatcc
     6301 gcgacgcgag atcgaaggca cggtgaaaac aatcattaaa ctgactgtcc atcgcacgcg
     6361 ggaaggggac gtgacgatct gcgtaaccgg cgaaggcttc ctttataaaa tggtgcgcag
     6421 cattaccgga ttccttctgc gcgtcggcat tggcgagctg aagcccgaag atgccgaacg
     6481 gtttttgaag gccggtgcgc gcaccaatga aattcccacc gtcaaaccgc tcggcctttt
     6541 cctctggcgc gtggattatt gaccgcgttc gatctcgatg ttactgcgtt tcgtcgagta
     6601 cgacagaaac ggagcccact ggtgtcttcg ttctcatttg taccatgagg cggcgggtat
     6661 catcggtgac ccatatatca cccgggattt ttcgtacaaa aaggttgtct cgttcaggaa
     6721 tggcagaaaa gcgcgtacac catgtttttt cggaacctac ttttatctgt tttttttcct
     6781 cagcagtaat ggtcagaccg tgaagatatc catcggtggc gattttgaat attttaggtt
     6841 cattgatcgt gaatggaata gaacgcagtg tgtaaagaaa tgccgcgaca tcgagagtgt
     6901 tactgctaat cgtgtaatta gttgtaattg atgcactacg gctttgccac tgggcgctaa
     6961 ggttttcgcg gtcgaacaag agtgtatcgt cacagacgaa actcccctct gtggttttct
     7021 tttcgacacg caacgggagt ccagtcgctg gatcaacaaa acaatcaatt tgatcattaa
     7081 ccggatacaa ggttgagatc atccggttgc tttttgcttc gacccgaatg cgaatatacg
     7141 gcgcattgtt ttcggtaatc tgttcgcacg taataaaagc gcggcctacc ggcagaatgc
     7201 cccaaagaat ccggtaagaa aggcgctcgc cgacggggta ctgtatcttt tcgcagaagc
     7261 cgactgctga aaaaacgagg agtgcaatta gaattggaat ggtttgtttt ttcactagaa
     7321 gacgaccttg atttgaaata tgggcagagt gatttccgga tccggttcaa tatgaggtac
     7381 gcgcaaacca tctatgcaac gatccatttg attctggttg tagcgctgtg ctgcatcgat
     7441 tccgccatag atactgccgg tgtaccaggt gatttcaaaa aatgaaataa taccgaagcc
     7501 tgcccaccgt tcatcttgtg cggtttgata catcccgaac ataaacaagc tgttaagaat
     7561 gagcgaacgg aacccgttgg ctgtttctcc tgagtaccaa tagccaactc caggaatcaa
     7621 cccgagaacc ccgccgacaa ccgggctttt acgcgggcct gatgcatatg catccagtgc
     7681 ggaaagtgac gcgctttcat cggccgggga agttttcagt ttgaagcggg cttcttcaat
     7741 ctgtccggat ctcacataca tctctgcgct gcgacgtgcg gtgaaaactg cgagagcatt
     7801 gtttgtttct gttttctggg acggtaataa atctaagaaa taaagtgctg tcgggccgga
     7861 tttctgtctg gccgccgctt cggcataaag gagcggtatc attgaattgt ccggtctcag
     7921 tttttctgca tgccgaagca tattttctgc gtcattaaaa ctcccggatt ggatataggc
     7981 atacgctgcg taaatatagg cctgtacttg ttcttcaggt attgtggtct caagagcaaa
     8041 acgacgaaac tcaatggccg ccatttcctc cttgtgatct gccagtaact gctgaccaag
     8101 tcgaaacgag gtcaggtttt caccgaagac cgtcggggcg aggcagagca ggatcaaaaa
     8161 aaatcttttc attttttcat ccagaagtcg tgatcatcta cagggtcggc atatcgcacc
     8221 atcccgttaa caagtaccgg tttcgttttg tatttgttta catccggctc acgaataaac
     8281 cggtcgccaa tcatcggaac gcccagccaa ccatggcggg ccgttgcctg tttaaaatat
     8341 tctgagcagc ttggttcgag cgtgcatcgt tgtcctatgg ccggactgat ctgactgcgg
     8401 taaaaatgca caaaccatcc tgccggatcg atctgaaaac gtgatggttt ctttttgggg
     8461 cggcataatc cgaacagttc tccggaccaa agggcacgcg atgtgttaat ttgctgcacg
     8521 agggcgggat actccgtttt cagggcctga tttttatcca ggacaaggaa tgcggaacag
     8581 gcagccatta aaaaaagctg ccggttcgtc gtgatttgaa atgtaaatgc aaacgaatcc
     8641 agagcctgtc tgggcaagtc gtcctgccat agcaggcgag ctttttcaaa cgaagcgcca
     8701 gcggccactt ccacgtcttg tgtttgcatg gacagagcgg ttagccggtc tttgatttta
     8761 tctgtatcag ccggattcat ccgaatagca caggctgcac ccagaagttg aatgcgaagc
     8821 ggtgcgcttc cctgctgcat gatttctgcc cgcttacatt cccgattaca ggcctcccaa
     8881 cgtccttcgc taaaaagctg ggcagccaga tccagctcct gagcaaaaga agtataaaaa
     8941 aacagcattc cggcgataac cgccagaatg ccgttctgct ttcgtcttga tatgcgatta
     9001 atagtagact gagccatatt tttcggcata ttgagcgcgc gtgacgccgt tcgcaccatc
     9061 aataccatcc catacagcaa aaacaatgtt tacataggga atcaagcgaa cccattcacg
     9121 ccagcagatt tcttttcccc cgttataatc cgagccggcg cgagttccaa aacagcaacc
     9181 agcaataaaa cccattactc cgcctcgtcc accgccgatt tttgtggttt ccgcctgtac
     9241 ctgaaatgct gtcagcatca gtacacttac cataataatc gcaacaactt tcttcattct
     9301 gaattctcct tgtgaccaaa gtaattactc gctgtaaatc tttgcgaacg aatcaacaaa
     9361 gtcaacctct ttgtcaaaaa aggcggtagg attaaacaat gaaacagcta ccggttggtt
     9421 tgcaataaac cggtcgatgt gaacggtctc tgaaaacgtt tttccatttt caagcgacag
     9481 ggttgcctca tgaagtagag gtatccaaac acccggggcg gcttcggaaa agtcagagta
     9541 atgatatgaa gcggttttca ctgtcatatc tggactttta aaaaaatcaa ttgcgataag
     9601 acgggtcaga ttgtcgaaac ggagaacgac atagttgttt gcagtgatgt aagctgttcg
     9661 gctcattcct tcagcaggat ccagtagttg ctcgtcatgt cctttgagtc gcaacagatg
     9721 atccatggcc gtgcctggga ttttatgcaa cgacatgagc atttcgtcgg aaagtgcact
     9781 tacaggccgc gagaaacctt ttggatcacc ttgtgcgtag ctgaagaagg ttgttccatc
     9841 tgaaataatt cgtcgcggaa tcggggatag gttctccaca tgtaaccggt tatcgttttg
     9901 ccaatatatg cgactggcaa actgcacgtt cccgtcattg ccgctgactg ttcgacgaat
     9961 ttggcaggag accgtcttta ttgacgaata ttcagttaat aaccgatcca cttctgtagc
    10021 agaaagagtt gatgccaaga aaagaataaa aagaaacacg cactgtacca tgcgacggag
    10081 gctattatag aacatttgcg tcttcaaggg cgttttagcg gtatacgcgc gtctttatat
    10141 aaggaatgag ggttggatag tctgattgcg agtgcgcgga agcttgtttt cttcttgttt
    10201 tcaaaggtgt gcattcccag aatgtgtccg aattttaaat ccggctgttg cgccggttca
    10261 ggagaaaagt atgagaagta tgtggttgat gattacgatc gtgctggcca tgggcttgca
    10321 aactgttttt gctgaagaac cggcaccaat gacacccgtc atgctcagtt ttgcaaatcc
    10381 cgcgcagttg ccggcggaaa attatgatgt gaacggatta cggatcgacc tcctgtacgg
    10441 caagtgcaga aacctaaccg gtctggatgt cggtctggta aactacgcga ccggcagaga
    10501 gaccggcctt gaagtcggtt tggtcaacaa tgcgggaaaa agtttcaccg ggcttcagat
    10561 cggggcggtt aatgtatcgg accgggtgcg cgctttacag atcggtcttt tcaacggcgc
    10621 agatgacatg tccggactgc aaatcggact gattaatcat acgcgcatta tgaggggatg
    10681 tcagatcggg ctcgtcaatg tgattgaaaa taacgatctg gcatttttcc cggttatcaa
    10741 ctgtttcttc taaaactaag gagattctat gaatattcaa aaaaccatca tttctgcttt
    10801 ttgtctgatc ggcgccattg cttccgcgca gaataccgca accgtttcgc aacccgttaa
    10861 tccggcaaac tcggcggaac ttcagtcgtt taaacaacag gtgcaagcgg cgaatcccgg
    10921 catggaagtt gtttctgtaa gcgccgtacg cgaggccacg ctggccgcag tgccgaatcc
    10981 gaagattgcc atctttgtta aaaaccaaac caaagttgcc ggaatggacg atatggttga
    11041 cggagttcgc gaccggatct ccgccgaact ggccggagca ggtctgaccg ttatggatca
    11101 ggcggaaatc gccgccggat tcaaccgcta taaagtgacg acggcagaag aacgcgccgg
    11161 actgattgac ggtgttttca ccggcggttc gaccgttcgc gttgctcaaa tgctcggtgc
    11221 cgactatgtc atggtcgctt ccattatttc agccgaccgc atgtcgatga cagtgggcac
    11281 cagccggacg acggttttca cacttcgcat gagtgtcaaa atcaatgaag cagtgcaggg
    11341 cacctcggtc tacggtgaca actggactca taagtatccg gttgaagaac cggatgccgc
    11401 cggcagtgat cctctcgcat tttacaatga tcttttggac acatgggctc aggaaaccgg
    11461 cgctaaaatc gccagcagca tgaaaacctg gcggcgcgcg caagcagcac cggttcagct
    11521 ggtgagtttc tcggtcagta cgaccattga tgaattgatt aaaggattgg aatccggtgt
    11581 acgcgccccg aatgaactgc ttgatgaaat gcgccacatc gtcggcggtg caacagttca
    11641 aatcgacggc gcggctgtcg gctcatcgcc gggaacgtat caggtcgctc cgggactgca
    11701 ccagatgcgg atttcccggc agtggatgaa ggactggcag cagaccgtca atattcaaaa
    11761 cggcagcaat ttcagaatcg gactggaact ttccgatgcc ggaatgcaac ggtttcaatc
    11821 gttggaacat ctgaaagcac agatcgccaa ggactatgcc gaagcgatgg cgcttaaagg
    11881 cattaaagtc aacttcgata ccgccgcatg gcgcgatgtt tcggtcggca ataaaggcag
    11941 tgagatcaat ctggagaaaa aagaaatcca gcagcggggg ctgattaatg ccgccggaca
    12001 gtgattgcaa aatggagtca ctgcttgttt ctgtctttta aatccattaa ccggaccggc
    12061 tgtctgctgc tcatctggct gttgctgctg acagccgggt gcgcaacgca cagccagaat
    12121 gccggaatgg caatcgcccg tttgaaaacc ggcgatacat cggcggcttt gcactggtcg
    12181 gaaaagctga aggaaagttc gcgcagcggg gatctcggct atctcgaaag cggacggatc
    12241 aaaatgctgg ccggcgattt cgccggcagt cgcgaagatt tcgccgttgc catcgacaag
    12301 gttcttgagg aaaccgaaaa cggcccggtt atccgggcgg gatctgtcgg ttcaacactt
    12361 gccgcttcga caatcgccga cgatacggtt cgcaaatacc agctttcgcc gtacgagatt
    12421 attcagctgc tccattacca gacactgaac tatctgttta gcggcgatcc cgaaggcgcc
    12481 ggtgtcgaga tacggcgaac ggtatttgcg caggacgcca tcgccgaaaa gtattccaag
    12541 gaaatcgcga aggcgcagga aagcgttcgt ccggatcaag cccaagcgat ggcggctgtt
    12601 cagaccagaa tgcaaacgct ggggccggtg cttgagcgga cgcgcagttc ctatgaaaac
    12661 ggactggcct ggtatttttg cggactgatg tttgaaaagc agggcgatgc ggcgaattct
    12721 tcactctgct accgcaaagc gtgggagctg gctccgggta acgcctgcat actgaaagat
    12781 ttcttgcgga tgctccgcac gcaggacccg cagacatttt ctgaactgac ggtacagaac
    12841 aatgtggatg taaacagcct gacacgcggc tcaacagaaa tcgtcgttct ctatgaggat
    12901 tcaatgattt cgcagcgtca gacggaaaaa attccaattc cgattcccga tttccgcggc
    12961 gcaattacac tcatatcgat cgatttcccg ttttataccg acggggccta taccccgaaa
    13021 ccgcttacca tcgctgaagg cgaaacggtt ctcggggtca ccgaaccggc ggtttatctc
    13081 cagtcgctcg cttatcagga tttgaaagat aaaatgcccg gcatcgttac ccgcaatgtt
    13141 acccgtgctg cgaccaaggt aattgctcaa gagatcgcca ataatcggaa tgacgacctc
    13201 gtgcggttcg gaatgatggc gatcagcgcg gcaacatcgc tggcttctac gtccgatacc
    13261 cgtgcgtggt actcaatacc gatggtaact catctttacc gaggttccgt ttcgcccgga
    13321 acacatctgt tgcagtgccg cacagtttac ggcacaacgc tgaccattcc agtgacggtc
    13381 gccgacggcg agacacggct ggtgtggatt gccaacaccg gcggcgttga ggttgcggcg
    13441 accgcctccc tgtcagggaa agggctgccg ccggactttg agcagtttaa taatccgttt
    13501 tggacaaaca gaccggtttc tgcgataagt accggcggaa acaccaccgg gatgtaaagg
    13561 agactatact atgaaaattc caaaacatgt actcgcagcc cttattctga cttccggtct
    13621 ggccggattg gttggctgcc ggaacacatc cggaaccatt gttaatacag atcatcttac
    13681 gggacagtcc gcgatcatcg aggaaaacgg ccggctggcc caccgggtga atgtcgtcaa
    13741 aatcagctac gatgaagtca acggtttaaa gcgggttcac tttgcgctgg cctccgaccg
    13801 caaccgccgg acgcgcatcg actaccggat ctgctggttc gatgtcaacg gcatggaaat
    13861 tgacagtcag accaaaccgt accgcaatct gattctggaa ggtcgtgacg tgcagacggt
    13921 aaccggtgtg gcgaactctc ccgccgcggt ttcctccaaa cttcgtattc gtgaaacatc
    13981 tcaggctcaa taaagaaagg attatgcaga tgaaaaaatt agtttttccc gtgtgtttgt
    14041 tgtgctgcgt tgctgtgctg tgcggttgcc aaaccagagt tcatgaagtg ccgctggatc
    14101 gcgcagccgc taccaccaaa ctcgaaccgc aggatatccg ccggacggtt gagaaaatgg
    14161 tggagtctct gctcgttgct ccgggtgtta aggagtttgt cagcaaccgg cgcccggtgc
    14221 tggatgtcga accgatgcag aaccgaacga cgcagcatgt tgacatggtc agtctgacgg
    14281 aatccgtacg catgcagctg ctgcgctccg gcatgttccg ctttgtcgac cgttcttcga
    14341 ccggaacagc ggcaattgat gtagcgcgta tccatcagga aaatcagctg ggactggttg
    14401 attcgacgaa ggcggtaaag gccggacaac agaccgcgct ggatatgtat ctgaccggta
    14461 cgctgtcgga aattaagaac gtaacgggtc gcacaaccga ccagtactat aagttctcca
    14521 tgatcctgaa ggatcttaag accggagaaa tcgtctgggc ggacgaacag gagatccgca
    14581 aagagagcac ccgtccggca gtccgctggt aaagtgcaaa ggtgccctgc aagacccgcg
    14641 cggatttcct gtgcgggttt tttgtgccgc acaccggcga ataaaccccg ctcttcttgc
    14701 tttttgcaaa cgggttcggt agcttctctg ctcacgtttt cacagggaag aatatgccga
    14761 aaattgaaat tatgccgtcg attctagcgg cagacatggg gaatctggaa gccgccgtcc
    14821 ggcagtgcga aaaggcagga gctgatcaga ttcacgtgga tgtgatggac ggagttttcg
    14881 tgccgaatat cagcatgggc ccggccgtag tcgccatggc tgaccgcgtg acggatcttc
    14941 cgctggatgt tcatctgatg ctgattcagc cgcagaatca tatcgaggcg tttgccaaag
    15001 ccggtgcgga tacgatcctg attcatattg aagccgactg cgatgtggaa gcgacgctca
    15061 aaaaaatccg cgggctcggc tgcaaaagcg ggattaccgt taatccgccg acgccggtgg
    15121 aatccatttt tcaatgcctc gaaaaagaac tggccgatga agttctcatc atgtctgtaa
    15181 accccggctt cggcgggcag aaatatattc cggaggccga ggccaaagtg gcggccatcc
    15241 gccgccgctg gccggatctg ctgatttcga ttgatggcgg cattgatctt gaaaccgtaa
    15301 aaccggcggc ggcgcatggt gcaaatctgt ttgttgccgg cacggcgctt ttcaaagcgc
    15361 cggacatgaa tacagccgtc acccaaatgc gcgcttcggc tgaagcggcc tattgttcaa
    15421 aaatctaacc gaaaatctga catgtctgag ttatcccata tccgcaactt ttgtatcatc
    15481 gctcacatcg accacggcaa atcgacgctg gccgaccgta ttcttcagct gaccggcgcc
    15541 attgaagccc gcaagatgaa agaacaggtg ctcgacgcca tggatcttga acgcgagcgc
    15601 ggcatcacca tcaaagcgca cccggtgacc atgaagtaca cggcccaaaa cggacaggtc
    15661 tatactttca acctgatcga cacccccggc catgtggact tttcttatga agtttcccgc
    15721 agtcttcagg cgtgcgaagg cgccattctc gttgtggatg cggcgcaggg cgtcgaggcg
    15781 cagacggtct cgaacgcctt tctggcagca gaacataatc tgacgattgt gcctgttctg
    15841 aataaagtcg aactggcgaa tgcgcagccg gaagtggttg cccggcagat cgaagagctg
    15901 ctggcgattg acgccacgaa ctgcctccag gtgagcgcga aaaccgggaa ggggatatcc
    15961 gaacttctcg aggccgttgt ggaacgtatg ccgccgccga aaccggcggc agacaacgcg
    16021 gtgcgcggac tggtctttga ctccaaatac gatgccttcc gcggtgtcgt ggtgtacatg
    16081 cgtctgttca gcggcaattt gaaagccggt gaaaaggtgc gcatgatgag caccgggcgc
    16141 gagtacgaaa ttaaagaagt cggcgtgttt atgccggatc ctgaagtctg caaagagctg
    16201 accgaaggtt ccgtaggcta tgtgatcgcc aacatcaaag actcttcgga aattcagatc
    16261 ggcgatacga tgaccaccgt ccgcaacccg gcggcggatg cgctgcccgg ttttaaagaa
    16321 atccacccga tggtctttgc cggtatttat ccgattgaaa ccagcgacta cgaaaagctc
    16381 ggcaagagtc tggataagct gcgcctgaac gatgccgctt tttcctatca gtcggaatcc
    16441 tccgtggcat taggtttcgg tttccgctgc ggctttctcg gcctgctgca catggagatt
    16501 gtgcaggaac ggctccgccg agaattcgac ctcgacatca tcgcgtccta tccgaacgtc
    16561 gtgtatcgca ttcatcttaa aaacggcgat gtgatggaaa tcgataatcc gacctacctg
    16621 ccggatccga cagtgatcga gaccatggaa gaacccttca tcaaagccac catcatcacg
    16681 ccgacggttt gtctggggga aatgatgaaa ctggtgatgg agaaacgcgg cgaaatcacg
    16741 cataccgatt cgatcgatgc acagcgcgtc atgctcacct gcgatctgcc gcttaacgaa
    16801 gtgctgatcg atttctacga ccggctcaag agtctcagcc gcggctatgc gtcgatggat
    16861 tatgaatatg ccgactaccg gctctcagat ctcgtcaaaa tggatatcct gatccacggc
    16921 gaaccggtcg atgcgttttc gtcgattgtt caccgttcga aagcggttcc ccgcggacgg
    16981 cagatgtgcg aatcgctgaa ggatgtcatt ccgaaacagg ctttctctgt tgccattcag
    17041 gcggccctgg gcggtaaaat catcgcccgc gaaacgattc aggcctaccg caaagatgta
    17101 accgccaagt gctacggcgg cgatatttcc cgtaagcgca aactgctcga gcgccagaaa
    17161 gaaggcaaaa agaaaatgaa gagcatcggc aaggtgaata tcccgcagga agcgtttatc
    17221 gcggtgctca aaaccaagca ggaataagct catgactatt tttaaacacc gcaatgagcg
    17281 caaggaactc aaagagatct tgcattacgc caaacacatt cggcacatgc acgaagacat
    17341 tgcggacccg caagcgcttg aacagctgcg taccggggag cagatcggcc gcgttgtacg
    17401 caaaaccggg gcaggggatc aaatgaaagc ggccggagct gcaatcatca aagcctgcga
    17461 aaaagttgcg ccgccgcgcc gccaaccgaa agttcgcgaa aatgtagagg tcctcttcgt
    17521 tgccattgcc gccgccatgg cgatccgcgc ctacttcttc cagccgttta aaatcccgac
    17581 cggttccatg caaccgacgc tttacggtat tacggtcaaa aatgagccga ctctaaaatc
    17641 cgacaatccg ctggcattta ttgcaaacat ggtccttttt ggagaaagac atgttgttgt
    17701 aaaagcaaaa acaagtggtt ttgtgcgcac cattaataat ggaactagat tcgatctgca
    17761 gactaccaaa agtcagtcag aagctgtttt ttttatcaac aaccttccac ataaagtgcc
    17821 gatgaatttg tggcatgagc aagaaaacca cttccagtcg gtcgccaaca ggtacatcaa
    17881 tgaaggagaa attctgtttg atggtgtaat taaaagcggc gattacattc tggtcaaccg
    17941 gatcaaatat aactttatcc ggccgaagag gggggatatt gctgtctttg acacccgtga
    18001 gctgacgaat cctcaagttc gtagagacgc ctattacatc aagcgcatgg tcggtctgcc
    18061 gggagaaacg atttctatcg ctccaaagta tctgctggtc aacggtgaaa aaccaaccga
    18121 ccatcgtttt gacaaaattt tcaatgaccc caaatataaa gggtatcagt ttgccgactc
    18181 ccgagctgtt ccgccgccgc tgattgataa gccgggcgac tccattacaa cagccgctga
    18241 tcaatatctc ttcttcggtg acaacacact gaacagtctc gatggacgct atttcggagc
    18301 cgttaaccgc caacaaattc tcggcccggc attctttgtc tgctggccgt ttgatcgcgc
    18361 cggatttgca gacgg
//
LOCUS       DAOPZO010000024        18190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_3218_length_18190_cov_6.427075, whole genome shotgun sequence.
ACCESSION   DAOPZO010000024 DAOPZO010000000
VERSION     DAOPZO010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 18190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18190
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3218_length_18190_cov_6.427075"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..74
                     /locus_tag="PKI68_02970"
     tRNA            1..74
                     /locus_tag="PKI68_02970"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33..35,aa:Gly,seq:tcc)
     gene            complement(1454..1981)
                     /locus_tag="PKI68_02975"
     CDS             complement(1454..1981)
                     /locus_tag="PKI68_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52688.1"
                     /translation="MATEFPFEEIPAKLKAIIGDPDPGTRIYRAEGGQEEHAKWFKAV
                     HQICTEGMVSPGGAGHFAPVSRAAVYKALREGRLSAFFFHTTKTYFSWMGKEFKHRDT
                     PYGYVSVRELKAWKENLEARAQRIEAIEERRALELEIYGTTKDERGKEIYYYPKDRIK
                     AEKAKAKAKKKGNTT"
     gene            complement(2037..2738)
                     /locus_tag="PKI68_02980"
     CDS             complement(2037..2738)
                     /locus_tag="PKI68_02980"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNX52689.1"
                     /translation="MQKRFTYEQVAFDYALTQESRKTLAITVFPDQSVRVKAPLHATE
                     EKINGFLQRKFRWVLKHQRYFSRFKPRARKEYIGGETFRYLGRNYKLLIRKAEEQERV
                     SLQQGTLTVLSLFPQNRLHTKKLLDAWYAGKARQHFAERLDHCAAQFALKTRPGLVIR
                     SMTRRWGSYSRKTGRICLNLALIKASRHQIDYVLTHELCHITHTAHDKAFYRLLSSHL
                     PDWKKVKAELEQSLV"
     gene            complement(2738..5923)
                     /locus_tag="PKI68_02985"
     CDS             complement(2738..5923)
                     /locus_tag="PKI68_02985"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNX52690.1"
                     /translation="MKPEFELPDFDEASSSQIPALLELVNLGYTYISRADVAKLRESS
                     SQYILRTIAFDALRKINGPNISDKSIRDEIFKAEQQIDMGAGVFKASEEIYSLLLAGC
                     GVSELIDGRRTSPQMKFIDWRNPANNTFHVCAEFELSEGSDRRPDIVVFVNGIPFAVI
                     ENKKSSVKVAEAVAQMVRNQGRTETPKFFIFPQLLIATNGRELKYGTMLTPSRFYSHW
                     KEKDTDPAAFDAKVSAIVNKPVESETVAQIAQDLIRSDYRQNERTVTEQDRGVYSLLR
                     PERMLDLVRNFILYDKGIKKVARYQQYFAIRKTMDRLKTFDEYGRRRGGLIWHTQGSG
                     KSLTMVMLVKCLIDDPEIVLPRVLVVTDRRDLDRQIADTFAACNIKKDVKKTTSSGEL
                     LQLIKEKDQRVITALIHKFEAGRAARDFVDNDPNVFVLIDEAHRSQAGQANTELNLIL
                     PKACQIGFTGTPLMRKEKSSAAKFGGIIDAYTISEAEADGVVLPLIYQPRYVELSVQQ
                     KLLDQFYDTITKDLSEGEKKDLQQKFSSSQIIEETSQRIETIALDILEHYSRFADTGL
                     KAQVVAPSKYAAVMFQKAFSLQDRIQTDVVISESADKEEDDQLAEHKKEVVKFLAEEK
                     HKHGSSLLKREKDLIDDFKENPDGVRMLIVVDKLLTGFDAPRNTFLYLAKQLKDHNLL
                     QAIARVNRLFDGDRGKEAKVNGFVIDYSKNAKNLRDAMELFSNYDPEDIERALLNTDE
                     KIAGLERVYQELHGIFNSVRNKQDTEEYIRILEDDIETREHFYELVDEFTRQFSTCQM
                     LYDFTQKFDEEKLRRFALDLKRFVELKKIQKIKNAETVDFSKYEDQIRRILDKYVSAE
                     YVTELAEPICVSESAEFNAYIEKAERGLSDKSKAEAIAAQTERTIKENYSKDPEFYRH
                     FSEKISRLIAELRSAKEEDVKALLGEVRQVQGQVADYEDSDIPAPLKERKPLHPYFRN
                     LKKRLAEHPLTGEQLCSVVETIYQLIEQHKIVDWDSDIEVERRVRMEIEDYLFDTVKN
                     KYGALLTFDEIDAILTLVWDLAVQNKDEPGL"
     gene            complement(6405..7112)
                     /locus_tag="PKI68_02990"
     CDS             complement(6405..7112)
                     /locus_tag="PKI68_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52691.1"
                     /translation="MKLLNVYEIRARYIPAIIAALPAIILSAFIKQDVWISLFENARC
                     FLIVENLSLSLIAILFLIHVQRGIAKHLFENRIFKAGKEFPTTTMLLLSDSFFSVEMK
                     NKIRGKIETDFGIRLCPLEQEQANIEEAKKTIRDAVNLIRKQVKDGDKTLQYNIHYGL
                     SRNLIAGSIFAIPVSVLNAVLFGIHNPAGFYISLLMTAFFSLTLIFNRKILIHYASAY
                     AECLLSEFLTTKGANHD"
     gene            complement(7124..8203)
                     /locus_tag="PKI68_02995"
     CDS             complement(7124..8203)
                     /locus_tag="PKI68_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX52692.1"
                     /translation="MSTLGELQKAYDQVVASSYNGLEIFMMNIGNADCFLVIRYYPGG
                     TKDVVLIDGGNKSDAVDIAKHLTGLGVTHVNHIVNTHSHDDHTGGLIELIKGDTFTYD
                     NLWMPQAWNHIDWQGIKDQLGRNSAKWVLERFQRSLTSQAELYNLSSSKGITPQEPFA
                     GTTIGPFLVLGPTLEFYVECLSSFGDVEKVRQWNDYLERKNTSSALIEAISEILEEDS
                     GTLGGITSPENESSVVLATTLGEDKILFTGDAGCDAFSSIIYNDRFRHLQNIHWMQAP
                     HHGSRRNLSEDLVKLFKSKVVFISAKGSQKHPSRKLVNCLKDNGATVYSSHYPAGNKF
                     TWIRQTIGTVPSRQISPAAALYEAD"
     gene            complement(8225..9739)
                     /locus_tag="PKI68_03000"
     CDS             complement(8225..9739)
                     /locus_tag="PKI68_03000"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HNX52693.1"
                     /translation="MTVKKYTQEELNKTLWNAADSSRGTVDGGVYKDYVLLLLFYKYI
                     SDLHQHTIDKLKERYGDDPDRIALRLKNERFIIPEGASFFDLFKKLEADNLGELINVA
                     LHKIEDANGSRLEGILTVDFNSDAILGKTEQRNKMLRNLMTDFAKIDMTEAGDDVIGN
                     AYMYMIERFGSDAGKKAGEFFTVKSVAQLLARLVKPQPGWRICDPACGSAGLLLLAGE
                     EIEKQGSKDYALYGQESNMGTYNLARMNMFLHGKDSARIEQGDTINNPQLKENDALMK
                     FDLVIANPPFSLKKWMGENIESDKYKRFWRGMPPATKGDYAFVTHMVETAKAKEGKVA
                     VIVPHGVLFRGAAEGRIRQSLLEENIVDTVIGLPAGLFQTTGIPVAILILDRSREPGG
                     KNESRKDVFFVEASKEFKPGKAMNTMEPANIQKILDAVEARKDIPKFCRAVTPEEIKE
                     NDYNLNITRYVDTFEEEEEVDIEANLKELKQVDTELAGLEVEMQKHLKALGIEK"
     gene            complement(9755..10306)
                     /locus_tag="PKI68_03005"
     CDS             complement(9755..10306)
                     /locus_tag="PKI68_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNX52694.1"
                     /translation="MKLQDMCEIRMGYAFRSGIKHSPAGNIPVIQPKDIDNNGILQVE
                     DVYRVEMSEVNPEHILKSGDVLLSNRGRFAATTYTGQIETCVASGALLVLTVKKDAPV
                     LPEYLTLYFNSQEGKRQLGRLDQTTTIPHISRKNLEQIDMPVPPIERQETLVALERAK
                     LLYTTLTNRKIELTNNIINQQLI"
     gene            complement(10469..11596)
                     /locus_tag="PKI68_03010"
     CDS             complement(10469..11596)
                     /locus_tag="PKI68_03010"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNX52695.1"
                     /translation="MSTFKLNPLPPFVDLETKAILKKLAEAHRHLAELKGVAASIPNE
                     NILIQTLALQEAKDSSEIENIVTTHDDLFKAQLADESVLPAAKEVAQYADALRVGFES
                     VRASRLITLNQILQIHETLEKNNAGFRKLPGTALRNRATGEVVYTPPQDHATILELMG
                     DLETFINDSNRLDADPLIKMALIHYQFESIHPFYDGNGRTGRIINILYLVANGLLNLP
                     ILYLSRFIIQNKPDYYRLLQEVRTKNAWEEWVLFMLEGIAVTARQTTALVTAIRDMML
                     DCKHRIRKQFPRMYSQDLINNLFRHPYTKISILQQELSVSRLTAARYLDKLAEAGFLE
                     KRRFGRTNYYLNVPLANLFLTLPRPPPLDEFGQIESATETR"
     gene            complement(11991..12542)
                     /locus_tag="PKI68_03015"
     CDS             complement(11991..12542)
                     /locus_tag="PKI68_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52696.1"
                     /translation="MEIPEIHAMSRIDWQFFGTLTFKKERVPTAVRIKLWFALARALA
                     KWYGVRFPKLLWVLRTEQGEVTGRTHFHCLIGGLPKTAVREPKKIQRANGMWDVNNPT
                     CHALEAKWGRLGLHKDDPQNRIARFSLYDARLNGAGYLCKSLGVETSRLTKDIYETAK
                     FDWGDNELTLSDSVIRVARTYLR"
     gene            complement(12625..12894)
                     /locus_tag="PKI68_03020"
     CDS             complement(12625..12894)
                     /locus_tag="PKI68_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52697.1"
                     /translation="MKIKIQVIGVDKYNGKHGVGHTLCCFDQDTDTRLSNSFDYNVSE
                     LDKDTFGDPEQWKNQIVEVGITELTPGFGGRIRTRGKILSVPKKG"
     gene            complement(12891..13376)
                     /locus_tag="PKI68_03025"
     CDS             complement(12891..13376)
                     /locus_tag="PKI68_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52698.1"
                     /translation="MNRSSYSKGENTSFSEPQDAGIRRFNIQSPGTDSDPYSWGYPSL
                     VALYCLLKKRSAMADSDTPGKKASAEFAEELLSGKALLGMSRTDRNPTPKDAQAMKAI
                     QRIPASQIALIAASPAAEQLSKISHKQEMFPAAVAPSDEVRGEVRRVKIPDCLEGRNR
                     T"
     gene            complement(13863..14498)
                     /gene="eda"
                     /locus_tag="PKI68_03030"
     CDS             complement(13863..14498)
                     /gene="eda"
                     /locus_tag="PKI68_03030"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HNX52699.1"
                     /translation="MNLQQKFTDAKVIAVLTIDCVENAVPLARTLLEGGICAMELTLR
                     TPQAMDCLVRIVNEVPEMLAGVGTVLRPDQVRLAKAKGAAFAVSPGTNRSVLAEARQA
                     GLFFAPGIMTPSDIEAALEYDCTLMKYFPAETSGGLAHLRNIAAPYGYLKPKFIPLGG
                     INETNLKGYLADPLIAAVGGSWIAKPEEIRNADWNTILNRARTASRLAAEV"
     gene            complement(14805..15872)
                     /gene="tsaD"
                     /locus_tag="PKI68_03035"
     CDS             complement(14805..15872)
                     /gene="tsaD"
                     /locus_tag="PKI68_03035"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNX52700.1"
                     /translation="MDEVILLGIETSCDETAAAVLVDGAVRSNIIYSQVAKHAPYGGV
                     VPEIASRDHVKKLPGIIEEAMHGSGCTFEQLDGIAVTYGPGLSSSLLIGYSAARALSQ
                     RLGIPPTGVHHHEGHIYSVFLSENPPVLDGAFPMMVLLVSGGDTKLVYMKAPGCYEIV
                     GQTIDDAAGEALDKGAMLLGLGYPGGPIIQKTAEGGNPKAVRFPRGLDNAGGEWKYDF
                     DRKLCFSFSGLKTALLYHVQNHPETLKDESAKRDLIASYQEAVADALAVRLERALEQY
                     DCASFACAGGVSLNKVLREKLNRLSEKCGVPLKLSSPRLCTDNAAMIAGAAYIKLSGG
                     HPFVLPDDVNPNLRLENWFRV"
     gene            complement(15860..17095)
                     /locus_tag="PKI68_03040"
     CDS             complement(15860..17095)
                     /locus_tag="PKI68_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX52701.1"
                     /translation="MKLKPAVFLSVLFAFAASPRAAESYTNAYDAIALFTKVLEEVHR
                     SYVDTNEAGYDSLVRHAMTGMLQDLDPYSQFLEKDSYDDMKDDTAGRFSGVGLVVSMK
                     DGLLTVVSPMEDTPAFRAGLLSGDIIVGIDGQETREMSLSDAVKKMRGEAGTHVKLKA
                     VRPSTHEVKEHDLIREKIEVASVKDAKLIEDGIGYIRITQFNEPTAEDLKKELKKLTD
                     QKMKALVLDLRGNPGGLLTAAAGVAELFLPHGDLIVFTKGRTAEMDQKPFKSSGSTHY
                     TGFPIVLLVNGGSASASEIVSGALQDHKRAVLVGEKTFGKGCVQSVLPLADGAAIKLT
                     TALYYTPSERVIQDHGIEPDVKVVMNPEDLFKIRLKQAQKDGEADKALPVAQEPRDIQ
                     LDYAIGALKGMMIQQEWMK"
     gene            complement(17152..17775)
                     /gene="thiE"
                     /locus_tag="PKI68_03045"
     CDS             complement(17152..17775)
                     /gene="thiE"
                     /locus_tag="PKI68_03045"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNX52702.1"
                     /translation="MKENFGLYMVMTDPVTGYEACAQAAVNCGLRYVQLRMKNASRAA
                     ILAEARKVRAVTVGTRTLFIMNDDVELAWAADADGVHLGQGDMPLDEARRLWNAPGKI
                     FGLSTHSEAQELAARALRPDYIGVGPVFPTPTKAIADPALGIERAGTIIKNSPLPCVA
                     LGGLDETNLAAVLKAGAVNFCAVRAIMQSPDPEKVIRALQQIWRATV"
     gene            17859..>18190
                     /locus_tag="PKI68_03050"
     CDS             17859..>18190
                     /locus_tag="PKI68_03050"
                     /inference="COORDINATES: protein motif:HMM:NF016245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNX52703.1"
                     /translation="MKLLTLPPAMISALLLACSNVFMTFAWYAHLRDLAGRKWIIAAL
                     ISWGIALFEYLIQVPANRIGYTQLSLSQLKIMQEVIALSVFVPFSIFYMNQPLKLDYL
                     WAGLCILGA"
BASE COUNT         3731 a         4823 c         4840 g         4796 t
ORIGIN      
        1 gcgggtgtag ctcaatggta gagctccagc cttccaagct ggctgtgagg gttcgattcc
       61 cttcacccgc tccatttctc ccgacaggac tcgggctccc agattgagac ttttccctat
      121 aaaaacaagg gtttttgagg gttgtccgac tttctccgga agggactcct gaacccccga
      181 aataccccgc aaagccggtt tcgtgtgtgc agttttgtgt acacctaaga agtcgggcag
      241 agcatctacg gcctgctttt tcggaagtag ccgggcatcc gtataaacgc ccatcgtcaa
      301 tttaatatcc gaatgacgca taagttcctg agcaacgcgc ggggcggttc cgttggtttg
      361 cagcagtgtg ttgaacattt tccggagggc gtggaagtct ctgcggttgc cgcgttcgtc
      421 ttcgtacggg atgcccgccc gtttcaggtc ggatttataa accttcatgg aaggcacaga
      481 aaaaactttg tcggtctctt ttgctgaggc cgggcggaac tggcgcaacg cctcgacaaa
      541 aaactcggga atattctgtt ctgcctcttt tccgtttttg gtcgtggacg ctctggcgga
      601 aatatacggc acctctgtat cgaggtggac atcgcaccat tcgatgttgg ccagctctgc
      661 ccgccgaaat ccggtgtatg cgaccgtgat ataggccatt ttacggtttt ggtcgtcgac
      721 agacgccagc agcgtgcaga actcttccgc tgtaaagacg cgacgatcaa aagtctggcc
      781 cgcttttttc agcggctcaa ttttctcaat cggatttgtt tccgcgaagt cgtttttgat
      841 gagccatttc atgaaggcgc tgagagccga aagaaactcg ttacatgttt ttgagcaaca
      901 gggttggcgg gcccgccact gtgtgaacga aaaagcggta atatcgttga tgtgccgcca
      961 ggaacaatcc cggatcagct ttttcaggcg gtaggaggac atctggaagt gcatcggact
     1021 ccattcctgc cctttttcaa acaggtagtt ttccagatga ttgctgagcg gaagtgcgag
     1081 cgcctgcttt acgctggcgg gtacagcctg tcctacggcc tcccgctctt tttgtttcag
     1141 aagcgccaga tgtttttgag tggcgacttc tttgttcttt gttttcaggg aatggtcaaa
     1201 agtgagccct ttctcttcca actgaaaccg tctgtaccag atcccgctcg gcttccgttt
     1261 atagatatta tcgatcatgc gagagccctc ctcaatccat gatgctgcat gtattcgagc
     1321 agctcatccc agcagaccct tgttgcccgt ccgaacttga ctcgggagag ttctccgcgt
     1381 gccatcatac ggtaaattgt ttttgtcgaa attcccgaac gttgagcggc ttcctgtatt
     1441 gtgattaatg ctgtcatgtt gtgttccctt ttttcttagc cttggctttt gctttttcgg
     1501 ctttaatccg gtcttttggg taataataaa tctctttgcc ccgttcatct ttcgttgttc
     1561 cgtagatttc gagttctaag gctcttctct cttcgatggc ttcaattcgt tgagcgcggg
     1621 cttcgaggtt ttctttccac gccttcagtt cccggacaga aacatagcca taaggcgtat
     1681 cccgatgttt gaattctttt cccatccacg agaaataggt ttttgtcgtg tggaaaaaga
     1741 aggcgctcaa tcttccttcg cgcagtgctt tgtaaacggc ggcacgcgag acgggagcga
     1801 aatgtcccgc acctcccgga gaaaccattc cttccgtgca gatttgatgg actgctttaa
     1861 accacttggc atgctcttcc tgtccgcctt ctgcacggta aatccgtgtg ccgggatcag
     1921 ggtcgccgat gattgctttc agctttgcgg ggatctcttc aaatgggaat tcagttgcca
     1981 tgattgaaac ctatatttcg gtttctatta tgtcaaccag ttaaaatcag aaaaatttaa
     2041 accagacttt gttccagttc agccttcacc tttttccagt cggggaggtg actgctcagc
     2101 aggcggtaaa atgccttgtc gtgcgcggtg tgggtgatgt ggcagagctc atgggtcagt
     2161 acatagtcga tctgatgccg tgaggccttg attaacgcca gattaaggca tatccggcct
     2221 gttttgcggg agtaactgcc ccagcgcctt gtcattgagc ggatgaccaa cccgggccgg
     2281 gttttgaggg caaattgagc ggcgcaatgg tcgagccgtt ccgcaaagtg ctgccgggct
     2341 tttcctgcat accacgcatc aagcagcttt ttggtgtgaa gcctgttttg cgggaagaga
     2401 ctcaaaaccg tcagcgttcc ctgctggaga gaaacccgtt cctgttcttc cgctttgcgg
     2461 atgagcagtt tgtagtttcg gcccagatag cggaaggttt ccccgccgat gtattccttt
     2521 cgggctctgg gcttgaaccg ggagaaatac cgctgatgct tcagcaccca acggaattta
     2581 cgttgcagga agccgttgat cttttcttcc gtggcgtgca gaggcgcttt aacccgcacc
     2641 gactgatccg ggaacacggt aatcgccagc gttttccgtg attcctgcgt cagagcgtag
     2701 tcaaatgcga cctgctcgta tgtaaaccgc ttctgcatta cagccccggt tcgtctttgt
     2761 tttgcacggc cagatcccaa accagtgtga ggatggcgtc aatctcatcg aacgtcagca
     2821 gagcgccgta tttattcttc acggtatcga aaaggtagtc ttcgatttcc atccggacgc
     2881 gccgttccac ttcgatatcg ctgtcccagt ccacgatctt atgctgttcg atcagctgat
     2941 agatggtttc gaccacagag cagagttgtt cgccagtgag cggatgttcg gccagccgtt
     3001 ttttaaggtt ccggaaatac gggtgcagcg gcttgcgctc cttgagcggg gcagggatgt
     3061 cactgtcctc gtagtctgcc acttgccctt gaacctgccg cacttcaccc agcagggctt
     3121 tcacatcctc ttctttggca cttcgcagct cggcaatcag gcgggaaatt ttctcgctga
     3181 aatggcggta gaactccggg tctttactgt agttttcttt aatcgtccgc tcagtctggg
     3241 ccgcaatggc ttccgccttg gatttgtcag acaatccccg ttccgctttt tcgatgtagg
     3301 cgttgaactc tgccgattca gaaacgcaga tcggctcggc cagctcggtc acatactccg
     3361 ccgaaacata tttatccaga atgcgccgga tctggtcctc gtattttgag aaatcgaccg
     3421 tttctgcatt cttgatcttc tggattttct tcagttcgac aaaccgcttc agatccagcg
     3481 caaaccggcg gagtttctct tcatcgaact tctgggtgaa gtcataaagc atctggcagg
     3541 tggagaactg ccgggtgaac tcgtccacca gctcataaaa atgttcgcgg gtttcgatat
     3601 cgtcttccag aatccggatg tattcttccg tatcctgctt atttcggacg gaattaaaga
     3661 tgccgtgcag ttcctgataa acccgttcca gcccggcaat cttttcatcc gtattcagca
     3721 gagcccgctc aatgtcttcc ggatcataat tggaaaacag ctccatcgca tcgcgcaggt
     3781 ttttggcgtt cttggagtaa tcgatcacaa agccgttcac cttggcctct ttgcctctgt
     3841 cgccgtcaaa caggcggttc acgcgggcga tagcctgaag cagattatga tccttcagct
     3901 gttttgccag atagagaaac gtattgcgcg gggcgtcaaa gccggtcagc agcttatcca
     3961 ccacaatcag catccgcacg ccgtccgggt tctccttgaa atcatcaatc aggtcttttt
     4021 cccgtttcag cagcgacgag ccgtgcttat gcttttcctc ggccagaaac ttcaccactt
     4081 cctttttatg ttccgccagc tgatcgtctt cctccttatc ggcggactcg gaaatcacca
     4141 catcagtttg aatgcggtcc tgcagagaaa acgccttctg aaacatcacc gctgcatatt
     4201 tcgatggagc caccacctga gccttcaggc cggtatcggc gaagcgggaa tagtgttcca
     4261 gaatatccag tgcaatcgtt tcaatccgct gggaggtttc ctcgataatc tgcgacgaac
     4321 tgaacttctg ctggagatcc tttttctctc cttcgctcag atccttggtg atggtgtcat
     4381 agaactgatc cagcagcttc tgctggacgc tcagctccac atagcgcggc tggtaaatca
     4441 gcggcagcac cacgccgtcg gcttccgctt cggaaatcgt ataggcgtcg atgatgccgc
     4501 cgaatttggc cgcgctcgac ttctcttttc gcatcagcgg cgtgccggta aagccgatct
     4561 gacaggcctt cggcagaatc agattcagct cggtattagc ctggcccgcc tgcgagcggt
     4621 gtgcttcatc aatcagcaca aacacattcg gatcgttgtc cacaaaatcg cgggcagcac
     4681 ggcctgcctc gaatttgtgg attagagcgg tgatcacccg ctgatctttt tctttgatca
     4741 gttgcagcag ttcgccgctg ctggttgtct ttttgacatc cttcttgatg ttacaggcgg
     4801 cgaacgtatc ggcaatctgc cggtcgaggt cgcgccggtc ggtcaccacc agcacgcggg
     4861 gcaggacaat ttccggatca tcaatcagac actttaccag cattaccatc gtcagactct
     4921 tgccggagcc ttgcgtatgc cagatcagcc cgccgcgccg ccgtccgtac tcatcaaacg
     4981 tcttcagccg atccatcgtc tttcggatgg cgaaatactg ctgatagcgg gcgaccttct
     5041 tgatgccctt gtcgtagaga atgaaattgc gcaccaggtc cagcatccgc tccgggcgaa
     5101 ggagggaata aactccccgg tcttgctccg tcacagtgcg ttcattctgg cggtaatcgg
     5161 agcggatcaa atcctgtgca atctgcgcga ccgtttccga ttcgaccggc ttgttgacga
     5221 tggccgaaac tttcgcgtcg aaagccgccg gatcggtatc cttttccttc cagtgcgaat
     5281 aaaagcggga tggtgttagc atcgtgccgt atttcagttc gcgcccgttg gtggcaatca
     5341 gcagttgcgg gaaaatgaaa aacttcgggg tttccgtgcg gccctgatta cgcaccatct
     5401 gcgcgaccgc ctcagccacc ttcaccgacg actttttatt ctcaatcacc gcaaacggaa
     5461 tgccgttcac aaaaaccacg atgtccgggc ggcggtcgga gccttcggac agttcaaact
     5521 cggcgcagac gtggaaggta ttgttcgccg gattgcgcca atcgataaac ttcatctgcg
     5581 gggaagtgcg ccgtccgtca atcagctcgg acacgccgca accggccagc agcagagaat
     5641 agatctcctc cgacgccttg aacacgcccg cgcccatatc gatctgctgt tccgccttga
     5701 aaatctcgtc gcggatactc ttgtcggaaa tgttcgggcc gttgatcttg cgcagtgcat
     5761 cgaaagcgat ggtccgcaaa atatactgcg aagaactttc gcgcagtttg gcgacatcag
     5821 cgcgggaaat gtaggtatag cccagattca ccagctccag cagggcggga atctgtgacg
     5881 acgacgcctc gtcaaaatcc ggtagttcaa attcgggttt catagctact ccatcagatt
     5941 gtaattcggt aaaaaaatcc tgcggtttgc tcggtacatg caatagctcc acgcgacgtt
     6001 tcatttcgcg attgaaggcc ttggcatcat cacacagctc gtagttttca gaggcttgtt
     6061 tccagaagtg gtagctactc caaagttctc ggataacgat tttcttgtct tcgccatctt
     6121 tcggtttcgg ataatccgac aggaccccta agtggaaatg tgtctggatg ccttgtagat
     6181 gatcctgatg ccggtgggtt agtataaaag catcgatgta tgggcgtccc tcagaatcag
     6241 tgggtagccg gtctcttaac tcttgcgcaa catcacaatg atcagcaatg gattcttcac
     6301 caatgcatac gtcgatcagg ataactgttt tataaaagtc attcaggcgc agaagcgtca
     6361 tccctccgtt tttatcgcct acggggaaga atgtaattat cgaattagtc atgatttgct
     6421 cctttcgttg ttaaaaattc actcagcaaa cactctgcat atgcgcttgc gtaatggatc
     6481 agtatttttc tgttaaagat taacgtcagg gagaagaacg ctgtcatcaa caggctaatg
     6541 tagaatcccg ccggattgtg tatgccaaat aaaacggcgt tcaggacgga tactggtatt
     6601 gcgaaaatag aacccgcgat cagatttcgg ctgagtccgt agtgaatatt gtattgcaga
     6661 gttttgtcgc cgtccttgac ctgtttcctg atgaggttga cagcatcacg gattgtcttt
     6721 ttcgcttctt caatattcgc ctgttcttgc tctaaaggac atagccggat gccgaaatca
     6781 gtttcgattt tccccctgat tttgtttttc atctctacag agaaaaaact gtccgagaga
     6841 aggagcatcg ttgttgtcgg gaattcctta ccagctttaa aaatccgatt ttcaaacaga
     6901 tgcttggcaa tcccgcgttg cacatggatg agaaatagaa tggcgattag gctgaggctt
     6961 agattttcta cgatcaaaaa gcatcgggca ttctcaaaca gcgaaatcca cacatcctgt
     7021 ttgataaacg ctgaaagaat tatagccgga agtgctgcaa taatcgcggg gatgtatcgt
     7081 gcacgtattt catagacatt caataatttc atatttacct cctttaatcg gcttcgtaca
     7141 gggcggctgc ggggctgatt tgtcggctgg gtacagtccc gattgtttgg cgaatccatg
     7201 tgaatttatt tccggcagga tagtgtgatg aatagactgt cgcaccgtta tctttcaagc
     7261 agttaaccag tttgcgacta gggtgttttt gagagccctt ggctgagatg aaaacaactt
     7321 ttgatttgaa aagtttaacg agatcttctg acaggtttcg gcgactcccg tggtgagggg
     7381 cctgcatcca gtgaatgttt tgtaaatgcc gaaatcggtc gttgtagata atcgaactga
     7441 aagcatcaca tccggcatct ccggtgaaga gaattttgtc ttcacctagg gtcgtcgcca
     7501 agacaacgga actctcgttt tccggtgacg ttattcctcc taaagtgccg ctatcttctt
     7561 caagaatttc actgatagcc tctatcaggg ctgaggaagt gtttttccgt tccaggtaat
     7621 cattccactg tcttactttt tcgacatcgc caaaggatga gaggcactct acataaaact
     7681 ccaaagtagg gccgagaacc agaaacgggc cgatcgttgt cccagcgaag ggttcttgtg
     7741 gcgtgattcc tttacttgag gataggttat agagttcggc ctgagaggtc agcgaccgtt
     7801 ggaagcgctc caagacccac tttgcactgt ttctccccaa ctgatccttg atgccttgcc
     7861 agtctatgtg attccaggcc tgaggcatcc aaagattgtc gtacgtgaat gtatcacctt
     7921 ttattagctc aattaatccg cccgtatgat catcgtgtga gtgtgtgttt acaatgtggt
     7981 tgacgtgggt cacgcccaat cctgtcaggt gtttggctat atcgacggcg tcggatttat
     8041 tgccaccatc gattaagaca acgtcttttg tcccgccggg ataatagcgg ataacaagga
     8101 aacagtctgc gtttccaata ttcatcatga aaatttcgag tccattgtaa cttgatgcca
     8161 cgacttgatc atatgctttt tgaagctcac ctaatgtgct catcttgaca cctcgctttc
     8221 aaaattactt ttcaatcccc agcgctttga ggtgtttctg catctcaact tccagcccgg
     8281 cgagctcggt atcaacctgt ttgagttctt tgagattggc ttcgatatcg acttcctctt
     8341 cttcttcgaa ggtatcgacg tagcgagtga tgttgaggtt gtagtcgttt tctttgatct
     8401 cttccggcgt gaccgctcgg cagaatttcg ggatgtcctt ccgtgcttca accgcgtcga
     8461 gaattttctg gatgttggcc ggttccatgg tgttcatggc cttgccgggc ttgaattctt
     8521 tggaggcttc gacgaagaag acgtctttgc ggctttcgtt ctttccgccc ggttcgcggg
     8581 agcggtcgag aatcagaatg gcgaccggga tgccggtggt ctggaagagt ccggcaggca
     8641 ggccgatgac ggtatcgacg atgttttctt ccagcaggct ctggcggatg cgtccttccg
     8701 ccgcgccccg gaacaggacg ccgtgcggga cgatgaccgc gaccttgcct tctttggctt
     8761 tggccgtttc gaccatgtga gtgacaaagg catagtcgcc tttggtggcg ggcggcatgc
     8821 cgcgccagaa ccgtttgtat ttgtcgcttt cgatgttttc gcccatccat tttttcaaac
     8881 taaaaggcgg attggcgatg acgaggtcga atttcatcag cgcgtcgttt tctttgagct
     8941 gcgggttgtt gatggtgtcg ccctgttcga tgcgggcgga gtctttgccg tgcaggaaca
     9001 tgttcatgcg ggcgaggttg taggtgccca tgttgctttc ctgcccgtag agcgcgtaat
     9061 ccttggagcc ctgtttttcg atttcttcac cggccagcag cagaagtccc gccgatccgc
     9121 acgccgggtc acagatgcgc cagcccggtt gcggtttgac gagtcgcgcc agcagctggg
     9181 cgaccgattt gacggtgaaa aattcgccgg ctttttttcc ggcatcggag ccgaagcgtt
     9241 cgatcatgta catgtaggcg ttgccgatga catcgtcgcc cgcttccgtc atgtcgattt
     9301 tggcgaagtc ggtcatgagg ttgcgcagca ttttgttgcg ctgttcggtt ttgccgagaa
     9361 tggcgtcgga gttgaaatcg acggtgagga tgccttcgag tcgcgagccg ttggcgtctt
     9421 caatcttatg cagggcgacg ttgatcagtt cgcccaggtt gtcggcttcc agttttttga
     9481 agaggtcgaa gaagctggca ccttcgggaa tgatgaaccg ttcgttttta aggcgcaggg
     9541 cgatgcggtc ggggtcatcc ccgtagcgct ctttgagttt gtcgatggtg tgctggtgga
     9601 ggtcgctgat gtatttatag aagagcagca gcaggacgta gtctttgtaa acgccgccgt
     9661 ccacggtgcc gcgtgaggag tccgccgcgt tccaaagcgt tttgttgagt tcttcctgtg
     9721 tgtatttttt tacggtcatg gggtggtctc ctttttaaat cagttgctgg ttgatgatgt
     9781 tgttcgtcag ttcaattttt ctgttggtta gcgtggtgta gagcagtttt gcccgttcca
     9841 gtgcaaccag cgtttcctgt ctttctatcg ggggtaccgg catgtcgatt tgttccagat
     9901 ttttcctgct tatatgcgga atcgtcgttg tttggtctaa ccggcccagc tggcgtttcc
     9961 cttcctgaga attaaaatag agcgtaaggt attccggaag aacaggggcg tctttcttaa
    10021 cggtcagaac gaggagggcg ccggaagcga cgcaggtttc gatctggccg gtgtaggtgg
    10081 tggcagcaaa acgtccgcgg ttggaaagca gcacatctcc cgactttaga atatgttccg
    10141 gattcacttc ggacatttca acccgataga catcttcaac ttgcaggatg ccgttgttgt
    10201 cgatatcctt cggctgtatg accggaatat ttccagcggg ggaatgctta attcctgatc
    10261 ggaacgcata tcccattctt atttcgcaca tgtcttgtaa cttcatgcgc gcaatatgaa
    10321 tgtactgcat atttaagtca acttaaatat gcaatatttt tataccgcac ttctggcttt
    10381 tgtccattgg ccggatgcgt ccccgcgggc agaaaacggc cagctgaaga gttgagactc
    10441 aatcattggt ttttctcgcg gttaattttc agcgggtctc cgtcgccgat tcgatctgcc
    10501 cgaattcatc cagcgggggc ggcctcggca gcgttagaaa caaattcgcc agcggcacgt
    10561 tgaggtaata gtttgtgcgg ccaaagcggc gtttttccag aaacccggct tccgccagct
    10621 tgtccagata gcgcgcggcg gtgagtcgcg acaccgaaag ctcctgctgg aggatcgaga
    10681 ttttggtgta gggatggcgg aacaggttgt tgatcagatc ctggctgtac atccggggga
    10741 actgcttgcg gatgcggtgc ttacagtcaa gcatcatgtc ccggatggcg gtgaccagtg
    10801 cggtggtctg ccgggccgtc acggcgattc cttccagcat gaacagtacc cactcttccc
    10861 atgcgttttt tgtgcggact tcctgaagca gccggtaata gtccggcttg ttctgaataa
    10921 taaaccggct gaggtagagg atcggcaggt tcagcagccc gttggcaacc agatacagga
    10981 tgttgatgat gcgccccgtg cgcccgttgc cgtcataaaa cggatggatg ctctcgaact
    11041 gatagtggat cagcgccatc ttgatcagcg ggtcggcatc cagtcgattg gaatcgttga
    11101 tgaaggtttc cagatcgccc atcagctcca ggatcgtcgc atggtcttgc ggcggcgtgt
    11161 aaaccacctc gccggtagcc cgattgcgca gtgcagtgcc gggcagcttg cggaatccgg
    11221 cgttgttctt ttccagcgtt tcgtgaatct gcaggatctg gttgagggta atcagtctgc
    11281 tggcgcgaac agattcgaag ccgacgcgca gagcgtccgc atattgcgcc acttctttgg
    11341 cggcgggcaa gaccgattca tcggcgagtt gcgccttgaa caggtcgtcg tgcgtggtaa
    11401 cgatattctc gatttctgaa ctgtccttgg cttcctgcag ggccagcgtc tggatcagga
    11461 tgttttcgtt ggggatggat gccgcgacgc ctttgagttc ggccagatgc cggtgcgctt
    11521 cggccagctt tttcagaatg gcctttgttt caagatcaac aaatggcggc agggggttca
    11581 gtttaaacgt gctcatggtt gggcctcctg ttcagttttc tgcgaaaagg tatacatatc
    11641 cagatcaaca tgttcagaaa tcggcatttt atatacgctt cgccgacagc ttgtatcagg
    11701 atctctggat gttgaacagt tcatattcac cttaaaggcc tcatgcttta cccgctttct
    11761 tctgctttac tgtcaggctc attttggcga tcccgcctag acacagttct ccttacaaga
    11821 tgtcgccctg gcgggcgaac cgtgcgcctc ttacgagtct gcacggttgc gcccgcgagg
    11881 gacgaatcat ttttcagcag tcggaacggt ccgccctgct gctcaacggc tctctgcctc
    11941 aatcgccccg gttttcgttt cgctttgcct tgtgcgtcta cttgcagact tcaacgcagg
    12001 taagttctcg ctactctgat taccgagtcg gacagcgtga gctcgttgtc tccccagtcg
    12061 aattttgccg tctcataaat atcctttgtg agacgggaag tttcgacccc aagactcttg
    12121 cataggtacc ccgcaccatt gagccgtgcg tcgtacaagc tgaaacgggc aatcctgttc
    12181 tgcggatcat ctttatggag gcctagacgc ccccatttgg cctccagcgc atggcatgtg
    12241 ggattgttta catcccacat gccgttcgca cgctgaatct tttttggctc ccgtacagcc
    12301 gtcttgggaa gaccgccgat taagcagtga aaatgggttc gccctgtcac ttcgccctgc
    12361 tcagtacgaa gcacccagag aagctttggg aaacgcacgc cataccattt tgccaaggct
    12421 ctcgccaaag caaaccacag tttgatccgg acggcagtcg gcactcgctc cttcttaaaa
    12481 gtgagcgtgc cgaaaaactg ccagtcaatc cggctcatgg catgtatttc tggaatttcc
    12541 atttctatac cccgttgtct gagttgcggt cagaccattg aaaacaccgc ccctccgtat
    12601 ttcagaaggg gatgatttta ctgattaacc ttttttcgga accgacagga ttttcccgcg
    12661 tgttctgatc cgtccgccga agccgggcgt aagttccgta atgcccactt cgacgatctg
    12721 atttttccac tgttccggat caccgaaggt gtccttgtcg agttcgctga cgttgtaatc
    12781 aaacgaattg ctcagccggg tgtccgtgtc ctgatcgaag cagcacagcg tatgacccac
    12841 tccgtgcttg ccgttgtatt tatctactcc aattacttgg atctttatct tcatgttcta
    12901 ttccttcctt ctaaacagtc cggtattttt acacggcgca cttcaccccg gacttcatcc
    12961 gatggagcca ccgccgcagg aaacatttcc tgcttgtgtg aaattttgga gagctgctca
    13021 gcagccgggg acgccgcaat caaggcaatc tgcgaggcag gaatcctctg aattgccttc
    13081 attgcttgcg cgtctttggg agttggattg cggtctgttc ggctcattcc gagcagcgcc
    13141 tttccggaaa gaagctcttc agcgaattcc gctgatgctt ttttccccgg cgtgtcggaa
    13201 tcagccatcg cagatcgctt tttcaataag cagtacaaag cgacgaggct tggatatccc
    13261 caagaatacg ggtcggaatc tgtgccgggc gattggatgt taaaccgtct tattcctgcg
    13321 tcctgcggtt cggaaaaaga cgtgttttcg cctttgctgt aagaagagcg gttcatgcct
    13381 caccgccctt ctgtgatttc actaacgcat aaatggcgtc tttaatcggg gctgcgaggg
    13441 tgttccatga ggtcagtatg tcgtacaggc gaggctcgat ttcgagcgac gggacggcgg
    13501 ggtgttggca gttttgtgta cacccctcga cttcagcccc gtcattattg gtttgagcga
    13561 agggttcgat tcccttcacc cgctccacct tttaaggggc tgaaacttcg gttttagccc
    13621 cttatttatt ggggtttact aatacgcccg ccttctccgc ctgttttgat agcgcagaca
    13681 gttcgcgatt aaaacgggga ctcggcttcg ttttccggtc cgcctgatgt gttcgtccgg
    13741 ctctcaaaaa aacggacgat gcaaggaagg acaatgcagg cattgtcctt aaccgcagga
    13801 gaaaaacatc cgcagaattt ttgaatacgc tctaaacgaa agataagccg gaagctccgg
    13861 ccttacactt cggcagccag tctggaagct gtgcgggccc ggtttaagat ggtgttccag
    13921 tctgcgttgc ggatttcctc cggcttggcg atccacgagc cgccgactgc ggcgattaac
    13981 ggatcggcca gatagccctt gagattggtt tcattgatac cgcccagcgg aatgaatttc
    14041 ggtttcaggt atccgtaggg agcggcgata ttccggagat gggcgaggcc gccggacgtt
    14101 tcggccggga aatatttcat cagggtgcag tcgtattcca gcgcggcttc tatatcgctg
    14161 ggagtcatga ttccgggggc aaaaaacagc ccggcctgtc tggcttctgc gagaaccgaa
    14221 cggtttgtgc ccggggaaac agcaaaagcg gcgcctttgg cttttgccag acgaacctga
    14281 tccggacgga gcaccgttcc gacgccggcg agcatttccg gcacttcatt cacgatgcga
    14341 acgaggcagt ccatggcctg aggagtgcgc agggtcagct ccatggcgca aatgccgccc
    14401 tctaaaagag tccgggcaag gggaactgca ttttcaacgc agtcgatggt taatacggcg
    14461 ataacttttg catcggtaaa tttctgctgg aggttcatag cgttcctttt tgaagttgtt
    14521 ttttaatgat ccggatttgt ttaatcatcg ctgaagcggc tgcggccgct tcttaggagt
    14581 gttgcgcaag accggacgct gaaccgcatc ctgaataatc cggatgccgc tcaagaatgc
    14641 cgggagtatg cgccgttttc ataatgcctt aaaactggtc tttcctttac gcgatcagct
    14701 gaaaggaaac acgaacgaca tatgtcatga aataaaataa tgtacaaatt attcggccgg
    14761 cccgaagagt tggttccgcc cggatcctga cgtagaaacc ggatctacac acggaaccag
    14821 ttttccagcc gcaggttcgg gttcacatcg tcaggcagaa caaacggatg tccgccggag
    14881 agtttgatat atgccgcgcc ggcaatcatg gccgcgttgt cggtgcagag tctcggcgac
    14941 gaaagcttca gcggtacgcc gcatttttcg gaaagccggt tgagtttttc gcgaagaact
    15001 ttgttgagcg atacgccgcc cgcgcaggcg aacgaagcac agtcatactg ctccagcgcg
    15061 cgctcaagtc gcacggcgag tgcgtcggcg acggcttcct gatagctggc gatcagatcc
    15121 cgtttcgcgg attcgtcttt cagtgtttcg ggatgattct gcacatggta gagcagggcg
    15181 gttttgagtc cgctgaaact gaagcagagc ttgcggtcga aatcatattt ccattcgccg
    15241 ccggcgttat cgagtccgcg cgggaagcgc acggctttcg ggttgccgcc ctcggcggtc
    15301 ttctggataa tcggtccgcc gggataaccg aggccgagca gcatcgcgcc tttatccagc
    15361 gcctcgccgg ccgcatcgtc gatggtctgg ccgacgattt cgtaacagcc gggcgctttc
    15421 atgtaaacca gcttggtgtc gccgccggaa acgagcagca ccatcattgg aaacgcgccg
    15481 tcgagtacgg gcggattttc actcaggaaa accgaataga tgtggccttc gtggtgatgg
    15541 acgccggtgg gcgggatgcc gagccgctga ctgagcgccc gcgcggcgga ataaccgatc
    15601 agcagagaac tggaaaggcc ggggccgtag gttacggcga tgccgtcgag ctgttcgaac
    15661 gtgcaaccgg aaccgtgcat cgcttcttca attatgcccg gcagtttctt tacatggtcg
    15721 cgcgaggcga tttccggcac gacgccgccg tacggcgcgt gcttggcgac ttgcgaatag
    15781 atgatattgg agcgcaccgc gccgtcaacc agtacggcgg cggcggtctc atcacaggaa
    15841 gtttctattc ctaaaagaat cacttcatcc attcctgctg gatcatcata cccttgagcg
    15901 cgccgatggc gtaatcgagc tgaatatcac ggggttcctg cgcaaccgga agggctttat
    15961 cagcttctcc gtccttctgc gcctgcttca gccggatttt aaaaaggtct tcggggttca
    16021 tcacgacttt aacgtcgggc tcaataccgt gatcctgaat gacgcgttcg ctgggtgtat
    16081 aataaagggc tgtggtcagc ttgatcgccg cgccgtccgc cagcggaagg acgctttgta
    16141 cgcagccctt gccgaacgtt ttttcgccga ccagaaccgc gcgtttatga tcctgcagcg
    16201 cgccggaaac aatttccgag gcgctggcgc tgccgccgtt caccagaaga acgatcggga
    16261 agccggtgta atgtgtgctg ccggacgact taaacggttt ctgatccatt tccgcggtgc
    16321 ggcctttggt gaaaacaatc agatcgccgt gcggcagaaa aagttccgcc acacctgcgg
    16381 cggcggtcag cagtccgccg gggttcccgc gcagatcgag aaccagcgct ttcattttct
    16441 gatccgtcag ctttttcagc tctttcttca aatcttccgc cgtcggttcg ttgaactggg
    16501 tgatgcggat atagccgatg ccgtcctcaa tcagcttcgc atccttcacg ctggcgactt
    16561 caattttttc ccggatgagg tcatgttcct tcacctcatg cgtcgaaggc cggaccgcct
    16621 taagtttcac atgggttccc gcttcgccgc gcatcttctt cactgcatcc gacagcgaca
    16681 tctcccgtgt ttcctgcccg tcgattccga caatgatatc gccggaaagc agcccggcgc
    16741 ggaacgccgg cgtatcttcc atcggcgaga cgacggtcag caggccgtcc ttcatgctca
    16801 caaccagccc gacgccgctg aaacgcccgg cggtatcatc cttcatgtcg tcgtaggaat
    16861 ctttttccag aaactggctg taaggatcga gatcctgaag catgccggtc atcgcatggc
    16921 ggaccaggga atcgtaaccg gcttcattgg tatccacata actgcggtgc acctcttcga
    16981 gcaccttggt gaacagcgcg atggcgtcgt aagcgttggt ataggactcc gccgcgcgcg
    17041 gcgaggccgc gaaagcaaag agcacggaca aaaaaacagc cggtttaagt ttcatagtat
    17101 tttcctgaaa agtccggtga gcctatccga tttcgtccgg aaattggaag ctcagactgt
    17161 ggcgcgccag atctgctgaa gcgcgcggat cactttttcc ggatcggggc tctgcataat
    17221 ggcgcgaacg gcgcagaagt tgaccgcgcc ggctttcaga acggccgcca gattggtttc
    17281 gtcaagcccg ccgagcgcca cgcaggggag cggcgagttt ttaatgatcg ttccggcgcg
    17341 ctcaatgccg agcgccggat cggcgatcgc cttggtcggc gtggggaaaa ccggtccgac
    17401 cccgatataa tccggacgca gcgcccgcgc cgccagctcc tgcgcttcgc tgtgcgtgga
    17461 aagaccgaag attttaccgg gcgcattcca gagtctgcgc gcctcatcga gcggcatgtc
    17521 gccctgaccg agatgaacgc cgtcggcatc ggcggcccag gccagctcca catcgtcgtt
    17581 cataatgaaa agcgtccggg ttcccaccgt aacggcgcga accttgcggg cttctgccag
    17641 aatcgcggcg cgggacgcat ttttcatccg caactgaaca tagcgcagtc cgcagtttac
    17701 cgccgcctgc gcgcaggctt cgtatccggt gaccggatcg gtcattacca tatagagtcc
    17761 gaaattttct ttcatccaat tctccggcgg cctttttcct cttttcaaac ggattgggcg
    17821 agcctatctt ccgcgctgtt tattgcacga aaggatctat gaagctgctc acccttccgc
    17881 ccgccatgat ctccgcgctg ctgctggcct gctctaacgt gtttatgacc tttgcctggt
    17941 acgcgcactt gcgcgatctc gccgggcgca aatggatcat tgccgcgctg atcagctggg
    18001 ggatcgcgct gttcgaatac ctcattcagg ttccggccaa ccggatcggc tacacgcagc
    18061 tttcgctttc gcagctcaaa atcatgcagg aagtcatcgc cctgtcggta tttgtcccgt
    18121 tttccatttt ctacatgaac cagccgctca agctcgacta cctctgggcc ggcctctgca
    18181 tcctcggcgc
//
LOCUS       DAOPZO010000025        17823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_3327_length_17823_cov_8.181506, whole genome shotgun sequence.
ACCESSION   DAOPZO010000025 DAOPZO010000000
VERSION     DAOPZO010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 17823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17823
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3327_length_17823_cov_8.181506"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="PKI68_03055"
     CDS             complement(<1..1022)
                     /locus_tag="PKI68_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52704.1"
                     /translation="MKKIETMITAFRDGFQSVYGARVFTKDFLPAVEATKAAGISHFE
                     AGGGARFQAPFFYCNENAFDSMDAFRKVAGPDANLQTLARGVNVVALDSQSSDIIKLH
                     AQLFKKHGMTTIRNFDALNDVNNLIYSGQCIHDAGLKHEVVVSMMALPPGCEGAHDAA
                     FYEKTLRAILDAGIPYDSVCFKDASGTSTPATIYDTIKRARKMLPAGTKIAFHSHDTA
                     GTCVACYMAAINAGADQVDLSMAPASGGTCQPDVISMWHALRGSEFSLNVDIDKMREA
                     EEVFKDCMKDYFLPPEAKEVEPLIPFSPMPGGALTANTQMMRDNGIMNRYPEVIKAMG
                     EVVRLGG"
     gene            1189..1902
                     /locus_tag="PKI68_03060"
     CDS             1189..1902
                     /locus_tag="PKI68_03060"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNX52705.1"
                     /translation="MKFYLQWYARLPSTNSFLKERLAVDRDLPSGTVAAAREQTQGRG
                     RREREWLSGSGENLTFSLLLRGAYSLRQLPSASMAAALAVAELLEAEGLKPVLKWPND
                     VLVNGKKICGILSEGSSGCVIVGIGLNVNMKNAEHIDQPATSILVETGNRRDVDELLG
                     KLLKHLAVRLDEWAQGGFTGIRKGWEAKVQNLGKSVTVRDGGLFRSGILAGFGGDGEL
                     LLRDEAGAVNSVWAGDLSI"
     gene            1899..3953
                     /gene="uvrB"
                     /locus_tag="PKI68_03065"
     CDS             1899..3953
                     /gene="uvrB"
                     /locus_tag="PKI68_03065"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNX52706.1"
                     /translation="MSASFQLVSNYSPTGDQPEAIAALCRGLERGQRFQTLEGVTGSG
                     KTFTMANVIARQGRPALVISHNKTLAAQLYAELKSFFPHNAVEFFISYYDYYQPEAYI
                     PQTDTFIEKDAAINEEIERLRLAATDSLLNREDVIIVASVSCIYGLGSPEDYRDMVLS
                     AHLGDACGRDEVLKKLVAIQYERNDYEPKSGTFRVRGDTVDIFPSYAQHGIRVAFFGN
                     EIESIRRIDPLTSVVEEELERITISPAKHFVMAADKLEPALARIKAELDERVKWFEDH
                     DKLIEAQRIRMRTMYDIEMLKEIGYCGGIENYSRHLAGRVAGDRPACLIDYFPKDFLT
                     VIDESHVTLPQIRGMFNGDRARKTTLVEHGFRLPSALDNRPLEFNEFLDVTGPILFTT
                     ATPGPFELEHGGKTIEQVIRPTGIIDPPVEVRPLKGQIDNVMEEIRARAERGERTLVT
                     TLTKRTAEDLTDYLKKVGLRVEYLHSEVDAIERVEILRGLRKAEFDCLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSETALMQTAGRAARHIDGKVIMYADNITGSMCRMMDIT
                     AHRREKQIAYNTEHGITPQSIRKEIQESLRHLKQDAEELEENIVRETGEDYDINQTLR
                     EIEQEMLEASGKLEFERAALLRDQLYELKASAAPPGSRTTGTPGKMLYAGRKMRGRKQ
                     GR"
     gene            4026..4181
                     /locus_tag="PKI68_03070"
     CDS             4026..4181
                     /locus_tag="PKI68_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HNX52707.1"
                     /translation="MTMHSSLKSASKIAIRRNVLKRFERVDQLKAEGKWKDGDRGFGL
                     VKTKPTE"
     assembly_gap    4235..4334
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4335..5057
                     /locus_tag="PKI68_03075"
     CDS             <4335..5057
                     /locus_tag="PKI68_03075"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNX52708.1"
                     /translation="MIRLQKYLAECGVASRRACEKLITDGLVTVDGVTVTELGTKVDP
                     AVQKVTCDGKPVQLDQKIWIMMNKPVGVICTSDDPEGRERAIDLLPEIQGRFYTVGRL
                     DVMSEGLLLITNDGELAHRLMHPRHHIEKKYQVWIDRELTAEEIDRMLKGLNCRGEEL
                     RALRVEPLRRMIASQPGYTLTLGEGRNRHIRRMMEVLGCKVIRLVRVSVGPLRLEKLR
                     SGEHRHLEPWEVEKLKKAVDLA"
     gene            complement(5089..5292)
                     /locus_tag="PKI68_03080"
     CDS             complement(5089..5292)
                     /locus_tag="PKI68_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52709.1"
                     /translation="MQTNLHHTMQWMHHEKALVGHWCSVHMVHDYHFWVYLLFAVIII
                     ALIAMALSGGGTVLDHELRYYAF"
     gene            5630..8305
                     /locus_tag="PKI68_03085"
     CDS             5630..8305
                     /locus_tag="PKI68_03085"
                     /inference="COORDINATES: protein motif:HMM:NF014348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase family 8 super-sandwich
                     domain-containing protein"
                     /protein_id="HNX52710.1"
                     /translation="MKQWLLKLGIFVLIAARAHGAGWNVEVQKERYQNLLLFKESDYQ
                     TVPLAGTILSKGLLPEFKSAVEAAKNFSYELQGVPAYYDYKVFAESTLSAEQQAREKS
                     NRKLSALFTHIIPVLAYAYQTPGANPYYHDQEVLQLYIKGLEYCYSRGLTEEAWLSDH
                     AGKASSIGLKQGLSRLAGDFASESLNFGGFSQSVFLMREPLAQAGLLGKYRSVIRNLA
                     VNCGPMYPAFYKAARKDAGIGFGNSISDDEAYFLNADGMRLFTDYFWPYFLLIEDPAE
                     REQMTGILKKVIAVNIKKQCGAQDTIKPDGVGFHHLAPYVGAYSPYTFETFAQLLYLL
                     SGTDFYTQENYTAVKEALEGFRVMVQKYTVTPALTGRLVQGQQGAASVAITKAMCMLA
                     SPGGFSDVDMQRRFLEFFDADYFFSNEQLTKYYRGQRGVTIRGLGIYRLIDEVRKSGV
                     APAPTPSGVWIKPYAAAAFHRRDNWLVTARGFSRYFWDYEGPLNKQENSFGQNWSYGL
                     LQVFSAGTPVSLTGSGCDLNQGWDWYHVPGTTASHYPIVKRSEKMVIQARREAGVKQK
                     DTHRNYSSKTFVGGVSLGEHGLFVDDLEALPFTSQTDLRARKSYFFVGDKVLALGTHI
                     SGGTAQDETHTTLFQTKLTNAKSATLLNGKTLTGLETLEHLPAGKPAAMSDSVGNSYY
                     LADSSAELILSRTNQQSMTTGYQPTSGNYIQAWINHGIKPKQQHYQYVVIPADKDGLK
                     LKQLADNPAAYCQVLQSDRMHLVYFPEQQITAYAFYELVETPKPQLVKNSSLNATVMT
                     CRQGAEVTLAASVPDLGWDANVEELQIGGLGYAGLQYERQEAKLHKLQLTLRGKWKLA
                     KENPKASLNHKANETILTLSCKDGLSEQVRLIPAE"
     gene            complement(8391..11279)
                     /locus_tag="PKI68_03090"
     CDS             complement(8391..11279)
                     /locus_tag="PKI68_03090"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNX52711.1"
                     /translation="MNNFKLIREQFVREFNGTVKEYRHEPTGAEYISVENGEDNKVFG
                     ITFRTPPADSTGVAHILEHTVLCGSRNYPLKDPFVQLLKGSLQTFLNAMTYPDKTVYP
                     VASQNEQDFYNLVDVYLDAVFFPRITPDFFRQEGWHYELESPEAPLTCKGVVYNEMKG
                     AYSSPDSILLERSQQALFPDITYGLDSGGNPAEIPTLTYEAFCQFHKTHYHPSNARIF
                     FYGNDNPEHRLKILSEYLDGFQRLENPPDSSVPLQPPFEKPVYVEEKYAVSEEGDEHA
                     FITVNWAMPDNALHFALTVLDQILTGTSGSPLRKAMIESGLGEDLAGFGLETSLRQPA
                     WSIGMKGVEIENLEKVEALIFRTLEKLAHDGLDRGDIEAAMNSFEFDLRENNHGSFPQ
                     GLSIMLNMLETWLYDRDPVGLAAYEQPLAELKTNIAANPRYFEELIETLLLKNPHRAI
                     VRLIPDKDKAARDEAEEKARLQAVRNGMSPEALADTVRIAARLLEMQKTPDTPEAVKT
                     LPLLKRSDLDRKIRTVPRELEQRDNADILFHDLFTGGIVYVDIGFNLHVLSADDLPWV
                     SLLGSLLLEMGTRKEDFASLSQRIAQKTGGLYAAPLLAALENSTGSAAYLFLRGKCMS
                     GQTGDLFDILHDILFEPAFNNRKRFKEVLLEQKAEMEGSLVPSGHSAVLSRLKAHYHE
                     AHRAAEAMSGIDALLFHREMQKKMKEDWPGTVARLEALLQKLLAGGLTVNITAENKDR
                     APVFQTLEKFLRHFDQTGKASVTDWTFREQPPASEALLIPSRVNYVGRAINLFDNGYP
                     MDGSSLVITKYLRTAWLWEKIRVQGGAYGAMCTFDPNCGLLAFASYRDPNLSATLDAY
                     GQTAGFLKNLQLDDDELTRALIGAIGGIDAYLLPDARGYSDMVRWLMGQTDETRQQRR
                     DEVLSTTAADFKAFAEFLSMDKAVTSVLSSPEVIKKSGVAFQTVLKVL"
     gene            complement(11325..12533)
                     /locus_tag="PKI68_03095"
     CDS             complement(11325..12533)
                     /locus_tag="PKI68_03095"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNX52712.1"
                     /translation="MRDCLPMQATISKRNLFAGVFAVLTVGTMALFFNIYEPWRPVGP
                     ELIPDGSFSTPAATNVWSGWNEWTKLVPAGGFGGSPGVVLTASSNQHGILRFTAHDLT
                     NIPAFRVSLRAAAHGVVRGKEGYHIPRAVFFYNDAQAKSLFKLHHGVMDIPKDRGWHH
                     YKDFFPVPEGAVDARLQIQNLGIAGTMQIDDVCVIPVCERPSAPWWKLFFGVLWTTAF
                     GLCLFALKPWMRRHGFLIMLTLILIMTGIVLPGKFLDSSIEKTVHTAKELIHKTAPPP
                     PVQAVKTSPAPSAPSKPKGETPFIALAGTLVDQVHTTGHFILFSWLALLTSLSWIAAP
                     PALRRITAVFAGLAFFAAATETLQFITIDRAAALSDLCVDIVGMAGAVALVFVLRGIQ
                     YAVRLLINRD"
     gene            12580..13212
                     /locus_tag="PKI68_03100"
     CDS             12580..13212
                     /locus_tag="PKI68_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNX52713.1"
                     /translation="MKTYLDEYKAFIFDLDGTLINSEKYHADGFAYAVETLSGYRLTP
                     AERREFFEGHTHLFTPVLAERHGLTLDPNEVLARKRVHVDKHFKVEVLPGAEAFIEKW
                     RGQMRLALASNSPRPFVKQALVGAGLIDVFESVITADDVTHRKPDPEMFILTLENLRL
                     KPDDVIVFEDSPAGVAAAQAAGCPVVMIENGAGRSVEGVPRYTWKELKQF"
     gene            13209..14288
                     /gene="gcvT"
                     /locus_tag="PKI68_03105"
     CDS             13209..14288
                     /gene="gcvT"
                     /locus_tag="PKI68_03105"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNX52714.1"
                     /translation="MKTTPLHGEHVALGARMAEFGGWDMPIQYEGILAEHEHTRTQTG
                     LFDICHMGELELTGPSAAADLERLLTMNVSTLAAGQCRYGFLLNEQGGVIDDLTCYRL
                     GEERYMLVVNASTAEGDAAWVRQHLSPQTGFADLSDGIAKLDVQGPTSRAELEKVFGQ
                     KLPDLGYFRAEYVQCLGTEMLVSRTGYTGDWGYELYFPAAEAVRIWRALLANGNIKPV
                     GLGARDTLRLEMGYSLYGHELSLDRTPVATSRGAFIRKEGGFIGEAAVRHDLAAPREF
                     LVALEFDSKRAARAHDKVFSGGAEIGQVTSGSVSPSLGKAVALAFVAAGCAAVGKVLD
                     VEIRGKLFSAKVVELPFYKNGTARG"
     gene            14288..14941
                     /locus_tag="PKI68_03110"
     CDS             14288..14941
                     /locus_tag="PKI68_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNX52715.1"
                     /translation="MHRQLLVFDLDGTLADSRADLATAVNLMRAHYSLPPLSLDTIES
                     YVGNGVRQLVARSLQDTGVDVAEALPLYKQFYTEHMLDATALYPGVAQGLRRLAESGH
                     LLAVLSNKPGDPSRTILHHLGAGPLFAWILGGGDLPNLKPAPDGLLALVAATGMPPQK
                     VWMIGDHHTDLEAARNAGVKSGFVTYGIGNPGGFRADQVWNSFEELVDFFANKRSVL"
     gene            14938..>15269
                     /gene="queF"
                     /locus_tag="PKI68_03115"
     CDS             14938..>15269
                     /gene="queF"
                     /locus_tag="PKI68_03115"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNX52716.1"
                     /translation="MSNTEGLKLLKKGEMNYPKKPDASILETFKNSNPQRDYWITFET
                     SEFTSLCPITGQPDFATIKIEYIPNELCVESKSLKLFLFSFRNEGSFYEDVTNRIFTD
                     LTNVCKPRR"
     assembly_gap    15270..15369
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15370..15954)
                     /gene="queC"
                     /locus_tag="PKI68_03120"
     CDS             complement(<15370..15954)
                     /gene="queC"
                     /locus_tag="PKI68_03120"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNX52717.1"
                     /translation="MKKKAVVLLSGGLDSATALAIAKSQEFDVYALSFRYGQRHAVEL
                     ECAKQTAEALGVCEHRTVTIDLGAFGGSALTDPTLAVPHAPGRPDEIPITYVPARNTV
                     FLSYALAWAEVLGSFDIFIGVNALDYSGYPDCRPEFIAAYEKMANLATKAGVTGSEKL
                     TVHTPLINLTKAQIIQKGVELGVDYGKTSSCYDPA"
     gene            complement(15951..16724)
                     /locus_tag="PKI68_03125"
     CDS             complement(15951..16724)
                     /locus_tag="PKI68_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNX52718.1"
                     /translation="MSKTDKITAFVSEIFSSVQGEGVNIGRRQLFVRFCECHRNCLYC
                     DTPVARTAAAAIEREPGSGQFDSIPNPLSVDQLLELLAGLSRPEFAHDDLFITGGEPL
                     LQAEFLAAFLPAARKQLDLPVHLETSGDLPEPFEQVVEWVDHVLMDIKLPSVTGEPET
                     WAMHRDFLEIIEREETGATIKLVVSADTADGDLAQAAGIIRDSGSPAAVVLQPMTAAS
                     KTDRVPTAKQVLGWQSQMAVALGRSVRVIPQCHKMMGLL"
     gene            16794..>17823
                     /gene="queA"
                     /locus_tag="PKI68_03130"
     CDS             16794..>17823
                     /gene="queA"
                     /locus_tag="PKI68_03130"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNX52719.1"
                     /translation="MKVDLFDFELPQHFIADRPASPRDSARLLDLTGAGFANRIVRDL
                     PELLQPGDLLIGNNTKVIPARLDGVRGEVKIEVTLHMRASDNEWFAFAKPGKRLKKDS
                     LIDFADDFFAEVVEKRDGGEILLRFNRRGAALFECLEKYGRMPLPPYIQRKRDDERTA
                     SDRESYQTVFARHEGAVAAPTAGLHFTPELFQALSKRGVGFDTVTLHVGAGTFLPVKV
                     DNTEDHKMHAEWGTVSQETVDRINATRLAGGRIVAIGTTSLRLLESAATAPGELQPFA
                     GETSIFITPGYQFKMVDLLMTNFHLPRSTLFMLVSAFAGLDRMHAAYEHAKNEGYRFY
                     SYGDACLLK"
BASE COUNT         3683 a         4971 c         5418 g         3551 t
ORIGIN      
        1 ccgccgaggc gaaccacttc gcccatcgct ttgatcactt cggggtagcg gttcatgatg
       61 ccgttgtcgc gcatcatctg cgtatttgcc gtcagcgcgc cgcccggcat gggcgagaaa
      121 ggaatcagcg gctcgacttc cttggcttcc ggcggcagga agtagtcctt catgcagtcc
      181 ttgaaaacct cttccgcttc gcgcattttg tcgatatcga cgttcagcga aaattccgag
      241 ccgcgcagcg cgtgccacat gctgatgaca tccggctggc aggtgccgcc ggaggccggc
      301 gccatcgaaa ggtcaacctg gtcggcgccc gcattgatcg cggccatata gcaggccacg
      361 caggttccgg cggtgtcgtg cgagtggaag gcgattttcg tgccggccgg aagcatcttg
      421 cgggcgcgct tgatggtgtc gtaaatcgtg gccggggtcg aggtgccgga ggcatcctta
      481 aagcagacgc tgtcgtacgg aatccccgcg tccagaatcg cgcgcagcgt tttttcgtag
      541 aaggcggcat cgtgcgcgcc ttcgcagccg ggcggcaggg ccatcatcga aacgacgact
      601 tcatgtttga gtccggcgtc gtggatgcac tgtccggagt agatcaggtt gttgacgtcg
      661 ttcagcgcat caaaattgcg gatggtggtc atgccgtgct ttttaaagag ctgagcatgc
      721 agtttgatga tgtcgctcga ctgcgagtcc agcgcgacga cattcacgcc gcgcgccagc
      781 gtctgaagat tggcgtccgg accggccacc ttgcggaagg cgtccatcga gtcaaaggcg
      841 ttttcgttgc agtaaaagaa cggagcctgg aagcgcgcgc cgccgccggc ttcgaagtgg
      901 ctgatgcctg ccgccttcgt ggcttcaacc gccggcagga aatctttggt gaatacgcgg
      961 gcgccgtaaa ccgactggaa gccgtcgcgg aaggcggtga tcatagtttc tatttttttc
     1021 atgttagagc actcctgtta ttaaatctca tagccgccga tccagggaat tgcggtcaga
     1081 ataaattatc cgtccgtaaa tttcctttgt ttcggatttg aaaaaaatta gcctgctttg
     1141 tacggaaact aaggatccaa agccagaaaa accggcacaa aaatttatat gaaattctat
     1201 ctccagtggt atgcacggct tccgtccacc aactccttcc tcaaggaacg gctggctgtg
     1261 gaccgcgacc tgccgtccgg cacggtcgcg gcggcccgcg aacagacgca gggccggggc
     1321 cggcgcgagc gcgaatggct ctccggttcg ggcgaaaatc tcaccttttc ccttcttttg
     1381 cgcggggcct acagcttgcg ccagctgccg tccgcctcca tggccgccgc cctcgcggtt
     1441 gccgagctgc tggaagccga aggccttaaa ccggttctga agtggccgaa cgacgtgctg
     1501 gtgaacggta aaaaaatctg cggcattctg tcggaaggca gttccggctg cgtgattgtc
     1561 ggcatcgggc tgaacgtcaa catgaagaat gcggagcaca ttgatcagcc tgcgacttcg
     1621 attctggttg aaacagggaa ccgccgtgac gtggatgaac tgcttggaaa gctgttgaag
     1681 catctcgccg tgcggctcga cgaatgggcg cagggcggat ttaccggtat ccggaaaggc
     1741 tgggaagcca aggttcagaa tctgggtaag tccgtcaccg tccgcgacgg cgggcttttc
     1801 cgctccggaa ttctggcggg atttggcgga gacggggaac tgctcttgcg cgatgaagcc
     1861 ggcgctgtaa actcggtctg ggcgggtgac ttatccatat gagcgcttcc ttccagcttg
     1921 tttcgaacta ttcgccgacc ggcgaccagc cggaagcgat tgccgcgctc tgccgcgggc
     1981 tggagcgcgg gcagcggttt cagacgctgg agggggtaac cggctcgggc aaaaccttca
     2041 ccatggccaa cgtcattgcc cggcaggggc ggcccgcgct ggtgatttcg cataacaaga
     2101 cgctggcggc gcagctttac gccgagctga aatccttctt cccgcacaac gccgtcgagt
     2161 ttttcatcag ctattacgat tactaccagc ccgaagccta cattccgcag acggacacgt
     2221 tcatcgaaaa agacgccgcc atcaacgagg agatcgagcg gctgcggctg gcggcgaccg
     2281 acagcctgct gaaccgcgaa gatgtgatta ttgtcgcgtc ggtttcctgc atctacggcc
     2341 tcggttcgcc ggaggattac cgcgacatgg tgctctcggc gcatctaggc gacgcctgcg
     2401 ggcgcgacga ggtgctgaaa aaactggtcg ccattcagta cgaacgcaac gactacgaac
     2461 cgaaatccgg aaccttccgc gtgcgcggcg acacggtgga cattttcccg tcctacgcgc
     2521 agcacggcat ccgcgtcgcc tttttcggca acgagatcga aagcatccgc cggattgatc
     2581 cgctgacctc tgtcgtcgaa gaagagctgg aacggatcac catttcgccc gccaagcatt
     2641 ttgtcatggc ggcggacaag ctcgaaccgg cgctggcgcg gatcaaagcc gagctcgacg
     2701 aacgggtgaa gtggtttgaa gatcacgaca agctgatcga agcgcagcgc atccgcatgc
     2761 ggacgatgta cgacatcgaa atgctcaagg agatcggcta ctgcggcggc atcgaaaact
     2821 attcccgcca tctcgcgggc cgcgtggccg gcgaccggcc cgcctgtttg atcgactatt
     2881 ttccgaagga tttcctgacg gtcatcgacg agtcgcatgt gacgctgccg cagatccgcg
     2941 gcatgtttaa cggcgaccgc gcccggaaaa ccacgctggt ggagcacggt ttccgcctgc
     3001 cgtcggcgct cgacaaccgt ccgctcgaat tcaacgagtt tctcgacgtc accggcccga
     3061 tcctgttcac gaccgctacg cccggtccgt tcgagctgga gcacggcgga aaaaccattg
     3121 agcaggtcat tcgcccgacc ggcatcatcg atccgccggt tgaagtgcgt ccgctcaagg
     3181 ggcagattga caatgtaatg gaggaaatcc gcgcgcgcgc tgagcgcggc gagcggacgc
     3241 tggtgacgac gctgacgaaa cgtacggcgg aagatttgac cgactatctg aaaaaggtcg
     3301 gcctgcgcgt cgaatatctt cattccgaag tcgatgccat cgagcgcgtc gaaattctgc
     3361 gcggactgcg caaggcggag ttcgactgcc tggtcggcat caacctgctg cgcgaagggc
     3421 tcgacctgcc ggaagtgtcg ctggtcgcga tcctggatgc cgataaagaa gggttcctgc
     3481 gctccgaaac cgcgctgatg cagacagccg gccgcgccgc ccggcatatt gacggtaagg
     3541 tcattatgta cgccgataat attaccggtt ccatgtgccg gatgatggat attaccgccc
     3601 atcgccggga aaaacagatt gcctataaca cggaacatgg cattacgccg cagtcgatcc
     3661 gcaaagagat tcaggaaagc ctccggcatc tcaaacagga tgcggaagag ctggaagaaa
     3721 acatcgtgcg cgaaaccggc gaagactatg acatcaacca gaccctgcgc gaaatcgagc
     3781 aggaaatgct cgaagcatcc ggcaagctcg aattcgagcg cgccgcgctt ctccgcgacc
     3841 agctttacga actgaaggcc tcggcggcac cgcccggctc tcggacgacc ggaacccctg
     3901 gcaaaatgct ttatgcgggg cgcaaaatgc gggggcggaa acaaggcaga tgagggtttg
     3961 acaaccggtt cagaaaaggt attctgtgcc gctcgtttac aaaaagattt ttgaggagaa
     4021 gaaccatgac aatgcattcc agtttgaaat cggccagcaa aatcgcgatc cgccgcaacg
     4081 ttctcaagcg tttcgagcgc gtggaccagc tgaaagcaga aggcaagtgg aaagacggcg
     4141 accgtggctt cggtctggtt aaaaccaaac cgactgaata agccagttga ccttattcca
     4201 gtaaggcccc ggtattgccg gggctttttt tgtgnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnatgatc agactgcaga aatatctggc ggagtgcggg gtggcgagcc
     4381 gccgcgcctg cgaaaaactc atcaccgacg gtctcgtgac cgttgacggg gttaccgtga
     4441 cggagctcgg caccaaggtg gatccggcgg tccaaaaggt tacctgcgac ggcaagccgg
     4501 ttcagctgga tcagaaaatc tggatcatga tgaacaaacc ggtcggcgta atctgtacgt
     4561 cggacgatcc ggaaggccgc gagcgggcga ttgatctgct gcccgaaatt cagggccgct
     4621 tttacaccgt tggccgcctc gacgtgatga gcgaaggatt gctgctgatt accaacgacg
     4681 gcgaactggc gcaccgttta atgcatccgc gccaccatat tgagaagaaa tatcaggttt
     4741 ggatcgaccg cgaactgaca gcggaagaaa tcgaccggat gctaaaaggg ctcaactgcc
     4801 gtggcgaaga attgcgcgcc ctccgggtcg agccgctgcg ccgtatgatc gccagccagc
     4861 ccggctacac actcacgctg ggggaagggc gcaaccgcca catccggcgc atgatggaag
     4921 tgcttggttg caaagtcatc cgccttgtcc gcgtctcggt cggcccgctc cgcctcgaaa
     4981 agctgcgcag cggcgaacac cggcaccttg agccgtggga agtcgaaaag ctgaaaaagg
     5041 cagtcgacct cgcctgacca aaaacgacgc tgttctcagc gccgggatct agaacgcata
     5101 atagcggagt tcatggtcga gaaccgtccc gccgccgctc aatgccatcg caatcagtgc
     5161 gatgataatt acggcaaaca gcagatagac ccagaagtga tagtcatgca ccatatgcac
     5221 actgcaccag tggccgacta acgctttttc atgatgcatc cactgcattg tatgatgaag
     5281 attcgtttgc atgctgcacc tcctctcatc acccagttag acgccggagc tatccgggcg
     5341 ttgaaacggt cagcggactt tttgtgctgg atttgaaagt ggctgaaaaa tcccgtcgta
     5401 ttccaatcag gattcatcag gttcctttcc cgtgcaggcc ttcctggccg ctacgtatag
     5461 aaggccggat gtttacggta taactgaaaa tctgcgcaaa cggatggctg ttcggcggag
     5521 gcgttgtcta cagtaaaatt tcgccgatag cgttcgacga acaaggctgt gctcttggta
     5581 ggcaccgggt gaggggtggg gttatcatcg caacaaggga aataaaaaca tgaagcaatg
     5641 gctgttgaag cttgggatat tcgtattaat tgctgcccgg gcgcatggcg ctggatggaa
     5701 tgtcgaagtg cagaaggaac gctaccaaaa ccttctgttg ttcaaggaaa gcgactacca
     5761 gacggtgcca ttggccggta cgatcctcag caaagggctt ttgccggagt ttaagtcggc
     5821 ggtcgaggcg gcgaaaaact tctcctatga actgcaaggc gtacccgcct attatgatta
     5881 taaggtgttt gccgagagta cgctttctgc ggaacagcag gcgcgggaga aatcgaaccg
     5941 gaagctgtcg gcgttattta cgcatatcat accggtgctg gcctatgcct accaaacccc
     6001 gggggcaaat ccttattacc atgatcaaga ggttctccag ctctatatca aaggcctcga
     6061 atactgctac agccgggggc tgaccgaaga ggcttggctt tcggatcatg cgggcaaagc
     6121 cagttccatt gggcttaagc aggggttgtc gcgtcttgcg ggtgatttcg cctccgaatc
     6181 gcttaatttc ggcggtttta gccaatcggt ttttctgatg cgcgagccgc tggcccaggc
     6241 cgggctgctg ggtaaatacc ggtcggtgat acgcaatctg gcggtcaatt gcgggccgat
     6301 gtatccggcg ttctataaag cggcccgcaa ggatgcagga atcggcttcg gtaattccat
     6361 ctcggacgat gaagcctatt ttctgaatgc ggacggcatg cgtctgttca cagattattt
     6421 ctggccctat ttcctgctga tcgaagaccc ggccgagcgg gagcagatga ccggtattct
     6481 gaaaaaagtg attgcggtga atatcaagaa gcagtgcggc gcgcaggata caatcaaacc
     6541 tgacggcgtc gggttccatc acttagcgcc ctatgtcgga gcctacagcc catacacgtt
     6601 tgaaacgttt gcccagttgc tctatctcct ttcgggcacc gatttttata cacaggaaaa
     6661 ctataccgcc gtcaaggagg ccctggaagg gttccgggtc atggtgcaga agtatacggt
     6721 gacgccggcg ctgaccggtc ggctggtcca aggccagcaa ggcgcggctt ccgtcgccat
     6781 caccaaggcg atgtgtatgc tggccagccc cggcggcttc agcgatgtcg acatgcagcg
     6841 ccggttcctc gagtttttcg acgcggatta tttcttcagc aacgaacagt tgacgaaata
     6901 ctatagaggc cagcgtggtg tgacgatccg cgggctgggg atttaccgcc tgatcgacga
     6961 ggtgcgcaag tcgggcgtgg ctccggctcc gactccgtcc ggagtctgga tcaaacccta
     7021 tgcggccgcc gcctttcatc ggcgcgacaa ctggctggtg acggcgcggg gattcagccg
     7081 gtatttctgg gactacgaag ggcctctcaa caagcaggag aacagcttcg gccaaaactg
     7141 gtcgtacggc ctgctgcagg tcttcagcgc cggcactccc gtgagcttaa ccggttccgg
     7201 ttgcgatttg aaccaagggt gggattggta tcacgtgccg ggaaccaccg ccagccacta
     7261 tcccatcgtg aagcgctccg aaaagatggt gatccaggcc cgcagggaag cgggtgtaaa
     7321 acagaaagac acgcatcgga attatagcag taaaaccttc gtgggcggcg tcagtctcgg
     7381 cgagcacgga ctgtttgtgg acgatctcga agccctgccg ttcacctcgc agaccgacct
     7441 gcgcgcccgt aaaagctatt tctttgtcgg tgacaaagtg ctggcgcttg ggacgcatat
     7501 ttccggcggc accgcgcagg atgaaaccca cacgacgttg ttccagacca agctgacgaa
     7561 tgcgaaaagc gcgaccctgc tgaacggaaa aacgctgacc ggattggaaa ccttggaaca
     7621 cctgcctgcc ggcaaacccg ccgcgatgag cgacagcgtg ggcaacagct attatctggc
     7681 cgacagctcc gccgaattga tcctcagccg aacaaaccag caaagcatga cgacgggata
     7741 tcagccgacg tcaggcaact atatacaggc ctggatcaac cacggcatca aacccaagca
     7801 acagcactac caatacgtcg tgattccggc ggataaagac ggccttaagc taaagcagct
     7861 ggcggataac ccggccgcgt attgccaagt gctgcaaagc gaccggatgc acctcgtgta
     7921 tttcccggaa cagcaaatca ccgcctatgc cttctacgaa ctggttgaaa cgcccaaacc
     7981 gcagctggtg aagaactcca gcttgaatgc gaccgtgatg acttgccgcc agggcgcaga
     8041 agtgacgctg gccgcaagcg tcccggacct cgggtgggat gccaacgtgg aagaactgca
     8101 aatcggcgga ctggggtatg caggcctgca atatgagcga caggaagcaa agctgcacaa
     8161 actgcaactc accctgcgcg ggaagtggaa gctggcgaag gagaatccca aagccagcct
     8221 gaatcataaa gccaacgaaa cgatcctgac gctgagctgt aaggacggcc tgtcggaaca
     8281 ggtccgcttg atacccgccg aataagcgca atggatttgt ccgttttgga gcggcagggt
     8341 tatggccaga gacttcccat aatcctgccg cagcccgctg tgcggtcagc ttataaaact
     8401 ttcaaaacgg tttggaatgc aaccccgctc tttttgatca cttcgggcga gctgaggacg
     8461 gaggtcaccg ccttgtccat gctgaggaat tctgcgaagg ccttgaaatc tgcggcggtg
     8521 gtggaaagca cttcatcgcg gcgctgctgc cgggtttcgt cggtttgtcc catcagccag
     8581 cggaccatgt cgctatagcc ccgggcgtcg ggcagcaggt aggcgtcgat gccgccgatg
     8641 gcgccgatga gcgcgcgggt gagttcgtcg tcgtcgagct gaaggttttt caggaagccg
     8701 gcggtctggc cgtaggcgtc gagcgtggcg ctgaggttcg ggtcgcggta ggaggcgaag
     8761 gcgaggagcc cgcagttcgg gtcgaaggtg cacatcgcgc cgtaggctcc gccctgcaca
     8821 cggatttttt cccaaagcca ggcggtgcgc aggtatttgg tgatgacgag cgaggagccg
     8881 tccattggat agccgttgtc gaacaggttg atggcgcgtc cgacatagtt gacgcgcgac
     8941 ggaatcagca gggcttccga agccggcggc tgctcgcgga aggtccagtc cgtgacggaa
     9001 gctttgccgg tttggtcgaa atgccgcagg aatttttcga gggtttggaa caccggcgcg
     9061 cggtctttat tttcggcggt gatgttgacg gtgaggccgc ccgcaaggag tttttgcaga
     9121 agcgcttcca gccgagcgac ggtgccgggc cagtcttctt tcattttttt ctgcatttcc
     9181 cggtgaaaca gcagcgcgtc gatgccgctc atggcttcgg cggcgcggtg ggcttcgtgg
     9241 tagtgcgctt tgagccgcga gaggacggcg gagtgtccgc tgggcaccag cgaaccttcc
     9301 atctcggctt tctgttcgag caggacttcc ttgaagcgtt tgcggttgtt gaacgcgggc
     9361 tcgaaaagga tgtcgtgcag gatgtcgaag aggtcgccgg tctggccgct catgcatttt
     9421 ccgcgcagga aaaggtaggc ggcgctgccg gttgaatttt ccagcgcggc aaggagcggc
     9481 gcggcgtaga gtccgccggt cttctgcgcg atgcgctgtg agagcgaggc aaagtcctct
     9541 ttccgggtgc ccatttcgag cagcaggctg ccgaggagcg aaacccacgg caggtcgtcg
     9601 gcgctgagga cgtggaggtt gaagccgatg tcgacataga cgatgccgcc ggtgaaaagg
     9661 tcgtggaaaa gaatatcggc gttatcgcgc tgttcgagtt cccgcggaac cgtgcggatt
     9721 ttccggtcga ggtcgctgcg tttgagcagc ggcagggttt tgacagcttc cggcgtgtcc
     9781 ggcgttttct gcatttcgag caggcgggcg gcgatgcgga cggtgtccgc cagcgcttcc
     9841 gggctcatgc cgttgcggac ggcctgcagc cgggccttct cttcggcttc gtcgcgcgcg
     9901 gctttgtctt tgtccgggat gagccggacg atggcgcgat ggggattttt cagcagcagg
     9961 gtttcgatga gttcttcgaa gtagcgcggg ttggcggcga tgttggtttt gagctcggcc
    10021 aggggctgct cgtaggcggc gaggccgacg ggatcccggt cgtagagcca ggtttccagc
    10081 atgttgagca tgatggaaag gccctgcggg aagctgccgt ggttgttttc gcgcaggtcg
    10141 aattcaaagg agttcatcgc ggcttcgata tcgccgcggt ccaggccgtc gtgcgccagt
    10201 ttttccagcg tccggaaaat cagcgcctcg actttttcga ggttttcgat ttcgacgcct
    10261 ttcatgccga tggaccaggc gggctggcgc aggctggttt cgagtccgaa tccggccagg
    10321 tcttccccga ggccggattc gatcatcgct ttgcgcaacg gcgaaccgga ggttccggtg
    10381 aggatctggt cgagtacggt cagcgcgaag tgcagggcgt tgtcgggcat ggcccagttg
    10441 acggtgatga acgcatgctc atcgccttcc tcgctgacgg cgtatttttc ttcgacgtaa
    10501 acgggttttt cgaagggcgg ctgcagcggg acggaggaat ccggcggatt ttccaggcgc
    10561 tggaagccgt cgagatattc gctgaggatt ttcaggcggt gttcggggtt gtcgttgccg
    10621 tagaaaaaaa tgcgggcgtt cgagggatgg tagtgggttt tgtggaactg gcagaacgcc
    10681 tcataggtca gcgtcgggat ttccgccgga tttccgcccg agtcgaggcc gtaggtgatg
    10741 tccggaaaga gcgcctgctg ggaccgctcg agcagaatgc tgtcgggcga ggagtaggcc
    10801 cctttcattt cgttgtagac cacgcctttg caggtgagcg gggcttcggg gctttccagt
    10861 tcgtagtgcc agccttcctg ccggaaaaaa tccggggtga tgcgcgggaa aaagacggcg
    10921 tcgaggtaga cgtcgacgag gttgtagaag tcctgctcgt tctggctggc gacgggatag
    10981 acggttttat ccggataggt catggcgttg aggaaggttt gcagcgaacc tttcaacagc
    11041 tgaacaaagg ggtctttgag cggatagttg cgcgaaccgc acaggacggt atgttccaga
    11101 atgtgcgcaa cgccggtgga atcggcggga ggggtgcgga aggtgatgcc gaacactttg
    11161 ttgtcttcgc cgttttcaac ggagatgtat tcggcgccgg tcggttcgtg gcggtactct
    11221 ttgacggtgc cgttgaactc ccggacgaac tgttcgcgga taagcttaaa attgttcatg
    11281 gttaaggcgg tgcagattag agaggcggcc gcgggcaatc aatgttaatc acggtttatc
    11341 aggagccgga cggcgtactg aatgccgcga aggacaaata caagcgcaac cgcaccggcc
    11401 atgccgacga tgtccacgca caggtcgctc aaggctgcgg cgcggtcgat ggtgatgaat
    11461 tgcagtgttt ctgtggcggc ggcgaagaat gcgagcccgg cgaataccgc tgttatacgc
    11521 cgcaatgcag gcggagcggc aatccatgaa agagacgtca gcagggccag ccagctgaag
    11581 agtataaaat gaccggtcgt gtgcacctga tcaacgagtg ttcctgccag agcaatgaac
    11641 ggggtttctc ctttcggctt ggacggagct gacggagccg gtgatgtttt tacggcctga
    11701 accggcggcg gtggagcggt tttatggatc agttcttttg cggtatgcac ggttttttca
    11761 atggaggaat cgaggaattt tccgggcaga acaatgccgg tcataatcag gattaaagtc
    11821 agcatgatca gaaatccatg gcggcgcatc cagggtttca aagcaaacag gcagaggccg
    11881 aatgctgttg tccataaaac accgaagaac agtttccacc acggtgcaga cgggcgttcg
    11941 cagaccggga tgacgcagac gtcgtcaatc tgcatggtgc ctgcgattcc aagattttga
    12001 atttgcaggc gggcatcgac agcgccttcc gggacgggaa agaaatcttt ataatgatgc
    12061 cagcctctgt cttttgggat atccatgacg ccatggtgca gtttgaacag actttttgct
    12121 tgagcgtcgt tgtagaaaaa aacagcccgc gggatgtggt agccttcttt tccccggaca
    12181 actccatgcg cggcagcccg cagtgataca cgaaaagccg gtatgttggt caagtcatga
    12241 gcggtgaaac gtaaaatgcc gtgctgattt gaggaggcgg tcagtacaac tcccggagaa
    12301 ccgccaaaac cgccggcagg aaccagcttt gtccattcat tccatccgct ccagacattg
    12361 gttgccgcag gtgtgctgaa gctgccgtcc ggaatcagtt ccggcccgac gggcctccac
    12421 ggttcataga tattgaaaaa cagcgccatg gtgccgacgg tcagaacggc gaagacgcct
    12481 gcgaaaaggt ttcgtttgct gatggttgcc tgcatcggta aacaatctct caaaagattc
    12541 ttttcagcgg aagttttata ctggaaatta agcggctgca tgaaaaccta cttggacgaa
    12601 tataaagcct tcatttttga cctcgatgga acgttgatca actccgaaaa gtatcacgcc
    12661 gacgggttcg cctatgccgt tgagacgctg agcggctacc ggctgactcc ggccgagcgg
    12721 cgcgagtttt ttgagggaca cacccatctc ttcaccccgg ttctggcgga gcggcacggg
    12781 ttgacactcg atccgaacga ggtgctggcc cgcaagcggg tgcatgtcga caagcacttc
    12841 aaagtggaag tcctgcccgg cgccgaggca ttcattgaaa aatggcgcgg acagatgcgg
    12901 ctggcactgg ccagcaactc gccgcgcccg ttcgttaaac aggctctcgt cggggccgga
    12961 ttgatcgatg tcttcgaatc cgtcatcacc gccgatgacg tgacgcaccg caagcccgac
    13021 cccgaaatgt ttattttaac gctggaaaat ctgcgcctga aaccggacga cgtgattgtt
    13081 tttgaggaca gtccggccgg cgtggccgcc gctcaggcgg ccggctgccc ggtggtgatg
    13141 attgaaaacg gcgcgggccg gtctgtcgaa ggcgttccgc gatatacctg gaaggagctc
    13201 aagcagtttt gaaaacgaca ccgcttcacg gagaacacgt tgcgctgggt gcgcgcatgg
    13261 ctgaattcgg cggatgggac atgcccattc agtacgaagg cattctggcc gaacacgaac
    13321 acacccgcac ccagaccggg ctgttcgata tctgtcacat gggcgaactc gaactgaccg
    13381 gcccgtccgc cgccgccgac ctcgagcggc tgctgaccat gaacgtttcc acgctggccg
    13441 ccggccagtg ccgctacggc tttctgctca acgaacaggg cggcgtgatc gacgacctga
    13501 cctgctaccg gctcggcgaa gagcgttaca tgctcgtcgt caacgcctcc accgccgaag
    13561 gcgatgcggc ctgggtccgg cagcatcttt cgccgcaaac cgggtttgcg gatctgtcgg
    13621 acggcatcgc caaactcgac gtgcaggggc cgacctcgcg cgccgagctc gaaaaagttt
    13681 tcggtcaaaa gctgccggat ctcggctact tccgggcgga atatgtgcag tgtctgggaa
    13741 ccgaaatgct cgtcagtcgc accggctaca ccggcgactg gggctacgag ctgtatttcc
    13801 cggccgccga ggccgtccgc atctggcgcg ccctgctggc caacggaaac atcaaaccgg
    13861 tcggcctggg tgcccgcgac acgctccgcc tcgaaatggg ctactcgctt tacggccacg
    13921 aactttcgct cgaccgcacg cccgtggcca ccagccgcgg cgcatttatc cgcaaagagg
    13981 gcggcttcat cggcgaagcc gccgtgcggc acgatctggc cgcgccgcgc gaatttctgg
    14041 tggcgctgga attcgacagc aaacgcgcgg cccgcgccca cgacaaggtg ttttccggcg
    14101 gcgcagaaat cgggcaggtg accagcggtt cggtttcgcc gagtcttggc aaagccgtgg
    14161 cactggcctt tgttgccgcc ggctgcgcgg ccgttggcaa ggtgctcgac gttgaaatcc
    14221 gcggaaagct gttctccgcc aaagtggtgg agctgccttt ttataagaac gggactgcga
    14281 ggggctgatg catcgacaac tgcttgtttt tgatctggat ggaacgttgg cggactcgcg
    14341 ggccgatctg gccacggcgg tcaacctgat gcgggcgcac tacagtctgc cgccgctcag
    14401 tctggataca attgaaagtt atgtcggcaa cggcgtccgc cagctggtgg cgcgttcgct
    14461 gcaggacacc ggagtggatg tggccgaggc gctgccgctc tataaacagt tttatacgga
    14521 acacatgctc gacgcgaccg ccctctatcc gggcgtcgcc caggggctgc gccggctggc
    14581 ggaatcgggc cacctgctgg cggtcctcag caacaagccg ggcgatccgt cgcgcaccat
    14641 cctccatcac ctcggcgcgg gcccgctgtt cgcctggatt ctgggcggcg gcgatctgcc
    14701 gaacctcaag ccggcaccgg acggcctgct ggcgctggtt gcggcaacgg gcatgccgcc
    14761 gcaaaaggtt tggatgatcg gcgaccacca tactgatctg gaggccgccc gcaacgcggg
    14821 ggtgaagagc ggatttgtca cctacggcat cggaaacccc ggcggattcc gtgccgatca
    14881 ggtctggaac agttttgaag aactggtaga tttctttgcg aataaacgga gtgtgctatg
    14941 agtaatactg aaggactgaa gctgctgaaa aaaggcgaga tgaactatcc gaagaagccg
    15001 gatgcttcga ttctggagac tttcaaaaac tccaacccgc agcgcgatta ctggatcacc
    15061 tttgaaacgt ccgagttcac ttcgctctgc ccgattaccg gccagcccga tttcgcgacc
    15121 atcaaaatcg agtacatccc gaatgaactt tgcgtggaga gcaaatcgct caagctgttt
    15181 ctgttctcct tccgcaacga aggctcgttc tacgaagacg tcaccaaccg catcttcacc
    15241 gacctgacga acgtctgcaa accgcgccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15361 nnnnnnnnnt gccgggtcgt agcagctgga tgttttgccg tagtccacgc ccagctccac
    15421 tcccttttga ataatctgcg ccttggtcag attaatcagc ggcgtatgaa ccgtcagttt
    15481 ttccgatccg gtcacgccgg ccttcgtggc gaggttggcc attttttcgt aggcggcgat
    15541 aaactccggg cggcagtcgg ggtagccgct gtaatccagc gcgttgacgc cgatgaaaat
    15601 atcgaagctg cccagcacct cggcccacgc cagcgcgtac gagaggaaaa ccgtgttgcg
    15661 cgccggtacg taggtgatcg ggatttcgtc cggacggccc ggtgcgtgcg gcacggccag
    15721 cgtcggatcc gtcagcgccg agccgccgaa cgcgccgagg tcgatggtga cggtccggtg
    15781 ttcgcagacg cccagcgctt cagctgtctg cttcgcgcac tccagctcga cggcgtggcg
    15841 ctggccgtag cggaaggaaa gggcgtagac gtcaaactcc tgcgatttgg caatcgccag
    15901 cgcggtggcg gagtcgagcc cgccgctcaa aagaacgacc gcttttttct tcatagcaga
    15961 cccatcatct tgtggcactg gggaataacc cggacgctgc gtccgagtgc gacggccatt
    16021 tgcgactgcc agcccagcac ctgtttcgcc gtcggaaccc ggtcggtttt agaggccgca
    16081 gtcatcggtt gcagaaccac ggcggccggc gaaccggagt cgcggatgat accggcggcc
    16141 tgcgcgagat cgccgtcggc ggtgtccgcg ctgaccacca gtttaatggt cgcgccggtt
    16201 tcttcgcgct cgatgatctc cagaaaatcg cggtgcatcg cccaggtttc cggctcgccg
    16261 gtaacgctgg gcagtttgat gtccatcaaa acgtggtcaa cccactcaac gacctgttcg
    16321 aacggttccg gcagatcgcc gctggtttca aggtgtaccg gcagatcgag ctgcttgcgg
    16381 gccgccggca gaaacgccgc cagaaactcc gcctgcagca gcggttcgcc gccggtaatg
    16441 aacaggtcgt cgtgggcgaa ttccgggcgg ctcaaaccgg ccagcagctc cagcagctgg
    16501 tcgacggaaa gcggattggg aatggagtca aactgcccgc tgcccggttc gcgctcgatg
    16561 gccgcggcgg cggtccgcgc aaccggcgtg tcgcaataaa ggcagttgcg gtgacactcg
    16621 cagaaccgga cgaaaagctg gcggcggccg atgttgaccc cttcgccctg aaccgagctg
    16681 aatatctcgc tcacaaaagc tgttattttg tctgttttgc tcataaatta cccggccttg
    16741 agtaccacca agcgggccaa taaccaacaa ctaataacca ataaccggcg tccatgaaag
    16801 tagacctttt tgattttgag ctgccgcagc acttcatcgc ggaccgcccg gccagcccgc
    16861 gcgactccgc acgcctgctc gacctgaccg gcgccggttt cgccaaccga attgtccgcg
    16921 acctgccgga actgctccag cccggcgacc tgctgatcgg caacaacacc aaggtgattc
    16981 cggcgcggct cgacggcgtg cgcggcgaag tgaaaattga agtcaccctg cacatgcgcg
    17041 cctcggataa tgaatggttt gccttcgcca agcccggcaa gcggctgaaa aaagacagcc
    17101 tgatcgactt cgccgacgac tttttcgccg aagtggtcga aaagcgtgac ggcggagaaa
    17161 tcctgctgcg gttcaaccgc cgcggggcgg cgcttttcga gtgccttgaa aaatatggcc
    17221 ggatgccgct cccgccgtac atccagcgca agcgcgacga cgaacggacc gccagcgacc
    17281 gcgaaagcta tcagactgtt ttcgcccggc acgaaggcgc cgtcgccgca ccgaccgccg
    17341 gactgcactt tacgcccgaa ctgtttcagg cgctgtccaa acgcggtgtc ggcttcgaca
    17401 ccgtgacgct ccacgtcggc gccggaacct tcctgcccgt caaagtggac aacaccgaag
    17461 accataaaat gcacgcggag tgggggacgg tatcgcagga aacggtcgac cggatcaacg
    17521 caacgcgcct ggccggcgga cggattgttg ccatcggcac cacctcactg cgcctgcttg
    17581 aaagcgccgc gacggcaccg ggagagctgc agcccttcgc gggcgaaacc tccatcttta
    17641 tcacgcccgg ctatcagttt aaaatggtcg acctgctgat gaccaacttc cacctgccgc
    17701 gctcgacgct ctttatgctc gtttccgcct tcgccggact cgaccggatg cacgccgcct
    17761 acgaacatgc taaaaacgaa ggctaccgct tttactccta cggagacgcc tgcctgctga
    17821 aaa
//
LOCUS       DAOPZO010000026        17730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_3352_length_17730_cov_8.475983, whole genome shotgun sequence.
ACCESSION   DAOPZO010000026 DAOPZO010000000
VERSION     DAOPZO010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 17730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17730
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3352_length_17730_cov_8.475983"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(324..399)
                     /locus_tag="PKI68_03135"
     tRNA            complement(324..399)
                     /locus_tag="PKI68_03135"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Ala,seq:cgc)
     gene            588..2303
                     /locus_tag="PKI68_03140"
     CDS             588..2303
                     /locus_tag="PKI68_03140"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNX52720.1"
                     /translation="MKFTELGLSPEILEGISRLGFETPTPVQAAVIPAILSNGRDLVA
                     LAQTGTGKTAAFGLPVLQMLDTDIKTPQTLILCPTRELCMQIARDLENFAACVRGVRV
                     LAVYGGADIRQQLSAMVRGVDIVVATPGRMVDLLRRKRADFSKIKRVVLDEADEMLNM
                     GFEEDLEAILSEVPDGAQTLLFSATMPRQVASIAKKYMKDPEEITVGTRNAGAENVSH
                     EYLVVHAKDRYRALKRLADFYPDMYGIVFCRTRQETQDIADHLGKDGYKAESLHGDLN
                     QPQRDRVMKRFRARELQMLVATDVAARGLDVNDLTHVINYSLPDDLSSYTHRSGRTGR
                     AGKTGVSIALINMREHSKVKHIEKLLGRKFVQRDVPSGADICKARLLGLMERLSQVNP
                     EPGLIDEILPSLCESLEGVSRDELLRRFASMELQRMLDYYSKEPDINAEPLHEGSHAA
                     RKKQTAAGGLVELCMNIGKANRLSPKQLMNLVNVADRNSSVEIGRINITHMQSYFEVP
                     RDAAQGVIDSFANSLVDFEGRKVSVSLAGAGNPAKRDEKRAANPYRPNRPKNKPGQKP
                     GHWKK"
     gene            complement(2304..4841)
                     /locus_tag="PKI68_03145"
     CDS             complement(2304..4841)
                     /locus_tag="PKI68_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNX52721.1"
                     /translation="MKNKLKKSNSLDPHSIRPGRLFLHTLRLTGFALGLSVLLALFAL
                     LAVGLPPGLTNRITARIREAGVPLQVESIRLSTHRGWVLSNARLYSTSPDDLQPLLSA
                     KKLYIMVWPVDWRNIAGGDWRIKLFVRDLGVSLGRPWENTLQGTHPFRTVSKLDATLT
                     ARSEHITVENAELQWGGVTILAHGTADFSDNAILAGGGETELQQSKADFRRSAAKAAN
                     ALSQLQCKQAPQLNLSFNFNEARPDETFLEAFLSVGGITLQDRVYNQLSGAFGYRNNT
                     WTLSALQLNRSNREQLTVRGEVNLANSNAQVSVESTLSAADLFSLLPEDAQSAVAQTG
                     VKPYGKCDFTASFGPAPYDLLTEKVDVQVQQVQFKRDDLTVDPLALHFLRDGSRVELE
                     NIQARVNGGPVGGSFKFDLESKAWIARVQAQSNNPKPIGTLAGDDDLAEFISRFRFPA
                     EPFKADLTISQAAPGANLFVAGTLSGDRFTCAGVPISHLETFMVYSNEVLNLNPLHIT
                     RTNEQFAGTVRVDFVNGLGFFNATNSFPPADIAHILAPDEHTVLEQFTFNGPVYAAGQ
                     GQLDYRHWTNHSFSGSFRAESIGFEKVQASLLHADIKGRGTQLIFTNASMQFYGGKAE
                     GSAEFDLLLEDGSAPYSLNVGITGLNMEQMLEQISSGDYSHTRGQLSASINCTADAKA
                     GFWKSVKGGGKVEIKDGRLADVPLFGGFSRLLQSAFPAFNLFSLTAFAADYELHDGAV
                     WSENAQLGGTLVSARGRGHYSPEKGLDFTIAAEPLRQTGSDKEKNQLQRLAATALKEG
                     TAPLFRLLEFKLEGPLDKPEWRFVNLPKEVSDLPKEVSGLLRRSKDK"
     gene            complement(4807..6591)
                     /locus_tag="PKI68_03150"
     CDS             complement(4807..6591)
                     /locus_tag="PKI68_03150"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNX52722.1"
                     /translation="MKHKVTNWMLLIGAVLLGGFILIFERGSENSQQQNQRSRTVFSV
                     HPESIEQILMERDGVQIECTKTAGIWRLTKPADAPVDSGVVEKMISGLTRVERGELIA
                     AKTLQERNLTPAAYGFDKPRARITFRNNHGTFTWLIGRDAPIGKTLYVMPADGGDIIS
                     APRTLLNLIPQDPAWIRDRTLFSGETAAVRGLDLRRTAGFLQLRQPENNGWTILQPYT
                     GRADSQSVHALIGKIFAGRIIDFVTDEKADLTAYGLEKPAYELTVFTQDERTQTLLVG
                     KPVPEKPETRYAKRVETDSVFTVPADWTKEFEADAGLFRSRQVLGMQPDRIVAIQLIH
                     GEQQIGLVRTNDLWQVIRPVRWDADPEQTAEVLKALTGASVEEFIDAPSAVQTAQMAA
                     APWKAVVSADGKTGTLHISEPGTNGLRMVQCNDAPVFCTTTNGIVRDTFTDPLFYRSR
                     KVLEVNPALVQKIIVQTGGTEQSIQKTETGSFAATQSNRQVNSGALTDLMWAFNDLRA
                     ERYVNFNPVSLKPYGLDQPQTSLTVTLSDTNVIGRMVLLGGKTDGGRFAMIQGQNIVF
                     VVSDETAQTLTRELTVPIEKQAEEIKQP"
     gene            complement(6596..8035)
                     /locus_tag="PKI68_03155"
     CDS             complement(6596..8035)
                     /locus_tag="PKI68_03155"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HNX52723.1"
                     /translation="MKNRRHRLWITLSTGAALLLALVIALMVNYLSFRHYYRTDWSRA
                     QLYNLSSKTTGLLESLDKPVSVTVFFQPGNVLYDDIHNLLREYQFHCSRLNIQWVDPD
                     RDISQTEELAVKYKVTDPNVVVFDCGGRSKYVRADEIANINNASGIDRITSFKGEQAF
                     SSAIQGVVQKTVPAVYFLTGHGERDITDFDRRTGFSGAAQLIERDNIAVKPLLLSTVK
                     QIPADCSALIVAGASQSMSKSEADLISVWLRRSGRLMVLTDAGQTSGLEKLIQEWGVR
                     LRNDVVIDPDRTLTGREVYVSAYNRHSITAKLGTTAAIFHLPRSVEPDFTQTKTASAD
                     RPLVTALALSSKKSWAEAQPDQVPAKYDAGTEDLPGPVSMAVAVEKGDTTGLLDMQIR
                     PSRLVVFGDSGFVSNSGLTGGDASFFMSALNWLLDREQLMAIPPKELDDTRLKLTREK
                     IRFLSWSTIGVIPALAALLGTVLWFRRRK"
     gene            complement(8048..8779)
                     /locus_tag="PKI68_03160"
     CDS             complement(8048..8779)
                     /locus_tag="PKI68_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX52724.1"
                     /translation="MRTFFALWRRELAALFLSPIAYVMLMFFLLVTGCGFYWLVKENL
                     EVMRSVQGLMVVIWACMLIVIPILTMRTFAEERKNGTFETMMTAPVKDSTVVSAKFAG
                     VLTFFVLMCIPTLLYLVIIRLLAPEAAHLIEAGPIASGYLMVGLIASAFIALGMLASS
                     LTSNQIIAAISTFSVMSILFFGGLFEPHLSKNPLVREVGGYLSSALHLMESARGVFDS
                     RSVILYLSVTLFFLFATVKAVEARR"
     gene            complement(8779..9726)
                     /locus_tag="PKI68_03165"
     CDS             complement(8779..9726)
                     /locus_tag="PKI68_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX52725.1"
                     /translation="MIKVSNLTKQFSGHTAVDDVSFEVSRGEIIGFLGPNGAGKTTTM
                     RMLTGFLHPTRGGVEIAGVDVFEDPIEARRHIGYMPESCPLYTEMRVDEYLTFRAQIK
                     GVARRAIRGRRDIVKEQCGLTDVGRRIIGQLSKGYRQRVGLADSLLHDPDLLILDEPT
                     AGLDPGQIREVRELIRQLAERHTLLLSTHILPEVEMTCRRILIINKGKIVASDSPESL
                     QKRLEGDVLISAEIAGDPAAIEAELKNLGTVDVVSHELLGSGWNRYRIDSSAPGIRSQ
                     VFDCAVANGWKLRELHLESRSLEDIFVAIIRDDLATEAK"
     gene            complement(9823..10524)
                     /locus_tag="PKI68_03170"
     CDS             complement(9823..10524)
                     /locus_tag="PKI68_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX52726.1"
                     /translation="MKNETKKNGPLILVIDDEASVRRMLRYALEGHGYRVQETATGET
                     GLTEAATCSPDTIVLDLGLPDMDGAQVLRRLREWSAVPVLILSVRDEPDDKVDALDAG
                     ADDYVTKPFDTEELLARLRAIQRRARTGESAPVIVVGKLEVDMPSHAVRIHGQELKLS
                     PIEFSLLRVLAQHAGRIVTHRTILREVWGPKSEEQSQYLRVHFTHIRRKLKEAGLESP
                     SVINEPGIGYRLAGE"
     gene            complement(10521..13160)
                     /locus_tag="PKI68_03175"
     CDS             complement(10521..13160)
                     /locus_tag="PKI68_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HNX52727.1"
                     /translation="MNKPFRPDPDALLAELNRQASQQGRLRIFFGMCPGAGKTYAMLQ
                     AARQRKAEGVDVVIGLVETHGRPETTALLEGLEVLPRTCINYRGTVLKEMHLDGLLLR
                     KPRLAVVDELAHTNAPGSRHPKRCADVLELLEAGIDVYTTLNVQHVESRAPVVRQVTG
                     VTVRETVPDSVLDRADEIELIDISPEQLRKRLAEGRVYLGDRAAAAGENFFRESNLTA
                     LREMALRLTAERVDQGMREHMREQRIAGPWKARERLLVGVGPSPFAGDLVRWTRRIAD
                     ALDATWMAVYVDDGHPLTEEEKKRVTEFLALARRLGGEVESVSGSRVAGELMRVAREQ
                     NVTQIVVGKPRQSLWMRLLSGGSLVDELLHRSGDIDVYVVRPEKSAEAVRTSRTREGV
                     HFREWGWGLLLLAGVTLAGWMLCDWVGYLSVALFYLLMTVTGALFLRRRVVLVTAAAG
                     AFLWNLLFVPPLFTFRIAQFHDAMMFFLLIVVALVMGHLTTRMRQREEGERQRQKSTA
                     ALLKLTRSSALNPDMNDGLCEALDQINALFSGKTVLYLRDLNTRQLNAKPHAASLWTP
                     SSKEYSVAEWAFSNRRIAGRFTDTLPGAEGTFWPLQTRTSLMGVLGLRLGFECELNLP
                     ERDLLASFATQLALALEKDHFRAAFQRSEVLDASNRLRRVLLDNVSHELRTPLTALKA
                     ALTALKSIPQTGELLEEAEAASDRLTNMVDLLVDSARLETAQPGVKKEWCEISDLIED
                     TVRLCRKELMNRPVRINLITPDLLVLTDTRLLQQALIHIIRNAGIHTPAGSDIEILAR
                     INNGNLEITVRDHGTGFADPVRAFEKFYRDPHAPAGGLGLGLSITRGLISALNGQVDA
                     CNRPDKGAEVTVSVPVEARMPEA"
     gene            complement(13157..13732)
                     /gene="kdpC"
                     /locus_tag="PKI68_03180"
     CDS             complement(13157..13732)
                     /gene="kdpC"
                     /locus_tag="PKI68_03180"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF001454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HNX52728.1"
                     /translation="MKQILIPALRLLLMMTLLTGVIYPAAVTWAAHLLCPETAQGSLV
                     YRDNRVVGSALLAQKFESPGYFRSRPSAAEWSTLPSGASNLGPASGKLQEQIKERADV
                     WRDLLALPAKTPVPSDLLAASASGLDPHITLEAARFQVARVAQARRLPFAAVEDILQK
                     HLENARPGSPGVPYVNVLLVNMELDRIHSAP"
     gene            complement(13744..15819)
                     /gene="kdpB"
                     /locus_tag="PKI68_03185"
     CDS             complement(13744..15819)
                     /gene="kdpB"
                     /locus_tag="PKI68_03185"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HNX52729.1"
                     /translation="MTTRSSIFDHSLLRRALLDSLRKFDPRVQLRNPVMFVVFIGALA
                     VSAVSAHTPSAFNLQIALWLWFTVLFANFAEAMAEGRGKAQAAALRRMRVSLKARRLR
                     NGREESVAAAELKKGDEVLCEAGDTIPTDGEIIEGVASVDESAITGESAPVIRESGGD
                     RSAVTAGTRVISDRIKVRVTSEPGQTFLDRMIALVEGSVRGRTPNEQALGVLLAGLTF
                     IFLLAVVTLPAFLGYAEREGGVKTGGLLSLPVLVSLLVCLIPTTIGGLLSAIGISGID
                     RLSRRNVLATSGRAVEAAGDVDVLLLDKTGTITLGNRMAVDFLPARGVDANRLADAAQ
                     LASLADETPEGRSVVVLAKQLFALRGRELADRHAHFVPFSAQTRMSGVDLPADAEHPA
                     RSIRKGAAGAIRQHVEQQDGIWPADVEAHVETISRQGGTPLVVCEDSHVLGVIFLKDV
                     VKGGIRERFAQLRRMGIRTIMITGDNAATAAAIAAEAGVDDFLAEATPEDKLNRIRAE
                     QADGHLVAMTGDGTNDAPALAQADVGVAMHTGTQTAREAGNMVDLDSSPTKLLDIVEI
                     GKQMLITRGALTTFSVANDVAKYFAILPALFLGLYAGTGSATGPLAALNIMRLASPES
                     AVLSAIIFNALIIVALIPLALRGVAYRATGASLLLRRNLLIYGAGGLAAPFLGIKLID
                     LALTALGWI"
     gene            complement(15829..17517)
                     /gene="kdpA"
                     /locus_tag="PKI68_03190"
     CDS             complement(15829..17517)
                     /gene="kdpA"
                     /locus_tag="PKI68_03190"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HNX52730.1"
                     /translation="MQTTDILLFGAFAVLLAVLSPWIGRWIANVMDGSPGRLPGFLRR
                     LERLIYRGSGINPSETMDWQHYTGALLAFNLTGFIAVMVLQLLQNRLPFNPQGLPAVS
                     WPLAFNTAVSFATNTNWQAYSGESTLSYLTQMLGLTVQNFVSAATGIAVAFALARGLA
                     SRSARELGNFWCDMTRATVYILLPLSAVLALILAGQGVVQTFHSYVEAHTLEGAVQMI
                     PLGPAASQIAIKQLGTNGGGFFGVNSAHPFENPTVFSNLLQLWAILLIPAALPFAFGR
                     TVRNPRHGACLFAAMLTMFLAGLALTYTAELQLAGRSAAGIWEGKEVRHGLASSVLWA
                     SATTAASNGSVNTMHSSLSPLAGMAALFNIMLGEVIFGGVGAGLYGIIAFVILTVFLA
                     GLMVGRTPEYLGKRLDRGDILLASVAVLAPCVVILLGCGITMIWTGTLDSRANTGPHG
                     FTEILYAWSSAAGNNGSAFAGLNANTGFFNIGLGIAMLIGRYAVIVPMLALAGRFAVR
                     KITPPSSGSFPTDGATFAVLLIAVILIVGGLTFLPALFLGPAADHLLWISGRTL"
BASE COUNT         3701 a         5133 c         5316 g         3580 t
ORIGIN      
        1 cggcgccgtc ctcgaccgca aggcgtttga gcatttcaag taacgttaag cgcgttaaac
       61 gcgctgaaca ccggcgccgc cgcaggcggg gcaggttttt aagatggagg tgaagattcc
      121 cccggtgtca atctcgccgg tacctttgca gcgtgcacag gggacaatct tcgcaccgcc
      181 gggcacatta aaactgttgt tgaatcgcaa gcggcgggag cacttcggac agagcatgta
      241 gttgccattc agtttatgac cgcaatgagg gcaatagttc atattgtttt atcaaactta
      301 tgcatttaca ccgttgcggc gaatggtgga gcggaagggg atcgaaccct cgacctccac
      361 gttgcgaacg tggcgctcta ccaattgagc taccgcccca ttaaaggagc gccgaatata
      421 ggcgggctct tttcgccggg caagttctaa aactctcccg aacaattaag ttgatctttg
      481 gccggcctgc ccccatcctc cgcgcgcctg aaattttcgg gccccggtgt ttacttgcag
      541 gaatgaagat cggtatttgg gttcctgcag caaacatggg tggttcaatg aagtttaccg
      601 aactggggct cagccccgaa attctcgaag gcatttcccg ccttggtttc gaaaccccga
      661 cgcctgtcca ggcggcggtt attccggcga ttctcagcaa cggccgcgat ctggtcgcgc
      721 tggcacagac cggtaccggc aaaaccgccg cgttcggtct gccggtgctg cagatgctcg
      781 atacggacat taaaacgccg cagacgctga ttctctgtcc gacgcgcgaa ctctgtatgc
      841 agatcgcccg cgacctcgaa aatttcgccg cctgtgttcg cggtgttcgc gtactggccg
      901 tttacggcgg agccgatatc cggcagcagc tttccgcgat ggttcgcggc gtcgacatcg
      961 tggtcgccac gccgggccga atggtcgatt tgctgcgccg caagcgcgcc gacttttcaa
     1021 agatcaaacg cgtggtgctc gatgaagccg acgaaatgct gaacatgggc tttgaagaag
     1081 acctggaagc gattctttcg gaagttcccg acggcgctca gacgctgctg ttttcagcga
     1141 cgatgccgcg tcaggtggcc tcgattgcca agaagtacat gaaggatccg gaagagatca
     1201 cggtcggcac acgcaacgcc ggcgccgaaa acgtcagcca cgaatatctg gttgtccacg
     1261 ccaaagaccg ttaccgcgcg ctgaagcgtc tggctgactt ttacccggac atgtacggga
     1321 ttgttttctg ccgcactcgg caggagacgc aggatatcgc cgatcacctc ggcaaagacg
     1381 gctataaagc cgaatcgctg cacggcgatc tgaaccagcc gcagcgcgac cgcgtgatga
     1441 aacgcttccg cgcccgcgag ttgcagatgc tggtcgctac cgacgttgcc gcccgcggtc
     1501 tcgacgtaaa cgatctgacg cacgtcatca attacagcct tccggatgat ctgagcagct
     1561 acacacaccg cagcggccgt accggccgcg ccggtaaaac cggcgtttca atcgcgctga
     1621 tcaatatgcg agaacacagc aaggtgaagc atattgagaa gctgctgggc cggaaatttg
     1681 tccagcgaga cgtaccgtcc ggcgcggata tctgcaaggc gcgtctgctc ggcctgatgg
     1741 agcgcctgag ccaggtgaat ccggagccgg ggctgattga cgaaatcctg ccgtcgcttt
     1801 gtgaaagtct cgaaggcgtt tcgcgcgatg aacttctgcg ccgtttcgct tcgatggaac
     1861 tgcagcgtat gctcgactac tacagcaaag agccggacat taatgccgag cctctgcatg
     1921 aaggcagcca tgccgcccgt aaaaaacaga ccgccgccgg cggactggtt gagctttgca
     1981 tgaatatcgg caaggccaac cggctgagcc cgaagcagct gatgaatctg gtgaatgtcg
     2041 ccgaccgcaa cagcagtgtt gaaatcggcc ggatcaacat cacacacatg cagtcctatt
     2101 tcgaagtgcc gcgtgatgcc gctcaaggag tgatcgacag ctttgcaaac agcctcgttg
     2161 attttgaagg ccgcaaagtc agcgtgtcgc tggccggtgc aggcaatccc gcgaagcgcg
     2221 acgagaaacg cgcggcgaat ccgtaccgcc cgaaccgtcc gaaaaacaaa cccggtcaaa
     2281 aacccgggca ctggaagaaa taattattta tctttcgagc ggcgaaggag tccggaaacc
     2341 tctttgggca ggtcggatac ttccttcggc agattgacga agcgccactc cggtttatcg
     2401 agcggtcctt cgagcttgaa ttccagcagg cggaaaagcg gcgcggttcc ttctttaagg
     2461 gccgtcgcgg ctaaccgttg cagctgattt ttttctttat cgctgccggt ctggcgcagc
     2521 ggctcggcag cgatggtgaa atcgagtccc ttttccggcg aatagtgtcc gcgtccgcgg
     2581 gcgctgacca gtgttccgcc gagctgcgca ttttcgctcc agactgcgcc gtcatgcagc
     2641 tcgtagtcgg cggcgaatgc ggtgagcgag aaaaggttga atgccgggaa ggcggactgc
     2701 agcagccgcg agaacccgcc gaagagcggc acatcggcca ggcgtccgtc tttaatttcc
     2761 acttttccgc cgcccttaac cgatttccag aaaccggctt tggcgtcggc ggtgcagttg
     2821 atggaagcgg agagctgccc gcgggtatgg ctgtaatcgc cggaggaaat ctgttccagc
     2881 atctgctcca tattgagccc ggtgatgccg acgttcagac tgtaaggcgc ggatccgtct
     2941 tccagaagaa gatcgaattc agcggagcct tcggcctttc cgccgtagaa ctgcatggag
     3001 gcgttggtga agatgagctg tgtgccgcgg cctttgatgt cggcatgtaa gagtgacgcc
     3061 tgaactttct cgaagccgat gctttcagca cggaagctgc cgctgaagct gtggttggtc
     3121 cagtggcggt aatccagctg tccctgtccg gcggcataga ccgggccgtt gaaggtgaac
     3181 tgttcaagaa cggtgtgttc atccggtgcg aggatgtgcg cgatatccgc cggcgggaag
     3241 ctgttggtgg cgttgaagaa cccgagaccg ttgacaaaat ccacccgtac ggtgccggcg
     3301 aactgctcgt tcgtgcgggt gatatgcaga gggttcaggt taagcacctc gttggaatac
     3361 accataaacg tttcgagatg actgatcgga acgcccgcgc aggtgaaacg gtcgccggaa
     3421 agagtcccgg cgacaaaaag attggctccg ggagcggcct gcgagatggt caggtcggct
     3481 ttaaacggtt ccgccggaaa gcggaagcgg ctgatgaatt ccgccagatc gtcgtcgccg
     3541 gccagtgtgc cgatcggttt cggattattg ctctgcgcct gtactctggc gatccatgct
     3601 ttggattcca gatcgaattt gaaactgccg ccgaccgggc cgccgttgac acgcgcctga
     3661 atattttcca gttcaacccg gcttccgtcg cgcaggaagt gcagagccag cgggtcgacc
     3721 gtgagatcgt cgcgtttaaa ctgtacctgc tgcacctgca cgtcgacctt ttcggtcagc
     3781 agatcgtacg gcgccgggcc gaacgaagcg gtgaagtcgc attttccgta cggtttaacg
     3841 ccggtctgcg ccacggcgga ctgagcgtct tccggcagca ggctgaacag atcggcggcg
     3901 gacagggtgc tttcaacaga cacctgtgca ttgctgttcg cgaggttgac ctcgccgcgc
     3961 accgtgagct gttcgcggtt cgaccggttc agctgcagcg ccgagagtgt ccaggtattg
     4021 ttgcggtaac cgaacgctcc ggaaagctga ttgtaaacgc ggtcctgcag ggtgattccg
     4081 ccgaccgaca gaaaggcttc cagaaacgtt tcgtccgggc gggcttcgtt gaaattaaac
     4141 gagaggttga gctgcggagc ctgtttgcat tgcagctgac tcagcgcatt tgccgctttg
     4201 gcagcgctgc ggcggaagtc ggccttgctc tgctgcagct ccgtttcgcc gccgccggca
     4261 aggatggcgt tatcggaaaa gtctgcggta ccgtgtgcga ggatggttac gccgccccat
     4321 tgcagctcgg cattttcaac cgtgatatgt tcagatcggg ccgtcagcgt agcgtcgagt
     4381 ttgctgaccg tgcggaacgg atgggttccc tgaagtgtat tttcccacgg acggcccagc
     4441 gatacgccga gatcgcggac aaaaagtttg atcctccagt cgccgccggc gatgtttctc
     4501 cagtcgaccg gccagaccat gatataaagt ttttttgcgc tcaggagcgg ctgaagatcg
     4561 tcgggcgatg tgctgtagag ccgggcgttg ctgagtaccc agccgcgatg cgtcgagagc
     4621 cggatggatt caacctgcag cgggactccc gcctcccgta tccgggctgt gatgcggttc
     4681 gtcagaccgg gcggcaggcc gaccgccagc agtgcaaaca gcgccagcag aacggagagt
     4741 cccagcgcga atccggtcag acggagtgtg tgcaggaaca gtctgcccgg gcggatgctg
     4801 tgcggatcaa ggctgtttga tttcttcagc ttgtttttca ataggcaccg tcagttcgcg
     4861 ggtgagagtt tgtgcggttt cgtctgaaac cacaaacacg atgttctggc cctgaatcat
     4921 ggcaaaacgt ccgccgtcgg ttttaccgcc gagcagcacc atgcggccga tcacgttggt
     4981 atcgctcagt gtaaccgtca gcgaggtttg cggctgatcc agcccgtagg gtttgagtga
     5041 aaccggattg aaattcacat aacgctcggc gcgcaggtcg ttgaacgccc acatcagatc
     5101 ggtcaacgcg ccagagttga cctgccggtt ggactgagtg gcggcaaaac tgccggtttc
     5161 tgttttctgg atggattgtt ccgttccgcc ggtctgtacg ataattttct ggaccagcgc
     5221 cggattcact tcaagcacct tgcgactgcg gtagaaaagc ggatcggtga acgtgtcgcg
     5281 tacaatgccg ttggtcgtgg tgcagaacac gggtgcatca ttgcactgaa ccatgcgcag
     5341 tccatttgtc ccgggctcgc tgatgtgcag tgtgccggtt tttccgtcag cggacacaac
     5401 ggctttccat ggagcggccg ccatctgtgc ggtttgtacg gcggacggcg catcaataaa
     5461 ttcctcaacg gaggctccgg taagcgcctt cagaacttcg gcggtctgct cgggatccgc
     5521 atcccagcgt accggccgga taacctgcca aaggtcgttg gtgcgaacca gaccgatctg
     5581 ctgctcgcca tggatgagct gtatggcgac gatccggtcc ggctgcattc ccagcacctg
     5641 ccggctgcgg aacaggcctg catcggcttc aaattctttc gtccagtcgg caggcacggt
     5701 gaaaacggaa tcggtttcaa cccgtttggc atagcgtgtt tccggttttt ccggaaccgg
     5761 tttgcctaca agcagcgttt gcgtacgttc atcctgcgtg aaaacggtca gctcataggc
     5821 cggtttttcc aggccgtagg ctgtcagatc ggctttttcg tccgtgacga aatcaatgat
     5881 acgtccggcg aaaattttcc cgatcagtgc gtgtacggac tggctgtcgg cgcggccggt
     5941 gtagggctga aggatcgtcc atccgttatt ttccggctga cgcagctgga ggaacccggc
     6001 ggtgcggcgc aggtcgagcc cgcgtaccgc ggcggtttcg ccggagaaaa gcgtccggtc
     6061 gcggatccac gccggatcct ggggaatgag gttcagcaag gtgcgcggcg cggagatgat
     6121 atcgccgccg tccgccggca tgacgtaaag cgtttttccg atcggcgcat cccggccgat
     6181 cagccaggtg aaggttccgt ggttgttccg gaaggtgatg cgcgcgcgcg gtttatcgaa
     6241 accgtacgcg gcgggagtga gattccgttc ctggagtgtt ttggcggcga tgagttcgcc
     6301 gcgttcgacg cgcgtcaggc cggaaatcat tttttcaacg acgccggaat caaccggcgc
     6361 atccgccggc ttggtaagcc gccagatgcc ggcggttttg gtgcattcaa tctgcacgcc
     6421 gtcacgctcc atcagaatct gctcgatgct ttcagggtga accgagaaaa ccgtgcggct
     6481 gcgctgattc tgctgctgcg aattttcgct gccgcgctca aagatcagaa taaatccgcc
     6541 gagcaggacg gcgccgatca gaagcatcca gttggtgact ttgtgtttca tgaagttatt
     6601 ttcttctccg gaaccagagt acagtgccca gcagagcggc cagcgccgga atgactccga
     6661 ttgtgctcca ggaaaggaac cggatttttt cgcgggtcag tttaagccgg gtatcgtcta
     6721 attctttcgg cggaatcgcc atcagctgtt cgcggtcgag cagccagttc agcgcgctca
     6781 taaagaagct ggcgtcgccg ccggtgaggc cgctgttcga aacgaagccg gaatcaccga
     6841 agacaaccag ccgtgacggg cggatctgca tgtcgagcag accggtcgta tcgccttttt
     6901 caacggcgac cgccatggag acggggccgg gcaggtcttc ggttcccgca tcatattttg
     6961 cgggcacctg atccggttga gcttccgccc agcttttttt ggaggaaaga gcaagcgcgg
     7021 taaccagcgg ccggtcggcg gaagccgtct tggtctgtgt gaagtccggc tcgaccgagc
     7081 gcggcagatg gaatatggcg gcggtggtgc cgagtttggc tgtaatggag tggcggttat
     7141 aagcggatac gtaaacttca cgtccggtta acgtgcggtc gggatcgatt acgacatcgt
     7201 tgcggagccg tacgccccat tcctgtataa gtttttcgag tccggaagtc tgcccggcat
     7261 cggtcagaac catcagacgt ccgctgcggc gcagccagac ggagatgaga tccgcttcgg
     7321 acttcgacat gctttgcgat gcgcccgcga caatcagcgc gctgcaatcg gcagggatct
     7381 gtttgacggt gctcagcagc agcggtttga ctgcaatgtt gtcgcgctca atcagctggg
     7441 cggcaccgga aaatccggtg cggcggtcga agtcggtgat atcgcgttcg ccgtggccgg
     7501 tcaggaagta aaccgccgga acggttttct ggacgacgcc ctggatcgcg gacgaaaagg
     7561 cctgttcgcc tttgaatgaa gtgatgcgat cgatgccgct ggcgttgttg atgttggcga
     7621 tttcgtcggc acggacatat ttgctgcgcc ctccgcagtc gaaaacgacc acgttcgggt
     7681 cggtgacttt gtattttaca gccagttctt cggtttggga aatgtcgcgg tccggatcga
     7741 cccactggat gttcagccgg ctgcagtgaa actggtattc gcgcagcagg ttgtggatgt
     7801 cgtcgtagag cacattgccg ggctgaaaga aaacggtcac ggaaaccggc ttgtcgaggc
     7861 tttccagcag gccggtggtt ttggacgaga ggttgtaaag ctgtgcgcgg ctccagtccg
     7921 tgcggtagta atgacggaac gagagatagt taaccatcag cgcaatcacc agagccagaa
     7981 gcagcgcggc gccggtactg agcgtgatcc ataaacgatg tctgcggttt ttcataggcc
     8041 attcacttca gcggcgggct tcgaccgcct taacggttgc aaacaggaaa aacagggtga
     8101 cgctcaggta gagtatgacc gagcgggaat caaagacgcc gcgcgctgat tccatcaggt
     8161 gaagcgccga ggaaagatat ccgccgactt cccgcaccag cggatttttg gagagatgcg
     8221 gctcaaagag cccgccgaaa aagagaatgc tcatcacaga gaaagtcgaa atcgccgcga
     8281 tgatctggtt ggaggtcagc gacgatgcca gcataccgag cgcaatgaac gccgaggcga
     8341 tcaggccgac catcaggtag ccgctcgcaa tcgggcccgc ttcaatcaga tgagcggctt
     8401 caggtgccag caggcggatg atgaccagat aaagcagggt gggtatgcac atcagcacaa
     8461 agaaagtgag aacgcccgca aacttggcgg agacgacggt cgagtctttc accggtgcgg
     8521 tcatcatggt ctcaaatgtg ccgtttttgc gttcttcggc aaacgtgcgc atcgtgagaa
     8581 tcggtataac gatcagcatg caggcccaga tcaccaccat caggccctga acggagcgca
     8641 tcacttccag attttctttc accagccagt agaagccgca gccggtcacc agcaggaaaa
     8701 acatcagcat cacataggcg atcggagaaa ggaacagcgc cgccagttca cgccgccaaa
     8761 gagcaaaaaa agttctcatt atttcgcctc cgtcgccaga tcgtcgcgga tgatagccac
     8821 aaaaatatcc tccagcgagc ggctttccag atgcagttcg cgcagtttcc agccgttggc
     8881 cacggcgcag tcaaacacct gcgagcggat gccgggcgcg gaggagtcaa tccggtagcg
     8941 gttccatccg ctgccgagaa gttcgtgcga gacgacatcc acggtgccga gattcttcag
     9001 ctcggcttca atcgccgccg ggtcgccggc gatttccgcg ctgatcagta cgtcgccttc
     9061 cagccgcttc tgcaggcttt ccggagagtc cgacgccacg attttgcctt tgttgatgat
     9121 caggatccgg cggcaggtca tttcgacctc cggcagaata tgggtcgaaa gcagcagggt
     9181 gtggcgctcc gccagctggc ggatcagttc gcgcacttca cggatctgtc ccggatcgag
     9241 tccggcggtc ggttcgtcga gaatcagcag gtcgggatcg tggagcaggc tgtcagccag
     9301 gccgacgcgc tggcggtagc ctttggagag ctggccgata atccggcggc cgacatcggt
     9361 gagtccgcac tgttccttga cgatgtcgcg ccgtccgcgg attgcccggc gcgctacgcc
     9421 tttgatctgt gcgcggaaag tcaggtattc atcgacccgc atttcggtgt aaaggggaca
     9481 gctttccggc atgtagccaa tgtgccggcg cgcttcaatc gggtcttcaa acacatcgac
     9541 gccggcgatt tcaactccgc cgcgtgtcgg atgcagaaaa ccggtcagca ttcgcatggt
     9601 cgtggttttt ccggcgccgt tcgggccgag aaagccgata atttcacccc ggctgacttc
     9661 gaacgagacg tcgtcgaccg cggtatggcc cgaaaactgt ttagtcagat tggaaacctt
     9721 aatcataggt aaagcgctcc ggaaacaacc gttgcattat ctatgagaag ccgttgcagt
     9781 caagccgcgt aactggtcca acggcgtgga aaacgggaga gattattcgc cggccagccg
     9841 gtaaccgata ccgggctcat tgatgacaga tggcgattcc agtccggctt ctttcaattt
     9901 ccgccggatg tgagtgaagt gtacgcgcag atactgcgac tgttcttctg atttcggccc
     9961 ccagacttcg cgcagaatcg tgcggtgggt gacaatccgg ccggcatgtt gcgccagcac
    10021 ccgcaggagc gaaaattcaa ttgggcttaa cttcagctcc tgcccgtgta tccgcacggc
    10081 atgagatggc atatcaacct caagttttcc gaccacgata acaggggccg attctccggt
    10141 tctggcgcgc cgctggatgg cacgtaaacg cgccaagagc tcttccgtat caaacggctt
    10201 ggtcacatag tcgtctgccc cggcatccag ggcgtcgact ttgtcatccg gctcgtcgcg
    10261 gacggagagg atcagcacgg gaacagctga ccactcgcgg agtctgcgca gaacctgcgc
    10321 accgtccata tccggaagac ccagatccag aacaatcgta tccggtgaac aggtggccgc
    10381 ctcagtcagg ccggtttctc cggttgctgt ttcctgaaca cggtagccat gtccttcgag
    10441 tgcatagcgc aacattcgtc ggacagatgc ttcgtcatcg atcaccagaa tcaaggggcc
    10501 gttttttttc gtttcgtttt tcatgcttcc ggcatccggg cttctacggg aacgctgacg
    10561 gtcacttcgg cacctttatc cggacggttg caggcatcaa cttgaccatt caaggcgctg
    10621 atcaagccgc gcgtgatgga caggccgagg cccaggccgc ctgccggagc gtgcggatcg
    10681 cggtagaact tttcgaaggc gcggaccgga tcggcaaagc cggtgccgtg gtcgcggact
    10741 gttatttcca gattgccgtt attgatccgg gcgagaattt caatgtcgct gccggccggc
    10801 gtgtgaattc cggcgttccg gataatatga atcagcgcct gttgaagcag ccgagtgtcc
    10861 gtaagcacca gcaggtcagg ggtgatcaaa ttaatccgga caggacggtt catcaattct
    10921 ttccggcata agcgcacagt atcctcgatc agatcgctga tttcgcacca ttcttttttc
    10981 acgccgggct gagcggtttc cagtcgggcc gaatccacca gcagatccac catattggta
    11041 agccggtctg acgccgcttc agcctcctcc agcagctcgc cggtctgcgg tatggacttg
    11101 agagccgtca gcgccgcctt tagtgctgtc agcggtgtcc gcagttcatg cgatacatta
    11161 tccagcagca cgcggcgcag gcggtttgat gcatcaagaa cttcagagcg ctggaaagcg
    11221 gcccggaaat gatctttttc caatgccagc gcaagctggg ttgcaaaaga ggccagaaga
    11281 tcccgttccg gcagattcag ttcgcactca aaccccagcc gcagtcccaa tacacccatt
    11341 aatgaggtgc gggtttgaag aggccagaag gtgccctcag ccccaggcag tgtatcggtg
    11401 aaacgaccgg ctatccggcg gttgctgaaa gcccattcgg ccacgctgta ttccttggaa
    11461 gaaggcgtcc acaagctggc tgcatgaggt tttgcgttta gctggcgggt attcaggtcg
    11521 cgaagataca gaactgtttt tccggaaaat aacgcgttaa tctggtcgag tgcctcacag
    11581 agcccgtcat tcatatccgg attgagcgcg ctggagcggg tcagttttaa caaagcagct
    11641 gtgcttttct gccgttgccg ctctccttct tcgcgctgac gcatacgggt ggtcaggtgg
    11701 cccatgacaa gtgccaccac aatcagcagg aaaaacatca tggcatcgtg aaactgtgcg
    11761 atccggaacg taaacaacgg cggaacaaac aaaaggttcc acagaaatgc tccggccgct
    11821 gctgtaacca gaaccacccg gcggcggaga aaaagcgcgc cggttaccgt catcagaagg
    11881 taaaacagcg cgacagacag atagccaacc cagtcgcaca gcatccaccc ggcaagcgtt
    11941 acgccagcca gcagcagaag gccccagccc cattcgcgaa agtggactcc ttcacgggta
    12001 cgcgatgtgc ggacggcttc ggcggatttc tccggacgga caacatacac atcaatatcg
    12061 ccgctgcgat gaagcagttc atccaccaga ctgccgccgc tcagcaggcg catccacaga
    12121 ctctgccgcg gcttgccgac cacaatctga gttacgttct gctcacgcgc cacacgcatc
    12181 agttcaccgg ctacgcgcga gccggacacc gattcgacct cgcctcccag ccggcgggcc
    12241 agtgcaagaa actccgtaac cctttttttc tcttcttctg tcagcggatg tccatcatcc
    12301 acataaacag ccatccaggt ggcgtcgagg gcgtcggcta tgcgccgtgt ccagcgcacc
    12361 agatcacctg caaaaggact aggtcccact ccgaccagca ggcgttcccg cgccttccag
    12421 gggccggcga tgcgttgttc acgcatatgt tcacgcattc cctgatccac ccgttcggcg
    12481 gtcaggcgca gggccatttc gcgcagagcg gtcaggttgg attcacggaa aaagttttcg
    12541 ccggcggcgg cagcccggtc gcccagatag acgcggcctt cagccagccg cttacgcagc
    12601 tgttcgggtg aaatatcaat caactcaatt tcatccgccc ggtccagcac ggaatcggga
    12661 acggtttcgc ggacggtcac tcccgtaacc tgacgcacca ccggcgcgcg gctttccaca
    12721 tgctggacat tcagcgtagt atacacatca atacccgctt ccagcagttc cagcacatcg
    12781 gcgcagcgtt tcggatgccg cgatcccggt gcattggtgt gcgccagttc atcgacaaca
    12841 gccaggcgcg gtttgcgcag aagcagcccg tcgagatgca tctccttcag aacggtgccg
    12901 cgataattta tgcaagtgcg cggcagaact tccagcccct ccagcagggc ggtggtttcc
    12961 ggacgtccgt gcgtttcgac caggccgatc accacatcga cgccttccgc tttgcgctga
    13021 cgggcggcct gaagcatcgc gtaggttttt cccgcgccgg gacacatgcc gaaaaatata
    13081 cgcagacgtc cctgctgcga agcctgtctg ttcagctccg ccagcaaagc atccggatca
    13141 gggcgaaacg gcttattcat ggcgcagagt gtatccgatc cagttccatg ttcaccagca
    13201 gaacatttac atacggaacg cccggacttc cggggcgggc attctcaaga tgcttctgca
    13261 gaatatcttc aacggccgcg aacggcaggc gacgcgcctg cgccacacgc gcaacctgaa
    13321 accgggccgc ttccagcgtg atgtgcgggt caagcccgct ggcggaggcc gccagcagat
    13381 cggacggaac gggagtcttt gccggcagcg ccagcaggtc gcgccacaca tcggcgcgtt
    13441 ccttaatctg ttcctgaagt ttcccgctcg ccgggccgag gttgcttgcg cccgacggaa
    13501 gcgtactcca ttccgcagcc gacggcctcg aacgaaaata gcccggagat tcgaacttct
    13561 gtgccagcag cgcggatcca accactctgt tatcgcggta caccagactg ccctgcgccg
    13621 tttccggaca gagcagatgc gccgcccacg tgaccgccgc cggataaatg actccggtca
    13681 aaagtgtcat catcagcagc aggcggagag ccggtattaa aatctgtttc atgcgatctc
    13741 ctttcaaatc catcccagcg cagtcagcgc aaggtcgatc agtttgattc cgagaaacgg
    13801 cgcggcgagt ccgcctgcgc catagatcag cagattgcgc cgcagcagca gtgacgctcc
    13861 ggtcgcgcgg taagcgacgc cgcgcagcgc cagcgggatc agcgccacaa taatcagcgc
    13921 gttgaaaata atcgcgctga gcacagcgct ttccggcgag gccagccgca taatattcag
    13981 cgcggccaga gggccggtgg cagaaccggt tccggcgtag aggccgagaa acagcgcggg
    14041 cagaatggca aaatatttcg ccacgtcatt ggccacgctg aaggtggtca gtgcgccgcg
    14101 cgtgatgagc atctgtttgc cgatctccac aatatccaga agcttggtcg gactgctgtc
    14161 gagatccacc atgtttccgg cttcgcgggc ggtctgcgtg ccggtatgca tggcaacacc
    14221 cacgtcggcc tgcgccagag cgggcgcatc gttggtgccg tcgccggtca tggcaaccag
    14281 atgtccgtct gcctgctcgg cccggatccg gttgagtttg tcttccggcg ttgcttcggc
    14341 cagaaaatca tccacaccgg cctccgccgc aatggcggcg gccgtagcgg cgttgtctcc
    14401 ggtaatcata atcgtgcgga tgcccatacg ccgcaactgc gcaaaccgtt cgcggattcc
    14461 gcctttgacc acgtctttga gaaagatgac gccgagcaca tgactgtctt cacagaccac
    14521 cagcggagtg ccgccctggc gcgagatcgt ttcgacatga gcctccacat cggcaggcca
    14581 gattccgtcc tgctgttcca catgctgacg gatagcgccg gcggctcctt tgcggatgga
    14641 acgggcgggg tgctctgcat ccgccggaag atcgacgccg ctcatgcggg tctgggcgga
    14701 aaacggcacg aagtgtgcgt gacggtctgc cagttctcgg ccgcgcaaag cgaaaagctg
    14761 tttggccagc acaacaacgg atcggccttc cggcgtttcg tcggccagcg acgccagctg
    14821 cgccgcatcc gccagccggt ttgcatccac gccgcgcgcg ggaagaaaat caacggccat
    14881 acggttgccc agcgtgatgg ttccggtttt atccagcagc agcacatcca catcgcccgc
    14941 cgcttctacc gcccgtccgc tggtggcgag cacgttacgg cgcgacagcc ggtcaatgcc
    15001 gctgatgccg atcgcactaa gcagcccgcc gatggtggtg ggaatcaggc agaccagcag
    15061 cgatacaagc accggaagac tcagcagtcc gccggttttt acgccgcctt cccgttcggc
    15121 atagccgagg aatgcgggca gcgttacaac cgcgagcagg aaaataaagg tcagtccggc
    15181 cagcagaaca ccgagtgcct gttcgttcgg agtgcgtccg cgcacggagc cttctaccag
    15241 cgcaatcatg cggtccagaa aagtctgtcc gggctcgctg gttacgcgca ctttaatccg
    15301 gtcgctgatg acgcgcgttc ccgccgttac cgccgagcgg tcgccgccgc tttcgcggat
    15361 gaccggtgcc gattcgccgg tgatggctga ttcatccacg ctggctacac cctcaatgat
    15421 ttcaccgtct gtcggaatcg tgtcaccggc ttcgcacagc acttcgtcgc cttttttcag
    15481 ttccgccgcc gccaccgatt cttcgcgccc attgcgcagg cggcgggctt tgagcgatac
    15541 ccgcatacgg cgcagtgcgg cggcctgtgc tttgccgcgg ccttctgcca tcgcttcggc
    15601 aaagttggca aacagtacgg taaaccagag ccacagcgca atttgcagat tgaacgcgga
    15661 cggcgtgtgg gcagagacgg cgctgactgc cagtgcgccg atgaagacga cgaacatgac
    15721 gggattgcgc aactgaacgc gcggatcaaa tttgcgcagg ctgtccagca gtgcccgccg
    15781 gagcagcgaa tgatcgaaaa tggaagaacg tgttgtcatg gtttttcctt aaagagtgcg
    15841 tccggaaatc caaagcaggt ggtcggccgc tgggccgaga aagagcgctg gaagaaaggt
    15901 cagtccgccg acaatcagaa tgacagcgat cagcagcacc gcaaaggtgg ctccgtcggt
    15961 ggggaacgaa ccggacgacg gcggcgtgat tttccggacg gcgaaccgtc ctgccagtgc
    16021 cagcatggga acgatcaccg cataacgtcc gatcagcatt gcgatgccga ggccgatgtt
    16081 gaaaaatccg gtgttggcat tcagtccggc aaaggcgctg ccgttgttgc cggcggcgga
    16141 tgaccacgcg tagaggattt ccgtaaatcc gtgcggccct gtgttggcac ggctgtccag
    16201 cgtgcccgtc cagatcatcg taatgccgca gcccagcaga ataacaacgc agggtgcaag
    16261 cacggcaact gaagccagca ggatgtcgcc gcgatccagc cgcttgccca gatattcggg
    16321 cgtgcgtccc accatcagcc ccgccagaaa tacagtgaga atgacaaagg cgatgatgcc
    16381 gtagaggccg gctccgactc cgccgaaaat gacttcgccc agcatgatgt tgaacagggc
    16441 cgccattccg gccagcggcg aaaggctgct gtgcatcgta ttcacacttc cgttggaagc
    16501 agctgtggtt gccgaagccc acaggacact ggaggccaga ccgtgccgga cttctttacc
    16561 ttcccagatg ccagccgccg aacggccagc gagctgaagt tctgccgtat aagtcagcgc
    16621 aagaccggcc agaaacattg tcagcatggc ggcaaacaga cacgctccgt gacgaggatt
    16681 tctaacggtg cgtccgaacg caaacgggag cgccgcggga atcagcagaa tcgcccacag
    16741 ttgaagcagg ttggaaaaca ccgtggggtt ctcaaaagga tgcgcgctgt ttacgccgaa
    16801 gaacccgccg ccgttggttc cgagctgttt gatggcaatc tgcgacgccg ccgggccgag
    16861 aggaatcatc tgtaccgctc cttcgagcgt gtgcgcttcg acgtaggaat ggaaggtctg
    16921 caccacgccc tgaccggcca gaatcagcgc gagcacggcg gacagcggca gaagaatgta
    16981 aactgtggcg cgcgtcatat cgcaccagaa atttcccagc tcccgtgcgg agcggcttgc
    17041 cagcccgcga gccagcgcga acgccaccgc aatgccggtg gcggcactga caaagttctg
    17101 gacggtcagg cccagcattt gtgtcagata actcagcgtc gattcgccgg agtaggcctg
    17161 ccagttcgta ttggtggcaa aactgaccgc cgtgttgaat gccaacggcc aggataccgc
    17221 aggcagtccc tgcggattaa acggcagccg gttctgcaga agttgcagaa ccatcacggc
    17281 gatgaatccg gtcaggttga atgccagcag tgcgccggta taatgttgcc aatccatcgt
    17341 ctccgacgga ttaattccgg agccgcggta gatcaaacgt tccagccgtc ttaggaatcc
    17401 gggcagtctg ccgggagaac cgtccatcac atttgcgatc catcgtccga tccagggcga
    17461 tagcactgcc agtaaaacgg cgaacgcgcc gaataagaga atgtctgtcg tttgcatgcg
    17521 gacatcctgc tctgaaatac cggttgcggg tagttaagac tgggcggaaa cgcgctaaaa
    17581 aaacactaac acgcaaactt ggctactcgt agcgcagggc ttcgatcggg tcgagcttgg
    17641 aggctttcat ggcgggccag agaccgaaga aaattccgat gaagaccgaa aagccgaagg
    17701 cgacgattag cgacaggctg ctgatctgca
//
LOCUS       DAOPZO010000027        16654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_3672_length_16654_cov_9.168203, whole genome shotgun sequence.
ACCESSION   DAOPZO010000027 DAOPZO010000000
VERSION     DAOPZO010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 16654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16654
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3672_length_16654_cov_9.168203"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PKI68_03195"
     CDS             complement(<1..397)
                     /locus_tag="PKI68_03195"
                     /inference="COORDINATES: protein motif:HMM:NF014149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52731.1"
                     /translation="MIRLDRKAAILLTGVMIALGSALSAAAEVTHITLKADDTGREFE
                     GLGGNSSGGTAVFLRDYPEPYRSDILDYLFKPKYGLSLQHLKVEIGSGVDSTCGAEPS
                     VVITPDELKNPVPRGYELWLAAEARKRNPN"
     gene            734..1759
                     /gene="recA"
                     /locus_tag="PKI68_03200"
     CDS             734..1759
                     /gene="recA"
                     /locus_tag="PKI68_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNX52732.1"
                     /translation="MAAKTDKKEGASNLEAVLADIKKQYGDGAIVRLGDESGPRKIPC
                     ISTGALTLDIALGIGGVPRGRVIEIFGPESSGKTTLVSHIIANVQKEGGTAAFIDTEH
                     ALDPGYAQKIGVDLNNLLVSQPDSGEEALNICESLVKSNSIDVVVVDSVAALAPRAEL
                     DGEMGQSHVGLQARLMSQALRKLTSAISRSNCCCIFTNQIREKVGVMFGSPETTPGGR
                     ALKFYSSVRLDIRRIGQLKDTAGAIYGNRTKVKVVKNKVAPPFTTAEFDILYAEGISW
                     TGSIVDAGTDCGLIEKKGSWLSYNGQQLGQGRDGARKALMEDKKLADELAGKIKSIAK
                     AKTDGSE"
     gene            1774..2664
                     /locus_tag="PKI68_03205"
     CDS             1774..2664
                     /locus_tag="PKI68_03205"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNX52733.1"
                     /translation="MSVKSISVCVAVSCLFFSGCKKATFEGAKAMAETEALVKIGPRE
                     AGGGGARRASVLLESRLKALGLKTTIDTFSEDTPGGKVHFNNVLARIPGKTNRLIVLA
                     SHFDTKSGISKDFQGANDSGSSSGVLMELTRVLSKRGPYETEFMIAFLDGEECRKEYG
                     PHDGLHGSRRLAQQIYAAGGARLVQAVIVLDMVGDKNLNVSIPRNSSPALMKEIFYAA
                     HELGVRAQFSIGPGSILDDHVPFLLAGMPVVDVIDFDYGSVPGKNDYWHTPADTLDKL
                     SVKSMQTVGDVVLKTIENLQ"
     gene            complement(2665..3306)
                     /locus_tag="PKI68_03210"
     CDS             complement(2665..3306)
                     /locus_tag="PKI68_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52734.1"
                     /translation="MKNRDTIFILSLLSSAALCMADDEGFKRYQLILDKPPFGQAPPE
                     AEVVQVAASQSFARNLRLSMLFEGPDGTTRAGIVDNTTKKSYILRVGEPQDGLEVVEA
                     DVKTSEAMLRKGNEVALFKLEAGAGEPISKSEQSSRQSSSAERRRALLQKVEERRTQA
                     EPPPEPKLSGEALKKHLENVQMDAIRNGLPPLPLPLTPEMDAQLVKEGVLPPQ"
     gene            3388..5055
                     /locus_tag="PKI68_03215"
     CDS             3388..5055
                     /locus_tag="PKI68_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HNX52735.1"
                     /translation="MRKKSVFSLITVLLAVAVQAQMPHRVAVLVNENSQNSKKAANVF
                     AALHGVPGGNLIYLNLPESIVKGRAECTPEEFAQYIYDPAQKIIGERGLTNQVLAWVY
                     SVDFPIRIVTSPNDRQQMSLIGLTFSRGKVPALEMVEKGQFISPLFAGPVNEGGPKNG
                     TQSFDILSANLKEKMPLPSMMLGYTGENGTDMDTVLRCLQNGILARQSGFKTPVLLVQ
                     TDDKARSGPREWQYADVKTELAQRGGSAEIYTNQPPARTNLLGVLTGAENVKPADFGT
                     FAPGAFCEHLTSWSAEFQKPQTKCTEWLKAGATVTAGMVTEPYANWLKFPHARFFVHY
                     ASGCSAMESFYQSIASPVQVLLLGDPLSQVAGIPVEIKTVGLSKVITSGLDSAFVAEA
                     KFPVAAPVIYSALLDGKQIKEADNISLIELPFKEMGDGYHEVRIVAQALFPVTPGGFK
                     DFPVAINKKGRSVSIAGIANGAARQIVVKPAAAGKEKPKEIGLLWNARLLDRKPYAEG
                     AELSVDERILGEGPHRIQAVAFYEDGMEVRSTPQVFVMTYNPEVKAE"
     gene            5052..5573
                     /locus_tag="PKI68_03220"
     CDS             5052..5573
                     /locus_tag="PKI68_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNX52736.1"
                     /translation="MSLRCVFFDRDGVVNRVPDPERYVTGWDKFHLLPAFVESLRIVQ
                     QRGFVAVVVTNQQGVGKGEYSLETVQEMHRNLRRVLAEQGLELLDVYCCPHLAADNCA
                     CRKPKPGMFLQAAAKHDIDLQASWMVGDQEHDMTAGHAAGCRCIRVGGEKTAAEHQVA
                     SMNDLPALLDKIL"
     gene            5594..6040
                     /gene="tsaE"
                     /locus_tag="PKI68_03225"
     CDS             5594..6040
                     /gene="tsaE"
                     /locus_tag="PKI68_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNX52737.1"
                     /translation="MQWISNSPSDTWKIAAEFFQSLKKPAVIALHGNLGAGKTCFTQG
                     LAQAAGVKEPVCSPTYTIISEYCGTVPFHHIDLYRLGGPEEAFDLGLDEYLEAGGITV
                     IEWAERAAELLPPSTIHIRIVRGADEEQRLICVVENPLGEKMKDER"
     gene            6037..6687
                     /gene="tsaB"
                     /locus_tag="PKI68_03230"
     CDS             6037..6687
                     /gene="tsaB"
                     /locus_tag="PKI68_03230"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNX52738.1"
                     /translation="MKDESGIVLGIELSSRQGSVALLKDGALLAEKTWIGEHVRHGVL
                     FQVLENLLSEAGVAYRDISLYAAGRGPGSFSGMRMSFAVAQALALPGQTEVRAVSSGA
                     ALALAVARETGVRQIAVVGDARRGQVWYGLFETMTNGIVELADEWKLVPYEKVTVPAG
                     TLVVSPEAERLKGLFPDIGNPQFPLAQQVAELSLMQSEPLEPLYMHPPVFVEPKFG"
     gene            6720..7643
                     /locus_tag="PKI68_03235"
     CDS             6720..7643
                     /locus_tag="PKI68_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52739.1"
                     /translation="MKNKPLFFLTILSVCSLAQAVIISGVNGTGNTTGTGAPGWDYVG
                     KISSANGASSSVTYLGNSWFVTANHVKQLDNPTGVVLNATAYSIDSSSWRRITNSSGS
                     GADLMLFRVNSNVAGLSAVTIASNTPATGAALTMIGNGMNRAASSTTWFVDTTPGSWV
                     WSTSRFSGWDSTLQGYSYASGTTKRWGSNTADGSIAGIDDGFGVTDMFYTDFDNIANE
                     AQGATYDSGGGVFTGTTNNFQLAGVMLTVGTYNSQPANTAVFGDVTYVADLTAYRDQI
                     NQVIPEPATAVLLAGIGIVFGVIKRMRYMYQ"
     gene            7640..8911
                     /locus_tag="PKI68_03240"
     CDS             7640..8911
                     /locus_tag="PKI68_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HNX52740.1"
                     /translation="MKTRLISLLFFLMAGSSPAVLFYSTGDASYHTNAPTGALEGSGW
                     QFQGRFGSYLGTPISPHHFIAAQHISAGTSVGTKFYYNGSVYTTTAKTNDSASDLTIW
                     TVAERFTSYAPLYSGTSESGKGLVVFGRGVDRGVAITNTLTTITGHGQSTTTNVTVIT
                     NGWKWGSYNYTQRWGTNLVSTTATMGGYPVLVANWDAGGDDCMLADKDSSGGVFIQES
                     GVWKLAGINYSIGPSATYSFNSDGSSPFNAAILDFRGDAPLYYFNGTSWVSLPSSQKS
                     SFFVSRISSRYSWITNNVTDFDQDVDLLPDWWEKTYTNSATAMSASDDNDGDGFSNLQ
                     EYTADTNPTNAASFFEISGFLASANQTVYFTGSTGRQYQVFYTTNDLASTDLVWIAAN
                     TNTVWGFGTNSSITVTNTADKAFYRLRVTAP"
     gene            8962..10056
                     /gene="aroB"
                     /locus_tag="PKI68_03245"
     CDS             8962..10056
                     /gene="aroB"
                     /locus_tag="PKI68_03245"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNX52741.1"
                     /translation="MKYDVHVDLGDRSYPIHIGSGILDSLGEACKTAGLKGKCLVVTD
                     ESVAPLYAGRAVQSLEKAGFKPALEVLPAGETTKCQENLFRLYSRAAVHGLDRKCFMV
                     ALGGGVIGDLTGFAAASWLRGIRFVQVPTTLLSMVDSSVGGKTGINIPEGKNLVGAFH
                     QPELVLIDLDTLKTLPPREFAAGMAEVVKYGIIWDADLFKTLELTASGLADFSCGADK
                     LGAIISRCCEIKAEVVGLDEREGGLRAILNFGHTLAHAIENVAGYGEFLHGEAVSIGM
                     IYAAHLSVALKELSVKESARIEKLLAGLALPVGVTGLAWSDIRAAMTVDKKTVGGLTK
                     FVLVDRIGHAEFGCEVPEGLLEEVWHGISQ"
     gene            10040..10645
                     /locus_tag="PKI68_03250"
     CDS             10040..10645
                     /locus_tag="PKI68_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52742.1"
                     /translation="MASVNEQIVREYFETLGFFVIQPNKHQVAARHKLDSEEIDLLIA
                     RPGQAGVFTTPSEIIWGAPALAKVQRAIVSIRGWHTDRFSPAVLKKNPDIFRFAELSV
                     IKEARKTLGPGSITKILCLSDLTSAVAPQEETLTLLRAGGIDGVLSFRTMLLELMESV
                     DVSKNYEKSDLIQTLRILKTYDLLKDAQLDLFEKRKTRKAG"
     gene            10677..13136
                     /locus_tag="PKI68_03255"
     CDS             10677..13136
                     /locus_tag="PKI68_03255"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNX52743.1"
                     /translation="MRNQRIHLAATVALMAGLAAAGDAAQSRIEEQFNLGWSFIRASK
                     STLLQAPDAKAAWQPVSIPHDYSIESKFVDANEKPLGELSVEALGLHLGQRSTGYLPG
                     GPAWYKKAFKLPENCAGKRVYLLFDGVYMQSEVYLNGHKLGGHYYGYTAFQYDLTPYL
                     SFDKPNELVVRTDNELKSRWYSGSGIYRDVTLVVTDPVHIPIWGTYVTTPSVNGQNAL
                     VEMETQIRNQSAKAQTVRLVSRILDPSNTEVSRVESDSSLEPGAEKTVKQNVTVGNAR
                     LWSPEAPQLYRVVSEVIRDGKQLDEYVTPFGIRTLKFDPDEGLTVNGQPTRLKGVCIH
                     HDNGILGSEAYAWSEERKVIQLKEMGCNAIRFSHNPPSKHMLDACDKHGMLVIDEAFD
                     EWRQGKAKGYSPVFDTYWKEDMTSMLLRDRNHPSVIMWSIGNEVPDQGSEAGPAIANM
                     LSSYVKSLDPTRPTTVAVQPSGKAWGQRGKKGAPEFVSEEFFSQVDVCGYNYQNSSQK
                     IGGDFKKNHQEFPKRIMYQSESMNAIFFSDWENILSTKYVLGDFVWTGVDYLGEVGCG
                     KDLPGQEKFPSYFAMCGDLDICLFRKPRSFYRQIIWNREAIVHATVRRENQDKVNNWG
                     APLTSSSWTFDAAPGKTLAVDVYSGCEEAELFLNGKSLGRKPTGTATSLMATWDVPYE
                     KGELKAVGYVKGKAVAEHSLKTAAAVQQISLKADRPVIAANGCDVAYLTVELVDSQGV
                     RNPNAEAEITVELTGPATIAGIGTGRPEAPLAYPFHGNKSMTFQGRALLVIRSTNAPG
                     SVGVKVTSLGLKAAEATVLVQ"
     gene            13208..14326
                     /gene="dprA"
                     /locus_tag="PKI68_03260"
     CDS             13208..14326
                     /gene="dprA"
                     /locus_tag="PKI68_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNX52744.1"
                     /translation="MTDREAYIALNMIEGIGPIKVRALIDALGSPQAVFDPEQTGLRA
                     VKGIGEKLFETILTQRTQVDPQAELEKAESLGVRIITPVDAEYPPALKTIYDPPLALY
                     VRGELLPKDRHALGIVGSRQCTSYGLSTADRLAYQLAQTGFTVVSGLARGIDTVAHEG
                     ALKGKGRTIAVLGAAIDQLYPPENAELADAIAGSGAVLSEYPLGRQADRQTFPYRNRI
                     ISGLSMGVIVVEAGFKSGALHTSDAAMEQGRSVFAVPGRIDSPASKGTNRLIKNGAKL
                     VDNVNDILEEFELLIPPGQFERPEKASSARPEVPLSEDEQLLVKVLWEEALDVDSLIR
                     AAGLPSARVSALLIGLEMKRVVKMLPGRVVELAEDLRN"
     gene            14334..>16654
                     /gene="topA"
                     /locus_tag="PKI68_03265"
     CDS             14334..>16654
                     /gene="topA"
                     /locus_tag="PKI68_03265"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNX52745.1"
                     /translation="MAQKLVIVESPAKAKTINKYLGDGYIVKASMGHVRDLPPKKLGV
                     DVEKGFEPEYVNTTGRAKVITELKAAAKKCDEVILAPDPDREGEAIAWHLFELLKGIV
                     PPEKFSRVTYNEITKPAIKEAFSKPGAIDMNRVNSQQARRVLDRLVGFKVSPLLWRQI
                     KGGVSAGRVQSVALRLVCEREREIQNFTPEEYWVLGANVRKQVAPRDLFPVKLAQING
                     EKAEIKGSEQAQKIRTELEASSLHVARIIEKEIQRKARAPFITSSLQQAASSVCGFSP
                     ARAMSIAQALYENGLITYMRTDSFNIAKSAQDACFAFVAAEYGPEYLPEKPNFYKSRG
                     NAQEAHEAIRPTDVTQTPQSLSGLKPEEQKLYKLIWERFVASQMVPSRIARRTAEIET
                     GGANVYLFRATSSEIVFPGYMRASGIEKAADKKNEDDDEENEEVKLPPLTEGEALDCV
                     EWLGEQKFTKPPARYSEPSLIKALEENGVGRPSTYAQTLATLDKREYVTKEKRSLVPT
                     EAGLRVNDFLVSALDPLFNVKFTAEMEEKLDQVEHGTVEWADMMQKFYTQFLEWLEGA
                     RDMAKPEETSRVLAALEQVQEWAEPVKRGRRTYDDRETVQSVREKFAEDGQITTRQQQ
                     MLFNLACKYMSQISGEQAGELHLAKPEAASAETVRKLELLAGVKFGEARKVGKKVYDD
                     GKFCESLRQQVQGGRNLSERQLAALDSVLTKYSDQIADFETIKASLNMAAKPETADAG
                     ETGALLELLKPVQTWNPPVMRGKREFNDQSFYES"
BASE COUNT         3645 a         4518 c         5055 g         3436 t
ORIGIN      
        1 tattggggtt ccgcttgcgg gcttcagcgg cgagccacag ctcatagccg cgcgggacgg
       61 gattcttcaa ttcgtccggc gtgatcacaa cagacggctc cgccccgcag gtggagtcca
      121 ctcccgagcc gatctccacc ttcagatgct ggagactcag cccgtacttc ggcttgaaca
      181 gataatccag aatatcgctg cgatacggct ccggatagtc gcgcaggaaa acggcggttc
      241 cgccggaact attccccccc aatccttcaa actcgcgccc ggtgtcatcc gccttaagcg
      301 tgatatgtgt gacttcggcc gcggcagaga gtgccgagcc cagggcgatc ataacgcccg
      361 tcagaagaat tgctgccttg cggtctagcc tgatcataat ttccctccga gttatggata
      421 gcgttgctca gttcgttccc ggaaacagac gaaaccgccg ccggaaaaga gtacagtgta
      481 gcaggttttt gagctttgga aagttttgcg gcgggatttc cggcattgca gccgaggtcc
      541 gtgacctcgg attccggttg gttcgcgatc atagatcgcg gcttcaagaa gaggcgaaaa
      601 agtaagaatt aacaggggga agtctctgcg gcctttgctc ttctgcgagg aatgcctccc
      661 cgacaccctc catcttaaaa agacggccgc ttctgcgctt gaacaatttt ccggggcgtg
      721 taggctctcc cgcatggctg caaaaaccga taaaaaagaa ggcgcctcca atcttgaggc
      781 agtactcgct gatatcaaaa aacaatacgg cgacggcgca atcgtccgtc tcggcgacga
      841 atccggtccc cgcaaaattc cctgtatttc caccggcgcg ctgactttgg atatcgccct
      901 cggtatcggc ggcgttccgc gcggccgggt gatcgaaatt ttcggccccg aatcgtccgg
      961 taaaaccacg ctggtttccc atattatcgc caacgtccag aaagagggcg gcacggcggc
     1021 ttttattgat accgaacacg cgcttgatcc gggatatgcc cagaagatcg gtgtcgatct
     1081 gaacaatctg ctggtttcgc agcccgactc cggcgaagaa gcgctcaata tctgcgagtc
     1141 gctggtgaag agcaattcca ttgatgtcgt ggttgtggac tctgttgccg cgctggcccc
     1201 gcgggccgaa ctcgacggcg agatgggtca gtcgcatgtc ggcttgcagg cgcgtctgat
     1261 gagtcaggcg ctgcgcaaac tgacgtccgc gatcagccgc tcgaactgct gctgtatttt
     1321 caccaaccag atccgcgaaa aagtcggcgt gatgttcggc agccctgaaa cgacgcccgg
     1381 cggccgcgcg ctgaagtttt attcttccgt gcgtctcgat atccgccgca tcggccagct
     1441 caaggatacg gcgggcgcga tctacggcaa ccggaccaaa gttaaagtgg tcaaaaacaa
     1501 agtggctccg ccgttcacca cggccgagtt cgacatcctg tacgccgaag gtatttcatg
     1561 gaccggttcc attgtggacg ccggaaccga ttgcggcctg atcgaaaaga aaggctcgtg
     1621 gctttcctat aacggccagc agctgggaca gggccgcgac ggtgcgcgca aagcgctgat
     1681 ggaagataag aaacttgccg acgaactggc cggaaagatc aaatcgatcg ccaaagccaa
     1741 aaccgacggc agtgaataag tccggagttc ccgatgtctg taaaatcaat ttctgtctgt
     1801 gtagcggttt cctgtctgtt tttttccggc tgcaagaagg cgacgtttga aggcgcgaag
     1861 gcgatggccg agaccgaggc gctggtgaag atcggccccc gtgaagccgg cggcggcggg
     1921 gcgcgccgcg cttccgttct gctggaaagc aggctgaagg cgctgggtct caagacgacg
     1981 attgatacgt tttctgaaga cacaccgggc ggcaaagttc atttcaataa tgtgctggcg
     2041 cgcattcccg gcaagaccaa ccggttgatt gttctggctt cgcacttcga tacaaagagc
     2101 ggaatttcca aagattttca gggcgcaaat gattcaggtt ccagctccgg cgtactgatg
     2161 gagctgaccc gcgtactctc taaacgcgga ccgtatgaaa ccgaattcat gattgcgttt
     2221 ctcgatggcg aggaatgccg caaagaatac ggcccgcacg acgggctgca cggcagccgc
     2281 cgtctggcgc agcagattta tgcggcgggc ggagcccggc tggttcaggc ggtgatcgta
     2341 ctcgatatgg tcggcgataa gaacctgaat gtttcaattc cccgcaacag ttcgcccgcg
     2401 ctgatgaagg aaatctttta tgccgcccat gaactgggcg ttcgcgcgca gttcagcatc
     2461 ggtcccggtt ctattctgga tgaccacgtt ccgtttctgc tggcgggaat gccggtggtg
     2521 gatgtgattg atttcgacta cggctccgtg cccggaaaga atgattactg gcacacgccg
     2581 gctgatacgc tcgacaagct gtcggttaaa agtatgcaga cggtcggcga tgtggtgctg
     2641 aagacgattg agaatttgca gtaattactg cggcggcagt acaccttctt taaccagctg
     2701 ggcatccatt tccggcgtaa gcggcagagg gagcggcggc agtccgttgc ggatggcatc
     2761 catctgcaca ttttccagat gttttttcag cgcttcaccg ctcagtttcg gttccggcgg
     2821 cggttccgcc tgggtgcgcc gttcttcaac tttctgtagc agagcgcggc ggcgttcagc
     2881 agatgaggat tggcgggaag actgttcgct ttttgaaatc ggctcgccgg caccggcttc
     2941 cagtttgaaa agagccacct cgttgccttt acgcagcatg gcttcggacg ttttcacatc
     3001 tgcttcaacc acttcaagcc cgtcctgcgg ttcgccgacg cggaggatat agcttttttt
     3061 cgtggtgtta tcgacgatgc cggcccgcgt tgttccgtcc gggccttcga aaagcatgga
     3121 gaggcggagg ttgcgggcga aggactggga cgccgcgacc tgtacgactt cggcttcggg
     3181 cggagcctgg ccgaaaggcg gtttgtccag aatgagctgg tagcgtttga agccctcatc
     3241 gtccgccata cacagcgcgg cagaggacag caggctcaaa atgaaaatgg tgtcacggtt
     3301 cttcacggtt gccggattat ccggtcttgt ttaattttcg tcaagcggta ggctctccaa
     3361 gtcgtgcttt aaaaacggga gacccgcatg cgtaaaaaaa gcgttttcag tctgattacg
     3421 gttctgctgg cggtcgcggt tcaggctcag atgccacacc gtgtcgcggt gctggtgaac
     3481 gaaaattcac agaactccaa gaaggccgcc aacgtttttg ccgcgcttca cggtgtgccg
     3541 ggcggcaacc tgatttacct gaatctgcct gaatcgattg taaaggggcg cgcggagtgc
     3601 acgccggaag agttcgcgca gtacatttat gatcccgcac agaaaatcat cggcgaacgc
     3661 gggctgacca atcaggtgct ggcctgggtt tattccgtcg atttcccgat ccggatcgtt
     3721 acgtcgccga atgaccggca gcagatgtcg ctgatagggc tgaccttctc gcgcggcaag
     3781 gttccggcgc ttgagatggt cgaaaaaggg cagtttattt cgccgctgtt tgccgggccg
     3841 gtcaatgaag gcgggccgaa aaacggaacg caatcgttcg atatcctgag cgcgaatctc
     3901 aaagaaaaaa tgccgctgcc ttcgatgatg ctgggctata ccggcgaaaa cggcacggat
     3961 atggacaccg ttctgcgttg tctgcagaac gggatacttg cccggcagag cggcttcaaa
     4021 acgccggttt tgctggtgca gacggatgat aaagcccgtt ccggcccgcg cgaatggcag
     4081 tatgccgacg ttaaaaccga actggcgcag cgcggcggct ccgcggagat ttataccaac
     4141 cagcccccgg cgcgcaccaa tctgcttggc gttctgaccg gcgcggaaaa tgtgaaaccg
     4201 gcggacttcg gcacgtttgc gcccggcgca ttttgcgaac acctcaccag ctggtcggcc
     4261 gagttccaga agccgcagac caaatgcacc gagtggctca aggccggcgc gacggtgacg
     4321 gccgggatgg tgacggaacc ctatgccaac tggctcaagt tcccgcacgc ccgctttttt
     4381 gttcattatg cctccggctg ttcggcgatg gagagtttct atcaatccat tgccagcccg
     4441 gtgcaggtgc tgctgctggg tgacccgctt tcgcaggttg caggaattcc ggtcgaaata
     4501 aaaacggtcg gactgagcaa ggtgatcacc agcggtcttg attcagcatt tgttgccgag
     4561 gccaaatttc cggtcgccgc tccggttatt tacagcgcac tgctcgacgg gaagcagatc
     4621 aaagaggccg acaacatttc gctgatcgaa ctgccgttta aggaaatggg cgacgggtac
     4681 catgaagtcc gcattgtcgc gcaggcgctt tttccggtga caccgggcgg atttaaggat
     4741 ttcccggtgg cgattaataa gaagggccgg tcggtttcga ttgccggcat cgccaacggc
     4801 gcggcgcggc agatcgtcgt gaaaccggcg gcggccggaa aggaaaagcc caaagaaatc
     4861 ggcctgcttt ggaacgcgcg cctgctggac cgcaagccgt atgcggaagg tgccgaactt
     4921 tcggtcgatg aacggattct cggtgaaggc ccgcaccgta ttcaggcggt tgctttttat
     4981 gaagacggta tggaagtgcg cagcacgccg caggtttttg tgatgaccta taatccggag
     5041 gttaaagccg aatgagtctg cgctgtgttt tttttgaccg cgacggcgtg gtgaaccgcg
     5101 tgccggatcc ggaacgctat gtgaccggct gggataaatt ccacctgctg ccggcctttg
     5161 tggagtctct gcgcatcgtg cagcagcgcg ggttcgtcgc ggtggtggtc accaaccagc
     5221 agggcgtcgg caaaggcgag tattcgctgg aaacggtgca ggaaatgcac cggaatctgc
     5281 gccgcgttct ggcggagcag ggacttgagc tgctggatgt gtactgctgt ccgcatctgg
     5341 cggcggacaa ctgcgcctgc cgcaagccga agccgggcat gtttctgcag gcggcggcca
     5401 agcacgatat cgatttgcag gcctcatgga tggtcggcga ccaggagcat gatatgaccg
     5461 ccggacacgc cgccggctgc cgctgcatcc gcgttggcgg cgaaaaaacc gccgcggaac
     5521 atcaggtcgc ctcgatgaat gacctgcccg ctctgctcga caaaatcctc tgaccgcttt
     5581 attttcccgc gctatgcaat ggatttccaa cagcccgtcc gatacctgga agatcgccgc
     5641 cgagtttttt cagtccctga aaaaaccggc cgtgatcgca ctgcacggca acctgggcgc
     5701 gggtaaaacc tgcttcacgc aggggctggc gcaggcggcc ggcgtcaaag agccggtttg
     5761 cagtccgacc tatacgatca tcagcgaata ctgcggaacc gttccgtttc atcacattga
     5821 tctctaccgg ctgggcggcc ccgaagaggc gttcgacctc ggtctggacg agtatctgga
     5881 ggccggcggc attacggtga ttgaatgggc cgaacgcgcc gccgaactgc tgccgccgtc
     5941 aaccattcat atccggattg tgcgcggtgc ggacgaagag cagcgcctga tctgtgttgt
     6001 ggagaatccg ctgggagaaa agatgaagga tgaaagatga aagatgaaag cggaatcgtt
     6061 ctggggatag agctttcatc ccggcagggc agtgtggcgc ttttgaaaga cggcgcgctg
     6121 ctggccgaaa aaacatggat cggcgaacac gttcgccacg gtgtgctttt tcaggttttg
     6181 gaaaacctgc tgagcgaagc cggtgtggcg taccgtgata tttcgctcta cgcggccggc
     6241 cgcggtccgg ggtcgttttc cggcatgcgc atgtcgtttg cggtggcgca ggcgctggcc
     6301 ttgccgggtc aaacggaagt ccgcgccgtg agcagcggcg cggcgctggc gctggcggtt
     6361 gcgcgcgaaa ccggcgtccg gcagatcgcc gtcgtcggcg atgcccgccg cgggcaggtg
     6421 tggtacggac tttttgaaac catgacaaac gggatcgttg aactcgccga tgagtggaag
     6481 ctggttccgt atgaaaaagt cactgtgcct gccggaacgc tggtggtcag tccggaagcc
     6541 gaacggctga aagggctgtt cccggatatc ggaaatccgc aatttcctct ggctcaacag
     6601 gtagccgaac tgtcgttgat gcaaagtgaa ccgctggaac cgctttacat gcatccgccg
     6661 gttttcgtgg aaccgaaatt cggttgagtt tttgcggttt ttccgtagaa ttctcaacaa
     6721 tgaaaaacaa acccctgttt tttctaacga tcttgtctgt ttgttcgctg gcgcaagctg
     6781 tgattatttc cggcgttaac ggaaccggta ataccaccgg aacaggtgct cccggctggg
     6841 attatgtcgg gaagatttcc tcggcaaacg gcgcctcttc cagtgtaact tatcttggaa
     6901 attcatggtt tgttaccgcg aatcacgtga agcagctcga taatccgacc ggagtggttc
     6961 tcaatgcgac tgcatattct atagacagtt cgagctggag gcggattacg aacagctccg
     7021 gaagcggcgc agatctgatg cttttcagag tgaacagtaa cgtggccggg ctttcggcgg
     7081 tcacgatagc cagtaacacg cccgccaccg gagctgcact gacgatgatc gggaacggta
     7141 tgaaccgtgc ggcctcatca actacgtggt ttgtagatac caccccgggc agctgggtct
     7201 ggagtacaag ccggttttcc ggctgggaca gtactttgca gggatacagt tacgcgtccg
     7261 gcaccaccaa gcgctgggga agcaataccg ctgacggatc aatcgctgga attgatgatg
     7321 gattcggtgt aactgatatg ttttataccg acttcgataa catcgccaat gaagcacaag
     7381 gtgcaaccta tgattccggc ggcggggtgt ttaccggaac gaccaataat tttcaactgg
     7441 ccggcgtcat gctgacggtc ggtacctaca acagtcagcc ggccaataca gcggttttcg
     7501 gcgatgttac ctatgtggcc gatctgactg cttacagaga ccagattaat caggtcatac
     7561 cggagccggc gacagcggtt ctgcttgccg gtatcggcat tgtattcggt gtaatcaagc
     7621 gaatgcggta tatgtaccag tgaaaacccg gctgatcagc ctgttgtttt ttctgatggc
     7681 cggttcttcg ccggcggtac tcttttactc tactggcgat gcttcctatc atacgaatgc
     7741 tccgaccggt gcgcttgagg gtagtggatg gcagtttcaa ggacggtttg gaagttattt
     7801 aggaactccg attagtccgc atcattttat agcagcccag catatcagtg ccggcacaag
     7861 tgtcggcaca aaattctatt acaatggtag cgtatatacg accacggcaa agaccaatga
     7921 ttccgcaagt gatttgacca tttggactgt tgcagaaaga tttacaagtt atgctccgct
     7981 ttattcaggg acaagcgagt cggggaaggg gttggttgtt tttggacgag gggttgatcg
     8041 cggtgtcgcc attaccaata cactaacaac aattacaggg catggacagt ccacgacgac
     8101 aaatgtcact gtcattacga atggatggaa atggggctcc tataattata ctcagcggtg
     8161 gggaaccaat ttggttagca caacggcaac gatgggcgga tatccggttc ttgtggcgaa
     8221 ttgggatgct ggtggagatg actgcatgct ggcagacaag gattccagtg gcggggtttt
     8281 cattcaagag agcggtgttt ggaagttggc agggattaat tattccatcg ggccgtcagc
     8341 gacatacagt tttaattcgg atggctccag tccgtttaat gcagcaatcc ttgatttcag
     8401 aggagatgcc cctctctatt attttaacgg aactagttgg gtctctttgc cttcttcgca
     8461 aaaaagcagc ttttttgtct cccggatatc ttcccgctac agctggatta ccaataatgt
     8521 gacggacttc gatcaggatg ttgatttgct gcccgactgg tgggaaaaaa catatacgaa
     8581 cagtgcaacg gcgatgtccg cctcggacga taacgacgga gacggttttt cgaacctgca
     8641 ggaatacacg gcggatacca atccgaccaa cgcggcatct ttctttgaaa taagcgggtt
     8701 tctggcttcg gccaatcaga ccgtttattt cactggcagc acgggccggc agtatcaggt
     8761 tttctacacc accaatgatt tagcctcgac cgatcttgtc tggattgcgg ctaataccaa
     8821 tacggtctgg ggcttcggaa ccaactcgtc gattacggtg acgaatacgg cagacaaagc
     8881 cttttaccgg ctgcgagtga cggcgccttg ataaaatcac cttgacactc cgtcggcggc
     8941 tccctatcaa ccttcgcagc tatgaagtac gacgtacatg ttgatttggg ggatcgttcg
     9001 tatcccatac acatcggttc ggggattctc gactcgctgg gcgaagcctg taaaaccgcc
     9061 ggactcaaag gaaaatgtct ggttgtcacc gacgaatccg ttgcgccgct gtatgccggc
     9121 cgggccgttc agtcgctgga aaaggccggg ttcaagccgg ctctcgaagt tctgccggcg
     9181 ggcgaaacga ccaagtgcca ggaaaatctc tttcgtttat atagccgggc tgcggtgcac
     9241 ggcctcgacc gcaaatgttt catggtcgcg ctcggcggcg gtgtgatcgg cgatctgacc
     9301 ggctttgccg ccgccagctg gctgcgcggc atccgctttg tgcaggtgcc caccacgctg
     9361 ctgtccatgg tggacagctc tgtcggcgga aaaaccggca tcaacattcc ggaaggcaaa
     9421 aacctcgtcg gcgcattcca tcagccggag ctggtgctga tcgacctcga cacgcttaaa
     9481 accctgccgc cgcgcgaatt cgccgccggc atggccgaag tggtcaaata cggcatcatc
     9541 tgggacgccg atctgtttaa aactctggaa ctgactgcgt cagggcttgc tgatttttcc
     9601 tgcggggcag acaaactggg tgcgataatt tcccgctgtt gcgagatcaa agccgaagtt
     9661 gtcggcttgg acgagcgcga aggcgggttg cgcgccattc tgaattttgg acacacgctg
     9721 gcgcacgcta tcgaaaacgt ggcgggctac ggcgaatttc ttcacggcga agcggtctcc
     9781 atcggaatga tttatgcggc gcatctttcc gtcgcgctca aagaactttc cgtcaaagag
     9841 tcggcacgga ttgaaaaact gctcgccgga ctggctctgc cggtcggtgt cacggggctg
     9901 gcgtggagtg atatccgcgc cgcgatgacg gtcgataaaa aaacagtcgg cggcctgacg
     9961 aagtttgtgc tggtggatcg gatcgggcac gccgagttcg ggtgtgaagt ccccgagggg
    10021 ctgttagagg aggtctggca tggcatcagt caatgaacag attgtgcgcg agtattttga
    10081 aacgctcggc tttttcgtga tccagccgaa caagcaccag gtggccgcgc ggcataaact
    10141 ggactcggaa gaaatcgatc tgctgattgc acggcccgga caagccggcg tatttacgac
    10201 gccatcggaa atcatctggg gcgcgccggc attagctaaa gtgcagcgcg ccatcgtcag
    10261 tattcgcggc tggcataccg accggttcag tccggcggtc ctcaaaaaaa atcccgacat
    10321 cttccggttt gccgaactct cggtgatcaa agaagcccgc aaaacgctcg gaccgggttc
    10381 gatcacgaag attctctgtc tttccgatct gacgtctgcc gttgcgccgc aggaagagac
    10441 actgacgctt ttgcgcgcgg gcggcattga cggcgtgctt tcgttccgca cgatgctgct
    10501 ggaactgatg gagagcgtgg atgtttccaa gaactacgag aaatccgatc ttatccagac
    10561 cttgcgtatt ctgaaaacct acgacctgct caaagatgct caactcgacc tgttcgaaaa
    10621 acggaagacg cgcaaagccg gataacagcc agtttgaact aaagaaaagg gagacaatga
    10681 ggaaccaacg aatccactta gctgcaacgg ttgcgttgat ggcggggctg gctgcggccg
    10741 gcgacgccgc gcaaagccgc atcgaagagc agttcaatct ggggtggtcg ttcatccgtg
    10801 cctccaagtc gactcttctt caggctcccg atgcaaaggc cgcctggcag ccggtgagca
    10861 ttccgcacga ttattccatc gagtcaaagt ttgtggatgc caacgagaaa ccgctggggg
    10921 agttgagcgt cgaagcgctg gggttgcatc tggggcagcg gtcgaccggt tatctgcccg
    10981 gcggtccggc ctggtataag aaagcgttca agcttccgga aaactgcgcg ggcaagagag
    11041 tttatctgct cttcgacggg gtttacatgc agtcggaggt ctatttgaac gggcacaagc
    11101 tgggcggcca ttattatggt tacacggcgt ttcagtatga tctgacgccg tatctgagct
    11161 tcgacaagcc caacgaactg gttgtgcgta cggataatga gctgaagagc cgctggtatt
    11221 ccggttcggg aatctatcgc gacgtgacgc tggtggtgac cgatccggtc catattccga
    11281 tctggggcac gtatgtgacg accccttccg tcaacggaca gaacgcgctg gtggagatgg
    11341 agacccaaat ccgcaatcaa agcgcgaagg cccaaacggt ccgtctggtc agccggattc
    11401 tggacccgtc gaacaccgag gtgtcccgcg tggagtcgga ctcttcgctg gagccggggg
    11461 ctgaaaagac cgtgaagcag aacgtgaccg tcggcaatgc ccgcctctgg agtccggaag
    11521 cgccgcagct ttaccgggtt gtgtctgaag tgatccgcga cggcaaacag ctcgatgagt
    11581 atgtcacccc gttcggcatt cgcacgctga agttcgaccc ggatgaaggc ctgactgtca
    11641 acgggcagcc gacgcggctg aagggcgtat gcatccatca cgacaacggc attctgggct
    11701 ccgaggccta cgcctggtcg gaagagcgca aggtgatcca gctcaaggaa atgggctgca
    11761 acgcgattcg cttttctcac aatccgccgt ccaagcatat gctggacgcc tgcgacaagc
    11821 acgggatgct ggttatcgac gaagccttcg atgaatggcg ccagggcaag gccaagggct
    11881 attcgccggt cttcgatacg tactggaaag aggatatgac cagcatgctc ctgcgggatc
    11941 gcaatcaccc gtctgtcatc atgtggagca tcggcaacga agtgcccgat cagggctccg
    12001 aagccggccc ggcgatcgcc aacatgcttt cgagttatgt gaaatccctg gacccgaccc
    12061 gcccgacgac ggttgcggtt cagcccagcg gaaaagcctg gggtcagcgc ggcaaaaagg
    12121 gtgcgccgga attcgtcagc gaggagtttt tcagtcaggt cgacgtttgc ggctacaatt
    12181 accagaactc ttcccagaaa atcggcgggg acttcaaaaa gaatcatcag gaattcccga
    12241 agcgcatcat gtaccagagc gaatccatga atgcgatttt cttcagcgac tgggagaata
    12301 ttctcagcac aaaatatgtt ctgggtgact ttgtctggac gggcgtcgac tatttgggcg
    12361 aggtcggctg cggcaaagac ctgccggggc aggagaagtt cccctcctat tttgccatgt
    12421 gcggggatct cgacatctgc ctgttccgca aaccccgctc gttttatcgc cagatcatct
    12481 ggaaccgtga agccatcgtt cacgcaaccg tacgccgcga gaatcaagac aaggtgaata
    12541 actggggcgc ccccctcacg agctccagct ggacgttcga tgcggccccc ggcaaaaccc
    12601 tggcggtgga tgtctattcc ggttgcgaag aagccgaact gttcctcaac ggcaaatcgc
    12661 tggggcggaa gccgacggga accgcgacga gtctcatggc cacctgggat gtgccctatg
    12721 aaaagggcga actcaaagcc gtcggctatg tgaagggcaa agccgtggcg gaacacagcc
    12781 ttaaaacggc cgcggcggtg cagcaaatca gcctgaaagc cgaccgtccg gtcattgctg
    12841 caaacggctg tgacgtcgcc tacctcacgg tggagctggt cgattcccaa ggcgttcgca
    12901 accccaacgc cgaggcggaa atcacggttg aactcaccgg cccggccacg attgccggaa
    12961 tcggaacggg caggccggag gctcccctgg cgtatccttt tcacggaaat aaaagcatga
    13021 cgttccaggg ccgggccctg ttggtgatcc gctcgacgaa cgctccgggt tccgtgggcg
    13081 ttaaggtgac ttcgctgggc ctgaaagcag ccgaagccac cgtccttgtt cagtagggaa
    13141 cccgacggga agggagcagg cggtaggatt gttctgccgg ttggctcccg gacgaaagaa
    13201 atgaccgatg actgatcgcg aagcgtacat tgccttgaat atgattgaag gaatcgggcc
    13261 gatcaaggtc cgggcgttga tcgacgcgtt agggtccccg caggcggtgt ttgatccgga
    13321 acagaccggc ctgcgggcgg tgaagggcat cggcgaaaaa cttttcgaaa ctattctgac
    13381 gcagcgcacg caggttgatc cgcaggccga actggaaaag gccgagtcgc tgggcgtgcg
    13441 gatcatcacg ccggtcgatg cggaatatcc gccggcgctc aaaacgattt acgatccgcc
    13501 gctggcgctt tatgtgcgcg gggagcttct gccgaaagac cgccatgcgc tgggcattgt
    13561 cggctcgcgc cagtgcacaa gctacggact cagcacggcc gaccggctgg cctaccagct
    13621 tgcgcagacg ggttttacag ttgtcagcgg cctcgcccgc ggaattgata cggtggcgca
    13681 cgaaggcgct cttaaaggaa aaggccgcac gatcgcggtg ctgggcgcgg cgattgatca
    13741 gctttatccg ccggaaaacg cggagctggc cgacgcgatt gccgggagcg gggccgtgct
    13801 cagtgaatac ccgctcggac ggcaggctga ccgccagacg tttccgtacc gcaaccgcat
    13861 cattagcggt ctttccatgg gtgtcattgt ggtcgaagcc ggcttcaaga gcggggcgct
    13921 gcatacctcg gacgcggcca tggaacaggg gcggagcgtc tttgccgtac cgggccggat
    13981 cgactccccg gcatccaaag gaaccaaccg cctgataaaa aacggggcga aattggtcga
    14041 caacgtgaat gatatattag aagagttcga gctcttgatc cctccggggc agttcgaaag
    14101 accggaaaag gcttcttcgg cgcggccgga ggtgccgctc tcggaggatg aacagctgct
    14161 ggtgaaagtc ctctgggaag aggcgctgga tgtggacagt ctgatccggg cggccggact
    14221 gccgagcgcc agagtgagtg cgctgctgat cgggctcgaa atgaagcgcg tagtgaaaat
    14281 gctgccgggc cgtgtggtgg agctggcgga agatttaagg aactgaggat tttatggcac
    14341 aaaaactggt cattgtggaa tctcctgcga aggcgaaaac catcaataaa tatctgggcg
    14401 atggctacat cgtcaaagcc tcgatggggc acgtccgcga cctgcctccg aaaaagctcg
    14461 gggtggatgt cgaaaaaggc ttcgagccgg aatatgtcaa caccaccggc cgcgcgaagg
    14521 tgattaccga gctgaaggcc gccgccaaga agtgcgatga agtgattctg gcgcccgacc
    14581 cggaccgcga aggggaagcc attgcctggc atctgtttga gctgctcaaa ggcatcgtcc
    14641 cgccggaaaa attcagccgt gtgacctata acgaaatcac caagccggcg atcaaagagg
    14701 ccttttccaa gcccggcgcg attgatatga accgcgtcaa ttcgcagcag gcgcgccgtg
    14761 tgctcgaccg gctggtcggc tttaaagtca gtccgttgct gtggcggcag atcaagggcg
    14821 gcgtcagcgc cggccgcgtg cagtcggtgg cgctccgtct ggtctgcgaa cgcgaacgcg
    14881 aaattcagaa ctttacgccg gaagaatact gggtgctcgg cgcgaatgtg cgcaagcagg
    14941 tggccccgcg cgacctgttt cccgtgaagc tggcgcagat taacggcgag aaggccgaga
    15001 tcaaaggcag cgagcaggca cagaaaatcc gcaccgaact cgaagcgagt tcgctgcatg
    15061 tggcgcgcat catcgaaaaa gagattcagc gcaaggcgcg cgctccgttc attaccagtt
    15121 cgctgcagca ggccgcttcc agcgtctgcg gtttttcacc ggcgcgggcg atgagcatcg
    15181 cgcaggcgtt gtatgaaaac gggctgatca cctacatgcg aaccgactcg ttcaacatcg
    15241 ccaagagcgc gcaggacgcc tgctttgcgt ttgttgcggc ggaatacggt ccggaatatc
    15301 tgcccgaaaa gcccaatttc tataagagcc gcggcaatgc gcaggaagcg cacgaagcga
    15361 tccgcccgac cgacgtcacg cagacgccgc agagcctgag cgggctcaag ccggaagagc
    15421 agaagctcta taagctgatc tgggaacggt ttgtcgccag ccagatggtg ccttcgcgca
    15481 tcgcccgccg caccgcggaa atcgaaaccg gcggcgccaa tgtctatctg ttccgcgcca
    15541 cgtcgtcgga gattgtcttc cccggctata tgcgcgccag cggcattgaa aaagccgccg
    15601 acaagaagaa cgaggatgac gacgaggaaa acgaagaagt aaaactgccg ccgctgaccg
    15661 aaggggaagc cctcgactgc gtcgaatggc tcggcgaaca gaagttcacc aagccgccgg
    15721 cgcgctacag cgaaccgtcg ctgattaaag cgctcgaaga aaacggcgtc ggccgcccga
    15781 gcacctacgc ccagacgctg gccacgctcg acaagcgcga atacgtcacc aaagaaaagc
    15841 gctcgctggt tccgacggag gccggcctgc gtgtaaacga ctttctcgtc agcgcgctcg
    15901 atccgctgtt caatgtgaaa ttcacggccg agatggaaga gaagctcgac caggttgaac
    15961 atggcacggt cgagtgggcc gacatgatgc agaaattcta tacccagttt ctcgagtggc
    16021 tggaaggcgc ccgcgatatg gccaagcccg aggaaaccag ccgtgtgctg gcggcgctgg
    16081 agcaggttca ggaatgggcc gagccggtca agcgcggacg ccgcacctat gacgaccgcg
    16141 aaacggtgca gtcggtacgc gaaaaatttg ccgaggacgg acagatcacc acgcggcagc
    16201 agcagatgct gttcaatctg gcctgcaaat acatgagcca gatcagcggc gaacaggccg
    16261 gtgaactcca tctggcgaag ccggaagcgg cttcggccga aacggttcgt aagctggaac
    16321 tgctggcggg cgtcaagttc ggcgaagcgc gcaaagtcgg caagaaggtg tatgacgacg
    16381 gcaagttctg cgaatcgctg cgccagcagg tgcagggcgg acgcaacctc agcgaacgcc
    16441 agcttgccgc gctcgactcc gtgctgacga aatattccga tcagatcgcg gatttcgaaa
    16501 cgatcaaggc gagtctcaat atggcggcga aaccggaaac ggcggatgcc ggagaaaccg
    16561 gtgcgctgct ggagctgctc aaacccgttc agacctggaa tccgccggtc atgcgcggca
    16621 agcgcgagtt caacgaccag agcttttatg aatc
//
LOCUS       DAOPZO010000028        16370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_3761_length_16370_cov_9.225314, whole genome shotgun sequence.
ACCESSION   DAOPZO010000028 DAOPZO010000000
VERSION     DAOPZO010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 16370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16370
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3761_length_16370_cov_9.225314"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..85)
                     /locus_tag="PKI68_03270"
     tRNA            complement(1..85)
                     /locus_tag="PKI68_03270"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49..51),aa:Leu,seq:caa)
     gene            complement(159..1325)
                     /locus_tag="PKI68_03275"
     CDS             complement(159..1325)
                     /locus_tag="PKI68_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HNX52746.1"
                     /translation="MVTFEGTGIKNLKLRGGIYYWRQKIDGVQYSESLQTGDLNEARR
                     RMAEKVEAVKSGENLKEKKMTKQQDTSAGWVDYWPQLKVLDCTIRDGGLMNDHRFSDE
                     FVFGVHKALVAAGVDYMEIGYKADKKLFSPEEYGLWKSCDEDDLNRFFEGVEKGLKFS
                     VMADVGRTNESDIVPRDKSLIDLVRVACYIHQIPAALDMVKDAKDKGYETSVNLMAIS
                     TVPEYELNKGLELIGRSETDMVYLVDSFGSLYFEQIDDLMRRYLAAMGKGKQVGIHAH
                     NNQQMAYANTIFAAIHGATMLDSTIDGLGRGAGNCPTELLLGFLRNPKFKQRPVIEAV
                     EKLTLPLRDQIDWGYSIPYMISGQMNQHPRTAMALRESDRKNEFIKFYDELTAQ"
     gene            1477..2331
                     /gene="thyX"
                     /locus_tag="PKI68_03280"
     CDS             1477..2331
                     /gene="thyX"
                     /locus_tag="PKI68_03280"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNX52747.1"
                     /translation="MAHITVPEAEKLLDQEIKVLDKGFVRLVDYMGGDARIVQTARVS
                     YGEGTKTIRQDSGLIDYLLRHEHTSPFEHVIFELHCKMPIFVARQWIRHRTARLNEIS
                     GRYSVMKDEFYLPPRSQISLQSTDNKQGREKEAVPVELQNKVLELLKKDQSASFANYT
                     EILDDGIARELARINLPLSMYTEWYWQMDLKNMLHFLKLRMDSHAQWEIQEYARAIAK
                     IVKAVCPLAYESFERHILRGAKFSSDEIAAINALLAGKPNPLTDRRLAEFEDKLRGNL
                     SLFEKPAE"
     gene            complement(2350..2964)
                     /locus_tag="PKI68_03285"
     CDS             complement(2350..2964)
                     /locus_tag="PKI68_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNX52748.1"
                     /translation="MTRKVSSAYVWFDTEFSSLELDEAHLLQVAVMLTDKDLKRITPP
                     DEDLNFYIRLPDSVQLSPWVEKNIPDLIKKCRSHAAVPVEEVDAKLTAFVDRWCGTPN
                     KTVQKRPLMAGNSVHTDWLLARNFFPIFTGRLHYRLLDVSALKIQWLDLTDGEEFKKD
                     DPKTIKQYFPEADLRRLRQHDACYDIVASAAELAYYRAHMNFSV"
     gene            complement(2961..4169)
                     /locus_tag="PKI68_03290"
     CDS             complement(2961..4169)
                     /locus_tag="PKI68_03290"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNX52749.1"
                     /translation="MKILVINSGSSSIKYKLYESVGSDHDFTVMAQGGADRIGISGAS
                     VDCKKPDCEKEHQYLDLPDHKKAINAIFQMLTDPEKGVLKSLSELSGVGHRVVHGGED
                     FTGSVLIDGAVVEAIRKNSVLAPLHNPPNLMGIQAIYELYPDMPQVAVFDTAIHQTMP
                     PKAYLYGLPKEQYTVHKIRRYGFHGTSHGYVARQAAKTLKRPIEKLKIITCHLGNGGS
                     ITAFQGGKSVDTSMGLTPLAGILMGTRSGDLDPYIPLHIMETQGLSAEQVSAMMNKQG
                     GLLGLCGKRDMRDVREGCEKGYEGCIEALEIFVYSVQKYIGAYVAAMNGVDVIVFTAG
                     VGENATIVREKILANFGYLGLKLDKRANKANKTVITSAISKVKALVIHTDEEKVIASD
                     AFDIIKAKKK"
     gene            complement(4194..5171)
                     /gene="pta"
                     /locus_tag="PKI68_03295"
     CDS             complement(4194..5171)
                     /gene="pta"
                     /locus_tag="PKI68_03295"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HNX52750.1"
                     /translation="MSRSEFIEAVISKAKANQRTIVLPEGFDVRVTDAANMIAAEGIA
                     KVIVLGDDKAAARVGKGVTVVDPQTSEKLEGYAAAFYELRKSKGITLDQARETIKHVN
                     YFGMMMVQSGEADGLVSGAAHSTADTVRPALQIIRGAKKGGMVSSLFFMVSNNVPYIF
                     SDSGLVENPDADQLAQIAVQSAQTAKQFGIPTKVAMLSYSTKGSASSPLVDKVVAATQ
                     KAKQIIAAEYADQGFELDGELQGDAALVPSVGKSKAPGSPVAGQARVLIFPDLNAGNI
                     GYKLVQRLGGAEAYGPILQGLRKPVNDLSRGCSAEDIVGVVAVTVVQSL"
     gene            complement(5276..5656)
                     /gene="rplQ"
                     /locus_tag="PKI68_03300"
     CDS             complement(5276..5656)
                     /gene="rplQ"
                     /locus_tag="PKI68_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNX52751.1"
                     /translation="MRHLKKTVKLGRTGAHRDAMLANQVCSLIAERRIKTTLAKAKAT
                     RSLAEKMVTLGKAGTLAARRQALATIHQPAQVKVLFDEIAPGFAGRSGGYTRITKLGP
                     RMSDASEMVILEWVEAAAPVESEK"
     gene            complement(5678..6685)
                     /locus_tag="PKI68_03305"
     CDS             complement(5678..6685)
                     /locus_tag="PKI68_03305"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNX52752.1"
                     /translation="MPIRLGRFEMPKRVVKDEAASTATYGKFIAEPFEAGYGRTIGNS
                     MRRVLLSSLEGTAISSIKIQGAPHEFCNLPGVVEDVTDIVLNLKKVILKSYTREPQRL
                     KISVKGPGEVTAGDIQVNDSIQVINKDHHIATLDTNGKFEVELETRVGRGYCPAEGNK
                     KDEQEIGVIPIDSLFSPVSRVKYDVENCRVGRRTDYDKLVIEIWTDGRVTPDDALTMS
                     AAILRHHLDIFVSYDKDLIEFEASEKQIDIEKEDLRRKLNISVNEIELSVRAANCLNN
                     ANITTVGELAQKTEAEMLKYRNFGKKSLNEIKQKILDMGLSLGMTFDADLLKPPVKDI
                     E"
     gene            complement(6753..7115)
                     /gene="rpsK"
                     /locus_tag="PKI68_03310"
     CDS             complement(6753..7115)
                     /gene="rpsK"
                     /locus_tag="PKI68_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNX52753.1"
                     /translation="MKGAKNVPYGIAFIKTTFNNCIVSLTDMRGQVVSWSSAGRCGFK
                     GSRKSTAFAATTVAQEAARAAIAHGMNEVEVRVQGPGAGRESAVRAIQAAGLRVSAIK
                     DTTPVPHNGCRPRKQRRV"
     gene            complement(7284..7703)
                     /gene="rpsM"
                     /locus_tag="PKI68_03315"
     CDS             complement(7284..7703)
                     /gene="rpsM"
                     /locus_tag="PKI68_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNX52754.1"
                     /translation="MPRILGVDIPGKKRLEYALTYIYGLGLTTSREVIAKAGLDPAKK
                     ADELTDEDITKLTAAVREGYTIEGDLRREIGQNIRRLISINCYRGTRHRRGLPVRGQR
                     TKTNARTRKGARRTVGAVRGKEARSAAKTTAAPAAKK"
     gene            complement(7722..7838)
                     /gene="rpmJ"
                     /locus_tag="PKI68_03320"
     CDS             complement(7722..7838)
                     /gene="rpmJ"
                     /locus_tag="PKI68_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNX52755.1"
                     /translation="MKVKASVKKISADDIIVRRKGRVYVINKRNPRHKQRQG"
     gene            8231..9610
                     /locus_tag="PKI68_03325"
     CDS             8231..9610
                     /locus_tag="PKI68_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HNX52756.1"
                     /translation="MKSFCNCIIACSALFLYTGCSTVERELAYDNTIQVARRPFSGTA
                     EFGWRVKTFWTEETASVWRRCVSFPLLSLSGIPEVKPAPAPMKRGVMAKWLRRKTGPP
                     AQGTARLLLNGENFFPQFEESIRTATNRIDLKTYIFDNDDVAKQTADLLKARSHEISI
                     RILYDSFGTRLAWETDAPSLPTNYVYEAGDMVDYLKKDSDIDLRRACHTMLTSEHSKY
                     ILIDRRIAYFGGMNIGREYRYDWRDAMFELSGPVINALETSFERSWTLAGGETFTLFK
                     LPEPHLPDYSGANELYLTPTTPMKSYIYRGQLRAIEHAKQCIYLENPYLWNSSIVYQL
                     CAARKRGVDVRVTIPRDVNHNIGIAANKQTVKRLLDNGVRVFVYPGMTHVKAAVYDQW
                     ACFGSANFDDLSLHKNYELNIFTDNPEIVHEVKEELLEDGQKRSTEIHDAGEISIIDF
                     FTAGLSQYL"
     gene            complement(9582..10928)
                     /locus_tag="PKI68_03330"
     CDS             complement(9582..10928)
                     /locus_tag="PKI68_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNX52757.1"
                     /translation="MELIIIVLMLAVNALFAAYEMALASVSRTRLEVLLQSNRRGARS
                     AVRMKDRLEASLAVIQLGITLAGAIAAATGGAGVDEWLTPWFKQVFGVSAGTASFLAL
                     VSFVIPLSAVTIVAAELVPKMIGINNKEAVVLALSPAMNVVSRVFHPIVFVFEAAVKK
                     LVRVGQGGFSGERAAEDQTGLLELRAAAALARATRIIGPMEERIVMSAVQLSSRTVAD
                     AMIPAKDIATISAGASLIDALIAAHTHMHTRYPVCRDADNPQSIDGYITFKDIVTALK
                     VDPSGSGLAGIIRPISRLESNMTLAKALADMIRDRVHIALVMEGSTVLGLLTMEDIVE
                     ELVGDIRDEYDHLPVHLHSIGDGWLAGGGVTIEILKNAIGGSVFPDADVKLTLAEWAG
                     SLRDSSAQCGDVIQAGGVEVQVRKVRRNRVVEAVIRKAAEKNPSASMVTDTAKDPQ"
     assembly_gap    11018..11117
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11118..12334)
                     /gene="murJ"
                     /locus_tag="PKI68_03335"
     CDS             complement(<11118..12334)
                     /gene="murJ"
                     /locus_tag="PKI68_03335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNX52758.1"
                     /translation="MEHRRLLRSVGTVSFFTMLSRLLGLLRETVTAAAFGTSAVMSDF
                     VVAFRIPNMFRALFGEGALSSAFVPVFSAIRAKEGEPAAWRFARRVITLLGAVLIIIV
                     AVGIGITLLPPLVEAFRKGTQTLPLLRIMLPYLFFICLTALSQGVLNSFGKFALAAFT
                     PCLLNITWIAFVLFVCPRMGATPEDQIAGVAFGVLVSGAVQLAAQLPAMYRLGWRPGV
                     DLNAKDPQVTRFLGLMGPSALGQSVSQINMTINSIMARWATPWAAATMYFAERLLYFP
                     QGILATAMSTVLLPVFSNHAAVGEHEHIRSTINRALRMLLFVMIPAAVGLMALARPVT
                     EMLLGWGAFDVESVDRTAAVLRVYAPGLFFFGLAKVFVPAFYGLGDTRTPYRNGLHSV
                     GVNFSLNLIFTLTL"
     gene            complement(12340..13251)
                     /locus_tag="PKI68_03340"
     CDS             complement(12340..13251)
                     /locus_tag="PKI68_03340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNX52759.1"
                     /translation="MKKDHLYPLYSILLLFVVAPVPAMLAAPWVYRLLQNFAEEDSLL
                     DAPFYRVTSRLALLTVILLLPLACRLSGFKSRADYGLAKSSNRKFLLYLGAGLGAASM
                     LVAYLPGALLGAFAWDGDEKTICHHLCSVVKILTGGLFVGIFEEIFFRGFIFGALRKS
                     FGVAAGIVIGSLLFSIVHFMKPLDPPVTTAWNSGFLLIGRLFARAGDAFLQESCTLFC
                     MGLVLCTLYQWLKSVYLLIGLHAGWVWVMMFFRHFTDNQKTMTWLYGTSDWISRSWMG
                     SVLSIIFLAAVLLTRNRWKQLAETPNS"
     gene            13347..14384
                     /locus_tag="PKI68_03345"
     CDS             13347..14384
                     /locus_tag="PKI68_03345"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNX52760.1"
                     /translation="MIKNPGRLLAGNSKILSEAYEEFNRIPADLDLTDQPSHPPRTQQ
                     KTIAEPVSISGPGTFAGKSTTTITFEPTDREGWWLNRTDLPASLPIRVAIDNVWTTGS
                     IVSNIVLRAGGPHNYVRMVEHIISLRMGMDIDNLMIKIDSGDPPLFVRGSRDLIEALD
                     RAGRRTVNRPVKYVTVKEPVTLCWDHGQFLTLAPHQGREAVPKLIVDAAVSFPNAIGQ
                     QRIRFPVNFTNMQTGAEARTNTPYGKMLYCKTIGKIFADVRNLGYTDQNILIARKTRY
                     HNQPRLVRNGKSLEPVWHRGVLDLLAAIALIPDARFVGEITSYKAGHRLDCDLITQLY
                     LNDLLTPVGNG"
     gene            14381..15316
                     /locus_tag="PKI68_03350"
     CDS             14381..15316
                     /locus_tag="PKI68_03350"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52761.1"
                     /translation="MNPVREKTEYALLCGLIALLKLLPEGLINFCFRIFGALFFLLSF
                     RRRSMTLQNLEIAFPEKTAGERLGIAWRSFQNMALFTGDSFLLIAGKLTPEKIISRAD
                     GADLEKYRKIKASAKNGIIHLTGHLGNWELLATYGAVTGLESHVVARKGSNRLIDEKV
                     IVPLRTRHGNKVFYKENAMINTVKALKRNETVSFLIDQKIGKKEGVQVAFFGREILAV
                     ASCAVLQIRFHPVVVPTFMIKTGRNQYKLLVGDPIEWSDDGSAQEEQIQKLTQRYQTI
                     IEQTIRAYPDQWFWMHNRFKLMDARTRRRKKRREV"
     gene            complement(15322..16065)
                     /locus_tag="PKI68_03355"
     CDS             complement(15322..16065)
                     /locus_tag="PKI68_03355"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HNX52762.1"
                     /translation="MKTVSYYIEPALESQFAESGLASFEALMHRGTEKIQRFALSDGS
                     AFYLKREEGEPVWKKCVPMFLRGLRPHSGPLREVRLLQMLRSNGFLAMEPVAWGEQRL
                     LGVPLRGFLVVREVKGEELAKLFDRSNGTERQQLMKAFGRLVGRLHAKGFFHPVRLKD
                     LISTADGLVLIDRETSKPWRSMFFPQQCRDSLARAIRRMIRDGHQIGAGSACAFLRGY
                     RRGVAERWKISPCDLTAQICSALRSEMES"
     gene            complement(16078..>16370)
                     /locus_tag="PKI68_03360"
     CDS             complement(16078..>16370)
                     /locus_tag="PKI68_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52763.1"
                     /translation="SVGQAVNDGDYLAAWSEFPDILPYYARHPVVLIKDVADLKPVFE
                     RLADGRNIYLVIPQKDLPMLARLFEFSQLTTKENQRKPERTLAFVKILNDRR"
BASE COUNT         3804 a         4791 c         4184 g         3491 t
ORIGIN      
        1 tggtgccgga gaagggactc gaacccttac gatattactc ggcagatttt gagtctgctg
       61 cgtctaccaa tttcgccact ccggctcaaa agaagggggg aagatagaaa aagcccctgt
      121 cttttgcaag caggggcttt cacattcgat cagatgatct actgagctgt cagttcgtca
      181 tagaatttga tgaattcgtt tttcctgtcg ctctcacgca gggccattgc cgtgcgcgga
      241 tgctggttca tctgcccgct gatcatgtac gggatgctat agccccagtc gatctgatcg
      301 cgcagcggaa gcgtcagctt ctcaacagct tcaatgaccg ggcgctgttt gaacttcgga
      361 ttgcgcagga agccgagcag cagctccgtc gggcagttgc ccgctccgcg acccagtccg
      421 tcgatcgttg agtcgagcat cgtggcgccg tgaatggcgg caaagatggt gttggcatac
      481 gccatctgct gattgttgtg agcgtgaata ccgacctgct tacccttgcc catcgcggcg
      541 aggtagcggc gcatgaggtc atcaatctgc tcgaaataga gcgaaccgaa gctgtcgaca
      601 aggtaaacca tgtcggtttc acttctaccg atcagctcaa gccccttgtt cagttcatac
      661 tcgggcacag tggaaatcgc catgaggttg acggacgttt catagccttt atctttggcg
      721 tccttgacca tatccagtgc ggccggaatc tggtggatgt agcaggcaac gcgcacgaga
      781 tcaatcaggc tcttgtcgcg gggaacgatg tcactctcgt tcgtacggcc gacatcggcc
      841 attacactga acttaagccc cttttcaacg ccttcgaaaa agcggttcaa atcatcttca
      901 tcgcatgatt tccacaatcc gtactcttcc ggactgaaaa gttttttgtc agctttataa
      961 ccgatttcca tatagtccac gccggcggcg accaatgctt tatggactcc gaatacgaat
     1021 tcatcgctga agcggtggtc gttcatcaac ccgccgtcac gaatggtaca gtcgagcacc
     1081 ttcaactgcg gccagtaatc cacccaccct gcacttgtat cctgctgttt cgtcattttc
     1141 ttctccttta aattctctcc gcttttaacg gcttctacct tttcagccat ccgcctgcgt
     1201 gcttcattga gatcaccggt ttgaagtgat tcggaatact gaacaccatc gatcttctgc
     1261 cgccagtagt aaattccacc gcgcaacttc agatttttta ttccggtacc ttcaaatgta
     1321 accatgtcgc atcaccaaag tgaacaattt attattttta tgatgcgcat atgatgcgct
     1381 tttcattttg ataatcaacc ttacattcca aattgtttga atcaaaatcc tcattacctc
     1441 atcatccgcc gttcgaagat cacatggagg ttgtaaatgg cgcatatcac cgttccggaa
     1501 gctgaaaaac tgctcgatca ggaaatcaaa gtcctggata aaggatttgt ccggctggtg
     1561 gattatatgg gcggcgacgc ccgcatcgtc caaaccgccc gcgtctccta cggagaaggc
     1621 accaaaacaa tccggcagga ctccggcctt atcgattacc tgctgcgtca cgaacacact
     1681 tcaccgttcg aacacgtcat cttcgaactc cactgcaaaa tgccgatctt cgtcgcccgc
     1741 cagtggatac gccaccgcac cgcgcgcctg aacgaaatct ccgggcgcta cagcgtgatg
     1801 aaagatgaat tctatcttcc gccgcgcagc cagatcagcc tgcaaagcac cgacaacaaa
     1861 caggggcgtg agaaagaggc tgttccggtt gaactgcaga acaaagtgct cgaactgctc
     1921 aaaaaagatc agtcggccag ctttgccaac tatacggaga tcctcgacga cggcatcgcc
     1981 cgcgagctgg cgcgcatcaa cctgccgctc tcgatgtaca ccgagtggta ctggcagatg
     2041 gatctgaaaa atatgctgca ctttttaaaa ctgcgcatgg acagccacgc gcagtgggaa
     2101 attcaggaat acgcccgcgc catcgccaaa atcgtcaaag cggtctgccc tcttgcctat
     2161 gaatcctttg aacggcacat cctgcgcggc gcaaaattct ccagcgacga aattgccgcc
     2221 atcaacgcac tgcttgccgg gaagccgaat ccgctcacgg atcgccgtct cgcggaattc
     2281 gaagataagc tccgcggcaa cctttctctt tttgaaaaac cggcggaata gcttccgggc
     2341 gtccggaaac tacacagaaa aattcatgtg tgcgcggtag taagccagct cggccgccga
     2401 agcgacgatg tcatagcagg cgtcatgctg acgcagacgg cgcaggtcgg cttccggaaa
     2461 atattgtttg atcgttttcg ggtcgtcttt tttgaattct tcgccgtccg tcagatcaag
     2521 ccattgaatt ttaagtgccg agacatccag cagacggtaa tggagccgcc cggtaaagat
     2581 cggaaaaaaa ttgcgggcaa gcagccagtc tgtatgaaca ctgttaccgg ccataagcgg
     2641 ccttttctga accgtcttat tcggcgttcc gcaccagcgg tcgacaaagg ctgtcagttt
     2701 cgcatccact tcttcaaccg gaacagcagc atgcgaccgg cactttttga tcagatccgg
     2761 aatattcttc tccacccacg gactgagctg aacactgtcc ggcagacgga tatagaaatt
     2821 caaatcctcg tccggcggcg tgatccgctt gaggtcttta tccgtcagca tgaccgcaac
     2881 ctgcagcaga tgcgcctcgt cgagttcgag cgaactgaac tcggtatcga accagacgta
     2941 ggctgatgac actttccggg tcatttcttt ttcgctttga taatgtcgaa ggcgtccgaa
     3001 gcgatcacct tctcttcgtc ggtatgaatg accagcgctt tgacttttga aatcgccgag
     3061 gtaatcaccg tcttattggc cttgttggcg cgcttgtcga gcttgaggcc gagatagccg
     3121 aagttcgcca gaattttttc gcggacgatc gtcgcattct cgccgacgcc ggccgtgaac
     3181 acaatgacgt ccaccccgtt catggcggcg acgtatgcgc cgatgtattt ctgaacggaa
     3241 tagacaaaaa tttcgagcgc ttcgatgcag ccttcgtagc ctttctcaca gccttcccgc
     3301 acgtcgcgca tatcgcgttt gccgcaaaga ccgagaagtc cgccctgctt attcatcatc
     3361 gcgctgacct gctcggcgct gagcccctga gtttccatga tgtgcagcgg aatataggga
     3421 tcgaggtcgc cggaacgcgt acccatcaga ataccggcca gcggcgtgag ccccatggag
     3481 gtgtccacgg acttgccgcc ctggaacgcc gtgatcgaac cgccgttgcc gagatggcag
     3541 gtgatgattt tcagtttttc aatcggacgc ttcagcgtct tggcggcctg cctggcgaca
     3601 tagccgtgcg aagttccatg gaatccatac cggcggattt tatggacggt gtactgctcc
     3661 ttcggcaaac cgtacagata ggctttcggc ggcatcgtct gatgaatggc ggtatcgaac
     3721 accgccacct gcggcatatc cggatagagc tcgtaaatgg cctggatacc catcaggttc
     3781 ggcgggttgt gcagaggtgc cagcaccgaa tttttgcgga tcgcttccac gaccgcgccg
     3841 tcgatcagca ccgaaccggt gaaatcctcg ccgccatgca ccacgcggtg cccgacgccg
     3901 gagagctcgc tcagactttt cagaacgcct ttttccggat cggtcagcat ttgaaaaatg
     3961 gcattgatcg ccttcttgtg atccggaaga tccagatatt gatgttcttt ttcgcaatca
     4021 ggcttcttac aatccacact ggcgccggaa ataccgatac ggtcggcacc gccctgcgcc
     4081 atcaccgtaa agtcatggtc gctgccgacc gactcataga gtttgtattt gatcgacgat
     4141 gaacctgaat taataaccag aatcttcatt acactaccct gcctgttcct taattaaagt
     4201 gactgtacaa ctgtgaccgc gacgacaccg acaatatcct ccgcactgca tccgcgggaa
     4261 agatcattca ccggcttccg caacccctga aggatcggcc cgtacgcttc cgcgccgcca
     4321 agacgctgca ccagcttata cccgatattg cctgcattga ggtcggggaa aatcagaacg
     4381 cgcgcctgac cggcaaccgg acttcccggc gctttggatt tccctaccga aggaacgagt
     4441 gcggcatcgc cctgaagctc cccgtccagc tcaaatccct gatcggcata ttctgccgca
     4501 atgatctgct tcgccttctg cgttgccgcc acaactttat ctaccagcgg actggaggcg
     4561 gaaccttttg tggaatagga aagcatagcg acctttgtcg gaataccgaa ctgcttcgcg
     4621 gtctgcgcgc tctgcaccgc aatctgtgcc agctgatccg cgtccggatt ttcaaccagc
     4681 ccgctgtcgg agaaaatgta aggaacatta ttactgacca tgaaaaacag cgaagaaacc
     4741 attccgcctt ttttcgctcc gcgaataatc tgaagcgccg gacgaaccgt atcggcggtt
     4801 gagtgcgcgg caccggaaac cagtccgtca gcctcgccgc tctgcaccat catcatgccg
     4861 aagtagttaa cgtgcttgat tgtttcacgt gcctggtcga gcgtaattcc tttgctttta
     4921 cgtaattcat aaaaagcggc cgcatagcct tcaagttttt cggaggtttg cggatcaaca
     4981 actgtgacgc ccttgccaac gcgggcggcg gctttgtcat cgcccagaac aatcactttg
     5041 gcaatgcctt cagcggcaat catattcgcc gcatcggtaa cacgcacatc aaacccttcg
     5101 ggcagaacaa tcgtccgctg attggctttg gctttgctga ttacagcctc aataaactca
     5161 ctgcgactca tttttcggaa tcctttccag attaataaac tcaaaacaaa aaactaaaaa
     5221 gcctcctaag ctgttgctta agaggctttt ttcatggcga ttctcctgct gggacttact
     5281 tttcgctttc caccggtgca gcagcttcca cccattccag aataaccatt tcggacgcat
     5341 cgctcatccg cgggccgagc ttggtaatcc gtgtatagcc gccggagcga ccggcaaacc
     5401 cgggagcaat ttcatcaaac agcactttaa cctgtgccgg ctgatgaatc gtggcaagag
     5461 cctgacggcg ggcggccagc gtaccggcct tcccgagcgt taccattttt tcggcaagcg
     5521 aacgggtcgc cttggcttta gccagcgtag ttttgatgcg gcgttcggca atcaggctgc
     5581 aaacctgatt ggcaagcatt gcatcgcgat gcgcgccggt acggccgagt ttaactgttt
     5641 tcttaagatg tctcatgatg gaacctcgtt ttctaaacta ttcaatatct ttgaccggcg
     5701 gcttcagcag atcggcatca aacgtcatgc cgagcgacag gcccatatcc agaatcttct
     5761 gcttgatctc attcagcgac ttcttaccga agttgcggta cttgagcatt tcagcttccg
     5821 tcttctgcgc cagttcacca accgttgtga tgttggcgtt gttgaggcag ttggcagcac
     5881 gaacgctgag ttcaatttcg ttaacgctga tattcagctt gcggcgcaga tcttcttttt
     5941 cgatgtcgat ctgtttttcg ctggcttcga actcaatcag atctttgtca tagctgacga
     6001 agatgtcgag gtggtgacgg aggatcgcgg cagacatcgt cagcgcatcg tcaggcgtca
     6061 cgcggccatc ggtccagatt tcgatgacaa gtttatcgta gtcggtacgg cggccgacgc
     6121 ggcagttttc gacatcgtac ttcacacgcg aaaccgggga gaaaagagag tcgatcggaa
     6181 taacaccgat ttcctgctca tccttcttat taccttcagc cgggcagtaa ccgcgaccga
     6241 cacgggtttc gagctcgact tcaaactttc cgttggtatc gagagtagca atgtgatgat
     6301 ccttattgat cacctgaatg ctgtcattga cctgaatatc accagccgtc acttcgcccg
     6361 gacctttgac gctgattttc agacgctgag gttcacgggt gtaggactta agaataacct
     6421 ttttcaggtt aagaacgata tcggtcacat cttcaacgac acccggcaga ttgcagaatt
     6481 cgtgcggagc gccctgaatt ttaatggacg aaatcgcggt gccttccagc gaagaaagca
     6541 gtacgcggcg catcgagtta ccgattgtgc ggccatagcc ggcttcaaac ggttcagcga
     6601 tgaatttgcc gtaggtggca gtggatgcgg cttcgtcttt gacgacccgc ttgggcattt
     6661 cgaaacgacc tagtctgatt ggcattgtat tctcctccca gaaccgggca ttaaccggtt
     6721 catatggtgt ttatgtactt aatcccgtcc gtttaaacgc gacgctgttt tctcgggcgg
     6781 caaccgttgt gcggaaccgg agtggtatct ttaatagcgg aaacacgaag accggcagcc
     6841 tgaatcgcgc gcacagccga ctcgcggcct gcacccggcc cctgcacccg gacttccact
     6901 tcgttcattc cgtgggcaat cgcggcgcgt gcagcttcct gcgccacggt agtggcggca
     6961 aaagcggtgc tcttacggga acctttgaac ccgcaacgcc cggcgctgct ccaggaaaca
     7021 acctgacctc tcatgtcggt gagcgacacg atgcagttat tgaaagtggt cttgatgaaa
     7081 gcaatgccgt acggcacgtt cttggcgcct ttcatgcggc gtttcggttt gccgtcgtcc
     7141 gccgcaggag cctcttcaat ctgcgcaaaa atatctttct gctttgcaga cttcttggtt
     7201 tcttcggcag cggcttccac cacttcgaca acttcttcta caggtttggt ttcttcagcc
     7261 atgaatcgga tccttagtta aatttacttc ttagcagccg gcgcagctgt cgtcttggcc
     7321 gccgaacggg cttctttacc gcgaactgca ccgactgtgc ggcgggcgcc tttacgggtg
     7381 cgggcattgg tcttcgtgcg ctgaccgcgt accggcaggc cgcggcggtg gcgggtgccg
     7441 cggtagcagt taatggaaat cagacggcgg atgttctgtc cgatttcacg gcgaagatcg
     7501 ccttcgatgg tataaccttc gcgcaccgct gcggtgagct tggtaatatc ttcgtcggtc
     7561 agttcgtcgg cttttttagc cggatcgaga ccggctttag cgatcacttc gcgggaagtg
     7621 gtcaggccga gaccgtagat ataagttaat gcgtactcga gacgcttttt tccgggaata
     7681 tcaacaccaa gtatgcgagg cataatgaaa ctgctccttc tttaaccctg acgctgtttg
     7741 tgacgcgggt tgcgtttgtt aattacataa acgcgccctt tacgacggac aataatatcg
     7801 tcagcgctga ttttcttaac cgatgcctta accttcatag ttcacctaaa taataatctg
     7861 ccccacactc atatcgtaag gcgacattgc caccttcacc aaatcgccac acaccggaaa
     7921 ctttttctcc cggtcccgca ccgcaatata ggctgtgaac cggtgtccat tccctattac
     7981 ggcgcgaaaa gcgtgactat ctatcacggc ctccaactga gcgtccagta aaatagtttc
     8041 gcactcgctc atttttaaga gcggcccatc ataccgaccc gccttaccgg tacaagctcc
     8101 aattccagaa aaaataaacc ttcccggatg cggcgaaaat caggcaggtt cccgccctga
     8161 cagaccggaa tccgtccttc ccgttttcca aacgttggaa agtcaggaaa agcctgttac
     8221 tgtcccgcga gtgaaatcct tttgcaactg tattatcgcc tgctccgccc tgttcttata
     8281 cacaggctgc tccaccgtcg aacgggaact tgcctatgac aacacgatac aggtcgccag
     8341 gcgcccgttc agcggaaccg ccgaattcgg ctggcgcgtc aaaaccttct ggaccgaaga
     8401 aaccgcctcc gtctggcggc gatgtgtcag tttcccgctg ctcagtctga gcggcattcc
     8461 ggaagtaaag ccggctccgg ctccgatgaa gcgcggggta atggccaaat ggctccgcag
     8521 gaaaaccggt ccgcccgcac agggaaccgc ccgcctgctg ctcaacggtg aaaacttttt
     8581 cccacagttt gaagaatcca tccgcaccgc gaccaaccgc attgatctga aaacctacat
     8641 cttcgacaac gacgacgtcg ccaagcaaac cgccgatctg ctcaaggccc gcagtcacga
     8701 aatcagcatc cggattctct acgactcatt cggaactcgc ctcgcctggg aaaccgatgc
     8761 gccgtcgctg ccgaccaact atgtgtatga ggcaggcgac atggtcgact atctcaaaaa
     8821 agattccgac atcgacctgc gccgcgcctg ccacacgatg ctcacctccg aacattccaa
     8881 atatattctc atcgaccgcc ggatcgccta tttcggcggc atgaacatcg gccgcgaata
     8941 ccgctatgat tggcgcgacg ccatgttcga actctccggc ccggtcatca acgcgctcga
     9001 aaccagcttt gagcgatcat ggacgcttgc cggcggcgaa accttcacgc tgttcaagct
     9061 gccggaaccg catctgccgg actattccgg agccaatgaa ctgtatttga caccgaccac
     9121 accgatgaag tcctacatct acagaggaca gctgcgagcc atcgaacacg caaaacaatg
     9181 catctacctt gaaaacccgt acctgtggaa ttcatccatc gtttaccagc tctgcgcggc
     9241 acgcaaacgc ggcgtcgacg tccgcgtcac cattccgcgc gacgtcaacc acaacattgg
     9301 aatcgccgcg aataagcaga ccgtcaaacg gctgctcgac aacggcgtgc gcgtttttgt
     9361 ttatccggga atgacgcacg tcaaagccgc cgtctacgac cagtgggcct gcttcggctc
     9421 ggcgaacttc gatgacttaa gtctgcataa aaactacgaa ctgaacatct tcaccgacaa
     9481 tccggagatt gttcacgaag tcaaagaaga gctgcttgaa gacggacaga agcgttcaac
     9541 cgaaattcac gacgccggcg aaatcagcat catcgacttc ttcactgcgg gtctttcgca
     9601 gtatctgtaa ccatcgaagc ggacggattc ttttcggccg ccttgcggat cacagcctcc
     9661 acaacacggt tccgccggac tttacgaacc tgcacctcga caccgccggc ctgaatcacg
     9721 tcaccgcact gcgcggagct gtcccgcaga ctgcccgccc actcggccag cgtaagcttc
     9781 acatctgcat ccggaaaaac agacccgccg atcgcgttct tcagaatctc aatcgtcacg
     9841 ccgccgccgg caagccatcc gtcgccgatc gaatgcagat ggacaggcaa atggtcatat
     9901 tcgtcgcgaa tgtcaccgac gagctcttcg acaatgtcct ccattgtaag cagaccaaga
     9961 accgtcgagc cctccataac cagcgcgatg tgaacccggt cgcgaatcat atccgccagc
    10021 gctttcgcca gcgtcatgtt cgactcaagg cgcgatatgg gccggatgat tcctgcgaga
    10081 ccggaccccg acggatcaac tttcagcgcc gtcacgatat ctttaaaggt gatatagccg
    10141 tcaatactct gcggattatc tgcgtctctg caaaccggat agcgcgtgtg catgtgcgta
    10201 tgcgccgcga tcagcgcatc gatcagactc gcgccggcgg aaatcgtcgc gatatccttc
    10261 gcgggaatca ttgcatccgc caccgtgcgg gacgacagct gaaccgccga cataacaata
    10321 cgctcttcca tcggtccgat gatgcgcgtt gcccgcgcca gagccgccgc ggcccgcagc
    10381 tccagaagcc ccgtctggtc ttccgccgcc cgctcgcccg aaaatccgcc ctgcccgacc
    10441 cgaaccagct ttttaacagc cgcttcgaaa acaaaaacga tcggatgaaa cacgcgggaa
    10501 accacattca tcgccgggga aagcgccagc accaccgctt ccttattatt gattccgatc
    10561 atcttcggaa ccagttccgc cgcgacaatc gtcaccgcgc tcagcggaat cacaaaactc
    10621 accaaggcca gaaatgaggc ggttcccgca gacacaccga acacctgctt aaaccacggc
    10681 gtcagccact catccacgcc cgctccgccg gtcgccgccg caattgcccc ggccagcgta
    10741 atgcccagct gaattaccgc cagactcgcc tccagccggt ctttcatccg aaccgcgctc
    10801 cgcgcccccc tccggttcga ctgcaataaa acttccagcc gcgtccgcga cacagaggcc
    10861 aaagccatct catacgccgc gaataacgcg tttacggcca gcatcagtac aataataatc
    10921 agttccataa aattccctta gttcatcctt tccggcgcag gatggcgtta aaaacctggg
    10981 cggcttcgcg gcggcagagc aggacggagg cgagcagnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnagg gtcagcgtga aaatcaggtt gagcgaaaag ttgacgccga
    11161 cgctgtgcag tccgttgcgg tacggcgtac gcgtatcgcc cagtccgtag aaggccggaa
    11221 caaatacttt agccagcccg aaaaagaaaa gtcccggcgc ataaacgcgc aggaccgccg
    11281 cggtgcgatc gaccgactca acgtcgaagg caccccaccc caaaagcatt tcagtgactg
    11341 gacgggcgag cgccatcagc ccgaccgccg ccggaatcat gacgaacagc agcatgcgca
    11401 acgcgcggtt gatggttgag cgaatatgtt cgtgttcgcc gacggcggca tggttggaaa
    11461 agaccggcag gagcacggtg ctcatggcgg tggcaagaat gccttgcggg aagtagagca
    11521 ggcgttcggc aaagtacatg gtggccgcgg cccacggcgt cgcccagcgc gccatgatgc
    11581 tgttgatggt catgttgatc tgcgatacgg actgtccgag cgccgacggt cccatgagtc
    11641 cgaggaagcg ggtgacttgc ggatctttcg cgttcagatc aactcccggc cgccagccga
    11701 gccggtacat ggcgggaagc tgcgccgcca gctgcacggc gcctgaaacc agtacgccga
    11761 acgcaacccc ggcgatctgg tcttccggtg tcgcgcccat ccgcgggcag acaaacagga
    11821 cgaacgcgat ccaggtgatg ttcagcagac agggcgtaaa agccgccagc gcgaattttc
    11881 cgaagctgtt gaggacgccc tgcgacagcg ccgtcaggca gatgaagaag aggtacggaa
    11941 gcatgatgcg caaaagcggc agagtctggg ttcctttgcg gaacgcttcg acaagcggcg
    12001 gcagcaacgt gatgccgata ccaaccgcga caatgataat caataccgcg ccgagcagcg
    12061 tgatgacgcg gcgggcaaag cgccaggccg ccggttcgcc ttcttttgcg cggatcgctg
    12121 aaaagaccgg aacaaaagcg gaggagagcg cgccttcacc gaacagcgcg cgaaacatgt
    12181 tcgggatgcg gaaggcgacc acgaagtcgg acatgaccgc gctggttccg aaggcggccg
    12241 cggtgacggt ttcgcgcaac agacccagca gacggctcag catcgtgaag aagctgaccg
    12301 ttccgacgga acgaagtaat ctgcggtgtt ccataaaatt caactgttcg gggtttcagc
    12361 cagctgtttc cagcggttgc gggtcagcag tacagcagcc agaaaaatga tggataagac
    12421 ggagcccatc caggacctgg aaatccagtc gctggttcca tagagccacg tcatggtttt
    12481 ctggttatcg gtgaagtggc ggaaaaacat cattacccag acccagcctg cgtgcaggcc
    12541 gatgaggagg taaacggatt tgagccactg gtaaagagtg caaagcacaa gtcccatgca
    12601 gaaaagcgta caggactcct gcaggaaggc gtcgccggca cgggcaaaga gacgtccgat
    12661 cagcagaaac ccggagttcc acgccgtggt gacgggcgga tcgaggggct tcatgaagtg
    12721 gacgatggaa aaaagcaaac ttccgatcac gataccggcc gccacaccga aactcttgcg
    12781 caaggctccg aaaatgaatc cgcggaaaaa gatttcttcg aatataccga caaaaaggcc
    12841 gccggtcaga attttgacga cgctgcaaag atgatggcag atggtttttt catcgccgtc
    12901 ccaggcaaaa gctcccagca gcgcaccggg cagataggcg acaagcatgc tggccgcgcc
    12961 caggccggca ccgagataaa gcaggaattt acgattggag gactttgcga gtccgtagtc
    13021 ggcccgggat ttaaatccgc tgaggcggca ggcgagcggc aggagcagaa tcaccgtcag
    13081 caaggcaagg cgggaggtaa cgcggtaaaa cggagcatcg agcagggaat cttcttcggc
    13141 aaaattctga agaagccggt aaacccacgg agccgccagc atcgccggca caggcgcaac
    13201 cacaaaaaga agaaggatgg aataaagcgg atacaaatga tcttttttca tgctcactat
    13261 ttttcaccgt ttggaactcg caacactata gcggctttcg tattttagac cacgaaattt
    13321 cagatctaac cacggattac aggcatatga taaaaaaccc gggccgcctg ctggcgggca
    13381 acagcaaaat actttccgag gcgtatgagg agttcaaccg gattccggca gatcttgatc
    13441 tgacggatca gccgagccac cctccccgca cccagcagaa aaccattgct gaaccggtga
    13501 gtatttccgg gccgggcact tttgcgggaa aatccacaac gacgattacg ttcgagccga
    13561 ccgaccgcga aggctggtgg ctgaaccgga ccgatctgcc cgcttccctg ccgatccgcg
    13621 tagcgatcga caacgtctgg acgaccgggt cgatcgtcag caacatcgtg ctgcgcgccg
    13681 gcggcccgca taactatgtg cgtatggttg aacacattat ctctctgcgc atgggcatgg
    13741 atatcgacaa cctgatgatc aaaatcgatt ccggcgatcc gccgctcttt gtgcgcggaa
    13801 gccgcgatct gattgaagca ctggaccggg ccgggcggcg gacggtaaat cgtccggtga
    13861 aatatgtaac ggtaaaagag ccggtcaccc tctgctggga tcacgggcag tttctgactc
    13921 tggctccgca tcaaggcaga gaggccgttc cgaagctgat tgtcgatgcc gcagtcagct
    13981 tccccaacgc catcggccag cagcgcatcc ggttcccggt taactttacc aacatgcaga
    14041 ccggcgccga ggcgcgcacc aacacgccgt acggaaaaat gctctactgc aaaaccatcg
    14101 gcaaaatttt cgcggacgtc cgcaatctcg gctacaccga tcagaacatt ctgattgcgc
    14161 gtaaaacgcg ttatcacaac cagccgcgcc tcgtgcgtaa cggcaaatcg ctggaaccgg
    14221 tctggcaccg cggtgttctc gacctgctgg cggccatcgc actgattcct gatgcgcgct
    14281 ttgtcggtga aatcacctcc tacaaagccg gccaccgcct cgactgcgat ctgatcacac
    14341 agctttacct gaacgatctg ctgaccccgg ttggaaacgg atgaatcctg tcagggaaaa
    14401 aacggaatat gcgctgcttt gcgggctcat cgctctgctg aaactcctgc cggaaggact
    14461 gattaacttt tgtttccgta ttttcggagc gctctttttc ctgctcagtt tccggcggcg
    14521 ctccatgaca cttcaaaacc tggaaatcgc ctttccggaa aagacggccg gcgaacggct
    14581 cggtatcgcc tggcgctctt tccagaacat ggcgctgttt accggcgaca gctttctgct
    14641 gatcgccggc aagctgacgc cggaaaaaat tatttccaga gcggacggag ccgaccttga
    14701 gaaataccgg aagatcaaag cctccgccaa aaacggcatc attcatttga ccggacatct
    14761 cggaaactgg gaactgctgg caacctacgg cgccgtaacc ggactggaat cacatgtcgt
    14821 cgcccggaaa ggctcaaacc ggctgattga tgaaaaagtc attgttccgc tgagaacccg
    14881 ccacggcaac aaggtctttt acaaagaaaa cgccatgatc aataccgtca aggcgctgaa
    14941 acgcaacgag accgtttcct ttttgatcga ccagaaaatc gggaaaaaag aaggtgttca
    15001 ggttgcgttt ttcggccgcg aaatactggc cgtcgcgtcc tgcgccgttc tccaaatccg
    15061 gtttcatccc gtcgtggttc caacgttcat gatcaaaacc ggacgcaatc aatacaaact
    15121 gctggtcggc gacccgattg aatggagcga tgacggctca gcgcaggaag aacagattca
    15181 aaagctgacc cagcgctatc aaacgatcat tgaacaaacc atccgcgcct atcccgacca
    15241 gtggttctgg atgcacaacc gctttaagct catggacgcc cgcacccgcc gccgcaaaaa
    15301 acgccgcgag gtctgaatga atcaggactc catctcggag cgcaacgccg aacagatctg
    15361 agccgtcagg tcacaaggtg aaatcttcca acgttcggcc actccgcgcc ggtagccgcg
    15421 caaaaacgcg caggcgctgc cggctccgat ttgatgtccg tcacgaatca tccggcggat
    15481 ggcgcgggcc agcgaatcgc ggcactgctg cggaaagaac atcgagcgcc acggcttaga
    15541 tgtctcgcga tcaatcagaa ccagcccgtc tgcggtagaa atcagatctt tcaagcgaac
    15601 cggatggaaa aatcccttgg cgtgcagccg accgaccagc cgtccgaacg ctttcatcag
    15661 ctgctgccgt tcggttccat tcgaccggtc aaagagtttt gcgagttcct cgcccttgac
    15721 ttcccggaca accagaaaac cgcgcaacgg aactcccagt aaacgctgct cgccccacgc
    15781 caccggctcc attgccagga atccgtttga gcgaagcatc tgaagcagcc gcacttcacg
    15841 caacggcccg gagtgcggac gcagtccgcg cagaaacatg ggaacacact ttttccagac
    15901 gggctcacct tcctcccgct tcaggtagaa agcggaaccg tcactcagag caaaccgctg
    15961 tattttttca gttccgcggt gcatcaaggc ttcaaaggaa gccagtcccg attctgcaaa
    16021 ctgcgactcc agcgccggct ctatatagta cgaaacggtt ttcatcagca gccccgctca
    16081 ccgacggtca ttcagaatct tcacaaacgc cagcgtcctc tcaggcttgc gttgattttc
    16141 tttcgtcgtc agctgcgaaa attcaaaaag ccgggccagc atcggaagat ccttctgagg
    16201 aattacgaga tagatattcc gtccgtcggc cagccgctca aagaccggct tcaaatcagc
    16261 aacgtcttta atcagaacaa ccggatggcg ggcgtaatac ggcaggatgt ccggaaactc
    16321 gctccacgcc gccagatagt caccgtcatt taccgcctgc ccgaccgaca
//
LOCUS       DAOPZO010000029        16215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_3815_length_16215_cov_9.027908, whole genome shotgun sequence.
ACCESSION   DAOPZO010000029 DAOPZO010000000
VERSION     DAOPZO010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 16215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16215
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3815_length_16215_cov_9.027908"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1463
                     /locus_tag="PKI68_03365"
     CDS             159..1463
                     /locus_tag="PKI68_03365"
                     /inference="COORDINATES: protein motif:HMM:NF017437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNX52764.1"
                     /translation="MMDFNKMTQKVREAFQSAQALAAEAGHQQIDGEHLLLALLRQQD
                     GLAPQLFERMEASAPTAMARLEQELKKLPSVSGPGTESGKIYVTQRLDGLLVKAHKEM
                     QKLGDEFLSVEHLLLAFADEKTKTAAGRVLDELGITRNRILDALKTVRGSQRVTSDNP
                     EGAYEALKKYGQDLVELARSGKLDPVIGRDAEIRSVIRILSRKTKNNPVLIGEPGVGK
                     TAIVEGLAHRIVRGDVPEGLKDKTIFALDMTSLMAGAKYRGEFEERLKAVLNEIKAAE
                     GRIILFIDELHNIVGAGRTEGSTDAGNMLKPMLARGELHCIGATTLDEHRKYIEKDAA
                     LERRFQPVLVDMPSVEDTISILRGLRERFEVHHGVRIKDTALVAAATLSDRYITERFL
                     PDKAIDLMDEACASIRTEIDSLPANWTKSAVKSCDWRSKKPP"
     gene            1433..2767
                     /locus_tag="PKI68_03370"
     CDS             1433..2767
                     /locus_tag="PKI68_03370"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX52765.1"
                     /translation="MRLEIEETALKKEKDKASKERLAALQKELADLKAEEKTFRTQWE
                     NEKGGIQKVRDLREALELARRESAEAERAYDLERVAKLRHGTIPALEKQLKELEQKLD
                     AQEGTKNLLREEVTEEEIAEVVARWTGIPLTKLLEGEREKLLKLADQLHRRVIGQDEA
                     VQTVADAVLRARAGISNPNRPIGAFLFLGPTGVGKTELARALAFELFDSDDHMVRIDM
                     SEYMEKHTVSRLVGAPPGYVGYEEGGQLTEAVRRKPYSVVLLDEIEKAHPDVFNILLQ
                     IFDDGRLTDSQGHTVSFKNTLIIMTSNIGSSLLLEGIGRQGRITEEIRDQVMAMLKEN
                     FRPEFLNRLDDTVLFKPLTEREIGQIVELLLNDLRKRLAARDIRLEVGDDVQKHIVEE
                     GFDPVYGARPLKRFIQQHFETQLARSLISGEITDGSSVQARLRDGRLVFEPA"
     gene            2842..>3737
                     /locus_tag="PKI68_03375"
     CDS             2842..>3737
                     /locus_tag="PKI68_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNX52766.1"
                     /translation="MKLKDRLKSGPLLLDGAAGTYLQGQPITREQWGAYEGCNEWLNL
                     SAPEVIRGLHRAYLAAGADGVETNTFGASPVTLGEYDLSHRAYEINRAAAALARECAD
                     EFSTPEQPRYVFGSIGPGTKLPSLSHIAFDPLVEAYKTQIQGLLDGGVDGLIIETCQD
                     PLQIKAALAAADDILGPNSEIVRYVSITVEATGTMLVGTSVPAAAAILAPYPIDVLGL
                     NCATGPDAMEHHLDTLGEIWPTRLACMPNAGMPEASPDGKVRYPLQPQPFAVKVASLA
                     KEHGLSIVGGCCGTTPEHIRELR"
     assembly_gap    3738..3837
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3838..6252
                     /locus_tag="PKI68_03380"
     CDS             <3838..6252
                     /locus_tag="PKI68_03380"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNX52767.1"
                     /translation="LFSPVELTQEPAPLYIGERANATGSKKFRDTLLANDYENAFHIL
                     TEQEETGAHVLDLSCGYAGRDESADIAVLVPRMARECRTPMMIDSTSADVIEQALKLY
                     GGRALINSINFEDGGKRAERVVPMARRYGAAMVALTIDEKGMAMTADEKVAIAKRLTE
                     FCVERGLKAEELLIDPLTFTVCSGDKTLYDAALQTLEAIRRIKAELPGVRTMLGLSNI
                     SFGLKPAARKVLNAVFLHQAVQAGMDACIIHVAAIVPLNEVPEALRKGAEALLNNDRS
                     NGDPLENYINLFETVQETAEVAVESRPSADVLADAIIRGKSPWLTPAIPDLLKVQSAE
                     DILNTILLPAMKEVGRLFNDGIMQLPFVLKSAEVMKRAVDMIKPFMKRSAAGDQVPLF
                     VIGTVAGDVHDIGKNLVGIILSNNGFDVVDLGIKVPVEQMMEAVKKHNAAALGMSGLL
                     VKSTAVMAENMKVLELAGFKTPVLLGGAALSEEFVDKACRPHYSGQVLYSKDAFAGLA
                     AMQTLKETGRLPDYVAPTRDGLGCEVKPAADEKPAEPINRDVPVPDVRIGTTLTEDIP
                     LDAIWNYLDLNGMIKGAWGYKQGKLSDAEYQKLLDDEVYPKLEEMKRIARGVFEPKVI
                     HGFFNCRADGDALILHNPAGGEIHMPFPRQKKAGGHCLADFFRPDQDICALMAVTLGN
                     KVMAKEHELRDADLYHDYLLFHGLAVMTAEALAETWHRKIRLLWGSDEGELTLAEIWK
                     RKLDQSRFGFGYALCPDLAMNKVCCDLLDTSRIGLNVTELFMADPECSTFALITHHPQ
                     AYYFDI"
     assembly_gap    6258..6357
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6596..6799
                     /locus_tag="PKI68_03385"
     CDS             6596..6799
                     /locus_tag="PKI68_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNX52768.1"
                     /translation="MSKGTVKWFNTEKGFGFITPDDGGKDLFVHHSEVKTPGRAALSE
                     GQKVDFETGEGQKGPCAKNVVPC"
     gene            6860..7138
                     /locus_tag="PKI68_03390"
     CDS             6860..7138
                     /locus_tag="PKI68_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNX52769.1"
                     /translation="MSPQRITVRYEGRVQGVGFRYTAVSLAQGLDVTGRVKNEFDGSV
                     SLVAEGAEEQLMELLQAIRRSHLGRYITSELVKRSPATGEFKGFGVQH"
     gene            complement(7113..7799)
                     /locus_tag="PKI68_03395"
     CDS             complement(7113..7799)
                     /locus_tag="PKI68_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNX52770.1"
                     /translation="MSAWAIVLACGKDQEIGAGVDVAFLALGSRPVVARSIQTLEQNP
                     LIEGIILVVRKERVDSAMHTVRSFGCRKISAIVAGGPARLTNLKNALERVPDDATAVL
                     IHEATRPFLNDEIITETVKAGKRYGAAVAAVKSADAVKLAEKGQKVTRSLDRNSVWLV
                     QSPQVYKIDVFKKMIKGSGKLIDDDSELLAKGKQEIHLVAGSPGNMKIRTPDDLQIAS
                     AILSVARRTP"
     gene            complement(7943..8863)
                     /locus_tag="PKI68_03400"
     CDS             complement(7943..8863)
                     /locus_tag="PKI68_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNX52771.1"
                     /translation="MKKSIAPKLVIVGSIGIDTIETPREKRIEILGGSVSYACAAASF
                     FVKTGMVGVVGTDFPPEHRALWEKMGIDLAGLQTEAGKTFRWSGVYEENMDNRRTLLT
                     ELNVFESFSPELPDTYRGAPYLFLGNIAPSLQLHVLEQVHSPKFVLIDTMDLWINIAR
                     EQLVRVVSKCDMLTLNESEARLFTGAHSLMEAARALLAMGPRFVLIKKGQSGSMLFSK
                     TGIFMLHAFPLADFKDPTGAGDTFAGGLMGALAESDGTDEKSIRRAMVYGSVTAAFGV
                     EEFSLERLAALDRKQIDARADQLKSMCRID"
     gene            complement(8860..9408)
                     /gene="amaP"
                     /locus_tag="PKI68_03405"
     CDS             complement(8860..9408)
                     /gene="amaP"
                     /locus_tag="PKI68_03405"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HNX52772.1"
                     /translation="MTKFLHVMSGLMIWLLFFALGGALIHANIPGVTQSAFDLIPPLQ
                     SWEMAGIGGLIILLSMLYLVTFGPRRQKKRYITYDSGSGSVSISDNAVREYIRKLSGE
                     FGMVVSIEPKIRTERDAISIDLDVKLVAGSRIPEISQALQERVRESLREGLGIADVKE
                     VKVRIQEIVGEPSASSRRGWRS"
     gene            complement(9410..9829)
                     /locus_tag="PKI68_03410"
     CDS             complement(9410..9829)
                     /locus_tag="PKI68_03410"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNX52773.1"
                     /translation="MDRNVPPSRNWPVPEEKSDDAGLGAVEIHNNVIAVIAHEAAGRV
                     PGVVELSGTFVDELADIIGKRPRDRGVRVAVDSENTIIVKLTAVLEYGVYIPEVCGKL
                     QLEVRKSIEEMTGKRVQAVNISVQSIRRSVKDVAGGL"
     gene            complement(9842..11209)
                     /gene="accC"
                     /locus_tag="PKI68_03415"
     CDS             complement(9842..11209)
                     /gene="accC"
                     /locus_tag="PKI68_03415"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNX52774.1"
                     /translation="MFEKILIANRGEIALRIIRACKELGIKAVAVYSEADAESLHVQM
                     ADEAICIGPASATQSYLKMANIISAAEVADVDAIHPGYGFLAENAHFAEICRSCNITF
                     IGPTPEVIRQMGDKAIARDTMKAAGVPITPGSDGVLSTKEEALELAHKMGYPVLIKAV
                     AGGGGKGMRVAHNDVSLAQGFMMASAEAGSAFGNPDVFMEKYIESARHVEVQVIGDNH
                     GNVVHLGERDCSIQRRHQKLVEESPCPVLTPDERKALGEAGVKAAKAVGYNSAGTLEF
                     LYDEKAKKFYFMEMNTRIQVEHTVTEEVTGIDLVKEQILVAAGKKLSFTQDDVKARYH
                     AIEFRVNAEDPYKNFTPSPGKVDAVHFPGGPGIRIDSHVYSGYTISPYYDSMIAKIIA
                     RGATRDEALNRLHRALEEFTINGPHTSVPVGIALLMDSRFRRGEYNTAFLEKFMNENF
                     MVDRQ"
     gene            complement(11324..11806)
                     /gene="accB"
                     /locus_tag="PKI68_03420"
     CDS             complement(11324..11806)
                     /gene="accB"
                     /locus_tag="PKI68_03420"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNX52775.1"
                     /translation="MELKEIKKIVEMMTENDLAEFLLEEEAFTLQLKRGTAGITQIVS
                     APQQMMAAPAVAAPVAGVPAAVAPVAAAPVAANDDAGLVAIKSPMVGTFYRSPSPDSD
                     VFVQIGQDVTPDSVVCIIEAMKVMNEIQAEVKGKIKKILVDNATPVQFGQPLFLVEPA
                     "
     gene            complement(11851..12357)
                     /gene="ruvC"
                     /locus_tag="PKI68_03425"
     CDS             complement(11851..12357)
                     /gene="ruvC"
                     /locus_tag="PKI68_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNX52776.1"
                     /translation="MNKVRILGVDTSLRSSGVAVIEVSGQQMRALAYGRIHNKPALSH
                     SGCLAAIHREIDKLITEFSPDEAAIEGAFFAKNAKTAMILGQARGAVLAACALRSLPV
                     YEYSPRSIKQATVGAGAASKEQVGKMVMRLLGLAELPQEDAADALAAAICHFHQRALP
                     EALRSKPV"
     gene            complement(12354..13103)
                     /locus_tag="PKI68_03430"
     CDS             complement(12354..13103)
                     /locus_tag="PKI68_03430"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNX52777.1"
                     /translation="MSGHSKWANIKIKKGKADAARGKVFSKIAKEMTVSARMGGGDPA
                     GNITLRALIAKAKSVNMPNDNIERAIKKGTGELASAALEECVYEAYAPGGVGLVIQAL
                     TDNKNRAASEIRNVCTKNGASMAQQGAVSRSFQRKGQIYIDAAGIDEDTLMNIVLEAG
                     AEDMQRDGDQFEVLTAFTDYAAVCEALSKAGIATASSELTMLPEMPVEIKDKAKAQQV
                     MKIVEALDELDDVQNVYGAFEIPDAILAEME"
     gene            complement(13222..13875)
                     /locus_tag="PKI68_03435"
     CDS             complement(13222..13875)
                     /locus_tag="PKI68_03435"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNX52778.1"
                     /translation="MKKSEAAWAEERREMVRLLRERYHIRDERVLRAMAEIPRHLFIP
                     ETFRPFADPYGDHPGPIGHGQTISQPYIVAYMTEKLNPQEGDKVLEIGAGSGYQSAVL
                     AALGAEVYTIETVPDLAEHARQVLAEQGFSKVTVRCGDGYAGWPEAAPFDVIIGTCSP
                     EDVPSALVDQLAEGGRMMLPVGLGRQRLVILRRCGYGLMQEDDLPVRFVPMIHPPEA"
     gene            complement(14031..15470)
                     /locus_tag="PKI68_03440"
     CDS             complement(14031..15470)
                     /locus_tag="PKI68_03440"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX52779.1"
                     /translation="MFQDKSFPFQITDVVGLSPFLRLRKETTKVVFAHLPHLALHFGV
                     MSSRDAALLSLFDGRRNLEDVIIKASILTDTSYLQARKTMYDLMKAVSPDFQVFVNCA
                     YSSHKVFDASDYMIAAEKIDINVRLEAPLTMMLQPTPLCEVDCVYCYACRRPLKAREC
                     LSLARIVELLDEAVALGIYQINLCGGDGFCREDFVEIVAACRQRDLIVDLSTKCRIDK
                     NMARRLADAGLDYIQVSLDSYKEETADLMYGRKGHFRNIVTSIENLVESGIFTRTNSI
                     VTTYNFNDIRQTIGFIKSLGVRELKFSPAFRSMYRDNEHILLSFEQKEEYRRLMRELE
                     PQTRSEGLVVFHDAMKDFSEMTPAEKREYWFEKRARCSAGRCSLVITPDGNVVPCEEM
                     PQTEEYFVGDVRTQSIKEVWNSPKLLQFTYPSRESFAGSTACYACADFDWCTHAMGHC
                     FRDSLKVYGDVYETNPFCPRAKATTNRIY"
     gene            complement(15457..15882)
                     /locus_tag="PKI68_03445"
     CDS             complement(15457..15882)
                     /locus_tag="PKI68_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52780.1"
                     /translation="MKKATQGRDIVAIEKLLNDKDPQTAGKVKEFLQSTKGAELQGLL
                     GLKSLLEEQGYSRQDAVAEGKSYCENIVCNLAACVFSSSCSNGACSSAGCSANSGGII
                     TPPAECKGANTCGTQACSSNANSGGNGTCGNATCSYVSG"
BASE COUNT         3600 a         4699 c         4425 g         3291 t
ORIGIN      
        1 tttttcatcg gctgcgccag cttggcgcac aaggtccgcg cgctgaattt tataaaaatt
       61 tcataaccga ttcgcaataa gcaggttaat atccatctgc acccgctggc acatccattg
      121 ctttttcatc tggcgacttt agacgaaagg agagtctcat gatggacttc aacaaaatga
      181 cccagaaggt acgcgaggcg tttcagtcgg ctcaggcttt agcggcggag gccggtcacc
      241 agcagatcga cggcgagcac ctgttgcttg cgctgcttcg tcagcaggac ggactggcgc
      301 cgcaattatt tgagcggatg gaagcttccg ccccgaccgc gatggcgcgg ctcgaacagg
      361 aacttaaaaa actgccgtcg gtttccggcc ccggcaccga aagcggcaag atttatgtca
      421 cccagcggct cgacggtctg ctggtcaagg ctcacaagga gatgcagaag ctgggcgacg
      481 agtttctctc ggtcgagcat ctgctgctgg cctttgccga cgaaaaaaca aaaaccgccg
      541 ccggacgggt tctcgacgaa ctcggcatta cccgcaaccg gattctcgac gcgctcaaaa
      601 ccgtgcgcgg cagtcagcgc gtcacgtccg acaatccgga aggggcgtat gaagcgctga
      661 agaaatacgg gcaggatctt gtggagctcg cccgctccgg caagctcgat ccggtcatcg
      721 gccgcgatgc ggaaatccgt tcggtgatcc gcattctgtc gcgcaaaacc aaaaacaatc
      781 cggtgctaat cggcgaaccc ggcgtcggca aaacggcgat tgtcgaaggg ctggcgcatc
      841 gcatcgtgcg cggcgacgtt ccggaagggc tgaaggacaa aaccattttc gcgctggata
      901 tgacgtcgct gatggccggc gccaaatacc gcggcgaatt cgaggagcgc ctcaaagcgg
      961 tgctgaatga aatcaaggcg gcggagggcc gcattattct gttcatcgac gagctgcata
     1021 acattgtcgg cgcgggccgc accgaaggtt cgaccgacgc gggcaatatg ctcaagccga
     1081 tgctggcgcg cggcgaactg cactgtatcg gggcgacgac gctcgacgag caccgcaagt
     1141 acatcgaaaa agacgccgcg ctcgagcgcc gcttccagcc ggtcctcgtc gatatgccca
     1201 gcgttgaaga caccatttcg attctgcgcg gactgcgcga gcggttcgaa gtgcaccacg
     1261 gcgtgcggat taaagatacc gcgctggtcg ccgcggccac gctctccgac cgctacatca
     1321 cggaacggtt cctgccggac aaagccatcg acctgatgga cgaagcctgc gcctccatcc
     1381 gcaccgagat cgattcgctg cccgcgaact ggacgaaatc agccgtaaag tcatgcgact
     1441 ggagatcgaa gaaaccgccc tgaagaagga aaaagacaag gccagcaagg agcggctcgc
     1501 cgcgctgcaa aaagagctgg ccgatctgaa ggcggaagaa aagaccttcc gcacgcagtg
     1561 ggaaaacgaa aaaggcggca tccagaaagt ccgcgacctg cgcgaagcgc tggagctcgc
     1621 gcggcgcgaa agcgccgagg cggaacgcgc ctacgacctg gagcgcgtcg ccaaattgcg
     1681 ccacggaacc attcccgcgc tcgaaaaaca gctgaaggag ctcgaacaga aactcgacgc
     1741 gcaggaaggg acaaaaaatc tgctccgcga agaggtgacg gaagaggaaa tcgccgaagt
     1801 cgtcgcccgc tggaccggca tcccgctcac caaactgctt gaaggcgaac gcgaaaaact
     1861 gcttaagctc gccgaccagc tccaccgccg cgtgatcgga caggacgaag ccgtccagac
     1921 ggtcgccgat gccgtcctgc gcgcccgcgc cggcatcagc aatccgaacc gcccgatcgg
     1981 cgcgttcctg tttctgggcc cgaccggcgt cggcaaaacc gaactggccc gcgcgctcgc
     2041 cttcgaactg ttcgacagcg acgaccatat ggtgcgcatc gacatgagcg agtacatgga
     2101 aaaacatacc gtctcgcggc tggtcggcgc gccgcccggc tatgtcggct acgaagaagg
     2161 cggacagctc accgaagcgg tgcggcgcaa gccctactcc gtcgtcctgc tcgacgaaat
     2221 cgaaaaagcg catccggacg ttttcaacat cctgttgcag attttcgacg acggacggct
     2281 gaccgactcg caaggccaca ccgtcagctt caaaaacacc ctcatcatca tgaccagcaa
     2341 catcggctct tcgctgctgc tcgaaggtat tggacgacag ggccgcatta cggaagaaat
     2401 ccgcgatcag gtcatggcga tgcttaaaga aaacttccgt ccggaattcc tgaaccggct
     2461 cgacgacacc gtcctgttca agccgctcac ggaacgggaa atcggacaga ttgtcgaact
     2521 gctactgaac gacctgcgca aacggctcgc cgcccgcgac atccggctcg aagtcggcga
     2581 tgacgtccaa aagcacatcg tcgaagaagg cttcgatccg gtctacggcg cgcgtccgct
     2641 caaacggttc atccagcagc attttgaaac gcagctggcc cgcagcctga tctccggcga
     2701 aatcaccgac ggctcgtccg tgcaggcccg gctccgggac ggccgactgg tttttgagcc
     2761 ggcctgacgg taaaaatccg tttatccgga ttttaggatt aatggttgcg aatcccgaga
     2821 cggtctgtta gctttcgcga catgaaactc aaagaccgat tgaaatccgg acccctgctg
     2881 ctcgacggcg cggccggcac ctatttgcaa ggccagccca tcacccgcga gcagtggggc
     2941 gcatacgaag gctgcaatga gtggctgaat ctttcggccc cggaggtgat ccgcgggctg
     3001 caccgcgcct atctcgccgc cggcgcggat ggggtggaaa ccaatacctt cggcgcgtcg
     3061 ccggttacgc tcggcgaata cgatctgtcg caccgcgcct acgaaatcaa ccgggccgcc
     3121 gccgccctcg cccgcgagtg cgccgatgaa ttttcgacgc cggagcagcc gcgctatgtc
     3181 ttcggctcca tcggccccgg cacgaaactg ccgtcgctga gccacatcgc gttcgatccg
     3241 ctggtcgagg cctacaagac tcaaattcag ggtctgctcg acggcggcgt cgacgggctg
     3301 attattgaga cctgtcagga cccgctccag atcaaggcgg cgctggcggc agccgacgat
     3361 attctcggcc cgaacagcga gattgtccgc tatgtttcca tcacggttga agccaccggc
     3421 acgatgctgg tcggaaccag tgttccggct gccgccgcga ttctggcgcc gtacccgatt
     3481 gatgtgctcg gcctcaactg cgccaccggc cccgacgcga tggagcatca tctcgatacg
     3541 ctcggcgaga tctggccgac ccgcctggcc tgtatgccga acgccgggat gccggaagct
     3601 tcgccggacg gcaaggtccg ctatccgctg cagccgcagc cgttcgccgt aaaagtcgcc
     3661 tcgctggcca aggagcacgg gctgtccatc gtcggcggat gctgcggcac cacgccggag
     3721 catattcgcg aactgcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctg
     3841 ttctcgccgg tcgagctgac gcaggagcct gcgccgctgt atatcggcga gcgcgccaac
     3901 gcgaccggtt cgaaaaagtt ccgcgacacg ctgctggcga atgattacga gaacgccttt
     3961 cacattctga cggagcagga agagaccggc gcgcatgtgc tggatttgag ctgcggttac
     4021 gccgggcgcg acgagagcgc agacattgcc gtactggttc cgcgcatggc gcgcgagtgc
     4081 cgcacgccga tgatgattga ttcgacctcg gccgatgtga ttgagcaggc gctcaagctc
     4141 tacggcgggc gggcgctgat caactcgatc aatttcgaag acggcgggaa gcgcgcggaa
     4201 cgggtggtgc cgatggcacg gcgctacggc gcggctatgg tcgcgctgac gattgatgaa
     4261 aaaggcatgg cgatgacggc ggatgaaaag gtcgcgatcg ccaaacggct gacagagttc
     4321 tgcgtggagc gcggactgaa ggccgaagaa ctgctgatcg atccgctgac gtttacggtg
     4381 tgcagcgggg ataagacgct gtatgacgcc gcgcttcaga cgctggaagc gatccggcgc
     4441 atcaaggcgg agctgccggg cgtgcgcact atgctggggc tttccaacat ttcgttcggg
     4501 ctcaaaccgg cggcgcgcaa agtgctcaac gccgtgttcc tgcatcaggc ggtacaggcc
     4561 ggcatggacg cctgcatcat tcacgtcgcg gcgattgtcc cgctgaatga agttccggaa
     4621 gcgctgcgca aaggcgccga ggcgctgctg aacaatgacc gctcgaacgg cgacccgctg
     4681 gaaaactaca tcaacctgtt tgagaccgtc caggagacgg ccgaggtcgc ggtggaaagc
     4741 cggccgtcgg ccgatgtgct ggcggatgcg atcattcgcg gcaagtcgcc gtggctcacg
     4801 ccggccattc ccgatctgct gaaagtgcag agcgccgagg atattctcaa cacgatcctg
     4861 ctgccggcga tgaaagaggt cgggcggctg ttcaacgacg gcatcatgca gcttccgttt
     4921 gtactgaagt cggcggaggt gatgaagcgc gcggtggata tgatcaaacc gtttatgaag
     4981 aggagcgcgg cgggcgatca ggttccgctg ttcgtcatcg gcaccgtggc gggcgacgtg
     5041 cacgacatcg gcaaaaacct cgtcggcatt attctttcca acaacgggtt cgatgtcgtc
     5101 gacctcggca tcaaggtgcc ggtcgagcag atgatggagg cggtgaaaaa gcataacgcc
     5161 gcagcgctcg gcatgagcgg cctgctggtg aaatccacgg cggtgatggc ggaaaacatg
     5221 aaagtgctcg agctcgccgg atttaaaacc ccggtgctgc tcggcggcgc cgcgctctca
     5281 gaagagtttg tcgacaaggc ctgccggccg cactattccg gccaggttct ctacagcaaa
     5341 gatgccttcg ccgggctggc cgccatgcag acactcaagg aaaccggccg gctgccggat
     5401 tatgtggcgc cgacccgcga cggcctgggc tgcgaagtca aaccggcggc cgatgaaaaa
     5461 ccggccgaac cgattaaccg cgacgttccg gttccggacg tccggatcgg caccaccctg
     5521 acggaagaca tcccgctcga cgcgatctgg aactatctcg atctgaacgg aatgattaag
     5581 ggcgcgtggg gctacaagca gggcaagctc tccgacgccg aataccagaa actgctcgac
     5641 gacgaagtct atccgaagct cgaagagatg aagcggatcg cgcgcggcgt gtttgaaccg
     5701 aaagtgattc atggattttt caactgccgc gccgacggcg acgcgctgat tctccataat
     5761 ccggccggcg gcgaaatcca catgcccttc ccgcgccaga aaaaagcggg cgggcactgc
     5821 ctcgccgact tcttccggcc ggatcaggac atctgcgcgc tgatggccgt gacgctcggc
     5881 aataaggtga tggcgaaaga gcacgaactg cgcgacgccg acctttacca cgactatctg
     5941 ctgttccacg gactggccgt gatgaccgcc gaggcgctgg ccgaaacctg gcaccggaag
     6001 atccgtctgc tctggggttc ggacgaaggc gagctgacgc tggccgaaat ctggaaacgc
     6061 aaactcgacc agagccgttt cggcttcggc tacgcgctct gccccgacct ggcgatgaac
     6121 aaggtctgct gcgacctgct ggacaccagc cggatcggcc tgaacgtcac cgagctcttc
     6181 atggccgacc cggaatgctc gacctttgcg ctcatcacgc atcatccgca ggcgtattat
     6241 tttgatattt gatggtannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatg
     6361 gccaaaccca attacacgtt cgagaagcgc cagaaagagc tcgccaaaaa gcggaagaag
     6421 gaagaaaaac ggcagcggaa agcaggcgca tcgaacgaac ttccggtgac tgattcgccg
     6481 caggcgcccg gcgcctgagt tcaaattgga aagctgtttt caaatcagtt tacggcagga
     6541 aaacgttgag tcagattgcg gcagtaaaat taacatggat aaggagacgt gcgttatgag
     6601 taaaggtaca gtgaagtggt tcaatacaga aaaaggtttc ggatttatca cccctgatga
     6661 cggcggaaaa gatctgttcg tgcatcattc cgaagtcaaa acaccgggac gcgccgctct
     6721 cagtgaaggt cagaaagtgg acttcgaaac cggagaaggc cagaaaggcc cgtgcgctaa
     6781 aaacgttgtg ccctgctgac ggttcagccg gataaagatg caggtgccgg caaaaaaccg
     6841 cgcctgttct gttttgatta tgagcccgca acgaataact gtgcgttatg aaggccgcgt
     6901 gcagggcgtt ggattccggt acacggcggt gagtctggcg cagggactgg atgtaaccgg
     6961 acgggtgaag aatgagttcg acggaagtgt gtcgctggta gcagaaggcg cagaggagca
     7021 actgatggaa ttactgcagg cgatccgccg ttcgcacctc ggaagatata tcaccagcga
     7081 gctggtgaaa cgctcgccgg caaccggtga gtttaagggg ttcggcgtgc aacactgagg
     7141 atggccgagg cgatctgaag gtcgtccgga gtccggattt tcatgttgcc cggcgaaccg
     7201 gcaaccagat gaatctcctg ttttcctttc gccagcagtt cagagtcgtc gtcgatcagt
     7261 ttgccggagc ctttgatcat ttttttgaag acgtcgattt tataaacctg cggactctgc
     7321 accagccaga cggaattgcg gtcgagcgat ctggtgactt tctgtccttt ttcagccagc
     7381 ttgacggcat cggcactttt cacggcagcg acggcggcgc cgtagcgttt tccggcttta
     7441 acggtttccg tgataatttc gtcgttcagg aacgggcggg tcgcctcatg aatcagcacg
     7501 gcagttgcat cgtccggaac ccgttccagc gcatttttaa gattggttaa acgcgccgga
     7561 ccgccggcga cgatggcgga aattttgcgg catccgaagg atcggactgt atgcatcgca
     7621 ctgtcaacgc gttctttacg aaccacgagg ataatgcctt caatgagcgg attctgctcc
     7681 agtgtctgaa tggatcttgc aacaaccggc cgggagccga gcgcaagaaa tgcgacatcg
     7741 acacctgctc cgatttcctg atctttaccg cacgctagaa caatcgccca tgctgacatg
     7801 ttttacgctc ctttcggcgt cgtacaaatg caactcgcga gatggcagga atttaacaag
     7861 actgcaacgg gctgtcaaac aatgcggtga ttccgaacca taaaaaatga ccccgggaat
     7921 cgccgatgcg cagatgctgg cattagtcta tgcggcacat agacttaagc tggtcggcac
     7981 gggcgtctat ttgtttccgg tcaagagcag caagacgctc cagactgaac tcttcgacgc
     8041 caaaagcagc cgtcacgctg ccgtaaacca tagcgcggcg aatcgatttt tcatctgttc
     8101 catccgattc ggccagcgcg cccatcaggc cgccggcaaa agtgtcgccc gcgccggtcg
     8161 gatctttgaa gtccgccagc ggaaaagcat ggagcatgaa aattccggtt tttgaaaaga
     8221 gcatacttcc gctctgccct tttttgatga gtacaaaacg cgggcccatg gcgagaagcg
     8281 ccctggcggc ttccatcagc gaatgcgcgc cggtaaagag gcgcgcttcg gattcgttga
     8341 gggtgagcat atcgcactta ctgacaaccc gaacaagctg ttcgcgggcg atgttgatcc
     8401 agagatccat cgtatcgatc agcacaaact tcggcgaatg cacctgttcg agaacgtgaa
     8461 gctgcagtga cggagcgatg ttgccgagga aaagataagg cgcgccgcga taggtgtccg
     8521 gcagttccgg cgaaaagctt tcgaaaacgt taagttcggt gaggagtgtg cggcggttgt
     8581 ccatgttttc ttcatagaca cctgaccagc ggaatgtttt gcccgcttcg gtctgaagac
     8641 cggcgaggtc gatgcccatc ttctcccaga gcgcacgatg ttccggaggg aagtccgttc
     8701 cgacgacgcc gaccatgccg gttttaacaa agaaagaggc tgcagcacag gcatagctga
     8761 cagaaccgcc cagaatttcg atgcgctttt cgcgcggagt ttcaatggtg tcgataccga
     8821 tagagccgac gatgaccagt ttaggggcaa tagatttctt catgatctcc aaccacggcg
     8881 ggacgatgcg gacggctcac cgacaatttc ctgaatacgc actttgactt ccttcacgtc
     8941 ggcgatgccg agcccttcgc gcagactttc gcggacacgt tcctgcagcg cctgggaaat
     9001 ttctggaatg cgggaaccgg ccaccagctt tacatccaga tcaatgctga tcgcgtcacg
     9061 ttcagtacgg attttcggct caatgctgac aaccatgccg aattcgccgc tgagtttgcg
     9121 gatatactcg cgcacggcat tatcgctgat cgaaacggac ccgctgccgg aatcataggt
     9181 aatgtagcgc ttcttctggc ggcgcggacc gaacgtgaca aggtaaagca tcgaaagaag
     9241 aatgatgagt ccgccgatac ccgccatttc ccagctttga agcggcggaa tcagatcgaa
     9301 agcgctttgc gttacaccgg gaatgttggc atggatcagt gcaccgccca gcgcaaaaaa
     9361 cagcagccag atcatcagtc cggacattac atgaagaaac ttggtcattt tacagccctc
     9421 ccgcaacatc ctttacactg cgccgaatgc tctgcaccga aatattaacc gcctgcaccc
     9481 gcttgccggt catttcttcg atcgacttac ggacttcgag ctgaagtttt ccgcagactt
     9541 cgggaatata aacgccgtat tcgagcacgg cggtcaactt aacgatgatc gtgttttccg
     9601 aatcaacggc aacgcgcacc ccgcggtcgc ggggacgctt gccgataata tcggccagtt
     9661 cgtcaacaaa cgttccggag agttcaacca cgccgggaac gcgaccggcc gcttcatgag
     9721 cgatgaccgc gatgacgtta ttgtgaatct cgaccgcacc aagtccggca tcgtccgact
     9781 tttcctcagg aacgggccag tttctggaag gcggaacgtt tctgtccata gccgtatctc
     9841 cttactgccg gtctaccatg aaattctcat tcatgaattt ttcaaggaag gccgtgttgt
     9901 attcgccgcg gcggaagcgg ctgtccatca gcagagcaat accgaccggt acgctggtat
     9961 gcgggccgtt aatggtgaac tcttcgagcg cgcggtgcag gcggttgagc gcctcgtcgc
    10021 gcgtggctcc gcgggcgatg atcttcgcga tcatactgtc gtaatacggc gaaattgtgt
    10081 agccgctgta aacgtgcgaa tcgatccgga tgccggggcc gccggggaaa tgcaccgcgt
    10141 ccactttgcc gggcgacgga gtgaaatttt tgtaaggatc ttcggcgttc acgcggaatt
    10201 caatggcgtg atagcgtgct ttcacatcgt cctgagtaaa ggaaagcttt ttgccggccg
    10261 caaccaggat ctgttctttc accaggtcga tacccgtcac ttcttccgtg accgtatgct
    10321 caacctgaat gcgggtgttc atttccatga aataaaactt cttggccttt tcgtcgtaaa
    10381 ggaactcgag cgttccggcg ctgttatagc ctaccgcctt ggcggctttg acgccggctt
    10441 cgccgagcgc tttgcgctcg tcgggcgtaa gcaccggaca gggcgactct tccaccagtt
    10501 tctgatggcg gcgctgaata ctgcaatcgc gctcgccgag atgcaccacg ttgccgtggt
    10561 tgtcgccgat gacctgcact tcaacgtggc gcgcagactc gatatatttt tccatgaaca
    10621 catcgggatt tccgaaggcg ctgccggctt cggcgctggc catcataaag ccctgagcca
    10681 gactgacgtc gttatgcgcc acgcgcatcc cttttccgcc gccgcctgca accgctttaa
    10741 tgagaaccgg atagcccatt ttgtgagcca gttctaaagc ctcttccttc gtcgaaagaa
    10801 caccgtcgct gccgggcgtg atcggcacgc cggctgcctt catcgtatcg cgcgcaatcg
    10861 ctttatcgcc catctggcgg atgacttccg gcgtcgggcc gatgaaagtg atgttgcagc
    10921 tccggcagat ttcggcgaag tgcgcgttct cggccagaaa accgtagccg ggatgaatgg
    10981 cgtccacgtc agccacttcg gcggcgctga taatgttggc cattttgaga tagctctggg
    11041 tggcggaagc cggtccgata caaatggctt catcggccat ctggacgtgg agcgattccg
    11101 catcggcctc tgaataaacc gcaaccgctt tgattcccag ctctttacag gcccggataa
    11161 tccgcagcgc gatctcgcca cgattggcaa tcagtatttt ttcaaacata gaaacccttt
    11221 gggttaaatc cgaaatctaa aacccgaagc acgaaacaat ttctaaattc aaaaactcca
    11281 aacccgtttc gaatttcaag attcgtgctt cgaattttca aagttaagcc ggctcgacga
    11341 ggaagagagg ctgaccgaac tgaacgggcg tggcgttatc caccagaatt ttcttaatct
    11401 tacccttcac ttcagcctga atctcgttca tcaccttcat ggcctcgata atgcagacca
    11461 cggaatcagg cgtaacatcc tgcccgatct gaacaaagac atcggaatcc ggagacggcg
    11521 aacggtagaa ggttccgacc atcggggact taatggcaac cagcccggcg tcatcgttcg
    11581 cagcaaccgg agcggcagcg accggggcga cggcagccgg tacgccggcg acaggagcag
    11641 caaccgccgg agcggccatc atctgctgcg gggcggaaac aatctgggtt atgcccgctg
    11701 tgccgcgttt gagctgcagt gtaaaggctt cttcttcgag cagaaattca gcgagatcat
    11761 tctcggtcat catctcaacg attttcttga tttccttgag ttccatcagg tgtttctcct
    11821 ttttttctta aacagggcac ggaaactaca ttaaaccggt tttgaacgca aggcttccgg
    11881 cagggctcgt tggtgaaaat ggcagatcgc cgccgcaagt gcatccgcgg catcctcttg
    11941 cggaagttcg gccagcccga gcaggcgcat aaccatcttc ccgacctgct ccttgctggc
    12001 ggctcccgcc ccgacggtcg cctgcttgat cgagcgcggc gagtattcat agaccggcag
    12061 actgcgcagc gcacaggcgg ccagcaccgc gccgcgcgcc tgacctaaaa tcatcgccgt
    12121 tttggcgttt ttggcaaaaa aagcgccctc aatggcggct tcatcggggg aaaactccgt
    12181 gatcagctta tcgatttcgc ggtgaatcgc cgccagacag cccgaatgtg acagcgcagg
    12241 tttattatga atccgcccat aggccagcgc gcgcatttgc tggccggaga cctcaatcac
    12301 cgccacgccg gacgagcgca gcgaggtatc gacccctaaa atgcggactt tattcattcc
    12361 atttccgcca gaatggcgtc gggaatttca aatgcaccgt acacgttctg gacgtcatcg
    12421 agctcatcca gcgcttcaac aatcttcatc acctgctggg ccttggcttt atccttaatc
    12481 tccaccggca tttcaggaag catagtgagt tccgaggagg ccgtggcaat acccgccttc
    12541 gacagcgctt cgcaaacggc ggcataatcg gtaaacgccg tcagcacttc aaactggtcg
    12601 ccgtcgcgct gcatatcttc cgcgcccgcc tcgagcacga tattcatcag cgtatcttca
    12661 tcaatcccgg cggcatcgat ataaatctgt cccttacgct ggaaactgcg gctgaccgcg
    12721 ccctgctggg ccatgcttgc gccgttcttc gtgcagacat tgcggatttc ggaggccgca
    12781 cggttcttat tgtcagtcag cgcctgaata acaagtccga ctccgccggg cgcatacgct
    12841 tcgtacacgc actcttcgag cgcggcgctg gccagctcgc cggtaccttt tttgatggcg
    12901 cgttcgatgt tgtcgttggg catattcaca gacttggcct tggcgatcag agcgcgcaag
    12961 gtaatgttgc ccgccgggtc gccgccgccc atacgcgccg atacggtcat ttccttggca
    13021 atcttactga aaactttgcc gcgcgcggca tccgctttgc cctttttgat cttaatattc
    13081 gcccacttac tatgacccga catgaaaccc tccgtctgct tcgcaaagtt atacgttatc
    13141 tgttaaaagt tatccgcctt cagcttccag cctgcgaacc ggtaactgat aaccgataac
    13201 catccggaaa taaaccgttt tttacgcttc cggcggatga atcatcggca caaaacgcac
    13261 cggaagatcg tcttcctgca tcagtccgta tccgcaccgc cgcagaatca caagacgctg
    13321 gcgcccgagg ccgaccggca gcatcatccg cccgccctcg gccagctgat caaccagcgc
    13381 cgacggcaca tcttccggcg aacaggtacc gataatcaca tcaaacggcg cggcctccgg
    13441 ccagccggca tagccgtcgc cgcagcgcac cgtaacctta gagaaccctt gttccgccag
    13501 aacctgccgc gcgtgttccg ccagatcggg aactgtttcg atggtataaa cttccgcgcc
    13561 cagcgccgcc agcaccgccg actgatagcc ggagcccgcg ccgatctcca gaaccttatc
    13621 gccctcctgc ggattcagct tctccgtcat gtacgccacg atatacggct gggaaatcgt
    13681 ctggccgtgc ccgatcggac ccggatgatc gccgtacgga tcggcaaacg gacgaaacgt
    13741 ctccggaata aaaagatggc ggggaatctc cgccatcgcg cgaagtaccc gttcgtcgcg
    13801 gatatgataa cgctcccgca gaagcctcac catctcccgg cgttcctccg cccaggctgc
    13861 ttcactcttc ttcacagacc tccgttcgaa agtttctaat gctcggaacc cagccttcgc
    13921 cgaagggtct ggaggagctg gagttcacat attttccgat tgcttggaag aaaagtttcc
    13981 acacattgga aattactaga ttgcgcaatc gatcccgttt ctaccttttc tcaatagatt
    14041 ctgtttgtcg tcgctttggc gcgcggacag aacggatttg tttcatacac atcgccatag
    14101 accttcaaac tgtcccggaa acaatgcccc atggcatgtg tacaccagtc aaaatcagca
    14161 cacgcgtagc aggctgttga accggcgaaa ctttcgcgcg acggataagt aaactgcaat
    14221 aacttcggcg aattccacac ttctttgatg ctctgcgtcc tgacatcccc cacgaaatat
    14281 tcttccgttt gcggcatctc ttcgcacgga acaacgttgc cgtcaggcgt aatgaccaga
    14341 ctgcaccggc cggcagaaca gcgcgccctt ttctcaaacc agtactcccg cttctctgcg
    14401 ggtgtcatct ctgagaaatc cttcatggca tcatgaaaaa caaccagccc ctcactcctt
    14461 gtttgcggtt ccagctcccg cataagcctg cggtattctt ctttctgctc aaacgaaagc
    14521 agtatgtgtt cattgtcccg gtacattgaa cggaatgccg gtgaaaactt caactcccgc
    14581 acaccaaggc ttttaataaa gcctatagtt tgtcggatat cgttaaaatt atacgtcgta
    14641 acgatgctgt tggttctggt gaaaattccg ctctcaacca gattttcgat gcttgtgacg
    14701 atgttacgaa aatgcccttt ccggccatac atcaaatcag cggtctcttc cttataggaa
    14761 tccagactca cctggatata atccaatccg gcatcggcaa ggcgacgtgc catattttta
    14821 tcgatacggc atttggtgga caaatcgacg atcaaatccc gttgtctgca ggcagcgaca
    14881 atttcgacaa aatcttcccg gcaaaaccca tccccgccgc aaagattgat ctgataaatt
    14941 ccaagcgcaa ccgcctcatc cagcaattca actattctgg ccagcgacaa acattcgcgt
    15001 gcctttaatg gacggcggca agcgtaacaa tatacacaat ctacctcaca caacggagta
    15061 ggctgaagca tcatggtaag cggcgcctcc agccgcacat tgatatctat cttttctgcg
    15121 gcaatcatgt agtcggaggc atcaaaaacc ttatgcgaag aataggcgca attgacaaac
    15181 acctggaaat ccggactcac ggccttcatt agatcataca tcgtcttccg ggcctgaaga
    15241 taggaggtat ccgttaggat agacgctttg ataatcacgt cttccagatt ccttctgccg
    15301 tcaaacagac tcaatagcgc ggcatcgcgg ctggacataa cgccgaaatg caaagcaagg
    15361 tgaggaagat gggcgaacac caccttggtt gtctcctttc taagccgcag gaaaggagac
    15421 aaccccacta catcagttat ctgaaacgga aagcttttat cctgaaacat aagaacacgt
    15481 cgcattgccg caggttccgt taccacctga attcgcattc gatgaacagg cttgcgtgcc
    15541 gcaggtgttt gcgcccttgc attccgccgg aggtgttata ataccaccgc tattggccga
    15601 acaccctgcg ctgctgcacg cgccgttgct acagctggaa ctgaacacac aagcggcaag
    15661 attacatacg atattctcgc aatagctctt cccctccgcc acagcatctt gcctggagta
    15721 cccctgctcc tctagcaaac ttttcagacc caacagcccc tgcaactccg cacccttggt
    15781 gctttggagg aactccttga ccttaccggc ggtttgggga tctttatcgt tgagtaactt
    15841 ctcaattgcg acgatatctc ttccttgcgt tgccttcttc atatgccgcg tctcctttca
    15901 tttcgatttc gacgcaccct cggatacgct ggcgttataa aggacatcag ttaattatgc
    15961 aatgtgaaag cggatgtaag aaaaatcgtc aaaaaaatcc ggcaatcccc cgtccggttc
    16021 tttgcccgtt cgttaacacc atgtcgcttg gaatgtcttc cagctgattt tccgacactg
    16081 gaaagacaga tcaatcctcg gcggtgccct actcgtagca gacgacggtc agcttaggaa
    16141 ctgtttcgat ggtataaatc tccgcgccca gcgccgccag caccgccgac tgatagccgg
    16201 agcccgcgcc gatct
//
LOCUS       DAOPZO010000030        15978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_3915_length_15978_cov_7.593795, whole genome shotgun sequence.
ACCESSION   DAOPZO010000030 DAOPZO010000000
VERSION     DAOPZO010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 15978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15978
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3915_length_15978_cov_7.593795"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..520)
                     /locus_tag="PKI68_03450"
     CDS             complement(164..520)
                     /locus_tag="PKI68_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNX52781.1"
                     /translation="MKKMNKKGFTLVEIMIVVAIIGLLAAIGIPSILNAYSTSQTNAK
                     ARNVAEVEKAKGVLTLPVSSGLPGAMALTDSTADLSSGAAYTNLMSALRIKDKSELKV
                     GDADITIGTLSTKASY"
     gene            complement(798..2174)
                     /locus_tag="PKI68_03455"
     CDS             complement(798..2174)
                     /locus_tag="PKI68_03455"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNX52782.1"
                     /translation="MPRILIVDDEPTILNLLNKILIGQGYDATPASNGEKALQLLQTE
                     RFDLMVSDINMTPINGMELLRKASKSWPDMGVIMLTAYGTVATAVEAMKEGAFDYITK
                     PFKLDELVLTVQRALEYHNAITENKDLKARLEQKDQLGGIVAESPGMRKVCEMIERVA
                     PTSTTVLIYGESGTGKELVARALHHYSPRKDATFMAINCAALPAQLMESEMFGYIKGA
                     FTGANTTKTGLFETAHGGTLFLDEIGSMPIEIQSKLLRVLQDKKIRKVGGSDHTEVDV
                     RIIAASNEKLEKLIEQGKFREDLYYRLSVISIDIPPLRNRPEDVLPLVAHILRKELGP
                     DAELPLIEHEAQSILDNYNWPGNVRELENTIQHALTFAQQGSITKDTLPAKIVNTVEE
                     GIKSGVIANRRDQFKGKSLKAFLHDKEKEFLQRTIESMHGDKEKAANELGISLATLYR
                     KLPSDLKK"
     gene            complement(2305..3564)
                     /locus_tag="PKI68_03460"
     CDS             complement(2305..3564)
                     /locus_tag="PKI68_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNX52783.1"
                     /translation="MTKTNPAEETRTVPWATMMGLLGGRLITISGFLIVLSFVLRQDS
                     IAFYAFIAFAYIITIPYSLWMRNQDRMRRLAPLQFLVDLVFVSGLVYFAGGLNNDFLI
                     LLYPLIILSAGIILPLKQTVQVTVLAIISYVLVILLMSQNILIQYPSAAAARTLVDTS
                     GAMALRIAIFVFFGIASAYVSRRCDYISKKEKQFRDITKIVFENVKTGLLLLDADDRI
                     LMANGLACVLLGRDESELTGKTLASIHMKPSEIKGGDAELRGASDYFRRADGSIFPVS
                     IEDARIALPAEAVPNANAPSGELVDTRILNFNDLSHFLRLQSQTRQLERIKAAANMAK
                     EMAHQIRTPLTGISGIVQLLQINLRNEPGNDAGVSKERNELCQQIVMESIRMDKVIQN
                     FLDYAEFSPKDVRDLIQMDIDQETKRH"
     gene            complement(3577..4587)
                     /locus_tag="PKI68_03465"
     CDS             complement(3577..4587)
                     /locus_tag="PKI68_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNX52784.1"
                     /translation="MYTRQMAAMLSSGMPLVQSIVALEEQTLNKNFRTVLAGVRAKVE
                     GGSMYSDSLDQYPQVFDELYVNMMRAGETGGMLAETSERVATFLEASNRLRAKVKSAM
                     MYPTVVMSVALIISTALIIFIVPTFAKMFGSFGAALPMPTQVLMNVSNFIRNYWYIVL
                     ASAGAIIYSLRRYVKTEKGSYAFDMFRLRFPLLGKLVRKIAITRFASTFAQLLSSGVP
                     IIQAMSIVGLATGNKVIGKAVLDARASVEQGKTISDTLKNNKEFPSMLIHMLSAGEQT
                     GKMEEMLSKLAEFYQEEVDTMLEGLTSMLEPLLMVVIGVMIGGIVLAMFMPIFKMTEV
                     VM"
     gene            complement(4675..6384)
                     /locus_tag="PKI68_03470"
     CDS             complement(4675..6384)
                     /locus_tag="PKI68_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNX52785.1"
                     /translation="MSDKAYKTLLGILFDRFPVKEEALTNARLEADKSGIPLEEVLVA
                     QGTVPAPVMLLAKAEYLEMTPISLEGFQLDASLVEMIPRATWVQLKMLPLCKTGKLLT
                     VAVGDPFNIMGIENLKTQIEYQIFPVIADPKDVLGALEGSAKESAIGLEDILKDIEDD
                     SELEVGHDATSADNLDEMLESAADAPVVRIVNSVLIEALRKHASDIHIEPMEKKIRLR
                     YRVDGILYESPNPPKSVQSAISSRIKIMANLDIAERRIPQDGRFKIKALGKEVDLRVS
                     YLPTVHGEKIVMRILDKTALAPSLDALGLEQRALEDLKFAIAKPHGMLLVTGPTGSGK
                     TTTLYSALQELNTEDVNIITVEDPVEYQLGGINQVQARPEVGLTFASGLRSILRQDPD
                     IVMIGEIRDNETASIAVQAALTGHLVLSTLHTNDAAGAIARMAYMGIEPFLLSSSLVM
                     TQAQRLYRKLCPFCKQQVDLPEKTLRTNHLDPDMFKDVTFFNAKGCPKCSGLGYKGRG
                     ALMEILLLNDEIKAKILETAEASALREVAVRNGMKTLRETGLSRVRDGLTTIDEVLRV
                     TTE"
     gene            6658..7473
                     /locus_tag="PKI68_03475"
     CDS             6658..7473
                     /locus_tag="PKI68_03475"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52786.1"
                     /translation="MSQPVFSVKELEQTAAMLEQILEVMPDDLFTLRALYETSLKLEL
                     PEKAFDCLKRLDDGARVSQNAEIIAFVLNQYASIVDEMPEVQNRIERLRELQMVTDLL
                     RGEDPVKPDAGRKSGIESEMALAWELFQDEQLTQDEYSNVLHDLTEMSSREVSVPVTV
                     LHILHDRNFSRFERLLTYLSQKSGVPIMALGQFSEREDLRSALPLEFISHNGALPFAQ
                     VGGDLLLAVLNPMDKDLVRRAGEISGRHCHAYLVTPQEYDQRLMQIKKALSGA"
     gene            complement(7490..7936)
                     /locus_tag="PKI68_03480"
     CDS             complement(7490..7936)
                     /locus_tag="PKI68_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52787.1"
                     /translation="MNLTSAGEKAPAKPAPAWLRRSLLCLLSGSAVIGAAFVVFLLKP
                     PVKSGPSSFEQQFLSKPSISRSSSGLIEKAALERRNSADSIICQGTLVGKNGKALAII
                     NGQTVSLGMTIDGAKILKIDSSYVVVECAGKKHRLEPGESFTPAKK"
     gene            8086..8562
                     /locus_tag="PKI68_03485"
     CDS             8086..8562
                     /locus_tag="PKI68_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52788.1"
                     /translation="MKKMRCKEISVLEAAIMAGLAAVFFAVATPLVRGAILRQRTAEC
                     ARKIMQAADAFDFYASAAGDYPPNPGVSQKADTAMRGVFSAFNINWWDDQTELGGQWS
                     WFSSGKGSGSVVISGPRISEQQMTRLDALLDDGNLETGAFQRRVGRYHYIIKEHAL"
     gene            8559..9029
                     /locus_tag="PKI68_03490"
     CDS             8559..9029
                     /locus_tag="PKI68_03490"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNX52789.1"
                     /translation="MTKPHHKYGFTLIEIVLAVTIIGLLAAFIVPAAKLALRSRENAQ
                     AASKLRQAVAVFELYRSENGSYPADKNPGEIPPEMTDYFADMKITNWWSSATELGGNW
                     DWDNGYHFKYSVSISAPTKSTVQLTEFDRLIDDGNLATGKFRQSGTQYHYILEQ"
     gene            9035..9451
                     /locus_tag="PKI68_03495"
     CDS             9035..9451
                     /locus_tag="PKI68_03495"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNX52790.1"
                     /translation="MRKRRSRAGMTLVEVMIAMFLVSIAAIIVYTEMLMAYRILMRSR
                     ARLEAQSIAFDYLWEIYNTNNMPDISEQLYSIPTPEWSVLSSNGWINCHILADTNIPP
                     RYWEISVTVWPPTNTLMQIGTNPLARAALWRWRGSR"
     gene            9453..9995
                     /locus_tag="PKI68_03500"
     CDS             9453..9995
                     /locus_tag="PKI68_03500"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNX52791.1"
                     /translation="MNRKKNKQGFTIIEVLLATVIAIMVFAALGLVMTKCFALWKDAT
                     SHWQLAQYARISRERILCGGFADPSGGLLSATNAVTVPDSGWSYLQYITVAGSGAVQQ
                     VRGWNGEADDKSIQLKNGSSSWVYGQASGDAEPVVKVDSFSATVSNDLVTVVYRLKLS
                     AAGKSFTQMQTIHACLVNKE"
     gene            9999..11741
                     /locus_tag="PKI68_03505"
     CDS             9999..11741
                     /locus_tag="PKI68_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52792.1"
                     /translation="MKAAKENQGFVLTGVLMLLLVAALVGGAFLFSARNSFATVDQWR
                     LRDECLFGLQSGLERREYELDQIVRTNNVHDINMFDGLANQSASKTILWTNFYSISPH
                     VVTTLVVTTSGSVIKNLSNDTATITLTNIATASRQEGSVKITRKVREVVEYYYAAAPV
                     LGGDGSVFDNVFFIDNVGLFSGVNADFNGDVYANKDLDLQYSSLKVNGDRYTGGNILD
                     KHEYKCDSWSGYGNNRARPAENTDYNRSNTNTYWPQGYDDNVQSYEMAEKKEMPFIGP
                     LSEYEAYAAANTGFVTQVYAVTSTGGLSNRNTSVAAVWGDGAGENSGIGANDNGCLIL
                     IGTSNNPIRISGVVVARGDIYIKGYYTGAGTLYAGRNIYVLDNLIALNPPTWPHPDSN
                     PEATANSNKTKDFLGLCAKGSMAFGDPGALDVNFLNTPYTGSHATDASDAELGYVSYY
                     AGGVPYFNGNYTVPDGNGSMQRSDGSVRHFYDPMISSTAMSALSPVARLSWIDAVLYA
                     NHLIAGDFQNASLNGAFICRDESVARYGDLTLNWDIRLGSRSKDNLGFSPWLAGMLTR
                     QGDVRKIKWTELAP"
     gene            11738..12124
                     /locus_tag="PKI68_03510"
     CDS             11738..12124
                     /locus_tag="PKI68_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52793.1"
                     /translation="MTRNIFISLVFTVLAGSAQAVRPPDAKAREPQIRTHLQQVRTNY
                     EKKQVERQEEAVRAYNQTRMDIFTPPWMRTGTKADGLSSADPSGAVNAAKAQKRNHRF
                     LVSAVLLILIGAGAGWARYKTREIDE"
     gene            12137..13147
                     /gene="pilM"
                     /locus_tag="PKI68_03515"
     CDS             12137..13147
                     /gene="pilM"
                     /locus_tag="PKI68_03515"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNX52794.1"
                     /translation="MALKQKASTGKEQGRRPTNLLALDFASSGVKAVRLKKTKDHLVL
                     AAIDILPPSGPDAEEKPVLPKPLAAYYTAVCATMENSVVRVFNQILPEEEDIEAVVRE
                     NLSAAKDARVNGLVLSRARGKRESTVLGVAVPEAMIQHYLSLFASGAPAPHSLEISGL
                     AAFSAFLFNRGAQTENQTICLVEAGARCTYVAFLHRNQLQLVNRFDVGGNALQRQVQA
                     SLGVDADMAETILSGGSVDVSVPVRQALNPFIRQLSIYREFIERQNKSALSAVYISGG
                     QSASPYWQTAIKEVLGFIPQVWSPFEKIEVPPEVFPDNLKGQESRFAAAVGAALAGME
                     AV"
     gene            13144..13731
                     /locus_tag="PKI68_03520"
     CDS             13144..13731
                     /locus_tag="PKI68_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52795.1"
                     /translation="MNLQVDLLKKAERRYQGIVSMKVIALGSISVLAGISILVFSLAG
                     ISKMTLRSNLERARHEWERLSPQAAVVRNNQAAIAANGKTLAELEEWSHAGRLSMFGI
                     LRAVQHEIPAQMAVENLYAGLEQASDKDPSYYTLRFSGRARGELTAVEAKRQLNANAE
                     VRGFCSEVKLVSSQRESGENWIFALEGRRQAGGSK"
     gene            13728..14318
                     /locus_tag="PKI68_03525"
     CDS             13728..14318
                     /locus_tag="PKI68_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52796.1"
                     /translation="MMNWSAFTKRQQQMVLVTAVLSVVQVALMFHFLGWTKPAESRGG
                     AAKKELIDLERKLNDARAILRQDEVIHRELDQSAEKLEALTIYTPALSDRYAWAYEYV
                     SRCATLARVDLDNLEEVTYAGDDKKNTARPYEINVSTRCGYNSLVEFLWRLEKGNPLL
                     RIKDVTITAVPGQPQSQQVRIVMQWPASVKIEKGAQ"
     gene            14315..14809
                     /locus_tag="PKI68_03530"
     CDS             14315..14809
                     /locus_tag="PKI68_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52797.1"
                     /translation="MKNSRLPVIGSVVSVLLLGSSLSVAAQATSAITVEAAGKEGKGL
                     SNSISAGSLTAPVSQVSEKWAQEPLRDPFWPVGYFPPDWQQKTSFQSEPDLDGSGWKA
                     ASGKIRISGTSQLDGRAAAIINGELKRIGEQIDVLHEGKTYQWQIVGIDAEGRIQLKK
                     LGIR"
     gene            14826..>15978
                     /locus_tag="PKI68_03535"
     CDS             14826..>15978
                     /locus_tag="PKI68_03535"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNX52798.1"
                     /translation="MKIKTTVITAVLMLVLAIQAQDVAKIFNDLKSPQQQVAAPVSEP
                     APAVIEAAPAVAVTAPASVVGTAPAAAGPAAASAEEVVETIMAKPEPAAGATMKGGLI
                     SVSLKEVELNSVIRLFATLSAANIIVPAMEGDVGTVKVDVNLKDVEWKPALQAILETQ
                     SLELYEKNPGTDVYSVRKKLADAPAAMNVKTFKLNFATVSAVNDMIKNMVPDSGKISL
                     FPARNTIVVQSTPENLAEIQMMIAAIDLPRQQVFIEAKFMELTDSASEKLGIDWQVLG
                     GYGVGVKGIGGTYNYADSKKDGINNSSSVGGNRYTDIAGRPYENMTAEPTTLDSSGNP
                     IIDYPARPGSSDPNATRSFGVTPTTVSKTINGVTSVDDSTVARTLGATMS"
BASE COUNT         3654 a         3996 c         4444 g         3884 t
ORIGIN      
        1 aagcattaac tgatctggag accgtgctca aactgccgga tccttttggt aatcaatgga
       61 aaaaagcgca agaggcaatc cgccgaatca gtaaagcagg aaaagagtaa ggcacaaaaa
      121 aatccccggg aaaacccggg gattttttca aagcaaacca gaattagtag gatgctttgg
      181 ttgacagcgt gccgatcgta atgtcagcgt cgccaacttt aagttcgctc ttgtctttga
      241 tgcgcagagc ggacatcagg ttggtgtaag ccgcaccgct cgacagatcc gctgtggaat
      301 cagtcagtgc catagcgccc ggcaggccgg aagaaacagg aagcgtgagt acgcctttag
      361 ccttttcaac ttcagcaacg ttacgggctt tagcgttggt ctgtgaggtc gagtaggcat
      421 tcagaatgga cggaataccg atcgcggcca agagaccgat gattgctaca acgatcatga
      481 tttcaacgag ggtgaaaccc ttcttattca tctttttcat tttattacct ttatttttcg
      541 tttttatttt ttaatcgccg gtttttcgtt ttgtgacttc aggcgtttgt tttgcgtacc
      601 ggtttaaaaa tcagaagtaa tttaaaagca atacatatgc caagagtagc ttccttctta
      661 tgtcggccat tctaagtgcg ggcactggaa ttgtaaactg ttttttaaaa acagcttata
      721 gccgacatta caagggtgcc tgaatgtgct gtaaaggcat tcctgaccca gaaaaggcag
      781 gctgattttg agaattctca tttttttaaa tcagaaggca gtttgcggta aagggttgca
      841 aggctgattc caagttcatt ggcggccttt tccttgtcac catgcatact ttcaatggtc
      901 cgctggagaa attctttctc tttatcatgc aggaaggctt taagcgattt gcccttaaac
      961 tggtcgcgcc ggtttgcaat gactccggat ttgatgcctt cctcaaccgt gttgacaatt
     1021 ttggccggca gagtgtcttt ggtaatgctg ccttgctgtg cgaaggtgag cgcgtgctgg
     1081 atggtgtttt ccagttcgcg cacattgccg ggccagttgt agttatcaag aatactttgc
     1141 gcttcgtgtt caatcagcgg gagttcggcg tcagggccga gttccttgcg gaggatgtgc
     1201 gccacaagcg gcagaacgtc ttccggccgg ttgcgcaacg gcgggatatc aatactgatg
     1261 acgctgaggc ggtagtaaag gtcttcgcgg aatttaccct gctcgatcag cttttccagt
     1321 ttttcgttgg atgcggcgat aatacgcaca tcgacttctg tgtggtctga gccgccgact
     1381 ttgcggattt ttttatcctg gagcacacgc aggagtttgg attggatttc gatcggcata
     1441 gaaccgattt cgtccagaaa gagggtgccg ccgtgtgcgg tttcaaaaag gcctgttttg
     1501 gttgtattgg ctccggtaaa ggcgccttta atatatccaa acatttcgga ttccatcagc
     1561 tgggcgggca gcgcagcgca gttgatggcc ataaacgtcg cgtctttgcg cgggctgtaa
     1621 tggtgtaatg cccgggcgac gagttctttg ccggttccgc tttcgccgta aatcagtacg
     1681 gtagtggagg tgggcgcaac ccgttcaatc atttcgcaca ctttgcgcat gccggggctt
     1741 tcagcgacaa tgcctccaag ctgatctttc tgttcgaggc gggctttgag atctttgttt
     1801 tcggtgatgg cgttgtgata ttcaagtgcg cgctggacgg tcagaaccag ttcatccagt
     1861 ttgaaaggtt ttgtgatgta atcgaatgcg ccttccttca tggcttcgac tgcggtggca
     1921 actgtgccgt aagcagtcag catgataaca cccatgtccg gccaggattt ggaagcttta
     1981 cgaagcagtt ccatgccgtt gataggggtc atattgatgt cggaaaccat cagatcaaat
     2041 cgctcggtct gaaggagttg cagcgctttt tcgccattgc tggccggcgt tgcatcatat
     2101 ccctgcccga tgagaatctt gttcagcagg tttaaaatgg tcggttcgtc gtctacgatg
     2161 agtatccgcg gcatactagc ctgtcccggg gttgttgttc ttgaaaacga ccggaattta
     2221 tcattatcga gaaaggccgt ctacaggtaa atatagtaat actcgcccaa ttattaagag
     2281 gacccggatg gccggtcatt atgcttaatg ccgtttggtt tcctgatcga tgtccatctg
     2341 gatgaggtcg cggacatctt ttggcgagaa ttcagcgtaa tccagaaagt tttggatgac
     2401 tttgtccatg cggatggatt ccatgacaat ctgctggcaa agctcattgc gctctttgct
     2461 gacgccggca tcgttgccgg gttcgtttcg cagattgatc tgaagcagct ggacgatgcc
     2521 ggagattccg gtgagcgggg tgcggatctg gtgggccatt tctttggcca tgttggcggc
     2581 ggccttaatg cgttcgagct ggcgggtctg gctctgcaga cgcagaaaat gagagaggtc
     2641 gttgaagtta aggatgcgcg tatcaaccaa ttcgccggac ggtgcgttgg cgttcggaac
     2701 agcttccgcc ggcagggcaa tgcgggcatc ttcgatgctg accgggaata tggatccgtc
     2761 cgcacggcgg aaatagtcgg aggctccccg cagctcagcg tcaccgcctt tgatttcaga
     2821 aggtttcatg tggatggatg cgagcgtttt tccggtcagc tcggattcgt cccggcccag
     2881 taatacgcag gcgaggccgt tggccatcag gatccggtcg tcggcatcaa gcagaagcag
     2941 gccggttttg acattttcga aaacgatctt ggtaatgtcc ctgaactgtt tttctttttt
     3001 gctgatgtag tcgcaccggc gggatacata ggcgctggca atgccgaaga aaacgaaaat
     3061 ggcgatccgc agcgccatgg cgccggaagt gtcaaccaga gttcgtgcgg cggcagccga
     3121 aggatactgt atcagaatat tctgcgacat cagcagaatg accagcacat aggaaatgat
     3181 ggctaataca gtgacctgta ccgtttgttt aagcggcaga ataatgccgg cggagagaat
     3241 gatcagggga taaagcaaga tcaggaaatc gttgtttaga ccgccggcga aatagaccag
     3301 ccccgaaaca aaaacaaggt cgaccaggaa ctgcagcgga gccagccgcc gcattcggtc
     3361 ctgattccgc atccagagcg agtaggggat ggttataata taggcgaaag caatgaaagc
     3421 gtagaaggcg atgctgtcct gccgcaatac gaacgacaga acaatcagaa atccgctgat
     3481 ggtgatgagc cgtccgccaa gaagccccat catcgtggcc cagggtacgg tacgtgtttc
     3541 ttctgccggg tttgtttttg tcatagcaga gtccttttac attaccactt cggtcatttt
     3601 gaaaatcggc ataaacatag ccagcacgat accgccgatc attaccccga tcacaaccat
     3661 cagcagcggt tcgagcatgg aggttaagcc ttcgagcatg gtatcgactt cttcctggta
     3721 gaactcggcc agcttgctga gcatctcttc catcttgccg gtctgctctc cggccgagag
     3781 catatgaatc aacatgctgg ggaattcctt gttgtttttg agcgtgtcgg aaatggtttt
     3841 accctgttca acgctggcac gggcgtcgag aacggccttg ccgattacct tgttgccggt
     3901 ggccaggccg acaatgctca tggcctgaat aatcggtacg ccgctggaca gcagctgggc
     3961 gaatgtcgaa gcaaagcggg ttatggcaat tttgcggacc agtttgccca gcagcgggaa
     4021 gcgcaggcgg aacatatcga acgcataact gcctttttcg gttttgacgt agcggcggag
     4081 gctgtaaatg atggctccgg cggaggccag tacaatatac cagtaattgc ggatgaaatt
     4141 gctgacattc atcagtacct gtgtcggcat gggcagggcg gcgccaaagc tgccgaacat
     4201 tttggcgaag gtcggcacaa tgaagataat cagggcggtc gaaataataa gggccacaga
     4261 cataaccacg gtcggataca tcatggcgga tttcaccttg gcgcgcagcc ggttggaggc
     4321 ttccagaaag gtggccacgc gttctgatgt ttccgccagc atcccgccgg tttccccggc
     4381 gcgcatcatg tttacataga gctcgtcgaa cacctgcggg tattgatcca gcgaatccga
     4441 atacatcgaa ccgccttcaa ccttagcccg cactccggcg agcacggtgc ggaaattttt
     4501 attcagagtc tgctcttcca gggcaacgat cgactgcacc agcggcatgc ccgaagagag
     4561 catcgcggcc atctgacggg tgtacatggg taattccttg gtccggattt tccgcgcgcc
     4621 gcttatccgt tttttcccag gtttgtttgc tgctgccata aaggagttcc ttgattattc
     4681 ggtggttact cttaaaacct catcaattgt tgttaatccg tcgcgcactc tggacagacc
     4741 ggtttcgcgc agtgttttca tgccgttacg cacggcgact tcgcgcagcg cgctggcttc
     4801 ggccgtttcg agaatcttcg ccttgatctc gtcgttgagc agaagaattt ccatcagcgc
     4861 tccgcggcct ttgtagccga gtccgctgca cttcgggcag cccttggcgt tgaaaaaggt
     4921 cacatctttg aacatatcgg gatccagatg gtttgtccgc agagtttttt ccggcagatc
     4981 gacctgctgt ttgcagaacg gacagagctt gcggtaaagg cgctgtgcct gcgtcattac
     5041 cagagaggac gaaagcagaa acggctcaat acccatataa gccatacggg cgattgcgcc
     5101 ggctgcatcg ttcgtgtgca gcgtgctcag caccaaatgt ccggtcagtg ccgcctgcac
     5161 cgcaatagag gcggtttcat tatcgcggat ttccccgatc atcacaatgt cggggtcctg
     5221 acgcagaatg gagcgcaggc ctgacgcaaa cgtcagtccc acttccgggc gggcctgtac
     5281 ctgattgatg cctccgagct gatactccac cggatcttca accgtgataa tattcacgtc
     5341 ttcagtgttc agttcctgca gtgccgaata aagtgtggtt gtttttcctg atccggtcgg
     5401 gccggtcacc agtaacattc catgcggctt ggcgatggcg aatttcagat cttccagagc
     5461 cctttgctcc aggccgagcg catccaggct gggcgccagc gcggttttgt ccagaatacg
     5521 cattacgatt ttttcgccgt gtaccgtcgg cagataactc acacgcaggt cgacttcttt
     5581 gcccagcgcc ttaatcttaa aacgaccgtc ctgagggatg cgccgctcgg cgatatcgag
     5641 atttgccata attttgatac gcgaagaaat ggctgactgc acgcttttgg gcggattagg
     5701 actttcgtag agtattccgt ctacgcggta acgcaggcgg attttctttt ccatcggttc
     5761 aatgtgaatg tcgctggcat gtttacggag cgcttcgatc aaaacagaat taacaatgcg
     5821 cacgaccggc gcatcggcgg cgctctccag catttcgtcc agattgtcgg cgcttgttgc
     5881 gtcgtggccg acttccagct cgctgtcatc ttcgatgtcc ttgagaatgt cctccagccc
     5941 gattgcgctt tctttggctg aaccttccaa tgcaccgagt acatctttgg gatccgcgat
     6001 gaccgggaat atctggtatt caatctgcgt tttaagattt tcgatcccca tgatattaaa
     6061 gggatcgccg actgccacgg tgagcagctt gccggtctta cacagcggaa gcattttcag
     6121 ctgcacccag gtggcccgcg gaatcatctc caccagcgac gcatcgagct gaaatccttc
     6181 gaggctgatc ggggtcattt ccagatattc agccttggcc agcagcatca ccggtgcggg
     6241 cacggtgcct tgagcaacca gcacctcttc gagcggaatt ccgcttttat ccgcttcgag
     6301 gcgggcattc gttagagcct cttccttaac cggaaaacgg tcgaagagaa ttcccagcaa
     6361 agttttatag gctttatcgc tcataatttc ttatccgtgc tttgaatgtt cagcacaaat
     6421 ggtgccaagc acaagctcac gatagacttt ccggtcgccg gaaacaagcg tggaagggaa
     6481 tgttgacgga gaaagtggaa taatatccgg tgttaacgca aacctgcact tccagccatt
     6541 cctgaactta acagtttccg gaccaaactc tttttccaag gtttgggaaa atagacggtg
     6601 caactgcggc attaatcatg ctttaatcaa tgcgttattt gttttatagg agaagccatg
     6661 agccaacctg ttttttccgt caaagaactt gagcagaccg cagccatgct cgagcagatt
     6721 ctcgaggtaa tgccggacga tctcttcaca ttgcgtgcac tctacgagac ttcgctcaaa
     6781 cttgaactgc ctgagaaggc tttcgactgc ctgaaacggc tggatgacgg cgcccgcgtt
     6841 tcgcaaaacg cggaaattat tgcctttgtg ctcaaccagt atgcttcgat tgtcgatgag
     6901 atgccggagg tgcagaaccg tattgagcgg ttgcgcgagc ttcagatggt gacagatctg
     6961 ctgaggggcg aggatccggt gaaaccggac gccggacgca aaagcggtat cgaatccgaa
     7021 atggctctgg cctgggagct ctttcaggat gagcagctta cacaggatga atattccaac
     7081 gtactgcatg acctgaccga aatgtcatcg cgtgaagtga gtgttccggt gacggttctg
     7141 catattctgc atgaccgcaa tttcagccgg tttgaacgcc tgctgacgta tctttctcaa
     7201 aaaagcgggg tgccgattat ggcgctggga cagttcagcg agcgcgaaga tctccggtcc
     7261 gctcttcccc tggagtttat aagccacaac ggcgccttgc cgtttgctca ggtcggcggc
     7321 gacctgctgc tggcggttct taatccaatg gacaaggatc tggttcgccg cgccggcgag
     7381 atttcgggcc gtcattgcca cgcttatctg gttactccgc aggaatatga ccagcggctg
     7441 atgcagatta aaaaagcgct gagcggcgca tgatgcgcat ttgcaccttt tatttctttg
     7501 cgggagtgaa gctttcaccg ggttccagcc ggtgtttttt tccggcgcat tcgaccacga
     7561 catagctgct gtcaatcttc agaatcttcg cgccgtcgat ggtcattcct aacgaaacgg
     7621 tctggccgtt gataatcgcc agtgctttgc cgtttttgcc gactaacgta ccctggcaaa
     7681 tgatgctgtc ggccgaattc cggcgttcta aagcagcttt ttcgatcaag ccggacgagc
     7741 tgcgggaaat agatggtttt gacagaaact gttgttcgaa cgaagacggg ccggatttta
     7801 ccggcggctt taacaggaaa acgacaaagg ctgcgccgat aacagcggag ccggacagaa
     7861 ggcagagtaa agaccgccgg agccatgccg gggcgggctt ggcgggggct ttttcgcccg
     7921 cagaagttag attcataacg gctcctttct caattcttgg aataaatacg ctcttcagcc
     7981 gcgaatgcga accatttttt gagatgccaa aacagatcaa cgccggtttc gggaacaccg
     8041 gcaagttgtg gcatatacaa tgctttctaa ttatgcgcga tgactatgaa aaaaatgcgc
     8101 tgcaaggaaa tctctgtgct ggaagcggca attatggccg gtctggcggc tgtttttttt
     8161 gcagttgcca caccgctggt acgcggagca attctccgcc agcgtacggc agaatgcgcc
     8221 cggaaaatca tgcaggcggc ggatgccttt gatttttatg cttcagcggc gggcgactac
     8281 cctccgaatc cgggcgtttc gcaaaaggct gataccgcca tgcgcggtgt tttttctgct
     8341 ttcaatatca actggtggga cgatcaaacc gagctgggcg ggcagtggag ctggttcagc
     8401 agcgggaaag gatccggttc ggttgtcatt tccggccccc ggatttctga acagcagatg
     8461 acgcgccttg acgcattgct ggacgacggc aatctcgaaa caggcgcatt tcagcgtcgc
     8521 gtaggccgtt atcattatat tataaaagaa cacgcgttat gactaagccg catcacaaat
     8581 acggatttac attgatagaa atcgtactgg ccgtgacgat tatcggcctg ctcgcggcgt
     8641 ttatcgttcc ggcagccaag ctcgctctgc gcagccgcga aaatgcacag gcggccagca
     8701 agctgcgcca ggctgtggct gtttttgagc tgtatcgttc tgaaaatgga agttatccgg
     8761 cggacaaaaa tcccggcgaa attcctccgg aaatgactga ttatttcgcg gatatgaaga
     8821 ttacgaactg gtggagcagc gctactgaac tgggcggaaa ttgggactgg gataacggct
     8881 atcactttaa atattccgtt tcgatttcgg ccccgaccaa atcaacggtt cagttgaccg
     8941 aatttgacag gctgattgat gacgggaatc ttgcgacagg aaaattccgt cagtcgggca
     9001 cgcagtacca ctacatcctt gagcaatgac tgctatgaga aaaagaagat ccagagccgg
     9061 catgacactg gtagaggtga tgatcgccat gttcctggtc agcatcgcgg ccatcatcgt
     9121 ctataccgaa atgctgatgg cctaccggat attaatgcgt tcgcgggcgc gccttgaagc
     9181 tcagagcatc gcctttgact acctttggga aatctacaac acgaacaata tgccggatat
     9241 atccgagcag ctttatagca ttcctacccc ggagtggagt gttctctcat ctaatggctg
     9301 gatcaactgt catatattag cggataccaa tattccgcct cgttactggg aaatcagtgt
     9361 aactgtctgg ccgccgacga atacgctgat gcagataggc accaatccgc tggcgagagc
     9421 cgccctttgg cgctggagag ggtctcggta aaatgaaccg gaaaaaaaat aaacagggct
     9481 ttacaatcat cgaagtgttg cttgcaaccg tcattgccat catggttttt gccgcactgg
     9541 ggctggtgat gaccaaatgc ttcgcacttt ggaaagacgc caccagccac tggcagctgg
     9601 cgcagtatgc gcgcatttcc cgcgaacgaa ttttgtgcgg cggctttgcc gatccgtccg
     9661 gagggctgct ttcggcgaca aatgccgtta ccgtccctga ttccggatgg agttatctgc
     9721 aatatattac cgtagccgga tccggtgctg tgcagcaggt tcgcggatgg aacggggagg
     9781 cggatgataa gagtattcag ctgaagaacg gttcttcttc atgggtctat ggacaggctt
     9841 cgggagatgc agaaccagtg gtcaaagtgg actcattttc tgcaacagtc agtaatgatc
     9901 tggttacggt tgtttatcgg ctgaagcttt cagccgccgg aaaatcgttt acccagatgc
     9961 agaccattca cgcctgcctg gtgaataagg agtaggtaat gaaagcggca aaggaaaatc
    10021 agggatttgt gctgacggga gtgctgatgc tcctgcttgt cgccgcgctg gtaggcggcg
    10081 cctttctttt ttctgcgcgc aacagttttg cgacggttga ccagtggcgt cttcgcgacg
    10141 aatgtctttt tggtcttcaa tccggccttg agcgcaggga atatgagctg gatcagattg
    10201 tccggacgaa taatgtgcac gacattaata tgtttgacgg tctggccaat cagtctgcga
    10261 gcaagaccat cctttggacc aatttttaca gtatttcgcc gcatgtggtt accacactcg
    10321 tggtgacgac ctccggttca gtgataaaaa acctttccaa tgatacagcc acgatcactc
    10381 tcacgaatat tgcgacggct tcccgccagg aaggatctgt aaagattacc cgcaaagtac
    10441 gtgaggtggt tgaatattac tatgctgctg caccggttct cggaggggac ggttctgtct
    10501 tcgataacgt ttttttcatt gataacgtcg gactgttttc cggcgtgaat gccgacttta
    10561 acggcgacgt ctatgccaac aaggatctcg atttgcagta ttcttcgctc aaggtaaacg
    10621 gggatcgcta tacaggcggc aatattctcg ataagcacga gtataagtgc gacagctggt
    10681 ccggctacgg aaacaaccgc gcccggccgg ccgaaaatac cgattacaac cggtcgaata
    10741 ccaacaccta ctggccgcag ggatatgacg acaatgtgca gtcttacgaa atggccgaga
    10801 aaaaagaaat gccgtttatc ggcccgctgt ccgaatatga ggcctatgcc gccgccaata
    10861 ccggatttgt gacgcaggtc tatgcggtta catccacagg aggtcttagc aaccgcaaca
    10921 caagcgttgc ggcggtctgg ggcgatggtg ccggtgaaaa ctcggggatc ggtgccaacg
    10981 acaacggctg cctgatcttg atcggcacca gtaataatcc catccgcatc tccggcgttg
    11041 tggtggcgag aggcgatatt tacatcaaag gatactacac cggcgcgggg acgctttatg
    11101 ccggccgcaa tatttatgtt cttgataatc tgattgcact aaatccgccg acctggccgc
    11161 atcctgacag caatccggaa gccacggcca attccaataa aaccaaagat tttcttggct
    11221 tgtgtgctaa aggaagcatg gctttcggtg acccgggggc attggatgtc aactttctca
    11281 atactcctta taccggttct catgccacgg atgccagcga tgcagaactc ggctatgtca
    11341 gctactacgc cggcggtgtt ccgtacttta acggcaatta taccgtgccg gacgggaacg
    11401 gttccatgca gcgttctgat ggaagtgtgc gccactttta cgacccgatg atcagcagta
    11461 cggcaatgag tgcgctgagt cctgtcgccc ggctcagctg gatcgatgcc gtactctatg
    11521 ccaaccattt gatcgccgga gactttcaga atgcatcact gaacggtgcg tttatctgcc
    11581 gcgacgagtc tgtcgcgcga tacggtgatt tgacgctgaa ctgggatatc cgccttggat
    11641 ccagatcaaa agataacctg ggtttttcac catggctggc gggaatgctg acccggcagg
    11701 gagatgtgcg caaaatcaag tggacggagc tggcaccatg acacggaaca tcttcatctc
    11761 gctggttttt acggtactgg cgggttcggc tcaagccgta cggccgccgg acgcaaaggc
    11821 ccgtgaaccg cagatccgca cgcatcttca gcaggttcgc acgaactatg aaaaaaagca
    11881 ggttgagcgg caggaagagg ctgttcgcgc ttataatcaa acccgaatgg atatttttac
    11941 accgccctgg atgcggacag gaacaaaagc cgacggactt tccagcgcgg atccgtccgg
    12001 tgctgtgaat gcggccaaag cgcaaaaaag aaatcatcgc tttttggtgt ccgccgtgct
    12061 tttaattcta ataggtgccg gggctggctg ggcacggtat aaaacacggg aaatcgacga
    12121 atagagattt tattttatgg cgctaaagca gaaagcatct accggtaaag aacaaggcag
    12181 acgcccgact aatctgctcg cgctggattt cgcttccagc ggcgttaaag cggtccgtct
    12241 caaaaaaacc aaagaccatc ttgtgctggc cgccattgat attctgccgc cgtccggccc
    12301 ggatgccgaa gaaaagcccg ttctgccgaa accgctggcc gcctattaca ccgcagtctg
    12361 cgccaccatg gaaaattcgg tagttcgtgt ctttaatcag attcttccgg aagaagagga
    12421 tatcgaagct gtggtgcgcg aaaatctcag tgcggcaaaa gacgcccgtg ttaacggact
    12481 ggttctttcc cgcgcccgcg gtaagcgcga aagcactgtg ctgggcgttg ccgttcctga
    12541 agcaatgatt cagcattatc tctcactctt tgccagtgga gcgccggcgc cgcactctct
    12601 tgagatttcc ggtctggcgg cgttttccgc gtttttgttt aatcgcggag cacagactga
    12661 aaaccagacg atctgtcttg tcgaagccgg cgcacgctgt acctatgtgg ctttcctgca
    12721 tcgcaatcag cttcagctcg tcaaccggtt tgatgtcggc ggcaatgcgc tgcagcggca
    12781 ggttcaggcg tcgctgggag tggatgcaga catggccgag accatccttt ccggcgggtc
    12841 tgtggatgtt tcagttccgg ttcgtcaggc gcttaatcct tttatccggc agctttcaat
    12901 ctaccgcgag tttattgaac ggcagaacaa gagcgcgctt tccgccgttt atatctccgg
    12961 cgggcagtcg gcatcacctt actggcagac ggccatcaaa gaagttcttg gttttattcc
    13021 ccaggtttgg agtccgtttg aaaaaattga agttcccccc gaagtctttc cggacaattt
    13081 gaaagggcag gaatcccgtt tcgcagcagc cgtcggtgcg gcgcttgcgg gaatggaggc
    13141 cgtatgaacc tgcaggttga cctgcttaaa aaagccgagc ggcgctatca gggcattgtc
    13201 agcatgaaag tgatcgcgct gggttcgatc agcgtgctgg cgggcatttc cattctggtg
    13261 ttttcactgg cgggaatttc gaaaatgact ttgagatcca atttagaacg cgcccgtcac
    13321 gaatgggaac gcctgtctcc gcaggctgcg gttgttcgca ataatcaggc ggcgatcgcc
    13381 gccaacggga agacgctggc tgaactcgaa gagtggtcgc atgccggacg gctttccatg
    13441 ttcggcattc tgcgggccgt tcagcacgaa atcccggcgc agatggccgt tgaaaatctg
    13501 tatgccggtc tggagcaggc ctccgataaa gatccgtctt attacaccct gcgcttcagc
    13561 gggcgcgcac ggggcgagct gacagcggtc gaggccaaac ggcagcttaa tgccaatgcc
    13621 gaagtgcgcg gattctgcag tgaagtgaag ctggtttcgt ctcagcgcga atccggcgaa
    13681 aactggatat ttgcgctgga aggccgccgt caggcggggg gttccaaatg atgaactggt
    13741 ccgcttttac caagcgacag cagcagatgg ttcttgtcac cgccgtgctc tcggtggtgc
    13801 aggtcgctct gatgtttcac ttccttggct ggactaaacc ggcggagtcg cgcggcggcg
    13861 cggcgaaaaa agagctgatt gatctggagc ggaagctgaa tgatgcccgc gccattctca
    13921 ggcaggatga agtcatccac cgcgaactgg atcagagtgc cgaaaaactg gaagcgctga
    13981 cgatttatac gcctgctttg tcggaccgtt acgcctgggc gtatgaatat gtttcgcgct
    14041 gtgctacgct ggcccgggtc gatctcgaca acctcgaaga agttacttat gcgggtgacg
    14101 ataaaaaaaa taccgcccgg ccgtatgaaa tcaacgtttc cacccggtgc gggtacaaca
    14161 gtctggttga atttctctgg agacttgaaa agggcaaccc tctgctgcgg atcaaggatg
    14221 tgacgatcac cgcagtaccc ggccagccgc agtctcagca ggttcgcatt gtcatgcagt
    14281 ggccggcctc tgttaaaatc gagaagggag cgcaatgaaa aacagccgtc ttcctgtcat
    14341 cggatctgta gtttccgttt tgctgctggg ctcttccctg tcggtcgctg cacaggcaac
    14401 cagtgccata actgtcgagg cggcggggaa agagggtaaa gggctttcca attcgatatc
    14461 ggccggctcg ctgacggctc cagtttctca ggtttccgaa aaatgggcac aggaaccgct
    14521 tagagatcct ttctggccgg ttggctactt tcctccggac tggcagcaaa agacgtcttt
    14581 tcagagcgaa cctgatctgg acggatcggg ctggaaggcg gcttccggaa aaatacggat
    14641 cagcgggacg agccagctgg acggccgggc tgcggcgatt atcaacgggg agctgaagcg
    14701 gattggtgaa cagattgatg ttttgcatga gggtaaaact tatcagtggc agattgtcgg
    14761 gattgatgcc gaaggacgga ttcaactgaa aaaacttgga atcagataaa atcgggagct
    14821 gatcgatgaa aataaagaca acggtaataa ctgcggtact gatgctggtt ctggcgattc
    14881 aggcccagga tgtggccaag atattcaatg atctgaagtc gccacagcag caagttgctg
    14941 cgcctgtgtc ggaaccggcc ccggcggtca tcgaggcagc tcctgcagtt gccgtaacgg
    15001 ctccggcgtc tgtcgttgga acagcgcccg ccgcggccgg tcccgcggcc gcatctgcgg
    15061 aagaagttgt tgaaaccatt atggcaaagc cggagccggc ggccggcgca acgatgaaag
    15121 gcggactgat ttccgtcagc ctgaaagagg ttgaactcaa cagtgtcatc cgtctgtttg
    15181 cgacgctttc cgctgcaaat attattgttc ccgccatgga aggcgacgtc ggcacggtta
    15241 aagtggatgt aaacctcaaa gatgtggaat ggaagccggc tttgcaggct attctcgaaa
    15301 cccagagtct cgagctctat gaaaaaaatc ccggcaccga tgtttacagt gtgcggaaaa
    15361 agctggccga cgcgccggcg gcgatgaatg taaaaacgtt taaactgaat ttcgcgacgg
    15421 tttctgccgt taatgacatg atcaaaaaca tggttccgga ctccggcaaa atatcgctct
    15481 ttccggcccg caataccatt gtcgtccaga gtacgccgga aaatctggcc gagatacaaa
    15541 tgatgattgc ggcgattgat ctgccgcgtc agcaggtttt tatcgaagcg aagtttatgg
    15601 aactgaccga cagcgcgtct gaaaagctgg ggatcgattg gcaggtgctc ggcggttacg
    15661 gagtcggcgt gaaaggaatc ggcggaactt ataattatgc cgacagcaaa aaggacggta
    15721 tcaataacag ttccagtgtc ggcggaaacc gctataccga tattgccgga agaccgtatg
    15781 agaatatgac agcagagccg acgactttgg attcttccgg aaatcctatc attgattatc
    15841 ctgcccgtcc gggcagttct gatccgaatg ccacgcgttc tttcggtgtt acgccgacca
    15901 ccgtttcgaa aaccatcaat ggggttacat cggtcgatga tagcacggtt gcaaggactt
    15961 tgggtgctac catgagtg
//
LOCUS       DAOPZO010000031        15975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_3916_length_15975_cov_7.746482, whole genome shotgun sequence.
ACCESSION   DAOPZO010000031 DAOPZO010000000
VERSION     DAOPZO010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 15975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15975
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3916_length_15975_cov_7.746482"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PKI68_03540"
     CDS             <1..807
                     /locus_tag="PKI68_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HNX52799.1"
                     /translation="VTSVDDSTVARTLGATMSASDFGLVLAALKETTGAKVVSNPKVI
                     VANEETATIHIGKKKPNVRGTSQTAGDSQRVTTYGLDDKEPYFEDGIKVNVTPTINTS
                     SNITVKIQPTLDRLETDAQAFTAPDGTRFYGKSTKTITTLFSLGDGQTAAIGGLTQTS
                     SDDIERKVPILGSIPLIGRLFSYSAKTRDQVETVIFVTVGLANPEHIDMSTGLPEESS
                     LTMRQNAKMKATRQIKAEELRLIETQETEQAKQTVEKLQNAEQERLQKKK"
     gene            880..6699
                     /locus_tag="PKI68_03545"
     CDS             880..6699
                     /locus_tag="PKI68_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNX52800.1"
                     /translation="MKRSLFPVLIIGVFLAAGISGYLVLHRTGSAVTATSEKAAVSEP
                     VESAVSGSPAQPVPPDVPPQRSSVVKKSGIEEPALQVVAAPGDRFQPVAEAQILDRRV
                     ETVSKDQKKRELLVKAGGKYPFHRVEEMLVKNEGADTYTIASRTEMVADHVLVKLQDG
                     KGEADLRTILGSYGVSTFRKLSLPGHYIVSLKAPTLDAVKEAVSVLSAESAVFAYVEP
                     DYLSHIAAVPNDTRWSELWGMVKINATGAWDFTTGSTNVVVAVLDTGISLAHPDLSPN
                     LWKNTAEVNGAPGVDDDGNGYIDDTSGWDFVNADNIPDDDAGHGTHCAGTIGAVGNNA
                     KGVAGVCWSVRLMALKVLDASGSGREGGWESDIANAIQYASDEGAKIISASLGGYSYS
                     DTARDAIAYANSKGVLFVAAAGNGGADGIGDNNDVTPVYPAGYDLPNIVAVAATDPGD
                     VLAGFSNYGKTSVDLAAPGVNILSTVPGGGYDAYQGTSMATPHVAGAAALLLSAKPAL
                     THLQLKAALLSTVDPVSALAGKTVSGGRLNVQRLISLQDTDGDGIPDDWESANGLNPN
                     NPLDALLDPDGDHLNNRGEYENRCITTNADTDADTLVDGWEVTYGFNPNSPTGGLSRS
                     SSLGGINLSGEAKNIVVAGSYAYVAAGSAGLVILNVSNPEHPVLAGSYDTAGTANDVA
                     VNNGYAYVADGSDGLVIINVSTPTSPFLAGSIDTAGTATGISVQSNYVYLADGTAKTM
                     VVFDVSNPAVPVFKWSDNSLQQAMYDVFACGNSVYIAANANVYRMNISNPAVPVWTTN
                     SANLIGWQLTGIHGNGSVVAAVGGTRGVTIMNDTSIQNRTVLGSYDTAGTASGVFVDG
                     NYVYVADGTNGLVVLDVSTPSAPFLSAHITTAGSASGVFVSGGLTYVTEGAGVEIFSI
                     LSDSDGDGLLDSWEMTWFGNLSRDGTGDFDNDGISDRGEYLAGLNPKNPDQDADGLID
                     GTQEVRIYNTDPRVADTDGDGLADGDEVSRGTNPLLVDTDGDGMKDGWEVTYGFDPLD
                     ILNPGASGDADSDGLTNLEESQNGTAPNNPDTDGDGMKDGWEVAHNLNPLLNDAALDP
                     DSDGLTNLQEYILGTDPHNSDSDNDGMPDGWENSHGFNPLVNDASGDADSDALTNLQE
                     YQQNTNPHNNDTDGDGMPDGWEVSNGLNPLLNDASGDPDSDKLLNIQEYSLCSSSLWQ
                     TVYSSVTGSVATFSFGIPGSTNPQKADSDGDGLTDYYEITTNAASGNLYITNPNSKDT
                     DGDGLPDNWELSQTPVSDPTVPALPGDDSDGDGLTNGEEAALGTQMANRNDPVFVDDD
                     APNDPWPMDPQSSDTNENGTISHPFDAIQEAVNVATNGLTVLVADGTYMGEGNYNINP
                     HGKSIRIISWNNSPTSTVVNSLGYGPVFVMNSGETTNSLLKGLGITVTLSDCSDGECD
                     FEHAIVLSNASPRVENCQIYDGQLNGIYCVGGSSPVIRNCTISRFLNGIWCEGGSSLR
                     IENCKVSESAYRGRFQCDLKSRGIGIYADSNGRLYIGGDSVVSGCQSRGVIVKNCADA
                     VIEDTDVTGCGGGITCDGSSPRIERCIISDNEALNYYMYKSWYKSERVLFPLGESDYI
                     ELTDEDENGGGVLLLRSSSPVMVNCLISGNRTWAEDPDYSGTKLVPDYGLGGGIYIGD
                     GCSPTGVNCTVVDNHANTRGGGLSSFQSPFLRNMIFWGNTCSNVAIVESARIGSPSSP
                     YRNLHCRSGSINIWYSDIQYGYPTAVLSSANDPLFVGGGNYHLSTSNSPCYNKGTFYL
                     APLLDLDGNVRPTALPGRVDMGCYEFGAIPPAPDAAADPDVAAASTGGAAPDPLADSD
                     GDGFSNGAEMTAGTDIYDRNDYFRASSEQSHADGSVLISWQSITGSRYTVQFTGNLAV
                     GTWSNVAGYTDLPGTGAVMSFSEKMPGTVRYYRVLVRQP"
     gene            complement(6739..8187)
                     /locus_tag="PKI68_03550"
     CDS             complement(6739..8187)
                     /locus_tag="PKI68_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNX52801.1"
                     /translation="MNRLDELLKLTVERGASDLHLSANYCPCIRVDGEMQFLNEFPVL
                     TPEANEAMLLAGMPEHNRREFEERWDTDFSYKIDGIGRFRVNAFKDTHGTGGVFRSVP
                     EQVPALEDLGLGFSGVLRELCMHTKGLVIVTGPTGSGKSTTLAAMIDLINRTRKEHII
                     TIEDPIEFLHQSKECLINQREVHRHTRSFAGALRAALREDPDIVLLGEMRDLETTEIA
                     LETAETGHLVLATLHTNTATSTVDRIIDKFPGDRQNQIRTLLANTLIGVVAQTLCKRM
                     DGGRVAAAEILLATSAVRANIRDQKTHQLPTAIQTGYSVGMRSFTDSLFQLVQDGIIT
                     PREAYLKAVDKVSLEQKFSSAGIPLDKTAAEMPVAGAGELDPASDMNSPDRLKEKAWK
                     LATDRNSQARDGGKALELIKRAVSLGAKDAETFMVLAAAQAETGDFRAAIDTVKEALQ
                     VAKKEHNTVCLRELEQQLGYYKKSRPHPGVVA"
     gene            8647..8979
                     /locus_tag="PKI68_03555"
                     /pseudo
     CDS             8647..8979
                     /locus_tag="PKI68_03555"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
     gene            9358..10266
                     /gene="rpoD"
                     /locus_tag="PKI68_03560"
                     /pseudo
     CDS             9358..10266
                     /gene="rpoD"
                     /locus_tag="PKI68_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291595.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
     gene            complement(10338..10763)
                     /locus_tag="PKI68_03565"
     CDS             complement(10338..10763)
                     /locus_tag="PKI68_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNX52802.1"
                     /translation="MKQFAVLFSILMLAAGVAFAEDCAGGVCPLPSAGKAPDKVTTAE
                     LAGLIKAGSVIVIDARPGVTTGLPGAKLLAGQPTAQEAAKVIPSKNSPVVTYCGSTSC
                     FLSENLAKHLKTLGYTNVREYPQGYAGWKQAGNPVVPIQ"
     gene            complement(10827..14582)
                     /locus_tag="PKI68_03570"
     CDS             complement(10827..14582)
                     /locus_tag="PKI68_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HNX52803.1"
                     /translation="MKKQLLTALFILSAGLASAQLDLSAELIARNTLTAAAYSAEQHP
                     DADILLVDDQTRIRYETDGTYRCISDMAFKILTEKGRQEKSSIEIGYSTAYGTMRFVR
                     AEVAKPDGRVIPVDLAAQSRESINQGQMEANIYDPNQKSLKLTIPDLGIGDVLRYTVA
                     SEQTKTVVPGTWGDLFTLEETFPISHAVYEIDAPAARPLARIELKAEIPGTVTSRTEK
                     NGDRIHYIWEVNNVPRMFEEPKMPESYTVAQRLLVSTIPDWETLSKWYWELSRPRLDH
                     VNPAMQDKVRELTAGLTGRQEKIEAIFRFVSQDVRYMGLTIENEAPGYEPHDVSLTFD
                     NRYGVCRDKAALLVSMLNLAGFDACPVLIYVGPKKDPEVPQPWFNHAITAVRNEDGSW
                     QLMDATNENTRDLLPAYLSDRSYLVASPQGETLRTSPVVPPEKNLLTIDVDASLDEAR
                     LITAKAVLSFNGINDTSYRGRLAALKPEERELYFEDLLKQALGAARLTRLEISPKEVR
                     NTTEPLSIALSFEIENALADGKDTALLQVPTLINHFGLFGRVLGDGTGLEKRRYPLNT
                     GFTCGIAETVRLDLGRSGLRPAALPAYETVDSPELYINRAVSSTNALLISRADIMLRT
                     VEFTPEQYLVLKQNLKTSERNARKRVILEHSGFPSKADIATLDETVQYTVFDQHNWQE
                     ERTVRQKVLTYAGKKQASDLKFTFNPAVQRTVLSSATVTSPDGKVHYIDPEKEINVMD
                     AKWAGEAPRYPAEKTMVASLPGVEVGSIIEYRVFSVYRGMPFFSAAEYFSDYNPLVSK
                     KVRIEIPYGLKLRTSEPDRSAVQQRTFSIDGGAAHEWVAKNSAMIKKEDRLPPDWTYK
                     PALLLSCGEISGYAALVEKTLTEAAGKDKAASAKTRELTKGLKTRVQKITALRDFVDR
                     TVREAGPNFAALPLSAVTPADQVLAEGYGNTADRAVLLYSLLDAAGLKPRFVLSSSLP
                     QTEGAGAPVTTALQRSVFDTVLVAVTGDKKETVYLGDSSQYAQLGTLAHSGQPAIDLK
                     SGKLEIPQTGSPSAVETAFTLTLSENGDITLTKKAGLSGTDFEEFHKQFAQFTPEERR
                     REHQKLLSQISQSAEATGELQTAFDTVPGTLEFAARLPSYAVRDGSRLYFNLPEGLGG
                     LLNLKASRRENPFYIKNPVCKSFLYEINLPEGWKPLLVPEAFRTVLPAGAGFVEIKVS
                     VSAGKIFILQQAQLNPAVIPADEYNKLLELNNRLTAPSGRAILLQKL"
     gene            14793..15389
                     /locus_tag="PKI68_03575"
     CDS             14793..15389
                     /locus_tag="PKI68_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52804.1"
                     /translation="MGLFGKVKSVLVVDTAAVLKTKGVRGRAAPRQQLQILRSLSRFV
                     QRENLKVIAVLAGDPLDKAPHNKVFEGVRVRYAKSEEALDKELLKALKQAGSAGVLVT
                     DEVALETRVLRAGGSILRVSTFRKLLDDGIEQNESDRGNNNNNRPQRNRRDRDRDRGP
                     RPPNRNNNQQQQQRPQADETEEQNSGEDTISQMIDLVE"
     gene            complement(15460..>15975)
                     /gene="hpt"
                     /locus_tag="PKI68_03580"
     CDS             complement(15460..>15975)
                     /gene="hpt"
                     /locus_tag="PKI68_03580"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNX52805.1"
                     /translation="FTSDQIRQRVQSMIGEIAGTCEPGNLVMIGILRGSFIFLADLVR
                     GFAQHNLHPRIDFITLSSYGGGTESSGTVRICHDTDEDLRGADVLIVDDILDSGRTLA
                     FAKELFSQRGARSVRTCVLLDKKTRRATDMEADYAGFSIDDVFVVGYGLDYDSHYREL
                     PHIAKVTFLNG"
BASE COUNT         3553 a         4072 c         4731 g         3619 t
ORIGIN      
        1 gttacatcgg tcgatgatag cacggttgca aggactttgg gtgctaccat gagtgcgtcg
       61 gatttcggcc tggtactggc cgctttgaag gaaacgacag gtgccaaggt tgtttccaat
      121 ccgaaggtga ttgtcgcgaa tgaagagact gcgacgattc atatcggaaa gaaaaagccg
      181 aatgtcagag gtacatcgca aacggccggt gattcgcagc gggttacaac gtacggactg
      241 gatgataaag agccctattt cgaagacggg atcaaagtca acgtgactcc gaccatcaat
      301 acttccagca atattaccgt caaaattcag ccgacactcg accggctgga aacggatgcg
      361 caggcgttta cagcgccgga cggtacacgg ttttacggaa agagcacgaa gaccattacg
      421 acactctttt cgctgggaga cggccagact gccgctatcg gcgggttgac acaaacctcg
      481 tcggacgaca tcgagcgcaa ggttccgatc ctcggaagta ttccgctgat cgggcgcctt
      541 ttctcgtaca gtgccaagac tcgcgatcag gtcgaaaccg tcatcttcgt aacggtcggc
      601 ctggcgaatc cggaacacat cgacatgtca accggactgc ccgaagagtc ttcgttgacg
      661 atgcggcaga atgcaaaaat gaaggcgacc cggcagatta aagccgaaga attgcgtttg
      721 atcgaaactc aggaaactga acaagcgaag caaacagttg aaaaactgca aaatgccgag
      781 caggaacggc ttcagaaaaa gaaataagat actttcgtcg cgggaatatc taaccgtccc
      841 gccaaccatc ttattccgga acaaacagac aggtgtttta tgaaaagatc gctgtttcca
      901 gtactgatca tcggggtgtt tctggcggcc gggatttccg gctatctggt tttacaccgc
      961 accggttctg ccgttacagc gacgtccgaa aaagctgctg tttctgaacc cgttgaatct
     1021 gctgtttccg gttctccggc tcaaccggtg ccgccggacg tgccgccgca gcgttcctct
     1081 gttgtgaaaa aatccggtat tgaggaaccc gcattgcagg tggtcgcggc tcccggcgac
     1141 cggtttcagc ctgtcgccga agcgcagatt cttgaccggc gggttgaaac ggtttccaag
     1201 gatcagaaga aacgcgaact gctggtcaaa gccggcggta aatatccgtt ccaccgcgtg
     1261 gaagaaatgc tggtgaagaa cgaaggggca gatacctaca cgattgcttc ccgcaccgaa
     1321 atggtggcgg atcatgtgct ggttaagctg caggatggaa agggtgaggc cgatctccgg
     1381 acgatactcg gaagctatgg tgtttcgact tttcgcaagc tgtcgctgcc gggacattac
     1441 atcgtcagcc tgaaagctcc gacgctggat gctgtgaaag aagcggtttc tgtcctttct
     1501 gcggaatctg ccgtctttgc ttatgttgag ccggattatc tttcacatat tgccgcggtt
     1561 ccgaacgata cacgctggag tgagctgtgg ggcatggtca aaattaatgc caccggcgcg
     1621 tgggatttca cgaccggcag caccaatgtg gttgtcgcgg tgctggatac agggatcagt
     1681 ctggcccatc ctgatttatc gcccaatctt tggaaaaaca cggctgaagt caacggcgct
     1741 cccggggtgg atgatgacgg caacggatac atcgacgaca ccagtggctg ggattttgtg
     1801 aacgctgaca atataccgga cgatgatgcc gggcacggaa cacattgcgc cgggacaatc
     1861 ggggctgttg gaaataatgc caagggggtc gccggcgtct gctggagtgt caggctgatg
     1921 gccttgaagg ttcttgatgc cagtggatcc gggagagaag gcggatggga atctgatatt
     1981 gccaatgcca tccagtatgc ttccgatgaa ggggcgaaaa tcatttccgc ttctcttggc
     2041 ggatattcgt acagtgatac ggcccgcgat gcaattgcct acgccaacag taaaggcgtt
     2101 ctttttgttg ctgccgccgg aaatggcggt gcggacggga ttggcgacaa taacgatgta
     2161 accccggttt atcctgcggg ctatgatctt cccaatatcg tcgccgttgc cgcgaccgac
     2221 ccgggtgatg ttctggccgg cttctcaaat tacgggaaga cttctgttga tctggctgca
     2281 cccggcgtga atatcctcag cacagttccg ggcggcggtt atgatgccta tcagggcaca
     2341 tcgatggcga cgccgcatgt cgcgggcgcc gcggctctgc ttctgagtgc caagcctgca
     2401 ctgacgcatc ttcagctcaa agcggctctt ctcagtacgg ttgacccggt ttccgcgctg
     2461 gccggtaaaa ccgtttccgg cgggcgactg aatgttcaac gattgatttc gcttcaggac
     2521 acggatggcg atggaatacc ggacgactgg gaatcggcga acggactgaa cccgaacaat
     2581 ccgctggatg ctttgctgga tccggatgga gaccatttga ataaccgcgg tgaatatgag
     2641 aatcgctgta tcacaaccaa cgcagatacc gatgcagaca ctctggtgga cggctgggaa
     2701 gtgacgtacg gctttaaccc gaattccccg accggcggac tttcccgttc ctccagtctg
     2761 ggtggaatca acctgtccgg tgaggcgaaa aatatcgtgg tggccggttc ctatgcctat
     2821 gtagccgccg gttccgccgg attagttatt ttaaacgttt caaatccgga acatccggtg
     2881 ctggccggct cgtatgatac ggccggaact gcgaatgatg tcgcggtgaa caacggctac
     2941 gcgtatgtag cggacggctc cgacggactg gtcattatta atgtctccac tccgacttct
     3001 ccctttctgg cgggaagtat cgacacggct ggtacggcaa cggggatttc cgtgcagagc
     3061 aattatgtat atctcgccga tggaaccgct aaaacaatgg tcgtttttga tgtgtcgaat
     3121 ccggcggtgc ccgtatttaa gtggagtgat aattcattac agcaggcgat gtatgatgtt
     3181 tttgcctgcg ggaattcggt gtatatcgcg gcaaatgcca atgtgtaccg gatgaacata
     3241 tctaatccgg cagttccggt atggaccact aattcggcta atcttattgg ctggcagctg
     3301 acgggaattc atggcaacgg atccgtggtt gctgcagttg gcggtacccg cggcgtgacg
     3361 attatgaacg acaccagcat ccaaaacaga actgttcttg gaagctatga tactgccggg
     3421 acggcttccg gcgtgtttgt tgacggaaat tatgtgtatg tggccgacgg aacaaacggt
     3481 ctggtggttc ttgatgtcag tacaccgtct gctcccttcc tgtctgcgca tattacaacg
     3541 gcaggttccg cttccggagt gtttgtcagc ggcgggctca cttatgtgac agaaggtgca
     3601 ggggttgaga tcttctccat tctgtcggat tccgacggag acggcctgct ggatagctgg
     3661 gaaatgacct ggttcggcaa cctgtcgagg gatggaacag gggattttga caacgacggg
     3721 atatccgacc ggggcgaata tctcgccgga ctgaatccga agaaccccga ccaggatgcg
     3781 gacggactga tcgacggaac gcaggaagtc cggatttata ataccgatcc gcgtgttgcc
     3841 gatacggatg gagacggact cgccgatggt gatgaagttt cccggggaac aaacccgctg
     3901 cttgttgaca ccgatggcga cggcatgaag gatggctggg aagtaaccta cggttttgat
     3961 ccccttgata tcctgaatcc cggcgcgtcc ggcgatgccg acagcgatgg gttgaccaat
     4021 cttgaagaat ctcagaacgg cacggctccg aacaatccgg atacagacgg cgacggcatg
     4081 aaggacggct gggaagtggc gcataacctg aacccgctcc tgaatgatgc ggcgctggat
     4141 cccgacagtg acggcctgac caatctccag gaatacatac tgggaaccga tccgcataac
     4201 agcgacagtg ataacgacgg catgccggac ggctgggaaa attctcacgg tttcaatccg
     4261 ctcgtaaacg atgcatccgg cgatgccgac agcgacgcgc tgaccaatct gcaggaatat
     4321 cagcagaaca cgaatcctca taataacgat acggatggcg acggtatgcc tgacggctgg
     4381 gaggtttcca acggacttaa tccgttgctg aacgacgctt ccggcgatcc ggattccgac
     4441 aaacttctca atattcagga atacagtctg tgttcgtcga gcctctggca gacggtttat
     4501 tcatccgtta ccggatcggt tgccaccttc agcttcggta tacccggttc aacgaaccct
     4561 caaaaagcgg acagcgacgg cgatggcctg accgactatt acgaaatcac caccaacgcg
     4621 gcatccggca atctctacat taccaatccg aacagcaagg acaccgatgg cgacggtctc
     4681 ccggacaact gggagctctc ccagactcct gtcagcgatc cgacggtgcc cgcgcttccg
     4741 ggtgacgaca gtgacggtga cggtctgact aacggcgaag aagcggcgct cggtacgcaa
     4801 atggcgaatc gaaacgatcc ggtttttgtg gacgacgatg cgccgaatga tccctggcca
     4861 atggatccgc agagcagtga taccaatgag aacggcacga tcagccatcc tttcgatgcg
     4921 attcaggagg cggtcaatgt agcgaccaac ggcctgactg tattggtggc cgacggcacc
     4981 tatatgggcg aggggaacta caacatcaac ccgcatggta aatcgattcg aataatttca
     5041 tggaacaaca gcccgacaag caccgttgtt aacagtcttg gatacgggcc ggtatttgta
     5101 atgaacagcg gtgaaacgac caattccctg ctgaagggat tgggtattac agttaccttg
     5161 agtgactgtt cggatggaga atgtgacttc gagcatgcga ttgttctgag caatgccagt
     5221 ccacgggtcg aaaactgtca gatctatgat ggtcagctga acggaattta ttgtgttggc
     5281 ggatcaagtc cggtaatccg caactgtaca atttcaagat ttctcaatgg tatttggtgc
     5341 gaaggagggt cttcactccg aatcgaaaac tgtaaagttt ctgaaagcgc ttaccgtggc
     5401 aggtttcaat gtgatctcaa gagccgtggt attgggattt atgcggattc aaacggaaga
     5461 ctttatatcg gcggcgattc tgtggtcagt gggtgtcaga gtcgtggtgt tattgtcaaa
     5521 aattgtgcgg atgcggttat tgaggatacc gatgtgacag gatgcggcgg gggtattacc
     5581 tgtgatggaa gcagcccgcg tatagaacgc tgtataattt ccgacaatga ggcactgaac
     5641 tattacatgt acaagtcttg gtataagtcc gagagagtat tgtttcctct gggagagtct
     5701 gactatatag agcttaccga tgaggacgaa aacggcggcg gagttctgtt gctgcgcagc
     5761 agttctccgg taatggttaa ctgtctgatt tccggtaacc ggacctgggc ggaagatcca
     5821 gactacagcg gcaccaagct ggttcccgac tacggactgg gtggcggaat ttatatcggt
     5881 gacgggtgtt cgccgacggg ggtcaattgt actgtagtcg ataaccatgc gaatacacgt
     5941 ggcggaggct tgagttcatt ccagagcccg tttttacgca acatgatctt ctggggtaat
     6001 acatgctcaa atgtagccat tgtcgagagc gcccggattg gctcgccgag ttcaccgtat
     6061 agaaatcttc actgccggtc aggaagtatc aatatctggt acagcgatat ccagtatggc
     6121 tatcctacgg ccgtactttc ctctgctaat gatccgctct ttgtcggcgg agggaattac
     6181 catctttcga cgtccaattc gccctgttac aacaagggaa cattctatct cgcaccgctg
     6241 ctcgacctgg atggaaatgt ccgtccgacc gctttgccgg gccgtgttga tatgggctgt
     6301 tatgaattcg gcgccatacc gcctgcgccg gatgcggcgg cagatccgga tgttgccgct
     6361 gcatccaccg gcggtgccgc tccggatccg ttagctgatt ccgacggcga cggattcagc
     6421 aacggcgctg aaatgacagc cggaacagat atctatgacc gcaacgacta tttccgcgct
     6481 tccagcgaac agagtcatgc ggacggatcc gtgctgattt catggcagtc aattaccggc
     6541 agccggtaca ccgtacagtt taccggaaat ctggcggtcg gaacgtggag caatgtagcc
     6601 ggatataccg atcttcccgg taccggagcg gtgatgagct tctcggaaaa aatgccggga
     6661 accgtgcgtt actaccgtgt gctggttcgg cagccgtaat cattccgatg tccggcagaa
     6721 tccggacatc ggagaaggtc aggcgacaac gccgggatgg gggcgtgatt ttttataata
     6781 accgagctgc tgttccagtt cgcgcaggca gaccgtgttg tgttcttttt tcgcgacctg
     6841 caaagcttct tttactgtgt caattgccgc tctgaaatcg ccggtttccg cctgtgcggc
     6901 ggccaggacc ataaaggttt ctgcgtcttt agcgcccagg ctgacggccc gtttgatcag
     6961 ttcaagagct ttgccgccgt cacgcgcctg tgagttgcgg tccgtcgcca gtttccatgc
     7021 tttttcttta agccggtccg gcgagttcat atcggatgcc ggatccagtt ctccggcgcc
     7081 ggcgaccggc atctccgctg ccgttttgtc cagcggaatt cccgccgagg agaatttctg
     7141 ttcaagagag actttatcaa cggcttttaa ataggcttcg cggggagtaa tgatgccgtc
     7201 ctgtaccagc tgaaacaggc tgtcggtaaa ggatctcatg ccgacgctgt agccggtctg
     7261 aatggcggtt ggcagctggt gggttttctg gtcgcggata ttcgcgcgta ccgccgaggt
     7321 tgccagcaga atttcggcgg cggcaacccg gccgccatcc atccgcttgc aaagagtctg
     7381 tgcgacgacg ccgatcaggg tattggcgag cagggtgcgg atctggttct ggcggtctcc
     7441 gggaaactta tcaatgatgc ggtcaaccgt agaggtggcg gtgttggtgt gcagtgtggc
     7501 cagcaccaga tgtccggttt cggccgtttc gagcgcgatc tcggtggttt ccaggtcgcg
     7561 catttccccg agcaggacaa tgtccgggtc ttcgcgcaat gccgcgcgca gagcacccgc
     7621 aaaactgcgg gtatgccgat gtacttcgcg ctggttgatc agacattcct tggattgatg
     7681 caagaattca atcggatctt cgatggtgat gatgtgttct ttgcgcgtcc ggttgatcag
     7741 gtcaatcatt gcggcaaggg ttgtcgattt tccgctgccg gtcggtccgg tgacgatgac
     7801 cagtcctttg gtgtgcatgc agagttcgcg caggacgccg gagaagccga gaccgaggtc
     7861 ttccagcgcc ggcacctgtt cgggaacgga acggaatacg ccgcctgtac catgggtatc
     7921 tttgaaggcg ttgacgcgaa accggccgat gccgtcaatc ttgtagctga agtcggtgtc
     7981 ccagcgctct tcaaactcgc gccggttgtg ttccggcatg ccggccagca gcatggcttc
     8041 gtttgcttca ggggtaagga cagggaactc attcaaaaac tgcatctcgc cgtcgacgcg
     8101 gatgcagggg cagtaattgg cggaaaggtg cagatcgctg gcgccgcgtt ctacggtgag
     8161 tttcagcagt tcatcaagtc ggttcacggt atgctcctgt tttgcggggc gtttcgttgt
     8221 aacgttttat aaaatagcag gatgcgttcc aaaacagtaa taaaaactgc cggttttcgc
     8281 tccgggaaaa accttgacgg aactggctct ttgctgatta atgcatgcct ttcgcgtata
     8341 atgggatttt tgtatctttc tcaggaattg gagtttttca tggttaagaa gaaagcagcg
     8401 gcggcaaaga aaacagagaa gaagaaaacg atcccggtcg ttaaaaagac accagaggct
     8461 aaaaaggccc cggtcaaaaa agaaccggcg aaacctgttg tgaaaaaagc ccctgtggag
     8521 ctcaaaaagg ttccggttaa aaaggatcgg ctgcctgcaa tctccatacc gaatgaggaa
     8581 atcgctgaaa gtcccgatct tgatgcaatg gaagctccgc agcttgggga tgctcccgtt
     8641 tcagtgatgc ttcgtgagga acgcaatgag aagatcaaag aactgatcgc gctcgcttcc
     8701 gaacacggct ttttgacgca tgaagatatt gaagaagcac tgcccgaaga ccttgttgtt
     8761 cccgaagacg ccgaaaaagt cgttgccttg ctgaaaagca tggaagtgga aatcatccgc
     8821 tcagacgatg tcgaagaatt taaagcgcgc atggagaaag aagagcgcga aaaacagagc
     8881 gcccgcagcg acgtgctcga cgacccggtt cgcatgtatc tcaagcagat ggggcaggtg
     8941 ccgctgctca cccgcgaaca ggaagtggag atttccaaac gcattgaaga agctgaactc
     9001 aataccacca gcctgttcaa ccggatcggc tgcgcgaccg aagcgtatct gggtgtcatt
     9061 gagaaaatcg aaaccggcaa acagcgcttc gaccgtattg tcagcgacaa gcatgtggaa
     9121 tgtcgtgaag actattttaa tgcactgccc aaggtcctgc agggcatccg ccggaatcag
     9181 gagctggcca gtaaatattt tctcgatgtg atccgcgcca agagcaaagc ggagcgcgag
     9241 aaaaaccaga aactgttcga aaacgcacag gcgcgccttt cggaatattt cagccagctg
     9301 cacttcaagc agaaagccat tgaggatatc gttccgcagg ttgattcgta ctacagggaa
     9361 gtgcacgaac tgctgaccga actggctaaa ctcgataaga gccgctccaa gacagccgat
     9421 gaaaaaatcc gggaggtccg caaacggctg cgcgaactcg aagaaattgt gcacatggaa
     9481 gccgagccct atgtcgaaca ccaccgtctg ctgaagcagt ggctgcgcaa aggacttcag
     9541 gcgaagacgg aaatgatcga ggccaacctc cgtcttgtaa tcagtattgc caaaaaatac
     9601 accaaccgcg gcctttcttt cctcgacctg attcaggaag gcaacatggg tctgatgaaa
     9661 gccgttgaga aatttgaata ccgccgcggt tataaattct ccacgtatgc cacatggtgg
     9721 atccgccagg ccatcacgcg ctcaatcgct gaccaggccc gtacgatccg cattccggtg
     9781 catatgattg aaaccatcaa caaactgctt cgcgtccaga aacagctctt gcaggaattc
     9841 ggccgcgaag ccgcgccgga agaactggca gatgaaatgg aactcccggt cgaacgcgta
     9901 cgcgcgatcc tgaaaatcgc ccagcagccg atttctctgc aggcgccgat cggcgacagc
     9961 gacgacacca gcttcggcga tttcatcgaa gacaaagctg cggaaaaccc gtcagaaatg
    10021 acggcgttca gtctgttgcg cgaaaagatc ggcgacgttc tcgaaacgct cacagcgcgc
    10081 gaacaggaag ttctcacgct gcggttcggt ctggccgacg gttacccccg gacgcttgaa
    10141 gaggtcggac ggcagtttaa cgttacgcgt gaacgtatcc gccagattga agcgaaagca
    10201 ttacgcaaaa tgcgtcaccc gacgcgtatc cggaagctcg aaggcttcct tgagcagggc
    10261 cggtaagatc ccggcatgat gaaaagaaaa cccgttttcc ggattccggg aaacgggttt
    10321 tttgcgaaac ggtatgttta ctgaatcgga acaaccgggt tgccggcctg tttccagccg
    10381 gcatagccct gcggatattc ccgcacattg gtatagccga gagtcttcag gtgtttcgcc
    10441 agattttcac tcaggaagca gctggtgctt ccgcagtagg tgacgaccgg cgaatttttt
    10501 gacgggatga ctttggccgc ttcttgagct gtcggttgcc cggcgagaag tttggctccg
    10561 ggaagtccgg ttgttacgcc tggccgcgca tcaatcacga taacggagcc ggccttaatc
    10621 aatccggcga gctcggccgt cgttacttta tccggggctt tgccggcgct cggcaacggg
    10681 catactccgc ctgcgcagtc ttcagcgaac gccactcctg ccgcaagcat gagaatagaa
    10741 aacaacactg cgaactgttt cataatgcct cctgtttctt ccggaaaggt aacattttct
    10801 ctggtcggaa gccagatttc gtcttctcag agtttctgca aaagaatcgc gcgtccggac
    10861 ggtgcggtca gtctgttgtt gagctccagc agtttgttgt attcgtcggc gggaatgacg
    10921 gccgggttca gctgcgcctg ctgcaggatg aaaatttttc cggcggatac ggaaaccttt
    10981 atttccacga atcccgcgcc ggcgggcagg acggtacgga atgcttcagg aacaaggaga
    11041 ggtttccagc cttcgggcag gttgatttca tagaggaacg atttgcaaac cgggttttta
    11101 atgtaaaagg gattttcgcg gcggctggct ttaaggttca gtaagccgcc gagtccttcc
    11161 ggcagattga agtacagccg gctgccgtcg cgcacggcgt atgacggcag gcgggccgca
    11221 aactccagtg ttcccggaac ggtgtcgaag gccgtctgaa gttctcccgt tgcttcagcc
    11281 gactgggaaa tctgacttag cagtttttgg tgctcgcgcc gccgttcctc gggtgtgaac
    11341 tgggcgaatt gtttatgaaa ttcttcaaaa tcagttccgg agaggccggc ttttttcgtc
    11401 agagtaatgt cgccgttttc ggaaagcgta agtgtaaagg cggtttccac tgcagaggga
    11461 gatccggtct gcggaatttc gagtttaccg cttttcaggt caatcgccgg ctggccggag
    11521 tgggccaatg tgcccagttg tgcgtactgg ctgctgtcgc cgaggtaaac ggtttctttt
    11581 ttatcgccgg ttacagcaac aagcaccgtg tcaaaaacgc tgcgctgcag cgcggtggtt
    11641 accggtgcgc ccgcgccttc agtttgcggc aggctggagg aaaggacgaa gcgcggtttg
    11701 agtccggcgg cgtcgagcag tgaatagagc aggacagcgc ggtcggcagt gttgccgtag
    11761 ccttcggcca gcacttgatc ggcgggtgtg acggcggaga gaggcagggc ggcgaagttc
    11821 ggcccggctt cgcggacggt gcggtcaacg aaatcgcgca gcgcagtaat tttttgaacc
    11881 cgggttttaa ggcctttggt cagttcacgc gttttcgcgg aggccgcttt atcttttccg
    11941 gcagcttcag tcagtgtttt ttccaccagt gccgcataac cggatatttc gccgcaagag
    12001 agcagcagtg ccggtttgta agtccagtcc ggaggcaggc ggtcttcttt tttgatcatg
    12061 gcgctgtttt tagcgaccca ttcgtgcgct gccccgccgt ctatgctaaa ggtgcgctgc
    12121 tgaacggccg accggtcggg ctcgctggtt ctcagtttca ggccgtaggg gatctcgatg
    12181 cgaacctttt tactgacgag aggattgtaa tcggaaaaat attccgctgc ggagaaaaac
    12241 ggcattccgc gataaacgct gaagacgcga tattcaataa tgctgccgac ttcgactccc
    12301 ggcaggctgg caaccatggt tttttccgcc ggatagcggg gtgcttcgcc ggcccatttg
    12361 gcgtccatga cgttgatttc cttttcggga tcaatgtaat gcactttgcc gtccggggaa
    12421 gtgacggttg cactgctgag gacagtgcgc tgcacggcag ggttgaatgt aaacttcaga
    12481 tcggacgcct gttttttacc ggcgtaggtg agtactttct gccggacggt tcgttcttcc
    12541 tgccagttgt gctgatcaaa aacagtatat tgaacggttt cgtcgagcgt ggcgatgtcc
    12601 gctttggacg ggaagccgct gtgttcaaga ataacccgtt tgcgggcgtt gcgttccgag
    12661 gttttcagat tttgtttgag caccagatat tgttcgggag tgaactcgac ggtgcgcagc
    12721 ataatgtcgg cgcggctgat gagcagtgcg ttggtcgagc tgacagcgcg gttgatgtag
    12781 agctccggcg agtcgacggt ttcataggca ggcagtgcgg cggggcgcag tccgctccgt
    12841 ccgaggtcga gacggacggt ttcggcgata ccgcaggtga agccggtatt cagcggatag
    12901 cggcgttttt ccagtccggt tccgtcgccg agcacacgtc cgaaaaggcc gaagtggtta
    12961 atgagtgtcg gcacctgtaa tagcgcggtg tcttttccgt cggccagcgc attttcgatt
    13021 tcaaaactca aggcgatgga gagcggctcg gtggtattgc gcacttcttt cgggctgatt
    13081 tcgagccggg tcaggcgggc cgcgccgagc gcctgcttga ggaggtcttc gaaatagagc
    13141 tcgcgttcct cgggtttgag tgcggcgaga cgtccgcggt aagaggtgtc gttgatgccg
    13201 ttgaacgaga gaaccgcctt tgccgtgata agccgggctt cgtcgagaga tgcgtcgaca
    13261 tcaatggtga gcaggttttt ttcgggcgga acaaccggcg atgtccgcag ggtttcacct
    13321 tgcgggctgg cgaccagata gctccggtcg gaaaggtagg cgggaagcag gtcgcgcgta
    13381 ttttcattgg tggcgtccat cagctgccag ctgccgtctt cgttgcggac ggcggtgatg
    13441 gcgtggttga accacggctg cgggacttcc ggatcttttt tcgggccgac gtagatcaga
    13501 acagggcagg cgtcgaatcc ggcgaggttc agcatggaaa cgagcagggc ggctttatcg
    13561 cggcagactc cgtagcggtt atcaaaagtc agcgaaacat catgcggctc gtagccgggc
    13621 gcttcgtttt cgatggtgag gcccatgtag cgcacatcct gcgaaacgaa acggaagatg
    13681 gcttcgattt tttcctgccg gccggtgagg ccggcggtca gctcgcggac tttgtcctgc
    13741 atggccgggt ttacatggtc gaggcgcggc ctggaaagtt cccagtacca tttggacagc
    13801 gtttcccagt cggggattgt gctgaccagc aggcgctgcg cgacggtata gctttccggc
    13861 attttcggtt cttcgaacat tcgcggtacg ttgttcactt cccagatata atggatgcgg
    13921 tcgccgttct tttcggtccg gctggtgacg gtgccgggga tttccgcctt gagttcaatg
    13981 cgcgccagcg gccgggctgc cggggcgtcg atttcataga cggcatggct gatcggaaaa
    14041 gtttcttcga gcgtgaaaag gtcgccccag gttccgggga caacggtttt tgtctgttcg
    14101 ctggcgacgg tgtagcgcag gacgtcgcca atgccgaggt cgggtatggt cagtttgagc
    14161 gatttttgat tggggtcata aatattggct tccatctgtc cctgattgat cgattcgcgg
    14221 ctctgggcgg caagatctac cgggatgacg cgtccgtccg gcttggccac ttcggcgcgc
    14281 acgaagcgca tggtgccgta ggccgtgctg tagccgatct cgatgctgga tttttcctgc
    14341 cggccttttt cggtcaggat tttaaaggcc atgtcgctga tgcagcggta ggtcccgtcg
    14401 gtttcgtagc ggatgcgggt ctggtcgtcg accagcagta tatcggcgtc cggatgctgt
    14461 tccgcggagt atgcggctgc tgtaagtgta ttgcgggcaa ttaattctgc cgacagatca
    14521 agctgtgcgg aggccagccc ggcactcaga atgaataaag cggtcaacaa ctgttttttc
    14581 ataagactcc ggagttttaa ggacagacat gttagcggca gacgccgttc aatacaatct
    14641 ctgccggact gcaaaaacgg cttggaatcc ggacttgctc cgaaggcgcg gctccggtac
    14701 cttccttcgc cttacgaaac gcgtcgccag cccattctgt tgtccgattg aaacggcgcc
    14761 gcagtaaccc tgaactagag aaaggaagaa ccatggggct cttcggaaaa gtaaaaagcg
    14821 tactggtggt tgatacggcc gccgtactca agaccaaagg tgtgcgcgga cgtgccgcgc
    14881 cgcgtcagca actccagatt ctgcgctcac tgtcccgttt cgtgcagcgc gaaaacctta
    14941 aagtcatcgc tgtactggcc ggtgatccgc ttgataaagc gccgcacaac aaagtatttg
    15001 aaggtgtgcg cgtgcggtat gccaagtcgg aagaggcgct ggataaagaa ctgctcaaag
    15061 ctctcaagca ggccggctcc gccggtgtgc tggtgacgga tgaagtcgcg cttgaaaccc
    15121 gtgttcttcg cgccggcggc agcattctgc gtgtatcgac attccgcaag ctgctggatg
    15181 acggcattga acagaacgaa agcgacagag gaaataataa caacaaccgc ccgcagcgca
    15241 accgccgtga ccgcgatcgt gaccgcggtc cgcgtccgcc gaaccgcaac aacaaccagc
    15301 agcagcaaca gcggccgcag gctgatgaaa cggaagagca gaacagcggt gaggatacga
    15361 tcagtcagat gattgatctg gttgagtaaa atatcccgca gcaaacgcta aaacggcttt
    15421 ttcccgagtc ccgggaaaga gccgtttttg tttagaggat cagccgttca ggaacgtgac
    15481 tttcgcgatg tggggcagtt cgcggtagtg actgtcatag tcgaggccgt agccgaccac
    15541 aaagacatcg tcgatggaaa agccggcgta atcagcctcc atgtctgtgg cgcgtcgggt
    15601 ctttttatcc agcagtacac aggttcgcac ggagcgggcg ccgcgctgag aaaaaagttc
    15661 cttggcaaac gcgagcgttc gtccgctatc gagaatatcg tctacgatca gcacgtcggc
    15721 accgcgcaga tcttcgtccg tatcgtgaca gatccggacc gttccgcttg actcggttcc
    15781 gccgccgtag cttgagagcg taatgaaatc gatgcgcggg tgcagattgt gctgcgcgaa
    15841 accgcgcacg agatcggcca gaaagatgaa gctgccccgc agaatgccga tcatcaccag
    15901 gtttccaggc tcgcaggttc cggcgatttc tccgatcatc gactgaacgc gctgccggat
    15961 ctgatcgctg gtgaa
//
LOCUS       DAOPZO010000032        15239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_4210_length_15239_cov_8.307034, whole genome shotgun sequence.
ACCESSION   DAOPZO010000032 DAOPZO010000000
VERSION     DAOPZO010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 15239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15239
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4210_length_15239_cov_8.307034"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PKI68_03585"
     CDS             <1..797
                     /locus_tag="PKI68_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52806.1"
                     /translation="IDGYGWIARFADTASFAYALGDASMAYRGASEDPMWIGSFKKAG
                     IEQTPANGFGPTPLNLFRRHLVMLRPNIVVLYDELGADRPARFDWLLHSWLEMKLTDD
                     GLSVSRPEGTARLAQLSGTPIKRAVTTGYLVPPDESMMKPNDKINPIWHLTASTEPTK
                     KTYILSVIVLDDPGSTTPLKTAQDSGTIQAGEWTIQAELNGSKPARLLITNKAGDKAL
                     AYAVPQVEVGSKSLHPAYTQSTLIYDQQQGKPGVTELIDTLPRTSY"
     gene            907..2208
                     /locus_tag="PKI68_03590"
     CDS             907..2208
                     /locus_tag="PKI68_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52807.1"
                     /translation="MLHDPSHYEEPAQAEEIILPPRDKEILRRLGGALAEIAVLPVNK
                     ERAELWQKLNDRESVRPMVWINEIPWHEMNVDDELTLQTEHPWARDQEREIRRTLYQW
                     RHLQGDMVVSDFYAVPLAVHSTDFGIRENVDLAKTDESSEIVSRHFNVQIESFDDLEK
                     IRMPVVTHNKEATEFRYQAACDVFGGLMPVKKQGQTHIWFTPWDYLIRWTGIENAMLM
                     MYEEPDFLHAAVERLVDAWMTELDQFVEQNLLSLDCSNTRIGSGGYGYTRELPGNKFD
                     PAAVKPLNMWGCSNAQIFSEVSPEFHWEFAVQHDLRWMKRWGLNYYGCCEPLDGKAGV
                     LEKIPNLRKVSVSPWCKTERAVAELGGKYVLSRKPNPAILAEDVWHPERARKELRDFL
                     DAAKGGKCHVELIMKDISTVRYKPQRLWEWTQIAMEEVRRF"
     gene            2235..2573
                     /locus_tag="PKI68_03595"
     CDS             2235..2573
                     /locus_tag="PKI68_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52808.1"
                     /translation="MTSKERVRSVFEGRIPDRVPLWHGLSAEFTAKALAETGLPDEEA
                     LRVHMGDDFRRIYASYHLPPREVRKVLEIMMPGGGYIAGASHDWVLKETPVKNMLAMV
                     GTVLDCGVYR"
     gene            2632..3492
                     /locus_tag="PKI68_03600"
     CDS             2632..3492
                     /locus_tag="PKI68_03600"
                     /inference="COORDINATES: protein motif:HMM:NF020785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1961 family protein"
                     /protein_id="HNX52809.1"
                     /translation="MKLLMLFIIGAAAAGYCAEPPTPVYKCSFDTPDVLNDWVMEGPG
                     VAEVKDGRLLIYSKWQPELEKIKDRVPLLDPGGDKYFPFIEQWVKEREPEMLPKYMSA
                     ATASGKKSGDFAGGHIQYWNKHSHPANFLIRIKFQAASPWPLHMMTFCARGLHGESIF
                     DPGLAPRYGLAAQYMFGDFSNYRISYWSGTRGTCNMRRAPGRKLTATDSVDLPAYAME
                     RPVQLEIIRWNGRVVFTCDGKKLVDWTDDEPLGDGFFSLRLMAAAKGWYDDYEVYELH
                     QNPFPAGPAN"
     gene            3553..5385
                     /locus_tag="PKI68_03605"
     CDS             3553..5385
                     /locus_tag="PKI68_03605"
                     /inference="COORDINATES: protein motif:HMM:NF020785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1961 family protein"
                     /protein_id="HNX52810.1"
                     /translation="MKRGWIGIVVSFAGLCFGAAPELVYRCTFDTPAALDAWTMEGPG
                     VARIENGQLLIYSKWQPELEAISNQVALIEPGGQNYYPFLESWVKAREPEMLPKYCLD
                     RAVGSTKAGTFMGGHLQFWNKTPHPDNFLIRIKLKALSPYPLHMLTVCARGLQGESVF
                     DRALEPRYGLEYQYLQNDLANYRVSFWSGLRKSCAMRRAPGRVLVAEQPVDLPADVQQ
                     RFVQLELLRWNGRLVFRCDGQTLLEYTDPKPLSGGWFSIRLMAAAKGLYDDYEVYALT
                     EDPFSGTGKGTRIAMRSSPQKDFPTADQVVDGQFIVIGDREPRGSVYRDVLNTCQGLP
                     VYRFEASADANRIEFGTCFASPSVVLSAEEKAELSALGDADAVCDLGHYGDTITYEWN
                     ARFPEPLTKESQGIFAQWHGRPDRTLVTTPSGETKKLAPQEFLNLLKTMRFDNDRGNI
                     GIDLKTGQPNGWRCDGSSGGPIGSFHILDGTMCLMVRNDPAAVSDNTVKVKPKPGRET
                     AQKGNKTGALVFSQPLDKVPINQWMHFKVQIKYSTYRFDSDKALTPGFVKVWIDGNQV
                     ADWKGDIGKNDELGPYFKYGIYKPGPGGFKVDCAGFSQIIEEKH"
     gene            5431..6537
                     /locus_tag="PKI68_03610"
     CDS             5431..6537
                     /locus_tag="PKI68_03610"
                     /inference="COORDINATES: protein motif:HMM:NF025464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparin lyase I family protein"
                     /protein_id="HNX52811.1"
                     /translation="MSLIKTTAMGMMLLSGCLARAGAAETVQVNSADSKALRSADKGE
                     RITVRSSPQKDFPTADQVVDGKFIVVGDREPKAAVYRDVRNTYQGQPVYRFEASEDAN
                     RIEFCTCFASPSVALSSEQKEELSELGEAYAVCDTGRYGDTITYDWYARFPEPFTKES
                     HGIFAQWHGRSDATLVQTPSGEMKKLTPKERLKLLKTMSFKHAEGGIGIDLKTGQPNG
                     WRCDGASGAPIGSLHILEGRMCFQACNDPAALSDSTVRVKAMRPGQETARKGNKTAAM
                     AFSTPLSEVPINQWIHFTVQIKFSSYSQDSDKALTPGFVKVWMDGKQLADWHGDIGKN
                     DELGPYFKYGIYKPGPGGFKVDCAGFSQVIEPKH"
     gene            6583..9648
                     /locus_tag="PKI68_03615"
     CDS             6583..9648
                     /locus_tag="PKI68_03615"
                     /inference="COORDINATES: protein motif:HMM:NF014348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase family 8 super-sandwich
                     domain-containing protein"
                     /protein_id="HNX52812.1"
                     /translation="MNWIKTTTMGMLLLCGGLARAGAAETLLVNYFDIQAGSAAGTEV
                     CGRINLMSNREAHHTPIPADYRLELTADASGLFVLDNQRDSASRLFGALKVAPGRALP
                     AAPGSYKLSVALRAGGVTVAQTNLTVQAVTRTVQDKALAYLDPLARTEVRFYGRRKYT
                     DAQVAAYIEEIEKNRGGFADLGFYKNDVLSYVHTEGKLGEEWEEASNRIGGLGYAYRL
                     SKTYGPGGDAAARLRLKKALYTAIDTFTSRVPVAGEDLLIDGKPISTNFGDGFARLTA
                     EVERSKKMWPTGDPDVLDYHFISHPWRVYDALSGPAIWLLPDLTADIQAGDPVARRVQ
                     EQLVRIFQLAFSNPANYQKVNDPDGRWGVLTDANHTEGAYADANLGHRLRTWMILLGI
                     WNDYNRPITYMPNWYPGYYAKEAGPDFLFMPGWVPQGVLSDLTFWVKHFHRPAHYFVQ
                     SGFHPDGTVSHHLPDSSDIAMHAYGYGWLVEVIDGYTLLRDAPVDLGSHGYQFIADRL
                     LYSYDKMIYNGGMDFAVCGRSYSGSMKKFVEAIPEAIDELLAAKQPGTVITNEAELKA
                     WRDALVQKTQAVSGNYPFWVGQFMVHRRGGGNEKPCYYSVKLKNDRTNGAEDFDKVSK
                     SYHAGSGILQVKVRGDEYEDSRYQWDWHALPGLTEELRTDPLGPKTQAQRGASPFAGM
                     ASDGRYGFAAMEYRAYTNQYASARADKAFFFTETEAVALGCDVARVNAGQGREIMTTV
                     DQTLWVGEITYCFDGKTPVVVPKGEKAELTVQPGQPGWVHQGQVGYVVIPQGAQPVYI
                     RAGTAIPATDPNPKFERVGINSGVVENGKAVSGVSAFHLALGHGIDPQQSGLTRYCYI
                     LAANKTAAEMPAYLADVLKRLVIVANGDGVQGIVDQQLGLTQLAFYKAGGVSTSTGLK
                     VSVDRPALLQLRRDGGQWTFCATDPLHDPAVKELNLKLNQPLAPGVYAYQLGGIYPQP
                     GETVAVEQAGAEVNVKVQLPDMSDDARYHYQAPLYAGMPVQVKIPAKTK"
     gene            9665..12139
                     /locus_tag="PKI68_03620"
     CDS             9665..12139
                     /locus_tag="PKI68_03620"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HNX52813.1"
                     /translation="MMKRSGIWLIGLCMLAASGQAQETRLHAKMRDTFYPGAATVVRA
                     NPVPFLWPSRGHTAEYRFRLGRTPDFSDPSAYTTNVQWACFYPERKLTPGTWYWQIDD
                     QPVQHFEMPATVAFSLELPPFDALLARLPTDHPRLLLRPGEQTAFAARMSPELRQEVL
                     TNADQFLQFIPTVDRLKDAGASATEDQKAQLESIRQLNALQQTYVPVLYLAEAWLLTG
                     DERYGRKSAALAMDFVRSSANAKFSDFAEALAQNLLVLAYDSCYSLLTPADRALMEQR
                     IGAMAENMFRENCGLLEAHVFENHTWQINYISFLKSALALYGHDERAARWLHYASQAW
                     FSRMPATGFVYDGSPWQNGSGYMTASIVTLLQGPLLWERLAGIEAFDHPWFRQAARAI
                     PYYYPPASYSDGFGDGHDSLTHPHWQRGCFMALLAKEFNDPVASWYVQELNRWPNGQK
                     PRTYNPGAKHKLDSDALEWFVTLCDKPMPAPKAPTDQAQALAMPDGGLVSIHSNLAVV
                     SNDVHVAFYSSPFGSGSHALASQNAFNIIAGGRPLFLSSGYYSSFSDPHTLLHYRHTR
                     GHNTILVDGIGQSIGDEGYGQIRRFMTSADLSYAQGDASHAYTGALTDPMWINNMKKA
                     GVEHSRSNGFGNAGLTGYQRHLVFIHPLQTLVIYDDLAADHPAVWNWLLHSPSAMQSL
                     PEEQGFQRFSSDNGLFAGNVWLRGSTPLQSAISTKFFSPAINFLKATDRNGKVRDYPD
                     QWHLSADSKPAAAMRYLAVIQMSPAGAAPLPVVWSNGVARIGSYAIRAELDAQKPAAL
                     EVSCAGTNRFSLASPQIQVMPLFPSR"
     gene            12161..14755
                     /locus_tag="PKI68_03625"
     CDS             12161..14755
                     /locus_tag="PKI68_03625"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HNX52814.1"
                     /translation="MRVSWRWWVVLWLAGTGYGDEALRARYATLQYPPVLLENRPNAA
                     FGPKYNPPSLMWPRPETLPHKAEGRYQVRLSQDPSFPPGATIESGERHWNIYNPHQRL
                     APGAWYWQVKDLKDPQGGWGPVTQFTLTGNEPVYDPPAFADWVGNAKAAHPRLLATPE
                     ELARLRTLYSQKQAQYDGYVANLQLDAWYQAKPALIYNAPETVEALRKKGLSEAEVLR
                     YCNRKTFEQIAAEMRKIDGALRAYVLTGQPRFREMALDRFLQLYALYEDPALRPYTDG
                     DFSLSEIATLSASLCDLLGDAVPAEVHARARERMEAQARKLYREYHGELELEPTSEHA
                     WQTHVRTLLELSLALLGEVPEADEWCEYVYDLWIAKAPVDGLYDGGCAVGISYDMAHY
                     KTLLEVPLRFGAYTGYDFFSIPWYRNVVDYYLYTTLPIHYSCGFGDYHSVRFHNAYYV
                     NFVSLMGLLSNDPRADWFRQQSLAYDDRLKNAPARVESQADYPTFKTALILGTRSLLK
                     WDEGTVWYLVKNGIFEYPSGDTAAVEASLAPAKAFKDTGVAMMHTELAHPERNLMVAF
                     RSSPFGAAYHMHADQNSFNILYGGLPVFYRTGYYTSWADFHTLQSYRHTRAHNTVLPD
                     GMSQSYTYLGFGAIEEFKTGKTISYVRGDASQAYQGQPLTNNAYIEQLQQYGVELSDR
                     NGLGATSLTRFKRHLLLLRPSTVVVFDELEAREPTRWDWKIHALDPLRSTGPGQLAVT
                     TSNATGRLLLCSDQPLTLSITDQFHAEAKDFLNNIGKKGYSIQPQWHATFSAAPSRRA
                     YFLGVMQIEDKTQPPAPLYSMDGAVYAGDWQLEYEPATGVLSGRNRAGQSFRVQPRDN
                     AYAVDVCE"
     gene            14820..>15239
                     /locus_tag="PKI68_03630"
     CDS             14820..>15239
                     /locus_tag="PKI68_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52815.1"
                     /translation="MLNRQWTKWICGLMLVAAGAAASAETSSNPIWGRMGRAPVNGGF
                     RQDDYWVWGSSIIKGDDGKYHMYAERWPKKVAFHPGWMVASDIAHAVSDTMVGPYTFS
                     DVAIGRRSPEYWDGCSQYNPKIFKHGDTYILFYEGSTH"
BASE COUNT         3010 a         4366 c         4943 g         2920 t
ORIGIN      
        1 ccatcgacgg ctacggctgg atcgcgcgct tcgccgacac cgcctccttc gcctacgcgt
       61 tgggcgacgc ttcgatggcc tatcgcggag cgtccgaaga ccccatgtgg atcggcagtt
      121 tcaaaaaagc cggcatcgaa cagacgcctg ccaacggttt cggcccgacg ccgctcaatc
      181 tcttccgccg gcacctggtg atgcttcgtc ccaatatcgt tgtgctttac gacgagctgg
      241 gcgcagaccg tccggcccgc ttcgactggc tgctgcacag ctggctggaa atgaagctga
      301 ccgacgacgg cctcagcgtc agtcgtccgg agggcaccgc ccgcctcgcg cagctttccg
      361 gaacaccgat caagcgcgcc gtcaccaccg ggtacctcgt gccgccggat gaatccatga
      421 tgaaacccaa cgataagatt aatccgattt ggcatttaac cgcctccacc gaaccgacca
      481 agaagaccta tatcctctcc gtcatcgtgc tcgacgatcc gggttcgacc accccgctga
      541 agaccgcgca ggattccggc accatccagg ccggcgaatg gacgatccag gccgagctca
      601 acggctcgaa gccggcccgt ctgctgatta cgaacaaagc gggtgacaaa gcgctggcct
      661 atgccgtgcc gcaggttgag gtgggctcga aatcgctgca ccccgcctac acccagtcga
      721 cgctgattta cgatcagcag cagggcaagc ccggcgtgac ggaactgatt gatacgctgc
      781 cccgcacgtc atattagtct gagctgaggt ttgacgccgg gcctgctgtc aggcgcaact
      841 atcggcagaa tcagaggcga cgcagaaggc agcagatctt ttaaaaaaga taattcagga
      901 gagtttatgt tgcacgatcc gagtcattat gaagagccgg ctcaggcgga agaaatcatt
      961 ctgccgccgc gcgataaaga aattttgcgg cggcttggcg gcgcgctggc ggagatcgcc
     1021 gtactgccgg ttaataaaga gcgggcggag ctttggcaga agctcaatga ccgggaatcg
     1081 gtgcggccca tggtctggat caatgaaatc ccgtggcatg agatgaatgt tgatgatgag
     1141 ctgactctgc agacggagca tccgtgggcc cgcgatcagg agcgcgaaat ccgccgcaca
     1201 ttgtaccaat ggagacattt gcagggagat atggtcgtca gtgattttta cgccgtgccg
     1261 ctggcggttc acagtacgga tttcggaatc cgtgaaaatg ttgatctggc caaaacagat
     1321 gagtcgagcg aaattgtttc tcgtcatttc aatgtgcaga tcgagtcgtt cgatgacctt
     1381 gaaaagatca ggatgcctgt ggttacgcat aataaggagg caactgaatt ccgttaccag
     1441 gcggcgtgcg atgtgttcgg cggtttgatg ccggttaaaa aacaagggca gacccatatc
     1501 tggtttacgc cctgggatta tttgattcga tggaccggga tcgagaacgc aatgctgatg
     1561 atgtatgagg agccggactt cctgcatgcg gcggttgagc ggctggtgga cgcatggatg
     1621 acagaactcg atcagttcgt tgaacagaat cttctttcac tggattgcag caacacgcgt
     1681 atcggatcgg gaggctacgg ctatacccgc gagctgccgg gaaataagtt cgatccggcg
     1741 gcggtaaagc ctctcaacat gtggggctgt tccaatgcac agatcttttc tgaagtgtct
     1801 ccggaattcc attgggagtt tgccgtacag catgatctgc gctggatgaa gcgctgggga
     1861 ctgaactatt acggctgctg cgaaccgctc gacggcaaag ccggcgtact ggagaagatt
     1921 cccaatctgc gcaaggtgtc ggtcagcccc tggtgtaaaa ccgaacgcgc tgttgcagag
     1981 ctgggcggta aatatgtcct gagccgcaag cccaatccgg cgattcttgc cgaggatgtc
     2041 tggcatcccg aacgggcccg gaaggaactc cgcgattttc ttgatgccgc gaaaggtggc
     2101 aaatgccacg tggagttgat catgaaagat atttccacag tgcgctacaa acctcaaagg
     2161 ctgtgggaat ggacgcaaat tgccatggaa gaagtccgcc ggttctgaat gtaaacgaaa
     2221 gcggagatac agggatgacc tcgaaggagc gtgtacgatc agttttcgaa ggacgtatac
     2281 cggaccgggt tcccttgtgg cacgggttgt ctgccgagtt cactgccaag gctctcgctg
     2341 aaaccggctt gccggacgaa gaggcgttaa gagttcacat gggcgacgat ttccggcgca
     2401 tatatgcttc gtatcatctt ccgccacggg aggtcagaaa ggttctcgaa attatgatgc
     2461 ccggcggcgg atatattgcc ggtgccagcc atgactgggt gctgaaagaa actccggtga
     2521 aaaacatgct ggcaatggtc ggcacggtgc tggactgtgg tgtgtaccgc tgacagcagc
     2581 agacgatcag gtgcagccgg tgcaaaaaac cgtcatatcg acaggggggt tatgaaatta
     2641 ctgatgttat ttattatcgg agcggcggcg gccgggtact gtgcggagcc tccgacgccg
     2701 gtttacaaat gttcatttga tacaccggat gttttgaatg actgggtgat ggaggggccg
     2761 ggcgtcgctg aagtgaagga cggacgtctg ctgatctatt ccaaatggca gccggagctg
     2821 gaaaagatca aagatcgtgt tccgcttctt gatccgggag gggataaata ctttccgttc
     2881 attgagcagt gggtgaagga acgcgaaccg gagatgcttc caaaatatat gtcggcggca
     2941 accgcttccg gaaagaaatc cggtgatttc gccggcgggc acattcagta ttggaataag
     3001 cattcgcatc cggcgaattt tttaatacgc atcaagtttc aggcggccag tccatggcct
     3061 ttgcacatga tgacattctg cgcccgcggt ctccacggcg agagcatttt cgatcccggg
     3121 ctggcgcccc gctatggtct ggcggcgcag tatatgttcg gcgatttctc caattatcgc
     3181 atcagttact ggagcggaac gcggggcaca tgcaacatgc gccgcgcgcc gggtcgcaaa
     3241 ctgacggcga ctgattcggt cgatctgccg gcctatgcga tggaacgccc cgtgcagctg
     3301 gaaattatcc ggtggaacgg gcgggtggtc tttacgtgcg acggaaaaaa actggtggac
     3361 tggacggatg atgaaccgct tggcgacggg ttcttttctc tgcggctgat ggctgccgca
     3421 aagggctggt atgatgatta tgaagtgtac gagcttcatc aaaacccgtt tcccgctggc
     3481 ccggcgaact gattaaatgc tgaaccgtca gacggaagcc gataacgtcc ggttttttgc
     3541 aaggaggagt gaatgaaaag gggatggatt gggattgttg tgagctttgc cgggctgtgt
     3601 ttcggcgcgg cgccggagct ggtgtaccgc tgtacgttcg acacgccggc cgcgctcgat
     3661 gcgtggacga tggaaggtcc gggggtcgcc cggattgaaa acggccagct gctgatttat
     3721 tccaagtggc agccggagct tgaagcgatc agcaaccagg tggcgctgat cgagcccggc
     3781 gggcagaatt attatccctt tctcgagtcg tgggtgaagg cgcgcgagcc ggagatgctg
     3841 ccgaagtatt gcctggaccg ggcggtcggc agtacgaagg ccggcacgtt tatgggcggg
     3901 caccttcagt tctggaacaa aacgccgcat ccggacaact ttctgatccg catcaagctc
     3961 aaggcgctga gtccctatcc gctgcatatg ctgacggtct gcgcccgcgg cctgcagggc
     4021 gagagcgtgt tcgaccgcgc tctggagccg cgctacgggc tggaatacca gtatctgcaa
     4081 aacgatctgg cgaactaccg cgtgagcttc tggtccggcc tgcgcaagag ctgcgccatg
     4141 cgccgggcgc cgggccgcgt gctggtggcg gagcagccgg tggatctgcc ggcggacgtg
     4201 cagcagcgct ttgtgcagct cgaactgctg cgctggaacg gccggctggt gttccgctgc
     4261 gacggtcaga cgctgctgga gtataccgac ccgaagccgc tgtccggcgg ctggttttcg
     4321 atccgcctga tggcggcggc gaaggggctg tatgacgatt atgaagtgta tgcgctgacg
     4381 gaagatccgt tcagcggcac gggcaagggg acgaggattg ccatgcggtc gtcgccgcaa
     4441 aaagattttc cgacagcgga tcaggtggtg gacggtcagt ttattgtcat cggcgatcgc
     4501 gagccgaggg ggtcggtgta ccgcgatgtg ctgaatacct gccagggact gccggtctac
     4561 cggttcgaag cgtcggcgga cgccaaccgc attgagttcg gcacctgctt cgcatcgccg
     4621 tccgtggtgc tttcggcgga agagaaggcg gagctgtcgg cgctgggcga tgcggatgcc
     4681 gtgtgcgatc tgggccatta cggcgatacg atcacttatg aatggaatgc gcgttttccg
     4741 gagccgctca cgaaggagtc gcaggggatt tttgcgcagt ggcacggccg gccggaccgt
     4801 acgctggtga cgacgccgtc cggcgagacg aagaagctgg cgccgcagga gtttctgaat
     4861 ctgctgaaaa cgatgcgctt tgacaacgat cggggcaata tcggcatcga tctcaaaacc
     4921 ggtcagccga acgggtggcg ctgcgacggc tcctcgggcg ggccgatcgg ttcgttccat
     4981 attctggatg ggacgatgtg tctgatggtg cgcaacgatc cggcggcggt gtcggataat
     5041 acggtgaagg tgaagccgaa gccggggcgc gaaacggcgc aaaagggcaa taaaaccggg
     5101 gcgcttgttt tctcgcagcc gctggacaag gtgccgatta accagtggat gcatttcaag
     5161 gtgcagatca aatattccac ctaccgcttc gattccgaca aggcgctgac gccgggcttt
     5221 gtgaaggtct ggatcgacgg caatcaggtg gccgactgga aaggcgatat cggcaagaac
     5281 gatgagctgg ggccgtactt caaatacggc atctacaaac cgggtccggg cggcttcaaa
     5341 gtagattgcg ccgggttctc acaaatcatc gaagaaaaac actaaaccgc cggaacggcg
     5401 cgttctgcgt tccgcaacga ggagacttct atgagtttga tcaagacaac ggcgatggga
     5461 atgatgctgc tgagcggatg tctggcgcgg gccggggccg ccgaaaccgt gcaggtgaat
     5521 tctgcggaca gcaaagcgct ccgcagcgca gacaagggcg agcggattac cgtgcggtcg
     5581 tcaccgcaaa aagattttcc gacagcggat caggtggtgg acgggaagtt catcgtcgtc
     5641 ggagatcgcg agccgaaggc ggcggtgtac cgcgatgtgc ggaataccta tcaggggcag
     5701 ccggtctacc gcttcgaagc gtcggaggac gccaaccgca ttgagttctg cacctgcttc
     5761 gcctcgccgt ccgtggcgct ttcgtcggag cagaaggagg agctgtcgga gctgggcgag
     5821 gcctatgccg tctgcgatac gggccgttac ggcgacacga tcacctatga ctggtatgcg
     5881 cgttttccgg agccgttcac gaaggagtcg catggaatat ttgcgcagtg gcacggccgg
     5941 tcggacgcta cgctggtgca gacgccgtcg ggcgagatga aaaagctgac gccgaaggag
     6001 cgtctgaagc tgctgaaaac gatgagcttt aaacacgccg agggcggtat cggcatcgat
     6061 ctcaaaaccg gtcagccgaa cgggtggcgc tgcgacggcg catccggcgc gccgatcggt
     6121 tcgttacata ttctggaagg ccggatgtgt tttcaggcct gtaatgatcc ggcggctctg
     6181 tcggacagca ccgttcgggt caaggcgatg cggccgggcc aggaaacagc gcgcaagggc
     6241 aataaaaccg cagccatggc cttctccacg ccgctgtccg aggtgccgat caaccagtgg
     6301 attcatttta cggtgcagat caagttttcc tcctacagtc aggattccga taaggcgctg
     6361 acgcccggct ttgtgaaggt ctggatggat ggcaagcagc tggcggactg gcatggcgac
     6421 atcggcaaga acgatgagct gggaccgtac ttcaaatacg gcatctacaa gccggggccg
     6481 ggcggcttca aagtggattg cgccggattc tcgcaagtga tcgaacccaa acactagacc
     6541 ggcgggccgg cgcgtgctgc gtgccgcaac ggggagactt ctatgaattg gatcaaaaca
     6601 acgacgatgg gaatgctgct gctgtgcgga ggcctggcgc gggccggggc cgccgaaacc
     6661 ctgctggtga attatttcga catccaggcg ggcagcgccg cgggcacgga ggtgtgcggc
     6721 cggatcaatc tgatgtcgaa cagggaggcg caccacacgc cgatcccggc cgattaccgg
     6781 ctcgaactca cggccgacgc gtcgggtctg ttcgtgctcg ataaccagcg cgattccgcg
     6841 agccggctgt tcggcgcgct gaaagtggcg ccgggccgcg cgcttccggc ggcgccgggc
     6901 agctacaaac tgtcggtggc gctgcgcgcc ggcggcgtga cggtggcgca aacaaacctg
     6961 acggttcagg cggtgacccg cacggttcag gataaagcgc tggcctatct cgacccgctg
     7021 gcccgcaccg aggtccgttt ctacggccgc cggaaatata ccgacgcgca ggtggcggcc
     7081 tatatcgaag agatcgaaaa gaaccgcggc ggctttgccg acctcggctt ttacaagaac
     7141 gacgtgctga gctatgtgca caccgagggc aagctgggcg aggagtggga agaagcctcc
     7201 aaccgcatcg gcggactcgg ctatgcgtac cgcctctcga aaacctacgg accgggcggc
     7261 gacgcggcgg cccgcctgcg cctcaaaaaa gcgttgtaca cggcgatcga cacctttacg
     7321 tcgcgggttc cggtggccgg cgaggatttg ctcatcgatg gaaagccgat ttcaacgaac
     7381 ttcggcgacg ggtttgcgcg gctgaccgca gaggtcgagc gctcgaagaa aatgtggccg
     7441 accggcgatc cggatgtcct cgattatcat ttcatctcgc atccctggcg ggtctacgac
     7501 gcgctgtccg gcccggccat ctggctgctg cccgatctga cggccgacat tcaggccggc
     7561 gatccggtgg cgcggcgggt gcaggaacag ctcgtccgca ttttccagct ggcgttcagc
     7621 aatccggcca actaccagaa ggtgaatgat ccggacggcc gctggggcgt tttgaccgac
     7681 gcgaatcata cggaaggggc ctacgccgac gccaacctgg gccaccgcct gcgcacctgg
     7741 atgattctgc tgggtatctg gaacgactac aaccgcccga tcacctacat gcccaactgg
     7801 tatcccggtt attatgccaa agaagccggg ccggatttcc tctttatgcc gggctgggtc
     7861 ccgcagggcg ttctgtcgga cctgaccttc tgggtgaagc acttccaccg cccggcgcat
     7921 tactttgtac agtccggatt tcatccggac ggcacggtgt cgcaccatct gccggactct
     7981 tcggatatcg ccatgcacgc ctacggctac ggctggctgg tcgaagtgat cgacggctac
     8041 acgctgttgc gcgatgcgcc ggtcgacctc ggcagtcacg gctatcagtt catcgccgac
     8101 cggctgctct acagctacga caagatgatc tacaacggcg gcatggactt cgccgtctgc
     8161 ggccgcagct attccggcag catgaagaag tttgtggaag cgattccgga agcgattgat
     8221 gaactgctgg ccgccaagca gcccggaacc gtcattacga acgaagccga gctgaaagcc
     8281 tggcgcgacg cgctcgtgca gaaaacacag gccgtctccg gcaactatcc cttctgggtc
     8341 ggccagttca tggtgcatcg ccgcggcggc ggaaacgaaa agccctgtta ttactcggtg
     8401 aaactgaaaa acgaccggac gaacggggcc gaggatttcg acaaggtgtc caagtcgtac
     8461 cacgccggca gcggcatcct gcaggtgaag gtccgcggcg atgaatacga agacagccgc
     8521 tatcaatggg actggcacgc gctgcccggc ctgaccgagg aactgcgcac cgatccgctg
     8581 ggcccgaaaa cgcaggccca gcgcggcgcc agtccgttcg ccggcatggc ttccgacggc
     8641 cgctacggtt tcgccgccat ggaataccgc gcctatacca accagtatgc ctcggcgcgc
     8701 gccgataaag ccttcttctt caccgaaacc gaagccgttg ccctgggctg cgatgtggcc
     8761 cgggtcaatg ccgggcaggg ccgcgaaatc atgaccacgg ttgaccagac cctttgggtc
     8821 ggagagatca cctactgctt cgacgggaaa acgccggtgg tggttcccaa aggcgaaaag
     8881 gccgagctca ccgtgcagcc cggccagccc ggctgggtgc atcagggcca ggtcggctac
     8941 gtcgtgattc cgcagggtgc ccagccggtt tatatccgcg ccggcaccgc gatccctgcg
     9001 accgacccga atccgaaatt tgagcgggtc ggcatcaaca gcggcgtcgt cgagaacggc
     9061 aaggccgtga gcggcgtatc ggcgtttcat ctggcgctcg gacacgggat cgatccgcag
     9121 cagagcggct tgacgcgcta ctgctatatt ctggccgcca acaaaacggc ggcggaaatg
     9181 ccggcctatc tggcggacgt tctgaagcgg ctggtgatcg tcgccaacgg cgacggcgtg
     9241 caggggattg tcgatcagca gctgggcctg acgcagctgg cgttttataa agccggcggg
     9301 gtctcgacct cgaccgggct gaaggtttcg gtcgatcgtc ccgcgctgct gcagctgcgc
     9361 cgcgacggcg gacaatggac tttctgcgcg accgatccgc tgcacgatcc ggccgtgaag
     9421 gagctgaacc tgaaactcaa tcagccgctg gctccggggg tttatgccta tcagctcggc
     9481 ggcatctatc cgcagcccgg cgaaacggtt gcggtggagc aggctggcgc agaagtgaac
     9541 gtcaaggtgc agctgccgga catgagcgat gatgcgcgct atcactatca ggcgccgctc
     9601 tatgccggaa tgccggtgca ggtgaagatc ccggcgaaaa cgaagtaaaa gcaggagaga
     9661 gcaaatgatg aagcggagcg gtatctggtt gattggactg tgcatgttgg cggcctccgg
     9721 gcaggcgcag gagacgcggt tgcatgcgaa aatgcgcgac accttttatc ccggcgcggc
     9781 caccgtcgtg cgggctaatc ccgtgccgtt tctctggccg tcgcgcggcc atacggcgga
     9841 ataccgtttc cggctgggcc gcacgccgga cttttccgat ccctccgcgt ataccaccaa
     9901 cgtgcagtgg gcctgcttct atccggaacg caagctcacg cccggcacct ggtactggca
     9961 gatcgacgac cagccggtac agcattttga aatgcctgcg accgtggcct tttcgctgga
    10021 gctgccgccg ttcgacgcct tgctggcgcg cctgccgacc gatcatccgc gcctgctgct
    10081 gcgtcccggc gagcagaccg cttttgccgc gcggatgagc ccggagctgc ggcaggaagt
    10141 tctgacgaac gccgatcagt ttttgcagtt tattccgacg gtggaccgcc tgaaggatgc
    10201 cggagcgtcc gcgacggagg atcagaaggc gcagctcgaa tcgatccggc agctgaatgc
    10261 gctgcagcag acctatgtgc cggtgcttta tctcgccgag gcgtggctgc tcaccggcga
    10321 tgagcgctac ggccgcaaga gcgcggcgct ggcgatggat tttgtccgca gctccgccaa
    10381 cgcgaagttc tcggactttg ccgaagcgct ggcgcagaac cttctcgtgc tggcgtatga
    10441 cagctgctac agcctgctga cgcccgccga ccgggcgctg atggaacagc gcatcggcgc
    10501 gatggcggaa aacatgttcc gcgaaaattg cggactgctc gaagcgcacg tgttcgaaaa
    10561 tcacacctgg cagatcaact acatcagctt cctgaagagc gcgctggctt tatacggcca
    10621 cgacgaacgg gccgcgcggt ggctgcacta tgccagtcag gcgtggttct cgcggatgcc
    10681 ggcgaccggc tttgtctacg acggcagccc gtggcagaac ggatcgggtt atatgacggc
    10741 cagtatcgtc accctgttgc agggaccgct gctctgggag cggctggcgg gcatcgaagc
    10801 gtttgatcat ccgtggttcc gccaggcggc ccgcgcgatt ccgtattact atccgccggc
    10861 ttcctactcc gacggcttcg gcgacggcca cgacagcctg acgcatccgc actggcagcg
    10921 cggctgtttc atggcgctgc tcgcgaagga gtttaatgat ccggtggcgt cgtggtatgt
    10981 ccaggagctg aaccgctggc cgaacggcca gaagccgcgc acctacaatc ccggcgccaa
    11041 gcacaagctc gacagcgatg cgctcgagtg gttcgtgacg ctctgcgaca agccgatgcc
    11101 ggcgccgaaa gccccgaccg atcaggcgca ggctctcgcc atgccggacg gcggactggt
    11161 ttcgatccat tcgaatctcg cggtggtttc gaacgatgtt cacgtcgcct tctactccag
    11221 cccgttcggc tccggcagtc acgcgctggc gagccagaac gccttcaata tcatcgccgg
    11281 cggccgtccg ctgtttctca gcagcggtta ttacagctcc ttctcggacc cgcacacgct
    11341 gctgcactac cgccacaccc gcggccacaa caccattctg gtggacggca tcggccagtc
    11401 gatcggcgat gaaggctacg ggcagatccg ccggtttatg acgtcggcgg atctcagcta
    11461 tgcgcagggc gacgcgtcgc atgcgtatac cggcgcgctg acggacccga tgtggatcaa
    11521 caatatgaag aaggcgggcg tggagcattc gcgcagcaac ggcttcggga atgcgggcct
    11581 caccggctat cagcgccacc tggttttcat ccatccgctg caaacgctgg tgatctacga
    11641 cgatctcgcg gccgatcatc cggcggtctg gaactggctg ctgcacagcc cgtccgccat
    11701 gcagtcgctg ccggaagagc aggggttcca gcgcttcagc agcgacaacg ggctgttcgc
    11761 cgggaacgtc tggctgcgcg gcagtacgcc gctgcagtcg gccatttcca cgaaattttt
    11821 ctcgccggcc atcaacttcc tgaaggccac cgatcgcaac ggcaaggtgc gcgactatcc
    11881 cgaccagtgg cacctttcgg cggattccaa gccggcggcg gccatgcgtt atctggcggt
    11941 gatccagatg tcgcccgcgg gcgccgcgcc gctgccggtg gtctggagca acggtgtggc
    12001 gcgcatcggt tcgtatgcga tccgggccga gctcgacgcg cagaagccgg cggctctgga
    12061 agtgtcctgc gcggggacga accggttttc gctggcgtcg ccgcagattc aggtcatgcc
    12121 tctgtttcct tcccgttaat caaaatgtaa ggagttcatg atgcgcgttt cgtggcggtg
    12181 gtgggttgtg ctgtggctgg cgggaaccgg gtatggcgat gaggcgctgc gtgcgcgcta
    12241 tgcgacgttg cagtatccgc cggttctgct ggagaaccgc cccaacgccg ccttcggccc
    12301 gaaatataat ccgccgagtc tgatgtggcc gaggccggaa acactgccgc ataaggcgga
    12361 aggccgctat caggtgcggc tttcgcagga tccgtcgttc ccgcccggcg cgaccatcga
    12421 gtccggcgag cggcactgga atatctataa tccgcatcag cggctggctc cgggcgcgtg
    12481 gtactggcag gtcaaagatt tgaaggatcc gcagggcggc tgggggccgg tgacgcagtt
    12541 tacgctgacc ggcaacgagc cggtctatga tccgccggcg tttgccgact gggtcggcaa
    12601 cgccaaggcc gcgcacccgc gcctgctggc gacgccggag gagctggcgc ggctgcggac
    12661 gctttattcg cagaagcagg cgcagtacga cggctatgtg gcgaaccttc agctggacgc
    12721 ctggtatcag gccaagccgg cgctgattta taacgcgccg gagacggtgg aggcgttgcg
    12781 gaagaaagga ctttcggagg cggaggtgct gcgctattgc aaccggaaaa cgttcgagca
    12841 gatcgccgcc gagatgcgca agatcgacgg cgcgctgcgg gcctatgtgc tgaccgggca
    12901 gccgcgcttc cgggaaatgg cgctggatcg cttcctgcag ctctacgcgc tgtacgaaga
    12961 tccggcgctg cgtccgtata ccgacggcga tttttcgttg agcgagattg cgacgctgtc
    13021 ggcttcgctc tgcgatctgc tgggcgatgc ggtgccggcg gaggtgcatg cccgggcccg
    13081 cgagcgcatg gaggcccagg cccgcaagct gtaccgggaa tatcacggcg agctggagct
    13141 ggagccgacg tcggaacatg cgtggcagac ccatgtgcgc acgctgctgg agctgtcgct
    13201 ggcgctgctc ggcgaggtgc cggaggccga cgagtggtgc gagtatgtgt acgatctctg
    13261 gatcgccaag gcgccggttg acggtctgta tgacggcggc tgcgcggtcg gtatttccta
    13321 cgacatggcg cattacaaga cgctgctgga agtgccgctg cgcttcgggg cctataccgg
    13381 ctatgatttc ttttcgattc cctggtaccg gaacgtcgtc gattactatc tctacaccac
    13441 gctgccgatc cactattcct gcggcttcgg cgattaccac agcgtgcggt ttcacaacgc
    13501 ctattacgtc aacttcgtca gtctgatggg gctgctcagc aacgacccgc gcgcggactg
    13561 gttccgccag cagagcctgg cctatgacga ccggctgaaa aacgccccgg cgcgcgtcga
    13621 gtcgcaggcc gactatccga cgtttaagac ggcgttgatt ctggggacgc gctcgctgct
    13681 gaagtgggat gaggggacgg tctggtatct ggtgaaaaac gggatttttg aatatccgtc
    13741 gggagatacc gcggcggtcg aagcttcgct ggcaccggcc aaggcgttca aggataccgg
    13801 cgtggcgatg atgcatacgg agctggcgca tccggagcgc aatctgatgg tcgcgttccg
    13861 ctccagcccg tttggcgcgg cgtaccacat gcacgccgac cagaactcgt tcaacattct
    13921 ctacggcggg ctgccggtgt tttatcgcac cggctattac accagctggg cggatttcca
    13981 caccttgcag tcctaccgcc acacccgcgc gcataacacg gtgctgcccg acgggatgag
    14041 ccagagctat acctatctgg gtttcggggc cattgaggag ttcaaaaccg ggaaaaccat
    14101 ttcctatgtg cgcggcgacg cgtcgcaggc ctatcagggc cagccgctca cgaacaacgc
    14161 ctacattgaa cagctgcaac agtacggcgt ggagctgagc gaccgcaacg gtctcggcgc
    14221 cacttcgctc acccgcttta agcgccatct gctcctgctg cggccttcaa ccgtggtggt
    14281 gttcgacgag ctcgaagccc gcgagccgac gcggtgggac tggaagatcc acgcgctgga
    14341 tccgctgcgg tccaccggtc ccgggcaact ggcggttacg acgtcgaacg cgaccgggcg
    14401 gctgttgttg tgcagcgacc agccgctgac gctgtcgatc accgatcagt ttcacgccga
    14461 ggcgaaggat tttctgaaca acatcggcaa gaagggctat tcgattcagc cgcagtggca
    14521 cgcgacgttt tcggccgcgc ccagtcgccg ggcctacttt ctgggcgtga tgcaaatcga
    14581 ggataaaacg cagccgcctg cgccgctcta ctccatggac ggcgcggtgt atgcgggcga
    14641 ctggcagctg gagtatgagc cggcgaccgg cgtgctgagc gggcgcaacc gcgccggaca
    14701 atcgttccgc gtgcagccgc gcgacaacgc ctatgcggtt gatgtgtgcg agtaaaccga
    14761 tgcgggaagt ccggcgcggc ggtgggtgta aaggggtttg aggagaaagg aattcggaga
    14821 tgctgaatcg acagtggacg aaatggattt gcggattgat gctggttgcg gcgggggctg
    14881 cggcgtcggc ggaaacttcc agcaacccca tctggggacg catggggaga gccccggtga
    14941 atggcgggtt ccggcaggat gattactggg tgtggggcag ctcgattatc aagggcgatg
    15001 acggaaagta tcacatgtac gccgaacgct ggccgaagaa ggtcgctttc catccgggct
    15061 ggatggtggc ttccgatatt gcgcatgcgg tgtccgatac gatggtcggc ccgtacacgt
    15121 tcagcgatgt ggccatcggc cggcgcagtc cggaatactg ggacggctgt tcgcagtata
    15181 acccgaaaat cttcaagcat ggcgacacct acattctctt ctatgaaggg tccacccat
//
LOCUS       DAOPZO010000033        14811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_4396_length_14811_cov_10.731973, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000033 DAOPZO010000000
VERSION     DAOPZO010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 14811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14811
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4396_length_14811_cov_10.731973"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2598
                     /locus_tag="PKI68_03635"
     CDS             <1..2598
                     /locus_tag="PKI68_03635"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNX52816.1"
                     /translation="HLGKDLLANTLIQTIQVFSKEEWLAAEPDMTAPIFDDHQEVKVN
                     VIELPDDDAALMKISSDGILSLSLEEMRAIRNHYLSDAVKAHRAELGMPEWPTDVELE
                     CIAQTWSEHCSHKIFAGTVEYKDAETGKEEIINSLYKTYVKASTKKVAEKIDWLVSVF
                     TDNAGVIKFNEDLHLVYKVETHNSPSALDPFGGSMTGIVGVNRDPMGTGMGAQLVSNV
                     WGYCLGSPFYDKEIPAGLMHPRRIRDGVHAGVIAGGNESGIPWSRGFECFDERYLGKP
                     LVYCGTMGTLPVSVGGRPSERKEVKVGDWTVMCGGRIGKDGIHGATFSSEELRTESPA
                     QAVQIGDPLTQRKLYEFLMEARDLGLFRCITDNGAGGISCSFGEMGKYSGGCECELKD
                     APLKYEGLQPWEILVSEAQERMTLAVQPECREAFEKLAKQRDVEAAFMGKYNDSGYFT
                     VKYDGKVVASLDMDFLYETGCPKLHMKGVWKKPEIPAPEKAKGGNRTKQLRQMMGRLN
                     ICSREYKSRIYDGEVKGLSVVKPFVGVNADVPSDATVMRIEYNKDRGAILAEGINPWF
                     SDIDTYHMTASVIDESIRRVISVGGRLDRIAILDNFCWPDPVQSSKTPDGEYKMAQLV
                     RSNKALYDYTVAFGTPAVSGKDSCKNDSTRGGKKISIPPTLLISSIGQIDDVKKAVTM
                     PLKEAGSLIYMVGVTKNELGASEWNRMLAEEQGNPWNYGGDVPTVDAKAALALYRAMN
                     QAQAEGILLSSHTPSKGGLAVGFALICMGGQLGAEIDLAKVPTPGKRKLKDDQILFSE
                     SNSRFIVSVKPENAARFEALFAGLPAAQIGTVTAEKKLTVKGVKGATRIDTDLETLRK
                     PFKKTLYGI"
     gene            2740..3048
                     /locus_tag="PKI68_03640"
     CDS             2740..3048
                     /locus_tag="PKI68_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52817.1"
                     /translation="MVFDLKQKESLKKDLVSCLSGDQEIQRIVIFGSFVGSSSPEDMD
                     VAVFQNSAEPYLSLAVKYRRQTRSISRRIPLDIIPLRAGVTDDPFLKEVERGETVYER
                     "
     gene            3038..3424
                     /locus_tag="PKI68_03645"
     CDS             3038..3424
                     /locus_tag="PKI68_03645"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNX52818.1"
                     /translation="MSVERSRWIAYADENLAIARLALDSGYYNACLQNVQQAGEKYLK
                     ALLLACGLEFRKTHSIETLRNQLAGEGLDAALSDEDCILLDSIYMPSKYPFGSVLPDF
                     NPDADICDQCIRIADAVRKAVFSAGV"
     gene            3424..5178
                     /gene="pabB"
                     /locus_tag="PKI68_03650"
     CDS             3424..5178
                     /gene="pabB"
                     /locus_tag="PKI68_03650"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HNX52819.1"
                     /translation="MFEVLLQHGNGWLRFPEPVEVLTARTPEEVFRCLERIETSGLWA
                     AGFISYEAAGAFDSALKTHAPGSLPLLQFGLFENVEQTFLSAQMHRQECLRHYQLGEW
                     KPSVTRGEYSEAIAKIKERIAAGDTYQVNYTFRLNADFSGSPFDFFCDLAAAQQGRYA
                     AFIETPEFAVCSASPELFFELKDGVITSRPMKGTLPRGLTAEADRAAAEALKNSAKDC
                     AENIMIVDMIRNDIGRIAEAGSVETVSRFDVEKYPTVWQLTSTVQGKVCGAGFQSAHE
                     QAGMPAPQIFKALFPCASITGAPKAKTMEIIRGLEKTPRGIYTGAIGFIAPDGAAQFS
                     VAIRTAVIQAGRAEYGTGGGIVWDSDADSEYDEALSKARILTRRFPEFQLLETMLWED
                     GEIFLLERHLKRLAGSAEYFGFNLDESKVRDRLRDLKSDEPMRVRLLLDKDGRFEVQA
                     FKMKRGRDAASTLAIAREPVDSGDVFLYHKTTNRAVYERARADFPEADDVLLYNERGE
                     ITESCIANVVVELDGRKVTPPVSCGLLAGTFRNELLERGEIEEQVVSVEELRRADAIW
                     LINSVRKWRKVSLNCSAS"
     gene            5175..5972
                     /locus_tag="PKI68_03655"
     CDS             5175..5972
                     /locus_tag="PKI68_03655"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNX52820.1"
                     /translation="MMKPKILIITGYGVNCEAESSRAWELAGAEPVKVHLNDLLDHPA
                     QMRDFRGLMFIGGFSYGDHMTSGHVFALRVKHHLREELQKFIDDGKIILGVCNGFQTM
                     TKIGLLPGLNGEYFEPKVSLMQNDCGHFQNFWVNLKFEANSPCIFTKGIAGMPMPVRH
                     GEGKIFTTDKTVIEQIEKLGCAAARYADADGNPTMDFPANPNGSLNAIAGLCDPTGRI
                     FGLMPHPEAYLFPENHPNWDVQKLNGTLPAQGLGLKLFENGVKFLAR"
     gene            6107..7207
                     /gene="ilvC"
                     /locus_tag="PKI68_03660"
     CDS             6107..7207
                     /gene="ilvC"
                     /locus_tag="PKI68_03660"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNX52821.1"
                     /translation="MAVIDFGGTKETVVTRKEFPLAKARKVLKNEIIAVIGYGVQGPA
                     QALNLRDNGFNVIVGQSKKFMQDWNRAVKDGFVPGKTLFEIEEAVQKGTVIQYLLSDA
                     AQKLCWPQIKKNLKPGDALYFSHGFSIVYKDLTKVIPPKNVDVILVAPKGSGLNVRRN
                     FLTGAGINSSFGVFQDATGRAEERCMALGIGIGSGYLFPTTFEKEVTSDLVGERGILM
                     GALAGVMEAQYSVLRKNGHSPSEAFNETVEELTQSLIRLVDENGMDWMFSNCSATAQR
                     GALDWSPKFKKATLPVFNDLYKAVKTKSEAKRVLKVCGAKDYKERLTKELKAIHDSEM
                     WQAGAASRSLRPKGEAKVIAKGTKGVGGRAAN"
     gene            7370..10912
                     /locus_tag="PKI68_03665"
     CDS             7370..10912
                     /locus_tag="PKI68_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase family 8 super-sandwich
                     domain-containing protein"
                     /protein_id="HNX52822.1"
                     /translation="MKHSLIMIGSGLLLGFAAAQAAPLITLNGGALVTTEAGKPFVDP
                     GATVVNPEAPAFEPVVLKTNGTVKIYTPGDYSLTYTAVNAAGVSNTAVRTVRVVISRE
                     LLANYFDIETNAPAGLEVIGKVHLLANYNAHRYPIPESFQFVLLSEPSGLFEIESERD
                     SARRLFGTLKVASGRTAPPVPTDFPLTVGLQDGTNLLSTADCVVHVVAETKLKALLDY
                     SDPVSTKAADTNVAAWIDQLSTNSGRFADITLYDKTLTEYVAARQNVETTWATVIGRL
                     SLLAEAYGHSPVYATNLVQRERLRSALYAGIMALTDWVPMDPAKVLAGGKPIGSDYAD
                     TVFRLNEYGVLSYGTVTHLWTHSDPIGNAGRCMMRSLQEDLRKGDPAARAVHERLVDY
                     LQTCFGNPMNYRYINNPDARWGQLTDTNHTEGAYSDANLNHRVTTWLNLPAIWADYNR
                     PITYVPYWYRGYSADLGFPDTLYMPGWTPHGVLEDFAFWIRTFHRPAHLFLQSGFQPD
                     GSVSHHVDFATDSQLNIYGHPWLVDAIDGYNLLKKVGIDPGSHGFQFIADRYLYTYQR
                     LIYKKDFDFSLTGKLYNGDQSSLVTSIPNYITKLITAKQASTVITNEAELLDLSKRIA
                     TPTNYISGNFPFWVANHLVHRRGDANGEKPYFFAVRMENDRTQGPEDNASPRLAWHAG
                     SGLLQPRVRGDEHGVKTRNNWDWHALPGLTEEWRTDAIPAIDGVDETGGSAYSGMVSD
                     GRYGCAAMEYRSRGTYSAACANKAWFFTETEAVALGSAVSRRIAGQGREIVTTLDQCL
                     WVSNITYAVNGEAAQTIPVGTHPNLTLPMTGPSWIHQGSIGYVIFPQGAQSLYIVGGS
                     NIHHTSTGALTTNLALFAMGHGIDPVAAGLTNYHYVVVPNKTAAEMPAYLTELTNRVE
                     IVANQRSVQGIYDRSLQLLQLAFYTNTSVTASNGLRVAVDGPAMVQLRPTDGGAWSLS
                     VVDPRHDLNLTALNLEINQSLRSGTYAYELPGIYPRPGETIQIVQTQGVARIRVELPD
                     PADDARYNYQAPLYAGAPISRTIPRDYYADWSRSAGITGGPLDDDDHDGLSNLQEYAL
                     GNNPLAAEPRARLSISGSDGVFRFNRRLDRQARGLRYVVRVTSSLINPAWETNGVFEL
                     AVDPASNGFECVTQQIGGLTNRQGFIAFDVDMDQ"
     gene            complement(10990..11868)
                     /gene="ilvY"
                     /locus_tag="PKI68_03670"
     CDS             complement(10990..11868)
                     /gene="ilvY"
                     /locus_tag="PKI68_03670"
                     /inference="COORDINATES: protein motif:HMM:NF008722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HNX52823.1"
                     /translation="MDIRDLNVFLSLADTLHFGKASRACNLSPSALTRSIQRLEEEVG
                     HPLFVRDNRTVTLTPAGVRFQTYARQARQEWQTLQQELHDSPSVSGPLSLYASITAVY
                     SLLPKLLEAYRAACPDVQLELRTGAAEQAVEQVLTGEIDLAVAALPDRHAPQLEFLPL
                     TKIPLVFIAPKQGINSEPPLKRGKLDLSLAPLVLPRSGLSRQRLDQWMKKNNVTPDIT
                     SEVSGNEALIAMVRLGCGIGIVPQLVLEKSPFCDDVRIVENAPVLDPYEVGLCATKRG
                     LARPAVRAFWNLAEKT"
     gene            12125..13603
                     /gene="ilvC"
                     /locus_tag="PKI68_03675"
     CDS             12125..13603
                     /gene="ilvC"
                     /locus_tag="PKI68_03675"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF003557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNX52824.1"
                     /translation="MGQNYFNTLPLRRQLQELGTCRFMDASEFSNGCEYAKGKKIVIV
                     GCGAQGLNQGLNLRDSGLDVSYTLRAAAIKEKRQSWKNATENGFKVGTYEEMLPTADI
                     VMNLAPDKQHTDVVKTVVPLMKKGATFCYAHGFNIVEEGTQIRKDLTVIMVAPKSPGS
                     EVREEYKRGFGVPTLIAVHSENDPNGDGLEIAKALCSAEGGDRAGVLESSFVAEVKSD
                     LMGEQTILCGMLQAGSILCFNKMVEQRIEPGYAAKLIQYGWETVTEALKQGGITNMMD
                     RLSNPAKIQAFQLSEELKGILRPLFEKHMDDIISGEFSRTMMEDWANDDVKLLTWREE
                     TGKTAFEQTPAADVKISEQEYFDKGILMVAMVKAGVELAFEAMVEAGIEPESAYYESL
                     HETPLIANTIARKKLYEMNRIISDTAEYGCYLFAHAAVPMLKEFMNGIGTDVIGKGLV
                     LKDNGIDNRLLVQVNAAIRGHFVEEVGAELRAAMKAMKSLNS"
     gene            13767..14030
                     /gene="rpsO"
                     /locus_tag="PKI68_03680"
     CDS             13767..14030
                     /gene="rpsO"
                     /locus_tag="PKI68_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNX52825.1"
                     /translation="MDTAVKAETRKEYQRSDRDTGSVEVQVALLTKHVEALTVHLQSH
                     KKDNSTRRGLITMVNNRKKLLSYLSRTDNARYKELIQRLGLRH"
     gene            14276..>14811
                     /locus_tag="PKI68_03685"
     CDS             14276..>14811
                     /locus_tag="PKI68_03685"
                     /inference="COORDINATES: protein motif:HMM:NF013318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNX52826.1"
                     /translation="MKNTVKIQFEVGGKPMFFESGLLAQQAAGAVSCGLGDNIVFSAV
                     TATNTPREGVDFFPLQVEYREKFYAAGRFPGGFFKREARPSEKEILTARVTDRPLRPL
                     FPKGYCNDVQINNMLLSCDGQIDTDFLSVNASSAALVISEVPFMGPIAAIRIGRVNGQ
                     FIICPTHEQLAKSDLDLT"
BASE COUNT         3223 a         4062 c         4463 g         3063 t
ORIGIN      
        1 catctcggca aagacctgct ggcgaataca ctgattcaga cgattcaggt tttctcgaaa
       61 gaagaatggc ttgcggcgga gccggatatg accgctccga tctttgatga tcatcaggaa
      121 gtcaaagtga atgtcattga gctgccggac gatgacgcgg cgctgatgaa gatttccagc
      181 gacggcattc tgtcgctgtc gctcgaagaa atgcgcgcga tccgcaatca ctacctgagt
      241 gacgccgtga aggcgcaccg cgccgaactc ggaatgccgg aatggccgac cgatgtggag
      301 ctggagtgta ttgcccagac ctggtcggag cactgctcgc acaagatttt tgccggaacg
      361 gtcgaataca aagacgcaga gaccggcaaa gaagagatta tcaactcgct ctacaaaacc
      421 tacgtcaagg cttcgacgaa gaaggttgcc gaaaagatcg actggctggt ttcggtgttc
      481 accgataacg cgggcgtgat caaattcaac gaagacctgc atctggtcta taaagttgaa
      541 acccacaact cgccgtcggc gctcgatccg ttcggcggat cgatgaccgg tattgtcggc
      601 gtgaaccgcg atccgatggg caccggtatg ggcgcgcagc tggtttccaa cgtctggggc
      661 tactgcctcg gctcgccgtt ttacgataag gaaattcctg ccggtctgat gcatccgcgc
      721 cgtatccgtg acggcgtgca tgccggcgtg atcgcgggcg gcaacgagtc gggcattccg
      781 tggagccgcg gctttgaatg tttcgacgag cgctatctcg gcaagccgct ggtctattgc
      841 ggcacgatgg gcacgctgcc ggtcagcgtc ggcggacgcc cgtccgagcg caaggaagtg
      901 aaagtcggcg actggacggt catgtgcggc ggacgaatcg gtaaagacgg cattcacggc
      961 gcgactttct cgtccgaaga actgcgtacc gaatcgcccg cgcaggcggt gcagatcggc
     1021 gacccgctga cgcaacgcaa actttacgaa tttctgatgg aagcccgcga cctcggtctc
     1081 ttccgctgta tcaccgacaa cggcgcgggc ggcatctcct gctcgttcgg cgaaatgggc
     1141 aaatattccg gcggctgcga gtgcgaactc aaggatgcgc cgctgaaata tgaaggtctc
     1201 cagccgtggg aaattctcgt ttccgaagcg caggagcgta tgacgctggc cgttcagccc
     1261 gaatgccgcg aagcgtttga aaaactggcg aaacagcgcg atgtcgaagc cgcctttatg
     1321 ggcaagtaca acgactccgg ctatttcacc gtaaagtatg acggcaaggt cgtcgccagt
     1381 ctcgatatgg atttcctcta cgaaaccggc tgtccgaaac tgcacatgaa gggcgtctgg
     1441 aagaaaccgg aaattccggc gccggaaaaa gcaaaaggcg gaaaccgcac gaagcagttg
     1501 cgccagatga tgggccgact gaatatctgc tcgcgtgaat acaagagccg catctatgac
     1561 ggcgaagtga agggcctttc cgtcgtcaag ccgttcgtcg gcgtgaacgc cgacgtgccg
     1621 tccgacgcga cggtcatgcg tatcgaatac aacaaagatc gcggcgcgat tctggccgaa
     1681 gggatcaatc cgtggttttc cgatatcgac acctatcaca tgaccgcgtc ggtcatcgat
     1741 gaatccatcc gccgcgtcat cagcgtcggc ggacgcctcg accgcatcgc gattctcgac
     1801 aacttctgct ggcccgatcc ggtgcagtcc tccaaaacgc ccgacggcga atacaagatg
     1861 gcgcagctcg tccgctccaa taaagcgctc tacgactaca ccgtcgcgtt cggcacgccg
     1921 gcggtttccg gcaaggactc ctgcaaaaac gactcgaccc gcggcggcaa gaaaatctcg
     1981 attccgccga cgctgctcat ctcgtcgatc ggccagatcg acgacgtgaa gaaggccgtc
     2041 accatgccgc tcaaagaagc gggcagtctg atctacatgg tcggcgtcac gaagaacgag
     2101 ctcggcgcct cggagtggaa ccgtatgctg gcggaagagc agggcaatcc gtggaactac
     2161 ggcggcgacg tgccgacggt tgatgccaaa gccgcgctcg cgctttaccg cgccatgaat
     2221 caggcgcagg ctgaaggaat tctcctttcg tcgcacacgc cgtcgaaggg cggactcgcc
     2281 gtcggcttcg cgctcatctg catgggcgga cagctcggcg cggaaattga tctggcgaaa
     2341 gttccgacgc ccggtaagcg gaagctcaaa gacgatcaga ttcttttctc ggaatccaac
     2401 agccgcttca tcgtttcggt gaagccggaa aatgcggcgc ggttcgaagc gctctttgcc
     2461 ggcctgccgg ccgcgcagat cggaacggtc acggcggaga agaagctgac cgtcaaaggc
     2521 gttaaaggtg cgacgcgcat cgacaccgat ctcgaaacgc tccgcaagcc gttcaagaaa
     2581 acgctctacg gcatttaatg aatgttctgc tccagcaagg aaacggctgg cttcgttttc
     2641 cggagccggt cgaagtgctg acggcgcgta cgccggacgc agttttccag ctgtttgcgg
     2701 gaaattgact tcgcggagag tttccggcac cctgccggca tggttttcga tctgaaacaa
     2761 aaagagtcgc tgaaaaaaga tctggtttcc tgtctgtcag gcgatcagga aattcagcgc
     2821 attgtgattt tcgggtcgtt cgttggttcg tcttcgccgg aggatatgga tgtggctgtt
     2881 ttccagaaca gcgccgagcc gtatctttct ttggccgtaa aataccgccg acagacccgt
     2941 tcgatatccc gccggattcc gctggatatc atcccgctcc gcgccggggt gacggacgat
     3001 ccgtttctta aggaagtcga acggggcgaa acagtttatg agcgttgaac gttccagatg
     3061 gatcgcttat gccgatgaaa atctggcgat tgcccggttg gcgctcgata gcggatatta
     3121 caacgcctgc ctgcagaatg ttcagcaggc aggcgaaaaa tatctgaaag cccttctgct
     3181 tgcatgcgga ctggaatttc ggaaaacgca cagcattgaa acactcagga accagctggc
     3241 tggcgaggga cttgatgcgg cactgtccga tgaggactgc atattgctgg attcgatcta
     3301 tatgccatcc aaatatccat tcggcagcgt attgccggat tttaaccccg atgcggatat
     3361 ttgcgatcaa tgtatccgaa tcgctgatgc cgtgcgaaaa gcggtctttt cggcaggagt
     3421 ctgatgtttg aggtgctgct ccagcacgga aacggctggc ttcgttttcc ggagccggtt
     3481 gaagtgctga cggcgcgtac gccggaagaa gtattccggt gtttggaaag aatcgaaacc
     3541 agcggcctgt gggccgccgg ttttatttcc tatgaagccg ctggcgcgtt tgacagtgct
     3601 ctgaaaactc acgcaccggg cagtctgccg cttttgcagt ttgggttgtt tgaaaatgtg
     3661 gagcagacat tcctgtctgc tcaaatgcac aggcaggaat gcctgcgcca ctatcagctt
     3721 ggagagtgga aaccatcggt gacgcgcggc gaatactctg aagcgattgc gaagatcaag
     3781 gagcgcatcg cggcgggcga tacctatcag gtgaattaca ccttccggct gaacgcggat
     3841 ttctccggtt cgccgttcga tttcttttgc gacctcgccg ccgcgcagca ggggcgctat
     3901 gccgcgttta tcgaaacccc ggagttcgcc gtctgttccg cctcgccgga gcttttcttc
     3961 gagctcaaag acggcgtaat tacgtcgcga ccgatgaagg gcaccctgcc gcgcggactg
     4021 acggcagagg ccgaccgcgc cgcggctgaa gcgctgaaaa actcggcgaa ggactgcgcc
     4081 gaaaacatca tgatcgtcga tatgatccgc aacgatatcg gtcgaattgc cgaggcgggt
     4141 tccgtggaaa ccgtcagccg tttcgatgtc gaaaaatatc cgaccgtctg gcagctgact
     4201 tcgacagttc agggtaaagt gtgtggtgca ggctttcagt ctgcacatga gcaggcagga
     4261 atgcctgctc cacaaatttt caaagcgctg tttccctgtg cgtcgatcac cggcgcgccg
     4321 aaggcgaaga cgatggagat tatccggggt ctggaaaaaa cgccgcgcgg catttatacc
     4381 ggcgcgatcg gctttattgc gccggacggc gcggcgcagt tcagcgtggc gatccgcacg
     4441 gcggtgattc aggccgggcg cgcggaatac ggaaccggcg gcggcatcgt gtgggattcc
     4501 gatgcggact ctgagtatga cgaggcgctg tcaaaggcgc ggattctcac gcgccgcttt
     4561 ccggagtttc agctgctgga gacgatgctt tgggaagatg gagaaatctt tctgctggag
     4621 cgccatttga agcggctggc cggttcggcg gagtattttg gatttaacct ggatgagtca
     4681 aaagttcgtg accgcctgcg tgatttaaag tccgatgaac cgatgcgggt ccggctgttg
     4741 ctggataaag acgggaggtt cgaagttcaa gcattcaaaa tgaaacgcgg cagggacgcc
     4801 gcgtctacgt tggcaattgc cagagagcct gtcgattcgg gtgatgtgtt tctgtatcac
     4861 aagacgacga accgtgcggt ctatgagcgg gccagggcgg actttccgga agccgatgat
     4921 gtgctgctgt ataacgaacg cggtgaaatc accgaaagct gtattgcgaa tgtggtggtt
     4981 gagctcgacg gccgcaaggt gacgccgccg gtttcctgcg gcctgctggc ggggacgttc
     5041 cgtaatgagc ttttggagcg gggggagatt gaggagcagg ttgtgagcgt ggaagaactc
     5101 agacgtgccg acgcaatctg gctgataaat tctgttcgca aatggcggaa ggtttcgctt
     5161 aactgttcgg cctcatgatg aaaccaaaaa ttttaattat tacgggctac ggagtgaact
     5221 gcgaggcgga gtcttcgcgg gcgtgggaac tggcaggcgc agaaccggtc aaggttcatc
     5281 tgaacgacct gctcgatcat ccggcgcaga tgcgcgattt ccgcggcctg atgtttatcg
     5341 gcggcttttc ctacggcgac cacatgacca gcggtcacgt cttcgcgctg cgcgtcaaac
     5401 atcacctgcg cgaggagctc cagaagttta ttgatgacgg caaaatcatt ctcggcgtct
     5461 gcaacggttt ccagacgatg accaagatcg gcctgctgcc ggggctgaac ggcgaatact
     5521 ttgagccgaa ggtttcgctg atgcagaacg actgcggaca cttccagaat ttctgggtga
     5581 acctgaagtt cgaagcgaac tcgccctgta ttttcaccaa aggcatcgcc ggtatgccga
     5641 tgccggtccg tcacggcgaa ggcaaaattt tcaccacgga taaaaccgtc attgaacaga
     5701 ttgaaaaact cggctgcgcc gccgcgcgct acgcagacgc ggacggcaat ccgacgatgg
     5761 attttccggc caacccgaac ggctcgctga atgccattgc cggactgtgc gacccgaccg
     5821 gccgcatctt cggcctgatg ccgcatcctg aggcctacct ttttcctgag aaccacccca
     5881 actgggacgt gcagaagctg aacggcacgc tgcccgcgca gggactggga ctcaaacttt
     5941 ttgaaaacgg cgtgaagttt ctcgcccgct aactgattcg cagattttta cagacagcgg
     6001 aaatacagca tcggctctgg gagccgatct tccggtgttt gggaaacggc tccggaagtt
     6061 ccggggtttg gaaacaggta ggcttagttc aaaaaggaga agtgttatgg cagtcatcga
     6121 cttcggcggt accaaagaaa ccgtcgtgac ccgtaaagag ttcccgctgg ccaaggcccg
     6181 caaagtgctg aagaacgaaa tcatcgccgt gatcggttac ggcgtacaag gcccggccca
     6241 ggcgctgaat ctgcgcgaca acggctttaa tgtcattgtc ggtcagtcca aaaagttcat
     6301 gcaggactgg aaccgcgccg ttaaagacgg atttgttccc ggcaaaaccc tgttcgaaat
     6361 cgaagaagcg gtacagaagg gaacggtcat tcagtacctg ctttccgacg cggcgcagaa
     6421 actctgctgg ccgcagatta agaagaacct caagcccggc gacgcgctct acttcagcca
     6481 cggcttctcg atcgtttaca aagatctcac caaagtcatt ccgccgaaaa atgtcgacgt
     6541 gattctggtt gctccgaagg gctccggact gaatgtccgc cgcaacttcc tgaccggcgc
     6601 gggcatcaac tccagcttcg gcgtattcca ggacgccacc ggccgcgccg aagagcgctg
     6661 catggcgctg ggtatcggta tcggttccgg ctatctcttc ccgaccacct tcgagaagga
     6721 agtcacgtcc gacctcgtcg gcgaacgcgg tatcctgatg ggcgcgctgg ccggtgtgat
     6781 ggaagcgcag tacagcgtgc tccgtaaaaa cggacacagc ccgtccgaag ccttcaacga
     6841 aacggttgaa gagctcacgc agagcctgat ccgtctggtt gatgaaaacg gtatggactg
     6901 gatgttctcg aactgctcgg ccaccgcaca gcgcggcgcg ctcgactggt cgccgaagtt
     6961 caagaaggcc acgctgccgg tattcaatga cctctacaaa gcggtcaaga cgaagtccga
     7021 agccaaacgc gtactgaaag tctgcggcgc gaaagactac aaagaacgcc tgaccaagga
     7081 actgaaagcc atccacgatt ccgaaatgtg gcaggccggt gcggcctcgc gctcgctccg
     7141 tccgaaaggc gaagccaaag tgattgccaa gggcaccaaa ggtgtcggcg gccgcgccgc
     7201 caactaaagt ctggttccat cagacgacga aacgggctcc tgcaaaggag cccgtttctg
     7261 tccgggcttt ctcaggctga tgacagtgtt ttgtatatgt tttatcctgt tttttgataa
     7321 tatatcggtt ggtttttcca gtgtgtgtcg tgtttgcggg gaaaagatga tgaagcatag
     7381 tcttataatg atcggatccg gactgctgct ggggtttgcg gcggcgcagg ccgctccgct
     7441 gattacgctg aacggcggtg cgctggtgac aaccgaggcg ggtaagccgt tcgttgatcc
     7501 gggcgcgacg gttgtgaatc cggaagctcc cgcgttcgag ccggtggtgc tgaagaccaa
     7561 cggtacggta aagatttata cgcccggcga ttattcgctg acctatacgg cggtgaatgc
     7621 ggcgggcgtg agcaatacgg cggtgcgcac ggtgcgcgtg gtgatcagcc gcgagctgct
     7681 ggccaactat tttgatattg agaccaacgc cccggcgggg ctggaggtga tcggcaaggt
     7741 tcacctgctg gcgaattata atgcgcaccg gtatccgatt ccggagtcgt ttcagttcgt
     7801 gctgctgtcg gaaccgagcg gacttttcga aatcgaatcg gagcgcgatt ccgcgaggcg
     7861 cctgttcggc acgctgaagg tggcttccgg gcggaccgcg ccgccggttc cgacggactt
     7921 tccgctgacg gtggggctgc aggacgggac gaatcttctt tcgacggcgg attgtgtggt
     7981 gcatgtggtg gctgagacga agctgaaagc gctgctggac tacagcgatc cggtcagcac
     8041 aaaggccgcc gatacaaatg ttgcggcctg gattgatcag ctgtccacca acagcggccg
     8101 gtttgccgac atcacgctgt atgacaagac gctcaccgag tatgtcgccg cgcgccaaaa
     8161 tgttgagacg acctgggcga ctgtgatcgg gcgtttgtcc ctactggccg aggcctacgg
     8221 gcactcgccg gtttacgcga ccaatttggt gcagcgtgag cggttgcgct cggcgctcta
     8281 tgccggaatt atggccctca ccgactgggt tccgatggat cccgccaagg tgctggccgg
     8341 cggaaaaccg atcggctccg actatgccga taccgtgttc cgcctgaatg aatatggcgt
     8401 tctcagctat ggcacggtca ctcacttgtg gacgcacagc gacccgatcg gcaatgcggg
     8461 acgctgtatg atgcgttcgt tgcaggagga tttgcgcaaa ggggatccgg ccgcccgggc
     8521 ggttcatgaa cggctggttg attacctgca aacctgtttc ggcaacccga tgaactaccg
     8581 ctacatcaac aatccggatg cgcgctgggg acagttgacg gacacgaacc atacggaagg
     8641 ggcgtactcg gacgctaatt tgaaccaccg cgtgacgacg tggctgaatc tgccggcgat
     8701 ctgggccgac tataaccggc cgattaccta tgtgccgtac tggtaccgcg ggtattcggc
     8761 cgacctgggg tttccggaca cactctacat gcccggctgg acgccgcacg gcgtgctgga
     8821 ggatttcgct ttttggatcc ggacgtttca ccggcccgcc catctctttc tgcaatcggg
     8881 gtttcagccg gacggctcgg tttcccatca cgtcgacttt gccaccgatt cgcagctgaa
     8941 tatctacggt catccgtggc tggtggatgc gattgacggg tataatctgc tcaagaaggt
     9001 tggcatcgac cccggcagcc acggttttca gtttattgcc gaccgttacc tgtatacgta
     9061 tcagcggctg atttataaga aagacttcga ttttagtctg actggcaagc tgtacaatgg
     9121 tgatcaaagc agtttggtca cctcaatccc gaattatatc accaagctga ttactgccaa
     9181 gcaggcctcc acggtcatca caaacgaagc ggagctgctg gatttgagca agcgcatcgc
     9241 caccccgacg aactatattt caggcaactt tccgttctgg gtcgcgaacc accttgtgca
     9301 tcgccgcgga gatgccaacg gcgaaaaacc ctactttttc gccgtgcgca tggaaaacga
     9361 ccggactcaa ggcccggaag ataatgcttc gccgcggctg gcctggcatg ccggcagcgg
     9421 tcttctgcag ccccgtgtgc gcggggatga gcatggcgtg aagacgcgga acaactggga
     9481 ctggcacgcc ctgccggggc tgacggaaga gtggcgtacc gatgcgatcc cggcaattga
     9541 tggagttgat gagaccggcg gcagcgctta ttccggcatg gtttccgacg gccgctacgg
     9601 ctgcgccgcg atggaatacc gttcgcgcgg aacctattcg gccgcgtgcg ccaataaagc
     9661 ctggttcttc acggaaacgg aagccgttgc gctgggaagc gccgtctccc ggcgcattgc
     9721 cgggcaaggg cgggaaattg tcacgacgct ggatcagtgt ttgtgggtct cgaatatcac
     9781 ctatgccgtg aatggcgagg ctgcgcagac catcccggtc ggcacccatc cgaacctgac
     9841 actgccgatg accggaccgt cctggatcca tcagggcagc atcggctatg tcatttttcc
     9901 gcaaggggcg cagtcgcttt atattgtcgg cggcagcaac atccatcaca cctcgacggg
     9961 agcgctcaca accaatctgg cgctcttcgc catgggtcac ggcattgatc ccgtcgcggc
    10021 ggggctgacc aactatcact atgtggtcgt gccgaataaa acggccgccg aaatgccggc
    10081 ctatctgacg gagctgacca atcgcgtgga aattgtcgcc aaccagcgct cggtgcaggg
    10141 gatttatgac cggtctctcc agctgctcca gctcgccttc tataccaaca cttcggtgac
    10201 ggcctccaac ggcctgcgcg tcgcggtgga cggcccggcg atggttcagc tgcgcccgac
    10261 cgacggcgga gcctggtcgc tgagcgtggt cgatccgcgg cacgatctga acctcaccgc
    10321 gctcaatctg gaaatcaacc aaagcctgcg cagcggaacc tatgcgtacg agctgcccgg
    10381 catctatcct cgccccggcg aaacaattca gattgtacag acgcagggcg tggcccgcat
    10441 acgggtcgaa ctgccggatc cggcggacga tgcgcgctac aattatcagg ccccgctcta
    10501 tgccggagcc ccgatcagcc gcaccattcc gcgcgactac tacgccgact ggagccgttc
    10561 ggccggcatc accggcggcc cgcttgatga cgacgatcac gacggcctca gcaatctgca
    10621 ggaatatgct ctggggaata atccgctggc ggccgagccc agagcccgtt tgagtatttc
    10681 cggatccgac ggagtttttc gttttaaccg ccgattggac aggcaggccc gcgggttgcg
    10741 gtatgtcgtg cgggttacca gttcgctgat aaatcccgcc tgggaaacca acggagtttt
    10801 tgagctcgcg gtggatccgg cttcgaacgg ctttgagtgc gtcactcagc agatcggcgg
    10861 actgaccaac cggcagggtt ttatcgcatt tgatgtagat atggatcaat aaagctgagg
    10921 ctccgtcgcc tcgtctacat tgggaaaact ttgcgttctt tgcggctctg cgaggactgt
    10981 cccgttcggt tacgttttct ctgccagatt ccagaaggcg cggacggcgg ggcgggcgag
    11041 tccgcgtttg gtggcgcaga gtccgacttc atagggatcg agcaccggcg cgttttccac
    11101 aatccggacg tcgtcacaga atggactttt ttcgagtacg agttgaggga cgatgccgat
    11161 gccgcaaccg agccgtacca tggcgatgag tgcttcgttg ccggaaactt ccgaggtgat
    11221 gtcgggcgtg acgttatttt tcttcatcca ctggtcgagc cgctgacgcg agagtccgct
    11281 gcgcggaagc acgagcggtg ctagacttaa atccagtttg ccgcgcttga gcggcggctc
    11341 ggagttgatt ccctgtttcg gcgcgatgaa gacgagcggg attttggtca gcggcagaaa
    11401 ttcgagctgc ggcgcgtggc ggtcgggcag ggcggcgacg gcgaggtcga tctctccggt
    11461 gagcacctgt tcgaccgcct gttcggcggc gccggtgcgc agttcgagct gaacgtcggg
    11521 gcaggcggca cggtaggctt cgaggagttt ggggagcagg ctgtaaacgg cggtgatgga
    11581 ggcgtagagc gagagcgggc cgctgacgga cggcgagtcg tgcagttcct gctggagcgt
    11641 ctgccattcc tgccgcgcct gccgggcgta ggtctggaag cggacgccgg cgggcgtgag
    11701 ggtgacggtg cggttgtcgc gaacaaaaag cggatggccg acttcttctt cgagccgctg
    11761 gatggagcgg gtgagcgccg acgggctgag gttgcacgcg cggctggctt tgccgaagtg
    11821 aagcgtgtcg gcgagggaga ggaacacgtt taagtcgcga atatccattg tttgtttaac
    11881 ctgtctgtct gtctgtggtc gcgtattagc tatgaagttt atgaaagatg aactatgaaa
    11941 atggaactca ggcggcgttg gcctgttatg gtaaggctgt cgcagacccg ctttcatact
    12001 tcatctttca tagttcatcc ttttgttgcg taaaacgcaa cgacacattt acatcaaatc
    12061 aatttacgca acgataaacc cccgctacac tgcgcggctt ttaaaccacc aacaggagat
    12121 ttttatggga cagaactatt tcaatacgct tccgcttcgc cgtcagctgc aggagctggg
    12181 cacctgccgc tttatggacg cttcggaatt ttccaacggc tgcgaatacg ccaagggcaa
    12241 aaagatcgtg atcgtcggct gcggcgctca ggggctgaat cagggtttga atctgcgtga
    12301 cagcggtctg gatgtttcct acacgctgcg cgcggcggcc atcaaagaga agcgtcagtc
    12361 gtggaagaac gcgacggaga acgggtttaa agtcggcacc tacgaagaaa tgctgccgac
    12421 ggctgatatc gtgatgaatc tggctccgga taagcagcac acggatgtgg ttaagacggt
    12481 tgttccgctg atgaagaagg gcgcgacgtt ctgctatgcc cacggtttta atatcgtcga
    12541 agaaggcacg cagatccgca aggacctgac cgttatcatg gttgcgccga aatcgccggg
    12601 ctcggaagtc cgcgaagaat ataagcgcgg cttcggcgtc ccgacgctga ttgctgtaca
    12661 cagcgagaac gatccgaacg gcgacggttt ggaaatcgct aaagcgctct gctccgccga
    12721 gggcggcgac cgcgccggcg ttctggaatc ctctttcgtg gccgaagtga agtccgacct
    12781 gatgggcgag cagacgattc tctgcggtat gctgcaggcc ggttcgattc tctgcttcaa
    12841 caagatggtt gagcagcgca ttgagccggg ttatgccgcc aagctgattc agtacggctg
    12901 ggaaacggtt accgaagcgc tcaagcaggg cggcatcacc aatatgatgg accgtctttc
    12961 caacccggcc aagattcagg cgttccagct gtcggaagag ctgaaaggaa tcctgcgtcc
    13021 gctgttcgag aagcacatgg acgacattat ttccggcgag ttttccagga cgatgatgga
    13081 agactgggcg aacgatgacg tcaagctgct gacctggcgc gaagaaaccg gcaagaccgc
    13141 gttcgaacag actccggccg ccgatgtgaa aatcagcgag caggaatact tcgacaaagg
    13201 catcctgatg gttgcgatgg tcaaagcggg cgtcgagctg gcctttgaag cgatggttga
    13261 agcggggatt gaaccggaat cggcctatta cgagtcgctg cacgaaacgc cgctcatcgc
    13321 caacacgatt gcccgcaaga agctctacga aatgaaccgg atcatttccg atacggccga
    13381 gtacggctgc tacctctttg cacatgccgc cgtaccgatg ctcaaggagt tcatgaacgg
    13441 catcggcacc gacgtaatcg gtaaagggct ggtgctgaag gataacggta tcgacaaccg
    13501 cctgctggtt caggttaatg ccgccatccg cggccacttc gtcgaagagg tcggcgccga
    13561 actgcgcgcc gccatgaagg cgatgaagtc gctgaacagc tgagcggaat cgaacgcaga
    13621 gaaggtccgg ggggattaat ccatcgcccc gttccgctct gttttttatt ggatattgca
    13681 gtttacaagg tcggagggcg tctatatatt gtcgcgctca actatagaag tagcgaaaaa
    13741 gaagagagtt aagaaggagc ccgtaagtgg atacagcagt taaggcggaa accagaaaag
    13801 aatatcagcg cagcgaccgg gataccggat ccgttgaagt gcaggttgca ctgctgacga
    13861 agcatgtaga agcactcacc gtgcatctcc agagccataa aaaagacaac agcacccgtc
    13921 gcggattgat caccatggtc aataaccgca agaagctgct cagctatctg tcgagaacag
    13981 ataacgcgcg ctacaaagag ctgattcagc gccttggttt gcgccactaa gaagacgagg
    14041 acggccccgt ttacgacggg gctgttttgt ttcagccgct tccgcggcag ttattcgtca
    14101 atggttattc ggtaatagtt ggaaacgtca cttcaaaaag agcgtttctc cgaataacaa
    14161 acgaaaataa ctaataacta aaatcccggt actgcggatt ttatccacct ccagagacac
    14221 gcatccggtc tcattgggct cccggcagtc atggatgcag aaggaaaaga aagaaatgaa
    14281 aaatacagtc aaaatacagt tcgaagtcgg cggcaagccg atgttcttcg agtccggcct
    14341 cctcgcacag caggccgccg gcgcagtcag ctgcggtctg ggcgataaca tcgtcttctc
    14401 cgccgttacc gccaccaata ccccccgtga aggcgtggat ttcttcccgc ttcaggttga
    14461 ataccgcgaa aaattctacg ccgcaggccg tttccccggc ggatttttca aacgtgaagc
    14521 ccgtccgtcc gaaaaagaaa ttctgaccgc ccgcgtcacc gaccgtccgc tccgtccgct
    14581 cttcccgaaa gggtattgca acgacgtgca gatcaacaac atgctgctca gctgcgacgg
    14641 ccagatcgac accgactttc tcagcgtcaa cgcctccagc gccgcgctgg tgatctctga
    14701 agttcccttc atgggcccga ttgccgcgat ccgtatcggc cgcgtgaacg gacagttcat
    14761 catctgcccg actcacgaac agcttgccaa gagcgatctc gacctgacct a
//
LOCUS       DAOPZO010000034        14622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_4472_length_14622_cov_8.004943, whole genome shotgun sequence.
ACCESSION   DAOPZO010000034 DAOPZO010000000
VERSION     DAOPZO010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 14622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14622
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4472_length_14622_cov_8.004943"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..123
                     /locus_tag="PKI68_03690"
                     /pseudo
     CDS             1..123
                     /locus_tag="PKI68_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611551.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(299..2698)
                     /locus_tag="PKI68_03695"
     CDS             complement(299..2698)
                     /locus_tag="PKI68_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52827.1"
                     /translation="MQRVRLLFSLLLIGAFSARAEGPINIGGIYPHLTMRNNQGECGT
                     GAVVPWQGSLWAITYGPHRPQGSDDKLYEITPALEQKIFKGSVGGTPADRMIHRESNQ
                     LLIGPYLIDAQKNIRVIPPSVMYGRLTGMARHLTDPASKVYYATMEQGLYEVDVNTLA
                     VKCLIRDRNKGAPAVGLVTKIEGAHGKGLYSGQGRLIFSNNGELSNAAMKDPTAVAGA
                     LAQWFGQDDWQLVRRNQFTEITGPGGIYGSSNPDKDPVWAMGWDARSVILALLENGEW
                     SYYRLPKGSHSYDGAHGWNTEWPRIREIGETNLLATMHGTFWRFPANFSKEHSAGIAP
                     RSNYLKVIGDFCRWNDRIVFGCDDSAEKEFLNKRDFKAAKGGPLLSNSNLWFVDPAQI
                     DHIGPVIGRGSVWLRDNVAAGALSDAYLFDGYDYRQLHLKHTSATPVVFTLEVDKLGN
                     NQWTALKTVTVPANGALSLLFAEKEKGAWVRLKTDVAATGVTALFQYRNRDLRSTANA
                     ALFDGLATAQKPAQVSGVMRSLGYNKFGVAAMDAQGNPAGYYEMNQEMKLVPGSKTND
                     YENVLTGAKVTGAGFEEDEASFSITEDGQKWRLPKSAGAARVSGARLCREVATERDLL
                     NLGGTFYEVPARNAQGMAKMRPVATHNLAINDFCSHNGMILFSGIAADAKNERIFRSE
                     DGKAAVWAGVIEELWQLGKPRGVGGPWKNSAVTANKPSDPYLMTAYDKKSVELTASTD
                     AVIKLEVDVDGTGLWIPYQSFSLKAGKAVTHSFPEGFSAYWVRAVSDTATTASVTFTY
                     E"
     gene            2828..3379
                     /locus_tag="PKI68_03700"
     CDS             2828..3379
                     /locus_tag="PKI68_03700"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX52828.1"
                     /translation="MTDTQQIILRVRSGDIEAYGELVRIHQAGVRACLLVRLQDATAA
                     DDLAQEAFLIAYRKLADFDAQRDFGPWVRSIAIRCLQNHLRKKRPYSVGAAAELEQLV
                     DSAVERLHPVEHPETEQQLAALQHCLEQLDSSQRTFIERRYLLKQSIDEIKTAAGLAH
                     STVTMRLHRLREELRTCIEARTT"
     gene            3376..>4044
                     /locus_tag="PKI68_03705"
     CDS             3376..>4044
                     /locus_tag="PKI68_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52829.1"
                     /translation="MTEPPSQLIARYLAGEPVADELLALCRTCPAAADELARHIALER
                     LLRAELSRDQRNALLPEEIIAQLNYDAQAELFAGRITTRLQQMRTLRIRRRRTALWAL
                     AAGVAVLLTGSFWLRSPGPSFARLIAAEAAEWKGSGIALGAPVGNRPVTLTQGFIQLR
                     FNHGTLLVVEAPARFTIRGEKRVRLDYGRAVATVPQAGHGFVIDSPDAEVIDLGTQFG
                     VDVNS"
     assembly_gap    4045..4144
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4145..4831
                     /locus_tag="PKI68_03710"
     CDS             <4145..4831
                     /locus_tag="PKI68_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNX52830.1"
                     /translation="RGTQCRPSGRGMDIQAGLGRFATTATNATESDLPRWITGVFGQA
                     LQFSGRGAYVDTAFPGIAGTAPRTVTFWTKVPTNATLGYAALGWGTFSTGQVWQISLN
                     PNAREGLAGRLRVGILPGMAIGAKDLRDGKWHHIAVVLYPSTAEQPANLRYHVLLYVD
                     GELEPTTRKSLQNIQTVTRASDAAPLRIGCSADMRPPTFSGNLDELYIFDQALNAAEI
                     RRLVIENRID"
     gene            complement(4906..6357)
                     /locus_tag="PKI68_03715"
     CDS             complement(4906..6357)
                     /locus_tag="PKI68_03715"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNX52831.1"
                     /translation="MKTFVWIAGLSALMLLSAPVQGQESATTNSTVPAPAKKASAKKA
                     AAKPASGGSATKTAEAQGQHDELGTGALENDAGGLSNPHPGAQWFPEAGFGLFIHWTM
                     SAVHGGADASWSMLANKSWLGDGTITPNEYWGLMKRWKADQFDMDKTLKAAKEAGFKY
                     AVFVTKHHDGFTFWPTEYGEIGTRQTFAGRDFVKEFVDACRKYDLKVGLYYSPPDWYF
                     DREYRSFSLKGPYLDVNHKPSTKKPKPAGHDEARAELVRNHVRELLTRYGKIDLIFFD
                     GGRAEMPNSEIRKLQPGIVINKRNKDVQDKGDYGDSEGKPPEVRFKGWFEFNDTCWPS
                     AHWAYSASDNMDDASTVLCNLIKMRAWGGNYLANVGPCGSGEVLPEVYAVWREIGQWM
                     AHSGESIYGVKGGSYPEKATVPVTVKDNVAYCFALPPHQGYVTLQDVKAPKSVTLLRT
                     KTPLQYEYTNGVMRLQLPPALRTRLPDVVKVEF"
     gene            6537..8822
                     /gene="rnr"
                     /locus_tag="PKI68_03720"
     CDS             6537..8822
                     /gene="rnr"
                     /locus_tag="PKI68_03720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNX52832.1"
                     /translation="MTNPTLEQRILTFLNQPKYQPLRRPELAKALRLRDTERNKLRQA
                     LINLEKQGKVACLRKNRWAAAGKTTAVKTITGSVRVMEKGFGLFTPDEGGEEIYIARD
                     DLKCALHEDRVAIELFPKAAAEKRGGGKNIQFGNREGRVVEILERKNSEVVGLLRRTP
                     YYAYVIPDNLRLIQDVRVSEWESGLEQTPENHKVVIHLDDWTDPFKPLTGVVIEDLGH
                     RDDPNVEMQCILRAHGFRQNFSDEVLAEAAQMPHELRPEDYHNRRDLRQELTFTIDPE
                     TARDFDDAISLEKAPGGWKLSVHIADVAHFVPRGSVIDREALHRGNSIYLVDRVVMML
                     PTELTTRICSLNPHVDRLAHTVEIMLDKSGEMTGAQSFRSIIHSDARLTYEQAHALFQ
                     GQTDHGVPPHVAAVLQQLLPLTRAIRARRFAAGSLEINTPQIKCLLDKNGKVSSITKG
                     EAKEAYQLIEECMLLANVAVARMLKEAQRPAIHRIHEEPDEDQWAQMGAELQALGINS
                     IPLTRSDINKVMEKIEDTPLEYTGSLAVLRNLKRAGYSAEPKGHFGLAFDDYVHFTSP
                     IRRYPDLVIHRQLTALEEQKASPYRTKDIESIAAQCTRTETEADAAEKESIELRRVEY
                     YNNLLYKGETGPYSGCIIRILNKGLIVELTDTIQRGLVPFSSITDDRYDVNAAKTRAT
                     GQRFRKLFKIGDMLEVDLVKVDMARKFIDFQIAGQKEASKFMPGRKKAGPPRSKQKKQ
                     QVTLTGNRPHGPKQQKRRKRK"
     gene            8826..9683
                     /locus_tag="PKI68_03725"
     CDS             8826..9683
                     /locus_tag="PKI68_03725"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HNX52833.1"
                     /translation="MHSITNGILNVYVKSHGAELCSLKDSGGNELLWQADPAVWNRHA
                     PVLFPIVGKLLDGRYTLNGKTCELPPHGFARDMDFALIERRSSDLVYQLLPTAETRKS
                     YPFEFDFRIAYHLTGQTLGIRYTVKNGSKETMPFSVGAHPAFMLPGPIDECFLDFEKN
                     ETLYARLLSSKGLLSNETVPVLKDSNRLPLSKTLFDRDALIFLDTASDRITLGAKNSS
                     RRLTVEFPGFPQLGIWAKPGAPFVCIEPWYGHADPEQPYGDIMNKPGIRQLPAGGTFT
                     CEHRILVKE"
     gene            9724..10437
                     /locus_tag="PKI68_03730"
     CDS             9724..10437
                     /locus_tag="PKI68_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52834.1"
                     /translation="MIVSNNGKAYNPRLMHIIFKNGLAAAKKNIGPGLLLQGFALALL
                     LLYYFHAPTRQLLMTIPEIKARMGISFPLLMTAFSGGILPFIFLAFRKEIPAGRYIPH
                     LLFLTGFWAFNGMMVDLLYQGQALLFGDQADFATLIKKITVDQFVFCPLYSAPVTAVA
                     MHWKNCDFSWQKTKAELSARVFAFEMLSVLISIWGVWLPTVFILYSLPLALQFPLFNI
                     ILCFWSLLLSALNSKPQSA"
     gene            complement(10439..11263)
                     /locus_tag="PKI68_03735"
     CDS             complement(10439..11263)
                     /locus_tag="PKI68_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNX52835.1"
                     /translation="MKKHPAFHRTELLIGADALQRLADTRVILFGVGGVGSWCAEALI
                     RSGVGHLTIVDNDVICVTNINRQLQATSKNVGKPKVLALQERLLDIHPDADITALQKV
                     YNYETRAEFNLSGYDYVIDAIDSLSHKVDLIASAMESGVTLFSAMGAASKLDATTIRV
                     DSIWKSRGCRLAHFVRKRLRSRGVTGDCLCVYSEEFLPAFEAVKAACGSAQCFCPKQL
                     DDEGNEIPSDVWCHQKAQINGSMVHITGAFGFNLAGLVVQDIVRKAFCDDSTSQPE"
     gene            complement(11260..12192)
                     /locus_tag="PKI68_03740"
     CDS             complement(11260..12192)
                     /locus_tag="PKI68_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52836.1"
                     /translation="MSSERIERILNSGRRAVLVTTGGGSSALNAILTTPGASRFVRAA
                     HIPYSLEALEEFLGEKPEQAVSPATASKLAEKAFHSGVGTSLPIGIGCTAALQTDHER
                     RGEDRAFISFVTAGVERLYALYFSKTSRAEQEALLSGWLLVLIAQAVGAERGLMLPGS
                     FNPVHQGHRNLLKAAEELTGLRGVFELSCANVDKPAIEEADALRRAAAIKDVPVALTH
                     APRFIQKAQLFPKTAFAVGFDTAIRFVKDYSPDDWKKFQTLETRFLVAGRQFGGSFQT
                     LKNMELPAGFESLFEAVPESKFREDISSTALRIS"
     gene            complement(12189..12797)
                     /locus_tag="PKI68_03745"
     CDS             complement(12189..12797)
                     /locus_tag="PKI68_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX52837.1"
                     /translation="MKNNLKALCGVFAAAFLLVGCSRAEVEAKVENTPEPAKETKVMI
                     LMKTTKGDIKLELDAAKAPKTVANFLKYVESGHYKDTIFHRVISNFMIQGGGMTANMN
                     QKKAPYTVENEANNGLKNVRGSIAMARTSDPHSAGAQFFINVVDNGFLDFKAPTQQGW
                     GYCVFGKVVEGMDVVDAIRAVPTAPGDVPRETVTITEVSVIK"
     gene            12869..13729
                     /gene="purU"
                     /locus_tag="PKI68_03750"
     CDS             12869..13729
                     /gene="purU"
                     /locus_tag="PKI68_03750"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNX52838.1"
                     /translation="MKNEASAILLISCPDRQGLVCAVTDFLMRHNGNIIDLEQHVDTE
                     ERVFFMRVEWELNGFALPRDQIGEKFSLIASRFEMKYELHFSDYKPRLAIFVSKFPHC
                     MLDLLARQSSGDLNAEVPLIISNHTDMEPVARMHGIDFHCIPVSKENKAEQEARQMEL
                     LKQYSIDVIVLARYMQVLSDSFCRNYPNRIINIHHSFLPAFVGAKPYHSAHQRGVKIV
                     GATSHYVTGDLDEGPIIEQEVMRVTHRDSVESLIRKGRDLEKLVLARAVWDHLQNKVL
                     VYKNRTVVFG"
     gene            13732..>14622
                     /locus_tag="PKI68_03755"
     CDS             13732..>14622
                     /locus_tag="PKI68_03755"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNX52839.1"
                     /translation="MTKARRLRRRPLETAAFRAGLAVVPRLPRRALLGLAWLGGNLAF
                     LFDRRGRRVGLANLDLAFGDAKSAAEKRAILRAAFITMTRTFLDVIWFGTDSKNRLNR
                     YVELDSSVQQFLCPKNQICVTAHFGNWEVIGQMMAIHGFPLHSIAMPVKNPDVDRLLI
                     ARREVTGQKIIPRDGALRKLLGVLRNNGKTAFLVDQNTKESEGGIWADYFGLPVPVTP
                     APAALAVKTGSKILIGFCAPLSGGRYRVYVTETIQPPENAGEETTRELTRQILSAIER
                     EVSKHPEHWLWMYKRWKKIYR"
BASE COUNT         3116 a         4511 c         3997 g         2898 t
ORIGIN      
        1 gcgcaaaaac gagcgctgga gtatggactt cgtacagctc agggtcgaaa actgcggcgt
       61 ctgaatatcg cggatgactt cacgcggaaa tgtctttgga tcgaacccga tacgtcgcta
      121 tcgctgtgca acagaaagtg gacaacagcg cgccgtagta gccgacgaca gcgccagttc
      181 cgataatggc aaaacgtttc atcggcggga aagtgccata aaaaaaaccg ccggggaaaa
      241 ccccggcggt tggttgaact ccaagcggcc gctgtttcca gtggccgccc ggcggagact
      301 attcgtaggt gaatgtcacc gaggcggttg tcgccgtatc gctcacagcc cgcacccagt
      361 aggcgctgaa gccttccggg aagctgtggg tgaccgcttt gccggccttc aggctgaagg
      421 actggtaggg aatccacagg ccggtaccgt caacatccac ttcgagtttg atgactgcat
      481 cggtcgaggc ggtcagctcg acgctcttct tgtcgtaggc cgtcatcagg tagggatcgg
      541 acggcttatt ggccgtcacc gcactgtttt tccagggacc gcccacgccg cgcggtttgc
      601 cgagctgcca gagttcttca atgaccccgg cccaaacagc agctttgccg tcttcgctgc
      661 ggaagatgcg ttcgttcttc gcatcagcgg cgatcccgct gaagagaatc atgccgttgt
      721 gcgagcagaa gtcgttaatc gccagattat gcgtcgcaac cgggcgcatt ttcgccatac
      781 cctgcgcgtt gcgcgccggc acttcgtaga aggttccgcc gagattgagc aggtcgcgct
      841 cggttgccac ttcgcggcag aggcgcgcac cggagacccg cgctgcaccc gctgatttcg
      901 gcagacgcca tttctgaccg tcttcggtga tgctgaagga ggcttcatct tcttcaaagc
      961 cggcgccagt taccttcgca cctgtcagga cgttttcgta gtcattggtt ttggaacccg
     1021 gcaccagctt catctcctga ttcatttcgt agtagccagc cggattgccc tgtgcatcca
     1081 tcgcggccac gccgaacttg ttgtagccca gactgcgcat cacgccggaa acctgcgccg
     1141 gcttctgtgc cgtagcgagg ccgtcgaaga gcgcggcatt ggctgtagaa cgcaggtcgc
     1201 gattgcggta ctggaagaga gccgtcacgc cggttgctgc cacgtcggtt ttcaaacgca
     1261 cccaggcgcc tttttctttc tcagcgaaga gcaggctgag agcaccgttt gccggaaccg
     1321 taaccgtttt gagcgcggtc cactggttgt tgcccagctt atcgacttcg agggtaaaga
     1381 ccaccggcgt agcgctcgta tgcttcaggt gcagctggcg atagtcgtag ccgtcaaaga
     1441 ggtaggcatc gctgagcgcg ccggccgcaa cattgtcgcg caaccagacg gagccgcgcc
     1501 caatgaccgg tccgatatga tcaatctgcg ccggatccac aaaccagagg ttggagttag
     1561 acagaagcgg tccgcctttt gcggctttga agtcgcgctt gttcaggaat tctttctcgg
     1621 cggaatcgtc gcaaccgaaa acgatgcggt cgttccagcg gcagaagtcg ccgatcacct
     1681 tcaggtaatt ggagcgcggt gcaatgccgg ccgaatgctc cttggagaag ttcgcgggga
     1741 agcgccagaa ggtgccgtgc atggttgcca gcagattggt ttcgccgatt tcgcggatgc
     1801 ggggccattc ggtattccag ccgtgcgcgc cgtcatagct gtggctgcct ttgggcaggc
     1861 ggtagtagct ccattcgccg ttttccagca gagccagaat caccgaacgc gcgtcccagc
     1921 ccattgccca caccggatct ttgtccggat tcgagctgcc atagatgccg cccggacccg
     1981 tgatttcggt aaactgattg cgccggacca gctgccagtc gtcctgacca aaccactggg
     2041 ccagtgcacc ggctaccgcg gtcgggtctt tcattgcggc gttcgacagc tcgccgttgt
     2101 tcgagaagat cagacggccc tgccccgaat aaaggccttt gccatgggcg ccttcgattt
     2161 tcgtcaccag accgaccgcc ggtgcgcctt tgttccggtc ccgaatgaga cacttgacgg
     2221 ccagcgtatt cacatcgacc tcgtagaggc cctgttccat ggtggcgtaa tagactttgc
     2281 tggccggatc ggtcaggtgg cgggccatgc cggtcaggcg gccatacatc accgacggag
     2341 gaatcacgcg gatgtttttc tgcgcatcaa tcagataagg gccgatcagc agctggttgg
     2401 attcgcgatg gatcatgcgg tcggccggcg tgccgccgac gctgccttta aagatcttct
     2461 gttccagagc cggcgtaatt tcatacagct tatcatccga gccttgcggc cgatgcggtc
     2521 cgtaggtgat cgcccacagg ctgccctgcc acggcacgac cgcgccggtg ccgcattcgc
     2581 cctgattatt ccgcatcgtc aggtgcggat agataccgcc aatattgatc gggccttccg
     2641 cacgtgcaga aaacgcaccg atcagtaaca gtgaaaacaa caaacgaact cgttgcataa
     2701 cgtcctttct gggttgggtt cggatgataa ttgtcgctgc tggacgggac gacacatttt
     2761 tttgcagaat ttatccgccg aataaacatt gtgtcgaacc ggccttggta acaatactgg
     2821 ttaagccatg acagataccc aacagataat cctccgcgtg cggagcggcg acatcgaagc
     2881 ctacggcgag ctcgtgcgca tccaccaggc cggcgtgcgc gcctgcctgc tcgtgcgcct
     2941 gcaggacgcg accgcggcgg acgatctggc ccaggaggcc ttcctgatcg cctaccgcaa
     3001 actggctgac tttgacgcgc agcgggactt cggcccctgg gtccgctcca tcgccatccg
     3061 ctgcctgcag aaccacttgc gcaaaaaacg gccctacagc gtcggcgcag ccgccgaact
     3121 ggaacagctg gtcgactccg ccgtcgagcg gctccacccc gtcgaacacc ccgaaaccga
     3181 acagcagctg gcggccctgc agcactgcct cgagcagctc gacagcagcc agcgcacctt
     3241 catcgagcgg cgttacctgc tgaagcagtc catcgacgaa atcaaaaccg ccgctgggct
     3301 cgcccactcc accgtcacca tgcggctcca ccggttgcgt gaagagctgc gaacctgcat
     3361 cgaggcccga accacatgac cgaaccgccc tcccaactga ttgcccgcta tctcgccggc
     3421 gagcccgtcg ccgacgaact gctggcgtta tgccgcacct gtccggcggc ggccgacgaa
     3481 ctggcgcggc atattgccct cgaacgcctg ctgcgcgccg aactctcccg ggaccagcgc
     3541 aacgcactgc ttccggaaga aatcatcgcg cagctgaact acgacgcaca ggccgaactc
     3601 tttgccggcc gaatcaccac ccgcctccag cagatgcgca ccctgcgtat ccggcggcgg
     3661 cgcacagcgc tctgggcgct ggccgccggc gtggcggtgc tcctgaccgg cagcttctgg
     3721 ctgcgcagcc ccggcccctc tttcgcccgc ttgatcgccg ctgaagccgc cgaatggaaa
     3781 ggctccggca ttgcactggg cgcaccggtc ggcaaccgtc cggtcaccct gacgcagggc
     3841 ttcatccagc tgcgcttcaa tcacggcacc ctgctcgtcg tcgaagcccc cgcgcggttc
     3901 accatccgcg gcgaaaaacg cgtacggctc gactacgggc gcgcggttgc cacggttccg
     3961 caggcgggac acggctttgt cattgatagt cctgacgccg aggtcatcga cctcgggacc
     4021 cagttcggcg tcgatgtaaa ttcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnncgcggc acgcaatgcc gtccgtcagg gcgcggcatg gatatccagg ccgggctggg
     4201 ccgcttcgcc acaacggcaa caaatgccac ggaatctgat ctgccgcgct ggattaccgg
     4261 cgtgttcgga caggccctgc aattcagcgg ccgcggagcc tatgtcgaca ccgccttccc
     4321 gggaatagcc ggaaccgctc cgcgcaccgt cacgttctgg accaaggtgc cgaccaatgc
     4381 caccctcgga tatgccgcac tgggctgggg caccttctcg accggtcagg tctggcagat
     4441 ttcgctcaac ccgaatgcaa gagaaggact tgccgggcgt ctgcgcgtcg gcattttgcc
     4501 ggggatggcg atcggcgcga aagacctgcg ggatgggaag tggcaccata tcgcggtcgt
     4561 cctctatcct tcgaccgcgg agcagcctgc caatctgcgc taccacgtcc tgctgtatgt
     4621 cgacggagag ctggaaccga ccacccgcaa gtcgctccag aacattcaaa cagtcacccg
     4681 ggccagcgat gccgctcctt tacggatcgg ctgcagtgca gatatgcgtc cgccgacctt
     4741 ttcgggaaac ctcgatgaac tgtatatttt tgatcaggcc ctgaacgctg cagaaatccg
     4801 caggctcgta atcgaaaacc gcatcgacta aggccagcta ggccataaaa aaacgccgac
     4861 ccgcacaggg cggatcggcg cagggcgtcg cgaaaaccta cgctcttaga attcgacttt
     4921 cacaacatcc ggcaggcggg tccgcagcgc cgggggcagc tggagacgca tcacgccgtt
     4981 ggtgtattcg tattgcaacg gtgtcttcgt gcgcagcagc gtcacactct tcggcgcttt
     5041 gacgtcctga agcgtgacat agccttggtg cggcggcaga gcaaaacagt acgccacatt
     5101 gtccttcaca gtcaccggca cggtggcctt ttccggataa gaaccgccct tgacgccata
     5161 aatcgattcg ccggaatgcg ccatccactg accgatttcc cgccagacgg catagacctc
     5221 gggcagaact tcgccggagc cgcaggggcc gacgttggcc agatagttgc cgccccaggc
     5281 gcgcattttg atcaggttgc acagcactgt agaggcatca tccatgttgt cgcttgcgga
     5341 ataggcccag tgggctgaag gccagcaggt atcattaaac tcaaaccagc ccttgaagcg
     5401 cacctccggc ggcttgcctt ccgagtcacc gtaatcgccc ttgtcctgaa catccttatt
     5461 gcgcttgttg atgacgatgc cgggctggag tttgcggatt tcactgttcg gcatttcggc
     5521 cctaccgccg tcgaaaaaga tcagatcgat ttttccgtag cgcgtcagca gttcccgcac
     5581 atggttccgg acgagttcgg cccgcgcttc gtcatgcccg gccggcttgg gctttttcgt
     5641 cgacggcttg tgattcacat ccaaataagg tcctttcaag ctgaaggaac ggtattcgcg
     5701 atcgaagtac cagtccggcg gcgagtaata gagaccgacc ttcaaatcat atttccggca
     5761 cgcatcgaca aactccttca caaaatcgcg cccggcgaag gtctgtctgg ttccgatttc
     5821 gccgtactcc gtcggccaaa acgtgaagcc gtcgtgatgc ttggtcacga acacagcgta
     5881 tttgaagccg gcctctttcg ccgccttcaa ggtcttgtcc atatcgaatt gatcggcttt
     5941 ccaccgcttc atcaaccccc aatactcatt gggagtgatc gttccatcgc cgagccagga
     6001 cttattggcc agcatgctcc aggaggcatc cgccccgccg tgtaccgccg acatggtcca
     6061 gtgaatgaaa agtccaaagc cagcctccgg aaaccactgc gctccgggat gaggatttga
     6121 cagaccgccc gcatcatttt ccagcgcacc ggttcccagc tcgtcgtgct ggccttgtgc
     6181 ctcggcggtt ttagtcgccg atccgccgga cgccggcttc gcagccgcct tttttgcgga
     6241 cgcttttttt gcaggagcgg gaaccgttga attggtggtt gccgactcct gtccctgcac
     6301 cggggcactg agcagcatca gtgcactcag acccgctatc catacgaatg tcttcatcaa
     6361 tttcatgcct cctgtttgcc tggtctcatc accgcgagaa caattagtcg gactgcgcga
     6421 tccggtcttt cgttcaagag aattgttact caacgcgagg tgacacgata aatgagaaac
     6481 gaaataccgc aagcctaatg gattgtattc tgcaccacac tcggtaatct ccccgcatga
     6541 caaatccaac gctcgaacag cgcatcctga cgtttctgaa ccagccgaaa taccagccgc
     6601 tccgccgccc cgagctcgcc aaggcactgc ggctgcggga taccgagcgc aacaaactgc
     6661 gacaggcgct gatcaacctc gaaaaacagg gcaaagttgc ctgcctgcgc aaaaaccgct
     6721 gggcggcggc cggaaagaca accgcggtta aaaccatcac cggctcggtg cgcgtgatgg
     6781 aaaaaggctt cggcctgttc acccccgacg aaggcggcga agaaatctat atcgcccgcg
     6841 acgatttgaa gtgcgcactc cacgaagacc gcgtggccat tgaactcttt ccgaaagcgg
     6901 cggcggaaaa acgcggcggc gggaagaaca tacagttcgg caaccgtgaa ggacgcgtgg
     6961 tcgaaatcct cgaacgcaaa aacagcgaag ttgtcggtct gctgcgccgc accccgtact
     7021 atgcctatgt cattccggac aatctgcgcc tgattcagga cgtccgcgta tctgaatggg
     7081 agagcggact ggagcagacg cccgaaaacc ataaagtggt catccatctg gacgactgga
     7141 ccgatccgtt caagccgctc accggcgtgg tgatcgaaga cctcggacac cgcgacgatc
     7201 cgaacgtcga aatgcaatgc atcctgcgcg cccacggctt ccgccagaat ttttccgacg
     7261 aggtcctcgc cgaagccgcg caaatgccgc atgagctgcg gcccgaggac tatcacaacc
     7321 gccgcgacct gcgccaggag ctgacgttca cgatcgaccc cgaaaccgcg cgcgacttcg
     7381 acgacgccat ttcgctggaa aaggcccccg gcggctggaa gctttcggtg catatcgccg
     7441 acgtcgccca ctttgtaccg cgcggttcgg tcatcgaccg ggaagcgctg caccgcggca
     7501 acagcatcta cctcgtcgac cgcgtggtga tgatgctgcc gaccgagctg accacgcgca
     7561 tttgcagcct caacccgcac gtcgaccggc tggcccacac cgtcgaaatc atgctggata
     7621 aaagcggcga gatgaccggc gcgcaaagct tccgctcgat catccactcc gacgcgcgcc
     7681 tgacctatga acaggcccac gcgctgtttc agggccagac cgatcacggc gtgccgccgc
     7741 atgtggccgc tgttctccag cagctgctgc cgctcacccg cgccattcgc gcacggcgtt
     7801 tcgcggccgg ctcgctcgaa atcaatacac cgcagattaa atgtctgctc gataaaaacg
     7861 gcaaggtttc ctccatcacc aaaggcgaag cgaaagaagc ctatcagctc atcgaagaat
     7921 gcatgctgct ggcgaacgtc gccgtcgcac gcatgctgaa agaagcccag aggcccgcga
     7981 ttcaccgtat ccacgaggag ccggacgaag accagtgggc gcagatgggc gcggaactgc
     8041 aggccctcgg catcaactcc attccgctga cgcgcagcga catcaacaag gtgatggaaa
     8101 aaatcgaaga cacgccgctg gaatacaccg gcagtctggc ggttctgcgc aacctcaaac
     8161 gcgccggcta ttccgccgaa cccaaaggac atttcggact cgccttcgat gattatgttc
     8221 acttcacatc gccgatccgc cgctaccccg atctggtgat tcaccgccag ctcacggcgc
     8281 tggaagaaca gaaagcgtcg ccataccgga ccaaagatat tgagtccatc gccgcgcagt
     8341 gtacccgcac ggaaaccgaa gcggatgccg ccgagaagga aagcatcgaa ctgcgccgcg
     8401 tcgagtatta caacaatctg ctctacaaag gcgaaaccgg cccctactcc ggatgcatca
     8461 tccgcattct caacaagggg ctgattgttg aactcaccga caccattcag cgcggactgg
     8521 ttccgttctc ctccatcacc gacgaccgct acgacgtcaa cgccgcaaaa acccgcgcca
     8581 ccggccagcg gttccgcaaa ctgttcaaga tcggcgatat gcttgaagtc gatctggtca
     8641 aagtggatat ggcccgcaag ttcattgact tccagattgc cggacagaaa gaggcctcga
     8701 agttcatgcc gggcagaaaa aaagccggac ctccgcgcag taaacagaaa aaacagcagg
     8761 ttaccctgac cggcaaccgc ccgcacgggc cgaagcagca gaaacgacgt aaaaggaaat
     8821 agccgatgca ctccattaca aacgggattc tgaacgttta cgtcaagtcg cacggtgcag
     8881 aactctgcag tcttaaagac tccggcggta atgaacttct ctggcaggcc gatccggcgg
     8941 tctggaaccg ccacgctccg gtacttttcc cgatcgtcgg aaaactgctc gacggccgct
     9001 atacgctgaa cggcaaaacc tgcgaactgc cgccgcatgg atttgcacgc gacatggatt
     9061 tcgccctgat cgaacgacgc tcaagcgatc tcgtctatca acttctgccg acggcggaaa
     9121 cgcgcaagag ctatccgttc gaattcgact tccggattgc ctaccacctg accgggcaga
     9181 cgctgggcat caggtacacc gtgaaaaacg gaagcaaaga aaccatgccc ttctccgtcg
     9241 gtgcacaccc ggccttcatg ctgccggggc cgatcgacga atgctttctg gatttcgaaa
     9301 aaaacgaaac tctgtatgcc cgactgctca gcagcaaagg actgctttcg aatgaaacgg
     9361 tacccgttct gaaagattca aacagacttc cgctcagcaa aacactgttc gaccgcgatg
     9421 cgctgatttt tctggatacc gcatccgacc ggatcacgct gggcgcaaaa aacagctcgc
     9481 ggcgcctgac ggttgaattt cccggattcc cccagctcgg catctgggcg aagcccggcg
     9541 caccgttcgt ctgcattgag ccgtggtacg gtcatgccga tccggaacag ccgtatggtg
     9601 atatcatgaa taaaccgggc atccggcagc tgcccgcggg cggcacgttc acctgtgaac
     9661 accgtattct ggttaaggaa taagaccggc cggcttttcc aagcatcgga aaaatctgcg
     9721 tttttgatcg tcagcaataa cggcaaagcg tacaacccgc gccttatgca cattattttc
     9781 aaaaacggac tggccgccgc aaagaaaaac attggaccag gcctgctgct gcagggcttc
     9841 gcgctggcgc tgctgctgct gtattatttt catgcaccga cccgccagct gctgatgact
     9901 attcccgaaa tcaaagcgcg gatgggcatc agttttccgc tgctgatgac ggcattttcc
     9961 gggggaatcc tgccgttcat ttttctggcg ttccgcaaag agattcctgc cggacgttac
    10021 attcctcatc tgctgttcct gacgggattc tgggccttta acggtatgat ggttgatttg
    10081 ctgtatcaag gccaggccct gctgttcggc gatcaggccg atttcgccac gctgattaaa
    10141 aaaatcacag tcgatcagtt tgtgttctgc ccgctctatt ccgcgccggt tacagccgtc
    10201 gccatgcact ggaaaaattg cgacttttca tggcagaaga ccaaagcaga gctttcagcg
    10261 agagtgttcg cattcgaaat gctgtcggtc ctgatttcga tctggggtgt ctggctgccg
    10321 accgtcttca ttctttacag cctgccgctg gcgctgcagt ttccgctttt taatatcatc
    10381 ctctgcttct ggtcgctgct gctcagcgca ctgaacagta aaccgcagtc ggcataaatt
    10441 attcaggctg cgaagtgctg tcgtcgcaga aagctttgcg gacaatatcc tgcacaacca
    10501 gcccggcaag gttgaaaccg aaggctccgg tgatgtgaac catcgagccg ttaatctgcg
    10561 ctttctgatg gcaccagaca tccgacggaa tttcatttcc ttcatcgtcc agctgtttcg
    10621 ggcagaagca ctgcgccgaa ccgcaggcgg ctttcacagc ctcgaatgcc ggcagaaact
    10681 cctccgaata aacacacagg cagtcgccgg tcacgccgcg gctgcgcaac cgtttgcgga
    10741 cgaagtgggc cagtctgcag ccgcgcgatt tccagatgga atccacacgg atcgtcgtgg
    10801 catccagctt gctggcggca cccatcgcgg aaaacagcgt cacaccggat tccatcgcgg
    10861 aagcaatcag atcgaccttg tgcgacagac tgtcgatggc atcgataacg taatcataac
    10921 cggacagatt aaattccgcg cgcgtttcgt agttataaac tttctgcagc gccgtaatgt
    10981 ccgcgtcggg atggatatcc agcagccgct cctgcagtgc cagcaccttc ggctttccga
    11041 cgtttttcga cgtggcctga agctggcggt taatgttggt cacgcagatc acatcattat
    11101 cgacaatcgt cagatgaccg acgccggaac ggatcagcgc ttcggcgcac cagctgccga
    11161 cgccgcctac tccgaacaga atgacacggg tatccgccag ccgctgcagc gcatccgcgc
    11221 cgatcagcag ttcagtgcga tgaaatgccg gatgtttttt catgaaatac gtaacgcagt
    11281 tgaggaaata tcttcgcgga attttgattc aggaaccgct tcgaaaagag actcaaaccc
    11341 tgccggcagc tccatgtttt tcagcgtctg aaaagatccg ccgaactgac ggcccgcgac
    11401 aagaaagcgg gtttcaaggg tctggaactt tttccaatcg tccggcgaat agtccttcac
    11461 gaagcggatc gcagtatcga atccgacggc aaaggcggtt tttggaaaga gctgcgcttt
    11521 ctggataaag cgcggcgcat gcgtcagcgc aaccggaaca tctttaatgg cggcggcgcg
    11581 gcgcagtgca tcggcttctt caatcgccgg tttatcgaca ttcgcgcagg agagttcaaa
    11641 aacgccgcgc agaccggtca gttcctcggc ggctttaagc agattgcggt gtccctgatg
    11701 aaccggattg aacgaaccgg gaagcatcag tccgcgctcc gcgccaacgg cctgtgcaat
    11761 cagaacaagc agccagccgc ttaaaagcgc ctcctgctcc gcgcgggagg tttttgaaaa
    11821 atagagagcg tagagtcttt ccacgccagc cgttacaaaa ctgataaacg ccctatcttc
    11881 gccacgccgc tcatggtcgg tttgcagtgc agcagtacag ccgattccaa taggcaaaga
    11941 tgtgccaacg ccggaatgga atgctttttc ggcaagtttg ctggcagttg ccggagaaac
    12001 ggcttgttca ggtttctcgc ccagaaactc ttcaagtgct tcgagcgagt acggaatatg
    12061 cgctgctcga acaaaacgcg aagcgccggg cgtggtaagg atcgcattga gcgcacttga
    12121 accgccgccg gtggtgacca gcaccgcccg ccggccggag ttcaagatac gctctatgcg
    12181 ttcgctgctc acttaatcac agagacttcg gtgatggtga cggtttcgcg gggcacgtcg
    12241 cccggagcgg tcggaacagc ccgaatggca tctacaacgt ccatgccttc gacgactttg
    12301 ccgaaaacgc agtagcccca gccctgctga gtcggcgctt tgaaatcgag gaagccgttg
    12361 tcgacgacgt tgataaagaa ctgcgcgccg gcactgtgcg gatccgaggt gcgggccatc
    12421 gcgatggagc cgcggacgtt tttgagtccg ttgttggctt cgttttccac ggtgtacggc
    12481 gctttcttct gattcatatt ggctgtcatg ccgccgccct gaatcatgaa gttgctgatg
    12541 acgcggtgga aaatggtgtc cttataatgc ccgctttcaa catacttgag aaagttggcg
    12601 acggttttag gcgctttcgc cgcatcgagt tcgagtttga tatcaccttt ggtggttttc
    12661 atcagaatca tgactttggt ttcctttgct ggttccggag tattttcgac ttttgcttcc
    12721 acttcggccc gggaacagcc gacgagaaga aacgctgccg caaacacgcc gcacaatgcc
    12781 ttcagattgt ttttcatgtg caaaaaccta ttggtgttta ccgccaatgt ctataaagtt
    12841 cgttctcaat ttacggagga acccggctgt gaaaaatgaa gcttccgcca ttctgctgat
    12901 ctcctgccct gaccggcagg gcctggtgtg cgctgtcacc gatttcctga tgcgccataa
    12961 cggcaacatc atcgacctcg aacagcacgt tgacaccgaa gagcgcgtct ttttcatgcg
    13021 cgtcgagtgg gaactgaacg gcttcgccct gccccgcgac cagatcggcg aaaaattcag
    13081 cctgatcgca tcccgcttcg aaatgaaata cgagctgcac ttctcggact acaaaccgcg
    13141 cctcgccatc ttcgtttcga aatttccgca ctgcatgctc gacctgctgg cgcgccagag
    13201 ctccggcgac ctgaacgcgg aagtcccgct gattatcagc aaccataccg acatggaacc
    13261 cgttgcccgc atgcacggca tcgactttca ctgcataccg gtttcaaaag agaacaaagc
    13321 ggagcaggaa gcccggcaga tggaactgct caagcagtac agcattgatg tcattgtgct
    13381 ggcgcgctac atgcaggttc tctccgacag cttctgccgc aactacccca accgcatcat
    13441 caacatccac cactccttcc tgccggcctt cgtcggcgca aaaccgtacc actccgccca
    13501 ccagcgcggc gttaaaatcg tcggcgccac cagccactac gtcaccggcg atctggatga
    13561 aggtccgatc atcgagcagg aagtcatgcg cgtcacccac cgggactccg tcgaaagcct
    13621 gattcgcaaa ggccgggacc tcgaaaaact cgttctggcc cgcgcggtct gggaccacct
    13681 gcaaaacaaa gtgctggtct acaaaaaccg caccgtcgtc ttcggctgac catgacaaaa
    13741 gcccgccgac tccgccgccg cccgttagaa accgccgcct tccgcgccgg actggccgtc
    13801 gtcccccggc tgccgcgccg cgccctgctc ggccttgcct ggctcggcgg aaacctcgcc
    13861 tttctgtttg accgtcgcgg acggagggtc ggcctggcca acctcgacct cgccttcggc
    13921 gatgcaaaaa gcgccgccga aaaacgggcc attctccgcg cggccttcat caccatgacc
    13981 cgcacctttc tggacgtcat ctggttcggc acagactcaa agaaccggct gaaccgctac
    14041 gttgaactcg acagcagtgt tcagcagttc ctctgcccga aaaaccagat ctgcgtcacc
    14101 gcgcatttcg gcaactggga agtgatcggc caaatgatgg cgattcacgg attcccgctt
    14161 cacagcatcg ccatgccggt aaaaaacccc gatgtggatc gcctgctgat tgcccggcgc
    14221 gaagtgaccg ggcagaaaat tattccgcgc gacggcgcgc tgcgcaaact gctcggcgtc
    14281 ctgcgcaaca acggcaaaac cgccttcctg gtcgaccaga acaccaaaga aagcgaaggc
    14341 ggaatctggg cggattattt cggcctgccg gtgccggtca cacccgcccc ggcggcgctg
    14401 gcggtcaaga ccggcagtaa aattctgatc ggtttctgcg cgccgctgag cggcggacgc
    14461 taccgcgtct atgtcaccga aaccatccag ccgccggaaa atgcgggcga ggaaaccacc
    14521 cgcgaactga cccggcagat tctttccgcc atcgaacgcg aagtcagcaa acatcccgaa
    14581 cactggctct ggatgtacaa acgctggaaa aaaatatacc gc
//
LOCUS       DAOPZO010000035        13967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_4770_length_13967_cov_6.036084, whole genome shotgun sequence.
ACCESSION   DAOPZO010000035 DAOPZO010000000
VERSION     DAOPZO010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 13967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13967
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4770_length_13967_cov_6.036084"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1161)
                     /locus_tag="PKI68_03760"
     CDS             complement(52..1161)
                     /locus_tag="PKI68_03760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX52840.1"
                     /translation="MNNRRKLIVVGQTPPPFNGQAKMIRQMINGLCGEFDLLHIRMAY
                     SDSVVSAGKFGVAKILHLFRLICETRRALEENPGAVLYYPPASPNLLPVLRDIIFLLA
                     VRPLAGKTVFHFHSGGVSEFIKRHAWLAPAARAAYGSPDLAVELGASCPRDGTFFGAR
                     KVCLVPNGIDVPPPAGEKTDGEIGGIKILYVGIHTGSKGLFDLLETARELKRRNVVFE
                     ICTAGLWYTEKERRHFELLRAQWNLEAEVRTPGQKTGADLWALYEWADVFFFPTFYEW
                     ETFGLVQVEAMAYGLPVVASDWQGPKDVVLDGETGILCPVHNITAFADALQRLTQDRG
                     LRLKMGCAGQERYKRYFSADCFIRNIKRVLEEVCE"
     gene            complement(1154..2002)
                     /locus_tag="PKI68_03765"
     CDS             complement(1154..2002)
                     /locus_tag="PKI68_03765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX52841.1"
                     /translation="MKFSIITPSYNQLSYLRRCMASVADQSGVEVEHLVIDGGSTDGS
                     AGFLRESPAGRTAFLSEPDRGMYDAINKGLDRISGDTVAWLNCDEQYLPDALNKAARF
                     LAEHPSVDFVCGDVLMVGPDGRLLCCRKNPPLRRAYILADHLYVQSAAMFFRRSVFDS
                     GFRFSMEWKAVGDCDFVVRLLEAGFRAARIPDYLAACTMTGDNLSRQQSGVDELQLFR
                     RRAPALYRLGRPVWNLFRYSEKFLNGGYRQAVPFEYSLYLEDSGSRKKISMQQAEFRF
                     KWGPHE"
     gene            complement(1999..3885)
                     /locus_tag="PKI68_03770"
     CDS             complement(1999..3885)
                     /locus_tag="PKI68_03770"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNX52842.1"
                     /translation="MSRIEKTVIAVLTVALAAAFTFFGLWEDKRLLCAPLLVLIYLAL
                     SGWALSRFKNRTTSRLAVPPGGIPLLLFWFYGLALIPFSVLPYEAKVSTLYLGAYLSI
                     YWAVANLASRFSVRKAVWTSVFILLAFVALYSLVQHRTAPGFLFGVERYTDYWKGGRL
                     GGTYQCPNHIAHLFQMWLPFCLLFLFIPRFGWFWRICFAYALPLFLLLIYQTQSRAGL
                     LGAVAALGTAVLLMVLRKSRRAFCVALLAVPLLCAGLLGGLWAGSEMFRSRMQPVIQF
                     AQIIASGKNIDEHVTDFRPQTWLDTLEMINIRPVTGAGPGNYGQVFEDYRHRCQAFRV
                     ETVHSHNEYLELTAEYGLVGAALVLWIAVSLVFRMIRFIKTAERPYHALPAAALLGAL
                     AGTAVHGFFDFELHIFPNAVMLALLAGCAAAPLLQTEPAERSKWAAGLQRLFLAAVLC
                     ATVWSLQVMSSSWICAQGDRLFDSQSFQRAETFYQTARRIDPQNWQAHLGLGRVYFHY
                     RHFELDPARKHAWALKEQSACRNAYRINSKQDKVVYALGCAELFLGNRDEGLKYLRQV
                     AVYKRFNDFYWRKLGIELRKAGLYDEALAVFETARKLDGTNKTVKRNIEWLKARTGAA
                     GGAK"
     gene            complement(3938..>4822)
                     /locus_tag="PKI68_03775"
     CDS             complement(3938..>4822)
                     /locus_tag="PKI68_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNX52843.1"
                     /translation="MSDDYKRGEVAGDLPVLGNLHHTTRRMPVAVVALPDMGRHELIE
                     LLEGPLQTYNTILLVPDLQDAPSLWVTPCDIQGILGLKIRRNLLDPFPNFLKTFFERS
                     LVIVTLPVWGGLCLVFMLLVWLQDFNSSIYSQERIGKNGMRFKTYKLRTMVPDAEKVL
                     QRELASDPALKAEWEAHYKLKKDPRITPVGWFLRKTSLDELPQLWCVLIGTMALVGPR
                     PLPEYHHTELQPRTRRLRVKVRPGLTGLWQVSGRSDSGTSGMDKWDTYYVTNWSIWLD
                     IVIIAKTVRVVLFGSGAY"
     assembly_gap    4823..4922
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5133..7736)
                     /locus_tag="PKI68_03780"
     CDS             complement(5133..7736)
                     /locus_tag="PKI68_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNX52844.1"
                     /translation="MKTKLLNAFLSTLPLLLFTAILTGLLLIRIISGVALPLWSAWGL
                     GLAWCVMAFDYLLRAELFKQIGGFAVTLIAVLILIGALIPKETVARYPRMASAELVDT
                     FETTWNAVYTGNIFETFSPKQRLIDYGKSLASLDPFRHGKMILFGLLGFAVAVVYLFP
                     VSPLKRPKGSLKRPGGLVETVQGTSLKPSKTVKSEDTQNDLNESDKNGRFNDFNDLNA
                     KAVYLRRFLLLFSAGAVFAVQMELLQVLSPTRMVTGLSILDSCLGLLIGLLFFVPVHL
                     FYAFLAEHRAKGSSRFNVLGVGVDAVNMGDCLKLFERIIETVKPEQSSSLQPLNGEVE
                     KADFNEPSGRCNALNGLNESTARPVMTSALGVAGIVEARRNPKLQRILNESVLNTPDG
                     MPLVWLGKLFGYPDIERVYGPDLLRDVCAYSASRGWRHFFYGAAPGVVEKLKEKLEQK
                     HPQIQIAGIYCPPFRDLTPEEEAELVEKVNSSGTDIFWIGISTPKQLYFMDHIRPQLN
                     CKIICPVGYAFDVNAGVQEDAPDWAKYAGFQWLHRAIKQPRLWRRYLPDNPRFVVEVM
                     LQLLHLKKYPMFVHERPTEPFTDAEGYSRFPVGVVSLSAMTLQGACDRVINWVATGQR
                     HYVNICTADTMVQCFDQPQLAKIVNQAGMATTDGMPLVWLARYYGFSHATRVYGPDLM
                     LALCAATSTLQPQSEKKCVSHFFYGATEEVLAHLKSNLLKNFPGLQIAGMYSPPFRPL
                     TGTEKDDVAERINASGADIVWCGLGTPKQDYWVAEFRPRLRAAAILAVGAAFNFHAGE
                     IRQAPRWMMRSGLEWLFRLCVEPKRLWRRYLIGIPRFVGLISGSFFRRRYGLFSPGKI
                     KHLQARAGFSK"
     gene            complement(7778..8476)
                     /locus_tag="PKI68_03785"
     CDS             complement(7778..8476)
                     /locus_tag="PKI68_03785"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX52845.1"
                     /translation="MTSSVPRLPYRGLLVENTVACNLRCPGCIRPKVVKVRKKTQMSP
                     DDVRTAAELIRRLKIQKLFYFNRGEPFLSPHILDEIKIIRALNPDLYIVTSTNGMFLN
                     TDEKREAALLMDEVIFSIDGCDQKSLRKYQRGGDFNQMLKNLRELVCLRNERGLHVPQ
                     VEWKYLLFNWNDRPEQIRQAIQLANSTGADLISFWPTNDPAFGISWRYRFGLFGRIGT
                     KSWKGRELRLSGKI"
     gene            complement(8835..10040)
                     /locus_tag="PKI68_03790"
     CDS             complement(8835..10040)
                     /locus_tag="PKI68_03790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX52846.1"
                     /translation="MKIIQVHNRYLQPGGEDVVVAAERSMLQAHGHEVILFERSNLEI
                     DGWSPLQKIFRVIWNRQACVELEALIESFKPDVLHVHNVFHAISMSVYQAARRKGVPV
                     VQTLHNYRLFCLNGIFFRDGQVCEACLRYRFFPWAGVRYRCYRRSLSGSFLVAAMLAI
                     HRVCRTWISPVNRFIVLTDFCRDKFIQCGIPPEKIAVKPNFLSQDPGAGADVRNRQML
                     FLGRLTEEKGVRTLLKAWALFRERAAASAESGVSPELLIAGDGPLTAELSGDYGPGVK
                     MLGHIDRSSSVCFLKNVRALVFPSEWYETFGLSVVEAMACGTPVLVADANAAASLVRD
                     RKTGILFRSGDCGDLSEKIGWMMSHPEECAALGRAARQDYENRYTAEANYEKLIDIYR
                     QVARERQAC"
     gene            complement(10090..11379)
                     /locus_tag="PKI68_03795"
     CDS             complement(10090..11379)
                     /locus_tag="PKI68_03795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX52847.1"
                     /translation="MPTNTPSKTTNHQPAEKRRLRVLISAYACEPNKGSEPGVGWNIA
                     TEMAKLHDVWVLTRANNRTVIEDELARHPVPGLSFFYYDLPKWACWWKKGGRGVQLYY
                     YLWQMTAVSKVRKKHAEIRFDLCHHITFVKYWAPSCLAWLKIPFVWGPVGGGDSTPVR
                     FLRDAGLKGMIYEIVRDGIRHCAFLDPFVRFTAKRSSSCIAVTEKTAACVNRLNRSLS
                     PMLITQVGISERELQEIDQILPAEGKKTEITFLFVGNLLFLKGVHLGLKAFAESALSK
                     ARFLIVGDGPERRALESLAEELGISGRVHFLGQRSRNEVLSIMKSVDALIHPSLHDSG
                     GFVLVEAMACRKPVLCLDLAGPALLVPENAGIRVQADTPVQVSRDIAAGMQRWVANPE
                     LRMRMGDAGREMIESQLLWSKKTASFSDLYAAVLKDF"
     gene            complement(11432..12370)
                     /locus_tag="PKI68_03800"
     CDS             complement(11432..12370)
                     /locus_tag="PKI68_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNX52848.1"
                     /translation="MATLDFILKDPSILFRAIRRKGELRYVASNRPGSLAAATRFAPP
                     TKPLPKLFLRLGALVDFRLPGFAEPIRISSEFLEQAADRLQIPADRLGMYAGEPNPQQ
                     KLVVADVERKSPQILKLALGQEADAMIARELEALLAVDGLRIGTSVIRSPKVQHAAPV
                     AGRSAMLIERIDGRQFSPKEFEAIFLNFDCTRGRGASLVRVGEWLDQTAVQQTQRLAP
                     LLAACRAADALDLLSARGRVQGDFMSWNLMKTSADPVLIDWEFSYPDVPVIFDKAYAA
                     YCYAELLGLRLKGLSDIEWTKLVALGALWKELRQQL"
     gene            complement(12427..12783)
                     /locus_tag="PKI68_03805"
     CDS             complement(12427..12783)
                     /locus_tag="PKI68_03805"
                     /inference="COORDINATES: protein motif:HMM:NF008912.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNX52849.1"
                     /translation="MAYQSFEDLEVWKLSLDLSVRLYERLGGCKDFGFKDQICRAAVS
                     VPSNIAEGIERNSKKETIHFLHIAKGSCAEVRTQLIIARRIGYIDYANAEKMTADAVS
                     VSRMLHGLIKALGDAH"
     gene            complement(12796..13440)
                     /locus_tag="PKI68_03810"
     CDS             complement(12796..13440)
                     /locus_tag="PKI68_03810"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52850.1"
                     /translation="MSCSGICIVFFGPDGCGKTSVAEGVKRALAGTFPLERGLHCHWK
                     PIRPKGSGVAPTVDPHRVPPRSRMLSLAYFVYHYLPFIWGWWGHVNPALRKGGLVMID
                     RYYYDFFVDLRRYRLNLPRWVVNLGFVFIKKPELVFCLDADPEILQARKKEVSFEECK
                     RQREAYRALAEKLPNGHVIDASQPLEKVVRDVQSVVLNYLAERTRCRSLHRVKF"
     gene            complement(13437..>13967)
                     /locus_tag="PKI68_03815"
     CDS             complement(13437..>13967)
                     /locus_tag="PKI68_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52851.1"
                     /translation="EAAGLIAAIDRKDLQWINVHLMALRCVLLSRALLRPVELVQALF
                     AWVRNEWVESIMPYPITPIVLLTGNKELIDYAAQTLSKRFEERFVFTSVKIQEGAKGH
                     SIRFLWKRRVRSSRLGLTLVLYDVALPTQASLFRLISPVNLVLNVKSTDPDSILGYER
                     QIIQCFAKMNASKDQF"
BASE COUNT         3518 a         4116 c         3424 g         2809 t
ORIGIN      
        1 caatcaggtg ccgtccgaca aatccgcatc cgcctgttac cagtgatctt ttcattcaca
       61 aacctcttca agaacccgtt tgatgttccg gataaagcaa tctgctgaaa aataccgttt
      121 atatcgttcc tgtcctgcgc agcccatttt cagcctcagc ccgcgatcct gagtcagccg
      181 ctgaagtgcg tcggcaaatg cagtaatgtt gtgcaccggg cagagaatac cggtctcccc
      241 gtccagcacc acatctttcg gtccctgcca gtcggaggct accaccggca gcccgtacgc
      301 catcgcttcc acctgcacaa ggccgaaggt ttcccattca taaaaagtcg ggaagaaaaa
      361 tacatccgcc cattcgtaaa gagcccaaag atccgcgccg gttttctgtc ccggcgtccg
      421 gacctcggcc tcgaggttcc actgcgcacg gagcaattcg aaatgccggc gttctttttc
      481 cgtataccac agaccggcgg tgcagatttc aaaaaccacg ttccggcgtt tgagctcgcg
      541 cgcggtctcc agcagatcaa agagtccttt tgagccggta tgaattccga catagagaat
      601 ttttattccg ccgatctctc cgtccgtttt ttcaccggca ggcggcggaa catcaatacc
      661 gttcggcacc agacaaactt ttcgcgcgcc aaaaaacgtt ccgtcacgcg ggcaggaggc
      721 tccgagttcg accgccaaat ccggcgagcc ataggctgcc cgcgcagccg gcgcaagcca
      781 cgcgtgccgt tttataaact ccgaaacccc gccggagtga aaatgaaaaa ctgtttttcc
      841 agccaaaggc cgaaccgcca gcagaaaaat aatatcccgc agaacgggca gcagatttgg
      901 agatgcgggc ggataataaa gaacagcgcc cggattttct tccagcgccc ggcgggtttc
      961 gcaaatcagc cggaacagat gaaggatctt tgcaaccccg aacttgccgg cggaaacaac
     1021 cgaatcactg taggccatcc gaatgtgaag cagatcaaac tctccgcaaa gtccgttgat
     1081 catctgacga atcatcttcg cctgaccgtt gaacggcggc ggagtctgtc cgacgacgat
     1141 aagcttgcgg cggttattca tgcggccccc atttgaagcg gaactccgct tgctgcatgg
     1201 aaattttttt acgggagccg gaatcctcca gataaagact gtactcgaac ggcacggcct
     1261 gccggtaacc gccgtttaaa aatttttccg aatagcgaaa cagattccaa accgggcgcc
     1321 ccagccggta taacgccgga gcccgccgac ggaagagctg caattcatcg acgcccgatt
     1381 gctgacggct caggttatcg ccggtcatcg tgcaggccgc cagataatcc ggaatccggg
     1441 ccgcccggaa cccggcctcc agcagccgca caacgaaatc gcaatcgccg accgccttcc
     1501 attccatgct gaacctgaat ccggaatcaa aaaccgaacg ccggaaaaac atggcggcgc
     1561 tttggacata aagatggtcg gccagtatat atgcccggcg cagcggcgga tttttccggc
     1621 agcacaaaag ccgtccgtcc ggaccgacca tcagcacatc gccgcaaaca aaatcaaccg
     1681 acgggtgttc ggcgagaaaa cgggcggctt tattcagcgc atccggcaga tactgctcat
     1741 cacagttaag ccaggcgacc gtgtcgccgg atatccggtc gagcccctta ttgatcgcat
     1801 catacatccc ccggtcgggc tcgctgagga aagcggtccg cccagccggg gactcccgca
     1861 gaaacccggc ggagccgtca gtggaaccgc catcgatcac caggtgttcg acttcgactc
     1921 cggactgatc agcgactgaa gccatacacc gccggagata cgacagctga ttgtacgacg
     1981 gagtaatgat cgagaatttc atttcgcacc gccggcggcg ccggttcttg ctttgagcca
     2041 ctcaatattt cttttcaccg tcttgtttgt gccgtccagc ttccgggcag tctcaaagac
     2101 cgcaagcgcc tcgtcataaa gcccggcctt gcgcagttcg atgccgagct tgcgccagta
     2161 aaaatcgttg aagcgtttat agaccgcaac ctggcgaaga tacttcagcc cctcatcccg
     2221 gtttcccaga aacagctccg cgcagccgag tgcatacacc actttgtcct gcttggaatt
     2281 aatacggtat gcgttccggc aggcagactg ttccttgagc gcccatgcgt gtttacgggc
     2341 cggatcgagt tcgaagtgcc ggtaatggaa atagactctc ccgagcccga gatgcgcctg
     2401 ccagttctgc ggatcgatcc ggcgcgcggt ctgataaaat gtttctgcgc gttgaaaact
     2461 ctggctgtcg aacaaccggt caccctgtgc acagatccag gacgaactca taacctgcag
     2521 ggaccagacc gttgcgcaga gcacggcggc aagaaacaac cgctgcaggc cggcagccca
     2581 tttcgaacgt tccgccggct ccgtctgaag cagcggagcc gccgcgcatc cggccagcag
     2641 cgccagcatc acggcgttcg gaaagatgtg cagctcgaaa tcgaaaaatc cgtggaccgc
     2701 cgtacccgcc agcgcgccga gaagcgcggc cgccggcaga gcgtgatacg ggcgctctgc
     2761 ggtcttgatg aagcggatca tccggaagac gaggctgaca gcaatccaca aaaccagcgc
     2821 cgcgccaacc agaccgtatt ccgcggtcag ctcgagatat tcattgtgcg aatgaaccgt
     2881 ctccaccctg aaggcctggc accgatggcg gtaatcctca aacacctggc cgtagttgcc
     2941 ggggccggct cctgtcacag gacggatatt gatcatttcc agcgtgtcga gccaggtctg
     3001 cgggcggaaa tcagtaacat gctcgtcgat gttcttgccg gaggcaatga tctgcgcgaa
     3061 ttgaataacc ggctgcatac gggagcggaa catttccgat ccggcccaga ggccgccgag
     3121 cagtccggcg caaagcagcg gcacggccag cagtgcgaca caaaaagcgc ggcggctttt
     3181 acgcagcacc atcagcagga cggccgtgcc taacgcggcg actgcgccga gaagaccggc
     3241 ccgcgattgt gtctggtaga tcagcagcag aaacagcggg agtgcatagg cgaagcagat
     3301 gcgccagaac cagccgaacc ggggaataaa caaaaacagc agacagaaag gcagccacat
     3361 ctgaaagagg tgcgcgatgt gattcgggca ctgataggta ccgccgagcc gtccgccttt
     3421 ccaataatcg gtatagcgtt ccacgccaaa cagaaagccg ggagccgtgc gatgctgcac
     3481 cagactgtag agagcaacaa atgccagcag aataaaaacg gaggtccaca cggctttgcg
     3541 cacagaaaac cgggatgcca gattggcaac ggcccagtaa atcgacagat aggctccaag
     3601 atacagagtg ctgacctttg cctcgtacgg cagaacagaa aacggaatca gagccagacc
     3661 gtagaaccag aaaagaagca gaggaatccc tccgggagga accgccagcc gggaagttgt
     3721 acggttttta aaccggctca gcgcccagcc ggaaagtgcc agataaatca gcacaagcaa
     3781 cggagcgcaa agcagacgtt tatcttccca taaaccgaaa aatgtaaaag ctgccgccag
     3841 tgcaaccgtc agaaccgcaa tcactgtttt ttcgatacgg gacatacacc attccgcaaa
     3901 acgccgacct gcctgcgtgc gaactcgcca aaactcttta ataagcgccg ctgccgaaca
     3961 gcaccacacg caccgttttg gcaataatca caatatccag ccagatggac cagttggtga
     4021 cataataggt atcccactta tccatgccgg aggttccgct gtcactccgt cccgaaacct
     4081 gccacagacc ggtcagtccc gggcggacct tgacgcgcaa ccgccgtgtg cggggctgta
     4141 attccgtgtg gtgatattcg ggaagcggcc gcggtccgac cagcgccatg gtgccaatca
     4201 gcacgcacca aagttgcggc agttcatcca gagaggtttt ccgcagaaac cagccgaccg
     4261 gtgtaatgcg agggtctttt ttcagcttat aatgcgcctc ccattcggcc ttcagtgccg
     4321 gatccgaagc cagctcccgc tgaagaactt tttccgcatc aggaaccatt gtccgcaatt
     4381 tgtaggtttt aaaccgcatg ccgtttttac cgatccgttc ctgagagtaa atcgacgagt
     4441 taaaatcctg aagccagacc agcagcatga aaacaagaca aagccccccc cacaccggca
     4501 aggtcacaat caccagtgaa cgttcgaaaa acgtcttcag aaaattggga aacgggtcga
     4561 gcagattgcg tctgattttg aggccaagaa ttccctggat atcgcacgga gtaacccaca
     4621 acgatggcgc gtcttgcagg tcgggaacca gtaaaatggt attgtaagtc tgaagcggcc
     4681 cttcaagcag ctcaatcagc tcatgccgtc ccatgtccgg aagcgcaacc acagcgacgg
     4741 gcatacgccg ggttgtatga tgaagattgc ccagcaccgg caaatccccc gccacttcgc
     4801 cccgcttata atcatcactc atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnccgttacc ggccagcaga gccagagaca gcatcaatcc gtcgcttaaa ctcaggatta
     4981 tggagtctgc catccgtcgc cggagaaaag gatgtgcagt ctgaaagtca gaatcaacag
     5041 tggttcctgt gttaaaagat ctcatacgca gttaaatcat gaattgaggt ttgccgcagt
     5101 gtctcgcaaa aagccgtaaa agtcacagga aattacttgc tgaatcccgc ccgcgcctgg
     5161 agatgtttta ttttcccagg tgagaacaga ccgtatcttc ggcggaagaa agatccggaa
     5221 ataagaccga cgaaccttgg aataccgatg agataccgtc gccacaacct tttcggctcc
     5281 acacacagac ggaacagcca ttccagcccg ctgcgcatca tccatctcgg ggcctgccgg
     5341 atttcccctg catggaaatt aaatgccgca ccaacagcca gaatcgccgc ggctctcagg
     5401 cgggggcgga attcggccac ccagtaatcc tgttttggcg tgcccagtcc gcaccagaca
     5461 atatccgcac cggaggcatt gattctttct gccacatcat ctttttcggt tccggtcaga
     5521 ggacgaaacg gcggcgaata catacccgca atctggagcc cgggaaaatt cttcagtaaa
     5581 ttggacttca aatgagcaag gacttcttcg gtggcaccgt aaaagaagtg cgatacacat
     5641 tttttttcag attggggctg aagagtggac gttgccgcac acagtgccag catgaggtcc
     5701 ggcccataaa ctcgcgttgc atgactgaac ccgtaatagc gggcaagcca gacgagaggc
     5761 atcccgtccg tagtcgccat cccggcctga ttaacgatct tcgccaattg aggctgatcg
     5821 aaacactgca ccatcgtatc tgcggtgcag atattcacat agtgcctctg tcctgtcgcc
     5881 acccagttta tcacacggtc acaagctcct tgcagagtca ttgcggaaag agaaacgacc
     5941 ccgacaggaa accgggaata cccctcggca tctgtaaacg gttcggtcgg ccgttcatga
     6001 acaaacatcg ggtatttttt caaatgcagc agttgcagca taacttcaac gacaaaccgg
     6061 ggattgtcag gcagataacg cctccagagc cgcggctgtt taattgccct gtgcagccac
     6121 tggaaccccg catatttcgc ccagtccgga gcgtcctcct gaactccggc attcacatca
     6181 aaagcgtagc cgacgggaca aatgatctta cagttcaact gcggccggat gtgatccata
     6241 aaatagagct gcttcggcgt ggaaattccg atccaaaaaa tatccgtacc ggagctgttc
     6301 accttttcaa cgagctcagc ttcctcttca ggcgttaagt cgcgaaacgg cgggcagtaa
     6361 atgccggcga tttgaatctg aggatgtttc tgttccagct tttctttcag cttttcgaca
     6421 actcccggag ccgcgccgta aaagaaatgc cgccagcctc tggatgcgga ataagcacaa
     6481 acatcacgca gcagatcggg cccgtaaacc cgctcaatat ccggatagcc gaacagtttg
     6541 ccgagccaga ccagcggcat gccatccggc gtgttcagca ccgactcgtt caaaatgcgc
     6601 tgcaacttcg gattgcgccg ggcttcaaca ataccggcga cgcccagagc gcttgtcatg
     6661 acgggacgag ctgtgctctc gttcaaaccg ttcaaggcat tgcaacgccc ggagggctcg
     6721 ttaaaatcgg ccttctcgac ttcaccgttt aacggctgca acgaggaact ctgttccggt
     6781 ttaacggtct ctatgatcct ctcaaataat ttcagacaat cgcccatatt caccgcatcg
     6841 actccgacac cgagtacgtt gaaccgagaa gatcctttgg cgcggtgttc agccagaaaa
     6901 gcatagaaca gatgcacagg aacgaagaat aacaatccga tcagcagacc cagacaactg
     6961 tccagaatac tgagacctgt caccatgcgg gtcggagaca acacctgaag cagctccatc
     7021 tgaaccgcaa aaacagcacc ggcgctgaat agcaggagaa agcggcgaag atacaccgct
     7081 ttggcgttca agtcgttaaa atcgttaaac cgtccgtttt tatcggactc gttcaaatcg
     7141 ttctgagtat cctccgactt taccgttttc gacggcttta acgaggtgcc ctgcaccgtt
     7201 tcaacgaggc cgccaggccg tttcaacgag cccttcgggc gtttcaacgg actgaccggg
     7261 aagagatata ccaccgccac ggcaaatcca agcagaccga aaaggatcat ctttccatga
     7321 cgaaacgggt caagactcgc aagagactta ccataatcaa tcaaccgctg cttcggactg
     7381 aatgtttcaa atatattgcc tgtatacacc gcattccagg tggtttcaaa tgtatcaacc
     7441 aattcagccg aggccatacg cggatagcgg gcaaccgtct cctttgggat caacgcgcca
     7501 atcaaaatca gcacggcaat cagtgttaca gcaaacccgc cgatctgctt aaacagttct
     7561 gcgcgcaaca gataatcgaa ggccatcaca caccaggcca gaccaagccc ccacgcgctc
     7621 cacagcggga gcgccactcc ggaaatgatc cggatcagaa gaagcccggt taaaatcgcc
     7681 gtgaacagaa gcagcggcag agtgcttaga aatgcattca gcagttttgt tttcatagat
     7741 acggtctata tgatttgcac ggagctgacg gccagcatca aatcttaccg gacaaccgga
     7801 gctcccgccc tttccagctt tttgttccga ttcgaccgaa aagcccgaag cgataccgcc
     7861 acgagatgcc gaaagccgga tcatttgtcg gccagaaaga aatgaggtcg gcgcccgtgc
     7921 tattcgccag ctgaatcgcc tgccggatct gttcaggccg gtcattccag ttaaacagca
     7981 gatatttcca ttcgacctgc ggaacgtgca gcccgcgttc attgcgtaaa caaaccagtt
     8041 cccgaagatt tttcagcatc tgattaaaat cgccgccgcg ctgatacttc cgaagggatt
     8101 tctgatcgca gccgtcgatt gaaaaaatca cctcatccat cagcagggcc gcctcgcgtt
     8161 tttcatcggt gttgagaaac atcccgttgg ttgaagtgac aatgtacaga tccgggttca
     8221 gcgcccggat aatttttatc tcatcgagaa tgtgcggaga aagaaacggt tccccgcgat
     8281 tgaaataaaa cagtttttga attttgaggc gccggatcag ctccgctgct gtgcggacat
     8341 catccggcga catttgagtc ttcttacgaa cctttaccac cttcggacgg atgcagccgg
     8401 gacagcgcag attgcaagca accgtgtttt caaccagcaa accgcggtaa ggcaggcgcg
     8461 gcactgaaga agtcactcct ctttgatgtc tttcagaccg aatccgccgt aattctgaac
     8521 acctcgcaca cgtcagaatc ggcagctttc ctccggccag cgacgcgcgg aaggaatttg
     8581 ccacgggacc gaagaaaatc tcctcaaaac tgttttgatc caaagacccg agaatccctg
     8641 acccatcatc ctggcagttg caggaaacgg tcatgtcgca attaattgaa atgttgtatt
     8701 cggattcccc ctgcagggct gcacacgaaa atccacgccc tgaaatgaag gcctgaatag
     8761 tgtaaaaaat ccgaaaaaaa caacgaacca aggtgctcat tccaactcca gccgccagca
     8821 gacgtcccaa ggcattaaca ggcctgccgt tcacgggcaa cctgccggta gatatctatc
     8881 aacttttcgt aattggcctc ggcggtatac cgattttcgt aatcctgccg ggccgctctc
     8941 cccagcgcag cgcactcttc cggatggctc atcatccatc cgattttttc ggacagatct
     9001 ccgcagtcgc cggaccggaa cagaatcccc gtcttccggt cgcgcacaag agaggccgcg
     9061 gcattggcat ccgcaaccag gaccggcgtg ccgcacgcca tcgcctcaac aacagacaac
     9121 ccgaacgtct cgtaccactc cgacgggaaa accagcgccc tgacattttt cagaaaacaa
     9181 acggatgaac tccggtcaat atgcccgagc atcttcacac ccggaccgta atccccggaa
     9241 agctctgcgg tcaacggacc gtcgccggca attaataatt ccggactcac cccggactct
     9301 gcgctcgctg cagccctttc cctgaaaagc gcccaggcct tcagcaaggt tcgaacgccc
     9361 ttttcttcgg ttagccgtcc gagaaacagc atctgcctgt tgcgaacatc cgcgcccgcc
     9421 cccggatcct gcgaaagaaa attaggttta accgctattt tttccggcgg aattccgcac
     9481 tggatgaatt tatcgcggca aaaatcagtc agcacaataa agcggttcac cggcgaaatc
     9541 caggtgcggc acacccgatg aatcgcaagc atggccgcga ccaggaagga gcctgacagc
     9601 gaccggcggt aacacctgta ccgcactccg gcccatggaa agaaccggta ccgcaagcag
     9661 gcctcacaca cctgtccatc ccggaaaaaa atcccgttca ggcagaaaag acggtaatta
     9721 tgaagcgtct gcacaacggg aacccctttg cggcgagccg cctgataaac cgacatggag
     9781 attgcatgaa agacgttatg aacatgcagt acatccggct taaaagactc gattagggct
     9841 tcaagctcga cacaagcctg acggttccag atcacacgaa agattttctg cagcggcgac
     9901 cagccatcaa tctccagatt cgatctttca aaaagaatca cttcgtggcc gtgcgcctgc
     9961 aacatgctgc gctccgccgc gaccacaaca tcttcgccgc ccggctgtaa ataacggtta
    10021 tgaacctgga taattttcat gagaacccat cacatatgtt tctgccgaaa atcgaagctg
    10081 cgcactccgt taaaaatcct ttagaaccgc cgcataaaga tcagaaaagc tcgcggtttt
    10141 tttactccaa agaagctggg actcgatcat ctcccgccct gcgtcaccca tccgcatccg
    10201 taattccgga tttgccaccc acctttgcat tccggcggca atgtcccgac ttacctgaac
    10261 cggggtatcc gcttggacgc gaatgcctgc attttcaggc accaatagcg caggtcccgc
    10321 cagatccaga cacaaaacag gttttcgaca ggccatcgct tcaaccagaa caaagccgcc
    10381 ggaatcatgc aggctgggat gaatcagggc atccaccgac ttcataatcg acaacacttc
    10441 attcctcgag cgctgcccca gaaaatgaac tcgccccgaa attccgagtt cttccgccaa
    10501 gctctccaga gctctccgct caggcccatc gcccacgatc aaaaaccggg ccttgctcag
    10561 cgcgctttcg gcaaaggcct tcaatccgag atgaacgcct tttaaaaaaa gcagattacc
    10621 gacaaaaaga aacgttatct cggttttttt gccttccgca gggagaatct gatcaatctc
    10681 ctgtagttct ctttcggata ttccgacctg cgtaatcaac atcggactca acgagcggtt
    10741 cagtctgttt acacacgcgg ctgttttttc tgtaacagca atacatgaac tgcttctttt
    10801 cgccgtgaat cgaacaaatg gatctagaaa agcacagtgc cggattccat cacgcacaat
    10861 ctcatagatc attcctttca agccggcatc gcgcaggaaa cgcaccggcg tagaatcccc
    10921 gcccccgacc ggcccccaaa caaaaggtat tttcaaccat gcgagacagc tcggagccca
    10981 gtattttaca aacgtaatat ggtggcaaag atcgaaccgg atctccgcat gttttttacg
    11041 aactttggaa acggctgtca tctgccacaa ataataatag agctgcacgc ctcgcccgcc
    11101 ttttttccac cagcaggccc acttcggcag atcgtaatag aaaaagctca atcccggcac
    11161 cggatgcctg gccagctcgt cctcgatcac cgtacgattg ttggcgcggg tcagcaccca
    11221 gacatcgtgc agcttcgcca tctccgttgc gatattccac ccgacgcccg gctcggagcc
    11281 tttgttgggc tcgcaggcat acgccgagat caaaacccgc aaccgacgtt tttctgccgg
    11341 ctggtgattg gttgtttttg atggggtgtt ggttggcata aaaaccgcgt gttaggtgtt
    11401 atgttttagg cttatagcta acggctgatg gctagagctg ctggcgcaac tctttccata
    11461 aggcacccag cgccacaagt ttggtccatt cgatgtcgct caagcccttc agccgcaggc
    11521 ccaacagttc cgcataacaa tacgctgcgt aggctttatc aaaaatcacc ggaacgtcgg
    11581 gatacgagaa ctcccagtcg atcaaaacag gatcggcgga ggttttcatc aaattccagg
    11641 acataaaatc tccctgcaca cgtccgcgcg ccgacaacag gtctaaggcg tctgccgcac
    11701 gacaggctgc aagaagcggc gccaggcgct gggtctgttg aacggcggtc tgatcaagcc
    11761 actctccaac acgcaccagg ctcgcgccac ggccccgtgt gcagtcgaag tttaagaata
    11821 tcgcttcaaa ttccttggga gagaactggc gtccgtcaat ccgttcaatc agcatcgctg
    11881 atcgtccagc gaccggggcc gcgtgctgaa ccttgggcga gcggatgacc gaagtcccga
    11941 tgcgcagccc gtccaccgcc agaagcgctt caagttcgcg agcgatcatc gcatcagcct
    12001 cttggcccaa agccagcttt agaatctgcg ggcttttccg ctcaacatcc gcaaccacca
    12061 atttctgttg ggggttcggt tcgccggcat acatacccag ccggtccgcc ggaatctgca
    12121 aacggtcagc ggcctgttcc agaaattcgc tggaaatcct gatcggctcc gcgaagccgg
    12181 gcaaccggaa atcgaccagc gcgcccagtc ggagaaacag cttcggcagc ggtttggtcg
    12241 gcggcgcaaa gcgggttgcg gccgccagac ttccgggccg gttcgacgcg acatagcgca
    12301 gctcgccctt gcgccggatg gcgcggaaga gaatggaggg gtcctttaaa atgaagtcga
    12361 gagttgccat ccggcactgt gttaggtttt aggtttaaag cttcaggaat ccgtacccgt
    12421 tacatttcaa tgcgcatcgc cgagtgcctt gatgaggccg tgcaacatac gggataccga
    12481 caccgcatcg gccgtcatct tttcagcgtt tgcataatca atgtagccaa tgcgcctggc
    12541 aatgatgagc tgcgtgcgaa cttctgcaca ggatcctttt gctatatgca ggaagtggat
    12601 tgtttctttc ttggaattcc gctcgattcc ctcggcaata ttcgatggaa ccgaaacggc
    12661 tgcccggcaa atttgatctt taaacccgaa atctttgcac ccaccaagcc gctcatacag
    12721 acgcacgctt aaatccagag acaacttcca aacttccaga tcctcaaagg actgataggc
    12781 cataacacct gccccttaaa acttaacacg atgaagactt ctgcagcggg tgcgttccgc
    12841 caggtagttc agaacgacgc tctgtacatc ccgcacaacc ttctccagcg gctgggaggc
    12901 gtcaatcaca tgcccgttcg ggagtttttc agccagagcc cggtacgcct cgcgctgccg
    12961 tttgcactcc tcaaacgaaa cctctttttt gcgcgcctgc agaatttccg gatcggcatc
    13021 caaacagaac acgagctccg gctttttaat gaaaacaaag ccgagattca cgacccagcg
    13081 gggcagattg agccggtagc ggcgcaaatc gacgaagaag tcgtagtaat agcggtcgat
    13141 catcaccagc ccgcccttgc gcaaggccgg attcacatgc ccccaccagc cccagataaa
    13201 cggcagataa tggtagacga aataggccag actcaacatc cggctgcgcg gcggcacacg
    13261 atgaggatcc accgttggag caacgcccga ccccttgggg cgaatcggct tccagtgaca
    13321 atgcaacccg cgttcgagcg ggaaagttcc cgccaaggcc cgcttcaccc cttcggccac
    13381 gctggttttc ccgcagccgt ccggtccgaa aaaaacaatg catattccac tgcagctcaa
    13441 aactggtcct tcgatgcatt cattttcgca aagcactgta taatctgtct ttcataacca
    13501 aggattgaat cgggatcggt ggacttaaca ttcagaacaa gattgaccgg acttatcagc
    13561 ctaaataagc tagcctgagt cggtagcgcc acatcataca acaccaacgt aagcccgagg
    13621 cggctgcttc gcacacgacg tttccacaga aaccgaatac tgtgcccttt cgcaccctcc
    13681 tgaatcttca cgcttgtgaa aacaaagcgt tcttcaaatc tttttgagag agtctgtgcc
    13741 gcatagtcaa ttagctcctt attaccagtc aataaaacga taggggtaat tggatagggc
    13801 ataatcgact ctacccactc attcctgacc caggcaaaca aagcttgcac taactcgacg
    13861 ggcctcagaa gagcacgaga aagcaacaca catcgaagcg ccattaaatg aacattaatc
    13921 cactgcaaat cttttctatc gatagccgca attaacccag ccgcttc
//
LOCUS       DAOPZO010000036        13941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_4786_length_13941_cov_8.272649, whole genome shotgun sequence.
ACCESSION   DAOPZO010000036 DAOPZO010000000
VERSION     DAOPZO010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 13941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13941
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4786_length_13941_cov_8.272649"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PKI68_03820"
     CDS             complement(<1..512)
                     /locus_tag="PKI68_03820"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNX52852.1"
                     /translation="MLKIGQTVPDFQMDVFHNEEIRKIKLSDYKGKWVVLIFYPADFT
                     FVCPTELKEAALLYPKFQKAGAEIISISTDTAFVHKAWHDESEAIKTVKYPMGADPTG
                     NICRAFGTYIESEGLSLRGTFIIDPDGVLKTVEMHDLGIGRSAVEALRKLEAAKFVRE
                     HGDQVCPAAW"
     gene            859..934
                     /locus_tag="PKI68_03825"
     tRNA            859..934
                     /locus_tag="PKI68_03825"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:892..894,aa:Thr,seq:ggt)
     gene            994..2196
                     /gene="tuf"
                     /locus_tag="PKI68_03830"
     CDS             994..2196
                     /gene="tuf"
                     /locus_tag="PKI68_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008426562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNX52853.1"
                     /translation="MAKDKFERTKPHVNVGTIGHVDHGKTTLTASITCVQGAKGLANY
                     IAYDQVAKASESQGRRDPTKILTIATSHVEYSTANRHYAHVDCPGHADYVKNMITGAA
                     QMDGAILVVSACDGPMPQTREHILLARQVGVPAIVVFMNKCDLVDDTELLDLVELEIR
                     ELLSKYEFPGDDTPVIRGSATGAIKDPTGPGAACIQELMDALDSYIKEPVRVTDQAFL
                     LPIEDVFSIEGRGTVVTGRVERGVIHPGDDVEIVGLKPTAKTTCTGVEMFRKLLDEGR
                     AGDNVGCLLRSTKKEDVERGQVLAKPGSILPHTQFKSEVYVLSKEEGGRHTPFFKGYR
                     PQFYFRTTDVTGDITLPEGVEMVMPGDNVAMEVTLITPIAMEQHMRFAIREGGRTVGA
                     GRVTEVLK"
     gene            2283..2432
                     /gene="rpmG"
                     /locus_tag="PKI68_03835"
     CDS             2283..2432
                     /gene="rpmG"
                     /locus_tag="PKI68_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNX52854.1"
                     /translation="MRDIITLACTECKRRNYTTTKNKKLSTGRLEVKKFCPHDRSHTV
                     HKETK"
     gene            2451..2526
                     /locus_tag="PKI68_03840"
     tRNA            2451..2526
                     /locus_tag="PKI68_03840"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2484..2486,aa:Trp,seq:cca)
     gene            2546..2749
                     /gene="secE"
                     /locus_tag="PKI68_03845"
     CDS             2546..2749
                     /gene="secE"
                     /locus_tag="PKI68_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNX52855.1"
                     /translation="MKKIIELFKALKTFLSEVKIELKKCTWPTRQELFGSTMVVVISV
                     VILGVFVGLSDTVLMGLLRAVLR"
     gene            2768..3316
                     /gene="nusG"
                     /locus_tag="PKI68_03850"
     CDS             2768..3316
                     /gene="nusG"
                     /locus_tag="PKI68_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNX52856.1"
                     /translation="MEKHWFVLHTLSGHEYKVQNSIVSRVKQEEMGEYIGEVLIPTEK
                     VSEVKQGKKTTVSRKFFPGYVLANLALFDEKKNLIEETWYFIQNTPGVIGFVGGEAPV
                     PLRADEVDRIINQVEEKKETVTPKINFEPGETIKITDGPFLNFNGVIEEVDPGRGKLK
                     VSVSIFGRSAPVELEYWQADKA"
     gene            3335..3757
                     /gene="rplK"
                     /locus_tag="PKI68_03855"
     CDS             3335..3757
                     /gene="rplK"
                     /locus_tag="PKI68_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNX52857.1"
                     /translation="MAKKEKGMIKLQIPAGQANPAPPVGPALGQHGVNIMEFCKAFNA
                     ATQKEAGMIIPVVITVYQDRSFSFITKSPPASALLKKAAAVAKGSGVPNRDKVGKVTL
                     AQVEEIAKIKEADLNASDRAAACRIIAGTARSMGIEVE"
     gene            3760..4458
                     /gene="rplA"
                     /locus_tag="PKI68_03860"
     CDS             3760..4458
                     /gene="rplA"
                     /locus_tag="PKI68_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003637773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNX52858.1"
                     /translation="MATHGKKYTAVKKNVSVEKLYTVEEAIQFIKDNPTAKFDETIEV
                     ALRMGVDPTKSDQAIRSTVALPNGTGKDVRVIVFASGAAAEAARAAGAVEAGFEDLLA
                     KVTGGWMDFDIVVSTPDAMKEVRKLGKVLGPRGLMPNPKTGTVTDDTATAVKQFKAGR
                     VEFRMDKNGNVIVPFGKRSFTVEALAENFRAVYDAIVAAKPASSKGIYIKRCTVSSTM
                     GPGLHLDVKDKAAA"
     gene            4482..5021
                     /gene="rplJ"
                     /locus_tag="PKI68_03865"
     CDS             4482..5021
                     /gene="rplJ"
                     /locus_tag="PKI68_03865"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNX52859.1"
                     /translation="MSNENKKIRPEKQAMYKEVQENVSGALYMLLADYKGMSMPETDE
                     FKKLLRGANARVKVVKNSMFGRISSEMGCAGFENLSGPTAMIFGAGDVVEVAKILKKF
                     SADKDRPAVKGGVLEGAVLSAKDVIALAGLPGKKELQAKLVGTLAAPMTQVVGVMNQK
                     VCSLLYVLNAVREKKEQAA"
     gene            5117..5482
                     /gene="rplL"
                     /locus_tag="PKI68_03870"
     CDS             5117..5482
                     /gene="rplL"
                     /locus_tag="PKI68_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNX52860.1"
                     /translation="MAEFVDWIEGISVLELSKLVKALETRLGVSAAAPVAVAAAGGAA
                     AAEAVAEKTEFTVVLAQAGSQKIQVIKELRGVTALGLKEAKDLVDGAPKTIKEGVSKE
                     EAATIKAKLEGVGATIEIK"
     gene            5615..9397
                     /gene="rpoB"
                     /locus_tag="PKI68_03875"
     CDS             5615..9397
                     /gene="rpoB"
                     /locus_tag="PKI68_03875"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNX52861.1"
                     /translation="MVERKNFGKLIDVIEAPDLIELQTNSYVEFLQQGVAPSRRKKIG
                     LQAVLSEAFPVESYDGQIALDFVSYEIKEPNMSYVESVKEGETFAAPLYVTFRLRENE
                     DVREDTLFMGEIPLMTDRGSFVINGAERVIVSQLHRSPGICFEQTTHANGSLLYSFRI
                     IPDHGSWVETQFDTSELLYVYLDRKRRRRKFLASTFLRALGYGTDEEIINLYYTIESF
                     PLSKRISDESELSNLVLKDDAVDSDSQSVIGRRYDALTLDMIQRMKLAGYKSVDVVNV
                     AWDEGLFMKTIQKDTTRSVDEALKDLYQKLRPGDPPTTASARQMLKRLFFDETRFSLS
                     RVGRYKIQQKLKTKATSLTLDKEDVVEAIRYLINIRMGNGTLDDIDHLGSRRIRTVGE
                     LLEGQCRVGLARIQRLVKERMTIFDTTVDRLTSQKLINPKALSAVIKDFFGRSQLSQF
                     MDQTNPLSELTHKRRLSALGPGGLNRDRAGFEVRDVHSSHYGRICPIETPEGPNIGLI
                     SSLSTFARINEYGFIISPYRKVEKGRVTDKVESLTADEEENFVIAQANAPLNANGTFQ
                     NDRVMVRVRGDFKEVAPADVQYMDVSPKQVVSIAAGLIPFLEHDDANRALMGSNMQRQ
                     AVPLMVSERPFVATGIEGRIARDSRVLIVAEEAGKVAYVSADKIIVSKDGEAPVKKKT
                     PKEDYQEYDLRKFMRSNAGTCLNQRPIVKHGDKVKKGQVLADGPATDQGELALGRNVT
                     VAFMPWCGYNFEDAILISQKIVKEDVYTSIHIEEFECGARDTKLGPEEITRDIPNVGE
                     EALKNLTHEGIIQIGAEVKPGDILVGKITPKVETELAPEERLLRAIFGEKAADVRDTS
                     LRAPSGTYGIVMDVKVSSKNKNVKEAPKAKPAADMRKRQKDIEERYKSSVHELTEDLT
                     EALSNILLGEKIPLDVMNVQTGDIIIPANRKITKTLLRKLAANYEHIEIDPSPIRIKI
                     MSIIDNFRNKFAEAEADKQRSLDRAGSGEEVDEGIVKQVKVYVASKKKLSVGDKMAGR
                     HGNKGVISRILPEEDMPFLPDGTPVEIVLNPLGVPSRMNVGQVLETHLGWACKHLGIY
                     AATPIFDGISEKEIRQMLEQAGLPKDGKTVLYDGRTGEPFEQRVVVGTIYMLKLHHLV
                     TEKIHARAVGPYSLVTQQPLGGKAQYGGQRFGEMEVWALEAYGAAYALQEVLTVKSDD
                     LSGRTRIYESIVKGENVLDAGRPESFNVLIKELQGLGLNFEVRKPNTDNVSFTGHI"
     gene            9418..13599
                     /gene="rpoC"
                     /locus_tag="PKI68_03880"
     CDS             9418..13599
                     /gene="rpoC"
                     /locus_tag="PKI68_03880"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNX52862.1"
                     /translation="MDTRNAADILGVETEAISEIASIKLASPENIRSWSFGEVKNPET
                     INYRTFKPEKGGLFCERIFGPSKDWECSCGKYKRIKHKGVICDRCGVEVTLSRVRRER
                     MAHIELAVPVSHIWFFKATPSRMGLLLDMTLRNLEHVIYYDDYVVIDPGETPLKEKQL
                     LTELEYREALDNYGDGNFDARIGAEGLRELLKKINLPEKLVELEDALASTRSKQTRKK
                     LVSQMKVIEGLIKSNSRPEWMIFEVLPVIPPDLRPLVPLEGGRFATSDLNDLYRRVIN
                     RNNRLKTLLALKTPEVIIRNEKRMLQQAVDSLFDNGRHGRAVSGPGNRPLKSLSDMLK
                     GKQGRFRQNLLGKRVDYSGRSVIVVGPNLKLNQCGLPKKMALVLFEPFIIRKLKERGI
                     VHTVRSAKKMIEREEEVVWDILDQVTKGHTVMLNRAPTLHRLSIQSFEPVLIEGEAIQ
                     VHPLVCTAYNADFDGDQMAVHVPLSVEAQIESKCLMLAPNNIFSPSSGKPVMTPTQDI
                     ALGIYFLTSFSQTFLLESKKPGAKDEQVHLPLLADINEVHTAFDEGALRLHDRIRYRN
                     PDYQRETKFGDQNKSAIVTTVGRVFFNEVWPSSIGFVNERVTKKLLGKLIEHCYEVSG
                     HDETVRVLDSLKNLGFAQATAAGVSIGLSDMIIPSEKAEIIAAARKIISTVEDKYKKG
                     LITDGERYNMVIDQWTDANDKLTGKLFSALERNDDVTNKDSAKDLNPVFIMVDSGARG
                     SRQQIRQLAGMRGLMAKPSGEIIERPILANFREGLSVLEYFISTHGARKGLADTALKT
                     ADSGYLTRKLVDVAHDIIIMEEDCHTLNSIEVSAITDGDDELVSLAQRIIGRTTCDDI
                     FDPYNNKEVIVAANEIIDEYAAKKIERVGVEKVRIRSVLTCESRRGVCAKCYGRNLAS
                     GREARLGEPVGIIAAQSIGEPGTQLTMRTFHIGGTASSVFKQPKITAKENSVVRYENL
                     RTVKVDNGNVILNKNGYVVTYDDDGRELERYSAVIGAVITIDDGARVKRGDTFVSWDP
                     YSVPILSEQDGTVDFHDFIEGVTVQKAEDETTGIEGLVVLEHKEDLHPQIVVKDASGK
                     AIAYYSIPAAAHVMVTKDMKIRAGATLAKTPRKQSRTKDITGGLPRVAELVEARRPKE
                     AAEIAKIDGVVELGGIAKGKRQLIVRDPATGTEEEHLIPMGKHVIVYSGDVVQKGQQL
                     TEGPIVPQELLEVCGPQELQEYLVNEVQAVYRLQGVEINDKHIEVIVRQMLRKVKITD
                     PGDTEFLWGEQVEKQQFQEVNQRAMEEGRRPAEASPVLLGITKAALATESFISAASFQ
                     DTTRVLTQAATLGRIDQLRGFKENVIMGHLIPAGSGFPLYKNIRPVKLGEEISVEDAL
                     GSNPLMAQDEKTVES"
     gene            13671..>13941
                     /gene="rpsL"
                     /locus_tag="PKI68_03885"
     CDS             13671..>13941
                     /gene="rpsL"
                     /locus_tag="PKI68_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNX52863.1"
                     /translation="MPTINQLIKKPRKPLLKKSKSPALTNCPQRRGVCLLVKTMTPKK
                     PNSALRKIARVRLTNGREINAYIPGEGHNLQEHSMVLVRGGRVKDL"
BASE COUNT         3493 a         3416 c         3907 g         3125 t
ORIGIN      
        1 tgccacgcgg ccggacagac ctggtcgccg tgttcgcgca cgaattttgc ggcttcgagt
       61 ttgcgcaacg cttcaaccgc gctccggccg atgcccaagt cgtgcatttc aacggttttg
      121 agtacgccgt ccggatcgat gatgaacgtt ccgcgcagcg aaagaccttc ggactcaatg
      181 tatgttccga atgcgcggca gatgttgccg gtcggatcgg ctcccatcgg gtatttgacg
      241 gtcttaatgg cttcggattc gtcgtgccag gctttgtgga cgaaggccgt gtcggtgctg
      301 atgctgatga tttccgcgcc ggctttctgg aatttggggt agagcagagc ggcttctttt
      361 aattctgtcg ggcagacgaa agtgaagtct gcgggataaa aaatcagcac aacccatttg
      421 cctttatagt ctgagagttt aattttccgg atttcttcat tgtggaacac gtccatttga
      481 aaatcaggta ccgtttgtcc tatcttgagc ataatattct cctgttaata tgcgaatcgt
      541 ttaaacatga caaacatgat aaccattaat agattgaaat caaggtcgtt ttccatgtat
      601 cacatccggc gaatacaagg acttgatgaa gatcgttaaa aatatttgcc ccaaaatagt
      661 tgacagcatg ttcatttccg ctaaagtcgc tcccctttgt gtgacagacc aagcagttgt
      721 tggtgtttgc gcagagtaat ttaggactaa acgggtatag aaaaagagtc tgtttcttgc
      781 ggtgaaaaaa acaggaagcg ggtcttttta tactttttta tttttgaatg aggatggcgc
      841 gagcacgggg gagaaaccgc ccacgtagct cagttggtag agcacgtcct tggtaaggac
      901 gaggtcagcg gttcaatccc gctcgtgggc tccatagaag aagtggattt ttaactggat
      961 taagtagtag taaacggatc aggaggataa gtcatggcta aggataaatt cgaacggaca
     1021 aaaccgcacg tgaacgtggg tacgatcggc cacgttgacc atggtaaaac aacgctgacg
     1081 gcatcgatca cttgcgtaca gggtgcaaaa ggcttggcta actacatcgc gtacgatcag
     1141 gttgcaaaag catctgagtc tcagggccgc cgtgacccga ccaaaattct gacgattgct
     1201 acttcgcacg tcgaatattc aaccgccaac cgccactatg cgcacgttga ctgccccggc
     1261 catgccgact atgtcaaaaa catgattacc ggtgcggctc agatggacgg tgcaattctg
     1321 gttgtcagcg cctgtgacgg accgatgcct cagactcgcg agcacattct tctggctcgt
     1381 caggtcggcg ttccggcaat tgttgttttc atgaacaagt gtgaccttgt ggatgatacc
     1441 gaactgctcg atctcgttga actcgaaatt cgcgaactgc tcagcaagta cgaatttccc
     1501 ggcgacgaca ctccggttat tcgcggttcc gctacgggtg cgattaagga tccgaccggt
     1561 cctggcgctg cctgcattca ggagttgatg gatgcgctgg acagctacat caaagaaccg
     1621 gttcgtgtta ccgaccaggc gttcctgctg ccgatcgaag acgtattctc gatcgaaggt
     1681 cgcggaaccg tagtaacagg ccgtgttgag cgtggcgtta ttcatcctgg cgatgatgtt
     1741 gaaattgttg gtttgaagcc gacagccaaa accacctgca ccggtgtcga aatgttccgc
     1801 aagcttctgg atgaaggccg ggccggtgac aacgtcggct gcctgcttcg cagcacgaag
     1861 aaggaagatg tcgaacgcgg tcaggttttg gccaagccgg gttcgattct gcctcacact
     1921 cagtttaaat ctgaagtcta tgttctgagc aaagaagaag gtggacgtca tactcctttc
     1981 ttcaaaggct atcgtcctca gttctacttc cgcacaacag acgtaaccgg cgatatcact
     2041 ctgccggaag gtgtggagat ggttatgccg ggtgacaatg tggcgatgga agttacgctg
     2101 atcaccccga tcgcaatgga gcagcacatg cgcttcgcga tccgtgaagg cggacgcacc
     2161 gttggtgccg gtcgtgtgac cgaggttctt aaataagtta agtgtccgcg atgctgacgg
     2221 ggtgatgtgt ctgttataag gcacatcact ttgtcggcga tctgatggga aagagtaaaa
     2281 tcatgcgaga tattattaca ttggcctgta cagagtgtaa gcgccgtaat tatacgacga
     2341 cgaagaataa gaagctttcg acgggccggc ttgaagttaa aaaattctgc ccgcatgacc
     2401 gcagtcacac cgtccataag gaaacaaagt aactttaaag atttcggtgt aggccagtag
     2461 ctcaattggc agagcggcgg tctccaaaac cgcaggttgg gggttcaatt ccctcctggc
     2521 ctgccaagtt cttcagggaa acgagatgaa aaaaattatc gaactgttta aagcgctgaa
     2581 gacgttcctg agcgaggtga aaatcgagct taagaagtgt acttggccta ctcgtcagga
     2641 gttgttcggt tcgacgatgg tcgtggtgat ctctgtggtg attcttggtg ttttcgtcgg
     2701 gttgagcgat acagtactga tgggccttct gcgggcggtc ttgcgttaaa cggagagaaa
     2761 gacactaatg gaaaaacact ggtttgtttt acatacgctc tccgggcatg aatataaggt
     2821 gcagaacagc atcgtcagcc gggtgaagca ggaagagatg ggggaataca tcggcgaagt
     2881 cctgattccg accgaaaaag tttcggaagt taagcagggc aaaaagacca cagtgtcgcg
     2941 caagtttttc cccggctatg tgctggcaaa tctggcattg tttgacgaaa agaaaaattt
     3001 aatagaagaa acctggtatt tcatccagaa cacgcccggc gtgatcggtt ttgtcggagg
     3061 cgaggctccg gtaccgttgc gcgctgatga ggttgaccgg atcattaacc aggtggaaga
     3121 gaaaaaagaa acggttacac ctaaaattaa ttttgaaccg ggtgagacca tcaagattac
     3181 tgatggaccg ttcctgaact ttaacggagt gattgaagaa gttgatccgg gccgcggtaa
     3241 actgaaagtg tcggtttcga ttttcggtcg atcggctccg gttgaacttg aatattggca
     3301 ggcggataaa gcgtaacctg agcggagttt tgttatggcg aaaaaagaaa aaggcatgat
     3361 caagttgcag attccggccg gacaggccaa cccggcaccg ccggtaggtc ctgcgctggg
     3421 tcagcacggt gtgaacatta tggagttctg taaggctttc aatgcagcta cgcagaaaga
     3481 agcgggcatg attattccgg tggtaatcac cgtttatcag gatcgctcgt tcagttttat
     3541 caccaagagc ccgccggctt ccgctttgct taaaaaagcg gctgctgtag ctaaaggatc
     3601 tggcgttccg aaccgcgata aagtgggcaa agtgaccctc gcgcaagttg aagaaattgc
     3661 caaaattaaa gaggctgatt taaacgccag tgacagagcg gcagcttgcc gtattatcgc
     3721 cggcacagcc cgcagtatgg gaattgaggt agaatagcca tggctacaca cggaaaaaaa
     3781 tataccgcag taaagaagaa tgtttcggtc gaaaaactgt ataccgttga agaagcgata
     3841 cagttcatca aagataatcc gaccgctaaa tttgacgaaa ccatcgaagt agccctgcgt
     3901 atgggtgtcg atccgacgaa gtcggatcag gcaatccgtt ccactgttgc tcttccgaac
     3961 ggaaccggta aggacgttcg cgtaatcgta tttgcctccg gcgccgctgc tgaagccgcc
     4021 cgtgctgcag gcgccgttga agccggattt gaagatctgc tggccaaagt gaccggcggc
     4081 tggatggatt ttgatattgt tgtttccact ccggatgcga tgaaagaagt ccgcaagctc
     4141 ggtaaagtgc tcggccctcg cgggttgatg cccaacccga agaccggtac ggttacggac
     4201 gatacagcca cagccgttaa gcagtttaaa gccggtcgcg ttgagttccg tatggataaa
     4261 aacgggaacg ttatcgttcc tttcgggaaa cgctccttca cggtcgaagc gctggctgaa
     4321 aacttccgcg cagtttacga cgcaatcgtg gccgcgaaac cggcttcgag caaaggtatt
     4381 tatatcaaac ggtgcacggt ttccagcacg atgggaccgg gtctgcatct tgatgtgaaa
     4441 gataaggcag cagcttaaat ttgtaaggac gggcgaaagt catgagcaac gaaaataaaa
     4501 agatcagacc ggaaaagcag gcgatgtaca aagaggttca ggagaatgtc tccggagcgc
     4561 tttatatgct gctggctgac tacaagggca tgagcatgcc cgagaccgat gaattcaaaa
     4621 agcttctgcg cggtgccaat gcacgggtga aagtggttaa gaacagcatg ttcggccgca
     4681 tcagcagcga aatgggctgt gccggttttg aaaacctgag cggtccgacg gcgatgattt
     4741 tcggtgccgg tgatgtcgtt gaagtggcga agatcctgaa gaaattttct gcagataaag
     4801 acagaccggc tgttaaaggc ggagtgcttg aaggcgccgt tctttctgcg aaggatgtaa
     4861 ttgcgctggc cggtctgccc ggcaaaaaag agcttcaggc taaactggtc ggcactctgg
     4921 ccgctccgat gacccaggtt gtcggcgtaa tgaatcagaa agtttgcagt ctgctgtatg
     4981 tgctgaatgc ggttcgcgag aaaaaagagc aggcggcata aataacgttt ttaaattagg
     5041 ttggttggat tcaattcatt gtagggagga agataaaatg gctgaagaaa ctgtagaagt
     5101 gaaacttgaa ggcaaaatgg ctgagttcgt tgactggatc gaaggcattt ctgtgctcga
     5161 actttcaaaa ctcgtcaaag cactggaaac ccgtctgggc gtttccgcag cagctccggt
     5221 cgcagtcgcg gcagcgggcg gcgcggcagc ggcggaagcg gttgctgaaa agactgaatt
     5281 cacggttgtt ctggcgcagg ccggttcgca gaagatccag gttattaaag agctccgcgg
     5341 cgtaacggcc ctgggtctca aagaagccaa agatctggtg gacggcgctc ctaagacaat
     5401 caaagaaggt gtctcgaagg aagaagccgc gaccatcaaa gctaaactcg aaggcgttgg
     5461 cgccacaatc gaaatcaagt aatgatgcga tatgcgttcc gttccggcgg ccttttgcgc
     5521 agccggcacg gtcgcgtctc ggatttcgtg tgcccggatg ctccgtatgg agttctctgg
     5581 gcgaagcgtc caataagagc ctgaaggtga tggaatggtc gagagaaaaa actttggaaa
     5641 actgatagac gtaattgaag caccggatct tatcgagctt cagaccaatt cctatgtgga
     5701 atttctccag cagggagtcg ccccgtcacg ccggaaaaaa atcggtctgc aggcggttct
     5761 gagcgaggcg tttccggttg aaagctatga cgggcagatt gctcttgatt tcgtaagcta
     5821 cgaaatcaaa gagccgaaca tgtcatatgt tgaatctgtt aaagagggcg agacctttgc
     5881 ggctccgctc tatgttacgt tccgtctgcg tgaaaatgaa gatgttcgcg aagacaccct
     5941 gtttatgggt gaaattcctt tgatgacaga tcgcggaagc tttgtcatta atggcgctga
     6001 acgcgttatc gtcagtcagt tgcaccgctc accgggtatc tgttttgaac agaccaccca
     6061 tgcgaacggt tcacttctct attcattccg tattattccg gaccacggct catgggtcga
     6121 aacacagttc gacacttcag agcttctata tgtctatctg gaccgcaaac gccggcgcag
     6181 aaaattcctt gcgtcaacct tcctgcgcgc gctcggttac ggcacggatg aagaaataat
     6241 taatttgtat tacacgattg aaagtttccc gctgagcaaa cgcatcagcg acgaatcgga
     6301 actttccaat ctggttctta aagacgacgc ggttgacagc gactcgcagt ctgtcatcgg
     6361 ccgccgttac gatgcgctca cgctggatat gattcagcgg atgaagcttg ccggctacaa
     6421 gagcgtcgac gtggttaatg ttgcctggga cgaaggtctt tttatgaaga ccattcagaa
     6481 agacaccacc cgctcggttg acgaagcgct caaagacctc tatcaaaagc tccggcccgg
     6541 cgatccgccg acaaccgctt ccgcccgcca gatgctcaaa cggctgtttt tcgatgaaac
     6601 ccgtttcagt ctgagccgtg tcggccgcta taagattcaa cagaagctga aaacaaaggc
     6661 aacttctctg acgctggata aagaagatgt agtggaagcg atccgctacc tcatcaacat
     6721 ccgcatgggc aacggtacgc ttgacgatat cgaccatctg ggaagccgcc gcatccgtac
     6781 ggttggtgaa ctgcttgaag gacagtgccg cgtcggtctg gcccgcatcc agcgtctggt
     6841 caaagaacgc atgacgatct ttgatacaac cgttgatcgt ttgacttcgc agaagctgat
     6901 caatcccaaa gcactctctg cggtgatcaa ggatttcttc ggccgctcgc agctcagcca
     6961 gttcatggat cagaccaatc cgctgtccga actgacccac aagcgccgtc tgtcggcgct
     7021 cggcccgggc ggtttgaacc gcgaccgcgc cggtttcgag gtccgcgacg ttcacagttc
     7081 gcactatgga cgtatctgcc cgattgaaac gccggaaggt ccgaacatcg gtcttatttc
     7141 ctcgttgagc acgtttgcgc gtatcaatga atacggtttt atcatttccc cttaccgtaa
     7201 ggttgagaag ggccgtgtga ccgacaaagt ggagtcgctg actgcggatg aagaagaaaa
     7261 tttcgttatc gctcaggcga atgctccgct caatgcgaac ggcacattcc agaatgatcg
     7321 cgtgatggtt cgtgttcgcg gcgactttaa agaagttgct ccggccgacg tgcagtacat
     7381 ggacgtgtct ccgaagcagg ttgtcagtat cgctgccgga ctgattccgt tccttgagca
     7441 cgacgacgct aaccgcgcct tgatgggctc gaacatgcag cgccaggcgg ttcctttgat
     7501 ggtatccgag cggcctttcg tggccaccgg tatcgaaggc cgcatcgccc gcgactcgcg
     7561 cgttctgatt gtcgcggaag aggccggtaa agtggcttac gtttctgccg ataagattat
     7621 tgtttctaaa gacggcgaag cgccggtcaa aaagaaaaca cccaaagaag actatcagga
     7681 atacgatctg cgcaagttca tgcgttcaaa cgcaggaacc tgtctcaatc agcgcccgat
     7741 tgtcaaacac ggcgacaagg ttaaaaaagg acaggtgctc gcggacggtc ctgcgaccga
     7801 tcagggcgaa ctcgcgctgg gtcgcaatgt gactgtcgcg tttatgccgt ggtgcggata
     7861 caactttgaa gacgcgattc tgatcagtca gaaaatcgtc aaggaagacg tttacacctc
     7921 aattcatatc gaagaattcg agtgcggcgc ccgcgatacc aaactcggtc cggaagaaat
     7981 cacccgggat attccgaatg tcggcgaaga ggctctcaag aacctgacgc acgaagggat
     8041 tattcagatc ggtgcagaag tgaagcccgg cgatattctg gtcggtaaaa ttacaccgaa
     8101 ggttgaaacc gagctggctc ctgaagaacg tctgctgcgc gctattttcg gcgaaaaagc
     8161 ggccgatgta cgtgatacat cgctgcgtgc gcccagcgga acgtacggca tcgtcatgga
     8221 tgtcaaagtt tcttcgaaga acaagaacgt caaagaagcc cccaaggcca agccggcggc
     8281 ggacatgcgc aaacgccaga aggatattga agaacgctat aagagttcgg tgcatgagtt
     8341 gaccgaagat cttacagaag cgctcagcaa cattctgctc ggtgaaaaaa ttccgctgga
     8401 tgtgatgaat gtgcaaaccg gcgatattat tatcccggca aaccgcaaga tcaccaagac
     8461 gctgctgcgc aaactggcgg ccaattacga acacatcgaa attgatccca gcccgatccg
     8521 cattaagatc atgagcatca tcgacaactt ccggaacaaa tttgcggaag ccgaagctga
     8581 taaacagcgc tcactcgacc gcgccggaag cggtgaagaa gtcgatgagg gtatcgtcaa
     8641 gcaggtcaag gtttatgtgg ccagcaagaa gaagctgtcg gtcggtgaca agatggccgg
     8701 acgtcacggt aacaagggtg ttatctcgcg cattctgccc gaagaggaca tgcctttcct
     8761 tccggacgga actccggtcg aaatcgtgct caacccgctc ggcgtgcctt cacgtatgaa
     8821 cgtcggacag gttcttgaaa cccaccttgg atgggcttgc aagcatcttg gaatttacgc
     8881 ggcgacgccg attttcgacg gtatttctga aaaagaaatc cgccagatgc tcgagcaggc
     8941 cggactgccc aaagacggca aaactgtgct ctatgacgga cgtaccggtg agccgtttga
     9001 acagcgcgta gtggtcggaa ccatttacat gctcaaactg caccacctgg ttaccgagaa
     9061 gatccatgcc cgtgcagtcg gtccgtattc gcttgtcacc cagcagccgc tgggcggtaa
     9121 ggctcagtac ggcggtcagc gcttcgggga aatggaagtg tgggcactgg aagcttatgg
     9181 cgcggcgtac gcattgcagg aagttctcac ggtcaagagc gatgacctct ccggccgtac
     9241 ccgaatttat gagtccattg tgaagggtga gaacgtgctc gatgccggac gtccggaatc
     9301 gttcaacgtg ctgatcaagg aactgcaggg gctcggcttg aattttgaag ttagaaagcc
     9361 gaacacagat aatgtttctt tcacagggca tatttaatct ttgacaggag aacaccggtg
     9421 gatacacgca acgcagcaga catactcggc gtagagacag aggcgatcag tgaaatcgcc
     9481 agcatcaagc tggcttcgcc cgagaacatt cgttcctgga gcttcgggga agttaaaaac
     9541 cctgaaacca tcaattaccg gacgtttaag cctgagaagg gcggactttt ctgtgaacgc
     9601 atttttggtc cttccaagga ctgggagtgc agctgcggaa agtacaagcg catcaagcat
     9661 aaaggcgtga tttgcgaccg ctgcggcgtc gaagtgactc tgtcacgcgt tcgtcgcgaa
     9721 cgcatggctc atattgagct ggctgttccg gtttctcata tctggttctt taaagcgacg
     9781 ccgagccgta tggggctcct gctggacatg accctgcgca atctcgaaca cgtcatctac
     9841 tacgacgact atgttgtgat tgatccgggc gaaacgccgc ttaaggagaa acagctgctg
     9901 acggaacttg aataccgtga agcgctggat aactacggcg atggcaattt tgatgcccgt
     9961 atcggtgccg aagggcttcg tgagctgctg aagaaaatca acctgcccga aaaactggtt
    10021 gagctggaag atgctctggc cagcacccgc agcaaacaga cccgcaagaa actggtcagc
    10081 cagatgaagg tcatcgaagg gcttatcaag agcaattcgc ggcctgaatg gatgatcttt
    10141 gaagtcctgc cggttattcc tccggacctt cgtccgctgg ttccgcttga aggcggacgt
    10201 ttcgcaacgt cagacctgaa tgatctttac cgtcgcgtga tcaaccgcaa caaccgtctg
    10261 aaaacactgc tggccctcaa gacaccggaa gtgattatcc gtaacgaaaa gcggatgctg
    10321 cagcaggcgg ttgactcgct gttcgataac ggccgccacg gccgtgccgt cagcggtccg
    10381 ggcaaccgtc cgctgaaatc gctgagcgat atgctcaaag gtaagcaggg ccgcttccgt
    10441 cagaatctgc tcggtaagcg cgttgactac tccggccgtt cggtcatcgt ggtcggaccg
    10501 aacctgaagc tgaatcagtg cggtctgccg aaaaagatgg ctctggttct gttcgaaccg
    10561 ttcattatcc gcaaactcaa agagcgcggc attgtgcaca cggtgcgcag cgccaagaag
    10621 atgattgagc gcgaagaaga agttgtatgg gacattctcg accaggtgac caagggtcat
    10681 acggtcatgc tgaaccgtgc gccgacgctg catcgtctgt cgattcagtc cttcgagccg
    10741 gtgctgattg aaggggaagc cattcaggtg cacccgctgg tctgtacggc ctataatgcg
    10801 gacttcgacg gtgaccagat ggccgttcac gttccgctgt ccgttgaagc gcagatcgaa
    10861 tcgaaatgtt tgatgctggc tccgaacaat attttctccc cgtccagcgg taagccggtt
    10921 atgaccccga cgcaggatat tgcgctcgga atttacttcc tgaccagttt ctcgcagacc
    10981 ttcctcctgg aatccaagaa acccggcgcg aaagacgagc aggtgcatct gccgcttctc
    11041 gcggatatca acgaagtgca cacagccttt gatgaaggcg ctctccgtct gcacgaccgg
    11101 atccgctacc gcaatccgga ttatcagcgg gaaaccaagt tcggcgacca gaacaagtcc
    11161 gccattgtta caactgtcgg acgtgtattc tttaatgaag tctggccgag cagtatcggt
    11221 tttgtgaacg aacgggttac caagaagctc ctcggtaaac tcattgagca ctgctatgag
    11281 gtttccggcc atgacgaaac ggtccgtgta ctggacagcc ttaagaatct cggatttgcg
    11341 caggcaacag ccgcaggtgt ttcgatcggt ctttccgata tgatcattcc gtcggaaaaa
    11401 gcagaaatta ttgctgccgc ccggaaaatc atcagcacgg tggaagacaa atataaaaag
    11461 ggtcttatca ccgacggcga acgttacaac atggtcattg accagtggac cgacgccaac
    11521 gataagctga ccggcaagct gttcagtgcg ctggaacgca atgacgatgt gacgaacaag
    11581 gattcggcta aagaccttaa tcctgtgttt atcatggtgg attccggagc gcgcggcagc
    11641 cgccagcaga ttcgtcagct ggccggtatg cgcggcttga tggctaaacc gtccggcgaa
    11701 attattgagc gtccgatttt ggcgaacttc cgcgaagggt tgagcgttct cgaatacttt
    11761 atttcgaccc acggtgcccg taaaggtttg gccgataccg cgctcaagac ggcggactcc
    11821 ggttacctga cccgcaagct ggtggacgtc gcccatgaca tcatcatcat ggaagaagat
    11881 tgtcatacgc tcaacagcat tgaagtaagc gcgatcaccg acggtgacga cgagcttgta
    11941 tcgctggccc agcgtattat cggacgtaca acgtgcgatg atattttcga cccgtacaac
    12001 aacaaagaag tgattgttgc ggcgaatgaa atcatcgacg aatacgctgc caagaagatc
    12061 gaacgggtcg gcgttgaaaa ggtacgcatc cgcagcgtac tcacctgtga atcgcgccgc
    12121 ggcgtttgcg ctaaatgcta cggacggaat ctggcgtccg gccgcgaagc ccgtctgggc
    12181 gaacctgtcg gtattatcgc cgcacagtcc atcggtgagc ccggaacgca gctcacgatg
    12241 cgtacgttcc acatcggcgg taccgccagc tcggtcttta aacagccgaa aattacggct
    12301 aaggaaaact cggttgtccg ttacgaaaat ctccgtaccg ttaaggtcga taacggcaac
    12361 gttatcctca ataagaacgg ttatgtggtg acctatgatg acgacggccg cgaactcgaa
    12421 cgttactctg cagttatcgg cgctgtgatc acaatcgacg acggtgcccg tgttaagcgc
    12481 ggcgatacgt ttgtcagctg ggatccgtac agcgtgccga ttctttcgga gcaggacggt
    12541 acagtcgatt tccatgactt tattgaaggc gtcacggttc agaaggccga agacgaaacg
    12601 accggcattg aaggtctggt tgtgctggag cacaaagaag acctgcatcc gcagattgtt
    12661 gttaaagacg cgtccggcaa ggcgattgcc tattactcga ttcctgccgc ggctcacgtg
    12721 atggtgacga aggatatgaa gatcagagcc ggtgctacgc tggcgaagac gccccgtaag
    12781 cagtcccgta ccaaggacat taccggcggt cttccgcgtg tggccgaact ggtcgaagcc
    12841 cgccgtccga aggaagctgc ggaaatcgcg aagattgacg gggttgttga attgggcggc
    12901 atcgctaaag gaaaacgtca gctgatcgtt cgtgacccgg ctaccggaac ggaagaagag
    12961 catctgattc cgatgggtaa acacgttatc gtttactccg gcgatgttgt tcagaaaggc
    13021 cagcagctca cagaaggtcc gattgttccg caggaattgc ttgaagtttg cggtccgcag
    13081 gaattgcagg agtatctggt gaatgaagtg caggcggtgt accgtctgca aggtgtggaa
    13141 atcaacgaca agcatattga agtgattgtt cgtcagatgc tgcgcaaagt gaaaatcacc
    13201 gatccgggcg acaccgaatt tctgtggggc gagcaggttg aaaaacagca gttccaggaa
    13261 gtcaatcagc gggctatgga agaaggacgc cgtccggcag aagcttcccc ggtactgctg
    13321 gggatcacca aagcggctct ggccaccgaa agctttatct cggcggcatc gttccaggat
    13381 accacccgcg tactgacgca ggcggcgaca ctgggccgta tcgaccagct gcgcggtttc
    13441 aaggaaaacg tgattatggg tcatctgatt cctgcgggaa gcggattccc gctttacaaa
    13501 aatattcgcc cggtcaagct gggcgaggaa atcagcgtag aggatgcgct gggttcgaac
    13561 cctttaatgg ctcaggacga aaaaaccgta gaaagttaaa tcttaatgct tgcgggatga
    13621 gcggccggct cctattatct gccgctcgtt ttttttgatg gaaggaacat atgccgacaa
    13681 ttaaccagtt gattaaaaaa ccgcgtaaac cgttgctcaa gaagagcaaa tcgcctgcac
    13741 tgactaactg cccgcagcgt cgcggcgtct gtcttctcgt gaaaaccatg accccgaaga
    13801 agccgaactc cgcgttgcgt aaaatcgccc gcgtgcgttt gacgaacggc cgtgaaatca
    13861 acgcctacat tccgggtgaa ggacataatc tgcaggaaca cagcatggtg ctggttcgcg
    13921 gcggaagggt gaaggacttg c
//
LOCUS       DAOPZO010000037        13437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_5058_length_13437_cov_8.638993, whole genome shotgun sequence.
ACCESSION   DAOPZO010000037 DAOPZO010000000
VERSION     DAOPZO010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 13437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13437
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5058_length_13437_cov_8.638993"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="PKI68_03890"
     CDS             complement(<1..1322)
                     /locus_tag="PKI68_03890"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNX52864.1"
                     /translation="MNTKTDPSLAITRRNALQLGMFGAAGLMLSDLFTLKAAGAINPL
                     KAKAKSVIQVWLWGGPSHLDSFDPKPEAGYDYCGKFDKPIDTNVSGIRIGQTLPLLAK
                     MADKYSLLRGMTHGSNAHEIASYMVMTGTKPVDGIVNPSVGAVLSFKKGYGAGYEGLI
                     PPYVALTKPQGRFSEAGYLGSKYQPFATGGDPAKTPFTVEGVVAEGITDTRQQGRRTL
                     LENLDTFALAMEGNPLIDQMKKNKQDAYSLILGKARSTFDLSKEPEALRTSYGKNTFG
                     QSCLQARKLVEAGVPFVTINSPGWDTHKKGFEVLTQKLNELDKGLSTLLQDLSSRGLL
                     DSTIVWCGGEFGRTPKVDWEAPWNGGRGHYGKAFSHFVAGGGFRGGHVVGSTDLRGEE
                     VATRPIYPWDLTASIYELLGIDFNSKLPTPDGKTEFFSPLADSSIKVQ"
     gene            complement(1337..2890)
                     /locus_tag="PKI68_03895"
     CDS             complement(1337..2890)
                     /locus_tag="PKI68_03895"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HNX52865.1"
                     /translation="MKHMVRAFLLLVVVLVPFGSQAVSPQNQIDKLVFAKQSETGFPP
                     SELCSDAVFIRRVYFDVIGTLPTADEVRKFLSSRNPNKRSELIEELFERPEFADYWTL
                     KWGDLLRIKSEFTSNLWPNAVQAYQIWLRDAFRRNMPYDQFARALLTASGSNFREAPA
                     NFYRPFQERTPRKMFDTVALIFMGVRLEKSGWTEDQLLGMDAFFAKVAYKKTDEWKEE
                     IVFSDPEKTLLNPATKTPVLPTPIGGSPLKLGHFDDPRLAFADWLTAPDNPWFAKAIV
                     NRIWFWMMGRGIIHEADDIRPDNPAWSPELLAYLEKELVESRYDLRHIYRLILNSATY
                     QLSSLPTSGNLADEAGFSHYRARRLDAEVLIDAICQITGTGEEYSSAIPEPFTFIPSS
                     RRTITLADGSIKSSFLQMFGRPGRDSSLESDRNNNVSVFQTLHLLNSSHIQNKVMKGP
                     RLRKLMTLAPGSKDRVNLLYLEILSRMPSPEEQKIAMDYISSSDRSENGFYDLAWALI
                     NTSEFILKH"
     gene            complement(3042..4289)
                     /gene="rho"
                     /locus_tag="PKI68_03900"
     CDS             complement(3042..4289)
                     /gene="rho"
                     /locus_tag="PKI68_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017631298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNX52866.1"
                     /translation="MKLFELQVTEIPELKKLAESYSIEDPAGLRKHELIFEILKTQIR
                     WGNEALCRGILEVLPDKFGFLRSSENNYLPNPEDIYVSPSQIRRFGLRTGDLIEGPIR
                     SPRPKERFFALLSVDKVNNNAPEKNLTRIPFENLTPVFPDERLGLETTADEISMRVMD
                     LATPIGKGQRGLIVAPPRTGKTVLLQKIANSISANNPDAKLIILLIDERPEEVTDMQR
                     TTKAEVLSSTFDEHPERHVQVAEIVIEMAKRRVENGEDVIILLDSITRLARAYNTIQP
                     HSGKILSGGVDANALHKPKRFFGAARNIEGGGSLTIISTALVDTGSRMDEVIFEEFKG
                     TGNMELALDRDLVGKRIFPAINIEKSGTRKEELLLHKEELARIWTLRKALKDVPAVEA
                     MELLINRLKKTKSNAEFLMALQG"
     gene            complement(4394..4612)
                     /locus_tag="PKI68_03905"
     CDS             complement(4394..4612)
                     /locus_tag="PKI68_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52867.1"
                     /translation="MTTTGWMTAGCARTMIAGRMIVDTGRMTAGCVMIMDTGRMIADH
                     VRITVLVRIIIVGRIITIATIPVITGAT"
     gene            complement(4786..5409)
                     /gene="coaE"
                     /locus_tag="PKI68_03910"
     CDS             complement(4786..5409)
                     /gene="coaE"
                     /locus_tag="PKI68_03910"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNX52868.1"
                     /translation="MNSPQSIILGITGGIACGKTETGRILSSEGFKVLDSDFLAHELM
                     GKGRSVYAAVVDYFGDEILAADGEIDRVKLGKKVFDDPTAREALNRLVHPAVIAAGQE
                     WIAACREAQEDAAVLVPLLFEAGWTKGWDAVICVTAPQDQVLQRLEKRGMLKETARKR
                     IAAQMPQSEKAARADFVVENGGTPEMLRSRIMSVVEKIRCEKRNIYE"
     gene            complement(5533..5937)
                     /gene="ruvX"
                     /locus_tag="PKI68_03915"
     CDS             complement(5533..5937)
                     /gene="ruvX"
                     /locus_tag="PKI68_03915"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNX52869.1"
                     /translation="MGRVLGIDHGDVRIGVALSDPTEFLASSLCVLDSTNAGLDQIIA
                     LIAEHQVEKIVVGLPRNMDGSYGSATEKVRNFIEKLKLRTDVPVFEWDERMSTVSAHN
                     ALREAGLNGKRRKGVVDKVAAQIILQNYLDAQ"
     gene            complement(5937..6413)
                     /locus_tag="PKI68_03920"
     CDS             complement(5937..6413)
                     /locus_tag="PKI68_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52870.1"
                     /translation="METWLREFSGLLLHFFGRATPNETYLVVALCVMFGALALSRVST
                     SLGAIGAFYTTGVLLTSSGLMIIIAALALLPFLGFQAWWLPLAVPAAAVLVIVLPLTV
                     LFQKGSYVTALIGWTVTLLVVAAILTLEPIGRHAIEKGLEKTKPLERHRIETEHYK"
     gene            complement(6428..7510)
                     /locus_tag="PKI68_03925"
     CDS             complement(6428..7510)
                     /locus_tag="PKI68_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNX52871.1"
                     /translation="MSGRFAVIMAGGKGERFWPLSTSKHPKQLLALAGDKALIAQAVE
                     RLDGLIPPENVFVVTNADLVEATRAAAPMLPPENIVGEPIGRDTAAAVACGGALVGAR
                     DPKAVFAVLTADQVMGDLDIFKATLRGGMALAGENEILVTIGIQPAFPSTGFGYIESG
                     ADFAKAGGVQFKKAVRFVEKPALAKAQEYLASGRFFWNSGMFIWSVPTLAKAFAMHCP
                     EMKTLMDELTTYAKRGEISVGLEKIYPALGKISVDYALMEKADNIVMACGTFAWDDVG
                     SWPALESHFPQDADGNTLIGTCEQIDSKNNIIYSKDRLTAVIGAENLIVVQADGVTLV
                     CPKEKAQEIKKMLARLRERGSYDHLL"
     gene            complement(7687..8553)
                     /locus_tag="PKI68_03930"
     CDS             complement(7687..8553)
                     /locus_tag="PKI68_03930"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNX52872.1"
                     /translation="MNLSNERKLAARAKELGESASSASFAELAELTRSESPLVRRLAA
                     SALGKLAGIVAPESAVKTLQPLLSDVHPQVRQYAAKALGAFGTHAKDVLPDLRDLYKS
                     PVEKDYVKRSVLAAGKTIREAVLIAEEQVVHCCRRCGVKLASDEYARSQKAFQRPFCD
                     HCFDEVFLDRRNFETKVELQKTIRAKDGTIVQSDGERRICEVLATEGIQYRYDERFRI
                     LSGHAIRPDFYLPEFDVYVEYWGMDTADYKIGMLKKQQLYQQQGKKLISLYPDDKPRL
                     RDVLLAKLEKLK"
     gene            complement(8619..9425)
                     /gene="trpA"
                     /locus_tag="PKI68_03935"
     CDS             complement(8619..9425)
                     /gene="trpA"
                     /locus_tag="PKI68_03935"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNX52873.1"
                     /translation="MKKTRVDQKFADLKKQGKKGFIAYIGAGDPTLDDTVDIVLRLEE
                     AGVDLVELGLPFSDPLADGRVNQDSATRALAAGATFDGVMEMVRKIRERSQIPMIFYA
                     YLNTLYARGFEKAMNEASTAGIDGFLVLDMPREEAGPYRKFISGANLNNISLITPTTP
                     EERMKQIVKDANGFVYCVSREGVTGVRDSIAEGARGLVGKIKTHTRLPVALGFGIGTP
                     AQAAEAASFADAVVVGSAIVNKFYNNPHTPEGRADAAAFVAEIVRAVKAV"
     gene            complement(9422..10471)
                     /locus_tag="PKI68_03940"
     CDS             complement(9422..10471)
                     /locus_tag="PKI68_03940"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: protein motif:HMM:NF002194.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HNX52874.1"
                     /translation="MTLDGLLQRHGGWLETGPDEGPVISSRVRLARNLRDTTFPGWAS
                     KDVRNRVWNEVLLAFDGVPDSKSFLRWRMDELSALDRELLFERHLISRELADRQTGSG
                     VFVNEDECRAVMVNEEDHIRLQSLQSGMNLQKAWEVADELDNALEQSLSYAFSSKLGY
                     LTACPSNVGTGLRASVMLHLPGLVLTEEIKPVINAVSKIGLTVRGLWGEGSEASGHMF
                     QISNQITLGRPEPEIISNLEQIVLEVIEHEKNARIRLMSTQEIRVQDHVGRAYGILAN
                     AALMTTGEALDLLSALRMGVDFGLLPKMVRREIDRLFIQIHPAHLQKEAGSALSPEER
                     DIKRALLIRKFLEKK"
     gene            complement(10468..10974)
                     /locus_tag="PKI68_03945"
     CDS             complement(10468..10974)
                     /locus_tag="PKI68_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HNX52875.1"
                     /translation="MKCDLCDKEAVVHLTQVVNGEMKEVHLCEEHAKAQGIDLNSPIS
                     ITDILMGLSGPQKNIASELSLACPRCGMAREEFRKTGRLGCPDCYKTFMAELTMAIKA
                     MHHSGQHFGKIPAREGVQTRMKSKIARLQKELEAAIAHEDFEKAAKLRDQIKKCREDG
                     TPGEGGAK"
     gene            complement(10985..11854)
                     /gene="ilvE"
                     /locus_tag="PKI68_03950"
     CDS             complement(10985..11854)
                     /gene="ilvE"
                     /locus_tag="PKI68_03950"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006185.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HNX52876.1"
                     /translation="MKIFLNGKLVDEKDAVVSVFDHGLLYGDGVFEGTRVYNGRVAFL
                     DEHMDRLLDSAKVINLNVGMTKDELKKATIETCKANGLKNGYMRHVVTRGVGSLGLNP
                     FQCKQAQVIIIAADIQLYPAELYEKGMKIITAGTIRNMAEAVNPRVKSLNYLNNIMAK
                     IEAINAGVMECLLLNSQGFIAEASGDNVFAIKGNKLMTPPTWCGALEGVTRDKVMQLG
                     RELGMDVQETVMTRYELWTADEVFLTGTAAEVIAVVELDKRIIGTGRPGPATNKLIKA
                     YRDFVNNNGTPIE"
     gene            complement(11864..12538)
                     /locus_tag="PKI68_03955"
     CDS             complement(11864..12538)
                     /locus_tag="PKI68_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX52877.1"
                     /translation="MSKTILRAEEVHKTYHIGKRAVEVLHGVTLTINRGETVSIMGAS
                     GAGKSTLMHILGGLDRPDSGDIFFEETSLVKMNPAKRAAFRSKRCGFIFQSYHLLPEL
                     DVRQNVILPSMAAGGGREVLARAEYLLEQVGLAGRMDHRPMELSGGEQQRVAIARALM
                     NDPDLILADEPTGNLDSHTGENILNNLFDLAQSAQRTLIMVTHNEEVARRCQRELVLK
                     DGKLEE"
     gene            complement(12541..>13437)
                     /locus_tag="PKI68_03960"
     CDS             complement(12541..>13437)
                     /locus_tag="PKI68_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX52878.1"
                     /translation="RCDEACAVLARTVQLEPSCEDCWSDWGGVLRCMGRYEEALEKHN
                     EACRLDPSSAYDIHSAGYCLEQLGRDAEMIEHYRHAAEMGYAKGQYALGWCYQNGRGV
                     EKDLVKAFDWYRKAAAQQNDDAQLSLGYFCETGLGVEKDMQEALKWYRMAADQGNVTA
                     LCNLALCYSKGKGVEKNLQQAVLYYRKALEIQPDHATALNGYAWFLATCDDLSLRNYP
                     EAIRLAELSVSKEEQIFNLDTLAVAYENNGQYAKAAEAQKRLIAFRKKISPDKPISDK
                     ALSRLAKYEKLAAEQAAKAQEQ"
BASE COUNT         3022 a         3973 c         3271 g         3171 t
ORIGIN      
        1 ctttgaactt taatactgct atcggccagc ggggagaaaa actctgtttt cccgtccggt
       61 gtaggcagct tgctgttgaa atcgataccc agaagttcat aaatgctggc cgtcagatcc
      121 cacgggtaaa tcggccttgt cgcaacttct tcaccccgca aatcggttga gcccacaaca
      181 tgtccgcctc taaacccgcc gcccgcaaca aaatgggaaa atgctttccc gtagtggccg
      241 cgtccgccat tccagggagc ttcccagtcg actttcggcg tgcgtccgaa ttctccgccg
      301 caccacacaa ttgtagaatc caacagtccg cggctcgaaa gatcctgcaa aagagtcgaa
      361 agacctttgt ccagttcgtt gagtttctgc gtcagcactt caaagccctt cttgtgggtg
      421 tcccatccgg gagaattgat cgtgacaaac ggcaccccgg cctccaccag tttacgggcc
      481 tgaaggcagg actgcccaaa cgtgttcttg ccatacgaag tccggagcgc ttccggttct
      541 ttggataaat cgaacgtgct tctggccttg ccaagaatca gagaataagc gtcctgttta
      601 ttctttttca tctggtcgat aagcgggttg ccttccatgg cgagcgcaaa cgtatcgagg
      661 ttttcaagca gtgtgcgccg cccctgctgc cgggtatcag taatgccttc cgccacaaca
      721 ccctcaacgg taaaaggtgt ttttgccgga tctccgccgg tcgcaaacgg ctgatatttt
      781 gatccgaggt aaccggcttc ggaaaaacgt ccctgaggtt ttgtgagtgc gacatacgga
      841 ggaatcagtc cctcataacc ggcgccgtat ccttttttga aagaaagaac agcgccgacc
      901 gacggattga caattccgtc aaccggcttg gtgccggtca taaccatata ggaagcaatt
      961 tcgtgtgcat tgctgccatg cgtcattccg cgaagcaatg aatatttatc cgccatcttc
     1021 gccaataacg gaagtgtctg accgatccgt ataccgctta cattggtatc aatcggcttg
     1081 tcgaattttc cgcaataatc gtagccggct tccggcttcg gatcaaacga gtccagatga
     1141 ctggggccgc cccacagcca cacctgaatt acggacttcg ctttggcttt cagtggattt
     1201 attgcgccgg cggctttgag agtgaacaag tcggaaagca tcagtccggc cgcaccgaac
     1261 atcccgagct gcagggcgtt gcgccgtgta attgccagag acggatctgt tttcgtgttc
     1321 atagcgttct cctttatcag tgcttaagga taaactcact ggtgttgatc agcgcccatg
     1381 ctaaatcata aaagccgttt tcagaccggt ccgacgaaga aatataatcc attgcaattt
     1441 tctgctcttc cggcgacggc atgcgggaaa ggatttccag ataaagcagg tttacgcgat
     1501 ccttgcttcc gggcgccagc gtcatcagtt tacggagccg gggccctttc ataactttat
     1561 tctggatatg cgaagaattc agcaggtgca acgtttggaa aacggacacg ttattattcc
     1621 gatccgactc aagagatgaa tcccggcccg gtcttccgaa catttgtaaa aacgaggatt
     1681 tgatactgcc gtccgccaaa gtaatcgtcc gccggctcga aggaataaat gtaaacggct
     1741 caggaatcgc actggaatac tcttcacctg tcccggtaat ctgacaaatc gcatcaatca
     1801 gcacttcggc atccagccgg cgcgcgcgat aatgagaaaa gccggcttca tcagccaggt
     1861 ttccggacgt tggcagcgaa gaaagctgat aggtcgctga attaagaatc aaccggtaaa
     1921 tatgcctgag atcatatcgg ctctcaacca gttctttttc aagataagcc agcaattccg
     1981 gactccatgc cggattatcc gggcggatgt catccgcttc gtgaatgatg ccccgcccca
     2041 tcatccaaaa ccagatgcgg ttgacaatgg ccttggcaaa ccatggatta tccggagcgg
     2101 tcagccagtc agcaaatgca aggcggggat cgtcgaaatg ccccagcttt aacggacttc
     2161 caccgatcgg tgtcggcaga acaggtgttt ttgtagccgg attaagcagc gttttttccg
     2221 gatcggaaaa gacaatttcc tctttccatt catcggtctt tttatacgcc accttggcaa
     2281 aaaaggcatc catacccagc agttgatctt ccgtccaacc ggatttttcg agccgaacgc
     2341 ccataaaaat cagcgcaact gtgtcgaaca tcttgcgcgg cgtccgctcc tggaacggcc
     2401 ggtaaaagtt ggccggtgcc tcacggaaat tgcttccgct tgccgtgagc agagcgcggg
     2461 cgaactgatc ataaggcatg ttacggcgaa acgcatcacg aagccagatc tgataggcct
     2521 gcaccgcatt cggccaaagg ttgctcgtaa actcggattt aattcttagc agatcgcccc
     2581 acttcaacgt ccagtaatcg gcgaactccg gacgttcaaa cagctcttca atcagttcgg
     2641 aacgcttgtt tggattgcgg ctggacagaa acttgcgaac ctcatccgcc gtcggcagtg
     2701 taccgatgac atcgaaataa acgcggcgaa taaatacagc gtcagaacaa agctcggaag
     2761 gcggaaagcc ggtttcagat tgtttcgcaa aaaccagttt atcgatctgg ttttgcggcg
     2821 aaacggcttg cgaaccgaac ggtacaagaa caacaaccaa cagtaaaaag gctctcacca
     2881 tatgtttcat agtaccctct ggttacaacc cgcgcacata gaacgcccgc cgactgaaaa
     2941 aaattgtaaa acagcaaaaa aatacaccaa aaaacgattc tttgatataa aaaaaccgct
     3001 ccggaaccgg agcggttttc agttttgatg ccgggaaacg gctacccctg caaggccatc
     3061 aaaaactcag cattactctt ggtttttttc aaccggttaa tcagcaattc catggcttcg
     3121 acagccggaa catccttgag tgccttgcgc aatgtccata tccgcgccag ctcttcttta
     3181 tggagcagaa gctcttcctt acgcgtaccg ctcttctcga tgttgatcgc cgggaaaatg
     3241 cgtttaccga ccagatcacg atccagcgcc agctccatat tgccggtacc tttgaattct
     3301 tcaaagataa cttcgtccat acgactgcca gtatcaacca gcgcagtcga aataatcgtc
     3361 agactgcccc cgccttcaat attgcgggct gcaccgaaaa accgcttcgg tttatgcagt
     3421 gcattggcgt ccacaccccc ggaaagaatc tttccgctgt gcggctgaat cgtattataa
     3481 gcgcgcgcca gacgggtaat actgtcgagc agaatgataa cgtcttcgcc gttctcgacg
     3541 cggcgtttag ccatttcaat tacaatctcg gcgacctgca catgacgctc cggatgctca
     3601 tcgaaggttg aagaaagcac ctcggccttt gtcgtgcgct gcatatccgt cacttcttca
     3661 ggacgctcat caatcagcag aatgatcagt ttagcatccg gattgttcgc cgaaatgctg
     3721 ttggcgattt tctgcaaaag caccgttttt cccgtgcgcg gcggtgcaac aatcagaccg
     3781 cgctgtcctt tgccgatcgg cgtcgccaga tccatcacac gcatcgaaat ttcatcggcg
     3841 gtagtttcca ggccaaggcg ttcatccggg aaaaccggcg taagattttc gaacggtatg
     3901 cgggtaaggt tcttttccgg agcattgttg ttaaccttat ccactgacag caacgcgaaa
     3961 aaacgttcct tcggacgcgg agagcggatc gggccttcaa tcaggtcgcc ggtacgcaga
     4021 ccgaaacggc ggatctgcga cggagacaca taaatatctt cggggtttgg cagataattg
     4081 ttttccgacg aacgcagaaa accgaacttg tccggaagca cttcaagaat accgcggcag
     4141 agcgcttcgt taccccagcg gatctgggtt ttcagaattt caaaaatcag ctcgtgcttg
     4201 cgcaggccgg ccggatcttc aatcgaataa ctctcagcca gttttttaag ttccggaatt
     4261 tccgtcacct gaagttcaaa aagtttcaac gtcggcattt tttttgcggg aacgtccgat
     4321 acgcgttcct gctcgtcgcc gttgccgccg ccgttaccgt tgccgttgcc gccccggttt
     4381 ccgttgccgt cattcatgtt gcgcccgtga tgacggggat tgttgcgatt gttattattc
     4441 tgccgacgat tattatcctg acgagaaccg ttattctgac gtgatccgcg atcatcctgc
     4501 cggtgtccat gatcatgacg caacccgcgg tcatcctgcc ggtgtccacg atcatcctgc
     4561 cggcgatcat cgtcctggcg cagcccgcgg tcatccaccc ggtggtcgtc atcctgccgg
     4621 cgatccatat cgtctgtgtt ttctgcctga gggataaacc gttcgacctc cgttgccggc
     4681 accggctcca ccacaggagc ctcagctggc ggtttcggag ctgcaacctt ggcagtccgc
     4741 ggggcgcgtg gagtttttac cggtttcgcg acattttcct gttcttcact catagatatt
     4801 cctcttctca cacctgatct tttcaacaac actcattatc cggctgcgca acatctccgg
     4861 cgtcccgccg ttttcgacca caaaatccgc cctggccgct ttttcagatt gcggcatctg
     4921 cgccgcaatc cgtttgcgcg ccgtttcttt cagcattccg cgcttttcga gccgctggag
     4981 aacctggtct tgcggggccg tcacacaaat cacggcatcc cagcccttcg tccagcccgc
     5041 ttcaaacaga agcggcacca gtaccgcggc atcctcctgt gcctcacggc acgccgctat
     5101 ccattcctgc ccggctgcaa tcaccgccgg atgcaccagc cggttgagcg cttcacgcgc
     5161 tgtcggatca tcgaaaactt tttttccgag ttttacccgg tcaatctctc cgtccgccgc
     5221 cagaatttca tcgccgaaat agtcaaccac cgcagcgtat acagaacgtc cctttcccat
     5281 cagctcatgc gccagaaaat cactatccag caccttaaag ccttctgagg ataaaatgcg
     5341 gcctgtttcg gtttttccgc aggcaatacc tccggtaatg cccagaataa tcgattgagg
     5401 tgaattcata aattaggaag gacagactgg aaaccggttg attacaaaac gttcggtgaa
     5461 aatttgaaaa acgggataag gcaccggaat gcgcagaata gttactgcaa cccgttggaa
     5521 tgtcaacccc gtttactgag catcaagata attctgcagg atgatctgcg ccgccacctt
     5581 atccaccaca cccttgcgcc gtttcccgtt gagccccgcc tcgcgcaaag cgttatgcgc
     5641 actcaccgta ctcatccgtt catcccattc aaaaaccggc acatcggttc gaagtttcag
     5701 cttctcaata aaattccgga ccttctccgt tgcagagccg tacgaaccgt ccatattgcg
     5761 cggcaggccg acaacaatct tttcaacctg atgttcggca atcaaagcga tgatttgatc
     5821 caaaccggcg ttcgtactgt ccagaacgca aagcgacgaa gccagaaact ccgtcggatc
     5881 gctcagtgca acaccgatcc gcacatcacc gtggtctata ccaagcacgc gtcccattat
     5941 ttataatgct ccgtttcaat gcggtgtcgt tcgagcggtt tggttttctc aagccccttc
     6001 tcgatcgcgt gccgccctat cggttccagc gtcagaatcg ccgccacaac cagcagcgta
     6061 accgtccatc cgatcaatgc cgtaacgtaa cttccttttt gaaacagaac cgtcaacggc
     6121 agcacgatga ccagaaccgc cgctgccgga accgccagcg gaagccacca tgcttgaaat
     6181 cccaagaacg gcagcagtgc cagcgctgcg ataatgatca tcagaccgga ggaagtcagc
     6241 agaacgccgg tcgtataaaa agcgccgatg gcacccagac tggtgctgac acggctcaga
     6301 gccagcgcac cgaacatcac acagagtgca acaaccagat aggtttcatt cggagtcgcc
     6361 ctgccgaaga aatgcagcag caacccggaa aattcccgca gccatgtctc catgatttgc
     6421 ctccctgtta aagcaagtga tcgtaagatc cccgctcccg caaccgggcc agcatcttct
     6481 tgatctcctg cgccttttcc ttcgggcaga ccagcgtcac accatcggcc tgcacaacaa
     6541 tcagattctc cgcaccgatc accgccgtca gacggtcttt ggaataaata atattattct
     6601 tcgaatcaat ctgttcacag gttccgatca gcgtattacc gtccgcatcc tgcggaaaat
     6661 ggctttccag cgcaggccac gacccgacat cgtcccacgc aaaggttccg caagccatca
     6721 caatgttatc cgccttttcc atcagcgcat aatctaccga aatttttccg agtgccggat
     6781 agattttttc cagcccgacc gaaatttcgc cgcgtttcgc gtaagtggtc agctcatcca
     6841 tcagcgtctt catttccgga caatgcattg caaaagcctt tgccagcgtc ggaaccgacc
     6901 agataaacat gcccgagttc cagaaaaacc tgccgctcgc cagatattcc tgcgccttcg
     6961 ctaatgccgg cttctcaaca aaccgaaccg ccttcttaaa ctgaacacct ccggcctttg
     7021 caaaatccgc acccgactca atataaccga agcctgtgct cgggaaagcc ggctgaatac
     7081 cgatagtcac cagaatttca ttttccccgg caagagccat accgccgcgc agtgtcgctt
     7141 taaaaatatc cagatcaccc atcacttgat cggcggtcag caccgcgaaa acagccttcg
     7201 gatcgcgtgc gccaaccagc gcgccgccgc aggccaccgc agccgccgtg tcgcggccga
     7261 tcggttcgcc gacgatattt tccggcggaa gcatcggagc cgccgcgcgc gtcgcttcca
     7321 ccagatcggc attcgtaacc acaaaaacat tttccggcgg aatcaacccg tcgagccgtt
     7381 ccaccgcctg cgcaatcagc gccttatcgc ccgccagcgc cagcaactgc ttcggatgct
     7441 tcgacgtact cagcggccag aaccgctcgc ccttcccgcc cgccataatc actgcaaaac
     7501 gtcctgacat ttaaatctcc taaaaaattc cacatgttaa gggcggcttt tttgtccata
     7561 attaacttgc cgcacaattc cttccaaaca ttggaacgtt tctaccggat tcttccagag
     7621 tttggaagcg tgactccggc attattaacg actgatttcg cgtcgccgat gcttcattgc
     7681 gtcgagttat ttgagctttt ccagcttggc gagcagaacg tcccgcaaac gcggtttgtc
     7741 gtcgggatag agcgagatga gttttttgcc ctgctgctga tagagctgct gttttttgag
     7801 catgccgatt ttgtaatcgg cggtgtccat gccccagtat tcaacataga catcgaattc
     7861 cggcagatag aagtcgggcc ggatggcgtg tccgctgagg atgcgaaacc gttcgtcgta
     7921 gcggtattga atgccttctg tcgccaacac ttcacaaatc cgccgttcac cgtcagactg
     7981 cacaatcgtt ccgtctttgg cgcggatggt tttctgaagt tccactttgg tttcaaagtt
     8041 gcgccggtcg agaaatacct cgtcgaaaca atggtcgcag aaaggtctct gaaaagcttt
     8101 ctgcgaacgg gcgtattcgt ctgatgccag ctttacgccg caacggcggc agcagtgaac
     8161 aacctgttcc tcggcgatca gcaccgcttc acggatcgtt ttgccggcgg ccagcacact
     8221 gcgtttgaca tagtcttttt cgaccgggct tttgtagagg tcgcgcagat ccggcagaac
     8281 gtccttagcg tgtgttccga aagcaccgag cgccttggcg gcatactgcc tcacctgcgg
     8341 atgaacatcc gacagcaacg gctgaagtgt tttgacggca gactccggcg caacaatccc
     8401 cgccagttta ccaagtgcgg aagcggccag ccgccgaacc agcggcgatt cagaacgggt
     8461 taattctgcc aactcagcaa atgaagcgga actggcactt tcgcccagct ctttggcccg
     8521 cgcagccagt tttctttcat tgctcaaatt catgactgat tttgcatctc tgatgctcca
     8581 tcgcgtccga agtaagttaa acaacacaac aaaggacgct aaacggcttt aacagccctc
     8641 actatttcgg cgacgaaggc ggcggcatcg gcgcggcctt cgggcgtgtg cgggttgttg
     8701 taaaacttat tcacgatggc actgccgaca acgacggcgt cagcgaacga tgcggcctca
     8761 gcggcttgcg caggcgtgcc gataccgaag ccgagcgcaa ccggcagtct ggtgtgcgtc
     8821 ttgatttttc cgaccagtcc gcgcgcacct tcagcgattg agtcgcgaac tccggtgacg
     8881 ccttcgcgcg agacgcaata aacaaaaccg ttggcgtctt tgacgatctg tttcatccgt
     8941 tcttccggag tcgtcggcgt aatcagcgaa atgttgttca ggttcgcacc ggaaataaat
     9001 ttacggtacg gccccgcttc ttcgcgcggc atgtcgagca ccagaaagcc gtcaatgcct
     9061 gccgtcgaag cctcgttcat ggctttttca aatccgcggg cataaagcgt gttgaggtag
     9121 gcgtagaaga tcatcggaat ctgcgagcgc tcgcggattt tccgaaccat ttccatgaca
     9181 ccgtcaaagg tggcaccggc ggccagcgcg cgagtggcgg agtcctggtt gacacgtccg
     9241 tcggccagcg gatcggaaaa cggcaggccg agttcgacga gatccactcc ggcctcttcg
     9301 aggcgcagga cgatatctac cgtgtcatca agcgtcgggt cgccggcgcc gatgtaggcg
     9361 ataaatcctt ttttgccctg ctttttaagg tcagcgaatt tttgatcaac gcgtgtcttt
     9421 ttcatttctt ttccagaaac ttgcggatga gaagcgcgcg tttgatatca cgttcttccg
     9481 gactcagtgc agatccggct tctttctgaa ggtgcgccgg atgaatctga ataaaaagcc
     9541 ggtcaatctc ccggcgcacc attttcggca gcaggccgaa gtccacgccc atccgcagcg
     9601 ccgaaagcag gtcgagagct tcgccggtgg tcatcaatgc agcgttagcg aggatgccgt
     9661 aggcgcggcc gacgtgatcc tgcacacgga tttcctgcgt gctcatcagc cggatccgcg
     9721 catttttttc atgctcaatt acttcaagaa cgatctgttc gaggttggag ataatttccg
     9781 gttccgggcg gccgagtgtg atctggttgg aaatctgaaa catatggccg gatgcctcgg
     9841 agccttcgcc ccacaacccg cgcacggtca gaccgatttt ggaaaccgca ttgatcaccg
     9901 gcttgatttc ttccgtcaga acgagccccg gcagatggag catgacgctg gcgcgcaggc
     9961 cggttccgac gttcgaagga caggccgtga gataccccag tttgctcgaa aaggcgtagg
    10021 aaagactctg ctcaagcgca ttgtccagtt catcggccac ttcccaggct ttttgaagat
    10081 tcattcccga ctgaagcgac tgcaaccgga tatggtcttc ttcgttcacc atcaccgcgc
    10141 ggcattcgtc ttcgttaaca aatacgccgc tgcccgtctg gcggtcggcc agctcgcggc
    10201 tgatcagatg gcgttcgaaa agcagttcgc ggtcaagcgc actcagctcg tccatccgcc
    10261 agcgcaaaaa gcttttgctg tccggtacgc cgtcgaatgc cagaaggact tcgttccaaa
    10321 cgcggttacg aacgtccttt gaggcccagc cgggaaatgt ggtatcgcgc agattgcggg
    10381 caagccggac gcggctgctg ataaccggcc cttcgtccgg cccggtttca agccagccgc
    10441 cgtgacgctg taaaagtccg tcgagggtca tttcgctccc ccttcgcccg gtgtgccgtc
    10501 ttcgcggcat tttttgatct ggtcgcgcag tttggctgct ttttcaaaat cctcgtgggc
    10561 aatcgccgct tcgagttctt tctgaagccg ggcgattttc gatttcatcc gcgtctgcac
    10621 accttcgcgc gccggaatct tgccgaaatg ctgcccgctg tggtgcatcg ctttgattgc
    10681 catcgtcagt tccgccataa aggttttata acagtccggg cagccgagac gtccggtttt
    10741 acggaattcc tcgcgcgcca tgccgcagcg cggacaggcc agactcagct cgctggcaat
    10801 attcttttgc ggcccgctca atcccatcag aatatcggta atagaaatcg gactgttgag
    10861 gtcgatcccc tgtgctttgg cgtgttcctc acagagatga acctctttca tctctccgtt
    10921 gaccacctgc gtcaggtgaa cgacagcttc tttgtcgcag agatcacatt tcacagaagc
    10981 ccctttattc aatcggtgtg ccgttattgt tgacgaagtc gcgataggct ttgatcagct
    11041 tgttcgtggc cgggccgggc cggccggttc cgatgatacg cttatcgagt tcgacaaccg
    11101 cgataacttc cgccgccgtt ccggtcagga aaacctcatc ggcagtccag agctcgtaac
    11161 gcgtcataac ggtttcctga acatccatac cgagttcacg gcccagctgc atcaccttgt
    11221 cccgggtaac gccttccagc gcgccgcacc aggtaggcgg tgtcatcagt ttattgcctt
    11281 tgatggcgaa aacgttatcg ccggaggcct ccgcaataaa cccctgagag ttgagcagaa
    11341 gacattccat cacaccggcg ttgatcgctt cgatcttggc catgatgtta ttgagatagt
    11401 tcaggctctt aacgcgcgga ttgaccgcct cggccatgtt gcggatggtg ccggccgtga
    11461 tgatcttcat gcccttttca taaagttcgg ccggatagag ctgaatatcc gccgcaatga
    11521 taatgacctg agcctgttta cactgaaagg gattcagccc cagcgaaccg actccgcgcg
    11581 tgacaacgtg gcgcatatag ccgtttttta aaccgttggc tttgcaggtt tcaatcgtcg
    11641 cctttttgag ctcatctttg gtcataccga catttaaatt aattactttt gcggaatcga
    11701 gcagacgatc catgtgttca tcgagaaatg caacgcgtcc gttatataca cgggtccctt
    11761 caaaaacgcc gtcgccgtac agcaggccgt gatcaaaaac cgaaacaacc gcatcctttt
    11821 catcaaccag tttgccgttt aaaaaaattt tcatagtcag tccctattct tccagttttc
    11881 cgtcttttaa aacgagttcg cgctgacagc ggcgggccac ttcttcattg tgcgtcacca
    11941 taatcaacgt gcgctgtgca gattgcgcta aatcaaagag gttgttcaat atattttcgc
    12001 cggtatgcga atccagattg ccggtcggct catcggccag aatcaggtcc ggatcgttca
    12061 tcagcgcgcg ggcgatcgcc acccgctgct gctcgccgcc ggaaagctcc atcgggcggt
    12121 gatccatccg cccggccagc ccgacctgtt cgagcagata ttccgcccgc gccagcacct
    12181 cgcgcccgcc gcccgccgcc atcgacggca gaatcacatt ctgccggaca tccagttcgg
    12241 gcagcagatg gtagctctga aaaatgaagc cgcagcgctt cgagcggaaa gccgcccgct
    12301 tggccggatt cattttcacc aggctggtct cttcgaaaaa aatatcgccg gaatccggcc
    12361 ggtcaagccc gcccagaata tgcatcagcg tgcttttacc ggcgccgctg gcgcccataa
    12421 tcgaaaccgt ctcgccgcgg ttgatcgtca gcgtcacgcc atgcagcacc tccaccgcgc
    12481 gtttgccgat gtgataggtc ttgtgaacct cttccgctct caaaatcgtt ttactcatcg
    12541 ctactgctcc tgcgccttgg ccgcctgctc cgcagccagc ttttcatatt tcgccagccg
    12601 gctcagcgcc ttgtcagaaa tcggcttgtc cggcgagatt ttcttccgga aagcgatcag
    12661 ccgcttctgg gcctcggcgg ctttggcgta ctgcccgttg ttctcatagg ctacggccag
    12721 cgtatcgaga ttgaaaatct gctcttcttt agaaaccgac aactcggcca gccggatcgc
    12781 ttccggataa ttccgcaacg acagatcgtc acacgtcgcc agaaaccagg catacccgtt
    12841 taatgccgtc gcatgatcag gctgaatttc cagtgctttc cgataataga gaaccgcctg
    12901 ttgcagattt ttttctacac ccttaccttt gctatagcac aacgctaaat tacacaatgc
    12961 ggtgacattc ccctgatctg ccgccatccg gtaccatttc aaagcctcct gcatatcttt
    13021 ttctacgccc aaccccgtct cgcagaagta acccaacgac agctgcgcgt catcattctg
    13081 ttgtgccgca gccttccgat accaatcaaa ggcttttaca aggtctttct ccacaccccg
    13141 tccattctga taacaccatc cgagtgcata ttgtcccttg gcataaccca tttctgccgc
    13201 atggcgataa tgttcgatca tttctgcgtc gcggcccaac tgctccaaac aataaccggc
    13261 gctgtgaatg tcatatgcag aggacggatc taagcggcaa gcttcattgt gtttttccag
    13321 cgcctcttca taacgaccca tacagcgcaa caccccgccc cagtcactcc agcagtcctc
    13381 gcatgagggc tctaactgaa ccgtccgcgc aagaacagca cacgcctcat cgcacct
//
LOCUS       DAOPZO010000038        13427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_5061_length_13427_cov_9.094900, whole genome shotgun sequence.
ACCESSION   DAOPZO010000038 DAOPZO010000000
VERSION     DAOPZO010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 13427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13427
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5061_length_13427_cov_9.094900"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="PKI68_03965"
     CDS             complement(<1..822)
                     /locus_tag="PKI68_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNX52879.1"
                     /translation="MLVIGISFAVITLISSTYRFHRMLQLSEEALTTVEDCNDFFFVQ
                     VTRYLSKINRVSSGFGVFVVQFVAETPDKRRVQEEMLGVLKKIVRDAHDKVCLFRDDC
                     VAAIIDTDESTVPEMAQRITLDLKKNIGSVPSVKTFRAGVSSFPAHGLSTQQIIDTAT
                     QALETGSFENQLPFCLAPWPADNENRKPEGEAVALAHEDKRSAIDSLTGVLSSAAVGS
                     YMRKYLADIRQRKQSATLLCVGINRIDQIIKLYGEPAADDVIAGVGKILQHVTRDS"
     gene            complement(922..1437)
                     /gene="pal"
                     /locus_tag="PKI68_03970"
     CDS             complement(922..1437)
                     /gene="pal"
                     /locus_tag="PKI68_03970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HNX52880.1"
                     /translation="MKKSASFVIGMSLLLAVSTLTGCKSLKKNPAGVYGSDGLYGNGA
                     NGSLGMGDRFTGGTEHPGMFAPVYFDYDSAQVADSERSKIETVAQHLKQNDSVAVIIE
                     GNCDERGSNEYNLTLGERRAQAVRAYLAGLGIGADRIQTKSYGEEKPVNPGHDEAAWA
                     ANRRGEFVLYY"
     gene            complement(1466..2662)
                     /locus_tag="PKI68_03975"
     CDS             complement(1466..2662)
                     /locus_tag="PKI68_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52881.1"
                     /translation="MKKLILFTLSIVISASFSQAQVRVVKSADRKITIDLSGLRTAGD
                     SASQTFSQTLEKDLRLSGWFDPVRGGGELRLNGTAAPSGGELKAGGQVFRLNDQAMLF
                     SKNYNGQPRAIAHRMADDIVEAVTGHKGFASMRIVLVGNRTGKKELYLCDGDGGGLRQ
                     LTGEGHIAIAPDWGPDGNTIIYTSYMRNFPDICRIDLNKSRREALASYGGLNTGSSIS
                     PDGKDLALILSKDGNPELYIKNIRSGKLTRLTNTPRAAEASPDWSPDGSRLVYASDQS
                     GTGKPQLYIISREGGTPRRISSRGTENIAPDWGPNGLIACASRTGGHYAIAVIQPSSG
                     QTSYLVSDGADYEDPSWAPDGRHIVAARSVNYQSSIYLLDTVSDRPVALLQGSGGWYS
                     PACSPQ"
     gene            complement(2773..3648)
                     /locus_tag="PKI68_03980"
     CDS             complement(2773..3648)
                     /locus_tag="PKI68_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNX52882.1"
                     /translation="MKNSLAKKTFGRVSIVHGVVLLTAFFLPVLRGCAPKPKEVITFV
                     DLAGAPAPASAPEPESDESPAPELKPESKPVPKPEPKPEKKTEKKLTPRPAVTNTPPK
                     KVETNAPPKKVEPKKAAATNAPPKAKTLEERLKEVRQGGRPVTAPSAKTSTSKRPQLD
                     FSGIKSALNSSSAGSGSASSFGTGSGTAASGTGSGSGGGMYSPFAGYYDSIKQRMYSV
                     WPQPSGAPKGIPVTASIRVERDGTVSFKSISRRSGNAQLDQSVQNALNATTRLPPPPA
                     DLPDLNITVEFVLSD"
     gene            complement(3662..4075)
                     /locus_tag="PKI68_03985"
     CDS             complement(3662..4075)
                     /locus_tag="PKI68_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX52883.1"
                     /translation="MRRRRRSSLIALRESNEINITSLMDIIVLLLFAFIITMPIIEQN
                     LPVNLPNAKAGAMDLTKKHHTIELSIKDGLTLDSAPMSLQRLGEKMKVIGTTEPEVPV
                     YVRADEKLDYGKVVEVIQVLKDAKIKTMGLVTQGE"
     gene            complement(4087..4836)
                     /locus_tag="PKI68_03990"
     CDS             complement(4087..4836)
                     /locus_tag="PKI68_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNX52884.1"
                     /translation="MNGLLLPLMTANIGLAFRQSELNGKVIVLVLVFISIGAWTVMLT
                     KFAQLRAARLASNRFLKTFRGESYVTNLFIKRHHAPDTPLSAMYDAGCQALRAELKAD
                     GIERPEDLFAGKTDTAETRKLDSSQMDAIRSAVDCALADKALELESLMGLLGTVVSVG
                     PFLGLLGTVWGVMGTFSGMATAGSATLSAVAPGISGALLCTVAGLLVALPATIGYTLL
                     STRIRYLAVQMDNFAQEFTGRIQQEFMLNRK"
     gene            4952..6112
                     /locus_tag="PKI68_03995"
     CDS             4952..6112
                     /locus_tag="PKI68_03995"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNX52885.1"
                     /translation="MKKILSAIPALLPLLISLLLTGAAPAHQPEVRAIWVTRFDYKTP
                     ADVDAVVANCVRTGFTDIFFQIRGNGTVFYPSRTEPWAFELTGDNASFTGRNPGWDPL
                     LVAATSAKKRGIRLHAYMNVLPGWRGLQEPPRSAGQNWTAHPEWFMVDALGQRMRPTS
                     GWYAFLNPALPEVRQHLTQLADELARYDIAGLHLDYIRFPHDYKDVAPQLYPRASAAE
                     IAKRSDFSYDRFSSAQVQQRYKGNWNAFRRDAVSQVVAELNKTFKTRRGPQCIVSSSV
                     LADFDKGYGTAFQDSRRWAGEQMVDWLVPMNYNAPLFDERLGKIRSSLGRSATASQLV
                     VGIDCKADLSEIRREIASVKKAGCRGYALFAYSYLFENHRPTAKGRALASVL"
     gene            complement(6190..8199)
                     /locus_tag="PKI68_04000"
     CDS             complement(6190..8199)
                     /locus_tag="PKI68_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52886.1"
                     /translation="MSNKKSLGLVLVLLGFILLALTGMRTISSPEIFTHIALGQAGSA
                     NADTLSYTMAGQQWINMNPLYNSLVYLLWSLGGAPLVTVVHILAVLAAFIMMFRFSKE
                     WGGPLSQGLALLLCARLIFPVFNPDPTVFFMLFTALFLTCLYRIKNFNLLAALLLIFQ
                     VLWTNMHPSFLFGPVLILFFAFENWQGTRQPSRSAMITPLTAKLFGLSAVALAATLIN
                     PNLINLHRHIFANWVLLTGTDGLEWISLFSCFFPQGYISNLTVFALILGAGGLITLQK
                     RLPAMLTMLALVGAFLTVRSIGSLGLFAFLAFPFMVLSFNAVSEYLVRTLTTMFKASE
                     GMLHKLLSAVALILMISSIVSLVTNRAYINIGSASHFGLGVEEGAFPVAAARVLSRSD
                     FPKNIMNIAHDGGYIALQNPGRKIFCDTRISFYGTGFNQTLNRALLGQPEAWKTILSN
                     WNPHAIVLNGCWPDAGDLVARLIASTAWKLTYFDGSTVILVRDLPEYAALIHDSAIQK
                     DGLAVLEKSRRDYLSQNKGFFKSGNPSRLIGAGGIYLALRRPLEADAVYTALIKNSPL
                     MAGAWMGLGQSLVYQRQLSKGIEYMEKAVGITPHSGRIWMSLFQAYRIKGDERKMNDA
                     ADKLNKFYKADSATVEQQALKEQKKPAEKPKQKSDLDVQMPAELK"
     gene            complement(8282..10771)
                     /gene="mutS"
                     /locus_tag="PKI68_04005"
     CDS             complement(8282..10771)
                     /gene="mutS"
                     /locus_tag="PKI68_04005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNX52887.1"
                     /translation="MAEKSTPMMDQYRRIRRELPENTLLFFRLGDFYEMFMEDAKEAA
                     RILDITLTRRHDIPMCGIPYHAAEGYLAQLIEAGKKVAICEQVEDPAAAKGIVKREIT
                     RVVTPGTVLDETALDRTKNNYLAGLVRENGRFGLAMLDLSTGAFWIEESASAGALSGN
                     LARYAPQECIVAEAAADDAEILAVVGRTLKTVGEDWTFEFKSAEDVLLRHFKVHSLKG
                     FGCENSPLGLRAAGAVLYYVNTGLRRNLAHVRRIQVKNPDDYLLLDETTVMNLELVAP
                     LNPARQGPKSTLLSVLDTTRTAMGARMLRDWIVRPLRDLSEINARLDAVEAFTANRTL
                     LAHVRELLGEMRDLERIMSRLGSGGGNARDLRAVGESLDQLPKLKELLAASGNFQALE
                     KSLTELPGIGKMINEAVDDEPAVSTKDGGMIRRGFSAELDELRDAAGKGKQWLAEFQT
                     AEQERTGIKSLKVRFNQVFGYFIEITNSNLSMVPATYTRKQTMTNAERFITPELKEYE
                     NKILGAQERAVALEFELFTQIRDEVVKHIDEIQRNARAAAEVDVLASFAENALTRRYV
                     RPAMSAGDELIICDGRHAVVEQMPDAERFVPNDTTLNCTDHQLIVITGPNMAGKSTYI
                     RQVALITIMAHIGSFVPASEARIGLVDRVFTRVGASDDLARGRSTFMVEMQETANILN
                     NATPKSLIVLDEIGRGTSTFDGISIAWSVAEFLCTNPAVRAKTLFATHYHELTDLSLT
                     MPGVQNANVLVKERGDQITFLRKIVPGAADKSYGIHVARLAGLPASVLERAAEILHNL
                     EEGEFEAEGTPKLAQHRTRRKKTDDSQMNLF"
     gene            complement(10827..>13427)
                     /gene="carB"
                     /locus_tag="PKI68_04010"
     CDS             complement(10827..>13427)
                     /gene="carB"
                     /locus_tag="PKI68_04010"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNX52888.1"
                     /translation="LEESVYGWKEFEMEVMRDKKNNAVIICSIENMDPMGIHTGDSIT
                     VAPTQTLTDREYQAMRNASIKIMRAIGVETGGSNVQFCIHPETGRMAVIEMNPRVSRS
                     SALASKATGFPIAKLAAKLAVGYTLDELPNDITKETPACFEPSIDYCVVKMPRFAFEK
                     FPSADAHLGVSMKSVGETMAIGRNFKEALQKAFRSLEIGVDGLDLKAEAKVPLARLDE
                     WLTRTKTERCIAIKVALKQGYSVQKIAEITKIDPWFIDNILQIVEMEREILDAPKDKP
                     LDADLLRKIKLNGFSDAQIAVLRGQDPLAFREERKKAGVIPVYRLVDTCAGEFEAATP
                     YFYSSYGDLNEIRKTGRKSIIVLGSGPNRIGQGIEFDYSCVHTVKALREMGYEAIMVN
                     SNPETVSTDYDTSDKLYFEPLTFEDVMNIYEQEQPIGMIVQMGGQTPLNLADRLLAAG
                     VPILGTSPKSIAMAEDRELFRQMIIKLGLKQPESGTAKTMDDALKIAHRIGFPVMIRP
                     SFVLGGRAMMVAYDETEFVPFTHAAFNASPGHPVLIDRFLENAIEIDVDLVCDGKDVY
                     VGGVMQHVEAAGIHSGDSACSIPPYSLDPKIVLEIEAACTRMALELNVKGLMNAQMAV
                     KGSDIYVIEVNPRASRTVPYVSKATGVPMAKIATEIAVGKTLKELGLVGFRPKLSWYA
                     VKEAVFPFNRFAGVDPILGPEMKSTGEVMGIDKTFEMAYWKSQIAAGQMLPAKGCVFL
                     SATDRDKGWMVEIGKALVDLGFTLTATEGTAKALCAAGLKVTETCKLASGQHPNIIDF
                     MQDGKIAMVINTPSGPVSRMDEIKIRSECILRNIPIVTTESGARATLGAIKAIDGNDW
                     TVRPIQDYHA"
BASE COUNT         2661 a         3722 c         3970 g         3074 t
ORIGIN      
        1 gctgtcgcgg gtgacgtgct ggagtatttt cccgactccg gcgattacgt cgtcagctgc
       61 cggttcgccg tacagtttga tgatctggtc aatgcggttt attccgacgc agagcagggt
      121 cgcggactgt tttctctggc ggatatccgc aaggtattta cgcatgtagg agccgaccgc
      181 cgcagaactt agtacgccgg tgagcgaatc aatggccgag cgcttatctt cgtgagcaag
      241 cgcgacggct tctccttccg gtttccggtt ttcattgtcg gccggccacg gcgccaggca
      301 gaacgggagc tgattttcaa agctgccggt ttcaagtgct tgcgttgcgg tgtcgatgat
      361 ctgctgagtg ctgagaccgt gcgccggaaa actgctgact ccggctcgaa aagtttttac
      421 ggaaggtacg cttccgatgt tttttttcag atccagcgta atgcgttgtg ccatctcagg
      481 cacggtggac tcgtccgtgt cgatgatggc ggctacgcaa tcgtcgcgga acaggcagac
      541 tttgtcgtgg gcgtcccgga cgattttttt tagaacgccg agcatttctt cctgaacacg
      601 ccgtttgtcc ggtgtttcgg caacgaactg gacaacaaag acgccgaagc cggacgatac
      661 cctgttgatt ttgcttagat agcgggtgac ctgaacgaag aagaaatcgt tacagtcttc
      721 cacggtggtt agagcctctt cgctgagctg gagcatccgg tgaaagcggt aggtgctgct
      781 gattagagtg atgacggcaa acgaaatgcc tattacgagc aacagctgcc cggcctgagc
      841 caagggcagc tgcgctaccg gtgaaaactg tagaatggaa ctcaacatgt aggggtcgct
      901 tcagagaagc gcggcagcgt gctagtaata cagcacgaat tcgccgcgac ggttagccgc
      961 ccaggccgct tcgtcgtgtc ccggattaac cggtttttct tcgccgtagc tcttggtctg
     1021 aatacggtcg gcaccgatgc cgagaccggc gagataggcg cggacggcct gcgcgcggcg
     1081 ttcgcccagc gtgaggttgt attcattgct gccgcgttcg tcacagtttc cttcgataat
     1141 cactgcgaca gagtcattct gtttgaggtg ctgagcaacg gtttcgattt ttgagcgttc
     1201 gctgtcggct acctgtgcgc tgtcatagtc gaaataaacc ggggcgaaca tgccgggatg
     1261 ttcggttccg cctgtgaagc ggtcgcccat tcccagagat ccgttggcac cgttaccgta
     1321 aagtccgtct gatccgtaga cgccggccgg atttttcttc aggcttttgc agccggtgag
     1381 agtggagacg gccagcaaca gagacatacc aataacaaaa gaagcacttt tcttcatgac
     1441 aacgagacct cgcatttact tgtttttact gcggcgagca ggccggtgaa taccagcctc
     1501 cgctaccttg gagtaaagct accggacggt cgcttaccgt gtcaaggagg tagatggacg
     1561 attggtaatt gactgagcgg gccgcgacaa tgtggcgtcc gtccggcgcc catgaagggt
     1621 cttcatagtc agcgccgtca ctgaccagat agctggtctg tccggaagag ggctgaatga
     1681 cggcaatcgc atagtggccg ccggtccggc tggcgcaggc gatcagaccg ttaggtcccc
     1741 agtccggcgc aatgttttca gtcccgcggc tgctgatgcg cctgggtgtg ccgccttctc
     1801 ttgagatgat gtagagctgc ggtttgcctg taccgctctg gtcggaggca taaaccagcc
     1861 ggcttccgtc cggcgaccag tccgggctgg cttcagccgc ccggggggtg tttgtcaggc
     1921 gcgtcagttt tccgctgcgg atatttttga tgtagagctc cggattgccg tctttggaca
     1981 gaatgagggc aaggtctttg ccgtccggcg agatcgatga gccggtattc agtccgccgt
     2041 aactggccag tgcttcgcgc cggctcttat tcaggtcgat ccggcagata tccgggaaat
     2101 tccgcatata cgaggtgtaa ataatggtgt ttccatccgg accccaatcg ggggcaatgg
     2161 cgatatgccc ttcaccggtg agctggcgca ggccgccgcc gtctccgtcg cacagataga
     2221 gttctttttt gcccgtgcgg tttccgacca gcacaatgcg catggaggcg aatcctttat
     2281 gtccggtaac ggcctcgaca atgtcgtctg ccatacgatg ggcgatcgcg cggggctgtc
     2341 cgttatagtt tttggaaaac agcatggctt gatcgttcag ccggaaaacc tggccgccgg
     2401 ctttcagttc gccgccggat ggcgccgctg ttccgttcag ccggagttcg ccgccgccgc
     2461 gcaccggatc aaaccatccg gaaagccgca ggtctttttc cagagtctgg gaaaacgtct
     2521 gtgatgcgga atcacccgct gtgcgcaggc cggataaatc aatggtgatt ttacggtccg
     2581 ctgactttac gacacgaacc tgagcctgac tgaaggaggc agagatcaca atggagagag
     2641 taaacagaat gagttttttc atagctctac tcattagcgg aatctttcgg ccagtgaaag
     2701 ctgttatttc cggcaaccgc tgttaacagg ccggatacgg cagcgttgat gaagtctgct
     2761 tgcgtccacg ttctagtccg acaggacgaa ctctacggtg atgttgagat ccgggagatc
     2821 ggcaggcggc gggggcagcc gggtggttgc gtttaacgca ttctgcaccg attgatcaag
     2881 ctgtgcattt ccggaacggc gggagatgga tttaaatgaa accgttccgt cgcgttccac
     2941 ccggatggag gccgttacgg gaatgccttt cggtgcgccg gacggctgcg gccaaactga
     3001 atacatccgc tgcttaatgc tgtcgtagta gcccgcaaac ggactgtaca tcccgccgcc
     3061 ggaacctgaa cccgttcctg atgccgctgt tcctgagccc gtcccgaagc tggatgctga
     3121 gccggatccg gctgacgagc tgttcagagc cgacttaatt ccggaaaaat caagttgcgg
     3181 acgtttcgac gtacttgttt tggcggacgg tgcggtaacc ggcctgccgc cctgccgtac
     3241 ttctttcaac cgttcttcga gagtttttgc cttgggcgga gcattggtgg cggccgcttt
     3301 tttcggttca acctttttag gcggggcgtt ggtctcgact ttttttggcg gcgtattggt
     3361 gacggccggc ctgggcgtca gtttcttttc agtttttttt tccggtttcg gctcgggttt
     3421 cggcaccggt ttggattccg gtttaagttc cggtgcgggg gactcgtcag attcaggctc
     3481 gggagccgaa gccggagccg gtgcgccggc gaggtcgaca aacgtaatga cttccttcgg
     3541 tttcggtgcg catccgcgca gaaccggcaa aaaaaaagca gtcagcagaa cgactccatg
     3601 cacgatggaa acccgtccga atgttttttt tgccagggag tttttcacgg ttatatgcgg
     3661 attattcgcc ctgagtcacc agtcccatgg tcttgatttt cgcgtctttc aaaacctgta
     3721 taacttccac cactttcccg taatccagtt tctcgtcggc gcgcacatag acgggaacct
     3781 ccggttcggt tgtaccgatc actttcattt tttcgcccag ccgctgcaga gacatcggtg
     3841 cgctgtcgag cgtcaggccg tctttgatcg acagttcaat ggtgtggtgt ttttttgtta
     3901 aatccatcgc gccggctttt gcgttcggca ggttgaccgg caggttctgt tcgatgatgg
     3961 gcatggtgat gataaaggcg aacagcagaa gcacgatgat atccatcagg gaggtgatgt
     4021 taatctcgtt gctttcgcgg agcgcgatca ggctggacct tcttcttctt ctcacgaccg
     4081 gctcctttat ttgcggttca gcatgaattc ctgctgaatg cgccctgtga attcctgtgc
     4141 aaagttatcc atctgcacgg caagatagcg gatgcgggtg gatagcaggg tgtatccgat
     4201 ggttgcgggc agcgccacca gcagtccggc tacggtacac agcagtgcgc cggagatgcc
     4261 gggagccacg gcggaaagcg tggccgatcc ggcggtcgcc attccgctga aggtgcccat
     4321 gacaccccag acggttccca gcagcccgag gaacgggccg acgctgacaa cggtgcccaa
     4381 aagacccata aggctttcca gctccagcgc tttatcggca agcgcgcagt ccaccgcgga
     4441 acgtatggca tccatttgtg agctgtccag tttacgtgtc tcggctgtat ccgtcttgcc
     4501 tgcgaataaa tcctccggac gttcgatgcc gtcggccttc agttccgcac gaagagcctg
     4561 gcatccggca tcatacattg cactgagcgg cgtgtccggt gcatggtgcc gtttaataaa
     4621 gaggttggtc acataggatt ctccgcggaa ggttttcaga aaacggttgc tggccagccg
     4681 tgcggcgcgc agctgtgcga attttgtcag cattaccgtc caggcgccga ttgaaatgaa
     4741 caccagcaca agaacgatca cttttccatt caactcgctt tgacgaaaag ccaggccgat
     4801 gttggccgtc atcaggggga gcagcagtcc attcataatt tgttatctcc atttctacgt
     4861 attcggaaaa attggttcgt attctccaat gtttggaaaa acagtacagt tttttttcca
     4921 aacatcgtaa aaatcaacta tgctccgccg catgaaaaaa atcctgtccg ccatcccggc
     4981 ccttcttcca ttgctgattt cactgctttt aaccggtgcc gcgccggcgc atcagccgga
     5041 ggtccgtgcg atctgggtga cgcgtttcga ctataaaacg ccggctgacg tcgatgcggt
     5101 tgtcgccaac tgcgtccgca ccgggtttac cgacatcttt ttccagatcc gcggcaacgg
     5161 aaccgtgttt tatcccagcc ggacggaacc ctgggcgttt gagctgacag gtgataacgc
     5221 ctcgtttacc ggacggaacc ccggctggga tccgctgctg gtcgcggcga ccagcgctaa
     5281 aaagcgcggg atccgcctgc atgcctacat gaacgttctt cccggctggc ggggcctgca
     5341 ggagccgccg cgctccgccg gccagaactg gacggcgcat ccggagtggt tcatggtcga
     5401 tgcgttaggt cagcgcatgc gtccgacgag cggctggtat gcgtttttaa atccggcgct
     5461 tcccgaagtg cggcagcatc tgacgcagct ggcggacgag ctggcgcgct acgatattgc
     5521 cgggctgcac ctcgactaca tccgctttcc tcacgactat aaagatgtgg caccccagct
     5581 ttatccgcga gcctcggcgg cggagatcgc caaacggtct gatttttcct atgaccggtt
     5641 ttcatccgcg caggttcagc agcggtacaa aggcaactgg aatgcgttcc gccgtgacgc
     5701 tgtttctcag gttgtcgccg aactcaacaa gacctttaaa acccggcgcg gtccgcaatg
     5761 catcgtttcg tccagcgtgc tggcggattt tgacaagggg tacggcactg cgtttcagga
     5821 cagccgccgc tgggccgggg aacagatggt ggactggctg gtgccgatga attacaacgc
     5881 gccgcttttt gatgaacggc ttggtaaaat ccgcagctcg ctgggccgca gcgccacagc
     5941 ctcgcagctg gtggtcggaa ttgactgcaa agccgatctg tccgaaatcc gccgcgagat
     6001 tgcttctgtg aaaaaagcgg gctgccgcgg ctacgcgctg tttgcctatt cctacctttt
     6061 cgagaaccac cgtccgacag cgaaagggcg ggcgctggct tccgttttgt agcccgcttc
     6121 ttcggttctt gatgaacgtt tcagaagcgt ccggtctgag cgtgcgcgaa gcgatccttt
     6181 aggcccgggt tatttcaact cggccggcat ctggacatcc agatcggatt tctgcttcgg
     6241 tttttcagcc ggttttttct gttccttgag agcctgctgt tcaaccgtgg cgctgtctgc
     6301 tttgtagaat ttattcagct tgtcagcggc atcattcatt ttccgttcat cgcctttaat
     6361 gcggtaggcc tgaaagagac tcatccagat gcggccgctg tgaggagtga tgccgaccgc
     6421 tttctccatg tactcaattc ctttggagag ctgtctctga taaaccagac tttgcccgag
     6481 acccatccag gcgccggcca tcaacggact gtttttaatc agggcggtgt aaacggcgtc
     6541 cgcttccagc gggcggcgga gagccagata aatgccgccg gctccgatca gccgggaagg
     6601 attgccggac ttgaaaaatc ctttgttctg actgagatag tcacggcgcg atttttcaag
     6661 tacggccaag ccgtctttct ggatcgccga atcatggatg agtgcggcgt attcaggaag
     6721 atcccgcacg aggatgactg tcgagccgtc aaaataggtc agtttccagg ctgtggaagc
     6781 gataagccgg gcgacaagat ccccggcatc cggccagcat ccgttaagaa caatagcgtg
     6841 cgggttccag ttcgacagaa tggttttcca ggcttccggc tggccgagca gtgcgcgatt
     6901 cagagtctgg ttaaagccgg ttccgtagaa tgatatgcgg gtatcgcaga aaattttgcg
     6961 ccccgggttt tgcagggcga tgtatccgcc gtcatgtgcg atattcatga tattttttgg
     7021 aaaatcgctg cgggacagga ctcgggctgc ggcgaccgga aaagcgcctt cttcgacgcc
     7081 cagaccgaaa tgagaagcgc tgccgatatt gatgtaggcg cggttcgtga ccagcgagac
     7141 gattgaagaa atcatcagaa tcagggcaac ggccgaaagc agcttgtgaa gcatcccttc
     7201 actggctttg aacatggtgg tgagtgtacg gacgagatat tcgctgaccg cattgaagct
     7261 cagcaccata aaggggaacg caaggaaagc aaacagaccc agcgagccga tcgatcgtac
     7321 agtcagaaat gcaccgacca gcgccagcat ggtgagcatg gccggcagcc gcttttgcag
     7381 cgtaatcagt ccgcctgcgc ccagtatcag tgcgaagacg gtcaggttgc tgatataacc
     7441 ctgcgggaag aagcagctga agagagaaat ccactccagt ccgtctgtgc cggtcagcag
     7501 aacccagttg gcgaaaatgt ggcggtgcag attaatcagg ttgggattga ttaaggttgc
     7561 cgccagtgca actgcggaca gtccgaaaag tttggcggtc agcggggtta tcatggcgga
     7621 acgcgagggc tgccgggttc cctgccagtt ttcgaaggcg aaaaacagaa tcagtaccgg
     7681 accgaagagg aaagaagggt gcatgttggt ccacagcacc tgaaagatca gcaggagagc
     7741 ggccagcagg ttgaagttct tgatgcggta aaggcacgtc aggaacagcg ccgtgaaaag
     7801 catgaaaaat acggtcggat ccgggttgaa taccgggaat atcagtcgcg cgcaaagcag
     7861 cagcgccagc ccctggctga gcgggccgcc ccattctttg ctgaagcgga acatcatgat
     7921 gaacgccgcc agtaccgcca gaatgtgaac caccgtaacc agcggggcgc cgccgagcga
     7981 ccagagcaga tagaccaggc tgttatagag cggattcatg ttgatccact gctggccggc
     8041 catggtgtag gagagcgtat cggcgttggc gctgccggcc tgtcccagtg cgatgtgggt
     8101 gaaaatttcg gggctgctga tggtgcgcat gccggtgagg gcgagaagga tgaagccgag
     8161 aagaaccaga acaaggccga gtgatttttt gttactcata aagcaataac cccctgtttg
     8221 aaatgcaggg tcgaatttta gggactcaag cattttttac aagcaggaat cctgagagag
     8281 gctaaaagag attcatttgc gaatcgtcgg tcttcttccg acgggtgcgg tgctgtgcca
     8341 gcttcggagt tccttcggct tcgaactcgc cttcttcaag gttatgcaga atctcagcgg
     8401 cccgttccag tacggaagcc ggcagtcccg ccagccgcgc gacgtggatg ccgtaactct
     8461 tatccgccgc gccgggaaca attttgcgca ggaacgtgat ttggtctccg cgctccttga
     8521 ccagcacatt ggcgttctgc acccccggca tggtcaggct cagatccgtc agctcgtggt
     8581 aatgggtggc aaacagcgtc ttggcgcgca cggccggatt cgtacagaga aactcagcca
     8641 ccgaccacgc gatgctgatt ccgtcgaacg tactggttcc ccggccgatt tcatcaagga
     8701 cgatcagact cttcggtgtc gcattattca gaatgttggc tgtttcctgc atttccacca
     8761 taaacgtgct gcgtccgcgc gccaggtcgt cgctggcgcc gacgcgggtg aaaacgcgat
     8821 caaccagccc gatccgcgct tccgacgccg gaacgaacga gccgatatgc gccataatcg
     8881 tgatcagcgc cacctgccgg atataggttg atttacccgc catgttcggg ccggtaatga
     8941 caatcagctg gtggtcggtg cagttgagcg tcgtgtcatt cggcacaaac cgctcggcat
     9001 ccggcatctg ttcgaccacc gcgtggcggc cgtcgcagat gatgagttcg tcgccggcac
     9061 tcattgcggg ccgcacatag cggcgggtca gcgcattttc ggcaaaagag gccaggacgt
     9121 cgacttcggc agcggcgcgg gcattgcgct ggatctcgtc aatatgcttt acgacctcat
     9181 cgcggatctg cgtgaacagt tcgaactcca gcgctacggc ccgctcctgc gcgccgagga
     9241 ttttattttc atattccttc agctccggcg taatgaaccg ttcggcattc gtcatggtct
     9301 gcttgcgggt gtaggtggcc ggaaccatcg aaagattgct gttggtgatt tcaatgaaat
     9361 agccgaacac ctgattgaag cgcaccttca gcgacttgat gccggtgcgc tcctgctccg
     9421 ccgtctgaaa ttccgccagc cactgcttgc ctttgccggc cgcgtcgcgc agttcgtcca
     9481 gctctgccga aaaaccgcgc cggatcattc cgccgtcttt ggttgaaaca gcaggctcgt
     9541 cgtcgaccgc ctcattaatc atctttccga tacccgggag ttcggtcagt gatttttcca
     9601 gcgcctgaaa atttccgctg gccgccagca gttctttaag cttcggcagc tgatcgagcg
     9661 attcgccgac cgcacgcaga tcgcgcgcgt tgccgccgcc cgaaccaagc cgcgacataa
     9721 tccgttccag gtcgcgcatt tcgccgagca gttcgcggac gtgcgccagc agtgtacgat
     9781 tggctgtgaa cgcttcaacc gcgtcgagcc gcgcattgat ctccgaaaga tcgcggagcg
     9841 gacggacgat ccagtcgcgc agcattcgcg cgcccatcgc cgtgcgcgtc gtatccagca
     9901 ccgaaagcag cgtggatttc ggcccctgcc gcgccggatt gagcggcgcg accagctcca
     9961 gattcatcac cgtcgtctca tccagcagca ggtaatcatc cggattcttc acctgaatgc
    10021 gccggacgtg cgcgaggttc cggcgcagcc cggtattcac ataataaagc accgcacccg
    10081 ccgcgcgcag gccgagcggc gaattctcgc agccgaaccc cttcagtgaa tgcaccttga
    10141 aatggcgcag aagaacgtcc tcagcgctct tgaactcaaa cgtccaatcc tcgccgacag
    10201 ttttaagcgt ccggccgacg accgccagaa tttcagcatc atccgccgcc gcttcggcga
    10261 cgatgcattc ctgcggcgcg tagcgcgcga ggtttccgga gagcgcacct gcgctggccg
    10321 actcttcaat ccagaacgcg ccggtggaca ggtcgagcat cgccaggccg aaccggccgt
    10381 tttcgcggac gagaccggcg agataattgt ttttcgtgcg gtcgagcgcc gtttcgtcca
    10441 gcaccgtgcc gggcgtcacc acgcgggtga tttcgcgctt cacgatacct ttagcggcgg
    10501 caggatcttc gacctgttcg cagatggcga ccttcttgcc ggcttcgatc aattgcgcca
    10561 gataaccctc ggcggcatga tacggaattc cgcacatcgg aatgtcatgg cgccgggtca
    10621 gcgtaatatc gagaatgcgc gccgcctcct tggcgtcctc catgaacatt tcatagaaat
    10681 cgccgaggcg gaagaaaagc agggtgttct ccggaagttc acggcggatg cgccggtact
    10741 gatccatcat cggtgtgctt ttctctgcca tagtccgcac acgctaacaa agcacctttc
    10801 caatgtctgg gattttccgc tcaaaattac gcgtggtaat cctgaatcgg gcggacagtc
    10861 cagtcgttgc cgtcaattgc tttgatggcg ccgagtgtgg cgcgggcgcc ggactcggtg
    10921 gtgacgatcg gaatgttccg cagaatgcac tcggaacgga ttttgatttc atccatgcgc
    10981 gaaacggggc cgctgggcgt gttgatcacc atggcgattt ttccgtcctg catgaagtcg
    11041 atgatgttcg ggtgctgtcc gctggcgagt ttacaggttt cggtgacttt caaaccggcc
    11101 gcgcacaacg ctttggcggt accttcggtg gcggtcagcg tgaagccgag atcaacgaga
    11161 gctttgccga tttcgaccat ccagccttta tcgcggtcag tggcgctgag gaatacgcag
    11221 cctttggccg gcagcatctg gccggcggcg atctgtgatt tccagtaggc catttcaaac
    11281 gtcttatcaa tgcccatgac ttcgccggtc gatttcattt ccgggccgag gatcgggtcg
    11341 acgcccgcaa accggttgaa cgggaagacg gcttctttaa cagcatacca gctcagtttc
    11401 gggcggaagc cgacgaggcc gagttctttc agcgttttgc cgacggcgat ttccgtggcg
    11461 atcttcgcca tcggcacgcc agtcgccttc gaaacatacg gcaccgtgcg cgaggcgcgc
    11521 ggattgactt cgatcacgta gatgtcgctg cctttgactg ccatctgcgc gttcatcagc
    11581 cccttcacat tcagttcgag cgccatgcgg gtgcaggccg cttcgatttc gaggacgatc
    11641 ttcggatcga gcgagtaggg cgggatcgag caggcgctgt cgccggaatg aatcccggcg
    11701 gcctcgacgt gctgcatgac gccgccgaca taaacgtctt tgccgtcgca gacgagatct
    11761 acgtcgattt cgatagcgtt ttcgaggaag cggtcgatca gcaccggatg gcccgggctg
    11821 gcgttgaacg ccgcgtgagt gaacggaaca aattcggttt cgtcgtaggc caccatcatc
    11881 gcgcgtccgc cgagcacaaa cgacgggcgg atcataaccg ggaagccgat gcggtgcgcg
    11941 atcttcagcg cgtcatccat cgtcttggcg gtgccgcttt ccggctgctt gagtccgagc
    12001 ttgatgatca tctggcggaa gagttcgcgg tcttcagcca tggcgatgct cttcggcgag
    12061 gtgccgagaa tcggcacgcc ggcggcgagc aggcggtcgg ccaggttcag cggcgtctgt
    12121 ccgcccatct gaacgatcat gccgatgggc tgttcctgtt cgtagatgtt catcacatct
    12181 tcgaaggtga gcggctcgaa gtagagtttg tcagaggtgt cgtagtcggt cgaaacggtt
    12241 tccgggttgg agttgaccat gatggcttcg tagcccattt cgcgcagcgc tttgacggta
    12301 tgcacgcagg agtagtcgaa ctcaattccc tgtccgatac ggttcgggcc ggagccgaga
    12361 acgataatgc tctttcttcc ggttttgcgg atttcgttga gatcgccgta actggaataa
    12421 aaatacggcg tggcggcttc gaattcgcct gcgcaggtat ccaccagacg gtaaaccgga
    12481 atgacgccgg cttttttgcg ctcttcccgg aacgccagcg gatcctgccc gcgcagtacg
    12541 gcgatctggg cgtcggagaa accgttgagt ttgattttcc ggagcagatc cgcgtcgagc
    12601 ggcttgtctt tcggcgcatc gagaatttcg cgctccattt cgacgatctg cagaatgtta
    12661 tcgataaacc acggatcaat cttggtgatc tcggcgatct tctgaaccga gtaaccctgc
    12721 ttgagggcga ctttgatggc gatacagcgc tcggtcttcg tgcgggtcag ccactcatca
    12781 agccgcgcca gcggtacttt ggcttcggcc ttcaggtcga gaccatccac gccgatttcc
    12841 agcgaacgga acgctttctg gagtgcttct ttaaagttgc ggccgatcgc catcgtttcg
    12901 ccgacagact tcatgctgac gccgagatgc gcatccgccg acgggaattt ttcgaatgcg
    12961 aagcgcggca ttttgacgac gcagtagtcg atggacggtt caaaacaggc gggcgtttct
    13021 ttggtgatat cgttcggcag ttcatcgagc gtgtagccga cggcgagctt ggcggcgagt
    13081 ttggcgatcg ggaagccggt ggctttggac gccagcgccg acgaacgcga tacgcgcgga
    13141 ttcatttcga tcacggccat gcggccggtt tccggatgaa tgcagaactg cacgttcgaa
    13201 ccgccggttt ccacgccgat ggcgcgcatg atcttgatcg atgcattgcg catagcctga
    13261 tattcacggt ccgtcagcgt ctgggtcggc gcaacggtga tgctgtcgcc ggtatgaatc
    13321 cccatcggat ccatgttttc gatcgagcag atgatgacgg cgttattttt cttgtcgcgc
    13381 atgacctcca tctcgaactc tttccagccg tagacggatt cttcgag
//
LOCUS       DAOPZO010000039        13342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_5104_length_13342_cov_10.114548, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000039 DAOPZO010000000
VERSION     DAOPZO010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 13342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13342
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5104_length_13342_cov_10.114548"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1262
                     /gene="aroC"
                     /locus_tag="PKI68_04015"
     CDS             183..1262
                     /gene="aroC"
                     /locus_tag="PKI68_04015"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNX52889.1"
                     /translation="MSSTFGTLFRVTTFGESHGKAVGAIVDGCPANLPLTEADIQPQL
                     DRRRPGQSALTTPRDEADTVTILSGTENGITLGTPIALSVNNRDQRPGDYGDMAFVPR
                     PSHADYTYLMKYGVKASSGGGRSSARETIGRVAAGAVAEKWLKMRFGDVSIVSYVSSV
                     GKIVAPDITGELTRETVDANLVRCPDAETAQKMIAAIEKARDEKDSLGGTVTCICRGF
                     PAGLGEPVFDKLEAMLAQAMLSIPAVKGFEIGSGFAGTLMRGSQHNDAFVRKGERLGT
                     KTNYSGGVQGGISNGEPVVFRIAIKPVATIGQSQETTTLAGEPVVLEAKGRHDPCVLP
                     RAIPIVEAMTALVLTDAALRQEYRI"
     gene            1678..2385
                     /locus_tag="PKI68_04020"
     CDS             1678..2385
                     /locus_tag="PKI68_04020"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX52890.1"
                     /translation="MNELPAAQTHLEAAIDAFPSITGVRHYLGEVYLKRQYFEKAVEQ
                     LKKAQEEEPSIEVLNNLGVAYIGIEEYGQAEALLKQVVRQKPDLAGCYKNLAFLYQKA
                     GRTNDAVLSFEKYFSLVPKDVPLIQNYVAYLTAAGRTPDAIAFLERIESSSDPLAVNL
                     LLARTAAQHADTGRAVHALRDAARFLSPRQTIAEMHDATFDKIAHTEPFEALLYQLEL
                     AEVSLSTNLNTKGTSKN"
     gene            2449..2525
                     /locus_tag="PKI68_04025"
     tRNA            2449..2525
                     /locus_tag="PKI68_04025"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2483..2485,aa:His,seq:gtg)
     gene            2561..3664
                     /locus_tag="PKI68_04030"
     CDS             2561..3664
                     /locus_tag="PKI68_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX52891.1"
                     /translation="MHKKKDEIRDIYQGSSHDFEKDFTPQIAQELIETRQRDLRRSRV
                     ASLFFGSLVIVLSVTLIAVVIRDFLSEHKPSRKNRTEEKPYIPRYSLPADALWVMDYQ
                     MAVAQMDDNEKPGPKPVSTKWIKKAAYHIIMGQQALAINEPAQALEHFQKVVAIYPDI
                     EGLHRAVGMLYLQRKEYPQAVQHLEKALREEEVFDVVSNLGSAYIGTEEYDKAEKYLK
                     RALELQPENPVCYKNMAVLYQKMKRDNDAIFHFEKYLDLQPNDIDTMQTYALYLTRIG
                     RWKEAATFLTELTKEVTNVAPVYFLLAQVQVQNGQQDKAIDALKRGIQLIDPELALAW
                     MSRAEFNSVRNTGEFKTLTDQLEIATVSLEKKH"
     gene            complement(3668..5557)
                     /locus_tag="PKI68_04035"
     CDS             complement(3668..5557)
                     /locus_tag="PKI68_04035"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNX52892.1"
                     /translation="MILSQQVRRPLVGVALSVAAGLTIQRCAGGSPLLLLSITAFVLA
                     AACRAVFYRQTTVLIYIAFGLLAATHSAIEKMPAFSRSALPAAEVNFHEQELTGTIED
                     EPAVSDAAGTVSFLFHAAAVNCAGERRPADAVLRVYLKDPAAPVRFGEQWRLKGRYTG
                     YENPRGGADGVLSASGENACRIKPAQDSFAERCYEVRRCAASLLRPGVEAFSEQTQLL
                     YAMLLGYRQAIPPDLYQLFTRTGTLHIFAISGQHVVILAAIFIAGLKIFGLSRPRWGL
                     LLMPVLFLYIFTTGLQPSALRALTMAAVFFMAPLAGRRPDAPSSIALATILLLALQPA
                     NIGDPGFLLSFVVVCGLIMVHGWAVRQLSGLRLAGWDATLKKLSGSNPAALLLRSTGM
                     LMLTSLAAWLFSAPITALFFNTLSPVALIGNLAVIPLTFMIMLAGCLALLGGVLFLPL
                     AALFNQANLFFISLLIWIVHRLAALPGACRAVRAPSVPVTALWYAGLVLFFAGPVRWR
                     KGALVLVFLSAMFWGAEHFEMSPGIKILREGDSIMALRLPGSRWVLVTDGNSFSTGRT
                     IRRLQKEGINRLHALVVTDALADAGAIRQLQDIFRPAETQTAGRGKKLNWPVNGGVVC
                     LSPVR"
     gene            complement(5568..6842)
                     /locus_tag="PKI68_04040"
     CDS             complement(5568..6842)
                     /locus_tag="PKI68_04040"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX52893.1"
                     /translation="MKFFRKKDTPPAGPRPQISSPDSGFYSQHAGESPLDEYSNGTRG
                     RQTHRTSRHSPARIPETSNRAGSWAIFLLLLRAVLIVVLLVGGFFALKLALDYAAKPS
                     KKELRRREATAALMEKSLPPSSGLVKEQLPEELTISPELIGQRLEQWSQTDRHFRSAE
                     ALSRRGIDDEAMQELGQALRASPNNRAAQQLLLNIYMRKGLYAEAVPLCIRLLDQDGW
                     QQNLQMNLLQALQESGQTEACLLLADRILLDQPNNVTVLTAAADGRIAQGDKDAALAL
                     FEQILVKDAKNAGALEGCGRIYSERGDYQKAVPYYLELVRIDPKPVYYQTLARCYSQQ
                     NQAGKAVIFMGQAASLFGETTVAPWLRDTVFDPVRETVEFRSFADRLVGVETRKAIEA
                     INQREAEKAETPAPDGGKIESPRQLDLKTLKK"
     gene            6950..8041
                     /locus_tag="PKI68_04045"
     CDS             6950..8041
                     /locus_tag="PKI68_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNX52894.1"
                     /translation="MELFSLILLSVLALLVLVQIGLALHGSTRQDTRLEMLTRGLDTL
                     TGQIDSRTRKSEEAVQTQIEKLIASNNQRLDQIGEKVEQRLDKGFRETTTVFSDVLKR
                     LALIDKAQEKIDKLTVNVVSLQEVLSDKRSRGAFGEVQLSALIANMLPENSYSLQHTF
                     PNGVRADCVLFLPEPTGTICIDSKFPLESYRRMTDLTLGDADRTAAERQFRQDIRKHI
                     KDIAAKYIIPGETSDGAMMFIPAEAVFSEIHGHCPELVEEAQHARVWMTSPTTMMAVL
                     TTARAVLKDSATRQQVHLIKEHLVALAMDFTRFQDRMDKLAAHIGQAHKDANDVQTSA
                     RKITSRFGKIEKVELSGDRPALPDDAAEE"
     gene            8162..8332
                     /locus_tag="PKI68_04050"
     CDS             8162..8332
                     /locus_tag="PKI68_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX52895.1"
                     /translation="MAYVISNDCTMCGACESSCPVSAISAGDGKYVIDAGSCVDCGAC
                     AGTCPMGAISAG"
     gene            complement(8382..8834)
                     /locus_tag="PKI68_04055"
     CDS             complement(8382..8834)
                     /locus_tag="PKI68_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HNX52896.1"
                     /translation="MHQFVHISLWAAVTGWLVAQTTKMVICLVQSRRLNFRYLVSTGG
                     MPSAHSAMACAVATTIGLTEGFNSPLFTLGFCFAAVIMFDAQTVRAAAGKQARLLNQI
                     VDELFKEHHLSEVKLKELLGHTRLEVFFGMLTGIVSALALLRFFPPAQ"
     gene            complement(8911..9810)
                     /locus_tag="PKI68_04060"
     CDS             complement(8911..9810)
                     /locus_tag="PKI68_04060"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNX52897.1"
                     /translation="MKKSFSRNDLLHFVPSHETFVGVDSDGCVFDTMETKQKVYFHPL
                     IIKLWGLEAIEPQLRQAAEFVNLYSSRRGQNRFPALLQTFDLLEEWPEVVATGVKLPE
                     RQGLRDYCNSGLPLGNKTLIAEAERTGDPGLAKVVEWSLAVNKAIETNMKKIPPFRGV
                     RESFEKIRLRSDLAVVSQTPEQALIKEWEENGLTQFVRIIAGQELGTKAEHLQMLTGG
                     RYRPDKVLMIGDALGDLRAAASVEACFYPINPGHEEASWKRFAEEAYDRFLAGTFRGA
                     YQQKLNAEFEALLPATPPWSRSA"
     gene            9945..10637
                     /locus_tag="PKI68_04065"
     CDS             9945..10637
                     /locus_tag="PKI68_04065"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX52898.1"
                     /translation="MSKLFTILKHVYRILPTTLRKTGPLRRLAFRIMEKIGDRDDIYS
                     AKYYQTLVEPYARKSAPSMAQSIVETFHPESVIDIGCGSGALLVALRKLGVRRLLGLD
                     YADAGLDIARARGLDTRKFDIVTEKLLYSACYDVVVSTETAEHLPETAADRYVELLCS
                     LAPLVIFTAARPGQGGIGHLNEQPPEYWIELFKAHGFHLAEKTTADWQTAWTAAGVAN
                     FYTRNLMVFQRT"
     assembly_gap    10695..10794
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10795..11094
                     /locus_tag="PKI68_04070"
     CDS             <10795..11094
                     /locus_tag="PKI68_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52899.1"
                     /translation="LMKSTSLTDHLSAFLFWDVDPSGVNPEAHRQFIIPRVMERGTLT
                     DVKTVLEYYGGETVKSVLLNAAGLSAKTVSFFANQFNLPREAFRAFRNPSGCWNP"
     gene            11091..11699
                     /locus_tag="PKI68_04075"
     CDS             11091..11699
                     /locus_tag="PKI68_04075"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNX52900.1"
                     /translation="MKLHTETVSGELLSLLQKLMKEDWLNPFALAGGTALALRFGHRT
                     SIDLDLFTGSTFDAPAVADLLKAAYGMREAATAQNTVRGIIDGIKIDLIAHRYPLLNP
                     LEEAGGIRMFSCEDIAAMKLNAITNRGCKKDFWDYAELLKTYSRDEMLSFFARKYRND
                     SLWNVEKSLTYFDDADNDPDPRDLRGLNWKEIKQTILASARL"
     gene            11778..12191
                     /locus_tag="PKI68_04080"
     CDS             11778..12191
                     /locus_tag="PKI68_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52901.1"
                     /translation="MKLLRPIGISLLSGCILVVTGCASILERQTIRNTNNVGQSMWEY
                     DRGPYPGIGLYVSNFQQSKDSQESRNTDFDEVGPVEAVVLGIIVTPFWIADVILCTAL
                     DTVLLPYDLVMWEKENTNNSTSASTASNQPALRTD"
     gene            12319..>13342
                     /gene="purD"
                     /locus_tag="PKI68_04085"
     CDS             12319..>13342
                     /gene="purD"
                     /locus_tag="PKI68_04085"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNX52902.1"
                     /translation="MKVLVIGNGGREHALCWKLKNDSRKPELFCAPGNAGTAQLGTNL
                     ALSATDVAGIVAWAKANKPDLTVVGPEAPLCAGLADALEAAGFRVFGPRKAAAELEGS
                     KKFAKEIMQAAGVPTAACAVFTDADKACEYVRRKGTPIVIKADGLAAGKGVTVAETVE
                     QAEAAIQDALGGAFGDAGSRVVIEDFLVGEEASILALIDGEHVVLLASSQDHKRVFDG
                     DLGPNTGGMGAYSPAPVATDAVLDIVRTEVYGRTLAELKKRGITYKGVLYAGLMIDNG
                     RPSVVEFNCRFGDPETQAVLARWDGDIIPALEACIDGTLSEDLISWKKEQSVCVVMAA
                     GGYPGDY"
BASE COUNT         3104 a         3892 c         3552 g         2694 t
ORIGIN      
        1 aagcgtgtgt acccttaaaa ggtaccgggg gttcgaatcc ccccctctcc gccatattgc
       61 cgtcagccgg tccgtccgac tcgccctgaa atatctgtaa aaaccggcag aattttttcc
      121 aatgtttgag aaaagggtgt ataaagcctc ccattgtttg gaagattatc cggagaacgg
      181 ttatgagcag tacattcggc acgcttttca gagtgaccac gttcggcgaa tcgcacggta
      241 aagccgtcgg cgcgattgtg gacggctgtc cggccaatct gccgctgacg gaagcggata
      301 ttcagccgca actcgaccgc cgccggcccg gacaaagcgc gctgaccact ccgcgcgatg
      361 aagcggacac ggtgacgatt ctttccggaa ctgaaaacgg tatcacgctc ggaacgccga
      421 tcgcgctttc cgtgaataac cgcgaccagc gtcccggcga ttacggggat atggcctttg
      481 tgccgcgtcc gtcgcatgcg gattacactt atttaatgaa gtacggcgtc aaagcgtcga
      541 gcggcggtgg acggtcgagc gcacgcgaga cgatcggacg cgtggcggcc ggtgctgttg
      601 ctgaaaaatg gctgaagatg cgtttcggcg atgtttcgat tgtctcttat gtgagttcgg
      661 tcggaaaaat cgtcgcgccg gacatcaccg gagaactgac tcgcgagacg gtggatgcca
      721 atctggtgcg ctgtccggat gcggaaaccg cccagaagat gattgcggcc attgaaaaag
      781 cccgcgatga aaaggattcg ctgggcggca ccgttacctg tatctgccgc ggtttcccgg
      841 ccggtctggg tgaaccggtg ttcgacaaac tggaagccat gctggcgcag gccatgcttt
      901 ccattccggc ggtaaaagga tttgaaatcg gctccggatt tgccggtacg ctgatgcgcg
      961 gttcgcagca taatgatgcg tttgtccgga aaggcgaacg gctgggaaca aaaaccaatt
     1021 acagcggcgg tgtgcaggga gggatttcga acggcgagcc ggtcgtattc cggattgcaa
     1081 ttaaaccggt ggcgaccatc ggacagtctc aggaaacgac gactctggcc ggcgagcctg
     1141 ttgtgctgga agccaaaggc cgccacgatc catgtgttct gccccgcgca attccgattg
     1201 ttgaggctat gactgcgctg gtattgaccg atgcagcact tcggcaggaa taccgcattt
     1261 gaatcttagt gtatgaacag ccaagataca gatattcggg acatttaccg gtcagaagac
     1321 tctgcgcctg aatcagcgcc ttcgcgggaa atgcaggatc ttattgaaag cgaaatacgc
     1381 agacggcgga ttttcaatac cgttgccact ttgctgactc tcacactgac cgtttttttg
     1441 atgctttctt ttgttaaaga gcagttacct gccgcttctg ataaaacagc gcctgttgca
     1501 gaaaaacatc cttattttgc cgcttataca ttgccggcgg aggaacagtg ggcgctggaa
     1561 taccgtcagg ttgcacagca agccggcggc agcgagccgc ccggccccaa acctttttcg
     1621 accaagtggg taaaaaatac agcatatcac cttattatgg gagaacaggc gcttcgaatg
     1681 aacgagctgc ccgccgccca gacccatctt gaagcggcca ttgacgcctt cccctcaata
     1741 accggggtcc gccactatct gggtgaggtt tatttgaaac ggcagtattt tgaaaaagcg
     1801 gttgaacagc tgaaaaaagc gcaggaagaa gagccttcca ttgaggtgct caataatctc
     1861 ggcgttgcgt acatcggaat tgaagagtac ggacaggccg aagcgctctt gaaacaggtt
     1921 gtccggcaaa agccggatct tgccggttgc tataaaaacc tcgctttctt ataccagaaa
     1981 gcgggccgga ctaatgatgc ggttctctct tttgaaaaat atttttctct tgtcccgaag
     2041 gatgttccgc tgattcaaaa ctatgtcgcc tacctgactg ccgccggacg gacacccgat
     2101 gccattgctt ttcttgaacg gattgaaagc agctccgacc ctctggctgt caatctgctg
     2161 ctggcccgta ctgcagcgca gcatgccgat acagggcggg ccgttcatgc cctgcgggat
     2221 gcggctcgtt ttctctcccc ccggcagaca atcgcagaaa tgcacgatgc tacctttgat
     2281 aaaattgccc acaccgaacc ttttgaggca cttttatatc agcttgaatt ggcagaagtg
     2341 tcgctttcca caaatttgaa tacaaaaggg acatcaaaaa attaaaacgg gtagatatat
     2401 tttgctttct attgattttt tattactcta ccacgctttc gaatgatggt gggtgtagct
     2461 cagctggtta gagcgtcaga ttgtgattct gaatgtcgcg ggttcgaatc ccgtcactca
     2521 ccccatattc tgcctgtatg gagagcacga ggagccgctc atgcataaga aaaaagacga
     2581 gatcagggac atctatcaag gcagttccca cgacttcgaa aaggatttca ctccgcaaat
     2641 cgcgcaggag ctgattgaga cccgccagcg cgatctgcgg cgcagccggg tggcttcgct
     2701 ttttttcggt tcgctggtca tcgtgctttc cgtgacgctg attgcggtgg ttatccgcga
     2761 ttttctaagc gagcacaagc cgtccaggaa aaaccgcacc gaagaaaaac cttatattcc
     2821 gcgctactcc ctgccggcag acgctctttg ggtgatggat tatcagatgg ccgttgcgca
     2881 aatggacgat aatgagaaac cgggtccgaa accggtttcg accaaatgga taaaaaaagc
     2941 ggcctatcac atcattatgg gccaacaggc gctggctatc aacgaaccgg cgcaggcgct
     3001 ggagcatttt cagaaggtcg ttgcgattta tcccgacatc gaaggcctgc accgcgctgt
     3061 cggaatgctc tatcttcagc gtaaagaata cccgcaggcc gttcagcatc tggaaaaagc
     3121 gctgcgggaa gaagaggtgt ttgatgtggt gagcaacctc ggctccgctt atatcggcac
     3181 agaagaatac gacaaggccg aaaaatatct gaaacgcgcc ctcgaactgc agcctgaaaa
     3241 tcccgtctgt tataaaaaca tggcggtcct ctaccagaaa atgaagcgcg ataatgatgc
     3301 gattttccat tttgaaaaat accttgatct gcagccgaac gacatcgaca ccatgcagac
     3361 ttatgcgctt tatctgacca gaatcgggcg ctggaaagaa gccgccacgt tcctgaccga
     3421 gctgacaaaa gaggtgacaa acgttgcccc ggtttatttc ctgctggcgc aggtgcaggt
     3481 gcagaacggg cagcaggaca aagcgattga cgctctgaaa cgcggtattc agttaattga
     3541 tccggaactt gcgctggcct ggatgagccg cgctgaattc aattccgttc gcaacaccgg
     3601 cgaatttaaa acactgaccg atcagctcga aatcgcaacc gtttcgctgg aaaaaaagca
     3661 ttgatgctca tcggacgggt gaaaggcaga caacccctcc gtttacaggc cagttcagtt
     3721 ttttacctcg tccggcggtc tgagtctccg ccggacggaa aatatcctga agctgccgga
     3781 ttgcgccggc gtccgccagc gcatcagtca caaccagtgc atgcagccgg ttgattccct
     3841 ctttctgtaa ccggcggatt gtacgccctg tgctgaatga attgccgtcc gtaaccagca
     3901 cccaccggct cccgggcagc cgcaaggcca ttatcgagtc gccctcacgc agaatcttta
     3961 tgcccggact catttcaaaa tgttctgcac cccagaacat cgctgacaaa aacaccagca
     4021 ccagcgcccc tttccgccag cgaaccggcc cggcaaaaaa taaaaccagc ccggcatacc
     4081 aaagcgccgt caccggaaca gaaggtgcgc gaacagcccg gcacgccccg ggcagcgcgg
     4141 cgagacggtg aacaatccat atcagcaggc tgatgaaaaa cagattcgcc tggttgaaaa
     4201 gcgccgctaa aggcaaaaac agaacgccgc ccagcagcgc cagacatccg gcgagcatga
     4261 tcatgaacgt cagcgggata accgccagat tgccgatcag cgctaccgga gaaagggtat
     4321 taaaaaacag ggcggtgatc ggggccgaaa aaagccaggc cgcgagcgaa gtaagcatca
     4381 gcattccggt gctgcgcaac agcagcgcgg ccgggttcga tccgctgagt tttttcagag
     4441 tcgcatccca gcccgcgagg cgaagtccgc tgagctgccg gaccgcccag ccgtgaacca
     4501 taataagacc gcatacgaca acaaacgaaa gcaggaaccc cggatcgccg atgttcgcag
     4561 gctgtaatgc cagcaataaa atcgtggcca gtgcaatgga tgacggcgcg tcgggccggc
     4621 ggccggcgag cggagccata aagaaaacgg ccgccatcgt cagcgcccgc aaagcactcg
     4681 gctgcaggcc tgtggtgaaa atatacagaa aaagcacagg catcagcagg agcccccagc
     4741 gcgggcggga aaggccgaaa atcttcagcc ccgcaatgaa aatggcggct aaaatgacaa
     4801 cgtgctggcc ggaaatggca aaaatatgca gcgttccggt gcgggtgaac agctgataga
     4861 gatcgggagg aatggcctgc cggtagccca gaagcatcgc atacagcagc tgcgtctgtt
     4921 cagaaaacgc ttcgacgcct ggacgaagca gcgaagccgc gcagcgacgg acctcataac
     4981 agcgttcagc aaatgagtct tgcgccggtt tgatcctgca ggcgttttct ccggaggcgc
     5041 tcaaaactcc gtctgcaccg ccgcgcggat tttcatagcc ggtataacgg cctttcaacc
     5101 gccactgttc gccgaaccga accggagcag caggatcttt cagataaacc cgtaaaacgg
     5161 cgtcggcagg gcgccgttct cctgcacagt ttacagccgc cgcgtgaaac aaaaacgaaa
     5221 cggtgccggc cgcatcggaa acagcaggct catcttcaat ggtgccggtc agctcctgtt
     5281 catgaaaatt cacctcggcc gccggcagcg ccgaccggga aaaagccggc attttttcaa
     5341 tggcactatg cgtggctgca agcagcccga aggcgatata aatcagtacg gtggtctgcc
     5401 ggtaaaagac ggcccggcag gcagcggcca gaacaaaagc cgttatactg agcagaagta
     5461 agggtgaacc gccggcacag cgctgaatag ttaagccggc agctaccgac agggcgactc
     5521 caaccagcgg gcgccgcacc tgttgagaca aaatcattcc ggtcgcttta ttttttcagt
     5581 gttttcaggt caagctggcg gggagattcg attttgccgc cgtcaggtgc cggtgtttca
     5641 gccttttcgg cttcgcgctg attgatcgcc tcaattgcct tgcgggtttc aacaccgaca
     5701 agacggtcgg caaacgagcg gaattctacg gtttcgcgga ccggatcaaa tactgtgtct
     5761 ctcagccatg gagcaacggt cgtttcgccg aacaggctgg ccgcctgtcc catgaaaatg
     5821 accgccttac ccgcctgatt ctgctgcgaa taacaacggg caagcgtctg atagtagact
     5881 ggttttggat caattcgaac cagctcgaga taatacggaa ccgctttctg gtaatcgccc
     5941 cgctctgaat aaatgcggcc gcacccctct aatgctcctg cgtttttagc atcttttacc
     6001 agaatctgct caaaaagcgc caacgcggca tctttgtcgc cctgtgcaat ccgcccgtcg
     6061 gcggcggcag tgagcacggt tacattattc ggctgatcca gaagaatccg gtctgccagt
     6121 aacagacagg cctcagtctg cccgctttcc tgcaaagcct gcaagagatt catttgcagg
     6181 ttctgctgcc agccgtcctg atccagcaga cggatacaca acggaacggc ttctgcatag
     6241 agccccttcc gcatatagat attcagcaga agctgttgcg cagcacggtt attcggtgac
     6301 gcgcgcaaag cctgtcccag ttcctgcatc gcttcatcgt cgataccgcg gcggctcaga
     6361 gcctcggcag agcggaaatg ccgatccgtc tggctccact gttccagccg ctgtccgatc
     6421 aattccggac tgattgttaa ttcctcagga agctgctcct tcacgaggcc ggatgacggc
     6481 ggcaatgact tttccatcaa cgctgcagtg gcttcccgcc ggcgcagttc tttcttagaa
     6541 ggttttgcgg cataatccag cgccagcttc agagcaaaaa atccgccgac aagcaacacc
     6601 acgatcagaa cagcccgcag cagcaataga aaaatagccc agctgccggc acggttgctg
     6661 gtttcgggaa tacgggccgg gctgtgtcgt gaagtgcggt gcgtctgccg ccctctggta
     6721 ccgttggaat actcatcaag cggagattct ccggcgtgct gtgaatagaa tcctgaatcc
     6781 ggcgatgaaa tttgaggacg ggggccggcc ggcggagtat cttttttgcg gaaaaatttc
     6841 atggttttat gcgctccggt tcaaaaataa aacggttgca gtatcgggcg ggccgcgtaa
     6901 agtgtcaagc actgcgggct gctgcacgga gcccgtccgg agatgtttta tggaactttt
     6961 cagtctaatc cttttatcgg ttctcgccct gctggtactg gttcagatcg gtctggcgct
     7021 gcacggaagc acccggcagg atacgcggct tgaaatgctc acacgcggtc tggatacgct
     7081 gactggacag atcgattccc gcacccgtaa aagcgaagaa gcggttcaga ctcagattga
     7141 aaaactgatc gcctccaata atcagcggct cgaccagatc ggtgaaaaag tcgagcagcg
     7201 gctcgacaag ggattcaggg aaacaacgac cgttttcagc gatgttctca aacggctggc
     7261 gctgattgat aaggctcagg agaaaataga taaactgacc gtaaatgtcg tcagcctgca
     7321 ggaagtgctg tccgacaaac gctcgcgcgg cgctttcggt gaagtacagc tcagcgcgct
     7381 gattgcgaat atgcttcccg aaaacagcta ctctcttcag cacacattcc cgaacggcgt
     7441 gcgcgccgac tgcgtactgt ttctgcccga accgaccggc accatctgta ttgattcgaa
     7501 attcccgctg gaaagctacc ggcgcatgac cgacctgaca ttgggcgacg ccgaccgcac
     7561 cgccgccgag cgccagttcc ggcaggacat cagaaaacac atcaaagaca tcgccgccaa
     7621 atacatcatt cccggtgaaa cctccgacgg cgccatgatg tttatccctg ccgaagccgt
     7681 cttttctgaa attcatggtc actgcccgga actggttgaa gaagcacagc acgcccgggt
     7741 ctggatgaca tcccccacca ccatgatggc cgtactcacc accgcgcggg ccgtactgaa
     7801 agactccgcc acccgccagc aggttcacct catcaaagaa catctggtgg cgctggcgat
     7861 ggatttcacc cggttccagg accggatgga taaactggcg gcgcacatcg gccaggcgca
     7921 caaagacgcg aatgacgtgc agacctccgc gcgtaaaatc accagccgct tcggtaaaat
     7981 cgaaaaagtg gagctttccg gcgaccgccc cgctctgccg gacgacgcag ccgaagaata
     8041 agcctccggt cttaggccaa agtccgctcc gtttccggtt tgaacccgcg gaatgatgtg
     8101 gtaccttgcc cgccgtcaga agcagggaca ggtggcctgc tgttaaccta aggagagtat
     8161 catggcttat gtaatttcta acgactgcac gatgtgcggc gcttgcgaaa gctcctgccc
     8221 ggtgtcagcg atcagcgcgg gcgacggcaa atatgttatc gacgccggca gctgcgtgga
     8281 ttgcggcgcg tgtgcaggaa cctgcccgat gggcgcgatc agcgccggct gaacccggtt
     8341 gcagtgtaca cgaaagcctt ccgctccggc ggaaggcttt tttattgcgc cggaggaaag
     8401 aagcgcagca gcgccagcgc tgaaacaatg ccggtcagca taccgaagaa tacttccagc
     8461 cgcgtgtggc ccagcagctc cttcagctta acttccgaca ggtggtgttc cttgaacagt
     8521 tcgtccacga tctgattcag cagccgcgcc tgcttgccgg ccgccgcacg caccgtctgc
     8581 gcatcaaaca tgatcacggc ggcgaaacag aaacccagcg taaacagcgg cgaattaaac
     8641 ccttccgtca aaccgatggt cgtcgccacc gcacaggcca tcgctgaatg cgcgctcggc
     8701 attccgccgg tactcaccag ataacggaaa ttcagacggc ggctctgcac cagacaaata
     8761 accatcttgg tcgtctgcgc aaccagccag ccggtgacgg cggcccaaag agaaatgtga
     8821 acaaactgat gcatcaactt cacctgcctg ggttatccgt ggaaacaagg cggataataa
     8881 agagcatatc gccggacggc gcaacctttt tcaggcagac cggctccagg gcggagtcgc
     8941 cggaagcagc gcctcgaact ccgcattcag cttctgctga tacgccccgc ggaaggttcc
     9001 ggcgagaaac cggtcgtacg cttcctccgc aaaccgcttc cagcttgctt cttcatgccc
     9061 gggattgatc ggataaaaac aggcctcgac cgacgcggcg gcccgcagat cgcccagcgc
     9121 atcaccgatc atcagcacct tgtccggccg gtaccggccg ccggtcagca tttgcagatg
     9181 ctccgctttc gtgccgagct cctgcccggc aataatccgg acaaactgcg tcaggccgtt
     9241 ttcttcccac tccttgatca gcgcctgctc cggcgtctga gaaacgaccg ccagatctga
     9301 acgaagtctg attttttcaa aactctcacg gacaccgcgg aacggcggaa tctttttcat
     9361 attcgtttcg atcgctttat tcaccgccag cgaccactca accactttcg ccagtcccgg
     9421 atcgccggta cgctccgctt ccgcaatcag cgtcttattg cccagcggca gaccggaatt
     9481 acaataatcc cgcaggccct gccgctccgg cagcttgact ccggtggcga cgacttccgg
     9541 ccattcttcc agcagatcaa acgtctgaag cagcgccggg aaccggttct gtccgcgccg
     9601 gctggaataa agattcacaa attccgccgc ctgccggagc tgcggctcga tcgcctccag
     9661 cccccacagt ttaataatca gcggatgaaa atataccttc tgcttcgttt ccatcgtatc
     9721 gaaaacgcat ccatcggagt cgacgccgac aaatgtttcg tgcgacggaa caaaatgaag
     9781 cagatcgttg cgggaaaaac tttttttcat gcgcgagacg ctacccgttt gcccgcgccc
     9841 tgtccagcga gtccgccgta gccgccgaag ctccttcagc gaaggatggg gcgaaggcgg
     9901 aagcttgccg aaacagcctt tccctgctat accgcaggca ttctatgtcg aaactcttca
     9961 ccatcctgaa gcacgtttac cgcatcctgc cgacaacgct tcgcaaaacc ggcccccttc
    10021 gccgcctcgc tttccgcatc atggaaaaaa tcggcgaccg cgacgacatc tactcggcca
    10081 aatactacca aacgctcgtc gagccctacg cccgtaaaag cgccccgtcc atggcgcaat
    10141 ccatcgtcga aacctttcac ccggaatccg ttatcgacat cggctgcggc tccggcgcac
    10201 tgctcgtcgc attacggaaa ctcggcgtgc gccgcctgct gggactggac tatgccgacg
    10261 ccggactcga cattgcccgc gcccgcggac tcgatacccg caaattcgac atcgtcaccg
    10321 aaaaacttct ctactccgcc tgctacgacg tcgtcgtcag cacggaaacc gccgaacacc
    10381 tgccggaaac cgccgccgac cgctacgtcg aactgctctg ctcgctcgcg ccgctcgtca
    10441 tcttcaccgc cgcccgtccc ggacagggcg gaatcggcca cctcaacgaa cagccgccgg
    10501 aatactggat agaattgttc aaagcgcacg gcttccacct cgcggaaaaa accaccgccg
    10561 actggcaaac cgcctggacc gccgccggcg tcgccaactt ctacacccgc aacctcatgg
    10621 tttttcagcg gacttgagcc accgtagacg cggtgtcctc accgcgtttt ctttgcgtgc
    10681 ggaacgcggt gaggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctcatg
    10801 aaaagcacct ctcttaccga tcatctgtcc gcgtttcttt tctgggatgt tgatccgtcc
    10861 ggcgtgaatc cggaagccca tcggcaattc atcattccgc gggttatgga aagaggaacg
    10921 ctgaccgatg taaaaacggt tttggaatat tacggcggtg aaacggtcaa atcggttctg
    10981 ctgaacgccg ccggcctgag cgcaaaaacg gtcagctttt tcgccaacca gttcaacctt
    11041 ccccgggaag ctttccgggc tttccgcaat ccgtccggct gctggaaccc atgaagctgc
    11101 atactgaaac cgtttccgga gaactgctca gcctccttca aaaactgatg aaggaagatt
    11161 ggctgaaccc gttcgctctg gcgggaggga ctgcgctggc tctccgcttt ggccaccgaa
    11221 catcgatcga tctggacctc ttcaccggct caacctttga tgctccggcc gtcgcggact
    11281 tactgaaggc cgcttacggt atgcgtgaag cagcaacagc tcaaaacacg gtacggggca
    11341 tcattgacgg aatcaaaatc gacctcatcg cgcaccgcta tcctctgctc aacccgctgg
    11401 aagaggccgg cggcatacgg atgttctcct gcgaagacat cgccgccatg aaactcaacg
    11461 ccatcaccaa ccggggctgt aaaaaggact tctgggacta cgccgaactg ctgaaaacct
    11521 attcccgcga cgagatgctt tccttcttcg cccgcaaata ccggaacgac agcctctgga
    11581 acgtggaaaa gtcgctgacc tactttgacg atgccgacaa cgaccccgat ccgcgcgacc
    11641 tgcgcggcct gaactggaag gaaatcaaac aaaccatact cgccagcgca cggctctgaa
    11701 ccctattgcc gccggcgtcg ccaacttcta cacccgcaac cttatggttt tcggaaaata
    11761 aattgttgga tttcagaatg aaattactac gcccaattgg aatttcgctt ctttctggat
    11821 gtattctggt agtcacagga tgcgcatcca tcttggagcg ccagactatt cgcaatacaa
    11881 acaacgtcgg tcaaagcatg tgggaatatg atcggggccc ttatcctggt ataggactct
    11941 atgtctccaa ttttcaacaa agcaaagact cgcaagagtc acgaaatacc gattttgacg
    12001 aagtaggtcc tgtcgaagca gtagttcttg gcatcattgt caccccattc tggatagccg
    12061 atgtcattct ctgcacagcc ctagacaccg ttcttctgcc atacgacctt gtaatgtggg
    12121 aaaaagaaaa tacaaataat tcaacaagtg catcgaccgc ttcaaatcaa cccgcgctac
    12181 gcacggattg atttgacggt catgctaacg ttccgccttc gttttccttc gttcccttct
    12241 gtaaaacatt ttgtcccaag cttgtccaaa ccggcgaatt caggctaact atgcgcctcg
    12301 caacgaaagg gttttctgat gaaggttttg gtaattggaa acggcggacg ggaacatgcg
    12361 ctctgctgga aactgaagaa tgattcgcgc aagccggagc ttttctgcgc gccgggtaat
    12421 gccggtaccg cgcagctcgg cacgaacctc gccctttccg ccaccgacgt cgccggaatc
    12481 gtcgcttggg cgaaagccaa caaacccgat ctgacggttg tcggccccga agcgccgctc
    12541 tgtgccggtc tggccgacgc gctggaagcc gccggcttcc gtgttttcgg tccgcgcaaa
    12601 gccgccgccg aactcgaagg cagcaaaaag ttcgccaagg aaattatgca ggccgccgga
    12661 gttccgaccg ccgcctgcgc ggttttcact gacgccgata aagcctgcga atacgtccgc
    12721 cggaaaggca caccgattgt catcaaggcc gacggcctcg ccgccggtaa aggcgtaacg
    12781 gttgccgaaa ccgttgaaca ggccgaagcc gctattcaag atgcgctcgg cggagcgttc
    12841 ggcgacgccg gaagccgcgt ggtgatcgaa gatttcctcg tcggcgaaga agcctccatt
    12901 ctcgcgctga tcgacggcga acacgtcgtc ctgctcgctt cgtcgcagga tcacaagcgc
    12961 gtgttcgacg gcgacctcgg cccgaacacc ggcggcatgg gcgcctactc gcccgcgccg
    13021 gtcgccaccg acgcggtgct cgacatcgtc cgcaccgaag tctacggccg cacgctggcc
    13081 gagctgaaaa agcgcggcat cacctataaa ggggtgcttt acgccgggct gatgatcgat
    13141 aacggccggc cgagcgtggt cgaattcaac tgccgcttcg gcgatcccga aacgcaggcg
    13201 gtgctggccc gctgggacgg cgacatcatt cccgcgctcg aagcctgtat cgacggcacg
    13261 ctgtccgaag atttgatttc gtggaaaaaa gagcagtccg tctgcgtcgt catggcggcg
    13321 ggcggctatc ccggcgacta tc
//
LOCUS       DAOPZO010000040        13261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_5149_length_13261_cov_8.340224, whole genome shotgun sequence.
ACCESSION   DAOPZO010000040 DAOPZO010000000
VERSION     DAOPZO010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 13261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13261
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5149_length_13261_cov_8.340224"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..1072)
                     /gene="obgE"
                     /locus_tag="PKI68_04090"
     CDS             complement(71..1072)
                     /gene="obgE"
                     /locus_tag="PKI68_04090"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNX52903.1"
                     /translation="MKTIVFRDRVKIFVQAGNGGNGIASFRREKFAQFGGPNGGDGGH
                     GGSVILRGDFNLDSLNALYYHPHQNAENGGHGKGNDCTGRCGEDRILTVPCGTVVLDP
                     DTEEFVGEILEDGQELVIARGGRGGLGNPHFKSSVNQAPTKCTPGTPGEERAYRLELK
                     LVADVGLVGYPNAGKSTLLTELTDAHPKIASYPFTTLNPILGTLIYENFNQIRIADIP
                     GLIDGASEGIGLGHYFLRHIERSRFLLFVIDMAAEDGRDPVEDYRSLRRELKAYNADL
                     ANRPSLVLANKMDMPAAAENIKIFRRKTRLKPVEISAGLGEGLDQIKEILYEQFFGK"
     gene            complement(1144..1398)
                     /gene="rpmA"
                     /locus_tag="PKI68_04095"
     CDS             complement(1144..1398)
                     /gene="rpmA"
                     /locus_tag="PKI68_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002778821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNX52904.1"
                     /translation="MAHKKGQGSSTNGRDSQPKRRGVKRYAGQEVTAGSIIVRQVGNK
                     FVAGANVGQGRDFTLFALKHGIVEFDKAGRRVNIREMQIA"
     gene            complement(1417..1737)
                     /gene="rplU"
                     /locus_tag="PKI68_04100"
     CDS             complement(1417..1737)
                     /gene="rplU"
                     /locus_tag="PKI68_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNX52905.1"
                     /translation="MEAYAVIETGGKQYRVEKDTVLSVELLDAAEAGKAIEFDQVLAV
                     SNGSELTIGAPVVAGAKVTATVVENYRGDKVVSFKKKRRQGYRRKIGHRQGLTKIKIE
                     SITA"
     gene            2076..3512
                     /locus_tag="PKI68_04105"
     CDS             2076..3512
                     /locus_tag="PKI68_04105"
                     /inference="COORDINATES: protein motif:HMM:NF006088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNX52906.1"
                     /translation="MTKPLATTLCPLCVDLDGTLVKTDTFAQEMLLLIRTRPLTFFYL
                     LRRISAGLAPFKQRIAGEIQLDPAALPYQFEFLELLKEEYRTGRELILVTASDKAPAQ
                     AVAEYLGLFSDVMASNGVCNLKATRKRDALVARFGEKGFDYAGNAVDDLPVWAVARNI
                     IAVNPSTTVLRALANRSARVFEDRPPLWRVWLRALRVHQWTKNMLIFLPMLLAHELTN
                     PVLYLQAVIAFFSFSLAASTIYIFNDIFDLHADQHHPHKKKRPFASGNLGLGGAVVVM
                     PLLLISFLLAALLSPTFIGILLLYLALTTLYSWWLKQTAVLDVLTLAGLYALRIFAGT
                     AAYGVVASEWLIAFSIFIFLSLALVKRYAELRAAAQQMPDSLQSRGRGYRVVDMPLLG
                     GFGVATGCLSVLVLALYISSPRVIDFYPRPAVLWLLCPLQFYWIARIWLLAGRGEMSD
                     DPLDFAARDLRTWLVVFLSSMTLVLGSF"
     assembly_gap    3622..3721
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3722..5053
                     /locus_tag="PKI68_04110"
     CDS             <3722..5053
                     /locus_tag="PKI68_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52907.1"
                     /translation="WFESNMNTIKKILNSFRIDQCSWAPEQEPKKFHVFALLLIFLLF
                     SGLYGLLGWLFMRLGCFSHVDIFFGADARWMVGDIAEFAANHYRTNTHPLGVLLVNPV
                     GTLLNLLVRSPQTTALLITALTGGGFVVLLVVFLLRLCISKTLAVLCAFLGGLSACNL
                     LWSAVPDYFLLDGISLLFFCWVVTAHPHKLWLVVVSAAIAAGTVTTNLPVVVMIYTVA
                     RHGIRQKLSVMLRDGIKFFVLILALVTMLAVVQDLIYPSSTLFFRTHALQSRTMYMSS
                     ESTIGRFALFVPYLFLFNILFPSVARVSLGAEGLAGIRFPITKLSACTELFTPMGWLF
                     VLLWLVLLGFSLWVCIREKQMAKPLTVLLALCLLFNVALHLFYGRPDEFFLYTPHWTF
                     MVIALLGISLDVISRKFRQVFLAIVIYLLLLVVLLIANNATVIRKILDILE"
     gene            complement(5057..5821)
                     /locus_tag="PKI68_04115"
     CDS             complement(5057..5821)
                     /locus_tag="PKI68_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52908.1"
                     /translation="MTENLQQNTEQKGPFSTLQTGCAFVMLTNMLIYLGVVAFTFNLL
                     DPSKMDASYLAKRGFTARPEPEREEDVAFRQLSKEKREEQKALQTGIIATPGQKQEAS
                     PAGKLSQIEQRKHSPSSPDEPEKPKAALTQSPRRRVGTTTRSAQSRLYYSPVIYHQAN
                     LGQGYSPTRLFVTQILMPQKFETLSVELAPSIDFPTFRLPIADPAGAYLYTPPDPIPE
                     ASRGGLKLTAPPVGAESLYTNGTRKAKEPPPAKDEQ"
     gene            complement(5894..6283)
                     /locus_tag="PKI68_04120"
     CDS             complement(5894..6283)
                     /locus_tag="PKI68_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52909.1"
                     /translation="MMIPDLQSSLICDDVRQERNGKFMLIGLFDAIHAERLPVAFARM
                     CLVTRWCSGEGTFTQRSRIIHPDQKTVLAEGQDVQVQLPSPEAAATSVEVFINITFPE
                     AGTYWVEILLGGDLKIRYPLRVNRIER"
     gene            complement(6280..7041)
                     /gene="budA"
                     /locus_tag="PKI68_04125"
     CDS             complement(6280..7041)
                     /gene="budA"
                     /locus_tag="PKI68_04125"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HNX52910.1"
                     /translation="MRKQFLLLLLPLLAGCATAPKNTLTQISTIDALLAGAYDGQMTT
                     GNLLNYGSLGIGTFDGLEGEMIVIDRQVYQARADGSVCLMPTNTTTPFASVVNFKADS
                     ICHYDGALDEKMFQDFADGLAPNSNLFVAVRFDGHFPLMKVRSVPKQEKPYPPLAEVV
                     QHQTVAEYASVNGTVLGFRCPSFVKGVNVPGWHLHFISDDRTKGGHILSFTTTGGELK
                     LDSCNRFYMALPDQEQFGTLDLSKDRSEELQKVEK"
     gene            complement(7038..8018)
                     /locus_tag="PKI68_04130"
     CDS             complement(7038..8018)
                     /locus_tag="PKI68_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNX52911.1"
                     /translation="MKQLQTLTNSEWLARLGDARGNLSNTLYAMYSGLTGGITLDPAL
                     MTIPADDHLPQRGDGVFESLKCLNGRLYNADAHLARLELSCKSIGMEAPCTRAELTQI
                     ICDTIRAGGKRDCLIRVLVSRGTENMGIDPKLCKGPVLYVIVYRYTARIENGRLKPAR
                     VALSKVPVKPPEFATIKTCNYLPNVLMKLEANQRGLDFVISVDANGNLGEGATENVGI
                     LTPDNELLMPKPDYVLPGTTARRTLDLAKALTADGTLKAAEYRDISPAQAAQAKEIFI
                     LGTTTDVTPVIEWEGRPVGSGKPGPVAKKLLNLLQNDMTPESETLTEVFK"
     gene            8100..9326
                     /locus_tag="PKI68_04135"
     CDS             8100..9326
                     /locus_tag="PKI68_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX52912.1"
                     /translation="MVMETAFAPAERASQEEIRRQYLKLAQLPFVRDFLDAIPNMSVV
                     LNEHRQIVFANRAFAEFLGIKSSDELLGKNHCEAFSCLYADVLGARPGEAVGCVRSRL
                     TEGGCGTTMFCRTCGAVIAIMNSQKLRKLDIQECRMICGEEGLNETALDLRVWSRPIE
                     VSGEVFTVFSVVDISNEKRRKVLERIFFHDVLNTAGGVKGLADLLIQTELSEVEIKDI
                     SSMISESADHLIEEISDQRILSAAESGDLEVSVQEVHSLEVLCRIIRQFHSYSIAKGK
                     VLEVDAATEHFDFVTDPVLLRRVLINLVKNALEAIDEGGTVTLGSYTDRDSVYFTVHD
                     AAAIPPEVQLQVFSRSFTTKGSGRGLGTYSIKLISEKYLRGRVSFVSNAEEGTRFTIR
                     YPKVIQSSGELDETAG"
     gene            9310..9687
                     /locus_tag="PKI68_04140"
     CDS             9310..9687
                     /locus_tag="PKI68_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52913.1"
                     /translation="MKPPVEIICTRCGAEAFLKREPVYEGFTKTGEELSCSACGFIFA
                     AESEVPFKARSAAPKIFTDADRSASVKVFSEDENKCICRYCANYVVNPFVQFCARYKK
                     EVQATDSCPQFSPVQKKPGKPLF"
     gene            complement(9684..10550)
                     /locus_tag="PKI68_04145"
     CDS             complement(9684..10550)
                     /locus_tag="PKI68_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNX52914.1"
                     /translation="MNDLQNHLKRQSEAVNAALDALLPPETERPLKLHNAMRYSIFAG
                     GKRLRPALCIAACEACGGTAEQAMPAACALELLHTYTLIHDDLPAMDDDTLRRGRPTC
                     HIAFDEATAILAGDALLTLAFEVLAGQPQGGALALELARAAGSRGVIGGQAEDIDAEG
                     KAPDAALVEYIHRNKTAALIRAACVMGGMCAGADLQTLKHLAIYGENTGLAFQLADDL
                     LDESATVEELGKDIGSDKERGKMTWPAVHGTEKTAADAKQLTADAVKALQGLKNPARL
                     GQLAEFIAGRTY"
     gene            10691..12412
                     /locus_tag="PKI68_04150"
     CDS             10691..12412
                     /locus_tag="PKI68_04150"
                     /inference="COORDINATES: protein motif:HMM:NF021347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HNX52915.1"
                     /translation="MKDRKEFYNLLAEIDGKPFAEYERLLGDFDFARYVIKCTKIGTA
                     NGAENPVFNIRIPQSIAELPGHLYDSPVRRTALEDFLTRNVAESAGRIARFNESGWAR
                     RNIIVAEPGQKILPRTSVVVTKEYIDVRLRIALPYKVYAAGEHLIDGEMARKVFFDDL
                     PEVVSSSIFFCNIDLDEAESFVNGMEDADRVRQTLSTLGFVSFVGEGSLLAREQDSDL
                     PDYDHIAPFEVSEAAQTEIDVPNAGKIIGLGIPAGLTIVLGNAANGRKDFMSAIAAGV
                     FNHIPGDGRETVVTVSDAVQIAADRGRSVQEVNITPFLSESETGNPASYSTRSADSFI
                     SQAAATIEALEVGARVLIVDENTSAPAFLTTDARIAGLLGKTPRVSLAQRARQMVDEL
                     GISLVIGGENLVAEYIPFANTVLKVEDFQVTNITEEAKALNLPVPPEAPVVNLGSMLG
                     RSRWIMPSSIDAAVGSIDQVIEAIDLKAIQFGRSVIELDSVPQIADVNQTLTIGLLLY
                     YAKLRYMQEGYPLREMLDMIDRDLSSEGLGTIARDLRGDLARPRRYELAAALNRLPTF
                     RVSHATE"
     gene            12497..12943
                     /gene="dtd"
                     /locus_tag="PKI68_04155"
     CDS             12497..12943
                     /gene="dtd"
                     /locus_tag="PKI68_04155"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNX52916.1"
                     /translation="MKLIIQRVKSASVKVDGKTVGDIGSGLLVLAGVSHGDTVADAAF
                     LAKKTVNLRIFDDENGVMNRSVLDTGGAILAVSQFTLYGDCKKGNRPSYIEAAPPEKG
                     LKLYEEYVKQLRELGPHVETGIFQADMEVSLINHGPVTLILESTGR"
     gene            12940..>13261
                     /locus_tag="PKI68_04160"
     CDS             12940..>13261
                     /locus_tag="PKI68_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52917.1"
                     /translation="MKTVSKWKNEKPQIPTAAVWLLNDLAEYRGKQELFTKQAPQKLK
                     ALREHALIESAVSSNRIEGVEVDQKRIGTVLFGKGHLQDRDEEEVRGYQKALSWIHEK
                     GAKIP"
BASE COUNT         2682 a         3437 c         3713 g         3329 t
ORIGIN      
        1 gacttctacc gtgtgaaggt agcgctctaa ccaactgagc taaacgccct caaaaaaaga
       61 ggcttatctt ttacttgccg aagaactgtt cgtaaaggat ttcttttatc tgatcgagac
      121 cttcaccgag tccggcggaa atctcgaccg gtttcaggcg ggttttgcgc cggaaaattt
      181 tgatgttttc cgccgcggcg ggcatgtcca ttttgttcgc cagtacgagc gacggacggt
      241 tggccaggtc cgcgttatag gctttcagtt cgcggcgcag gctgcggtag tcttcaacgg
      301 ggtcgcgtcc gtcttctgcg gccatgtcga tgacgaacag caggaagcgg gagcgttcga
      361 tgtggcgcag gaagtagtgg ccgaggccga ttccttcgct ggcgccgtcg atgagtccgg
      421 gaatgtcggc gatccggatc tggttgaagt tttcgtagat gagtgtgcca aggatcggat
      481 tgagtgtggt gaagggatag gaagcaattt tggggtgggc gtcagtaagt tcggtgagga
      541 gagtggattt tcctgcattg gggtagccga ccagaccgac gtcagccacg agtttcagct
      601 caagacgata agcgcgttct tcgccgggcg ttccgggtgt acatttggtc ggagcctgat
      661 tgacggatga tttgaagtgg ggatttccga ggcctccgcg tccgccccgt gcaatgacca
      721 gctcctgtcc gtcttcgaga atttcgccga cgaattcctc tgtatcgggg tcgaggacga
      781 cggttccgca gggtacggtg agaattctgt cttctccgca gcgtccggtg cagtcgttac
      841 ctttgccgtg gccgccgttt tctgcattct ggtgggggtg atagtagagc gcgttgagtg
      901 aatccagatt gaagtctccg cgcagaatga cgcttccgcc gtggccgcca tcgccgccgt
      961 tgggtccgcc gaactgggcg aatttttcgc gccggaagga agcaattccg ttgccgccgt
     1021 tgccggcctg cacgaaaatt ttgacgcggt ctctaaatac gatggttttc acggcgggta
     1081 ccttaaatga agcaggcgag ctctgaaagc tcgccttctc tttttccaag cattggaaaa
     1141 cggttatgca atctgcattt cgcggatgtt aacgcggcgg ccggctttat cgaactcgac
     1201 gataccgtgt ttgagcgcga agagcgtgaa gtcgcggccc tggccgacat tggctccggc
     1261 gacgaacttg ttgccgacct gccggacgat gatgctgcct gcggttactt cctggccggc
     1321 gtagcgttta acgccgcggc gtttcggctg gctgtcacga ccgtttgttg aagacccttg
     1381 tcctttttta tgtgccattg ttctgctcct tcaaaattaa gcggtgatgg attcaatctt
     1441 tattttggtc agtccctgac ggtgaccgat tttccggcgg taaccctggc ggcgtttctt
     1501 tttgaacgat accactttgt cgccgcggta gttttcgacc acggtggcag tcactttagc
     1561 gccggccacg acgggagcgc cgatggtgag ttcgctgccg ttggaaacgg ccagcacctg
     1621 atcaaattcg atcgctttac cggcttcagc ggcgtccagc agttcgacgc tcagaacggt
     1681 atccttttca acgcggtact gtttaccgcc ggtttcaatt actgcgtatg cttccataac
     1741 aaattccttt ttgggtcaaa gacgggagaa ggtaggccag aaggcggggg ctgtcaacac
     1801 cttatagctc atcttttacg ggctgttttg caggcggcgg ctctttggct tttctggtgc
     1861 cgtttgtata gagcgactct gctccgacag acggtgcgct gagtttcaga ccgccgcgcg
     1921 ctgcttcggg aaccggattg ggcggggtat agagataggc tcccgtcggg tcagctaccg
     1981 ggaagctgaa ggttgagaaa atcaatacgg agggcgatgg tgagccgtat ggttttttgg
     2041 ttgaactgta ccgaaggcta ttagacagta aaagaatgac taagccactt gcaacgacat
     2101 tgtgtccgct ttgcgtcgat ttagacggta cgctggttaa aactgacacc tttgcgcagg
     2161 aaatgctgct tcttattcgt actcgcccgc ttacgttttt ttatcttctc cgtcggatat
     2221 ctgctggact ggctccattt aagcagcgga tcgcaggtga gattcaactt gatcctgccg
     2281 cgctgccgta tcaattcgaa ttccttgagc ttctcaaaga ggaatatcgg acggggcggg
     2341 aactgatttt agtgacggct tcggacaaag ccccggcgca agctgtggcg gaatatctcg
     2401 gcttgttcag cgatgtcatg gcaagtaacg gggtctgtaa tctaaaagct actcgcaagc
     2461 gcgatgcttt ggttgcccgt ttcggcgaaa aaggcttcga ctatgccggt aatgctgtag
     2521 atgaccttcc ggtctgggcg gtcgcgagga acattattgc agtaaaccct tcgacaacgg
     2581 ttctccgcgc gcttgcaaat cgctctgctc gggtatttga agaccgtccg ccattatgga
     2641 gagtttggct gcgtgcgcta cgtgtgcatc agtggacgaa gaacatgttg atttttctgc
     2701 cgatgctgct ggcgcatgag ctaacgaatc cggtgcttta tctgcaagcg gtgattgcgt
     2761 ttttctcgtt cagtctggcc gcttcgacga tctatatatt caatgatata ttcgatttgc
     2821 atgccgatca gcaccaccca cataaaaaaa agcgtccgtt tgcctcaggc aatctaggtc
     2881 ttgggggcgc tgttgtggtt atgccgttat tgctgatttc atttttgttg gccgcacttt
     2941 tatctccgac atttatcggg attcttttgc tctatttggc gcttaccaca ctctattcct
     3001 ggtggctcaa gcaaactgct gttcttgacg tgttgacgct cgccgggctt tatgcgttac
     3061 gcatttttgc cggaactgcc gcgtatggag tggtggcttc tgagtggctg attgcttttt
     3121 caatttttat tttcctcagc ttggctctgg ttaagcgtta tgcggaactg agagcggcag
     3181 ctcaacagat gccggactct cttcagtctc gcgggcgggg gtaccgtgtt gtggatatgc
     3241 cattgttagg tggtttcgga gttgcaaccg gatgtctctc tgtgcttgtg ctggcgcttt
     3301 atatcagcag tcctagagtg attgattttt atccccgtcc tgccgttctc tggcttctct
     3361 gtcctcttca gttctactgg attgctcgca tctggctgtt ggccgggcgc ggcgaaatgt
     3421 ctgatgaccc tctcgacttt gctgcccgcg acctgcgcac ctggctggtt gtttttttaa
     3481 gttcgatgac gttagtcctc ggcagctttt agcaaacgtc aagtgatcct ttagatttta
     3541 cgaacgtgta aaatgcttaa aagcgatggt ttgaatcgaa catgaatact ataaaaaaga
     3601 ttctcaattc gtttagaata gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 ntggtttgaa tcgaacatga atactataaa aaagattctc aattcgttta gaatagatca
     3781 atgctcatgg gcgccggaac aggaacctaa aaaattccat gtttttgctc tattgcttat
     3841 ttttttgttg ttttcgggtt tgtacggtct gctgggctgg ctgtttatgc ggctcggttg
     3901 cttttctcat gttgatattt tttttggcgc agatgcccgt tggatggtcg gggatatcgc
     3961 cgaatttgcc gcgaatcact atcgtacaaa tacgcatccg ctgggagttt tgcttgttaa
     4021 tccggtcggc actctgctga acctgcttgt gcgatctccc caaacgacag cattgttgat
     4081 tacagcgtta accggcggtg gcttcgttgt attgcttgtc gtttttcttt tgcggctttg
     4141 catttcaaaa actctggctg ttctctgtgc ttttttaggc ggcctgtcgg cctgtaatct
     4201 gttgtggagt gcggttcctg attatttttt attagatggc atctctcttc tttttttctg
     4261 ttgggttgta acagcgcatc cgcacaagtt gtggcttgtc gttgtctcgg ccgcgattgc
     4321 agcaggtact gtgacaacca accttcctgt ggtagtcatg atctacactg tggcccggca
     4381 cgggatccgg cagaaactct cggtcatgct gcgggacggc ataaagtttt ttgtgctgat
     4441 tttggcgctg gtgacgatgc tggcggtagt acaggatctg atctatccct catcaacact
     4501 tttctttcgg acacatgctc tgcagagccg tactatgtat atgagcagtg aaagtacgat
     4561 tggccgtttt gcactgtttg tgccctatct gtttttgttt aacattctct ttccaagcgt
     4621 tgcgcgggtc tccttggggg cggaggggct ggccggcata cgctttccga ttacaaagct
     4681 gtcggcatgc acagagctgt ttacgccgat gggatggctt tttgttttgt tgtggctcgt
     4741 gctactggga ttttcccttt gggtatgtat cagagaaaaa caaatggcta agccgcttac
     4801 ggttcttttg gcgttgtgcc ttttgtttaa tgtcgcctta catttgtttt atggacggcc
     4861 agatgaattt tttctgtata ccccgcactg gacttttatg gttatagctc tgctggggat
     4921 ctcattggat gtcatttccc ggaaattcag acaggtgttc cttgctattg tgatctacct
     4981 gcttttactc gttgtccttt taattgcaaa caatgcgacc gtgatccgca agatacttga
     5041 tattcttgaa tgagtcctat tgctcatctt ttgcgggcgg cggctctttg gcttttctgg
     5101 tgccgttcgt atagagcgac tccgctccga cgggcggcgc agtaagcttc aggccgccgc
     5161 gcgaagcttc ggggatcggg tctggcggag tgtaaagata agcgcctgcc ggatcagcga
     5221 ttggcagtct gaaggttgga aagtcaatgc tgggggccaa ttcaaccgac agagtttcga
     5281 atttttgagg catcaagatc tgggtcacaa acagacgagt aggggaatag ccttggccaa
     5341 gattggcctg atggtagatc acgggcgaat aataaagtcg gctttgggcg gagcgtgttg
     5401 ttgtgccgac ccttcgtcga gggctttggg ttagggcggc ttttggcttt tccggctcat
     5461 cgggactgct cggactgtgt ttgcgctgct cgatctgtga aagttttccc gccggcgatg
     5521 cttcctgttt ctgccccggt gtggcaatga ttccggtctg cagagccttc tgctcctcac
     5581 gcttttcttt cgaaagctga cgaaacgcaa catcttcttc ccgttccggt tcggggcggg
     5641 cggtgaatcc gcgtttagcc agatagctgg cgtccatttt ggacggatcg agcaggttga
     5701 aggtaaacgc cacaacgccg agataaatca gcatattggt gagcatcacg aacgcgcacc
     5761 cggtctggag cgtcgaaaac ggtcccttct gctcggtatt ctgctgtagg ttctctgtca
     5821 tgaccgcccg ggttcaacgc ctgattcata aggaacgacc ggatcgtagc cgttagaggg
     5881 ccgaaaggca aacttagcgt tcaatccggt ttacgcgcag ggggtagcgt attttcaggt
     5941 cgcctccgag caggatttcc acccagtacg tgccggcttc cggaaacgtg atgttgataa
     6001 atacttcgac gctggtggcc gcggcttccg gcgaaggaag ctgtacctga acgtcctgtc
     6061 cctcagccag cacggttttc tggtcgggat ggataatccg cgagcgctgc gtaaacgttc
     6121 cttcaccgct gcaccagcgg gtgacgaggc acattctggc gaaagcgacc ggcaggcgct
     6181 cggcatgaat ggcgtcaaaa agtccgatca gcatgaattt gccgttacgt tcctgacgga
     6241 cgtcatcgca gatcaggctg gactgaaggt cgggaatcat catttttcca ccttttggag
     6301 ttcctcggaa cggtcctttg ataagtccag cgttccgaac tgttcctgat cgggaagtgc
     6361 catatagaag cggttgcagg aatcgagttt cagttcaccg ccggtagtgg tgaagctgag
     6421 aatatgaccg cctttggttc tgtcatctga aataaagtgc aggtgccagc cgggaacatt
     6481 gacgccttta acaaatgaag ggcagcggaa ccccagtacg gtgccgttga cactcgcata
     6541 ttcagcgact gtctgatgct gtacaacttc agcaagcggc ggatatggtt tctcctgttt
     6601 cggcaccgag cggactttca ttaagggaaa gtggccgtcg aaccggacgg caacgaaaag
     6661 gtttgagttg ggcgcgaggc cgtccgcaaa atcctgaaac atcttttcgt cgagcgcgcc
     6721 atcatagtgg cagatcgaat cggctttaaa attcacgacg ctggcaaacg gtgtagtcgt
     6781 attggttggc atcaggcaga cgcttccgtc ggcgcgcgcc tggtaaacct gacggtcaat
     6841 tacgatcatt tcgccttcga gtccgtcgaa cgtgccgatg ccgaggctgc cgtagttcag
     6901 caggtttccg gttgtcatct gcccgtcgta agcgcctgcc agcagggcat cgatggtgga
     6961 aatctgtgtc agcgtatttt tcggagcggt ggcgcatccg gccagcagcg gcagcaggag
     7021 cagcaggaac tgttttctca cttgaatacc tccgttaagg tttcactttc gggtgtcata
     7081 tcgttctgga gcaggttgag cagttttttc gccaccggtc cgggttttcc gctgccgacc
     7141 ggccggcctt cccattcgat gaccggcgta acgtcggtgg tggtgccgag gatgaaaatc
     7201 tctttcgcct gcgcggcctg cgccggactg atgtccctgt attcagccgc tttcagtgtg
     7261 ccgtcagccg tcagcgcttt cgccagatcc agcgtgcggc gcgccgtggt tccgggaagc
     7321 acatagtccg gcttcggcat cagcagttca ttatccggcg tcagaatgcc gacgttttcc
     7381 gtggcgcctt cgccgaggtt gccgttggca tcaacggaaa tgacgaaatc gagtccgcgc
     7441 tggttggctt ccagcttcat caaaacgttg ggcagatagt tacaggtttt gatcgtcgca
     7501 aattccggcg gtttaaccgg gactttgctc agcgcgaccc gcgccggttt cagtcggccg
     7561 ttttcaattc gcgcagtgta gcggtagaca atcacgtaaa ggaccggccc tttgcagagc
     7621 ttcggatcaa tgcccatatt ttctgtgccg cgcgaaacca gaacgcggat caggcagtcg
     7681 cgttttccac cggcgcgaat cgtgtcgcag ataatctgag tcagttccgc gcgtgtgcag
     7741 ggtgcttcca tgccgatgct tttgcaggag agttcgagcc gtgccagatg tgcgtcggca
     7801 ttatagagcc gtccgttcag gcatttgagc gattcaaaaa cgccgtcgcc gcgctgcggc
     7861 aggtggtcgt ccgccggaat ggtcatcagt gccggatcaa gggtgatgcc gccggtcagt
     7921 ccggagtaca tcgcatagag agtatttgaa aggttgccgc gcgcgtcacc gagccgtgcg
     7981 agccattcgc tgttcgtcag ggtctggagc tgtttcatgg gaaaaaccat acgctaaaga
     8041 ttccaagatt gggaagctta tgttatgttc cgtgcgttca gtgaatggag atttatccga
     8101 tggtaatgga aacggcattt gcaccggcag agcgcgcctc tcaggaggaa atccgccggc
     8161 agtatctgaa actcgcgcaa ctcccgtttg tccgcgactt tctcgatgcg attccgaata
     8221 tgtcggtggt tctgaatgaa caccggcaga ttgtttttgc caaccgcgcg ttcgcggagt
     8281 ttcttggcat aaagagttcc gatgaactgc tgggtaagaa tcattgtgaa gcgttcagct
     8341 gtctctatgc tgacgttctc ggcgcccgtc ccggcgaggc cgtcggctgt gttcgttcac
     8401 gtttgactga aggtggttgc ggcacgacga tgttctgccg cacctgcggt gctgtgattg
     8461 ccattatgaa cagccagaag ctgcgcaagc tggacattca ggagtgccgg atgatctgcg
     8521 gtgaagaagg cttgaatgaa accgcgctgg atctgcgggt ctggtcgcgg ccgattgaag
     8581 tgagcggcga agtgttcacc gtgttctcgg tcgtggatat cagtaacgaa aagcggcgca
     8641 aggtgctgga gcggattttc ttccacgatg tactgaatac ggcaggcgga gtcaaagggc
     8701 tggccgacct gctgattcag acggagctgt cagaggtgga aatcaaagat atttccagca
     8761 tgatttctga gtcagccgac catttgatcg aagagatcag cgatcagcgg atactttccg
     8821 cggcggaaag cggcgatctg gaagtctcgg tgcaggaagt tcactcgctg gaagtgctgt
     8881 gccggatcat ccgccagttc cattcgtaca gcattgccaa aggaaaggtt cttgaggtgg
     8941 atgccgcgac agaacatttc gactttgtga ccgatccggt actgttgcgg cgggtgctga
     9001 ttaatctggt gaaaaatgcg ctggaggcga ttgatgaggg cggaacagtt acgctcggtt
     9061 catacacaga cagggattcg gtttatttta ccgtgcatga tgcggccgcg attccgccgg
     9121 aagttcagct gcaggtcttt tcacgctcat ttacgacgaa ggggagcggg cgcgggctgg
     9181 gcacctacag catcaagctg atcagcgaaa aatatctgcg cggtcgtgtt tcgtttgttt
     9241 cgaacgccga ggagggaacg cggttcacca tccgttaccc gaaggtgatt caatcgtccg
     9301 gagaattgga tgaaaccgcc ggttgaaatt atctgcacac gctgcggcgc ggaagccttt
     9361 cttaagcgcg agcccgtcta tgaaggcttt acgaaaaccg gcgaagagct gagctgttcc
     9421 gcctgcggat tcatctttgc tgccgaatcg gaagtcccgt tcaaggcccg ctccgccgca
     9481 ccgaagattt ttaccgatgc cgaccggtca gcatcggtga aagttttcag cgaggacgaa
     9541 aacaaatgca tctgtcgtta ctgtgcaaac tatgtggtga atccgtttgt gcagttctgc
     9601 gcgcgctata agaaagaagt gcaggcgacg gacagctgcc ctcagttttc acctgtgcag
     9661 aaaaagccgg gcaaaccgct attttagtaa gtccgtccgg cgataaactc cgccagctgt
     9721 ccgagccggg ccggattttt aagcccttgc agcgccttga ccgcatcagc cgtcagctgt
     9781 ttcgcatcgg cagcggtttt ctcggttcca tgaacagccg gccaggtcat cttgccgcgc
     9841 tctttgtctg aaccaatatc ttttcccagt tcttcaaccg ttgcgctctc gtcgagcagg
     9901 tcatcggcca gctggaatgc cagcccggta ttttctccgt atatggccag gtgtttcaat
     9961 gtctgtaaat ccgctccggc gcacatgccg cccatcacac acgcggcgcg gatcaaggca
    10021 gcggttttgt tgcggtgaat atattctact aaagcagcgt ccggcgcttt accttcagca
    10081 tcaatatctt ctgcctgacc gccgattaca ccgcggctgc ctgcggcccg ggcaagttcc
    10141 agcgccagcg cgccgccttg aggctgcccg gccagcactt cgaaagcgag cgtcagcaga
    10201 gcgtcgcccg ccagaatagc cgttgcttcg tcgaaagcga tgtggcaggt gggccgtccg
    10261 cgccgtaacg tgtcatcatc cattgcgggc aggtcatcgt ggatcaaggt atacgtatgg
    10321 agtaattcca gcgcgcaggc cgctggcatt gcctgttccg ctgtaccgcc gcacgcttca
    10381 caggcggcga tgcaaagggc gggacgcaac cgtttgccgc cggcaaagat actgtaccgc
    10441 atggcgttgt gcagttttaa agggcgttcc gtctctggag gaagcaaagc atcaagcgcc
    10501 gcattgacgg cttcggactg ccgtttcaag tggttttgca gatcgttcat cccgccgatg
    10561 atagctcaaa gcccttatta gtaaagtaat agcagggtag aatggtttga cagcggcgga
    10621 gggcaattct actatctctg ctcattttgc agatgggaac tgttaaccac caaaacgggg
    10681 aagcttcgct atgaaagaca gaaaagagtt ttacaacctg ctcgctgaaa tcgacggaaa
    10741 gccctttgct gaatatgaac ggctgctcgg cgacttcgac ttcgcccgct atgtcatcaa
    10801 atgcacgaaa atcggcaccg ctaacggcgc tgaaaatccg gtttttaaca tccgcattcc
    10861 gcaatccatc gccgaactgc cgggacacct ctacgactct ccggtccgcc gtacggcact
    10921 cgaagacttc ctgacccgta atgttgcgga atccgccgga cgtatcgccc gtttcaacga
    10981 aagcggttgg gcccgccgca acatcatcgt cgccgaaccc ggccagaaga ttctgccgcg
    11041 cacctcggtg gtggtcacca aggaatacat cgacgtccgt ctccgcattg cgctgcccta
    11101 caaagtctac gctgccggcg aacatctcat tgacggcgaa atggcccgca aggtgttttt
    11161 tgatgacctc cccgaagttg tcagttccag tattttcttc tgcaacatcg acctcgatga
    11221 agctgagagc tttgtgaacg gcatggaaga cgccgaccgc gtccgccaga cgctctcaac
    11281 gcttggtttc gtcagttttg tcggcgaagg ttcactgctg gcccgtgagc aggacagtga
    11341 tctgcctgat tacgaccata tcgcgccctt cgaagtttcc gaagcagctc agaccgaaat
    11401 cgatgtaccg aacgcaggaa aaatcatcgg ccttggtatt cctgccggat tgacgattgt
    11461 tctcggcaat gccgccaacg gccgcaaaga tttcatgtcc gctatcgccg ccggtgtgtt
    11521 caatcacatt cccggcgacg gtcgcgaaac cgttgtaacc gtttccgatg ccgtccagat
    11581 tgccgccgac cgcggccgca gtgtgcagga agtcaacata actccgttcc tctccgagtc
    11641 cgaaaccggc aacccggcct cctacagcac ccggtccgcc gactccttca tctcgcaggc
    11701 cgccgccacg atcgaagccc tcgaagtcgg agcccgggta ctgatcgttg atgaaaacac
    11761 ttccgcgcct gcctttctga ccacggatgc ccgtatagcc ggactgctcg gcaaaacgcc
    11821 gcgcgtttcg ctggcacagc gcgcccgcca gatggtggac gaactcggga tctcgctggt
    11881 catcggcggt gaaaacctgg tggctgaata tatcccgttc gccaacacgg ttctgaaagt
    11941 cgaagacttc caggtcacca acatcaccga agaggcgaaa gctctcaacc tgccggttcc
    12001 gccggaagcg ccggtcgtga atctcggttc gatgctcgga cgttcgcgct ggatcatgcc
    12061 gagcagtatc gacgctgctg tcggaagcat tgatcaggtc atcgaagcca tcgacctcaa
    12121 agccattcag ttcggacgct cagtgattga actcgacagc gttccgcaga ttgctgacgt
    12181 aaaccagacc ctcaccatcg gcctgcttct ttactacgcc aaactgcgct acatgcagga
    12241 aggctacccg ctgcgcgaaa tgctcgacat gatcgaccgc gatctcagca gcgaaggcct
    12301 cggcacgatc gcccgcgacc tgcgcggcga tctcgcccgt ccgcgccgct acgaactggc
    12361 cgccgcgctc aaccgcctgc cgactttccg cgtctcgcat gcgacagaat aagaggacgg
    12421 tcaaacgtca aatgtctgaa ggtcgaaagt cgcggattat tcctgacttt cgacctttga
    12481 ctttaagacc ttcggcatga agcttattat ccaacgtgta aaatccgcgt ccgtaaaagt
    12541 tgacgggaaa accgtcggcg acatcggaag cggccttctc gttttagccg gcgtgtcgca
    12601 tggcgacacc gtcgccgatg ccgccttcct cgccaaaaaa accgtcaacc tgcgtatctt
    12661 tgacgacgaa aacggcgtca tgaaccgctc tgtcctcgac accggcggcg cgattctcgc
    12721 cgtcagccag ttcacgcttt acggtgactg caaaaagggc aaccgtccca gctacatcga
    12781 agccgctccg ccggagaaag gcttgaagct ctacgaagaa tatgtcaaac agctccgcga
    12841 actcggcccg cacgttgaaa ccggcatctt ccaggccgac atggaagtca gccttatcaa
    12901 ccacggcccg gttacactga ttctcgaaag cacaggacga tgaaaaccgt ttccaaatgg
    12961 aaaaatgaga aacctcagat accgacggcg gcggtgtggc ttctcaatga tctggccgaa
    13021 tatcgcggca aacaggaact ttttaccaag caggctcctc aaaaactcaa ggcgctccgc
    13081 gaacacgcgc tgatcgaaag cgcggtttcc tccaaccgga ttgaaggcgt ggaagttgat
    13141 cagaaacgga tcggaaccgt gctgttcggt aaaggacact tgcaggatcg cgatgaagag
    13201 gaagtccgcg gctatcagaa agcgttgagc tggattcacg agaaaggggc gaaaattcct
    13261 c
//
LOCUS       DAOPZO010000041        13139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_5221_length_13139_cov_10.493198, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000041 DAOPZO010000000
VERSION     DAOPZO010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 13139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13139
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5221_length_13139_cov_10.493198"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PKI68_04165"
     CDS             complement(<1..359)
                     /locus_tag="PKI68_04165"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction ATP-dependent DNA helicase
                     RuvA"
                     /protein_id="HNX52918.1"
                     /translation="MITFLEGILEEKQPGRAVMNVGGVGYEVLVPLSSFDRLPPEGAK
                     TRLLIYHYITEVSQVLFGFATDDERKMFTLLLGVSGVGPKIAVGALSGLSVRELKTAL
                     IESDVKKLSSISGIGKKT"
     gene            698..1663
                     /gene="sppA"
                     /locus_tag="PKI68_04170"
     CDS             698..1663
                     /gene="sppA"
                     /locus_tag="PKI68_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNX52919.1"
                     /translation="MNRHRNSRKPWIITALLAIALLGTLLSHCRQVEKDYPRDEAPKL
                     DETWSSGSGNIKVARIPLNGVIMRAEEEGFFAARSDMVESILTQIRAAGRDPDMRAII
                     LEVNSPGGGVTPSDEIYNALRGFRNSRPDRRVVVFIRDLGASGAYYAAMAGDFIIAEP
                     TAVVGSIGVIMQTMNFQGLSEKIGITDVTIKSGANKDMLNPFQPVNTNQVALLQKLID
                     EMQTRFSSIVIEGRKLDPAAASGLFDGRIFSAADALNKGLIDQIGYWNDALARTEKLL
                     DAGTLRVIRYEPQRTFFEELLEGKSPVVLPNLKLHSPQFLYLWKP"
     gene            1691..2200
                     /locus_tag="PKI68_04175"
     CDS             1691..2200
                     /locus_tag="PKI68_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNX52920.1"
                     /translation="MKKTVFALLSAGLLSGCETMPFQIQTPATRAAVTEDQLIAQENQ
                     RKMAGRLETLELQMNQMSRDLDTLKGQLSTRCAAIEQKSEADKREMVARLSGELDKLM
                     KLASAPVPAPKPPAATSGIEHVVRSGETLYIIAKAYNVSTKTIIDANRLTDASKISVG
                     QKLLIPGAK"
     gene            complement(2213..>2956)
                     /locus_tag="PKI68_04180"
     CDS             complement(2213..>2956)
                     /locus_tag="PKI68_04180"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNX52921.1"
                     /translation="SEPNHAGGKFPRPCVHERLGRALEKVRPQVVIACYGMNDGIYAP
                     PDEERMRAFQNGITRLVQDCRAMGAQVILVTPPIFEGGNYRSASSADAKPPVYEGGYN
                     TVLDQFAAWEKEHPPEGVAAVIDLHSFMAAARAERQKTVPDFKFTRDNIHPKELGHLV
                     MAQAILQALQVPVPDAPAEELLKSAQADPLFALVDQHRTLCSKAWLMHVGYVRERTVA
                     PNSGDLAADEAKAAVLQREIDTLRRRGKQ"
     assembly_gap    2957..3056
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3059..3145)
                     /locus_tag="PKI68_04185"
     tRNA            complement(3059..3145)
                     /locus_tag="PKI68_04185"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3109..3111),aa:Leu,seq:gag)
     gene            3250..3849
                     /locus_tag="PKI68_04190"
     CDS             3250..3849
                     /locus_tag="PKI68_04190"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: protein motif:HMM:NF011397.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HNX52922.1"
                     /translation="MKLVIATRNRHKLEEIHAIFNFAGLEVCSAFDFPEIPDVVEDGA
                     TLEANAIKKASEICRATGLPALADDSGLEVEALNGAPGIYSARWSGEGCTYHDNNVKL
                     LRELSGKENRRARFRTVIALALPGETPQTVEGSVQGWIIDKMRGDQGFGYDPLFVPDG
                     YNQTFAELPADVKNRISHRARALNAASNIWKHFFQGLEA"
     gene            complement(3900..4241)
                     /locus_tag="PKI68_04195"
     CDS             complement(3900..4241)
                     /locus_tag="PKI68_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNX52923.1"
                     /translation="MKYIVAIIQPDRLDPVLDALEEKEIHLVTVSTVMGRGRQKGIAA
                     VYRSHKEAGSLLKKVKLEIVVNDDFVKPALDAVTQGARTGEIGDGKIFVLNLEECMRI
                     RTGETGHVAIG"
     gene            complement(4259..5569)
                     /locus_tag="PKI68_04200"
     CDS             complement(4259..5569)
                     /locus_tag="PKI68_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNX52924.1"
                     /translation="MLAGNGFGQDVVAGALSTPAPAPAINAGDTAWMLISAALVFLMI
                     PGLAFFYGGLVRKKNVLSILMQCFAVICLITLQWALCGYSLAFGPDVKGWIGNLDWAF
                     LKGVGVDPFTGYAATIPHSVFMIFQAMFAIITPALILGAFAERMKFSAFCVFMLLWST
                     LVYDPVAHWVWGLGGFLSRFDAAGNAVPGALDFAGGTVVHINAGVAALVAALILGKRV
                     GSVSPPHNLPFAVLGAGMLWFGWFGFNAGSALGANGLAGSAFVATHIAAAVAGLTWAV
                     CDWLWHKKATTLGMITGAVAGLVAITPACGFVSPMSAIWIGAGASIICFIAVTFIKAV
                     FKYDDTLDAFGVHGVGGIWGALATGIWAEKSVGGFDGLLHGNPGQFLIQLKAVAITMA
                     YSLVVSFVLLKLVDKFMGLRVSEHEERVGLDLTQHKEAGYTTID"
     gene            complement(5809..8067)
                     /gene="ptsP"
                     /locus_tag="PKI68_04205"
     CDS             complement(5809..8067)
                     /gene="ptsP"
                     /locus_tag="PKI68_04205"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNX52925.1"
                     /translation="MGKANVNMICDIAELSSLFEKSSSLDDFLQSAVSVVAWHMKAAV
                     CSIYIYDDKERMLTLRATQGLSPDAIGRVTLRLGQGLTGLALKELRAIREGRGVLSPK
                     FQPVPGINEERYQAFLAVPIRRGLTRVGVLTVQDVQPDYFDEKDEQALKAIAAQLATV
                     IENANLLLTLHRQKNFAKPDEVRTLPKMIKGRPASGGVAQGRTAIIISGRGGSFQVPE
                     GAPQNLTVQDFDRALQLTEQQLKDLQRRADEELSDVAQLIFNAHLLILADEEFSGEMR
                     RRIVAGTPPAEALVALVNSYIEIFSASPNLRLQEKVHDLKDLGHRLLENLIGGEHERG
                     DYSGQIVVADALLPSDILKLATENAEGFVVQGGVTSHNAIICRSLQKPMVMIDPQQLE
                     LLGKPCELLIDGNQGSIFIQPSEEVLKEYNEYRRAAKALETAPPVKAETRTADGTRVQ
                     LFANINLLSDLKLALSFHTEGVGLYRSEFPFIIRNAFPSEEEQYQVYNRIVESMGGRE
                     VTFRTLDIGGDKVLTYQNEAEEANPFLGLRAIRFSLLHKDLFAEQLRAFLRAGAGGNT
                     RIMFPLIASLDDFIEARAVVYDCMKELDARGVPYNRNTKIGIMVELPSAVALIEDLAH
                     EVDFMSIGTNDLIQYMLAVDRTNEMVSKLYTSHHPAILRAIERVASVARSHRKDVSIC
                     GDIAMDPKMIPFLLGAGITKLSLPPGRVHEIQQVIEKISMEKAVYTARRMLSASRISE
                     VDAIINGYNFAS"
     gene            complement(8137..>9265)
                     /locus_tag="PKI68_04210"
     CDS             complement(8137..>9265)
                     /locus_tag="PKI68_04210"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX52926.1"
                     /translation="GIPGTARLLGGKESAERDFADCVRLPSFEACQADKQAVLELTRL
                     TEREQNPFSGKPLVQFHGARAVLIERPAAPLSAAEMDRVYELPYMRLPHPSYKEPIPA
                     FTMIKESVTVVRGCGGGCAFCGLGLHQGRFLTSRSEESVVREVKTLAAMPSFSGTITD
                     LGGPTANLYGCVNGVCPACKVCRRASCLYPKICPNLELDAGSAIHLMKRVRELARVKH
                     VFIQSGIRMEVALRNPHYIRELAKHHVSGHLKVAPEHMHPNVLKKMRKPGPETFCEFQ
                     KAFEAESRAAGKKQYLVPYFISNFPGSTEAEMKAVEKFVREEEWKLQQVQDFIPLPMT
                     PAAAVYYTGLDYETGEPVPVIRGLAERRTQMKQLRPHTKHR"
     assembly_gap    9266..9365
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9366..9880)
                     /locus_tag="PKI68_04215"
     CDS             complement(<9366..9880)
                     /locus_tag="PKI68_04215"
                     /inference="COORDINATES: protein motif:HMM:NF020086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52927.1"
                     /translation="MTRAEMKARGWDELDVLIVTGDAYVDHPSFGAAVIGRVLEADGW
                     RVGIIAQPDWTKPESLTVLGRPRLFCGITAGNLDSMLSHYTAARRKRKEDSYTPGGVA
                     GKRPNLAGMVYTQLAKKAFPGMTTVLGGIEASLRRTAHYDYWQDKLRPSILQDSKADL
                     LVYGMGERAIR"
     gene            10006..>13139
                     /locus_tag="PKI68_04220"
     CDS             10006..>13139
                     /locus_tag="PKI68_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNX52928.1"
                     /translation="MQKLDTQLFLGLYRHMLASRKADAVQEDAAQRGEAFFYIPSSGH
                     EAMAALAPHLTPTDWLHCHYRDRALMLARGVTQQDLLLDLLGKTGSPTEGRNMPGFAC
                     NRALHLFGAPTGVGSNALQAVGAAQAIKDSRDLVYCGIGDGGTQEGEFFEAVAEAVRS
                     QLPVLFVVQNNRYALSTPSKGRTFFSLPEGDAKEFYGLPILRANGTDAVETYRVFGEA
                     VAKIRQTRGPQLVVVDMERLTSHTNADDHTLYRSADDIREMHEKADPILILAEKLIAA
                     GIPEAQLKAIEHETNQQLTAAFDAARKAKNAKTELSAKKPLPARLTDAASEKRTAGDA
                     LTMLEAMRAVLRARLGEDKTIYLYGEDLEDPKGDVFGLTRGLSTAFPGQIINAPLSEA
                     TIVGAAIGQAMVGKKPVACIQFADFMLPAFNQIAAELGAMWWRTNGQWECPVILMAIC
                     GAYRPGLGPYHAQTFDATFAHIPGLDVFMPSTAPDAAGLLNAAFESGRPTVFLFPKNL
                     INDRSVTAAADAATQFVPVGKARVTRPGRDLTIVSWGSTVPLCEKTADALQNAGASVE
                     VIDLRSLVPWDQETVIASAQKTGKLLVVHEDNHTCGFGGEVMATVAEKANVPVQMARV
                     ARHDTYIPYLFETQIEVLPSFRTVLGKAAELLDYSLTWQKPVEEAEGSVTVNAIGSSP
                     SDKTVKVVELHVKAGQPVQAGELLASVEADKATMEISSPVGGIVEALFLAEGDSVDVG
                     TPLARITTEEISLIKKPVTKDDPGTPVLEKKLSAAKAKITAVEHKPVLISSIATVLGS
                     RRILNEELLKGHGEWDSEAIRSRTGCEERYWITGDENVETLAVKAVRDLLEMEKLTLA
                     DIDAIVCSTGTPLMMTPSLACRVLKELSPEKGEVQMQAHDVSAACAGYMYALQNAVDI
                     LRDDPSKKVIIITAETLSPMINHEDQKTLALFGDAATASLLSCEPRGGNVNVLVNRPV
                     LSAVGVDQKVLYVPHMGTGEKIEMEGLTVFKIAVRKMIDMLAEACEMRGVAVDALDKI
                     VPHQANERIIEAIR"
BASE COUNT         2740 a         4065 c         3687 g         2447 t
ORIGIN      
        1 gtttttttgc cgatgccgga aatcgaagag agttttttca cgtcgctttc gatcagcgcg
       61 gttttgagtt cgcgcacgga aagtccggag agtgcgccga cggcgatttt cgggccgacg
      121 ccgctgacgc cgagcagcag ggtaaacatt ttgcgttcat cgtcggtggc aaagccgaag
      181 agcacttgcg agacttcggt gatgtagtga tagatcagca gacgggtttt tgcgccttcc
      241 ggcggcaggc ggtcgaagct gctgagcgga accagcacct cgtaaccgac gccgccgacg
      301 ttcatgaccg cacggcccgg ctgcttttct tcgagaatgc cttcaaggaa agtaatcatg
      361 ggcgggagcc tatcagagcg cctccgaagt atgaactatg aaatatgaaa gatgaaaaag
      421 gttttggacg atccgttaaa cagaaaagcc ccgcagaaac aggttctgcg aggttattca
      481 gttccagaag ccggaagaaa ttacttcttc ttggcagctg cagcttcgcg agcgcggact
      541 ttcaggcgtt ttttgcgaag tacacggcgg cgctgtttgg tgcgttcttt aagttgttgt
      601 cccatagtgg ggagagcttg taaccgaagg agccggcgcc gtcaattgct tttgctaaaa
      661 actggatttc ggcggcccgc accgtaaaat gccccgcatg aatcgtcacc gtaacagccg
      721 taaaccatgg attatcaccg ctctgctggc tatcgctttg ctgggcacac tgctttcaca
      781 ctgccgtcag gtcgaaaagg attatccgcg cgatgaagcg ccgaagctcg acgaaacctg
      841 gtcgtccggc agcggcaata tcaaagtcgc ccgcattccg ctcaacggcg tcattatgcg
      901 cgctgaagaa gagggatttt tcgccgcacg gtctgacatg gttgaaagca tcctgacaca
      961 gattcgcgca gccgggcgcg atccggacat gcgcgccatt attctggaag tcaactcgcc
     1021 cggcggcggc gtaacaccca gcgacgaaat ttataatgcg ctccgcggct tccgcaacag
     1081 ccggccggac cgccgcgtag tcgtcttcat ccgcgatctc ggcgcatcgg gcgcttacta
     1141 cgccgctatg gccggcgatt tcatcatcgc cgaaccgacc gctgtcgtcg gttccatcgg
     1201 cgtcatcatg cagacaatga atttccaggg gctctccgag aagatcggca tcaccgacgt
     1261 cacgattaaa tcgggcgcca ataaagatat gctcaatccc ttccagccgg tgaacaccaa
     1321 tcaggttgca ctgctgcaga agctgattga tgaaatgcag acgcgcttca gcagtatcgt
     1381 catcgaaggg cgcaaactcg atccggccgc cgcaagcgga ctgttcgacg gacgaatctt
     1441 ttccgccgcc gacgcgctga ataaagggct gatcgaccag atcggctact ggaatgatgc
     1501 gctcgcccgc accgaaaaac tgcttgatgc cggcacactg cgcgttatcc gctacgaacc
     1561 gcagcggaca tttttcgagg aacttctcga aggcaaaagc ccggtcgtac tgccgaatct
     1621 gaaactgcat tcgccgcagt ttttgtattt gtggaaacct taaccaactg aattacggag
     1681 tgatgaaaca atgaaaaaaa cagtctttgc actgctgtcc gccggactgc tgtccggatg
     1741 cgaaaccatg ccctttcaga tacagacacc cgcaacccgc gccgcggtaa cggaggatca
     1801 gcttatcgcg caggaaaatc agcgtaaaat ggccggacgg cttgaaacac ttgaactgca
     1861 aatgaaccag atgagccggg atctcgacac gttgaaagga cagctttcca cccgttgcgc
     1921 ggccattgaa cagaaaagcg aagcggataa acgggaaatg gttgcccggc tgagcggcga
     1981 actggacaag ctgatgaagc tggcttcagc accggttccg gcgccgaaac cgccggccgc
     2041 cacatccggc attgaacatg tcgtccgctc cggtgaaacg ctctacatca tcgccaaggc
     2101 ttacaacgtc agcaccaaga cgattattga cgccaacaga ctgacggatg ccagtaagat
     2161 ttctgtcggg cagaaactgc ttatccccgg tgcgaaataa ccgctggata agctactgct
     2221 ttccgcggcg ccgcaacgta tcgatttccc gctgaagtac tgcagccttc gcttcgtcag
     2281 cggcaagatc gccggagttc ggggcgacag tacgctcccg gacatagccc acatgcatta
     2341 accaggcctt cgaacacagc gtgcggtgct gatcgaccag cgcaaacagc gggtcggcct
     2401 gcgcgctctt cagcaactct tccgccggag catccggcac aggaacctgc agcgcctgca
     2461 gaatcgcctg cgccatgacc aggtgcccga gttccttcgg gtggatattg tctctggtga
     2521 atttaaagtc cggcaccgtt ttctgccgct cggcgcgggc ggccgccatg aaggaatgca
     2581 ggtcgatcac cgccgctaca ccttccggcg gatgctcttt ttcccaggca gcgaactggt
     2641 ccaagaccgt attataaccg ccctcgtaaa cgggcggctt cgcatcggcc gaagaggcag
     2701 aacggtagtt accgccttcg aaaatcggcg gggtcaccag aatcacctgc gcacccatgg
     2761 cgcggcagtc ctgcaccagc cgggtaatcc cgttttgaaa agcccgcatc cgctcttcat
     2821 ccggcggcgc ataaattcca tcgttcatgc cgtaacaggc gatcaccacc tgcggacgaa
     2881 ctttttcgag cgcccgtccg agccgctcat gaacacacgg gcggggaaat tttccgccgg
     2941 catgatttgg ctccgannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaatg
     3061 gtgctcgagc ggggactcga accccgaagg ccttgcggcc accagcacct caagctggcg
     3121 tgtctaccaa tttcaccacc cgagcggaga aaggtcggaa aacataaccg ctcgcttcgc
     3181 cgggcgcaag tgcatattta aattaagctg gccttgaact ccggacgctg aactttcaca
     3241 ctccgcccca tgaaactggt catcgctacg cgcaatcgtc acaagctcga agagattcac
     3301 gccatcttca acttcgcggg gctggaagtc tgttccgcct tcgattttcc ggagattccg
     3361 gatgtggtcg aagacggtgc cacgctggaa gccaatgcga tcaagaaggc atcggaaatc
     3421 tgccgggcca ccggactgcc ggcgctggcc gacgattccg gccttgaagt cgaagcgttg
     3481 aacggcgcac cgggaattta ttccgcccgc tggtcgggcg aaggctgcac ctatcatgac
     3541 aataatgtga agctgctgcg cgaactgtcc gggaaggaaa accggcgcgc gcggttccgg
     3601 acggtgatcg cgctcgccct gcccggcgag acgccgcaaa cggttgaagg ctccgtgcag
     3661 ggttggatta tcgataaaat gcgcggcgat cagggcttcg gctacgatcc gctttttgtt
     3721 ccggacggct acaaccagac cttcgccgaa ctgccggcgg acgtgaagaa ccgcatttcg
     3781 caccgcgccc gcgccctgaa tgctgcttcc aatatctgga agcatttctt ccagggtctg
     3841 gaagcataaa aggcgtcccc gccggagcag ggacgccttt gctgccgggc tgacagaaat
     3901 cagccgatgg ctacatgccc ggtttcgccg gtgcggatgc gcatacactc ttcgaggttc
     3961 aggacgaata tcttgccgtc cccgatctct ccggtccgcg cgccttgtgt gacggcgtcc
     4021 agtgcaggct tcacgaaatc gtcattcacg acgatttcga gcttcacctt tttcagcagg
     4081 ctgcccgctt ctttatggct gcggtagacg gcggcgatac ctttctgccg gccgcgtccc
     4141 atcaccgtag aaaccgttac cagatgaatt tctttttctt cgagcgcatc cagaaccgga
     4201 tccagacggt cgggctgaat aatcgcgact atgtatttca tggttctctt cctttccctt
     4261 agtcaatcgt tgtatagccg gcttctttgt gctgagtcag atccagcccc acccgctctt
     4321 cgtgctcgga aacacgcagc cccataaatt tgtccaccag cttgagcagg acaaacgaga
     4381 cgacgaggga gtaggccatg gtaatggcca ccgctttgag ctgaatcagg aactggcccg
     4441 gattgccgtg cagcagaccg tcaaatccgc cgaccgattt ttcggcccag attccggtgg
     4501 ccagcgcgcc ccagataccg ccgacgccat ggacgccgaa cgcatccagc gtgtcgtcat
     4561 acttgaacac cgctttgatg aacgtgaccg cgatgaagca gatgatcgaa gcccccgcgc
     4621 cgatccagat ggcgctcatc ggcgagacga aaccgcaagc cggcgtaatc gccaccagcc
     4681 ccgcaaccgc cccggtgatc atccccagcg ttgtggcctt cttatgccac agccagtcgc
     4741 agacggccca ggtgagcccg gcgaccgccg ccgcgatatg tgtcgctaca aacgcactgc
     4801 cggccaatcc gttggcgccc agcgcgctgc cggcgttgaa gccgaaccag ccgaaccaaa
     4861 gcatcccggc gcccagcacc gcaaacggaa gattgtgcgg cggcgaaacc gagccgaccc
     4921 gcttgccgag aatcagcgcg gcgaccagcg cagcgacccc ggcgttaata tgcaccacgg
     4981 taccgccggc aaagtccagc gcgccgggca ccgcgttacc ggcggcatca aagcgcgaca
     5041 gaaaaccgcc cagcccccag acccagtggg ccaccgggtc atagaccagc gttgaccaca
     5101 gcagcatgaa gacgcagaac gccgaaaact tcatgcgctc ggcgaacgcg ccgagaatca
     5161 gcgccggcgt gatgatggcg aacatggcct gaaagatcat gaacaccgaa tggggaatcg
     5221 tggcggcgta gccggtaaac ggatcgacgc cgacgccttt cagaaacgcc cagtccagat
     5281 tcccgatcca gcctttcaca tccggcccga acgccagact gtatccgcac agcgcccact
     5341 gaagggtgat gagacagatc accgcgaaac attgcatcag gatcgacagc acattctttt
     5401 tacggacgag tccgccgtaa aagaaggcca aacccggaat catcaggaaa accagcgcgg
     5461 cgctgataag catccacgcc gtatcgcccg cattaatcgc cggtgccggc gccggcgtgc
     5521 tgagcgctcc ggcgaccaca tcctgaccaa acccatttcc cgcaagcatc accaagatgc
     5581 tcaaccaacc acagagcaga ccggctctgc caaatacatt ccttttcatg cagcatacct
     5641 cctgtttgca ttccggtatg cttaaagcaa cgactgtgcc tgtattttgg atgaccggtc
     5701 taaaatatat gcaacattcg ttttattaac gaattaaaaa aatataacga cctgttacat
     5761 ctgcggcgct aaatataaaa cgaacgccat ttttgtaatt tacagcatct atgaagcaaa
     5821 gttgtagccg ttgatgatcg catcaacctc gctgatgcgg ctggcggaca gcatccggcg
     5881 agccgtatag accgccttct ccatgctgat cttttcaatg acctgctgaa tctcatgaac
     5941 ccggcccggc ggcagactca acttcgtaat ccccgcaccg agcaggaacg ggatcatttt
     6001 cggatccatg gcgatatcgc cgcaaatgga gacatccttg cgatgactgc gcgctacgga
     6061 agccacccgt tcaatggcgc gcaaaatcgc cggatggtgc gacgtgtaga gtttggaaac
     6121 catctcgttc gtgcggtcaa ccgccagcat gtactgaatc agatcgttcg ttccaatgga
     6181 cataaagtcc acttcatggg cgaggtcttc aatcagcgcc acggcggacg gcagctccac
     6241 cataatcccg atcttcgtgt tccggttgta cggcacaccg cgcgcgtcga gctccttcat
     6301 gcagtcatac accaccgcgc gtgcctcgat gaaatcgtcg agcgaggcaa tcagcgggaa
     6361 catgatgcgg gtattgccgc cggctcccgc gcggagaaac gcccgcagct gctcggcaaa
     6421 aagatctttg tgcagcagcg aaaaccggat ggcgcgcagg ccgagaaacg ggttggcctc
     6481 ttccgcttcg ttctgatagg tgagcacctt gtcgccgccg atatccagcg tgcggaacgt
     6541 cacctcgcgc ccgcccatcg actcaacgat ccggttataa acctgatact gctcttcttc
     6601 ggaggggaag gcgttgcgga tgatgaaggg gaattcgctg cggtaaagac ccacgccctc
     6661 ggtatgaaaa ctcagcgcca gcttgagatc gctgagcaga ttgatgttgg caaacagctg
     6721 aacccgggtg ccgtcggcgg tgcgcgtttc ggccttcacc ggcggagcgg tctccagcgc
     6781 cttggccgcg cggcgatact cgttatattc cttcagaacc tcttcggacg gctgaatgaa
     6841 aatcgacccc tggttgccgt cgatcagcag ttcgcacggc ttgccgagca gctccagctg
     6901 ctgcggatca atcatcacca tcggcttctg gagcgagcgg cagatgatcg cgttatgcga
     6961 agtcacgccg ccctgaacaa caaagccttc cgcattttcc gtcgccagct tgaggatatc
     7021 ggacggcagc agcgcatcgg cgacaacaat ctgcccgctg taatcgccgc gctcgtgctc
     7081 gccgccgatc agattttcca gcagacgatg gcccagatcc ttcaggtcgt gaaccttctc
     7141 ctgcaggcgc agattggggc tggccgaaaa gatttcaata taactgttca ccagcgccac
     7201 cagcgcttcg gccggcggcg tcccggccac aatccgccgg cgcatctcgc cggaaaattc
     7261 ctcgtccgcc agaatcagca gatgcgcatt gaaaatcagc tgggcgacat cggaaagctc
     7321 ctcgtcggcc cggcgctgga gatctttgag ctgctgttcg gtcagctgca gggcgcggtc
     7381 aaaatcctgt acggtcagat tctgcggcgc gccctccggc acctggaaac tgccgccgcg
     7441 gcctgaaata atgatcgccg tccgcccctg cgccacaccg ccgctggccg gacgcccctt
     7501 gatcatcttg gggagcgtac ggacctcatc cggcttagca aaatttttct gccggtgcag
     7561 cgtcagcagc agattggcgt tttcaatcac cgtcgccagc tgcgcggcaa tcgccttcag
     7621 cgcctgttca tctttttcat caaaatagtc cggctgaacg tcctgtaccg tcagcacccc
     7681 gacgcgcgtc agtccgcgcc ggatcggcac ggccaggaac gcctgatagc gctcctcatt
     7741 gatgccgggg accggctgaa acttcggact gagaacgccg cggccttcac ggatggcgcg
     7801 cagctctttc agtgcaagcc cagtcagccc ctgtcccagc cgcagcgtca cccggccgat
     7861 tgcatccgga ctcagcccct gcgtggcgcg cagcgtaagc atccgctcct tgtcgtcata
     7921 gatataaatc gagcagaccg ccgccttcat atgccaggca accacgctca cggcggactg
     7981 cagaaagtcg tcgagactgc tgcttttctc aaagagcgac gacagttccg caatgtcgca
     8041 gatcatattg acgttcgctt tacccataaa agccccctgt aaaaacgaac cagttctact
     8101 ctgcttcggc aaagtttgga agcgctaagg ttttgttcaa cggtgtttcg tgtgcgggcg
     8161 gagctgcttc atctgggtgc ggcgctcggc gaggcctcgg atgaccggaa cgggctcgcc
     8221 ggtttcgtag tcgaggccgg tgtaataaac ggcggcggcg ggcgtcatcg gcagcggaat
     8281 aaaatcctgc acctgctgga gcttccactc ctcttcgcgg acaaattttt caaccgcctt
     8341 catttccgct tcggtcgagc cgggaaagtt ggaaatgaag tacggcacca gatactgctt
     8401 tttgccggcg gcacggcttt cggcctcaaa ggctttctga aactcgcaga aggtttccgg
     8461 tcccggtttg cgcattttct tcagtacgtt cggatgcatg tgctccggcg ccactttcag
     8521 atggccggaa acatggtgct tcgccagctc gcggatatag tgcggattgc gcagcgccac
     8581 ttccatgcgg atgccggact gaataaagac atgcttcacc ctggccagct cgcggacgcg
     8641 cttcatcaga tgaatcgccg acccggcgtc cagttcgagg ttcgggcaga ttttcggata
     8701 gaggcagctg gcgcggcggc agactttgca cgccgggcag acgccgttga cgcaaccgta
     8761 gagattggcg gtcggcccgc cgagatcggt gatggttccg gaaaacgacg gcatcgccgc
     8821 cagggttttc acctcgcgca ccaccgactc ctccgaacgg ctcgtcagaa accggccctg
     8881 atgcaggccg agtccgcaga aggcgcaccc gccgccgcag ccgcgcacca ccgtcaccga
     8941 ctccttgatc atcgtaaagg ccggaatcgg ctctttatag gaaggatgcg gcagccgcat
     9001 atagggcagc tcatacaccc ggtccatctc cgccgccgac agcggcgcgg ccggacgttc
     9061 gatcagcacc gcccgcgcgc cgtgaaactg caccaacggc ttgccgctga acggattctg
     9121 ctcgcgctcc gtcagccgcg tcagctccag caccgcttgt ttatcggcct ggcacgcctc
     9181 aaaggacggc agccggacgc agtcggcaaa atcgcgctcc gccgactctt taccgccgag
     9241 cagccgggcc gttccgggga ttccgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnntcgcg gatggcccgc tcgcccatgc cgtagaccag cagatcggcc ttggaatcct
     9421 gcaaaatgga ggggcgcagc ttgtcctgcc agtaatcgta gtgcgccgtg cgccgcaggc
     9481 tggcttcgat gccgccgagg acggtcgtca tgccgggaaa cgcctttttc gccagctgcg
     9541 tatagaccat tccggccaga ttggggcgct tgccggccac gccgcccggc gtgtaggagt
     9601 cttctttgcg tttgcgccgc gcggcggtgt agtgcgacag catcgaatcg aggttgccgg
     9661 ccgtgatgcc gcagaacagg cgcgggcggc ccagcaccgt cagcgattcc ggcttcgtcc
     9721 agtccggctg ggcgataatg ccgacccgcc agccgtcggc ttcgagcacg cggccgatca
     9781 ccgccgcgcc gaacgaggga tggtcgacgt aggcgtcgcc ggtgacgatc aggacatcca
     9841 gttcgtccca gccgcgcgcc ttcatttccg cgcgtgtcat cggtaaaaat tgtttcgctt
     9901 ccgccatggg ggaagacggt ctcaaatttt ccaatccttg gaaattttat tcgacctgac
     9961 tggacgggca acctactatg ccgccctgat ttaactctaa acgacatgca aaaattagac
    10021 acccaactct ttctcggcct ctaccgtcac atgctggcgt cgcgtaaagc cgacgctgtc
    10081 caggaagacg ccgcccagcg cggcgaggct tttttctata tcccctcgtc cggtcacgag
    10141 gcgatggccg cgcttgcgcc ccatctgacg ccgaccgact ggctgcactg ccactaccgc
    10201 gaccgcgcgc tgatgctcgc ccgcggcgtc acccagcagg atctgctgct cgacctgctc
    10261 ggtaaaaccg gctcgccgac ggaaggccgc aatatgcccg gcttcgcctg caaccgggcg
    10321 ctgcatctgt tcggggcgcc gaccggcgtc ggcagcaacg cgctgcaagc cgtcggcgcg
    10381 gcacaggcga tcaaggacag ccgcgacctc gtttactgcg gcatcggcga cggcggtacg
    10441 caggaaggtg agttttttga agcggtggcg gaagccgtcc gctcgcagct gccggttctt
    10501 ttcgtcgtcc agaacaaccg ctatgccctt tccacgccgt ccaaaggccg cacctttttc
    10561 tcgctgccgg agggcgacgc caaggagttc tacggcctgc cgattctgcg cgccaacggc
    10621 accgatgccg tcgaaaccta ccgcgtgttc ggcgaagcgg ttgccaaaat ccgccaaacc
    10681 cgcggcccgc agctcgtcgt cgtcgatatg gagcggctga ccagccacac caacgccgac
    10741 gaccatacgc tttaccgctc ggccgacgac atccgcgaaa tgcacgaaaa ggccgacccg
    10801 attctgattc tggccgaaaa gctgatcgcc gccgggattc cggaagccca gctcaaagcc
    10861 atcgagcatg aaaccaacca gcagctgacc gccgcgtttg acgccgcccg caaagcgaaa
    10921 aatgccaaga ccgagctcag cgccaaaaag ccgctacccg cccggctgac ggatgccgcg
    10981 agcgaaaagc gcacggccgg cgacgcgctg acgatgctcg aagcgatgcg cgccgtactg
    11041 cgcgcccggc tcggcgaaga taaaaccatc tatctctacg gcgaagacct cgaagatccc
    11101 aaaggcgatg tcttcggtct gacccgcgga ctgagcaccg cctttcccgg acagatcatc
    11161 aacgccccgc tcagcgaggc caccatcgtc ggcgcagcca tcggccaggc gatggtcggc
    11221 aaaaagccgg tcgcctgtat tcagttcgcc gatttcatgc tgcccgcgtt caaccagatc
    11281 gccgccgaac tcggcgcgat gtggtggcgc acgaacggac agtgggaatg ccccgtcatc
    11341 ctgatggcga tttgcggcgc ctaccgcccc ggcctcggcc cgtatcacgc gcagaccttc
    11401 gacgccactt tcgcgcatat tccgggcctc gacgtcttta tgccctccac ggcgccggat
    11461 gccgccggcc tgctcaacgc cgcgttcgaa tccggccgtc cgaccgtgtt cctcttcccg
    11521 aaaaatctga ttaacgaccg ttccgtcacg gcggccgcag atgcggcgac gcagttcgta
    11581 ccggtcggta aagcgcgcgt cacccgtccg ggccgcgacc tgaccatcgt cagctggggc
    11641 agcacggtgc cgctgtgcga aaaaaccgcc gacgcgctgc agaacgccgg ggcgtccgtg
    11701 gaagtgatcg acctgcgctc gctcgttccg tgggaccagg aaaccgtcat cgcgtccgct
    11761 cagaaaaccg gaaagctgct cgtcgtccac gaagacaacc atacctgcgg attcggcggc
    11821 gaagtgatgg cgacggtcgc cgaaaaagcg aacgtgccgg tgcagatggc gcgggttgcg
    11881 cggcacgaca cctatattcc ctatttgttt gagacgcaaa tcgaagtgct gccgtcgttc
    11941 cgcaccgtac tcggcaaagc ggccgagctg ctcgactatt cgctgacctg gcagaagccg
    12001 gtggaggaag ccgaaggctc cgtcaccgtc aacgccatcg gctccagccc gtcggataaa
    12061 acggtgaagg tggtcgaact gcacgtgaag gccggacagc cggttcaagc cggtgaactg
    12121 ctcgcgtccg tcgaagccga taaggcgacg atggaaatct cctcgccggt cggcggcatc
    12181 gttgaagcgc tgttcctggc cgaaggcgat tccgtcgacg tcggcacgcc gctggcgcgc
    12241 atcaccacgg aagagatcag tctgattaaa aagccggtga ccaaagacga tcccggcacg
    12301 ccggttctgg aaaagaaact ttccgccgcg aaagcgaaaa ttaccgcggt tgaacacaag
    12361 ccggtattga tttcgtcgat cgccacggtg ctcggctcgc gccgcatcct gaacgaagag
    12421 ctgctgaaag gccacggcga gtgggattcc gaagcgatcc gctcgcgcac cggctgcgaa
    12481 gagcgctact ggatcaccgg cgacgaaaac gtcgaaacgc tggccgtgaa agccgtgcgc
    12541 gacctgctgg agatggaaaa actgacgctg gccgacatcg atgccatcgt ctgttccacc
    12601 ggtacgccgc tgatgatgac cccgtcgctc gcctgccgcg tgctcaaaga actcagcccg
    12661 gaaaagggcg aggttcagat gcaggcccac gatgtcagcg cggcctgcgc cggctatatg
    12721 tatgcgttgc agaatgcggt ggatatcctg cgcgacgatc cgtcgaagaa agtgatcatc
    12781 atcaccgccg aaaccctttc gccgatgatc aaccatgagg accagaagac gctggcgctc
    12841 ttcggagacg cggccacggc gtcgctgctt tcctgcgaac cgcgcggcgg caatgtcaac
    12901 gttctggtga accgccccgt tctgtcggcc gtcggggtcg accagaaggt gctgtatgtc
    12961 ccgcacatgg gcaccggcga gaagatcgaa atggaagggc tcacggtctt caagatcgcg
    13021 gtgcgcaaga tgatcgatat gctggcggaa gcctgcgaaa tgcgcggcgt cgcggtggat
    13081 gcgctcgaca agatcgtgcc gcatcaggcc aacgagcgga ttatcgaagc gatccgtaa
//
LOCUS       DAOPZO010000042        13048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_5278_length_13048_cov_9.035404, whole genome shotgun sequence.
ACCESSION   DAOPZO010000042 DAOPZO010000000
VERSION     DAOPZO010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 13048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13048
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5278_length_13048_cov_9.035404"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1645
                     /locus_tag="PKI68_04225"
     CDS             <1..1645
                     /locus_tag="PKI68_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HNX52929.1"
                     /translation="DALFRVPVQLASSCPHGRCAFCSENGAKAQQTQRQESPVEQIEA
                     AIRFSKRRYKAQKLMLYIQAFTADLTDPVQQELILKCLNDYHFEAISIGTRPDCLPEA
                     ALRFLESLNEKTEVWVELGVQTATDETLRRINRGHDWECSRQAIRALAARGVHVAPHI
                     IIGLPGEAQADWNRTAAELAKLPIAGIKIHNLHVVKGTRLAENPPPVLSHWEYAEALM
                     EFLRRIPAGIPVMRISTDTPDDELLAPHWHLEKGQFLDYVTQQMTMREIRQGDLTEIG
                     NLKPEIGMEPIQTEDGSITFFSNDWKEHYHTKTGARLEALKKFVEPSKLAAKLQTGNV
                     SLLDVCFGLGNNSLAALSAAVNAEHRLAITALEMDKRVVRAAAEHFHPLESDPAEWKK
                     ILLELLDSNQSALGNHQLAIQWGDARWLIQNLESGAFDLIFHDPFSSQHCPELWTVEF
                     FQQLYRVLKPDGVLLTYASALPVRGAMRAAGFAVGATHPGQPMGDGTIAAKRAKDIEC
                     PVPEAPDERRNIPYRDPHLCATAKTILRNRQEAVEITNR"
     gene            1713..1988
                     /locus_tag="PKI68_04230"
     CDS             1713..1988
                     /locus_tag="PKI68_04230"
                     /inference="COORDINATES: protein motif:HMM:NF019214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HNX52930.1"
                     /translation="MDVWMILGFFAQAVFSLRFVVQWIASERAGESVVPELFWLISLT
                     GSLLLLLYAIHQRDPVFILGQGSGIFIYLRNMHLIIRKKKCAQAIGN"
     gene            2178..3806
                     /locus_tag="PKI68_04235"
     CDS             2178..3806
                     /locus_tag="PKI68_04235"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNX52931.1"
                     /translation="MNVSFRKYSFLLLAGLWLVTLIGIQSVPLETHEVYVLQTAQEMQ
                     TSGDWILPHFNDSLRLQKPPLSYWATLLISKLDPLNDNIQIWHGRLVSMLAGLLMVLA
                     TARAGTRFYGGCKTGLLAALLLLCTQGFINLSHSARPDFLYSTFCFLQLVAWMEAWMA
                     EDGTPRQRWSSLLGWAAAALAVLTKGPQVPAVFLLGLLIFLLSRTERKRTLRILRPVS
                     GTALLCVIVLPWWILLNRRLQSLGVNIEDSQLSGSLLRNLAGWKELFSGYYLWTLFVL
                     LLPVSLAVPFIIRKLWRGRKQIGAASRLLITVSAALLLVFTLGGHYRKHYLIPLLPVL
                     ALLLARAVYLLPATGLPARWKKPLAAVGALAGLGAAGLLFYRGAYVMLLVLPAYTLLL
                     FFVWKKVLAHTHWDTQRLATRLPAYAIFTTVLLSVYVGFLPMNPNRVDEQVFSEFIAR
                     TLTPDDLIITWKCSPANLPYYARRTVTCYTDSETLKSCLAGNRTDRPVFAVLPAGELE
                     AFSAAFPNDVLKETANYRKKDEHYLFIRLRSAGN"
     gene            complement(3807..4415)
                     /locus_tag="PKI68_04240"
     CDS             complement(3807..4415)
                     /locus_tag="PKI68_04240"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNX52932.1"
                     /translation="MRVRVYSAALLMLVLMFVCYRYVDIPVMEYFGKNKIFDSAGDFI
                     SMIGRYYDVVIGLILAGWFWYHRRSAAQRRKCFFFGACWGLSNAAGAILKVLCGRYRP
                     KMLTEHDLYGFHGHGTTHGLASFPSGHTLDAVAIAAALWVLWPRSRPWCVAWAVSMGL
                     ARIVVRAHYVSDVLAGVLIGCLTVYLINEWLKPAEFKGDSQP"
     gene            complement(4412..6190)
                     /locus_tag="PKI68_04245"
     CDS             complement(4412..6190)
                     /locus_tag="PKI68_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNX52933.1"
                     /translation="MNLNKIDQKAGCDKPDSPVWLRYAALLFLCLTLYLPGMTTIPPI
                     DRDEPRYAQASRQMTETGDYVQIRFMEQARNKKPIGIYWLQAGLANLTGHPEVIWPYR
                     LASVLGSTLAVLALFSFARKFLPERTAFFGAAFLAACPLMNFVSHAAITDTVLLSTVV
                     TAQGCLALIYFAARSGKAVPLSCVAGFWLAQSAGILVKGPIAPMVSLLTILTVSACEK
                     NIRWTRSLRILPGLLVVFLCILPWLIAVQTATDGAFLRQALGKDFLPKLYAGQESHGG
                     LPGLYLLMTPVMLWPIFLPVCQGLFKAKNRVADPRILRFLFAWLIPAWLVFEFAATKL
                     PHYVLPAYPVLAVLAAIMATRPADEPVQVRSVWFRALTGIWVAVGMVLAFAPLLFSHL
                     LDGRWSPAAFGLMAIMLAGLLTLPKLLKQRSRFYPWSVLVFSVALSVTAFGLWLPQLN
                     AFWPTRQAADMVHRYEQQAGHPVRTLSLGYAEPSLAFMLGTKTGIEWDLDRILNELKT
                     NPDAVVIVQAAADPIPKRFPVSDKLWQALDKGLTLKAKNCYEERFLAAAAAAGLKLRK
                     QDVTDGYNYSKGKRVKLTLYVPEPSV"
     gene            6314..7900
                     /locus_tag="PKI68_04250"
     CDS             6314..7900
                     /locus_tag="PKI68_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNX52934.1"
                     /translation="MRSALNDLKIPLLSLLLTGLMAAFFLPPMPIDETRYLSVAWEMQ
                     HAGSYVVPLLNGEAYSHKPPFLFWMLQAGWKLFGVNDVTPRLTICLFGMLCILLLRKI
                     SLRLWPEEKKTAACAALVLGSTLIWMLWSCAIMFDVMITVWVLISILGILSAETAPKK
                     GWILLTLGIAGGMLTKGPAIMVYTLPLVLLRPLWAADRAVRWNLLTAFLAGTGIALLW
                     AVPAAILGGEEYRNAIFWGQTVNRIGSSFAHRRPLWWYVPILPLLFLPWIFFRPALAK
                     IDFKKADRGIRLAVLWLAVPFVIFSLISGKQVHYLVPIIPAGALLMGRNIARSENLTG
                     TACVKVIGGFYCLMGIVALVLPFIHFSEKIGQLDQGSTWIAAGGLFCAGVLLMRPFRS
                     TGSAAKALALSTVIILLFGLVEANRSFEGYDIKGIAVAVKGKMDEGCPVAHIGKYYAQ
                     YQFLGRLPRPLTLVSSKTDTDAINQFIADHPNGVLISYLKKKDQLPENAVVYYRQRYR
                     GETSVVLWGPPRQPAAETAP"
     gene            7897..8568
                     /locus_tag="PKI68_04255"
     CDS             7897..8568
                     /locus_tag="PKI68_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX52935.1"
                     /translation="MKILLIEDDSRTAAFIIKGLQQEGFAVDRAADGEEGLHLALQNS
                     FDVAVVDIMLPKIDGLTLIERLRKQKFTTPIIVLSARSSVEDRIKGLHVGADDYLMKP
                     FAFSELLARIQALLRRAGAQSADPTALTVEDLRLDLVRRKVYRAGKEIELQPKEFSLL
                     EYLMRNSGRVISKTMIMEHVWDYNFDPQTNVVEARICKLRDKIDRDAEKKLIHTIRGM
                     GYVLE"
     gene            8555..>9678
                     /locus_tag="PKI68_04260"
     CDS             8555..>9678
                     /locus_tag="PKI68_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNX52936.1"
                     /translation="MCSNKPRRPARSVVARLTFGYAVICTLFCLTVFSVVSAKMSANA
                     RKRIDRSLTAELAELSTIYRQTGLKSLQDEFDQEAGLNGANKLFYRLLSPEQKPVAGS
                     NMSGWKNLEEELRTIPAPTNNGPVFSTIYARNSPFNARMATVKTADGSLLQLGINLRS
                     DNHFQEKINRVLIVSSLILIVLSTLSGWLIARRAMSGVQRVTQAVASIRKGRLNQQVP
                     LGNEGREIDELAEAFNKMLSRIDLLVRELKEVSDNVAHDLRSPITRMRGAAETTLTGP
                     QELEAYREMGVTIIEESERLTQMINTALEIAQAESGILEINHAPLDLTAVLKNAVDLF
                     QPVAESRSLNLRADIPDDPLIIYGDKMRIQRTVANLLDNA"
     assembly_gap    9679..9778
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9779..11556)
                     /gene="aspS"
                     /locus_tag="PKI68_04265"
     CDS             complement(<9779..11556)
                     /gene="aspS"
                     /locus_tag="PKI68_04265"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNX52937.1"
                     /translation="MHKFRTHTCGQLRKENVGQTVRLSGWVNTIRDHGGVLFIDLRDH
                     YGITQIVISPDKAFYKNIEHWRVESVLCFTGSVVARDTAAVNPKLITGEIELVAETME
                     MLGESEVIPFQVNKEEECSEALRLKYRFLDLRRKSLHDNIVLRSKVISRIRQLMTEQG
                     FLEYQTPILTSSSPEGARDYLVPSRVHPGKFYALPQAPQQFKQLLMVSGFDRYFQIAP
                     CFRDEDARADRSPGEFYQLDMEMSFATQDDVFAVNEKLLSTIFKEFSTKKVDGIPFLR
                     IPYREAMEKYGSDKPDLRNPLIMQDASAIFRNCGFKAFAGVVESGGVVKTIAVKGCAG
                     QPRKFFDDMVAFAQSVGAKGLGYISWVDGEVKSPIAKFLSADEIAQLKALGGVGDGDT
                     MFFIADTAKNAQSIGGAVRTELGNRLDLLEKDVFRFCWIVDFPMFELDDEGKVAFSHN
                     PFSMPQGGMEALLTKDPLDVLAFQYDIVCNGTELSSGAVRNHSPELMIKAFDIAGYGR
                     EVVETKFPALHRAFKYGAPPHAGIAPGVDRMVMLLADEPNIREVIAFPMNQKAEDLLM
                     GAPGEVELKQLRELHIKLNLPKKQEK"
     gene            complement(11560..12819)
                     /gene="hisS"
                     /locus_tag="PKI68_04270"
     CDS             complement(11560..12819)
                     /gene="hisS"
                     /locus_tag="PKI68_04270"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNX52938.1"
                     /translation="MASTSYQPIQGMSDIAAPDVALWQMIEQRAREIFARYRFSEVRT
                     PVLEKLELFTHSLGDTTDVVTKEMYTLEDRGGRKFALRPEGTAGAIRYVASGGEETAN
                     ARIFYMGPMFRCERPQAGRKRQFHQIGVEAVGAPNPLADAEVIALQLHLLKAWGLEGA
                     KIRLNTIGLPEDRAAVLEGLREAIRPRLSELPEEAQQRFETNVLRLLDSKDEAVKKVI
                     ADVPPVLEFMSAGSRAYLNTVVETLRKLEIDVSIDSSLVRGLDYYVHTVWEIVHSGLG
                     AQNALAGGGRYRIDVGGRSIDGVGFAMGVERVIASLEATGITAAQFAPPPPVFIVSLG
                     EAALNENLLLLQTLRQRGIACEMELAPRKVQAQMRRADKAGAQMVIIRGDTELEKGTF
                     VLKKMADGSQSEVELPELMNLLTETGI"
     gene            complement(12822..>13048)
                     /locus_tag="PKI68_04275"
     CDS             complement(12822..>13048)
                     /locus_tag="PKI68_04275"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNX52939.1"
                     /translation="DVAAILQKSLDYIVEALNKGETVEFRNFGVFEINVRKARIGRNP
                     NKPEDVVTIPERRVVKFKPGKIMKQQITGI"
BASE COUNT         2710 a         3893 c         3678 g         2667 t
ORIGIN      
        1 cgacgcgctg ttccgcgttc ccgtccagct cgccagctcc tgtccgcacg gccgctgcgc
       61 gttctgttcg gagaacgggg ccaaggcgca gcagacgcag cggcaggaaa gtccggttga
      121 gcagattgag gcggcgattc gattttccaa gcggcgctac aaggcgcaaa agctgatgct
      181 ctacatccag gcgttcaccg ccgatctgac cgatccggtt cagcaggaac tgattctcaa
      241 gtgtttaaac gattaccatt tcgaagccat cagcatcggc acacggcccg actgcctgcc
      301 ggaagcggcg ctccggtttc tggagtcgct gaacgaaaaa accgaagtct gggtcgagct
      361 gggcgtacag acggcgactg acgaaacgtt gcggcgcatc aaccgcggcc acgactggga
      421 atgcagtcgg caagcgattc gggcgctggc ggcgcgcggg gttcacgtcg cgccgcatat
      481 catcatcggc ctgccgggcg aagcgcaagc cgactggaac cgcaccgcgg cggagctggc
      541 caagcttccg attgcgggaa tcaagattca caatctgcat gtcgtgaaag gaacccggct
      601 ggccgaaaat ccgccgccgg tgctcagtca ttgggaatac gccgaggcgc tgatggagtt
      661 tctgcgccgg attccggcgg ggattccggt gatgcggatt tcgacggata cgccggacga
      721 tgaactgctc gctccgcact ggcatctgga gaaaggccag ttcctcgatt acgtcacaca
      781 acaaatgaca atgcgtgaaa tccgccaggg cgatttgacg gaaattggaa acttgaaacc
      841 tgaaatcgga atggaaccaa tccagactga agacggaagc atcacctttt tttcaaatga
      901 ttggaaagag cactaccaca cgaaaaccgg cgcgcggctg gaggcgctga agaagtttgt
      961 cgagccgtca aagctggcag ccaaacttca aaccggaaac gtcagcctgc tagatgtctg
     1021 tttcgggctc ggcaataact cgctggcggc actgagtgct gcggtaaatg cagagcatcg
     1081 gctggcgatt acggcgctgg aaatggataa gcgcgtggtc cgcgccgccg cggagcattt
     1141 tcatccgctg gagtccgatc cggcggaatg gaaaaaaatt ctgctggaac ttttggattc
     1201 gaaccaatcg gcactcggca atcatcagct ggcaattcag tggggcgacg cgcgctggct
     1261 gattcagaat ctggaatccg gcgcgttcga tctgatcttt cacgatccgt tttcgagtca
     1321 gcactgtccg gagctgtgga cggtggagtt tttccagcag ctctatcgcg tgctgaagcc
     1381 ggacggtgtt ttgctgacct acgcgtcggc cctgcccgtg cgcggcgcga tgcgcgccgc
     1441 cgggtttgcg gtcggcgcaa cgcatccggg ccaaccgatg ggcgacggca cgatcgccgc
     1501 gaaacgcgcg aaggatattg agtgtcccgt tccggaagcg ccggatgagc gccggaacat
     1561 tccgtaccgc gatccgcatc tctgcgcgac cgcaaagacg atcctgcgca accggcagga
     1621 agcggttgag attaccaatc ggtaatcttc gctgaatcct gcggcaatct ccgttccggc
     1681 agtatcccgg aatcagttca aggaggatgc ccatggatgt ctggatgatt ttaggttttt
     1741 ttgcgcaggc ggttttttcg ctccggtttg ttgtgcagtg gattgccagt gaacgggccg
     1801 gcgaaagcgt ggttccggaa ctcttctggc tgatcagcct gaccggttcg ctgcttctgc
     1861 tgctgtacgc catccatcag cgcgacccgg ttttcattct gggccaaggc agcggaattt
     1921 tcatttacct gcgcaatatg catctgatca ttcgaaagaa aaaatgcgcc caggcgattg
     1981 ggaactgacg ctttcgatca ttgcgtcata aatccaaccg ccgacttcac cggctgcatg
     2041 ccggcacgag cggttcttca cggttcctgg cttccctgtc ggcctctttt gtcgtataga
     2101 cgactatgat ggaacgccgc ttggtgcggg aagaagccgc aaagtgcgtc tttcaaaatt
     2161 ttaaaccgga atcttttatg aacgtctcat tccgcaaata cagcttcctg ctgctggccg
     2221 ggctctggct ggtgacgctg atcggcattc aatccgttcc gctggaaacg catgaggttt
     2281 atgttctgca aaccgcccag gagatgcaga cctccggcga ctggattctg ccgcatttca
     2341 acgactcgct ccgcctgcag aaaccgccgc tcagttactg ggccacgctg ctgatttcaa
     2401 agctcgatcc gttgaatgac aatatccaga tctggcacgg acggctggtt tccatgctgg
     2461 ccggcctgct gatggtgctg gcgacggccc gcgcgggaac gcggttttac ggcggatgca
     2521 agaccggtct gctggccgct ctgctgctgc tgtgcacgca gggcttcatt aatctgtcgc
     2581 acagcgcccg tccggacttt ctctattcaa cattctgttt tctgcagctc gtggcctgga
     2641 tggaggcctg gatggccgag gacggaacgc ctcgacagcg ctggagcagc ctgctgggat
     2701 gggctgccgc cgcgctggcc gttctcacca aaggcccgca ggttccggcg gtctttctgc
     2761 tgggcctgct gattttcctg ctcagccgca ccgagcgtaa acggacgctg cggattctgc
     2821 gcccggtcag cggaacggcg cttctgtgcg tgatcgttct gccgtggtgg attctgctga
     2881 accggcggct gcaatcgctc ggcgtaaata ttgaagacag ccagctctcc ggatctctgt
     2941 tgcgcaacct ggccggctgg aaggaactgt tcagcgggta ttatctctgg acgctgtttg
     3001 ttctgctgct gccggtcagc ctggcggttc ccttcataat ccggaaactc tggagaggcc
     3061 gcaaacaaat cggcgcagcc tcccgcctgc tgataacggt cagcgccgcg ctgctgctgg
     3121 tgttcacgct gggcggccac taccgcaaac actacctcat tcccctgctg ccggttttgg
     3181 ccctgctgct ggcccgggcg gtttatctgc ttccggccac cggccttccg gcccgctgga
     3241 aaaaaccgct ggcggccgtc ggcgcgctgg ccggactggg tgctgccgga ctgctttttt
     3301 accgcggcgc gtatgtgatg ctgttggttc tgcccgctta caccctgctg ctgtttttcg
     3361 tgtggaagaa agtgctcgcg cacacccact gggacacgca acggctggcg acccggcttc
     3421 cggcgtacgc catttttacg accgtgctgc tgtcggtata tgtcgggttt cttccgatga
     3481 atccgaaccg ggtggacgaa caggtttttt cggaattcat tgcacgcacc cttacgccgg
     3541 atgacctgat tatcacctgg aagtgctcgc cggccaacct gccctactac gcccgccgga
     3601 cggtcacgtg ttacaccgac agcgaaacac tcaaaagctg cctggcagga aaccggacgg
     3661 accggcctgt gtttgcggta ctgcctgccg gtgaactgga agcgttcagc gccgcctttc
     3721 ccaacgatgt gctgaaggag accgccaact accgcaaaaa ggacgaacac tacctcttca
     3781 tccgactgcg cagcgccgga aactgatcag ggttgcgagt cgcctttgaa ttcggccggc
     3841 ttgagccact cgttgatcaa gtagacggtc agacagccga tcagcacacc ggccagcacg
     3901 tcgctgacgt agtgggcgcg gacgacgatg cgggccagcc ccatgctgac cgcccaggcc
     3961 acacaccacg ggcggctgcg cggccataaa acccacagcg ccgccgcgat ggctacggca
     4021 tccagcgtat ggccggacgg gaacgaggcc agcccgtgcg ttgtgccgtg cccgtgaaat
     4081 ccgtaaagat catgttccgt cagcatcttc ggacggtacc gtccgcacag aactttcaag
     4141 atcgcgccgg cggcgttgct caggccccag caggcgccga aaaaaaagca ctttcgccgc
     4201 tgcgcggcac tacgccgatg ataccagaac cagccggcga ggatgagacc gatgaccacg
     4261 tcatagtagc ggccgatcat cgaaataaag tcgccggcgg aatcgaagat tttgtttttg
     4321 ccgaagtatt ccatgaccgg aatatcgaca taccggtaac agacgaacat cagcacgagc
     4381 atcaggagcg ccgcactata aacgcgaact ctcatacgct gggctccgga acatagagtg
     4441 tgagtttgac gcgcttacct ttggagtaat tgtacccgtc ggtgacgtcc tgtttgcgca
     4501 gcttaaggcc ggccgcggcc gccgccgcca gaaaccgctc ctcataacag ttcttggctt
     4561 tcagggtcag ccctttatcc aatgcctgcc agagtttgtc ggaaaccgga aaacgcttcg
     4621 ggatgggatc cgccgccgcc tgcacaatca ccacggcatc cggattggtt ttcagttcat
     4681 tcagaatccg gtcgaggtcc cattcgatgc cggttttggt gcccagcata aacgccagac
     4741 tcggctcggc atagcccaga ctcaacgtac gcaccggatg cccggcctgc tgctcatagc
     4801 gatgcaccat gtccgccgcc tggcgggtcg gccagaacgc attgagctgc ggcagccaga
     4861 ggccgaaggc ggtcaccgaa agcgcgacgg aaaaaaccag aacactccag ggataaaacc
     4921 gggagcgctg cttcagcagc ttcggcaacg tcaaaagacc cgccagcatg atcgccatca
     4981 aaccgaaggc ggcaggactc cagcggccgt ccagcagatg gctgaaaagc agcggcgcga
     5041 aggccagaac cataccgacg gcaacccaga tcccggtcag cgcccggaac cacacgctgc
     5101 gaacctgtac cggttcgtcc gccggccggg tcgccatgat ggccgccagc accgccagca
     5161 ccggataggc cggcagtaca tagtgaggca gtttggtggc cgcaaattca aagaccagcc
     5221 aggccgggat gagccacgca aataaaaacc ggaggatgcg cggatcggcg acccggtttt
     5281 tcgctttgaa gagcccttgg cagaccggaa ggaaaatcgg ccacagcatg accggcgtca
     5341 tcagcagata gaggcccggc aggccgccgt ggctttcctg tccggcgtag agcttcggca
     5401 gaaaatcttt gccgagcgcc tgccgcagaa aggcgccgtc ggtcgcagtt tgcaccgcaa
     5461 tcagccaggg aaggatgcag agaaagacca ccagcagtcc cggcaggatg cgcaggctgc
     5521 gggtccagcg gatatttttc tcacaagcgc tgacggtgag aatcgtcagc agcgacacca
     5581 tcggcgcaat gggtcccttg accagaatac cggcactttg tgcgagccag aagccggcga
     5641 cgcagctcaa cggcaccgct tttccggacc gggccgcaaa ataaatcagc gccaggcagc
     5701 cctgtgccgt gacgacggtc gacagcagta ccgtgtccgt aatggcggcg tgcgagacaa
     5761 agttcatcag cggacaggcc gccagaaaag ccgccccgaa aaaggcggtg cgctccggca
     5821 ggaatttccg cgcaaagctg aacagtgcga ggaccgccag tgtgctgcca agcactgatg
     5881 cgagccggta aggccagatg acctccggat gtccggtcag attggccagc ccggcctgca
     5941 gccagtaaat gccgatgggc tttttattgc gggcctgttc catgaaacgg atttgaacgt
     6001 aatcgcccgt ttcggtcatc tgccgcgaag cctgtgcata tcgcggctcg tcgcggtcaa
     6061 tcggcggaat cgtcgtcatc cccggcagat agagcgtcag acaaagaaat aacagagccg
     6121 catagcgtaa ccataccggc gagtccggtt tgtcacatcc cgctttttga tcaattttat
     6181 tcaagttcac tatggttcag gtttaacaga gtgccctctg cattgtcaac gtgacaggga
     6241 tttgatctgc cgccgggccg ctgcagtcca aacgtcttga cctgaacctg cctgcagtgt
     6301 ttattctgct cttatgagat ctgctttaaa cgacctgaaa atcccgcttt tatctctcct
     6361 gctgaccggc ctgatggcgg cctttttttt accgcctatg ccgatcgacg aaacccgcta
     6421 cctgagcgtc gcctgggaaa tgcagcacgc cggttcctac gtggttccgt tgctgaacgg
     6481 cgaagcctac tcacataaac cgccgtttct gttctggatg cttcaggccg gctggaaact
     6541 gttcggcgtc aatgatgtga caccccgcct gaccatctgc ctgttcggaa tgctctgcat
     6601 cctgctcctg cgcaaaatca gcctccggct gtggccggaa gaaaagaaaa ccgccgcctg
     6661 cgccgctctc gtgctggggt ccaccctcat ctggatgctg tggtcctgtg ccatcatgtt
     6721 tgatgtcatg atcaccgtct gggtgctgat ttccatactc ggaatcctgt cggcagagac
     6781 cgcgccgaaa aaaggctgga tcctgctgac gctcggcatt gcgggcggca tgctgaccaa
     6841 agggccggcc atcatggtct acacgctgcc gctggtgctt ctgcgtccgc tgtgggccgc
     6901 ggaccgcgcc gtacgctgga acctgctgac ggcctttctg gccggaaccg gcatcgccct
     6961 gctgtgggcc gttccggcgg ccattctggg cggcgaagaa taccgcaacg ccatcttctg
     7021 gggacagacc gtaaaccgga tcggctcttc gtttgcgcac cgccggccgc tctggtggta
     7081 cgtcccgatt ctgccgctcc tgttcctgcc gtggatattt ttccggccgg cccttgcaaa
     7141 aattgacttc aaaaaagccg accgagggat ccggctggcg gttctctggc tggcggttcc
     7201 gttcgtcata ttctcgctga tcagcggcaa gcaggttcat tatctggttc ccatcatccc
     7261 ggccggcgca ctgctgatgg gccggaatat cgcccggtca gaaaacctga ccggcaccgc
     7321 atgcgtcaaa gtcatcggcg gcttttactg cctgatggga atcgttgcgc tcgtgctgcc
     7381 gtttattcat ttcagcgaaa aaatcggcca gctggatcag ggttcaacct ggattgccgc
     7441 aggcggactg ttctgcgcgg gagttctgct catgcgtccg ttccgctcga ccggcagcgc
     7501 cgccaaagca ctcgccctca gcaccgtcat cattctgctg ttcggactcg ttgaagccaa
     7561 ccgaagcttc gaaggctatg acatcaaagg catcgccgtg gccgtcaaag gcaaaatgga
     7621 cgaaggctgc ccggtggccc atatcgggaa atattacgca cagtaccagt tcctcggccg
     7681 actgccccgg cccctcaccc tcgtgagcag caaaacggac accgatgcga tcaaccaatt
     7741 tatcgccgac catccaaacg gtgtattaat cagctacctg aagaaaaaag atcagcttcc
     7801 ggaaaacgcg gtcgtttatt accgccagcg gtaccgcggc gaaacctccg tcgtgctgtg
     7861 gggtccgccg cgccaacccg ccgcagaaac tgctccatga aaatactact gatcgaagac
     7921 gattcacgga cagcggcttt catcatcaaa gggctgcagc aggaaggttt tgcagtggat
     7981 cgcgccgccg acggcgaaga aggcctgcat cttgcgctcc agaattcatt cgatgtggcg
     8041 gtggtcgata tcatgctccc gaaaatcgac gggctgacgc tgatcgaacg gctgcggaaa
     8101 cagaaattca caaccccgat catcgtcctc agcgcccggt cgtccgttga agaccgcatc
     8161 aaaggccttc acgtcggagc cgacgactat ctcatgaagc ccttcgcctt ctccgaactg
     8221 ctggcccgca ttcaggctct gctgcggcgt gcaggcgcgc agagcgccga cccgacggcc
     8281 ctcaccgtcg aggatctgcg cctcgatctc gtccgccgga aagtctaccg ggccggcaaa
     8341 gagatcgagc tgcagcccaa agaattttcc ctgcttgaat accttatgcg caacagcggg
     8401 cgggtcattt caaaaaccat gatcatggaa cacgtctggg actataactt tgatccgcag
     8461 accaacgtgg tggaggcccg catctgcaaa ctgcgcgata agatcgaccg cgacgcggaa
     8521 aaaaaactga tccataccat ccggggaatg ggctatgtgc tcgaataaac cccggcgccc
     8581 ggcccgctcg gtcgttgccc gcctgacctt cgggtatgcg gtgatctgca ccctgttctg
     8641 cctgaccgtt ttctcggtgg tctccgcgaa aatgagcgcc aatgccagaa aacggatcga
     8701 tcggtccctg acggctgaac tcgccgagct ttcaacgatt taccggcaaa caggcctgaa
     8761 aagcctgcag gatgaattcg atcaggaggc cggactcaac ggggccaaca aactgttcta
     8821 ccggctgctt tcacctgagc agaaacccgt tgccggctcg aatatgagcg gatggaaaaa
     8881 cctcgaagag gaactgcgca ctattccggc gcccaccaat aacggcccgg tcttcagcac
     8941 catttacgcc cgcaacagcc cgttcaatgc ccgcatggct acggtcaaaa ccgcggatgg
     9001 cagcctgctc cagctcggca tcaacctgcg ctccgacaat cattttcagg agaaaatcaa
     9061 ccgggtctta atcgtcagct cgctcatcct gatcgttctg agcacgctct ccggctggct
     9121 gattgcccgg cgcgccatgt ccggcgtcca acgcgtaacc caggccgtcg ccagcatccg
     9181 caaaggccgc ctgaatcagc aggttcccct tggaaatgaa ggacgcgaaa tcgacgaact
     9241 ggcggaagcc ttcaataaaa tgctcagtcg catcgatttg ctcgtgcgcg aacttaaaga
     9301 agtatccgac aacgtggccc acgacctgcg cagtccgatc acccgcatgc gcggggccgc
     9361 cgaaaccaca ctgaccggcc cgcaggaact ggaagcctac cgcgaaatgg gcgtcaccat
     9421 catcgaagaa agcgaacggc tgacgcagat gatcaacacc gcgcttgaaa tcgcccaggc
     9481 agaatccggc atcctcgaaa tcaaccatgc gccgctcgac ctgacggcgg tgttgaaaaa
     9541 tgccgtcgat cttttccaac cggtcgctga aagccggtct ctcaatctgc gcgcagatat
     9601 cccggatgat ccgctcatca tctatggcga caaaatgcgc atccagcgca cagtcgccaa
     9661 cctgctcgac aacgccatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc
     9781 tttttcctgc ttcttcggca ggttgagttt gatgtgcagt tcgcgcagct gtttgagttc
     9841 cacttcgccc ggcgcgccca tcagcaggtc ttccgctttc tggttcatcg gaaaggcaat
     9901 cacttcgcga atgttcggct cgtcggccag cagcatcacc atgcggtcta caccgggagc
     9961 aataccggca tgcggcggcg caccgtattt gaacgcgcgg tgcagcgccg gaaattttgt
    10021 ttcaaccact tcccgtccgt aaccggcgat atcaaatgct ttaatcatca gctcgggact
    10081 gtggttgcga accgcaccgg acgaaagttc ggtgccgttg caaacgatgt cgtactggaa
    10141 agccagcaca tcaagcgggt cttttgtcag caacgcttcc attccgccct gcggcatcga
    10201 gaacggattg tgactgaagg ccactttgcc ttcgtcatcg agttcgaaca tcgggaaatc
    10261 gacgatccag cagaaccgga atacatcttt ttcgagcaga tcgagacggt tgccgagttc
    10321 cgtacgaacc gcgccgccga tgctctgtgc attcttcgcg gtgtcggcaa taaagaacat
    10381 cgtgtcgccg tcgccgactc cgccgagcgc tttgagctgc gcgatttcat ccgcggaaag
    10441 gaatttggcg atcgggctct tcacctcacc gtcaacccaa ctgatgtagc cgaggccttt
    10501 ggcgccgacg gactgggcaa aggcgaccat gtcgtcgaaa aacttgcgcg gctgtccggc
    10561 gcagccttta accgcgatgg ttttcaccac gccgccgctt tccaccacac cggcaaacgc
    10621 cttgaacccg cagttgcgga agatggcgct ggcgtcctgc atgatgagcg gattgcgcag
    10681 gtcgggtttg tccgatccgt atttttccat cgcttcacgg taaggaatcc ggaggaacgg
    10741 aatgccatcg accttcttcg tgctgaactc tttaaaaatc gtcgagagca gtttttcgtt
    10801 caccgcgaac acatcgtcct gagtggcgaa gctcatttcc atatcgagct ggtagaactc
    10861 gccgggcgag cggtcggcgc gagcgtcttc gtcgcggaag cacggagcga tctggaaata
    10921 gcggtcgaag ccggaaacca tcagtagctg cttgaactgc tgcggcgcct gcggcaacgc
    10981 atagaacttg ccggggtgca cgcggctggg gacgagataa tcgcgcgcgc cttccggcga
    11041 cgagctggtg agaatcggcg tctggtattc cagaaagccc tgctccgtca tcagctggcg
    11101 gatgcgcgaa atcactttcg agcgcaggac gatgttatcg tgcaggctct tgcggcgcag
    11161 atcgaggaag cggtatttca gccgcagcgc ttcggaacac tcctcttctt tgttcacctg
    11221 aaacggaatc acttccgatt caccgagcat ttccattgtc tccgcaacca gctcgatttc
    11281 accggtgatg agtttcggat taacagcagc ggtatcgcga gccacgactg agcccgtgaa
    11341 gcagagaaca ctttccacac gccagtgttc aatatttttg taaaacgcct tgtcggggct
    11401 gatcacaatc tgcgtgatgc cgtagtggtc gcgcagatcg atgaacagca cgccgccgtg
    11461 gtcgcggatg gtgttgaccc agccggaaag gcgtacggtc tgtccgacat tttctttgcg
    11521 gagctggccg caggtgtgtg tacgaaattt atgcataagt cagattccgg tttcagttaa
    11581 aaggttcatc agttccggca gttcgacttc gctctgcgag ccgtccgcca tttttttcag
    11641 cacaaacgtg cccttttcga gctctgtatc gccacggata atcaccatct gcgcgccggc
    11701 tttgtcggcc cggcgcatct gcgcctgcac tttgcgcgga gcaagctcca tctcacaggc
    11761 aatgccgcgc tggcgaagcg tctgaagcag aagcagattt tcattcagcg ccgcttcgcc
    11821 gagcgatacg atgaatacag gcggcggcgg agcaaactgc gcggccgtga ttccggtcgc
    11881 ttccagtgaa gcaatcacgc gctccacacc catcgcaaaa ccgacaccgt caatcgagcg
    11941 tccgccgaca tcaatacggt aacgtccgcc gccggccagc gcgttctgcg cgccgaggcc
    12001 gctgtgtacg atttcccaaa ccgtgtggac atagtagtca aggccgcgca ccagtgagct
    12061 gtcgatggac acatcaattt ccagtttacg cagcgtttcg actacggtgt ttaaatacgc
    12121 ccgcgatccg gcgctcataa attccagaac cggcggaaca tccgcaatga cttttttgac
    12181 ggcttcgtct ttggaatcga gcagccgcag cacattggtc tcaaagcgct gctgcgcttc
    12241 ttccggcagc tcgctcagtc gcggacggat cgcttcgcgc aatccttcaa gaaccgcggc
    12301 gcggtcttcc ggcaaaccga tggtgtttaa acggattttt gctccttcaa gcccccaggc
    12361 tttcagcagg tggagttgca gtgcgatcac ttcggcatcg gccagcgggt tcggcgcacc
    12421 aaccgcttcc acgccgatct gatgaaactg gcgcttgcgg ccggcctgcg gccgttcgca
    12481 gcggaacatc gggcccatat aaaaaatacg cgcattcgcg gtttcttcac cgccggaagc
    12541 gacatagcga atggctccgg cagttccttc cgggcgcaaa gcaaatttgc gtccgccgcg
    12601 gtcttccagc gtatacattt ctttggttac aacatcagtc gtgtcgccca gcgaatgggt
    12661 gaaaagttca agcttttcaa ggacaggcgt gcggacttct gaaaaacgat agcgcgcgaa
    12721 aatttcgcgc gcccgctgtt caatcatctg ccagagtgcg acatccggag ctgcaatatc
    12781 ggacatgccc tggatgggtt ggtacgaagt ggaagccatg tctagatgcc ggtaatctgc
    12841 tgtttcatga tcttgccggg tttaaatttt acgacgcgac gctcaggaat cgtcacaacg
    12901 tcttccggct tgttgggatt gcggccgatc cgggctttac ggacgttgat ttcaaaaaca
    12961 ccgaaattgc ggaattcaac cgtttcgcct ttgttgagcg cttcgacgat atagtccaga
    13021 gacttctgga gaatagcggc aacatcct
//
LOCUS       DAOPZO010000043        12904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_5359_length_12904_cov_8.084909, whole genome shotgun sequence.
ACCESSION   DAOPZO010000043 DAOPZO010000000
VERSION     DAOPZO010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 12904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12904
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5359_length_12904_cov_8.084909"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2438
                     /locus_tag="PKI68_04280"
     CDS             <1..2438
                     /locus_tag="PKI68_04280"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNX52940.1"
                     /translation="EERTLFHMRYSRGKDMRSATDFDKFWSFAHVVRDLAVDGFTITQ
                     RNYLDQDVKRLYYLSMEFLIGRLLEQNVMAFGMMDAAREALQRLDIDLQKVLELDIEA
                     GLGNGGLGRLAACFLDSMASMGLPAYGYGLRYEHGIFRQEFEDGWQKERPDNWLELGY
                     PWEMIRPEYTVPVFVYGRIENISSTHRGRHPVWTDWQMFKAVPYDIPVIGYDNNTVNM
                     FRLWSARADEGFRLDVFNQGDYIRAVEEKNWAENVTKVLYPSDSTHAGKELRLIQEYF
                     LCSCSIRDIIRRYQKNHSNFNSFAQKNAIQMNDTHPALAIVELMRILCDENHVPWEKA
                     WEITAKTCAYTNHTLLPEALEKWPVELMERVLPRHLQIIYEINHRFLQRVEMDHPGNT
                     EMIQKVSLIEENGLRQVRMANLAVVGSHKVNGVAGLHSLLLRERVMPEFNQIYPDKFI
                     NITNGITHRRWLLKCNPELSALITDKIGEGWIKDLEELKKLEPFAADKTFQKEFMAVK
                     RRNKVALAQHIEETLHIPIHPESMFDVQVKRLHMYKRQMLSVLHLIALYQRIKADPKI
                     DLVPRTFIFAAKAAPAYHMAKRVIKLINSIGSVINHDPVVAGRLKAVFLPDYNVSLAE
                     KIIPAADLSEQISTAGKEASGTGNMKLALNGALTIGTWDGANIEIAQHVGEENIFIFG
                     HREEELEKLSREGYNPWTFYDSDPELQRVLEAIRVDAFDPTQPDLYMDIFNEFTRHGD
                     PFFYMADFRPYIEAQQKVDALFRQPDIWAEKAILNVARMGWFSSDRTIREYAEKVWNI
                     EPVCIPAPNPIED"
     gene            2750..3247
                     /locus_tag="PKI68_04285"
     CDS             2750..3247
                     /locus_tag="PKI68_04285"
                     /inference="COORDINATES: protein motif:HMM:NF019128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HNX52941.1"
                     /translation="MIRKILLFAVLFSCAALRVQADSCLSSRDREIVAACMVLEAGGE
                     GTDGMRAVLNVILNRAKGDLSRLAPITVKRGAFSCMASIWDNDAPDYSPLFERVMSQT
                     DAYAKASQLIAAMEMGLLTDNTGGATHYHANSVQPYWVSDMRYLTTIGNHIFYVERGS
                     QVALL"
     gene            complement(3329..4348)
                     /locus_tag="PKI68_04290"
     CDS             complement(3329..4348)
                     /locus_tag="PKI68_04290"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNX52942.1"
                     /translation="MFRSNEPEAAGKPEHVDEAVSRPPGLLFTFPTPQTRLDDTNNPE
                     VFMPTESGKIESAWYGSTRTRKFGKLFLPAFHEGVDIAPTERDSKGRAKDPVFAVADG
                     RVGYISRIAGNSSYGIYVVLLHKDTVGEYYTLYAHLASARADLKEGDAVTRGTEIGRI
                     GNTSTLGIPVQRSHLHFEFGTMLNDRFSGWFRKEKLKPFHGNMHGYNLAGLNPMELFP
                     FMTNGLNFVFQQYITNCPVAFRLIVPVTEKPDYYCRYPSLWAGGEPSTGAMVVDVSEG
                     KVPLQARAATEEEAASLQKHKPRVLEVFPEVLGRNGSRLIVENGGRWVPGRNGEQWLD
                     IFLYR"
     gene            4542..5579
                     /locus_tag="PKI68_04295"
     CDS             4542..5579
                     /locus_tag="PKI68_04295"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNX52943.1"
                     /translation="MKRNLLIATVCSLLLGGLLWFRAQTVLNPSWEQPAMSSSARITL
                     SGRFNATRLKALRRQIDTALEESKQIVSIWDENTEISRFNRFNSTEPFPVSAEFARVV
                     QFALDFSALTGGAFDPTVKPLVDRWGFGPKRNSEPPEEIMDAVGWHKVRLENGALVKK
                     HPRLQLDLAGLADGYGADCAAAVIRKSGCTNFLVEVGGGEIVAEGVNRSGNPWRAGIE
                     SPDPDKAPGEEIVLTMEVAGKALTTAGDYRNFKIRDDGTRYSHIIDPHSGKPAESDVA
                     SVTVIAAHGIDADALDTALCVMGSEKGLRWLETHPEFQAFFILHSSNGTFTVKATPGF
                     PGTGSPVAKSQ"
     gene            5648..6430
                     /locus_tag="PKI68_04300"
     CDS             5648..6430
                     /locus_tag="PKI68_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HNX52944.1"
                     /translation="MKLLRSKKAFICDMDGVIYHGSRLLPGVLEFVAWLQNEDKKFLF
                     LTNSSARTLRELSEKLARMGLNVGESHFYTSARATATFLASQKPGGSAYVIGDAGIIN
                     ALYDEGYSINDSNPDYVVVSESANYDYARICHATKLVLNGAKLVGTNPDLTGPIEGGQ
                     IVPATGALLAPIELATGAKAYYVGKPNPLMMRNALKRLGCQREETAIVGDRMDTDIIA
                     GVEAEITTVLVLSGVTKPADIGRFAYRPDYILNGVGDIISGG"
     gene            6488..9250
                     /locus_tag="PKI68_04305"
     CDS             6488..9250
                     /locus_tag="PKI68_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNX52945.1"
                     /translation="MNLHKRIFLRKTQLLKLINGIPGTVYHYRQWKNGRCTFPYSSLD
                     IEELFFATPVELAKNGEIAWDRVCPEFKDLVRNTLNRSAENLKEFEITFRVRSPQGRM
                     QWIRNRAVPERLRDGSTLWYGHMQNVTAQYEAEEGAKQKSALLNVIFETLPDHIYYMD
                     RESRMLGANPACCNHHKKTAEEMIGKTDIELYPNSIGRKLYDEEQQLMTDAQTLRERE
                     KHVDDKGRIIHLESVKIPLRSPSGRIIGLAGISRDITEQVEREEALLQAKHAAEQSTA
                     FIKAIFDNLEDSFYYKDRQSRVLGGNRAWVTSRGAKSIDELIGKTDIDLHPAPLGQQL
                     YDNEQRQMAEGEVTRIRERHIRKDGSIGYVEAVKCPMRDEKGEVIGLAGISRDITRQV
                     ENEKRLVEAQQEAEAANKAKSAFLAMMSHEIRTPMNGVIGAASLLLGTELTAQQDEFV
                     HTIQVSGENLMTIINDILDYSKIEAGKIELEKAPFELRECIEDAFDLFIQAAAKKNVE
                     LLYYVDPDVPKALLGDTTRIRQILVNLLGNAIKFTENGEVTLKVHNVTTDENKRQCQL
                     EFAVHDTGIGIADEHKVRLFEAFTQADASSTRKYGGTGLGLTISRRLTELMDGKIWFE
                     SEKGKGSTFFFTLTLPITDHVDPKAEHLPVEALRGKRALIVDDNETNRWLLSDQLAQW
                     GVLSEAFAEPEKALQHLKNGGRYDIALIDFQMPCMDGSDLAKQIYRIKTDPHIPVIIL
                     SSSCEHIPADPSIRARLSKPVKISKLCDQMLQALAEQRTGSAGEETAGTQIQPKKLRN
                     LRVLVAEDNPINQRIAQMMLQRIGCEKPVIVSDGEEAVAAVMDAAYDVILMDIQMPRM
                     NGLDATRQIRKHTGDPEKPWVIALSAGVMREEQTAALEAGMNEFLSKPLVIEHLEKTL
                     DHVLLS"
     gene            complement(9247..9696)
                     /locus_tag="PKI68_04310"
     CDS             complement(9247..9696)
                     /locus_tag="PKI68_04310"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HNX52946.1"
                     /translation="MKKHRHQFLLPVALLLLGTGCASIRPPRGVPPRTVTIEATGYCD
                     CGQCCSWERVWWAPWRTVYSSGPNKGQPKKVGITASGTKVHDGTIAADRRYYPFGTIM
                     YIPGYGYGRVEDTGSAIQGPARIDLFFSSHQQALEWGRKRSSVKVWR"
     gene            complement(9683..12805)
                     /gene="ileS"
                     /locus_tag="PKI68_04315"
     CDS             complement(9683..12805)
                     /gene="ileS"
                     /locus_tag="PKI68_04315"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNX52947.1"
                     /translation="MFKPVASKVDFAGMEEGILAFWQEHGTFKKSVENRSKNSEFVFY
                     DGPPFATGLPHYGHLLAGTIKDIVPRYQTMRGHRVERRFGWDCHGLPIEALAQDALGL
                     AGAPAIVEAGVGVFNEQCRSMVTKYVEEWEKTVTRMGRWVDFKNDYKTMDKDFMETIW
                     WVFSELWKQGRIYRAHRIMPYSWKLNTPLSNFEAGRNYQDVQDPAITVRVKLKGFEIA
                     NACALVWTTTPWTLPSNLAICAGADIDYVAVRDAESGEVYVLAEARLGAYYKKEAEYE
                     ILRKFKGRHLEGLTYEPLFDFFKDNPRSFRVLLDNFVSTEDGTGIVHMAPAYGEDDYR
                     ICRAADIPLVEPLDEDCKFTAQVPDYKGIFCKDADKPIIKRLKDEGKLVHQSTIQHSY
                     PFCERTDTPLIYRAIDAWYVRVEDLRDEMLANNAQVHWMPEYVGEKRFANWLADAKDW
                     NISRNRFWGSCIPVWVNVADPSDTICISSVDELEKLSGQKVTDLHKHNLDGILIKKDG
                     KTYKRTPEVLDCWFESGAMPYAQQHYPFENKAHFEANFPAHFIAEGLDQTRGWFYTLM
                     VLSTALFKKPAFRNVVVNGLVLAEDGLKMSKRLKNYPDPLHVVHEYGADALRLYMINS
                     PVVKAESLRFSEEGVKHALRHLLIPLWNSYSFFVTYANIDKWKPGKSKPGQSANLLDR
                     WIESSLANLCQEVTAAMDNYDLQRAVRPFVAFIEDLTNWYIRRSRRRFWKSEDDGDKQ
                     QAYATLYHALIELCKIAAPFTPFIAEEIYRNLRTDEMPESVHLCDFPTAQGAKRDVAL
                     ETQMRIVMDVVSMGRQLRKDRDVKVRQPLRRLDVVSRDSGLLNQVGELKEIIAEELNV
                     REVNFDDDETSLATLSAKANFKTLGKKFGKQVQQINKVLSTLSLEELKVAAAGKGLVL
                     KDGDQAFDLAGEDIIVERTPKAGLAVQAQGALVAALDLELDDDLVREGLARELVNNVQ
                     QMRKAHGFEVTDRIHLTLSSDEAVREAVLVFDDYIRSEVLALSVETIETEGETVDLNG
                     HACTIALEKA"
BASE COUNT         3107 a         3561 c         3479 g         2757 t
ORIGIN      
        1 tcgaggagcg cacgctgttc catatgcgct acagccgcgg caaggatatg cgcagcgcga
       61 cggatttcga caagttctgg agcttcgccc atgtcgtgcg agacctggct gtcgacggct
      121 tcaccatcac ccagcgcaat tatctcgatc aggatgtaaa gcgtctttat tacctctcga
      181 tggaattcct gatcggcagg ctgctggagc agaatgtgat ggctttcgga atgatggatg
      241 ccgcacgcga agccctgcag cggctggaca ttgatttgca gaaggtgctt gagctggaca
      301 tagaagccgg tttaggcaac ggcggccttg gacggctggc cgcctgtttt cttgattcga
      361 tggcttccat gggactgcca gcctacggct acggcctgcg ttatgaacac ggaattttcc
      421 ggcaggagtt cgaggacggc tggcagaaag aacggccgga caactggctg gaactgggct
      481 atccgtggga gatgatccgc cctgaataca ccgtgccggt gtttgtgtac ggacgtattg
      541 aaaatatttc cagcacccac cgcggacgcc atccggtctg gaccgactgg cagatgttca
      601 aagccgtgcc gtacgatatt ccggtgatcg gctatgacaa caacaccgtc aatatgttcc
      661 gcctctggag cgcccgcgcc gatgaaggct tccgccttga tgtcttcaat cagggcgact
      721 acatccgcgc cgtagaagaa aaaaactggg ccgaaaatgt caccaaggtt ctctatcctt
      781 ccgacagcac ccacgccggc aaagagctgc ggctgattca ggaatatttc ctttgcagct
      841 gctcaatccg cgacatcatc cgccgctacc agaaaaacca cagcaacttt aattcgtttg
      901 cacaaaaaaa cgccatccag atgaatgaca cccatccggc gctggctatc gtcgagctga
      961 tgcgcatatt gtgcgacgaa aatcatgttc catgggaaaa agcatgggaa atcaccgcaa
     1021 aaacctgtgc ttacaccaac cacaccctgc tgcccgaagc gctcgaaaaa tggccggttg
     1081 aactgatgga acgcgttctg ccgcgccatc tgcaaattat ctacgaaatc aaccaccgct
     1141 tcctgcagcg tgttgaaatg gatcaccccg gcaacaccga aatgattcag aaagtttcac
     1201 tgatcgaaga aaacggtctc cggcaggtcc gcatggcgaa tctggccgtg gtcggcagcc
     1261 acaaagtcaa tggcgtggca ggtcttcatt ctctgctgct gcgcgaacgc gtcatgccgg
     1321 aattcaatca aatctatccg gacaaattca tcaacatcac caacggcatc acccaccggc
     1381 gctggcttct taaatgcaat ccggagcttt cagcgctcat cacggataag atcggcgagg
     1441 gctggatcaa agacctcgaa gagctcaaaa agctggagcc ttttgcagcc gataaaacct
     1501 ttcagaagga attcatggcc gtcaagcgcc gcaacaaagt ggcactggca cagcacattg
     1561 aagaaacgct gcatataccg atccatcccg aatcgatgtt cgatgttcag gtcaaacgcc
     1621 tgcatatgta taaacggcag atgctcagcg tcctgcacct catcgcactg taccagcgaa
     1681 tcaaagccga cccgaagatc gacctcgtgc cgcgcacatt tatctttgcc gcgaaagccg
     1741 cacccgccta tcacatggcg aaacgcgtca ttaagctcat caactccatc ggctcggtca
     1801 tcaaccatga tccggtcgtc gcaggccgcc tcaaagccgt tttcctgccg gactataacg
     1861 tttcgctggc cgagaaaatc attccggcgg cagacctctc cgagcagatt tcgacggcgg
     1921 gcaaagaagc ttccggaacc ggcaacatga aactggctct caacggcgcg ctcaccatcg
     1981 gcacctggga cggtgccaac atcgaaatcg cccagcacgt cggcgaagaa aatatcttta
     2041 tcttcggcca ccgcgaagag gaactcgaaa agctttcacg cgaaggctac aacccgtgga
     2101 cgttctacga cagcgatccc gaattgcaac gagttcttga agccatccgt gtcgacgcct
     2161 ttgacccgac acagccggat ctctacatgg atattttcaa cgagttcacc cgccacggcg
     2221 atccattctt ctacatggcg gacttccggc cgtacatcga agcacagcag aaagttgatg
     2281 cgctgttccg tcagccggat atctgggccg aaaaggccat cctcaacgtc gcgcgcatgg
     2341 gctggttttc cagtgaccgc acgatccgcg aatacgctga aaaagtctgg aacattgaac
     2401 cggtctgtat tcccgcaccg aacccgattg aagactgacc tgtattcaac atcgcaaatc
     2461 cggacaaaac accggtattt cgcttactct ccggcatttc tgtattccgg gttcagccct
     2521 ccgtcagctg aaaaacgagc cgggaaccca gcttgttttc tcatcccgga atctgcttat
     2581 ctcctttaaa acaagggaaa aatcgcagtt gtcatccccc gccgctccaa gtgtggcata
     2641 aaaaatgaga aaatttacct tttaccgcat ctcctgcgca ggcacaattt tccgctcttt
     2701 ttcaaaacgt gccgtctcgg gggagattta aagcacaggg ggaagaagca tgatacggaa
     2761 aattttactt tttgccgttc tgttcagctg cgctgcactt cgcgtacagg ccgacagctg
     2821 cttaagcagc cgtgaccgcg aaattgtcgc cgcctgcatg gttctggagg ccggcggcga
     2881 aggaaccgat ggtatgcggg ctgtcctgaa tgttattctg aaccgtgcaa agggcgactt
     2941 gagccgctta gctccgataa cggtgaaacg cggcgctttc tcctgcatgg ccagcatctg
     3001 ggataatgat gcgccggatt acagccctct gttcgagcgt gtcatgagcc agacggacgc
     3061 ttatgcaaag gcctctcaac tgattgccgc catggagatg ggactgctga ccgacaacac
     3121 cggcggagcc acgcattatc acgccaactc cgttcaacct tactgggtca gtgacatgcg
     3181 ctacctcacc accatcggaa atcacatttt ctatgtcgaa cgcggcagtc aggttgccct
     3241 gctctgacat aagacagcag ttgatcaaac agaagaggtc gggcgtccgg cccggccgcg
     3301 gcagccaggc ctgaatcacc ggctgatttt accggtacag aaaaatgtcc agccactgtt
     3361 cgccgttacg gcccggcacc caccggccgc cgttctcaac aatcagccgg gagccgttac
     3421 ggcccagaac ttccggaaag acttccagaa cgcgcggttt atgtttctga agcgatgcgg
     3481 cttcttcttc cgtcgctgcc cgggcctgca gcggaacttt accttcagaa acgtccacaa
     3541 ccattgcacc ggtcgaaggt tcgccgccgg cccacagcga cggatagcgg cagtaatagt
     3601 ccggcttttc cgtaaccgga acaatcagcc ggaacgcgac gggacagttc gtgatgtact
     3661 gctggaaaac aaagttcagg ccgttggtca tgaatggaaa cagctccatc ggattgagtc
     3721 cggcaaggtt gtagccgtgc atgttaccgt gaaagggttt cagcttttct ttgcgaaacc
     3781 agccgctgaa ccggtcgttc agcatggttc cgaactcgaa gtgcaggtga ctgcgctgaa
     3841 caggaattcc aagtgttgag gtgtttccga tccgcccgat ttccgttccg cgcgtcacag
     3901 cgtcgccttc tttcaagtcg gcccgggccg aagcaaggtg tgcataaagc gtgtagtatt
     3961 cgccgacggt gtctttgtgg agcagtacga cgtaaattcc gtatgaggag tttccggcga
     4021 ttcggctgat gtagccgacg cggccgtcgg caacggcaaa taccggatct ttcgcccggc
     4081 cttttgagtc gcgttcggtc ggcgcgatgt ccacgccttc atggaacgcc ggaagaaata
     4141 atttgccgaa cttccgggtg cgggttgagc cgtaccaggc cgattcaatt ttcccggact
     4201 cggtcggcat aaacacttcc ggattattgg tgtcatcgag acgggtctgc ggtgtcggaa
     4261 aggtaaatag cagtccaggc ggccgggaaa cggcctcgtc gacgtgttcc ggctttccgg
     4321 ccgcttccgg ctcattactg cggaacatcc agcatccggc ggcagtcagg ctgattgcga
     4381 caagaacgaa gagcgtgcgg aaaaatgagg tgcgttgacc ggcgtctgga agagtgtatg
     4441 gcataaatta agcggcgcat ggtgccagac tctttcataa aaacaaccgt aaaccttttc
     4501 tgcggttgca gtttttttta aaacaggaaa actcagcggt tatgaagaga aatctgctca
     4561 ttgcaaccgt gtgttcgctg ctgcttggcg gcctgctgtg gttcagagct caaacggttt
     4621 taaatccgtc atgggagcag ccggcaatga gttcgtccgc ccgcataact ctttccggaa
     4681 ggttcaatgc aacccggtta aaggcgcttc gcagacagat cgatactgcg cttgaggagt
     4741 ccaaacagat tgtctccatc tgggatgaaa ataccgaaat ctcccggttc aaccgcttca
     4801 attccactga acccttcccc gtttcagctg agttcgccag agtcgtccaa ttcgcattgg
     4861 atttcagcgc gctgaccggc ggcgcatttg atccgacggt aaaaccgctg gtggatcgat
     4921 ggggcttcgg gccgaaaagg aattcggaac cgccggaaga gatcatggat gccgtcggct
     4981 ggcataaagt gcggctggaa aacggtgcgc tcgtcaaaaa gcacccgcgg ctccagctgg
     5041 atcttgcggg gctcgctgac ggatacggcg cggattgcgc cgccgccgtc atcagaaaaa
     5101 gcggatgcac caactttctt gtcgaagtag gcggcggtga aatcgttgcc gaaggcgtca
     5161 accgttcagg caatccgtgg cgcgccggaa tcgagtcgcc ggatcctgat aaagcgcccg
     5221 gcgaagaaat tgttctgacg atggaggtcg cagggaaagc actgacaaca gccggtgatt
     5281 accggaattt taaaatccgc gatgacggca cgcgttattc gcatattatc gatcctcact
     5341 ccggaaagcc cgcggagtcg gacgtggctt ccgtaaccgt cattgcggcg catggcatcg
     5401 atgccgacgc cctcgatacc gccctttgcg taatgggttc cgaaaaagga cttcgctggc
     5461 tggaaaccca tccggaattc caggcgttct tcattcttca cagcagtaac ggcacattca
     5521 ccgtaaaagc cacccccgga tttccgggaa cgggttcccc tgtcgcaaaa tcgcagtaaa
     5581 aacccttggt ttccttctgt gaaaaatccg gttcagataa taccatgcgg ttgaaaagga
     5641 gttgtttatg aagttgttgc ggagcaagaa ggcgtttatc tgcgatatgg acggcgtgat
     5701 ctaccacgga agcagactgc ttccgggcgt actcgaattt gtcgcgtggc ttcagaatga
     5761 ggataaaaag tttctgtttc tgaccaacag cagcgcacgc acactccgcg agctgagcga
     5821 gaagctggcc cgcatgggac tgaatgtagg tgaaagtcac ttctacacca gcgcacgggc
     5881 cacggcgacg tttcttgcca gccagaagcc cggcggcagc gcgtatgtga tcggtgatgc
     5941 aggcatcatt aatgcacttt atgatgaagg gtattcgatc aatgattcca acccggacta
     6001 tgtggtggtg agtgaaagcg cgaactatga ttacgcccgc atctgccacg ccaccaaact
     6061 ggtgctcaac ggcgcgaaac tggtcggaac caatcccgac ctgaccggcc cgatcgaagg
     6121 cggacagatt gttccggcca ccggcgccct gctcgcgccg attgagctgg caaccggcgc
     6181 caaggcctat tacgtcggca aaccgaatcc cttaatgatg cgtaatgcgc tgaagcgtct
     6241 tggctgtcag cgcgaagaaa ccgccatcgt cggcgaccgg atggataccg atatcatcgc
     6301 gggcgtcgaa gcggaaatca cgaccgtgct ggtacttagc ggtgttacca aacctgctga
     6361 tataggccgg tttgcctacc ggccggatta tattctgaac ggcgtcggag atattatttc
     6421 cggcggttaa gttcttttaa ataccgattg aacacaagga tatcggaagg tagtctccgc
     6481 actacaaatg aacttgcaca agcgtatatt tctaagaaag acacagctgc tgaaactgat
     6541 caacggtatc cccggaaccg tttatcatta ccgccagtgg aaaaacggac ggtgcacgtt
     6601 cccctactca tcccttgata ttgaagaact atttttcgca acacccgtcg agctcgcaaa
     6661 aaatggcgag attgcatggg atcgcgtctg tccggaattc aaagatctcg tgcggaatac
     6721 gctgaacagg tctgcggaga atctcaaaga attcgaaatt actttccgcg tccgttctcc
     6781 gcagggccgt atgcaatgga tccggaaccg cgctgtgccg gagcggctgc gtgacggaag
     6841 caccctgtgg tacggccaca tgcaaaatgt caccgcacag tacgaggctg aagaaggagc
     6901 caaacagaaa tccgcactgc tgaacgtgat tttcgaaact ctgccggatc atatctatta
     6961 catggatcgt gagtcccgga tgctcggcgc aaatccggcc tgctgcaacc atcacaagaa
     7021 aacagccgag gaaatgatcg gtaaaaccga tatcgaactc tacccgaaca gtatcggcag
     7081 aaagctgtac gatgaagagc agcagctgat gaccgatgca cagaccctga gggagcggga
     7141 aaaacatgtc gatgacaaag gccgcattat ccacctggaa tctgtaaaga ttccgctgcg
     7201 cagtccttcc gggcggatta tcggtctggc cggtatctcc agagatatca cggagcaggt
     7261 cgaacgcgaa gaagcactgc ttcaggcaaa acatgcggcg gagcaaagca ccgctttcat
     7321 caaagccatt tttgataacc tggaagacag cttttactat aaagaccggc aatcccgtgt
     7381 actgggcggc aaccgggcct gggtaacgtc gcgcggcgcc aaatcaattg acgagctgat
     7441 cggcaaaaca gatatagacc ttcatcccgc cccgctggga cagcagctgt atgataatga
     7501 acagcggcag atggcagaag gtgaagtcac acgcatccgc gaacgccata tccggaaaga
     7561 cggcagcatc ggatatgtcg aggcggtcaa atgtccgatg cgggatgaaa aaggtgaagt
     7621 gatcggtctc gccggcattt cccgcgacat cacccggcag gtcgaaaatg aaaaacgttt
     7681 ggtggaagcc cagcaggaag cggaagccgc aaataaagcc aaaagcgcgt ttctggccat
     7741 gatgagtcat gaaatccgca ctccgatgaa cggcgttatc ggcgcggcca gtcttttgct
     7801 gggaacggaa cttaccgcgc agcaggacga atttgttcac acgattcagg tcagcggtga
     7861 aaacctcatg accatcatca atgacattct cgactattca aaaatcgaag ccggaaagat
     7921 cgagctggaa aaagctccct tcgaactgcg cgaatgcatc gaagacgcgt ttgacctgtt
     7981 catacaggcc gccgcaaaga aaaacgtaga actgctctac tacgttgatc cggatgtccc
     8041 caaggctctg ctcggcgaca caacccgcat acgccagatt ctggtcaacc tgctcggcaa
     8101 tgcgatcaag ttcacagaaa acggagaggt taccctcaag gttcacaacg tgaccaccga
     8161 tgaaaataaa cggcagtgcc agcttgagtt tgccgtgcac gacaccggca tcggcatcgc
     8221 tgacgaacat aaagtacgcc tgtttgaggc cttcactcag gcagacgctt ccagcacccg
     8281 taaatacggc ggaaccggac ttggactgac catcagccgc agattaaccg aattgatgga
     8341 cggaaaaata tggtttgaaa gtgaaaaagg taaaggctcc acattcttct tcaccctcac
     8401 actgccgatt acagaccatg tcgacccgaa agcggagcac ctgcccgtcg aagcgctgcg
     8461 cggcaaacgc gcactgattg tggatgacaa tgaaaccaac cgctggctcc tcagtgatca
     8521 gctcgcgcaa tggggtgttc tttcggaagc gtttgcagaa cctgaaaaag cacttcagca
     8581 tctgaaaaac ggcggcaggt atgacattgc cttgatcgac ttccagatgc cgtgcatgga
     8641 cggcagtgat ctggcgaaac aaatttaccg gatcaaaacc gatccgcaca ttccggtaat
     8701 tatactcagc agttcctgtg aacatattcc ggccgacccc tccatcagag cccggctctc
     8761 caaaccggtc aagatcagta aactctgcga tcagatgctt caggcgctgg cggaacagcg
     8821 gaccggatcg gccggggaag aaaccgccgg aacacagatc cagcctaaga aactgcgcaa
     8881 tctgcgcgtt ttggttgctg aagacaatcc catcaaccag cggattgcgc agatgatgct
     8941 ccagcgaatc ggctgtgaaa aacctgtgat cgtttcggat ggcgaagaag ctgtcgctgc
     9001 cgtcatggat gccgcatatg acgtaattct gatggatatt cagatgccgc gcatgaacgg
     9061 gctggatgcc acccgccaga tacgcaaaca caccggcgat cctgagaaac catgggttat
     9121 cgcgttgtcc gcaggggtta tgcgtgaaga acagaccgcc gcactagaag ccggaatgaa
     9181 tgaatttctt tccaaaccgc tcgttattga gcatttggaa aaaacgctcg atcacgtcct
     9241 gttatcctag cgccagacct tgaccgaact tcgtttgcga ccccattcaa gcgcctgttg
     9301 atgcgagctg aaaaaaagat cgatccttgc cgggccctga atggcactgc cggtatcttc
     9361 cacccggccg tagccgtacc ccggaatata catgattgtg ccgaacggat aataacgccg
     9421 gtcggcagca atggttccat catgcacttt ggttcctgaa gcagtgatgc cgactttttt
     9481 cggctgtcct ttattcggac cggaagaata aaccgtccgc cacggagccc accagacgcg
     9541 ctcccagctg cagcactgac cgcaatcaca gtagccggtc gcctcaatcg tcaccgtccg
     9601 cggcggaact ccgcgcggag gacgaatgga agcgcaacct gttccaagca ggagcagcgc
     9661 gacagggagc aggaattgat gcctatgctt tttcaagggc aatcgtgcag gcgtgaccgt
     9721 tgagatcaac cgtttcgcct tccgtttcga tggtttcaac cgacagagca agaacctccg
     9781 agcggatata gtcgtcaaaa accagtacag cttcgcgtac ggcttcgtcc gaagacaggg
     9841 tgagatgaat ccgatccgtc acttcaaagc cgtgcgcttt gcgcatctgc tggacattat
     9901 taaccagttc gcgggccagc ccttcccgaa caagatcgtc gtccagttca agatcaagag
     9961 ctgcgacgag tgcgccctgc gcctgcacgg cgagcccggc cttcggcgtg cgctcgacga
    10021 tgatatcttc tccggcgaga tcaaaggcct gatcaccatc cttaagcacc agacctttgc
    10081 cggctgcagc gaccttcagt tcttcaagcg aaagcgtcga cagaacctta ttgatctgct
    10141 ggacctgctt tccgaacttc ttaccgagcg tcttgaagtt cgctttcgcg ctgagcgtgg
    10201 caagactggt ttcgtcgtca tcgaaattca cttcacggac attgagttct tcagcgataa
    10261 tttctttaag ttcgccgacc tgattgagca gtccgctgtc gcggcttacg acgtcaagac
    10321 ggcgcagcgg ctgacggact tttacgtcgc ggtctttacg aagctggcgg cccatgctga
    10381 caacatccat gacgatacgc atctgggttt ccagcgcgac gtcgcgcttg gcaccttgag
    10441 cggtcgggaa atcgcagaga tgcaccgact ccggcatctc gtccgtgcgc aggttgcggt
    10501 aaatctcttc cgcaatgaac ggcgtgaacg gcgcggcgat tttgcacagt tcgatcaacg
    10561 cgtgatacag cgtggcataa gcctgctgct tgtcgccgtc gtcttcgctc ttccagaagc
    10621 ggcggcgcga gcggcggata taccagttcg tcaggtcttc gatgaacgcc acaaacgggc
    10681 gaaccgcgcg ctgcaggtcg tagttatcca tcgccgccgt cacttcctga cagagattgg
    10741 ccagtgaact ttcgatccag cggtcaagca ggttggcgct ctgccccggc tttgattttc
    10801 cgggtttcca tttatcgata ttggcgtagg taacgaaaaa gctgtaggaa ttccaaagcg
    10861 gaatgagcaa atggcgaagc gcgtgtttga cgccctcttc ggagaagcgc agcgactcgg
    10921 ctttgacaac cggagagttg atcatgtaaa gacgcagcgc gtcggcgccg tattcatgaa
    10981 caacgtggag cggatcggga tagttcttga gccgcttgct cattttcagt ccgtcttcgg
    11041 ccagcaccag accgttaaca acaacattgc ggaaggccgg ctttttaaac agcgcggtgg
    11101 aaagaaccat gagcgtgtag aaccagccgc gggtctggtc gagtccttcg gcgataaagt
    11161 gagccgggaa attggcttcg aagtgcgcct tgttttcaaa gggataatgc tgctgagcgt
    11221 acggcatggc accggactca aaccagcagt cgagaacttc cggcgtacgc ttgtaggtct
    11281 tgccgtcctt tttgatcaga atgccgtcga gattgtgttt atgcaggtcg gtgactttct
    11341 gtccggaaag cttttcgagc tcgtccacgg acgaaataca gatggtgtcg gacggatccg
    11401 ccacattgac ccagaccgga atacaggaac cccagaaacg gttgcggctg atgttccagt
    11461 ccttggcatc agccagccag ttggcgaagc gtttttcacc gacgtattca ggcatccaat
    11521 gcacctgcgc attattggcg agcatctcgt cgcgcagatc ttcgacgcgg acataccacg
    11581 catcaatggc gcgatagata agcggcgtgt cggtgcgctc acagaacgga tagctgtgct
    11641 ggatagtgga ctggtgaacc agcttccctt cgtccttgag ccgtttgata atcggcttgt
    11701 ctgcgtcttt gcagaaaatc cctttatagt ccggaacctg cgcagtgaat ttgcaatctt
    11761 cgtcgagcgg ttccaccagc ggaatatcgg cagcgcggca gatgcggtaa tcatcttcac
    11821 cgtaagccgg ggccatatgc acaataccgg ttccgtcttc ggtgctgaca aaattatcga
    11881 gcaggacgcg aaatgagcgg gggttgtctt tgaaaaagtc aaagagcggc tcataggtca
    11941 gcccttcaag atgtctacct ttgaattttc tgaggatttc gtattccgcc tcttttttgt
    12001 agtaagcgcc gaggcgcgct tcggccagca cataaacctc gccgctctcc gcgtcgcgca
    12061 cggcaacgta gtcaatatcg gctccggcgc aaatggcaag attggacggg agcgtccacg
    12121 gagtggtcgt ccagaccagc gcgcaggcgt ttgcgatttc aaagcccttg agtttcacgc
    12181 ggacggtgat ggcaggatcc tgaacatcct gatagttgcg gccggcttca aagttagaga
    12241 gcggcgtatt gagtttccag ctgtagggca tgatgcggtg ggcgcggtaa atgcggcctt
    12301 gcttccagag ttcggaaaac acccaccaga tggtttccat gaaatcttta tccatggtct
    12361 tgtaatcgtt cttgaaatca acccagcgac ccatgcgggt aacggtcttt tcccattctt
    12421 cgacatattt ggtgaccatt gagcggcact gttcattgaa aacgccgacg cccgcttcga
    12481 cgatggcggg cgcaccggcc aggccgagag cgtcctgagc cagtgcttcg atcggcagtc
    12541 cgtggcagtc ccagccgaaa cggcgctcga cgcggtgccc gcgcatggtc tggtagcgcg
    12601 gaacaatatc tttgatggtg ccggcgagca gatgaccgta atgcggcagg ccggtggcaa
    12661 acggcggacc gtcatagaaa acaaactcgg aatttttaga acggttttcg accgactttt
    12721 taaacgtgcc gtgctcctgc cagaaggcaa gtatgccctc ttccatccct gcaaaatcaa
    12781 ctttactggc caccggtttg aacatgtcgc tacttcctga ataaaagcaa actatgcact
    12841 gctggaaatt ggagcgggag acgggagtcg aacccgcgac gtcaagcttg ggaagcttgc
    12901 attc
//
LOCUS       DAOPZO010000044        12789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_5430_length_12789_cov_9.966232, whole genome shotgun sequence.
ACCESSION   DAOPZO010000044 DAOPZO010000000
VERSION     DAOPZO010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 12789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12789
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5430_length_12789_cov_9.966232"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PKI68_04320"
     CDS             complement(<1..464)
                     /locus_tag="PKI68_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNX52948.1"
                     /translation="MFIQQALTDPFLYFGWITFVVFSICCHEYAHAHTALRFGDDTAA
                     RNGHLTLNPLVQMGLQSLIVLALFGLAWGAVPVNPVSVRSALKRALIALSGPLMNLLL
                     CFVFSLLMVIAEIAGSSSAEKFLLIGGAVNGMLFVFNILPVPVLDGFAVVSA"
     gene            complement(471..1730)
                     /locus_tag="PKI68_04325"
     CDS             complement(471..1730)
                     /locus_tag="PKI68_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNX52949.1"
                     /translation="MNGVDFNHLAGSLAALLIAGFWAVLFAAFRETGNAGVLRLAEKK
                     PQARAALESWAGRWNLLRTTLRFGLTFFEISAIYFAFHAFNFPLIAEWAGLMLVMTPL
                     YLLFIRVIPYVMAESYADRLSLFFLPVVIGLTRLLFPLVWPVYMLERGLLARALSSAD
                     EDDSPTPEEEIMTLVDQADDEELEKEERDIIRSVFEFGDTVVREIMTPRVDIEGLRAG
                     STVAECVEKVQKSAHSRFPVYEETIDNVAGVVHVKDLLRLFARGEETHPVAELVKKLN
                     AVPETMPITDLLRQMRSQHFHMALVVDEYGGTAGIVCIEDVIEELIGEIRDEYDLAER
                     DILKCSDGTALVKAGMAVADLNEELGLHIPESEEYDSIGGYVLSELGTIPPAGTKLAA
                     PGLEITVQNATPRRIHTLLLTPVPEKE"
     gene            complement(1732..2190)
                     /gene="ybeY"
                     /locus_tag="PKI68_04330"
     CDS             complement(1732..2190)
                     /gene="ybeY"
                     /locus_tag="PKI68_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNX52950.1"
                     /translation="MKTALINRQKRRINLPAIRRLTAELAAALEAADPETVWQEVTLL
                     LTDDAGITQYNREFFGKDRATDVISFRGEPVPGEDGATGDLIVNVERAVQEGPRHGGA
                     DAELALYIAHGFNHLSGADDNTPAKRAAMRRTEMRRLSALQTELKNLIAD"
     gene            complement(2120..4336)
                     /locus_tag="PKI68_04335"
     CDS             complement(2120..4336)
                     /locus_tag="PKI68_04335"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNX52951.1"
                     /translation="MKRKLFRKKINRVAATRDVTARKAARHFPLFNLLLAVVLWVALV
                     VFFYSGRLLRPQPLTAGQQSPVTITADIDFLAENLAATQAGQRAAAEAVLPVFSIDTS
                     AIEDARLILTTNQLTEIIPENKQIDVRTVFFTALSNTASRGIISAGDAQTRFNGAAPS
                     GKVVLRGNGTDVIRTLDGFALQPDAIKATVTAIAQNLPRGTRNEEAIRALVAPWLRPN
                     LVYDAAETTSRRTQATGLVDPVIEPVSAGTILVRNGDTVSEQTLKWLTAHERRLSELE
                     TPAERIQRLAGSGLLLLLGLALTVAVAGIVQPALLHSRRKVLLLITLSILPLIIGKGM
                     LYATVNLRLMPPAVLDFIMPLSIAPLLAAILLGSAPGLMVGFWVSFALATLLGGSFDL
                     FVLGLLASAAAVYTTRNVKRRTALFRAGFWIGAVEILFALVMAVLKNPSWQVLLPQLG
                     AAMLCGLISALLAMLLIPVFEHLFKITTDISLLELSDLSHPLLQSLAINAPGTYHHSL
                     MLASLAQNAAEAIGANGLMLRVCAYFHDIGKLVKPGFFSENIQFTENPHDELTPSMST
                     LVIVSHIKEGVTLAKKFKLPQVIIDGIEQHQGTSLVSVFYHRAKTRHQKENAAGQLAI
                     NDEDFRYEGPRPQNREMAILMLADSCEAASRSLGKPTPVRITNLINDLFDARLRDGQL
                     DECDLTLAELNKIKQSFIFSLTNMLHGRVAYPKNENRTDKPAETPDQPAGHPPADR"
     gene            complement(4360..5304)
                     /locus_tag="PKI68_04340"
     CDS             complement(4360..5304)
                     /locus_tag="PKI68_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNX52952.1"
                     /translation="MSEITLHFDNAREAQDIAAHPAGTLEAIERAFDVTLTARDTWLK
                     IEGRGTESAVRFFEALRAARERGFSLNTGGVHYALKSFTEGRENMLETFSAMRIDVSP
                     RKSPVFPRTFGQYIYLDAIKHNPITFGLGPAGTGKTYLAMAAAVSALLKDEVSRIILT
                     RPAIEAGEALGFLPGDLQQKVDPYLRPLYDALHDMLPAEEIQRLMERGIIEVAPLAYM
                     RGRTLNHAFVILDEAQNTTPLQMLMLLTRLGFESRAVITGDPTQVDLPDKKKSGLTEA
                     RHILSKIDGLTFIELDEDDVVRHPLVQKIIRAYRHHRV"
     gene            complement(5364..5570)
                     /locus_tag="PKI68_04345"
     CDS             complement(5364..5570)
                     /locus_tag="PKI68_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNX52953.1"
                     /translation="MAEFTITGEYIELDKLLKAAHLCGTGGEARIVISEGLVTVDGET
                     ELRLRRKVRPGMTVEYDGNRLAVR"
     gene            complement(5563..5817)
                     /locus_tag="PKI68_04350"
     CDS             complement(5563..5817)
                     /locus_tag="PKI68_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52954.1"
                     /translation="MKRDIEWIEKIEDGVKRTVRIAITMNKGIKWQFKRSDEERWDYD
                     TPASPEDWQALEDKVAALYNRRRATHEELELVRAQRKKHG"
     gene            complement(5814..6020)
                     /locus_tag="PKI68_04355"
     CDS             complement(5814..6020)
                     /locus_tag="PKI68_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52955.1"
                     /translation="MKTILTAVILLTAAAVSAETAPLPVSPATNSIASTNAPESYKPR
                     RAASKPDAYTGATPKGKKQKQQTP"
     gene            complement(6063..7409)
                     /gene="zupT"
                     /locus_tag="PKI68_04360"
     CDS             complement(6063..7409)
                     /gene="zupT"
                     /locus_tag="PKI68_04360"
                     /inference="COORDINATES: protein motif:HMM:NF003243.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HNX52956.1"
                     /translation="MPLLSILLIALGLAMDAFAVSITSGITIKNLKVRHALLVGATFG
                     IFQALMPVLGWAVGQWAYRFIAAVDYWVAFGLLLFVGGHMILQAMQPDEEDAEPKNPL
                     HLPTLLTLAVATSIDAFAIGISLSMLRVSILQPVLVIGLVTFALSFAGVYFGRLFGHL
                     NEKKMEVFGGLVLIGLGVKMLVERLIENHAIFSDSRTVWFAFGLTLAAGMATGIGSLL
                     ALFARHTGVRRVSFFLGVSTGLLLWTAFRQMLPCAESDSGRPLTSIAVFFGGFLLTAL
                     IDRLVPTFGNPHEPHRIEDLQEQPAFRRTGMPAALAMAVHSFPEGLAIFVAALHAPAP
                     VAVGAAVALALHNIPEGVATALPMYHATGSRAKACGFSSLTGLAEPLGALIAYTLFYR
                     FMSGLALDFLTAAAAGIMVFIALDGLLPAARAYGKYHHAVYGVVAGMLAAALLALL"
     gene            7593..8348
                     /locus_tag="PKI68_04365"
     CDS             7593..8348
                     /locus_tag="PKI68_04365"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNX52957.1"
                     /translation="MKKAIFKEKIEEIQSELDGLNPLQILQWVEKTVDQPVFASSLGQ
                     EDQVILHLIASNRLAVPVITLDTGRLFPEAYDLIAENESRFGVKIRLLFPDAGEVEAM
                     VEEEGVNLFLKSVDLRKRCCQVRKINPLRRVLKESGGWVCGLRQEQSQTRAELKVVEW
                     DEANGIPKINPLYNWSLEQVAGYLKENRVPYNPLHDRGFVSIGCACCTRAVRPGEDIR
                     AGRWWWEAPEHKECGLHLVNGKFVRSQDAGGVS"
     gene            8345..9253
                     /gene="cysD"
                     /locus_tag="PKI68_04370"
     CDS             8345..9253
                     /gene="cysD"
                     /locus_tag="PKI68_04370"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNX52958.1"
                     /translation="MSRYQLSQLKQLEAESIRIIRDAYSQFENPVMLYSIGKDSGVMV
                     RLAQKAFYPGRVPFPLLHVDSTFKFREMITFRDQFCRDNGLDLLVHTNEEGRAAGASP
                     FTLGSRKYTDVMKTQALLQALDKYKFDCAFGGARRDEEKSRAKERIFSFRDRHHQWDP
                     KNQRPELWSNFNARVNPGESVRVFPLSNWTELDIWQYIRLEKIPLVPLYLAAKRPVVE
                     RDGVLIMVDDERFPLEPGEKPQERLVRFRTLGCYPLTGAVESDADTVDKIVEEMMTTR
                     LSERSTRIIDKDGDSSMEEKKKEGYF"
     gene            9274..11067
                     /locus_tag="PKI68_04375"
     CDS             9274..11067
                     /locus_tag="PKI68_04375"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNX52959.1"
                     /translation="MDATVFAELTWQAWLTLGLVGSIFAVLVFTQFPADFVFLGALTV
                     LFVSGVLTPHEALCGFSSEGMITIGVLYVVVAGLQATGGLNWVTRHVFGIPKNDISAR
                     SRLMWPVAAISAFLNNTPVVAMFLPVVHQWTKNSKIQPSQVMIPLSYAAVLGGICTLI
                     GTSTNLVVNDLYKDHFGSQGLSMFEISKLGIPCAIAGVLFMIFAGRKLLPNRGGADKA
                     FDNPRQYTLEVLVDKGSPLIGKTMEAAGLDSFSSGCLAELIRDSRVISVVQLDEPLRA
                     GDRLIFVGGTDLVGELRTVSGLTIADDQLFKLEAPKHERCLVEAVVSNTCPLVGKTLS
                     EGAFRSVYNAVVLAVARSGERLTGKIADIKLRPGDTLLVESHAGFIPRQRDSRDFYLI
                     SAVEGSTPVRTEKGLLALVILLSMVVVAALNWLPMLEAAALAAAAMLLTGCCTFGEAR
                     KSIEWNVLIVIAAALGIGQALDKTGAAATLATAMLSVSGTNPWIALASLYIGATILTE
                     LITNNAVAALVFPIAMSTAERLDVNPMPFVICLMIAASSSFATPIGYQTNMMVYGPGG
                     YRFTDYFKAGIPLTLVVGAMTILLTPLIWPF"
     gene            11092..>12789
                     /gene="cysN"
                     /locus_tag="PKI68_04380"
     CDS             11092..>12789
                     /gene="cysN"
                     /locus_tag="PKI68_04380"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF004035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HNX52960.1"
                     /translation="MNIERFLSEHENKSLLRFLTCGSVDDGKSTLIGRLLYDSKLLFD
                     DQLSALKRDSVREGNAGEGEIDYALLLDGLKAEREQGITIDVAYRYFTTPHRKFIIAD
                     TPGHEQYTRNMATGASSADLAIILIDARKGVITQTRRHSFIVSLLGIRHVVVAVNKMD
                     LVDFSQARFEEIRADFEALSKELAIPDIRFVPLSALKGDNVVDPSPRIPWYSGPSLLN
                     ILETVDISSDRNLGDFRFPVQFVSRPNLDFRGFAGSVVSGVIRKGDRIKALPSLKEST
                     IKRIVTADGDLAEAFAPQAVTLELNDEIDISVGEMIVHAENIPHVATHFEAMILWLSE
                     TPMNRNTTYLLRHTSHTTKARITGLEYSVDVNTMEKKPAGQLQLNGIGRANITTHQPL
                     FFDAYRDSRETGSFILIDPISNNTVAAGMIERPQAEGERAPLEAQVDRREFLWERGLV
                     SPEARIGRNQHRGKTVLFVGPASGGKQDLARRLELKLFDKGLQAYYLGVPSLLEGLDA
                     DIGQNFRDRDEHIRRIGELARIMTDAGLIFISSLERADEYDLQRLKILNSPNELFVVS
                     "
BASE COUNT         2497 a         3466 c         4189 g         2637 t
ORIGIN      
        1 aaggcggaga cgacggcgaa gccgtcgagt accggcaccg gcaggatatt aaacacaaag
       61 agcatgccgt tgacggcgcc gccgatgagc aggaactttt cggcgctgga cgagccagcg
      121 atttcggcga tgaccatcag taaggaaaaa acaaagcaga gcagcaggtt catcagcggg
      181 ccggagagtg caatcagcgc ccgtttcaac gcgctgcgca cggagaccgg attgaccgga
      241 accgcgcccc aggcgaggcc gaagagcgcg aggacgatga gcgattgcag gcccatctgc
      301 accagcgggt tcagcgtcag gtgtccgttg cgcgcggcgg tgtcgtcgcc gaagcgcagg
      361 gcggtgtgag cgtgtgcgta ttcgtggcaa cagatggaaa acaccacgaa ggtgatccag
      421 ccgaagtaga gaaacgggtc ggtcagggcc tgttgaatga acattgcgga ttattccttt
      481 tccgggacgg gtgtgagcag cagggtgtga atgcggcgcg gcgtggcgtt ctggacggtg
      541 atttcaaggc cgggggcggc gagctttgtt ccggcgggcg gaatggtgcc gagttcgctg
      601 aggacgtagc cgccgatgga gtcgtattct tcgctttccg gtatgtgcag gccgagttct
      661 tcgttgaggt cggcgacggc catgccggct tttaccagcg cggtgccgtc ggaacatttc
      721 aggatgtcgc gttccgcgag atcgtattcg tcgcggattt cgccgatgag ttcttcgatg
      781 acatcttcga tgcagacgat gccggcggtt ccgccgtatt cgtcaacgac gagcgccatg
      841 tggaagtgct gcgagcgcat ctggcgcagc aggtcggtga tgggcatggt ttcgggaacg
      901 gcattgagtt ttttgaccag ttccgccacc ggatgggttt cttcgccgcg cgcaaagagt
      961 cgcagcaggt ctttgacgtg gacgactccg gcgacgttgt cgatggtttc ttcgtaaaca
     1021 ggaaagcggg aatgggccga tttctgaact ttttcaacgc attcggcgac ggtgctgccg
     1081 gcgcgcagcc cttcgatatc gacgcgcggc gtcatgattt cgcggacgac ggtgtcgccg
     1141 aactcaaaga cgctgcggat gatgtcgcgc tcttcctttt ccagctcttc gtcgtcggcc
     1201 tgatcgacca gcgtcatgat ttcctcttcc ggcgtggggc tgtcgtcttc atcggcggag
     1261 gagagcgcgc gggcgaggag tccgcgttcc agcatgtaga ccggccagac cagcggaaag
     1321 agaaggcggg tcaggccgat gacgacgggc aggaagaaga gcgacagccg gtcggcgtag
     1381 ctttcggcca tgacgtaggg gatgacgcgg atgaagagca gatagagcgg cgtcatgacc
     1441 agcatgaggc cggcccactc ggcaatcagc gggaagttaa aagcgtggaa ggcgaaatag
     1501 atggcggaga tttcgaagaa tgtcaggccg aagcgcagcg tggtgcgcaa caggttccat
     1561 cgtccggccc agctttccag cgcggctctg gcctgcggtt ttttttctgc cagccggagc
     1621 accccggcat tgccggtttc ccggaaggcg gcaaacagta cggcccagaa gccggcaatt
     1681 aacagcgccg caaggctgcc ggcgagatga ttgaagtcca ctccgttcat gtcagtccgc
     1741 gatcagattt ttgagttcgg tctgcaatgc cgaaagccgg cgcatctccg tgcggcgcat
     1801 ggcggcgcgt ttggcgggcg tattgtcgtc ggcgccggaa aggtggttga agccgtgggc
     1861 gatgtagagc gcaagttcgg cgtccgcacc gccgtggcga gggccttcct gtaccgcccg
     1921 ttcaacgttg acgatgaggt cgccggtcgc gccgtcttcg ccgggaaccg gttcgccgcg
     1981 gaagctgatg acatccgtcg cccggtcttt gccgaagaat tcgcggttgt actgcgtaat
     2041 gccggcgtcg tccgtcagca gcagggtgac ttcctgccag acggtttccg gatcggcagc
     2101 ctcaagcgcg gcggccagtt cagcggtcag ccggcggatg gccggcaggt tgatccggcg
     2161 tttctgccgg tttatcagtg cggttttcat ttttagggta agccacgcgg ccgtgaagca
     2221 tgttggtgag ggagaagata aaggactgtt tgattttatt gagttcggcg agcgtgaggt
     2281 cgcattcgtc cagctggccg tcgcgcagac gggcgtcgaa aagatcgttg atgagattgg
     2341 tgatgcgtac cggcgtcggc ttgccgagcg agcgcgaggc cgcttcacag ctgtcggcga
     2401 gcatcaggat ggccatttcg cggttctggg gccgcgggcc ttcgtagcgg aaatcttcgt
     2461 cgttgatcgc gagctgcccg gcggcgtttt ctttctgatg gcgggttttg gctcgatggt
     2521 agaaaacgga aaccaggctg gttccctgat gctgttcaat cccgtcgatg atcacctgcg
     2581 gcagtttgaa ttttttcgcc agcgtgacgc cttctttgat gtgcgagaca atcacgagcg
     2641 tactcatgct cggggtcagc tcatcgtgcg ggttttcggt gaactgaata ttttcgctga
     2701 agaagccggg cttaacaagt ttgccgatgt cgtggaaata ggcgcagacg cgcagcatca
     2761 gcccgttggc gccgatggct tcggcggcgt tctgtgcaag gctggccagc atcagcgaat
     2821 ggtggtaggt gccgggcgcg ttgatcgcca gactctggag cagcgggtgg ctcaggtctg
     2881 aaagttcgag cagtgaaatg tcggtggtga ttttaaagag gtgttcgaag accggaatca
     2941 gcagcatggc gagcagcgcg ctgatcagtc cgcacagcat ggccgcaccg agctgcggca
     3001 gaagcacctg ccacgatgga ttcttgagca ccgccatcac cagcgcaaag aggatttcga
     3061 cagcgccgat ccagaatccc gcgcggaaca gcgccgtgcg gcgtttgaca ttccgcgtgg
     3121 tgtagacggc tgccgccgac gccagcagtc cgagtacaaa aagatcaaaa cttcccccga
     3181 gcagagtggc cagtgcgaag ctgacccaga acccgaccat cagtcccggc gcgctgccga
     3241 gcagaatggc ggccagcagc ggcgcgatcg acagcggcat gatgaagtcg agtacggccg
     3301 gcggcatcag gcgcaggttg acggtggcgt agagcatgcc ttttccgatg atcagcggca
     3361 gaatcgaaag tgttatcagc agcagaactt tacggcggct gtgcagcagt gcaggctgta
     3421 cgatgccggc cacggctacg gtcagggcaa gtccgagcag cagcagcagg ccgctgcctg
     3481 ccagccgctg gatgcgttcc gccggcgttt cgagctcgct cagccgtctt tcatgagcgg
     3541 tcagccattt caaggtctgt tcgctcacag tgtcgccgtt gcggaccaga atggtgccgg
     3601 cgcttacggg ttcaatcacc ggatcaacca gaccggttgc ctgtgtgcgg cgcgatgttg
     3661 tttcggcggc atcataaacc agattgggcc ggagccacgg cgcaaccagt gcgcggatag
     3721 cttcttcgtt gcgggttccc cggggaagat tctgcgcaat tgccgtgacg gttgctttaa
     3781 tcgcgtcggg ttgcagcgca aaaccgtcca gcgtgcggat aacgtcggtt ccgttcccgc
     3841 gcagtaccac ttttccggaa ggtgccgcgc cgttaaaccg ggtttgtgca tcgccggctg
     3901 aaataatgcc gcgcgacgca gtgttggaaa gcgccgtgaa aaaaacggtc cgtacatcaa
     3961 tctgcttatt ttccggaatg atttccgtca gctgattcgt cgtcaggatc agccgggcat
     4021 cttcaatggc ggaggtgtcg atggaaaaga ccggcagaac ggcctcggcg gcggcgcgct
     4081 gtccggcctg tgtggcggcg agattttcgg cgaggaaatc aatgtcggcc gtaatggtga
     4141 ccggcgactg ctgcccggct gtcagcggct gagggcggag caggcggccg ctgtagaaaa
     4201 acaccaccag cgcaacccag aggacaaccg ccagcagcag gttgaagagc gggaagtgcc
     4261 gcgccgcttt acgggcggtg acgtctctgg tcgctgcaac acggtttatt ttttttctga
     4321 agagtttccg tttcaaatcg ggcctttgct gatgaagggt tataccctgt ggtgacggta
     4381 tgcgcgaatg attttctgca ccagcgggtg gcgcaccaca tcgtcctcgt cgagttcgat
     4441 gaacgtgaga ccgtcgattt tactgagaat gtggcgggct tcggtcagac cggatttctt
     4501 tttatcgggc aggtcgacct gggtcgggtc gccggtgatg accgcgcgcg attcaaagcc
     4561 gaggcgcgtc agcagcatca gcatctgcag cggcgtggtg ttctgcgctt cgtcgagaat
     4621 gacgaacgca tggttcaggg tgcggccgcg catataggcc agcggcgcga cttcgataat
     4681 accgcgttcc atcagccgct gaatctcttc ggccggaagc atatcgtgaa gggcgtcgta
     4741 aagcgggcgc aggtacggat cgactttttg ctgcaggtcg ccgggcagga agccgagcgc
     4801 ctcgccggct tcaatggccg ggcgggttag aatgatgcgg ctgacttcgt ctttaagaag
     4861 tgcggagaca gcggcggcca tggccagata ggttttgcct gtaccggccg ggccgagtcc
     4921 gaacgtgatc gggttgtgtt tgatggcatc cagataaatg tactgcccga aggtgcgcgg
     4981 aaaaaccggc gatttgcgcg ggctgacgtc gatccgcatg gcagagaagg tctccagcat
     5041 attctcgcgg ccttcggtga acgatttcag cgcatagtgt acgccgccgg tattcagcga
     5101 aaacccgcgt tcccgcgccg cgcgcagcgc ttcgaaaaac cgcacggcgg attctgttcc
     5161 gcggccttcg attttcagcc aggtatcgcg cgccgtcagg gtgacatcga acgcgcgctc
     5221 gatggcttcg agcgttccgg cgggatgggc ggcgatatcc tgcgcctcgc gggcgttgtc
     5281 gaaatggaga gtgatttcac tcatgtaggc cggaaaggta tgctttttgc ctgcacaggg
     5341 tcaatttgaa aggccggagc ggctcagcgg acggcaaggc ggtttccgtc atattccacc
     5401 gtcataccgg ggcggacttt gcggcgcagg cgcagttcgg tttcgccatc gacggtgacc
     5461 agcccttcgc tgatgacgat ccgggcttcg ccgccggtgc cgcacaggtg tgcggctttc
     5521 agcagtttat ccagttcaat atattcgccg gtgatagtga actcagccat gttttttcct
     5581 ctgcgcacgg acgagttcca gttcctcgtg agtggcgcgg cggcggttgt agagcgcggc
     5641 gactttgtcc tcgagcgcct gccagtcttc gggggatgcc ggggtgtcgt aatcccagcg
     5701 ctcttcgtcg gagcgcttga actgccattt gatgcccttg ttcatcgtaa tcgcaatacg
     5761 cacggtgcgc ttcacgccgt cttcgatttt ttcaatccat tcaatgtcgc gcttcatggg
     5821 gtctgctgtt tctgcttttt accttttggg gtggcaccgg tgtaagcatc cggtttggag
     5881 gcggcccggc ggggtttata actttcaggc gcgttggtgc tggcgatgct gtttgtcgcc
     5941 gggctgacgg gcagcggggc ggtttcagcg gaaaccgcgg cggcggtcag cagaattact
     6001 gcggtcagga tggttttcat gcgaggtgta tacactttgc ggtatcgcgc ggaaaggtaa
     6061 aatcagagca gagcgagcag ggcggccgcc agcatgccgg ccacaacgcc ataaacggca
     6121 tggtgatatt tgccgtaggc gcgcgccgcc ggcagcagtc cgtccagcgc aatgaagacc
     6181 atgatgcccg ccgccgcggc ggtgaggaag tcgagcgcca gtccgctcat gaagcggtaa
     6241 aacagtgtat aggcaatcag cgcgccgagc ggttcggcca gtccggtgag cgatgaaaaa
     6301 ccgcaggctt tggcgcggct gccggtggcg tgatacatcg gcagcgccgt ggcgacgcct
     6361 tccggaatgt tatgcagcgc cagcgccacc gccgcgccga ccgcgaccgg cgcgggggcg
     6421 tgcagggccg cgacgaagat ggccaggcct tcgggaaaac tgtgcaccgc catcgccagc
     6481 gcggcgggca tgccggtgcg gcggaaggcc ggttgttcct gtaaatcttc gatccggtgc
     6541 ggctcgtgcg ggttgccgaa cgtcggcacc agtcggtcta tcagtgccgt cagcaggaag
     6601 ccgccgaaga acaccgcgat ggaggtcagc ggccggcccg aatcgctttc ggcgcacggc
     6661 agcatctgcc ggaaggcggt ccacagcagc agcccggttg aaacgccgag aaaaaaggag
     6721 acgcgccgca caccggtgtg ccgcgcgaaa agcgccagca ggctgccgat accggtcgcc
     6781 attcccgcgg ccagtgtcag gccgaaagcg aaccagactg ttcgcgaatc ggaaaatatc
     6841 gcgtggtttt caatcagacg ctccaccagc attttgacgc ccagcccgat caataccagg
     6901 ccgccgaaaa cctccatttt cttttcattc aggtggccga aaagccggcc gaaatagact
     6961 ccggcaaacg aaagcgcgaa cgtcacgagc ccgatgacca gcaccggctg caggatcgaa
     7021 acgcgcagca tcgaaaggct gataccgatg gcgaaagcgt caatgctggt ggcgacggcg
     7081 agcgtcagca gcgtcggcag gtgcagcgga tttttcggct ccgcatcctc ctcgtcgggc
     7141 tgcatcgcct gcaggatcat gtggccgccg acgaagagca gcaggccgaa tgccacccag
     7201 tagtccacgg ccgcgataaa ccggtacgcc cattggccga ccgcccagcc gagcaccggc
     7261 atcagggcct ggaaaatccc gaaggttgcg ccgacgagca gggcgtggcg caccttgaga
     7321 tttttaatgg tgattccgct ggtgatggaa accgcgaagg catccatcgc cagaccaaga
     7381 gcgatcagga gaatcgaaag taagggcatg ggctgaattc cggtttagtt tcactgaatc
     7441 tatataaaac cagcggtttg tagaggcttt tttcaagcgc cgcgcatttt ctgtaaaaca
     7501 tgatcatctt atatgtatat ttttcgtgtt taaactttac ttaggtgata aacatactca
     7561 tattaaaaaa gttaagttaa aggagcagtg cggtgaaaaa ggcgattttt aaggaaaaaa
     7621 ttgaggaaat tcagtcggaa ttggacgggt tgaatccctt gcaaattttg cagtgggttg
     7681 aaaaaaccgt cgaccagccg gtttttgcca gttcgctggg gcaggaagat caggtgattt
     7741 tgcatctgat tgcgtccaat cggctggcgg ttccggtgat tacgctggat accggccgcc
     7801 tgtttccgga ggcctacgat ctgattgccg agaatgagtc gcgctttgga gtcaaaatcc
     7861 ggttgctgtt tccggatgcc ggggaggtcg aggcgatggt ggaagaggaa ggtgtcaacc
     7921 tgttccttaa gagcgtcgat ctgcggaagc gctgctgtca ggtgcggaag attaatccgt
     7981 tgcgccgggt tttgaaggag tccggcggat gggtttgcgg gctgcggcag gagcagtcgc
     8041 aaacgcgggc ggagctgaag gtggtggagt gggatgaagc caacgggatt cccaagatta
     8101 atcctctgta taactggtcg ctggagcagg tggccggtta tctgaaggaa aaccgggttc
     8161 cgtataatcc gctgcatgac cgggggtttg tcagtatcgg ctgtgcctgc tgtacgcggg
     8221 cggtgcggcc gggggaggat atccgggccg gacgctggtg gtgggaggcc ccggagcaca
     8281 aggagtgcgg gcttcatttg gtgaatggaa agtttgtgcg aagtcaggat gcaggaggag
     8341 tgtcgtgagc agatatcagt tgagtcagct gaagcagttg gaggcggaga gtatccggat
     8401 tatccgtgat gcgtacagtc agtttgagaa tccggtgatg ctgtattcga tcggcaagga
     8461 ttccggcgtg atggtgcggc tggcgcaaaa ggcgttttat ccgggccggg ttccgttccc
     8521 gctgctgcat gtggattcga cgtttaagtt ccgcgagatg attacgtttc gcgatcagtt
     8581 ctgccgcgat aacgggctgg atctgctggt gcatacgaac gaggaggggc gcgcggccgg
     8641 ggcgtcgccg tttacgctgg gaagccgcaa gtacacggat gtgatgaaga cgcaggcgct
     8701 gttgcaggcg ctggacaagt ataagtttga ttgcgccttc ggcggcgcgc ggcgggatga
     8761 ggagaagtcg cgggccaaag agcggatttt ttcgttccgc gaccgccatc atcagtggga
     8821 tccgaaaaat cagcggccgg aattgtggtc gaattttaat gcgcgggtga atccggggga
     8881 gagtgtgcgg gtgtttccgc tctcgaactg gacggagctc gatatctggc agtacatccg
     8941 gctcgaaaaa attccgctgg tgccgctcta cctggcggcc aagcggccgg tggtcgagcg
     9001 cgacggtgtg ctgatcatgg tggatgacga gcggtttccg ctggagccgg gcgaaaagcc
     9061 gcaggagcgg ctggtgcgtt tccgcacgct gggctgctat ccgctgaccg gagcggttga
     9121 gtcggacgcc gacacggtcg ataagatcgt cgaagagatg atgaccacgc ggttgagcga
     9181 gcgctcgacc cgcattatcg acaaggacgg cgactcctcc atggaggaga agaagaagga
     9241 aggctacttc taacagaatt ttgagagttc attatggatg cgacagtttt tgcagagtta
     9301 acgtggcagg cctggctgac gctgggtctg gtcgggagta ttttcgccgt gctggtgttt
     9361 acgcagtttc cggcggattt cgtttttctg ggcgcgctga cggttttatt cgtcagcggc
     9421 gtgctgactc cgcatgaggc gctgtgcgga ttttcctctg agggaatgat cacgatcgga
     9481 gtgttgtatg tcgtggtcgc cggactgcag gcgaccggcg gattaaattg ggtgacccgc
     9541 catgttttcg gtattccgaa gaatgacatc tccgcccgca gccgcctgat gtggccggtt
     9601 gccgcgatca gcgcgtttct caacaacacg ccggtggtgg ccatgtttct tcccgtggtt
     9661 caccagtgga ctaaaaacag caagatccag ccgtcgcagg tgatgattcc cttgagttat
     9721 gcggcggtgc tgggtggcat ctgcacgctg ataggaacca gtaccaatct cgtggtgaat
     9781 gatttgtaca aagatcattt tggctcccaa gggctgtcca tgtttgagat ttccaagctg
     9841 ggaataccct gcgcgattgc cggcgtgctg tttatgattt ttgccggacg caagctgctg
     9901 ccgaaccggg gcggagccga caaggcgttt gataatcccc gccagtacac gttggaagtt
     9961 ctggtggaca aggggagtcc cctgatcggt aaaaccatgg aagccgccgg gctggacagt
    10021 ttttccagcg gttgcctggc tgagctgatt cgcgacagcc gcgttatttc tgtcgtacag
    10081 ctcgacgagc cgttgcgggc aggcgaccgg ctgatctttg tcggcggcac cgatctggtc
    10141 ggagaactcc ggaccgtttc cgggctgacc attgcggacg atcagctgtt taagctggag
    10201 gccccaaaac atgagcggtg cctggtggag gcggtggttt ccaacacctg tccgctggtc
    10261 ggtaagacgc tgagtgaagg ggcgttccgc agcgtgtata acgctgtggt tctggctgtt
    10321 gcccgcagcg gagagcggct gactggaaaa atcgccgata tcaagcttcg tcccggcgat
    10381 accttgctgg tggaatccca cgccggcttt atcccgcgcc agcgcgactc ccgcgacttt
    10441 tacctgatca gcgccgtgga aggttcgacg ccggtgcgga ccgaaaaggg gctgctggcg
    10501 ttagtgattc tgctgtccat ggttgttgtg gctgcgctga actggttgcc gatgcttgaa
    10561 gccgccgcgc tggcggcggc ggccatgctg ctgaccggtt gctgcacctt tggagaagcc
    10621 cgtaaaagca tcgaatggaa tgtactgatt gtcatcgcgg cggcgctggg aatcggtcag
    10681 gcgctggata aaaccggcgc ggcggccacg ctggcgacgg cgatgctttc ggtgtcagga
    10741 acgaatccgt ggatcgcgct ggcttcgctc tatatcggtg cgacgatttt aaccgagctg
    10801 atcaccaata atgcggtggc ggcgctggtg ttcccgatag ccatgagcac ggcggaacgg
    10861 ctggacgtta atcccatgcc ttttgtgatc tgcctcatga tcgcggcgtc ctccagcttc
    10921 gccacgccga ttggatatca gaccaacatg atggtgtacg gccccggcgg ataccgcttc
    10981 accgattatt tcaaggcggg aattccgctt acgctggtgg tcggggcgat gaccatcctg
    11041 cttacaccgc tgatctggcc gttttagaaa aatcagtttt ggagaatcga tatgaatatt
    11101 gaacgttttt tgagtgagca tgaaaacaag agtctgctgc ggtttctgac ctgcggctcg
    11161 gtggacgacg gcaagtccac gctgatcggg cggctgctct atgacagcaa gctgctgttc
    11221 gacgaccagc tcagcgcgct gaaacgcgac agcgtgcgcg aaggcaacgc cggcgaaggc
    11281 gagatcgact atgcgctgct gctggacggc ctgaaggccg aacgtgagca gggcatcacg
    11341 attgacgtgg cctaccgcta cttcaccacg ccgcaccgca aattcatcat tgccgacacg
    11401 cccggccacg agcagtacac gcgcaacatg gccaccggcg cgtcgtcggc cgatctggcc
    11461 atcattctga ttgatgcgcg caaaggcgtc atcacgcaga cccgccgcca cagcttcatc
    11521 gtgtcgctgc tgggcatccg ccacgtcgtg gtcgcggtca acaaaatgga tttggtggat
    11581 ttcagccagg cgcgctttga agaaatccgc gccgactttg aagcgctcag caaagagctg
    11641 gcgattccgg acatccggtt cgttccgctc tcggcgctca aaggcgataa cgtcgtcgat
    11701 ccctcgccgc ggattccgtg gtacagcggg ccgtcgctgc tgaacatcct ggaaaccgtc
    11761 gatatttcgt ccgaccgcaa tctcggcgac ttccgctttc cggtccagtt tgtcagccgc
    11821 ccgaacctcg atttccgcgg gttcgcgggc agcgtggtct cgggggtgat tcgcaaaggc
    11881 gaccgcatca aagcgctgcc gtcgctcaaa gaatccacca tcaaacggat tgtcaccgcc
    11941 gatggcgacc tcgccgaagc gttcgcgccg caggccgtca cgctcgaact caacgacgaa
    12001 atcgacatca gcgtcggcga gatgatcgtg cacgccgaaa acattccgca tgtggcgacg
    12061 cacttcgagg cgatgatcct gtggctctcg gaaacgccga tgaaccgcaa caccacctat
    12121 ctgctgcgtc acacgtcgca caccaccaag gcgcgcatca ccgggctgga gtacagcgtc
    12181 gacgtcaaca ccatggagaa aaagcccgcc gggcagttgc agctcaacgg catcggccgg
    12241 gccaacatca ccacgcacca gccgctcttt ttcgacgcct accgcgacag ccgcgaaacc
    12301 ggcagcttca ttctgatcga tccgatcagc aacaacaccg ttgccgccgg aatgatcgaa
    12361 cggccgcagg cggagggcga gcgcgctccg ctggaagcgc aggtcgaccg gcgcgaattt
    12421 ctgtgggaac gcggactggt atcgccggaa gcgcgcatcg gccgcaatca gcaccgcggc
    12481 aaaaccgtgc tgtttgtcgg cccggcatcc ggcgggaagc aggacctggc ccggcgtttg
    12541 gagctcaagc tcttcgacaa agggttgcag gcctactacc tcggcgtccc gagcctgctg
    12601 gaggggttgg atgcggacat cgggcagaac ttccgcgacc gcgacgagca catccgccgc
    12661 atcggcgagc tggcgcgcat catgaccgat gccggtctga ttttcatcag ctcgctcgag
    12721 cgcgccgatg aatacgacct gcagcgcctg aagatcttga attcgccgaa cgagctgttt
    12781 gtcgtcagc
//
LOCUS       DAOPZO010000045        12775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_5437_length_12775_cov_9.240409, whole genome shotgun sequence.
ACCESSION   DAOPZO010000045 DAOPZO010000000
VERSION     DAOPZO010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 12775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12775
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5437_length_12775_cov_9.240409"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1649)
                     /locus_tag="PKI68_04385"
     CDS             complement(<1..1649)
                     /locus_tag="PKI68_04385"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX52961.1"
                     /translation="MKLNSKLLLWAVLVAGVTLVCFSPVLRGEFLYWDDDGLFVRNPY
                     YRGLSPVHWQWMCSTFFFGHWQPLSWLSCALDYKAWGMNPHGWHVTSLLLHVVNSMLV
                     YLLCLPFLKERTGRYAAAALAALFWAIHPLRVETAAWLATRGYLLCTTFCLLTVLFYL
                     RAVKQKRYPLAALLCFTLATCTKGIGMMLPAVLLLIDWAPFRRITSVRTALSCVIEKT
                     PFFGLSLLTGITAFLAKKSDGGMMPVEHYGLIERFGQAVYGVWFYLCKTVFPFNLAPL
                     YDKRPEPGPVMVSLALTAAAAMVLFLLRRRIRPVLITSGAFLLLIFPMLGFTQSGTQL
                     FADRFTYLAAVPVSILLAAGLVLPVPMRRLICGALAALLVLFGVQTAIWAVSWNSNLT
                     LWWRAVSVDGSNAKACNSAGLALMDCQQYEKALECFEKSLQLNPSVLALHNRALALAM
                     MGRFDEAFPDWEIALSVPGTPSELRAKMLWIRGWVFEQTGNLKAAENDYSSVADDCTV
                     DPVQRVDTLQLRAALYERTGQKEKALTDLETVLKLPDPFGN"
     gene            1759..2097
                     /locus_tag="PKI68_04390"
     CDS             1759..2097
                     /locus_tag="PKI68_04390"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNX52962.1"
                     /translation="MKTKTGKSKASKGFTLVEILIVVAIIGLIAAIGIPSFINSRQNA
                     ETNMKAINVAAVNAAKDQWAIINNKATGTTVAWTNIAPYIGNSVSNQAGLTVGGSAIT
                     INPVGTSASY"
     gene            complement(2098..3897)
                     /locus_tag="PKI68_04395"
     CDS             complement(2098..3897)
                     /locus_tag="PKI68_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX52963.1"
                     /translation="MFSLCWIESREANLTGLLNNSFFGGVERTILSSFPLMYNDVALH
                     YIALWTFVFGAALVWAVMHLRRNARIRQTARDCEMFRLRVRNILQHSREFLFGFDLRG
                     GRFDYLSPACLSLTGYTAEELKALEPQELLQHLHPEDREKIDRLIAQLRQRNKEREWL
                     GLVEYRFLHRDGQYRFFSDHLHVVYDESGRAVYLSGSVRDVTQIARMEDSMRILEKKF
                     QDGQKMAGLGLLASGIAHDFNNLMTVVLGNAELALLECGGSDGGVLDEIKKTSLRAAE
                     LANQMLVYTGKASLVVSSINLSSVVKEMGSLLDVSISKKVSIQYCLDENVPLIRGDIS
                     QIRQVAMNLITNASEAIGDRSGVIAISIHEVELKAGELERTFPQGGLPSGRYIRLEVS
                     DTGDGMNEETLQRIFDPMFTTKITGRGLGLASVLNVVQRHNGAIEVRSQVGHGTVFRI
                     YFPVEAQDGDLRAEELQAEGGEVWRGYGTALVADDEEAIRNITAALLERFGFRVITAS
                     DGFETVDLYTENAGDITLLLMDLNMPRLNGIEAALRIRHINPKMPVLFMSGYPREQVM
                     ERFGQHAHTDFIQKPFQSGELMAGVRSVMETRR"
     gene            complement(3842..4471)
                     /locus_tag="PKI68_04400"
     CDS             complement(3842..4471)
                     /locus_tag="PKI68_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX52964.1"
                     /translation="MRILIAEDEKVTRMRLEKTLSGWGFEVEAVADGQAAWERLCSDD
                     PPRLCIIDWLMPRMEGPELCRKVREKFPEEYFYLIILSAQQGVDNLIEGLSAGADDYV
                     TKPFLGRELRSRIDVGVRVIGLERMLANKVHQLEAALEDVKQLRGLLPICSYCNKIRN
                     DEDYWEQVDSYITRRSNVEFSHSVCPSCYEKHVQPMLDRKQGGESDRTA"
     gene            complement(4537..6294)
                     /gene="dnaG"
                     /locus_tag="PKI68_04405"
     CDS             complement(4537..6294)
                     /gene="dnaG"
                     /locus_tag="PKI68_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNX52965.1"
                     /translation="MKQIPRETIEEVRTRNDIADVIGSYLSIKNAGTRFKALCPFHKE
                     KSPSFTISPDRQIYHCFGCGAGGDVIRFVQEYEKVDFMTALQMLADRAGMELSLEEGG
                     EGSGADKRKLFQIHEGVAQLYHRILLEHESGEAGRAYLSTRSLKPETVKEFQIGFAPD
                     RFDALEKWALQKKIPLELMEQAGLMIKSDKRSGYYDRFRNRLMFPIRDEAGRVIGFSG
                     RAIVKDEKSGKYVNSPETPLFHKSRVLFAIDKARRAMADSRTAIVCEGQLDAIRCHEA
                     GIANVVASQGTALTADHARMIRRYADEVILVLDADAAGQKAALKSSAAFIAEELSVRV
                     ASLPEGEDPDSLVRDHGAEALRSRIAGAVSALDFLIDVMAAHENLKTEAGVMRTARAV
                     QALIAQAPGAVQRDRMVTHAAGRLGLSPAAFRLDLARKKEHPAPVAAPEVPVAPVKHP
                     DEEMALIQMLFLHPAEVMPVVADHLPPAHLTDPDCRLLLELMLEDPAALMDSIPDDRP
                     EAQRLAARIQVEEMKLRGDVPPAKAAQDIVMALWRQALTRRRRELQAAGRAAEATELT
                     QQLYRLKQDWVHAVDFLVV"
     gene            complement(6298..6843)
                     /locus_tag="PKI68_04410"
     CDS             complement(6298..6843)
                     /locus_tag="PKI68_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNX52966.1"
                     /translation="MWFPEMLYTIDILFVLFVLFFAVRGVWNGLSGELANVVTLLALL
                     TGFCFFYPQLENLAKDHWNLPAAALRFIVPAVLILASVLLFVLLRILFRQMLKDLADT
                     NADKVGGVLVGMLRGILLGLTVFVGLSLMPNDALYRVLSEKSAIGGWVCNTLTPWASP
                     RIGELPVLKKDMQNRIDDITR"
     gene            7150..9873
                     /gene="nrdD"
                     /locus_tag="PKI68_04415"
     CDS             7150..9873
                     /gene="nrdD"
                     /locus_tag="PKI68_04415"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HNX52967.1"
                     /translation="MAETITSKDALFEGFRKRSGLIVPFSHKKIEMAIHLAVEEVARK
                     QNTPRNEGLAPKITAQVLEQLNNPQSEFFVRPEADGKRIPHIEDVQDLVEILLAESGE
                     TLVVAAYKRYRKQREIARNSIRVRDGSIKKDKVDVTDASLLLVESHSSEVTLPWDRKR
                     IVKQILDKTDLAVDTAISIAKAVENRIIASNMKAVNTTLIREMVNNELAERGFSAQLR
                     DLSLYGVPLDYVEKLMFTKSTENSNIVNNNPEAVNLGIAELVLKQWALDTIFTPDIKR
                     AHNTGAVHLHDLGYPHRVYCSSHSIEYVKKYGLKGLINLNTESSPAKTASVLTGHLNT
                     FLASMQANYAGALGIAYINILYAPYLEGMNARELKQIAQELIFNGSQNAFSRGGQTLF
                     LDFNIHSGVPGYMKTVPAIGPGGKYMLRLKSGLKVRLEEVLRDDRDIGGYRLMDLYLQ
                     EADGSQRLVLREIAGKEGLEYDAAVAADLEARGEKIVTYGDYTKEAQDFCRSLLEVWG
                     DGDSNGRIFEFPKCDFHISDETFVDPEQYQIYQAACQLAAKNGSTYFIFDRDEVTLSA
                     CCRLRTTISDNRMLRHPENMRFCGFQNVTINIPQAAYRAARKGGDILKNFFDEIDATM
                     ELCAQAHLQKKAKIAQMMSGNGHPLWQIGKESNDGKPYVNLDTCTYILGLIGVNDTVK
                     FLIGQELHDSDEAQQFGLRIVAHMYTRTKKLSKKYNLKFTLEESPAESAARRLAKTDL
                     IYFHDEAKDVVKGETEDVCYYTNSIHLTADAPVGLVQRIVEQSKYHSLIESGAITHAF
                     VGEEQPSAASIEKLMTETFFRTQSAQVCISPEFTFCNNCHHQVRGLIETCPACQSADV
                     VGETRVVGYFSKVQNWNKSKRYGELKARQRGQYAIETAEPCTEKKPVSVKG"
     gene            9900..10295
                     /locus_tag="PKI68_04420"
     CDS             9900..10295
                     /locus_tag="PKI68_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52968.1"
                     /translation="MKKPFKIKVFGKEGCAKCKMLNQRLDKLLEEKEWSDFEKEYCDV
                     ETVDGLVAFASAECVNPQRIPALLVTRRQEETERYVPVPTRNPQPRDPVCGKSKLYQY
                     VGLQTDYSDEGKGIISPRMITAVLEEARG"
     gene            10382..11038
                     /locus_tag="PKI68_04425"
     CDS             10382..11038
                     /locus_tag="PKI68_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HNX52969.1"
                     /translation="MIDYPGFLCGVFFISGCNFSCGFCHNAPLMGKKQKGLPWSRLDE
                     LCRKFKTEWVDAVCITGGEPTLAPDLIALIEFFRGYGFKIKLDSNGARPEVLKECLPL
                     VNYVAMDIKAGLSGYPELVGFTQTEKIAESVKLIMSSGLDYEFRTTVIEPFHTDEQMR
                     EIGELIQGARRYCIQPFIPKDNLPDPRLRETKRTTPARLRQIEKLMKPYASEIIVRGA
                     "
     gene            11202..12254
                     /locus_tag="PKI68_04430"
     CDS             11202..12254
                     /locus_tag="PKI68_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52970.1"
                     /translation="MNTMRKMFYSLCVAGLALSASIKAEPVSLPIPGEGWNLSFDAPP
                     IIWTKCDKTSSGVSYLGSSENGVVISFFVEEPDGESRGNKACADYYWPLGKKNPMIDQ
                     STVEIEETKDFVKVSYRLRGKYKGTAIDMPNINLYFEYQGKWVDVHISKLPFEDEDSA
                     RKILDAFVESLKCEMISGRSAPANPEKLVFQTRSFPLPQHGTFTLDIPTSWKQRFQQP
                     KDETLPPTITFSPKQGDEFEVMITPIWSPKNDLSFTKPESVRQLIDSDLQRMLPDAVE
                     KQVPIQEFKGIDGSGYYFFVTDKATKPGEYPYAVRAGISTGDLLLYVTVLCRSKDGGE
                     IEAVIKALETAKQEKK"
     gene            12388..>12775
                     /locus_tag="PKI68_04435"
     CDS             12388..>12775
                     /locus_tag="PKI68_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNX52971.1"
                     /translation="MLSKENAVLIFIDVQGRLAELVDGADALFKNLRRLLEGMKALDV
                     PVIVTEQIPEKLGPTRDEFQPFITAPPVTKTSFSCCGEPAFLKSLKKTGRRHVILCGI
                     ETHVCVYQTAMHLLKSGFKVYAVADAV"
BASE COUNT         3243 a         3817 c         3143 g         2572 t
ORIGIN      
        1 tgattaccaa aaggatccgg cagtttgagc acggtctcca gatcagttaa tgctttctct
       61 ttctgcccgg ttcgctcata aagcgccgcg cgaagctgta atgtatcaac ccgttgcaca
      121 gggtccacgg tacaatcatc tgccacactg gaataatcgt tttcagcagc tttcaggttt
      181 ccggtctgct caaaaaccca tccgcgaatc catagcattt tcgcacgcag ttcgctgggc
      241 gttcccggca cagacaatgc gatttcccag tcaggaaaag cttcgtcaaa acgacccatc
      301 attgccagcg ccagcgcacg gttatgcaaa gctaaaacag acgggttgag ttgcagggac
      361 ttctcaaagc attccagcgc tttttcgtat tgctgacaat ccatcagtgc cagtcctgcg
      421 ctgttacagg cctttgcatt acttccgtcc actgatacgg cacgccacca aagcgtcaga
      481 ttgctgttcc atgaaacagc ccagattgcc gtctgcacac caaacagaac cagcaacgcg
      541 gccagcgctc cgcagatcaa tctgcgcatt ggaaccggga ggacgagccc ggcagccaaa
      601 agaatagaaa caggaacagc cgccaaatac gtaaaccggt cggcaaataa ttgagtgcca
      661 ctctgggtga atccaagcat cggaaaaatc agcagtaaaa aagcgccgga cgtaatgagt
      721 accggacgga tccgccggcg taaaaggaaa agaaccatcg ccgcggccgc tgtcagtgcc
      781 aatgaaacca ttaccggacc gggctcaggc cgtttgtcat acagcggagc aagattgaac
      841 gggaaaactg ttttgcacag ataaaaccag acgccatata cagcctggcc aaaccgttca
      901 atcagcccgt aatgctcaac cggcatcatg ccgccatcag acttcttagc cagaaaagct
      961 gttataccgg tcagcagcga aagaccgaaa aacggagttt tctcaatgac acaggaaagc
     1021 gcggtgcgca ccgatgtgat ccgccggaat ggcgcccagt cgatcagcag caacaccgcc
     1081 ggaagcatca tgccgatgcc tttggtacac gttgcaagtg tgaaacagag cagcgccgcc
     1141 agcggatagc gcttttgttt aacggcccgc agatagaata aaaccgtcaa cagacagaag
     1201 gtggtgcaga gcaaataacc gcgcgtcgcc agccaggccg ccgtctctac cctcagcgga
     1261 tgaatcgccc agaaaagcgc cgccagcgct gcggccgcat atcgtcccgt ccgttctttc
     1321 agaaacggca gacacagcag ataaaccagc atggaattaa caacatgcag cagcaggctg
     1381 gtcacatgcc atccgtgcgg attcatgccc cacgccttat agtccaaagc gcagctcagc
     1441 cagcttaacg gctgccagtg gccgaagaag aatgtactgc acatccactg ccaatgaacc
     1501 ggactcaggc cgcggtaata cggattacgc acaaaaagtc cgtcgtcatc ccagtacaga
     1561 aattcaccgc gcagaaccgg agaaaagcag accagagtca ccccggccac taacaccgcc
     1621 catagaagaa gtttcgagtt cagtttcata ccaaccgttt taaccgacac tcacaactcc
     1681 cgccagttca ttattttatc tgtccgcgcg gcataaacta tgcttttatc tctcccgaga
     1741 atatcaaggg agtccgttat gaaaactaaa accggaaaat caaaagcctc taaaggattt
     1801 acgctggttg aaattctgat tgtcgtcgcc attatcgggc tgattgccgc catcggaatt
     1861 ccatccttca ttaattcgcg tcaaaatgcc gaaaccaaca tgaaggcaat caatgttgct
     1921 gctgtcaacg ctgcaaaaga tcagtgggcg atcattaaca ataaagcaac cggtacgact
     1981 gtggcatgga ctaacattgc tccttatatc ggaaacagcg tcagcaatca ggcgggattg
     2041 acggtcggag gctctgccat aacgataaat cctgtcggca ccagcgcgtc ttactgatta
     2101 ccgccgggtc tccataacac tccggacacc ggccatcagt tctccgctct gaaagggttt
     2161 ctgaatgaaa tcagtgtgcg catgctgacc gaaccgctcc atcacctgtt cgcgcggata
     2221 tccgctcata aaaagcaccg gcattttagg gttgatatgc cggatgcgca gagccgcttc
     2281 aatcccgttc agccggggca tattcaaatc cataagcaat aaagtgatgt cccctgcatt
     2341 ttcggtatac agatcaacag tctcaaaccc gtcagaggct gtgattaccc ggaagccgaa
     2401 ccgttccagc agcgcggcag tgatgttgcg aatggcctct tcatcatcag ccactaacgc
     2461 cgtaccgtag ccgcgccata cttcgccgcc ttctgcctgc aactcttcag ctctcaggtc
     2521 gccatcctga gcttcgaccg ggaaatagat gcggaacacc gtgccgtgtc cgacctggct
     2581 tctgacctca atagctccgt tatgccgctg aaccacattg agtaccgagg ccaatccgag
     2641 tccgcgtccg gtgattttgg tggtgaacat cggatcaaag attctctgca gagtctcttc
     2701 attcataccg tcgccggtat cagaaacttc gagccggata tagcgtccgg acggcagacc
     2761 gccttgagga aaagtgcgct caagttcacc ggctttgagt tcaacctcat ggatgctgat
     2821 cgcgatcact ccggaacggt ctccgatcgc ttccgaggca ttggtgatga gattcatggc
     2881 cacctgacgg atctgcgaga tgtcaccgcg aatcaacgga acgttttcat ccaggcaata
     2941 ctgaatggat actttctttg aaatagagac atccagcaga gatcccattt ctttgacaac
     3001 cgagctgaga ttgatactgc tgacgaccag cgaggctttc ccggtataga ccagcatctg
     3061 atttgccagc tcagccgcac gcagagaggt ttttttaatt tcatcgagta cgccgccgtc
     3121 agagccgccg cattcgagca gcgccagctc ggcattacca agcacaaccg tcatcagatt
     3181 gttgaaatca tgtgcgatac cgctggccag cagccccaga ccggccatct tctggccgtc
     3241 ctggaatttc ttttccagaa tccgcatact gtcttccatg cgggcaatct gggttacatc
     3301 gcgcacggaa ccgctcagat aaactgcccg gccggattca tcatacacca catgcaaatg
     3361 gtcgctgaaa aaacgatact gcccgtcgcg atgaagaaaa cggtattcca ccagaccaag
     3421 ccactcacgt tctttgttcc gctgccgcag ctgtgcaatc agccggtcaa ttttctcccg
     3481 gtcttcggga tgaagatgct gaagcagctc ctgcggctcc agcgctttaa gctcttccgc
     3541 cgtgtatccc gtcagactga gacaggccgg gctcaaataa tcaaaccggc cgccgcgcag
     3601 atcaaaaccg aaaagaaact cgcgggaatg ctgcagaata ttccggacgc gcaaccggaa
     3661 catctcacag tcccgcgccg tctgccggat acgcgcattc cgccggagat gcatgaccgc
     3721 ccagaccagc gcggcaccga aaacaaacgt ccacagtgcg atgtaatgca gcgcgacgtc
     3781 gttatacatt aacgggaaag aggacagaat cgtccgttcc acgccgccga aaaacgaatt
     3841 attaagcagt ccggtcagat tcgcctccct gctttcgatc cagcataggc tgaacatgtt
     3901 tctcgtagca cgacgggcag acggaatggc tgaattcgac attcgaccgc cgtgtgatat
     3961 agctgtccac ctgctcccag tagtcctcgt cattgcggat cttattgcag tacgaacaga
     4021 tcggcagaag cccgcgcaac tgcttgacat cctcaagcgc agcttcgagc tgatgaactt
     4081 tattggccag catccgttca agaccgatca cacgcacgcc tacgtcgatt cgcgaacgca
     4141 gctcgcgccc gagaaacggc ttggtgacat agtcgtcggc accggcgctg agcccttcga
     4201 tcagattatc cacgccctgc tgtgccgaaa gaatgatgag ataaaaatac tcttcaggaa
     4261 atttttcacg caccttgcgg caaagttcgg ggccttccat ccgcggcatc agccagtcga
     4321 tgatacacag ccgcggcgga tcatcgctgc aaaggcgctc ccacgccgcc tgaccgtcgg
     4381 caaccgcttc gacctcaaaa ccccagccgc tcagcgtttt ttcgagccgc atgcgggtca
     4441 ctttctcatc ttccgcaatt agaatacgca tacttccccg tttatcccgc actctaaacc
     4501 agcataagcc cgccaacaag ccgcttttgt ccggccttac acaaccagaa aatcaaccgc
     4561 gtgaacccag tcctgcttga gccggtaaag ctgctgggta agctccgtcg cttcagccgc
     4621 gcggcctgcc gcctgcaatt cacggcggcg gcgggtcaaa gcctgccgcc agagcgccat
     4681 cacaatatcc tgagccgcct tggccggcgg aacatccccg cgaagcttca tctcctcaac
     4741 ctgaatccgt gccgccagcc gctgggcctc cgggcggtcg tcggggatac tgtccatcag
     4801 cgccgccggg tcttcgagca tcagctccag cagcaggcgg cagtccggat cggtaagatg
     4861 cgcgggcgga agatggtcgg ccaccaccgg catcacttca gccggatgca gaaacagcat
     4921 ctggatcagc gccatttctt catcaggatg ttttaccggt gcgaccggaa cttccggagc
     4981 tgcaaccgga gccgggtgct cttttttacg cgctaaatcg agccggaatg cagccggcga
     5041 gagacccagc cgcccggcgg catgcgtcac catgcggtca cgctgaaccg cgcccggtgc
     5101 ctgagcaatc agagcctgca cggcgcgggc cgtgcgcatc actccggctt ccgttttcag
     5161 attttcatgc gccgccatta catcgatcag aaaatccaac gcggaaacag cgccggcaat
     5221 ccgcgaacgc agagcttccg cgccatgatc gcgcaccagt gaatccggat cttcgccttc
     5281 cggaagcgac gcgacgcgca cgctcagctc ctcggcgata aatgccgcag aagatttcag
     5341 cgcggccttc tgcccggcgg cgtcggcatc cagcaccaga attacttcgt cggcatagcg
     5401 ccggatcata cgggcgtggt ccgccgtcag cgcggtgccc tgcgaagcaa ccacattggc
     5461 aatgcccgct tcgtggcagc ggatggcgtc gagctggcct tcgcagacaa tggccgtgcg
     5521 gctgtcggcc atcgcgcggc gggctttatc aatggcgaac agcacacggc tcttgtgaaa
     5581 aagcggcgtt tccggactgt tgacatactt gccgcttttt tcgtctttga cgatggcgcg
     5641 cccgctgaag ccaattaccc ggcctgcttc atcgcggatc ggaaacatca ggcggttgcg
     5701 gaagcggtca taatatccgc tgcgcttatc ggacttgatc atcagcccgg cctgctccat
     5761 gagttcgagc ggaatctttt tctgcagcgc ccatttttcg agcgcatcga aacggtcggg
     5821 cgcaaagccg atctggaatt cttttacggt ttccggcttc agcgaacgag tggagaggta
     5881 cgcccggccc gcttcgcccg attcatgctc cagcagaatc cggtgataga gctgagcaac
     5941 gccttcgtga atctggaaaa gcttccgttt gtccgcgccg ctcccttcgc cgccctcttc
     6001 gaggctcagt tccatgccgg cgcggtcagc cagcatttgc aaagcggtca tgaagtcgac
     6061 tttttcatat tcctgaacaa agcggatcac atcgccgccc gcaccgcagc cgaagcaatg
     6121 ataaatctgg cggtcaggac tgatggtaaa cgagggactt ttttccttat ggaacggaca
     6181 gagtgcttta aagcgcgtcc cggcgttctt gatcgagaga tacgagccga tcacatcagc
     6241 aatatcattg cgcgtacgga cttcttcgat ggtttctctg ggaatctgct tcatgaatta
     6301 ccgggtaatg tcgtcaatac ggttctgcat gtcctttttc aggacaggca gctctccgat
     6361 gcgcggcgag gcccacggcg taagcgtatt gcaaacccat ccgccgatgg cggatttctc
     6421 agaaagcacc cggtacaggg cgtcgttcgg catcaaactg agaccgacaa aaaccgtcag
     6481 tcccagaaga atgccgcgca gcattccgac cagcacgccg ccgactttat cagcgttcgt
     6541 gtccgccaga tccttcagca tctgccggaa cagaatacgc agcaggacaa acagaagcac
     6601 cgacgccagt ataagaaccg ccggcacgat aaaccgcagc gcagccgccg gcagattcca
     6661 atggtctttc gcaagatttt ccagctgcgg atagaaaaag cagaagccgg tcagcagtgc
     6721 gagcagagtc accacgttag ccagctcgcc ggaaagtccg ttccataccc ctcgaaccgc
     6781 aaaaaaaagg acaaaaagga caaacaggat atcaatcgta taaagcattt cgggaaacca
     6841 catatttttc acctcgtgcc ggttttatag cacatccgtt tcaggaacgt agagattttt
     6901 ccgattgccg caaaaaacag aaattcggta gcttttttcc ccggcccggg agaaaaattt
     6961 ataaaaaata aacagccgga gaggcttttt tcagaagggt ttagcgggca tggatttaca
     7021 cataagacac aatatgttgt ggtttcgttt gacagccata cagcatttgg ataatttttg
     7081 cccgtcgttc aacgaacgtt aaaataaaaa gccaacttat ctacaggttg tcaacagggg
     7141 gaaatttcta tggctgaaac aattacatca aaagacgcac ttttcgaagg attccgcaaa
     7201 cgctcagggc tgatcgtgcc tttcagccat aaaaaaattg aaatggcgat tcacctcgca
     7261 gttgaggaag ttgcccgcaa gcagaacact ccgcgcaacg agggtcttgc tccgaaaatc
     7321 acagctcagg ttctcgaaca gctcaacaac ccccagtctg agtttttcgt gcgccccgaa
     7381 gcggacggca agcgcatccc gcatatcgaa gacgtacagg atctggttga aatcctgctg
     7441 gcggaaagcg gcgaaacgct ggttgttgcc gcctacaagc gctaccgcaa acagcgcgaa
     7501 atcgcccgca acagcatccg cgtgcgcgac ggcagcatta aaaaagacaa ggtagacgtc
     7561 actgacgcca gcctgctgct ggtcgaatcc cattcctccg aagttaccct gccgtgggac
     7621 cgcaaacgca ttgttaaaca gatcctcgac aagacagacc tcgctgtcga caccgccatc
     7681 agcatcgcga aagcggtcga aaaccgcatc atcgcgagca acatgaaggc cgtcaacacg
     7741 acgctcatcc gcgaaatggt caataacgag ctggcggaac gcggattttc agctcagttg
     7801 cgcgacctct ccctctacgg cgtaccgctc gactacgtcg aaaaactgat gttcaccaag
     7861 tccaccgaaa acagcaacat cgtcaacaac aaccctgaag ccgtaaatct cggcatcgcc
     7921 gaactggtgc tcaaacagtg ggcgcttgac accattttca ctcccgacat caaacgcgcg
     7981 cacaataccg gcgccgtgca tctgcacgac ctcggctatc cgcaccgcgt ttactgctcc
     8041 agccactcca ttgaatacgt caaaaaatac ggcctcaaag gactgatcaa ccttaacacc
     8101 gaatccagcc cggcgaaaac cgcctccgtg ctgaccggcc acctcaacac cttcctcgct
     8161 tccatgcagg ccaactacgc cggcgcgctc ggcattgcct acatcaacat tctctacgcg
     8221 ccgtacctcg aaggcatgaa cgcccgcgaa ctcaaacaga ttgcgcagga actgatcttc
     8281 aacggctcgc agaacgcgtt cagccgcggc ggacagaccc tgttcctgga tttcaacatt
     8341 cattccggcg ttccgggcta catgaaaacc gttccggcaa tcggcccggg cggcaaatat
     8401 atgctccgtc tgaaatccgg cctgaaagtc cgtctcgaag aagttctgcg cgacgaccgc
     8461 gacatcggcg gctacaggct gatggatctt tatcttcaag aagccgacgg ctcacagcgc
     8521 ctcgtcctgc gcgaaattgc cggcaaagaa ggtctggaat acgacgccgc tgttgccgcc
     8581 gacctcgaag cccgcggcga aaaaatcgtc acctacggcg attacaccaa ggaagcgcag
     8641 gatttctgcc gctcgctgct ggaagtctgg ggcgacggcg acagcaacgg ccgcatcttc
     8701 gaattcccga aatgcgactt ccacatcagc gacgaaacct tcgtcgatcc cgagcagtat
     8761 cagatttatc aggccgcctg ccagctggcc gcgaagaacg gctcgaccta cttcatcttc
     8821 gaccgcgacg aagtcacgct gtcggcctgc tgccgcctgc gtaccaccat cagcgacaac
     8881 cgcatgctgc gccaccctga aaatatgcgc ttctgcggat tccagaacgt caccatcaac
     8941 attccgcagg cggcttaccg cgcggcgcgt aaaggcggcg acatcctgaa gaacttcttc
     9001 gatgaaatcg acgccaccat ggaactctgc gcgcaggctc acctgcagaa aaaagccaag
     9061 atcgcccaga tgatgagcgg taacggccat ccgctctggc agatcggcaa agaatcgaac
     9121 gacggcaaac cgtacgtcaa cctcgatacc tgcacctaca ttctcggtct gatcggcgtc
     9181 aacgacaccg tcaaattcct gatcggccag gaactgcacg acagcgacga agcccagcag
     9241 ttcggactgc ggatcgtagc ccacatgtac acccgcacca aaaagctgag caagaagtac
     9301 aacctcaagt tcacactcga agagtcgccg gccgaaagcg ccgcgcgccg tctggccaag
     9361 accgacctca tctatttcca cgatgaagcc aaagatgtag tcaaaggtga aaccgaagat
     9421 gtctgctact acaccaactc cattcatctc accgccgacg ctccggtcgg tctggttcag
     9481 cgcatcgtgg agcagtccaa atatcacagc ctgatcgaat ccggcgccat cacccacgcc
     9541 ttcgtcggcg aagaacagcc ttcggccgct tccatcgaga agctcatgac cgaaaccttc
     9601 ttccgcacgc agtcggcgca ggtctgcatc tcgccggaat tcaccttctg caacaactgc
     9661 caccatcagg tgcgcggact gatcgaaacc tgccccgcct gccagtccgc cgacgttgtc
     9721 ggcgaaaccc gcgtggtcgg ctacttcagc aaagtgcaga actggaacaa atccaaacgc
     9781 tacggcgaac tcaaagcccg ccagcgcggc caatacgcga ttgaaaccgc cgaaccctgc
     9841 acggaaaaga aaccggtgtc cgtcaaaggc tgaaccgttt gaaccaagga gatttaccca
     9901 tgaaaaagcc ctttaaaatt aaagtgttcg gcaaagaagg ctgcgccaaa tgcaaaatgc
     9961 tcaaccagcg cctcgacaaa ctgcttgaag aaaaagagtg gtccgacttc gaaaaagaat
    10021 actgcgatgt cgaaacggtt gacggcctcg tcgccttcgc ttccgccgaa tgcgtaaacc
    10081 cccagcggat tccggccctg ctcgtcacgc gccggcagga agaaaccgaa cgctacgttc
    10141 cggttccgac ccgcaacccg cagccgcgcg accccgtctg cggcaaatca aagctctacc
    10201 agtacgtcgg cctgcaaacc gattacagcg acgaaggtaa aggaatcatc tcccccagga
    10261 tgatcactgc ggttctcgaa gaggcccgcg ggtgatgccc cccggagcgt cagctccgcg
    10321 taagcaggcg gctctgccgc aaggtgagtc gcctgtttac gcatttttag aaaagccctc
    10381 gatgatcgac taccccggct tcctgtgcgg ggtttttttc atcagcggct gtaacttctc
    10441 ctgcggcttc tgccataacg ccccgctcat gggcaaaaag cagaaaggcc tgccgtggag
    10501 ccgtctcgac gaactctgcc gcaaattcaa aaccgaatgg gtggatgccg tctgcatcac
    10561 cggcggcgaa ccgacactgg ctcccgacct gatcgcgctc attgaatttt tccgcggcta
    10621 cggatttaaa atcaaactcg actccaacgg cgcgcggcct gaagttctga aagaatgcct
    10681 gccgctggta aactacgttg ccatggacat taaagccgga ctctccggct accccgaact
    10741 cgtcggcttc acacagaccg aaaaaatcgc cgaatccgtc aaactcatca tgagcagcgg
    10801 tctggactac gaattccgca ccaccgtcat cgaaccattc cacactgacg agcagatgcg
    10861 cgaaatcggc gaactgatcc agggcgcccg ccgctactgc atccagccct tcatcccgaa
    10921 agacaacctg cccgacccgc gcctgcgcga aaccaaacgc accactcccg cccgcctccg
    10981 tcagatcgaa aaacttatga aaccctacgc cagcgaaatc atcgtccgcg gagcgtagcc
    11041 cctcaacctc tccgcaatca gcaaccatct tttggaaatt ccatcaaaac ttaaggtgac
    11101 acggcttgta tgcgttctta gaaaaagata aaaaactcga aagggtttga tgcatcacaa
    11161 aaaaaccatt cctcatgagg aataacgcgg aaggacatcc gatgaatacg atgagaaaaa
    11221 tgttttactc actttgtgta gcggggctag ccctatcagc atcaattaag gccgaacctg
    11281 ttagcctgcc tattccgggc gaaggatgga atctttcttt cgatgctccg cccatcatct
    11341 ggaccaagtg tgataagacg agctctggtg tcagctacct cggctcatcc gaaaacggag
    11401 ttgttatttc attttttgtt gaggaaccgg acggcgaatc ccgcggtaat aaggcatgtg
    11461 cggattacta ctggcctctt ggcaaaaaga acccgatgat tgatcagtca accgtagaaa
    11521 tcgaagaaac taaagatttc gtcaaagtgt cgtacagatt acgggggaag tacaaaggca
    11581 ccgcgatcga tatgccgaac atcaaccttt actttgaata ccaaggaaaa tgggttgatg
    11641 ttcacatctc gaaacttcca ttcgaggacg aggattcggc ccgaaaaata ctggatgcct
    11701 ttgtagagtc tctaaaatgc gaaatgatct ctgggagaag tgctccggcc aacccggaaa
    11761 aacttgtctt ccagacacgt tctttccctc tgccacaaca cggaaccttc acccttgata
    11821 ttccaacttc atggaaacag cgtttccaac aaccaaagga tgaaaccctc cccccaacaa
    11881 tcaccttttc tccaaaacag ggtgatgaat tcgaagtaat gattactcca atctggagcc
    11941 caaaaaatga cctttcattc accaagccag aatccgtaag gcaacttatc gactctgatc
    12001 ttcaacgcat gctgcctgat gccgtggaaa agcaggttcc tattcaggag tttaaaggaa
    12061 tcgacggctc cggatattat ttctttgtca cagataaagc cacaaagccg ggcgaatatc
    12121 cgtacgcagt tcgcgcagga atcagcaccg gagatttgct gctgtacgtc acggttctgt
    12181 gccgatcaaa agacggcggt gaaatcgaag ctgttatcaa agcgcttgag acggcaaaac
    12241 aggaaaaaaa gtaaaggtag ccgttatcgt ccgctcaacg cttgggtgca ttcaaaagaa
    12301 aagaagccat gcaaagcagt cttgttttgc tataaaaatt gcatttagtc cgtcggctgt
    12361 tgtcatgcgg cgcagcagga gaaaaatatg ctgagcaaag aaaatgctgt tttaattttt
    12421 atcgatgtac agggccggct tgcggagctg gtggacggcg cggatgcgct gtttaaaaat
    12481 ctccgccgcc tgctggaggg catgaaggcg ctggacgttc cggtaattgt caccgaacag
    12541 attccggaaa agctcggtcc gacccgcgat gaattccagc cgttcatcac agcgccgccg
    12601 gttaccaaaa cctcgttcag ctgctgcggc gaaccggcgt ttttgaaatc gctgaaaaaa
    12661 acaggccgcc gccacgtcat tctctgcggc attgaaaccc atgtctgcgt ttaccagacg
    12721 gcgatgcatc tgctgaaatc gggcttcaaa gtttatgccg tcgccgatgc ggttt
//
LOCUS       DAOPZO010000046        12540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_5614_length_12540_cov_11.164517, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000046 DAOPZO010000000
VERSION     DAOPZO010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 12540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12540
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5614_length_12540_cov_11.164517"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..929
                     /locus_tag="PKI68_04440"
     CDS             150..929
                     /locus_tag="PKI68_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52972.1"
                     /translation="MVKDLSSFRNTLSGFLSWQPQTVTTFQPSLRSWRVCFRPLAMLQ
                     ANFLFQKALLLFGVVASLQPLCRCREQPQTTTSSGGGRKLRELLGERFEVEIHQLGLA
                     VFRFFRERDKAAPRIKINRIFVGVGGNKTAACFVPSDKQNFDVIQQLCAKPLFLERSS
                     DSKASEFYGRITFITPFPVRQKPLQFAELGFRRELLNSDPVVQYTEIRNNRISVSDQI
                     GNSQKVILMRKGVFEQELIQVGIAAVEICGNTRQAFDFTVF"
     gene            complement(1186..1632)
                     /locus_tag="PKI68_04445"
     CDS             complement(1186..1632)
                     /locus_tag="PKI68_04445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HNX52973.1"
                     /translation="MDHLTPAKRSWNMSRIRGKDTKPEILVRSMLHRAGYRFRKNVSS
                     LPGRPDIVLPKYRTVIFVHGCFWHRHKGCKDATTPKTNKAFWKKKFERNVANDKKHAR
                     DLKKLGWRVITVWSCDIAKNPNWVVRKIENHICQNPKQHLARSVAI"
     gene            1650..2444
                     /locus_tag="PKI68_04450"
     CDS             1650..2444
                     /locus_tag="PKI68_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX52974.1"
                     /translation="MPAKGQSLMPFADDIQYLIKAGWTRSEAFGTKAMTAGNGNPAVR
                     IAELPLPLVAAFQSLSAVFYFPVEAGNTVRGIRSVSVRPATSRSSGIALTCGLLGKNI
                     QSVRRPRKVADDLQAGSVCRGNAAETYRVSMAHILIIDDDDSIRDVLKRFLERFGYIV
                     SLASSGREGLRLLQQKKTDIVITDIFMPEMDGLEVILEIRKRCHRLSGSLPVIAISGG
                     ILTMSAKPISFLDQARAFGAARVFQKPLDFAGIQTAIQELLPAEQA"
     gene            2658..2888
                     /locus_tag="PKI68_04455"
     CDS             2658..2888
                     /locus_tag="PKI68_04455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52975.1"
                     /translation="MAYKTKVRKIGNSLGIVLPKEALQAMKVEEGATLYITEAPKGAV
                     QVTPDNEHFGEMMKIAERGMRKYRNALRELAK"
     gene            2885..3280
                     /locus_tag="PKI68_04460"
     CDS             2885..3280
                     /locus_tag="PKI68_04460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HNX52976.1"
                     /translation="MKEPVWVPAEVILAIQEELLARFGGLSGLRDEGLLDSALNRPRQ
                     VFHYGSPALFDLAAEYALGIVKNHPFLDGNKRAGFMAAYTFLGVNGYDLNAPEAEAVM
                     QTVALAAGEIDEKDYAAWLKASCQKRKRP"
     gene            complement(3349..4560)
                     /gene="trpB"
                     /locus_tag="PKI68_04465"
     CDS             complement(3349..4560)
                     /gene="trpB"
                     /locus_tag="PKI68_04465"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNX52977.1"
                     /translation="MKKDYRDYLQNTPDANGNFGPYGGAYIPPQLVGPMKEIHEAYLK
                     ISRSHRFIEELRSIRKHYQGRPTPVYHAARLSGKCGGAQIYLKREDLNHTGAHKINHC
                     MGEGLLAKYMGKKKLIAETGAGQHGVALATAAAYFGLECEIHMGEVDIAKEHPNVVRM
                     KLLGAKVVPVSHGLKTLKEAVDSAFQAYLKDPVSSIYCIGSVVGPHPFPMMVRDFQRV
                     VGIEAREQFFEMTGGMPDVVTACVGGGSNAMGIFSAFLNDPCEIWGVEPLGRGPKTGD
                     HAATATYGMPGIIHGFKCYLLQDEKGEPSPVYSIASGLDYPGVGPEHSHLKDEGRVQY
                     TAIDDKECLNGFFTLCRSEGIIPALESAHAVAFAMKKAKEMKSGTILVNLSGRGDKDL
                     DFVVDNYGYGD"
     gene            4755..5402
                     /locus_tag="PKI68_04470"
     CDS             4755..5402
                     /locus_tag="PKI68_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNX52978.1"
                     /translation="MNILHVCANPKPIEQSVSKQLAARFFGKLMELNPDVELNNVDLY
                     QDKPPYYSNDLYRRCWMPVFDPAYEPSKAEESALNYAQKQADAFKAADVLVLTMPMWN
                     FTVPGVMKAWMDQVLSPGLTFTVDAEGPKPLHNVTSIILLVASGGVYKENDDRDALTR
                     QVRSAFGFIGISDVRIAWADGQNPLFFNDSEVRRETAFEAAEELAEELAEESAAG"
     gene            5413..6045
                     /locus_tag="PKI68_04475"
     CDS             5413..6045
                     /locus_tag="PKI68_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNX52979.1"
                     /translation="MSVFVKICGICSRSDLEQICALEPDAIGFVFWPRAKRYVRPEQV
                     ASWLRSIPEQIKKVGVFVEPPPAEVEAVAIACHLDVIQVHLISNDWRIDRPLFRGLET
                     WLSPRMGEGVGRNIIHAVDPEPSVLLADSFDPNTIGGTGKLSSWERALAMKTETGKPV
                     MLAGGLTPENVRDAITAVQPWGVDVSSGVEKEPGVKDIRKVKQFIERVRR"
     gene            6042..7241
                     /gene="trpB"
                     /locus_tag="PKI68_04480"
     CDS             6042..7241
                     /gene="trpB"
                     /locus_tag="PKI68_04480"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNX52980.1"
                     /translation="MKPVQPDSQGHFGPYGGIFVPETLIEPLRELTRVYKSAQKDPVF
                     KKELEYYLREYVGRPSPLWFARRMTAQLGGPKIYLKREDLNHTGAHKINNVIGQALLA
                     KRLGKTRLIAETGAGQHGVATATAAALFGMECTVYMGKVDMERQALNVFRMESLGAKV
                     VAVTAGQQTLKEAINEAMRDWVSNLKNTHYVLGTAFGPHPYPMIVRDFQSVIGKEARK
                     QILKAEGRLPTAIVACVGGGSNAIGIFHSFIKDEGVRLIGVEAGGLGIEPGKHAARFA
                     GGKVGVLQGTKSYVLQDGDGQILLTHSVSAGLDYASVGPEHAMLHDTGRAEYTYIEDA
                     DAVAGFDRLAQWEGILPALESSHAIAWVIKNAHTFTKDDLIIINVSGRGDKDVQQIAE
                     WKKENQQ"
     gene            7254..8117
                     /locus_tag="PKI68_04485"
     CDS             7254..8117
                     /locus_tag="PKI68_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52981.1"
                     /translation="MCKRRAMNCWMGLISGIFILNAMAETLSPPEKKEYSTGEILEEQ
                     FALAGKIIKVRFNRIYYVSKQPNGMYTGNLRSMVTLSGNYYSDPAGIQIRFPQEGFGY
                     ISKFLPKPGVSYEEEENRVYESDSGTLYVQVSNEARPGFVAIGDQYRREDDQEEEEYR
                     WSVKTEPPDLSDRHTVSVSDVWLFPEYLNGRTVELQFRSIGQITQTAVNEYRVSVSDG
                     RGCLSVPLIVPPAGRAFFKEIELSQRELEGSYQVSRVYVSVTINANGTTVLTAQGRRM
                     SGSGDSAEYRW"
     assembly_gap    8118..8217
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8218..9060
                     /locus_tag="PKI68_04490"
     CDS             <8218..9060
                     /locus_tag="PKI68_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNX52982.1"
                     /translation="SACSEDARVTVELSSVNRKQLDLVLRLPPQIAALESRVQKIIQE
                     HISRGRISGNVQVESAAGGALTRIDEKRAAETVGRLRRAAKKLNLADDLGASVLLQIP
                     GLIKMPSAEQNPEGILQTLDKALIAALKKLNAMRMREGKTLEIDLLARLKLLEQMLAV
                     IKSRAPQITSGYRKKLFSGLESAGLKNLAADERIIKEIALFAERSDISEEITRLASHI
                     QQFKKILRSGEPAGRPLDFLAQEFFREINTIGSKANDLKTTGQVVAFKTELERIREQI
                     QNVE"
     gene            9050..9655
                     /gene="gmk"
                     /locus_tag="PKI68_04495"
     CDS             9050..9655
                     /gene="gmk"
                     /locus_tag="PKI68_04495"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNX52983.1"
                     /translation="MSNKVTPLLLVISAPSGAGKSSLCSRLVEKFPGMVYSISCTTRA
                     PRGSEQNGVHYHFLSEEEFCARIAAGEFLEHARVHGNRYGTLKQTVYDALSQGRDIIM
                     DIDVQGAQQIRAACAKMRDDDAIKRSFVDIFIAPPSMEELRRRLCGRSTDSAEVIERR
                     MRNATEEMKHQPLYQHTVINDDFEKAADELSAIIESEHHNR"
     gene            9809..9940
                     /locus_tag="PKI68_04500"
     CDS             9809..9940
                     /locus_tag="PKI68_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52984.1"
                     /translation="MAAKKVAKKKVAKKAVKKVAKKVAKKAVKKVAKKVAKKVAKKK"
     gene            10071..10526
                     /gene="nrdR"
                     /locus_tag="PKI68_04505"
     CDS             10071..10526
                     /gene="nrdR"
                     /locus_tag="PKI68_04505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNX52985.1"
                     /translation="MKCPKCEHRENKVIDSREVREGTAIRRRRLCLNCGHRFTTYEEV
                     LRAALQVIKRDGRREELSREKLMQSIVVACQKRPISIQQIEQMTNNVITQSEAEYERE
                     IPSTALGTKVMALLEKLDEVAYVRFASVYRRFKDVNQFMNEAERLIGRE"
     gene            10523..11176
                     /locus_tag="PKI68_04510"
     CDS             10523..11176
                     /locus_tag="PKI68_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52986.1"
                     /translation="MIDTHQSLRLDERRLWSGYVQQLFQTLDISDRASAGDITESVAV
                     FCQQHANVSSHALSLLMARSFCITGDAEAAGRILRHDREHSPHAGSWLEVLSAGYPFP
                     ELYPLFSSRALRPQSLTSAGALWVLDFDKVQLTDADRHELILFQTVRVLTEKVSNVWK
                     KSAGRGTLGVKGLPRLMRFVRGRRMPPQLPRHIEDVLTRCARKHGWSHTPAILLLDL"
     gene            11194..>12540
                     /gene="lnt"
                     /locus_tag="PKI68_04515"
     CDS             11194..>12540
                     /gene="lnt"
                     /locus_tag="PKI68_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNX52987.1"
                     /translation="MKKQLSIIASICTGLLLFLSFPPFEISFCGWFALIPLIFACAGT
                     APRRAAFLGWLAGAVFFLSSLFWLRHVTWLGTAGLALYCALYFMPFAVFVSLRRDGWR
                     GTNGFKNIGWMLGAAAVWAAGEYLRATVITGFPWNPLGVSQYKQLACIQIAEWGGVYA
                     VSALMVFVNAAFAVTILQYATGLRRRSRMHLEIMFALLTVALAWSVGIQKLLHRPAGG
                     TPVRAALIQTDVPEVGNWEKADPEVVYEKLERLTALATRSADPDLIIWPETALPDFVR
                     FSERSAGLVRKLVTNGVPLLAGSMDAVWRADGNPDYYNASMLFNTRGELLGSYYKQHL
                     VLFGEYIPFEGKIPLINALTPIGSSFSSGRAATLFRRPGDDRGFCVLICFEDSLPYLA
                     RKAARAGATFLVNQTNDSWFDPDCGSVQHLANAVFRCIETRLPMLRCANTGITCSID"
BASE COUNT         2743 a         3323 c         3663 g         2711 t
ORIGIN      
        1 ggctgaccgt tgtggaacgt agagatggaa agatcgtagt tttttctgat gaatgcgcct
       61 gcgaatacac ttcgttttac gcgtttcagg agggcgaaca gcctctttgc cattagacag
      121 aactcatcag tgcagatcag tgggaatcgg tggttaaaga cctctccagt tttcggaata
      181 cgttgtccgg ctttttgagc tggcagcccc aaacggtgac gacgttccag ccgagcttgc
      241 gaagctggcg ggtgtgtttc aggccattag cgatgttgca ggcgaatttt ttgttccaga
      301 aggctttgtt gcttttcggc gtcgtggcgt ctttacagcc tttatgccgg tgccgggaac
      361 agccgcagac cacgacgtca tcgggcggcg gaagaaagtt gcgcgagctg ctcggcgaac
      421 ggtttgaggt cgaaatacat cagctcggtt tggctgtctt ccgatttttc cgagagcggg
      481 acaaagccgc tccgcgcata aaaatcaacc gcatttttgt aggcgtcggc ggtaataaaa
      541 cggcagcctg ttttgttccc agtgataaac agaattttga tgtaatccag cagttgtgcg
      601 ccaagcccct gtttcttgaa cgcagctccg acagcaaggc gtccgagttt tacggccgga
      661 taacttttat aacaccgttt ccggtgaggc agaaaccgtt gcagtttgcg gaactgggat
      721 ttcgtcggga actgctcaac agcgatccgg tcgttcaata cactgaaata cgcaacaacc
      781 gcatcagcgt gtctgatcag ataggtaaca gccagaaggt cattctgatg aggaagggcg
      841 tcttcgagca ggaactcatt caggtcggca tcgccgcagt cgaaatttgc gggaacacca
      901 gacaggcgtt cgattttaca gtcttctagc cgcaacatgg gagtccgctg tgtttattga
      961 cagcgcgata aacggactga gcccggcgaa cctcatccaa ggacacccgg gtcggggttt
     1021 tgatctgttc gttaaaccgg cgggcgtcat ttccgtgcag gaacggagtt tctttaatag
     1081 gtctggccat aattttctcc cttacacggg gcgcgcattg aaccatattt ttccgaagct
     1141 tcaaccctta aatcgcaacg atattagctg cagaagacgg ttctgtcaaa ttgcgacaga
     1201 ccgcgcaagg tgctgttttg gattttggca aatgtggttt tcgattttcc ggacgaccca
     1261 atttggattt tttgcgatat cacagctcca aacggtaatc acccgccagc cgagcttttt
     1321 tagatcacgg gcgtgttttt tatcgttggc gacgttgcgc tcgaatttct ttttccagaa
     1381 ggctttgttg gttttcggag tcgtggcgtc tttacagcct ttatgccgat gccagaaaca
     1441 gccgtggacg aatataaccg tccggtattt tggcagaacg atgtccggcc tgccgggcag
     1501 ggagctgaca tttttgcgga agcggtatcc ggcgcggtgg agcatggagc ggacgaggat
     1561 ttccggcttc gtgtctttgc cgcgtatccg cgacatattc cacgagcgtt tcgctggagt
     1621 caggtgatcc atgcgcgaag cataccgcct tgccggccaa aggccaaagt ctaatgccat
     1681 ttgcggatga tattcagtat ttgatcaagg caggctggac gcgaagcgag gctttcggaa
     1741 cgaaagcaat gaccgcaggg aatggcaacc ctgcggtacg tatcgcagag ctgccgctcc
     1801 ctttggtcgc tgctttccaa tctctgtctg ctgttttcta ttttcccgta gaagccggga
     1861 acactgtgcg gggaatacgc tctgtttctg tcaggccggc tacttcacga agttcaggca
     1921 ttgccctgac atgcggactt ctcgggaaaa acatacagtc tgtgcgaagg cctcgaaaag
     1981 ttgcggatga cttgcaggcg gggagcgtct gtcggggaaa tgcagctgaa acatacaggg
     2041 tttctatggc gcatattctg attatagatg acgacgattc gatccgggac gtactcaaac
     2101 ggttcctgga gcggttcggc tatatcgttt ctctggcctc cagcggtcgc gaagggctgc
     2161 gtctgctgca gcagaaaaag acagacatcg tgattacgga catttttatg cccgaaatgg
     2221 acgggctgga ggtcattctc gagatcagaa aacggtgtca caggctgtcc ggcagcctcc
     2281 cggtcatcgc gatatccggc ggcatcctga cgatgagcgc caaaccgatc agttttctgg
     2341 atcaggcaag agcgttcggt gccgcgcggg tttttcaaaa gccgcttgat tttgccggca
     2401 tacaaaccgc gattcaggag ctgctgccgg cggagcaggc ctgactgccg gcaaccctta
     2461 ttccgcttcc ggttttttca cgcgaattgc aaacggaagg acgcggacgg tgcagtaaag
     2521 ccggaacttt ggtccggctt attaaaggaa agatgccgga tccgcgacac atagcggcgg
     2581 agttcgggcc gtcctgcgga acggcgctcc ggcagtccgg gcttgctaag gccgcatgtt
     2641 ataactaccg ttcccacatg gcctacaaaa ctaaagttcg gaagatcggc aattcgctgg
     2701 gaatcgtgct tcccaaggaa gctctgcagg cgatgaaggt ggaagagggc gcgacgcttt
     2761 atattaccga agcgcccaaa ggggccgtgc aggtgacgcc cgacaacgaa cacttcggcg
     2821 agatgatgaa aattgctgaa cgcggcatgc ggaaataccg caacgctctg cgggaactgg
     2881 caaaatgaaa gagccggttt gggttcccgc agaggttatc ctcgcaattc aagaggaact
     2941 tttggcccgg ttcggaggac tgtccggcct gcgcgacgag gggctgctgg actccgcgct
     3001 gaatcgtccg cggcaagtat tccattatgg ttcaccggca ctgtttgatc tggcggcgga
     3061 atatgcgctg ggcatcgtga agaaccaccc gtttcttgac ggcaacaagc gcgccggatt
     3121 catggcggcc tacacttttc tcggcgtgaa cggatatgat ttgaatgcac ctgaagcgga
     3181 agcggtaatg caaacggtgg cgctggccgc cggagagatc gatgaaaagg attacgccgc
     3241 ctggctcaag gcttcctgtc agaagagaaa aagaccatag gctttaggct ggaggcgtta
     3301 ggttcagaaa actttgcgag cctatggcct aaagtctaat gccttttcct aatctccgta
     3361 gccgtagtta tccaccacga agtcgaggtc tttgtcgccg cggccggaga ggttgaccag
     3421 aatcgtgccg gatttcatct ctttggcttt tttcatggcg aaggcaacgg cgtgcgcgct
     3481 ttcgagcgcc ggaataatcc cttcgctgcg gcagagagtg aaaaatccgt tgaggcattc
     3541 cttgtcgtcg atggcggtgt actgaacgcg gccttcgtct ttaaggtggc tgtgttcggg
     3601 gccgacgccg gggtagtcga gtccgctggc gatggagtag accggcgacg gttcgccttt
     3661 ttcgtcctgc agcaggtagc acttgaagcc gtggatgatg ccgggcatgc cgtaggtggc
     3721 ggtggcggcg tggtcgccgg tcttcgggcc gcggcccagc ggttcaacgc cccagatttc
     3781 gcacgggtcg ttgagaaatg ccgagaaaat gcccatggcg ttgctgccgc cgccgacgca
     3841 ggcggtgacg acgtccggca ttccgccggt catttcgaag aactgttcgc gcgcttcgat
     3901 gccgacgacg cgctggaagt cgcgcaccat catgggaaac ggatgcgggc cgacgacgga
     3961 gccgatgcaa taaatggagc tgaccggatc tttgaggtac gcctgaaagg cggagtcgac
     4021 ggcttctttg agggttttga gtccgtggga aaccggaacc actttggcgc cgagcagttt
     4081 catgcgtacg acgttcggat gttctttggc gatatcgact tcgcccatgt ggatttcgca
     4141 ttcgaggccg aagtaggcgg cggcggtggc gagtgcaacg ccgtgctggc ccgcgccggt
     4201 ttcagcgatg agctttttct tgcccatgta tttggccagc aggccttcgc ccatgcagtg
     4261 gttgattttg tgcgcgccgg tgtggttgag gtcttcgcgt ttgaggtaaa tctgggcgcc
     4321 gccgcatttt ccggagaggc gcgcggcgtg gtaaacgggc gtcggacggc cctgatagtg
     4381 tttgcggatg gagcgcagtt cttcgatgaa gcggtgcgag cggctgatct taaggtaggc
     4441 ttcgtggatt tctttcatcg ggccgacgag ctgcggcggg atataggcgc cgccgtacgg
     4501 tccgaagttg ccgttggcgt ccggggtatt ctgcaggtaa tcgcggtaat cctttttcat
     4561 gccggctcct tatctagatc gttaagaaaa gagccgggag cgtaagacaa aaagcgtccg
     4621 ggggcaagac cggccccgcg gaaatcagaa tcatgcccgc ttcctaataa gaaacgcttg
     4681 tgccgcgccc cgcgccgctg ttacagtcgc gcggttgatt ttataacccg aacgaaacga
     4741 aggagtatca gcgaatgaat attttgcacg tgtgcgccaa cccgaagccc atcgagcagt
     4801 ctgtctccaa acagctcgcc gcgcgctttt tcggcaaact gatggaactc aatcccgacg
     4861 ttgaactcaa caacgttgac ctctatcagg acaaaccgcc gtactactcc aacgacctct
     4921 accgccgctg ctggatgccg gtctttgacc ccgcctatga accgagcaaa gccgaagagt
     4981 ccgccctgaa ctatgcccag aagcaggccg acgccttcaa agcggctgac gttctggtac
     5041 tgaccatgcc gatgtggaac ttcaccgtcc ccggcgtgat gaaagcctgg atggatcagg
     5101 ttctttcgcc cggcctgacc ttcacggtcg acgccgaagg cccgaaaccg ctgcataacg
     5161 tcaccagcat catcctgctg gtcgcctccg gcggcgtata taaggaaaat gacgaccgcg
     5221 acgcgctgac ccgccaggtc cgctccgcct tcggatttat cggcatcagc gatgtccgca
     5281 tcgcctgggc cgacggccag aacccgcttt tctttaatga cagcgaagtc cgccgcgaaa
     5341 cagcgtttga agccgctgaa gagctggccg aagaactcgc ggaggaaagc gccgcgggtt
     5401 gattccagac ctatgagcgt ttttgttaaa atctgcggaa tctgctccag aagcgacctg
     5461 gagcagattt gtgctttaga gcctgacgcc atcggctttg ttttctggcc gcgcgccaag
     5521 cgctatgtgc gccctgaaca ggtcgccagc tggctgcgaa gcatcccgga gcagattaaa
     5581 aaagtcggcg tttttgtcga accgccgccc gccgaggtcg aagcggtggc gattgcctgc
     5641 caccttgacg taatccaggt gcatctgatt tccaacgatt ggcgcatcga ccggccgctt
     5701 ttccggggac tcgaaacctg gctgtcgccg cgcatgggcg aaggcgtcgg ccgcaatatc
     5761 atacatgccg tcgatcccga accgtcggtt ctgctggctg attcgtttga tccgaacacc
     5821 attggcggaa ccggaaaact cagcagctgg gaacgcgcgc tggcgatgaa aaccgaaacc
     5881 ggcaagccgg tcatgctggc gggcggcctg acgcccgaaa atgtgcgcga cgcaattact
     5941 gcggttcagc cgtggggcgt ggacgtcagt tccggcgtcg aaaaagaacc gggcgtcaaa
     6001 gacatccgca aagtgaaaca gtttatagaa agagtgagac gatgaaaccg gtccagcccg
     6061 attcacaagg ccacttcggc ccgtacggcg gaatttttgt tccggaaacg ctgatcgaac
     6121 cgctccgcga actgacgcgg gtttacaaat cggcgcaaaa agatcccgtc tttaaaaagg
     6181 agcttgagta ctacctccgc gaatacgtcg gccgcccgtc gccgctctgg ttcgcccggc
     6241 ggatgaccgc gcagctcggc ggcccgaaga tttatctcaa gcgcgaagac ctcaaccaca
     6301 ccggcgcgca taaaatcaac aacgtcatcg gacaggcgct gctggcgaaa cggctcggca
     6361 aaacccggct gatcgccgaa accggcgcag gtcagcacgg cgtcgccacc gccaccgccg
     6421 ccgcgctctt cgggatggag tgcaccgtct acatgggcaa ggtggatatg gagcggcagg
     6481 cgctcaatgt tttccgtatg gaaagtctcg gcgcgaaagt cgtcgccgtt accgccggac
     6541 agcagacgct caaagaagcg atcaacgaag cgatgcgcga ctgggtcagc aatctcaaga
     6601 acacgcatta tgtgctcggc accgccttcg gcccgcatcc gtacccgatg atcgtgcgcg
     6661 atttccagag cgtcatcggc aaagaggcgc gtaaacagat tctgaaagcc gaaggacgcc
     6721 tgccgaccgc tatcgttgcc tgcgtcggcg gcggcagtaa tgcgatcggc attttccact
     6781 cctttataaa ggacgaaggc gtccgcctga tcggcgtcga agccggcggg ctcggcattg
     6841 agccgggcaa acacgccgcg cgttttgccg gcggcaaagt cggcgtgctg cagggaacga
     6901 agagttacgt tctgcaggac ggcgacgggc agattctgct gacgcattcc gtcagcgccg
     6961 gacttgacta cgcctccgtc ggtccggagc acgcgatgct gcatgatacc ggccgcgcgg
     7021 aatacactta tattgaagac gccgacgcgg tcgccggatt cgaccggctc gcgcagtggg
     7081 aggggattct gcccgcgctt gaaagctcgc atgccatcgc gtgggtcatt aaaaacgcgc
     7141 acacgtttac aaaagacgac ctgatcatca tcaacgtctc cggccgcggc gacaaagacg
     7201 tccagcagat cgcggaatgg aaaaaggaga atcagcaata aaaggggcgt gccatgtgca
     7261 aaagaagagc gatgaattgc tggatggggt taatcagcgg aatttttatt ctgaatgcaa
     7321 tggctgaaac tctttcgccg ccggaaaaga aggaatattc gaccggtgaa attctggagg
     7381 agcagtttgc gctggcgggc aaaattataa aggtccggtt caaccggata tattatgtga
     7441 gcaaacagcc gaacggaatg tacacgggca atctgcgttc catggtaaca ctctccggca
     7501 attattattc agatcccgcc ggaatacaga tccgattccc gcaggaaggg tttggctata
     7561 tttcgaaatt tctcccgaaa cccggcgttt cttacgagga agaagaaaac agagtctacg
     7621 aatcggattc cgggaccctg tatgttcagg tcagcaacga agcccgtccg ggtttcgttg
     7681 ctatcggcga tcagtaccgc agagaggatg atcaagagga ggaagaatac cgctggtctg
     7741 tgaaaaccga gccgcctgat ctgtccgacc ggcatactgt atcggtcagc gatgtatggc
     7801 ttttccctga gtatctgaac gggagaactg tcgaattgca gtttcgcagc ataggccaga
     7861 tcacacagac agcagtcaat gaataccggg tttccgtttc ggacgggcgg ggatgtttat
     7921 cggttccgct aatagttcct ccggccggcc gggcgttttt taaagaaata gagttgagtc
     7981 agagagagct ggaaggaagc tatcaagtca gccgcgttta tgtatctgtg acaattaatg
     8041 caaacggtac aaccgtgctg acggctcagg gccggcggat gtcaggatcg ggcgacagcg
     8101 cggaataccg gtggtaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcc
     8221 gcgtgttcgg aagatgcgcg ggtaacagtc gaactgagtt cggtaaaccg gaaacagctc
     8281 gacctggttc tgcggctgcc gccgcagatc gccgcgctgg aatcgcgcgt tcagaaaatc
     8341 attcaggaac atatttcccg cgggcggatc agcggaaatg tacaggtgga atccgccgcc
     8401 ggcggcgcgc tgacccggat tgatgaaaag cgcgcggcgg aaacggtcgg cagattgcgc
     8461 cgtgccgcga agaaactgaa ccttgcggat gatttgggcg ccagcgtgct gctgcagatt
     8521 ccggggctga tcaaaatgcc gtctgccgaa cagaatccgg aaggaatttt acagacgctc
     8581 gacaaggcgc tgattgccgc gctgaaaaag cttaatgcaa tgcgtatgcg tgaaggcaag
     8641 acgctggaaa tcgatttgct ggcgcgcctc aaactgctcg aacagatgct tgccgttatt
     8701 aaatcccgcg cgccgcaaat tacttccggc taccggaaga aacttttcag cggactggag
     8761 tctgccgggc ttaaaaacct cgcggcggat gagcggatca tcaaagagat cgcgctgttc
     8821 gctgaacgca gcgacatctc ggaagaaatt acacggctgg cgagtcacat tcagcagttc
     8881 aaaaaaattc tgcgcagcgg cgagccggcg ggacggccgc tcgattttct cgcgcaggaa
     8941 tttttccgcg agatcaacac gatcggttcg aaagcgaatg atctgaaaac caccggacag
     9001 gtggtcgcgt tcaaaacaga actcgaacgt atccgggagc agattcagaa tgtcgaataa
     9061 agttacgccg ctgctgctgg tgatctctgc gccgtccggc gcgggtaaat cgtcgctgtg
     9121 cagccggctg gttgaaaaat ttccggggat ggtctattcg atttcctgca cgacacgcgc
     9181 gccgcgcggc agcgaacaga acggcgtgca ttatcacttt ctttccgaag aagaattctg
     9241 cgcgcgcatt gctgccggag aatttctcga gcacgcgcgg gtacacggca accgctacgg
     9301 aacactgaag cagacggtgt atgatgcatt gtcgcagggc cgcgatatca ttatggatat
     9361 tgatgtgcag ggcgcacagc agatccgcgc cgcgtgcgca aagatgcgcg atgatgatgc
     9421 gatcaagcgg agcttcgttg atatcttcat tgcgccgccg tcgatggaag aactgcgccg
     9481 ccgcttgtgc ggacgttcaa ccgacagtgc cgaagtaatt gaacggcgca tgcgtaatgc
     9541 aacggaagaa atgaaacatc aacctctcta tcagcacacg gtcatcaatg atgattttga
     9601 aaaagcagcg gatgaacttt cagcaatcat cgagagcgaa caccacaacc ggtagtaaat
     9661 tttttttgtt tttatacaaa ctctagttgc aagaaaaatt tttaatcgtt atatgttcgt
     9721 tcgtacaggg acaggctgac gtgaagcaga tagagaaata cttccggcag ccggcagagg
     9781 gttaacccaa agtcaaagga gactggaaat ggcagctaag aaagtggcga agaaaaaagt
     9841 agcgaagaag gccgtaaaaa aagtagcgaa gaaagtcgct aaaaaggccg tgaaaaaagt
     9901 agcgaagaaa gtcgctaaaa aagtcgcgaa aaagaaataa cagtttcgac tgattagttc
     9961 tgaacagccc cgggttccgg ggctgttttt ttatatccgt agtttttcgt ttcctttgaa
    10021 aaaatcaccg gctatcctct cgccaattta acagaaatca ggtcgcgcgt atgaaatgtc
    10081 cgaagtgcga acatcgtgaa aacaaggtca tcgacagccg tgaagtgcgg gagggcaccg
    10141 ctatccgccg ccgccgtctc tgcctgaatt gcggacatcg cttcaccacc tatgaagagg
    10201 tgctgcgcgc cgctttgcag gtcattaaac gggatggccg ccgcgaagag ctttcccgcg
    10261 aaaagctgat gcaaagcatt gtggtcgcct gccagaagcg gcccatcagt atccagcaga
    10321 tcgaacagat gacgaacaat gtcatcacgc agagcgaagc cgagtacgaa cgcgaaattc
    10381 cctctactgc gctcggcacc aaagtcatgg ctctgctcga gaaactggac gaagtcgcct
    10441 atgtgcgttt cgcctcggtc taccggcgct tcaaagacgt caatcagttc atgaacgaag
    10501 ctgaaaggct gatcggacgc gaatgatcga tacacaccag agtctacggc tcgatgagcg
    10561 ccgtctttgg tccggatatg tgcagcagct ttttcaaacg ctggatatca gtgaccgcgc
    10621 ctctgccggg gacatcaccg aaagcgtcgc ggttttctgt cagcagcatg cgaatgtttc
    10681 ctcgcacgcg ctttctctgc tgatggcgcg ctcgttttgc atcaccggcg acgcggaagc
    10741 cgccggacgg attctgcgcc atgaccgcga acacagtccg cacgccggat cgtggcttga
    10801 agttctttcc gccggctatc cgtttcctga actctatccg ctgttttctt cgcgcgcgct
    10861 gcgtccgcag agtctgacat cggccggcgc tctgtgggtg ctcgattttg acaaggtgca
    10921 gctgaccgat gcggaccggc acgaactgat tctgtttcag acggtgcgtg tgctgacgga
    10981 aaaagtttcc aatgtgtgga aaaaatcagc cgggcgggga acgcttggcg tcaaaggtct
    11041 tccgcgactg atgcggtttg tccgcggccg ccggatgccg ccgcagcttc cccggcatat
    11101 agaagacgtg ctgacgcggt gcgcccggaa gcacggctgg agccatacgc cggcgattct
    11161 gcttcttgat ttgtgaacaa ccggggctct tctgtgaaaa aacagctttc tatcatagct
    11221 tccatttgta ccggcctgct tctcttcctg agttttccgc cgtttgagat ttcattttgc
    11281 ggatggttcg cgctcattcc gctgattttt gcctgcgccg gcactgcgcc gcgccgcgcc
    11341 gcatttctcg gctggctggc cggcgcggtt ttttttctga gctcgctctt ctggctgcgg
    11401 cacgtgacct ggctgggaac ggccgggctg gcgctgtact gcgcccttta cttcatgccg
    11461 ttcgccgtgt ttgtttcgct tcgccgcgac ggatggcgcg gcacaaacgg atttaaaaat
    11521 atcggctgga tgctgggtgc cgccgccgtc tgggctgccg gggaatatct gcgcgcgact
    11581 gtcatcaccg gctttccgtg gaatccgctc ggtgtcagcc agtacaaaca gcttgcctgc
    11641 attcaaattg cggaatgggg cggtgtttac gcggtctcgg cgctgatggt ttttgtgaac
    11701 gcggcgtttg cggtgacgat tcttcagtac gcgaccggcc tgcgccgcag atcccggatg
    11761 catttggaaa taatgttcgc gctgctgacc gttgcgctgg cctggtctgt cggcatacag
    11821 aaacttttgc acaggccggc cgggggaacc ccggtccgcg ccgctttgat tcagacggac
    11881 gttccggaag tcggaaactg ggaaaaagca gatccggaag tggtctatga aaagctggaa
    11941 cggcttactg cgctcgcaac gcgctcggcg gatcccgatc tcatcatctg gcccgaaacc
    12001 gcgctgcccg actttgtccg tttcagcgaa cgcagcgcag gactggtcag aaagctggtc
    12061 accaacggcg taccgctgct ggccggaagc atggatgcgg tctggcgtgc ggacggaaac
    12121 ccggattatt acaacgcttc gatgcttttc aatacgcgcg gcgagctgct cggcagttat
    12181 tacaaacagc acctcgtgct tttcggcgaa tacattccgt ttgaaggaaa aattccgctg
    12241 atcaatgcgc tgacgccgat cggctccagc ttcagctccg gccgcgcggc gacgcttttc
    12301 aggcggcccg gcgatgaccg cgggttctgc gtgctgatct gcttcgaaga ttctctgccg
    12361 taccttgcgc gcaaggcggc gcgcgccggc gcaacattcc tggttaacca gaccaacgat
    12421 tcgtggtttg atcccgactg cggttccgtt cagcacctcg ccaatgccgt gttccgctgt
    12481 atcgaaaccc gcctgccgat gctgcgctgc gccaataccg gcatcacctg ctcgattgat
//
LOCUS       DAOPZO010000047        12241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_5811_length_12241_cov_9.076974, whole genome shotgun sequence.
ACCESSION   DAOPZO010000047 DAOPZO010000000
VERSION     DAOPZO010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 12241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12241
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5811_length_12241_cov_9.076974"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..1635
                     /gene="purB"
                     /locus_tag="PKI68_04520"
     CDS             280..1635
                     /gene="purB"
                     /locus_tag="PKI68_04520"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF006764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNX52988.1"
                     /translation="MSITALSPLDGRYESKVAELQEAFSEYALVRCRVLVEVLWLKAL
                     CAEKGIPECRALSKAELQQLDAIVAGFTPAEAEKVKAIEKTTNHDVKAVEYYLKEKIA
                     GTSLEELSEFLHFACTSEDINNLSHALMLKQGREILAASQNALVADLAAKAKAWKAVP
                     LLARTHGQTASPTTIGKEFAVFASRLRRAAAVFQTLEIRGKLNGAVGNYNAHLSAYPD
                     VNWPALAKGVIETELGLTQNPFTIQIEPHDYMAELFDALARFNTILLDLDRDIWSYIS
                     FACFKQKTVKGEIGSSTMPHKVNPIDFENSEGNIGLANALLRHMSEKLPVSRLQRDLT
                     DSTVLRNMGVAFGYSLLAYGSTLKGLGKLELNEAKLAADLDNAWEVLAEPIQTVMRKA
                     GIEKPYEKLKELTRGKAIDQAAIHDFIKSLDLADADKARLLEMTPASYIGCAIEIAEE
                     L"
     gene            1635..2129
                     /gene="rfaE2"
                     /locus_tag="PKI68_04525"
     CDS             1635..2129
                     /gene="rfaE2"
                     /locus_tag="PKI68_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNX52989.1"
                     /translation="MRSATAKILTGEQMLAERAKLKAQGRKLVFTNGCFDILHAGHVT
                     YLEFARAQGDCLVLGLNSDASVKRNKGDDRPINSEADRARVIAALECIDYVVLFDEDE
                     PKALIEKIIPDVLVKGEDWAHYVSGREAVEAAGGKVVLAKMVEGRSTTGTIEKVLKVY
                     GGKP"
     gene            2126..3094
                     /gene="rfaD"
                     /locus_tag="PKI68_04530"
     CDS             2126..3094
                     /gene="rfaD"
                     /locus_tag="PKI68_04530"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HNX52990.1"
                     /translation="MSIKYIVTGGAGFIGSNIVKALNARGETDILLVDELGEDEKWKN
                     LVGLQYEDYIDKDDLFDYLEELTLDDVKAVYHLGACSATTELDADYLAYNNYHYTRAL
                     CESCLDAGVRFVYASSAATYGDGTLGYSDADEMTPKYKPLNMYGYSKHMFDLWAIKSG
                     AIREIAGMKYFNVYGPGEAHKGDMRSVVHKSYGQILKDGEVKLFKSHRPGYKDGEQVR
                     DFVYVKDAVEETLWFGEHPEVSGIFNCGTGQVRTWKDLVSAVFCAMGRQPNIQFIDMP
                     EHLRGKYQYHTQADLTKLRAAGYTRPFMSLEDGITDYVQSFLMKEE"
     gene            3100..4128
                     /gene="rlmN"
                     /locus_tag="PKI68_04535"
     CDS             3100..4128
                     /gene="rlmN"
                     /locus_tag="PKI68_04535"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNX52991.1"
                     /translation="MKPAIHGLFLPELEAFCKAQGQPVFRAKQIWDWLYSKRISSADE
                     MQNLPAPFRAQLAEHFSFQCLEKLETKGEPGETQKLLFRLADNELIETVIIPAPRRQT
                     NTVCVSSQVGCRFGCAFCASGQKGVIRNLSAGEIVGEVLEVAKMLGGRPDNVVFMGIG
                     EPFDNYDEVLRAIRILNHPDGLGIGARRITISTCGVVPGIQRLAEEGLQVELSVSLHA
                     PDSETRSRIMPANESWPLDELMDACADYTAKTNRIITFEYTLIKALNDSPAQARQLAN
                     LLRKFSCRVNLIPLSPVEEFDGERPDAETMKIFLETLESAGINTTFRDSKGSSLKAAC
                     GQLRARRL"
     gene            4656..5114
                     /locus_tag="PKI68_04540"
     CDS             4656..5114
                     /locus_tag="PKI68_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52992.1"
                     /translation="MNTEEKVIEELFVGSFHHSLDPKRRLIIPSDWRSMMGETPRLYV
                     FPHPDRKCLYLYTLAEMNRRLRQLRESGGVDAQGEQAVRAMTASADALTVDAQGRVRI
                     KDELLAHVEVKSKVVLVGTLTRIELWSEEHFDMALPAVSALAETVFYGGY"
     gene            5131..5310
                     /locus_tag="PKI68_04545"
     CDS             5131..5310
                     /locus_tag="PKI68_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52993.1"
                     /translation="MNSIAKYMYFSRAIDLTPEKDRYLRLPESGMQRTVLMTPSRAAT
                     FSAACSRRVIGMRLA"
     gene            5321..6250
                     /gene="rsmH"
                     /locus_tag="PKI68_04550"
     CDS             5321..6250
                     /gene="rsmH"
                     /locus_tag="PKI68_04550"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNX52994.1"
                     /translation="MHVPVMLNETLDLLVTNPAGTYIDGTLGRGGHAAEILKRLSPEG
                     RLIGLDRDLEAIEQTKEILKPFGERAIRIHGNFADMKELCRQIGVTEVDGVLLDLGVS
                     SPQLNVAERGFSFAKDGPLDMRMDRTQGRSAADLVNEDDEETLADLIYRFGEERDSRR
                     IARAIVLERQHGRIERTLKLAEIVERAKGGRRGPIHPATQTFQALRMAVNAELESLDA
                     GLEAGLSMLRDGGRMTVITFHSLEDRPVKEFFKRHTVKRESLQQGGEKLIYEEPAVRL
                     LTKKPLTASKQELMNNPRARSAKLRAVEREKAA"
     gene            6247..6600
                     /locus_tag="PKI68_04555"
     CDS             6247..6600
                     /locus_tag="PKI68_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX52995.1"
                     /translation="MKKRQGERSRKAQVRIPFPVVLANILVFVAVFGLSYVWLCARCD
                     TLGQEIKRLETVQRETRRRMINEQDRWSNLLAPANLDRALKRHNLAMHLPDEQQIVRV
                     RNIAGTPSAALAYNK"
     gene            6622..8379
                     /locus_tag="PKI68_04560"
     CDS             6622..8379
                     /locus_tag="PKI68_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNX52996.1"
                     /translation="MKYKGRTIITSVVIATVFIGLGIRLAFLHLRPSPGTLARIESGR
                     HLEMETRGPRGRILDRNGNMLAVDIAAKHVCVDPKFILQYGDVKAVYSALASQFPADR
                     AELWKELNQPDSQYVRVQKFVREEALGPLRNMNLKGVIFEDVPIRDYPKGVLGAHVIG
                     YANYEGVGSAGVEQRMNAQLQGVAGLRISQKDGRRREIYSCRTVDIDPQPGADVYLTI
                     DQQIQHFAEKALDNLMTKYHPKAAWAIVQNVRTGEILAMASLPTFNLNRFSTAPAEWM
                     RNRAINYTYEPGSTMKAMIVASALDCGAVRANDIFDCENGSWYYGGATLHDSHPCGRL
                     TVADIIKKSSNIGAAKVALQMGDAKVYKCLRSFGFGDRTGLELPGEDAGILWKTDRWS
                     KMSITRIAMGHEVLVTSMQMLSAIGAIANDGIRLRPLIIRKVVNSKGETVLAPQPEVL
                     ARPIRPETAKEMRRLMARVTQPGGTATAAALPGYLVGGKTGTAQKVDLEHGGYYHDKF
                     MASFVGFLPVENPTISIIVVADEPQQGGYYGGTVCAPYFREIADQTVRYLRIPPEGFT
                     TEYVTEIETGGSGEESISD"
     gene            8387..9829
                     /locus_tag="PKI68_04565"
     CDS             8387..9829
                     /locus_tag="PKI68_04565"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNX52997.1"
                     /translation="MTLKELLDGIETVEVAGDTSGEITGIVYDSRRAVPGSLFVALHG
                     AKADGAAYIEDASQRGAAAVVSQSPCAFGGNFPCVQVADARLALAQLAAAFYKNPAAR
                     LCTVGITGTNGKTTVSFMLREIFKAAGRRPGLLGTVRYEIGDRVIPASRTTPESLDLQ
                     AMFAQMERAGCDSAVMEVSSHALAQHRVDGVLYDAAIFTNLTQDHLDYHGTLEEYFIV
                     KSRLFNQVRRFAVINCDDPWGKRLLNEKQTLKGLLSYGFEEGAAVRGLDPVTDTNGSR
                     MRVQSPWGEAEITLQLIGRFNLYNALAAFAAAAALGIPLPVIVKALSEMQCVPGRLEA
                     VPNNKGKRVFVDYAHTDDALRNVLETLREITSGKLVVVFGCGGNRDKGKRPLMGKVAA
                     RIADYSIITNDNPRNEIPEKIAADIAGGFDSERKYEIVLDRKAAIARGLELVGRKDIL
                     LVAGKGHETCQEFNGAIFPFDDREAVREAL"
     gene            9829..11094
                     /locus_tag="PKI68_04570"
     CDS             9829..11094
                     /locus_tag="PKI68_04570"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNX52998.1"
                     /translation="MEKIQFSAAECAGWVSGAWRNSDKNFPILGISHDTRTLKPGELY
                     IAIKGASHDGHDFVRQAVEKGATGLIVEREFPAYGSVPQLIVPDTMPALRALAAGVRR
                     HWTGTVIGITGSVGKTTVKELIASVLAQKGTVCKTPGNWNNDIGLPLSMLAADRNADF
                     FVFELGMNHPGEIDQLAGLLCPDWAVLTDIGKAHIEFFESLEKIAEEKASLLAYADNA
                     LLDETSEWFALFKSKCKGRIVTFGKEPFAFTAPLPGEHMVRNSRRAAALGLALGLSPE
                     AVQSGLSAFRPAPMRWERSMHNGIVFINDAYNANPLSMRASLTTFSEMPCEGRRFAVL
                     GGMRELGGTAEAEHRELGRFVDTLKLDSVLTIGEAGAQMACAGITGIDKPAAVDFLRT
                     HLRAGDMVLFKASRGERLETILEDLKTRI"
     gene            11110..>12241
                     /gene="mraY"
                     /locus_tag="PKI68_04575"
     CDS             11110..>12241
                     /gene="mraY"
                     /locus_tag="PKI68_04575"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNX52999.1"
                     /translation="MFYWLYQFNEVISSLRIFRYITFRAVMAAGTAFIFSLIVGPWLI
                     EKLRAINFGEQKTDERVAGLAQDKRAKVGTPTMGGLMIIFTTVSATVLWAEPGNFFML
                     CALTTFCFLGVLGFIDDYAKIKKSKGLSPRAKLAAQAGWTILLLAALCANPETCLRTQ
                     QLMVPFIKYPVISSMGLFGTLAFIFLVLVGSSNAVNLTDGLDGLAIGCTNSAAAAYLV
                     MAYAAGNFIFAEYLQVPFVKGGGELAVFCGALLGAGLGFLWFNCHPARVFMGDTGSLA
                     IGGGIAAVAILIKQELVLVIVGGVFVMEAASVLIQQSWFKVTKRIYGEGRRVFRCAPL
                     HHHFEFIARDRAAAAGRSVGAAENMITIRFWILSIIFALIGVA"
BASE COUNT         2675 a         3305 c         3630 g         2631 t
ORIGIN      
        1 gcggcgaacg cggttgtgcc ggaggccgtc agcaatcctg cgagaccggt tgctgtaacg
       61 gaacggattc ttctttttga tcggatcata acggagcact ccttttgagt cggtccagcc
      121 tgcatttacc ttgcgtctcc cggcagtccg gaacgcaatg cggccgtaag caatagatga
      181 tagcgaaacc gccgcaggcc gacaaatccc aaaccttgga aatagcgttg tcatcaaggc
      241 gcgggcggga tattttcgga gcctttgaag gagaagctta tgtcgattac cgcattgagt
      301 ccgcttgacg ggcgttatga atctaaagtg gccgaactgc aggaggcctt ttccgaatac
      361 gcgctggtcc gctgccgggt gctggtggaa gtactctggc tcaaagcgct ctgcgccgaa
      421 aagggaatcc cggaatgccg cgccctgtca aaagctgaac ttcagcagct cgacgcgatt
      481 gttgcgggct tcactccggc cgaggccgag aaggtcaagg cgattgagaa aaccaccaac
      541 cacgacgtca aggcggttga atattatctg aaagagaaga tcgccggaac gtcactggaa
      601 gagctgagcg aatttctgca cttcgcctgc acctcggaag atatcaataa cctgtcgcac
      661 gcgctgatgc tcaaacaggg tcgcgaaatt ctggcggcgt cgcagaacgc gctggtggcg
      721 gatctggccg ctaaagccaa ggcgtggaaa gcggttccgc tgctggcccg cactcacggc
      781 cagaccgctt cgccgaccac catcggcaaa gaatttgcgg tattcgccag ccgcctgcgc
      841 cgcgccgccg cagtttttca gaccttggaa atccgcggta agctgaacgg cgcggtcggt
      901 aactacaacg cgcacctttc ggcgtatccg gatgtgaact ggcccgcgct ggctaagggt
      961 gtcatcgaga ccgaactcgg gctgacgcaa aatccgttca ccattcagat tgagccgcac
     1021 gattatatgg cggaactgtt cgacgcgctg gcgcgcttca ataccatcct gctcgacctc
     1081 gaccgcgaca tctggagcta catttccttt gcctgcttca agcagaaaac cgtcaaaggc
     1141 gagatcggtt cctcgaccat gccgcacaag gtcaatccga tcgacttcga aaactccgaa
     1201 ggcaacatcg gcctcgccaa cgcactgctg cggcatatgt ccgaaaaact gccggtttcg
     1261 cgccttcagc gcgacctgac cgactcgacc gttctgcgca atatgggcgt tgcgttcggc
     1321 tattcgctgc tcgcctacgg ctcgacgctt aaaggtctcg gcaagctgga gctgaatgaa
     1381 gcgaagctcg ccgccgatct cgacaatgcc tgggaagtgc tggccgagcc gatccagacg
     1441 gtgatgcgca aagcgggcat cgaaaagccc tacgaaaagc tgaaagagct gacgcgcggc
     1501 aaggcgatcg atcaggcggc cattcatgac ttcattaaga gtctcgatct ggccgatgcc
     1561 gataaagccc gtctgcttga aatgacaccg gcgagttata tcggttgtgc aatcgagatt
     1621 gcggaagaac tctaatgaga agtgcaacgg ccaaaattct gaccggcgag cagatgctcg
     1681 ctgagcgtgc gaagctcaaa gcgcaggggc gcaagctggt ttttaccaac ggctgcttcg
     1741 atattctgca cgccgggcat gtcacctatc ttgaatttgc ccgcgctcag ggcgactgtc
     1801 tcgtgctcgg tctcaactcc gatgcgtcgg tcaaacgcaa taaaggcgac gaccggccga
     1861 tcaacagcga ggccgaccgg gcgcgcgtga tcgccgcgct ggagtgcatc gactatgtcg
     1921 tgctgtttga cgaagacgaa ccgaaggcgc tgattgaaaa aattattccg gacgtgctgg
     1981 tgaaaggtga ggactgggcg cattacgtca gcgggcgcga agcggtcgaa gccgcgggcg
     2041 gcaaagtggt gctggccaaa atggttgaag ggcgttccac caccggcacg attgaaaaag
     2101 ttctgaaagt ctacggaggt aagccgtgag cattaaatac atcgtcaccg gcggagccgg
     2161 ctttatcggc agtaatatcg tcaaggcgct gaatgcgcgc ggcgaaaccg atatcctgct
     2221 cgttgacgaa ctgggcgagg acgaaaaatg gaaaaacctc gtcggcctgc aatacgagga
     2281 ctacatcgac aaagacgatc tctttgatta tctggaagag ctcacgctgg atgacgtcaa
     2341 agccgtttat catctcggcg catgcagtgc gacgaccgag ctggatgccg attatctggc
     2401 ctataacaac taccactaca cccgcgctct gtgcgaaagc tgtctggatg ccggagtccg
     2461 cttcgtttac gcctccagcg ccgcaaccta cggcgacggc actctcggtt attccgatgc
     2521 cgacgaaatg acgccgaagt acaaaccgct gaacatgtac ggctattcca agcacatgtt
     2581 tgatctctgg gcgatcaaga gcggcgcgat tcgcgaaatc gccggcatga aatacttcaa
     2641 tgtctacggc cccggcgaag cgcacaaagg cgacatgcgt tccgtcgtcc ataaatccta
     2701 cgggcagatt ctgaaagacg gcgaagtgaa gctcttcaag tcgcaccggc ccggctataa
     2761 ggacggcgaa caggtgcgcg acttcgtata tgtcaaagac gcggtcgaag aaacgctctg
     2821 gttcggcgaa cacccggagg tgtccggcat tttcaactgc ggcaccgggc aggtccgcac
     2881 ctggaaagat ctggtcagcg cggtattctg tgcaatggga cgccaaccga atattcagtt
     2941 catcgatatg ccggaacatc tgcgcggaaa atatcagtac cacacgcagg ccgacctcac
     3001 caaactgcgc gccgccggtt atacacgtcc gtttatgtcg cttgaagacg gcattaccga
     3061 ctatgttcag agcttcctca tgaaagagga ataaccgcag tgaaaccggc gattcacgga
     3121 ctttttcttc cggagctgga agctttctgc aaagcgcagg gacaaccggt tttccgtgca
     3181 aagcaaatat gggactggct ctactccaag cggatttcat ccgccgacga gatgcagaac
     3241 ctgcccgcgc cgttccgcgc tcagcttgcc gaacacttca gtttccagtg tctggagaag
     3301 ctcgaaacca aaggcgaacc cggcgaaaca cagaaactgc tgttccggct ggcggataac
     3361 gaactgatcg aaactgtgat tattccggca ccgcgccggc agaccaatac ggtttgcgtt
     3421 tccagtcagg tcggttgccg gttcggctgc gctttttgtg ccagcggaca gaagggcgtg
     3481 atccgcaatc tttccgccgg cgagatcgtc ggcgaagtgc tggaagtcgc gaagatgctc
     3541 ggcgggcggc ccgataatgt ggtgttcatg ggaatcggcg aaccgttcga taactacgac
     3601 gaagtgctgc gcgccatccg catcctgaat catccggacg gcctggggat cggcgcccgc
     3661 cgcattacca tcagcacctg cggcgtcgtt cccggtatcc agcgtctggc cgaagagggg
     3721 ttgcaggttg agctttcggt ttctcttcat gcgcccgatt cagaaacccg ctcgcggatc
     3781 atgcctgcca atgaaagctg gccgctcgat gagctgatgg acgcctgtgc cgactatacc
     3841 gctaaaacaa accggattat cacgtttgaa tatacactga tcaaagccct gaacgattcg
     3901 ccggcgcagg cgcgccagct ggcgaatctg cttagaaaat tttcctgtcg cgtgaatctg
     3961 attccgctca gtccggttga agagttcgac ggcgagcgtc cggatgcgga gacgatgaaa
     4021 atctttctgg aaactctgga gagcgccggc atcaatacga cattccgcga ttctaaaggt
     4081 tcttcactta aagccgcctg cggccagctc cgcgcccggc ggctctgatt tgccgaattc
     4141 tgcaaacaaa aaaaccctct tccttgcgga agagggtggt acagggtggg ttgaaaggat
     4201 ggctatttct tcatcgggca gacgccgcca gtgcatccgg cttctttagc cgctttttca
     4261 gcgccgcagg cagctttctt tgcgtcagct tttgctttgc aggctgcgca cattccgccg
     4321 gctgctttgc atgccggaca ggcggcggct ttgcaacttc cgtccgtcgt gcagcaggcc
     4381 tctttgacag cgcaggcttt tgcatcagcg ggagcttttg cgtcagccgg ggtttttgac
     4441 atagcgaaca aggaaccggc gagaacacct attgcgagta cgataaccaa cttcttcatg
     4501 caaacctcca tttgttggtc gaaattctat tctattgctt tcctccggtc aaaccgtcct
     4561 gtacctttct ggctttttta aaaaaatgag aaaattattt gacggaaggt ggtggatggt
     4621 ggcataagat ggagcaagga agggcggagt ggagtatgaa tacagaagaa aaggtgattg
     4681 aagagctgtt cgtcggttcg ttccatcatt cgctggatcc taagcgcaga ctgattattc
     4741 catcggattg gcgatcaatg atgggtgaaa caccccgttt atacgttttt ccgcatcccg
     4801 accgtaaatg tctctatctt tatacgctgg cggaaatgaa ccgccgtctg cggcagttgc
     4861 gtgaatccgg cggagtggat gcgcaaggtg agcaggctgt gcgcgcgatg acggcttcgg
     4921 cggatgctct gacagttgac gcgcagggcc gtgtgcgcat caaggatgag ctgctggcgc
     4981 atgttgaggt aaagagcaag gtggtactgg tcggcacact cacccgcatc gagctctgga
     5041 gcgaagaaca ttttgacatg gcactgcccg ctgtttcagc gctggcggaa accgtttttt
     5101 acggaggcta ttaggaggtc aggtatgagt atgaacagca tagcaaagta tatgtacttt
     5161 tcgcgggcga ttgacctgac gcccgaaaaa gaccgctacc tccggctgcc ggaaagcggg
     5221 atgcagcgca cggtgctgat gacgccgagc cgggcggcta cgttttcggc tgcctgctcg
     5281 cgccgggtaa tcggaatgcg gctggcgtaa tagaggaact atgcatgtac cggtgatgtt
     5341 gaatgagacg ttggatctgc tggtgacaaa tccggcgggc acatatatcg acggaacact
     5401 cggacggggc ggacatgcag cggagatttt gaagaggctt tcgccggaag gacggctgat
     5461 cggactggac cgggatttgg aagcgatcga acagacgaaa gagattttaa aacccttcgg
     5521 ggaacgggcg atacggatac acggaaattt tgcggatatg aaagagcttt gcagacagat
     5581 cggcgtgacg gaggtggacg gggtgctcct cgacctcggc gtcagctcgc cgcagctgaa
     5641 tgtcgcggag cgcgggttca gttttgccaa agacggtccg ctggatatgc gtatggatcg
     5701 tacgcagggc cgttcggccg ccgatttagt gaatgaggat gatgaggaaa cgctggccga
     5761 tctgatttac cgtttcggtg aagagcgcga ctcccgccgg attgcgcggg cgattgtgct
     5821 ggaacgtcag cacggccgga ttgagcggac gctcaagctg gctgaaatcg ttgaacgggc
     5881 caagggcggg cgtcgcggtc ctattcatcc ggccacgcag acgtttcagg cgctgaggat
     5941 ggccgtgaat gccgagctgg aaagtctgga tgccggactg gaagccgggc tttccatgct
     6001 tcgcgacggc ggacggatga cggtgattac atttcacagt ctggaagacc gtccggtgaa
     6061 ggaatttttt aaacgccata cggtgaagcg cgagtcgctt cagcagggcg gcgagaaact
     6121 gatttatgaa gaaccggctg tacggctgct gacaaaaaag ccgctgacgg cctcgaaaca
     6181 ggaattgatg aataatccgc gggcgcgttc agccaaactg cgcgcggttg aaagggagaa
     6241 agcagcatga aaaaaagaca gggagaaaga agtcgcaaag cccaggtacg gattccgttt
     6301 ccggtggtac ttgccaatat tctggtcttt gtcgccgtat tcggactcag ctatgtctgg
     6361 ctgtgcgccc gctgcgatac gcttggacag gagatcaaac ggcttgaaac cgtgcagcgt
     6421 gaaacccgcc gccgcatgat taatgaacag gaccgctggt cgaacctgct ggcgcctgcc
     6481 aatctggatc gcgctctcaa acgccataat ctggcgatgc atctgccgga cgagcagcag
     6541 atcgtccgtg tacgcaacat cgcgggcacc ccgtccgccg ctctcgctta taataaataa
     6601 tgtcccgatg gatctctatg gatgaagtat aaagggagaa cgataataac gtccgtcgtg
     6661 atcgcgacgg tttttatagg gctgggtatt cgcctggcct ttttgcattt acggccctcg
     6721 ccggggacac tggcgcgcat tgaaagcgga cgtcatcttg agatggaaac ccgcggtccg
     6781 cgcggccgca ttcttgatcg caacggcaat atgctggctg tggacatcgc tgccaagcac
     6841 gtctgcgtcg acccgaagtt tattctgcag tacggcgatg taaaagcggt atacagcgca
     6901 ctggcgagcc agtttccggc ggatcgcgcc gagctctgga aagagttgaa ccagccggac
     6961 tcgcaatatg tgcgggttca gaagtttgtg cgcgaagagg ctctggggcc gttgcgtaac
     7021 atgaatctga aaggcgttat ttttgaagac gtgccgattc gcgactatcc caaaggggtt
     7081 ctcggtgcgc acgttatcgg ttatgccaat tacgaaggag tcggcagtgc cggtgttgag
     7141 cagcgcatga atgcccagct gcagggggtt gcgggactgc gcatcagcca gaaagacggc
     7201 cgccgccgcg aaatttacag ctgccgcacg gtggacatcg accctcagcc cggcgcagat
     7261 gtctacttga ccattgatca gcagattcag cactttgccg aaaaagcgct tgataatctg
     7321 atgaccaaat atcaccccaa agcggcgtgg gcgattgtgc agaatgtgcg caccggtgag
     7381 attctggcga tggcttcgct gccgactttt aaccttaacc gcttcagcac cgcgccggct
     7441 gagtggatgc gcaaccgcgc catcaactat acctatgagc ccggttcgac catgaaggcg
     7501 atgatcgtcg cttcggcgct cgactgcggc gctgtacgtg ccaatgacat tttcgactgt
     7561 gaaaacggct cttggtatta cggcggagcc acgcttcatg actcacaccc atgcggcagg
     7621 ctgaccgttg ccgacatcat taaaaaatcg agcaacatcg gtgcggccaa agtggcgctc
     7681 cagatgggtg acgcgaaggt ttataaatgt ctgcggtctt tcggtttcgg cgatcgcacc
     7741 ggtcttgaac tgcccggcga agatgccggt attctctgga aaacggaccg ctggtcgaaa
     7801 atgagtatta cccgtattgc catgggccac gaagtactcg ttacttccat gcagatgctt
     7861 tctgccatcg gcgcgattgc caacgacggc atccgcctgc gtccgctgat tatccgcaag
     7921 gttgtaaatt ctaaaggtga aaccgttctt gcaccgcagc cggaagttct tgcccggccc
     7981 attcgtccgg aaacggcgaa agaaatgcgc cggctgatgg ctcgtgtcac ccagcccggc
     8041 ggaacagcta cagcggctgc gctgccggga tatctggtgg gagggaaaac cggtacggca
     8101 cagaaagtgg atttggaaca cggcggatat tatcacgaca aattcatggc ttcgtttgtc
     8161 ggctttctgc cggttgaaaa tccaaccatc agcatcatcg ttgttgcgga tgaaccgcag
     8221 cagggcggtt actacggcgg aaccgtgtgc gctccgtact tccgtgaaat cgccgaccag
     8281 acggtgcgct acctgcgcat accgcctgaa ggctttacca ccgaatatgt gaccgaaatt
     8341 gaaaccggcg gttccggtga ggaaagcatt tcagactgag gccgctatga cactgaaaga
     8401 actgctggac ggtatagaaa cagtggaagt tgccggtgac acctccggcg aaatcaccgg
     8461 gattgtgtat gactcgcgtc gcgctgtgcc cgggtcgctt tttgtcgcgc tgcacggcgc
     8521 gaaagccgat ggtgccgcgt atatcgaaga cgccagtcag cgcggcgcgg cagccgttgt
     8581 ctcgcagtcg ccctgcgcct tcggcggaaa ctttccctgc gtccaggtcg ccgacgcgcg
     8641 gctggcgctg gcgcagcttg cggccgcatt ttacaaaaat cctgccgcgc gcctctgcac
     8701 ggtcggcatt accggcacga acggaaaaac gacggtcagt tttatgctgc gcgaaatttt
     8761 caaagccgcc ggacggcggc ccgggctgct cggaacggtg cgctatgaaa tcggtgaccg
     8821 tgtgattccg gcttcgcgca cgacgccgga atcccttgac ctgcaagcca tgtttgcaca
     8881 gatggaacgc gccggatgcg acagtgccgt gatggaagtt tcttcgcatg cgctggcgca
     8941 gcaccgcgtt gacggagttc tttacgatgc cgcaattttt acgaacctca ctcaggatca
     9001 tctcgactac cacggcacgc tcgaagaata cttcattgta aaatcccgcc tgttcaatca
     9061 ggtgcggcgg tttgccgtca ttaactgcga cgatccgtgg ggcaaacggc tgctcaacga
     9121 aaagcagacg ttgaaaggac ttttatccta tggattcgaa gaaggggcgg cggtgcgcgg
     9181 actcgatcct gtcacggata cgaacggttc gcggatgcgc gtccagagtc cgtggggaga
     9241 agccgaaatc acgctgcagc tgatcggccg gtttaatctg tacaatgcgc tggccgcctt
     9301 tgccgccgct gccgcgctgg ggattcctct gccggttatc gttaaagcgc tttcggaaat
     9361 gcaatgtgtg cccggtcgtc tcgaagcggt tccgaacaat aaaggcaaac gggtttttgt
     9421 ggattacgcg cacaccgacg acgcgttgcg taacgtgctc gaaaccttgc gcgaaatcac
     9481 atccggaaag cttgtcgtcg ttttcggctg cggcggcaac cgggataaag gcaagcggcc
     9541 tctgatggga aaggttgccg cacgcatcgc cgattactcg atcattacca acgataatcc
     9601 gcgcaatgag attcctgaaa aaatcgctgc ggacattgcc ggcggattcg attccgaacg
     9661 gaaatatgaa atcgtcctcg accggaaagc cgccattgcg cgcggccttg aactggtcgg
     9721 cagaaaagac attctgctgg tcgcgggcaa ggggcatgaa acctgccagg aatttaacgg
     9781 cgcgatattt ccgttcgatg accgcgaagc tgtccgggag gctctctgat ggagaagata
     9841 cagttcagtg cagcggaatg tgccggctgg gtttctggag cctggaggaa ttcagataaa
     9901 aactttccga ttctcggtat ttcccatgac acgcgcacgt tgaagccggg tgaactctat
     9961 attgccatca agggcgcgtc gcatgacgga catgactttg tccggcaggc tgttgaaaaa
    10021 ggcgcgacgg ggctcattgt tgagcgggag tttcctgctt acggaagcgt accgcagctg
    10081 atcgttcctg acacgatgcc ggcgctccgg gcgctggctg ccggcgttcg ccgccactgg
    10141 acgggaacgg tcattgggat caccggcagt gtcgggaaaa ccaccgtcaa agaactgatc
    10201 gcttcggtgc tggcgcaaaa gggaacggtt tgcaaaacgc ccggcaactg gaataacgat
    10261 atcggcctgc cgctcagcat gctggccgcc gaccgcaacg cggacttttt tgtttttgaa
    10321 ctcggcatga accatcccgg cgaaatcgac cagctggccg gcctgctttg tcccgactgg
    10381 gcggttctca ccgacatcgg taaagcgcac attgagtttt ttgagtcgct ggaaaaaatt
    10441 gcggaggaaa aagcttcgct gcttgcctat gccgacaatg cgctgctgga cgaaacatcg
    10501 gaatggttcg cgctgttcaa atccaagtgc aaaggccgca tcgtcacatt tggaaaagaa
    10561 ccctttgcgt ttacagcacc gctgcccggc gaacacatgg ttcgtaactc gcgccgcgcc
    10621 gcggcgctcg gtctggcgct ggggctttcg cccgaagcgg tccagtccgg cctgtcggcg
    10681 ttccggcctg cgccgatgcg ctgggagcgg agtatgcaca acgggattgt ttttattaac
    10741 gacgcctata atgccaatcc gctttcgatg cgcgcttcgc tgaccacgtt ttctgaaatg
    10801 ccgtgcgaag gccgccggtt tgcagtgctc ggcggtatgc gcgaactggg cggaacggca
    10861 gaggccgagc atcgcgagct ggggcggttt gtggatacct tgaagctgga tagcgtactt
    10921 actatcggag aggccggggc gcagatggcc tgcgccggca tcaccggtat cgataagccg
    10981 gccgctgttg atttcctgcg cacccatttg cgcgccggcg atatggtgct ttttaaagca
    11041 tcgcgcggcg agcggcttga aaccattctt gaagatttga aaaccagaat ttaaccggag
    11101 acgtattata tgttttattg gctgtatcag ttcaacgagg tcatctcatc gctgcgtatt
    11161 ttccgctaca tcactttccg cgccgtgatg gccgccggaa ccgcttttat tttcagtctc
    11221 attgtcggtc cctggctgat tgaaaaactg cgcgccatca atttcggtga acagaagacc
    11281 gatgagcggg tggccggcct ggcgcaggat aagcgggcta aagtcggcac gccgacgatg
    11341 ggcgggctga tgattatttt cactacggtc tccgccaccg tgctgtgggc cgagccgggt
    11401 aattttttca tgctctgcgc actgacgacc ttctgctttc ttggcgtact cggatttatt
    11461 gatgactatg ccaagatcaa gaagtccaaa gggctttcgc cgcgcgccaa gcttgccgct
    11521 caggccggct ggacaatcct gctgctggcg gcactttgcg caaatccgga aacctgtctg
    11581 cgcacccagc agctcatggt tccgttcatt aaatatccgg tgatttcttc catgggtctt
    11641 ttcggtacgc tggccttcat ttttctggtg ctggtcggct cttcgaacgc cgtgaatctg
    11701 accgacggac tcgacggact ggcgatcggc tgcaccaact cggcggcggc ggcctatctg
    11761 gtgatggctt atgcggcagg taactttata tttgctgaat acctgcaggt tccgtttgtg
    11821 aagggcggcg gagaactggc ggttttctgc ggcgcgctgc tcggcgcagg gcttggattc
    11881 ctctggttca actgccatcc ggcgcgcgtg tttatgggcg acaccggcag tttggccatc
    11941 ggcggcggta ttgcagcggt cgccattctc attaaacagg aactggtact cgtcatcgtc
    12001 ggcggagtgt ttgttatgga agcggccagc gtgctgattc agcagagctg gtttaaggtc
    12061 acgaaaagaa tatacggcga gggccggcgc gttttccgtt gcgcaccgct gcatcaccat
    12121 ttcgaattca tcgccagaga ccgtgcagct gcagccggac gctccgtcgg cgcggcggaa
    12181 aatatgatta cgatccgctt ctggattctt tccatcatct tcgccctgat cggcgttgcc
    12241 a
//
LOCUS       DAOPZO010000048        11994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_5992_length_11994_cov_8.600553, whole genome shotgun sequence.
ACCESSION   DAOPZO010000048 DAOPZO010000000
VERSION     DAOPZO010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 11994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11994
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5992_length_11994_cov_8.600553"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..690)
                     /locus_tag="PKI68_04580"
     CDS             complement(55..690)
                     /locus_tag="PKI68_04580"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNX53000.1"
                     /translation="MEMLHSFITWIVAVVGHWGYAGIYLLMTLESTFIPIPSEIVMPP
                     AGYLAYKGEMNIYLAVLMGTLGSMTGALINYYLAVFLGRPFILRYGKYVLCPPHKFEK
                     VEKFFLRHGEVGTFTGRLMLGVRHFISVPAGLARMNMRRFLAFTCAGSAIWCGVLAVI
                     GYWIGKVSENLSKEEMYELFKSNGKLAGVIATAVCVMILVSYVLWQRRKKN"
     gene            complement(818..1882)
                     /locus_tag="PKI68_04585"
     CDS             complement(818..1882)
                     /locus_tag="PKI68_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNX53001.1"
                     /translation="MLFLVILVILIALIFEYINGFHDTANSIATTVSTKVLTPRQAVA
                     MATVFNLLGALAGVAVATTIGKGLVDTHFVSIQTIASALIGASIWNLLTWWLGLPSSS
                     SHALVGGLCGATLASANGRWDVIRWSVLNQTSGKMEGVWHKVVLPMFVSPAIGLTVGF
                     LLMGLLMLLLQKVRPRFINMVFGKLQLLSASWMSFGHGTNDAQKTMGIIALILYTATQ
                     AGELDHVPAWLNFLRTPEFTVALWVKLICAFTMAAGTAVGGWRIIKTLGHKMVKLQPI
                     HGFAAQTTAAAIIQVASHFGIPLSTTHIISSSIMGVGATKRLNAVKWNVVSKMVWAWV
                     LTLPVTAILAYGIERLLKLF"
     gene            complement(1899..2528)
                     /locus_tag="PKI68_04590"
     CDS             complement(1899..2528)
                     /locus_tag="PKI68_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNX53002.1"
                     /translation="MNWFKKIVGHDEKFFDLLEASANQAESSVALLVKMLKSTSDIDA
                     LDEMASTRRKDKRITEEITEQLCKTFVTPLDREDIEALSSALYKIPKTVEKFGEKYLV
                     CRPYLESGSFTRQTEILEQTTATVATMVTHLRKRTHLEQIKKENDRLHYLEGEADKLI
                     LQLTHDLYSGKHDPLKVIIHMDLYNILEKVIDRCRDAGNVIFQIVLKYS"
     gene            2658..3908
                     /locus_tag="PKI68_04595"
     CDS             2658..3908
                     /locus_tag="PKI68_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNX53003.1"
                     /translation="MSILIKQVEWNGLTTDVFISGNRIQKIGDCSGQAAEIVLDGRNQ
                     AILPTFHNAHTHAAMAYMRSYADDIELHTWLNDHVWPFESKVTEEDVYNGARLACLEM
                     IKSGTTFFNDMYWFWHGTARAVEESGMRAALSAVFIDFDDPAKAAEQIAQNERLFAER
                     TRYSDRIIFQLGPHAIYTVSEKSLRWCAEFAAKHGLMIHIHLSETQKEVADCIAKHGK
                     RPPEYLDSIGFLGPNVTAAHCIHLDSAEMRLLAERGVKALHCPTSNMKLASGKFRFTD
                     AVNAGMQIAIGTDGAASNNCLDMGAEIKMAALLEKHFTGDTTALNAQTAWQAGTRACA
                     GFYGLNSGVIAEGALADCMLVDLNNERLAPGHHLIADMVYSAGSACIDTVICDGRILM
                     QNRHIEGEDEIIAKGRAFKEKFRR"
     gene            complement(3964..5271)
                     /gene="miaB"
                     /locus_tag="PKI68_04600"
     CDS             complement(3964..5271)
                     /gene="miaB"
                     /locus_tag="PKI68_04600"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNX53004.1"
                     /translation="MTGTYHIWTIGCQMNEADSRYLASQLEALGFAETPRAEHADIAV
                     LNTCVVRQQPEDKAVSKLLSLGDTKKNKRRMIIALMGCMVGKREAEDLANRFPFVDVF
                     MAPSQTDALIDYVLSRDEYSHLDRSQIELADYRLPAVSREKAITAFVPITLGCSHACS
                     YCIIPYRRGPDRSRKPEEILSEVRSLAGQGVKEVTLLGQIVDRYGFDLGDGKNLAWLL
                     KEVAKIDGILRVRFLTSHPSYIDEELIRAVAETEKVCPYFELPFQAGSDRILESMRRG
                     YTNAEYRQTVSDIRKIIPDASINTDVIVGYPTETEEDFQETLKLYGELKLDLAHIAKY
                     SPRPRTISSKMPDDVSEAEKENRANRFDELVAKVMGENNAPMLGTVVEVLAEEFQESK
                     GRWRGRTPQNKLVFFEHDGDCLGQLVKVKLNWTGPFSLIGRAG"
     gene            5413..6231
                     /gene="murI"
                     /locus_tag="PKI68_04605"
     CDS             5413..6231
                     /gene="murI"
                     /locus_tag="PKI68_04605"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNX53005.1"
                     /translation="MKIGIFDSGYGGLSVFKDISARLPEYDYIYLGDNARTPYGNRSF
                     ETIFHFSKQCIDQLFARDCKLIVIACNTASAKALRNLQQKILPVEYPDRKILGIIRPS
                     AEALSGLLDHQEVALWATQGTVDSMSYPLELAKLAPELRLIQQSCPMLVPLVEAGEHD
                     GAGLDYFIKKYWRQTLEKSAGGKISTLLLACTHYPLLYAKIRAIVPDSVKIIEQGNLV
                     ADRLADYLQRHPEVESVLSRKQNRRFLTTDQTERFDRLAEVFLGKSVLSERIEI"
     gene            complement(6316..7284)
                     /locus_tag="PKI68_04610"
     CDS             complement(6316..7284)
                     /locus_tag="PKI68_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNX53006.1"
                     /translation="MRILIVKTSSLGDLFHALPAVHLLKTGLNAEIDWVVNSGYVPLA
                     KCFTDVDRVIPFPRNSLRANLGSFLSDLRKDRYDLVVDMQGLLKSALIARAARSHKRI
                     GPSFHREFAYLFYDHVAAERNKERHAVDENLDVLRFLELPTEPVNFPVAFPPPVFTGQ
                     TSPLKVLLSPCSRHAAKNWPVERFAAVGKALHEKNGAMLYISGVPEDADTCKKLMELL
                     PPGSAHNLCGKTSLVDLGGVLQAMALVITVDSGPMHMAAAAGTPTLAIFGPTDPLRVG
                     PYGPQHRILRKTSVTSYSKDDLQSILGVSAEDAISAAEEMLEQSGR"
     gene            complement(7407..8333)
                     /gene="argF"
                     /locus_tag="PKI68_04615"
     CDS             complement(7407..8333)
                     /gene="argF"
                     /locus_tag="PKI68_04615"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNX53007.1"
                     /translation="MKHLITFKDWSAQEILDVLTLAAKVKAKPQKYADALKQKTLLMI
                     FEKPSLRTRLSFEIGMTQLGGHAIYYDMSTSPMGGGKETIEDSIKVISRMCDIVMARL
                     FHHEDILKMAEHATIPVINALTDESHPCQILADLMTIQEKKGTLKGLKLAYLGDGYNN
                     VTHSLMRGCTKLGVHISVGSPEEQMPAVEVTELCAQYAQESGCELVVTDDPVEAIKDA
                     DIVYTDSWMSYHIPKDEEAARVKLFTPYQVNAALMKHAKPDAIFMNCLPALRGAEQTS
                     EVIDGPQSVVFDEAENRLHAQKAVMLTLCGAV"
     gene            complement(8330..9415)
                     /gene="pyrB"
                     /locus_tag="PKI68_04620"
     CDS             complement(8330..9415)
                     /gene="pyrB"
                     /locus_tag="PKI68_04620"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNX53008.1"
                     /translation="MSRTAVLNQIAWDEFSELNSVAKAPYFQSGGRVYHALVAQQFSR
                     PMLEDLCALATCVRRIAKTREGSAFLQTQLFDKRAMLYFAQPSSRTYLSFYSACQIIG
                     MQTINVRDSSTSSEVKGESPEDSVRTFSSYADLIIMRHPAGGFAERIAWMLSNTHRPV
                     PVVNAGSGKDQHPTQALLDIYTLARSFENIGGIDGKKIAFVGDLKRGRTVRSLAWLLT
                     QYQGVKAYFVAPPELQIGDDILKQLDAAGMPYEISHDFAKVIPEVDAIYMTRIQDEWD
                     VDNESSRINTSSYHFTKDHLKILGKNSVIMHPLPRRLEIDPSVDSDPRAVYWRQVRNG
                     MWIRAALMLNIFGCDGVLRDYCKERKI"
     gene            complement(9595..10284)
                     /locus_tag="PKI68_04625"
     CDS             complement(9595..10284)
                     /locus_tag="PKI68_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53009.1"
                     /translation="MKIRYQGLILLLGLFAASGRADLVNGSVSSPAISDASSMNVSSD
                     LDQGWHAWSASLYDLSENDIWSTWLINQPGCWFGQIFTDNHATYGDVMLKFDVTYMGA
                     NIRSAMFQYALYGTDSTDASTTAFALNTDSNPAGPAWTPVSSGAVSTPDEGLYQAKMT
                     SSGTAYQYLAVRFRFTGMTSGRGTDYATAVDNISVASNHSVIPEPAAVTLIGLGGIVT
                     LFANRLGRRNR"
     gene            complement(10381..11811)
                     /locus_tag="PKI68_04630"
     CDS             complement(10381..11811)
                     /locus_tag="PKI68_04630"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNX53010.1"
                     /translation="MRELPDAPGVYLMRGRTGGIIYVGKAASLRRRVQSYFRESTYHK
                     AEPKLRGLIRSITDFDVLVLRTEAEAVLTEGKLIKEYKPRYNTLFKDDKRFPLLRVDL
                     RKPVPKFELCRIDKNDGAVYFGPYTSGLAARAALEFVERRFGIRQCRPDQPDAETYKH
                     CHNDIIRQCAAPCIGNVTPEEYMERVQTACAFLRGGRPEFIKEVRAQMEAAAEKHRFE
                     EAAALRDMMFLLTRAVKERALVRKTGKMLLDESGTGIRELGEALGLAKPPHVIECFDI
                     SNISGTCAVASMVTAVNGVPVPQRYRRFRIKTVEGSDDPRMMAEAVRRRYSRLKEEGG
                     VFPDLLVVDGGITQLRAARAALAEISVELPSVGLAKQFEEIVRDDQPGLRLPRDSKAL
                     RVMTGLRDEAHRFAISYHRQLRSKRIRESALDEIPTIGQKKKELLLKHFGSFGRLRKA
                     SLDELRTAPGIGPKTASLIHERLNLG"
BASE COUNT         2738 a         3397 c         3249 g         2610 t
ORIGIN      
        1 cagcaccggc agaaccggtg ctgttttcgt tttggatgaa ctccggagcc cggtttaatt
       61 ctttttccgg cgctgccaca aaacatagga gaccagaatc atcacgcaaa ctgcagtagc
      121 aatcacgccg gccagtttgc cgtttgattt aaagagctcg tacatttctt ctttgcttaa
      181 gttttcagag accttgccga tccaataacc gataaccgca agaactccgc accagattgc
      241 agaacctgca caggtaaagg caagaaagcg gcgcatgttc atgcgggcca gaccggccgg
      301 aacggaaatg aagtgacgga ctcccagcat gagacggccg gtaaaggttc cgacttcgcc
      361 gtgccggaga aaaaactttt ccaccttttc aaacttgtgc ggcggacaaa gcacatactt
      421 tccgtaccgg agaatgaacg gacggccgag gaaaacagcc agatagtaat taatcagcgc
      481 gccggtcata ctgccgaggg ttcccatcag aaccgccagg tagatattca tttcgccttt
      541 atacgccagg tatccggccg gcggcatgac gatttcgctg ggaatcggaa taaaggtgct
      601 ttcgagcgtc atgaggagat aaatccccgc atagccccaa tgcccgacaa ccgcaacgat
      661 ccaggtaata aagctgtgca acatttccat tcaaaaactc cggtcggttt caggtgtctg
      721 ggtcaattta attggcgcgg agcatagcgg gtttccaaac ggtttggaat tattcgttta
      781 gaaaatcctg ctttgccgca gtataagcgg taaaacgtca gaaaagtttc agcagccgtt
      841 cgatcccgta cgccagaatg gctgtgacgg gaagtgtcag cacccaggcc cacaccatct
      901 tgctcacaac attccatttg accgcattca accgtttggt ggcgccgacg cccataattg
      961 acgatgaaat gatatgcgtc gtggagagcg gaataccgaa atggctggca acctgtatga
     1021 ttgcagccgc agtggtctgg gctgcaaaac cgtgaatggg ctgcagttta accattttat
     1081 gaccgagcgt tttgatgata cgccagcctc cgaccgctgt gccggcggcc atggtaaagg
     1141 cgcagataag cttaacccac agagccacag taaattcagg tgtccggagg aaattgagcc
     1201 aggccggaac atggtccagc tcaccggcct gggtcgcagt ataaagaatc agagcgatga
     1261 tccccatcgt cttctgtgca tcattggttc cgtgtccgaa actcatccag gaagcgctca
     1321 gcagctgcag tttcccgaac accatgttta taaaccgggg acggactttc tgcaaaagaa
     1381 gcatgagcag tcccatcaga agaaatccaa ccgtaagtcc gattgcggga gagacaaaca
     1441 tcggaagaac cactttgtgc cagaccccct ccattttacc cgaagtctga ttcagaaccg
     1501 accagcggat aacatcccag cgtccgttcg cggaggccag cgtggcaccg cataatccgc
     1561 ccaccagagc atgactggag ctcgacggca accccagcca ccaggtcagc agattccaga
     1621 tactggcgcc gatcagggcg ctggcgatcg tctgaatgga gacaaaatgg gtatcgacca
     1681 gccctttccc gatggttgtg gcaacagcga caccggccag cgcacccagc aggttgaata
     1741 ccgtcgccat cgcaacagcc tggcggggcg taagaacctt agtggatacc gttgtggcaa
     1801 ttgaattcgc cgtatcatga aaaccgttga tatattcaaa tatcagggcg atcaggatta
     1861 ccagaattac taaaaagagc ataagagaac ccgttgcatc atgaatactt caatacaatc
     1921 tggaaaatga cattcccggc atcccggcag cgatcaatca ccttttccag gatgttgtac
     1981 agatccatgt gaataatcac ttttaaggga tcgtgcttcc cgctgtagag gtcatgagtc
     2041 aactgaagaa taagcttatc cgcctcacct tccagataat gcagacggtc attctctttt
     2101 ttaatctgct cgagatgggt gcgtttacgc agatgcgtaa ccatcgtggc gactgtggct
     2161 gtggtttgct ccagaatctc ggtctgccgg gtaaaactcc cgctttccag atacgggcgg
     2221 caaaccagat acttctcgcc gaatttttca accgtttttg gaattttata aagtgcagat
     2281 gaaagcgctt caatgtcctc acggtccagc ggtgtcacaa aggttttaca cagctgttca
     2341 gtgatttctt cggtaatccg tttgtctttg cgacgggtac tggccatttc atcaagcgca
     2401 tcgatatcgg atgtgctttt cagcatcttg accagcagag cgacactgct ttcagcctga
     2461 tttgcactgg cttccagcag atcgaaaaac ttttcatcat gtccgactat ttttttaaac
     2521 cagttcatag ttcacttcta acagaacgcc ccgcagggca cacgaaaaaa aacacgcctg
     2581 aattccgaac ggcctgattt ggggtggtct ttaccgcgtt ttacggctag gctcaaaaca
     2641 cagtagggag cttctgcatg agtattctga tcaaacaggt cgaatggaac gggctgacaa
     2701 ccgatgtttt catcagcgga aaccggattc agaaaatcgg cgattgctcc gggcaggcgg
     2761 ccgaaatcgt ccttgacggc cggaaccagg cgatcctgcc gacttttcat aacgcgcaca
     2821 ctcacgcggc gatggcttat atgcgcagtt atgccgacga cattgaactc cacacctggc
     2881 tcaatgacca cgtctggccg ttcgaaagca aagtgaccga agaagacgtc tataacggcg
     2941 cacggctggc ctgtcttgaa atgatcaaat ccggcaccac tttcttcaat gacatgtact
     3001 ggttctggca cggcaccgcc cgcgccgtcg aagaatccgg aatgcgcgcc gcgctttccg
     3061 ccgtcttcat tgatttcgac gatccggcaa aagcggctga acagatcgct caaaacgaac
     3121 ggctctttgc agaacgaacc cgctacagcg accggatcat cttccagctc ggcccgcatg
     3181 cgatttatac cgtttctgaa aaatcgctcc gctggtgcgc ggaattcgcc gccaaacacg
     3241 gactgatgat tcacatccac ctttccgaaa cccaaaaaga agtggccgac tgcatcgcaa
     3301 agcacggtaa gcgcccgccg gaatacctcg actccatcgg cttcctcgga ccgaatgtaa
     3361 ccgccgcgca ctgcatccat ctggattccg ctgaaatgcg gctgctggcc gaacgcggcg
     3421 tgaaggccct gcactgcccg acatccaaca tgaagctcgc gtccggcaaa ttccgcttca
     3481 ccgacgccgt aaacgccgga atgcagatcg ccattggtac cgacggcgcg gcatcgaaca
     3541 actgcctcga catgggcgcc gaaatcaaaa tggccgccct gctcgaaaag cattttaccg
     3601 gcgacacgac ggcgttgaat gcgcaaaccg catggcaggc cggaacccgc gcctgcgccg
     3661 gattttacgg actcaacagc ggcgtcatcg ccgaaggcgc gctggccgac tgtatgctgg
     3721 ttgacctcaa caacgaacgg ctggctcccg gccatcattt gattgccgat atggtttaca
     3781 gcgcgggcag cgcctgcatc gataccgtca tctgcgacgg cagaatcctg atgcagaacc
     3841 ggcatattga aggcgaagat gaaatcatcg ccaaaggccg cgccttcaaa gaaaagttcc
     3901 gcagataatt atctggcagg gacggatcgc cgggccgtcc gcggcgcttt cggcgaaagc
     3961 accctacccg gcccggccga tcaaggaaaa cggtccggtc cagttcagtt tgaccttcac
     4021 cagctgtccg aggcagtcgc cgtcgtgctc aaagaaaacc aatttgttct gcggcgtacg
     4081 tccgcgccag cggcctttgc tttcctgaaa ctcttcggcc agcacttcga caacggttcc
     4141 gagcatcggc gcgttgtttt cgcccatcac ttttgcaacc agttcgtcga agcggttcgc
     4201 gcggttttcc ttttccgctt cggaaacatc gtcgggcatt tttgatgaga tggtgcgcgg
     4261 ccgcggcgaa tacttggcaa tatgcgccag atcgagtttc agctcgccgt agagcttcag
     4321 ggtttcctga aaatcctctt cggtctcagt cggataaccg acgataacgt cggtgttgat
     4381 cgaagcgtcg ggaatgattt tccggatatc agagaccgtc tgccggtatt cagcattcgt
     4441 ataaccgcgc cgcatcgact cgagaatccg gtcgctcccg gcctgaaacg gcagttcaaa
     4501 gtacggacag actttttccg tttccgccac ggcgcggatc agttcctcgt caatgtacga
     4561 cggatgactc gtcagaaacc ggacgcgcag aatgccgtcg attttcgcaa cctctttgag
     4621 gagccacgcc agattcttgc cgtcgccgag atcgaaaccg tagcggtcga caatctgccc
     4681 gagcagcgtc acctctttga cgccctgccc ggccagcgag cgcacttcgg aaaggatttc
     4741 ttccggcttg cggctgcggt cggggccgcg acggtacgga ataatgcagt aggaacaggc
     4801 gtgcgagcag cccagcgtga tcggcacaaa cgccgtgatc gccttttcgc ggctgaccgc
     4861 cggaagccgg taatcggcca gctcaatctg cgagcggtcg agatgactgt attcatcgcg
     4921 gctcagcaca taatcaatca gcgcgtccgt ctgcgatggc gccataaaaa catccacgaa
     4981 cggaaagcgg ttcgccaggt cttccgcctc gcgcttgccg accatgcagc ccatcagcgc
     5041 aataatcatc cggcgtttgt ttttcttggt gtcgccaagc gacagcagtt tggaaacagc
     5101 cttgtcttcc ggctgctggc gaaccacgca ggtgttgagc acggcgatat cggcatgctc
     5161 cgcccgcggg gtttccgcga aacccagcgc ttcgagctgc gaagcgaggt agcgcgaatc
     5221 ggcctcgttc atttggcagc cgatcgtcca gatatgatag gttccggtca ttcaaaatcc
     5281 ttttgtgaag tgacgctttt accacagcgt ccggctccgg caaagccgca ggaaaggaaa
     5341 aaccgctgac cgcagacatg gaacgatatc acggattgtc acagctcacg gaaaagccca
     5401 tattgcgcgg ctatgaaaat tggaattttt gactccggct acggcggact gtctgttttc
     5461 aaagacatct ccgcccgact cccggaatac gactatatct atctgggcga caatgcgcgt
     5521 acgccgtacg gcaaccgctc ctttgaaacc atcttccatt tcagcaaaca atgcatcgac
     5581 cagctgttcg cccgggactg caaactgatc gtgattgcct gtaacaccgc atcggccaaa
     5641 gccctgcgca accttcagca gaaaatcctg ccggtcgagt accccgaccg gaaaatcctg
     5701 ggcattatcc gcccgtccgc cgaagcgttg tccggactgc tcgaccatca ggaagtcgcc
     5761 ctgtgggcga cacagggaac cgtcgactct atgagttatc cgctcgaact tgccaaactc
     5821 gcgccggagc ttcgcttgat ccagcagtcc tgcccgatgc tggttccgct ggtcgaagcc
     5881 ggagaacacg acggagccgg actcgattac ttcattaaaa aatactggcg gcaaacactg
     5941 gaaaaaagcg ccggcgggaa gatcagcacg ttgctgctgg cctgcacgca ttatcccctg
     6001 ctctacgcaa aaatccgcgc catcgttccg gactcggtga aaataatcga acaaggcaat
     6061 ctcgtcgccg accggctggc cgattacctg caacggcatc cggaagtgga atcggtctta
     6121 agccggaaac agaaccggcg gttcctgaca acggatcaaa ccgaacggtt cgaccggctg
     6181 gcggaagtat ttctcggaaa aagcgtcctc tccgaacgca ttgaaatctg atgcagatgc
     6241 tttgcaggcc gtctttggaa taggacgtaa cggtgaaatg tggcgcaggc attcctgcct
     6301 gtgcaaaagg gcagtttatc ttccgctctg ttccagcatt tcctctgccg cgctgatggc
     6361 gtcttcggct gaaacgccga gaatgctttg caggtcgtct ttggaatagg acgtgacgct
     6421 ggttttacgc aggatgcggt gctgcgggcc gtacgggccg acgcgcagcg gatcggtcgg
     6481 gccgaagatg gcaagcgtcg gcgttccggc ggcggcggcc atgtgcatcg ggccggaatc
     6541 caccgtaatc accagcgcca tggcctgcag gacgccgccg aggtccacca gcgaggtttt
     6601 accgcacagg ttgtgcgccg agccgggagg aagcagttcc atcagtttct tacaggtgtc
     6661 ggcatcttcc ggaacgccgg agatatacag catcgcgccg ttcttttcgt gcagcgcttt
     6721 cccgacagcg gcgaaccgtt cgaccggcca gtttttggcc gcgtgacgcg aacagggaga
     6781 aagcagaact tttaagggtg aagtctgtcc ggtgaatacc ggcggcggaa atgcgaccgg
     6841 gaagttcacc ggttcggttg gaagttcaag gaagcggagg acatcgaggt tttcatcgac
     6901 cgcgtggcgc tccttattcc gttcagcggc gacgtggtca taaaacagat aggcgaactc
     6961 gcggtgaaac gacggcccga tccgcttgtg cgaccgcgcg gcgcgggcga tcagcgcgct
     7021 tttcaaaagt ccctgcatat cgacgacgag gtcgtagcgg tctttccgga gatcagaaag
     7081 aaagcttccc agatttgcgc ggagactgtt gcgcggaaac ggaattaccc gatcaacgtc
     7141 ggtaaagcac ttcgccagcg gcacgtagcc gctgttaacg acccagtcga tctccgcgtt
     7201 cagcccggtt ttcagcaggt gaacggcggg cagcgcatgg aacagatcgc ccagcgagct
     7261 ggttttgaca atcagtatcc gcacaatatc tcctcttacc gaattaaaat ttcacagaag
     7321 gaaacgaagg aaaacaaagg aaaaatccgg ataaaaacag gtttcaacag ccttcgttac
     7381 cctttgttcc cttctgtaaa aactgattat accgcaccgc aaagcgtaag cataaccgcc
     7441 ttctgggcgt gaagccggtt ttcggcttcg tcgaagacaa cggattgcgg gccgtcgatg
     7501 acttcggagg tctgttcggc accgcgcagt gcgggcaggc agttcatgaa gatagcgtcc
     7561 ggtttggcgt gtttcatcag cgcggcgttc acctggtacg gggtgaagag tttaacgcgc
     7621 gcagcttctt cgtctttcgg aatgtgatag ctcatccacg agtcggtata gacgatgtcg
     7681 gcgtctttga tcgcttcaac cggatcgtcg gtgacgacga gttcacagcc cgattcctga
     7741 gcgtactgcg cgcagagttc ggtaacttcg accgcaggca tctgttcttc cggcgagccg
     7801 acgctgatgt gaacgcccag cttggtgcaa ccgcgcatga gtgaatgagt gacgttgttg
     7861 tagccgtcgc cgagataggc cagtttcagt cctttaagcg tgcctttctt ttcctgaatg
     7921 gtcatcaggt cggccagaat ctggcaggga tgactttcgt cggtcagcgc gttgatgacc
     7981 ggaatcgtgg cgtgttcggc cattttcaga atatcttcgt ggtggaacag gcgcgccatg
     8041 acgatatcgc acatgcggct gatgactttg atcgagtctt cgatggtctc tttgccgccg
     8101 cccatcggtg acgtgctcat gtcatagtag atggcgtggc cgccgagctg ggtcatgccg
     8161 atctcaaacg acaggcgggt gcgcaggctc ggcttttcga aaatcatcag cagggttttc
     8221 tgcttcaggg cgtccgcata tttctgcggc ttggctttta ctttggcggc cagagtcaac
     8281 acgtcgagga tttcctgcgc gctccagtct ttgaatgtga tgaggtgctt catatttttc
     8341 tctccttaca atagtcacgc aaaacgccgt cgcagccgaa gatgttcagc atgagcgcgg
     8401 cgcggatcca cataccgttg cgcacctggc gccagtaaac ggcgcgcggg tcgctgtcga
     8461 ccgacgggtc gatttcaaga cggcgcggca gcggatgcat gataacggaa ttttttccaa
     8521 gtatcttcag gtggtctttc gtgaaatggt atgacgaggt gttgatccgg ctgctttcgt
     8581 tgtcaacgtc ccattcgtcc tgaatgcggg tcatgtagat ggcatccact tccggaatca
     8641 ctttggcgaa gtcgtggctg atttcatagg gcatgccggc ggcatcgagc tgtttcagaa
     8701 tatcatcgcc gatctgcagt tccggcggcg cgacgaagta ggctttgacg ccttggtatt
     8761 gcgtgagaag ccacgccagc gaacggacgg tccgtccacg tttcaggtcg ccgacaaaag
     8821 cgattttttt accgtcgatg ccgccgatat tttcaaagct gcgcgccagc gtgtagatgt
     8881 cgagcagcgc ctgggtcgga tgctggtctt taccggaacc ggcgttgacg accggcacgg
     8941 gccggtgcgt attggaaagc atccacgcga tgcgttcggc gaagccgccc gccgggtggc
     9001 gcatgatgat cagatcggcg taggagctga atgtccgcac ggaatcttcc ggcgattccc
     9061 ctttcacctc gctggaggtc gaactgtcgc gcacatttat cgtctgcata ccgatgatct
     9121 ggcaggcgga ataaaatgaa agataggtgc gactggaggg ctgagcaaaa tagagcatgg
     9181 cccgcttgtc aaacagctgc gtctgaagaa acgcgctgcc ttcgcgggtt ttggcgatgc
     9241 gccggacgca ggttgccagc gcgcagaggt cttccagcat cgggcggctg aactgctgcg
     9301 ccaccagcgc gtggtaaacc cgtccgccgg actggaaata aggagccttt gcgacagaat
     9361 tcaattcgct gaactcatcc cacgcaatct gattcaatac tgccgttctg ctcatttatc
     9421 ctccgctttt aaaaccgtgg gaaccttata tgccattttt cccggtcatt ggaaacagga
     9481 agatagagag aatagagcgc cggaataagg tagcgggtat gggagtgcgg aaataaggaa
     9541 aacccgcaaa cccggcagtc acttttttcc ggtcagcccg gaacgcgctt tcatttaacg
     9601 gttccggcgg cccaaccggt tcgcaaaaag cgtaacgata ccgcccagcc cgatcagcgt
     9661 aactgcggcc ggttccggaa taaccgagtg gttcgaggcg acagagatat tatccacagc
     9721 ggttgcataa tccgttccgc gaccgctggt cattccggta aaccggaacc ggacagccag
     9781 atattgatac gccgtgccgc tggacgtcat tttcgcctga tagaggcctt cgtccggagt
     9841 agaaacagcc ccgctgctga ccggtgtcca cgccggaccg gcggggtttg agtctgtatt
     9901 aagagcaaaa gcggtcgtcg aagcatccgt tgaatccgtc ccgtaaagcg catactggaa
     9961 catggcggat cgtatattcg ctcccatata ggtcacgtca aatttcagca tcacgtcccc
    10021 gtaagtcgcg tgattgtcgg taaatatctg tccgaaccag cagccgggct ggttaatcag
    10081 ccaggtggac catatatcat tttcggaaag gtcataaagc gaagccgacc aggcatgcca
    10141 gccctgatct aaatccgaag aaacattcat actggatgcg tcggagatcg ccggactgga
    10201 aaccgaaccg ttaaccaaat ctgcgcgccc ggaagcggca aacagtccca gtaatagaat
    10261 taacccctga tatctgattt tcacaaattc cctgcttgca agtaatgata tttacttttg
    10321 aagtcgaggg acaatacagc acaggaatct gaaaacaatt taatttcaaa gaaattttta
    10381 ttatccaaga tttagtcgtt catgaatgag cgaagctgtt ttcggaccga ttccgggcgc
    10441 ggtgcgcagc tcatccagac tcgctttgcg caggcgcccg aacgagccga aatgtttaag
    10501 caggagctcc tttttctttt gtccgatggt cggaatttca tccagcgccg actcgcggat
    10561 tcgcttcgaa cgcagctggc ggtgatagga aatggcgaac cggtgagcct catcgcgcaa
    10621 gccggtcatc acccgcaacg ctttagagtc gcgcggcagg cgcaggccgg gctgatcatc
    10681 ccggacaatc tcctcaaact gctttgccag cccgacgctt ggaagttcga cactgatttc
    10741 cgccagcgcc gcgcgggcgg cgcgcagttg cgtgattccg ccgtccacta ccagcagatc
    10801 cggaaacacc ccgccctctt ctttcaaccg gctataccgg cggcgcaccg cctcggccat
    10861 catgcgcgga tcgtcactgc cttcaaccgt cttaatgcgg aaccggcggt agcgctgcgg
    10921 caccggcacg ccgttcaccg ccgtcaccat gctcgcgacc gcgcaggtgc cggaaatatt
    10981 ggaaatatcg aaacactcaa taacgtgcgg cggtttcgca agccccagcg cttcgcccag
    11041 ctccctgata cccgttccgg attcatccag cagcatcttg ccggttttgc gcaccagcgc
    11101 ccgctccttc accgcgcggg tcagcagaaa catcatgtcg cgcagcgcgg cggcttcttc
    11161 aaaccggtgt ttttcagcgg cggcttccat ttgcgcacgc acctctttaa taaactccgg
    11221 ccgcccgccg cgcagaaaag cgcaggccgt ctgcacacgc tccatgtatt cttccggcgt
    11281 cacgttgccg atgcacggcg cggcgcactg acggatgatg tcgttgtggc agtgtttata
    11341 ggtttccgca tccggctgat ccgggcggca ctgccggata ccgaaacgcc gttcgacaaa
    11401 ctccagcgcc gcgcgggccg ccagccccga agtgtacggg ccgaaataga cggcgccgtc
    11461 gttcttatca atccggcaga gttcaaactt aggaaccggt ttgcgcagat caacccgcag
    11521 aagcggaaac cgtttatcgt ctttgaacag cgtgttgtag cgcggtttgt attccttgat
    11581 cagcttacct tcggtcagca cagcttcggc ttccgtccgg agcaccagca catcaaaatc
    11641 ggtgattgaa cggatcagcc cgcgcagctt cggctcggct ttatgatagg tgctttcgcg
    11701 gaaatagctt tgaacccgcc tgcggagcga cgccgccttg ccgacataga taatgccgcc
    11761 cgtccgtccg cgcatcagat aaacgcccgg cgcgtccggc agttcccgca acttcttccg
    11821 tatattctcc ggccactgca tttccgaagt ttaccagaac tattccgaaa aacaggatca
    11881 gaaagccgtt ttcggccgag ttttatgttg ccggacacag gccgggtctt tatattccgg
    11941 gctcattttt cagggagttt caacccttaa accaaggagc agtaaatgtc agcg
//
LOCUS       DAOPZO010000049        11966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_6016_length_11966_cov_9.290488, whole genome shotgun sequence.
ACCESSION   DAOPZO010000049 DAOPZO010000000
VERSION     DAOPZO010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 11966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11966
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6016_length_11966_cov_9.290488"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            306..935
                     /locus_tag="PKI68_04635"
     CDS             306..935
                     /locus_tag="PKI68_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53011.1"
                     /translation="MKFRFKYLPVVVAVLLPAGFAPAALPENYPATFSELSALIDGDE
                     LSPVEMMRGGWLAPGYFAPSTEALEFNRQRFAAARTTGEAGLSGLFLAVNGTAAQHQF
                     VCKTLETDQTKRRMMSRLFGTEAAFFQSLENGEPLRPLMNALPSTVRLHSLLRIFSQS
                     KDPLVRRAGLFWGYWFADGDYWQTVRTAAAQEADAVNRACAQRLLRHAR"
     gene            1031..1366
                     /locus_tag="PKI68_04640"
     CDS             1031..1366
                     /locus_tag="PKI68_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53012.1"
                     /translation="MKKWKCEGCGYIHDGDEAPAKCPKCGAPKEQFTLLDEAAAKLVE
                     RSRHTNTLHSHVIDLSRQLEAACNAGIKDELDPGCVDVFTKSRAMAWQIMKLSMTEQQ
                     LHMKKGKWG"
     gene            1497..2444
                     /locus_tag="PKI68_04645"
     CDS             1497..2444
                     /locus_tag="PKI68_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNX53013.1"
                     /translation="MAESNILEVFKALGDEARLRILRAVEIAELSVAEIVEALKMPQS
                     SVSRHLKPLRDSGLLDTRREGTSVYYRRGPVFQDQGFAQLMSEKLAELRGASRDRAAV
                     DRAIEQRRRESAKFFDGIAGRYGSLTQPGGGWQAMAAALAAGFAGQTVADIGCGEGDL
                     TLLLARFAKRVVAVDLSAQMLRVVQERSAEAGVSRRVTVEKGDLEKLPLDTDSMDSAF
                     VSQVLHHAARPGKALEEAARILKSGGRLILLDLAQHDQEWVRSEWADQWLGFDERELR
                     GWLAGAGLKLETFQTLEGPEPAFSVVMIVATKEEKKRSK"
     gene            2447..3865
                     /gene="ahcY"
                     /locus_tag="PKI68_04650"
     CDS             2447..3865
                     /gene="ahcY"
                     /locus_tag="PKI68_04650"
                     /inference="COORDINATES: protein motif:HMM:NF017071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNX53014.1"
                     /translation="MAKKAISKKQDYAVKDLSLAAFGRKEMELAEHEMPGLMALREKY
                     GKDKPLKGANITGSLHMTIQTAMLIETLQILGAKVRWASCNIFSTQDHAAAAIAVTGT
                     PVFAVKGESLEEYWDYTDRILDWGNGKGPNMILDDGGDATLFVHLGLRAESDPSVLDR
                     KPGSHEEKILLAQLKTALKKDPKRFSRIAKGIIGVSEETTTGVHRLYQMMERGELLFP
                     AFNVNDSVTKSKFDNLYGCRHSLVDGIMRATDVMLSGKVAVVAGYGDVGKGSCQSLKG
                     QGARVVVTEIDPICALQASMEGYEVMTMNDACLIGDLFVTTTGCCDVITEAHMRKMKD
                     MAIVCNIGHFDSEIQVEKLKKYKWTNIKPQVDKITFPNKKSILLLAEGRLVNLGCATG
                     HPSFVMSNSFTNQVLAQMELYCNPGKYPVGVYTLPKNLDEEVARLHLEKVGVKLEKLS
                     RAQAKYLGISQSGPFKPEHYRY"
     gene            4064..4246
                     /locus_tag="PKI68_04655"
     CDS             4064..4246
                     /locus_tag="PKI68_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53015.1"
                     /translation="MATNPIGKGTKTIGINMPRGMAEDLEKRADSMHISKSKYCKIVL
                     QQWLQSGKKLTLAEKK"
     gene            complement(4309..5004)
                     /gene="pyrF"
                     /locus_tag="PKI68_04660"
     CDS             complement(4309..5004)
                     /gene="pyrF"
                     /locus_tag="PKI68_04660"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNX53016.1"
                     /translation="MKPALIVALDVSAAREMEEVLSRLPDTIEWYKVGLELFCAEGPS
                     ILDPLKKRKKKIFLDLKLHDIPRTVANAVKTAASHGVNLMTVHAIGGRAMLEAAAEAA
                     RECPNPPKLVAVTTLTSLSQDDFIDLGITRSVSDQALALGKLAISSGIDGLVTSAHEA
                     GVLRRNFPEALLVTPGIRMPDGEIGDQKRVATPAFAVQQGATHLVVGRPILQAKDPAA
                     AADAIFADIKSAP"
     gene            5076..5546
                     /locus_tag="PKI68_04665"
     CDS             5076..5546
                     /locus_tag="PKI68_04665"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HNX53017.1"
                     /translation="MQQEIERKFLVVGDGWRVRAGAGKICCQGYITSGPDGATVRARL
                     LGEKGFLTIKGPTANISRPEFEYEIPAADAEYIIENLCGDRLVSKKRYTLEAGGMTWE
                     IDEFSGLNEGLIVAEIELQSEDQSFSKPAWLGAEVTFDPRYTNAVLARHPFTRW"
     gene            5640..6248
                     /locus_tag="PKI68_04670"
     CDS             5640..6248
                     /locus_tag="PKI68_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNX53018.1"
                     /translation="MKKAVIILFAGMVLNSGYAELFTFNMINKNGESWDGFSVGVYTN
                     TGGLTLEASLSAYLNGNYSSGSQLNSTVSEFGINAAGSGDDTALFDTNNGQEALWVSF
                     NRAVTIKSITVSSFTGGNVETGAYQVADGSLVNFTASGIYTVDSILSQGSYFKVIAVN
                     EGGGNGWSLNSFTVEAVPEPATVAILSFGGLAACLLRRVLFS"
     gene            6345..6758
                     /locus_tag="PKI68_04675"
     CDS             6345..6758
                     /locus_tag="PKI68_04675"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNX53019.1"
                     /translation="MNIFEKIVNREIPATIVYEDSDTLAFMDIGPVIKGHTLVIPKTC
                     YDPVTETPDEVVAKLMLVAKRIAAAQIKGLGADGVNIIQNNGKAAGQVVPHIHFHVIP
                     RFEGDGHHWNWAAKKYDSPAEMEKLAGQIREGLAG"
     gene            6755..7141
                     /locus_tag="PKI68_04680"
     CDS             6755..7141
                     /locus_tag="PKI68_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53020.1"
                     /translation="MKHPNAEELLQGILDRDSRYTVEAYTFVRAGLDYTVRRLETPRH
                     VSGQELLDGIREFALAEFGPMTKTVLNRWGIMRTEDVGEIVFNMVETGLLGKTDKDSR
                     ADFANGYDFDEAFRKPFQPRSANRKA"
     gene            complement(7138..7437)
                     /locus_tag="PKI68_04685"
     CDS             complement(7138..7437)
                     /locus_tag="PKI68_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNX53021.1"
                     /translation="MNKQSTPYLCHIFICSNIRENHPANPGCGTKGGGALKSKLKAAV
                     KERGWTGKVRVSTTGCLGLCTKGPNILLHPQGTWFSGVTEADIDSILETAGNFIS"
     gene            complement(7434..>7838)
                     /locus_tag="PKI68_04690"
     CDS             complement(7434..>7838)
                     /locus_tag="PKI68_04690"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNX53022.1"
                     /translation="PITLTLDGDGKAVELNLPPLKTEPCRKFAFLDNAVDFQMVEKMF
                     PKSSVLGSTPFQQAVWDALEKIPRGQTRTYGEIAAAIGRPKAVRAVGTACGANPLPVF
                     IPCHRVTAKNGLGGFGSGLPWKVLLLKSEGAV"
     assembly_gap    7839..7938
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7939..9241)
                     /locus_tag="PKI68_04695"
     CDS             complement(<7939..9241)
                     /locus_tag="PKI68_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53023.1"
                     /translation="MSRGPVHEAFAEPVTLQTQAGLIVPQQPPANIQEVPPAEKPQGS
                     QYVWIPGYWSWDADRSGYIWVSACWRAAPPNMSWVPGYWMQVSGGWEWVAGYWAAANT
                     REIVYLPAPPTVEDLEPVTLQPSPDMIWVPPCMYWSEGRYIRRAGYWLTAQPNWVWVP
                     SHYIMTPRGYIFAEGHWDYALERRGVLFAPVYFPPAVYGRVGFTFSPSIVIDVGLLQV
                     SLFAYPRYCHYYFGDYYDSVYLNIGIFPWFECRRYHTWYDPIYEHDRWRHHAEPRWEE
                     HERNEYRRRCDDRNLRPAHTFREQESRLSRLPEPQRQASQLVQPIASVASRKTPMKYE
                     RIDADARQKISQQSTAVHTYSDNRNRWESPAATSRTTQPTAEHRTTTGTPPIERKQTV
                     RPVEERKTAVSPTTQTVQPDQQLRPVERRQTVQPAEEHKTDA"
     gene            complement(9518..10252)
                     /locus_tag="PKI68_04700"
     CDS             complement(9518..10252)
                     /locus_tag="PKI68_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HNX53024.1"
                     /translation="MKKFFFIIACALLLAGCGQKSGAQLDTADEKDSHVRKGLELLQQ
                     KNWDAAIGQFETALAKKPELGRPDLELAMIYHQHEKNYARAIYHYERYLEKRPLTEKR
                     ALIQDWIRQAKISLSGEAGISGGDVSEELVRLTRENNMLRKQLETAGGAAPAAVANVK
                     TLLTQPPAARPATAPAAAPVAVKAADPVKAARTYQVRPGDTLGRIAGSVYGDTGKWKK
                     IYDANRDKMKSETDIKVGQTLTIPGL"
     gene            complement(10255..11298)
                     /locus_tag="PKI68_04705"
     CDS             complement(10255..11298)
                     /locus_tag="PKI68_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX53025.1"
                     /translation="MNKKVLVTGGSGFLGINMIRYLLRQGVTDITVLDLVEFDYPERD
                     RINAVVGDIRNAGTVREVMKGVNWVVHTAAALPLYTKEEIFSTDIEGTRILLAAAEKE
                     KVERFVHISSTAVYGIPDHHPLFETDPMIGVGPYGHAKIEAEKVCLEYRAKGMCVPII
                     RPKSFVGPERLGVFALFYDWAKDGKNFPMIGSGNNRYQLLAVEDLCEAIWLCLTKDEA
                     AVNDIFNVGATDFTTMKEDYQAVLDRAGFGKKVIGTPARPLIWALRILEFFKLSPLYK
                     WVYETACEDSFVSTDKIAQKLNFHPKFSNKQALVCNYEWYLANLHTFEGKGGISHRVP
                     WSQGILKFFKLFF"
     gene            11328..>11966
                     /gene="thiL"
                     /locus_tag="PKI68_04710"
     CDS             11328..>11966
                     /gene="thiL"
                     /locus_tag="PKI68_04710"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNX53026.1"
                     /translation="MKTLKDVGEHNAIKALTANLKAVGDDCAVLPLDANYDLVLTTDP
                     LISGVHFTPGTDPGQIGWKAAARVLSDFAAMGADPQYILINLVAPPEQDFQTLEKIYA
                     GVSRIRELFGVDLVGGDLAQGPTLELHVFGVGRVPKGKALLRSGARPGDVIYVTGPLG
                     GSFESGKHLTFMPRIKEAKWLRQSGVVRSMMDISDGLATDLRHILKASKVGAA"
BASE COUNT         2597 a         3166 c         3499 g         2604 t
ORIGIN      
        1 tgcaggtggc ggtcgatgta gattctggag cggtcaacgc tgtagccggc gaggtacgga
       61 atgtcgtgat cgcggtcgag ctggacgcgc ttaaggattt ccgccacgga cttgatcagc
      121 atctgcgaac tgaccaggcc gtcgttggtg gtaagctggt tgtggtggcc gctcataact
      181 tgtctccccg ttcggttttg gccggagact acagcgcgcg gtcagccgtg gccacttctt
      241 tccgttctgg cggggcgtcc gccgaactcg acttgacgga gggaatgcgg cattttatgc
      301 ggcgtatgaa gtttcggttt aaatacctgc cggtggtggt cgcggttctt ctgccagccg
      361 gcttcgcgcc ggcggctctg ccggaaaatt atccggccac gttcagcgaa ttatccgcgc
      421 tgatcgacgg cgacgagctc tctccggtgg agatgatgcg cggcggctgg ctggctcccg
      481 gttattttgc gccgtccacg gaggcgctgg agttcaaccg gcagcgtttc gcggctgccc
      541 gtacgacggg cgaagccgga ctttccggcc tgtttctggc ggtaaacggc acggcggcgc
      601 agcatcagtt tgtttgtaaa acgctggaaa cggatcagac gaagcggcgg atgatgagcc
      661 ggctcttcgg taccgaggcg gcctttttcc aatcgctgga aaacggcgaa ccgttgcgtc
      721 cgctgatgaa tgcgctgccg tctaccgtcc ggctgcattc gctgctgcgg atcttcagcc
      781 aatccaaaga tccgctggtc cgccgcgcgg ggctgttctg gggctattgg tttgcggacg
      841 gcgactattg gcagacggtc cgaaccgccg cggcgcagga ggccgatgcg gtcaaccggg
      901 cctgcgccca gcggctgctg cggcacgcgc gctaaagccg accaaatcac ttggattaag
      961 acattgagtt tcgcggcgca gagggtatgc ttcgaggcat tacaaggaaa cccataaggg
     1021 aggaagagtc atgaagaaat ggaaatgcga agggtgcgga tatattcacg atggcgacga
     1081 agctccggcc aagtgtccga aatgcggcgc gccgaaggaa cagttcacgc tgctggatga
     1141 agcggcggcg aaactggttg agcgctcgcg gcataccaat acgctgcatt cgcacgtcat
     1201 cgatttgtcg cgtcagcttg aagccgcctg caacgcgggc atcaaagacg aactggatcc
     1261 gggctgtgtt gatgtattca caaaatcccg cgctatggcg tggcagatca tgaagctttc
     1321 catgaccgaa cagcagctgc acatgaaaaa aggcaagtgg ggctgagaag acgaaaggct
     1381 ataggcttta gaccttagac ttagtggaag gcgcaccgga aaacggggcg cctttttttc
     1441 tgtcttgctt ccggccggta aatatccttt catccggaaa agcggatgga gtgaatatgg
     1501 cggagagcaa tattcttgag gttttcaaag cgctgggcga cgaagcccgg ttacggattc
     1561 ttcgtgccgt ggaaattgcc gagctgtcgg tagccgaaat cgtggaagcg ctcaaaatgc
     1621 cgcagtcgtc ggtgagccgg catctgaagc cgctgcgcga cagcggactg ctcgatacgc
     1681 ggcgcgaagg cacgtcggtc tactaccggc gcgggccggt tttccaggat caggggtttg
     1741 cccagctgat gagcgagaag ctggcggaac tgcgcggcgc aagccgcgac cgcgcggcag
     1801 tcgaccgtgc gattgaacag cgccgccggg agagtgcgaa attttttgac gggatcgccg
     1861 gtcgctacgg ctcgctgacg cagccgggcg gcggctggca ggcgatggct gcggctcttg
     1921 ccgccggatt cgccgggcag acggtggccg atatcggctg cggcgaaggc gatctgacgc
     1981 tgctgctggc gcgttttgca aaacgggtcg tggcggttga tctgtctgcg cagatgctgc
     2041 gcgtggtgca ggagcgttcc gcggaagcag gtgtgtcgcg gcgcgtgaca gtggaaaaag
     2101 gtgatctgga aaaactgccg ctggataccg acagcatgga ttcagccttt gtgagccagg
     2161 tgctgcatca tgcggcgcgg ccgggaaagg cgctggagga agcggcgcgt attttgaaat
     2221 ccggagggcg gctgattctg ctggatctgg ctcagcatga tcaggagtgg gtgcgcagtg
     2281 agtgggcgga tcagtggctc gggtttgatg agcgtgagct gcggggctgg ctggccgggg
     2341 ccgggttgaa gctggagact tttcagacgt tggaggggcc ggagccggct ttttcggtag
     2401 tgatgattgt tgcaacaaaa gaagagaaga aacggagtaa atagatatgg cgaagaaagc
     2461 gatttcgaag aaacaggatt atgcggttaa agacctttct ctggcggcat tcggacgaaa
     2521 ggaaatggag ctggccgagc acgaaatgcc cggtctgatg gcgctgcgcg aaaaatacgg
     2581 caaggataag ccgcttaaag gcgcaaacat caccggatcg ctccatatga cgattcagac
     2641 ggcgatgctg attgaaacct tgcagattct cggcgcaaag gtccgctggg ccagctgcaa
     2701 tattttttca acacaggatc acgcggccgc tgcaattgcc gtgaccggta cgccggtgtt
     2761 tgccgtgaaa ggcgagtcgc tggaagaata ctgggactac acggaccgca ttcttgactg
     2821 gggtaacggc aaaggcccga acatgattct cgacgacggc ggcgatgcga cgctgtttgt
     2881 acatctcggg ctgagagcgg aaagcgatcc ttccgttctg gatcgcaagc cgggcagcca
     2941 tgaggaaaaa attctgctgg ctcagctgaa aaccgcgctg aagaaagatc cgaaacggtt
     3001 ttcgcgcatc gccaaaggca ttatcggcgt cagcgaagaa accaccacag gcgttcaccg
     3061 gctctaccag atgatggaac gcggcgagct gctcttcccg gcgttcaatg tgaatgattc
     3121 agtgaccaag tcgaagtttg ataatctcta cggatgccgc cactcgctgg tggacggtat
     3181 tatgcgcgcc accgacgtca tgctttcagg caaggtcgct gtggtcgccg gttacggtga
     3241 tgtgggtaaa ggctcctgcc agtcgctcaa aggacagggc gcgcgggttg tcgttacgga
     3301 aatcgatcct atctgcgcgt tgcaggcttc gatggaaggc tatgaagtga tgacgatgaa
     3361 tgatgcctgt ctgatcggcg acctgtttgt caccacgacc ggatgctgcg acgtcattac
     3421 agaagcgcac atgcgtaaga tgaaagacat ggccattgtc tgcaatatcg gccatttcga
     3481 cagcgaaatt caggttgaga agctgaagaa atataaatgg accaacatca aaccgcaggt
     3541 cgacaagatc accttcccga acaaaaagag tatccttttg ctggctgaag gccgtctggt
     3601 gaacctcggt tgcgccacgg gccatccgag ttttgtgatg tctaacagct ttaccaatca
     3661 ggttctggct cagatggagc tctactgcaa ccccggtaag tatccggtcg gtgtttacac
     3721 cttgccgaag aatctcgacg aggaggtagc gcggctgcac ctggaaaaag tcggcgtgaa
     3781 acttgagaag ctttcccgcg cccaggctaa atatcttggt atttcgcagt ccgggccttt
     3841 caagccggag cattaccgtt attaaccgga gatgaacctc cgcccgtaga cgggcggagg
     3901 cttctattca gcatcaaacg acagtctctt tggcggacag accacaaaga gattaaatag
     3961 ccggttcggc aggtcatctt ttcgtctgtt tcgcccggtg tttcagtttg ctttttctaa
     4021 gattttatta taatagttaa acaatagaaa aggaggcagg tgtatggcaa caaaccccat
     4081 tggaaaagga accaagacca tcggtattaa tatgccgagg ggaatggcgg aagatttgga
     4141 aaagcgtgca gactcaatgc atatttccaa gagcaagtac tgcaagattg ttcttcagca
     4201 gtggctccag tccggcaaga aattgacctt ggctgaaaag aagtagggaa atttctccgg
     4261 acttcgacgg atattttctc tgaaaatatt acgaaaagcg ctccgtaatc acggagcgct
     4321 ttttatgtcg gcaaagatcg cgtctgctgc ggctgcagga tctttagctt gcagaatcgg
     4381 gcggcctacg accagatgag tggctccctg ctgtacggca aaagcaggtg tcgccacgcg
     4441 tttttggtcg ccgatctcgc cgtcgggcat acgaattccc ggtgttacca gtaaagcctc
     4501 cggaaaattc cggcgtaaca cgccggcttc atgggcgctg gtcaccaaac catcgattcc
     4561 tgaactgatc gccagtttcc cgagcgccag ggcctgatcg gatacgctgc gggtgatgcc
     4621 cagatcaata aaatcatcct gagacaggct ggtcagcgta gtgacggcca ccagcttcgg
     4681 cggattaggg cactcgcgcg ccgcttcggc ggcagcctcc agcattgccc ggccgccgat
     4741 ggcatgaacg gtcatcagat ttacaccgtg gctggcggcg gttttcacag cgttggcaac
     4801 ggtgcggggg atatcgtgca gcttgagatc caggaatatt tttttcttcc tttttttcaa
     4861 cggatcgaga atggacgggc cttcagcgca gaagagttca aggcctactt tataccattc
     4921 aatagtatcc ggcagccggc tgagaacctc ttccatctcc cgtgcggctg atacatccag
     4981 tgcaacaatc aaagcaggtt tcattttatt cctttcctta aaatctggtt ccgctaaaaa
     5041 tcaccaaaga acggccggaa agcaaggatt agttcatgca gcaggaaatc gaacgcaaat
     5101 ttctagtggt cggcgatggc tggcgtgtcc gtgccggagc cggaaaaatc tgctgtcagg
     5161 gttatataac ctcagggccg gacggcgcca cggttcgcgc ccgcctgctg ggagaaaagg
     5221 ggtttttgac cattaaagga ccgacggcga acatatcccg acctgaattc gaatatgaaa
     5281 ttcctgccgc tgatgcggaa tacataattg aaaatctctg cggcgaccgg ctggtctcaa
     5341 aaaaacggta cacgcttgaa gccgggggaa tgacctggga aatcgacgaa ttttcagggc
     5401 tcaacgaggg tttgatcgta gctgaaatcg aactccaaag cgaagatcag tccttcagta
     5461 aaccggcgtg gctgggggct gaagtcacgt ttgatccgcg gtataccaac gcggtgctgg
     5521 cccgccaccc cttcacacgg tggtgatttt gcaatgaatt tttaaagaaa ccgcttaaca
     5581 taagaacatt aaagagtaac atgccgcgaa aataacttac ccattaatga ggggagaaaa
     5641 tgaaaaaagc ggttatcata ttgtttgcgg gtatggtgct gaacagcggt tacgctgagt
     5701 tgttcacgtt caacatgatc aataaaaacg gcgaatcctg ggacggattt tcagtcggag
     5761 tttataccaa caccggcggg ctgacccttg aagcttccct gtctgcttat ttaaacggaa
     5821 actacagctc agggagtcag cttaattcga cggtgtcgga attcggcatc aatgctgcgg
     5881 gcagcggtga tgatacggcc ctctttgata ccaataacgg acaggaagcc ctgtgggttt
     5941 cgttcaaccg ggcggtaacg atcaagtcaa ttacggtttc cagctttacc ggcggaaatg
     6001 ttgaaaccgg tgcttatcag gttgctgacg gaagtcttgt taattttacc gccagcggca
     6061 tctacacggt tgattcaatt ctgagccaag gatcctattt caaggtaatc gctgtaaatg
     6121 agggtggagg aaacggctgg agtcttaact ccttcacggt agaagctgtg ccggagccgg
     6181 cgacagttgc catactcagt ttcggcgggc tggctgcctg ccttttgcgc cgggtattgt
     6241 tctcgtaagt ctgcccggca ggcggcagga aacgccccgg gttccggggt gtttttattt
     6301 ttctggatat tggaaaaacc ggcgggtgcg gttacactgc tcccatgaac atttttgaaa
     6361 aaatcgtaaa ccgcgaaatc ccggcaacca ttgtctatga agacagcgac acgcttgcgt
     6421 tcatggatat tggtcctgtc atcaaagggc acacgctggt tattccgaaa acctgctacg
     6481 atccggtcac tgaaacgccc gatgaggttg tcgccaagct aatgcttgtc gcgaaacgca
     6541 ttgccgccgc acagatcaaa gggttggggg ccgacggcgt caacattatc cagaacaacg
     6601 gcaaagccgc cggacaggtg gtgccgcata ttcattttca tgtcatcccg cgttttgaag
     6661 gcgacgggca ccactggaac tgggcggcca agaaatatga cagccctgct gaaatggaaa
     6721 aacttgccgg acaaatccgc gaagggctgg ccggatgaag cacccgaacg ccgaagaact
     6781 tttacagggt attctggatc gtgactcgcg ctatacggtc gaggcctata cgtttgtccg
     6841 tgccggactc gattacaccg ttcgccgcct tgaaacgccc cgtcatgtca gcgggcagga
     6901 actgctcgac ggcattcgtg aattcgccct ggctgaattc ggaccgatga ctaagacggt
     6961 tcttaacagg tgggggatta tgagaaccga agacgtcggc gaaattgttt tcaacatggt
     7021 ggagaccgga ttgctcggaa aaaccgacaa agacagccgc gctgattttg ccaatggata
     7081 tgatttcgac gaagcgttcc gcaaaccctt ccagccgcgc agcgcgaacc ggaaagccta
     7141 actgataaag tttccggcag tttccagaat gctgtcaata tccgcctccg taacacccga
     7201 aaaccaggtg ccttgcggat ggagcagaat attcgggcct ttcgtgcaaa gaccgagaca
     7261 tccggttgtt gaaacccgta ccttgcccgt ccagccgcgt tcttttaccg cggccttaag
     7321 tttactcttc agtgcgccgc cgccctttgt tccgcagccg gggtttgcgg gatgattttc
     7381 ccggatattc gagcagatga agatgtggca aaggtaaggc gtcgactgtt tgttcacacg
     7441 gctccttccg attttaaaag cagaactttc cacgggaggc cgctgccgaa accgccgagt
     7501 ccgtttttgg cggtgacacg gtgacaggga ataaaaaccg gcagcggatt tgctccgcag
     7561 gcggtaccga cagcgcgtac cgctttcggg cgtccgatcg ctgcggcgat ctcgccatac
     7621 gtgcgcgtct gtccgcgcgg aattttctct agcgcatccc agaccgcttg ctggaacggt
     7681 gtgcttccga ggacgctact cttcgggaac attttttcaa ccatttgaaa atccactgca
     7741 ttgtccagaa aggcaaattt tctgcacggt tcggttttga gcggaggcaa attcagctca
     7801 accgctttgc cgtcaccgtc cagcgtcagc gtgatcggnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnncg gcgtcggttt tatgctcttc agcaggctga acggtctgtc
     7981 tgcgttcaac cggtctgagt tgttggtccg gctgaaccgt ttgagtcgtc ggcgacacgg
     8041 cggtttttcg ttcttcaacc ggccggacag tttgttttcg ttcaatcggc ggggtgccgg
     8101 tggtggttcg gtgttccgca gtcggctgag tcgttctgga tgttgccgcc ggagattccc
     8161 agcggttccg gttgtcgctg taggtatgca ctgcggttga ctgttgggat atcttctgcc
     8221 gcgcatcggc gtcgatccgt tcgtatttca tcggggtctt ccggctggcg acagacgcta
     8281 tgggctgaac cagctgagaa gcctggcgtt gcggttcggg cagtctggac agccgggatt
     8341 cctgttcgcg gaacgtatgc gccggacgga gatttctgtc gtcgcagcgc cggcggtatt
     8401 cattccgctc atgctcttcc cagcgcggtt cggcatgatg tctccagcgg tcatgttcgt
     8461 agatcgggtc gtaccaggtg tggtaccggc ggcattcaaa ccacgggaaa ataccgatgt
     8521 tcaggtagac gctgtcgtag taatcgccga agtaatagtg gcagtagcgc gggtaggcga
     8581 acaggctgac ctgcaacagc cccacgtcga taacgatgct gggcgaaaac gtaaatccga
     8641 cccgcccgta aactgccggc ggaaaataaa ccggcgcaaa caaaacgccg cggcgttcca
     8701 gcgcgtagtc ccagtgtcct tcggcgaaga tataaccgcg cggggtcata atgtagtgcg
     8761 acggcaccca gacccagttc ggctgcgcag tcagccagta gccggcccgc cggatatacc
     8821 ggccttcaga ccagtacatg cagggcggaa cccagatcat gtccggagac ggctgaagag
     8881 taaccggctc cagatcttcg actgtcggcg gggcgggcag atatacaatc tcccgggtgt
     8941 tggccgccgc ccagtaaccg gcaacccatt cccagccgcc ggagacctgc atccagtacc
     9001 ccggcaccca ggacatattc ggcggagctg cacgccagca ggcgctgacc cagatgtaac
     9061 ccgaccggtc cgcatcccag gaccagtatc ccggaatcca gacatactga gacccttgcg
     9121 gtttttctgc cggcggaact tcctgaatat tggccggcgg ctgctgcgga acaatcagcc
     9181 ccgcctgagt ctgcagggtg accggttcgg caaaagcctc atgcaccgga cctcggctca
     9241 aaacttcggg ctgctcctga gccggtgttg ccggaggagt cgtagagata tcctgtgccg
     9301 acaactggtg actgcccagt gatgtcgctg cgagaaatga aatgatccaa gatgcagaac
     9361 gagaacggta gttgctcata atcgacgctc cccggttaaa tgttcctgtg gttccgagtg
     9421 atggtccgat caaaaagacc tcatcaaacc gccggaaata tagtccaaac cgagaaatat
     9481 agcgaatata aacaggatgc ggtaaaacac ggctctgcta cagaccggga atggtaagcg
     9541 tctggccgac ctttatgtcg gtttcgcttt tcattttgtc gcggttggca tcgtagattt
     9601 ttttccattt ccctgtgtcg ccgtaaaccg atccggcgat ccggccaagg gtatcgccgg
     9661 ggcggacctg ataggtgcgg gctgctttta ccgggtccgc tgctttgacg gcaacaggcg
     9721 ctgcagccgg agccgtcgca ggccgggctg ccggtggctg ggtcagaagg gttttcacat
     9781 tggcgaccgc tgccggagca gctccgccgg cggtctcgag ctgcttgcgg agcatattgt
     9841 tttcgcgtgt cagccgtacc agttcttctg aaacatcgcc gccggatatg ccggcctctc
     9901 cggacagcga aattttcgcc tgacggatcc agtcctgaat cagcgcgcgt ttttcggtga
     9961 gcggacgttt ttccagatag cgttcatagt ggtaaatcgc gcgggcataa ttcttttcgt
    10021 gctggtgata aatcatcgcc agttccagat cgggtcggcc gagttccggc ttttttgcca
    10081 gcgctgtttc gaactgcccg atggctgcgt cccagttttt ctgttgaagc agttcaagcc
    10141 ccttgcggac gtgactgtct ttttcgtctg ccgtgtcgag ctgtgcgccg ctcttctggc
    10201 cgcatccggc gagcagcagt gcgcaggcga tgataaaaaa gaactttttc ataatcagaa
    10261 gaacagtttg aagaatttca gaatgccctg actccacggt acgcggtgcg agattccgcc
    10321 tttcccttcg aaggtatgca ggttggcgag gtaccactca tagttgcaaa ccagcgcctg
    10381 cttattggaa aacttcggat ggaaattcag cttctgcgcg atcttgtcgg tcgagacgaa
    10441 cgaatcttcg caggcggttt cgtagaccca cttatagagc ggcgagagct tgaagaactc
    10501 aagaatgcgc agcgcccaga tcagcggccg ggccggagtg ccgattactt tcttgccgaa
    10561 gccggcgcgg tcaagcaccg cctgatagtc ctctttcatc gtcgtaaagt ccgttgctcc
    10621 gacgttgaag atatcattca ccgccgcctc gtctttggtg agacagagcc agatcgcttc
    10681 acagaggtct tcgacggcga gcagctggta gcggttattg ccgcttccga tcatcgggaa
    10741 attcttgccg tcttttgccc agtcgtagaa cagggcgaac acgcccagcc gttccgggcc
    10801 gacgaacgac ttcgggcgga tgatcggcac gcacattcct ttggcgcggt attccagaca
    10861 gaccttttcc gcttcgatct tggcgtggcc gtacgggccg acgccgatca tcgggtcggt
    10921 ttcaaacagc gggtgatgat ccggaatccc gtaaacggcg gtcgacgaga tatgcacgaa
    10981 ccgttcaacc ttttcctttt ccgccgccgc cagcagaata cgggtacctt caatgtcggt
    11041 ggaaaaaatt tcttctttcg tatacagggg cagggccgcc gccgtgtgaa cgacccagtt
    11101 cacgcccttc atcacctcgc ggacggttcc ggcattacgg atgtcgccga ccaccgcatt
    11161 aatccggtcc cgttccggat aatcgaactc cacgagatcc agcacggtga tgtcggttac
    11221 gccctgacgg agcaggtagc ggatcatatt aatgccgagg aacccgcttc cgccggtgac
    11281 caaaaccttc ttgttcataa aacgctcctg aactagactc aatgtgcatg aaaacactga
    11341 aagacgtcgg agaacataac gccatcaagg cattgactgc aaacctgaaa gccgtcggcg
    11401 acgactgcgc cgtcctgccg ctcgacgcga actatgatct cgtcctcacc accgacccgc
    11461 tcatcagcgg cgtccacttc acgcccggca ccgatcccgg acagatcggc tggaaagccg
    11521 ccgcccgcgt actgagcgac ttcgccgcca tgggcgccga cccgcagtat attttgatca
    11581 accttgtcgc gccgccggaa caggattttc aaacgctgga aaaaatctac gccggagttt
    11641 cccggatccg ggaactgttc ggcgtggatc ttgtcggcgg cgatctcgcg caaggaccga
    11701 ctcttgaact gcacgtcttc ggcgtcggac gcgtaccgaa aggcaaagcg cttctccgtt
    11761 ccggcgcgcg ccccggcgac gtcatctacg tcaccggccc gctcggcggc tccttcgaaa
    11821 gcggcaagca cctgactttt atgccgcgca tcaaagaagc caaatggctg cggcagtccg
    11881 gtgtcgttcg ctccatgatg gatatcagcg acggactcgc caccgacctg cgccacattc
    11941 tgaaagcctc caaagtcggc gcggcg
//
LOCUS       DAOPZO010000050        11863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_6111_length_11863_cov_9.670393, whole genome shotgun sequence.
ACCESSION   DAOPZO010000050 DAOPZO010000000
VERSION     DAOPZO010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 11863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11863
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6111_length_11863_cov_9.670393"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..1430)
                     /gene="serS"
                     /locus_tag="PKI68_04715"
     CDS             complement(159..1430)
                     /gene="serS"
                     /locus_tag="PKI68_04715"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNX53027.1"
                     /translation="MLDIKFIRENPDAVKKALAARKADADIDGLLALDTARRAAITEA
                     EELKSTRNSASKAIGGLMKEGKKAEAEAAKEEVRKIGDCIAEIDERVRELDEKILNTL
                     LFIPNLPLAGVPVGASEADNPVVRSWGEKKTFGFKVKPHWDITEALKLVDFERGTKIT
                     GSGFPVYTGQGARLQRGLVQFMLDLHTIEHGYTEVEPPFVCNADAMTGTGQLPKFAED
                     MYSIPLDGLYPVPTAEVPVTNLYRDEIIDVEKPLKLTAYTPCFRREAGAAGRDTRGLL
                     RLHQFDKVEMVNFVKPEDSEKQHEILVREAEKVLQKLGLHYRVIELCTADIGFSAAKC
                     YDIELWAPGVEKWLEVSSVSNFWDYQARRARIRYRDENGKPQFIHTLNGSGVALPRLV
                     VALIENNQNADGTVTIPEALRPYMGGLEKLG"
     gene            complement(1447..2403)
                     /locus_tag="PKI68_04720"
     CDS             complement(1447..2403)
                     /locus_tag="PKI68_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNX53028.1"
                     /translation="MNFLGVDIGGTKMALSIGNERGEIRVDQRFPTDAGSAQKTLEQA
                     LKVADGLIAEAGLRVEQIDAIGISSPGPMCSRRRMILETPNLKGWNQFKVGDFFENHF
                     KRPVFMQNDANGAGLAEYMFGACKGLDLVYLTMSTGIGAGIVINGRVLAGTNDLGGEV
                     GHNTLDLSGPRCGCGKTGCWEAYCGGKNFADQIRAEITAQAVKTSILDFAGGDPARIS
                     MKEICAAVRGGDAYACCKWDVFIEKTAQAVGILLQTLNPQAVVMGTIAMHAGDLFLPQ
                     MMERLPKYAWQSCIDVCRIEASVLTNIGELSAIAIAIDGTRI"
     gene            complement(2477..3358)
                     /locus_tag="PKI68_04725"
     CDS             complement(2477..3358)
                     /locus_tag="PKI68_04725"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNX53029.1"
                     /translation="MTLLAEFMLQTKPFWEYSNPPPGTVAAALFFLLLICVGIALDAG
                     LVLHWIKRPVRLPELAASLSARALPPRLVLIFFVSMTGFYLLASWLYMFKFPSGEIGP
                     EAVIFQTLFFHLPVLILLGLLFRHAGIQWRELFGFHWKKSPALLGLSILFYLAALPLL
                     WFYSALYQILLRQFGCDFYLQDVAQILMAPAPWFTRTCLFFITIIVAPAFEEIVFRGI
                     LLPFAVRRAGFWPGMILISIFFGGLHLHLPSFFPLFLLSMMFSLAYARTRSLLVPIGM
                     HAAFNGVTVLLLLLAGG"
     gene            3438..4544
                     /gene="asd"
                     /locus_tag="PKI68_04730"
     CDS             3438..4544
                     /gene="asd"
                     /locus_tag="PKI68_04730"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF005144.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNX53030.1"
                     /translation="MKTGLVGWRGMVGSVLMERMLAENDFAGIDPVFFTTSQAGQPAP
                     DVGRGKSVLLDASDLDALGNMDVIITCQGGDYTQAVHGKLRAAGWKGYWIDAASTMRM
                     NKDAVIILDPVNRPVIDNALADGKKDFIGGNCTVSLMLMALGGLFKAGLVEWLSSMTY
                     QAASGAGAQNMRELLSQMGTLHQTAYADLQTPASAILDIDRKVTASLRGTEIPTQHFG
                     APLAGSLIPWIDKAVEDGQTKEEWKGFAETNKILGTRTAIPIDGLCVRIGAMRCHSQG
                     LTIKLKKNVPLEELIEIIRNDNEWVDVVGNTKEETLARLTPAAISGTLTVPVGRIHKM
                     KMGPEYLAAFTCGDQLLWGAAEPLRRMLRILQGS"
     gene            complement(4600..4767)
                     /locus_tag="PKI68_04735"
     CDS             complement(4600..4767)
                     /locus_tag="PKI68_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53031.1"
                     /translation="MFSKATALILIGIALAGCSTPSYDDGTAKEYSDMPWNTPARWEG
                     SMNIPGMNDYR"
     gene            complement(4827..5756)
                     /locus_tag="PKI68_04740"
     CDS             complement(4827..5756)
                     /locus_tag="PKI68_04740"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNX53032.1"
                     /translation="MDLINSVYPDKQIDMTVIQKLKSAGRPLISVEFFPPKTPAAQSI
                     FNKGAVELTGLNPDFVSVTCGAGGSAAGPTLEISKQLRGLGYGTVMPHCTCVGVSRDE
                     LARSAEETVTQGFENIMALRGDPPRGEKRFRPVADGFRHASELVAFLRERHPKLCIGV
                     AGYPEKHPESPGIEEDILCLKAKVDAGADFITTQLFLHNHVYFEFVEKCRSAGITVPI
                     VPGLLPVISLEQITRMQTFCAFHVPGKLLHSLEAAQNDPVKMERIGLYWAIEQISELV
                     ESGAPGIHLYLLNRAQTAFYPELFACLSRVRGR"
     gene            complement(5798..6079)
                     /locus_tag="PKI68_04745"
     CDS             complement(5798..6079)
                     /locus_tag="PKI68_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNX53033.1"
                     /translation="MAKAATKSQIIAKIADDAGISKVQAKSALESIIAQAYKGAAAGF
                     TVPGLGKLVKVKRKARMGRNPATGEQIKIAAKTVLKFRIAKAAKDAVLG"
     gene            6312..6770
                     /locus_tag="PKI68_04750"
     CDS             6312..6770
                     /locus_tag="PKI68_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNX53034.1"
                     /translation="MNLKKILTKDSILPELKADNKQGVIEEMVAFMAATGKLTGCESV
                     VQAVLAREEKMSTGMQNGIAIPHGKTNKVDHLVAMMAIHRAGVEFASMDGNPSHIFIM
                     TLSPENRAGPHIQFLAEISKALSRQELRESLLRAGSSDEIFDLLTAQVPA"
     gene            6943..9393
                     /locus_tag="PKI68_04755"
     CDS             6943..9393
                     /locus_tag="PKI68_04755"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNX53035.1"
                     /translation="MKLLRPRLSFTGILAALTSIVVPAARASEADINIPDLSTVSFFD
                     GKISGMSVLMIGLAVCLAGVAYGWLQYIQTRRLPVHESMHKVSKVIWETCKTYLFQQG
                     KFLTMLWILIAVCMTYYFGVLSHLSAAHVALILSASVLGILGSYGVAWFGIRINTVAN
                     SRTAFSALKGNPLATMCIPLKSGMSVGLLLVAIELFFMICILAFLPKELAGPCFIGFA
                     IGESLGASALRICGGIFTKIADIGADLMKIVFNLPEDDPRNPGVIADCTGDNAGDSVG
                     PTADGFETYGVTGVALIAFLALALAANPALCGVLIVWIFAMRILMVITSLASYLANTA
                     VSKVLYGGSKDFNLEQPLTNLVWVTSLISILVTYGASYLLLAHHPACGADLWWVLSTI
                     ISLGTIAGALIPEFTKVFTSTESRHVKEVVTSSRQGGASLNILSGLVAGNFSAFWTGL
                     VILALMFGAYHFAMSDALQNMMPEAFRFAAPIFAFGLVAFGFLGMGPVTIAVDSFGPV
                     TDNAQSVYELSQIEGRAGISGEIEREFGFKPDFENAKHQLEKGDGAGNTFKATAKPVL
                     IGTAVVGATTMVFGIIMMLKGIYPDTIEKLSLVHPEAIMGLLMGGAVIYWFTGASTQA
                     VVTGAYRAVVYIKENMKLDAATASIEASKEVVRICTQYAQRGMINIFIVIFCFSLSLA
                     LFDPFFFIGYLVGMAFFGLFQAIFMANAGGAWDNAKKIVEVELKMKNTPLHAATVVGD
                     TVGDPFKDTSSVSLNPVIKFTTLFGLLAVEIAVKMEPGLKHALGAAIFVVALIFVYRS
                     FYGMRIPVDAKDAEVKNG"
     gene            9501..11381
                     /locus_tag="PKI68_04760"
     CDS             9501..11381
                     /locus_tag="PKI68_04760"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HNX53036.1"
                     /translation="MQTVEMNRRKTAGLIVCALGVVFGDIGTSPLYALRECFHGAHGL
                     VPTAPHVLGVLSLVIWTLILIVCLKYVSFVLRADNHGEGGILALFALTRADEAGHGKR
                     RTILVMTALLGASLLYGDGMITPCITVLGAVEGLNVATPFFEPWIIPLSLGILIAIFA
                     VQHKGTGKVGGVFGPVMLVWFAVLALLGLRGILMAPEVLAALSPLYGIDFLLHSGKIG
                     FIVMGAVFLTVTGAEALYADLGHFGPRPIRLGWFFIAFPALVLNYFGQGALILKQPEA
                     LTNPFYLLAPGWALYPLVALATAASIIASQALISGAFSLTMQAIQMGYLPRMEVRHTS
                     SEQRGQVYMPTINGFLLTCCLALTLGFRSSSNLASAYGVAVTLTMIATTVLFFIVARK
                     RWGWHVLGASALCAGLLVIEILFFAANVLKIWRGGWLPLVVAAGFLAVMTTWRTGRHM
                     LGRHMKERLLPLESFLDDLSLGHAVRVPGTAVYMSGNLLGTPLALLHNFKLNRSVHER
                     VILLTILAAEEPYVDPATRVQVETLTNGFYRATARYGFMEKAAIGEIFAGCNKHGLDL
                     RPNACTFVLSRETLVSTHAKGMAMWRKRLFGLMSRNAQSATAFFGLPANRVVEMGMQI
                     EF"
     gene            11444..>11863
                     /locus_tag="PKI68_04765"
     CDS             11444..>11863
                     /locus_tag="PKI68_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53037.1"
                     /translation="MSKKLFLLDGMALVYRSYFAFMRNPMINSKGRNVSAVFGFLSTL
                     LELLEKQSPTHIAVAFDVSGPTFRHEKFPEYKAQREETPEEIRAAVPCIQEFLKAFNI
                     PILTVQGFEADDLIGTLARRAEKEGFDTYMVTPDKDFT"
BASE COUNT         2464 a         3451 c         3304 g         2644 t
ORIGIN      
        1 agcctgccaa taaaaaggcg gacaggaaaa agaagtaacc catgcggaag gcgggctgcc
       61 caggttttcc ggggcagccc atcagaacgt tcagataaaa gccccggttc caatcactgg
      121 aaaccggggc ttttttgtgc cgttttgcgg ctaactgcct accctaattt ctcgaggccg
      181 cccatgtagg ggcgcagggc ttccgggatg gtgacagtgc cgtcggcgtt ctggttgttt
      241 tcgatcagcg cgacgacgag gcgcggcagg gcgactccgg aaccgttgag cgtgtggatg
      301 aactgcggct tgccgttttc gtcgcggtag cggatgcggg cgcggcgggc ctgatagtcc
      361 cagaagttgc tgaccgatga gacttcgagc catttttcga cgccgggggc ccagagttcg
      421 atgtcgtagc acttggcggc gctgaagccg atgtcggcgg tgcagagttc gatgacgcgg
      481 tagtgcagtc cgagtttctg caaaaccttc tccgcttcgc ggacgaggat ttcgtgctgt
      541 ttttcggaat cttcgggttt gacgaagttg accatttcga ctttgtcgaa ctggtgcaga
      601 cggagcaggc cgcgagtatc gcgtccggcg gcaccggctt cgcggcggaa gcacggggtg
      661 taggcggtga gtttgagcgg cttttcaacg tcgatgattt cgtcgcggta gagattggtg
      721 accggcactt cggcggtcgg caccggataa agaccgtcga gagggatgct gtacatgtct
      781 tcggcgaatt tcggcagctg tccggtgccg gtcatggcgt cggcgttgca gacgaagggc
      841 ggctcgactt cggtgtagcc gtgttcgatg gtgtgcaggt cgagcatgaa ctggacgagt
      901 ccgcgctgca ggcgcgcacc ctgccccgtg taaaccggaa agccggagcc ggtgattttg
      961 gtgccgcgtt caaagtcgac gagcttgagc gcttcggtga tgtcccagtg cggtttgact
     1021 ttaaacccga aggttttctt ttcgccccag ctgcgcacga ccggattatc ggcttcgctc
     1081 gcgccgaccg gtacgccggc gagcggcagg ttgggaatga aaagcagggt gttcagaatt
     1141 ttttcatcga gttcgcggac ccgttcgtcg atctcggcaa tgcagtcgcc gattttacgg
     1201 acttcttctt tggcggcttc ggcctcggct tttttgcctt ctttcatgag tccgccgatg
     1261 gctttggatg cgctgttgcg ggtactcttc agttcttcgg cttcggtaat ggcggcgcga
     1321 cgggcggtgt cgagcgccag cagtccgtca atgtcagcgt cggcttttcg ggcggcaagc
     1381 gcctttttga cggcgtccgg gttttcgcgg atgaatttga tgtcgagcat gatggttatc
     1441 ctttgttcag atgcgggttc cgtcaatggc gatggcgatg gcgctgagtt cgccgatatt
     1501 ggtgagtaca ctggcttcaa tccggcagac gtcgatgcag ctctgccagg catatttggg
     1561 cagacgctcc atcatttgcg gcagaaaaag gtcgcccgca tgcatggcaa tggtgcccat
     1621 gacgacggcc tgcgggttga gcgtctgaag aagtatgccg acggcctgtg cggttttctc
     1681 gatgaaaaca tcccatttac agcaggcgta ggcatcgccg ccgcggacgg cggcgcagat
     1741 ttctttcata ctgatgcgag cggggtcgcc gccggcgaag tcgaggatgg acgttttcac
     1801 ggcttgagct gtgatttcag cacggatctg gtcggcaaag tttttgccgc cgcaataggc
     1861 ttcccagcag ccggttttgc cgcaaccgca gcgcggcccg ctgaggtcga gcgtgttgtg
     1921 gccgacttcg ccgccgaggt cattggtgcc ggccagcacg cggccgttta tgacgatgcc
     1981 cgcgccgatc ccggtgctca tggtgagata aacgaggtcg aggcctttgc aggcaccgaa
     2041 catatattca gccagcccgg cgccgttggc gtcgttctgc ataaagaccg ggcgcttgaa
     2101 atgattttca aaaaaatcgc cgactttaaa ctggttccag cctttgaggt tcggcgtttc
     2161 aaggatcatg cggcggcggg agcacatggg gccgggcgag ctgattccga tggcgtcgat
     2221 ctgttcaaca cgcaggccgg cttcggcgat cagcccgtcg gcaaccttga gcgcctgctc
     2281 cagcgttttc tgcgcgctgc ccgcatcggt ggggaaccgc tgatcaaccc gaatttcgcc
     2341 ccgctcgttt ccgatggaaa gcgccatttt ggttccgccg atatctacgc ctaaaaaatt
     2401 catgatttct cttaacccag ttcaaagacc atcgcttttt acccgtccgc cggaagcttt
     2461 caatcaaatt atcggtttat ccgcccgcca gcagaagcag aagcaccgtg acgccgttaa
     2521 aggccgcatg cataccgatc ggtaccagca gcgaccgggt gcgggcgtaa gccagactga
     2581 acatcatcga gagcagaaac agcgggaaaa acgacggcag gtgcaaatgc agtccgccga
     2641 agaaaataga aatcaggatc atgcccggcc agaaaccggc gcggcggacc gcgaacggga
     2701 gcagaatgcc ccggaacaca atctcttcaa aggccggcgc cacaataatg gtgatgaaaa
     2761 aaaggcaggt tcgggtgaac cacggcgcgg gagccatcag tatctgtgcg acatcctgca
     2821 ggtagaaatc acagccgaac tgccggagca gaatctggta gagcgcgctg tagaaccaga
     2881 gcagcggaag cgccgccaga tagaaaagga tcgaaaggcc cagcagtgcc ggcgatttct
     2941 tccagtgaaa gccgaaaagt tcgcgccatt gaatcccggc atggcggaaa agaagcccca
     3001 gaagaatcag taccggcaga tgaaagaaaa gcgtctggaa aatgacggct tccggtccga
     3061 tttctccgga cgggaatttg aacatataaa gccatgaagc cagcagatag aatccggtca
     3121 tcgatacgaa aaaaatcagt accagccggg gcgggagcgc gcgggcagag aggctggccg
     3181 ccagttccgg caggcgtaca ggccgtttga tccagtgcag aaccagcccg gcatccagcg
     3241 caattccgac gcagatcagc agcagaaaaa aaagagccgc cgccaccgtt cccggcggcg
     3301 gattgctgta ctcccaaaac ggcttggtct gcagcataaa ttcagctaaa agcgtcatag
     3361 tttgcggaca atagcggctt gcgttcattt tttaaatcaa ataccgtgac cgccttttaa
     3421 actccggggg aaatgaaatg aaaacaggtt tggtgggatg gcgcggcatg gtcggctcgg
     3481 ttctgatgga acggatgctg gcggaaaatg atttcgcggg gattgatcct gtctttttta
     3541 caacctcaca ggccggacaa cccgcgccgg atgtcggccg gggtaaatcg gtgcttctcg
     3601 acgcatcgga tcttgatgcc ctcggaaata tggacgtcat tattacctgt cagggcggtg
     3661 actatactca ggcggttcac ggcaaactgc gcgccgccgg ctggaagggc tactggatcg
     3721 acgccgcctc gacgatgcgc atgaataaag acgccgttat cattctcgat cccgtcaacc
     3781 gccctgtcat cgataacgcc ctggccgacg gcaaaaagga tttcatcggc ggcaactgca
     3841 ccgtcagcct gatgctgatg gctctcggcg gactttttaa agccggactg gttgagtggc
     3901 tttcttccat gacctatcag gcggcctccg gagccggcgc gcagaatatg cgcgaactgc
     3961 tcagtcagat gggcacactg catcagaccg cttacgccga tctgcaaacg ccggcttccg
     4021 ctattctcga tatcgaccgg aaagtgacag ccagcctgcg cggcactgaa attccgacgc
     4081 agcacttcgg cgcaccgctg gccggctcgc tgattccgtg gatcgataaa gccgtggaag
     4141 acggtcagac taaagaggaa tggaaaggtt tcgctgaaac caacaaaatt ctcggaaccc
     4201 gaaccgccat cccgatcgac ggcctttgcg tacgaatcgg agccatgcgc tgccacagcc
     4261 agggactcac gatcaagctg aagaaaaatg tgccgctgga agaactcatt gaaattatcc
     4321 gcaacgataa tgaatgggtt gatgtggtcg gcaacaccaa agaagaaacc cttgcgaggc
     4381 tgacgcctgc cgccatctcc ggaacactga cggttccggt cggacgtatc cacaaaatga
     4441 agatggggcc tgaatatctc gccgccttca cctgcggcga ccagctgctt tggggcgcgg
     4501 ctgaaccgct gcgccggatg ctgcggatcc ttcaaggaag ctaaaaccgg tgaaccggtt
     4561 ttgctgcgaa aagccttcgg gaaaccggag gctttatttt tatctgtaat cgttcatgcc
     4621 gggaatattc atgctgccct cccagcgggc cggcgtattc caaggcatat cggaatactc
     4681 ttttgccgta ccgtcgtcat aggatggagt cgaacacccg gcgagcgcga tgccgatcag
     4741 aattaaagct gtagctttgc tgaacatggg aaaaacatac gctcttttcg ccggtttttg
     4801 gaaccttttt ttccaaccat tgaaaactac cggccgcgaa cgcgggaaag gcaggcaaag
     4861 agttccggat aaaatgcggt ttgggcgcga ttcagcagat agagatgaat gccgggcgct
     4921 ccgctttcaa caagctccga aatctgttca atcgcccaat agagaccgat gcgttccatt
     4981 ttgacggggt cgttctgtgc cgcttcgaga ctgtgcagca gcttccccgg cacatggaat
     5041 gcgcagaagg tctgcatacg cgtaatctgc tcgagcgaaa tgactggaag aagtcccggg
     5101 acaatcggca cggtgatgcc cgctgaacgg catttttcaa cgaactcaaa ataaacgtga
     5161 ttatgaagaa acaactgcgt ggtgatgaaa tcagcgcctg catccacttt cgctttcaga
     5221 cagaggatat cctcttcgat ccccggtgac tccggatgtt tttccggata accggccaca
     5281 ccgatgcaaa gcttcggatg gcgctcgcgc agaaacgcaa ccagttcgga ggcatggcgg
     5341 aatccgtccg cgacaggtct gaaccgtttt tcgccgcgcg gcggatcacc gcgcagagcc
     5401 atgatattct caaatccctg tgtaacggtt tcttccgctg aacgggccag ttcgtcgcgt
     5461 gatacgccga cacaggtgca atgcggcatg acggtaccgt agccgagacc gcgaagctgt
     5521 tttgagattt cgagcgtggg gccggcggcg gatccgcctg ctccgcaggt tacggacaca
     5581 aagtccgggt tcagtcccgt cagctcaaca gcccctttat taaagatact ctgcgccgcc
     5641 ggggtcttcg gcggaaaaaa ttccaccgaa ataagcgggc gcccggcgct tttaagtttc
     5701 tggataacgg tcatatcaat ctgtttatcc ggataaacgg aatttatcaa gtccaaaaaa
     5761 agccccggcg aaccggggct gtgaatcgcg aaagcgatta accgaggacg gcatccttag
     5821 cggctttagc aatgcggaat ttgagaaccg tcttagcggc aatcttgatc tgctcgccgg
     5881 tggccgggtt gcggcccatg cgggctttgc gtttgacttt aaccagcttg cccagacccg
     5941 gaacggtgaa tccggctgcc gcgcctttat aggcctgtgc gatgatggat tcgagggcgg
     6001 atttcgcctg aactttgctg atgccggcgt cgtcggcaat cttggcgatg atctgagact
     6061 ttgtagctgc ttttgccatt gctggttctc ctgttacact ttggtttctg tttccggcct
     6121 tttcgtattt ggccccgatt tgttgggcga ttcttactgc aattgcagaa agcgcgcaag
     6181 gtgaaatgcg ataataatta agtatagcta ggggttggtg aagcttgcac cgatttttcg
     6241 agcgggtttt tcattgaccc ttttcaatag aaggccagaa tatgtttctc agtttttgag
     6301 cgggagaatg catgaatctc aaaaagatac ttaccaagga ttcgatcctg ccggagttga
     6361 aggccgataa taaacaaggt gtcatcgaag agatggtcgc ctttatggcc gccacaggaa
     6421 aactcaccgg ctgcgagtct gttgtacagg ctgtgctggc ccgcgaagaa aaaatgtcaa
     6481 ccggaatgca gaacggcatc gccattccgc acgggaaaac aaataaggtg gatcatctgg
     6541 ttgccatgat ggctattcac cgcgccggcg tcgagtttgc gtccatggac ggcaatcctt
     6601 cccatatttt catcatgacg ctttcccctg aaaaccgcgc ggggccgcac attcagtttc
     6661 tggcggaaat cagcaaggcg ctgagtcgcc aggaactgcg cgaaagcctg ctgcgcgccg
     6721 gttcgtccga tgaaattttt gatcttttga ccgctcaggt gccggcataa cttccttcat
     6781 acatatatgt cggcgccttt agcagtccac atcgggcttg acctctcggc cccgttccag
     6841 caccttctcc cccaattgta aagcgctcct tcaggacggt ttcaacgggt gcgtacccgt
     6901 atcgcctgga aagcgatccg ttatcagtat taggttaaaa acatgaagct tctgagacct
     6961 cgcctatcgt ttaccggtat attggctgca ctcacctcca ttgttgttcc cgccgctcgc
     7021 gccagcgaag ctgatattaa cattcccgat ctaagtaccg tttcgttctt tgacgggaaa
     7081 atctcgggca tgagcgtcct tatgatcggc ctcgctgttt gtttggccgg cgtggcttac
     7141 ggatggcttc agtacattca aacccgccgt cttccggttc acgagtccat gcacaaggtt
     7201 tccaaagtca tctgggaaac ctgcaaaacc taccttttcc agcagggtaa atttctcacc
     7261 atgctctgga tactcatcgc ggtatgcatg acctattact tcggagtact ttcacatctg
     7321 tcagccgccc acgtcgcact catcctgtcc gcatcggttc tcggtattct cggcagctat
     7381 ggcgttgcct ggttcggcat ccgcatcaac accgttgcca actcccgcac tgcgttctcc
     7441 gctctcaaag gcaacccgct cgccaccatg tgcatcccgc tgaagtccgg catgagcgtc
     7501 ggcctgctgc tcgttgccat tgaactgttc ttcatgatct gcattctggc gttcctcccc
     7561 aaagaactgg ccggcccgtg ctttatcggt ttcgcgatcg gcgaatccct cggcgcaagc
     7621 gccctgcgta tctgcggcgg tattttcacc aagatcgccg acatcggcgc cgacctgatg
     7681 aaaatcgttt tcaacctgcc ggaagacgat ccgcgcaatc ccggcgtaat cgctgactgc
     7741 accggtgaca acgccggcga ctctgtcggt ccgaccgccg acggttttga aacctacggc
     7801 gtgaccggcg tcgcgctgat cgcgttcctc gctctggcgc tggccgcaaa ccccgctctc
     7861 tgcggcgtac tgattgtctg gatcttcgcg atgcgcatcc tgatggtgat cacctcgctc
     7921 gccagctacc tcgccaacac cgccgtcagc aaagtgctct acggaggatc gaaagatttc
     7981 aatctggagc agccgctgac caatctcgtc tgggtcactt cactcatctc gattcttgtc
     8041 acctacggtg ccagctatct gcttctcgcc catcatcccg cctgcggcgc tgatctctgg
     8101 tgggtacttt caaccatcat ttcgctcgga accatcgccg gcgcactcat tcccgagttc
     8161 accaaagttt tcaccagcac cgaatcccgc catgtgaagg aagttgtaac ctcctccaga
     8221 cagggcggcg cttcgctcaa tattctgagc ggtctcgttg ccggcaactt ctcggcgttc
     8281 tggaccggac tcgtgattct tgcgctgatg ttcggcgcct accactttgc catgagcgat
     8341 gctctgcaaa acatgatgcc cgaagccttc cgctttgccg caccgatctt tgccttcggc
     8401 ctcgtggcct tcggcttcct cggcatgggc cctgtgacca tcgcggttga cagcttcggc
     8461 ccggtcaccg acaacgcgca gtccgtttac gaactctcgc agatcgaagg ccgggcgggc
     8521 atttccggcg aaatcgaacg cgaattcggc ttcaaacccg acttcgaaaa cgccaaacac
     8581 cagctcgaaa agggtgacgg ggccggaaat acattcaaag ccaccgccaa gccggtgctc
     8641 atcggcacgg cagtcgtcgg cgccaccacg atggtgttcg gtatcattat gatgctgaaa
     8701 ggcatctacc ccgacaccat tgagaaactc tcgctggttc atcccgaagc catcatgggc
     8761 ctcctgatgg gcggcgcggt catctactgg ttcaccggcg caagcactca ggccgtcgta
     8821 accggcgcct accgcgctgt tgtttacatc aaagaaaaca tgaagctgga tgcagccacc
     8881 gcctccattg aagcctccaa ggaagttgtc agaatctgca cgcagtatgc acagcgcggc
     8941 atgatcaaca tcttcattgt gatcttctgc ttctcgcttt cgctggcgct gtttgacccg
     9001 ttcttcttca tcggctacct cgtcggcatg gccttcttcg gcctgttcca ggcgatcttc
     9061 atggctaacg ccggcggcgc gtgggataac gccaagaaaa tcgtcgaagt tgaactgaag
     9121 atgaaaaata ctccgctgca cgccgccact gtcgttggcg acaccgtagg cgaccccttc
     9181 aaagatacct catccgtatc gctgaacccg gtgatcaagt tcaccacgct cttcggtctg
     9241 ctcgcagtcg aaattgcggt caagatggaa ccgggcctca agcatgcgct gggcgccgcc
     9301 atcttcgtcg tcgcgctcat ctttgtgtat cgcagtttct acggcatgcg catcccggtt
     9361 gatgcaaaag atgctgaagt gaagaacggc tgaaccggcc tctctgttcc ggcgaagagg
     9421 cgcgcagtta agcgcgcctc ttctgccgca ggggaacctg cagaaaaccg gaagagcccg
     9481 atgcaaacaa cggcaaaaca atgcaaacag ttgaaatgaa ccgccggaaa acagccggcc
     9541 tgatcgtgtg tgcgctgggt gtggtattcg gagacatcgg caccagcccg ttgtacgccc
     9601 tgcgcgagtg tttccacggc gcccacggtc tggttcctac cgcaccgcat gtgctgggcg
     9661 ttctttcgct tgttatctgg actctgatcc tgatcgtctg cctgaaatat gtatcgttcg
     9721 ttctgcgcgc agacaaccac ggcgaaggcg gcattctggc actgttcgcc ctcacccgcg
     9781 ccgacgaagc cggacatgga aaacgccgta ccattctggt gatgaccgct cttctgggcg
     9841 ccagtctgct ctacggtgac ggcatgatca ccccctgcat caccgtgctc ggtgcagtgg
     9901 aaggattaaa cgtcgccaca cctttctttg aaccgtggat tattccattg agtctgggca
     9961 ttctcatcgc catcttcgcg gtgcagcata aaggaaccgg caaagtcggc ggagtattcg
    10021 gtccggtcat gctggtctgg ttcgcggttc tcgccctcct tgggctgcgc ggcatcctga
    10081 tggctcccga agttctggcc gccctttcac cgctgtacgg catcgacttt ctcctgcaca
    10141 gcgggaaaat cggtttcatt gtcatgggtg ctgtgtttct gacggtgacc ggcgcagaag
    10201 ctctttatgc agacctcggc cacttcggcc cccgccccat ccgtctgggc tggtttttca
    10261 tcgcctttcc ggcactggtt ttaaattact tcgggcaggg cgcgctgatt ctcaaacagc
    10321 cggaagcgct gaccaacccg ttctatctgc tcgctccggg ctgggcactc tacccgctcg
    10381 ttgcgctcgc caccgccgcc tccatcatcg cctcacaggc ccttatctcc ggcgctttct
    10441 cattaactat gcaggcgatc caaatgggct acctgccccg catggaagtc cgccacacct
    10501 cgtcggaaca gcgcggtcag gtttacatgc cgaccatcaa cggcttcctg ctcacctgct
    10561 gtctcgcgct gacactcggc ttccgctctt caagcaatct ggcttcagcc tacggcgtcg
    10621 cggtcacact gactatgatc gccaccaccg tgctgttctt cattgtggcg cgcaagcgct
    10681 ggggctggca tgtactgggc gccagcgcgt tgtgcgcggg tctgctcgtg attgaaattc
    10741 tgttcttcgc ggccaacgta ttgaaaatct ggcgcggcgg ctggctcccg ctggtggtcg
    10801 ccgcaggatt cctggcagtg atgacaacct ggcggaccgg ccgtcatatg ctcggccgtc
    10861 atatgaaaga acgcctgctg ccgctggaaa gcttcctcga cgacctgagc ctcggccatg
    10921 ccgtgcgggt gcccggcaca gccgtctata tgtccggaaa tcttctcggc acaccgctgg
    10981 cgctgctcca caactttaaa ctcaaccgct ccgtgcacga acgcgttata ctgctcacga
    11041 tccttgccgc cgaggagccg tatgtggatc cggccacgcg ggttcaggtg gaaactctga
    11101 ccaacggctt ctatcgcgca accgcccgct acggcttcat ggaaaaagcc gccatcggag
    11161 aaatctttgc cggttgcaat aaacacggcc ttgatctgcg tccgaatgcc tgcaccttcg
    11221 tcctcagccg cgaaacactg gtatcaacgc atgccaaagg aatggcgatg tggagaaaac
    11281 gcctcttcgg cctgatgtcc cgcaacgccc agtcggccac cgccttcttc ggcctgcccg
    11341 ccaaccgcgt cgtcgaaatg ggcatgcaga tcgagtttta atctgctttt caagagtccg
    11401 gtaaaatcct tccaaccgcc ggatttttga ttacactccc cgcatgagca aaaagttatt
    11461 ccttctcgac ggcatggcgc tggtctaccg ctcctacttc gccttcatgc gcaacccgat
    11521 gatcaactcg aagggccgca acgtctccgc cgtcttcggg ttcctcagca cgctgcttga
    11581 actgctcgag aagcagagcc cgacgcacat cgccgtcgcc ttcgacgttt ccggcccgac
    11641 cttccgccac gaaaaattcc ccgaatacaa agcgcagcgc gaagaaacgc ccgaagagat
    11701 ccgcgccgcc gtgccctgca ttcaggaatt cctgaaagcc ttcaacattc cgatcctcac
    11761 cgtgcagggc tttgaagccg acgacctgat cggcacgctc gcccgccgtg cggagaaaga
    11821 aggcttcgac acctacatgg tcacgccgga caaagatttc acc
//
LOCUS       DAOPZO010000051        11845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_6120_length_11845_cov_9.687447, whole genome shotgun sequence.
ACCESSION   DAOPZO010000051 DAOPZO010000000
VERSION     DAOPZO010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 11845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11845
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6120_length_11845_cov_9.687447"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /gene="gmd"
                     /locus_tag="PKI68_04770"
     CDS             complement(<1..989)
                     /gene="gmd"
                     /locus_tag="PKI68_04770"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNX53038.1"
                     /translation="MKKALITGITGQDGSYLAELLLDKGYEVHGIIRRASSFNTDRID
                     HLYKDRHETGVKMFLHYGDLTDSSNLNRLIEKIHPSEIYNLGAQSHVQVSFEVPEYTA
                     EVDAIGTLRLLDSIRETGVNCRFYQASTSELYGMVQEVPQTETTPFYPRSPYAVAKQY
                     GFWIVKNYREAYGLHASNGILFNHESPRRGETFVTRKITMAVARISRGMQKCLYMGNI
                     NALRDWGYAPDYVRMMWMMLQQDTPDDYVVATNEMHTVREFIEKSFGHVGIEIVWDGE
                     GVKEVGKNKSTGDIVVRMDERYYRPAEVEQLLGNPAKAKKQLGWEPNVKFEELV"
     gene            complement(1008..1967)
                     /locus_tag="PKI68_04775"
     CDS             complement(1008..1967)
                     /locus_tag="PKI68_04775"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNX53039.1"
                     /translation="MKIFVTGTAGFVGMHLAESLLADGHEVVGLDNFNEYYDVSLKQA
                     RHARLEGVKGYTGVRGDLADYELLTALFQENRFDRVCHLAAQAGVRYSITNPFVYQKA
                     NLEGHLNILEACRRAKVQRLVYASSSSVYGGNKKVPFSESDPVDNPVSLYAATKKADE
                     LMSHTYTHLYGLQTVGLRFFTVYGPWGRPDMAYWSFTEAMLAGKPVPVFNHGKMERDF
                     TYITDIVAGVKAALFADGLDPYEIFNLGNSKPENLMDFIGTLADALGVEPKMEMLPMQ
                     AGDVPVTFADVSKARKKLGYNPSTQISAGLPEFVKWYKEYFNY"
     gene            complement(2076..2597)
                     /locus_tag="PKI68_04780"
     CDS             complement(2076..2597)
                     /locus_tag="PKI68_04780"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HNX53040.1"
                     /translation="MEQTPASSENFWLCVRAQPKREHIAAGRLKLLAGVEVFCPRIRF
                     QRNTKRGKVWFEEALFPGYLFARFDVRAMLRTVSGTYGVQGLVRFAGECAQVPDFVVD
                     SLRREASGVIVVKEPALKPGDTAIMAEGAMRGLRAVVTQVLPGGERVKILMELMGTSV
                     AAEVSIMALEPAA"
     gene            complement(2602..3084)
                     /locus_tag="PKI68_04785"
     CDS             complement(2602..3084)
                     /locus_tag="PKI68_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNX53041.1"
                     /translation="MDPALSELEKEVVDIFVRIAGVLSLPRSVGELYGVLFISPKPMC
                     IDDLMRKLKISKGSTSQGLKILRSFGAVNPVYMAGDRRDFFTAEAELRKIVSGFVKEQ
                     VYPHLENGKERMARIEKLLAEETPEQKEFIAERIERLQGWQKRAGALLPFALNFVKPD
                     "
     gene            3378..4646
                     /gene="nusA"
                     /locus_tag="PKI68_04790"
     CDS             3378..4646
                     /gene="nusA"
                     /locus_tag="PKI68_04790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNX53042.1"
                     /translation="MNSELSAVVEYMERERGVDRETIIGAIESALQNAANKSIADAED
                     LRVEINRQTLDIKAFARKKVVISVNDRYTEVSLAEARRTNPAAQLDEMIEIESTPRNF
                     GRIAAQTAKQTILQRIRQAEKSRIFDDYKNRTGEIITGTVRRFERSTVVVELDHGAEG
                     LMPSKERVPTEEYEVGERIRAYIVSIKERESGPEILLSRSHPDFVRRLFELEVSEIAD
                     GTMEIRGIAREAGCRSKVAVISHDERVDPVGACVGIRGMRVKNIVRELNGEKIDIVRW
                     SDDIKTLVVNALAPAKLKTVEVDEPTHTVTVTVDADQLSLAIGKRGQNARLTSKLIGW
                     RVDIQKDEEAMNFEERVAAAVHSLAAVEGIGEEWADKLVYAGFLTIEGIMAADLSDLV
                     EIEDVTPEQAQALWSAAEKAYTAKHGEIKE"
     gene            4643..6727
                     /gene="infB"
                     /locus_tag="PKI68_04795"
     CDS             4643..6727
                     /gene="infB"
                     /locus_tag="PKI68_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNX53043.1"
                     /translation="MRVHELATDLDVTVDELLEILSDVEIKAEDGQSLLADKEVAVVC
                     DELGFASIEAARAAKTAKAVKSAPAPAPAAVETPKKEPAPAPAAAATEEPAEESSSVL
                     AFAKPQIAVKELAEKMAIKPNQLIAELMRMNIFASLNQDIDIKIAKQIGEKHGFTVEL
                     KKKDKPQPKPAEAAPRKPVAPVEKDRPEDLEIRPPIVTFMGHVDHGKTSLIDRIRNTK
                     VVAGESGGITQHIGAYTVDMDARKITILDTPGHAAFTAMRARGANVTDIAVLVVAADD
                     GVMPQTIEAIKHAKAAGVCIIVALNKMDLPGTHPDRIKQQLNEHGVMVEEWGGTVGCI
                     PVSAATGAGIDALLERITLDAEMLELRANAHKRAVGFVIEAQMEPGMGPTATLLVRDG
                     TLKVGDNVICGAQYGRIKALINDQGKQVRTAGPSCAVKCLGLTGVPGAGDEFTVCANL
                     AEAKQLSETLQADIKSADLNQTSRGKVSLDDLFSQVGAGEVKELNVIVKCDVQGSVEA
                     ITHSLSGIKSDKVKLRILSSDVGNITTNDIMLASASDAIILGFHVAKENGVTAAAKKE
                     GVEIRLYSIIYELLEEVEAAMCGLLDPELRETYLGKAEIKAIFDMGKRNKVAGCMVIE
                     GKITSRASIRIKRGNEVLYKGTIGSLKRFQSDAAEVHNGQECGIRPDNFANFQEGDII
                     EAYTVEKIAQKL"
     gene            6788..7171
                     /gene="rbfA"
                     /locus_tag="PKI68_04800"
     CDS             6788..7171
                     /gene="rbfA"
                     /locus_tag="PKI68_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNX53044.1"
                     /translation="MSSARIIRVNELLKREIAVDIPRLFANSEFDTGAITVTGVETAP
                     DLHDASVYVSIFGHEDSRAKMIHFLNAHRKEIQHRMSRNVILKYTPRLHFKLDSSIEN
                     GDRVLGILAKLDIPEDSTSAAEEEL"
     gene            7203..7919
                     /gene="truB"
                     /locus_tag="PKI68_04805"
     CDS             7203..7919
                     /gene="truB"
                     /locus_tag="PKI68_04805"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNX53045.1"
                     /translation="MKKRNFLPDPDGILLIDKPCEWTSHDIVAKVRNHFQLNKTGHGG
                     TLDPNATGLVVLLIGRGTQLSARIMGGDKTYEGEILLGVETNSQDTDGEITAEKDPSG
                     VTEEQLRAEMKKAVGDQMQMPPMVSAIKQNGVPLYKLARKGQDVEREPRFIHIYKFAL
                     KEFNPPRVTFEVKSTKGTYVRTLAHDLGQRLGCGACLSQLRRTQSGDFSLSNAWKLED
                     VLKWDRAELEKNMISVSQLA"
     gene            7919..8923
                     /gene="ribF"
                     /locus_tag="PKI68_04810"
     CDS             7919..8923
                     /gene="ribF"
                     /locus_tag="PKI68_04810"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HNX53046.1"
                     /translation="MLHVKNLEAIPQTDKPVVLAMGCFDGVHIGHRKVISTAVEQAKA
                     RRGEAWVFTFNPHPAKVLSPETAPPLISAESCRLRQFEALGVHGVIAIPFDQSFAHTE
                     PEQFLAALWKKLPTLSGIVCGTDWSFGYKARGKFQSLEKLCGEHGITATAVPPVLCNQ
                     ERISSTHIRQAVHNGDIPLAEKMLGRPFCIFGTVVKGRGIGRGLGFPTANIEPENELI
                     PPPGVYAAYTKVESQKSKVKSQESNTRPSTFDLEPLTLPIPSALFIGERKTFQDSAPV
                     IEAYLLDFDGDLYGRTIEVCLVQKVRDVAAFPSKEALIAQIEKDVICINSLLKEPGSC
                     "
     gene            9025..9396
                     /locus_tag="PKI68_04815"
     CDS             9025..9396
                     /locus_tag="PKI68_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HNX53047.1"
                     /translation="MIRSRKVLVAGIFLLAVLSSQVSFAAKSCMGDPESCTKLASNKL
                     WRGVVNTFTGVGEIIRQPIVCTKTDGAVGIPVGLVNGVFMSFVRTGVGIVDVVTFPVP
                     FNDDIGYNSLLNPAYVWQKAK"
     gene            complement(9457..10332)
                     /locus_tag="PKI68_04820"
     CDS             complement(9457..10332)
                     /locus_tag="PKI68_04820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNX53048.1"
                     /translation="MKTEKAKGGAILTLMIAQSAAETISDWVAENLEKTVVELKKPGG
                     TECALEIYFDNTIEAEIAARALEHFPLRQGSGQAVKDRSIREYAAEEWAESWKKHFKP
                     MDIGRRLRVVPPWLAGQSDRIELVINPGLSFGTGNHFTTRFCLKQLDRFVPQSGAKTM
                     LDVGTGSGVLAIAAVRLGVEKALGVDFDPICIECSMENAQANGVAEQTAFRQHDITEG
                     WPDEKYDIVCANIYASLLIDNAATLLRVTGKYLLLTGIREVELDGVADTFVRLGGREI
                     VRDGDGEWSGLVFAV"
     gene            10364..11485
                     /locus_tag="PKI68_04825"
     CDS             10364..11485
                     /locus_tag="PKI68_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HNX53049.1"
                     /translation="MKTVVAETVLLGRNAFETLGDVVVIPDRQIGPGNLKDADALVIR
                     SKTKVTPELLAGSAVRFVGTATAGFEHMDFKYLEKSGIGWCAAPGCNADSVADYMTAA
                     LLTLHTKYGVELEDKTIGIIGVGQVGSRVAKRAEALGLRVLLNDPPRAAREGDTGFES
                     LEKLLAEADIVTLHVPLIKEQPWPTLKMADDSFFKKMKRGAVFINASRGKVLDSDALL
                     HAKENGIVSHAALDVWDPEPSIRADVLAAATIATPHIAGHSFEGKLNGTVQVYREACR
                     FFKTAPVWDPAPLLPEPAVPELKIDSRGKSDLEVLTEAVKAVYDIGTDCLTEKDIERF
                     DKLRAHYGVRREFKNTTVFLSETRPDLLKKIGKAGFTLA"
BASE COUNT         2876 a         3495 c         2986 g         2488 t
ORIGIN      
        1 accagttctt cgaatttaac gttcggctcc cagccaagct gctttttggc tttcgccgga
       61 ttaccgagca gctgctcgac ttccgccgga cggtaatagc gttcgtccat ccggacaaca
      121 atatcgccgg tcgacttgtt tttcccgacc tctttgaccc cctcgccatc ccagacgatt
      181 tcaatcccca catggccgaa tgatttttcg atgaactcgc gaacggtgtg catttcgttg
      241 gtcgcgacga cataatcgtc cggcgtgtcc tgctgaagca tcatccacat catccggacg
      301 tagtccggcg cataacccca gtcgcgcaac gcattgatgt tccccatgta gagacatttc
      361 tgcatcccgc gggaaatgcg cgcaaccgcc atggtaattt tacgcgtgac gaaggtttcg
      421 ccgcgccgcg gagattcgtg gttgaagaga attccgttgc tggcatgcag gccgtacgct
      481 tcgcggtagt ttttgacaat ccagaagccg tactgcttgg caaccgcata aggagaacgc
      541 gggtagaacg gagtcgtttc cgtctgagga acttcctgca ccattccgta gagctcggag
      601 gtggacgcct gatagaagcg gcagttcaca ccggtttcgc ggatggagtc cagcagacgc
      661 agcgttccga tagcatcgac ttcagcggta tattccggaa cttcaaatga aacctggacg
      721 tgtgactgtg caccgaggtt atagatttcg gaaggatgga ttttttcaat caaccggttc
      781 aggttgctgg agtccgtcag atcgccgtaa tgcagaaaca tctttacgcc ggtttcgtgg
      841 cggtctttat aaagatggtc aattcggtcc gtattgaacg aactggcacg gcgaataatt
      901 ccatgtactt cgtatccttt atcgagcagc aattcggcga ggtacgaacc atcctgtccg
      961 gtaattccgg tgatcagtgc ttttttcatg agcaaccttc ggttgggtta ataattaaaa
     1021 tattccttat accacttcac aaactcaggc agacccgctg agatctgagt cgacgggtta
     1081 tagcccagtt ttttccgggc tttggaaaca tctgcaaacg tcaccggcac atccccggcc
     1141 tgcattggaa gcatttccat cttcggctca acgcctaatg catctgccaa agtccctatg
     1201 aaatccatca gattctcagg cttactgttg cccagattga aaatctcgta cggatccagt
     1261 ccgtcggcaa aaagcgcggc cttcaccccg gccacaatgt ccgtgatata ggtgaaatcg
     1321 cgctccattt tcccatgatt aaataccgga accggtttcc cggccagcat cgcctccgtg
     1381 aacgaccagt acgccatatc cggacggccc cacgggccgt acaccgtgaa aaaccgcagg
     1441 ccgaccgtct gcaagccgta taaatgcgtg taggtgtgac tcatcagctc atccgctttt
     1501 ttcgtcgccg catacaggct gaccggatta tccaccggat ccgactcgct gaacggaact
     1561 tttttattcc cgccgtatac gctggagctg gaagcgtaga ccagtctctg tactttggcg
     1621 cggcggcagg cctccagaat attcaggtgt ccttcaaggt tggctttctg atagacaaac
     1681 ggattcgtaa ttgaatagcg gacaccggcc tgcgccgcca gatggcatac ccggtcaaac
     1741 cggttttcct gaaacaacgc ggtcaataac tcataatctg cgagatcgcc tctgactccc
     1801 gtatagccct tgacgccttc cagccgggcg tggcgagcct gcttcaagga aacgtcgtaa
     1861 tactcattga aattatcaag gcctacgact tcatgaccgt cagccagcag ggattcagcc
     1921 aaatgcatac cgacaaagcc cgcggtacct gttacaaaaa ttttcattgc gtcctttaaa
     1981 aaagctgccg cccgcggtcg ctcgggatat tccgcccgaa accttccttg ctctcttctg
     2041 ctgtatattc cgcatccaat tgcgaaactt ctatctcacg ctgccggctc cagtgccatg
     2101 atcgacactt cggccgccac agaggtcccc atcaattcca tcagaatctt aacgcgctct
     2161 ccgcccggca gaacctgggt cacaacggcg cgcagtcctc gcatcgcccc ttcggccatt
     2221 atcgctgtat cgcccggctt cagcgccggc tctttaacca caatcactcc ggaagcctca
     2281 cgccgcagcg agtccaccac aaaatcggga acctgcgcgc attctccggc aaaacgaacc
     2341 agtccctgaa caccatacgt gccggaaacc gtacgaagca ttgcccgaac atcaaaacgg
     2401 gcgaaaaggt agcccgggaa aagggcttct tcaaaccata ctttcccccg ctttgtgttg
     2461 cgctggaatc ggatacgcgg gcagaacact tcaacgcctg ccagcagctt caaccgtccg
     2521 gcagcaatat gctcccgttt cggctgagct ctgacacaga gccagaaatt ttctgaagat
     2581 gcgggggttt gttccatcac attaatcggg ttttacgaaa ttaagcgcaa aaggcagaag
     2641 cgcaccggcg cgcttctgcc atccttgcag ccgttcaatt cgttcggcaa taaattcctt
     2701 ctgctccgga gtttcttctg ccagaagctt ttcgatccgc gccatccgct ccttgccgtt
     2761 ttccagatgc ggataaacct gctcctttac aaatccgctg acgatcttac gcagttctgc
     2821 ctcagccgta aagaaatccc tccggtcgcc ggccatataa accggattaa ccgccccgaa
     2881 cgaccgcaaa atcttaagtc cctggctggt cgaaccttta ctgatcttca gcttgcgcat
     2941 taaatcatca atgcacatcg gtttgggaga gataaacaga acgccataaa gctcgccgac
     3001 tgaacgcgga aggctcagca ccccggcaat acgcacgaag atgtcgacaa cctctttttc
     3061 caactctgaa agtgctggat ccatacgctc gccctcgata attcagattg ttcaaaattt
     3121 aatgaacgta gaggcctttt tcaaggttaa ttctatttaa atcaatgcct tagaactgat
     3181 cttttcaccg ccggcatcat aaaccatcgg cgtcagcatc cggagcccct cgttcaaaaa
     3241 accccttgac cctcctctga cggaagcgta gattgccgac cttgcagttg gtcgttggta
     3301 tagagagtgg gcccccggtc cgctcttttt tgctccaagg ccttgttgtt ttagtttttt
     3361 gaaatcggga ggggatgatg aacagcgaac tgagtgccgt tgtggaatat atggaacgcg
     3421 agcgcggagt cgatcgcgaa accattatag gggcgattga atctgctttg cagaatgccg
     3481 ccaataaaag catagccgac gctgaagatt tgcgtgttga aatcaacaga caaactcttg
     3541 atatcaaagc ttttgcacgc aaaaaagtag tcatttctgt caatgaccgt tataccgagg
     3601 tcagtctggc tgaagcccgc cgcaccaacc cggcggctca actcgatgaa atgatcgaga
     3661 ttgagtccac tccgcgcaat ttcggccgca tcgccgccca gaccgccaag caaaccattc
     3721 tgcaacgcat ccgccaggca gaaaaaagcc gcatttttga cgactacaaa aaccgcaccg
     3781 gcgaaatcat caccggcaca gtacgccggt ttgagcgcag caccgtcgtt gtcgagctgg
     3841 atcatggcgc cgaaggcctg atgccttcca aagaacgcgt cccgaccgaa gaatatgaag
     3901 tcggcgaacg catccgtgct tacatcgtct cgatcaagga acgggaatcc ggcccggaaa
     3961 tcctgctttc ccgcagccat ccggactttg tccgccgcct tttcgaactg gaagtctcgg
     4021 aaattgccga cggcaccatg gaaatccgcg gcatcgcccg cgaagccggc tgccgcagca
     4081 aagttgcagt catttcacac gacgaacggg ttgatccggt cggcgcctgc gtcggcatcc
     4141 gcggtatgcg cgtcaaaaat atcgttcgcg aactcaacgg agagaaaatc gacatcgtcc
     4201 gctggagcga cgacatcaaa acgctcgtcg tcaatgcgct ggccccggcc aagctcaaaa
     4261 ccgtcgaagt ggatgaaccg acacataccg tcaccgtcac ggttgatgcc gaccagctct
     4321 cgctggccat cggcaaacgc ggccagaacg cccgcctgac atccaaactg atcggctggc
     4381 gcgtcgacat ccagaaggat gaagaggcga tgaattttga agaacgcgtt gcggcagctg
     4441 tccacagtct ggctgcggtt gagggcatcg gtgaagaatg ggcggataaa ctggtttacg
     4501 ccgggttcct caccatcgaa ggcattatgg ccgccgatct gtccgacctc gtagagatcg
     4561 aggatgttac ccccgaacag gcgcaggctc tttggagcgc tgcggaaaaa gcctacacgg
     4621 caaaacatgg tgaaattaaa gaatgagagt ccacgaacta gccactgatt tagacgtaac
     4681 cgtcgacgag ctgcttgaaa tcctcagcga tgtggagatc aaagccgaag acggtcagag
     4741 tctccttgcc gacaaggaag tcgccgtcgt ctgcgacgaa ctcggctttg cctcgatcga
     4801 agccgcccgt gcggccaaga cagccaaagc ggtcaaatcc gctccggctc ccgcaccggc
     4861 agcagtagaa accccgaaaa aagaaccggc tcccgcaccg gcggcggccg cgacggaaga
     4921 acctgctgaa gaatcgtcca gtgttctcgc tttcgccaaa ccgcagattg ccgtcaaaga
     4981 gctggctgaa aaaatggcga tcaaacccaa ccagctgatc gccgagctga tgcgaatgaa
     5041 catcttcgcg tcgctcaatc aggatatcga catcaagatc gccaaacaga tcggcgaaaa
     5101 acacggcttt acggtcgaac tgaagaaaaa agacaaaccg cagcccaagc cggccgaagc
     5161 cgcaccgcgc aaaccggttg ctccggtgga aaaagaccgg ccggaagatc tcgaaatccg
     5221 cccgccgatt gtcaccttca tgggacacgt cgaccacggc aaaacctcgc tgatcgaccg
     5281 tatccgcaac acgaaagtgg tggcgggcga atccggcggt atcacgcagc atatcggcgc
     5341 ctacacggtc gatatggatg cgcgcaaaat tacgattctc gacacgcccg gccacgccgc
     5401 tttcaccgcc atgcgcgccc gcggcgccaa cgtcacggat attgcagttc ttgttgttgc
     5461 ggccgatgac ggcgtaatgc cgcagacgat cgaagccatc aagcatgcca aggccgccgg
     5521 cgtctgtatt atcgtcgcct tgaacaaaat ggatctgccc ggcacccatc ccgaccgtat
     5581 caagcagcag ctcaatgagc atggcgtgat ggttgaagaa tggggcggaa cggtcggctg
     5641 tattcctgtc agcgccgcca ccggcgcggg catcgacgcc ctgctcgagc gcatcacgct
     5701 cgacgccgaa atgctggaac tccgcgccaa tgcacataaa cgggccgtcg gcttcgtcat
     5761 tgaagcgcag atggaaccgg gcatgggccc gaccgcgaca ctgcttgtac gcgacggaac
     5821 tctcaaagtc ggcgacaatg tgatttgcgg cgcccagtat ggccgcatca aagcgctgat
     5881 caacgatcag ggtaaacagg tgcgcaccgc cggaccgtcc tgcgccgtga aatgcctcgg
     5941 ccttaccggc gttccgggtg ccggcgacga gttcaccgta tgcgccaacc tggccgaagc
     6001 caaacaactc tcggaaacgc tccaggccga catcaaatcc gccgatctga accagacctc
     6061 gcgcggtaaa gtatcgctcg acgatctttt ttcacaggtc ggcgccggcg aagtcaaaga
     6121 gctgaatgtc attgttaaat gcgatgttca aggctcagtc gaagccatca cccactcgct
     6181 gtccggaatc aagagcgaca aggtgaagtt gaggattctc tccagcgatg tcggcaatat
     6241 caccacaaac gacatcatgc tggccagcgc ctccgatgcg atcattctcg gcttccatgt
     6301 cgccaaagaa aacggcgtaa ccgcagcggc caagaaagaa ggcgttgaaa tccgtcttta
     6361 cagcatcatt tatgagctgc tcgaagaagt tgaagccgcg atgtgcggac tgctcgatcc
     6421 cgaactgcgc gaaacctatc tcggaaaagc tgaaatcaaa gcaatctttg atatgggcaa
     6481 gcgcaacaaa gtggccggct gcatggttat cgaaggcaag attacttcgc gcgccagcat
     6541 ccgcatcaaa cgcggaaacg aagtgctcta caaaggcacc atcggttcgc tcaagaggtt
     6601 ccagagcgat gccgccgaag tgcacaacgg tcaggaatgc ggtatccgtc ccgacaactt
     6661 cgccaacttc caggaaggcg atatcatcga ggcctacacc gtcgaaaaaa tcgcccaaaa
     6721 gctgtagagg ggaaaagatg aaagatgaaa acggattcat tccgttttca tagttcataa
     6781 ttcagtcatg tcgagcgcac gtatcatccg ggttaacgaa ctgctcaaac gcgaaatcgc
     6841 ggttgatatc ccccggcttt ttgccaacag cgaattcgat accggcgcca tcaccgtcac
     6901 cggcgttgaa accgcgccgg atctgcacga cgcctccgtt tacgtctcca tcttcggaca
     6961 cgaagacagc cgcgccaaaa tgattcattt tctcaatgcg caccgcaaag agattcagca
     7021 ccgcatgagc cggaacgtta tccttaaata cacgccgcgc ctgcacttca aactcgacag
     7081 ctccattgaa aacggcgacc gcgtactcgg cattctggcg aagctcgaca tccctgaaga
     7141 cagcacgtcc gcggcagaag aagaactgtg aaccgcaacc catcggcaat cggaaatcat
     7201 aaatgaaaaa acgcaatttc ctgcctgatc cggacggcat tcttctcatc gacaaaccct
     7261 gcgaatggac ctcgcacgac atcgtcgcca aagtccgcaa ccattttcag ctcaacaaaa
     7321 ccggacatgg cggaacactt gatccgaacg ccaccggtct ggtcgtcctg ctcatcggac
     7381 gcggcacaca gctctcggcc cggattatgg gcggcgacaa aacctacgaa ggcgaaatcc
     7441 tcctcggcgt cgaaaccaac tcgcaggaca ccgacggcga aatcaccgca gaaaaagatc
     7501 cttccggcgt cactgaagaa cagctgcgcg ctgaaatgaa aaaagcggtc ggcgaccaga
     7561 tgcagatgcc gcccatggtt tccgccatca aacagaacgg cgtcccgctg tacaaactcg
     7621 cccgcaaggg acaggacgtt gagcgcgaac cgcgcttcat tcacatctac aaattcgctc
     7681 tgaaggaatt caaccctccg cgcgtcacct ttgaagtcaa atccaccaaa ggcacttacg
     7741 tccgcaccct cgcccacgac ctcgggcagc gcctcggctg cggtgcatgc ctgtcgcagc
     7801 tgcgccgcac gcagtccggc gacttcagcc tttccaacgc ctggaaactt gaagacgtcc
     7861 tcaagtggga tcgggcagaa cttgaaaaaa acatgatttc tgttagtcaa ttagcctgat
     7921 gctgcacgtt aaaaacctcg aagcgattcc gcaaaccgac aaacctgtcg ttctggcgat
     7981 gggatgcttc gacggtgtac atatcggcca ccggaaagtc atctccaccg ccgtcgaaca
     8041 ggcaaaagcc cgccgcggcg aagcgtgggt tttcaccttt aacccgcatc cggccaaagt
     8101 actcagtcct gaaaccgcgc cgccgctgat cagtgcggaa agctgccgcc tgcggcaatt
     8161 cgaagcgctc ggcgtacacg gcgtcatcgc tattccgttc gatcaatcct ttgcgcacac
     8221 ggagccggaa cagtttcttg ccgccttgtg gaaaaaactg ccgaccctca gcgggatcgt
     8281 ctgcggcacc gactggtcgt tcggctacaa agcacgcggc aaattccagt ctctggaaaa
     8341 actctgcgga gaacatggca ttacggcgac ggcggtgccg ccggttctct gcaatcagga
     8401 gcggatttcc agcacccata tccggcaggc ggtgcataac ggcgatattc cgctggcgga
     8461 aaaaatgctg ggccgcccct tctgtatttt cggcaccgtc gtcaaaggcc gcggcatcgg
     8521 ccgcggactg ggctttccga ccgccaacat cgaaccggaa aacgaactga ttccgccgcc
     8581 cggcgtttac gcagcatata caaaagtcga aagccaaaag tcgaaagtca aaagtcagga
     8641 atctaatacc cgaccttcga ccttcgacct tgaacctttg actctcccga ttcccagcgc
     8701 cctgttcatc ggcgaacgga aaacatttca ggactccgcc ccggtgattg aagcttacct
     8761 ccttgatttt gacggcgatc tttacggccg gacgatcgaa gtctgtttag ttcagaaagt
     8821 ccgcgatgtt gccgctttcc cttccaaaga agctctcatc gcgcagattg aaaaagacgt
     8881 catttgcata aatagtttgt taaaagaacc cggcagttgt tgaactttca tcttgatcct
     8941 cttcgtccgg atttgttcaa agagccatcg gttcaagagg gtacacgtta tcacaggttt
     9001 gaaattcaac agaaggagtt aggcatgatt cgttcacgaa aagtactggt cgcaggcata
     9061 tttcttttgg cggttctttc ctctcaggtt tcctttgcgg cgaaatcatg catgggagat
     9121 cctgaatcct gcacgaaact cgcaagcaat aagctgtggc gcggagttgt taacactttt
     9181 acaggtgtcg gagaaattat ccggcaaccc atcgtttgca caaagactga cggcgctgtt
     9241 ggaattcccg ttggactcgt caacggagta tttatgtcgt tcgtaagaac aggcgtcggc
     9301 attgtagacg tggtcacatt cccagtgcct ttcaatgacg atatcgggta caacagcctt
     9361 cttaaccctg catatgtctg gcagaaagcc aaatagcggg cgctgacggc aaaacacaat
     9421 cgccaaaaac cctgctcgga tgagcagggt tttttattag accgcaaaca ccagccccga
     9481 ccactcgcca tcgccgtccc ggacgatttc ccgtccgccc agccgcacaa atgtatcggc
     9541 cacgccgtcg agttccactt cgcggatacc ggtcagcagc aggtattttc cggtcacgcg
     9601 caaaagcgtt gccgcattat caatcagcag cgacgcgtaa atattggcgc agacaatgtc
     9661 atacttctca tccggccagc cttcggtgat atcgtgctga cgaaaagcgg tttgttcagc
     9721 cacaccgttc gcctgcgcgt tctccatcga gcattcgata cagatcgggt caaaatccac
     9781 acccaaagcc ttttcaacgc ccagtctgac ggcggcaatc gccagaacac cggagccggt
     9841 gccgacatcg agcattgttt tcgcaccgct ctgcggaaca aaccggtcga gctgtttgag
     9901 acagaaccgg gtcgtaaaat ggttgccggt tccgaaactc aaccccggat tgatcaccag
     9961 ctcaatgcgg tcactctgcc cggccagcca gggcggaact acgcgcaacc gccggccgat
    10021 atccatcggc ttgaaatgct ttttccagct ttccgcccac tcttcagcgg catactcacg
    10081 gatcgaccgg tctttgaccg cctgccctga gccctgccga aggggaaaat gctccagcgc
    10141 ccgcgcggcg atttccgctt cgatggtatt gtcaaaatag atttcgagcg cacattcagt
    10201 accgcccggc tttttcagct ctacgactgt tttttccaga ttctccgcca cccagtcact
    10261 gatcgtttcc gcagcactct gcgcaatcat cagcgtcaga attgctccgc cttttgcttt
    10321 ttccgttttc atgtgcaggg cttataccgt aaattccctg ccaatgaaaa ctgttgtagc
    10381 agaaactgta ctgttgggcc gcaacgcgtt tgaaacgctc ggcgacgtgg tggtcatccc
    10441 tgaccggcag atcgggccgg ggaacctcaa ggatgccgat gcactcgtca tccgatcaaa
    10501 aaccaaggtg acaccggaac tgctcgcagg ttccgctgtc cgctttgtcg gcaccgccac
    10561 cgccggattt gaacatatgg atttcaaata tctggaaaaa agcggaatcg gctggtgcgc
    10621 agcgccggga tgcaacgccg acagcgtcgc cgactacatg acggccgctc tgctgactct
    10681 tcacaccaaa tacggcgttg agctcgaaga taaaaccatc ggcatcatcg gggtcggaca
    10741 ggttggttcg cgcgtcgcaa aacgcgccga agcactcggc ctgcgcgttc tgctcaacga
    10801 cccgccgcgc gccgcacggg aaggtgatac ggggttcgaa tctttggaaa aactgctggc
    10861 ggaagccgac atcgtcacac tccatgtgcc gctgatcaag gaacagccgt ggccgacgct
    10921 taaaatggcg gatgattcat ttttcaaaaa aatgaaacgc ggcgcggtct tcatcaacgc
    10981 ctcgcgcggc aaggttctcg attccgatgc gctgctccac gccaaagaaa acggaatcgt
    11041 atcgcacgcc gcactcgatg tctgggatcc ggaaccgtcc attcgcgccg acgtactggc
    11101 tgccgcaacg attgcaacgc cgcatatcgc cggccactcg tttgaaggca aactgaacgg
    11161 aaccgttcag gtctatcgcg aagcctgccg gttttttaaa accgctccgg tctgggatcc
    11221 tgcaccgctg ctgcctgaac ccgctgtacc ggaactgaag attgattcac gcggcaaaag
    11281 cgaccttgag gtgctgactg aagcagtcaa ggcggtttat gacatcggca cggattgcct
    11341 gaccgaaaaa gatattgagc ggtttgacaa gctgcgtgct cactacggtg tacgacggga
    11401 attcaaaaat acgaccgtat tcctgtccga aacccgtccg gatctgctga aaaaaatcgg
    11461 caaagccgga ttcacccttg cgtagagccg cgcaccagat aacgggtaag acaaagccgg
    11521 cacttcatta gccggctgtt aggtttcagc caccgggtaa gctggctccg gcgtgtgcag
    11581 tgcgatgcgt tctctcttcc acaggcgggg cagcggttcg ccgaatgtac tttcagatgc
    11641 ataaaacacc ttccgaatta cgcatcgatt atgcgtattt tccctggaag gcaacagcat
    11701 tttgcaaggt cttgccttac ataaagtacg gtcagcggta attaaaaagc tgatcaagct
    11761 tataagcctc tccggtggaa gaggaaatgg ctgcaacaat tttatcctga atattgcgcg
    11821 tcaggcgccg gcctttgcga ccctt
//
LOCUS       DAOPZO010000052        11626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_6313_length_11626_cov_5.910207, whole genome shotgun sequence.
ACCESSION   DAOPZO010000052 DAOPZO010000000
VERSION     DAOPZO010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 11626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11626
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6313_length_11626_cov_5.910207"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..2954
                     /locus_tag="PKI68_04830"
     CDS             183..2954
                     /locus_tag="PKI68_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX53050.1"
                     /translation="MFFSPALFAQLPAPIATQATSVLLAQATEMLNEGAAKELLPYLK
                     EILVRLEGNTTDNAQATRAFCLYQIGACQMQIGNYAEAVAAFGAFINAYPADSSASLA
                     GLLTAECYALQRDWPAAEKAVRPLLEDRRFDAKRHLTVWQILAEALYRQQKWKEATVP
                     LLEVFRLAEREEARSNAALMLVVCYAKSDDFENLSRFLPYCGDSVRQDAGLNMALIET
                     GDKRSAAGDYQNALKLYRMVFMKDELLAHYERQMEEIKKFLAQPFVQRIGATRSAYDE
                     NLRVKQMQYDRMAEQLKMIRAGANYDLDIALRKARCYAGLQRSWVAYTIYTGIYTAFP
                     DHELAEDSRFHAFMLLLNMPQEQETALREGRAYLERYPAHKYTDEVSLNLTQLLLTAG
                     RINEARTMGRKALQLSPKHRYMDQVMYLLAFTDFKKQDYESARAAFTGIRSRWPAGVY
                     AEASEYWQAMCCLFMTRYDDAISAFETYLKNPAYPKKSFEEDASYRLGVAFYGKADFA
                     GSEKAFCRFLDRYPGSALRSEAFCMLGDLRGAEGELDAALDFYRKGQAAATNTEQMNY
                     SVFQAARVYELQKNYPAIIGLMEKYTNDRGEESDFAGAGFWIGKAYKAMDQYSKAVST
                     YVDTAVRFGNSPENDHVDLILRELVKEFKSAQGQADQPGFINTLTNALQQAEARHQET
                     LTLRLQTLFASITEGTERERYVSAILKEGNIGKSGALTLRLFAEEAFSRRDYTLVYKV
                     CDHFVSKFESSEMLPDMMNIKLKALVLDAKYPDAMLLAEEIAARFGYQRQVGLTRKLK
                     ADAQRLSKQYEEAIKTYNELFVIREWRGPLTPEALYWIGCCLESQEKTGEAFAYFQRV
                     YVLYEGYTDWSAKAYEGSIRCLEKMGRRDDVRKTLQEMLSKADVAATPEGRRAKAMLE
                     NPASAGGGK"
     gene            2951..3745
                     /locus_tag="PKI68_04835"
     CDS             2951..3745
                     /locus_tag="PKI68_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53051.1"
                     /translation="MKIKSAGAFAGLLLACGLPALFAQADVKALVTYDRNAVVSVVNP
                     AAQADALILAQGTLPFARISRMEFEFGDGLTAQKCESLFKNGAFDPLEKLLADALMPV
                     NSFVRLPGNLDVYLTWQMKVQFWNGHYAEMAQTVDLLKRRNAPSAGLAGIYSALALIE
                     QNRPAEAAQIVSALDNAEAISAPAALFVRARLAMAKKEHREALQMLARIVVLHDKDPE
                     WMPAATLYEGLIYSRTGCPEAAGNIANELIGKYPDGYWSRRAAELK"
     gene            3771..4502
                     /locus_tag="PKI68_04840"
     CDS             3771..4502
                     /locus_tag="PKI68_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNX53052.1"
                     /translation="MKKRIGTVLMMLVLGAAPVWAQTASAVNVMTGNKTATLWQLIVV
                     GGWCMVPLGLCSVAAMYFVIRNILTLRAKNLLRADLQPEIEALLARRDIKGVREVCRN
                     NPSLMTSVLDAGLERISENDFNPAHVMEAIEEAGNEQMVSYMRPINYLSMLGTAAPMM
                     GLLGTVSGIIKAFATISAGQMGKPEMLAGNIGEALVATATGLVIAIPAMMAYSIFKNG
                     FLKNMSTMGRMIGHFMNVYRNSAGR"
     gene            4515..4937
                     /locus_tag="PKI68_04845"
     CDS             4515..4937
                     /locus_tag="PKI68_04845"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX53053.1"
                     /translation="MKFKIPTEEDEEINVMPLIDVVLMLLIFFMVSSHMNTLERVTVS
                     LPVADKAKVPEDARDRQVITVESETGGRVTYYMNLRKMDIKELSAEIVRLHEADENMK
                     VYLRADRQVRHKHIKAVMEACAEAGIADIIFGVVESGS"
     gene            4941..5381
                     /locus_tag="PKI68_04850"
     CDS             4941..5381
                     /locus_tag="PKI68_04850"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX53054.1"
                     /translation="MKAWLEELSNDKMKLQLVPLIDCVFVLLIYFMVCCTLVRPESDL
                     SLTLPGQVAQTKELKMLDEQIVEVLASGSIVLNGKTYSDPSKADMSGLERTLTRYREA
                     SQLAGTTAMITITAEDEAVHERVVDVLNACAGAGITSVTFGRTD"
     gene            5550..8288
                     /locus_tag="PKI68_04855"
     CDS             5550..8288
                     /locus_tag="PKI68_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX53055.1"
                     /translation="MKSSLTFRRLSRLVILLTINSILFTPSAFSAESAVTQSKATSVL
                     IDEVQDLLAAGSFKEAAVYLREILVRIESATDAESQAARASCSYQLGLCLMKTADFAG
                     AAESFKKFISDYPRNEQVPVARFMVLEAYARQNERAQMTAWLNELKTGGAFDELIRFF
                     SDAKNAEFRRNAVLTLVTDYAGQGDLENLRTFLPFCDESVLADIGLNTALMEGGDLLF
                     DKQEYVRALVLYRMVRLKDELMPLYKKQAAALKASLAAASPWVPLEERAHQDEDRQAD
                     QNRLDGLKQTIQFFTDDGYDQDLMVRIAQCYDAMQRYRLSFAAYSYVYNKFPEHRQAE
                     PCRASAFQALMALGDQDEALAAGRDYLARYPQGRFEDEVTVSLMQLYLTRGELTAAGD
                     LGRGALAALPNRRLADQITYLLGFTLLQQQSWPEAFTLFSQVKEKWPQSSCVQDADYW
                     CGMCLLFEGRFAEAIAAFEGYLNNQAYQPARLTDEVTYRLGVAQYGAEAFADSERTFK
                     KFLELYPKSPLVSEAYSMLGDLRGAEGELAPALVFYQKAVETATDAEQDSYAVFQSAR
                     TYELQQRYADTVNLMKAYIARRGAQARLADAGLWIGKACKAQGDRRQALEIYLKTLTD
                     FGNDPVLDGADQVQTQILNDLKDNQNQGDLSFAQARLIDALSRARQKNQTALALRLTA
                     LLARLDGETGRAHYLADLHNEKNLELFSPLPLIVLAEDFAASGNLEGVERLSEAFQTR
                     FSGSEKLPDMLNIEASTCLAAKQYEKTVALTEEVINRLGTDPRAAFSRKLQGDAFRLS
                     GEWVKAVEAYQKVFSIRTGLGSVAPETLYWMGVCKREQGDLEKAFAFFQRVYVLYKGH
                     AEWAAKAYEASADCLQKLGRNAEAVQTRKEMVADPSVQNTPEGLRAAELLRSANQG"
     gene            8292..8774
                     /locus_tag="PKI68_04860"
     CDS             8292..8774
                     /locus_tag="PKI68_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53056.1"
                     /translation="MKRILIIGIVGLSAVCAVSSGAEVPAAVHTPAALPAGKGAGSPE
                     QTAVLNQVLGLLDQSRTDDAAALLEKVPQADAFSPMKEYLQARLAFERGQYTEALQHL
                     AQVENFGGRDSEWLPASVFLEGMVYKKTGQTEAAAFTAQELVLGWPESVWSRRAAELK
                     "
     gene            8800..9531
                     /locus_tag="PKI68_04865"
     CDS             8800..9531
                     /locus_tag="PKI68_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNX53057.1"
                     /translation="MKKRIGTVLMMLVLGAAPVWAQTASAVNVMTGNKTATLWQLIVV
                     GGWCMVPLGLCSVAAMYFVIRNILTLRAKNLLRADLQPEIEALLARRDIKGVREVCRN
                     NPSLMTSVLDAGLERISENDFNPAHVMEAIEEAGNEQMVSYMRPINYLSMLGTAAPMM
                     GLLGTVSGIIKAFATISAGQMGKPEMLAGNIGEALVATATGLVIAIPAMMAYSIFKNG
                     FLKNMSTMGRMIGHFMNVYRNSAGR"
     gene            9544..9966
                     /locus_tag="PKI68_04870"
     CDS             9544..9966
                     /locus_tag="PKI68_04870"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX53058.1"
                     /translation="MKFKIPTEEDEEINVMPLIDVVLMLLIFFMVSSHMNTLERVTVS
                     LPVADKAKVPEDARDRQVITVESETGGRVTYYMNLRKMDIKELSAEIVRLHEADENMK
                     VYLRADRQVRHKHIKAVMEACAEAGIADIIFGVVESGS"
     gene            9970..10410
                     /locus_tag="PKI68_04875"
     CDS             9970..10410
                     /locus_tag="PKI68_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX53059.1"
                     /translation="MKAWLEELSNDKMKLQLVPLIDCVFVLLIYFMVCCTLVRPEADL
                     SLSLPGQVKQTETVVMPDEQVIEVRSDGTIILNDQQYANDSKADTAALEQTLIRYREA
                     SSLLDVQPLITISADKDSVHGRVVDVLNACAGAGIKNVTFANVE"
     gene            10428..>11626
                     /locus_tag="PKI68_04880"
     CDS             10428..>11626
                     /locus_tag="PKI68_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53060.1"
                     /translation="MKKRNLSSFLKNKTRAYSAQAAITTVVVHLLLIGFAGSVVVVHY
                     VQKRNAELIAHTEAKPKLERKKLQAPAARVEQLQQKALTSKLVSKKVSFSNPKFVLPD
                     TGKISSVKTQKLSLPGTDSGRVLKNLSRMPGIGPSRINFFGVRVESEKAVFVIDASAA
                     MLDARTGGPSTYEYIKAELAKIVSEMKPAMLFNIVFYDQQRVFMFRPNLVPASRETVA
                     QLGEWLRAVNGDPALAGLGPEQNNYKAPVSYETAVGSDADGWLLALQAVMEQQPDTIL
                     VLGTGWGHHHISREKANRLLDFAMWELVTENTVSEASALAPDRKVRDDLLKEAVTTIK
                     QEEKIRAAKKTPAGFIRDISQYVEYSKDQVLDHLEAVNKTAYAAHGLSRPDVYYVCLT
                     ESDNLAVA"
BASE COUNT         2605 a         2838 c         3601 g         2582 t
ORIGIN      
        1 cgatctgtat gttcgccatg agcgggcggc ggaattgaag tttgctgaat atttatccgt
       61 ccaggtccag tttaagctgt ttctgactct gccgattgat atataaaagc attatgctgt
      121 ctgcagtcgg tacacggttc cggatgagtt gtcgaagcat cctttgttcg ggatgttttt
      181 ttatgttttt cagtccggct ctgtttgcac agctgccggc tccgattgcg acgcaggcaa
      241 ccagcgtgct gctggcgcag gccacggaaa tgctgaatga aggcgcagca aaggaactgt
      301 tgccgtacct caaagaaatc ctggtccgtc tcgaaggcaa tacgacggat aatgcgcagg
      361 cgacccgcgc tttctgtctg taccagattg gcgcctgtca gatgcagatt ggcaactatg
      421 ccgaggccgt tgcggctttc ggggcgttta tcaatgcgta tcctgccgat tcttccgctt
      481 cgctggccgg gctgctgacg gcagagtgtt atgcgttgca gcgagactgg cctgctgcag
      541 agaaagcggt tcggccgttg ctggaggacc ggcggtttga tgccaaacgc catttgaccg
      601 tttggcagat tctggccgaa gcgctgtacc gccagcagaa gtggaaagaa gcgactgtgc
      661 cgttacttga ggtgttccgt ctggcggaac gtgaggaggc ccgcagtaat gcggcattga
      721 tgctggtggt ctgctatgcc aagagtgatg attttgagaa tctcagccgg tttcttcctt
      781 attgcggcga ttcggtccgg caggatgccg gcttgaatat ggctttgatc gaaaccggtg
      841 ataaacgaag cgccgccgga gattatcaaa acgcattaaa actttaccgg atggttttca
      901 tgaaggatga gctgctggct cattatgagc gccagatgga agaaattaaa aaatttctgg
      961 cgcagccgtt tgtgcagcgg atcggtgcaa cacgcagtgc gtatgatgaa aatctccgtg
     1021 tgaaacagat gcagtatgac cgcatggccg agcagctcaa aatgatcaga gccggggcga
     1081 actatgattt ggatatcgcg ctgagaaagg cccgctgtta tgccggactg cagcgcagct
     1141 gggtggccta tacgatctat accgggattt acacggcatt tcctgatcat gaactggcgg
     1201 aagacagccg gtttcatgcg ttcatgctgc tgctgaatat gccgcaggag caggaaacgg
     1261 cgctgagaga agggcgggcg tatctggagc gttatccggc ccataaatat acggatgagg
     1321 tttctctgaa tctgacgcag cttctgttaa ctgccggcag aatcaatgaa gcccggacaa
     1381 tgggccggaa ggctctgcaa ttgagtccga agcaccgtta tatggaccag gtgatgtatc
     1441 tgctcgcatt tactgatttc aaaaaacagg attatgaatc cgcccgcgcc gcgtttaccg
     1501 gcatacggtc acgctggccg gccggcgtgt atgccgaggc ttctgagtac tggcaggcca
     1561 tgtgctgcct gttcatgacc cggtacgacg atgcgatcag cgcgtttgag acctatctta
     1621 aaaatccggc ctatccaaaa aaatcatttg aagaggatgc ttcttaccgg ctgggcgttg
     1681 cgttttacgg taaggccgat tttgccggtt ccgaaaaagc gttctgccgg ttccttgacc
     1741 ggtatccggg cagtgcgttg cggtcggagg ccttttgcat gctgggcgac cttcgcggag
     1801 cagaaggtga gctggatgcc gcgctggact tttaccgcaa aggacaggct gccgcgacca
     1861 ataccgagca gatgaattat tcagtatttc aggccgccag ggtttatgag ctgcagaaaa
     1921 actatccggc catcatcggg ctgatggaga aatatacgaa tgaccggggc gaagagtctg
     1981 attttgccgg tgcgggtttc tggatcggca aagcgtacaa ggcgatggat cagtacagca
     2041 aagctgtttc cacctatgtg gacaccgccg tccggttcgg caactcgccg gagaacgatc
     2101 atgtggatct gattctgcgc gagctggtca aagaatttaa gagcgcgcag ggacaggcgg
     2161 atcaacccgg atttatcaat acgcttacaa acgctttaca gcaggcggaa gcccggcatc
     2221 aggaaactct gacactccgt ttgcagacgc ttttcgcaag cataacggaa ggaaccgaaa
     2281 gagaacggta cgtcagtgcg atcctgaaag aagggaatat cgggaagtcc ggagcgctta
     2341 cgctgcggct gttcgcggag gaggctttca gccgccggga ctatacgctt gtgtataaag
     2401 tttgtgacca tttcgtcagt aaatttgaat cgtccgaaat gctgccggat atgatgaata
     2461 tcaaactgaa agcgcttgtt ctggatgcga aatatccgga tgcgatgctg ctggctgaag
     2521 agattgctgc gcgtttcggc tatcagcggc aggttgggct gacgcgcaag ctcaaggcgg
     2581 atgctcagcg cctttcaaag cagtatgagg aggcgataaa aacctataac gaactgtttg
     2641 tgattcgcga atggcgcggg ccgctgacgc ccgaggcgct ttactggatc ggatgctgtc
     2701 ttgaatcgca ggaaaaaacc ggcgaagcct tcgcttattt ccagcgggtt tatgtgctgt
     2761 atgaaggata tacggactgg tcggccaaag cctacgaagg cagtatccgg tgtctggaaa
     2821 aaatgggccg gcgtgacgat gtgcggaaga ctcttcagga gatgctgtcg aaagcggatg
     2881 ttgcggcaac gcccgaaggg cggcgggcca aggcaatgct tgagaacccg gcttcggcag
     2941 gaggcggaaa atgaaaataa aatcagccgg agcttttgcg ggattgctgc tggcctgcgg
     3001 tttgcctgcg ctgttcgctc aggcggatgt gaaggcgctt gtcacctatg atcgaaacgc
     3061 tgttgtcagc gttgtaaacc ctgcggcaca ggctgacgca cttatcctgg ctcagggcac
     3121 gctgccgttt gcgcggatca gccggatgga gtttgaattc ggcgacgggt tgactgctca
     3181 gaagtgcgag tcgcttttta aaaacggagc gttcgacccg ctggaaaaac ttttggcgga
     3241 tgcgctgatg ccggttaact cctttgtgcg cctgccggga aatctggatg tatatctgac
     3301 ctggcagatg aaggtgcagt tctggaacgg acactatgcg gagatggctc aaacggttga
     3361 cctgttgaag cggcgcaatg cgccgtcggc cgggctggcc ggaatctatt ctgcacttgc
     3421 gctgattgaa cagaaccggc ccgccgaagc cgcgcagatc gtttccgctt tggataacgc
     3481 agaggcgatt tcggcgccgg cggctttgtt tgtacgcgcc cggctggcta tggcaaagaa
     3541 ggagcaccgt gaagcgttgc agatgctggc ccgtattgtt gtcctgcacg acaaagatcc
     3601 ggagtggatg ccggcggcga cgctttatga aggtctgatt tacagccgga caggctgtcc
     3661 ggaagccgcc ggtaacatag cgaacgaact gatcggaaaa tatccggacg gatactggag
     3721 ccgtcgtgcg gcggagctta aataatcagg aacggatgag ggagggaatg atgaagaaac
     3781 ggatcggaac ggttttgatg atgctggtgc tgggtgcggc tccggtgtgg gcgcagacgg
     3841 cgtcggccgt caatgtgatg acggggaaca agacggcgac gctgtggcag ctgatcgtgg
     3901 tgggcggctg gtgtatggtt ccgctggggc tttgctcggt ggccgcgatg tattttgtga
     3961 tccgcaatat tctgaccctg cgggcgaaga acctgctgcg cgcggatctt cagccggaaa
     4021 tcgaagccct gctggcccgc cgcgacatca aaggcgtgcg cgaagtctgc cggaacaatc
     4081 cgagcctgat gacttccgtg ctggatgcgg gtcttgagcg tatttcggag aacgacttta
     4141 atccggcgca tgtgatggag gccatcgaag aggccggcaa cgaacagatg gtcagctata
     4201 tgcggccgat caactatctc tcgatgctcg gcaccgccgc gccgatgatg gggctgctgg
     4261 ggacggtttc cgggattatt aaagcgtttg cgaccatctc ggccggacag atgggtaaac
     4321 cggagatgct ggccggaaac atcggagaag ctctcgtggc caccgcgacc ggtctggtga
     4381 tcgccattcc ggcgatgatg gcctattcca tcttcaagaa cggctttctc aaaaacatgt
     4441 ccacgatggg ccgtatgatc gggcacttca tgaacgtcta ccggaattca gcgggccggt
     4501 aaaaggcatc ggcaatgaaa tttaaaattc caaccgaaga agatgaagag atcaacgtaa
     4561 tgccgctgat cgacgtggtg ctgatgctgc tgatcttttt catggtgagt tcacacatga
     4621 acacgctgga gcgggtgacg gtttctctgc cggtggcgga caaggcgaaa gtgccggaag
     4681 atgcgcggga ccgtcaggtg ataacggtgg aatcggaaac cggcggcaga gtgacgtact
     4741 atatgaatct gcggaagatg gacattaaag agctgtccgc cgagatcgtc cgtctgcatg
     4801 aagccgacga aaacatgaag gtgtatctgc gggcggaccg tcaggtgaga cacaagcaca
     4861 ttaaagcggt gatggaggcg tgcgcggaag cgggaatcgc cgacattatc ttcggggtgg
     4921 ttgaatccgg gagctgatga atgaaggcct ggctggaaga actgagcaac gacaagatga
     4981 agctgcagct tgtgccgctg atcgactgcg tgtttgtgct gctgatttat ttcatggtgt
     5041 gctgcacgct ggtccgcccg gaatccgatt tgagtctcac gctgccggga caggtggccc
     5101 agacgaaaga actgaaaatg ctcgacgagc agattgttga agtgctggcc agcggatcga
     5161 ttgttctgaa cggaaaaacc tattcagatc cttccaaggc ggatatgagc gggctggagc
     5221 gtacattgac ccggtatcgg gaagcgtctc agctggccgg cacaacggca atgatcacga
     5281 ttaccgctga agatgaagcg gttcatgaac gggtggttga tgtgctgaac gcctgcgccg
     5341 gtgccggtat aaccagtgtg accttcgggc gcacagatta aacaggaagc cgcggttcgt
     5401 gcagttttta caagggcctg gtgcagttgc gaaaagcagc cgtccggtat cgcgacagtc
     5461 cggaccggct ccatttgccg gcatgacact gcaaaatatt tctattcatc ttggacagtc
     5521 ggacggtttc tgttagacat tccgccttta tgaaatcctc tttaaccttc aggcggcttt
     5581 caagactggt cattttgctg accatcaatt ctattctttt tacaccaagc gcattttctg
     5641 cggaatccgc cgttactcaa tcaaaagcca ccagtgtgct gattgacgag gtgcaggatc
     5701 ttcttgccgc cggctcgttt aaggaagcgg ctgtttatct gcgcgaaatt ctggtccgga
     5761 tcgagtcggc aaccgatgcc gaatcgcagg ccgcccgcgc ttcctgcagc tatcagctgg
     5821 gcttgtgtct gatgaaaacg gctgatttcg ccggtgccgc agaatctttc aaaaaattca
     5881 tttcagatta tccgcggaat gaacaggtgc cggttgcccg ctttatggtg ctcgaggcgt
     5941 atgcccggca gaatgagcgc gctcagatga cggcctggct gaatgaactg aagaccggcg
     6001 gagcctttga cgaactgata cggtttttca gcgacgcgaa aaacgccgag ttccgccgca
     6061 atgccgttct tacactggtg acggattatg cggggcaggg tgatctggag aacctccgaa
     6121 cattccttcc gttctgcgac gagtccgtac tggctgatat cggactgaat acagctttga
     6181 tggaaggcgg cgatctgctc ttcgacaagc aggaatatgt tcgcgcgctg gttttgtacc
     6241 ggatggtcag gctgaaagat gaattgatgc ctctgtacaa aaagcaggcg gccgcactga
     6301 aagcttcgct ggcggctgct tcgccctggg ttcctcttga agaacgtgcg caccaggacg
     6361 aagaccggca ggccgatcag aaccggctgg acgggctcaa acagacaatc cagttcttca
     6421 ccgacgacgg ctacgatcag gatctgatgg ttcgcattgc gcaatgctat gacgcgatgc
     6481 agcgctacag actttcattt gccgcataca gctacgttta caataaattc ccggaacacc
     6541 gtcaggcgga gccgtgccgg gcttcggcct tccaggcgct gatggcgctg ggggatcagg
     6601 acgaggcgct tgccgccgga cgggattatc tggcccgcta tccgcaaggc cggttcgaag
     6661 atgaagtgac ggtgagcctc atgcagcttt atctgacgcg cggggaattg acggctgccg
     6721 gcgacctcgg ccggggcgct ctggcggcgc tgccgaaccg ccggctggcg gatcagatca
     6781 cctatctgct gggattcacg ttgcttcagc agcagagctg gcccgaagcg tttacgctgt
     6841 tttctcaggt aaaggaaaag tggccgcaaa gcagctgcgt gcaggatgcc gattactggt
     6901 gcggtatgtg tcttctcttt gaaggccggt ttgccgaagc gattgctgca ttcgaagggt
     6961 acctgaataa tcaggcatat cagcctgcgc gtctgacgga cgaggtcact taccggctcg
     7021 gtgtcgcgca gtacggggcg gaagcttttg ccgattccga acgcacgttc aaaaagtttc
     7081 tggagctcta tccgaagagt ccgctggttt ctgaagctta cagcatgctc ggcgacctgc
     7141 gcggcgctga gggcgagctt gctccggcgc tggtgtttta tcagaaagct gtcgaaacgg
     7201 cgaccgatgc agaacaggac agttatgcgg ttttccagtc ggcccggacg tatgagcttc
     7261 agcagcgtta cgcggacaca gtgaatctga tgaaagccta tattgcccgg cgcggcgctc
     7321 aggcacggct ggccgatgcc ggactttgga tcggcaaagc ctgcaaagcg cagggcgacc
     7381 gccgtcaggc gctggaaatc tatctgaaga cgctgacaga tttcggaaat gatcctgtgc
     7441 ttgacggagc cgatcaggtg cagacgcaga tactgaatga tttaaaagac aaccaaaacc
     7501 agggcgatct tagttttgcc caggcccggc tgattgatgc gctttcgcgt gcccggcaga
     7561 aaaatcaaac tgcgctcgcc ttgcgtctta ccgcgctgct cgcccgcctc gatggcgaaa
     7621 ccggccgcgc gcactatctg gcggatctgc ataacgaaaa aaatctggag ctgttttcgc
     7681 cgctgccgct gattgtgctg gcggaagatt tcgccgcgag cggcaatctg gaaggtgtgg
     7741 agcgtctttc cgaagcattt caaacccgtt tttccgggtc ggaaaaactg ccggatatgc
     7801 tgaatatcga agcgtcaacc tgtctggccg cgaagcagta tgaaaaaaca gttgcgctga
     7861 ccgaagaagt tattaaccgc ctgggtactg atccgcgcgc cgcgttttcc cgtaaactgc
     7921 agggggatgc tttccggctt tccggagagt gggtgaaggc ggttgaggcg tatcaaaaag
     7981 tattttccat acggaccggg ctcggttcgg tggcgccgga aacgctgtac tggatgggcg
     8041 tctgcaagcg tgagcagggt gatctggaaa aagcgttcgc ctttttccag cgcgtctatg
     8101 tgctctataa aggccatgcc gaatgggccg cgaaagccta tgaagccagc gcagactgcc
     8161 tgcagaaact gggccggaat gcggaagcgg ttcaaacccg gaaagaaatg gttgcagacc
     8221 cctctgttca aaacactccc gaagggcttc gcgccgctga gttgttgcgc agcgcaaacc
     8281 aaggataagg tatgaaacgc atactcatca tcggaatagt cggtctgtca gcggtctgcg
     8341 cggtttcttc cggggcggag gttccggcgg ctgttcatac tccggcggcg cttcctgcag
     8401 ggaagggcgc cggttcgccg gagcaaacgg cggtgcttaa tcaggtcctc ggacttctgg
     8461 atcagagtcg aacggacgat gcggccgctt tgctggaaaa agttccgcaa gcagacgcgt
     8521 tttcgccgat gaaggaatat ctgcaggcgc ggctggcatt cgagcgcggg cagtataccg
     8581 aagcgcttca gcatctggcg caggtggaga acttcggcgg acgggactcg gaatggcttc
     8641 cggcgtcagt atttctagag ggaatggttt ataaaaaaac aggacagact gaggcggcgg
     8701 cctttacggc gcaagagctg gttctcggct ggccggaaag cgtctggagc cgtcgtgcgg
     8761 cggagcttaa ataatcagga acggatgagg gagggaatga tgaagaaacg gatcggaacg
     8821 gttttgatga tgctggtgct gggtgcggct ccggtgtggg cgcagacggc gtcggccgtc
     8881 aatgtgatga cggggaacaa gacggcgacg ctgtggcagc tgatcgtggt gggcggctgg
     8941 tgtatggttc cgctggggct ttgctcggtg gccgcgatgt attttgtgat ccgcaatatt
     9001 ctgaccctgc gggcgaagaa cctgctgcgc gcggatcttc agccggaaat cgaagccctg
     9061 ctggcccgcc gcgacatcaa aggcgtgcgc gaagtctgcc ggaacaatcc gagcctgatg
     9121 acttccgtgc tggatgcggg tcttgagcgt atttcggaga acgactttaa tccggcgcat
     9181 gtgatggagg ccatcgaaga ggccggcaac gaacagatgg tcagctatat gcggccgatc
     9241 aactatctct cgatgctcgg caccgccgcg ccgatgatgg ggctgctggg gacggtttcc
     9301 gggattatta aagcgtttgc gaccatctcg gccggacaga tgggtaaacc ggagatgctg
     9361 gccggaaaca tcggagaagc tctcgtggcc accgcgaccg gtctggtgat cgccattccg
     9421 gcgatgatgg cctattccat cttcaagaac ggctttctca aaaacatgtc cacgatgggc
     9481 cgtatgatcg ggcacttcat gaacgtctac cggaattcag cgggccggta aaaggcatcg
     9541 gcaatgaaat ttaaaattcc aaccgaagaa gatgaagaga tcaacgtaat gccgctgatc
     9601 gacgtggtgc tgatgctgct gatctttttc atggtgagtt cacacatgaa cacgctggag
     9661 cgggtgacgg tttctctgcc ggtggcggac aaggcgaaag tgccggaaga tgcgcgggac
     9721 cgtcaggtga taacggtgga atcggaaacc ggcggcagag tgacgtacta tatgaatctg
     9781 cggaagatgg acattaaaga gctgtccgcc gagatcgtcc gtctgcatga agccgacgaa
     9841 aacatgaagg tgtatctgcg ggcggaccgt caggtgagac acaagcacat taaagcggtg
     9901 atggaggcgt gcgcggaagc gggaatcgcc gacattatct tcggggtggt tgaatccggg
     9961 agctgatgaa tgaaggcctg gctggaagaa ctgagcaacg acaagatgaa gctgcagctt
    10021 gtgccgctga tcgactgcgt gtttgtgctg ctgatttatt tcatggtgtg ctgcacgctg
    10081 gtccgcccgg aagctgattt gtctttgagt cttcccggtc aggttaaaca gacggaaacg
    10141 gttgttatgc cggacgagca ggtgattgaa gtccggtctg acggaacaat tattcttaac
    10201 gatcagcagt atgccaatga ttctaaagcg gataccgccg cgcttgagca gacgttgatc
    10261 cggtaccgcg aagcttcttc tctgctggat gtgcagcctt tgattaccat ctctgccgat
    10321 aaagattcgg ttcacgggcg ggtggttgat gtgctgaatg cctgcgccgg tgccgggatt
    10381 aaaaacgtaa cttttgcaaa tgtggaatag gccgggcgtt acacctcatg aaaaagcgaa
    10441 acctcagttc gttcctgaaa aataaaaccc gggcgtattc cgctcaggcg gcgattacga
    10501 cggttgttgt gcatctgctg ctgatcggct tcgcgggctc tgtcgttgtc gttcattatg
    10561 tgcagaaacg gaatgctgaa ctgattgctc atacggaagc caagcccaag ctggagcgca
    10621 aaaaactgca ggcgccggca gcgcgggtcg agcagcttca gcagaaggcg ctgacctcta
    10681 agcttgtcag caagaaagtt tcattctcca acccgaagtt tgttcttcct gataccggaa
    10741 aaatcagctc ggtgaaaaca cagaagctca gcctgcccgg taccgattcc ggacgtgttc
    10801 tgaaaaacct ttctcgcatg cccggcatcg ggccgtcgcg cattaatttt ttcggagtcc
    10861 gggtcgagag cgaaaaggcg gtgtttgtaa tcgatgcttc ggcggctatg ctggacgccc
    10921 ggacgggcgg tccctcaacg tatgaatata taaaagccga gctggcgaaa attgtttcag
    10981 aaatgaaacc cgctatgctg tttaatatcg ttttttacga tcaacagcgt gttttcatgt
    11041 tccgtccgaa tctcgttccg gcttcccgag aaaccgttgc tcagctgggc gaatggctgc
    11101 gagcggtgaa cggtgatccg gcactggccg ggctgggccc agagcagaat aattataagg
    11161 ctccggttag ttatgaaacg gctgtcggtt ctgatgctga cggatggctg ctggcgctcc
    11221 aggcagtcat ggagcagcag cccgatacaa tactggtgct gggaaccggc tgggggcatc
    11281 atcacatcag tcgggaaaag gcgaaccgtc ttcttgattt cgcaatgtgg gagctggtga
    11341 cggaaaatac tgtcagtgag gcttctgctt tggcgccgga tcgtaaggtc agagatgatc
    11401 tgctcaaaga ggcggttaca accattaagc aggaagaaaa aatccgcgcc gcaaaaaaga
    11461 ctccggcggg atttatccgg gatatctcgc agtatgtaga gtattccaag gatcaggtgc
    11521 tggatcattt agaggcggtt aacaaaacgg catatgctgc gcacgggctg tccaggccgg
    11581 acgtatatta tgtttgtctg accgagtccg ataacctggc ggttgc
//
LOCUS       DAOPZO010000053        11420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_6483_length_11420_cov_8.248658, whole genome shotgun sequence.
ACCESSION   DAOPZO010000053 DAOPZO010000000
VERSION     DAOPZO010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 11420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11420
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6483_length_11420_cov_8.248658"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="PKI68_04885"
     CDS             complement(<1..1160)
                     /locus_tag="PKI68_04885"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNX53061.1"
                     /translation="MKEIKIGILGFGTVGAGVVEGILKNGELMAHRTGIFPKIGKIAD
                     LDIETDRGVKVDPSWLTTDALSVINDPEIDVIVELIGGLKVAKDFVLRALKNGKPVVT
                     ANKALLAEAGRELFEASAKSGAGLYFEASVGGGIPVLRALRDGLVGNRIKSMYGILNG
                     TCNYILTRMEREGLDFDTVLADAQKLGYAETPPDLDIDGIDTAHKAVVLASLAYGAPV
                     PMDAFTIEGIRGLSHREIEYAGELGYRIKLLAVIREVNGEVEMRVEPSFVPKKHLLAS
                     VNDAFNAVFVEGDVVGSTLYYGRGAGRLPTASAVIGDIMEAAAGKGGCSNWLLKHTAL
                     PVRDARDISIRCYLRLSLKDQPDVIAQVAHVLGKNKISIASIMQKEQNAGEQV"
     gene            1307..2719
                     /locus_tag="PKI68_04890"
     CDS             1307..2719
                     /locus_tag="PKI68_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNX53062.1"
                     /translation="MKINPSPSYSIDLTVKSTLDRAALEKLIIAAEGVPALSEGVTSP
                     FRFVVNARSSGHQQEIIRALDAAEGTCVLSADEVTFRSHIGGKISVTSKLDLAAKGAL
                     AVAYTPGVGRICMSIHEDVARADALTVKNNMIAVVSDGTAVLGLGDIGPEAAMPVMEG
                     KAMLFKEFGGVDAFPICLNTKDPEEIIRTVKLLGPTFGGINLEDISAPRCFEIEERLK
                     AEMDIPVFHDDQHGTAIVVLAALYNALRIVDKKIDTIKVVVCGTGAAGTACADILMRA
                     GVKNLIGYDIKGAVYKGRPGDSLPLQAFAERTNAEGIRAPLCEGIKGADLFLGVSAPG
                     CITAADVKNMAKDPIVFAMANPVPEIMPELAKPYARIMATGRSDYPNQINNVLCFPGI
                     FKGALRCRASRISEGMKLAAARAIADLVSAGELCEEFIIPSVFDRRVADAVANEVERV
                     AHAEGLAREVIDSKTFYKLR"
     gene            complement(2771..4231)
                     /locus_tag="PKI68_04895"
     CDS             complement(2771..4231)
                     /locus_tag="PKI68_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HNX53063.1"
                     /translation="MRAVLYTGGKRPWAEVLGERPWALLPVGSRPLLSYWLELCIDLG
                     ISDVQIILGRDAEYVEMFCGNGEKWGLNINYSFVRAEDGPQTYLSRDPERWSDGLLYI
                     GEAVFPRRRADFTPEKLNTLRDGCFIRDGENCAFFISHQRESVEHFIRTGHCSFMHCA
                     DPCAGTEICRTPDACGIDFTRIRDISHYYRLNMEIVRNEMSRYLSSGYSSRDGASIGY
                     NVITPPSVTFTPPLAIGNDCRIGAISVIGPGTVISDHVLIDRQCEISDSIILSDTYVG
                     RNLEIKGKIVAGNRIVDPEDGTCLEIADPWLVAQTRPRSYMRDFLRGIFGWEFALLLA
                     VLQFIPFVLLYSVIRLLGRGYFVGRKCRGIGGTEIRVAQFVSATSVPCFLLMVFYGAS
                     LDRFPQLLSVLTGKLWLCGQVPKTVNGDDFKETHRYFPAVFSYCDAFIDIDKQMDALY
                     YAHTRSLAADLRILRHALLHRLIEGESTPGTITQAV"
     gene            complement(4250..4594)
                     /locus_tag="PKI68_04900"
     CDS             complement(4250..4594)
                     /locus_tag="PKI68_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNX53064.1"
                     /translation="MSVACTKEQGIGIITLSGPLTAATVEAFREQLAAWQSGETDVKN
                     FVFDLSAVDFMDSAGLGSLIAALKRITERGGDMKLACLQKKPRMVFEITRAYKVFEIY
                     DTVEEAAKAAKQ"
     gene            complement(4616..5350)
                     /locus_tag="PKI68_04905"
     CDS             complement(4616..5350)
                     /locus_tag="PKI68_04905"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNX53065.1"
                     /translation="MNESVYQARRELFKTLDLPVSKADIKRLRWRQRSRLLVWEWALS
                     SLLVIKRLMDVVCSLMAIILLIPLFVVVSVLIVLEDGWPVLYVQQRVGINGRIFNFYK
                     FRSMRRDADRLKDQLMAQNESADGVIFKMKKDPRVTRIGRILRRFSIDEAPQFFNVLS
                     GDLSLVGPRPPIPSEVAQYTLEERKRLHVKPGLTCLWQIGGRSEIPFEQQVRLDMEYI
                     HSRGLWQDIRIMLKTVPAVLFGRGAY"
     gene            complement(5350..6981)
                     /locus_tag="PKI68_04910"
     CDS             complement(5350..6981)
                     /locus_tag="PKI68_04910"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX53066.1"
                     /translation="MSTDTADDIRTFPLSASRPDLGGKVLVVDDERPNRLYLKKLLTS
                     YGCEVIEAADGPSALELLHRERPDLILADVVMPGMDGFELCGAIKKLTATRDVPVIMV
                     TAKGQIEDLKKGFDLGAMDYIRKPFDARELVLRVGNALALKRSNDALAHWKRRVSHEL
                     ELAGKIQQSMFSTEPLFTGPFEIRIKYRSSMDVGGDAFDVITLPDGRCCVYVADVSGH
                     GVAPAMISSMLKATATELIRTFYERGPAFICNELHVRLRSTLNNPSYYATMLLAFYEP
                     ETGRWICMNCGHPDPLLICEGKVVSFPAGGGGVPVGMPLGPVRPFAEEDQTVLESCPG
                     MNILLYTDGLSEALHRVTGEECGAEGLEKLFTRVVADPFAVNRPSALLDLIEDEGYSI
                     GHDDCTAITIRMTDPSTVLLEGTIAVDVQAVSERTEEMERLLLERTDEDLAARVRLVA
                     IEHAMNVVEHSRLGAHETMWTQLILDEHGCRLVFTDPGREWNISGAGVTEPGLDEYAE
                     GGRGLLITNVAADCVERYRRDSQNVVSYVFRRQTD"
     gene            complement(6978..7337)
                     /locus_tag="PKI68_04915"
     CDS             complement(6978..7337)
                     /locus_tag="PKI68_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNX53067.1"
                     /translation="MAISRAIETVRKLVPDLDGRISQFEVETFEMDDELIAAFCEELE
                     RMTADLQTGLDKPDTELIRVAAHSIKGMGGTMGLPEISVLAQEIELTLRGGQMDRCSS
                     LCKALISWSSDFVAGNQ"
     gene            7478..9622
                     /locus_tag="PKI68_04920"
     CDS             7478..9622
                     /locus_tag="PKI68_04920"
                     /inference="COORDINATES: protein motif:HMM:NF016611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX53068.1"
                     /translation="MQNIFTDYPYVPWKQNWRFFAGPEDRAPFITGKDSGSITVPEGN
                     WTLQLADPAAPCTIRLFCSGLSISARKRPEKNRIDVLLTESGLLYGTVSGLPVPRLTA
                     SAEIIKDKGFDWIQNGRITTLLLQQDNRFALVCGELTVERALAKAEAAFDLNFAALAQ
                     SETDERKNVSRLFSVNQRHNPPVALAAENLTARLRERTGALHGLWSAAEGFTAETFSL
                     NELYPLVRAWNLIDPAVALELVQTALSLQQSSGGFPAWAESGGLTSPAAPWPLIAQSF
                     ELAWQSGREPALLRKYLPALRKYIQWAARRFDPQRDRIPAWQNEQEIFVPGSFERGKA
                     TPELTVMLIAEIEAVLRLCKENSHAETASESLTDERDQLARTLYTAFWNPEAKAFSNV
                     WKAGHYLHEPSFGSFMPLFWRGLDHEKQTALLENFEETHGFPGHQEPSSWKQDEIDDT
                     AHLPAIHQFMAFEALRAADSTRLLLMFFVRRARESFATWFERESIEAARGTATGAPAY
                     AFGPVTAALILTTQEEFEREAAKAPSAGQRILHWGHRRQISKADLRVVAVFLLAILIA
                     HLAYTLPHSLNTDARVAEAALNYQQGRYIETIKICRRYPDNALSQFLQANLLMIAGRP
                     DQAEELYRKALTQKPESPSALFGLALTLQMNGKFKEAEKRYIDFIDIHEAHHPEEAEL
                     ADQFMMLTREKFSKPPNWRRVYAIPMMNDLGL"
     gene            9662..9991
                     /locus_tag="PKI68_04925"
     CDS             9662..9991
                     /locus_tag="PKI68_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53069.1"
                     /translation="MKTFLKTLLPLAALLLPAGCFRNDIHTETFQINQLRTQAGAAII
                     AQSFQQMSGIQEMRPDFAKHTLTVVYNAKDTHLKNIEYAIVKAGFDLPHWPADPADKA
                     KLPEELR"
     gene            9991..>11420
                     /locus_tag="PKI68_04930"
     CDS             9991..>11420
                     /locus_tag="PKI68_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HNX53070.1"
                     /translation="MKLTLTARPLATQAREAWIVFVQNKKPLFNGHDEMAKRIGSQIK
                     KTGYSGDYAQTLVFQTADGDVILAGLGDSKHFRMEYLERAASAAVRAGKNAGLKNFAT
                     VTDAGIGKISDAAFVHAVARGAAMGTYSFDTFKTGGKKRAGELSLTFAGPCENSAACR
                     KALAEAELESKALLSTADLANLPGNEATPQKIFSWAQKMAKENKLAFSVLSKAELKKK
                     GCNAILAVAAGSAQDARIITLKYKGTNPKAKPVVIIGKTITFDSGGISLKPGKGMEWM
                     RYDKSGGMAVLAAMQMVARLKPETPVIGMLAVAENMPGGRATRPGDIVKSHAGKTIEI
                     LNTDAEGRLVLADALSLALQHKPAAIVDLATLTGAVVTALGHNAAAVVGNNHKLNATL
                     IKTADAAGERLWELPLWPENMDDMKGTFADLQNMSKSGTAGTSNGAAFLANFVPETIP
                     WAHLDIAGTAWEESSKPWMDPGVTLFG"
BASE COUNT         2618 a         3508 c         3081 g         2213 t
ORIGIN      
        1 acctgttcgc ccgcgttctg ttctttctgc atgatcgacg cgatgctgat tttgtttttg
       61 ccgagtacgt gcgcgacctg tgcaatcaca tccggctggt ctttcagcga cagccgcaga
      121 tagcagcgga tcgaaatatc cctcgcatcg cgcaccggca gcgcggtgtg cttcaacagc
      181 cagttactgc atccgccctt gcccgccgcc gcttccataa tatcgccgat caccgcgctg
      241 gcggtcggca gacgtccggc tccgcgcccg taatagagcg tactgccgac gacgtcgcct
      301 tcaacgaaaa cggcattgaa cgcatcgttg actgaagcca gcaggtgctt tttcggcacg
      361 aaagaaggct cgacgcgcat ttcgacttcg ccgttcactt cgcggatgac agccagcagt
      421 ttgatgcggt agcccagttc gccggcatat tcaatttcgc ggtgagacag tccgcggatg
      481 ccttcgatcg tgaacgcatc catcggaacc ggtgcaccgt aggccagcga cgccagcacg
      541 accgctttgt gggcggtgtc gatgccgtca atgtcgagat ccggcggagt ttcggcatag
      601 cccagtttct gggcatcggc cagcaccgta tcaaagtcga gtccttcgcg ctccatgcgg
      661 gtcaggatgt agttgcaggt gccgtttaaa atcccgtaca tgctcttgat ccggttgccg
      721 accagtccgt cgcgcagagc gcgcagtacc ggaatgccgc cgccgacgct ggcttcaaaa
      781 tagagtcccg ccccgctctt cgcggaagct tcaaacagct cgcggcccgc ttcggccagc
      841 agcgctttat tggcggtgac caccggcttg ccgtttttca gcgcgcgcag cacgaagtcc
      901 ttggcgactt tcagcccgcc gatcagctcg acaatcacat caatttcggg gtcgttgatc
      961 acggaaagcg cgtcggtcgt cagccaggac ggatcaacct ttacgccccg gtcggtctca
     1021 atgtcgaggt cggcgatttt cccgattttc gggaaaatac cggtacgatg cgccatcagt
     1081 tcgccgtttt tgagaatccc ctcaacgacg cccgcgccga ccgtgccgaa ccccagaata
     1141 cctatcttta tttccttcat aaaaatcccc gtttatggag cgcagacttt accccaaacc
     1201 cgttgaagct caaggcgaaa gcctcggttt ccattttttg aacagttgaa acttgcgcgg
     1261 cagaagcgga tcggtacgat gggcggctac tttgaggagt tgccccatga aaatcaatcc
     1321 gtcgcccagc tacagtatcg acctgaccgt taaatcaacc cttgaccgcg cggcgctcga
     1381 aaaactcatt attgccgccg aaggcgtacc cgctctttcc gaaggcgtca ccagcccgtt
     1441 ccgctttgtc gtcaacgccc gcagcagcgg ccaccagcag gaaatcatcc gggcgctgga
     1501 tgccgccgaa ggaacctgcg ttctttctgc ggatgaggtg acattccgtt cgcacatcgg
     1561 cggaaaaatt tcagtaacca gcaagctcga tctcgccgcc aaaggcgcgc tggccgttgc
     1621 ctacacgccg ggcgtcggcc gcatctgcat gtcgatccat gaggatgtcg cacgcgccga
     1681 tgcactgacc gttaaaaaca atatgatcgc agtcgtttcc gacggcacag cggtgctcgg
     1741 tctcggcgac atcggcccgg aagccgccat gccggtgatg gaaggtaagg cgatgctctt
     1801 caaagaattc ggcggcgtgg atgcgtttcc gatctgcctg aacaccaaag accccgaaga
     1861 gatcatccgg acggttaaac tgctcggacc gacgttcggc ggcatcaacc tggaagatat
     1921 ctccgctccg cgctgttttg aaattgaaga acggctgaaa gcggaaatgg atattccggt
     1981 tttccacgac gaccagcacg gtaccgccat tgttgtactg gcggcactgt ataacgctct
     2041 caggattgtt gataaaaaaa tcgacacaat caaagtggtc gtctgcggaa ccggtgcggc
     2101 aggaaccgcc tgcgccgaca ttttgatgcg cgccggagtc aaaaacctga tcgggtacga
     2161 cattaaaggc gcggtttata aaggccgccc gggcgacagc ctgccgcttc aggcgtttgc
     2221 agagcgaacc aatgccgagg gaatccgcgc tccgctgtgc gaaggaatca aaggagccga
     2281 cctgttcctc ggcgtctcgg caccgggctg catcaccgcc gccgatgtga aaaacatggc
     2341 gaaagatccg attgttttcg cgatggccaa cccggttccg gaaatcatgc ccgaactcgc
     2401 caaaccttac gcccgtatta tggcaaccgg acgctcggac tatccgaacc agatcaacaa
     2461 cgtgctctgc ttccccggca ttttcaaagg cgcgctgcgc tgtcgcgcaa gccgtatcag
     2521 cgaaggcatg aaactggccg cagcccgcgc gatcgccgac ctggtttccg ccggcgaact
     2581 ctgcgaggag ttcattattc cgtcggtttt tgaccgtcgc gtggcggatg cggtcgccaa
     2641 tgaagtggag cgcgtcgcac acgccgaggg acttgcccgc gaagtcatcg acagcaaaac
     2701 gttctataaa ctccgctgag ttcccgggaa ttgaaaacct gcggcgccgg aagcagtgca
     2761 gatcaggaaa tcagacggct tgcgtgatgg tgcccggcgt cgattcgcct tcgataaggc
     2821 ggtgcaggag cgcgtggcgc agaatacgca ggtcggcagc cagcgagcgg gtgtgggcgt
     2881 agtacagcgc atccatctgt ttatcaatgt cgatgaatgc gtcgcaatag ctgaaaacag
     2941 cggggaagta acggtgtgtc tccttgaaat cgtccccgtt gacagttttc ggaacctgcc
     3001 cgcaaagcca gagctttccg gtaagaacac tgagcagctg agggaaacga tccagcgaag
     3061 cgccgtagaa gaccatcagc agaaaacacg gaacgctggt ggctgacaca aactgcgcaa
     3121 cacggatctc ggttccgcca atcccccggc atttacggcc gacaaagtat ccgcggccca
     3181 ggaggcggat cacgctgtac agcaaaacaa acgggataaa ctgcagaacg gcaagcagca
     3241 gcgcaaactc ccagccgaag ataccgcgca gaaagtcgcg catatagctg cgcggacggg
     3301 tctgggccac aagccacgga tctgcgattt caaggcaggt gccgtcttcc ggatcgacga
     3361 tgcggttgcc ggcgacgatt ttccctttga tctcaaggtt gcggccgacg tatgtgtcag
     3421 aaagaatgat gctgtcagaa atttcgcact gccggtcaat gagtacatgg tcagagataa
     3481 ccgtcccggg gccgatgaca gagatggccc cgatgcggca gtcgttaccg atcgccagcg
     3541 gcggtgtaaa cgtgacggaa ggcggcgtga tgacgttgta gccgatggaa gcgccgtcgc
     3601 gggaggaata accgctggag agatagcggc tcatttcgtt ccggacgatt tccatattca
     3661 ggcggtagta gtggctgatg tcgcgaatgc gggtgaagtc gatcccgcag gcatccggag
     3721 tgcggcagat ttcggttccg gcgcacggat cagcacagtg catgaaactg cagtgtccgg
     3781 tgcgaatgaa atgctcaacc gattcacgct gatgactgat gaaaaaagcg cagttttcac
     3841 cgtcgcggat aaaacagccg tcgcgaagtg tgttaagttt ttccggcgtg aaatcagccc
     3901 ggcggcgcgg aaaaaccgct tcgccgatgt aaagcagccc gtcactccac cgttccggat
     3961 cacgcgaaag ataggtctgc ggaccgtcct cggcgcggac gaagctgtaa ttaatgttca
     4021 gcccccattt ttcgccgttg ccgcagaaca tttcgacata ttcagcgtcc cggccaagga
     4081 tgatctgaac atcgctgata ccgaggtcga tgcaaagttc gagccagtag ctcagtaaag
     4141 gacggctgcc gaccggcagc agagcccagg gacgttcccc gagcacttca gcccacggcc
     4201 tttttccgcc tgtatagagt actgctctca tcacgatgcc gaacggcttt tactgtttag
     4261 ctgctttagc ggcttcttcg acggtgtcgt agatttcaaa caccttgtag gcgcgggtaa
     4321 tttcgaacac catgcgcggt ttcttctgaa gacaggccag cttcatgtcg ccgccgcgtt
     4381 cggtaatgcg tttgagcgcc gcgatgagcg agccaagacc tgcgctgtcc ataaagtcca
     4441 cggcggacag gtcaaaaaca aagtttttga catccgtttc accggactgc cacgcggcga
     4501 gctgctcgcg aaaggcttca acggtggcgg cggtcagcgg cccggacagc gtaatgatcc
     4561 cgattccctg ttcttttgtg catgctacac tcatgatatc tctcctgttc tataactaat
     4621 aagccccgcg tccgaagaga acggcgggaa cggtttttaa cataatccgg atgtcctgcc
     4681 agaggccccg gctgtgaata tattccatat ccaggcgcac ctgctgctca aacggaattt
     4741 cagaacgtcc gccgatctgc caaagacagg tcaggcccgg tttcacatga aggcgtttgc
     4801 gctcttcaag cgtgtactgg gccacttcgg aaggaatcgg cgggcgcgga ccgaccagtg
     4861 acaggtcgcc ggacagcaca ttgaaaaact gcggcgcctc gtcgatgctg aaccggcgaa
     4921 ggatgcgccc tatccgcgta acacgcggat cttttttcat tttgaaaatc acaccgtcgg
     4981 ccgattcatt ctgcgccatc agctgatctt taagccggtc ggcatcgcgg cgcatggagc
     5041 ggaatttata gaaattaaaa atacgtccgt taataccgac acgctgctgg acataaagaa
     5101 ccggccagcc gtcctcgagc acaatcagaa ccgacacgac cacgaagagc ggtataagca
     5161 gaatgatggc catcagactg cacacaacat ccatgagccg tttaatcaca agcaaactgc
     5221 tcagcgccca ttcccaaacc agcagacggc tgcgctgccg ccagcgcagg cgtttaatgt
     5281 cggccttgga taccggcagg tcgagcgttt taaacagctc ccgccgggcc tgatatacgg
     5341 attcattcat cagtcggtct gcctcctgaa cacgtaactt acaacattct gtgaatcgcg
     5401 tctataacgc tctacacagt cggcggcaac attggtaatc agcagcccgc gcccgccttc
     5461 tgcatattca tcaaggccgg gctctgttac gcctgcaccg gaaatattcc attcgcggcc
     5521 gggatctgta aataccagcc ggcagccgtg ttcgtccaga atgagctgcg tccacatcgt
     5581 ctcgtgcgca ccgagccggc tgtgctcgac tacgttcatc gcatgttcaa tggcgaccag
     5641 ccggacgcgc gccgcaagat cctcatcggt ccgctccagc agcagacgct ccatctcttc
     5701 cgtgcgttca gagacagcct gcacatcaac agcgattgtt ccttccagga gcacagtgga
     5761 cggatcggtc atgcggatgg taatggccgt acaatcgtca tgaccgattg agtacccctc
     5821 atcttcaatg agatcaagca atgcggaggg acgatttaca gcaaaaggat cggcgacaac
     5881 acgtgtaaaa agtttctcca gcccttccgc accgcactct tcaccggtaa cgcggtgcag
     5941 cgcttcacta aggccgtccg tataaagcag aatattcatg ccggggcatg attccagaac
     6001 cgtctgatcc tcttcagcaa acggccggac cggccccagc ggcataccga ccggaacgcc
     6061 gccgcctccg gcaggaaacg aaacgacctt tccttcgcaa atcagcagcg gatcgggatg
     6121 cccgcagttc atacagatcc accggccggt ttccggctcg taaaacgcca gcagcatggt
     6181 ggcgtaatac gatggattgt tgagggtgga acgcaggcgc acatgcagct cgttgcagat
     6241 aaaggcgggg ccgcgttcat aaaacgtccg gatcagctcg gtggccgtcg ctttgagcat
     6301 cgaagaaatc atcgccgggg caaccccgtg ccccgacaca tcggccacat agacacagca
     6361 gcgcccgtcc ggcagagtaa tgacatcaaa cgcatcaccg ccgacatcca tcgaagagcg
     6421 gtatttgatg cggatttcaa acggccccgt aaaaagcggt tccgtggaaa acatcgattg
     6481 ctggatcttc ccggcaagct ccagctcatg tgatacgcgc cgtttccagt gggccagcgc
     6541 atcgttgctg cgtttcagtg ccagcgcatt accgactcgc agcaccagct cgcgcgcatc
     6601 gaagggtttg cggatatagt ccatcgcccc gagatcgaat ccttttttca ggtcttcaat
     6661 ctgccccttg gccgtcacca taattacagg cacatcgcgg gtcgccgtga gttttttgat
     6721 tgcgccgcaa agttcaaagc cgtccattcc gggcataacg acgtcggcca gaatcagatc
     6781 aggtctttcg cggtgcagca gttcgagcgc cgaaggaccg tcggcggctt cgatgacttc
     6841 gcagccatat gaggtgagaa gttttttaag ataaagccgg ttgggacgtt catcatccac
     6901 caccagcact ttaccgccca gatcgggccg ggaagcgctt aacggaaaag tacggatatc
     6961 gtctgccgtg tctgtactca ttgattcccg gcaacaaagt cactggacca gctgatcagc
     7021 gccttgcaaa gcgacgagca gcgatccatc tgtccgccgc gaagtgtcag ctcaatttcc
     7081 tgcgccagca cggaaatttc cggcaatccc atggttccgc ccattccttt gatggaatgt
     7141 gccgccacgc ggatcagttc agtatcaggc ttgtccagac cggtttgcag gtcggcggtc
     7201 attcgctcaa gttcttcgca gaaggcggca atcagctcgt catccatctc gaaggtttcc
     7261 acctcaaact gactgatccg tccgtcgaga tcgggaacca gtttacgaac tgtctctatt
     7321 gcacgtgaaa tcgccataag tccgttcctt ccattcgtag aatgcaggcg atcataacca
     7381 tttaaaagcc gtacgcgcaa gcctttcgct cggctgcgaa atcaaaagca aggtgttgaa
     7441 gaagtctgcc ggttgggaca ctatagcggc cggctgaatg caaaatatct tcacagatta
     7501 tccatacgtt ccatggaagc agaactggcg gttcttcgcc ggtcctgagg atcgtgcgcc
     7561 gtttattacc ggcaaggatt ccggatccat cactgtcccg gaaggaaact ggactctcca
     7621 gctggctgac ccggcggcac cctgcacaat ccggctgttc tgctccggtc tttccatcag
     7681 cgcccgcaaa cggccggaaa aaaacaggat cgacgtcctg ctgacggaat ccggactgct
     7741 ttacggcacg gtatccggcc tgccggtgcc gcgactgacc gcctcggcag aaatcataaa
     7801 agacaaagga ttcgactgga tacaaaacgg ccggatcacc accttgcttc tgcagcagga
     7861 caaccggttt gcgctggtct gcggcgaact caccgtggaa cgggcgcttg caaaagccga
     7921 agccgcgttc gatttaaatt tcgccgcgct cgctcagagc gaaacggacg agcgtaaaaa
     7981 tgtcagccgg cttttcagcg taaaccagcg ccataatccg cccgtcgcgc tcgcagcgga
     8041 aaacctgacg gcccggctgc gcgaacggac cggcgcactc catgggctct ggagcgccgc
     8101 cgaaggcttc accgccgaaa ccttctcgct caatgaactt tatccgctcg ttcgcgcatg
     8161 gaatctgatt gatccggcgg tcgcactgga actggtgcag actgcgcttt cacttcagca
     8221 aagcagcggc ggatttcccg catgggccga atccggcggg ctgacctcgc ccgccgcgcc
     8281 ttggccgttg atcgcccagt ctttcgagct ggcctggcag agcggccgcg aaccggcact
     8341 gctccggaaa tatctgcccg ctctgcggaa atatattcag tgggctgcgc gccgcttcga
     8401 tccgcagcgc gaccggattc ccgcctggca gaacgaacag gagattttcg ttccgggaag
     8461 tttcgaacgc ggcaaagcaa cccccgaact caccgtcatg ctgattgcgg aaattgaagc
     8521 ggtactgcgc ctctgcaaag aaaattcaca tgccgaaacc gcttctgaat cgctcacaga
     8581 tgaacgggat cagctggccc gcactcttta cacggcattc tggaaccctg aagccaaagc
     8641 cttttccaat gtctggaagg ccggacatta cctgcacgaa ccgtccttcg gttcctttat
     8701 gccgctgttc tggcggggac tcgaccatga aaagcagact gcactgcttg aaaatttcga
     8761 agaaacacat ggattccccg gacatcaaga accctcatcc tggaaacagg acgaaatcga
     8821 cgataccgcc cacctgcccg ccatccacca gttcatggcc tttgaagcgc tccgggccgc
     8881 cgacagcacc cgcctcctgc tcatgttctt cgtgcgccgc gcacgggaaa gcttcgccac
     8941 atggttcgaa cgcgaaagca tcgaagccgc gcgcggcaca gcaaccggcg cgccggccta
     9001 cgcgttcggc cctgtcacag cagcgctcat tcttacaact caggaggaat tcgaacgcga
     9061 agcggcaaaa gcaccttcgg ccgggcagcg gattctgcac tggggccacc ggagacaaat
     9121 cagcaaggcc gacctgcgcg tcgtagccgt cttcctgctc gccatcctga ttgcgcatct
     9181 tgcctacaca ctgccgcaca gtctaaacac agacgcccgc gttgccgaag ccgcactgaa
     9241 ctaccagcag ggccgctaca tcgaaacaat caaaatctgc cgccgctatc cggacaacgc
     9301 cctctcgcag tttctgcagg ccaatctgct gatgattgcc ggccggcccg atcaggcgga
     9361 agagctttac cgcaaagcgc tcacacagaa acccgaaagt ccgtccgcac tgttcggtct
     9421 ggcgctcacg cttcagatga acggaaaatt caaagaagcg gaaaaacgct acatcgactt
     9481 catcgacatc cacgaagcgc accacccgga agaggctgaa ctggcggatc agttcatgat
     9541 gctgacccgc gaaaaattca gcaaaccgcc gaactggcgg cgggtttatg ccataccgat
     9601 gatgaacgac ctcggattat gaagtttacc cgcgccctgc ttccgttgta atttccgcgt
     9661 tatgaaaaca tttcttaaaa cactgcttcc gctcgccgcc ctgcttttac cggccgggtg
     9721 cttccgtaac gacatccata cggaaacatt tcaaatcaac cagctccgca cacaggccgg
     9781 cgctgcaatc attgcccagt cctttcagca gatgtccggc attcaggaaa tgcgcccgga
     9841 tttcgcgaaa cacaccctca ccgtggtcta caacgccaaa gacactcacc tcaaaaatat
     9901 cgagtatgca atcgtcaaag ccggcttcga cctgccgcac tggccggccg atcccgctga
     9961 caaagccaaa ctgccagagg aactgcgctg atgaaactga ccctgaccgc ccggccgctc
    10021 gcaacccagg cccgtgaagc ctggattgtt tttgtgcaga acaaaaagcc gctgttcaac
    10081 ggccatgacg agatggcaaa gcggatcggc agtcagatca aaaaaaccgg ctacagcggc
    10141 gactacgccc agacgctcgt tttccagaca gcagacggcg acgtcatcct cgccggactc
    10201 ggcgacagta aacacttccg catggaatac ctcgaacgcg cggcctccgc cgccgtgcgc
    10261 gccggcaaaa acgccggact caaaaacttc gccactgtca ccgatgccgg aatcggaaaa
    10321 atatccgacg cggccttcgt acacgccgtc gcccgcggtg ccgcgatggg aacctacagc
    10381 ttcgacacct tcaaaaccgg cggaaaaaag cgcgccggag aactatcgct gacctttgcc
    10441 gggccgtgcg aaaactccgc cgcctgccgc aaagcactgg ccgaagcgga actggaaagc
    10501 aaagcgctgc tctccaccgc cgacctcgcc aacctgcccg gcaacgaagc cacgccgcag
    10561 aagattttca gctgggcgca gaaaatggcc aaggaaaaca aactggcctt ttccgtactc
    10621 agcaaagccg agctgaagaa gaaaggttgc aacgccatcc tcgccgtcgc ggcgggcagc
    10681 gcgcaggacg cccggatcat cacgctcaaa tacaaaggga ccaatccaaa agccaaaccg
    10741 gttgtcatca tcggcaaaac catcaccttc gactccggcg gcatttcgct caagcccggc
    10801 aaagggatgg aatggatgcg ctacgacaaa tccggcggca tggccgtact ggccgcgatg
    10861 cagatggtcg cccggctcaa accggaaacg ccagtcatcg gcatgctggc cgtggccgaa
    10921 aatatgcccg gcggccgcgc cacccgcccc ggagatatcg tcaaatcgca cgccggaaaa
    10981 accattgaga ttttaaacac cgacgccgaa ggccggctcg tcctcgccga cgcgctttcg
    11041 ctcgccctgc agcacaaacc cgccgccatc gttgacctcg ccacgctcac cggtgccgtc
    11101 gtcaccgcgc tcggccataa tgccgccgcg gtcgtcggca acaaccacaa gctgaacgcg
    11161 acgctgatca aaaccgccga cgccgctggc gaacggttat gggaactgcc gctctggccg
    11221 gaaaatatgg acgacatgaa aggcaccttc gccgacctgc agaacatgag caaatccgga
    11281 accgccggaa ccagcaacgg cgcggccttc ctcgccaact ttgtccccga aaccattccg
    11341 tgggcgcacc tcgacatcgc cggaaccgcc tgggaagaat cttccaaacc ctggatggat
    11401 cccggcgtca ccctcttcgg
//
LOCUS       DAOPZO010000054        11228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_6626_length_11228_cov_8.245413, whole genome shotgun sequence.
ACCESSION   DAOPZO010000054 DAOPZO010000000
VERSION     DAOPZO010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 11228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11228
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6626_length_11228_cov_8.245413"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..119
                     /locus_tag="PKI68_04935"
                     /pseudo
     CDS             48..119
                     /locus_tag="PKI68_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            327..683
                     /locus_tag="PKI68_04940"
     CDS             327..683
                     /locus_tag="PKI68_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53071.1"
                     /translation="MNSVTNSKTFGINYTGGTNSLPVQFIATNSVESAYSAALSAGIA
                     GVDYFAASVSQSADGSRTITWPAANDPLTRTYKVWYTLDLQQDFQWLATVENGTTYMD
                     TAHADAKVIYYKVTVQ"
     gene            828..900
                     /locus_tag="PKI68_04945"
     tRNA            828..900
                     /locus_tag="PKI68_04945"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:861..863,aa:Lys,seq:ttt)
     gene            complement(923..1360)
                     /locus_tag="PKI68_04950"
     CDS             complement(923..1360)
                     /locus_tag="PKI68_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53072.1"
                     /translation="MKLFIKPHNETAREFYRNHGHFHDGDAGLDLYVLEDITFAPGET
                     KPIKLGISCAPEDGRAYYLFPRSSISKTPLRMSNSIGLIDGGYRGEIMAMCDHIKDTP
                     YTAEKGQRLFQLVATDSSPIHYELVENLSETTRGAGGFGSTGA"
     gene            1453..2025
                     /gene="purN"
                     /locus_tag="PKI68_04955"
     CDS             1453..2025
                     /gene="purN"
                     /locus_tag="PKI68_04955"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNX53073.1"
                     /translation="MLNLAVFGSGSGSNYQAIAEAIAAGRLNARVACVISDVKEAYIL
                     ERARKFGHPAIYLDCAPFKTKLDGEAEARAIEIIKQHGGDIIALAGFMRIVKSGLLNA
                     FAGRIINIHPSLLPAFPGLEAGRQAIEAGVKETGCTVHFVDGGIDSGPIICQRKVPVL
                     PGDTEETLMNRIHEQEHLAYPEALQKIAEG"
     gene            2165..3037
                     /gene="hisG"
                     /locus_tag="PKI68_04960"
     CDS             2165..3037
                     /gene="hisG"
                     /locus_tag="PKI68_04960"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNX53074.1"
                     /translation="MKKLIIGLPKGSLQDSTYALFAKAGFNISGSSRSYFPSIDDKEI
                     ELRLLRPQEMSRYVENGMLDAGIAGLDWIEANGSDVQDVCNLVYSKQTKRPVRWVLAV
                     PENSTIQSVKDLEGKRIATEGVGIVERWLAKNGVKATVEFSWGATEVKVPEFADAIVD
                     ITETGSSLRAHNLRIVDTLMESYTKFFANKTAWADSWKRSKLEKIALLLSAALNATDK
                     VLLKMNVEEKNINRVKKLLPALHSPTVSSLSDTGWYAVETVVNESVVREIIPELKAAG
                     AEGIIEIGLNKVVA"
     gene            3170..5194
                     /locus_tag="PKI68_04965"
     CDS             3170..5194
                     /locus_tag="PKI68_04965"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX53075.1"
                     /translation="MCASRHIRVSGAVVLGVLLGLSGCVSPSKPQLSVNTKAEALSHF
                     SLGLLAETGGDSAAALDHLKAAIRLDPDEEKLYAPAMAIALKLDKPDDAVGLAQALVK
                     RRPGTAEPLLLLARVYAVTKHPEQAETLLKQALSGFPEHPETPVLLAKLYISQNRRTE
                     AMETLRTAAATQSRNAELFHLLGTLCIDNARTQGNTLQARAAVEEGIVFLQKALDIEP
                     EDPVRWQHLGFALLEIQKPEAALGAFRTAHEKNPGDLIAARQVMELLIATGKIDEAMA
                     SYEQIAEETGTEPDAWLQYIAEKLPKDAQDRLITHLEKQIQEEPKSPVFFYAQLGALY
                     IGAHKNQEAEAVLLKARGYYPEDNRLRIVLGYLHLQQERYTEAYTDLEYVRTKAPAAE
                     WSANPFFLINFLVSAQKSGHLEEAAKTLADTYTENPVVLNQYMSSLLTGQTPVSTENA
                     IELLNVFHTLSPEAAEALYYLMVLQAEQKEYQKAIETAKQFEALVQKSGNTNLLGGQF
                     YYQYASLCERSGQFESAEKLFFKVIDLGGDAVPAAQNYLAYMWADRGEKLDQGLALIR
                     KALEADPENGAFLDTLGWIYYRQGRYADALKELQKALCVVNNDADIWTHLGDTYLKLG
                     NRNAAIEHWEKALEIDPKSQELRDRLNAAGIRTGAGPAPADSPADTTIRP"
     gene            complement(5133..6056)
                     /gene="fmt"
                     /locus_tag="PKI68_04970"
     CDS             complement(5133..6056)
                     /gene="fmt"
                     /locus_tag="PKI68_04970"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNX53076.1"
                     /translation="MRVIFFGSATIGFPLLEALLASPKDKVAAVVTQPDRPAGRKQQL
                     TPCPIKLLAQERGLPVLSPEKIKDGLPELAALNADLFVVVAYGQYIPPSVLALPKHGA
                     INLHPSLLPKYRGSSPIQWAVAGGDTVTGVTILYVSEKMDAGDIILQRNVPIGPDDTS
                     ATLEPVLANAGAELLMEAVEQIRSGTVQRHPQDETAATEVRKLTKEDGRIDWNLSAEI
                     LRNRIRGFTPWPGCFCEMPDGQRLKVVRVAVEARKGAAGEILETTGAGPLVAAGEGAL
                     RLLAVQPSGKCVMDGSSYLRGYPLAPGLRLS"
     gene            complement(6073..7875)
                     /locus_tag="PKI68_04975"
     CDS             complement(6073..7875)
                     /locus_tag="PKI68_04975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNX53077.1"
                     /translation="MAKQHQQPFESTLQDLVYSVDAGLGLKIIRTTLFCLMVIILMIL
                     FTARQFRGFDTEAAMDYAQLGRNLAQSNRYITQCVRPASVATVATHSFDGDARIDRHP
                     ELIKPPLYPAILAANFKFFNLVGIDLFPTSTAFQGIRIYPAEQWVIVPLQHFFTMLSG
                     LMLYLLGRKLFSHKIGLLSVLTYFLSEIVWMDSIRGTGLPVLMFFVLGATYFALVAMI
                     NRRERKPLWNWMLFFCLSIGFSAAAFLTHYAALAVIPGLALFIWTMGSRTQRGGHLAF
                     FYVALIFLIVFPWLLRNFQLSGSPLGLASHTALIDSAKYPGDSMMRTLKPDFNLMADI
                     QVVKAKWAKNFNGLYENGFTSLGGGLLIAFFMVTFFYRFVRIHVHNLRWGIGLSMILF
                     FIAACCFDEETMRLYHIFWPFVILYGLAFFSILLDRLDISIQLYKMGLTALVILLTAL
                     PMIVTIFLSSAPGLPYPPYYAPFVMRVSELLKPTEVMCTDMPWATAWYGKRISILLPQ
                     TLDDYYEINDYRKYISGLYITTLTKDRPLVSSLLEGSEKTWFPITMGRLPPNFPLKQG
                     FALNKQDQLFLTDTVRWGADQVQKPESGAQQQKK"
     gene            complement(7946..9298)
                     /locus_tag="PKI68_04980"
     CDS             complement(7946..9298)
                     /locus_tag="PKI68_04980"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNX53078.1"
                     /translation="MKPVILIVDDEKNTREGLARALRRSYDVLIAESGAAALSILSEK
                     PVDVMLSDLRMPGMDGMTLMQRALAVSPQLVCILLTAYGNIETAVDAMRHGATDFLTK
                     PVNLEQLEMVLQRVLRSRRAETENRQLREQLDSKFGMENIIGSSPEMQQVFDTVRQVA
                     ASRATVLIQGESGTGKELIAKAIHRLSPRKNGAFIPVHCAALSSSLLESELFGHEKGA
                     FTGAAERRKGRFEMADGGSLFLDEIGEIDASVQVKILRALEERRFERVGGQESIDVDA
                     RLIAATNRDLKKMVADGTFREDLYYRLYVVVIHLPALRERKSDIPLLLKHYLDVFNQE
                     NGRAVEGFSPDALDLLTAYRWPGNVRELRNLVEQTVVMSRSQRIGVRDLPLHIRETGG
                     TGGPVQMERGTLEDMEKQAIQQALREAGGNRTRAAEKLGISRRTLHRKIAEYGFTEVE
                     "
     gene            complement(9334..9795)
                     /locus_tag="PKI68_04985"
     CDS             complement(9334..9795)
                     /locus_tag="PKI68_04985"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNX53079.1"
                     /translation="MNYDEWEKTVPADLSDDALWKMKVYRYALFLSDIAWLDASKVVQ
                     DVRIKSLSDQLYRAVGSVSANLEEGYSKLSAKDRARFYEYALGSARESRGWYYRIRHV
                     LGEEVFTHRAAFLTEIIKMLLKIVPEERGMRIEEDSVEYKVKNLLDKPVLF"
     gene            complement(9834..11009)
                     /locus_tag="PKI68_04990"
     CDS             complement(9834..11009)
                     /locus_tag="PKI68_04990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX53080.1"
                     /translation="MKSKFLDKLIDRLERMDADSVQAQFLHLAREKGLMETIFQAIQE
                     GIVVVGRGARIRYANRKAEALFGFKLADAENQPIARYIRDIDWEKVLSLDGDEWERLV
                     RREIEVTYPDHRFVEFYMVPLSAIEEREQGAVVIFRDVTRERETTAESIESERLKALT
                     LLAAGVAHEIGNPLNAVTIHLQLMERELADLADYEARESLKELVEVSKREVHRLDRII
                     TQFLRAIRPSLPDRKPVQMDRLLDETLELLRHEVENRRILVERKQAGHIPSVPADEVQ
                     VKQAFFNVIKNAIQAMADGGILKINIDVSPRFVSICFADNGPGIAPENLGAIYEAYHT
                     TKATGSGLGLMIVQRILRDHGGEIEICSTPERGTAFTLHFPRDDVRLSLLEAPKRNE"
BASE COUNT         2721 a         3272 c         2876 g         2359 t
ORIGIN      
        1 actgaatccg gcttttgtgg ttttgtgaaa ccggggtttt tcaggatcga ctaaactaca
       61 ataccatcag gccgcacagc tcactgggat accgacctcc cgctccagag gctcaacaac
      121 ctagaccttc caatatcgcc cgatggcggg tggcgcgaag agttgacttc gctcaaatcc
      181 taacttagaa actggatcca cacacggggg caggtcacag cccgatcggt atcataacgg
      241 caagtccgct ttccggaacc attccggtcg gtgaaacccg taccgtcacc ctgttcggtg
      301 atgcacgcag cctgacatcc aacggggtga attcggtcac gaacagcaaa acgtttggta
      361 tcaactacac aggcggaaca aattctctgc ctgttcagtt tattgcgacg aacagcgtag
      421 aaagcgctta ttctgcggca ctgtccgccg gaatcgccgg tgtggactat ttcgcggcaa
      481 gtgtctcgca gagtgccgac ggatcgcgga caatcacctg gccagccgcc aacgaccccc
      541 tcacccgcac ctataaagtg tggtacacgc tggatctgca gcaagacttc caatggctgg
      601 ccaccgtcga aaacggaacc acttatatgg ataccgcaca tgccgacgcg aaggtgatct
      661 attacaaagt cacggtgcaa taaccgtaag tctgcatcct caacaaaaag cccttccggt
      721 gacggaaggg ctttttttat tcatgctgat atttgatccg actttttttt gtttggcttt
      781 tgacaaccgc tccgcactca tctactttat gcgctccgtt tttttaaggg ccggtagctc
      841 agttggtaga gcatttgact tttaatcaaa gggtcccggg ttcgagcccc ggccggctca
      901 ctcttttaca aacggaatct gtttaagcgc ctgtactccc gaacccgccc gcaccgcgcg
      961 tggtttctga aagattctca accagctcgt agtgaatcgg ggaactgtct gtcgccacca
     1021 gctgaaagag ccgctgcccc ttctcggccg tatacggcgt atctttaatg tgatcacaca
     1081 tcgccatgat ttcaccgcga taaccgccat caatcaaacc gatcgaattg gacattctta
     1141 aaggagtttt agaaatactc gatcgcggaa aaagatagta ggcccgaccg tcttccggtg
     1201 cgcaggaaat gcccagcttg atgggcttgg tctcgcccgg cgcaaatgtg atgtcttcca
     1261 gcacatacag atcaagtccg gcatcgccgt catgaaagtg tccgtgattc cggtaaaact
     1321 ctctggcagt ctcattatgc ggtttaatga aaagcttcat catcttcctt tctgcggatt
     1381 agggattgga cagcagatta aaatccggtt tatccttcga atccagtcaa aacagggaaa
     1441 ggaaccgtac taatgctgaa tctggccgtt tttggatcgg gatcaggctc taactatcag
     1501 gccattgccg aggcaatcgc tgccggacgc ctcaacgccc gggtcgcctg cgttatctct
     1561 gacgtcaagg aagcctacat ccttgaacga gcccgcaaat tcggccatcc ggccatttat
     1621 ctggactgcg ctccgttcaa aaccaaactc gacggcgaag ccgaagcacg cgccattgaa
     1681 atcattaaac agcacggcgg cgatataatc gccctcgccg gcttcatgcg gatcgtcaaa
     1741 tccggcctgc tcaacgcctt tgccggacgt atcatcaaca tccacccgtc cctgctcccg
     1801 gcatttccag gtctcgaagc cgggcgtcag gcgatcgagg ccggagtcaa agaaaccggc
     1861 tgcactgtcc acttcgtcga cggcggtata gatagcggac cgatcatctg ccagcgcaag
     1921 gtgccggtgc tccccggcga taccgaggaa accctgatga accgcattca tgaacaggaa
     1981 caccttgcct atcccgaagc ccttcaaaaa atcgccgaag gataatctgt ttttcagtaa
     2041 aaagccgccg ccggacctgc cggttttcaa tgtttggaaa aagggtaaat aacggtagac
     2101 gactccggcg gttttccgta tagtccgacg ctcttattca agaaatggac ggtgttttta
     2161 gagaatgaaa aaactgatta taggattacc gaagggcagt ctgcaggact ctacgtatgc
     2221 tctttttgca aaagccggat tcaacatctc gggcagctcg cgttcctatt tcccttctat
     2281 tgacgacaaa gagattgaac tccgcctgct ccgcccgcag gaaatgagcc gctacgtcga
     2341 aaacggcatg ctggatgccg ggattgccgg gctggactgg attgaagcca acggctccga
     2401 tgtgcaggat gtgtgcaacc ttgtttactc caagcagacc aaacgccccg tgcgctgggt
     2461 gctggccgtt ccggaaaatt caacaatcca gtccgtcaaa gacctcgaag gtaaacggat
     2521 cgccaccgaa ggcgtcggca tcgtcgaacg ctggctcgcc aaaaacggcg tgaaagccac
     2581 tgtggagttc tcatggggcg caactgaagt caaagtgcct gaatttgcgg acgccattgt
     2641 agacattacc gagaccggct cctcgctgcg tgcgcacaac ctgcgtatcg tggacacgct
     2701 gatggagtcc tacaccaaat ttttcgccaa caaaacggcc tgggccgata gttggaagcg
     2761 ttcaaaactt gagaaaattg cactgctgct gtcggctgct ttaaacgcta ctgacaaggt
     2821 gctgctcaaa atgaatgtgg aagaaaagaa tattaaccgg gttaagaaac tgcttccggc
     2881 tctgcactcg ccgaccgtaa gcagcctgtc tgacaccggc tggtatgccg ttgaaaccgt
     2941 agtgaatgaa tcggtggtga gggaaattat tcctgaactc aaagcggccg gtgccgaagg
     3001 aattatcgaa atcggcttaa acaaagtggt tgcataagcg cacaaaggaa acagatacat
     3061 gggtaccatt aaaaaatggc gtaagaccaa gatgtcgaaa cataaacggc gcaaacgtcg
     3121 tcgtgaagtt cgccacaaaa agaaatagtt ttttataaag gggagtgtca tgtgcgcatc
     3181 ccgccatatc cgggtttccg gagcggttgt gctcggtgtc ctgctgggcc tcagcggatg
     3241 cgtatctcct tcaaagcctc agctgagtgt aaatacgaag gctgaggctt tgtctcattt
     3301 cagtctcggt ctgctggccg aaaccggagg agattcggcc gcagcgctcg atcacttgaa
     3361 ggctgccatc cggctggatc cggatgagga gaagctctac gccccggcca tggcgatcgc
     3421 gcttaaactg gacaagccgg acgatgcggt cgggctggcg caagcgcttg taaaacgccg
     3481 gcccggcacc gctgagccgc tgctcctgct tgccagagtc tatgccgtaa cgaaacatcc
     3541 ggagcaggcc gagacgttgc ttaagcaggc cctttccgga ttcccggaac atccggagac
     3601 tccggttctt ctcgccaaac tttacatttc gcaaaaccgg cggaccgagg cgatggaaac
     3661 actccgcact gcggccgcaa cccagagccg gaatgcagag ctgttccatc tgctgggaac
     3721 gctttgcatc gataacgccc gtacgcaggg caatacgctg caggccagag ccgctgttga
     3781 agaaggaatt gtcttcctgc agaaggctct ggatatcgaa ccggaagacc ctgtgcgctg
     3841 gcagcatctc ggttttgcat tgctggaaat ccagaaaccg gaagcggctc tgggcgcctt
     3901 caggactgca catgaaaaga acccgggcga cctgatcgca gcccggcagg tcatggaact
     3961 gctgattgca accggcaaaa ttgatgaagc catggcgtcc tatgaacaga tagccgaaga
     4021 aaccggaaca gaaccggatg cctggctgca atatattgcg gaaaaacttc cgaaagatgc
     4081 acaggaccgg ctgatcactc atctggaaaa acagatacag gaagagccga aaagcccggt
     4141 tttcttttac gcacagctcg gcgccctcta catcggcgca cataaaaatc aggaagccga
     4201 agccgtcctg ctgaaggcgc gcggatacta tccggaggac aaccgcctgc gcattgtact
     4261 gggctatctg catctgcagc aggaacgcta cactgaagcc tataccgatc ttgagtatgt
     4321 gcggacgaaa gccccggcgg ccgaatggtc ggcaaacccg tttttcctca tcaactttct
     4381 ggtctccgcg cagaaaagcg gccatctgga agaagccgcc aaaaccctgg ccgacaccta
     4441 caccgaaaat ccggtcgttt taaaccagta catgagctcg ctgctcaccg gccagacgcc
     4501 ggtttcgacc gaaaacgcca ttgagctgtt gaacgtcttc cacacactga gcccggaagc
     4561 cgcagaggcg ctctattacc tgatggtgct gcaggcggag cagaaggaat atcagaaagc
     4621 gattgagacc gcgaagcagt ttgaagcact cgtccagaaa agcggaaaca caaacctcct
     4681 cggcggccaa ttttattatc agtacgcttc gctctgtgag cgcagcggac agttcgaatc
     4741 cgccgaaaaa ctttttttca aagtgattga tctgggcgga gacgccgtcc cggcggcgca
     4801 gaattatctg gcttacatgt gggccgaccg cggcgaaaaa ctggatcagg ggctggcgct
     4861 gatccggaaa gctctggagg cggatccgga aaacggggct tttctcgata ccctcggctg
     4921 gatttattac agacaaggcc gctatgcgga tgctttgaag gaactgcaga aagccctctg
     4981 cgtcgttaac aacgacgcgg atatatggac gcatctgggc gacacctatc tgaaactcgg
     5041 caatcgcaac gccgccatcg agcattggga aaaagcgctt gagatcgatc cgaaatctca
     5101 ggaactgagg gatcggctga atgcagccgg catcaggaca ggcgcaggcc cggcgccagc
     5161 ggatagcccc gcagatacga cgatccgtcc ataacgcatt tgccggacgg ctgcacggcg
     5221 agcagccgca aagccccctc gcctgccgca accagcggac cggctccggt tgtctccaga
     5281 atttcgccgg ccgctccttt acgcgcctca acggcgaccc tcacaacttt caggcgctga
     5341 ccgtccggca tttcgcagaa acagccgggc cagggagtaa acccgcgaat ccggttgcgg
     5401 agaatttctg cggaaagatt ccagtcgatc cgcccatctt ctttagtgag cttgcgcact
     5461 tccgtcgcgg cggtctcgtc ctgcggatgg cgctgaaccg ttccgctccg gatctgttca
     5521 accgcttcca tcagcagttc cgcgcccgca ttcgccagta ccggttccag cgtggctgat
     5581 gtgtcgtccg ggccgatcgg cacgttgcgc tgcagaataa tgtcgcccgc atccattttc
     5641 tcgctcacat aaagaatcgt gacgccggtg acagtgtcac cgccggcgac cgcccactga
     5701 atgggtgatg aacctcggta tttcggaagg agtgaaggat gcagattgat cgcaccgtgt
     5761 ttcggaagcg ccagaacgga cggcggaata tactggccat aggcgacgac aacaaagagg
     5821 tcggcgttta aagcagccag ttccggcaga ccgtccttga ttttttccgg agaaagaacc
     5881 ggcaggccgc gctcctgtgc gagtaattta atcgggcacg gtgtgagctg ctgtttgcgt
     5941 ccggccggcc ggtccggctg ggtcaccacg gcggcgactt tatccttcgg acttgcgagc
     6001 agcgcttcga gcaatggaaa tccgatggtt gctgatccaa aaaaaataac acgcatgatg
     6061 taacagcccc ggttacttct tctgctgctg cgcgccggat tcgggtttct gcacctggtc
     6121 agcaccccag cgcacggtat cggtcaaaaa cagctgatcc tgtttattga gcgcaaaccc
     6181 ctgctttaaa ggaaagttcg gcggcagccg gcccatcgtt atcggaaacc aggttttctc
     6241 agatccttcg agcagggagg aaacaagcgg acggtctttg gtgagggtgg tgatgtaaag
     6301 tccgctgata tatttacggt agtcgttaat ttcgtaatag tcatcgagcg tttgcggcag
     6361 aagtatggag atccgctttc cataccacgc agttgcccac ggcatgtccg tacacattac
     6421 ttcagtcggc ttaagcagtt cgctcacgcg cataacaaac ggagcgtaat acggcggata
     6481 tggcagtccc ggcgcagacg acagaaagat ggttacaatc atcggcagtg cggtgagcag
     6541 aatcaccagc gccgtcagtc ccattttgta gagctgaatg ctgatgtcca gccggtcgag
     6601 caggatagag aagaaagcca gaccgtagag aatcacaaac ggccagaaaa tatgatacag
     6661 ccgcatggtt tcttcatcaa aacagcaggc agcgatgaaa aacaaaatca tcgaaaggcc
     6721 gattccccag cgcaggttgt gaacgtggat tcgcacgaaa cggtaaaaga aagtcaccat
     6781 gaaaaaggcg atcagcagtc caccgcccaa cgaggtgaat ccgttttcgt agagtccgtt
     6841 gaaatttttc gcccatttgg ctttcaccac ctggatatcg gccatgagat taaaatccgg
     6901 tttcagcgta cgcatcattg agtcgcccgg atacttggcg gaatcaatca gcgcggtatg
     6961 agacgcaaga cctaacggcg agccggaaag ctggaagttg cgcaacagcc aggggaatac
     7021 gatcaaaaag atcagcgcca cataaaagaa ggccaggtgg ccgccgcgct gtgtccgaga
     7081 acccatcgtc cagataaaca acgccagccc cggaataacg gcaagcgccg cataatgcgt
     7141 caaaaacgcc gcagcggaaa acccgatcga aagacagaaa aacagcatcc agttccacag
     7201 aggtttgcgc tcccggcggt taatcattgc caccagagcg aaataagtcg cgcccagcac
     7261 aaagaacata agaaccggca gtcctgtccc gcgaatactg tccatccaga cgatttcgct
     7321 gaggaaatac gtcagaacgc tcagcagacc gattttgtgc gaaaacaatt tccgccccag
     7381 caggtaaagc attagaccgg aaagcatggt gaaaaaatgc tgcaggggaa caatcaccca
     7441 ctgttcggcc ggatagatgc ggatgccctg aaatgctgta ctggtcggaa acagatcaat
     7501 gccgaccaga ttaaaaaatt taaaattcgc cgcaagaatc gccggataaa gcggcggttt
     7561 gatgagttcc ggatggcggt caatccgcgc atcgccgtca aaactgtggg ttgctaccgt
     7621 tgcgacactc gccggacgga cacattgcgt aatatagcgg tttgactgcg ccagattccg
     7681 tcccagctgc gcgtaatcca tggccgcctc ggtatcaaat ccgcggaact ggcgggctgt
     7741 aaaaagaatc atcagaataa tgaccatgag acaaaaaaga gtcgtgcgga tgatcttcag
     7801 ccccagacct gcatccacgc tgtacacaag atcctgcagg gtcgattcaa agggttgctg
     7861 atgttgcttt gccatactgt ttctcccgct ccggtaatga gtgttcaaag catccttgaa
     7921 cacttctgct tcttcaacct taaactcatt cgacttcggt aaaaccgtat tccgcgatct
     7981 tgcggtgcag cgtacggcgg ctgatgccca gcttttcagc ggcccgggtc cggttgccgc
     8041 ccgcttctct aagcgcctgc tgaatggcct gtttttccat atcctccagc gtgccgcgtt
     8101 ccatctgaac cggaccgcca gttccgcccg tctcacggat atgcagcggc agatcacgca
     8161 caccgatccg ctgcgaccgg gacatgacca ccgtctgctc caccagattg cgcaattcgc
     8221 gcacattgcc cggccagcga taggccgtca gcagatcgag tgcatccgga gaaaaaccct
     8281 ccaccgcgcg gccgttttcc tgattaaaca catccagata atgtttcagc agcagcggaa
     8341 tatcgctttt acgctcacgc agcgccggca gatgaatcac caccacataa agccggtaat
     8401 acaaatcctc gcgaaacgtg ccgtccgcca ccattttctt aagatcccgg ttcgtcgccg
     8461 caatcagacg tgcatcgaca tcaatggatt cctgaccgcc gacacgctca aagcgccgtt
     8521 cctccagcgc gcgcaggatt ttaacctgca cactggcatc gatctcacca atctcatcca
     8581 gaaacaacga gccgccgtcc gccatctcaa aacgtccttt gcgccgttcc gccgcgccgg
     8641 taaacgcccc cttctcatgg ccgaacagtt cgctctccag cagcgacgaa gaaagcgcgg
     8701 cgcagtgcac cgggataaaa gccccgttct tacgcggact cagccggtga atcgccttgg
     8761 cgatcagctc tttgccggtt ccgctctcgc cctgaatcag caccgtcgcc cgcgacgccg
     8821 caacctgccg caccgtatca aagacctgct gcatctccgg cgagctgccg atgatattct
     8881 ccatcccgaa cttactgtcc agctgctcgc gaagctgacg gttttccgtt tcggcccggc
     8941 gcgaacgcag cacgcgctga agcaccattt ccagctgctc cagattcacc ggcttggtca
     9001 gaaaatcggt cgcgccgtgt cgcatcgcat caaccgccgt ttcaatattt ccgtaggcgg
     9061 tcagcagaat acaaaccagt tgcggtgaca ccgccagcgc gcgctgcatc agcgtcatcc
     9121 cgtccatacc gggcatccgc agatcgctca gcatcacgtc gaccggcttc tcactcagaa
     9181 tactcagcgc cgccgcgccg ctttcagcaa tcagaacgtc gtacgaacgg cgcaacgccc
     9241 gcgccagccc ttcgcgtgtg ttcttttcgt cgtccacgat taaaattacc ggtttcatgt
     9301 tcgtactccg tattacgtac tgcgtattat tttctaaaat agaacgggtt tatccaaaag
     9361 gtttttgact ttgtattcga ccgaatcctc ctcgatgcgc atccctcgtt cctccggaac
     9421 gatcttcagg agcattttga tgatttcggt tagaaacgct gcacgatgag taaaaacctc
     9481 ttctcccagc acatgtctga tccggtaata ccagccacgg ctctcacgcg ctgatcccaa
     9541 tgcgtattca tagaaccggg cccggtcttt cgcgctcagc ttggaatagc cttcttccaa
     9601 attggcagac acagatccaa cagctctata aagctgatcc gaaagagatt ttatccgcac
     9661 atcctgcacc accttcgacg catcaagcca ggcaatgtca gacagaaaaa gagcatagcg
     9721 gtaaactttc atcttccata gcgcatcatc actcaaatct gcaggaaccg tcttttccca
     9781 ctcatcataa ttcataagtt acctcttcca aaatacgcag tacgcaatac tccctactcg
     9841 ttacgctttg gcgcttcgag aagcgacaag cgcacatcgt cccgcgggaa atgcagcgtg
     9901 aaagcggtgc ctcgttccgg cgtactgcaa atttcaattt caccgccgtg atcgcgcaga
     9961 atccgctgca caatcatcag gccgagtccg gaacccgtcg ccttggtcgt gtgatacgcc
    10021 tcataaatcg cgccgagatt ctccggtgca attcccggac cgttatccgc aaagcaaatg
    10081 ctcacgaaac gcggcgacac gtcgatattg atcttcagaa tgccgccgtc cgccatcgcc
    10141 tgaatcgcgt tcttaatgac attaaagaac gcctgcttca cctgaacctc atcggccgga
    10201 accgacggaa tatgcccggc ctgtttgcgc tcgaccagaa tacggcggtt ttcgacttcg
    10261 tggcgcagca gctccagcgt ttcatccagc agccggtcca tctgcaccgg cttgcggtcg
    10321 ggaagcgacg gccggatcgc gcgcagaaac tgcgtaatga tccggtccag gcggtgaacc
    10381 tcgcgcttcg aaacctcgac cagctctttc aggctttcgc gcgcttcata atcggccaga
    10441 tcggccagtt cgcgctccat cagctgtaag tggatcgtca ccgcattcag cggattaccg
    10501 atttcatgcg ccacgcccgc cgccagcagc gtcagcgcct tcagccgttc ggactcgatg
    10561 gactcggcag tcgtttcacg ctcgcgcgtc acgtcgcgga aaatcaccac cgcgccctgc
    10621 tcgcgctctt cgatcgccga aagcggcacc atataaaatt ccacaaaccg gtgatccgga
    10681 taggttacct cgatttcgcg gcgaaccaga cgctcccact cgtcgccgtc cagactgagc
    10741 accttttccc agtcgatatc gcgaatatag cgggcaatcg gctggttttc ggcgtctgcg
    10801 agcttaaagc cgaaaagcgc ttccgccttg cggttggcgt accggatgcg ggcgccgcgc
    10861 ccgaccacca cgataccttc ctgaatcgcc tgaaagatcg tctccatcag ccctttttcg
    10921 cgggccagat gcaaaaactg cgcctgaacg ctgtcggcgt ccatccgctc aagacgatcg
    10981 atcaatttat ctaaaaactt cgatttcaca gggaaatcct ccgcttaaac cgggacaatt
    11041 tgtaccacag ctccggcgga gttcaaccgc ttaatagcgc cagaatggca aatgccgata
    11101 aattttgtag ccgaggtccg cgacctcggt ttagaccgcg atcacggatc gcggctacaa
    11161 aaccatattc tcgcctcctg aacgaggaca tcaaacttga ccgcctacca tcttcccgca
    11221 atagttcg
//
LOCUS       DAOPZO010000055        11166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_6689_length_11166_cov_9.191342, whole genome shotgun sequence.
ACCESSION   DAOPZO010000055 DAOPZO010000000
VERSION     DAOPZO010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 11166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11166
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6689_length_11166_cov_9.191342"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PKI68_04995"
     CDS             complement(<1..518)
                     /locus_tag="PKI68_04995"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNX53081.1"
                     /translation="MRGSTGIEGNRCSVEQVEAIVRGETVALSQKEQLEIRNALEAYA
                     ALPEFDPFSVDSFLNAHKKLMGNGLELAPGIFRRGSVEVYITETETRAMPAWETVEPS
                     MRALFDYLRTSEELMLIKSIRFHFECVNIHPFFDGNGRTARLWQTRLLMLEHTVFEFL
                     DVESMVFEQREE"
     gene            complement(721..1404)
                     /locus_tag="PKI68_05000"
     CDS             complement(721..1404)
                     /locus_tag="PKI68_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53082.1"
                     /translation="MTGRMLRKRSQLFFLWFQVLFLASVIGGYTNWFRERMDLVATLL
                     MIGFCVLALVLLLPVHVFCAIRSSMARVGWLDIVLLVVSTALFVLIVSLTNIKLSGHL
                     YPPLEGRVFKDESRVFVDDEGRNIEWYVEFANPYLASHKERLVLIDGKQKKIIKTKLL
                     SDEKVFGRTGSDRISLRQIGGNILLTSTIEISPTTRFVLIDYKSRTVISNWLEQALWA
                     KPTQVEPAP"
     gene            complement(1443..2750)
                     /gene="hisD"
                     /locus_tag="PKI68_05005"
     CDS             complement(1443..2750)
                     /gene="hisD"
                     /locus_tag="PKI68_05005"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNX53083.1"
                     /translation="MKKTDAPILKYSAKGRSTRIEDFLLRPPVEKGADKIAAGVLADI
                     KKDGDSAVVKYARQFDRSTVTLETICVSQTELAEAKKAVDAGFKKAAKEAHKRITEFA
                     VAGLREDWRMPAPQGGWLGEKFVALDRVGAYIPGGAAPLASTALMTLTLAQVAGVPER
                     VACTPANAEGKVNPYILYALNLAGATEIYKVGGIQAIGAMAYGTKTIKKVQKIVGPGG
                     PYVTAAKRLVYGEVALDMVAGPSEIAVLADETANPAHVAADLLSQAEHGTGSEKALLV
                     TASEKLAKAVRSELEVQSAKLNRQAPVAQVLAHGCLLVVVENIEKGIELCNRFAPEHM
                     EVIVKNAERVAAKIYRAGAIFIGPWTPEAVGDFAAGPSHVLPTGGTAAFFSGLTVEDF
                     RRRVSLIHFTKEDLKETVSVVEAFGRVETLDAHARSATIRLGK"
     gene            complement(2758..3708)
                     /locus_tag="PKI68_05010"
     CDS             complement(2758..3708)
                     /locus_tag="PKI68_05010"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX53084.1"
                     /translation="MKKYHFNKRVLVTGGAGFLGSHLCEALLKRDCDVVCADNFFTGR
                     RGNVAHLLDNPRFEILRHDVTFPLFVEVDEIYNLACPASPPHYQHDPVQTTKTSVIGA
                     INMLGLAKRVGAKIFQASTSEVYGDPEIHPQPESYWGRVNPIGTRSCYDEGKRCAETL
                     FFDYHRQNRVKIKVARIFNTYGPNMHPNDGRVVSNFIMQALRGEPITIYGDGKQTRSF
                     CYVDDLIAGFLALMDSPDDVTGPVNLGNPGEFTMLELAEAVIRLTGSKTELVFKPLPS
                     DDPKQRQPNITVAKEKLGWEPKIPLEEGLKHTIKYFKQYA"
     gene            3762..4322
                     /locus_tag="PKI68_05015"
     CDS             3762..4322
                     /locus_tag="PKI68_05015"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNX53085.1"
                     /translation="METKQKLILASTSPRRVELLKSAGFAFEVLRPDADEKLHAGEAP
                     VAYAVRTAREKAESIAARPGTVVLGADTVVAVEGRILGKPADAHDAERMLRLLSGKLH
                     EVITGVCLRSPEKTVCFHAATAVLFRDLSDEEIAAYVATGDPLDKAGAYAIQNGAAGM
                     VRRIDGSYSNVVGLPLCEVIEALENF"
     gene            4322..4999
                     /gene="rsmI"
                     /locus_tag="PKI68_05020"
     CDS             4322..4999
                     /gene="rsmI"
                     /locus_tag="PKI68_05020"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNX53086.1"
                     /translation="MDAGLYIVGTPLGNLGDMTARGIETLKAASLIIAEDTRTTRNLL
                     NHFEIKTHCISCHKFNEAQRCDEIIARIRNGEAVAMVTDSGMPCISDPGARVVEACRA
                     AGVMITSAPGPTAVTTALALSGFGGHGFLFAGFLPRKSGARRRTLEQCAALPVPAVFY
                     ESPYRLLKLLEEIEAVMGADRQVFAARELTKKFEELIRGTPAEIRQRFGERTVKGECV
                     VIIGPGE"
     gene            5078..5596
                     /locus_tag="PKI68_05025"
     CDS             5078..5596
                     /locus_tag="PKI68_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNX53087.1"
                     /translation="MLGVEQSNDDLQAAIAGDKVFIRVTGRGSFKVSTTLKQFIAQVV
                     AQQPISTVVLDLTECIGMDSTFMGVLAGLSGRLKQNSQTLELINLSEKNANLLATLGV
                     DQVIAHYSHSHGHEVPDQNAKSLSIAQVTKKELAETALQAHETLVELSEENRPRFKRV
                     IEYLKADVDRLN"
     gene            5605..7059
                     /locus_tag="PKI68_05030"
     CDS             5605..7059
                     /locus_tag="PKI68_05030"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNX53088.1"
                     /translation="MAEQLSIWITPFLIFILGALTAAVFYRTLGRSRQIRRQNSALRQ
                     EREVIFDFIHNIGEVFADAEEIKADSLLKRILFFATKTGKAASGAIYLYNAERTQLFA
                     RAVSGIFPPLYDAEQVHTEQLLAKSQYLETLVKTRAVAAGEGLIGSSASVGSSIIIED
                     AEIDARVPSYADDFLKIRSLLIVPMRFGNEVLGVIALANRTDGQPFTPGDLNLLQAMA
                     DQASVPVHYTGLKADLDRKRQLDRDMQAAQQIQMSLLPQHLPQLRDVRLAAYNLPAFN
                     IGGDYYDVIQIDNEHLGIAIADVSGKGIGGAMMMAVCQGVLRARGPQEQSPARMLSEL
                     NRVLSANLAEDMFITMLYMVLNTRTRELRLARAGHERPLLKRVEHDKSEALDAAGIAI
                     GLAGADIFDSAIKDVSVQLSPGDVVVLYTDGITEALNEKNEEWGTASLLKTIDSEAAA
                     GVDSLIQAVRSRLVRHVGAQQQYDDMTLIALEVH"
     gene            7157..7705
                     /locus_tag="PKI68_05035"
     CDS             7157..7705
                     /locus_tag="PKI68_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNX53089.1"
                     /translation="MSKKHKTEENKPAQEQPETVIEPAAAEPAVIRKEDEEPLKNQML
                     RLQADFDNFRKRTQRERGELFQMANETLFLEILPVIDHFEMGFKSAEAHKTDCSVTEG
                     FRMVYNQMLDVLKKFNVTVIETVGESFDPHRHESLLHMPSDKPAGTVIEQIRRGYMFG
                     EKLLRAAQVIVSSGPAEQKESE"
     gene            7705..>7937
                     /locus_tag="PKI68_05040"
     CDS             7705..>7937
                     /locus_tag="PKI68_05040"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNX53090.1"
                     /translation="MPGQKRDYYEVLGIAKGASAEEIKKAYRKMAIQYHPDKNPGDKA
                     SEEKFKEVSEAYEVLSDASKRQQYDQFGHAAFGA"
     assembly_gap    7938..8037
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8038..8847
                     /locus_tag="PKI68_05045"
     CDS             <8038..8847
                     /locus_tag="PKI68_05045"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNX53091.1"
                     /translation="PNAPQEGADLRFDLDIDFEEAVLGSSREIRINVNETCERCKGSG
                     AEPGSGRKTCSTCRGSGQVVSGGGFIQFRQTCPACRGAGQVIEKPCTACHGQGLVRNK
                     RTIDVKIPAGVETGSRLRIAGKGEGGLRGGPAGDLYIVLHVREHEFFKRNDLDIVCEI
                     PVPFHIAALGGEVPVPTIHGGAELKIPAGTESGKVFRMKGKGISSPRYGTGDQHVVVQ
                     IEIPQGLGSKEKKKLQEAAEMLNEKHFPLRQKMHKAAEKFYERKRILEKGQ"
     gene            8844..>9396
                     /locus_tag="PKI68_05050"
     CDS             8844..>9396
                     /locus_tag="PKI68_05050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNX53092.1"
                     /translation="MRINSFADPAALRAERVVLAADESHHLARVLRVEAGQELTLFDG
                     EGTRADGVVDAVTKKEVIVRITARETVPPPAVEITLIQAVCKPDRFELILQKATELGV
                     RRIQPVVTQNASLPAGKIEKMMERGVAIIRNAAQQCGTAWMPELLALQKFSALSLKAD
                     AAFIGSLHPNARPFRDAFRALDAS"
     assembly_gap    9397..9496
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9497..10516
                     /locus_tag="PKI68_05055"
     CDS             <9497..10516
                     /locus_tag="PKI68_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNX53093.1"
                     /translation="AWSMFNKNIPLEEYKKTAHEVTASGFNAEEIVKLAEESGMHYIT
                     FVAKHHDGFCLWDSQYTDFDSKDYFNRDLLAELAKACHEHGMPLCIYYSIGIDWTHPY
                     FLPRELYSVGRPKYDAAGNAYFKYRVPEDFENYRTFCKQQLAELSTKYGPVAGFWFDP
                     LGGVLANSEMFKLQEMYDVIRQLQPQALIHFKTGATGTEDILVGERSLAPISSFYKGD
                     SPQNKRIRELSDAAWKANFRKKAEIAVTSQGPWEWSPKVTCRPADGLWKMLLIAADNN
                     ANLLLNFGPKPDGSIPDDVATNFRQLGERIRKEGYPPLNKTTYLEKRQKGVVADDTEK
                     EKTAR"
     gene            10671..>11166
                     /locus_tag="PKI68_05060"
     CDS             10671..>11166
                     /locus_tag="PKI68_05060"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX53094.1"
                     /translation="MSTKVIALANQKGGVGKTTTAVNLAAALAERRKNVLLIDLDPQA
                     NATSALGLEKEEGASIYSALLGEADVRTLIKPTATKRLHMIPSELDLAGCEVAIARMN
                     NYLHCLKNALRPIVEQDEYDVILLDCPPSLGILFMNALYTADSIVIPIQAEYLALEGL
                     SVIVS"
BASE COUNT         2483 a         3216 c         3019 g         2248 t
ORIGIN      
        1 tactcttccc gttgctcaaa caccatggat tccacatcaa gaaactcaaa aacagtatgc
       61 tccagcatca acagtcgcgt ctgccacaaa cgggcggtcc ggccgttgcc gtcaaaaaac
      121 ggatggatgt tcacgcactc aaaatggaag cggatcgact tgatcagcat caactcttcg
      181 cttgttctga gatagtcaaa cagcgcccgc atcgacggct caacggtttc ccatgcaggc
      241 atggcgcgcg tttcggtttc cgtaatataa acctcaacag aaccgcgccg gaaaataccc
      301 ggtgccaatt cgagtccgtt ccccatcagt tttttatgag cattcaaaaa cgaatcgaca
      361 gaaaacggat caaattccgg caaagcggca taggcttcca acgcattccg gatttccagc
      421 tgttccttct gcgaaagagc caccgtttcg ccgcggacaa tcgcttcgac ctgttccact
      481 gaacagcggt tgccttcaat gccggttgac ccgcgcacgg atcgcgcacg gtttttatct
      541 cgaagtttcg gaaccggtcg ggaaaattgt aagccttcca atttgccgat cagctgttgg
      601 atgaccgcat gcttttgcat gcccgctgca gacaacgtga actctgtaag actccaactc
      661 atgtcgctga tagtatcaaa cgatactatc atgtcaatat agtatcatca caccgcttgt
      721 ctatggggct ggctccacct gcgtaggttt tgcccacaag gcctgttcaa gccagttaga
      781 aattactgtt ctagatttat aatcaatcaa tacaaatcga gtcgttggac taatttcgat
      841 tgtactcgtt aacaaaatat tgcctcctat ttgccgaagg gatattctgt ccgaacccgt
      901 acgaccaaat actttttcat cggagagtaa ctttgttttt ataatctttt tttgcttccc
      961 atcaatcaag accagtcgtt ctttatgact agcaagatat gggtttgcga actctacata
     1021 ccactctata ttgcgtcctt catcatcaac aaacacgcga ctttcatcct taaacacccg
     1081 accctctaac ggaggataga gatgaccaga caacttgata ttggtcagag aaacaatcag
     1141 cacaaagagc gccgttgaaa caaccaaaag cacaatatcc aaccaaccga ctctagccat
     1201 cgagcttctt attgcgcaga aaacatgaac tggaagcaat aacacgagcg ccaaaacgca
     1261 aaaaccgatc ataagcaaag tggccactaa atccatgcgt tcacgaaacc agttcgtata
     1321 gcccccgatc actgaggcaa ggaatagcac ctgaaaccat aagaagaaaa gctgacttct
     1381 tttgcgcaac atccgaccgg tcatagtgca cttcgctttt ctttgcgcta attttggcta
     1441 ctctactttc ccagccggat ggtggcggag cgggcgtggg cgtcgagggt ttcgacgcgg
     1501 ccgaacgctt cgaccaccga aacggtttct ttcagatctt ctttagtaaa gtggatcagg
     1561 ctgacgcggc ggcggaagtc ttcgaccgtc aggccggaga aaaaggccgc cgttccgccg
     1621 gtcggcagaa cgtggctcgg cccggcggcg aagtcgccga cggcttccgg agtccacggc
     1681 ccgataaaga tcgcaccggc ccggtaaatt ttcgccgcaa cgcgttcggc gtttttaacg
     1741 atcacttcca tgtgttccgg cgcaaagcgg ttgcaaagct cgatgccctt ttcgatattt
     1801 tccacgacaa ccagcagaca gccgtgcgcc agtacctgtg caaccggagc ctgccggttt
     1861 aatttcgcag actgaacttc aagttcggaa cgaacggctt tcgccagttt ttccgaggca
     1921 gtcaccagca gcgctttttc ggagccggtg ccgtgttcgg cctgcgaaag caggtcggcg
     1981 gcgacgtgcg caggattggc ggtttcgtcg gccagcaccg cgatttcgct cgggccggcg
     2041 accatgtcga gggcgacttc gccgtaaacc agccgcttgg cggcggtcac atacggcccg
     2101 ccggggccga cgattttctg tactttctta atcgtcttgg tgccgtacgc catggcgccg
     2161 atggcctgga tgccgccgac tttataaatt tcggtcgcgc ccgccagatt gagagcatag
     2221 agaatgtacg gattcacttt gccttccgca ttggcaggcg tgcaggcaac ccgttccgga
     2281 acgccggcaa cctgcgccag cgtgagcgtc atcagcgcgg ttgaagcaag cggtgcggca
     2341 ccgccgggaa tgtaggcgcc gacgcggtcg agcgcgacga atttttcgcc gagccagccg
     2401 ccctgcggcg caggcatccg ccagtcttcg cgcagtccgg cgacggcaaa ttcggtgatt
     2461 cgtttatgcg cttctttggc ggctttttta aacccggcat cgaccgcttt tttcgcttcg
     2521 gcaagttcgg tttgggaaac gcaaatcgtt tccagcgtga cggttgagcg gtcaaactgc
     2581 cgggcatatt tcacaacggc gctgtcgccg tcttttttaa tgtccgccaa aacaccggcg
     2641 gcgattttat ccgcgccttt ctcaaccggc gggcggagca gaaaatcttc aatccgggtt
     2701 gagcgccctt tggcgctgta tttcaaaatg ggagcatcgg tctttttcat aatgcggtca
     2761 ggcgtactgt ttgaagtatt tgatggtgtg cttgagccct tcttccagcg gtattttcgg
     2821 ctcccagccc agcttttctt tagcaacggt aatgttcggc tggcgctgtt tcgggtcgtc
     2881 ggacggcagc ggcttgaaga ccagttcggt ttttgaaccg gtaagccgga tgacggcttc
     2941 ggccagttcc agcatggtga actcgccggg attgccgagg ttgaccgggc cggtaacatc
     3001 gtccggggaa tccatcaggg cgaggaatcc cgcgatcagg tcgtccacat agcagaacga
     3061 gcgggtctgt tttccgtcgc cgtaaatggt aatcggctcc ccgcgcaagg cctgcatgat
     3121 gaagttgctg acaacgcggc cgtcgttggg gtgcatgttg gggccgtagg tgttgaagat
     3181 gcgggcaact ttgattttga cgcggttctg ccggtgatag tcaaagaaaa gtgtttcggc
     3241 gcagcgttta ccttcgtcat agcaggagcg cgttccgatg gggttgacgc ggccccagta
     3301 gctttcaggc tgcggatgga tttccgggtc gccgtaaact tcgctggttg acgcctggaa
     3361 aatcttcgcg ccgacacgtt tggccagacc gagcatattg atggcgccga tgacgctggt
     3421 tttggtggtc tgcaccggat cgtgctggta gtgcggcggc gaggccgggc aggccagatt
     3481 gtaaatttcg tccacttcga caaacagcgg aaaggtgaca tcgtgccgca gaatttcaaa
     3541 ccggggatta tccagcaaat gcgctacatt gccgcgccgg ccggtgaaaa agttatccgc
     3601 acaaacgaca tcgcagtcgc gcttcagcag cgcttcgcac aggtgtgagc cgagaaaccc
     3661 ggcaccgccg gtgaccagta cccgtttatt gaagtggtat tttttcatgc tttgcttatc
     3721 ctctgccttc taaaaccggt gggatgctat ctttccaacc tatggaaacc aaacaaaaac
     3781 tgattcttgc cagcacctcg ccgaggcgcg ttgagctgct gaagagcgcg ggcttcgctt
     3841 tcgaagtttt acggccggat gccgacgaaa aactgcacgc cggcgaagcg ccggtggcct
     3901 acgccgtccg caccgcgcgt gaaaaagcgg aaagcatcgc cgcccggccc ggcacggtgg
     3961 tactcggtgc cgacacggtg gtcgcggtgg aaggccggat tctcggcaaa ccggccgacg
     4021 ctcatgacgc cgaacggatg ctgcgtctgc tttccggcaa actgcacgaa gttatcaccg
     4081 gcgtctgcct gcggtcgccg gagaagacag tctgctttca tgcggccacc gccgtactgt
     4141 tccgcgattt gtccgatgaa gaaatcgcgg cctatgtcgc caccggcgac ccgcttgata
     4201 aagccggagc ctatgcgatt caaaacggcg cggcgggcat ggtgcggcgc atcgacggct
     4261 cctattcaaa cgtcgtcggc ctgccgctgt gtgaagtcat tgaagctttg gagaatttct
     4321 aatggatgca ggcctttaca ttgtaggaac accgctcggc aatctgggcg atatgaccgc
     4381 gcgcggcatt gaaaccctta aagcggcttc gctgatcatc gccgaagaca cgcggacaac
     4441 gcgtaatctg ctgaatcact ttgaaatcaa aacccactgc atcagctgcc acaagttcaa
     4501 cgaggcgcag cgctgcgacg aaatcatcgc ccgcatccgc aacggcgaag ccgtggcgat
     4561 ggtcaccgat tccgggatgc cctgcatctc cgaccccggc gcccgcgtgg tggaagcctg
     4621 ccgcgcggcg ggcgtgatga ttacgtcagc gcccggcccg acggccgtga cgaccgcgct
     4681 ggcactgagc ggattcggcg gacacggttt tcttttcgcg ggttttcttc cgcgcaagag
     4741 cggggcgcgg cggcggacgc tggagcagtg cgccgcgctg ccggttccgg cggttttcta
     4801 cgaatcgccc taccggttgc tgaagctgct ggaagaaatt gaggcggtca tgggtgcaga
     4861 ccggcaggtt ttcgcggccc gcgagctgac aaaaaagttt gaagaactga tccgcggtac
     4921 tccggcggaa atccggcagc ggttcggcga gcgcacggtc aaaggcgagt gcgtcgtaat
     4981 aatcggaccg ggcgaataat gttttcgctg attatcgttt gcactgctct atgcagccga
     5041 ctaaactttc gccgcttgaa atgaggaatt ctttattatg ctcggagttg aacaaagtaa
     5101 cgacgatttg caggcggcca ttgccgggga taaagttttc atacgcgtga ccgggcgcgg
     5161 ctccttcaaa gtcagcacga ccctgaaaca gttcattgcc caggttgtcg cgcagcagcc
     5221 gatttcaacc gtggtgctcg atctgaccga atgcatcggc atggacagta cattcatggg
     5281 cgtcctcgcc ggactttccg gacgcctgaa acagaacagc cagacgctgg agctgatcaa
     5341 cctttctgaa aaaaatgcca atctgctggc aaccctcggc gtagatcagg tcatcgccca
     5401 ctacagccac agccacggcc atgaagtgcc ggatcagaat gccaagtcgc tttcgatcgc
     5461 ccaggttacc aaaaaagagc tggccgaaac cgcattgcag gcgcacgaaa ccctggtgga
     5521 actcagcgaa gaaaaccggc cgcgcttcaa gcgggtcatc gaatacctca aagcagacgt
     5581 cgaccggctc aactgaatcc tcttatggct gaacaactgt caatctggat cactccgttc
     5641 ctgattttta tactcggcgc gcttaccgcc gccgtgttct accggacgct cggccgcagc
     5701 cggcagatcc gccggcagaa ttccgcgctc cggcaggaac gcgaagttat tttcgacttc
     5761 attcataaca tcggcgaagt gttcgcggac gccgaagaaa tcaaagccga ttcactgctc
     5821 aaacggattc tgtttttcgc cactaaaacc ggcaaagccg catccggcgc catctacctt
     5881 tataacgccg agcgtacaca gctcttcgcc cgcgccgttt ccgggatttt tccgcctctt
     5941 tacgacgccg aacaggtgca cacggaacag ctgctcgcca aatcacagta tctcgaaacg
     6001 ctggtgaaaa cccgcgccgt tgccgccggc gagggcttga tcggctcttc cgcctccgtc
     6061 ggcagcagca tcatcattga agatgcggaa attgatgccc gcgttccttc ctatgcggat
     6121 gactttctta aaatccgctc tttgctgatc gttcccatgc gtttcggcaa cgaagtgctc
     6181 ggcgtcatcg cgcttgccaa ccgcaccgac ggccagccct tcacgcccgg cgaccttaac
     6241 ctgcttcagg cgatggccga ccaggcttcc gttcctgttc actacaccgg actcaaggcc
     6301 gatctggacc gtaaacggca gctcgaccgc gacatgcagg ccgcgcagca gattcagatg
     6361 tcgctgctcc cgcagcacct gccgcagctc agggatgtcc ggctcgccgc ttataacctt
     6421 cccgccttca acatcggcgg cgactactac gacgtcatcc agatcgacaa tgaacacctc
     6481 ggcatcgcga tcgccgatgt ttccggaaaa ggcatcggcg gcgccatgat gatggccgtc
     6541 tgccagggcg ttctccgcgc ccgcgggccg caggagcaaa gtccggcgcg catgctgtcc
     6601 gaacttaacc gcgtcctgag cgccaatctc gccgaagata tgttcatcac catgctttac
     6661 atggttttaa acacccgcac ccgcgaactc cggctggccc gcgccggcca tgagcgcccg
     6721 ctgctcaaac gtgttgagca tgacaaatcc gaagcgctcg atgccgccgg aatcgccatc
     6781 ggtctggccg gagcggatat tttcgacagc gcaatcaaag atgtttccgt ccagctttcc
     6841 cccggcgacg tcgtcgtcct ctataccgat ggcatcaccg aggcgctcaa tgagaaaaac
     6901 gaagaatggg gaacggccag tctcctgaaa acgattgatt ccgaagccgc cgccggcgtc
     6961 gattcgctca ttcaagccgt tcgcagccgg ctggtccgcc atgtcggcgc gcagcagcaa
     7021 tatgacgata tgaccctgat cgcccttgaa gtccactagc ccgttccgtc ctccggcatt
     7081 gactagccgg cccaaatgcg tattttttca tgattcggaa ccattttaac cggattccgg
     7141 aagaaggatt ttaatgatga gtaaaaaaca caaaacagaa gaaaacaaac ctgcgcagga
     7201 acagccggaa acggttatcg aaccggccgc cgctgaaccg gctgtcatcc ggaaagaaga
     7261 cgaagaaccc ttgaaaaacc agatgctccg tctgcaggcg gacttcgata atttccgcaa
     7321 acgcacccag cgcgaacgcg gcgagctctt tcagatggcc aacgaaacgc tctttctcga
     7381 aatactgccg gtcatcgacc atttcgaaat ggggttcaaa agcgcggaag cgcataaaac
     7441 cgactgctcc gtcaccgaag gattccggat ggtttacaac cagatgctgg atgtcctgaa
     7501 aaaattcaac gtgacggtca tcgagacggt cggtgaatca ttcgatccgc accgccacga
     7561 atcgctcctg catatgccgt cggataagcc ggccggaacc gtcatcgaac agatccgccg
     7621 cggatacatg tttggtgaaa aactgctgcg cgccgcgcag gtcatcgtct ccagcggccc
     7681 tgccgaacaa aaggaatctg aataatgccc ggacagaaaa gagattatta cgaagtgctc
     7741 ggcatcgcca aaggcgcctc cgccgaagag atcaagaagg cctaccgcaa aatggccatt
     7801 cagtatcacc cggacaaaaa ccccggcgac aaggcctctg aagaaaaatt caaagaggtt
     7861 tccgaagcct acgaagtact cagcgacgct tcgaaacgcc agcagtatga ccagttcggc
     7921 cacgccgcct tcggcgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc
     8041 aacgcaccgc aggaaggcgc cgacttgcgc ttcgatctgg atatcgattt cgaagaagcc
     8101 gtcctcggtt cctcgcgcga aatccgcatc aacgtcaacg aaacctgcga acgctgcaaa
     8161 ggcagcggcg cagaacccgg ctccggacgc aaaacctgtt cgacctgccg cggcagcggc
     8221 caggtggtca gcggcggcgg gttcattcag ttccgccaga cctgtccggc ctgccgcggt
     8281 gcgggtcagg tgatcgaaaa gccctgcacc gcctgccacg gtcaggggct ggttcgcaac
     8341 aaacgcacca tcgacgtgaa gattccggcc ggagtcgaaa ccggctcgcg cctgcgcatt
     8401 gccggcaagg gcgaaggcgg actgcgcgga ggcccggcag gcgatcttta tattgtactg
     8461 cacgtccgcg aacacgaatt tttcaagcgc aacgatctgg atattgtctg cgaaattccg
     8521 gtgccgttcc atatcgccgc gctgggcggc gaagtccctg tgccgacgat tcacggcgga
     8581 gccgaactca aaattcctgc cggtacggaa agcggcaagg tgttccggat gaagggcaaa
     8641 ggcatcagca gtccgcgcta cggcaccggc gaccagcatg ttgtcgttca gatcgaaatt
     8701 ccgcaggggc tcggcagcaa agaaaagaaa aagctgcagg aagccgccga gatgctgaac
     8761 gagaagcact tcccgctgag acagaaaatg cataaagcgg cggaaaaatt ctacgagcgc
     8821 aaacggatcc tggaaaaagg ccaatgagga ttaacagctt cgcggacccg gcggcgctgc
     8881 gcgcggagcg cgtagtgctt gctgcggatg agtcgcacca ccttgcacgg gttctccgcg
     8941 tcgaggcggg tcaggagctg acgctgtttg acggagaggg aacccgcgcc gacggcgtgg
     9001 ttgatgccgt gaccaaaaaa gaggtgatcg tccggattac cgcgcgcgaa accgttccgc
     9061 cgccggcggt ggagattacg ctcattcaag cggtctgcaa accggaccgg ttcgagctga
     9121 tcctgcaaaa ggcgaccgaa ctcggcgtcc gccggattca gccggtcgtt acacaaaatg
     9181 ccagtctgcc cgccggcaaa atcgagaaga tgatggagcg cggcgtagcg attatccgca
     9241 atgcggcgca gcagtgcggt acggcctgga tgccggagct gcttgcgctg caaaaattca
     9301 gcgcactgtc cctgaaggcc gacgccgcgt ttatcggctc gctccacccg aatgcccgtc
     9361 cgttccgcga cgcgttccgg gcgctcgatg cctccgnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnngcct ggtcgatgtt taacaaaaac atcccgcttg aggagtataa
     9541 gaaaacggcc cacgaagtga cggcctccgg cttcaatgcc gaggaaatcg tcaaactggc
     9601 tgaagaaagc ggcatgcact acatcacctt tgtggccaag caccatgacg gcttctgcct
     9661 gtgggacagc caatacaccg actttgacag taaagactat ttcaaccgcg acctgctcgc
     9721 tgagctggcc aaagcctgcc atgaacacgg catgccgctc tgcatttact attccatcgg
     9781 cattgactgg acccacccct acttcctgcc gcgggaactc tattccgtgg gccgcccgaa
     9841 atatgatgcc gccggcaacg cgtacttcaa ataccgcgtt ccggaggact ttgagaacta
     9901 ccgtacgttt tgcaaacagc agctggctga gctgagcacc aagtacggtc cggtcgccgg
     9961 gttctggttc gacccgctgg gcggcgtact ggctaacagc gagatgttta aacttcagga
    10021 aatgtatgac gtcatccgtc aactccagcc gcaagccctc attcacttca agaccggcgc
    10081 aaccggcacc gaagatattc tggtcggcga acgctctctg gcgcccatca gctctttcta
    10141 taaaggcgat tccccgcaaa ataaacggat tcgcgagctg tccgacgccg cctggaaggc
    10201 gaacttcaga aagaaggctg aaattgccgt caccagtcag ggtccctggg aatggtcccc
    10261 gaaagtcacc tgccggccgg ccgacggcct ctggaagatg ctgctgatcg cggcggacaa
    10321 taatgcgaat ctgctgctca acttcggccc gaagcctgac ggctcgattc cggacgatgt
    10381 ggcaaccaac ttccgccagc tcggcgagcg gatccgcaaa gaaggctatc cgccgctcaa
    10441 caagaccacc tatctggaaa aacgtcagaa aggtgtcgtg gcggatgaca ccgaaaagga
    10501 aaagacggcc cgctgattaa cgttccgttc ttgcggatcg caggcggcgg ctcaacagcc
    10561 gccgcttttt ttataacatg acggagtaaa tctgccgtat tgcatcggcc ccccgtcttc
    10621 cggcatgatc gcacaggctg aatccaaaaa cagaagcggg aactgcattc atgagtacta
    10681 aagttattgc gctggcgaat caaaagggcg gcgtgggaaa aaccaccacc gccgtcaatc
    10741 tggctgccgc gctggccgaa cgccgtaaaa acgtattgct gatcgatctc gatccgcagg
    10801 ctaacgcaac aagcgcgctc ggtctggaga aagaggaagg cgccagtatt tattccgcgc
    10861 tgctcggcga ggcagacgtc cgcacgctga ttaagccgac agcgacgaag cggctgcaca
    10921 tgattccgtc ggaacttgat ctggcgggct gcgaggtggc gatcgcccgc atgaacaatt
    10981 atctgcactg cctgaagaac gctctgcgcc cgattgttga gcaggacgaa tatgacgtga
    11041 tcctgctcga ctgcccgccg tcgctcggca ttctcttcat gaatgcgctc tacacggctg
    11101 attcgattgt gattccgatt caggccgaat atctggcgct ggaaggcctg agcgtgatcg
    11161 tcagcg
//
LOCUS       DAOPZO010000056        11158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_6698_length_11158_cov_9.234171, whole genome shotgun sequence.
ACCESSION   DAOPZO010000056 DAOPZO010000000
VERSION     DAOPZO010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 11158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11158
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6698_length_11158_cov_9.234171"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(289..1092)
                     /locus_tag="PKI68_05065"
     CDS             complement(289..1092)
                     /locus_tag="PKI68_05065"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX53095.1"
                     /translation="MMLNDKIAVVTGGAQGLGEALCLRLAKEGCHVVIGDMNEQGAAE
                     TAISVEATGRNAFSMKVDVSKEEDVAALFKKAMDEFGRVDIVVANAAILIAEPIAEAN
                     AAKWQAVINVNLFGTFLTLKHAAKIMKAQKSGSMVVISSKSGKKGSAANSAYASSKFG
                     QIGLVQSVALELAPLGIRCNAVCPGNFLNGTLWMDPEKGLFKQYLEAGKVPGAKSIED
                     VKQCYMNQVPLRRGCEYEDVENVVVFLASNLASYMTGQAINVTGGQEMK"
     gene            complement(1107..2759)
                     /locus_tag="PKI68_05070"
     CDS             complement(1107..2759)
                     /locus_tag="PKI68_05070"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNX53096.1"
                     /translation="MKKIPSKQNAVQLIGPDELILNTSKDVHQPNDYQILCKVIVVGL
                     CFSDLKLLKQFSGHVRKSEVLSGIDPNVLKENPAYAPGELPTVPGHEPVVEIVKAGAK
                     VKNFKAGERYMIQADWRWLATASSNGAFGYNFEGALQEYVLLDERIVISPDGVSMLLP
                     APSGQRSASAFALSEPWACGEDAYQEVQRQGLKKGGRLLIVANTCGSKEEGQTFFAAQ
                     EKPAEIVKVGLAGGCKCEGALSSLDEVPDNSFDDIMYFGADAAVVEKIFAKGKKGALF
                     IICQCGQKFGRPVMTAVGGVHYRGFRIAGTAGSNPAAAVASIPERCGMRKGDKVNVIG
                     AAGPMGVTHVIRNLCVGNGVTVYAADLSDERLAALAKIAEPTAKAAGTTYIGYNPKVT
                     QPDVLFDMQVVMAPVPALVAAAVKTTAPKGIIDIFAGIPADKYGEIDLDAYCEKQLYF
                     IGTSGSTMDDIEIVLNHVAEGRIDTNVSVAAISGLDDAVLGIRKVEKQEVPGKIMVYP
                     SCKGLKLTLLSDLAKVLPEVADKLKNGVWTKEAEEALLAHFA"
     gene            3096..3172
                     /locus_tag="PKI68_05075"
     tRNA            3096..3172
                     /locus_tag="PKI68_05075"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3130..3132,aa:Arg,seq:tct)
     gene            3200..4294
                     /gene="alr"
                     /locus_tag="PKI68_05080"
     CDS             3200..4294
                     /gene="alr"
                     /locus_tag="PKI68_05080"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNX53097.1"
                     /translation="MNRSWVEIDLSRLKQNVRALRSSIPAETAVILVVKSDAYGHGLV
                     PVARAAAEEGINWFAVAFLEEALNVRAALPDANIVVLGPVLPEHTLELLEKRIFTVVT
                     CLEHGLALAEIARSEGKALPVHLKVDTGMGRLGVQWDEVAETVEAFNAAGGLDLAGVC
                     SHFARVEPDHPDHAAAQAAKFSEALKHLPAGVFKHLSSSRAALYFPGWDMDGVRQGIV
                     LYGYGANDPKGRFQTKPILQWKARVVQVKKVPADFAVGYYGTYRTEYPTQIATLAVGY
                     ADGYNRALSNKGDVLIGGRRCAVVGRVSMNWITADVGPDASVKVGDEVVLIGEQGEEA
                     IWADELSKICRTIPYEILTSINASLERKYV"
     gene            complement(4291..4500)
                     /locus_tag="PKI68_05085"
     CDS             complement(4291..4500)
                     /locus_tag="PKI68_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53098.1"
                     /translation="MFKPNRHAWLWWVSAVTLSIGISIYILLKPAVYDMEIQRYRSMA
                     LVISILISGLCIILGTSRRWFGKDL"
     gene            complement(4559..6322)
                     /locus_tag="PKI68_05090"
     CDS             complement(4559..6322)
                     /locus_tag="PKI68_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNX53099.1"
                     /translation="MKIPDFRLLIVFCLTVLSASGAEVTMTIQPPLIQLGESAVLSIE
                     VRGAKKLQPPALPNISGLRFASAGQSTHTSWINGKSDSFTAFNFQIYPQQTGTFTIGP
                     FEYKVDGETKTLQGELKVVATSGDASQPQSWNDLVFARLEADCKSAYVQEPFGLTLSI
                     YSRQGVQMAGNINLQGMPETGLSGFQWQEIQPDRQVISNAVYDVRKFHSRTRAVSSGV
                     FEFKPSITVQVVAQNQNNRSRSGFDDPFFGSMFQRTETRPVDLPVELVSILIRPLPEE
                     GKPAGFSGAVGQFGFQVSAQPLAVHPGDPITLTMVISGDGNFDRVTAPSLPANDLFRL
                     YGEPVRKQADNAVLFEQVISPRSAEAREIPSVAFSFFDTQSGTYQTVKSAPIPVTVTA
                     TSNSTAQVFAAKDSIVLPPQETPFATESDLQRIGAHLRNFWRHIHPWLWILPAVLVLS
                     TAVFSARKLRLSRQSDTARIRRQNAPKAARKALRAAEHARRKNDSGAFYDALWSALTA
                     YFGHRLNLPPGEVTSTAALQALTRSGFASEQVSTLQEIFEQVEASRYGFPGSGKSPDA
                     MEKLQSSLEQILKQCEKEKLR"
     gene            complement(6332..8107)
                     /locus_tag="PKI68_05095"
     CDS             complement(6332..8107)
                     /locus_tag="PKI68_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNX53100.1"
                     /translation="MRFGSPEFFKWLLLLLPLIALFVFMHRQRAARLSKLIAASIWKT
                     VIPGHREQRSRIRTGLRLLALLCIGLALTRPQWGSHWEEVKQRGLDLIVVLDTSKSML
                     AEDLKPNRLKQAQWAVRDFVKQLKGDRIGLVAFAGSSFLQCPATVDYAAFTMTLDDLY
                     AGIIPRGGTAIEQALKTAAESFDTKSESDRVILLITDGEDHEGDPLRMAEQLRKEGIK
                     LFAIGVGTLDGELIPAQAGYVKNPQGQVVKSALNEPLLEKLARETGGFYVRAAPGDFG
                     LDRIYKQGLSGLQRKEQETRLARVYEERFGWFAGAALLLLLAEGLFRPAALVVLLLLW
                     STPPAEAADWVKAYRQGDYTNALPALEKMSETFPDIASYNRGNVLYREKDYAAAEKAY
                     GSAAAQTASPEVRQQALFNRGNALLAGAVKQNGPDRTDAALSAATQAAGLFEQALALK
                     PSDLDAKQNLERALILKEKLEQQKKEQERQKQDQQKQKQEDDQKNPQQQDPQQKQDQP
                     KQPDEQNKPTDRPQEQQKPSDQANQNNQKQQQDRQQPDQPRRAGEMSEEEARQLLDAM
                     KQNEQDQRANLTPFLGGPIRVEKDW"
     gene            complement(8104..9075)
                     /locus_tag="PKI68_05100"
     CDS             complement(8104..9075)
                     /locus_tag="PKI68_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNX53101.1"
                     /translation="MRLAYPYLLLLLLLIPLLLWWRARRRGPAVNFPDGSLLQKLPVS
                     PAVHLQPLLTVFYVLGLICLIVALARPQKGLQESRVNTEGVEIVLLLDLSTSMETPDF
                     SRNSQQQTRIESAKQVISEFISKRKDDRIGMVGFAALPYSIAPLTLDHSWLVQRLGGL
                     RTGMLEDGTAIGDAIASAVNRLRDSKAKSRIVILLTDGMNNRGELTPENAAQAAAALG
                     IKIYTIGVGGGAPVQRGFFTLPPQEIDEATLRRIAELSKAEFFRARDLKTLEEVYARI
                     DQLEKTEIEMQQFTRFEEKAGGWLIAALLLLTLEKLLSLSRFGRLPE"
     gene            complement(9072..10001)
                     /locus_tag="PKI68_05105"
     CDS             complement(9072..10001)
                     /locus_tag="PKI68_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53102.1"
                     /translation="MKSVGLYFLILLLLTGCGKPVEPLPPQGFDARAVLSTNTLLVGD
                     PVTLTLTARHPAGSTIRFPDIGSGKETVVRGRSAASRDVSKTIVESEESYQLTSFRVG
                     DWTLTTNPVVCTLSGGEQKIQALPELVLHVQSSLNATNATKLADIKEIIKPPLQLPRK
                     LWIILLIALLALLAGILTLVLRRTSRAAEASAPPEPPHLRARRALDALRAKPWSPEPF
                     FTELALILRTYLENRFDLHAPESTTEELTRAMTSDARLELQQQQALQNFLTQADLVKF
                     ARAGAEQDVMQNAFATVESFVEQTRAEPQEADV"
     gene            complement(9998..10930)
                     /locus_tag="PKI68_05110"
     CDS             complement(9998..10930)
                     /locus_tag="PKI68_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNX53103.1"
                     /translation="MRARTGNQQSAISNSSANSHRDLLKKVRRIEIKTRHAVNDVFAG
                     RYHSVFKGRGMEFDEVREYVPGDDIRAIDWNVTARTGVPHIKKFIEEREMTVMLLIDI
                     SGSNDFGSTGQLKRDLAAEVAALLAFSATRNNDRIGLILFSDRVEKYIPPHKGTASHV
                     LRLIREVLYHEPQSRQTDARPALDFLNHTSHRKAVTFLISDFIFPVACEKELKITARR
                     HDLIAVAIADRHERAWPKAGVIEWCDPESGRRILVDTSSAATRSALLLEQERQRDTRQ
                     RALRRAGIDTIELFTGEPYEKAFMKFFRQRSRRR"
BASE COUNT         2138 a         3054 c         3388 g         2578 t
ORIGIN      
        1 ctggaaaaaa tgctcggcac cggacgtccc gccggccgcg tcgcttcgat tatcgccgaa
       61 gaactcggct gattttccaa tactcgggtt caatgaatac ggctggattg ccgagcgtgt
      121 caggtaaagc cggaacttag ggcgaagccc gtagatccgg cttacggacg ggctgatcag
      181 cgggaagttc cggacccacg ctgaatacag ctgagttccg gcagtacagg caaatcccgc
      241 cgcttataaa ataaaaacgg cgaagaggaa ttgccgtgct cgatttttct atttcatttc
      301 ctgaccgccg gtgacgttga tggcctggcc ggtcatgtag gaagcgaggt tggaggcaag
      361 aaagacgacg acgttttcaa cgtcttcata ttcgcagccg cggcgcagcg gaacctggtt
      421 catgtagcac tgtttgacgt cttcaatcga ttttgcgccc ggaacttttc cggcttcaag
      481 atactgtttg aagagtcctt tttccggatc catccagagc gttccgttga ggaagttgcc
      541 ggggcagacg gcattgcacc ggataccgag cggagcgagt tcgagcgcga cgctctgtac
      601 gaggccgatc tgcccgaatt tactggaagc ataggcggag ttggccgcgc tgcctttctt
      661 tccggatttg gagctgatga caaccatcga gccgctcttc tgtgccttca tgattttcgc
      721 ggcgtgtttg agggtgagga aggtgccgaa gaggttgacg ttgatgacgg cctgccactt
      781 ggcggcgttg gcctcggcaa tcggctcggc gatcagaatc gcggcgttgg cgacgacgat
      841 atcgacgcgg ccgaattcat ccatcgcttt tttgaaaagg gcggcgacgt cctcttcttt
      901 ggagacatcg actttcatcg aaaacgcgtt acgtccggtg gcttccacgc tgatggcggt
      961 ttccgcggct ccttgctcgt tcatgtcgcc gatgacgacg tggcagcctt ctttagcgag
     1021 gcgcaggcaa agggcttcgc caagaccctg agcgccgccg gtcacaaccg caattttgtc
     1081 gttgagcatc atgagtaaac tccttgttat gcaaaatgtg cgagcagggc ttcttcggct
     1141 tctttagtcc agacgccgtt tttaagttta tcagcgactt ccggcagtac cttggcgaga
     1201 tcggaaagca gagtcaattt cagtcctttg caggaaggat agaccatgat cttgccgggg
     1261 acttcctgct tctcgacttt acggatgccg agtactgcgt catcgagccc ggagatggcg
     1321 gcgaccgaaa cgttggtgtc tatgcggcct tccgcgacgt ggttgaggac aatctcaatg
     1381 tcgtccatgg tcgagccgga ggtgccgatg aagtagagct gtttttcgca ataggcatcg
     1441 agatcgatct cgccgtattt gtccgccgga atgccggcga aaatatcaat gatgcctttc
     1501 ggagcggtgg ttttgacagc ggcggcgacc agcgccggaa ccggggccat gacgacctgc
     1561 atatcgaaca gcacgtccgg ctgagtgact ttcggattgt agccgatgta agttgttccg
     1621 gcggctttgg cggtcggttc ggcaattttc gccagcgcgg cgaggcgttc gtcgctgagg
     1681 tctgcggcgt aaacggtgac gccgttgccg acgcagaggt tgcgaatgac gtgcgtaaca
     1741 cccatcggtc cggcggctcc gatgacattg actttgtcgc ctttgcgcat tccacagcgc
     1801 tccggaatgg aggcgacggc ggcggcgggg ttgctgccgg cggtgccggc gatgcggaag
     1861 ccgcggtaat gcacgccgcc gacagcggtc atgaccggcc ggccgaattt ctgcccgcac
     1921 tggcagatga tgaacagagc gccttttttc cctttggcga atattttttc gaccacggcg
     1981 gcatcggcgc cgaaatacat gatgtcgtcg aacgagttgt ccggaacttc gtccagactg
     2041 gacagggcgc cttcgcactt gcaaccgccg gcaagaccga ctttgacaat ctccgccggt
     2101 ttttcctgcg ccgcaaaaaa cgtctgacct tcttctttgc tgccgcaggt attggcgaca
     2161 atcagcaggc gtccgccttt tttcaggccc tgccgctgga cttcctgata ggcgtcttca
     2221 ccgcaggccc aaggttcgga aagggcgaag gcggaagcgg atcgctggcc ggacggagcc
     2281 ggcagaagca tggaaacgcc gtctggcgag atgacgatcc gttcgtcgag caggacatat
     2341 tcctgcagtg cgccttcaaa attataaccg aacgcgccgt tggaactggc ggtggccagc
     2401 cagcgccagt cagcctggat catatagcgt tcgccggcct tgaagttttt aaccttggct
     2461 ccggctttga cgatttcgac aaccggttca tggcccggaa ccgtcggcag ttcgccgggg
     2521 gcataagccg ggttttcttt gaggacattc ggatcgatac cgctgagcac ctctgactta
     2581 cggacgtgcc cggaaaattg tttgaggagt ttcaagtcgg aaaagcagag gccgacgacg
     2641 atgactttgc agaggatttg atagtcgttg ggctggtgga cgtctttaga ggtgttgaga
     2701 atcagttcgt ccggaccgat cagctgtacg gcattttgct tggaaggtat ttttttcatt
     2761 atcttttttt tcggtttcca aacaccagtc ataaggcatg tttttatttt cgcaaaccac
     2821 aattagcata ttcgttcgtg aataggaaat cagacatttg atttttttat tacaacaaca
     2881 gttggaaaat catcgaagtt tacgtctgtt gccggggatt ccatggctga gagaaaaggg
     2941 gacgccgttt tccggaaaac ggttttgttc tgcagatgaa tacaacgctg cgagttgctg
     3001 ctgtaaaaac ggaacattta tccgcttttt taaatgtgtg gaaactcccg cttgcacatt
     3061 cggtcatctt caataatcat tcgcaggttt tcagcacccc ggtagctcag ctggatagag
     3121 catcggattt ctaatccgac ggtcacaggt tcgaaccctg tccggggtac cattcagctt
     3181 ttgcattgga agatgtatca tgaaccgttc gtgggtggaa attgatctga gccggctgaa
     3241 gcagaatgtc cgggccttgc gttcctctat tccggcagaa accgctgtga ttctggttgt
     3301 taagtccgac gcttacggac acgggcttgt tccggtggcg cgtgctgccg ctgaagaagg
     3361 aattaactgg ttcgccgtgg cctttcttga agaagctctc aacgtccgtg cggcgttgcc
     3421 ggacgccaat attgttgtgc tcgggccggt tcttcctgaa catactctgg aactgctgga
     3481 aaagcgtatt tttaccgtag tgacctgtct tgagcacgga ctggcgcttg ctgagatcgc
     3541 ccggtctgag ggaaaagctc ttcctgttca cttgaaagtg gataccggta tggggcgtct
     3601 cggcgtgcag tgggacgaag tcgccgaaac agtcgaggcg ttcaatgctg ccggcggcct
     3661 ggacctggcc ggggtttgca gccattttgc gcgggtggag ccggatcatc cggatcatgc
     3721 ggcggcgcag gccgctaagt tcagcgaagc cctgaagcat ttgcctgccg gagttttcaa
     3781 gcatctttcc agcagccgcg cggcgcttta tttccccggc tgggacatgg acggcgtccg
     3841 gcagggtatt gtgctttacg gctacggcgc gaacgacccc aagggacggt ttcagacgaa
     3901 gccgattctt cagtggaaag cccgggtcgt gcaggttaaa aaggttcccg ccgattttgc
     3961 tgtgggttat tacggaactt accgcaccga atatcccacg cagattgcca cgctggcggt
     4021 cggttatgcc gacggctata accgcgcgct tagcaataaa ggcgatgtgc tgatcggcgg
     4081 gcggcgatgt gcggtcgtcg gacgtgtcag catgaactgg attacggcgg atgtcgggcc
     4141 ggatgccagc gtgaaggtcg gtgacgaggt tgtgctgatc ggagagcagg gcgaggaagc
     4201 gatctgggcg gatgagcttt caaaaatatg ccggacaatt ccctatgaaa tcctcaccag
     4261 tatcaatgcg tcgcttgagc ggaaatacgt ttagagatct tttccgaacc agcggcggga
     4321 tgtgccgagg atgatgcaaa ggccggaaat caggattgaa atgaccagtg ccattgaacg
     4381 gtaacgctga atctccatat cgtatacagc cggtttcagc agaatataaa tcgagattcc
     4441 aatggagagg gtcacggcgg acacccacca tagccacgca tgccgatttg gtttaaacat
     4501 aaacatcatg aatctacgca gactaccgtt ttgtcatttt cttacaagtg aaactttctc
     4561 accgcaactt ctctttttca cattgtttta atatctgctc cagactgctt tgcagctttt
     4621 ccatggcgtc cggcgatttt ccgcttcccg gaaaaccgta acggcttgct tcgacctgtt
     4681 caaagatttc ctgcagggtg ctgacctgtt cagaagcgaa gccggatcgt gtcagcgcct
     4741 gcaatgcggc ggtggaagtc acttctcccg gcggcaaatt gaggcggtgt ccgaaatacg
     4801 cggttaatgc agaccagagt gcatcataaa aagctccgga gtcatttttc cgtctggcat
     4861 gttcggcggc gcgcagtgct ttccgtgcgg ctttcggcgc gttctgccgc cggatccgtg
     4921 cggtgtcgct ttgacggctc aggcgaagtt tccgtgccga aaatacggct gtactcagca
     4981 ccagtaccgc cggaagaatc cagagccagg gatgaatatg ccgccagaag ttccggaggt
     5041 gcgcgccgat gcgctgcaga tcgctttccg ttgcaaacgg agtttcctgc ggcggcagaa
     5101 caattgaatc tttggccgca aacacctgtg cggtgctgtt ggacgttgcc gttaccgtga
     5161 ccggaatcgg ggcgcttttc accgtttgat atgttccgga ttgcgtgtca aaaaatgaga
     5221 aggcgacgga cggaatttct ctggcctcgg cagagcgcgg cgaaatcacc tgttcaaaca
     5281 gcactgcatt atcggcctgc ttgcggaccg gttctccgta gagccggaac agatcgtttg
     5341 ccggcagaga cggcgccgtg acccggtcga aatttccgtc gccgctgatg accatcgtta
     5401 aggtgattgg atcgccgggg tgaacggcga gcggctgggc gctcacctga aatccgaact
     5461 gtcctacagc gccgctgaaa ccggcgggtt taccttcttc cggcagaggc ctgataagaa
     5521 tggataccag ttcgaccggc aggtcaacgg ggcgagtttc agtgcgctgg aacatagagc
     5581 cgaagaaagg atcgtcgaat ccggaacgcg aacggttatt ctggttctgg gcgaccacct
     5641 gaacggttat agatggcttg aactcaaata cacccgaact gacagcccgt gtacgtgaat
     5701 ggaatttccg gacgtcatag acggcgttac tgatgacctg acgatccggc tggatttcct
     5761 gccactgaaa cccgcttaat ccggtttccg gcataccttg caggttaatg ttgccggcca
     5821 tttgcacgcc ttgccgggag taaatcgaca gagtcagccc gaacggttcc tgcacgtagg
     5881 cgctcttgca atctgcctca agacgggcga aaacaagatc gttccagctt tgcggctgtg
     5941 aagcatctcc ggatgtagcg accactttca attcgccctg cagggttttg gtttcgccgt
     6001 cgactttgta ttcaaacggg ccaatcgtga acgtgccggt ttgctgagga taaatctgaa
     6061 aattaaacgc cgtgaaactg tccgatttgc cgttgatcca gctggtatga gttgattgtc
     6121 cggcgctggc gaaccggagt ccggagatat tcggtaaagc gggcggctgc agttttttcg
     6181 cgccccgtac ttcaatgctc agcaccgccg attcgcccag ttgaatcagc ggcggctgaa
     6241 tggtcattgt cacctcagca ccggacgcac tcagtacagt cagacaaaaa acaatcagca
     6301 atctgaaatc tggaatcttc aattctcgct cctaccaatc tttttccacc cgtatcgggc
     6361 cgcccaaaaa aggcgtcaga ttcgctcttt gatcctgttc attctgtttc atcgcgtcaa
     6421 gcagctgccg ggcttcttct tcactcatct ccccggcccg gcgcggttga tcgggctgtt
     6481 gccggtcttg ctgctgcttt tgattattct gattcgcctg atcggatggt ttctgttgct
     6541 cctgcggccg gtcggtgggc ttattctgct cgtccggctg cttcggttga tcctgtttct
     6601 gttgcggatc ctgctgctga ggattttttt gatcgtcttc ctgtttctgc ttctgctgat
     6661 cctgcttctg ccgctcctgt tcctttttct gctgttccag tttttctttc agaatcagtg
     6721 cccgctccag attctgttta gcgtccaggt cggacggctt cagggcgagg gcctgttcaa
     6781 acagtccggc ggcctgtgtg gcggcggaca gcgccgcgtc ggtccggtcc ggaccgtttt
     6841 gcttgacggc cccggccagc agggcgttgc cgcggttaaa gagcgcctgc tgccgcactt
     6901 ccggactggc ggtttgcgcg gcggccgaac cgtaggcttt ctctgccgcc gcataatcct
     6961 tttcgcggta cagcacattg ccgcggttgt agctggcgat gtccggaaac gtttcgctca
     7021 ttttttccaa tgccggaagt gcattggtgt agtcgccctg gcggtaggct ttcacccagt
     7081 cggcggcttc ggcgggcggc gtcgaccaca gcagcaacag cacgacgagc gccgccgggc
     7141 ggaagagccc ttcggccagc agcagaagca gcgcggcccc ggcgaaccag ccgaagcgct
     7201 cttcatagac tctggccagc cgcgtctcct gttccttgcg ctgaagaccg gacagaccct
     7261 gcttataaat ccggtccaga ccgaagtcgc ccggcgcggc gcggacataa aaaccgccgg
     7321 tttcccgcgc cagtttttcg agcagcggtt cgttcagcgc gctcttcacc acctgtccct
     7381 gcggattttt aacatagcct gcttgagcgg gaatcagctc gccgtcgagc gtgccgacgc
     7441 cgatggcgaa gagtttgatg ccctctttgc ggagctgctc ggccatccgc agcggatcgc
     7501 cttcatgatc ttcgccgtcg gtgatgagga gaatcacgcg gtcggactcg gatttggtat
     7561 cgaaactttc cgccgcggtt ttgagcgcct gttcgatggc ggttccgccg cgcggaatga
     7621 ttccggcata aagatcgtcg agcgtcatcg tgaaggcggc gtagtcgacg gtggcggggc
     7681 attgcagaaa gctgctgccg gcgaaggcga ccaggccgat ccggtcgcct ttaagctgct
     7741 tcacaaaatc gcgcaccgcc cattgcgcct gtttgaggcg gttgggtttt aggtcttcgg
     7801 ccagcatgct tttcgaggtg tcgaggacga cgatcagatc caggccgcgc tgcttcacct
     7861 cttcccagtg cgagccccat tgcggacgcg tcagcgccag cccgatacag agcagggcga
     7921 gcagccgcag gccggtgcgg atccggctgc gctgttcgcg gtggcccgga ataacggttt
     7981 tccagatgct ggcggcaatc agtttcgaca gccgggccgc gcgctgccgg tgcataaaaa
     8041 caaacagcgc gatcagcggg aggagcagca gaagccattt aaaaaattct ggtgaaccga
     8101 atctcattcc ggaagcctcc cgaagcgtga gagtgaaagc agtttttcca gcgtcagcaa
     8161 gagcagcgcg gcgatcagcc agccgccggc tttttcctcg aagcgggtga actgctgcat
     8221 ttcaatttcc gttttttcca gctgatcgat ccgcgcataa acctcttcca gcgttttcag
     8281 atcccgggcg cggaaaaact ccgctttact cagttccgca atacgtcgga gcgtggcttc
     8341 gtcaatttcc tgcggcggca gcgtgaagaa tccgcgttga accggtgcgc cgccgccgac
     8401 accgatggtg taaatcttaa tgccgagcgc ggcggcggcc tgcgccgcat tttccggcgt
     8461 cagctcgccg cggttattca ttccgtcggt gagcagaatg acaatccggc ttttcgcctt
     8521 gctgtcgcgc agccggttga cggccgaggc gatggcgtcg ccgatggccg tgccgtcctc
     8581 cagcatgccg gtgcgcaggc ctcccaaccg ctgaaccagc cagctgtgat ccagcgtgag
     8641 cggtgcaatc gagtacggca gcgcggcaaa gccgaccatg ccgatgcggt catctttgcg
     8701 cttactgatg aattcgctga taacctgctt ggcggattcg atacgggtct gctgctgact
     8761 gttgcgtgaa aagtcgggcg tttccatcga cgtggagagg tcgagcagca ggacaatctc
     8821 tacgccttcc gtattcacgc ggctttcctg caatcccttt tgcgggcggg ccagggcgac
     8881 aatcaggcag atcagcccca gcacataaaa gaccgtcagc agcggctgca ggtgcacggc
     8941 cgggctgacc ggcagtttct gcagcagcga gccgtccggg aagttgacgg ccggtccgcg
     9001 gcggcgggcc cgccaccaga gcagcagcgg aatcagtaac agcagcagga gaagatatgg
     9061 ataggccagc ctcatacgtc ggcctcctgc ggttcggccc gggtctgttc aacgaaactc
     9121 tcgacggtgg caaacgcgtt ctgcatcaca tcctgttccg caccggcgcg ggcaaacttc
     9181 accaggtcgg cctgcgtcag aaagttttgc agcgcctgct gctgttggag ctccagccgg
     9241 gcgtcggacg tcatcgcgcg ggtcagctct tcggtggtgg actccggcgc atggaggtcg
     9301 aagcggtttt cgagataggt gcgcagaatc agcgcgagct ccgtaaagaa cggttccggc
     9361 gaccagggct tggcgcgcag cgcatccagc gctctgcggg cgcgcagatg cggcggttcc
     9421 ggcggcgcgg aggcttcagc cgcccgagac gtgcggcgca ggacaagggt gaggattccg
     9481 gcgagcagcg ccagcagagc aatcagcaat ataatccaca gtttgcgcgg gagctgcagc
     9541 ggcggcttga tgatctcttt gatgtccgcc agcttggtgg cgttggtggc attcagggag
     9601 ctctgaacgt gcagaaccag ttccggcagc gcctgtattt tctgttcacc gccggacaag
     9661 gtgcagacga ccggattggt ggtcagcgtc cagtcgccga cgcggaacga ggtgagctga
     9721 tagctttctt ccgactccac aatcgttttc gaaacgtccc ggctggcggc ggagcgcccg
     9781 cgcaccaccg tttccttgcc gcttccgatg tccggaaagc ggatggtgct gcctgccgga
     9841 tggcgggccg tcagcgtgag cgtgacgggg tcgccgacca gcagcgtgtt ggtactcagt
     9901 acggcgcggg cgtcaaaccc ttgcggcggc agcggctcga ccggctttcc gcagccggtg
     9961 agcagtagaa ggatcagaaa atagaggccg accgatttca tcttctgcgg ctccgctgcc
    10021 ggaaaaattt cataaacgct ttctcgtagg gctcgccggt gaacagttcg atggtgtcga
    10081 ttcctgcgcg gcgcagtgcg cgttgccgcg tgtcgcgctg tcgctcctgt tcgagcagca
    10141 gagcgctgcg tgtcgcggcg ctggaggtat cgacgaggat gcgccggccg ctctccgggt
    10201 cgcaccactc gatcacgccg gctttcggcc aggcgcgttc atggcggtcg gcgatggcga
    10261 cggcgatcag gtcgtgccgc cgcgcggtga ttttcagctc tttttcgcac gcgaccggga
    10321 aaataaagtc gctgatcagg aaggtgacag ccttgcggtg gctggtgtga ttgaggaagt
    10381 ccagcgcggg ccgggcgtcg gtctggcggc tctgcggttc gtggtagagc acttcgcgga
    10441 tgaggcgcag gacgtgcgac gccgtgcctt tgtgcggcgg aatgtatttt tcaacccggt
    10501 cggaaaacag gatgagtccg atgcggtcgt tgttgcgcgt ggcggaaaag gccagcaggg
    10561 cggcgacttc ggcggcgagg tcgcgcttga gctgtccggt gctgccgaag tcgttcgagc
    10621 cgctgatgtc gatgagcagc atgacggtca tctcgcgctc ttcgatgaat tttttgatgt
    10681 gcggcacgcc ggtgcgggcg gtgacgttcc agtcgatggc gcggatgtcg tcgccgggca
    10741 cgtattcgcg cacttcgtcg aactccatcc cgcgcccttt gaagacgctg tggtagcgcc
    10801 cggcgaagac gtcgttgacg gcgtggcggg ttttaatctc aatccgccgc accttcttca
    10861 gcaaatcacg atgtgagttt gctgaagaat tgctgattgc cgattgctga ttgccggttc
    10921 gtgctctcat tggttttctt ctgctttttt cctggatgcg gtcatcaaat ccgtttgatt
    10981 cataggcctc ctgcaagaaa gcgactgttt attggcaatc ggaaatcctc attcgtcagt
    11041 cggaaatgtt acggaacggg gatctggctg agaattttct cgatgagaat ttccggcgtc
    11101 agttcttcgg cttccgcttc gtaggagacg atgatgcggt ggcgcagaat gtcggggg
//
LOCUS       DAOPZO010000057        11152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_6705_length_11152_cov_10.382446, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000057 DAOPZO010000000
VERSION     DAOPZO010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 11152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11152
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6705_length_11152_cov_10.382446"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1505)
                     /gene="guaB"
                     /locus_tag="PKI68_05115"
     CDS             complement(15..1505)
                     /gene="guaB"
                     /locus_tag="PKI68_05115"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNX53104.1"
                     /translation="MSHNKYLDKFMSTFPFEGLTFDDVSLITQYADFLPGEADITSRL
                     TTNIKLNIPFLSAAMDTVTEADMAIAMALHGGIGVIHKNLDPERQAGDVARVKHYLNG
                     LIGKPVTFNINQTLAEIQQRRMEKGYSFSGFPIMDDNGNLAGILTTKDMKYAASNNVR
                     AGDIMTKEVLTADGDTDLRQAYEIMRKNKIGKLPLVKDGKLIGLYSYTDVADLIENTR
                     PLYNRDEHYRLRAAAGIGPGDYVRAEALAGAEVDVLVVDTAHGHSKGVIEMTAWVKKN
                     LPGIDVIAGNVATGEAALALRKAGADAVKVGIGPGSICTTRVVAGVGIPQISAIYNCA
                     KALEGSIPVVADGGVRHSGDIAKALVAGASSVMMGSVLAGTDEGPGEKILYEGRQFVV
                     YRGMGSLDAMKSREGSRQRYGQKDAGDDDLVPQGIEGVVPYAGSVSKVLKQYSGGLQA
                     SLGYCGCRTVPELQENGQFVRVSHAGALEAHAHDIKITKEAPNYRR"
     gene            1627..1944
                     /locus_tag="PKI68_05120"
     CDS             1627..1944
                     /locus_tag="PKI68_05120"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNX53105.1"
                     /translation="MLALHVSIQVKPECIERFIEATGENAENSLKEPGIARFDLVQQQ
                     DDPTRFMLVEVYRDAEAPLRHKETAHYARWCKTVESMMAVPRSRVLYTTVLPAHESGW
                     RSA"
     gene            1941..>2809
                     /locus_tag="PKI68_05125"
     CDS             1941..>2809
                     /locus_tag="PKI68_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNX53106.1"
                     /translation="MTFEFATAGRIVFGNGTLAQAAPAAAGFGRCALVVTGRNAERAR
                     PLLAALKKENVGAELFTVGGEPTVADALKAVERARAAQCDLVIGFGGGSVIDLAKAAA
                     ALLTNPGDPFDYLEVIGKGQPLTVRPAPCIAIPTTAGTGSEVTKNSVLSSPEHRVKVS
                     IRHPLMIPDLAIVDPECTLSAPPAVTAATGLDALTQLLEAFISRKAGPMTDGFCREGL
                     PRAVRSLRRAFENGSDLKAREEMALASLFGGLALANAGLGAVHGFAGPVGGMFGAPHG
                     MVCAALLPHTMKA"
     assembly_gap    2810..2909
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2910..3140
                     /locus_tag="PKI68_05130"
     CDS             <2910..3140
                     /locus_tag="PKI68_05130"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNX53107.1"
                     /translation="RLTEFARMTGGMTAEDGIKWIAGLCRDLQVPPLSALGVSEKDFP
                     ALIESSQRASSMKTNPVELTAGELEEILHAAM"
     gene            3159..4088
                     /locus_tag="PKI68_05135"
     CDS             3159..4088
                     /locus_tag="PKI68_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNX53108.1"
                     /translation="MNSYSKSINPSYVSLSGGGGFATVFEFMVSRFPRIPESVWRERI
                     ELNKVHFDDGEPIRLATPYPDRRRICYYREVIGEIKIPFEEEVIFENDDFLVADKPHF
                     LPVHPAGDYVNETLVTRLRAKYGYEELGSAHRIDRSTAGLVLCVKDRSKRALYHQMFK
                     DGAVRKIYLAAGRLPAETGRTHWHIKARMEPRDHFHMQIVSGGPVNSESLIDLIGRRD
                     GIGLFRLQPVTGKKHQLRVHLCEIGSGIIDDPLYTDCADAESDDYQRPMQLLAHRLEF
                     TDPVTGKRMEFKSRRSLRLFPEPQSFAAASPKS"
     gene            4200..5711
                     /locus_tag="PKI68_05140"
     CDS             4200..5711
                     /locus_tag="PKI68_05140"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNX53109.1"
                     /translation="MTAAGMATCAAWFGLCPRAEAQESVPKAVQPDILLIMPDQMRGD
                     CLSLLGHPAVRTPHLDQLAAQGTVFTRAYTTCPSCIPARYALLTGLYPQTSGVVGFAN
                     KPVDVPTLPALLTEAGYSTLLVGRYMHQKPQNNGYQQQILGSTYVAGDDYDNYLKQAA
                     PDSGGIRKLVTSKLRISYNSASAKPWPLAEELHPSVWIVRESCKAVEAAEPDRPLFLT
                     ASFYAPHPPLFPPARYFEAVKQRQLPEPAHGDWEEWSALSPAGNKAGNKAGVRILLQG
                     ALLRDVLCGYFGLIEQLDDQLVTLVDAFKERSRKAGRPWVIVFCSDHGEMLGDHGYFR
                     KCEPYEGSARIPLIIAGSPELGFRAGQRMAQPVCLEDILPTLLDLAQGQRPARLDGIS
                     LIPALRGDTQKIRPWVHLEHSPYYSREQAFHALTDGRFKYIWRPVSGAEQLFDLENDP
                     QENHDLARDAQAAPLLNEWRARMVQQLAGRPEGFSDGKKLIAGRPYPAIQKKP"
     gene            5844..6680
                     /locus_tag="PKI68_05145"
     CDS             5844..6680
                     /locus_tag="PKI68_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53110.1"
                     /translation="MLIVKTESSEVYRNLAVEEWLLGHAEGRGPILFLCVNSPCVVIG
                     KNQNPWRECRLSLMEEAGVPLARRISGGGAVYHDEGNLNVGVIVPRAEYCEEQQYELI
                     FNTLNRFGIKASRLLKNSLAVDGKKFSGQAFCFRGRHVLHHGTLLVNSDLGRLGRYLG
                     AEIEAIETNAVASVPAHVTNLAPFAPGLTVEKLSGALMETFRKMYGSTGVCEYGSDKE
                     IPEAELLPMIEKLSSADWQLRHTPRFQVTLDGQTLDVEKGRITNLAGIPLFEDFLTAN
                     GH"
     gene            complement(7073..9619)
                     /gene="galB"
                     /locus_tag="PKI68_05150"
     CDS             complement(7073..9619)
                     /gene="galB"
                     /locus_tag="PKI68_05150"
                     /inference="COORDINATES: protein motif:HMM:NF041463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase GalB"
                     /protein_id="HNX53111.1"
                     /translation="MKRQLGTIWAGLLLAGSALCAAPGRQVENFNADWRFERFGSLAD
                     GSTRPEPAGLEKAQADDQAWRKLDVPHDWGVEGPFRQELNGATGKLPWKGIGWYRKHF
                     TLTKADEGRRIFIELDGAMAHAQVWLNGQKVGEWPYGYTSFQLELTPFVRLGQENVLA
                     VRLDTDNWNSRWYPGAGIYRNVRLVKTDPVHVAHWGVQLTTPDLTDKQGPVQLAVQVQ
                     NQRPSGVEAQVQTAIYELSKDDVAGAQVALSAPVKLNLKAETNAVATLNATVAAPKRW
                     DLATPVRYLARTTVQVNGQVVDTVDTPFGFRTLKFTAHDGFYLNGRRVQVFGVCQHHD
                     LGALGAAVNLRALERQIEILQEMGCNAIRTSHNPPTPELLDLCDKMGVLVMVEAFDTW
                     HKSKKPKDYADLFAAWHEKDIRAMVRRDRNHPSVFMWSTGNEIPARETPEGMANSARL
                     TALVKEEDSTRPVTCGANSATKHMKNGFQTTMDIYGFNYAPSGYADFHKVPGNENRLY
                     IASETASTVSSRGEYFFPVATKFESAQRTTVKGNFQISSYDVQAPGWGCTPDIQFGAL
                     DSNPACLGEFVWTGFDYLGEPTPYNKDESNLLNFTGDPNKLAALKKQLDELGKIETPS
                     RSSYFGIIDLAGFKKDRFYIYQARWRPELPMAHLLPHWNWPERIGQVTPVHLYTSGDE
                     AELFLNGKSLGKKKRGPNDYRLRWDDVVYEPGTLKAVVYKNGKPWAEDTMKTTGPASR
                     LALSVDRSTLRADGQDLAFVTVDVCDDAGLMVPRSSNLVRFALEGPGRIIAVDNGDAA
                     SFEPFQADYRKAFNGKCLVIVRTQAGQAGKFVLKAESEGLKPATVEFQSK"
     gene            9843..>11152
                     /locus_tag="PKI68_05155"
     CDS             9843..>11152
                     /locus_tag="PKI68_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HNX53112.1"
                     /translation="MHVFEKLISTLSGWVWGPPMLILLVGTGLYLTIRLKGLQFRALP
                     MAFRMIWDKEHGHAGDISHFQALMTALAATVGIGNIVGVATAITLGGPGAVFWMWMTG
                     LVGMVTKYSEAVLAVKYREHGPHGMRGGPMYYISKGVGLPWLGTLFAIFTALATFGIG
                     NMTQANATVAIFQTVFHVEPWITGLVLMVLTALVIIGGIKSIGKFTSLLVPVMIFGYV
                     ISALVVLVLNAGEIPHAFALIFRHAFSPAAATGGFAGATIAAAMRFGIARGVFSNESG
                     LGSAPIAAAAARTHDPVKQALVSMTQTFIDTLVVCTMTALVILTATSWTQGVAPGELT
                     SASFAETLGGPGRIIVAISTALFAYSTLIGWNYYGEKAIEYLLGPRSIKIYRIVFIAM
                     VMVGSMIKLELVWNISDLMNGMMAIPNLIALLVLAKVIQSETDR"
BASE COUNT         1992 a         3201 c         3522 g         2337 t
ORIGIN      
        1 gcagagcgtt ttcattaccg ccggtagttc ggggcttcct tggtgatctt gatgtcgtgc
       61 gcgtgggctt cgagcgcgcc ggcgtgcgaa acgcggacga actgtccgtt ttcctgaagt
      121 tccggaacgg tgcggcatcc gcagtagccg aggctggcct gcaatccgcc gctgtactgc
      181 ttgagcactt tgctgacgga tccggcgtac ggcacaacgc cttcgatgcc ctgcggaacg
      241 aggtcgtcgt cgccggcatc tttctggccg tagcgctggc gggagccttc gcgcgacttc
      301 atggcgtcga ggctgcccat gccgcggtaa acgacgaact ggcggccttc gtagagaatc
      361 ttttcgcccg ggccttcgtc ggttccggcg agtacggagc ccatcatgac gctggaagcg
      421 ccggcgacga gcgccttggc gatgtcgccg gaatggcgga cgccgccgtc ggcaacgacc
      481 ggaatggagc cttcgagcgc cttggcgcag ttgtaaatgg cgctgatctg cggaatgccg
      541 acgcccgcga cgacgcgggt ggtgcagatg gagccggggc cgataccgac tttgacggcg
      601 tccgcgccgg ctttgcgcag ggcgagggcc gcttcgccgg tggcgacgtt gccggcgatg
      661 acgtcgatgc ccggcaggtt tttctttacc cacgcggtca tttcgatgac gcctttgctg
      721 tggccgtgtg cggtgtcgac gacgaggacg tcgacttcag cgccggcgag cgcttcggcg
      781 cggacgtagt cgccggggcc gatgccggcg gcggcgcgca ggcggtagtg ttcgtcgcgg
      841 ttatagagcg ggcgggtgtt ttcaatcaaa tcggcgacgt cggtgtagct gtaaaggccg
      901 atcagcttgc cgtctttgac gagcggcagt ttgccgattt tgtttttccg catgatttcg
      961 taggcctgac gcagatcggt gtcgccatcg gccgtgagaa cttcttttgt catgatgtcg
     1021 ccggcgcgca cgttgttgct ggcggcgtat ttcatgtctt tggtggtcag aattccggcg
     1081 aggttcccgt tgtcgtccat gatcgggaag ccgctgaagc tgtagccttt ttccatgcgg
     1141 cgctgctgga tttcggccag cgtctggttg atgttgaagg tgaccggttt gccgatcagg
     1201 ccgttgaggt aatgcttgac ccgcgctacg tcgccggcct gccgttcggg gtcgagattt
     1261 ttgtgaatca cgccgatacc gccgtgaagc gccatggcaa tcgccatatc ggcttcggtg
     1321 acggtgtcca ttgccgcgct gaggaacgga atgttgagtt tgatgttcgt ggtcagacgg
     1381 ctggtaatgt ccgcttcgcc gggcagaaag tcggcgtact gggtgatcag cgagacgtcg
     1441 tcgaacgtga gcccttcaaa aggaaaggtg ctcataaatt tatcgagata cttgttgtgg
     1501 ctcatggaat gtgtcctttt gccgcaacag cttggcgacc ggcggcggtg tagtcaataa
     1561 tgttttatga ttcgcaacgc cggcgcggct ccggtaggct tcagcgcatc agatcggagg
     1621 agttttatgc tggccttaca tgtgagcatc caggtcaaac cggagtgcat cgaacggttt
     1681 attgaagcga ccggagaaaa cgctgaaaac agtctcaaag aaccgggcat tgcgcggttc
     1741 gatctggttc agcagcagga cgatccgacc cgttttatgc tggtggaagt ttaccgcgat
     1801 gccgaagcgc cgctgcgtca taaagaaacc gcgcattatg cccgctggtg taaaacggtc
     1861 gagtctatga tggccgtgcc gcgcagccgc gtgctttaca caaccgttct gccggcacat
     1921 gaatccggct ggagaagtgc atgacttttg aatttgcaac cgccggccgg attgttttcg
     1981 gcaacggaac gctggctcag gccgcgccgg ccgccgcagg attcggccgg tgcgcgctgg
     2041 ttgtcaccgg acggaatgcg gaacgcgccc gcccgctgct cgccgcgctg aaaaaagaaa
     2101 atgtcggcgc ggaacttttt acagtcggcg gcgagccgac ggtcgctgac gcgctgaaag
     2161 ccgttgaacg cgcccgtgcg gcgcagtgcg atctggtgat cggcttcggc ggcggcagcg
     2221 ttatcgacct ggccaaagcg gcggccgcgc tgctgacgaa tcccggcgat ccgtttgact
     2281 atctcgaagt catcggcaaa ggacagccgc tgaccgtgcg gcccgcgccg tgcatcgcga
     2341 ttccgaccac cgccggaacc ggttccgaag tgacgaaaaa ctccgtactc tcttcgccgg
     2401 agcatcgcgt taaagtcagc atccgccatc cgctgatgat tcccgatctg gcgatcgtcg
     2461 atccggaatg tacgctttcc gcgccgcctg ccgtaaccgc cgcaaccggg cttgatgcgc
     2521 tcacgcaact gctcgaagct ttcatttcgc gcaaggccgg cccgatgacg gacggtttct
     2581 gccgcgaagg actgccgcgc gccgtccgct cgctgcgccg cgcctttgaa aacggttcgg
     2641 atttgaaagc gcgcgaggag atggcgctgg ccagtctgtt cggcgggctg gcgctggcta
     2701 atgccgggct cggcgcggtt catggatttg ccgggccggt cggcggaatg ttcggcgcgc
     2761 cgcacggaat ggtctgcgcc gcgctgcttc cgcatacgat gaaagctaan nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ggctgacgga gttcgcccgg atgaccggag
     2941 ggatgacggc cgaagacgga atcaaatgga ttgccggact gtgccgcgat ttgcaggttc
     3001 cgccgctgtc cgcgctcggt gtgtcggaaa aagattttcc tgcgctgatt gaaagttcac
     3061 agcgcgccag cagtatgaaa accaatccgg ttgagctgac ggccggtgaa ctggaagaga
     3121 ttctgcatgc cgccatgtga cggcaggatc cgcactttat gaattcatac agcaaatcca
     3181 tcaatccgtc ttatgtgtct ctttccggag gcggcggctt tgcgaccgtt tttgagttta
     3241 tggtgtcccg ctttccccgt attccggaat ccgtttggcg tgagcggatc gaactcaata
     3301 aggttcactt tgatgacggc gagccgatcc ggctggccac tccttatccg gatcgccgcc
     3361 gcatctgtta ttaccgcgag gtcatcggcg aaattaagat cccgttcgag gaagaggtga
     3421 tctttgagaa tgatgatttt ctggtggccg ataagccgca ttttctgccg gttcatcctg
     3481 cgggcgacta tgtaaacgaa acgctggtca cgcggctgcg cgccaaatat ggatatgaag
     3541 agcttggcag cgctcaccgg atcgaccggt caaccgccgg tctggtgctc tgtgtaaaag
     3601 accggtcgaa gcgcgctctg taccatcaga tgtttaaaga cggcgcggtc agaaaaattt
     3661 atctcgcggc gggaaggctc ccggcggaga ccggccgaac gcactggcac attaaggcgc
     3721 gaatggaacc ccgcgaccat tttcatatgc agattgtttc gggcgggccg gtcaattccg
     3781 aaagcctcat tgacctcatc ggacgccgcg acgggatcgg gctgttccgc ctgcagccgg
     3841 tgacgggcaa aaaacatcaa ctgcgcgtac acttgtgcga aatcggttcc ggcatcattg
     3901 acgacccgtt gtatacggat tgtgcggatg cagagtctga tgattatcag cggccgatgc
     3961 aattgctggc gcaccgtctg gaatttacag atccggtgac gggaaaacgg atggaattca
     4021 agagcaggcg gtcgctccgc ctgtttccgg aaccacagag ttttgccgcc gcctcgccga
     4081 agagctgaca aggtttgccc ttgttgccgg gcggatgttg tgtgatttta ggacgctttt
     4141 ttaaacggtc agggcatccg cgggggctca cgatgatgga cgtttcaaaa cggaattgga
     4201 tgaccgcagc cggtatggcg acttgcgccg cctggttcgg attgtgtccg cgcgcagagg
     4261 cccaggagtc ggtgccgaaa gcggttcagc cggacatcct cctcatcatg ccggatcaga
     4321 tgcgcggcga ctgtctctcg ttgctgggtc atccggcggt gcgcacgccg catctggatc
     4381 agctggccgc gcagggaacg gtgtttacgc gggcttacac gacctgcccg tcctgcatcc
     4441 ccgcgcgtta tgcgctgctg accggattgt atccgcagac gagcggggtg gtggggtttg
     4501 cgaataagcc ggtcgatgtg ccgacgctgc cggcgctgct gaccgaggcc ggctactcca
     4561 cgctgctggt cgggcgctat atgcatcaga agccgcaaaa caacggatac cagcagcaga
     4621 ttctggggtc gacctatgtc gcgggcgatg actatgacaa ctatctcaag caggcggcgc
     4681 cggacagcgg cgggatccgg aagctggtta cgagtaagct gaggatctcg tacaacagcg
     4741 cctcggccaa gccgtggccg ctggccgaag agctgcatcc ttcggtctgg atcgttcgcg
     4801 aatcctgcaa ggcggtggag gcggcggagc cggaccggcc gctgtttctc accgcatcgt
     4861 tttatgcgcc gcatccgccg ctttttccgc cggcccggta ttttgaggcc gttaaacagc
     4921 ggcagctgcc ggagcctgcg catggcgact gggaagagtg gagcgctctc tcgccggcag
     4981 ggaataaggc agggaataaa gcgggcgtcc gcattctgct gcaaggcgcc ctgttgaggg
     5041 acgtgctctg cggatatttc gggctgattg agcagctgga tgatcagctg gtgacgttgg
     5101 tggacgcgtt caaggagcgc agccggaagg ccgggcggcc ctgggtcatc gttttctgca
     5161 gcgaccacgg cgagatgctg ggcgaccacg gctatttccg caaatgcgaa ccgtacgaag
     5221 ggtccgcccg cattcctctg attatcgccg gatcgccgga gcttggcttc cgggcgggac
     5281 agcgtatggc gcagccggtg tgcctggaag acatcctgcc gacgctgctg gatctggcgc
     5341 aaggtcaacg ccccgcccgg ctggacggga tcagtttgat cccggctctt cggggcgaca
     5401 cacagaaaat ccgtccgtgg gtgcatttgg aacattcgcc gtactacagc cgggaacagg
     5461 cgttccatgc gctcaccgac ggccgtttca aatacatctg gcgcccggtc agcggtgcgg
     5521 agcagctgtt cgatctggag aacgatcctc aggaaaatca cgatctggcc cgcgatgcgc
     5581 aggccgcgcc gctcctgaac gaatggcgcg cgcggatggt tcagcagctg gccggccggc
     5641 cggaaggatt ttcggatggc aagaagctga ttgccggccg cccgtatccg gcgattcaaa
     5701 agaagcccta agcggtcggc cgtttctact aagcttccgc cggcgaatcc caaagaacgg
     5761 cgtccgggtt ccgggcgccg tcgccgtttc gcggcctgtt tttgaatcgc acatccggca
     5821 ttcggccggt agtctcacgc accatgctga tcgtcaaaac ggagagttcg gaggtttacc
     5881 gcaatctggc ggtggaagag tggctgcttg gtcacgccga agggcgcggg ccgatccttt
     5941 ttctgtgcgt caattcgccg tgcgtggtca tcggtaaaaa ccagaatccg tggcgcgagt
     6001 gccggctgtc gctgatggaa gaagccggcg tgccgctggc gcggcggatt tccggcggcg
     6061 gcgcggtcta tcacgacgaa ggcaacctga acgtcggcgt catcgtgccg cgcgccgaat
     6121 actgcgaaga gcagcagtat gagctgattt tcaacacctt gaaccggttc ggcatcaagg
     6181 cctcccggct tctcaaaaac tcgctggcgg tggacgggaa gaaattttcc ggccaggcgt
     6241 tctgttttcg cggccggcac gttttacatc acggaactct gctggtgaac agcgacctcg
     6301 gccggctggg gcgttacctc ggtgcagaaa tcgaagccat cgaaaccaac gcggtggctt
     6361 ccgttccggc gcacgtcacc aaccttgcgc cgttcgcccc cggtttgacg gtggaaaaac
     6421 tctccggcgc gctgatggaa acatttagga agatgtatgg gagtacgggt gtgtgtgagt
     6481 atgggagtga caaggagatt cccgaggcgg aactgctgcc gatgattgaa aaactgtcgt
     6541 ccgccgattg gcaacttcgc cacacgccgc gctttcaggt gactctcgac ggccagacgc
     6601 tcgacgttga aaaagggcgc attactaatc tcgctggcat cccgttgttt gaagatttct
     6661 taaccgcgaa tggacactaa ttaacgcgaa tgagaaggct taatacttag ctcaatcttc
     6721 gtgccgcaat tcatgcaatt gaattttcga tgcagaagga tatctgcgac cgcgaaaagg
     6781 gaatgccgaa gtggcatttt tattgcgcgc attaaacgct tcaggtggac gcaaatcaat
     6841 cgcggcaggg attttctggc aaccgagtaa agccggaact tagagcgaag ctcgtagatc
     6901 cggcttatgg tggcaggact tgccggaccc acgccgtagc cggctgagtt ccggcagtac
     6961 tttcgcgtcc attcgtgatt aaaaatccgc tgttcgaaga gcgggcgaaa cggcgttaaa
     7021 aaaacagccg ccggaatgtt ccggcggctg tactcagcag aggctgggtc ggttacttcg
     7081 actgaaactc caccgtagcc ggcttcaagc cttccgattc ggctttcagc acgaacttgc
     7141 ctgcctggcc ggcctgcgtg cggacgatga ctaggcattt gccgttgaag gctttgcggt
     7201 agtcggcctg gaagggctcg aagctggcgg cgtcgccgtt gtcgacggcg atgatccggc
     7261 cggggccttc gagcgcgaag cgcaccaggt tgctgctgcg cggaaccatc agcccggcgt
     7321 catcgcatac atcgacggtg acgaaggcca gatcctgacc gtcggcgcgc agggtgctgc
     7381 ggtcgacgga gagtgcgagc cggctggcgg ggccggtggt tttcatggtg tcttcggccc
     7441 acggtttgcc gtttttataa acgacggctt tcagggtgcc gggttcgtag acgacgtcgt
     7501 cccagcgcag gcggtagtcg ttcggcccgc gtttcttttt gccgagcgat ttgccgttga
     7561 ggaagagttc ggcctcatcg cccgaggtgt agaggtgcac tggcgtgacc tgtccaatgc
     7621 gctccggcca gttccagtgg ggcagcaggt gggccatggg cagttccggg cgccagcggg
     7681 cctgatagat gtagaagcgg tcttttttga atccggcgag gtcgataatg ccgaagtagc
     7741 tgctgcgcga aggcgtttcg attttcccca gttcatccag ttgctttttg agggctgcga
     7801 gtttgttcgg gtcgccggtg aagttcagca gattggattc gtctttgtta tacggggtgg
     7861 gttcgcccag atagtcgaag ccggtccaga cgaattcgcc caagcaggcc ggattgctgt
     7921 ccagtgcgcc gaactggatg tcgggggtgc agccccagcc gggcgcctgc acgtcgtagc
     7981 tgctgatctg gaagtttcct ttgacggtcg tgcgctgggc gctttcgaac ttggtggcga
     8041 ccgggaagaa gtattcgccg cgcgagctga cggtggaggc ggtttcgctg gcgatgtaga
     8101 ggcggttttc gttgccgggc accttatgga aatcggcata gccgctgggc gcatagttga
     8161 agccgtagat gtccatggtg gtctggaagc cgttcttcat gtgcttggtg gcggagttgg
     8221 cgccgcaggt gacgggccgc gtggagtctt cttctttcac cagcgccgtc aggcgggcgg
     8281 agttggccat cccttccggc gtttcgcgcg ccgggatttc atttccggtc gaccacataa
     8341 agaccgacgg gtggttgcgg tcccggcgga ccatggcgcg gatgtctttt tcgtgccagg
     8401 cagcgaacag atcggcgtag tctttgggct ttttcgattt atgccaggtg tcgaaggctt
     8461 cgaccatgac cagcacgccc atcttgtcgc acagatccag cagttccggc gtcggcggat
     8521 tatgcgaagt gcggatggcg ttgcagccca tttcctgcag gatttcaatc tgccgctcca
     8581 gcgcgcgcag attgacggcc gcgcccagcg cgccgagatc gtggtgctgg cagacgccga
     8641 aaacctgtac gcggcggccg ttcagataaa acccgtcgtg cgccgtgaat ttcagggtgc
     8701 ggaagccgaa cggcgtgtcg accgtatcaa ccacctggcc gtttacctga acggtggtcc
     8761 gtgcgagata gcgcaccgga gtcgccagat cccagcgctt gggcgcggcg accgtcgcgt
     8821 tcagggtcgc gacggcgttg gtttcggcct tcaggttgag cttcaccgga gccgaaagcg
     8881 ccacttgcgc gccggcgaca tcgtccttgg aaagctcata gatggcggtc tgcacctgcg
     8941 cttcgacgcc gctggggcgc tgattctgca cctgaaccgc gagctggaca ggaccttgct
     9001 tgtccgtcag atccggcgtg gtgagctgga cgccccagtg ggcgacatgc acgggatcgg
     9061 ttttgacgag gcggacgttg cggtagatgc cggcgccggg ataccagcgc gaattccagt
     9121 tgtcggtgtc gagccggacc gccaatacgt tttcctgccc gagccgcacg aacggcgtca
     9181 gctccagctg gaacgacgtg taaccgtacg gccactcgcc gaccttctgg ccgttgagcc
     9241 agacctgggc gtgggccatt gcgccgtcca gttcaataaa gatgcgccgg ccttcatccg
     9301 ctttcgtcag cgtaaaatgc ttccggtacc agccgatgcc tttccacggc agtttgccgg
     9361 tggcgccgtt gagctcctgc cggaaggggc cctcaacacc ccagtcgtgc ggaacatcca
     9421 gcttccgcca cgcctgatcg tccgcctgcg ccttttccag cccggcgggt tcggggcgtg
     9481 tcgagccgtc ggccagcgag ccgaagcgtt caaaacgcca gtctgcattg aaattttcaa
     9541 cctgacgccc gggtgcggcg cagagggcgg atccggccag cagcagcccg gcccaaatgg
     9601 ttcctaactg tcttttcatt acactactcc gttggaatga tgagtcaccg gtcactctgg
     9661 accttctaat aagctgtata agccgccgat taaatccggc aagcctgctg aatgcaatgc
     9721 cctaaaacaa aaaagagtcg ccgccggccg ctggttgcaa tcggcggttt gtgcgcttgc
     9781 cgaacccgtg ccggcggagt atcactctca gccaattcca tcacgagctt caggaggcgt
     9841 tcatgcatgt ctttgaaaaa ctgatttcta ctctgtccgg ctgggtctgg ggcccgccga
     9901 tgctgattct gctggtcgga accggcctct atctgaccat ccgcctcaaa ggcctccagt
     9961 tccgcgcgct gccgatggcg ttccgcatga tctgggataa agagcacggc cacgccggcg
    10021 acatcagcca cttccaggcg ctgatgaccg cgctggcggc aaccgtcggc attggcaaca
    10081 tcgtcggcgt cgccaccgcc attacactcg gcggccccgg cgccgtcttc tggatgtgga
    10141 tgaccggcct ggtcgggatg gtgacgaaat attccgaagc cgtgctggcc gtgaaatacc
    10201 gcgaacacgg accgcacggc atgcgcggcg gcccgatgta ttacatctcc aaaggcgtcg
    10261 gcctgccgtg gctcggcacg ctgttcgcca tcttcaccgc gctggcgacg ttcggtatcg
    10321 gcaacatgac gcaggccaac gccaccgtcg cgatttttca gaccgtgttt cacgttgaac
    10381 cctggatcac cggcctggtg ctgatggtcc tgacggcgct ggtgatcatc ggcggcatca
    10441 aatccatcgg caaattcacc tcgcttctcg tgccggtcat gatcttcggc tatgtcattt
    10501 ccgcgctggt ggtgctggtt ctcaacgccg gagaaatccc gcacgccttc gccctgattt
    10561 tccgccacgc cttttctccg gccgccgcca ccggcggttt cgccggggcc accatcgccg
    10621 ccgcgatgcg cttcgggatt gcccgcggcg ttttttccaa cgaatccggc ctgggttccg
    10681 cgccgattgc cgccgccgcc gcgcgtacgc atgacccggt caaacaggcg ctggtgagca
    10741 tgacgcagac gttcatcgat acgctggtcg tctgcaccat gaccgcgctg gtgattctga
    10801 cggcgacctc ctggacgcag ggggttgctc ccggcgaact gaccagcgcc agctttgccg
    10861 aaacgctggg cggccccgga cgtattatcg tcgcgatctc caccgcgctc ttcgcctatt
    10921 ccacgctcat cggctggaac tactacggcg aaaaagccat cgagtatctg ctcgggccgc
    10981 gctccatcaa aatctaccgc atcgtcttca tcgccatggt catggtcggc tccatgatca
    11041 aactggaact ggtctggaac atctccgacc tgatgaacgg catgatggcg atcccgaacc
    11101 tcatcgccct gctggtgctg gccaaagtga tccagtccga aaccgaccgc ta
//
LOCUS       DAOPZO010000058        10958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_6881_length_10958_cov_9.666422, whole genome shotgun sequence.
ACCESSION   DAOPZO010000058 DAOPZO010000000
VERSION     DAOPZO010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 10958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10958
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6881_length_10958_cov_9.666422"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1424
                     /locus_tag="PKI68_05160"
     CDS             <1..1424
                     /locus_tag="PKI68_05160"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNX53113.1"
                     /translation="SIATAAEFPKTVPIPAGSFEMGDHIGFVDTKHGGDETPIHTVRL
                     DAFEMGVYDVTTAQFCDFLNAALAQKQIEVRAGGVYLPGGGELLCETRTLSPYSRIGW
                     DGAKFSVLDGKEEHPVVCIRWEGAAVYCNWLSAQKNLPLCYDTKTWACDLNKSGYRLP
                     TEAEWEYAARGGLQNPYRNFPWGDDADASKANWPESKNKFRSGPEPWTTPVGYYTNGV
                     NGYGLYDMAGNVWQLVNDWYQSDYYAYSPASNPPGPASGKLMPDGKPYRGMRGGNWYN
                     GENGHSRVSNRNPSYWRGPLDPDHPYYHIGFRVARPVDAESRPEIKPTPVQNAARGDA
                     NRPKGGGQPPAGKQPAGKPDKPWLLEHATELDVNGDGAVARQEMLDEAVKAFGLYDTN
                     RDDVLDAAELQGGMAKTTMGGFIKLHSKELDENGDGTITKAEMLSVSGMLFDKSDKNR
                     DGRLTADEPSGGRKGEPKKAEGL"
     gene            1421..2920
                     /locus_tag="PKI68_05165"
     CDS             1421..2920
                     /locus_tag="PKI68_05165"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HNX53114.1"
                     /translation="MKKIMAGLSAAALCVCCAAGQEPVRSPETSYGDTVRQLGVIKNT
                     AEASPGYTLFAPKHYTSIYLVANDGQIVNEWKSQYEPGQSVYLLPNGHLLHCCLTKNK
                     SFIGGGEGGRLEEFDWDGKLVWEYVLSDDKNLMHHDIAPLPNGNILAMVVELKSLEEA
                     VKAGFNPDAVNDGKLFPDYIVEIQKTGPKSGKIVWEWHVWDHLVQNFDKTKDNYGDVA
                     KTPGRIDPNGSGRRVKAFWNHFNSIDYNAKLDQIVISARGNSEFWIINHGITTAEAKG
                     TKGDLLYRWGNPSTYQHNGKQMLFQQHDSQWTPDGCPGAGNILVFNNGLGRGYTSVDE
                     LTAPLNADGTYALKSDGTYGPDKLVWSYTDPDPKSFFSQEISSAQRLPNGNTLICAGV
                     HGTLFEVTPDGKTVWEYVNPVTTRVLKQGETVPLDHRYHAQNAIFKAQRYAPDHPAFA
                     GRELKPRGLLVENLANPVSPERRADQEHANMKPDDGGGAKPRDGKGARK"
     gene            2917..4779
                     /locus_tag="PKI68_05170"
     CDS             2917..4779
                     /locus_tag="PKI68_05170"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HNX53115.1"
                     /translation="MKKLIALVVLTAGISFAYESLQGPTEMLYWNKAKAFNGYTLFAA
                     HGSSYLIDMQGQLVHSWRIGTNPRFLQNGNLLDAMQDDPSSFKGFQEQDWDGNVIWKY
                     EEVRPNYAPHHDFVRIFKQKLNQWTTMYIAGKTVTREMALAAGCDPSKAPAAAGEEPG
                     RGSRLAKDGKTMVDAVVEVDMNGNVVWEWCFFDHLVQNVDPAKSNYVAKISDAPGRLN
                     MHLPGKPLRGDWLHCNSMDYNPELDQVVINSVQGEFYVIDHGATFVPGDPAKSIALAA
                     GPAGDFLYRFGDPARYEQGDPPSISENWTKGISGHKQIGGAHDVQWIPAGLPGAGHFL
                     IFNNGQYLFDPSAQSFVYEIDGFRKADGTASATYVNPPEAGYVQQDYHPDMQKTRRLI
                     SRQIVWLYGSKSSQGFFSSIGSGAQRLPNGNTLICSDAEGHFFEVTAQNELVWEYINP
                     VTKEFGVLKVMPDTIPMANSSFRCYRYSADHPALKGRDLTPKGLLTDVVVRPARKNPA
                     DEKFPQPGDAKSAPQQEEKPAARKAVAVATPAAATNSPAAAAPRPQGGGDRFAQMDAN
                     KDGAVSFDEMAAHEKAKRGDQYNEEQLRRKFEAMDADHNGLISKEEMDRAPKGK"
     gene            4787..5815
                     /locus_tag="PKI68_05175"
     CDS             4787..5815
                     /locus_tag="PKI68_05175"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNX53116.1"
                     /translation="MKTPVAIRLTAGLLLLNAANAEVLPCLPDTGQTNRYTQTFGEDS
                     DFRGRGPSYADNGDGTVTDRITGLVWQKTDGGEMTWEQAREYAHKLQLGGHADWRLPA
                     SMELFSVVDHSANGPAMNTAVFTRTEAKYWWTDSVRCDDTDRVWVVNSGGGIGPHLKQ
                     LATSPDKKGAFHVRCVRGDSPLRAGPSLNDNGDGTVTDQTTGLIWQKIGPDSDLTWEE
                     ALTYCSWLRLAGQSDWRLPNIKELRSLSDDRNVKPSVDKTFFPAVQAAYYWSSTSQCN
                     RPESAWFADFTTGLVSHTEKQQKHLVLAVRGGAAVPGPAVKPAPDPTALPGGRGEKRD
                     APQKERRP"
     gene            5817..7181
                     /locus_tag="PKI68_05180"
     CDS             5817..7181
                     /locus_tag="PKI68_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNX53117.1"
                     /translation="MKRIILSAMLLAVAAVPVFGKSSGDRKNQTAQDVPLMMEAGPAS
                     ADFECTELLAFPTETSIEIHLIPRRDMELSVRYGEGRLDQSTAPVSVRAGEPRVFRLE
                     NLKPDTEYMYRIEGEVHRFHTARPAGRSFAFAVQADPHLDENSNLAVYSNTLQNIAAA
                     RPDFLIDLGDSSMVDKLREKNNESIAARHLLMRNWYGQVAHSLPLFMVLGNHDGEQGW
                     LFDNTDKNSAVRATNIRKRFYPVAPETFYSSAAPDEKFCGTVRSYYSWTWGDALFVVI
                     DPYRYTMQKPGRKTDGWGFTLGETQYRWLRATLENSTAKTKFVFGHQLVGGGEQGRGG
                     VEAADWYEWGGRDPDGTYRFNEKRPGWGKPIHQLFVDTGVKVYFHGHDHFYARQEKDG
                     VIYQLVPQPASESVRAPGYAADYGYTTGDIRGGSGFLLVTVSPTGYEMKFVQSESPRK
                     GGKK"
     gene            7181..>8567
                     /locus_tag="PKI68_05185"
     CDS             7181..>8567
                     /locus_tag="PKI68_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNX53118.1"
                     /translation="MKKWIWAGAVLLTALSCWAEKPAKKEKTQGREGGGNRMAYPAPK
                     QAWDVATIILGIPEADRISLSIYPAAELEGYIEYGTNRTAPQLLSAKTPTQLTLTNLT
                     ADAEVAYRLRYRQPGDAEFKTGPAYRFHTARAPGRPFVFEIQGDSHPERSPKQNDPAL
                     YEQMLLAAAKDRPDFYICMGDDFSVDTLQTVNPETVRQVYLRQLPYLGLVAHSAPLFL
                     VNGNHEQAARANLDGSSNNVAVWAQNSRNRFFPQPAPDRFYSGDTNEVPHIGLLRDYY
                     AWTWGDALFVTLDPYWHSEQAVDNNFGGGKKQRDLWNIGLGDEQYRWLKKTLEESPAK
                     YKFVFAHHVNGTGRGGAKLATSYEWGDAGGLRSHRPGWELPIHQLMVKNQVTIFFQGH
                     DHLFAREKLDGLIYQTLPDPANRNTDLRNAEAYPGADLPDGSGRVRVSVGPEKVRVEF
                     ISSRLPQDATPE"
     assembly_gap    8568..8667
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8668..10911
                     /locus_tag="PKI68_05190"
     CDS             <8668..10911
                     /locus_tag="PKI68_05190"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HNX53119.1"
                     /translation="QQPSAVVWHEVNGVSIPVPPGVHPRLYLRAQDLPDLKERMNDPA
                     LKKTWDDLVKMQEDWKPEEIPQVKDFRFYFNQKGPTVQAELKALNYLLTKDTATGRSA
                     IAQSLAVLEGSTFESAGDISRGIGLYMVSGAMVYDWCYDLLTPEEKGRFVKEFLRLAK
                     MLECGYPPEKDCSIVGHASEWMIMRDLLSTGIAIYDEFPEMYNLAAGRFFSEHLVARN
                     WFYPAHAYHQGMSYLNVRFSNDLFALWILDRMGAGNVFNPGQQFVLYDMMYKRRPDGQ
                     ILAGGDVDSSRKKSKTYSLPALLAGSYYKDEYLNYEFLKDPKVEAHCKIFELLWRDTK
                     LGSRKPDELPLSRYSGSPFGWMIARTGWGAGSVIAEMKINEYSFLNHQHQDAGAFQIY
                     YKGPLAIDSGSYNGSSGGYNSPHNKNYFKRTIAHNSLLVYDPGETFRCWGYGGGNKTE
                     FAANDGGQRLPGKEWSAPRDLKEMLAGDFKTGKILAHGFGPDTQTPEYTYLKGDITEA
                     YSSKVKEVKRSFLFLNLKDEKIPAALIVFDKVVSANPDFKKFWLLHSIEQPEINGSQI
                     TIKRTKNGDSGMLVDTVLLPAPENADIVPVGGPGKDFWVFGTNYPNSPGAGSDEALER
                     GAWRVEVSPKKANAEDYYLNVMQMTDTARQQLHAVTRIDGDKITGVQIADRIVTFSKT
                     FQTVDRPFRLSVKGNGTFKFVLTDLLPGTWQILKDGKVYRPAVPVRSDDGILCFEGPA
                     GEYSFLR"
BASE COUNT         2379 a         3133 c         3409 g         1937 t
ORIGIN      
        1 catccatcgc cacggcggcg gagtttccta aaaccgtgcc gattccggcc ggcagttttg
       61 agatgggcga ccacatcggg tttgtggata cgaagcacgg cggcgatgaa acgccgattc
      121 atacggtgcg gctcgatgcg ttcgagatgg gcgtctatga cgtgacgacg gcgcagttct
      181 gcgattttct gaatgcggcg ctggcgcaga agcagatcga ggtgcgcgcc ggcggtgttt
      241 atctgcccgg cggaggcgag ctgctgtgcg agacgcggac gctgtcgccg tacagccgga
      301 tcggctggga cggcgcgaag ttttctgttc tcgacggcaa ggaagagcat ccggtggtct
      361 gcatccgctg ggaaggcgcg gcggtttatt gcaactggct gagcgcgcag aagaatctgc
      421 cgctgtgtta cgacacgaag acgtgggcgt gtgatttgaa taagagcggt taccgtctgc
      481 cgacggaggc ggaatgggaa tatgccgcgc gcggcgggct gcagaatccg taccgcaatt
      541 ttccgtgggg cgatgatgcc gatgcgtcga aggcgaactg gccggaatca aagaataagt
      601 tccgcagcgg gccggagccg tggacgacgc cggtcggcta ttacacgaac ggtgtgaacg
      661 gttacggtct gtatgatatg gcgggcaatg tctggcagct ggtgaatgac tggtatcaga
      721 gcgattacta cgcctacagt ccggcgagca atccgcccgg gccggcgagc ggcaagctga
      781 tgcccgacgg caagccgtat cgcgggatgc gcggcggcaa ctggtataac ggcgagaacg
      841 gtcacagccg tgtgtcgaac cgcaatccgt cctactggcg cggcccgctc gatccggatc
      901 atccgtatta tcacatcggt ttccgcgtgg cgcgtccggt ggatgcggag agccgtccgg
      961 aaatcaaacc gacaccggtg cagaacgcgg cgcgcggcga tgcgaaccgt ccgaaaggcg
     1021 gcggccagcc gccggcaggt aaacagccgg ccggcaagcc ggataagccg tggctgctgg
     1081 agcatgcaac ggaactggat gttaacggcg acggcgcggt ggcgcggcag gagatgctgg
     1141 atgaagcggt caaggcgttc gggctgtatg acacgaatcg ggatgatgtg ctggatgcag
     1201 cggaacttca gggcggcatg gcgaagacga cgatgggcgg ttttatcaag ctgcactcga
     1261 aggagctgga tgaaaacggc gacggaacga ttacgaaggc ggagatgctg agtgtctccg
     1321 gcatgctgtt tgataagtcg gataagaacc gcgacgggcg gctgacggcg gatgaaccgt
     1381 ccggcggacg caaaggggaa ccgaagaagg cggaggggtt atgaaaaaga tcatggcagg
     1441 attgagtgcg gccgcgctgt gcgtttgctg cgcggcgggg caggagccgg tgcgctcgcc
     1501 ggaaacgtcg tatggcgaca cggtgcgcca gctcggcgtg atcaagaaca cggcggaagc
     1561 ctcgccgggt tatacgctgt ttgcgccgaa gcattacacc agtatttatc tggtggccaa
     1621 cgacgggcag attgtgaatg agtggaagag tcagtacgag ccggggcagt cggtctatct
     1681 gctgccgaac gggcatctgc tgcactgctg tctgacgaaa aacaaatcgt ttatcggcgg
     1741 cggcgagggc gggcggctgg aagagttcga ctgggacggc aagctggtct gggaatatgt
     1801 tctttcggac gacaagaacc tgatgcatca cgacattgcg ccgctgccga acggcaatat
     1861 tctggcgatg gtggtggagc tcaaatctct ggaagaggcc gtgaaggcgg gcttcaatcc
     1921 ggacgcggtg aacgacggca agctcttccc cgactacatc gtggaaattc agaagaccgg
     1981 tccgaagtcc ggaaagattg tctgggaatg gcatgtgtgg gatcacctcg ttcagaattt
     2041 tgataagacg aaagacaact acggcgatgt tgctaaaacg ccgggccgga ttgatccgaa
     2101 cggttcgggc cgccgggtga aggcgttctg gaatcacttc aactcgattg attacaacgc
     2161 caagctcgat caaattgtca tcagtgcgcg cggcaacagc gagttctgga tcatcaacca
     2221 cggcatcacc accgctgaag ccaaagggac gaaaggcgat ctgctgtacc gctggggcaa
     2281 tccgtccacc tatcagcata acggcaaaca gatgctcttt cagcagcacg attcgcagtg
     2341 gactcccgac ggctgccccg gtgccggaaa tattctggtg ttcaacaacg gactgggccg
     2401 cggctacacc agcgtggatg aactgacggc tccgctcaac gcagacggca cgtatgcgct
     2461 gaagagtgac ggcacgtacg gcccggacaa gctggtctgg agttataccg atcccgaccc
     2521 gaagagtttt ttctcgcagg aaatttccag cgcgcagcgg ctgccgaacg gcaacacgct
     2581 gatctgcgcc ggcgttcacg gcacgctgtt tgaagtgacg ccggacggca agacggtctg
     2641 ggaatatgtc aatcccgtca ccacccgcgt gctgaagcag ggtgaaaccg ttccgcttga
     2701 tcaccgctac catgcgcaga acgccatctt caaagcgcag cgctatgcgc ccgatcatcc
     2761 ggcctttgcc ggacgcgaac tgaaaccgcg cggcctgctg gttgaaaatc tggcgaaccc
     2821 ggtttcgccg gagcgccgcg cagatcagga acacgccaat atgaagccgg atgacggcgg
     2881 cggcgccaag ccgcgcgacg ggaaaggagc ccgcaaatga aaaagctgat tgcgctggtt
     2941 gttctgaccg ccggtatttc gttcgcctat gaatccctgc aggggccgac ggagatgctt
     3001 tactggaata aagcgaaagc cttcaacggc tatacgctgt tcgcggcgca cggcagcagc
     3061 tacctgattg atatgcaggg gcagctggtc cactcctggc ggatcggcac caatccgcgc
     3121 tttctgcaaa acgggaatct gctcgacgcg atgcaggacg atcccagctc gttcaaagga
     3181 tttcaggagc aggactggga cggcaacgtc atctggaaat atgaagaagt ccgcccgaac
     3241 tatgcgccgc atcacgactt tgtgcgcatc ttcaaacaga agctgaatca gtggacgaca
     3301 atgtacatcg ccggtaaaac ggtcacacgc gaaatggcgc tcgccgccgg atgcgatccg
     3361 tccaaagcgc cggcagccgc cggcgaagaa ccggggcgcg gcagccggct ggcgaaagac
     3421 ggcaagacga tggtcgatgc cgttgtggaa gtggacatga acggaaacgt tgtctgggaa
     3481 tggtgcttct tcgatcacct cgttcagaac gtcgatccgg cgaaatccaa ttacgtcgcg
     3541 aagatttcgg acgcgccggg acggctcaat atgcatctgc cgggcaagcc gctgcgcggc
     3601 gactggctgc actgcaactc gatggactac aatccggagc tcgatcaggt ggtcatcaac
     3661 tccgtgcagg gcgagttcta tgtgattgat catggcgcga catttgttcc cggcgatccg
     3721 gcgaagagta ttgcgctggc ggccggaccg gcgggcgatt ttctttaccg cttcggcgac
     3781 ccggcccgct atgagcaggg cgatccgccg agcatttccg aaaactggac aaaaggaatt
     3841 tccggccaca aacagatcgg cggcgcgcac gatgtacagt ggattcccgc cggacttccc
     3901 ggtgcggggc atttcctgat ctttaacaac gggcagtatc tgttcgatcc gtccgcgcaa
     3961 tcattcgtct atgagatcga cggcttccgc aaagcggatg gaacggcgag cgcgacctat
     4021 gtgaacccgc cggaggccgg ttacgtgcag caggattatc atccggacat gcagaaaacc
     4081 cgccggttga tctccaggca gattgtctgg ctgtacggtt cgaaatcgag ccaaggcttt
     4141 ttcagcagca tcggttcggg tgcgcagcgc ctgcccaacg gcaacacgct gatctgctcg
     4201 gatgccgaag gccatttctt cgaagtgacg gcgcagaacg aactggtctg ggaatacatc
     4261 aatccggtca ccaaagaatt cggcgtgctc aaagtgatgc ccgacaccat tccgatggcg
     4321 aactcttcgt tccgctgcta ccgctacagc gcggatcatc ccgcgctcaa agggcgcgac
     4381 ctgacgccga aagggctgct gaccgatgtg gtggtgcgtc cggcgcgtaa aaatccggcg
     4441 gacgaaaaat tcccgcagcc cggcgatgcg aagagcgcgc cgcagcagga agaaaaaccg
     4501 gccgcccgca aagctgtcgc cgttgcaacg cccgcggcgg cgaccaacag cccggccgca
     4561 gcggcgcccc ggcctcaggg cggcggcgac cgctttgcac agatggatgc caataaagac
     4621 ggcgcggttt ctttcgatga aatggccgcg cacgaaaagg cgaaacgcgg cgaccagtac
     4681 aatgaagaac agctgcggcg taaattcgag gcgatggacg ccgatcacaa cggcctgatt
     4741 tcaaaagaag aaatggatcg cgcgccgaaa ggtaaatgag gacggcatga aaaccccggt
     4801 tgccatacgt ctgacggccg gccttctgct gctgaacgcg gcgaacgccg aagtcctgcc
     4861 gtgtctgccc gacaccgggc agaccaaccg ctatacgcag acgttcggcg aggattccga
     4921 tttccgcggc cgcgggcctt cgtatgccga taacggcgac ggcacggtga cggaccgcat
     4981 cacgggactt gtctggcaga aaaccgacgg cggcgaaatg acgtgggagc aggcgcgtga
     5041 gtatgcgcat aaactgcagc tgggcggcca cgcagactgg cggctgcccg ccagcatgga
     5101 gctgttcagc gtggtggatc acagcgcgaa cgggccggcg atgaacaccg ccgttttcac
     5161 gcggaccgaa gcgaagtact ggtggaccga ttcagtccgg tgcgacgata ccgaccgggt
     5221 gtgggttgtg aattccggcg gcggcatcgg cccccatcta aaacagctgg cgacgagccc
     5281 cgataaaaaa ggcgcctttc atgtccgctg cgttcgcggc gactctcctc tccgcgccgg
     5341 accgagcctg aatgacaatg gcgacggcac ggtgacggat cagaccaccg gcctgatctg
     5401 gcagaagatc ggcccggatt ccgatctgac gtgggaagag gccttgacct attgcagctg
     5461 gctgcggctc gcgggccagt ccgactggcg gctgccgaac atcaaagaat tgcggtcgct
     5521 cagcgacgac cggaacgtga aaccgtcggt ggataaaaca ttttttccgg cggtgcaggc
     5581 ggcctattac tggtcctcca cgagtcagtg caatcgtcct gaaagcgcct ggtttgcaga
     5641 tttcacaacc gggcttgtca gtcataccga aaaacagcag aagcatctgg tgctggctgt
     5701 gcgcggcggc gcggcggtgc cgggccccgc cgttaaaccg gctcctgatc cgacggcgct
     5761 tcccggcggg cgcggagaaa agcgggacgc tccgcaaaaa gaaaggcggc cctgaaatga
     5821 aacggataat tttgtccgcc atgctgttgg cggtcgcagc tgttccggtt tttggaaaat
     5881 cttccggcga ccggaaaaat cagaccgcgc aggatgttcc gctgatgatg gaagcgggcc
     5941 cggcgtcggc tgattttgaa tgcacggaac tgctggcgtt tccgacggag acgtcgatcg
     6001 agattcatct gataccgcgc cgcgacatgg agctgtcggt gcgttacggc gaaggccggc
     6061 tggatcaaag cacggcgccg gtgagcgttc gcgccggaga gccgcgtgtt ttccggctcg
     6121 aaaatctgaa gccggacacc gaatacatgt accggattga aggcgaggtg catcgttttc
     6181 acaccgcgcg tccggcgggc cggtcgttcg ccttcgcggt gcaggccgac ccgcacctcg
     6241 atgaaaactc caatctggcg gtctattcca acacgctgca gaatatcgcg gcggcgcggc
     6301 ccgattttct gatcgacctc ggcgattcgt cgatggtgga taagctgagg gagaagaaca
     6361 acgaatcgat tgcggcgcgt catctgctga tgcgaaactg gtacgggcag gtcgcgcaca
     6421 gtctaccgct gtttatggtg ttgggaaatc acgacggcga gcagggctgg ctgtttgaca
     6481 acaccgacaa aaattccgct gtgcgcgcga cgaatatccg caagcggttt tatccggtcg
     6541 cgcccgaaac gttttacagc tccgccgcgc cggatgaaaa attctgcggc accgtgcgca
     6601 gctattattc gtggacgtgg ggcgacgcgc tgttcgtggt gatcgatccg taccgctaca
     6661 cgatgcagaa gcccggccgc aaaaccgacg gctggggatt cacgctcggc gaaacgcaat
     6721 accgctggct gcgcgcaacg ctcgagaaca gcaccgcgaa aacgaaattt gttttcgggc
     6781 accagctcgt cggcggcggc gagcagggac gcggcggggt cgaagcggcc gactggtatg
     6841 aatggggcgg gcgcgatccg gacggcacct accggttcaa cgaaaagcgc cccggctggg
     6901 gcaagccgat tcatcagctg ttcgtggaca ccggcgtgaa ggtttatttc cacggccacg
     6961 atcattttta tgcacggcag gaaaaagacg gcgtcatcta tcagctggtg ccgcagcccg
     7021 cgagcgagag cgtgcgcgcc cccgggtatg ccgccgacta cggctacacg accggcgata
     7081 ttcgcggcgg ctcaggcttc ctgctggtga cggtttcgcc gacgggatat gaaatgaaat
     7141 ttgttcaaag cgagtcgccg cgcaaaggag gaaaaaaatg atgaagaagt ggatttgggc
     7201 gggtgcggtg ttgctgacgg ccctcagttg ctgggctgaa aaaccggcga agaaggaaaa
     7261 gacgcagggc agagagggcg gcggcaaccg gatggcctat cccgcaccca agcaggcgtg
     7321 ggatgtcgcg acgatcatcc tcggcattcc ggaagcggac cggatcagcc tgagtattta
     7381 tccggccgcg gagctggaag gctacattga atacggcaca aaccgcaccg cgccgcagct
     7441 gctgtcggcg aaaacgccga cacagctgac gctgacgaat ctgacggcgg atgcggaggt
     7501 cgcctaccgg ctgcgctacc gccagccggg cgacgccgag ttcaagacgg ggccggcgta
     7561 ccgatttcat accgcccgcg ctccgggccg tccgtttgtg ttcgagatcc agggcgactc
     7621 tcatccggag cggagcccga agcagaacga cccggcgctg tatgagcaga tgctgctggc
     7681 cgccgccaag gaccgcccgg acttctacat ctgcatgggc gacgatttca gcgtggatac
     7741 gctgcaaacg gtgaacccgg aaaccgtccg gcaggtttac ctccggcagc tgccgtatct
     7801 cgggctggtc gcgcattccg ccccgctgtt tctggtcaac ggcaatcacg agcaggccgc
     7861 gcgcgccaat ctcgacggaa gctccaataa cgtcgcggtc tgggcgcaga acagccgcaa
     7921 ccggttcttt ccgcagcctg cgccggaccg gttttacagc ggcgacacca acgaagtgcc
     7981 gcacatcggc ctgctgcgcg actattatgc ctggacgtgg ggcgacgccc tgttcgtcac
     8041 cctcgatccc tactggcact ccgaacaggc ggtcgacaat aatttcggcg gcggcaaaaa
     8101 gcagcgcgac ctgtggaata tcgggctcgg cgatgaacag taccgctggc tgaagaagac
     8161 gctcgaagag agtccggcga aatataaatt tgtcttcgcg catcacgtca acggaaccgg
     8221 gcgcggcggc gcaaaactcg caacctccta cgaatggggc gatgccgggg gcttgcgcag
     8281 tcaccggccc ggctgggaac tgccgatcca ccagctcatg gtcaaaaacc aggtcaccat
     8341 cttcttccag gggcacgacc atctcttcgc ccgcgaaaaa ctggacggcc tgatttatca
     8401 gaccctgccg gacccggcga accgcaatac cgatctgcgc aatgcggagg cctatccggg
     8461 cgccgacctg ccggacggct ccggccgggt ccgggtttcc gtcggcccgg aaaaagtccg
     8521 cgtggaattc atcagctcgc ggctgccgca ggatgcaacg ccggagcnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnncag caaccgtccg ccgtggtctg gcacgaggtc
     8701 aacggcgtat ccatcccggt gcccccgggc gtccatccgc gactttatct gcgggcgcag
     8761 gatcttcccg atctcaagga gcggatgaac gatccggcgc tgaaaaaaac ctgggacgat
     8821 ctggtgaaaa tgcaggagga ctggaagccc gaagagatcc cgcaggtcaa agacttccgt
     8881 ttttatttca accagaaagg cccgaccgtt caggcggaat taaaggcgct gaattacctt
     8941 ttgaccaaag ataccgcgac cggccgcagc gccattgccc agagccttgc cgtgctggag
     9001 ggctcgacgt tcgaatccgc gggagacatt tcgcggggca tcggtctgta tatggtttcg
     9061 ggcgccatgg tgtatgactg gtgctacgac ctgctgacgc cggaagaaaa aggccgcttt
     9121 gtgaaggagt ttttgcggct ggcgaaaatg ctcgaatgcg gctacccgcc ggaaaaggac
     9181 tgctccattg tcggccacgc ctccgagtgg atgatcatgc gcgacctgct ttcgaccggc
     9241 attgccatct acgacgaatt cccggagatg tacaatctgg cggccggccg gttcttcagc
     9301 gaacacctgg tggcgcgcaa ctggttttat ccggcccacg cctatcacca gggaatgtcc
     9361 tatctgaacg tgcggttttc caacgacctc ttcgcactgt ggatcctcga ccggatgggc
     9421 gccggcaatg tgttcaaccc cggacagcag tttgtcctct acgacatgat gtataagcgc
     9481 cggccggacg gacagattct ggcgggcggc gatgtggatt cgtcccgaaa gaaatccaaa
     9541 acctattcgc tgcccgcgct gctggccggc agttactaca aggacgaata cctgaactat
     9601 gaatttttaa aagaccccaa agttgaggcc cactgcaaaa tcttcgagct tctgtggcgc
     9661 gacaccaaac tgggcagccg caaaccggac gagctgcctc tttcgcgcta ctccggctcg
     9721 ccgttcgggt ggatgatcgc ccgcaccggc tggggcgccg ggagcgtgat cgcggaaatg
     9781 aagatcaatg aatacagttt cctgaaccat cagcatcagg acgccggcgc tttccagatt
     9841 tactataaag gcccgctggc cattgattcc ggttcgtaca acggttcttc gggcggctat
     9901 aacagcccgc acaacaaaaa ctatttcaag cgcaccatcg cgcacaacag cctgctggtt
     9961 tacgaccccg gcgaaacctt ccgctgctgg ggatacggcg gcggcaataa aacagaattt
    10021 gccgccaacg acggcggcca gcgcctgccc ggtaaagagt ggagcgcgcc ccgcgacctc
    10081 aaagaaatgc tggccggtga tttcaaaacc ggaaaaattc tggcccacgg gttcggcccc
    10141 gacacccaaa ccccggaata cacctacctg aaaggcgaca tcaccgaagc ctattcctcc
    10201 aaggtgaaag aggtgaaacg gtctttcctc ttcctgaacc tgaaggatga aaaaattccg
    10261 gcggcgctga ttgttttcga caaagtggtt tccgcaaatc ccgacttcaa aaagttctgg
    10321 ctgctgcaca gcattgaaca gcccgaaatc aacggcagcc agatcaccat caagcgcacg
    10381 aaaaacggcg acagcgggat gctggtcgac accgtactcc tgcccgcccc ggaaaatgcc
    10441 gacatcgtgc cggtaggcgg ccccggcaaa gatttctggg tgttcggcac caactatccc
    10501 aacagtccgg gagcgggctc tgacgaggcc ctcgaacgcg gcgcctggcg ggtcgaagtc
    10561 tcgcccaaga aagcgaacgc cgaagactat tacctgaacg tgatgcagat gaccgacacg
    10621 gcccggcagc aactgcatgc cgtcacgcgc atcgacggcg acaagatcac cggcgtgcag
    10681 atcgccgacc ggatcgtcac cttcagcaaa acgtttcaga ccgtcgaccg gccgttccgt
    10741 ttgtctgtta aaggaaacgg aacctttaaa tttgtgctga ccgaccttct gcccggcacc
    10801 tggcagatcc ttaaagacgg caaggtctat cggcctgccg ttccggtgcg cagcgacgac
    10861 ggaattcttt gcttcgaagg ccccgccgga gaatacagct tcttgagata gaaccgcctt
    10921 ccgcagcttg aaaacagaaa gccctgccgg aattttcc
//
LOCUS       DAOPZO010000059        10949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_6887_length_10949_cov_11.329998, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000059 DAOPZO010000000
VERSION     DAOPZO010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 10949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10949
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6887_length_10949_cov_11.329998"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..77)
                     /locus_tag="PKI68_05195"
     tRNA            complement(1..77)
                     /locus_tag="PKI68_05195"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Pro,seq:tgg)
     gene            181..951
                     /locus_tag="PKI68_05200"
     CDS             181..951
                     /locus_tag="PKI68_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNX53120.1"
                     /translation="MNDTLIIIPTYNERENVRAIAAAVFASSPDVDLLFVDDNSPDGT
                     GAIADEMAAADPRVKVMHRAEKNGLGRAYIAGFKWALERQYEFICEMDADFSHNPDDI
                     PTLRAAAKTAGLVLGSRYVNGVRVINWPLRRLVLSRGAGVYVKFVTGMSFTDPTGGFK
                     CFRRCVLETIPLDQIESSGYSFQVEMTHMAWRIGCRIVEVPIVFEERRSGASKMSKNI
                     IFEALIMVWKLYFRARFSRAPVCRVQPGGARFKCCQPR"
     gene            complement(929..1762)
                     /gene="rsmA"
                     /locus_tag="PKI68_05205"
     CDS             complement(929..1762)
                     /gene="rsmA"
                     /locus_tag="PKI68_05205"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNX53121.1"
                     /translation="MNTPGKLTSPSIIRALLDSLGHRPNKGLGQNYLIDSNILGIIIE
                     AANVSPSDQLLEIGPGLGALTQALMATGATLTAIEKDQTMSAHLRTHFPQLPLIDADV
                     LDVNLSELFAGGINKIVANLPYSVGSRFIVNALEARPLPERMVFMVQKEVADRLTAQP
                     GGKDYGPLAIWSQLNYEVKTIKNVSPTCFMPAPKVWSAVVRFEKRTVPLAEVKDYEQF
                     KKLIKLSFTQRRKQIGSNLRKNYPEFFPGLETCGIDPATRPEQISVKQWVALANAADS
                     I"
     gene            complement(1759..2679)
                     /gene="pdxA"
                     /locus_tag="PKI68_05210"
     CDS             complement(1759..2679)
                     /gene="pdxA"
                     /locus_tag="PKI68_05210"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNX53122.1"
                     /translation="MSIRIGITCGDVNGIGPEIALKAVSSSHWKPGIEFVLIGPEQVF
                     DHSSVWPVEFTGTQTPGKITAGASKTAVAAIERAVHGCLEGELDAMVTAPICKEGLKL
                     AGVNYPGHTEMIAELTGTNRYGMMLMGNGLRVMLVTRHLPLRSVADSLTKENVLEAIE
                     LTGEALNWFGLHNGRIGVCGLNPHAGDGGALGDEESAVIAPAIAAACAKGFNAAGPVP
                     ADVIFYQTLGKQYDAVVAMYHDQGLGPLKMHAFDCGVNLTLGLPIIRTSPDHGTAFNI
                     AGKGIAKPDSMIAAIETAIRLAGQPNPWKK"
     gene            complement(2688..3692)
                     /locus_tag="PKI68_05215"
     CDS             complement(2688..3692)
                     /locus_tag="PKI68_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNX53123.1"
                     /translation="MKNIVLFLALAAPALFAQTNVTLVTTATNSPKTVVTNGPLVTAI
                     DGYAARVDGTIITYGEVRESVAPYMQQISRKYKGKELAERIQAAYLDAREALIEEALL
                     KAETKTLKATLPDKVIDDEINRLIRERFNNDRALLSRALAERRMTYEEWKQEVTDQIT
                     VRIFYNQEVSRRASVPAQAVRDEYERNKEQYFVPFRVKYHFILISKGKTEEDRAVKRK
                     QAENTLQKLRAGADFIATAKEVSESDIDEAPWRNPDDVRAELRPALLNTPAGQISDLI
                     ETSGGFYIVKVEERREKGYTPFEEVQKAIEKKLLDAEKDRLHDDLLKRVSAKHFIERY
                     "
     gene            complement(3776..4957)
                     /gene="galK"
                     /locus_tag="PKI68_05220"
     CDS             complement(3776..4957)
                     /gene="galK"
                     /locus_tag="PKI68_05220"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNX53124.1"
                     /translation="MNKETAIQKTVAAHIENFGKKPESIAWAPGRIEVLGNHTDYNAG
                     TVLSAAINYGHCFCISRSDRPGIHLFAADAGQRASFDITDTGKVSGIGSGNYVKGVFH
                     FIQDHLKQKIDGLDCTSFGIVPLGAGLSSSAALEVSAAYAVLKILNVTVDKKEIARLC
                     QKAEHQFAGTKCGLLDQFSSIFGRNHGLIHSDFRTLEVSSVTLPDDIEFLVVNPHVKH
                     SLADSPYNERRERCEQAAAQLAEILPHPVAALRDVSPEEFEANRSRIAPGAAKRAAHI
                     IGEIDRVERGVELLRTGNIKAFGQLLFESHRSSIENFENSCPELDIVVEAARAAGAPG
                     ARLSGGGFGGCAIVMVQKNQAPAVTEKIGVSCREKGISPEFLAVTPAGGAELVSPESI
                     R"
     gene            5049..5900
                     /locus_tag="PKI68_05225"
     CDS             5049..5900
                     /locus_tag="PKI68_05225"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HNX53125.1"
                     /translation="MLATLNHTISQKVAEVFDLYTELHGARVSFFGPDKQMLYPDEKG
                     RPNCAYCRMLRETLKLDSRCRALDRQMMDAALTKNEMISYTCHAGMREAVAPLVVNSE
                     LAGFVMIGQFRSKEAADVSPYAVRWKKEQGNDALQTAFADSTVFPEEKIEILLAMLRQ
                     VLELIIRGQLIHRNDYDLIEPVIAQIRRQPDKNITLEAAARISGRSPSTVTRMFKKVT
                     GQSFKQYQVQFRLKRAAELLVDRPGRPVAEIAAAAGFEDPFYFSRQFHKHTGLSPSNY
                     RNSKAGE"
     gene            5902..6951
                     /locus_tag="PKI68_05230"
     CDS             5902..6951
                     /locus_tag="PKI68_05230"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HNX53126.1"
                     /translation="MAADKTAFGKNQFGVNVELFTLTNENGIRVRIMNHGGTIVSLEV
                     PDRSGRLADVVLGHDTAADYCAATPYFGCAVGRYANRIQGGRFTLDGQTYTLAQNNGP
                     NALHGGVRGFDKVVWDAEILPGADAVRMTYISADGEEGFPGEVKTTLTYTLTGANELK
                     IDCMAETDKATPYNITNHSYFNLAGHDAGEIGAQRMMIRAETLLPIDSTSIPLGPEMA
                     VAGTPFDFRTPHALGERIDADHEQIRRGCGYDHNFCLTKTRPGELSLAARAEDPASGR
                     VMEVYTTEPGVQLYTANFLDGQLAGKGGCRYVRRGGFCLETQHYPDSPNQPQFPNTIL
                     RPGTVFRSQTVYQFK"
     gene            complement(6979..8523)
                     /locus_tag="PKI68_05235"
     CDS             complement(6979..8523)
                     /locus_tag="PKI68_05235"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNX53127.1"
                     /translation="MRRLITVFLVSLCAFTAFAAVQPAGVFTDGAVLQQGEPVRIWGT
                     AAAGEKVTVLFADQTEETVADETGRWMVALKAMPACAEGRTLVLKGSQTAAPVELKDI
                     LVGEVWLAGGQSNMQYSMKGFKPTTQPDINSANDPLLRMVTIAEKLYESQPSARPQWA
                     ASTPDNVANFSASAYYFAKNLRETLKVPVGIVSCSLGGTPAEAWMSRKTLENHPDLKR
                     TIETYDQNYRANFTNEADYVKYLEAYPAAVKEWERKQAAGEKPGPKPDMKIGPRFKNR
                     PCGLHENMLSQTIPYTVKGVIWYQGENNAANGFHYRTVFPALIKEWREEFRNENLPFL
                     FVQLAAYGPATEKSAAWPELRESQSWTEDHVQNSGMIVLTDGGDIKNIHPHSKDKVGY
                     RLSLLARQMVYGETNLICRGPRLAKAEAKSGAIELSFKDTGSGLVLKPEAVSAFEICG
                     ADGTYVPAAARLADGKILVSAAGVASPKFVRYGWKKWFVPTLFNAEGLPASPFRTDDF
                     PPTTRR"
     gene            8667..9581
                     /gene="era"
                     /locus_tag="PKI68_05240"
     CDS             8667..9581
                     /gene="era"
                     /locus_tag="PKI68_05240"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNX53128.1"
                     /translation="MDETENSEMRAGVVAVVGRTNVGKSTLLNALVGEKVSIVSDTVQ
                     TTRNIIRAILTEPRGQIVFLDTPGIHKAEGDLGKNLNKMARSSVNGADAVLLVLDASS
                     KPWQEDDGWMRRLAAETTPCAILLNKTDLGTSFVETYKTLWNEIATEKNSTVQPVWIE
                     GSALKGGGLPELAAALFALVPEGPPMFPEDILTDYPRKLNMADVIREKYFDRLHDELP
                     HCLAIWVETIEETPAAWSIQAEVLVERHSQKGIVIGEKGRLLKSVQYQSEKELTEMYG
                     LPVKVKLWVKVEPNWRKNFWILKKLGYA"
     gene            complement(9628..10428)
                     /locus_tag="PKI68_05245"
     CDS             complement(9628..10428)
                     /locus_tag="PKI68_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53129.1"
                     /translation="MTTKTKKVLLITGASLLILVVALMLSLGRIIKTGVNTAGPKLAG
                     VPVHLDKVVVNPFTGLVQIKGLVIGNPEGFNTPSAMELNDFKLKIKMPSLFSKAIVIE
                     QILIDAPQITYEKSLKSSNLSTLQANLTPKETAAPKTETAPAAKPAEKKAAKKVIIND
                     FQLNGAKVNVSFTALGGKKLTLPLPDIHMKDIGKESQGASPAEVISEVFNSITKAVTE
                     AASSVGDAAGKALKDAGGAATDAAKGATDAAKGAVDSVKKGLGGLLGK"
     gene            complement(10560..10904)
                     /locus_tag="PKI68_05250"
     CDS             complement(10560..10904)
                     /locus_tag="PKI68_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53130.1"
                     /translation="MKTLIKIVLLSVLLSSVYGCASSGQKFETRLEPPKAFNPIFLKY
                     KEMPGQKVMVVAVDPTGQWAFGYEHSRATLEEAAETAAVKCDLARAENHVFTEAKLFA
                     VNDEVVYYKNLK"
BASE COUNT         2162 a         3025 c         3317 g         2445 t
ORIGIN      
        1 tggtcgggga aacaggattc gaacctgcga cttcttggtc ccaaaccaag cgctctacca
       61 gactgagcta ctccccgcac acaaaggcgc ggtaatatca gcgaagcttc cggtaaacgc
      121 aatattaaac cggcttcagc ttgcgtgaat gacctctttt caagtaggct ctgcggcatt
      181 gtgaatgaca cgctgatcat cataccgact tataacgagc gcgaaaacgt acgcgctatt
      241 gccgcggctg tcttcgcaag ctcccccgac gtcgatcttc tttttgtcga tgacaactcc
      301 ccggacggaa ccggcgctat tgcggacgaa atggcggcgg ccgatccgcg tgtgaaggtg
      361 atgcaccgtg cggaaaagaa cggtctgggc cgtgcatata tcgctggttt caaatgggcg
      421 cttgagcgcc agtatgagtt tatttgcgaa atggatgcgg atttttccca caaccctgat
      481 gatattccaa cgttgcgcgc cgccgctaaa acagcggggc ttgttctcgg ttcgcggtac
      541 gtcaatggcg ttcgcgtgat caactggccg ttgcgccggc tggttctgag ccgcggcgcg
      601 ggcgtgtacg tcaaatttgt caccggcatg tcgttcacgg atccgaccgg cgggtttaaa
      661 tgtttccggc gctgtgtgct ggagaccatt ccgctggatc agatcgaatc gagcggctat
      721 tcctttcagg tcgagatgac gcatatggcc tggcggatcg gctgccggat tgttgaggtt
      781 ccgattgttt tcgaagagcg ccgttccgga gcgtcaaaaa tgtcgaagaa cattattttt
      841 gaggcgctta ttatggtctg gaagctttat ttccgcgcgc gtttcagccg tgcgccggtc
      901 tgccgggttc agcccggcgg cgcccgcttc aaatgctgtc agccgcgtta gccagcgcga
      961 cccactgttt aacggaaatc tgttccgggc gtgttgccgg atcgattccg cacgtttcta
     1021 atcccgggaa aaactcagga taatttttcc ggaggttgga gccgatctgc ttgcggcgct
     1081 gtgtaaaact cagcttgatc agttttttaa actgctcgta atccttcacc tcagccagcg
     1141 gtacggtgcg tttttcgaag cggaccacgg ccgaccacac tttcggcgcg ggcataaagc
     1201 aggtcgggct cacattctta atggttttca cttcgtagtt cagctgcgac cagatcgcca
     1261 gcgggccgta gtctttgccg cccggctggg cggtcaggcg gtccgccact tccttttgca
     1321 ccatgaagac catccgttcc ggaagcggac gcgcttccag cgcgttgacg atgaaccggc
     1381 tcccgacgga atagggcagg ttggcgacaa ttttattgat gccgccggcg aacagttcgc
     1441 tcaggttaac atccagaacg tcggcatcga tgagcggaag ttgcggaaag tgggtgcgca
     1501 gatgcgccga catggtctga tctttttcaa tcgcggtgag cgttgcgccg gttgccatga
     1561 gcgcctgggt cagcgcgccg agaccggggc cgatttcaag cagctggtcg gacggggaaa
     1621 cgtttgcggc ttcaatgatg atgccgagaa tattggaatc aatcaggtaa ttctggccca
     1681 gccctttgtt cggacggtga ccgagcgaat cgagcagcgc acggataatg gaagggctgg
     1741 tgagtttgcc gggagtattc atttcttcca cgggttgggt tggccggcga gccggatggc
     1801 ggtctcgatt gcagcgatca tgctgtccgg tttggcgatg cctttgccgg cgatattaaa
     1861 tgcggtgccg tggtcgggcg aagtgcggat gatcggcagg ccgagggtca ggtttacacc
     1921 gcagtcgaag gcgtgcattt tgagcgggcc gaggccctga tcgtgataca ttgccacaac
     1981 ggcgtcgtat tgttttccca gtgtctgata aaaaattaca tcagccggaa ccggaccggc
     2041 ggcgttgaac ccttttgcgc acgcggccgc gatggccggg gcaatgacgg cagattcttc
     2101 gtcgccgagc gcgccgccgt cgccggcgtg cgggttgagg ccgcagacgc cgatccgccc
     2161 gttatggagt ccgaaccagt tcagcgcttc gccggtcagc tcaatggctt ccaaaacatt
     2221 ctctttagtc agtgaatctg cgacggagcg cagcggcaga tgccgcgtga caagcatgac
     2281 gcgcagtccg ttgcccatga gcatcatgcc gtagcggttg gtgccggtga gttccgcgat
     2341 catttcggtg tggcccggat aatttacgcc ggcgagtttc aggccttctt tacagatcgg
     2401 cgcggtcacc attgcgtcga gttctccttc gaggcatcca tgcacggccc gctcaatggc
     2461 ggcgacggcg gtttttgatg ctccggccgt gatttttccc ggagtctgtg ttccggtaaa
     2521 ctctaccggc cagacactgg aatggtcgaa tacctgctca gggccgatca gaacaaactc
     2581 aatgccgggt ttccagtggc tggaagaaac agctttgagg gcaatctcag ggccgatgcc
     2641 gttaacatcg ccgcaggtta tgccgatgcg gatgctcata ggtattttca gtagcgctcg
     2701 ataaaatgtt tggcagagac ccgtttgagc agatcgtcgt gcagccggtc tttttcggcg
     2761 tcgagcagtt tcttctcgat cgctttctgc acctcttcaa agggcgtgta gcccttttcg
     2821 cgccgctcct cgactttgac gatgtagaat cctccggagg tttcgatcag gtcactgatc
     2881 tgtccggcag gggtgttaag cagcgcgggg cggagttctg cgcgcacatc atcggggttc
     2941 cgccacgggg cttcgtcgat gtcgctttcg gaaacctctt tggccgtggc aatgaaatcg
     3001 gctccggcgc gcagtttctg caaggtgttt tcggcctgtt tgcgttttac ggcgcggtct
     3061 tcttcggttt tacccttact gatgaggatg aaatgatatt taaccctgaa ggggacgaaa
     3121 tactgctcct tattgcgctc atattcgtcg cgtacagcct gtgccggtac gctggcgcgg
     3181 cgggagactt cctgattgta gaaaatccgg acggtgatct ggtcggtcac ttcctgtttc
     3241 cattcttcat aggtcatgcg ccgttcggcc agcgcgcggc tgagcagggc gcggtcatta
     3301 ttgaaccgct cgcggatgag ccggttgatc tcgtcgtcga tcaccttgtc gggcagcgtg
     3361 gctttcagcg ttttcgtttc ggctttgagc agagcctctt caatgagcgc ctcgcgcgcg
     3421 tcaagatagg cggcctgtat gcgttcagcg agttccttgc ctttatattt gcgggagatc
     3481 tgctgcatgt aaggggcgac gctttcgcgg acttcaccgt aagtaatgat tgttccgtcc
     3541 acgcgggccg cgtagccgtc gatggcagta accagcggac cgttagtgac gaccgttttc
     3601 gggctgttgg tggcggttgt cactaaggtg acattggttt gcgcgaaaag cgccggagcc
     3661 gccaaagcaa ggaacagaac gatgtttttc ataagggcgg cattatgcac aaaccgtctg
     3721 ccgtgtgcaa cggctgttcg ctttcctttg ccggccgtat cgcgtgttcc gctttttagc
     3781 ggatactttc cggagaaaca agttccgcgc cgccggccgg tgtaaccgcc agaaattccg
     3841 gagagattcc tttctcgcgg caggaaacgc cgattttttc cgttactgcc ggtgcctggt
     3901 ttttctgcac catcacaatc gcacagccgc cgaagccgcc gccggaaagc cgtgcaccgg
     3961 gcgcgccggc tgcgcgggcg gcctcgacaa cgatgtccag ttccggacag gagttttcga
     4021 aattttcgat cgagctccgg tgcgattcga aaagaagctg tccgaacgct ttaatgtttc
     4081 cggtgcggag cagttcaacg ccccgttcaa cgcggtcaat ttcgccgatg atgtgggcgg
     4141 cgcgtttggc ggcgccgggc gcgatccggc tccggttcgc ctcaaactct tcgggcgaaa
     4201 cgtcgcgcaa ggcggcgacc gggtgcggca ggatctctgc cagttgcgcg gcggcctgtt
     4261 cgcagcgttc gcgccgttcg ttgtacggcg aatcggcgag gctgtgtttg acatgcgggt
     4321 tgacgacgag aaattcaatg tcatccggca gcgttacgga agagacttcc agcgtccgga
     4381 aatctgaatg gatgaggccg tggttgcgtc cgaagatgct ggaaaactga tcgagcagtc
     4441 cgcatttggt gccggcaaac tggtgttccg ccttctgaca gaggcgggcg atttcttttt
     4501 tatcgacggt gacgtttaga attttgagta cggcatacgc agcggagact tccagcgcgg
     4561 cggagctgga aaggcctgcg ccgagcggaa cgataccgaa ggacgtgcag tcgaggccgt
     4621 cgattttttg tttcagatga tcctgaataa aatggaagac gcctttgaca taatttccgc
     4681 ttccgatgcc ggaaacttta ccggtatctg tgatgtcaaa gctggcgcgt tgtccggcgt
     4741 ccgcggcgaa caggtgaatg ccggggcggt cggagcggga gatgcagaag cagtggccgt
     4801 agttgatggc ggctgaaagc acggtgccgg cattatagtc ggtgtggttg cccagcactt
     4861 caatgcggcc gggagcccag gcgatgcttt ccggcttctt gccgaagttt tcaatatgcg
     4921 cggcgacggt tttctggatc gcagtttctt tattcatgcg acgaccttat cgccggccgc
     4981 gagattgagc cataggagaa aaaggcaaag tcatggactt ttaagtcaaa acgcgcttaa
     5041 ttagtcagat gctggccacc ttaaatcata cgatcagcca aaaagttgcc gaggtgtttg
     5101 atctgtacac agagctgcac ggggcgcggg tttctttttt cggacccgac aagcagatgc
     5161 tgtatccgga cgaaaagggc cggccgaact gcgcctattg ccggatgctg cgcgaaacgc
     5221 tgaagctgga ttcccgctgc cgcgcgctgg accggcagat gatggatgcg gcgctgacga
     5281 agaacgaaat gatttcctac acctgccacg cgggaatgcg cgaagcggtt gcgccgctgg
     5341 ttgtaaacag cgagctggcc ggatttgtga tgatcggaca gttccgcagt aaagaagcgg
     5401 cggatgtttc tccgtatgcg gtccgatgga aaaaggagca ggggaacgat gcgttgcaga
     5461 cggcgttcgc ggacagcacc gtattcccgg aagagaaaat tgaaatactg ctcgccatgc
     5521 tgcggcaggt gctggaactg attatccgcg ggcagctgat tcaccgcaac gactacgatc
     5581 tgattgagcc ggtgattgcg cagatccgcc gccagccgga taaaaacatc actcttgaag
     5641 cggctgcgcg aataagcggg cgaagtccgt caacggtgac gcgcatgttt aaaaaggtga
     5701 cggggcagag tttcaagcag tatcaggtgc agttccgcct taagcgggcg gcggaactgc
     5761 tggtcgaccg gccgggccga ccggttgccg agattgccgc cgccgccggg tttgaagatc
     5821 cgttttactt ttcacggcag ttccataaac ataccgggct ttcgcccagc aactatcgta
     5881 acagcaaagc aggagaataa aatggccgca gacaaaacag cgttcgggaa aaatcaattc
     5941 ggcgtgaatg tggaactttt cactctgacg aatgaaaacg ggatccgtgt tcgcattatg
     6001 aaccacggcg gaacgattgt ttcgctcgaa gtgccggacc ggagcgggcg gctcgccgat
     6061 gtggtgctcg gccacgacac ggcggcggac tattgcgcgg cgacgccgta tttcggctgc
     6121 gcggtcggcc gctacgccaa ccgcatccag ggcggacgct tcacgctcga cggccagacc
     6181 tataccctcg cgcagaacaa cggcccgaac gcgctgcacg gcggagtgcg cggcttcgat
     6241 aaagtcgttt gggacgcaga aatcctgccg ggcgccgacg ccgtccggat gacctatatc
     6301 agcgccgacg gcgaagaagg cttccccggc gaagtcaaaa ccacgcttac ctatacgctg
     6361 accggcgcca acgaactgaa aatcgactgc atggcggaaa cggacaaagc cactccctac
     6421 aacatcacca accactccta cttcaacctg gcgggccacg acgccggaga gatcggcgcg
     6481 cagcggatga tgatccgggc cgaaaccctg ctgccgatcg attcgacctc gattccgctg
     6541 gggccggaaa tggcggtcgc gggcaccccg ttcgacttcc gcacgccgca cgccctgggc
     6601 gaacggatcg atgccgatca cgaacagatc cgccgcggat gcggctacga ccacaacttt
     6661 tgtctgacga aaacccggcc cggcgaactc agtctggcgg cccgcgccga ggatccggcg
     6721 agcggccggg tgatggaggt ttacaccacc gaaccgggcg ttcagctcta taccgccaac
     6781 tttctcgacg gacagctcgc gggcaagggc ggctgccggt atgtccgccg cggcgggttc
     6841 tgtctggaaa cgcagcacta tccggattcg cccaatcagc cgcagttccc gaataccata
     6901 ctgcgaccgg gaacggtgtt ccggtcgcag acggtctatc agtttaagtg agggcggcgc
     6961 gggccgcccg tcgaatgact agcggcgcgt ggtcggcggg aaatcgtcgg tgcggaacgg
     7021 cgaggccggc agcccttcgg cgttgaacag cgtcgggacg aaccacttct tccagccgta
     7081 gcgcacgaac ttggggctgg cgacgccggc ggcggagacc aggatcttgc cgtcggccag
     7141 ccgggcggcg gccggaacat aggtgccgtc ggcgccgcag atttcaaagg cgctgaccgc
     7201 ttccggtttc agcaccagtc cggagccggt gtctttgaag gagagttcaa tggcgccgct
     7261 tttggcttcg gcttttgcca gccgcgggcc gcggcagatc aggttggttt cgccgtagac
     7321 catttggcgg gccagcagcg agagccggta gcccactttg tccttcgagt gcgggtggat
     7381 gtttttgatg tcgccgccgt ccgtcagaac gatcatgccg ctgttttgca catggtcctc
     7441 ggtccagctc tgcgattcgc gcagttccgg ccaggcggcg gatttttcgg ttgcggggcc
     7501 gtaggcggcg agctggacga acaggaacgg caggttttcg ttccggaact cttcgcgcca
     7561 ttccttgatc agcgcaggaa atacggtgcg gtaatggaag ccgttcgcgg cgttgttttc
     7621 gccctgatac cagataacgc ctttcaccgt gtacggaatc gtctgtgaca gcatgttttc
     7681 atgcagaccg cacggccggt ttttgaagcg cgggccgatc ttcatgtccg gcttggggcc
     7741 gggcttttcg ccggcggctt gcttacgttc ccattctttg acggcggccg ggtaggcctc
     7801 cagatatttc acatagtcgg cttcgttggt gaagttggcc cggtagttct ggtcataggt
     7861 ctcaatcgtc cgtttcagat cgggatggtt ttccagcgtt ttgcggctca tccacgcttc
     7921 ggccggagtt ccgcccagcg agcagctgac gataccgacc ggtaccttca gcgtttcgcg
     7981 cagatttttg gcgaagtaat aggcgctggc cgagaagttt gcgacattgt ccggagtgct
     8041 ggccgcccat tgcggacggg cgctcggctg gctttcatac agcttttcgg caatcgtcac
     8101 catgcgcagc agcggatcgt tggcgctgtt gatgtccggc tgcgttgtcg gtttgaaacc
     8161 cttcatcgag tactgcatgt tggactgccc gccggccagc cagacttcgc cgacgaggat
     8221 gtctttcagc tccaccggcg ccgcggtttg cgagcctttg agaaccagcg tccggccttc
     8281 ggcgcaggcg ggcatcgctt tcagcgcgac catccagcgt ccggtctcgt ccgcgacggt
     8341 ttcttcggtt tggtcggcga agagaacggt gaccttttct ccggcggccg cggttcccca
     8401 gattcgcacc ggctcgccct gctgtaaaac agcgccgtcg gtaaacacgc ccgccggctg
     8461 aacagcggca aatgccgtaa aggcacagag tgaaacaagg aatacggtta tcaaacgacg
     8521 catggcagac cctttcgatt ggttaagccg cgcatcctgc cgcttccccg gcggaatgtc
     8581 cagagcctgt tttctgccgg atcttggaaa aaaaggttcc aatgtccggc gaaaccggct
     8641 cttatgtttt cagaaggttg aaacccatgg acgaaacaga aaacagtgaa atgcgcgccg
     8701 gagtggtggc ggtggtcggg cggacgaacg tcggcaaatc cacgctgctc aacgcgctgg
     8761 tcggcgaaaa agtcagcatc gtcagcgaca ccgtccagac gacgcgcaac atcatccgcg
     8821 ccattctaac cgagccgcgc ggccagatcg tcttcctcga cacccccggc atccacaaag
     8881 ccgagggcga cctcggcaaa aacctcaata aaatggcccg ctcgtcggtc aacggcgccg
     8941 atgccgtcct gctggtgctg gatgcgtcca gcaaaccgtg gcaggaggac gacggctgga
     9001 tgcgccgcct cgccgccgaa acaacgcctt gcgcaatcct gctcaacaaa accgacctcg
     9061 gcacatcctt tgttgaaacc tataaaaccc tttggaacga gatcgcgacg gagaagaact
     9121 cgaccgttca gcccgtctgg atcgaaggct cggcactgaa aggcggcgga ctgccggagc
     9181 tcgccgccgc gctgttcgcc ctggtgccgg aaggcccgcc gatgtttccg gaagacattc
     9241 tgaccgatta tccgcgcaag ctgaatatgg ccgatgtcat ccgcgaaaaa tatttcgacc
     9301 ggctgcacga cgaactgccg cactgcctgg ccatctgggt ggaaaccatc gaagaaactc
     9361 cggcggcgtg gtcgatccag gcggaagtcc tggtcgagcg ccattcgcaa aagggcatcg
     9421 tcatcggcga aaaaggccgc ctgctcaaaa gcgtgcagta ccagtccgaa aaagagctga
     9481 ccgaaatgta cggcctgccc gtcaaagtaa agctttgggt caaggtcgaa cccaactggc
     9541 gcaaaaactt ctggattctc aagaagcttg ggtatgcata aaaaaaccgc ccgtgaaatg
     9601 tccgggcggt tgttcgagcg gctgctgtta tttacctaac agcccaccga gtcctttttt
     9661 gacggagtcg accgcgcctt tcgcggcgtc cgtggcgcct ttggcggcat cggtggcggc
     9721 cccgcccgca tctttcagcg ctttgccggc ggcgtcgccg acggaagaag ccgcttcggt
     9781 cacggctttc gtgatggagt tgaatacttc ggaaatcact tcagccgggc tggcaccctg
     9841 cgattccttg ccgatatctt tcatgtgaat gtcgggcagc ggcagggtca gcttcttgcc
     9901 gcccagcgcg gtaaagctga cgttcacttt cgccccgttg agctggaagt cgttgataat
     9961 cactttcttc gcggctttct tttcggccgg tttggcggcc ggcgcggttt cggtcttcgg
    10021 cgcggcggtt tctttgggcg tcagattggc ctgcagggtg ctcaggttgc tggacttgag
    10081 gctcttttca taggtgatct gcggagcgtc gatcagaatc tgttcaatga cgatggcttt
    10141 ggagaagagc gacggcattt tgatctttaa tttgaaatcg ttcagttcca tggcgcttgg
    10201 cgtgttgaag ccttcggggt tgccgataac gagtccttta atctgtacca ggccggtgaa
    10261 ggggttaacg acaactttat cgagatgaac cgggacgccc gccagttttg gtccggcagt
    10321 gtttacacct gttttgatga tccggccgag agaaagcatc agcgcaacaa ccagaatcag
    10381 aagggatgct cctgtaatca atagaacctt tttcgttttt gtcgtcatac gccacccgtt
    10441 cctttctttt gttaaacgca gtttgaagtt ttgtttataa ccggacaagt ccgatctgaa
    10501 atcaaaatag aaaagccgcc ccggaaccgg cgcggctttt caatgcgggg agctcaggct
    10561 tacttcaggt ttttgtaata aacaacctca tcgttaaccg cgaagagctt tgcttcggtg
    10621 aatacatggt tttctgcgcg ggccaaatca catttaacgg cggcggtttc agctgcttct
    10681 tcgagggttg cgcggctgtg ttcgtagccg aacgcccact gtccggtcgg gtcgactgcc
    10741 accaccatta ctttctgacc gggcatttct ttgtatttga gaaaaatcgg gttaaatgct
    10801 tttggcggct caaggcgggt ttcaaacttc tgaccggatg aagcgcatcc gtaaacagat
    10861 gaaagcagaa cagacagaag gacaattttg atcagtgttt tcatgatttt ctcctatcag
    10921 gttgatggaa acacaggaaa ccgtaaaac
//
LOCUS       DAOPZO010000060        10864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_6950_length_10864_cov_8.905819, whole genome shotgun sequence.
ACCESSION   DAOPZO010000060 DAOPZO010000000
VERSION     DAOPZO010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 10864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10864
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6950_length_10864_cov_8.905819"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PKI68_05255"
     CDS             complement(<1..742)
                     /locus_tag="PKI68_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNX53131.1"
                     /translation="MVRKEGHTAKHLIQLQELVAARAQQQASMPSQRLQQLDKNIATL
                     AGELPVELKTHFNRLLQKSIEAIVPVTGGNCSGCGFALTKTMINSIHAGDELNRCPNC
                     TRILYAPDIPLTRRSPRRRWGDPVKRGIERFSAPELMIASLQGTTKEEVLLEMCENLR
                     AEGYIENCTDLHDAALRREAIVSTAVEHGIAFPHVRGVEGGGLTLTLGVSKKGVEFGA
                     PGEKLTKIFFYMVIPTAASAFYLKLLSGL"
     gene            complement(755..1363)
                     /gene="thrH"
                     /locus_tag="PKI68_05260"
     CDS             complement(755..1363)
                     /gene="thrH"
                     /locus_tag="PKI68_05260"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HNX53132.1"
                     /translation="MQIACLDLEGVLVPEIWINVAERTGIKELRVTTRDISDYDVLMR
                     RRLHILDEHSLKLADIQKVIAGMGPLPGAHEFLNRLREKFQVIILSDTFYDFAAPLMK
                     QLGHPTLLCHRLKIDAAGRVADYHLRMPDQKREAVKALHHLKFKVVAAGDSYNDTAML
                     AEADAGILFRPPQNVIDEFPQFQVTTTYNELYDAFLRAGTEI"
     gene            1532..3052
                     /locus_tag="PKI68_05265"
     CDS             1532..3052
                     /locus_tag="PKI68_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNX53133.1"
                     /translation="MPSFIEKIGKLFKKQETYRPSQSKASPKPDAETKKPAVKPQPKA
                     EAPRKHKTHRGGQRHLPGHTEKPVQPRKPAEPWDPATFQVEPKEGKTRFQDMNLPGEI
                     FHAIADLNFQYCTPIQAEILPLTLAGKDMAGKAQTGTGKTAAFLLGILTRFIKSRQEA
                     PKPGTPRALILAPTRELAMQIRDDAEKLSKYTPFRTVAIYGGIDYDKQRRMLEQPVDI
                     IVATPGRLIDFASQGVINLREVEVLVIDEADRMLDMGFIPDVRKIIYKTPPKENRQTL
                     LFSATLDDEVMRLASAWMTDPGKIEIEPDQNATKLIDQQVYIVTDDQKFALLYNILKK
                     EVGDSSALIFTNRRDQTERLADALFRYGIDCEILSGAVAQNKRQRILADFKDGRCKLV
                     VATDVAGRGIHVDDITHVINFNIPSQPDDYVHRIGRTGRAGKKGISVTFACEKESFEL
                     PAIEELLGKRLSCIQPEPDMLALPPPMRKSPFRESRPDSGRDSRRGPPPRRSGPRR"
     gene            complement(3054..4091)
                     /locus_tag="PKI68_05270"
     CDS             complement(3054..4091)
                     /locus_tag="PKI68_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53134.1"
                     /translation="MDFGPVGGGETPLSLGSRDIPGDLDIGVTGVNFFGTRSSGEKII
                     FILDASRQMMEDEKGGYYTYKYAKDKIHQLVDAMPAATLFNVMVYNDSNVDMFRPQPV
                     PATQANRDALKAWLAPINSDPHNVGQVASKYRSSVQYKSEIGGRARHWLKAVQAAMEQ
                     TADNIFVLCAGFGQYSAPSVRADGPPRKVERDENQMAEYREKVAAVNEKARKAFDAEN
                     AARAKKGLPPKIVYDWNDYMTQELRLVMPDPPPATGAENGGSGRTPTEPEKLVLDHMD
                     AVVACHYTPQKIMPPRVHFVYLVAADASLSAGYLDLMALEKTADAFRGEFELLRGAKT
                     MKNLIRYNPEF"
     gene            complement(4052..4459)
                     /locus_tag="PKI68_05275"
     CDS             complement(4052..4459)
                     /locus_tag="PKI68_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53135.1"
                     /translation="MQNPGVKIGIKKWPERFRGLKDPGRAMKRKITRCAIEAGVISVG
                     IHLLLFFFAGSVVVWNVVHKPAAVFKQENIEHPVLERRAAQLPVKIREQQRKGPPAGV
                     APRVVSAAALPFTLPSMSLPIWISGRSAAGKRR"
     gene            complement(4568..6565)
                     /gene="ftsH"
                     /locus_tag="PKI68_05280"
     CDS             complement(4568..6565)
                     /gene="ftsH"
                     /locus_tag="PKI68_05280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNX53136.1"
                     /translation="MENENDKPLPPEPEKGGAPRKKLPQVKPPMPMKGFAAWFLLMAL
                     VLMALQFFQKPEQTEQTSYNPDFVQMVKSGRIISCEIVRDTSGNDYIAGELTAVDGKT
                     GAPKKFRVDVVVTEDLLKMLQENNVTFKVRAHSPFWQVFWNVAPFIIGFLIIYFFFFR
                     QMKMAGGRAMSFGKSRAKLLQRDDKNRTTFADVAGVDEAKEELQEVVEFLKNPKQFQR
                     LGGKMPKGVLLAGSPGTGKTLIARAVAGEADVPFFSISGSDFVEMFVGVGASRVRDMF
                     EQGRKSAPCIIFIDEIDAVGRSRFSGMGGGHDEREQTLNALLVEMDGFDTTEGVIIIA
                     ATNRPDVLDPALMRPGRFDRQVFIDLPNLKGREEILNIHVKRVTLAKDVDLTKVARGT
                     PGFSGADLMNLVNEAALLAARRGAEAVDLPDFEEARDKVSWGRERRSHMLDAEEKKLT
                     AYHEAGHAIVLAKTEQTEPVHKVTVIPRGRALGATMQLPEKDRYTQGRKRLMGMLIGL
                     MGGRAAEELIFGDVTTGAQNDIERATRIARAMVCEFGMSETLGPRNYGSDQEQMFLGR
                     EISRSEKHSEQITLKIDEEIDSILKEAHKTATNILKEHRDRLELIAELLIKYETLDGE
                     QVMEILKTGKAPEELLKNGNGHAAAPVLSDEVKPAEVPPAE"
     assembly_gap    6603..6702
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6703..7613)
                     /gene="tilS"
                     /locus_tag="PKI68_05285"
     CDS             complement(<6703..7613)
                     /gene="tilS"
                     /locus_tag="PKI68_05285"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNX53137.1"
                     /translation="MNLIEKIKAVIEREGLIPAGATVIVGVSGGADSVALLHLLHRLG
                     VPIIAAHLNHSIRGAEADGDEAFVKTLCQKLGVECVAQKTDVPALAKEKGVSIEMAAR
                     QARHELFKSQVERRTSNVIVALAHHADDQLETFFLRAARGTGPGGLGGMRVVQELDGL
                     TLIRPLLGIRRAEIINWLREENIEWREDATNTDEAIPRNFVRHQLLPLFGKLNDRAAE
                     NLLRTMEILRDEEDHPGKAARRREIRDWLIERGVTPTFDAVEQVVAFSEKTNGTQFLD
                     LEGLRIVNEYGALKVGQAFSLPGQPERP"
     gene            7779..9413
                     /locus_tag="PKI68_05290"
     CDS             7779..9413
                     /locus_tag="PKI68_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNX53138.1"
                     /translation="MILRRSFLGVAGLSLASAQLVPAVPVAKEENQDRPPNIIILYTD
                     QQRYDMIAALGNNLIKTPNLDRLCREGVACTEAFATAPVCAPSRTSLFSGQYTTSHRT
                     YSNHHSGPRPNTNLALLLKQRGYKTGLFGKNHCFLAKGKDIDVMSGEVAPGLDAGNPE
                     AWFKAAGITINPPEKKPEVRQKGGADQPKISKRLTKSNKPANENPMHQVTDKALSFIG
                     SCQESQQPFFAWVSWLYPHTPYEASEPFYSIYASCNLPPVSLEPEGLEKANKPFRQIF
                     HQENNNRLLPFDANLTDRMRKTYMGAISEIDYEIGRILDYLDSRNMNENTILVFTADH
                     GDYQGDHGLYTKSPALYDCLIRVPLVFRWTKHWKDNVKSSELISQVDLMPTLLRAAGV
                     KVPDQCQGIELNSFLADGASPKPIRQAVFAEYGIPGAQPFNRERLEKAYPDYQTKPID
                     VKGNRVPWEGNPVSLAGRFRMIRTHQWKLIHEPGGTSELYNLASDPNELKNLFGVPEY
                     KGIQTELLEQLITWQKSLPGIEADQAKAPEATGDND"
     gene            9486..10844
                     /locus_tag="PKI68_05295"
     CDS             9486..10844
                     /locus_tag="PKI68_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNX53139.1"
                     /translation="MKTYNLLFALGLVAIRPVAHAAETKPQRPNILIILTDDQGYADL
                     GCFGNPQNKTPRMDQLAKEGTKFTSFYAQAICGPSRSALLTGRYPNLSKGFSMPATEI
                     TFAEKMRDVGYQTVCIGKWDVSDRQAILNRMPNAKGFDYYFGTLGANDSGKVAFHENN
                     EPAGDTKEMGELTHLYTQKAIDWLKTKRSPDKPFVLYLAHTMMHSIIDASAPFKGKSG
                     GSLYRDVVEEFDSETGRLLDQLDELGLRENTLVIYTTDNGPWNQKKYYEGKAKKYPAG
                     TIFWGESGPLRNGKGSPYEGGYRTPCIVRWPGKVPAGKVSDAVWATVDFMPTFAALAG
                     FEVPKDRVIDGVDQTDLLLGKTDKGRESFYYDRAGIRMGKWKFIAANAYFRIFCIEDD
                     RKNEDELYDLDADIGEKNNLAKQHPEIVARLKAEYQKLNSRSPALEGKQKKREEEASA
                     HD"
BASE COUNT         2432 a         3236 c         2876 g         2220 t
ORIGIN      
        1 tcagaccgga gagcagcttc agatagaagg cgctcgcggc ggtcggaatg accatataga
       61 agaaaatctt ggtgagcttt tcgccgggag cgccgaactc gacgcctttt ttgctgacgc
      121 ccagcgtcag cgtcagtccg ccgccttcaa caccgcgtac gtgcgggaac gcaatgccgt
      181 gttcgacggc ggttgaaaca atcgcttcgc ggcgcaatgc cgcgtcatgc agatcggtgc
      241 agttttcaat atacccttca gcgcgcaggt tttcgcacat ttcgagaagc acttcttctt
      301 tggttgtgcc ctgaagagaa gcaatcatca gttcgggagc cgaaaaccgt tcgatgccgc
      361 gtttgacggg gtcgccccag cggcggcgcg gtgagcggcg ggtgagcgga atatcgggcg
      421 cataaagaat gcgcgtgcag ttcgggcagc ggttgagttc gtcaccggca tgaatgctgt
      481 tgatcatggt tttagtcagc gcaaacccgc agccggaaca gttgccgccc gtaacaggaa
      541 cgatggcttc aatgcttttc tgcagaagac ggttgaagtg cgttttgagt tcaaccggaa
      601 gttctccggc gagagtggcg atatttttat cgagctgctg caggcgctgc gacggcatgg
      661 aggcctgctg ctgggcgcgg gcggcgacca gttcctgcaa ctgaatcagg tgttttgctg
      721 tgtgtccttc tttgcgaacc atgagatttc tcctttaaat ttctgtacct gctctgagga
      781 aggcatcgta cagctcattg taagtggtgg tgacttgaaa ctgcggaaat tcatcaatta
      841 cattctgcgg cgggcggaac aggatcccgg catccgcttc ggcaagcatg gcggtgtcgt
      901 tataggaatc gccggcggca acgactttga acttcaggtg atgcagcgct ttgaccgctt
      961 cgcgcttctg gtcgggcatg cgcaggtgat agtccgccac gcggccggcc gcgtcgattt
     1021 taagacggtg gcagagcagg gtcgggtggc ccagctgttt catcagcggc gcggcgaaat
     1081 catagaaggt gtcggaaagg atgatgactt gaaacttttc gcgcagccgg ttcaggaatt
     1141 cgtgcgcgcc gggaagcggg cccataccgg caatgacttt ctggatatcg gcaagcttca
     1201 ggctgtgttc atccagaatg tgcagacgcc ggcgcatcag tacatcatag tcggaaatat
     1261 cgcgggtcgt gacgcgcaat tccttaatac cggttcgttc ggcaacattg atccagattt
     1321 ccggaaccag cacaccttcc agatctaaac aggcaatctg catgaatttc ctttcagcct
     1381 tcgaaagcgc cccattttaa attccaattt atttatggca aaataatacg gaaacctttt
     1441 taattccacc ccttctcaaa gatttgccgg ttccggccgc attcccgcgc tccaatcggc
     1501 aatcacagac cagaaaccgg aaatatagtt catgccttcg tttatcgaaa aaatcggaaa
     1561 actgttcaag aaacaggaaa cttaccgccc ctcgcagtct aaagcttccc ccaaaccgga
     1621 cgccgaaaca aaaaaaccgg ctgtaaaacc gcaacctaaa gcggaagccc cccgtaaaca
     1681 taagacccat cgcggcggac aaagacacct tcccggacac accgagaaac ctgtccagcc
     1741 ccgcaaaccg gctgagccct gggatcccgc cacctttcag gtcgaaccga aagaaggcaa
     1801 aacccgtttt caggacatga acctgccggg cgagattttc cacgccattg ccgacctcaa
     1861 cttccagtac tgcacgccca ttcaggccga aatccttccg ctcaccctcg cgggtaaaga
     1921 tatggccggc aaagcgcaga ccggaaccgg caaaaccgcc gccttcctgc tcggcatcct
     1981 cacccgcttt atcaaaagca gacaggaagc gcccaagccc ggaacgcccc gtgcgctcat
     2041 tctggcgccg acgcgcgaac tcgccatgca aatccgcgac gatgccgaaa agctcagcaa
     2101 atacacccct ttccgcacgg tcgccatcta cggcggcatc gactacgaca agcagcgccg
     2161 catgctggaa cagcccgttg atatcatcgt cgccacaccc ggacgcctga tcgacttcgc
     2221 cagtcagggc gtcatcaacc tccgcgaagt cgaagttctg gtcattgacg aagccgaccg
     2281 catgctcgac atgggcttta ttcccgacgt ccgcaaaatc atctacaaaa ccccgcccaa
     2341 agaaaaccgg cagactctgc tcttcagcgc caccctcgac gacgaagtca tgcgcctcgc
     2401 ctccgcctgg atgaccgatc ccggcaaaat cgaaatcgaa ccggatcaaa acgccaccaa
     2461 gctgatcgac cagcaggtct acatcgtcac cgacgaccag aaattcgccc tgctctacaa
     2521 catcctcaaa aaagaagtcg gcgacagctc ggcgctcatc ttcaccaacc gtcgcgacca
     2581 aaccgaacgc cttgccgacg ctctcttccg ctacggcatt gactgcgaga ttctctccgg
     2641 ggccgtcgcc cagaacaaac gccagcgcat tctggccgac tttaaagacg gccgatgcaa
     2701 actcgtcgtc gccaccgacg tcgccggacg cggcatccat gtcgacgaca tcacccacgt
     2761 catcaatttc aacatccctt cacagccgga tgactacgtt caccgcatcg gccgcaccgg
     2821 ccgcgccggt aaaaaaggaa tatcggtcac ctttgcctgc gaaaaagaat cgttcgaact
     2881 gccggccatt gaggaactgc tgggcaaacg gttgtcctgc atccagcccg aaccggacat
     2941 gctggcactg cccccgccga tgcgcaaatc gcctttccgt gaaagccgcc cggacagcgg
     3001 aagggacagc cgccgcggtc cgccgccgcg ccgcagcggc cctcgccgtt aaatcaaaat
     3061 tcgggattgt accggatcag atttttcatg gtttttgcgc cgcgcagaag ttcaaactcg
     3121 ccccggaaag catccgctgt cttctccagc gccatgagat ccagataacc cgcagacaaa
     3181 ctggcgtccg ctgcaaccag gtagacgaaa tgcacccgtg gcggcattat tttttgcgga
     3241 gtatagtggc aagctacaac cgcgtccatg tgatccaaaa ccagcttctc cggctccgtc
     3301 ggcgtgcggc cggagccgcc gttttccgca ccggtcgcgg gaggcggatc cggcatcaca
     3361 agccgcagtt cctgcgtcat gtaatcattc caatcgtaaa caattttagg cggaagcccc
     3421 ttctttgcgc gcgccgcatt ttccgcatcg aaagccttcc gggccttttc attcaccgcc
     3481 gccacttttt cacggtattc ggccatttga ttttcatctc tttcgacttt gcgaggcgga
     3541 ccgtctgccc ggaccgaagg cgcgctatac tggccgaacc cggcgcacag cacaaaaata
     3601 ttatcagccg tctgctccat cgccgcctgt accgccttca gccagtgccg ggctctgccg
     3661 ccgatctcgg atttatactg aaccgatgaa cggtatttcg aggcaacctg tccgacgtta
     3721 tgcggatcgc tgttaatcgg cgccagccag gctttcagcg catcccggtt ggcctgagtg
     3781 gccggtacag gctgcggccg gaacatatcg acgttcgaat cgttatacac cattacattg
     3841 aacagtgtgg ccgccggcat tgcatccacc agctgatgaa ttttatcctt ggcatattta
     3901 taggtgtaat aaccgccttt ttcgtcctcc atcatctgcc gggacgcgtc cagaatgaat
     3961 attattttct cgccgctgga acgggttccg aaaaaattta cgccggtaac cccgatatca
     4021 agatcaccgg gtatatcgcg gctgcccaga ctcagcggcg tttccccgcc gccgaccggc
     4081 ccgaaatcca tatcggcaga ctcattgacg gcagcgtaaa aggcagcgcg gcagcgctca
     4141 caacgcgggg cgcaactccg gccggcgggc cttttctctg ctgctcccga atctttaccg
     4201 gcagctgtgc cgcccgtcgt tcgagtacgg ggtgctctat attttcctgc ttgaaaacgg
     4261 cggccggctt atggaccaca ttccagacca ccacagaacc ggcaaagaaa aacagcagca
     4321 ggtgaattcc gacactgatc acgccggcct ctatcgcaca acgcgtgatt ttccgtttca
     4381 tcgcccggcc cggatctttc agcccgcgga atcgctccgg ccatttttta atcccgattt
     4441 tcacaccggg attttgcatt tcagaacagc agaaaaccag tttgttatct gagataaaaa
     4501 aagccccgct tccaaacggc ttggaaagcg gggcctgatc gttcgttccg gatcttccga
     4561 aggaacatta ttcagccggc ggaacttctg ccggcttcac ttcatcggac aaaaccggcg
     4621 cggcggcgtg cccgttgccg tttttgagaa gctcttccgg agctttaccg gttttcagga
     4681 tttccatcac ctgttcgccg tcgagcgttt catacttgat cagcagttca gcgatcagct
     4741 caagccggtc gcggtgttct ttcagaatat tcgtagcggt tttatgagcc tctttgagaa
     4801 tgctgtcgat ctcttcatcg atcttcagcg tgatctgctc gctgtgcttc tcactacggc
     4861 tgatctcgcg gccaagaaac atctgctcct gatcggaacc gtagttgcgc gggccgagcg
     4921 tttcgctcat gccgaactcg cagaccatcg cgcgggcgat gcgggtggca cgctcgatat
     4981 cgttctgcgc accggtagta acgtcgccga aaatcagttc ttcggcggcg cggccgccca
     5041 tcaggccgat cagcataccc atcagacgct tgcggccttg cgtgtagcgg tctttttccg
     5101 gcagctgcat ggtggcgccc agcgcgcggc cgcgcggaat aaccgtcact ttatgaaccg
     5161 gctcggtctg ctcggttttc gccaatacaa tggcgtggcc cgcttcgtgg taagccgtca
     5221 gctttttctc ttcggcgtcg agcatatggc tgcggcgttc gcgaccccag gacactttgt
     5281 cgcgggcttc ttcaaagtcg ggaagatcta cggcttccgc accgcgccgc gccgccagca
     5341 gcgccgcctc attgacgaga ttcatcaggt ccgcgccgga aaaaccgggc gtaccgcgcg
     5401 ccactttggt gagatccaca tctttagcca gcgtaacgcg cttaacgtga atgttgagaa
     5461 tttcttcgcg ccctttgaga ttcggcaggt cgatgaaaac ctgacggtca aaacggcccg
     5521 gacgcatcag cgccggatca agcacgtcgg gacggttggt ggcggcgatg ataatgacgc
     5581 cttcggtcgt atcgaacccg tccatttcga ccagcagcgc gttcagtgtc tgctcgcgtt
     5641 cgtcgtgtcc gccgcccata ccggaaaacc ggctgcggcc cacggcgtcg atttcgtcga
     5701 tgaaaataat acacggcgcg ctcttgcggc cctgctcaaa catatcgcgc acgcggctgg
     5761 cgccgacgcc gacaaacatt tcgacaaagt ccgaaccgct gatggagaag aacggcacgt
     5821 cagcctcgcc ggcgacagct ctggcgatca gcgttttacc ggtaccgggc gaaccggcca
     5881 gcagaacgcc tttcggcatt ttgccgccga gccgctggaa ctgtttcggg tttttcagga
     5941 actcgaccac ttcctgcagc tcttccttcg cctcatccac gccggcaaca tcggcaaaag
     6001 tggtacggtt tttatcgtcg cgctgcagca gtttggcgcg gctcttgccg aagctcatcg
     6061 cgcggccgcc ggccatcttc atctgccgga aaaagaaaaa gtagatgatc aggaagccga
     6121 taatgaacgg cgccacgttc cagaatacct gccagaacgg cgaatgcgcg cgcaccttaa
     6181 aggtcacgtt gttttcctga agcattttca gcaggtcttc agtgaccacc acatctacgc
     6241 ggaacttctt gggcgcgccg gtcttgccgt ccaccgccgt cagttcgccg gcgatgtagt
     6301 cattgccgga tgtatcgcgc acgatttcgc agctgataat tcggccggac ttcaccatct
     6361 ggacaaagtc ggggttataa ctcgtctgct ccgtctgttc cggcttctgg aagaactgta
     6421 aagccatcag caccagcgcc atcagcagaa accaggcggc gaagcccttc atcggcatcg
     6481 gcggtttcac ctgcggcagt tttttacggg gcgccccgcc cttttcgggc tccggcggca
     6541 gcggtttatc gttttcgttt tccataatta aatccttatt ggcgaagacc ctaacaccgg
     6601 ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggcctttc aggctgcccg
     6721 ggcaggctga aagcctgccc cactttaaga gccccgtatt cattgacgat gcggagacct
     6781 tcgagatcga ggaactgcgt tccgtttgtt ttttcagaaa atgcaacgac ctgttccacc
     6841 gcgtcaaaag tcggagtaac gccccgctca atcagccagt cgcggatttc acggcggcgg
     6901 gcggccttgc cggggtgatc ttcttcgtcg cgcagaattt ccatcgtgcg caaaagattc
     6961 tccgccgcgc ggtcgttaag ttttccaaac agcggaagca gttgatgccg gacaaagttg
     7021 cgcggaatgg cttcgtccgt attggtggcg tcttcccgcc attcgatatt ctcttcccgc
     7081 aaccagttga taatttccgc ccgccggatt ccaagcaacg gccggatcag cgtcagtccg
     7141 tccagttctt ggacaacccg cattccgccg aggccgccgg ggccggtgcc gcgcgccgcg
     7201 cgcaggaaaa aggtttcgag ctggtcatcg gcatgatgcg ccagtgcgac tatgacgttc
     7261 gacgttcgac gttcgacctg cgacttaaag agctcgtggc gggcctgccg ggcggccatt
     7321 tcgatggaaa cacctttttc tttggcgagc gcgggaacgt cggttttttg cgcgacgcac
     7381 tcgacgccga gtttttggca gagcgttttc acgaacgctt cgtcgccgtc ggcttctgca
     7441 ccgcgaatgg aatggttcag gtgcgcagcg atgatcggga caccgagccg gtgcagaaga
     7501 tgcagcagag cgaccgagtc ggctccgccg gaaacgccga cgatgaccgt cgcgcccgcc
     7561 ggaatcagcc cttcgcgctc aatcaccgct tttatttttt cgatcaggtt cacagcggct
     7621 aattaacgcc aatggccgcg aattaacaag accggaccaa tagaaggtcg ccgcaccgct
     7681 caaaggtatt tttgcgttcc aaaaaacaac agctttgatt acactagatc gttacaatga
     7741 gcgtttgttg aaaacgatcg accaatagga gcagcaggat gatattacga cgcagtttct
     7801 tgggtgtggc gggtctgagt ctggcttcag cccagctggt tccagcggtc cccgttgcga
     7861 aggaagagaa tcaagatcgg ccgcccaata tcatcattct ttataccgac cagcaacggt
     7921 atgacatgat cgccgccttg gggaacaacc tgatcaagac gccgaatctg gatcgtctgt
     7981 gccgggaagg tgtggcctgc acagaggcat tcgccacggc gccggtgtgt gcgccatcac
     8041 gcacatctct tttctccgga cagtatacga catcacaccg aacttattcg aatcatcaca
     8101 gcggaccgcg cccgaacacc aatctggccc tcttgctgaa acaacgcggc tacaagaccg
     8161 gactcttcgg aaagaaccat tgctttctag ccaagggcaa agatatcgac gtcatgtcag
     8221 gggaggttgc acccggcctg gacgcaggca atccggaggc ctggttcaaa gcggccggaa
     8281 taaccatcaa cccgcccgag aagaagccgg aggttcggca gaaaggcggg gccgatcagc
     8341 cgaaaatatc caagcggctg accaaatcga ataagccggc caatgagaat ccgatgcatc
     8401 aggtgaccga taaggcgctc tccttcatcg gctcctgcca ggaatcgcag caaccgttct
     8461 tcgcatgggt ctcctggctg taccctcata ccccctatga agcgagcgag cctttctaca
     8521 gcatctacgc gagttgcaat ctgccgcccg taagcctgga accggaaggt ctggaaaaag
     8581 ccaacaagcc ctttcggcag atcttccatc aggagaacaa caaccggctg cttccgtttg
     8641 acgcgaacct taccgacaga atgcgcaaga cctatatggg cgccatctca gagattgact
     8701 atgagattgg ccgcatcctt gactacctcg acagccggaa tatgaatgag aacaccatcc
     8761 ttgtcttcac cgccgatcac ggggactacc aaggcgacca tggtctctac acgaaaagtc
     8821 ctgcgctgta tgattgcctg attcgcgtac cccttgtctt ccggtggacg aaacactgga
     8881 aagacaacgt gaagagttcc gaactcattt ctcaggtgga tcttatgccg accctgctcc
     8941 gggcggcagg ggtaaaggtg ccggaccagt gtcaggggat cgaactgaac agcttcctgg
     9001 ccgacggcgc ctctccgaaa ccgattcgcc aggctgtgtt tgcagaatac gggattcctg
     9061 gagcccaacc cttcaatcgt gaacgcctgg agaaagcgta tccggactat caaaccaaac
     9121 cgattgacgt gaaaggcaac cgggtccctt gggaaggcaa cccggtttcg ctggccggtc
     9181 gtttccgcat gatccgaacc caccaatgga aactcattca tgagccgggc gggacgagcg
     9241 aactctacaa tctggcgagc gatccaaacg aactgaagaa cctgtttgga gtccctgaat
     9301 acaaagggat tcaaaccgaa cttcttgagc agctcatcac gtggcagaag agcctgccag
     9361 gaattgaagc cgaccaggcg aaggcgcccg aggcaacggg tgacaatgat tagggccccc
     9421 atccgttgaa ttgataaacg aaaaggttgg aaaatctgaa acttaacctc taaacacctc
     9481 atactatgaa aacatataac ttattattcg cgttgggact tgtggccatc agaccggttg
     9541 cgcacgccgc agagacgaaa ccccagaggc cgaacatcct catcattctc accgacgatc
     9601 aagggtatgc ggatctgggc tgttttggaa acccccagaa caaaacgccg cgcatggatc
     9661 agctggcaaa agagggcaca aagttcacca gcttctatgc gcaagccatt tgcgggcctt
     9721 cccgttcggc gctgctgacc gggcgctacc caaacttgag caaagggttc agcatgccgg
     9781 ccacggaaat caccttcgcc gaaaagatgc gcgacgtcgg ctaccagacc gtttgcatcg
     9841 gcaagtggga tgtttccgac cgccaggcca ttctcaaccg catgccgaat gccaagggct
     9901 tcgactatta cttcggcacg ctgggcgcga acgactcagg caaagttgct tttcacgaaa
     9961 acaacgaacc ggccggcgat acgaaggaga tgggcgaact gacccacctc tacactcaaa
    10021 aagcgatcga ttggctgaaa accaagcgct cgccggacaa gccgttcgtc ctctacctcg
    10081 cccacaccat gatgcactcc atcattgatg cctccgcgcc gttcaaaggc aaatccggcg
    10141 ggagccttta ccgggacgtg gtggaggagt tcgacagcga aaccggacgc ctgctcgacc
    10201 aactggacga actcgggctt cgcgaaaaca cgctcgtgat ttacaccacc gacaacggtc
    10261 cgtggaatca gaagaagtat tacgaaggaa aggcgaaaaa atatcccgcg ggcaccatct
    10321 tctggggcga atccgggccg ctgcgcaacg gcaagggctc gccgtatgaa gggggctacc
    10381 ggaccccgtg catcgtgcgc tggcccggaa aagtgccggc cggcaaggtg agcgacgccg
    10441 tctgggcgac cgtggatttc atgccgacgt ttgcagctct cgccgggttt gaagtgccca
    10501 aggatcgcgt catcgatggc gttgaccaaa ccgatttgct cctggggaaa accgacaagg
    10561 gccgcgagag cttctactac gaccgggccg gcatccgcat gggcaaatgg aaattcatcg
    10621 cggccaatgc gtatttccgc atcttctgca tagaggacga tcgaaagaat gaggatgaac
    10681 tctatgattt ggatgcggat atcggcgaaa agaacaacct cgccaaacag catccggaga
    10741 tcgtggcccg tctgaaagcg gaatatcaga aactgaattc gcgttctccg gccttggagg
    10801 gtaagcaaaa aaaacgcgag gaagaagcca gcgcccatga ttagaacccg gtgcgccgca
    10861 ccgt
//
LOCUS       DAOPZO010000061        10725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_7091_length_10725_cov_8.069822, whole genome shotgun sequence.
ACCESSION   DAOPZO010000061 DAOPZO010000000
VERSION     DAOPZO010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 10725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10725
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7091_length_10725_cov_8.069822"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="PKI68_05300"
     CDS             <1..905
                     /locus_tag="PKI68_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNX53140.1"
                     /translation="LVNCARAGVVNENDLRAIKAEKGLRFLNDVYAKDAEGPKSVIDI
                     ADIMVPHLGASTKEANFNAAQRSAEQLIDFDSKGVTSYIVNRDIPEGLDEAYADLTYT
                     IAALARAMIGAETKLSRIEASFYGDLRRFAKWLTVPVVCALSKEFDRSMDPKAAVAFL
                     KDMGIEYSTRETDETKGYGNSITIDLMASIDKDSLRRISVRGTVAEGLMMISRIGGFD
                     KLYFEPKGHIVLFTYMDRPGVLSEIAGAMAKEGINIDDVRNPHDKTGERSIAILKVSQ
                     PVPEKVLAKVAGKIEAISFCTVSF"
     gene            921..1073
                     /locus_tag="PKI68_05305"
     CDS             921..1073
                     /locus_tag="PKI68_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53141.1"
                     /translation="MFKKLFYLTVVLLLCSSCVSRTTIKEPGLKDAGEKSRSETKLIW
                     FWDKDF"
     gene            1120..2814
                     /locus_tag="PKI68_05310"
     CDS             1120..2814
                     /locus_tag="PKI68_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNX53142.1"
                     /translation="MAPKKEFSILLAGEAGQGINSIEAILVRLLKQSGFHVFAAKEYM
                     SRVRGGTNSTSIRISGRRVSAPVDTVDLFIPLTEAAVQRYQHRFSDNTITVGEQAVVK
                     SDRMHDVPFASIGKELGDKRYASMVAAGVVAGMLDLDPDVLSALIQKRFAGKDSAVTE
                     ANIKAAAHGIQAGKEIAQKIHLSVAIPQSGETASELMMTGTDAVALGAVAGGCDSVFA
                     YPMTPGSGVFTALAQTSHETGILVEQVEDEIGVINMALGAWYAGARALVSTSGGGFAL
                     MTEGISLSGMTETPVVVHVGQRPGPATGLPTRSEQGDLNLVLHAGHGTFPRIILAPGS
                     LEQAFFLTQKAFNLADEYQIPVFILTDQYFVDMYYNLPAFDLTGLENKNGFVEAGEDY
                     KRYRLTDSGVSPRGIPGFGPGRVCADSDEHDESGRITEDLDGISRQMKDKRFRKFDLV
                     KQAAVAPDLYGPEDYKMLFVAWGSTLPAAREALERTGSKTAALLHFSQVWPLHSGTLR
                     LIEKAERIIAIENNQTGAFSDLLRMETGAAVERKILKYNGLPFSVEELAARMKEVL"
     gene            2814..3668
                     /locus_tag="PKI68_05315"
     CDS             2814..3668
                     /locus_tag="PKI68_05315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNX53143.1"
                     /translation="MNTDYFDIPNVEKAWCPGCGNFGLRAIVMNAYAELGLKKENLVM
                     VSGIGQAAKSPHYYDVSYFNGLHGRALPAATGIKAANPELTVVVQSGDGDMYGEGGNH
                     FLHTIRRNPDITVIVHDNMVYGLTKGQAAPTSQIGMKTPVQIDGVFEEPINPLTLAIS
                     LGATFVAQGSAGERELTTELIKQGIRHKGFALINVFQACPSFNKLNTAQWFKDNIRAV
                     PDDYDPSDKAAAFRKALETVPWPVGVIYRETGKPTFEDHIHKNDSTPLYRRPRSTERV
                     KKLLESKR"
     gene            complement(3670..4173)
                     /locus_tag="PKI68_05320"
     CDS             complement(3670..4173)
                     /locus_tag="PKI68_05320"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNX53144.1"
                     /translation="MSDRLQQRLAEIGAPADVLELAAELAEQAVRDPLTGLYNRRFFD
                     EALAQQVEAARRYGRGLSLVLLDLDDFKAVNDTRGHEAGDAVLRGFAQTLRAAARKSD
                     IVCRIGGDEFAVILPETGAASARKFTERFFLMAEPLAGVSAGIAALPSENLFAEADAE
                     LLRTKQA"
     gene            complement(4166..4813)
                     /locus_tag="PKI68_05325"
     CDS             complement(4166..4813)
                     /locus_tag="PKI68_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HNX53145.1"
                     /translation="MKLVCKKEVWMPTWQGFVSGILLVGALVCGAVCHLYPFLAQNHP
                     QPAAGIIIIEGWMADAELKAAAGAIQPGQIVVTTGGPVTFGQKILQYDNYAELGTARL
                     VEYGIPASSIITIPAPDTQRDRTYVSAVAARAKLEELGLFGKSANLYTVGAHARRSYL
                     LFRHVFGRNYPLGVIAVEPPSYNLSHWYRHSAGFKHVVTEFISWIYAQFFLLTHV"
     gene            4948..5382
                     /gene="rplM"
                     /locus_tag="PKI68_05330"
     CDS             4948..5382
                     /gene="rplM"
                     /locus_tag="PKI68_05330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNX53146.1"
                     /translation="MKTFVANENDIEREWFVVDATDKSTGRLAVFIADLLRGRNKPTY
                     TPHVDTGAFVVVINAEKVKLTGNKEENKIYQDFSGYPSGQQNRSAAVIREKNPTRIIT
                     QAVEGMLPGNRLMRRTITRLKVYTGPNHPHSAQKVTTLEFKG"
     gene            5423..5800
                     /gene="rpsI"
                     /locus_tag="PKI68_05335"
     CDS             5423..5800
                     /gene="rpsI"
                     /locus_tag="PKI68_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004695095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNX53147.1"
                     /translation="MAATGRRKTSVARVSLVKGSGVITVNGKTAKDYFDTKDMLDTVV
                     RPLVLTGRSDQYDMTVTVKGGGKVGQCGAISHGASRALVKAEEELKPILKKGGFLTRD
                     ARMKERKKPGQPGARKRFQFSKR"
     gene            5909..7090
                     /gene="argJ"
                     /locus_tag="PKI68_05340"
     CDS             5909..7090
                     /gene="argJ"
                     /locus_tag="PKI68_05340"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNX53148.1"
                     /translation="MKKTLSLPKGFSAAGIAAGIKKKKKDMALIVSDVMAESAAVFTT
                     NQVKAAPVKWDIKVVKHDGARAIVMNSGNANACTGAQGMADVEATAALAAGKLGINSL
                     DVFVCSTGTIGKPLPMDKIAAGIETLFDEVSPDGGMDAAEAMMTTDLVSKTVVAEIDL
                     DGVPVRITGLAKGSGMIEPNMATMLAFILTDAAVEKHTLQCALRTAADMSFNRITVDG
                     DRSTNDTVICLANGRAGNETITQRSTHWPLFLQTLEKVLFELSMMIVKDGEGATRVIT
                     VKVNGAASNGEAEEAARAVANSMLNKTAWAGKRPNWGRIMDAIGYSYAQVQENKVDID
                     YDDVPAVRGGMAAGTPEEELVAAVSKEAFTINIDLNLGTGHTVVYTCNCTEDYVRINV
                     E"
     gene            7105..7977
                     /gene="argB"
                     /locus_tag="PKI68_05345"
     CDS             7105..7977
                     /gene="argB"
                     /locus_tag="PKI68_05345"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNX53149.1"
                     /translation="MQQMIEKAAVLIEALPYIQKFRGDTVVVKFGGSIMESEIGYRNI
                     LKDVAFMECVGLQPVIVHGGGKSVSKKMREAHIQPNFVQGLRVTDAATIEVVEKVLNN
                     EVNPHLVEILKNYGGKARGIHGEDIITVKKHTGTDPQTGEELDWGYVGKVIHVDVEPI
                     KAFLNADIIPVITPLGRGEDGHLYNVNADDVANAIAAALQARKLVFLSDVPGLLRDVN
                     DPASLISTLSLAEVEDLIKRGIISGGMLPKIGGAVDALKAGVNKTHIIDSALPHSLLL
                     ELFTDKGVGTEIVK"
     gene            8318..9292
                     /locus_tag="PKI68_05350"
     CDS             8318..9292
                     /locus_tag="PKI68_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53150.1"
                     /translation="MPISKELRRTYHIDPFYTKTITSAGIAIVGSDKVSDWAFLEAAY
                     TMDHVLYESPAWVREALAATKVRMAILACVEYTMDLPENNRTPRTLAEAAFQDQRSRG
                     LGGLPDCSCAEENLLNLRGDPYDGDGSTDAGESITIHEFSHTLAGTIRKYQGLNGDFW
                     TKLNKAFADATAPDGYLGIQNKGRKVKIYASQSAQEYWAEGAQAWFDRADRRSSGGLH
                     TRDDVKRQDPQLAALLAEIYGDGPWRYHKTTAKKPDGTPMRTPDELAHLEGLDDLRKQ
                     FPAYNKNNSPRIMALMQETAARTAEEAGRNAVTNAPAPSTPESRPSAR"
     gene            9529..9996
                     /locus_tag="PKI68_05355"
     CDS             9529..9996
                     /locus_tag="PKI68_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53151.1"
                     /translation="MKKILALRIIETIFLFGIVFIKTITPEQWSKSTSYLAAGFHLNL
                     STNADTYIFSTLLIGYVVSLIASFMQRGIKNECLKPSFVFAILGVAGFSNEILRYIIE
                     YRFQLILSFPILLLIFDWLAFFRLLKKSPNQNIEPTLKTPVESGEVESGEAHV"
     gene            10023..10424
                     /locus_tag="PKI68_05360"
     CDS             10023..10424
                     /locus_tag="PKI68_05360"
                     /inference="COORDINATES: protein motif:HMM:NF026933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm51 family immunity protein"
                     /protein_id="HNX53152.1"
                     /translation="MPSWSIPSNLEEIIDSDDGGIWDDAETFAPMTLSVMKGTEYDGR
                     EIPHAWQIEFEPESEECEITNERISSMGLEADGYGWATLIDSVVRKYHPEIADELNFG
                     DTDTASLVVWVESEATCRKVAEVAWNLIFAT"
BASE COUNT         2619 a         3014 c         2954 g         2138 t
ORIGIN      
        1 cgctcgtcaa ctgcgcccgc gcgggcgtcg tcaacgaaaa cgatctgcgc gccatcaagg
       61 cggaaaaagg tctgcgcttc ctgaacgatg tttatgccaa agatgccgaa ggcccgaaga
      121 gcgtcattga tatcgccgac atcatggtgc cgcacctcgg cgcttccacc aaagaagcca
      181 acttcaacgc ggcacagcgt tcggctgaac agctcatcga cttcgacagc aaaggcgtca
      241 cctcctacat cgtcaaccgc gacattccgg aaggactgga tgaagcctat gccgacctca
      301 cctacaccat cgccgcgctg gcccgcgcca tgatcggtgc cgaaaccaag ctcagccgca
      361 ttgaagccag cttctacggc gatctgcggc gtttcgccaa atggctgacc gttccggttg
      421 tctgcgctct cagcaaagag tttgaccgct cgatggatcc gaaagccgcc gtcgctttcc
      481 ttaaggatat gggcattgag tattccaccc gcgaaacgga tgaaaccaaa gggtacggaa
      541 actccatcac catcgacctg atggccagca tcgacaaaga cagcctgcgc cgcatcagcg
      601 tacgcggcac agtcgccgaa ggtctgatga tgatttcgcg catcggcggt ttcgacaaac
      661 tctactttga accgaaaggc cacattgtac tcttcaccta tatggatcgc cccggcgttc
      721 tcagcgaaat cgccggcgcc atggccaaag aaggcatcaa cattgacgat gtccgcaatc
      781 cgcacgacaa gaccggcgaa cggtcgattg cgattctgaa ggtcagccag ccggttccgg
      841 aaaaggttct cgccaaagtc gccgggaaga tcgaggcgat cagcttctgc acagtttctt
      901 tttaacagaa aggtctcact atgtttaaaa agctgtttta tctgacggtt gttttactgc
      961 tttgcagctc ctgcgtcagc cggacaacta ttaaagagcc cggactgaaa gatgcagggg
     1021 aaaaaagtcg cagcgaaaca aaactgatct ggttctggga taaagatttt taaccggtac
     1081 tgacagatcc cgctccagaa cttaaaagga gaggtgttca tggcgccgaa gaaagaattt
     1141 tctattctgc tggcgggtga agccggacaa ggcatcaatt cgattgaagc cattctcgtc
     1201 cggctgttga aacagagcgg attccatgtt ttcgcggcca aggagtatat gtcgcgtgtg
     1261 cgcggcggaa ccaactccac ctcaatccgc atctccggcc ggcgggtgtc cgccccggtc
     1321 gataccgttg atctgtttat cccgctaacc gaagccgcgg tgcagcgcta tcagcaccgg
     1381 ttttcggaca acaccattac ggtcggcgaa caggccgttg tcaaaagcga ccggatgcac
     1441 gatgtgccct tcgcatccat cggcaaagag ctgggcgaca aacgttacgc cagcatggtc
     1501 gcggcaggcg ttgtcgccgg aatgctcgat ctcgatcccg acgtactgtc ggcgctgatc
     1561 cagaagcggt ttgccggaaa agacagcgcc gtcacagagg ccaacataaa ggcggcggct
     1621 cacggcatac aggccggtaa agagatcgcg cagaaaatac atctttccgt cgccatcccg
     1681 cagtccggag aaaccgccag cgaactgatg atgaccggaa ccgatgccgt cgcgctcggc
     1741 gccgtcgcag gaggctgcga ttcagtcttt gcttatccga tgacgcccgg ctccggcgtc
     1801 ttcaccgcgc tggcacagac ctcgcacgag acaggcattc tggtcgaaca ggtcgaagac
     1861 gaaatcggcg tcatcaacat ggcgctgggc gcgtggtatg ccggtgcgcg ggcgctggtg
     1921 tccacctccg gcggaggatt tgcgctgatg accgaaggaa tcagcctgtc cggcatgacc
     1981 gaaacgccgg tggtggtaca tgtcggacag cgccccggtc cggcgaccgg actgccgacc
     2041 cgctccgaac agggcgatct gaatctggtt ctgcacgccg ggcacggaac gtttccgcgg
     2101 atcatcctgg cacccggttc actggagcag gcttttttcc tgacccagaa agcattcaat
     2161 ctggccgacg aatatcagat tccggttttc attctgaccg accagtattt tgtggatatg
     2221 tattacaatc tgccggcatt cgacctgacc ggcctcgaaa acaaaaacgg cttcgtcgag
     2281 gccggcgaag attataagcg gtaccggctg acagacagcg gcgtctcgcc gcgcggcata
     2341 cccggattcg ggccgggccg ggtctgtgca gattccgacg agcacgatga atcgggccgt
     2401 atcaccgaag acctcgacgg catcagccgg cagatgaaag ataagcggtt cagaaagttc
     2461 gacctcgtca aacaggccgc ggttgcgccg gatctctacg ggccggagga ttacaaaatg
     2521 ctcttcgtcg cctggggctc caccctgccg gcggcccgcg aagcgctcga acgcaccggt
     2581 tctaaaacag ccgctctgct ccacttctca caggtctggc cgctgcactc cggcacactg
     2641 cggctcatcg aaaaggcaga gcggattatc gccatcgaaa acaaccagac gggggcgttt
     2701 tccgatctgc tccgcatgga aaccggtgcg gcggtcgaac ggaaaattct gaaatataac
     2761 gggctgcctt tctccgttga ggaactggcc gcccggatga aagaggttct ctgatgaaca
     2821 ccgattattt tgacatcccg aatgttgaaa aagcctggtg ccccggttgc ggaaacttcg
     2881 gtctgcgcgc gattgtaatg aacgcctatg ccgaactggg attgaaaaaa gaaaatctgg
     2941 tgatggtgtc cggtatcggt caggccgcca aatcaccgca ctattatgac gtcagctatt
     3001 tcaacggcct gcatggccgg gcgctgccgg cggcgaccgg catcaaagcc gccaatccgg
     3061 aactgacggt ggtggtgcaa agcggcgacg gcgatatgta cggcgaaggc ggcaaccatt
     3121 ttctgcacac catccgccgc aatccggaca tcaccgtgat cgtgcatgac aatatggtgt
     3181 acgggctgac caaaggtcag gccgcgccga cttcgcaaat cggcatgaaa acgccggtgc
     3241 agattgacgg cgttttcgaa gagccgatca atccgctgac gctggccatt tcgcttggcg
     3301 ccacgtttgt ggcgcagggg tcagccggcg aacgcgaact gaccacggaa ctcattaagc
     3361 agggtatccg gcacaagggg ttcgctttaa ttaatgtatt tcaggcctgt ccgtcgttca
     3421 acaagctgaa caccgcgcag tggttcaaag ataacatccg cgcagtgccg gacgactacg
     3481 atccgtcaga caaagcggcg gctttccgta aagcgctcga gaccgtcccg tggcccgtcg
     3541 gtgtcattta tcgggaaacc ggcaagccga cctttgaaga ccatattcat aaaaacgaca
     3601 gtactccgct ctaccgccgc ccgcgttcta cggaacgcgt aaaaaaactg ctggaaagca
     3661 aacgataatc tacgcctgct tggtgcgaag cagttcggca tcggcttcag caaaaagatt
     3721 ttccgacggc agcgcggcta tccccgccga aactccggcc agcggttccg ccatcagaaa
     3781 aaaacgttcg gtgaatttac gggcactggc cgctccggtc tccggcagaa tgacggcgaa
     3841 ttcgtcgccg ccgatgcggc agacgatgtc ggatttgcgc gccgcggccc ggagtgtctg
     3901 tgcaaaacct ctcagcaccg catcgccggc ttcgtgtccg cgggtgtcgt tgacggcttt
     3961 gaaatcatcc agatccaaaa gcaccagact gagcccgcgg ccgtaccgcc gggcggcttc
     4021 aacctgctgc gccagggctt catcaaaaaa gcggcggttg tataaacctg tcaacggatc
     4081 acgtaccgcc tgttcggcca gttctgcggc aagctcgagc acatcggcgg gtgcgccgat
     4141 ttcggcaagg cgctgctgga ggcggtcaga catgggtcag caaaaagaat tgcgcataga
     4201 tccaggaaat gaactctgta acaacgtgct tgaagccggc actgtgccgg taccagtgac
     4261 taagattata ggaaggcggc tcaacggcaa tgacacccag cggatagttt cttccgaaga
     4321 catggcggaa aagaagatag gaccggcggg cgtgcgcgcc cacggtataa aggttggcgg
     4381 atttcccgaa aagtccgagt tcttcaagct ttgcgcgagc cgccacagcg gaaacatagg
     4441 tgcggtcgcg ctgcgtgtcg ggagccggaa tggttataat ggaggaggcc ggaattccgt
     4501 actcaaccag cctcgccgtt ccaagttctg cataattatc atactgaagg atcttctgcc
     4561 cgaaagtaac aggtcctccg gttgtgacaa caatctgtcc gggctgaata gcgccggccg
     4621 ccgctttaag ctccgcatca gccatccagc cttcgatgat aatgattccg gcagccggct
     4681 gaggatggtt ctgcgccaga aatggataca aatgacagac ggctccgcaa acaagcgccc
     4741 cgacaagcag aatccccgaa acaaagccct gccaggtggg catccagacc tcttttttgc
     4801 agacgagctt catccggacg ggtataacct ttcggacagt caggaacaat tcatatcttt
     4861 ttgcataaaa cttttggtac gggggttgac acggggtatc ccctcgtata ttttccccgc
     4921 cttgcatttg aactggaaag atttgtaatg aagacatttg tagcaaacga aaacgatatc
     4981 gaacgggaat ggttcgtggt agacgccacg gataaatcaa ccggacgtct ggctgtattc
     5041 atcgccgacc ttctgcgcgg ccgcaacaaa ccgacctaca ctcctcatgt ggacacaggc
     5101 gcctttgtgg tggtcatcaa cgctgaaaag gtgaagctga ccggcaataa ggaagagaat
     5161 aaaatttatc aggacttcag cggctatccg agcggccagc agaatcgcag cgccgccgtg
     5221 atccgcgaaa agaatccgac gcgtattatt acgcaggctg ttgaaggcat gctgccgggc
     5281 aaccgtctga tgcgccgtac aatcacccgc ctgaaagtct acacgggtcc caaccacccg
     5341 cattcggctc agaaggttac gacactcgaa tttaaaggct aataaggagt aaaacgtggt
     5401 agcagcgaaa aaagtcgatc aaattgccgc aacaggacgg cgcaaaacat ccgtagcgcg
     5461 cgtcagcctc gttaaaggct ccggtgttat caccgtaaac ggcaaaaccg ccaaagatta
     5521 ttttgatacc aaggacatgc tggataccgt cgtacgtccg ctggtactca ccggacgttc
     5581 cgatcagtat gatatgacgg tcacagtcaa aggcggcggc aaggtcggcc agtgcggagc
     5641 catttcccac ggcgcctcgc gggctctggt taaagcagaa gaagagctga aaccgatcct
     5701 caaaaaaggc ggcttcctga cccgcgatgc gcgtatgaag gaacgtaaga aaccgggcca
     5761 gccgggcgcg cgcaaacgct tccagttctc gaaacgttaa gccgccagcg gcttacggca
     5821 aagtcccggg ctcctgcgag accgggactt tttttattcc gaaaacatct gcggcggtgt
     5881 agtcttccgc ccttggagtt tggaaagcat gaaaaagaca ctgagtttac caaaaggatt
     5941 ttccgcggcc ggcatcgccg caggaatcaa gaaaaagaaa aaagacatgg ccctcatcgt
     6001 ctcagacgta atggccgaaa gcgcagctgt ttttaccacc aatcaggtta aagcggctcc
     6061 cgtcaagtgg gacatcaagg tcgtaaaaca tgacggcgcg cgggcgattg tgatgaacag
     6121 cggcaacgcc aatgcctgca ccggcgcaca gggcatggct gacgtcgaag cgactgcggc
     6181 tctggccgcc gggaaactcg gcatcaattc cctggatgtt tttgtctgct ccaccggcac
     6241 catcggaaag ccgcttccga tggataaaat cgccgccggc attgagactt tattcgacga
     6301 ggtatcaccg gatggcggca tggacgcggc ggaagcgatg atgaccaccg acctcgtcag
     6361 caaaacggtc gtggcggaaa tcgatctcga cggcgtaccg gtacggatta ccggtctggc
     6421 caaaggctcc ggtatgatcg aaccgaacat ggccactatg ctcgccttta ttctgacgga
     6481 tgcggcagtc gaaaaacaca ccttgcaatg cgcactgcgc accgcagccg atatgagctt
     6541 caaccggatc accgtcgacg gagaccggag tacaaacgat acggtcatct gtctcgcaaa
     6601 cggccgggcc ggcaatgaaa cgattactca aagatcaacc cattggccgc tcttccttca
     6661 aacgctggaa aaggtgcttt ttgaactctc gatgatgatt gtgaaagacg gcgaaggcgc
     6721 tacccgggtc atcaccgtca aagttaacgg cgcggcatcc aacggagaag ccgaggaagc
     6781 cgcgcgggca gttgccaact ccatgctcaa caaaacagcc tgggcaggca agcggcccaa
     6841 ctggggccgg attatggacg ccatcggcta ttcctacgca caggtgcagg aaaacaaggt
     6901 cgatatcgat tacgacgatg ttcccgccgt acggggcggt atggccgccg gaacgccgga
     6961 agaggaactg gtcgcggcgg tttcgaaaga agcgttcacc ataaatatcg acctgaatct
     7021 cggtacaggc cacaccgtgg tatacacctg caactgcacc gaagactatg tacgaatcaa
     7081 cgttgaatag gacgaagaag aaccatgcag cagatgattg aaaaagcggc ggtgctgatc
     7141 gaagccctgc cctatattca gaagttccgg ggtgatacgg ttgtcgtcaa attcggcggc
     7201 agcatcatgg agagcgaaat cggctaccgg aatatcctca aagacgtcgc gttcatggaa
     7261 tgcgtcggcc ttcagccggt cattgtacac ggcggcggaa aatcggtttc caagaaaatg
     7321 cgggaagccc acatccagcc gaattttgtt cagggcctgc gggtcaccga tgcggcaacc
     7381 atcgaagtcg tagaaaaagt tctgaataac gaagtgaatc cccatctggt cgaaattctg
     7441 aaaaattacg gcggaaaagc acgcggcatt cacggcgagg atatcattac ggttaaaaaa
     7501 cataccggca cagacccgca gacgggcgag gaactcgact ggggttacgt cggtaaagtc
     7561 attcatgtgg atgtcgagcc gatcaaagcc tttctcaatg ccgacatcat tccggtcatc
     7621 acaccgctcg gacgcggcga agacggacac ctctacaacg taaacgccga cgacgtcgcc
     7681 aacgccattg ccgccgctct tcaggcgcgc aaactggtct tcctgagcga tgtaccgggg
     7741 ctgctgcggg acgtgaacga tccggcttcg ctgatttcga ccttgagtct ggccgaagtc
     7801 gaagacctga tcaaacgggg aattatctcc ggcggtatgc tgccgaaaat cggcggcgca
     7861 gtggacgcac tcaaagcggg cgtgaacaaa acgcatatca ttgattcagc gctgccccac
     7921 tcattgctgc tggaactttt caccgacaaa ggcgtcggaa ccgagattgt gaagtgacgt
     7981 ttgccggctc cggcttattc tgacggcgaa gaaatacgga atgcctttta ggggtgaggc
     8041 cctgccggga caaagttagg acgggtagat gtgcaataat atctgcatgc ttgtcggctc
     8101 aaggaaacgc aaagtatcgc gtacgtgtcg ctttggtgcg ttccttttca tcggtctgtc
     8161 gggattcctc ctgcctctgc cggcccggcc ggctgaacag ccagcaagtc cgccggtttc
     8221 aaccaactat caggggctga gaaccatcct cagctaccaa cccctccccc gccgttccgg
     8281 cacatcccgg gagaccgacc cggaccctgt ccgggtgatg cccattagca aggagctgcg
     8341 ccgcacctat cacatcgacc ccttttatac gaaaaccatc accagcgccg gcattgcgat
     8401 tgtgggctcg gacaaagtct ccgactgggc atttctggag gcggcctaca cgatggacca
     8461 cgtactgtac gaaagtcccg cctgggttcg ggaagcgctg gccgcaacaa aagtgcgaat
     8521 ggccattctg gcgtgtgttg aatacaccat ggatctgccg gaaaacaacc gcacgccccg
     8581 tacgctggcc gaagcggcgt tccaggatca gcgctcacgc ggtcttggcg gcctgcccga
     8641 ttgcagctgc gccgaagaaa acctgctgaa cctgcgcggc gatccgtacg acggcgatgg
     8701 atcgaccgat gccggcgaaa gcattaccat ccacgaattt tctcacacgc tggccggtac
     8761 aatcagaaaa taccaaggcc tcaacgggga cttctggacg aaactgaaca aggccttcgc
     8821 cgacgccaca gccccggatg gatatctcgg gattcagaat aaaggccgga aggttaaaat
     8881 ttacgcctcc cagagcgcac aggaatattg ggcggaaggc gctcaagcct ggtttgaccg
     8941 cgccgaccgg cgcagctcgg gcggcctgca cacgcgcgac gacgttaagc gacaagatcc
     9001 ccagctggca gcactgctgg cggaaattta cggcgacggc ccctggagat atcacaagac
     9061 cacagccaaa aagccggacg ggacgcccat gcgcactccg gacgaattgg cccatctgga
     9121 gggtctggat gatctgagga aacagttccc ggcctacaac aaaaataatt cgccgcgcat
     9181 catggctctg atgcaggaaa ccgcggcccg gacggcggaa gaggccggac ggaacgccgt
     9241 caccaacgcc ccggccccct cgacgccgga aagccggcca tccgctcgct gatgctcatc
     9301 gggcctgcaa tccccgcatc gcaattactt accgtctgcg cgagcttcta tcgtttaaac
     9361 cactggcatt cccggcttgt cgcctctaaa gaactgcatt agcttcaaac cgtttagaat
     9421 caagcccgca aggaaactaa agtagagcta ataacactgt taaccgcgcc ccgcgctggc
     9481 gcgcggttcg cggcagctca gttccggcgt tgggtgtgga tacgaattat gaaaaagatt
     9541 ttagcattaa gaataattga aaccattttc ctattcggga ttgtgttcat aaaaacaatc
     9601 actcctgagc agtggtctaa atcgacttcc tatcttgctg caggcttcca tctcaatctt
     9661 tcgacaaatg ctgacactta cattttcagt acattattga tcggttatgt agtcagcttg
     9721 atcgcatcat tcatgcagag aggtattaaa aatgagtgcc tcaaaccctc ttttgttttt
     9781 gccattcttg gcgtggcagg tttttcaaac gaaatcttgc ggtacataat cgagtacagg
     9841 tttcaactga tactctcatt ccctattcta cttctgattt tcgactggtt ggcatttttc
     9901 agactattaa aaaaatcacc caaccagaac atcgagccta cgctgaaaac accggttgag
     9961 tcaggcgaag ttgaatccgg cgaggctcac gtttaacgtt cgccaataat atgagaagcc
    10021 caatgccatc ttggagtatt ccgagcaatc tggaagaaat catcgattca gacgacggtg
    10081 gaatttggga tgatgccgag acatttgcgc cgatgaccct ctccgtgatg aagggcacag
    10141 agtatgatgg tcgtgagatt ccgcacgcgt ggcagattga attcgagccg gagagtgaag
    10201 agtgcgagat caccaatgaa cgcatttcga gcatgggtct tgaggccgac ggctatgggt
    10261 gggccacgtt gatcgatagc gtcgttcgca agtatcaccc tgagatcgcc gacgaactga
    10321 actttggaga caccgatacc gcttccttgg tggtttgggt tgagtccgag gcgacttgcc
    10381 ggaaggtcgc tgaggtggcg tggaacttga tcttcgcaac gtgaacccaa cagaatccaa
    10441 gcaggcgaac aaggcgctcg tggcaacggg cgataacgtc tcaagttgaa ttcgagcttc
    10501 catttcgccc gtgccacagc tagacgttca tctgtaacga tttaccggcc cgctcccttt
    10561 tctgtcgttt aaaccactgg caattccggc ggacgtgcgt tacattcgcc cgtctttgct
    10621 ggaagtctgt ttcacaaaac gtttgccccc tgcccgaagg ttttaaatat gccgagttca
    10681 acaacctcga aaggaaacat catgaataca ttgttaaaag tgatg
//
LOCUS       DAOPZO010000062        10482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_7364_length_10482_cov_7.917330, whole genome shotgun sequence.
ACCESSION   DAOPZO010000062 DAOPZO010000000
VERSION     DAOPZO010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 10482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10482
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7364_length_10482_cov_7.917330"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /gene="tyrS"
                     /locus_tag="PKI68_05365"
     CDS             complement(<1..1076)
                     /gene="tyrS"
                     /locus_tag="PKI68_05365"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNX53153.1"
                     /translation="MNTDEQLSQLTARHVDLFSAGELKRKLEAAKKEKRSLRIKYGAD
                     PSAPDIHLGHVVGLNKLREFQDAGHTVVFIIGDFTGMIGDPSGKSATRPALSREQVAA
                     NAESYKQQVFKILDPAKTEVRFNSEWLGGMKFEDVIRLSAHVTVAQLLARDDFSKRYA
                     DNRPISLVEFLYPLVQAYDSVMVKADVELGGTDQLFNLLLGRELQKVYGQEPQVVMTL
                     PLIEGLDGVQKMSKSLGNYVGVNECAKDMFGKLMSVSDELMWKYFLHILCRKPEEIQA
                     MQAKVASGELHPRAVKDELGRCVVDRFVGAGEGQKASDEFARIFSKNELPDDMPDLKL
                     AGDTGIIDLVMKAGFAPSSSEARRL"
     gene            1283..2749
                     /locus_tag="PKI68_05370"
     CDS             1283..2749
                     /locus_tag="PKI68_05370"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNX53154.1"
                     /translation="MTKNVSRLFYAGSLILSLAATPLSQAAEPYTADWDSLRKHPPVP
                     EWLRDAKLGFYATWGVYCVPAFGNEWYPSYMFAKEGKNNKLGVYAHHQKTYGDPSKFG
                     YHDFVPMFTAEKFNADEWADLYQKSGAKFGGTVTEHHDGFSLWASKVNPWNAKDMGPQ
                     RDIIGELEKAIRARGMKFIVTMHHARMYGMREGRVYWTKAPGWPTSSDDPKLRILYGN
                     MPKEEGIKLWRSKLDEVLDAYRPDMTWFDIDLELVPDQDKREFIASYFNKAQGWGKEV
                     LVTYKLTQLPPGIGLLDYENGRASKLMAEPWLTDDTIGVVKSVWSYVPDMKIKSTQAL
                     VHELIDIVSKNGQLLLNMSPRADGSIPDDQRASMLGLGRWLAKYGEAIYYTRPWLIYG
                     EGKKSAAGFPANDNEIRYTAHANKIYASLLNGKLNGERLVLHAFAEGGAGAACKITDV
                     KLMGSDAKIAWTRTPDGLEITLPSPLPDPDANVLVLTK"
     gene            complement(3004..4737)
                     /locus_tag="PKI68_05375"
     CDS             complement(3004..4737)
                     /locus_tag="PKI68_05375"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNX53155.1"
                     /translation="MEQTLKTDGFSIGLDAAAIDAMYNNTLKDFTVGSIVPGKVLRVQ
                     DDEVLVDIGYKSEGIVDGDEFEDVDAVKVGDTIEVFLESLENDHGMVVLSKKRAEQQR
                     KWDHIVNDCEEGSLVEGTITRAIKGGFIVDIGADAFLPGSQVDVVPVRNLDDYIGRTL
                     QVKIIKINKERKNIVVSRREIIEETRREQKLKLLGEIKVGQLRKGTVKNITDFGAFVD
                     LDGIDGLLHVTDMSWGRINHPTEMVKVGDEIEVMILDINLDKERISLGLKQTLSNPWQ
                     DIDAKYPIGTRVHGKVVSLAPYGAFVEVEEGVEGLVHVSEISWTKRIQRASDVLKVGD
                     EVDAVVLSINTEDKKISLGMRQTEENPWEVAAHKYPIGSLVRGKVRNFTSYGAFVEIE
                     EGVDGMIHVSDMSWTRKINHPSEVLKKGEEVETIVLEIDATNQRISLGLKQAQEDPWA
                     RITDAYKEGQKITGKVTKLTNFGAFVELEDGIDGLVHISQISDAHVEKVKDVLNIGDQ
                     VEARIVKIDPVEHRIGLSIKAAKIADEDFEISEELLTGLRPGEELVDLSSAFDGLLTG
                     NEEWHPGQKSE"
     gene            complement(4979..5725)
                     /gene="map"
                     /locus_tag="PKI68_05380"
     CDS             complement(4979..5725)
                     /gene="map"
                     /locus_tag="PKI68_05380"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNX53156.1"
                     /translation="MIVIKTPDELDAMRRVNRMTARVRDEIAAMVRPGISTIELGNAA
                     FEIIRKLGGTSAFYGYHGFPGQICVSVNEEVVHGIPGKRVIRAGDIVSIDTGITFGGF
                     IGDTAVTVAAGEISDDKKKLLQVTKAALDAGIAKAVAGNHLGDISHAIQTVVEAAGFS
                     IVREFVGHGIGRDMHEDPQIPNFGRPGRGPKLKPGMTLAIEPMVNIGGPKVKVLDDNW
                     TVVTCDGSASAHFEHTVAVGVTAPEILTLS"
     gene            complement(5734..6381)
                     /locus_tag="PKI68_05385"
     CDS             complement(5734..6381)
                     /locus_tag="PKI68_05385"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNX53157.1"
                     /translation="MEAIILLGGPGAGKGTLAEFLKKQTGYIHVSTGDMLRAAIQAGS
                     PVGLEAKGFMDAGQLVPDAVVLRIIAERISKEPADARFMFDGFPRTIDQAEGLDQLFK
                     KQGGVINHVFDLVVPCGVILRRLTGRRICKSCGAVYHITNRPPKKEGICDLDAGELYQ
                     RVDDSEATILNRLEVYEKQTAPLISYYQRQGLLREIAADCNAVETDALVLKILNA"
     gene            complement(6385..7728)
                     /gene="secY"
                     /locus_tag="PKI68_05390"
     CDS             complement(6385..7728)
                     /gene="secY"
                     /locus_tag="PKI68_05390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNX53158.1"
                     /translation="MLSAFVNSFKIQELRQRILFTLGVIFLCRLVSDIPIAGVDFSMI
                     SSYIKSSAGQGTGFLDMFNLFSGGAVANCAVGALGIMPYISASIIIQLMTAVVPALGK
                     LVREGEAGRAKITQYTRYLTVALCAFQGFLIARALESGQLYFPADVVRIPGFGFELLT
                     TMTLVTGTLLLMWLGEQITERGIGNGISMIITVNIVSRMPTAVSAMISMFKPVDGVAR
                     HSLFMAATLLIMVLAVIAVVVAVTQAQQKIPVQYAKRVVGRKVYGGQHTFMPLRVNYA
                     GVMPIIFAQAILMFPEKIFNMMPWAAAKQLGAALGYGTGLYMVCYGLMILFFSYFWVA
                     TQFKPVQIADDLKKYGGYIPGIRPGKSTAEFLDRTMTRITLAGAVFLTGIAVLPTVMG
                     NQFGIPAMVASFFGGTSLLIIVGVMLDTMRQIESHLLMRHYDGFLKKGKLKSGRR"
     gene            complement(7753..8193)
                     /gene="rplO"
                     /locus_tag="PKI68_05395"
     CDS             complement(7753..8193)
                     /gene="rplO"
                     /locus_tag="PKI68_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNX53159.1"
                     /translation="MELHSLQPAKGSTHRKMRVGRGRASGKGKTSGRGHKGAYARSGH
                     KHKPLFEGGQMPMARRIPKRGFSNYTRKVLAPVNVAALNAFEAGTVVTLDLLRQVKLV
                     NGQWDGVKVLGNGEISKKLSVKACAFSASAKEKIEAAGGTCEIV"
     gene            complement(8212..8697)
                     /gene="rpsE"
                     /locus_tag="PKI68_05400"
     CDS             complement(8212..8697)
                     /gene="rpsE"
                     /locus_tag="PKI68_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNX53160.1"
                     /translation="MEKSAEKKVKSEFEERVVHINRNSKVVKGGRRFSFGALVVVGDG
                     AGRVGYGLGKANEVADAIRKGGEAAKRAMTPVVMRGTTIPHETTGKFGGAQVLLRPAS
                     EGTGVIAGGSCRAVLELAGVRDVLAKSLGSSNKLNVTKATMAALATLRTREAALAARG
                     K"
     gene            complement(8728..9069)
                     /gene="rplR"
                     /locus_tag="PKI68_05405"
     CDS             complement(8728..9069)
                     /gene="rplR"
                     /locus_tag="PKI68_05405"
                     /inference="COORDINATES: protein motif:HMM:NF013057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNX53161.1"
                     /translation="MKVKNKSDYRKRRHLRLRNKVQGTAERPRMAVYVSNRYIYVQVI
                     DDAAQKTLCSVSSLGEKCTVDTAKALGTKAAAVAKEQGVSKVVFDRGGFAYGSRLRAL
                     ADSAREAGLQF"
     gene            complement(9072..9608)
                     /gene="rplF"
                     /locus_tag="PKI68_05410"
     CDS             complement(9072..9608)
                     /gene="rplF"
                     /locus_tag="PKI68_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNX53162.1"
                     /translation="MSRIGKMPVTVSGGVEVTLSGNVVTVKGAKGQSTLQIPALITVA
                     VEGGRVTVGRKNETKEARALHGTIRNTIKNMILGVQSGYAKELEIQGVGYKAQLQGKI
                     LVLSLGFSHDIRYEIPEGIKVEVAGATDVKVSGHEKALVGLVASRIRAFAPAEPYKGK
                     GVRYKGEQVRRKEGKAVA"
     gene            complement(9630..10028)
                     /gene="rpsH"
                     /locus_tag="PKI68_05415"
     CDS             complement(9630..10028)
                     /gene="rpsH"
                     /locus_tag="PKI68_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNX53163.1"
                     /translation="MILSDPISDMLTRIRNANMAAHTKVQMPHSKMKSELARILKQEG
                     YIKDYTTEKVEGKPTLVLHLKYQDDQKPVIQGLRRVSRPSCHHYVMADDVPRVLGGIG
                     LAILSTSSGMLSDKEARKAHVGGEVLCYVW"
     gene            complement(10025..10480)
                     /gene="rplE"
                     /locus_tag="PKI68_05420"
     CDS             complement(10025..10480)
                     /gene="rplE"
                     /locus_tag="PKI68_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNX53164.1"
                     /translation="MNVSVGMQHDRDALQAVLDDLAKITGQHPVTIKARKSVSNFKLR
                     EGVSLAGKVTLRGDRMYEFLDRLINIALPRIRDFRGIPPKSFDGFGNYSLGLKEQTIF
                     PEINPDHVRKVHGMDVTFVTTAKTNAEAYELLSLIGLPFETAKNKGEQQ"
BASE COUNT         2329 a         2965 c         2806 g         2382 t
ORIGIN      
        1 aggcggcggg cttcgctgct cgacggcgcg aagccggctt tcatgacgag gtcgatgatt
       61 ccggtgtcgc cagccagttt caggtcgggc atgtcgtccg gcagttcgtt cttggaaaag
      121 atacgggcga attcatcgga ggctttctgc ccttcgccgg cgccgacaaa ccggtcgacg
      181 acacagcggc cgagctcgtc tttgacagcg cgcggatgca gttcgccgga agccactttg
      241 gcctgcatgg cttgaatctc ttccggtttg cggcagagaa tgtgcagaaa atatttccac
      301 atcagttcgt cgctgacgct catcagcttg ccgaacatat ctttggcgca ttcgttgacg
      361 ccgacgtagt tgccgaggct cttgctcatt ttctgcacgc cgtcgaggcc ttcgatcagc
      421 ggcagcgtca tgacgacctg cggctcctgg ccgtatactt tttgcagttc gcggccgagc
      481 agcaggttaa agagctggtc ggttccgccg agttcgacgt cggctttgac catgacggag
      541 tcgtaggcct gcaccagcgg gtagagaaat tcgacgagcg agatcgggcg gttatcggcg
      601 tagcgttttg aaaagtcgtc gcgcgccagc agctgagcca ccgtgacgtg tgcggaaagg
      661 cggatgacgt cttcaaactt catgccgccg agccattcgg agttgaagcg gacttcggtt
      721 ttggccggat cgaggatttt aaaaacctgc tgtttgtagc tttcggcatt ggcggcgacc
      781 tgttcgcgcg aaagcgccgg acgggttgcc gatttgccgg acggatcgcc gatcatgccg
      841 gtaaaatcgc cgatgatgaa tacaacggtg tgcccggcat cctgaaattc gcgcagcttg
      901 ttcagaccga caacatggcc gaggtgaata tccggcgcgg acggatcggc accgtacttg
      961 atgcgcaggc tgcgcttttc cttttttgcg gcttcaagtt ttcgtttcag ttcgccggcg
     1021 gaaaaaagat ccacgtggcg ggcggtgagc tgactgagct gttcgtctgt attcataagt
     1081 ggccggagaa taaaaaaacc agccgctatt tacaacctgc gggttgataa aagagctcag
     1141 gctcagccaa acatctgttc atcacgacca acccggctgc ggatgccccc ccccgccttg
     1201 actcgttcag tgttttgaac tagctttcgg ctctaaaatc ggcgccgagc gctgcgagaa
     1261 gtgaaaactt atcaggagaa ctatgacgaa aaatgtaagt cggttatttt atgcaggatc
     1321 gctgatcctg tcgctcgcgg caacgccgct cagtcaggct gcagaaccct acacagcgga
     1381 ctgggactcg ctccggaagc acccgccagt ccccgaatgg ctgcgcgacg ccaagctcgg
     1441 attttatgcc acatggggtg tgtactgcgt cccggccttc ggcaatgaat ggtatccgag
     1501 ctacatgttc gccaaggaag gcaaaaacaa caaactgggc gtctacgcgc atcaccagaa
     1561 aacctatggc gatccctcca aattcggtta tcacgacttt gtcccgatgt tcaccgccga
     1621 gaagtttaac gccgacgagt gggccgactt ataccagaag tccggcgcaa aattcggcgg
     1681 aaccgtcacc gagcaccacg acggcttctc gttgtgggcc agcaaggtca atccgtggaa
     1741 cgccaaagat atgggcccgc agcgcgacat tatcggcgag ctggaaaaag ccattcgcgc
     1801 gcgcggcatg aaatttatcg tgacgatgca ccacgcgcgc atgtacggca tgcgcgaagg
     1861 ccgcgtctac tggaccaaag ccccgggctg gccgacctcc tcggacgatc cgaagctgcg
     1921 catcctgtac ggaaacatgc cgaaagaaga aggcatcaaa ctctggcgca gcaaactgga
     1981 tgaagtgctg gatgcctacc gcccggacat gacctggttc gacatcgatc tggaactggt
     2041 ccccgaccag gacaaacgcg aatttatcgc cagctatttc aacaaggctc agggctgggg
     2101 caaagaggtg ctggtcacct ataagctcac gcaacttccc cccggcatcg gcctgctgga
     2161 ctatgaaaat ggccgcgcct caaagcttat ggccgaaccc tggctgacgg atgatacgat
     2221 cggcgtcgtt aaaagcgtct ggtcgtatgt tcccgacatg aaaatcaaat ccacccaggc
     2281 cctggtgcat gaactgatcg atattgtcag caagaacggc cagctgctgc tgaacatgtc
     2341 cccgcgggcg gacggcagca ttccggacga tcagcgcgcc agcatgctcg gcctgggccg
     2401 gtggctcgcc aagtacggcg aagcgattta ttacacccgc ccgtggctga tctacggcga
     2461 aggtaaaaag agcgcagccg gcttcccggc caacgacaac gaaatccgct acaccgccca
     2521 cgccaacaaa atttacgcca gcctgctgaa cggcaagctg aacggcgagc ggctggtgct
     2581 tcacgccttc gccgaaggcg gcgcaggcgc cgcgtgcaaa atcaccgacg tcaagctgat
     2641 ggggagcgat gccaaaatcg cgtggacccg caccccggac ggactggaaa tcacacttcc
     2701 ctcgccgctg cccgacccgg atgccaacgt cctggtgctg acgaagtaat caagaaaaca
     2761 aggcaacgcc cgctgctccc ggcagggcgt tgccgccgct cccgccttca ggccatttct
     2821 ccgggccact cccgcttgcg gcacatcaca gacgcggaat gatcgaacac ggccccgtcc
     2881 agatcgcgtg ggcttgaaca atgttaacag ccggctccac cgggaaagcc gccgcacctc
     2941 cacaacaaaa aaccccgcgc cgggaatccg ggcggggttt ctaaacggag tcagtgctcc
     3001 gctttattcg gatttctggc cgggatgcca ctcttcgttg ccggtcagca gaccgtcaaa
     3061 ggccgaggag aggtccacca gctcttcgcc cggacgcaga ccggtgagca gctcttcgct
     3121 gatctcgaag tcttcgtccg caatcttggc ggccttgatg ctgagaccga tgcggtgttc
     3181 aaccgggtcg atcttaacaa tgcgggcttc cacctggtcg ccgatgttga gcacatcttt
     3241 gactttttcg acgtgcgcat cgctgatctg gctgatgtgc accaggccgt cgataccgtc
     3301 ttcgagttca acaaacgcac cgaagttggt gagcttggtg actttgccgg tgatcttctg
     3361 gccttctttg taggcgtcgg tgatgcgggc ccacggatct tcctgagcct gcttgaggcc
     3421 gagggagatg cgctggttgg tggcatcgat ttcgagcacg atggtttcga cttcttcgcc
     3481 ttttttgagg acttcggaag gatggttgat tttgcgcgtc cacgacatgt cggagacgtg
     3541 gatcatacca tcaacacctt cttcgatttc gacgaaggcg ccataggagg tgaagttgcg
     3601 aactttaccg cggaccagcg aaccgatcgg gtatttgtgc gcagcgactt cccacgggtt
     3661 ttcttcggtc tggcgcatac cgagcgagat cttcttgtct tcggtattga tcgacagaac
     3721 aacggcatcc acttcgtcgc cgactttgag aacgtcggaa gcgcgctgaa tgcgtttggt
     3781 ccaggaaatt tcggaaacgt gcacgagacc ttcaacgcct tcttcgactt caacgaaagc
     3841 gccgtacggg gccaggctga cgactttacc gtgaacgcgg gtgccgatcg ggtatttggc
     3901 gtcgatatcc tgccacgggt tggagagagt ctgtttcagg ccgagcgaga tgcgttcctt
     3961 gtcgaggttg atgtcgagaa tcatcacttc gatctcgtcg ccaactttga ccatttcggt
     4021 cgggtggttg atgcggcccc agctcatgtc ggtgacatgc agcaagccgt cgataccgtc
     4081 gagatccaca aacgcaccga aatcggtgat gttcttgacg gtgcccttgc ggagctgacc
     4141 gactttgatt tcgccgagca gtttgagttt ctgttcgcgg cgggtttctt cgatgatctc
     4201 gcggcgggaa accacaatgt ttttgcgctc tttgttgatt ttgatgattt tgacctggag
     4261 cgtccggccg atgtagtcat cgaggttgcg caccggaacg acatccacct gggagcccgg
     4321 caggaatgca tcggcaccga tatcgacaat gaagccgcct ttgatggcgc gggtgatggt
     4381 tccttcgacg aggctgcctt cttcgcagtc gttgacgatg tgatcccact tgcgctgctg
     4441 ttccgcgcgc ttcttgctga gaacaaccat tccgtggtca ttttccaggc tttcgagaaa
     4501 tacttcgatg gtgtcgccga cttttacggc atccacgtct tcgaattcgt cgccgtctac
     4561 aataccttcc gatttgtagc cgatgtcgac aaggacttcg tcgtcttgaa cacgaagcac
     4621 tttaccgggc acgattgagc cgacggtgaa gtccttcagg gtgttgttgt acattgcatc
     4681 gatggcggcg gcatccaggc cgattgaaaa gccatcggtt ttgagtgtct gttccatggt
     4741 ttctgttttc tgttctatcc ggcgacgtcc gcatccgcat tgcgcggcgc gccccgtccc
     4801 ggggttgtgt cttttcccgc agaacctgct cacggccacc cggccggtta gttcacaact
     4861 ctgcggtttg taaaagagcg gaaatcatag tgagccgggc ttcaaaaaca aggctcaaat
     4921 gcgtttttaa gcctcatata ttcgagggaa acacttccac gccgccaaca tcaggagatc
     4981 aggaaagcgt aagaatttcc ggcgcggtta cgccgacagc gacggtatgc tcaaagtgcg
     5041 ccgaggcgct tccgtcacag gtgacaacag tccagttgtc gtccagaact tttaccttcg
     5101 gaccgccgat gtttaccatc ggctcaatgg cgagcgtcat gccgggcttg agtttcggcc
     5161 cgcggccagg tctgccgaaa ttcggaatct gcggatcttc atgcatatcg cggccgatgc
     5221 catgtccgac gaattcgcgc acgatagaaa aaccggcagc ctcaacaacc gtctgaatgg
     5281 catgagaaat atcacccaga tgattaccgg ccaccgcctt ggcaattccg gcatcaagcg
     5341 cggctttcgt gacttgcagc agcttctttt tgtcgtcgct gatttcgccc gccgccaccg
     5401 tgacggcggt gtcgccgata aagccgccga aggtaatgcc ggtgtcgata ctgacaatat
     5461 cgccggcccg aatcacccgc ttgccgggaa taccgtgaac gacctcttca ttgacggaaa
     5521 cgcagatttg tccgggaaag ccgtgatagc cgtaaaaggc gctggttccg cccagcttgc
     5581 ggataatttc gaaggctgca ttacccagct caatcgtaga tataccgggg cgaaccatcg
     5641 cagcgatttc gtcccgaacg cgcgccgtca tgcggttaac ccggcgcatc gcatcaagct
     5701 cgtcaggggt tttgattaca atcatcagaa cccttacgca ttgagaattt taagaacaag
     5761 cgcatcggtt tccacagcat tgcagtcggc ggcgatttcg cggagaagac cctgccgctg
     5821 atagtagctg atcaacggtg cggtctgttt ttcgtaaact tcaagccggt tgagaatggt
     5881 ggcctcgcta tcgtcaacgc gctggtaaag ttcgccggca tccagatcac aaatgccctc
     5941 ttttttcggc gggcgattgg ttatgtgata gacagctccg caacttttac agatgcggcg
     6001 tccggtcaga cgtctcaaaa taactccgca gggaacaacc agatcaaaga cgtggttaat
     6061 gaccccgccc tgttttttaa ataattgatc aagcccttcg gcctgatcaa ttgtgcgcgg
     6121 gaacccgtcg aacataaaac gggcatccgc cggctctttg gaaatccgtt ccgcaataat
     6181 tcgcagaaca actgcatcag gaacgagctg accggcgtcc atgaacccct tggcttcgag
     6241 gccgaccggc gaaccggcct gaatggcggc acgaagcatg tcgccggtag agacgtgaat
     6301 ataaccggtc tgctttttta ggaattcagc cagagtgcct ttgccggccc ccgggccgcc
     6361 aaggaggatg atcgcttcca tcgcctacct tcttccggat ttgagttttc cttttttcaa
     6421 aaatccgtcg tagtgtcgca tcagcaggtg agactcgatc tggcgcatgg tatcgagcat
     6481 aacccctaca ataataagca ggctggtacc gccgaaaaag gatgcaacca ttgcaggaat
     6541 tccaaactgg ttgcccataa cggttggaag cacagcgata cccgtcagga aaacagcacc
     6601 cgccaaagtg atgcgggtca tggtgcggtc aagaaattct gcggttgatt tgccgggacg
     6661 gataccggga atatatccgc cgtatttttt gagatcgtcg gcaatctgca caggtttaaa
     6721 ctgtgtagcc acccagaaat aagaaaagaa aaggatcatc aggccataac aaaccatgta
     6781 gagccctgtt ccgtagccca gcgccgcacc gagctgcttg gcagccgccc aaggcatcat
     6841 attaaatatt ttctcaggaa acatcagaat agcctgggcg aaaatgatgg gcatcacgcc
     6901 ggcatagtta acgcgcaacg gcatgaatgt gtgctgtcca ccgtaaactt tacggcccac
     6961 aacacgtttt gcgtattgaa ccgggatttt ctgctgtgcc tgagtaacag caacaacgac
     7021 agcaataaca gctaaaacca ttatgagcaa cgtagccgcc atgaagagcg aatggcgagc
     7081 aacaccgtca accggtttaa acatgctgat cattgcgctt accgcagtcg gcatacgact
     7141 gacgatattg acagtgatga tcatggaaat accgttgccg attccgcgtt ccgtaatctg
     7201 ttcgccaagc cacatcagca ataatgtgcc ggtgaccagg gtcatagtcg taagcagttc
     7261 aaatccgaaa cccgggatac gtacaacatc tgccgggaaa taaagctgtc cgctttcgag
     7321 agcacgtgcg atcaggaatc cctgaaaagc gcacagcgcc acggtcagat aacgggtgta
     7381 ctgcgtaatc ttggcccgtc cggcttcgcc ttcgcgaacc agctttccaa gagcaggaac
     7441 aaccgcagtc atcagctgaa tgataatcga agcgctgatg tacggcatga taccgagcgc
     7501 cccgacagca cagtttgcca ctgcgccgcc gctgaacaga ttaaacatat caagaaaccc
     7561 ggtgccttgc ccggcagagg atttaatgta gctgctgatc atgctgaaat ccacacccgc
     7621 tatcggaatg tcggaaacca gacggcacaa aaagatcacg cctaaagtaa aaagtatccg
     7681 ctggcgtaat tcctgaatct tgaagctgtt gacgaaggcg gaaagcatcg tttaccccgt
     7741 taaatttatg ggtcagacta tttcgcaagt accgccggca gcttcgattt tttctttggc
     7801 cgaagcgctg aatgcacagg ctttgaccga gagttttttc gaaatttcgc cgttaccaag
     7861 cactttaaca ccgtcccact gaccattgac cagtttgacc tgacgcaaca agtccaatgt
     7921 gaccaccgtg ccggcttcaa aggcattcag tgccgcaacg tttaccggag cgagcacctt
     7981 gcgggtgtag ttactgaagc cgcgcttagg aatacggcgg gccatcggca tctgaccacc
     8041 ttcgaaaagc ggcttatgct tatgtccgga acgggcatat gcacctttgt gtccgcgtcc
     8101 cgaggtcttc cctttgccgg aagcccggcc gcgaccgacg cgcattttcc ggtgtgtaga
     8161 accttttgca ggctgaagtg aatggagttc catttttaaa aacctcttaa tttattttcc
     8221 gcgggccgca agcgccgctt cgcgtgtacg cagagtggca agggcggcca tagtggcttt
     8281 ggttacgttc aatttattgc tgctgccgag ggattttgcg agaacatcgc gcacaccggc
     8341 aagttcgagt actgcacggc aggatccgcc ggcaattaca cccgtacctt cagacgccgg
     8401 acgcaaaaga acctgcgcgc cgccgaattt acctgttgtt tcatgcggaa tcgtcgttcc
     8461 gcgcattaca accggtgtca tggcccgttt ggccgcctcg ccgcctttgc gaatcgcatc
     8521 ggccacttcg ttggctttgc cgaggccgta accgacccgt ccggctccat cgccgacgac
     8581 aaccagcgcg ccgaagctga aacggcgtcc accttttacc acttttgaat tgcggttgat
     8641 gtgaacgacc cgttcttcaa actccgactt cacttttttt tctgctgatt tttcaatcac
     8701 ttcgagcaac tcctcattta cataaaacta aaactgaagg ccggcttcac gggcactgtc
     8761 agccagtgca cgcagacgcg atccgtaggc aaagccgccg cggtcgaaaa caaccttgga
     8821 gacgccctgt tctttggcta ctgcggccgc tttggttccc agcgctttgg cagtatccac
     8881 tgtgcacttt tcgcccagcg atgaaactga acaaagagtt ttctgcgcgg catcgtcgat
     8941 gacctgcacg taaatataac ggttggagac gtaaacagcc atgcgcgggc gctcggcggt
     9001 cccctgtacc ttgttccgca aacgaagatg tctgcgcttc cgataatctg atttgttttt
     9061 gaccttcatg attaagccac tgccttacct tctttgcgcc gaacctgttc acctttgtaa
     9121 cgcacccctt tacccttgta gggttctgcg ggagcaaatg cgcgaatgcg cgaagcaacc
     9181 agaccaacca gagccttttc gtgcccagat actttcacat cggttgcccc ggcgacttcg
     9241 actttgatgc cttcgggaat ttcatatctg atgtcgtgcg agaaaccgag cgagagaacc
     9301 aggatttttc cctgcaactg cgctttgtaa ccgacgccct gaatttcaag ttctttggcg
     9361 taaccgcttt gcacgccgag aatcatattc ttgatcgtgt tgcggattgt tccgtgcagc
     9421 gcacgggctt ctttagtttc atttttgcgt ccgaccgtta cccggccgcc ctcaactgcc
     9481 accgtaatca gcgcaggaat ctgcagagtg ctctgccctt tggcgccttt aaccgtgacg
     9541 acattcccgc tcagcgtcac ttccacgccg ccggaaaccg ttacaggcat ttttcctatt
     9601 ctagacatcg ttacaacctc aatttgaaat taccacacat aacaaagtac ttcgccgccc
     9661 acatgggctt tgcgagcttc tttatcgctg agcataccgg aagaggtact cagaatcgcc
     9721 agaccgatac cgccgagtac acgcggaaca tcatccgcca tcacataatg gtgacagctc
     9781 ggacggctga cgcgacgcaa cccctgaata accggctttt gatcgtcctg atatttgaga
     9841 tggagaacca gcgtcggttt gccttccacc ttctcggtgg tgtaatcctt gatgtaccct
     9901 tcctgcttaa ggatgcgggc cagttcgctc ttcatcttgg aatgcggcat ctgaaccttg
     9961 gtatgcgcag ccatattggc attgcggata cgagtcagca tatcagaaat aggatcgctc
    10021 aatatcattg ctgttcacct ttgtttttgg cggtttcaaa cggcagcccg atgaggctga
    10081 gcagttcata ggcttccgca ttggttttag ccgtcgtgac aaatgtcaca tccatgccgt
    10141 gtaccttgcg gacatggtcc ggattaatct ccgggaaaat ggtctgctct ttgaggccga
    10201 gagagtagtt cccgaaacca tcgaacgatt tgggcgggat cccgcgaaag tcacggatac
    10261 gcggcaacgc aatattgatc aggcgatcaa gaaattcata catccggtcg ccgcgcaacg
    10321 tcaccttgcc ggccagagaa accccctcgc gcagcttaaa gttagatacg ctcttccggg
    10381 ctttgatcgt taccggatgc tgtccggtga ttttggccag atcatcaagc accgcctgca
    10441 gcgcatcacg gtcatgctgc atgccgacgg atacgttaat at
//
LOCUS       DAOPZO010000063        10459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_7381_length_10459_cov_8.901865, whole genome shotgun sequence.
ACCESSION   DAOPZO010000063 DAOPZO010000000
VERSION     DAOPZO010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 10459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10459
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7381_length_10459_cov_8.901865"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..229)
                     /locus_tag="PKI68_05425"
     CDS             complement(32..229)
                     /locus_tag="PKI68_05425"
                     /inference="COORDINATES: protein motif:HMM:NF016028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HNX53165.1"
                     /translation="MEDRLIKMEMLYSDQSRMLEDLSQEMYQQQVEIKRLTARIKLLE
                     EKTASISESNNIGGHERPPHY"
     gene            296..1114
                     /locus_tag="PKI68_05430"
     CDS             296..1114
                     /locus_tag="PKI68_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNX53166.1"
                     /translation="MWKKVSKVLKPGMLCGMKILFTGDMVGSAGRQIFQRITERLRAE
                     NRIDLVIANAENAAGGRGPSPEIVEALFVAGAAALTLGDHAWDDRNLAPLLGTEPRLV
                     RPANFPPAAAGRGMTTIETDEGPICIISLIGRVFMDPADCPFRTADRLIGMAQAKTIL
                     IDFHAEATSEKIALGRYLDGRITALVGTHTHVQTSDEAILPKGTAYITDLGMTGPKDS
                     VIGREVEPVIQKFITGMPQKFDTADKDPALEGVILDIDIKTGKARSIERIRERA"
     gene            1114..2379
                     /gene="xseA"
                     /locus_tag="PKI68_05435"
     CDS             1114..2379
                     /gene="xseA"
                     /locus_tag="PKI68_05435"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNX53167.1"
                     /translation="MDDKRKIYSVTELNRTARFALENGIGEVWVEGEISRLTRHASGH
                     WYFTLKDADASVACAMFKQNNAAATFAPKDGLQVRVLGQASLYEPRGSYQLIIRKIEE
                     AGKGSLQEQFEKLKAKLAAEGLFDAARKKPLPLLPQKIGVVTSPTGAAIRDIIHVLTR
                     RFPTIEILLAPVTVQGEGAAAKIAAAIDYLNTRDDIDLMIVGRGGGSLEDLWAFNEEA
                     VARAIAASRIPVISAVGHEIDFTISDFVADVRAPTPSAAAELAVPVKTELETQLTRLA
                     ARLGGSLQNQVYVQRERIPGFRQTMIHALTDGLRLRQQKVDEAGRFLAECLKNAVAAQ
                     KQRLPVLQQTMTHRLESAVAERKQNLRRIEVQLRALSPLAVLDRGYSLTQTADGCVVR
                     DTAQIKPGDLLKTRLSKGTVISEVKEKEA"
     gene            2382..2612
                     /locus_tag="PKI68_05440"
     CDS             2382..2612
                     /locus_tag="PKI68_05440"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNX53168.1"
                     /translation="MAEKTVDFEKSLERLEAIVEEMESGDLSLEQMIKHFEEGSKLVT
                     LCSGKLNEVEQKIEKLVKKGGTLTAEPFKPEE"
     gene            complement(2664..3101)
                     /locus_tag="PKI68_05445"
     CDS             complement(2664..3101)
                     /locus_tag="PKI68_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNX53169.1"
                     /translation="MANVGKLLETKGKEIISVSPDTVIRDAICKMAEKSAGTALVMDG
                     NKLVGIFSERDIIRKVYLKDKCGKATKVSEIMSTGLTTVKPDTTLESCMEMMTEKRIR
                     HLPVLSGDQVVGVISIGDIVKFMVQEKEFVIKNLQSYISGPGM"
     gene            complement(3171..4037)
                     /gene="dapA"
                     /locus_tag="PKI68_05450"
     CDS             complement(3171..4037)
                     /gene="dapA"
                     /locus_tag="PKI68_05450"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNX53170.1"
                     /translation="MLQGAHTAIVTPFNKDGSVDYVRYRELIEFQIENGIDGIVPVGT
                     TGESPTLNNDEHMKVIEETVKTVHRRALVIAGTGANSTSEAVELTTLAKELGVDATLQ
                     VTPYYNKPNQEGLYRHFSTVADIGLPVVLYNVPGRTSREIAIETVARLAAHPNVVAVK
                     EAGGKVERVKQTLDVCDLEVLSGDDALALPMMRDGAIGVISVASNVVPGVVASLIHTA
                     LKGNWDAAQLLHNRYVKLFDAMFIDTNPIPVKAALAMMGKIEEVYRLPLCEMSATDKE
                     TLRNVMTSSGVL"
     gene            complement(4068..>5343)
                     /locus_tag="PKI68_05455"
     CDS             complement(4068..>5343)
                     /locus_tag="PKI68_05455"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNX53171.1"
                     /translation="EKMMPLKKCPLNREQLEALTKTNPTPFHLYDETAIRANARALKT
                     AFSWCPGFKEYFAVKAAPNPYLMKILKAEGFGADCSSLPELMLAESVGLSGEEIIFTS
                     NDTPANEYVKAVQLGAIINLDDISHIEFLDQCTGLPELVCFRYNPGPLKGGNAIIGHP
                     EEAKYGFTRPQLFEGYRILRDKGVKRFGLHTMVASNELDAVYFIETAHLLFDLVAELS
                     AGLGIKFEFVNIGGGIGIPYKPEQNPVDLKVVGEGIRKLYAEKIEKTGLGPLDVRMEC
                     GRMVTGPYGWLVSRVLHKKNTYKQYVGLDACMANLMRPALYGAYHHITVPGKENLPHD
                     FVCDVTGSLCENNDKFAINRALPPVEIGDLVVIHDAGAHGHAMGFNYNGKLRSAELLL
                     RENGEVVQIRRAETVQDYFATLDFAGLNKFNV"
     assembly_gap    5344..5443
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5444..6897)
                     /locus_tag="PKI68_05460"
     CDS             complement(<5444..6897)
                     /locus_tag="PKI68_05460"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HNX53172.1"
                     /translation="MAVRNLIILNNPAHWTFDIEEVEVVSAKAYLTESRYAEMKNARV
                     FNLCRSYRYQSVGYYVSLLAAARDHRAIPSVTTMQDFRSQTIIRTIAEDIDDLIQKTF
                     AKVTAKEFTLYIYFGQTVLPEHRYVGRSLYNLFQAPLLKVSFEFTTKWLIQQITPLSL
                     GLIPESDQEHIADFARSYFSRKRFQKSQIQQYAFDLAILVNPEEKNAPSCKKALKKFE
                     EAADELNVYTEFITKEDYSRLPEFDALFIRETTSVNHHTYRFSRKAFAEGLVVIDDPF
                     SILRCANKVYLAERLAQAKVPAPRTVILQKESLKSSPASQGITFPCVLKQPDSAFSHG
                     VTKADNETEFREKLDKLFEKSDLIIGQEFMQSDFDWRIGILDRKPLYACKYYMARGHW
                     QICNWAGATNSRFGKAETMRVEDAPPAVVQTALKAANLIGDGLYGVDLKEVDGKVYVI
                     EVNDNPSIDAGVEDIVLKDDLYLTVIRSIINRIDG"
     gene            complement(6884..7372)
                     /locus_tag="PKI68_05465"
     CDS             complement(6884..7372)
                     /locus_tag="PKI68_05465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNX53173.1"
                     /translation="MSTIHIRAATLEDLPAIEVIEKSCFHADLRVSPRALRYSFKSPI
                     QSVWVAVGGQNETIGAMVLYHHPRSIRIFSIAVLPAFRGSGAGRQMMEKALSMARHAG
                     CDAVTLEAELSERKLISWYENFGFEIKKTLIDYHSSGRHAVRMRLALKPALKGGRVCG
                     RS"
     gene            7362..7640
                     /locus_tag="PKI68_05470"
     CDS             7362..7640
                     /locus_tag="PKI68_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53174.1"
                     /translation="MVLMRLSCNLKPAYYHRLELHTSRNHQENVFHRSALSGRSGIAA
                     QTRLRNDDSTCFKPWRYIAPLIELNDLLKQRPEPAGLRETILKAHREK"
     gene            complement(7692..7991)
                     /locus_tag="PKI68_05475"
     CDS             complement(7692..7991)
                     /locus_tag="PKI68_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53175.1"
                     /translation="MKKWVVLAVAVMMVAGVQAKEKGKGKGPGPSSDITKAAFIAAEQ
                     KKAEVAGTAFDPAAAEAAFAAQDKNGDGVLTADEQTPAAGKHAKKGAKGSKTLVQ"
     gene            complement(8186..9388)
                     /locus_tag="PKI68_05480"
     CDS             complement(8186..9388)
                     /locus_tag="PKI68_05480"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNX53176.1"
                     /translation="MKVVLAYSGGLDTTVLLTWLQEQFNAEVICYCSNVGQEEELDGL
                     EAKALKHGAVKCIVDDVCEEFAKDFVFPMMQANAIYEGTYLLGTSIARPLIAKRMMDI
                     AIAEGAEAICHGATGKGNDQVRFELTAYSMKPDIKIIAPWRMPEVFKFKGRPDMIKYL
                     EEHGIPCSVSKSKPYSTDRNLLHISFEGGILEDPWNEPDENMWCMTKPLSQAADKPEY
                     VEISFEKGIPVAVNGEKLSPAALIRKLNAIGCEHGIGRIDIVENRFTGMKDRGIYETP
                     GGTILHHAHRAMESITMDREVMHLRDSLIPKYATLVYNGFWFSPEREMLQAAILESQK
                     TVTGDVRVKLFKGGVHVCGRRSPYSLYSSKIVSFDEAGGYDQTDATGFIKLNALRLRV
                     RAAMQKKK"
     gene            complement(9411..9743)
                     /locus_tag="PKI68_05485"
     CDS             complement(9411..9743)
                     /locus_tag="PKI68_05485"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNX53177.1"
                     /translation="MPFSPDDLIRHILDEIRFLTGESSVLSEDGFMRDEKAQRAFARS
                     FEIIGEAAKKMPDDFKALHPEVNWKAMARMRDKLIHHYFGVDYFLVWNTVRENLPELG
                     RQLEKLLI"
     gene            complement(9727..10029)
                     /locus_tag="PKI68_05490"
     CDS             complement(9727..10029)
                     /locus_tag="PKI68_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNX53178.1"
                     /translation="MSRKESILNLLSSHADALHRMGVTAIGLFGSVARGDDTASSDVD
                     ILVEFEKQGNTFRNFNALCDFLERHLGDRYDLVTRNSLSPYMRPHILDEVEYAVLA"
     gene            complement(10106..>10459)
                     /locus_tag="PKI68_05495"
     CDS             complement(10106..>10459)
                     /locus_tag="PKI68_05495"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HNX53179.1"
                     /translation="MAYDLTGKIKVVKEAQTFGTKGFTKREFVVTVEDGKYPQDIALE
                     CVQDKVSMLDGLKEGQLVTVTFDIRGREYNGRYFNNLQAWRIKAENGGAADDDRPPVP
                     TDAEAPGNFDDDIPF"
BASE COUNT         2341 a         2971 c         2708 g         2339 t
ORIGIN      
        1 gcggaaatga ttgggagatg ttgccgccgg atcaataatg cggcgggcgt tcatgaccgc
       61 cgatattgtt cgattcggaa atcgatgccg ttttttcttc cagcagttta atccgggcgg
      121 tcaaccgttt gatttcgacc tgctgctgat acatctcctg actgagatct tccagcattc
      181 tgctctgatc cgaataaagc atctccatct tgatcagccg atcttccata aaagtctcct
      241 tgaaggctcc accaaaccag aaagcggtcc ggcgcgcaat gttaccggtg ccaatgtgtg
      301 gaaaaaagtt tccaaggttc tcaaaccggg tatgctgtgc ggcatgaaaa ttctctttac
      361 aggcgatatg gtcggcagcg ccggccggca gattttccag cggatcaccg agaggctccg
      421 cgcggaaaac cggatcgacc tcgtcatcgc caatgccgaa aacgccgccg gcgggcgcgg
      481 cccgtcgccg gaaatcgtcg aagctctttt cgtcgccgga gccgccgcgc tgacgctcgg
      541 cgaccacgcc tgggacgata gaaatctcgc ccccctgctt ggcactgaac ctcgcctggt
      601 ccgcccggct aactttccgc ccgccgcagc gggccgcggc atgacaacca tcgaaacaga
      661 cgaaggcccc atctgcatca tttctctgat cggccgcgtt ttcatggatc cggccgactg
      721 cccgttccgc accgccgacc ggctgatcgg catggcacag gccaaaacca tcctgatcga
      781 tttccacgcc gaggcgacgt cggaaaaaat cgcgctgggc cgttatcttg acggacgcat
      841 caccgccctg gtcggcacgc atactcacgt ccagacctcg gacgaggcca ttctgccgaa
      901 aggcacggcc tatatcaccg atctcggcat gaccggcccg aaagattccg ttatcggccg
      961 cgaagtcgag ccggtcattc aaaaattcat caccggtatg ccgcagaagt ttgatacggc
     1021 ggataaagat ccggcactcg aaggcgttat tctggatatc gacatcaaaa ccggtaaagc
     1081 ccgcagcatt gagcggatca gagaacgtgc ctgatggacg acaaacgcaa aatctacagc
     1141 gttaccgagc tcaaccgcac cgcgcgtttt gcactcgaaa acggcatcgg cgaggtctgg
     1201 gtcgaaggcg aaatttcgcg cctgacccgc cacgccagcg gccactggta ttttacgctc
     1261 aaagacgccg atgcttccgt cgcctgcgcc atgttcaaac agaacaacgc cgcagccacg
     1321 ttcgcgccga aagacggtct tcaggtccgc gtgctcggac aggccagcct ttatgaaccg
     1381 cgcggaagct accagctcat catccgcaag attgaagagg ccggcaaagg ctcgcttcag
     1441 gagcagtttg aaaaactgaa agccaaactc gccgccgaag ggctgttcga tgccgcccgc
     1501 aaaaaaccgc tgccgctgct gccgcagaaa atcggcgtcg tgacctcgcc caccggcgcc
     1561 gccatccgcg acattatcca tgtgctgacg cgccgctttc cgacaattga aatcctgctc
     1621 gcgccggtca ccgtacaggg cgaaggcgcg gcggcgaaaa ttgccgccgc gattgattat
     1681 ctgaataccc gcgacgatat tgacctgatg atcgtcggac gcggcggcgg cagcctcgaa
     1741 gacctgtggg cgttcaacga ggaagcggtc gcccgcgcca ttgccgcttc gcggattccc
     1801 gtaatctcgg cagtcggcca cgaaattgat tttacgatca gcgatttcgt tgccgatgta
     1861 cgtgcgccga cgccgtccgc cgccgcagaa ctggccgtgc cggtcaaaac cgaattggaa
     1921 acgcaactta cgcggctggc cgcgcggctc ggcggttcac tgcaaaacca ggtgtatgtt
     1981 cagcgcgagc ggattcccgg cttccggcaa acgatgattc atgcgctgac agacgggctg
     2041 cggcttcggc agcaaaaagt ggatgaagcc ggccggtttc tcgcggagtg tttgaaaaac
     2101 gcagtggcgg ctcagaagca gcgccttccg gttttacaac aaaccatgac tcaccggctg
     2161 gagagcgcgg ttgcggagcg taaacaaaat ctgcgccgaa tcgaagtgca gctgcgtgca
     2221 ttaagtccgc tggccgtgct ggatcggggc tacagtctga cgcaaactgc cgatggctgt
     2281 gtcgtgcgcg atacagcgca gatcaaaccg ggcgatctgc tgaaaacccg cctttcaaaa
     2341 ggaactgtca tttcagaagt caaagagaag gaggcatgat tatggccgaa aaaactgtcg
     2401 attttgaaaa atctctggag cggctcgaag ccattgttga agagatggag agcggcgatc
     2461 tttcgctcga acagatgatt aagcatttcg aggaaggctc gaagctggtg acgctctgct
     2521 ccggcaagct caacgaggtg gagcagaaga ttgaaaagct ggtgaaaaaa ggcggcacgt
     2581 tgaccgcaga gcctttcaaa ccggaggaat aaaaaaggtt tccggaagcc ggaaaccttt
     2641 tcattgaacc tcaggaaccg tttctacatg ccggggccgg agatgtaact ctgaagattt
     2701 ttgatcacga attctttttc ctgcaccatg aatttgacga tgtcaccgat tgaaataaca
     2761 ccgacgacct gatcgccgct gagcaccggc aggtgacgaa tgcgtttttc agtcatcatc
     2821 tccatacagc tctcaagtgt tgtatccggc ttaacggtgg tgaggccggt agacatgatt
     2881 tcgctcacct tggtggcttt accgcatttg tctttcagat agactttgcg gataatatcg
     2941 cgctcggaaa aaatgccgac cagtttgttg ccgtccataa ccagagccgt gccggccgat
     3001 ttctcagcca ttttacagat tgcatcgcgg atgacggtgt ccggcgatac agaaatgatt
     3061 tctttgcctt ttgtttcaag cagtttacca acgttggcca tatgattgtc ctttctgttt
     3121 aaacccgctg ccccgttcag ccccgcggaa agctccgagc agaggctgga ttacaataca
     3181 cctgatgacg tcattacgtt ccgcagagtt tctttatccg ttgcgctcat ttcgcagagc
     3241 ggcagacgat agacctcttc gatcttaccc atcatagcca gcgcggcctt gaccggaatc
     3301 gggttggtat cgatgaacat tgcgtcaaaa agttttacat accggttgtg cagcagctgg
     3361 gcggcatccc aattgccttt cagcgcggta tgaataagcg aagccaccac acccggaact
     3421 acattcgagg ccacgctgat caccccgatc gcgccgtcgc gcatcatcgg cagcgccagt
     3481 gcgtcgtcgc cggaaagcac ttcgagatcg cagacatcca gcgtctgttt cacgcgctcg
     3541 actttgccgc ccgcttcttt gaccgccact acattcggat gagcagccag ccgagcgacg
     3601 gtttcgattg cgatttcgcg gctggtgcgg ccgggaacat tataaagcac aaccggcagt
     3661 ccgatatccg ccaccgttga aaaatggcga tagagccctt cctgattggg cttgttgtaa
     3721 tacggcgtca cctgcagcgt agcgtcaacg ccgagttcct tggcaagcgt tgtcagttcg
     3781 accgcctcgg aagttgaatt agcgcccgtt ccggcaataa ccagcgcacg gcggtgaacc
     3841 gttttgacgg tttcttcaat caccttcatg tgctcgtcgt tgttgagcgt aggcgactcg
     3901 cccgttgtgc cgaccggcac aatgccgtcg ataccgttct cgatctgaaa ctcgatcagt
     3961 tcgcgatacc ggacgtagtc taccgatccg tctttattaa acggtgtgac aatcgctgtg
     4021 tgggctcctt gcaacatggc tttctcctgt tggcggcaga ctatgcccta tacgttgaat
     4081 ttattcaaac ctgcaaaatc gagggtggcg aaatagtctt gaaccgtttc ggcacgccgg
     4141 atctgcacaa cctcgccgtt ttcacggagc agcagctcgg ccgaacgcag tttgccgtta
     4201 taattaaagc ccatggcgtg accgtgcgcg cccgcatcgt gaatcacgac caggtcaccg
     4261 atttcaaccg gcggaagcgc gcggttgatg gcgaacttat cattgttttc gcacagcgaa
     4321 ccggtcacat cgcaaacgaa gtcatgcggc aggttttctt tccccggcac agtaatgtgg
     4381 tgatacgcgc cataaagcgc cgggcgcatc agattggcca tacaggcgtc gaggccgaca
     4441 tactgcttgt aggtgttttt tttatgcagc acgcgactga ccagccagcc gtacgggccg
     4501 gtcaccatcc ggccgcattc catgcgtaca tcgagcggac ccaggccggt tttttcaatc
     4561 ttctcggcat agagcttacg gataccttcc ccgaccacct tcaggtcaac cggattctgt
     4621 tccggtttgt aaggaatgcc gatgccgccg ccgatattga cgaattcaaa tttgattccc
     4681 agtccggcgg aaagttccgc caccagatcg aacaggaggt gcgcggtttc gataaaataa
     4741 accgcgtcga gttcgttcga cgccaccatc gtatgcagac cgaagcgttt cacgcctttg
     4801 tcgcgcagga tgcggtagcc ttcgaaaagc tgcggacggg tgaatccgta ctttgcttct
     4861 tccggatggc cgatgatcgc attgccgccc ttgagaggcc cggggttgta gcggaaacag
     4921 accagttcgg gcagccccgt acactgatcg agaaattcaa tgtggctgat gtcgtcgaga
     4981 ttgataatcg caccgagctg cacggccttc acatactcgt tggcaggcgt atcattcgag
     5041 gtgaaaatga tttcttcacc ggaaagaccg acggactcgg caagcatcag ctcgggaaga
     5101 gaagaacagt ccgcgccgaa cccttcagcc ttcaggattt tcatcagata cggattgggc
     5161 gcggctttaa cggcgaaata ttctttaaag ccggggcacc aggaaaatgc cgtcttcaag
     5221 gcgcgggcgt tagcgcgaat ggcggtttcg tcatacagat ggaacggcgt cggatttgtt
     5281 ttggtcagag cttcgagctg ctcacgattt aaaggacatt ttttcagggg catcattttc
     5341 tccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccgtcaa tgcggttgat
     5461 aatagaacgg ataacggtca gatagagatc gtctttaaga acaatatctt cgactccggc
     5521 atcaatgctt ggattgtcat ttacttcgat gacatacact ttgccatcaa cctctttcag
     5581 gtctacgccg taaaggccgt caccgatcag gttggccgct ttaagcgcag tctgcacaac
     5641 ggccggcggc gcatcttcca cccgcatggt ctcagccttg ccgaagcggc tgttcgtcgc
     5701 gccggcccag ttgcaaatct gccagtggcc gcgcgccata tagtatttgc aggcgtagag
     5761 cggtttgcgg tcaaggatac cgatgcgcca gtcgaagtcg gactgcatga attcctgccc
     5821 gatgatgagg tcggatttct cgaacagttt atccagcttt tcgcggaact ccgtctcgtt
     5881 atccgccttg gtcacgccgt gcgaaaaggc gctgtcgggc tgcttgagca cgcagggaaa
     5941 ggtgatcccc tggctggccg ggctggattt gagggattcc ttctgcagaa tcacggtgcg
     6001 cggcgcgggc actttggcct gagccagacg ctccgccaga taaaccttgt tggcacagcg
     6061 cagaatcgaa aacgggtcgt cgatcaccac cagcccttcg gcaaaggcct tgcgcgagaa
     6121 gcggtaggta tgatgattca ccgacgtcgt ttcgcgaata aacagcgcat caaactccgg
     6181 caggcggctg taatcctctt tcgttataaa ctcggtatag acgttcagct cgtcggccgc
     6241 ttcctcgaac ttcttcagcg ccttcttgca cgacggcgcg tttttctctt ccgggttcac
     6301 cagaatggcg agatcaaagg catattgctg gatctggctt ttctgaaacc gcttgcgcga
     6361 aaaatagctg cgggcaaaat ccgcgatatg ctcctgatca ctttccggaa tcaaccccag
     6421 cgaaagcggc gtaatctgct gaatcagcca tttcgtggtg aactcaaagc tgactttcag
     6481 cagcggcgcc tgaaaaagat tatagagtga acgtccgaca taacggtgtt ccggcagaac
     6541 ggtctgcccg aaatagatat agagcgtgaa ctcttttgcg gtcactttgg cgaacgtctt
     6601 ctgaatcaga tcatcaatat cctcggcaat cgtgcggatg atggtctgag aacggaaatc
     6661 ctgcatggtg gtcacgctgg gaatcgcacg atggtcgcgg gcggcggcca gcagagaaac
     6721 gtaatagccg acggattgat agcggtagga acggcacaga ttgaaaacac gcgcattctt
     6781 catttccgca tagcggcttt cggtcagata agctttggcg gaaacgacct ccacttcctc
     6841 aatgtcaaag gtccagtgcg ccggattgtt gagaataatc aggttacgaa cggccacaaa
     6901 cgcgccctcc tttaagcgcc ggcttcaaag ccagccgcat gcggacggca tggcggcctg
     6961 aagagtggta atctatcaac gtctttttga tttcaaaacc gaagttctca taccagctga
     7021 tgagcttgcg ttcactaagc tccgcttcaa gcgttactgc gtcgcatcct gcgtgacgtg
     7081 ccatgctcaa cgccttctcc atcatctgcc gacccgcgcc gctgccgcga aaggcaggca
     7141 gaaccgcgat tgagaaaata cggatggaac gagggtgatg atagagcacc atggcgccga
     7201 tggtttcgtt ctgcccgcca acagccaccc agacactctg tatcgggctc ttaaagctgt
     7261 accggagggc acgcgggctg acacgcaggt cggcatgaaa acagcttttc tctattacct
     7321 caatcgccgg aaggtcttcc agtgtcgcgg cccggatatg gatggtgctc atgcgccttt
     7381 cttgtaattt gaagcccgct tactaccatc gtttagaact gcacaccagc aggaatcatc
     7441 aggaaaatgt tttccatcgt tccgcacttt ccggccggtc gggcatcgcc gcccaaaccc
     7501 gccttcggaa cgacgattca acatgtttta agccatggag atacatcgct cctctgattg
     7561 aactgaacga tcttctgaag cagcgtccgg aacccgccgg gttgcgcgaa accatactga
     7621 aagctcatcg ggaaaaataa aaaaagccct gcggatttcc gcaggacttt ttcaacactt
     7681 cagcagccgg cttattgaac taatgttttg ctgcctttgg cgcctttctt ggcatgtttg
     7741 ccggcagcag gagtctgttc atcggctgtc agaacgccgt cgccgttctt gtcctgagcc
     7801 gcgaatgcgg cctcggcagc agccggatca aatgcggttc ctgcaacttc agcctttttc
     7861 tgctccgctg caataaatgc cgccttggtt atatcgctac ttggaccggg acccttgcct
     7921 ttaccttttt cttttgcctg aactccggca accatcatca ccgcaactgc caacacaacc
     7981 catttcttca taccagtctc cttggaacag ctccctgcgc cggacaaccg gttccccgca
     8041 cggggaactc cgtaatttga ccggacgcgt acagtaaaat aaaacgtcac aaccctcgat
     8101 aaattgcacc ggcaagagaa aaataccgca gcaaaaaagg gttccggaga ccggaaccct
     8161 ttctctcagc cggtaaaccc gggccttatt tcttcttctg catcgccgca cggacgcgca
     8221 gccgcagggc attgagctta ataaagcccg tcgcgtcagt ctggtcgtag ccgcctgctt
     8281 cgtcaaagct gacgatcttc gagctgtaga gcgaatacgg cgagcggcgc ccgcaaacat
     8341 gcaccccgcc tttgaacagc ttaacgcgga catcgccggt aaccgttttc tggctttcca
     8401 gaatcgccgc ctggagcatt tcgcgttccg gtgaaaacca gaagccgttg taaaccagcg
     8461 tggcatattt cggaatcagc gagtcgcgca ggtgcatcac ttcgcggtcc attgtgatcg
     8521 actccatcgc gcggtgcgcg tggtgcagga tcgttccgcc tggagtttca tagataccgc
     8581 ggtctttcat gccggtgaag cggttttcga cgatatcgat gcgtccgatg ccgtgttcgc
     8641 agccaatagc gttgagtttg cggatcagcg cggcaggcga aagcttttcg ccgttaacag
     8701 cgaccggaat acctttttca aaactgattt ccacatactc cggtttgtcg gccgcctgcg
     8761 acagcggctt ggtcatgcac cacatatttt catccggctc gttccacgga tcttccagaa
     8821 tgccgccttc gaagctgata tgcagcaaat tgcggtcggt gctgtacggt tttgatttcg
     8881 aaaccgaaca gggaatgccg tgttcctcga gatatttgat catgtcgggg cggcccttaa
     8941 atttgaagac ctccggcatg cgccacggag cgatgatctt aatgtcgggc ttcatcgaat
     9001 aggcggtcag ctcgaaacgg acctgatcat ttcccttgcc ggttgcgccg tgacaaatgg
     9061 cttccgcgcc ttcggcgatc gcgatatcca tcatgcgctt ggcgatcagc gggcgggcaa
     9121 tcgaagtgcc gagcagatag gtgccttcgt aaatcgcgtt cgcctgcatc atcggaaaaa
     9181 caaaatcctt cgcgaactct tcgcagacgt catccacaat gcatttgacc gcgccgtgct
     9241 tgagagcttt cgcttccagc ccgtcaagtt cctcctcctg cccgacgttc gagcagtagc
     9301 agatcacttc cgcgttaaat tgttcctgaa gccaggtcag aagcactgtc gtgtccagac
     9361 cgcctgaata tgccaataca actttcatca ttttctccaa ttgaactgtt ctatattaaa
     9421 agtttttcca actgccgtcc aagttccgga agattctctc gaacggtgtt ccagacaaga
     9481 aaatagtcca ccccgaaata atgatggatc agcttatccc gcatccgggc catcgctttc
     9541 cagttcacct ccggatggag cgccttaaaa tcgtccggca tcttcttggc cgcttcgccg
     9601 ataatttcaa agctgcgtgc aaaagcccgt tgagcctttt catcccgcat aaaaccgtct
     9661 tcagacagga cggatgactc accggtcagg aaacggattt catcgaggat atgccggatc
     9721 agatcgtcag gcgagaacgg catattcaac ctcatcaaga atgtgcggcc tcatgtaggg
     9781 actcaggctg ttacgggtca ccagatcgta acggtcaccc agatgacgct ccagaaaatc
     9841 acacagcgca ttgaaattcc ggaatgtatt cccctgtttt tcaaactcaa cgaggatatc
     9901 cacatcactg gaagccgtat catcgccacg cgccacggaa ccgaaaagac cgatagccgt
     9961 cacacccata cgatgcaatg catccgcgtg ggaactcagc agattcaata tggattcttt
    10021 gcggctcaca gcgtttctat acggttttca gaacgaggac tcaacctcat ccttccatta
    10081 ttccgcccgc aacacccttc cattctcaga acggaatatc gtcgtcaaaa ttgccgggag
    10141 cctcggcgtc ggtcggaacc ggcggacggt catcgtcggc ggcaccgccg ttttcggcct
    10201 tgatgcgcca cgcctgcaga ttattgaaat agcgcccgtt gtattcgcgg ccgcgaatgt
    10261 cgaacgtcac cgttaccagc tgaccctctt tcagaccgtc gagcatcgaa actttatcct
    10321 gaacacactc cagcgcgata tcctgcggat acttaccgtc ctcgaccgtc accacaaact
    10381 cgcgcttcgt aaagcccttc gtcccgaagg tctgggcttc cttaacgacc ttaattttac
    10441 ctgtcaaatc atacgccat
//
LOCUS       DAOPZO010000064        10422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_7421_length_10422_cov_11.050545, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000064 DAOPZO010000000
VERSION     DAOPZO010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 10422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10422
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7421_length_10422_cov_11.050545"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PKI68_05500"
     CDS             complement(<1..756)
                     /locus_tag="PKI68_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNX53180.1"
                     /translation="MKKAAPVHILISSAGNAADIRYLCGAEPPEPAVLILNGARGTLI
                     VSEMEYSRMRRTVKRSIAVVHPELIGSSWKKGPADWIKKTVTGKKLTVASGFPFGLAE
                     KLKRAGRKLEISETPLCPQRERKTADEISAVKKAQRAAVAAMNAAIQQIASSQIGKDG
                     LLRCGRTVLTSEMVRATICRTLLDRDCVATETIVACGPHSADPHERGAGPLRAGQPIV
                     LDIFPRSTATGYWGDLTRTVCRGPAPAELKKMYN"
     gene            1046..2413
                     /gene="dnaA"
                     /locus_tag="PKI68_05505"
     CDS             1046..2413
                     /gene="dnaA"
                     /locus_tag="PKI68_05505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNX53181.1"
                     /translation="MRKDKDKLWEDACSHLQETLPKDIFEKWIAVIQCRSMEDTRAVL
                     VVGNDFYQSWLEEHYLPLIRKALGVVSGREMQIELTVDYSLITEQPAEKRDKTPVAAT
                     AKKPAEHNLNKNYTFDSFVVGPSNQFAHAASIAAAQAPSRAYNPLFIHGDVGLGKTHL
                     MQAIGNFIHSKNSRGKVCYATCESFTNEYIEALQTNTLAKFRKKYRTVDALLIDDIHF
                     LAGKDRMQEEFFHTFNAIFENHKQIVMTSDRPAGELSGLAPRLVSRFEWGLVVELESP
                     DIETRVAILRKKCEQLNLMIDQSLLFYIAERISSNIRRLEGALIRVASYVSLTGQSLD
                     RSKLEYLLRDTLDQEKKNAISIEGIQKAVAEHFDIRVSDLVGPKRPQNIAWPRQVAMY
                     LARTMTSQSFPVIGDAFSRNHATIVHACQLVEQRQTEDIKLRQTLSLLRQRVSHSSQK
                     AAQGN"
     gene            2643..3737
                     /gene="dnaN"
                     /locus_tag="PKI68_05510"
     CDS             2643..3737
                     /gene="dnaN"
                     /locus_tag="PKI68_05510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNX53182.1"
                     /translation="MKLTVEKDALLEALQKVQSIVGQRSTLPILSNVLLKAEGDTVSL
                     TTTDMEVCVKTGAKAEINEGGGTTLPARRFFNICRELPGGQVEIEVDAKDIATIRCGP
                     SFFKLVGLPEEEFPPLPEFEESAVYTIDQNSFKEMLQKVVYASSTDETRYILNGALLS
                     FKDEKLTVVATDGRRLALVEQEVEFSEDAQANLVVPTKTLNELIKTLGDEGVLRIRVS
                     ATQVAFDFDRILIISKLIEGTYPNFQQVIPAQCEERIAIDRETILNAVRRVSLLTDDQ
                     TASIRLNFGKNKLELVTNSPDIGEARETIPVKYEGKEISIAFNPGFLMAPLRHLDSDE
                     IFFELSDELSPGVIKTGVPFLYVLMPIRIS"
     gene            complement(3748..3821)
                     /locus_tag="PKI68_05515"
     tRNA            complement(3748..3821)
                     /locus_tag="PKI68_05515"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3787..3789),aa:Gln,seq:ctg)
     gene            3882..4121
                     /locus_tag="PKI68_05520"
     CDS             3882..4121
                     /locus_tag="PKI68_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53183.1"
                     /translation="MREGGQSRVIGLLGVGFDHADGQIRITQADDYQVLMGSGETHEE
                     LQKICGRIDECIKASGRALSDYTPEEFMEMVQELY"
     gene            4134..5339
                     /locus_tag="PKI68_05525"
     CDS             4134..5339
                     /locus_tag="PKI68_05525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX53184.1"
                     /translation="MKILFLTNKLPHAEVAGGHRLIYQRIRYLSERGHRIGLLTFVQG
                     ETAAQIESLKPLLTELHTLPHPRRNIFIRALHDYLSLSRPAVFWKSYSRDMMKEVGQV
                     AEQGKYDLVIAEFSEMGQYLHKNPYLSAIHKVISCHRCVTASYEKYKSLGEVRLKLYL
                     KSIPQLRGLQKYEFNMYRSADRILVLTPQDRFTMQYYAQDLAISVAPAGVDIRYLQEH
                     PPVPKEPIVLITGFMSDPANEDSVEWFYHHVWPRLSERHPEVKFYIVGAGVRPSIKRL
                     AEKDCRIIVTGEVEDMRPYRNRARVFVSPVRLGSGMRTKVLEAMAGGVPVVSTSLGMA
                     GIDAQTGVNCLVADTPELFTQSVEWLLTDRALAARMVRNARELVAKKHSLESGMRRFE
                     NIITSIAEG"
     gene            5550..8741
                     /locus_tag="PKI68_05530"
     CDS             5550..8741
                     /locus_tag="PKI68_05530"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNX53185.1"
                     /translation="MALPFTQKILQDWAGWKSFRDGKLLFERGVVEKIEYNHPFISGE
                     LAVGMRGMRTKFEILPDGLVENHCPCRDNQERGLICAHLVALGLEAIRLYNDPLRQEK
                     ALKEKQRAARLEQLDESKYITRVPAGHPKAVSAVLRLQLEQNWREQSLGGRFPLRCFA
                     SYNGRDIPLNAVPADVPLSFTPRDENLLFVLEDICEGPARGRLVAGEADFINLLQLCI
                     NKPVFEEGETRPLTVLPETVGTILRMELDEATGELNLSMHVKSSGGSGSPLFIIGARE
                     GWIFEQNTFRKLDELLPAPLRSIYHNPTRIARPAVPHFLHHELPTLGNLIPVDTKVSP
                     DLFTLTPAEPRFRLVVSGSRASLSAKLYAEYGEIVLVAARDEAAGGFAIPSPDDILEY
                     RMRNPEAEKQALEKLATTGFRGHRGDLLTSIVGPREVLNFLGGSIPRLRRYGWRVELE
                     GRVAPFMDEAEFTTPVIRINEASGGGFFDVGYAYENSGGQSLSESEIQRALNMGEAFV
                     EKNGRTILLDIDAIETAREVFSDCAFGAGGAAGTFRMNDIYAAYVQASLHSLDGVDVE
                     ASPDWMKMAQTQNRAQKIEPEPLGEKLEATLRDYQKDGVYWLRFLERSGFSGLLADEM
                     GLGKTLQALVWLQLERHNEKARGKPALIVCPTSLVDNWAEEAEKFTPHLRVLKLHGAE
                     RHDHWEKVGESDLIITSYALLRRDIEKYLEHEFAVAILDEAQHIKNRSTQNAVAAKKI
                     RAMHKLVLTGTPIENSVADLWSIMDFLMPGYLSNHKAFREHYELPIGRGGPDGELAQL
                     KLRRKLHPFLLRRLKREVAKDLPEKIQRVAHSSLSKDQYMVYKELLESSKRKISDMVA
                     SQGFNRSRMQIFKTLLRLRQTCCHLDLLKLPGLQSEFPSAKMELFFELVDEAIDARHR
                     ILVFSQFTSMLAILRRELEARELKYCYLDGATKERQSIVRQFNTDRSIPVFLISLKAG
                     GSGLNLTGADMVIHYDPWWNPAVEDQATDRAHRIGQKNTVYSVKLITKDTVEEKVLQM
                     QQRKQGVISATLEKEGEFMQTLTWSDVQELLSL"
     gene            8757..10085
                     /locus_tag="PKI68_05535"
     CDS             8757..10085
                     /locus_tag="PKI68_05535"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX53186.1"
                     /translation="MTKKQIHPDDMSRWQLMFRALSHRNFRLFFMGQSISLLGTWMQM
                     TAVAWLVWRLEHNAFLLGLAGFASRIPALVMAPLAGVLVDRWNRYRLVIIAQVLAMIQ
                     ALLLAALLYSGHAAIWSVILLSLFLGFINALDMPARQAFLIQMLDRREDLTSAISLNS
                     AMVNGARLMGPVAAGLIIAAAGEGLCFLINGLSYIAVIAGLLMMRVSPHHYVRPETNI
                     AHNLKEGFRYAFGFPPIRALLLLIALVSLAGASYAQLLPVFAQQILHGGPRTQGLLAS
                     AAGLGALTGAVLLATRRSVRGLGRWIACAPVVFGLGLIGMGLSSSLWLTLAVMPVVGI
                     GMMAQMAATNTVLQTIVDDDKRGRVMSFYSMAFMGTVPLGSLLAGSLVHLIGAPWTVI
                     SGGLCCIAGAAVFATKLKNINRLIHPIYVRKGIIPEVAAGLQSASGVTQP"
     gene            complement(10144..>10422)
                     /locus_tag="PKI68_05540"
     CDS             complement(10144..>10422)
                     /locus_tag="PKI68_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNX53187.1"
                     /translation="NEIAQNIFRTGSSMEALQNFWQSLNKMIMNSRAQQAQQPSAMTA
                     ARQQQLGHGAPPVAEAAPQPIRHTGPETGRNDPCPCGSGKKFKKCCGQ"
BASE COUNT         2309 a         2922 c         2927 g         2264 t
ORIGIN      
        1 gttgtacatt ttcttcagtt ccgccggtgc cgggccgcgg caaaccgtac gcgtcaggtc
       61 gccccagtaa cccgtcgcgg tggagcgcgg aaaaatatcg agaacgatcg gctgtccagc
      121 gcgaagcggg ccggctccgc gttcgtgcgg gtcggcggag tgcggtccgc aggcgacgat
      181 ggtttcggtc gcgacgcaat cacggtcgag cagggtgcgg cagatggtgg cgcgaaccat
      241 ctcggaggtg agtaccgttc tgccgcagcg caacagtccg tctttgccga tttgcgatga
      301 agcgatctgt tgaatggcgg cgttcatggc ggcaacggcg gcgcgctgtg cctttttaac
      361 ggcggaaatt tcgtcagcgg tttttctttc gcgctgcgga cagagaggcg tttcgcttat
      421 ttcaagttta cgcccggcac gtttgagttt ttccgcgagg ccgaacggaa agccggaagc
      481 aaccgtcagt ttttttccgg ttacagtttt ttttatccag tcggcaggac cttttttcca
      541 ggaggatccg atcaattcgg gatgcacgac agcgatgctg cgtttgacgg ttcggcgcat
      601 gcggctgtat tccatttcgg aaacgatgag tgttccacgg gctccgttca ggataagcac
      661 cgcaggttca gggggttccg ctccgcacaa ataccggatg tcggcggcgt ttccggcgct
      721 gctgataagt atgtggacag gtgctgcttt tttcatatgc gtaaaggttt tgttagtggc
      781 gtgtttacaa aaaaaaggtg cgtttggtga gtttttcttt ttccgatctt tagacgttat
      841 tttgtgggct gtcctttttt ccaatctaca tcttgtgtcc ggtacggttt tttccggcgg
      901 gtcgggcttt gcccttgggc atctgtgctg agtttattag cttctccacg tcgcgaaagc
      961 gcagagggga ttcgcttgtt aataacctgt cgatatcccc ccttgaattg ttaatgattt
     1021 tgtaactgtg ccgatttgga gtcctatgcg caaagataaa gataagcttt gggaagatgc
     1081 ctgcagccac ctgcaggaaa cacttccgaa agatattttc gagaagtgga ttgctgttat
     1141 tcagtgtcgc agtatggaag acaccagggc tgtcctcgtc gtcggaaatg atttctacca
     1201 gtcgtggctc gaggagcatt acctcccgct tatccggaaa gcgctgggtg tcgtttcggg
     1261 ccgcgagatg cagattgaac tgacggtgga ctattcgctg atcacggaac agccggcaga
     1321 gaagagggat aaaactcctg ttgccgcgac ggcaaagaag ccggctgagc ataatctcaa
     1381 taaaaactac acgtttgact catttgttgt cgggccgtcc aaccagtttg cacatgcggc
     1441 gtccattgcg gcggcgcagg ccccgtcgcg cgcctacaat cctctcttta tccacggcga
     1501 tgtcggcctc ggcaaaaccc acctgatgca ggcgatcggt aattttattc attcgaagaa
     1561 ttcgcgcggc aaagtgtgct atgccacctg cgaatctttc accaatgaat atatcgaagc
     1621 gctgcagacc aatacactgg ccaagttccg taaaaaatac cggacggtgg acgcgctgct
     1681 gattgatgat attcattttc tcgccggtaa agaccggatg caggaggaat ttttccacac
     1741 cttcaacgcc atttttgaaa atcataaaca gatcgtgatg acatcggatc gcccggccgg
     1801 tgaattatcg gggctggctc cgcgtctggt ttcgcgtttt gaatggggcc tggtggttga
     1861 gctggaaagt ccggacattg aaacccgggt ggccattctc cgtaaaaaat gtgaacagct
     1921 gaatctgatg atcgatcaga gtctgctctt ctatattgcc gaacggattt catccaatat
     1981 ccgccgtttg gaaggtgcgc tgattcgcgt tgcttcctat gtttcgctga cggggcagag
     2041 tctggatcga tcgaagctgg aatatcttct gcgcgatacg ctggatcagg aaaagaaaaa
     2101 cgcgatcagt attgaaggga tccagaaggc tgttgcggag cattttgaca tccgggtcag
     2161 tgatctggtc gggccgaaac gtccgcagaa catcgcctgg cccagacagg ttgccatgta
     2221 tctggcccgc accatgacaa gccagtcgtt cccggtgatc ggtgatgcct tcagtcgtaa
     2281 tcatgccacg attgttcatg cctgccagct tgtggagcag cgtcagactg aggatatcaa
     2341 gctgagacag acactttcgt tgcttcgcca gcgcgtcagc cacagttcac agaaagccgc
     2401 tcagggaaat taacaacttc ccgatatcct gtggataagc tgggtataga aacaataact
     2461 tcgggttgct tgaaactcag cagagttcag cctgatttct taacagagat atccacaaag
     2521 ctaagtaggc tgattagacg ggtttaaaac aaaaaatagg ctgacggggt agtctattta
     2581 cagtactaat aataatacgg tttatataaa tttataatcc tattaattcc ggagggttgt
     2641 tgatgaaact cacagttgaa aaagatgctc tgcttgaagc gcttcaaaaa gttcaatcta
     2701 tagtcggaca gcgcagcacg cttcccattc tttcgaacgt tcttctgaaa gccgaaggcg
     2761 atacggtttc gttaaccacg accgatatgg aagtctgcgt taaaaccggc gctaaagccg
     2821 aaatcaatga gggcggcgga acaacactgc cggcccgccg gtttttcaat atctgccgcg
     2881 agctgcccgg cggacaggtt gagatcgaag tggatgccaa agacatagcc acaatccggt
     2941 gcggaccttc attttttaaa ctggtcggac tgccggaaga agaatttccg ccgctgcctg
     3001 agtttgaaga aagcgcggtt tacacgatcg atcagaacag tttcaaagaa atgcttcaga
     3061 aggtcgtgta tgcttcatcc accgatgaaa cccgctacat cctcaatgga gccttactca
     3121 gctttaaaga cgaaaagctg actgttgtag ccacggacgg acgccgtctg gcgctggtgg
     3181 agcaggaagt tgaattttcc gaagatgcac aggctaatct ggtggttccc actaaaaccc
     3241 tgaacgaact gattaaaacg cttggtgatg aaggtgtatt acggattcgc gtgtctgcca
     3301 cacaggtggc ttttgacttt gaccggattc tgattatttc aaaactgatc gaaggaacct
     3361 atccgaactt ccagcaggtt attccggcac aatgcgaaga acggattgct attgaccgcg
     3421 aaaccatact gaatgccgtc cgccgcgtat cactgctcac cgacgatcaa acggcgtcca
     3481 tccggctgaa cttcggaaaa aataaactcg aactggttac caacagtccg gacatcggcg
     3541 aagcgcgtga aaccattccc gtaaaatatg aagggaaaga aatttcgatc gcctttaatc
     3601 ccggcttttt aatggctccg ctccgccatc tggattctga cgaaattttc tttgaactgt
     3661 cggatgaatt gagcccggga gtgattaaga ccggcgtacc gttcctgtat gtgctgatgc
     3721 ccatccggat atcataaaag ggctggatgg ttgcccgact aggatttgaa cctagacaag
     3781 cagattcaga gtctgctgtg ctaccattac acaatcgggc agaagaacgg gaaataagcc
     3841 agagattctt gaagagtgca agtgggaata gataaaaaga gatgagagag ggcggacaaa
     3901 gccgggtgat agggctgctg ggtgtcggat ttgaccacgc agatggacaa atccggatca
     3961 cacaggcgga tgactatcaa gtgctcatgg gatccggaga aacccatgaa gagctgcaga
     4021 aaatctgcgg ccggattgat gaatgcatca aagcttccgg ccgggcgctg agcgactata
     4081 ctccagaaga attcatggag atggtgcagg agctttactg agaaggcgcc gccatgaaga
     4141 tactctttct caccaacaaa cttcctcacg ccgaagtggc gggcggacac cgtcttattt
     4201 atcagcgcat ccggtaccta tccgaacgcg ggcaccggat cgggctgttg acctttgttc
     4261 aaggtgaaac cgccgcccag atcgaatcgt taaagcccct tctgaccgaa cttcatacct
     4321 tacctcaccc ccggcggaac atttttatcc gcgccctgca tgactatctt tctctaagcc
     4381 gtccggcggt tttctggaaa agctattcac gcgacatgat gaaagaagtc gggcaggtgg
     4441 cggaacaggg gaagtacgac ctcgtgattg ccgagttctc cgaaatggga cagtacctcc
     4501 acaaaaatcc gtatctctcg gcgattcaca aagtgatttc ctgccaccgg tgcgtaacgg
     4561 cttcttatga aaagtacaaa tccctgggcg aagtgagatt aaagctgtac ctgaaaagca
     4621 ttccgcagct tcggggcctt caaaaatatg aattcaatat gtaccgcagc gcagaccgca
     4681 tccttgttct cacgccgcag gaccggttta ccatgcaata ttatgcgcag gatcttgcga
     4741 tttctgtcgc gccggccggc gtagacatcc ggtatcttca ggaacatccg ccggtaccca
     4801 aggaaccgat cgtcctgata accggcttta tgagcgaccc tgccaacgaa gacagcgtcg
     4861 agtggtttta tcatcacgtg tggccgcgtc ttagcgagcg gcatcccgaa gtaaaattct
     4921 atatcgtcgg cgccggtgtc cggccgagca ttaagcgcct ggcggaaaaa gactgccgga
     4981 tcatcgtcac cggagaagtg gaggacatgc gtccgtaccg caaccgggcg cgcgtttttg
     5041 tttcgccggt ccggctcggc tccggcatgc gcaccaaagt acttgaagcc atggccggcg
     5101 gcgtaccggt cgtctcgaca tcgctgggta tggccggcat cgacgcgcag accggcgtca
     5161 actgccttgt tgccgacaca cccgaactct tcacacaaag cgtcgaatgg ctgctgacgg
     5221 accgggcgct cgccgcacgg atggtccgca atgcccggga gcttgtcgca aagaaacact
     5281 cgcttgaaag cgggatgcgc cgttttgaga acatcatcac ctccattgcg gaaggatagc
     5341 catatctgca gggctgccgc cgatccggag catgaccgcc tcgttatcga cgagtgtaga
     5401 aacgaaatcc ttcaagctgc ccgtacccgc cggcgcggaa ccggactcct cagccgcgaa
     5461 taaatttcca aacctctgaa aatcggctcg ctattttcct gtatttggaa agaatggttt
     5521 acattttttc gaacatggga aagcagagaa tggcgttacc gtttacacag aaaatcctgc
     5581 aggactgggc cggctggaag tcgtttcgcg acggcaaact gctgttcgaa cgcggcgtgg
     5641 tggaaaaaat agaatacaac catccgttca tctccggcga gctggccgtc ggtatgcgag
     5701 ggatgcgcac caaatttgaa atcctgcctg acgggctggt tgaaaaccac tgcccgtgcc
     5761 gcgacaatca ggaacgcggc ctcatctgcg cccaccttgt cgcgctcggc cttgaagcca
     5821 tccggctgta caacgatccg ctgaggcagg aaaaagccct gaaagaaaaa cagcgcgccg
     5881 cacgcctcga acagctcgac gaaagcaaat acatcacccg ggttccggcc gggcatccca
     5941 aggcggtttc ggcggtgctg cgcctccagc tcgaacagaa ctggcgcgaa cagtcgctcg
     6001 gcggacggtt tccgctgcgc tgttttgcca gctacaacgg gcgggatatt cctctgaacg
     6061 ccgtgccggc ggatgttccg ctctccttca cgccgcggga tgaaaacctg ctgtttgttc
     6121 tcgaagatat ttgcgaaggc ccggcccgcg gacggctcgt cgccggcgaa gccgacttca
     6181 tcaacctgct tcaactgtgc atcaacaaac cggtatttga agaaggcgaa acccggccgc
     6241 tgacggttct gcccgaaacc gtcggcacca ttctccgcat ggagctcgac gaagcgaccg
     6301 gcgaactcaa cctctccatg catgtgaaat cctccggcgg cagcggcagt ccgcttttta
     6361 tcatcggtgc gcgtgaagga tggatctttg aacagaatac gttccggaaa ctggatgaac
     6421 tgctgcctgc gccgctgcgc agcatctacc acaacccgac acgcatcgcc cggcctgctg
     6481 tgccgcattt cctgcaccac gaacttccga cgctcgggaa cctgatcccg gttgatacga
     6541 aagtatcgcc ggatctcttc acgctgacac ctgccgagcc gcgcttccgt ctggtcgtca
     6601 gcggcagccg cgcctcgctg tccgctaaac tctacgccga atacggcgag atcgtcctcg
     6661 tcgccgcccg cgacgaagcc gccggcggat tcgccattcc gtcgccggac gacattctcg
     6721 aataccgcat gaggaatccc gaagcggaaa aacaggccct tgaaaaactg gcgacgaccg
     6781 gcttccgcgg gcaccgcggc gatctgctca cctccatcgt cggtccgcgc gaagtgctga
     6841 actttctcgg cggctccatt ccccgcctgc gccgatacgg ctggcgcgtg gagctggaag
     6901 ggcgcgtcgc cccgttcatg gacgaagcgg aattcaccac gcctgtgatc cggatcaacg
     6961 aagcttccgg cggcggcttt ttcgacgtcg gctacgccta tgaaaacagc ggcgggcaga
     7021 gcctgagcga aagcgaaatc cagcgcgcgc tcaacatggg cgaagcattc gtcgaaaaga
     7081 acgggcgcac catcctgctc gacatcgacg cgatcgaaac cgcgcgcgaa gtattcagcg
     7141 actgcgcttt cggcgcgggc ggcgcggcgg gcacgttccg catgaacgat atttacgccg
     7201 cctatgttca ggcctcgctt cattcgctcg acggcgtgga tgtcgaagca tcgcccgact
     7261 ggatgaaaat ggcgcagacg cagaaccgtg cgcagaaaat cgagccggaa ccgcttggcg
     7321 aaaaacttga agccactctg cgcgattatc agaaagacgg cgtctactgg ctgcgcttcc
     7381 ttgagcgcag cggcttcagc ggacttcttg ccgacgaaat gggtctcggt aaaacccttc
     7441 aggcgctcgt ctggcttcag ctggaacgcc acaatgaaaa agcgcgcggc aaaccggcgc
     7501 tcattgtctg cccgaccagt ctggtggaca actgggcgga agaggctgag aaattcacgc
     7561 cgcatctgcg cgtactcaaa ctgcacggcg cggaacgcca cgaccactgg gaaaaggtgg
     7621 gcgaaagcga tctgatcata acgtcctacg cgctgctgcg ccgcgacatc gaaaaatatc
     7681 tggaacacga attcgccgtc gcaattctcg acgaagccca gcacatcaaa aaccggtcga
     7741 cccagaacgc ggtcgccgcc aagaaaatcc gcgcgatgca caagctggtg ctcaccggta
     7801 cgccgattga aaacagcgtc gccgacctct ggtccatcat ggacttcctg atgcccggct
     7861 atctcagcaa tcacaaagcc ttccgcgaac actacgaact gccgatcggc cgcggcggcc
     7921 cggacggcga actggcgcag ctgaaactgc gccgcaaact gcatccgttt ctgctgcgcc
     7981 gcctgaaacg cgaagtcgcc aaagacctgc cggaaaaaat ccagcgcgtc gcgcacagct
     8041 cgctcagcaa agaccagtac atggtctaca aagaactgct cgaaagttcg aaacggaaaa
     8101 tcagcgatat ggttgcttcg cagggcttca accggtcgcg catgcagatt ttcaaaaccc
     8161 tgctgcgtct ccgccagacc tgctgccatc tcgacctgct caaactgccc ggactccaaa
     8221 gcgaatttcc gtccgccaaa atggaactct tcttcgaact ggtggacgaa gccatcgacg
     8281 cgcgccaccg gattctggtt ttcagccagt tcacttcgat gctcgccatc ctgcggcgcg
     8341 agctggaagc ccgcgaactc aaatactgct acctcgacgg cgccaccaaa gagcgccaga
     8401 gcattgtccg ccagttcaac accgaccgct ccattcctgt ttttctgatc agtctgaaag
     8461 cgggcggaag cggactcaac ctgaccggcg ccgacatggt catccactac gacccgtggt
     8521 ggaatccggc ggtggaagat caggcgaccg accgcgcgca ccgcatcggg cagaaaaata
     8581 cggtgtacag cgtcaagctc atcaccaaag acaccgtcga agaaaaagta cttcagatgc
     8641 agcagcggaa acagggcgtc atttccgcca ccctcgaaaa agagggcgaa ttcatgcaga
     8701 cgctcacctg gagtgatgtt caggagctcc tttctctcta acggatccgg cggcgtatga
     8761 cgaaaaaaca aatccatccg gatgatatgt cgcgctggca gctgatgttc cgggcgctga
     8821 gtcaccgtaa cttccgtctt ttcttcatgg ggcagagtat ttcgctgctg ggcacctgga
     8881 tgcagatgac ggcggtggcg tggctggtct ggcgtctgga gcacaacgcg tttctgctcg
     8941 gactggccgg ttttgcgtcc cggattccgg cgctcgtcat ggcgccgctg gccggagtcc
     9001 tggtggaccg ctggaaccga taccggctgg tcatcattgc gcaggtgctc gccatgatac
     9061 aggcgctgct gctggcggcg ctgctgtatt cgggccacgc ggccatctgg tctgtgatcc
     9121 tgctttcact gtttctcgga ttcatcaacg cgctggatat gccggcgcgg caggctttcc
     9181 tgatccagat gctcgaccgg cgggaagatc tcaccagcgc gatctcgctg aattcagcga
     9241 tggtcaacgg cgcgcgtctg atgggtccgg tggccgcggg actgattatc gccgccgccg
     9301 gcgaaggact ttgttttctg atcaacggcc tcagctatat tgcggtcatc gccggcctgc
     9361 tgatgatgcg ggtttcgccg caccattatg tccggccgga aaccaatatc gcccataacc
     9421 tgaaagaagg cttcaggtat gcgttcggat ttccgccgat ccgggcgctg ctgctgctga
     9481 tcgctctggt cagccttgcc ggagcgtctt acgcccagct gctgccggtc tttgcgcagc
     9541 agattctgca cggcggtccg cgtacacagg gtttgctggc ttcggcggcc ggcctcggcg
     9601 cgctgaccgg tgccgtcctg cttgccacgc ggcgcagcgt ccgcggcctc ggacggtgga
     9661 ttgcgtgcgc gccggtcgtc ttcggcctcg ggctgatcgg catgggcctt tcctcctcgc
     9721 tctggctgac gctggcggtc atgccggtcg tggggatcgg catgatggca cagatggcgg
     9781 cgaccaatac cgtgctgcaa accattgtgg atgacgacaa acgggggcgc gtgatgagtt
     9841 tttattccat ggccttcatg ggaaccgttc cgctcggcag tctgctggcc gggtcgctcg
     9901 tccatctcat cggcgcgcca tggaccgtca tatcaggcgg cctctgctgt attgccggag
     9961 cggcggtttt cgccacgaaa ctcaaaaaca tcaaccggct gatccacccg atttacgtcc
    10021 gcaaaggcat cattccggaa gtcgccgccg gattgcagtc cgcttccggc gtaacccagc
    10081 cgtaaggatt ttatacttcg gcgcaaccgc ggcggctgac tggcggaaac caaaaccgcg
    10141 gctctactgc ccgcagcatt tcttgaattt tttaccgctt ccgcacgggc aggggtcgtt
    10201 gcggccggtt tccgggccgg tatgacggat cggctgcggc gcggcttccg cgacgggcgg
    10261 cgcgccgtga ccgagctgct gctgtctggc ggcggtcatg gcagaaggct gttgcgcctg
    10321 ctgcgcccgg ctgttcatga tcattttatt cagcgactgc cagaagttct gcagcgcctc
    10381 catggaggag ccggtgcgga aaatgttctg cgcgatttcg tt
//
LOCUS       DAOPZO010000065        10391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_7453_length_10391_cov_7.714396, whole genome shotgun sequence.
ACCESSION   DAOPZO010000065 DAOPZO010000000
VERSION     DAOPZO010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 10391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10391
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7453_length_10391_cov_7.714396"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="PKI68_05545"
     CDS             complement(<1..1039)
                     /locus_tag="PKI68_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNX53188.1"
                     /translation="MATASKVLGIDLGTTNSCMAIMEGGEPTVIPNAEGGRTTPSVVA
                     FTKDGDRLVGQAAKRQAVTNPKNTIFSVKRFMGRNYSEVAHEIELVPYQVVKASNGDA
                     HIKIGDKTYAPPEISAMILQKMKTDAEAYLGEKITQAVITVPAYFNDRQRQATKDAGK
                     IAGLEVLRIVNEPTAASLAYGLDKKHDEKIAIYDLGGGTFDISVLDIGDGVFEVAATN
                     GDGHLGGDDFDQKVMDWLVQEFKKEQGVDLSKDPMAQQRLKEAAEKAKCELSSSNSTD
                     INLPFITADASGPKHLNVTLTRAKLEQLVDDLIARAEGPVKTCMQDAKLSNSQIDEVI
                     LVGGSTRMPRVQ"
     gene            complement(1235..2185)
                     /gene="rnhC"
                     /locus_tag="PKI68_05550"
     CDS             complement(1235..2185)
                     /gene="rnhC"
                     /locus_tag="PKI68_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HNX53189.1"
                     /translation="MKQNSFTIKLTGEQQEKLTALLRASHYTPVEVPYTTIAVDTGTC
                     RVNVYTSGKCLVQGKGAEEWITFTLEPEVLGEARLGYDAVHDPESVQPHMGVDESGKG
                     DLFGPLVIASVYTDAKSVEILKELGARDSKKISSDRVALQLASEIRKKLDGKYAVITI
                     GPEAYNRMYAKIGNVNRLLAWGHARAIENLLEKVPDCPRALSDKFGSTVLIERALMEK
                     GKKIKMDQRTKAESDPAVAAASILARAGFLLALRKMEKDFGIETVPKGCSAKVKQIAT
                     ELVANKGPGILLKTCKCHFKTTAEVLGANGYSLSDLPSAS"
     gene            2307..2774
                     /locus_tag="PKI68_05555"
     CDS             2307..2774
                     /locus_tag="PKI68_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53190.1"
                     /translation="MNLLRYIPVFLILAACNAQASSPTFGDLAVLVAKGYFGNYVKQD
                     SSLEECVAFLNRNGISFSLFDLMDPDKVVKNEDFARVVGQSTLLFSGEAEVVGGHIKK
                     PAEAETWVDYCLLNDIDLRPVWDGFVQRTANGPTPEVVKFLGLQAGGKKGAKK"
     gene            2771..3337
                     /locus_tag="PKI68_05560"
     CDS             2771..3337
                     /locus_tag="PKI68_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53191.1"
                     /translation="MKTVKFRLLVVLIAVSGFMISPVLADLGLQQLAAAQGAPAVGQA
                     VLTAAKAVYANNTDPAVIQQQLIAILNEAAATGNEQAVRYAIVAVMLAGGVDNIGLSK
                     SAINNSNVFLNYEALTAVTVAAAESLMTASGGSNGTSGGNNMGGGGAGGGTTGGDQTL
                     GGGSDIENPFNWSTLIGPGAISVSATPI"
     gene            3404..>3913
                     /locus_tag="PKI68_05565"
     CDS             3404..>3913
                     /locus_tag="PKI68_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53192.1"
                     /translation="MNRGKFLIPVLVGLMIGQIFADNERPFGLANTVRFGYSDNIYRN
                     PEGKNSPFITDIVDLSFKAALSDRTDLIVKSQLVLLDDNANNGNQLYPNLYAMLNHNI
                     SPRLLLRLSDVYRSGEKSGQGIGSSANARYNYFDNKVTGSADYVLTEKDRLGASLSHE
                     ILRHDKELSD"
     assembly_gap    3914..4013
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4148..6277
                     /locus_tag="PKI68_05570"
     CDS             4148..6277
                     /locus_tag="PKI68_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNX53193.1"
                     /translation="MSENLKKETTLHFLDYWRVIRSRKEIVLAVLFLVVITGTAYTFT
                     LPKMYAAQARIEVNQDNVEIDPFNQSMGGGGRAYDPYFLRTQFEIIQSKPILYEVINR
                     LNLQEIWGKKDEKISKEVAYKILRSSLEASQYRDTSLIALTATRQDPKEAARIANELA
                     SVYRDARLNLKQKEMTRAMDALSRELEKQQEKVDLAQQRVEKLRQELDITVLPGAYAG
                     QGENSVEKLRLQQLEGDRISSRVEMLVAKARYEQLESMNNEDILTASSYLAQDPFVET
                     MRTQIKDYDAALASLLQNYGPNYPEVKQTQAARDELMAKLQRALEGIKKGTQAQYIVA
                     KSKFEALEEELRGVKSSDIESQREKFVPFNKAQQELELQRSIMNALKSRIAQEGITLE
                     VPRTPVEIIDSAEESSRPVSPNLILNVLMSIFIGLGSGIGLAYFIEYLDTSIKTADDV
                     ERWIELPVLGLIPQNVRPLIEEGPDSEHAEGYRVLRTNMAFADAGGPRKGAFAVLSGG
                     AGEGKSTTAFNLAYVCAQQGEKVLLVDADLRRPVQHTILGVSNRFGLTNVLLRDVPVE
                     ETIKTTSVPNLHFLPSGRLPRTSLGILDPKRISELVYSLKQKYDIIIFDTPPLVGISD
                     SAVIAKEMDGVVLVVQYRKYPRDMIIRAKQMLDTLGIAQVGVVLNNINIMRDDYYYYY
                     HSYYSNYHYYRSQEANPVVSQGTQGAA"
     gene            6288..6866
                     /locus_tag="PKI68_05575"
     CDS             6288..6866
                     /locus_tag="PKI68_05575"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNX53194.1"
                     /translation="MKIKDIFLVLMAAFVLTGCTSHPVAVPDDTSVVGLYQLRALDPL
                     VISLRGIPQEKQIESIIDERGTITLPYVDAPVQAAGMTTSELERKIQSIYTDGKIYRN
                     ITVNIATTAKIYYMEGEVRKPQEYLLNRRITLLQAIAAASGYTEYANPKNVTITRNGK
                     IMKFNTVDLEKHPERDVPVEAGDRIKVNRSVF"
     gene            complement(6913..7314)
                     /locus_tag="PKI68_05580"
     CDS             complement(6913..7314)
                     /locus_tag="PKI68_05580"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNX53195.1"
                     /translation="MKKNLDQQLKWELARERFRLANPFPPPEQRPEKCISDILAGILR
                     KDESETASLPEKITDRWPVVAGEQLAKHTRPAHLKNGILYINADHPGWLTELRRLPKA
                     HMLKKIASIPDIPEIKDIRFQLDPAIRSGRK"
     gene            7395..8666
                     /locus_tag="PKI68_05585"
     CDS             7395..8666
                     /locus_tag="PKI68_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNX53196.1"
                     /translation="MNSIHQYMFSLLLMLALLALSAFFSCSETALFSLPPDAARRLRE
                     HRRIDDLLTILHKEPSGLLAAILFGNLVVNVLFFCTGAEAAGRLAEERGGWFEALGGA
                     LVLLTVILFGEIIPKAIGITHPSGVLHITATPLRAWFYFTRPFRLFIRWLLDRIGLRD
                     GEASRRAYITPGELKELLDAVQHEPGFGSHEKEILEDIVNLPDVRVREVMVPRVQVLR
                     RPLDADRDEMLSEALRHKYSRVLIYRDKDDEPLGYINVRDIFMDGGRTVSLEPFIRPL
                     VFVPETKRADALLREFMSRDWQMAAVVDEYGGLAGVVTIEDLLAEVVGDFESGTADEI
                     QKLDEVTYRLHGQLSIRAWRELFAGFLPGQEMDALAFNTLGGFIISLLGRMPHQGDTV
                     SVRNLQLTVETMQHRRIGTVLLSLNEPEAAA"
     gene            8663..9670
                     /locus_tag="PKI68_05590"
     CDS             8663..9670
                     /locus_tag="PKI68_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HNX53197.1"
                     /translation="MNGWLSIGLIAAALAASMLFSGVETGGYLLNRIRLRSRVRQGDL
                     AARRLQNSLSDAHRFIFTVLISNNIANYMISREVTRLYSESGLTTGGRLIFGFIPWNA
                     ETAATLTLLLPLFLFGELIPKNIFRHYAETLMYRCAGLLRLVQWVLSPVTAVLKALFN
                     LLTGGRGRREDLNGFSLSLQGLREYFSEDTRRASLSAHQHGMIDNLVAMHRIPSQQVM
                     VPAAGMVQVSEKASVRQVFDLMRTRGVDQVAVYRGPARSVTGFIDLFDLMDPMLNPDD
                     PVKPLLHKSVRVTADMPLTRAFRLLRKRGEQMAVVVDRSVRVVGLLHIRDIARYIVRD
                     V"
     gene            9777..>10391
                     /locus_tag="PKI68_05595"
     CDS             9777..>10391
                     /locus_tag="PKI68_05595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX53198.1"
                     /translation="MANEDKKHVYDESKIKTLSSLEHIRARTGMYIGRTGNGSHYDDG
                     IYVLLKEVVDNAIDEFIMGHGKKVEITIEEDTVNVRDYGRGIPLGKVVECVSRINTGA
                     KYNDDVFQFSVGLNGVGTKAVNALSSYFIVRSHRDGEFVEARFARGILEAEEKGKSKT
                     EENGTFISFSPDPEIFKVYKFREDHIERRLRFYSYLNAGLTLLFN"
BASE COUNT         2306 a         2479 c         2902 g         2604 t
ORIGIN      
        1 tctgaacgcg cggcatacgg gtcgaaccgc cgaccagaat cacttcgtca atctggctgt
       61 tgctcagctt ggcgtcctgc atacaggttt tgaccgggcc ttccgcgcgg gcaatcagat
      121 cgtccaccag ctgttccagt ttggcgcggg tgagggtgac gttcagatgt ttcgggccgc
      181 tggcgtcggc cgtgataaac ggcagattga tatcggtcga gttcgagctg gaaagttcgc
      241 acttggcttt ttcagcggct tctttcagac gctgctgagc catcggatct ttcgaaaggt
      301 cgacgccttg ttcttttttg aactcctgaa ccagccagtc cattaccttc tggtcgaagt
      361 catcgccgcc gaggtgtccg tcgccgttgg tagcggccac ttcgaacacg ccgtcgccga
      421 tatcgagcac ggaaatatcg aatgttccgc cgccgaggtc gtaaatcgcg atcttttcgt
      481 catgcttctt gtcgagaccg taagccagcg aagcggcggt cggttcgttc acaatgcgca
      541 gcacttcgag accggcgatc ttgccggcgt ctttggtggc ctggcgctgg cggtcgttga
      601 agtaggccgg aaccgtaatc accgcctgag tgattttttc gccgaggtag gcttccgcat
      661 cggtcttcat cttctgcaga atcatcgcgg aaatttccgg cggcgcgtag gtcttgtcgc
      721 cgatcttgat gtgggcgtcg ccgttcgagg ccttgacgac ctgatagggg acgagttcga
      781 tttcgtgggc cacttcgctg tagttgcgcc ccatgaaacg cttcaccgag aaaatcgtgt
      841 ttttcgggtt ggtgactgcc tgacgtttgg ccgcctggcc gaccagccgg tcgccatctt
      901 tggtgaaagc cacgactgac ggagtggtgc gcccgccttc agcattcggg ataacggtcg
      961 gttcgccgcc ctccataatc gccatacagg agttggtggt cccgaggtcg attcctaaaa
     1021 cttttgatgc agttgccata gttatgttcc tttcagttaa aaccaaaaat ctgccatcag
     1081 aaaagcaggc agcgtgccga ggagatgttg ttagttatta gttattagtt attagctggt
     1141 tgcgaaaata tcagttcaga cgcgctggaa aaatatcggt cggatatgtc ccattcagga
     1201 gtcgtgtaga gccagaatgg cgcaactccg caactcagct ggcagacggc aggtcagaga
     1261 ggctgtatcc atttgcgcca agaacctccg ccgttgtctt gaagtgacat ttgcaggttt
     1321 tgagcaggat gccgggacct ttattggcga ccagttcagt ggcgatctgt ttaactttgg
     1381 ccgagcagcc ttttggcacg gtctcgatgc cgaagtcctt ttccattttg cgtagcgcca
     1441 gcagaaaccc ggcgcgggcc agaatggatg cggcggcgac ggccggatcg gattccgctt
     1501 tcgtacgctg gtccatttta atttttttac ctttttccat cagcgcgcgc tcaatcagaa
     1561 cggtcgatcc gaatttgtcg gaaagcgcgc gggggcagtc cggcactttt tcgagcagat
     1621 tctcgatggc gcgggcgtgg ccccacgcca gcaaccggtt gacgttgccg attttggcgt
     1681 acatgcggtt gtaggcttcg ggcccgatgg tgatgacggc atatttcccg tccagctttt
     1741 tccggatttc ggacgccagt tgcagcgcaa cccggtcgct ggaaatcttt ttactgtcgc
     1801 gcgcgccgag ttctttgagg atttcgacgc ttttggcgtc agtgtagacg gaggcgataa
     1861 cgagcgggcc gaaaaggtcg cctttgccgc tttcatcgac ccccatgtgc ggctggacgc
     1921 tttcgggatc atggacggcg tcgtagccga ggcgtgcttc gccgagcact tcgggttcaa
     1981 gagtgaaagt aatccactct tcggcgcctt tgccttgaac aagacatttg ccggaggtgt
     2041 aaacattaac gcggcaggtt ccggtgtcga cggcgatggt cgtgtagggg acctcaacag
     2101 gagtgtaatg agacgcacgg agcagggctg tcagtttttc ctgctgttcg ccggtcagct
     2161 ttatggtgaa tgagttttgt ttcattgccg gaagttctta gcagagtttg gttttgatgg
     2221 aaaagcaaaa ggtgcaaagg gttgtgtgac cgggttttgg gggttgctag aaaaggagcg
     2281 cctgtgatag acattgtcca taaagcatga accttctgag atatattccg gtttttttga
     2341 ttttggcggc ttgtaatgcg caagcttctt ctcccacctt cggcgatctg gctgttcttg
     2401 tggcaaaagg ttattttgga aactatgtga aacaggattc ctcgctggag gagtgtgtgg
     2461 cctttttgaa ccgtaacggc atttcatttt cgttgtttga tctgatggat cccgacaagg
     2521 ttgtgaaaaa tgaagatttt gctcgggttg tcggtcaatc cacgctgctc ttttcaggcg
     2581 aagctgaggt tgtaggcggg cacattaaaa aaccggcgga ggcggaaacg tgggttgact
     2641 actgcctcct gaatgatata gatttgcgac ccgtttggga cgggtttgtt cagcggacgg
     2701 caaatggccc gacgcccgag gttgtgaagt ttttagggct gcaggctggc gggaaaaaag
     2761 gagctaaaaa atgaagacag taaaatttcg tttgcttgtc gtgcttatcg cggtttccgg
     2821 atttatgatt tcacccgttt tggccgatct cggtttgcag cagcttgcgg ctgcgcaagg
     2881 ggctccggca gttgggcagg cggtgttgac ggcggcaaag gccgtctatg ccaacaatac
     2941 agatcctgct gttattcagc agcaactgat cgctatttta aatgaagcgg ccgcgactgg
     3001 aaacgaacag gccgttcgtt atgcaattgt cgctgttatg ctggctggcg gggtggacaa
     3061 cattggattg agcaaatccg ccatcaataa cagtaatgtc tttttgaatt atgaagctct
     3121 cactgctgta acagttgctg ctgctgaatc tttgatgacc gcatccggcg gctctaatgg
     3181 aacgtctggt ggtaataata tgggtggcgg cggtgcaggt ggcggcacga ctggcggtga
     3241 tcaaactctc ggcggcggtt ccgatataga aaatccgttt aactggagta ctttgattgg
     3301 acccggggcg atcagtgttt cggcgacacc gatttaacgg agcagaatta acaggcaaaa
     3361 cgaattattt ttgatgagat tcagtttttc ctgaaggggg cgtatgaata gaggaaaatt
     3421 tttaataccg gttctggtcg gattgatgat cgggcagatt tttgcggata atgaacgccc
     3481 ttttggtttg gcgaatacgg tgcgctttgg atatagcgac aatatttacc gaaacccgga
     3541 aggaaaaaat tctccgttta ttacagatat tgtcgactta tcgttcaagg cggcgctttc
     3601 agatcgtacc gatttaatag tgaaatccca gctggtgctt ctggatgata atgccaataa
     3661 cggtaatcag ctttatccga acttatatgc gatgcttaac cacaacattt cgcctcggtt
     3721 gcttttgcgt ctttcggatg tctatcgcag cggagagaaa tcaggacagg gtatcgggtc
     3781 ttcggcgaat gcccggtata attatttcga caacaaagtt accgggtcgg cggattatgt
     3841 tttgactgaa aaagaccgtc tgggagcttc tttaagccat gaaatattgc ggcatgataa
     3901 agaactcagc gacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngattttg
     4021 actggaaaga aaaccgggtt gatgtcggct ggcgtgttga gttgaactag aaaaaaatac
     4081 aggccctggc tttcaaaatt atggtgaggt ttgcgcctca ccatcatcgt ttaataggag
     4141 cagtataatg agcgaaaatc tgaagaagga aacgacattg cactttttgg attactggcg
     4201 ggttatccgt tcccgcaaag agatcgttct ggctgttctt ttcctcgtgg tgattacggg
     4261 aaccgcctat acctttacgt tgccaaaaat gtacgccgcc caggcccgaa ttgaggttaa
     4321 tcaggataat gtcgaaattg acccgtttaa tcagtcgatg ggcggcggcg gtcgcgccta
     4381 cgacccgtac tttttacgca cacagtttga aattattcag tcgaaaccga tcctttacga
     4441 ggtaatcaac cggctcaatc tgcaggaaat ttggggaaaa aaagacgaaa aaatctccaa
     4501 agaagttgcg tacaagattc tccgctcaag tcttgaggca agtcagtatc gtgatacaag
     4561 cctgatcgcg ctgacggcca cacggcagga tccaaaagaa gctgcccgga ttgctaatga
     4621 actggcgagt gtctatcgcg atgcccgcct taaccttaag caaaaagaga tgacacgcgc
     4681 tatggacgcg ttaagtcgcg agcttgaaaa gcagcaggaa aaagtggatc tggctcaaca
     4741 acgggtggaa aagctgcgcc aggaactcga catcacggtt cttcccgggg catacgccgg
     4801 tcaaggcgaa aacagcgttg aaaaactccg gctgcagcag ctcgaaggag accggatttc
     4861 ctcccgcgtt gaaatgctgg tcgcaaaggc ccgctatgaa cagctggagt ctatgaataa
     4921 cgaagacatc ctgacggctt cctcttatct cgcacaggat ccttttgttg aaacgatgcg
     4981 cacgcagatt aaagattatg atgcggcgct ggcctcgctt ctccaaaatt acgggccgaa
     5041 ttatccggaa gtgaaacaga cgcaggctgc ccgcgatgaa ctaatggcga aactgcagcg
     5101 cgcgctggag ggaattaaaa aagggactca ggctcagtac attgttgcca aatcaaaatt
     5161 tgaagcgctg gaagaagagc ttcgcggtgt taaatcgagt gatattgaat ctcagcgcga
     5221 aaaatttgtg ccttttaata aagcccagca ggagctcgaa ctgcagcgtt ctatcatgaa
     5281 tgcgctgaaa tcccgcattg ctcaggaggg gatcacgctg gaagtacctc gcactccggt
     5341 cgaaatcatc gactcggccg aagaatcaag ccgcccggtg agcccgaatc ttattctgaa
     5401 tgtactgatg agtattttta tcggccttgg atccggcatc ggactggctt actttattga
     5461 atatctcgat acatctatta agaccgccga cgacgttgaa cgctggatcg agctgccggt
     5521 gctcgggttg attcctcaga atgtgcgccc tctgatcgaa gaaggtccgg atagcgagca
     5581 tgccgaaggc taccgtgtac tgaggacaaa tatggcgttt gcagatgccg gcgggccgcg
     5641 caagggtgct tttgccgttc taagcggtgg tgcaggcgaa gggaaatcta caactgcttt
     5701 caacctggct tatgtctgtg ctcagcaggg tgaaaaagtg ttgctggttg acgctgacct
     5761 gcgccgtccc gtgcaacaca ccatcctggg tgtatccaac cgctttgggt taaccaatgt
     5821 gcttttgcgt gacgtgccgg tcgaagaaac gattaaaaca accagcgttc caaatctgca
     5881 cttccttccg agcggacgtc tgccgcgcac ttcgctggga atacttgatc cgaagcgcat
     5941 cagtgagttg gtttatagcc tgaagcagaa gtatgacatt attatctttg atacaccgcc
     6001 gctggtgggt atcagcgact cggctgttat tgctaaggaa atggacggtg tagtactggt
     6061 cgtgcaatac cgtaaatatc cgcgcgatat gattatccgc gctaagcaga tgctggatac
     6121 gcttggcatt gcacaggtcg gtgttgtact taataacatc aatattatgc gtgatgatta
     6181 ctactactac taccactcat actattcgaa ttaccattat taccgttcgc aggaagcgaa
     6241 tccggttgtt tcccagggaa cgcagggcgc cgcctaaagg agcaaggatg aagataaagg
     6301 atatattttt agttttaatg gcggcgttcg ttttgactgg ctgcaccagc catcctgtgg
     6361 cggtgccgga tgacacatcc gtcgttggac tgtatcagtt gcgcgcgctg gatccgctag
     6421 tgataagtct gcggggaata cctcaggaaa agcagatcga atcaattatt gatgagaggg
     6481 gaacgatcac acttccgtat gttgatgctc cggtgcaggc ggccggaatg acaacgtccg
     6541 aactcgaacg taaaatccag agtatctata cggacggaaa aatttaccgc aatatcaccg
     6601 tcaatatcgc tacaacggcg aaaatctact acatggaagg cgaagtgcgt aaacctcagg
     6661 aatatctgct gaaccgccgc atcaccttat tgcaggcgat tgccgctgcc agcgggtaca
     6721 ctgaatatgc caacccgaaa aatgtgacaa ttacccggaa tggtaaaatt atgaagttta
     6781 acacggtcga ccttgagaag catccggaga gggacgttcc tgttgaagcc ggagaccgga
     6841 taaaggttaa tcggtctgta ttttaattaa tccagccagc gatttgctgc cggtccggag
     6901 aggccggctt tttcatttcc ggccggagcg tatggcggga tccagctgaa aacggatatc
     6961 tttgatttca ggaatgtccg gaatcgatgc gatttttttg agcatatgcg ccttcggtaa
     7021 acggcgcagt tcagtcagcc agccgggatg atcggcgtta atatacagaa ttccgttttt
     7081 caggtgcgca ggacgggtat gtttcgcaag ttgttctccg gcaaccaccg gccatcgatc
     7141 cgtaattttt tccggcaacg atgcagtctc ggattcatct tttcgcagaa ttccggccaa
     7201 aatgtcgcta atgcattttt ccgggcgctg ttcggggggc ggaaagggat tcgccaggcg
     7261 gaatctttca cgggccagtt cccattttaa ctgctgatcc aggttttttt tcatatcttt
     7321 cttcggcaga tgacagtttg caaaacggtt ttaaaatcag cagactctaa cacacacgaa
     7381 ggatgtggcg caccttgaat tcaattcatc aatatatgtt ttccctgttg ctgatgctgg
     7441 cgcttcttgc gttgtcggcc ttcttttcct gctcggaaac agcgctcttt tcattgccgc
     7501 cggacgctgc gcgccgcctg cgggaacacc ggaggattga tgatctgctg acgatcctgc
     7561 ataaagaacc ctccgggctg ctggcggcta ttctgttcgg gaatctggtt gttaatgttt
     7621 tgttcttttg caccggtgcc gaagcggccg ggcgactggc ggaagaacgc ggcggctggt
     7681 ttgaagcgct tggcggcgcg ctggtgcttc tgaccgtcat tctgttcggc gaaattattc
     7741 ccaaggcgat tggaattacg catccgtccg gtgttctgca tatcaccgcc acgccgttgc
     7801 gggcctggtt ttattttacc cgcccgttcc gtctttttat ccgctggctt ctcgaccgga
     7861 tcgggttgcg ggatggtgaa gcctcaagaa gagcctacat taccccggga gagctcaagg
     7921 agctgctgga tgcagttcaa cacgaaccgg ggttcgggtc gcatgaaaaa gagattcttg
     7981 aagatattgt caatctgccc gatgtgcgcg ttcgggaagt tatggtgccg cgcgttcagg
     8041 tgttgcgccg gccgcttgat gcggaccgag acgaaatgct gagcgaggcc ctccggcata
     8101 aatacagccg ggtgctgatt tatcgcgaca aagacgatga accacttggt tacatcaatg
     8161 tccgggatat ttttatggat ggcggacgga cggtttcgct tgagccgttt atccgtccgc
     8221 tggtttttgt tcccgaaacg aaacgggcgg atgcgctgct gcgcgaattt atgagccgtg
     8281 actggcagat ggccgccgtt gttgatgagt atggcggact ggccggcgtg gttaccattg
     8341 aagatctgct ggcggaagtc gtcggcgatt tcgagtcagg gacggctgat gaaatccaaa
     8401 agctggacga ggtgacgtac cggcttcacg ggcagctttc cattcgcgcc tggcgggagc
     8461 tcttcgccgg atttctgccc gggcaggaaa tggatgcgct ggcgtttaat acgctcggcg
     8521 gctttatcat atcgctgctg ggccgcatgc cgcatcaagg cgacacggtg tcggtgcgca
     8581 atctgcaatt gaccgtggag accatgcagc accgccggat cggaacggta ctgttgagtc
     8641 tgaacgaacc ggaggcggcg gcatgaacgg ctggctgtcc atcgggttaa tcgcggcggc
     8701 tttagctgct tcgatgctct tttccggagt ggaaaccggc ggctatctgc tcaaccgcat
     8761 ccggttgcgt tcccgtgtac ggcagggcga tcttgcggcc cgccggctgc agaatagtct
     8821 gagcgacgcg caccggttta ttttcaccgt tttgatcagc aacaatattg ccaattacat
     8881 gatttcccgt gaagtgaccc ggctctattc agagtcgggg ctgacgaccg gcgggaggct
     8941 gatcttcgga tttattccgt ggaatgcgga gaccgctgca acacttacac tcctgctgcc
     9001 gctgttcctt ttcggagagc tgattcccaa aaatattttc cggcattacg cggaaacgct
     9061 gatgtaccgc tgcgccggac tgctccgttt agtgcagtgg gtattgtctc cggttactgc
     9121 tgtcctgaaa gcacttttca atctgcttac cggcgggcgg ggccgccgcg aagacctgaa
     9181 cgggttttca ctttcgcttc aggggcttcg cgaatatttt tccgaagata cgcgcagggc
     9241 ttcgctgagc gctcaccagc acggcatgat cgataatctc gttgcgatgc accggattcc
     9301 ctcgcaacag gtcatggtgc cggcggcagg tatggtgcag gtgtcagaaa aagcttctgt
     9361 ccggcaggta tttgatctga tgcgtactcg cggtgtagat caggtggcgg tttatcgcgg
     9421 gccggcccgc agcgtaaccg ggtttatcga tctgttcgac ctgatggatc cgatgctgaa
     9481 tcctgatgat ccggtaaagc ctcttctgca taaaagtgtt cgcgtaacgg ctgatatgcc
     9541 gctcacccgg gctttccggc tgcttcgcaa acggggagaa caaatggcgg tggttgtgga
     9601 ccgttctgtc cgggttgtcg gattgctgca tatacgcgac attgcccgtt acatcgttcg
     9661 ggatgtttga agaatcgctg aaaacccgat ggaacagggg ttgcgggatg ttccaagccg
     9721 tgctatatct agcggcttca agggtaaaaa gcccaatata tggtggtttt cagcaaatgg
     9781 ccaacgaaga caaaaaacac gtttacgacg aaagtaaaat caaaaccctc tcgtctctgg
     9841 agcatatccg ggcccgtacc gggatgtaca tcggacgaac cggcaacggc agtcattacg
     9901 acgacgggat ttatgttctg ctcaaagagg tcgttgataa tgccattgac gagttcatca
     9961 tgggccacgg caaaaaggtc gaaatcacca tcgaagaaga tacggtcaat gtgcgcgact
    10021 acggacgcgg cattccgctc ggcaaagtgg tcgaatgcgt ttcgcgtatt aacaccggcg
    10081 cgaaatacaa cgacgatgtt tttcagttca gcgtcggtct gaacggcgtc ggcaccaaag
    10141 ccgtcaacgc gctttccagt tattttatcg tccgttcaca ccgtgacggt gagtttgttg
    10201 aagcccggtt tgcccgcggc attcttgagg cggaagagaa gggtaaatcg aagaccgagg
    10261 aaaacggcac cttcatcagc ttttcacccg atccggaaat tttcaaagtt tataaattcc
    10321 gcgaggatca cattgaacgc cgcctgcggt tttattccta tctcaacgcc ggcctcacgc
    10381 ttctgttcaa c
//
LOCUS       DAOPZO010000066        10347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_7494_length_10347_cov_7.514283, whole genome shotgun sequence.
ACCESSION   DAOPZO010000066 DAOPZO010000000
VERSION     DAOPZO010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 10347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10347
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7494_length_10347_cov_7.514283"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2279
                     /locus_tag="PKI68_05600"
     CDS             <1..2279
                     /locus_tag="PKI68_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HNX53199.1"
                     /translation="RVASGDHVMKKMTRFIFLLLGASAGSLSAAVAPHVGFVYPAGGT
                     PGTTFTVTIGGQYLRDFKDIHLSNVPVKAQLTDYLRIYDSKEGGGLRRKKETLEAKMA
                     EATDARSKLQLQKQIEMLEPEIAMVAEVRRQDRMNPSMAAKKQFNPQISERITLEITL
                     PADAKPGEYELRVITTNGLSNPLLFEIGRLPEIAEVEPNDLTKNAAKLPVLPVLVNGQ
                     ILPGDVDSFRFHALKDQTLVIKASARSLVPYLADAVPGWFQSVLTLYDANGKEVAYDD
                     DYLFNPDPVLIYHVPETGDYTFSIRDSIYRGREDFVYRISIGELPFIERIFPLGGPAG
                     SELDVNLYGVNLPYKKIRLKTNADAPETQYVHVEKGGLISNSQPFGVSPFPDRSESEP
                     NNQFSEAVAVTNDTAINGTIGKPGDHDWFCFKGRKGEQKTIEVFARRLGSPMDSCLTL
                     FDTNQQVLAVNDDAEDKSFGLLTHQADSRIDFTLPETGIYYVRLDDQQNKGGDAYAYR
                     LVIGKEKPDFQLRVVPSSLRIPRNGTAIATVYAIRYGGFTDAISCSVRDAPTGIELQR
                     AVIPAGTNVAKIVITARPRAAEQMMTLDIEGVADCGSRTVRRHAVPAEDMMQAFIYRH
                     LVTAQQMLVQVTEPEPVSVVLTIPKEGFFRAHPGKQLVISASAQWAASAQKSIKLTLA
                     EPPEWLTLRTATWQAGNIILDISPNAEPGDTATVLLTGTVKVNKSPKDADFNPVMKFL
                     NNRTADFTIDAIPIQVVE"
     gene            2291..3376
                     /locus_tag="PKI68_05605"
     CDS             2291..3376
                     /locus_tag="PKI68_05605"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNX53200.1"
                     /translation="MTAQISKTNYDADVATLTLPSHAEHCITVQSEQNMNRVLSRAAE
                     ERDATILSQFVFAGRQHYPEFLAETGDTGWPVVWLQGDACKDGSMYSMQAVAISGIKP
                     QPVRLAGRDVGFTYEDKHARYCRLRGILPADLKASRAAQTHSAFQIMEAALKENGFRF
                     TDTVRTWIYLDQLLEWYGEFNRVRTDFFNEKDIFSHMVPASTGIGAANPFGAAIMMDV
                     LAVQPKTKQIEINEVISPLQNPALDYKSSFSRAVEMKFPTHRSLLISGTASIDPSGKT
                     AYLDDPEKQIRLTMDVVKAILSSRDMGWSDLFRGIAYFKDMDYLPIYRRIAAELGVPR
                     FPLAVSHADVCRDDLLFEIEVDAVKVR"
     gene            3438..4190
                     /gene="radC"
                     /locus_tag="PKI68_05610"
     CDS             3438..4190
                     /gene="radC"
                     /locus_tag="PKI68_05610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNX53201.1"
                     /translation="MPEKRVSEDGGAYQVRANDLPKALQPREKFDRLGAENLSDSDLL
                     ALLLRTGTAGRNVVELAEALLMQYGSLSALSRTSVAELQKIHGIGKEKAKILKAALEI
                     GRRLAQENVGENPRIASPEEAAAVLRERARSLDREVFWVLLLDTKNRLMLPPCEVSKG
                     TLNSSLVHPREIFKPAVQNSAASIILAHNHPSGDPAPSAQDIRITRKLVEAGKTMEIK
                     VLDHLIIGRKTRDGANDFLSLRETGLAEFEER"
     regulatory      4229..4369
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4441..5646
                     /locus_tag="PKI68_05615"
     CDS             4441..5646
                     /locus_tag="PKI68_05615"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF006803.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNX53202.1"
                     /translation="MSEKLFDSMEEILDAVRRGEMVLITDDENRENEGDVICAAEFCT
                     PEQINFMITHARGLVCVPVTAERAAHLGLKRMNISHEGDKFKTAFTISVDAADSTTGI
                     SAAERAHTVRMLVDKNTRPEDMVCPGHIFPLIAMNGGVLERAGHTEATIDLTRMAGLQ
                     PAGVICEVANDDGSMARLPDLIKFAKKHGLKMTSVAEIAKYRYITETLIRMERQVKMP
                     TDWGDFDLRIYSSVVDDKEHLALFTGHPTPEKPHPLVRVHSECLTGDVFGSQRCDCGA
                     QLQLSMQMISDYGYGALVYMRQEGRGIGLTKKIHAYELQDQGLDTVEANVRLGFAPDL
                     RDYGIGAQILKDLHMTGIRLITNNPAKIEGLDKYGIEVVERVPIVLPSTPHNARYLNT
                     KREKMKHLL"
     gene            5677..6156
                     /gene="ribH"
                     /locus_tag="PKI68_05620"
     CDS             5677..6156
                     /gene="ribH"
                     /locus_tag="PKI68_05620"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNX53203.1"
                     /translation="MGKGISKTELNAGGLRFAIVVSRFNEELTSQLADDAFRCLKENG
                     AKVPDIGIFRVPGAYEIPVIINELAASGKYDALIALGVVVEGETPHAQMIGESTGIAL
                     LDIACKHGVPVINEIVGTRNWEQAVARCTSSRTSRGWYAAEAAIETANVLKTIKGSK"
     gene            6153..6602
                     /gene="nusB"
                     /locus_tag="PKI68_05625"
     CDS             6153..6602
                     /gene="nusB"
                     /locus_tag="PKI68_05625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNX53204.1"
                     /translation="MSKVMGRRQAREWAVQFLFQTEFNPGDLEPALDLFWNDEEKKPS
                     DRDRTYVNEVVRGVIEKQRELDRTISKYTDNWDVDRLGVLDRIVLRVATFEMLFRGDI
                     PPVVSINEAVEIAKAYSGKDSGRFVNGVLDRIQKELDRPSRSTYTTQ"
     gene            6599..7603
                     /gene="ribD"
                     /locus_tag="PKI68_05630"
     CDS             6599..7603
                     /gene="ribD"
                     /locus_tag="PKI68_05630"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNX53205.1"
                     /translation="MSSHEPFMQRALALAKRGEGLTRPNPPVGAVLVRNGKIISEGFH
                     KKAGGPHAEVNCLKSASGNLESAILYVTLEPCSTYGRTPPCTDLILSRGIRCVVIGAK
                     DPNPAHAGRGIRILRRAGVEVITGICRTEAETLIAPFAKRILTGMPYVTLKLASTLDG
                     KIADASGKSKWITGPQARARVQELRRSADAIMVGAGTVRADNPSLLPRPAKGRAPWRV
                     IVGTDIPEKSKVLTDEAAGCTLVMDGSLKKILRSLGSRGVMHVLCEGGGELAGALVRA
                     GLVDEFALFVAPSLLGGNGVPMFGKKGWPLIKMPLLNLQALEKCGKDVFIRALPVRGK
                     "
     gene            7603..8235
                     /locus_tag="PKI68_05635"
     CDS             7603..8235
                     /locus_tag="PKI68_05635"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNX53206.1"
                     /translation="MFTGIIQRLGTVVDVKMHGAAGRITLKPNRPFDFPVHLGDSIAV
                     NGTCLTVAAIENGNFCFDVLTETFNKTNLGEKGPGAIVNLERALALGDTLGGHIVTGH
                     VDGTGRVRRIDQIDRDWKFEIECAQDLLMLMVYKGSIAMNGISLTVAELKDDAFVVHI
                     IPHTLEETDMSQFKPGTKVNLEADILGKHVQRILEFGGGQNYRVSFENKQ"
     gene            8245..8616
                     /locus_tag="PKI68_05640"
     CDS             8245..8616
                     /locus_tag="PKI68_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX53207.1"
                     /translation="MDRKKILLVDDEEAIRKMVRAVLGSELYEFAEAANGIAAQAILE
                     KQKFALVISDVIMPDCDGIELVMAIRRKLPEIKIIVMSGGGRVRAGHYLDLARKLGAT
                     RVFEKPFDTAELRQTVHDLLS"
     gene            8627..9202
                     /gene="pyrE"
                     /locus_tag="PKI68_05645"
     CDS             8627..9202
                     /gene="pyrE"
                     /locus_tag="PKI68_05645"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNX53208.1"
                     /translation="MKETDLLKLFEDTQALLNGHFELRSGLHSNRFFQCALLLQYPRI
                     AEQVCTALVEKMKTVLGELDVDTVISPALGGIPVGHEVARSLGKRFIFAEKNADKMLT
                     MRRFKIAQGERFLVAEDVITRGGRVQETIDIVKQHGGIVHSVGVLVNRSGGKAQFDYP
                     TVSLLDIEPVTWEPAECPLCRQGLPLVHPGS"
     gene            9284..9988
                     /locus_tag="PKI68_05650"
     CDS             9284..9988
                     /locus_tag="PKI68_05650"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNX53209.1"
                     /translation="MAHPLEAFLVLQEKDRKIAKLQREIRDIPARKADIEKQLEGSKK
                     RLAEARELLKKATADLKQLEVEAGAHRDKITKYKQQQMEAKNNDQYRAFLQEIAAAEK
                     AISGLEDRELVIMDQIEAAKKIIAEREAELKEEEGGVSEEKEMLEDRLSEVQEDLNAL
                     LADRGRITATVNHSLLVKYDRLFKNKGDFAVVEVEKDHCSGCHMRLPPQVINDALNPA
                     KLVICNFCGRMLINQR"
     gene            10128..>10347
                     /gene="rnpB"
                     /locus_tag="PKI68_05655"
     ncRNA           10128..>10347
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKI68_05655"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         2542 a         2632 c         2878 g         2295 t
ORIGIN      
        1 ggcgggttgc ttcgggagat catgtgatga aaaaaatgac ccggtttatt tttttattgc
       61 tgggagcctc cgccggttct ctttccgccg ctgttgctcc ccatgtcgga tttgtttatc
      121 cggccggcgg aacacccggg acaactttta ccgttacgat cggcggtcaa tacctgagag
      181 attttaaaga tattcatctt tccaatgttc cggttaaagc ccagctgacc gactatctgc
      241 gtatttatga ctccaaggaa ggcggcgggc tgcgccggaa aaaagaaact cttgaagcca
      301 agatggcgga agcgacggat gcaaggtcta agttgcagtt gcagaaacag attgaaatgc
      361 tggagccgga aatcgctatg gttgcggaag tgcgccggca ggacagaatg aatccttcga
      421 tggctgcaaa aaaacagttt aatccgcaga tttccgagcg gattacgctg gaaattacgc
      481 tgccggcaga cgctaagccc ggggaatatg agttacgagt gattaccacc aatggccttt
      541 cgaatccgct tttatttgag atcgggcggt tgcccgaaat tgctgaagtc gagccgaacg
      601 acctgactaa aaatgcagcg aaattgccgg ttttgccggt gctggtgaac ggtcagatac
      661 tgcctggtga tgtagactca ttccggtttc acgctctcaa agaccagacg cttgttatta
      721 aagcatcagc ccgttctctg gtaccttatc tggcggatgc cgttcccggc tggttccagt
      781 cggtactgac gctctatgac gccaacggca aggaagttgc ctatgacgac gattatcttt
      841 ttaacccgga tcccgttctg atttatcacg tgccggaaac cggcgactat acattttcaa
      901 tccgtgattc gatttaccgc ggacgcgagg attttgtgta ccgtatctcg atcggggagc
      961 ttccgttcat tgaaaggata tttcctttag gcggtccggc cggcagcgaa ctggatgtga
     1021 atctttatgg cgtcaatttg ccgtataaaa aaatcagact caaaacgaat gccgatgcgc
     1081 cggagacgca gtatgttcat gttgaaaaag gcggactcat ctccaacagc cagccttttg
     1141 gcgtatcacc gtttcctgac cgttcggaaa gcgaaccgaa caatcagttt tcagaagccg
     1201 ttgctgtaac gaatgatact gcaatcaacg ggacgatcgg aaaacccggc gaccacgact
     1261 ggttctgttt taaaggacgc aaaggtgaac agaaaaccat tgaggttttt gcccgccggc
     1321 tcggttcacc gatggattcc tgtctgacgc tttttgacac gaatcagcag gtactggcgg
     1381 tgaatgatga tgccgaagat aaaagtttcg ggttgctgac acatcaggcc gattcccgca
     1441 ttgatttcac attgccggaa accggaattt attacgtgag gttggatgac cagcagaata
     1501 aaggcggcga tgcatatgcc taccggctgg tgatcggcaa agaaaagcct gatttccaat
     1561 tgcgggtggt tccctccagc ctgcgtattc cgcgtaacgg tacagcgatt gccaccgttt
     1621 atgccattcg ctacggcggt ttcacagacg cgatcagttg ttcagttcgc gatgcgccga
     1681 cgggaattga gctccagaga gccgtaatac ctgccggaac gaatgtcgcg aagatcgtga
     1741 ttactgccag gccgagggct gcagagcaga tgatgactct tgatatcgaa ggtgttgcag
     1801 actgcggatc gagaactgta cgccgccacg ccgttcctgc agaagatatg atgcaggcct
     1861 ttatataccg ccatctggtg accgcacagc agatgcttgt tcaggtaaca gaacctgagc
     1921 cggtatccgt cgttctaacc attcccaaag aaggattttt ccgcgcccat cccggaaaac
     1981 agctggtaat tagcgcatcc gcccagtggg cggcatctgc ccagaaaagc ataaaactca
     2041 cattggcgga accgcccgaa tggcttacct tgcgaaccgc tacctggcag gccggtaata
     2101 tcattctgga tatcagtccc aatgctgaac ccggtgatac agcaacggtg cttctgaccg
     2161 gaacggtcaa ggttaacaaa tcaccaaaag atgcggactt taatcccgtc atgaaatttt
     2221 tgaacaaccg cacggccgat ttcaccattg atgcaattcc aatacaagtt gttgaatgaa
     2281 aggatgagcc ttgactgcac agatctcaaa aacgaattac gatgccgatg tcgccactct
     2341 cacactgccg tcgcatgccg aacactgcat taccgttcag tccgaacaga atatgaaccg
     2401 ggtgctgagc cgtgccgcgg aggaacggga cgccaccatt ctcagccagt ttgttttcgc
     2461 cggacggcag cattaccctg aatttcttgc ggaaacgggc gacaccggct ggcccgttgt
     2521 ctggctgcag ggcgatgcat gcaaagacgg aagtatgtac tcaatgcagg ctgttgccat
     2581 ttccggcatt aagccgcaac cggtcagact cgccggtcgc gatgtcggct ttacctatga
     2641 agataagcat gcccgttact gtcgtctgcg cggaatcctg cccgccgacc ttaaagcctc
     2701 ccgtgcggcg caaacccatt ccgctttcca gattatggaa gccgctctga aagagaacgg
     2761 cttccgcttc accgacactg tccgcacctg gatatatctc gatcagctcc tcgaatggta
     2821 cggcgagttc aaccgggttc gcacggattt ctttaacgaa aaagacatct tcagtcacat
     2881 ggtgccggcc agtaccggta ttggtgccgc caatccgttt ggcgccgcca tcatgatgga
     2941 tgttctcgca gttcagccga aaaccaaaca gattgaaatt aacgaagtca tttcaccgct
     3001 tcagaatccg gctcttgatt ataagagctc tttcagccgt gccgtcgaaa tgaaatttcc
     3061 gacacaccgc agcctgctga tctccggcac agccagcatc gatccgtccg gcaaaacggc
     3121 atatctggat gatcctgaga aacaaatccg gctcacgatg gatgttgtca aagccatcct
     3181 gagttctcgc gacatgggct ggagcgacct tttccgcgga atcgcctact tcaaagacat
     3241 ggactatctg ccgatttatc ggcgtatcgc tgcggagctg ggtgttcccc gctttccgct
     3301 ggcggtctcg catgccgatg tctgccgaga cgatcttctc ttcgaaatcg aagtggatgc
     3361 cgtcaaagtc cgctgatagt tttattgctg cataagaaaa tgttttaaac tcttctttaa
     3421 atatgcaagt ttctctcatg ccagagaaac gtgtcagtga agatggcggg gcttatcagg
     3481 tgcgggcgaa tgatctgccc aaagcgcttc agccgcgcga aaagttcgat cggctcggtg
     3541 cagaaaatct ctccgacagc gatttgcttg cgctgcttct gcggaccgga accgctggac
     3601 gcaatgttgt cgaactggcc gaagcacttt tgatgcaata cggttcactc agcgcgcttt
     3661 cgcgcacttc agtcgcagaa ctgcagaaaa ttcacgggat cggaaaagag aaagccaaaa
     3721 tcctgaaagc tgcacttgaa atcggacggc ggctggcaca ggaaaatgtc ggcgaaaacc
     3781 cgcgcatcgc ttcgccggaa gaagccgctg cagtcttgcg tgaacgcgcc cgcagtcttg
     3841 accgcgaagt cttctgggtt ctgctgcttg acacaaaaaa ccggctgatg ctgcctccgt
     3901 gcgaagtatc gaaaggaact ctgaactcca gtctggttca tccgcgcgaa atttttaaac
     3961 cggcggttca gaactccgcc gcaagcatta tcctggcgca taatcatccg tcgggagatc
     4021 ctgcgccgtc ggcgcaggat attcgcatca ccagaaagct ggttgaggcc ggaaagacta
     4081 tggaaatcaa ggtgctggat catctcatta tcggccgcaa aacccgtgat ggcgcaaacg
     4141 atttcctcag cctgcgcgaa accggactgg ccgaatttga agaacggtag aaagagcttg
     4201 aacgcatgct ttaaatgaca ataatcgctc ccatcttcag ggcagggtga aattcccgac
     4261 cggcggtgat acattccgag gaagctcgaa aagtaaagtc cgcgagccga aaggcagatg
     4321 cagtgcgatt ctgcaaccga cagtagagtc tggatgaaag aagataaaca gaatgcgaaa
     4381 acctttctgc cctgaaagac ctgtggtctt cagggttttt gcatttaaga atggtgcgat
     4441 atgagtgaaa agctgtttga ttcgatggag gagattcttg acgccgtccg ccgcggcgag
     4501 atggttttga ttacggatga cgaaaaccgc gaaaacgaag gcgacgtaat ctgcgcggcc
     4561 gaattctgca ctccggagca gattaatttt atgatcaccc atgcccgcgg actggtctgc
     4621 gtaccggtca cggcggagcg ggccgcacat ctgggcctga agcggatgaa catttcgcat
     4681 gaaggcgata agttcaaaac ggcatttacg atctcagtcg acgccgctga cagcacgacc
     4741 gggatcagtg cggcggaacg ggcgcatacg gtccgtatgc tggtcgataa aaatacccgg
     4801 cccgaagata tggtctgtcc agggcatatt ttcccgctga tcgcgatgaa tggcggtgta
     4861 ctggagcggg ccggacacac ggaggcgacc attgacctga cccgcatggc aggtcttcag
     4921 ccggccggtg tgatctgtga ggtggcgaac gacgacgggt caatggctcg tctgcccgat
     4981 ttgataaaat tcgcgaagaa acacggactg aaaatgactt cggtcgctga aattgccaaa
     5041 taccggtata ttaccgaaac cctgattcgt atggaacggc aggtgaagat gccgacagac
     5101 tggggcgatt ttgacctgcg gatctacagc tcggtagtgg acgataaaga gcatctggca
     5161 ctgtttaccg ggcatccgac accggaaaag cctcatccgc tggtgcgtgt tcacagcgaa
     5221 tgtctgaccg gcgatgtttt cggatctcag cggtgcgact gcggtgctca gctccagctt
     5281 tcaatgcaga tgatcagcga ctacggctac ggtgctcttg tttatatgcg gcaggaaggc
     5341 cgcggaatcg gtttaaccaa gaaaatccat gcctatgaat tgcaggatca gggtttggat
     5401 acggttgagg ccaatgttcg gcttggtttc gcgccggatc tgcgcgatta tggaatcggc
     5461 gctcagattc tcaaagatct ccatatgacc ggaatccggc tgatcacgaa taatccggcc
     5521 aagattgagg gactggataa atatggcatt gaggttgtcg agcgggtgcc gattgtgctt
     5581 ccttcaacac cgcacaatgc acgctatttg aataccaaac gggaaaaaat gaaacacctg
     5641 ctctgaaaca ggagcgaata tcggagtatc cggaacatgg gaaaaggtat ttcaaaaaca
     5701 gaattgaatg caggcggatt gcggtttgcg atcgtggtca gccggtttaa tgaagaatta
     5761 accagtcagc tggcggacga tgctttccgg tgcctgaaag aaaacggcgc aaaagttccg
     5821 gatatcggaa tttttcgggt gccgggcgcg tatgaaatac cggtgataat caacgagctt
     5881 gccgcaagcg gaaaatatga tgcgttgatt gctttgggcg tagtcgtgga aggcgaaact
     5941 ccgcatgcgc agatgattgg cgaatcaacc ggtattgcgc tgcttgatat tgcctgtaaa
     6001 cacggcgtgc cggtcattaa tgaaattgtc ggtacacgca actgggagca ggcggtagcg
     6061 cgctgcacca gcagccggac gagccgcggc tggtatgccg ccgaggctgc gattgaaacc
     6121 gctaacgttt tgaaaacaat taaaggatct aaatgagtaa agtgatgggt cgccgtcagg
     6181 cgcgcgagtg ggcggtgcag tttctgtttc agaccgagtt caatcccggc gatctggagc
     6241 cggctttgga tcttttctgg aacgatgagg aaaagaagcc gtcggatcgc gaccggacgt
     6301 atgtgaatga agtcgtgcgc ggtgtgattg aaaaacagcg tgaacttgac cggacgattt
     6361 caaaatacac tgacaactgg gacgttgacc gccttggtgt gctcgaccgc atcgtgttac
     6421 gcgttgcgac gttcgaaatg ctttttcgcg gtgatattcc gccggtggtt tcaatcaacg
     6481 aagcagtcga aattgccaaa gcgtacagcg gcaaagattc cggccgcttt gtaaacggcg
     6541 tactcgaccg gattcagaag gaactggacc ggccgtcgcg cagcacctat acgacgcaat
     6601 gagttctcac gagccattca tgcagagggc gctcgcgctg gcaaagcgcg gggaggggct
     6661 gacgcggcct aatccgccgg tcggcgcggt gctggtgcgg aatggaaaaa tcatttccga
     6721 aggctttcat aaaaaagccg gcggtccgca tgccgaggtc aactgcctca aatcagcatc
     6781 cggtaatctt gaatccgcaa ttctctatgt gacgctggag ccctgttcaa cctacggacg
     6841 aacaccgccc tgtactgatc tgattctgtc gcgcggtatc cgttgcgtag tgatcggcgc
     6901 aaaagacccg aatccggctc acgcggggcg gggtatccgg attctgcgcc gcgccggcgt
     6961 tgaggtgatc accggaatat gccggactga ggcggaaacc ctgatcgctc cctttgccaa
     7021 acggattctt accggcatgc cgtatgtgac gctcaaactg gcatcaaccc ttgacgggaa
     7081 gattgccgat gcctcaggaa aatcaaaatg gatcaccggc ccgcaggccc gcgccagggt
     7141 tcaagagctg cgtcgttccg ccgacgcgat tatggttggt gcaggaacgg tgcgcgcaga
     7201 taatccgtcg cttctgccgc gcccggccaa aggccgcgcg ccgtggcggg tgattgtcgg
     7261 aacggatatc cctgaaaaat caaaagtact cacggatgaa gcggccggat gtacgctggt
     7321 gatggacggt tcactcaaaa aaattctgcg cagtcttgga tctcgcggcg tgatgcatgt
     7381 gttgtgcgaa ggcggcggtg aactggccgg agcgctggta cgcgccgggc tggtggatga
     7441 atttgctttg tttgtcgcgc cgtcactgct cggaggtaac ggagttccga tgttcgggaa
     7501 aaaaggatgg ccgctgataa aaatgccgct tttgaatctc caagccttgg aaaaatgcgg
     7561 aaaagatgtt tttatacgag ccttaccggt cagggggaaa tgatgtttac agggattatt
     7621 cagcgtctcg gaacagtggt tgatgtcaaa atgcacggtg ccgccggccg gatcaccttg
     7681 aagccgaacc gtccgtttga ttttcctgtt catctgggcg acagcatcgc cgtaaacgga
     7741 acctgcctga ccgttgccgc gatcgaaaac ggaaatttct gtttcgacgt cctcacggaa
     7801 acgttcaata aaaccaatct cggcgaaaaa ggcccggggg cgattgtgaa tctggagcgc
     7861 gctcttgcgc tgggcgatac gcttggcgga cacattgtga ccggccatgt tgacggcacc
     7921 ggaagagtgc ggcggatcga tcagattgac cgcgactgga aatttgaaat cgaatgtgca
     7981 caagacctgc ttatgctgat ggtgtacaaa ggttcgattg ctatgaatgg aatttctttg
     8041 acggttgcgg aattaaaaga cgacgcgttc gttgttcata ttattccgca tacgctggaa
     8101 gaaactgaca tgtcgcaatt caagccgggt acaaaagtta atctcgaagc cgatattctc
     8161 gggaagcatg tgcagcgtat tctcgaattt ggcggcggac agaattaccg tgtcagtttt
     8221 gagaacaaac agtaagggaa cttcatggac cggaaaaaaa ttctgctggt tgatgacgaa
     8281 gaagccatac gtaaaatggt tcgcgcggtt ctgggcagcg aactctacga attcgctgaa
     8341 gcagccaacg gcattgctgc acaggctata ttggaaaaac agaaattcgc tctggttatt
     8401 tctgacgtaa tcatgcccga ttgtgacggc attgagctgg tgatggccat ccgccgcaaa
     8461 ctgcctgaaa ttaaaatcat tgtcatgtcc ggcggcggcc gcgttcgtgc cggacactat
     8521 ctcgatctgg ccagaaaact cggtgcgacc cgggtttttg aaaaaccttt tgatacggcg
     8581 gaactccgtc aaaccgttca tgacttgctc agctgacagg aaaattatga aagaaaccga
     8641 tctgctcaaa ctcttcgaag atacccaggc gcttctcaac ggccattttg aacttcgttc
     8701 cggcctccat tccaaccgct ttttccagtg tgcgctgctg ttgcagtacc cccggattgc
     8761 cgaacaggtc tgcacggctt tagtcgaaaa gatgaaaacc gtcctcggcg aactggatgt
     8821 ggatactgtg atttcgcccg cgctcggcgg cattccggtc gggcacgaag tcgcccgctc
     8881 tttgggtaaa cgcttcattt tcgcggagaa aaatgcggac aaaatgctga ccatgcgccg
     8941 gtttaagatt gcgcagggcg agcggtttct ggtggcggaa gacgtcatta cccgcggcgg
     9001 ccgtgtgcag gaaacgatcg acatcgtcaa acagcatggc ggcatcgttc attcggtcgg
     9061 cgtactcgtc aaccgcagcg gcggcaaggc gcagtttgac tatccgacgg tcagcctgct
     9121 cgacatcgag ccggtcacct gggaacccgc agaatgcccg ctttgcaggc agggattgcc
     9181 gctggtgcac cccggaagtt agacggtatt ttcggttgtc gaatcggttt ttgggtcgta
     9241 tgctctccgc tcaattttgt tctgctgaat ggagatcata actatggcgc acccgcttga
     9301 agcatttctg gtattacagg aaaaagaccg taaaatcgca aaactccagc gcgaaatccg
     9361 cgacatcccc gcccgcaagg ctgatatcga aaaacagctt gagggctcaa aaaaacggct
     9421 tgccgaagcc cgcgaactgc tgaaaaaagc cacggctgat ctcaagcagc tggaagttga
     9481 agccggagcc catcgcgaca agatcaccaa atataaacag cagcagatgg aggcgaaaaa
     9541 caacgaccag tatcgtgcgt tcctccagga aatcgccgct gccgaaaaag ccatttcagg
     9601 tctcgaagac cgcgaactgg taatcatgga tcagatcgaa gcggctaaaa aaataatcgc
     9661 ggaacgcgaa gccgaactga aagaagaaga aggcggagtc agcgaagaaa aagaaatgct
     9721 cgaagaccgc ctgagcgaag ttcaggaaga cctcaatgct ctccttgccg accgcggccg
     9781 gatcacggca acggttaatc attctctgct ggtgaaatac gaccggctct tcaaaaacaa
     9841 aggcgacttc gccgttgttg aagtcgaaaa agaccattgc tccggctgtc acatgcgcct
     9901 gccgccgcag gtcatcaacg acgctctgaa cccggccaag ctggtcatct gcaacttctg
     9961 cggacgcatg cttatcaacc agcgctaggc ctttaggcgg gacgcgaccg cccgtcctga
    10021 gctccaccga agggcagggc gcgtttagcg tgctcggaga tcactctcca ccttttacag
    10081 aatacttctc gccttggcgg gtgccgatgc gtaccttcct tttccgcagg cagagtgtat
    10141 cggccgctct ttggttcgcc aaagggagga aagtccggac tccgcagggc aggacgttcc
    10201 gtggttaacg cggaaactcc ggtgctaaat cgccggggat ggaaagtgcc acagaaatta
    10261 taccgccctg agggcgcttc ggcgcatcga agggtaaggg tgaaatggtg gggtaaaagc
    10321 ccaccgctcc aagggtaacc gcggagg
//
LOCUS       DAOPZO010000067        10258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_7607_length_10258_cov_7.863472, whole genome shotgun sequence.
ACCESSION   DAOPZO010000067 DAOPZO010000000
VERSION     DAOPZO010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 10258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10258
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7607_length_10258_cov_7.863472"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="PKI68_05660"
     CDS             <1..942
                     /locus_tag="PKI68_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNX53210.1"
                     /translation="QIDEVILVGGSTRMPRVQSSVKHLFDGKEPHKGVNPDEVVAVGA
                     AIQGGIMKGEVKDVLLLDVTPLTLGIETLGGVRTPLIERNTTIPTKKSEIFSTASDSQ
                     PAVDIHVLQGERKMARDNKTIGHFQLDGIPPAPRGVPQIEVTFDIDANGILNVKAKDL
                     GTGKEQHITITASSGLSKEEIEKMMKDAEMHAAEDEKAKERVDLRNQADSVVFQTEKF
                     LKENGDKISADKRAEVEKAIEPVKEALKSDNDDQIKSAMEKLNEVMQAAATEMYSKAQ
                     PGAQEQQQPSGEQPKKEKPADGDVIDADYKMDDDKKK"
     gene            1049..1336
                     /gene="groES"
                     /locus_tag="PKI68_05665"
     CDS             1049..1336
                     /gene="groES"
                     /locus_tag="PKI68_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNX53211.1"
                     /translation="MNIKPLGDRVLVEPIKEAEMKKGGIIIPDTAKERPQEGKVIALG
                     TGKLDENGKVVPFNVKKGDTVLMPKYGGTEVKMGGKEYQIMREEDILAVIG"
     gene            1441..3075
                     /gene="groL"
                     /locus_tag="PKI68_05670"
     CDS             1441..3075
                     /gene="groL"
                     /locus_tag="PKI68_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNX53212.1"
                     /translation="MAKQMKFDVDARDAMLKGVEKLSRAVKVTLGPKGRNVILDKKYG
                     SPNVTKDGVSVAKEIELEDPFENMGAQMVKEVASKTSDIAGDGTTTATVLAEAIYREG
                     LKNVTAGANPMSLKRGIDKAAEALVEALAKMSKKTKSTEELAQVGTISANGDTAIGKI
                     IADAMAKVGEDGPITLEESKSMDTSLDVKEGMLFDKGYLSPYFVTNAEAMEAELEDPY
                     ILLFEKKISNLQDMLPLLQNVAKSSKPLMIIAEDIEGEALATLVVNKLRGTLNVCAVK
                     APGFGDRRKAMMEDIAILTGGKFITEDLGIKLESVTLADLGRAKRVSVSKDETVIVDG
                     AGKKVDINARVDQIKRQIQETTSDYDREKLQERQAKLAGGVAVINIGAATESEMKEKK
                     ARVEDALHATRAAAKEGIVPGGGVALIRAQKALDALKLEGDEAIGVQIVRKACEAPLR
                     QLVDNAGIEGAIVVQEVKKAKGNNGYNVATGEYVDMIAAGIIDPTKVTRSALQNAASI
                     AGLLLTTECMITDLPEKNKPAMPDMGGMGGGMGGMM"
     gene            3173..3433
                     /locus_tag="PKI68_05675"
     CDS             3173..3433
                     /locus_tag="PKI68_05675"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNX53213.1"
                     /translation="MNFEYLTKAEEKIGSIPTLVNLVSYRTRQLIRGERPMVKRDFPE
                     QDFHDVVLKEIVEGKLTVEMGILPIDMSSSFDMFDEGSDIVL"
     gene            3436..3912
                     /gene="smpB"
                     /locus_tag="PKI68_05680"
     CDS             3436..3912
                     /gene="smpB"
                     /locus_tag="PKI68_05680"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNX53214.1"
                     /translation="MSSKVKESRDASGVLATNRKAFRDFHVLEKLEAGIELRGTEVKS
                     IRDGHIRIDEAYAHIENGQAEVLQMTIQPYSHGNVHNHSPTRPRRLLLHKKEIVRLQS
                     KIGEKGLTLVPLKAYLKTGKVKIELALCKGKDTVDKRETLKKKSADLETRRALRGR"
     gene            3920..4345
                     /locus_tag="PKI68_05685"
     CDS             3920..4345
                     /locus_tag="PKI68_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53215.1"
                     /translation="MRSREVMKWEETLKAVFDALDHELETEYGDRYPIHPARPKRGKT
                     ANPEDDGLFNIGAAFSAGFGSKYGPGYVVEIRIATLSRVPHAIREEIKQKIFRRLKER
                     VPAAFPGKKLHVSEENGLIRIHGDLSLDDRAAKSQPQTG"
     gene            complement(4288..4686)
                     /locus_tag="PKI68_05690"
     CDS             complement(4288..4686)
                     /locus_tag="PKI68_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53216.1"
                     /translation="MDRFKRRTFFGGMPPLRAIGFLALMGFALFVSFRIFTAPAKTVQ
                     VIAAPDGSREARLQHVYYASKPGFKVSVREKHLWHTLFYLGEYTNAPAGTVSESLRWS
                     ADSQTLSFEINGQPVWGCDFAARSSRLKSP"
     gene            complement(4686..6077)
                     /gene="rseP"
                     /locus_tag="PKI68_05695"
     CDS             complement(4686..6077)
                     /gene="rseP"
                     /locus_tag="PKI68_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNX53217.1"
                     /translation="MAEFLLNVWTVFYTVFAALFLFGVTVFVHEFGHFIVARRCGLVV
                     KTFSIGFGRAVFKWEKNGILYKIGWIPFGGYVALPQLDPEGMEKIQGDASAERYPEVS
                     PWKKIAVAVSGPLGNIVLAVLLALAIWLIPGDDAGTQIRPVIGTIETNSAAYAAGLRQ
                     GDEITAVNGAAVKNWYDFTVETLLKNKDTAALIVRNGGIERQLDIPVVKGDEGVRTIE
                     GIKPAIPCLFGAVTKDGPADRAGVKSGDKALTFNGMSVLGWEHFTGLVQKVEAGVPSA
                     LIVERKGSPIELSIIPEYSEEYKRIMVGVQLGGGGLPWMLYRNPVDQLKYDALAIVRV
                     LKALVTPSEAKQAAGGLGGPIMIFSMLTMSIKMGVVNTLGLIRFLNINLAVLNLLPIP
                     VLDGGHIVFSLWHGISRRKINIRVQTVLINFFAVLLIGAMLILSFNDLDRKFQIKRFF
                     GRLSGQTEQVEKK"
     gene            complement(6096..6950)
                     /locus_tag="PKI68_05700"
     CDS             complement(6096..6950)
                     /locus_tag="PKI68_05700"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNX53218.1"
                     /translation="MDKKRITLGLIIAAVLSGAMLLVPAGNIAVPLGLMAVCGLAMRE
                     VYALMERASLLASKKTGIAGGLLFVAMTWLVAKYPCPGFSGDDMLWSLLLLILMVIFF
                     RLFGYTDARQALRNALGTLFGFLYVAFFWSFFVRLYTMGNLDEPTRAAFYLLLAVKWG
                     DAGAYFIGSRFGKHKLFPRISPKKSWEGLLGGILFSCVVGVLWCFCTGNTLGQYSFPL
                     YHALILGVLLPLIGTLGDLVESIFKRAVDIKDSGAIAHGMGGMLDMIDSLLFAAPFMY
                     IYIKFFLC"
     gene            complement(6960..7661)
                     /locus_tag="PKI68_05705"
     CDS             complement(6960..7661)
                     /locus_tag="PKI68_05705"
                     /inference="COORDINATES: protein motif:HMM:NF011405.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNX53219.1"
                     /translation="MPQHVAIIMDGNGRWAQQRGLPRIKGHEQGAESVRAVLRAARQA
                     GVKFLTLYAFSTENWVRPKDEVEGLMNLLAIFMDRYEGELLKNKIRLRVMGQIERLPE
                     KVRKKLAKLMEKSAKDYEYTLIVALSYGSRLEIAEAAKAIARKAAKGELDPETVNEET
                     VAQHLYLPDVPDPELMIRTSGELRLSNFLLWQLSYAELYVTDVFWPDFREPEFFQALE
                     AFNQRGRRFGGTVTQ"
     gene            complement(7702..8982)
                     /locus_tag="PKI68_05710"
     CDS             complement(7702..8982)
                     /locus_tag="PKI68_05710"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNX53220.1"
                     /translation="MANTVLIGAQWGDEGKGKVIDVLTANANWVVRYQGGNNAGHTVE
                     IGDQKYVLHLAPSGILRPGVQCVIGNGLVVDPLGLAEELQDLEKRGIDFRGRLFVSDR
                     AHLVIQYHKELDGAKEARLATGKKIGTTKRGIGPAYSDKSDRTGLRMGDVLEADFESM
                     LRERIAAKNSMLQVLGATPLNADELVARYRVAFDYLKPFICDTVPMLNAAVRDPANNV
                     IFEGAQGVMLDIDFGTYPFVTSSSTSSGGVATGAGIPPHCIDDVIGIVKAYTTRVGEG
                     PFPTELLDADGEELRKVGHEFGATTGRPRRCGWFDAVVARYSAMIGGIDRWALMKLDV
                     LDGFETIKVCTAYECDGERVETVPASISKLSRCKPVYEEFKGWNCSTRNVTCFEELPE
                     QAQKYVRWLEKLTGVPVSILSVGPNRASTIELKS"
     gene            9124..9990
                     /locus_tag="PKI68_05715"
     CDS             9124..9990
                     /locus_tag="PKI68_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX53221.1"
                     /translation="MKYPAVIPKTVLVTGCSSGIGLATARLLLERGWQVAPTARKAAD
                     LEMLRAEGFDPIALDVTDSASIEAAADTVLARFGGQLGAVVNNAGFGLTSAIEDAPRA
                     MLRDIFEVNVFGLQELTNRFIPLFRKQGCGRIVNVSSVLGELSLPFAGVYSASKFAVE
                     ALSDAMRRELFDSGVAVSIIQPGPIETKFSENLAHRTERYGLNPDSPFTAFYKETIET
                     RKTGTAHRTAGVFMKPPEAVAEKILNCLESPRPPIRARVTAVAYFGAFVRRALPAALV
                     DAVMISQLKKRI"
     gene            complement(9997..>10258)
                     /locus_tag="PKI68_05720"
     CDS             complement(9997..>10258)
                     /locus_tag="PKI68_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53222.1"
                     /translation="EEQHLSMYDLAGMYADNLIEPPNDVEGLKWMNIVSTCAATFSEG
                     NEGRQYILSDPKNVRKKLESRMTEQQKLTAAQLADQWMNSRN"
BASE COUNT         2527 a         2974 c         2791 g         1966 t
ORIGIN      
        1 cagattgacg aagtgattct ggtcggcggt tcgacccgta tgccgcgcgt tcagagttcg
       61 gtcaaacatc tgttcgacgg caaagagccg cataagggcg tgaacccgga cgaagtggtc
      121 gcggtcggcg cggcgattca gggcggtatt atgaagggcg aagtgaagga tgttctgctg
      181 ctcgacgtta cgccgctgac cctcggcatt gaaacgctcg gcggcgtgcg cactccgctg
      241 atcgaacgca atacgacgat cccgacgaag aagagcgaaa ttttctcgac ggcatccgac
      301 agccagccgg ccgtggatat tcacgttctg cagggcgaac gcaagatggc ccgcgacaat
      361 aagaccatcg ggcacttcca gctcgacggc attccgccgg ctccgcgcgg cgtgccgcag
      421 attgaagtga cgttcgatat cgacgccaac ggcattctga atgtgaaggc caaagacctc
      481 ggcaccggca aggagcagca catcaccatc acggcttcga gcggtctctc caaggaagag
      541 attgagaaga tgatgaagga cgctgaaatg cacgccgccg aagacgaaaa ggccaaagag
      601 cgcgttgatc tgcgcaacca ggcggacagc gtcgtgttcc agacggagaa attcctgaag
      661 gaaaacggcg acaagatttc cgccgacaag agggctgaag ttgaaaaggc catcgagccg
      721 gtcaaggaag cgctgaagag cgataacgac gatcagatca agtcggcgat ggaaaagctg
      781 aatgaagtga tgcaggcggc ggctacggaa atgtattcca aggcccagcc cggcgcacag
      841 gaacagcagc agccgtccgg cgaacagccg aagaaggaaa agccggctga cggcgatgtc
      901 atcgacgccg actacaagat ggacgacgac aagaagaagt aaatttagtt atcggctacc
      961 ggttagcggt cagcaggaac ttcctgcgaa gcccgcaacc ggcggcccgt aacagctaaa
     1021 cgataaccac aacaggagga gtgcatccat gaacattaaa ccgttaggcg accgcgtact
     1081 ggtcgaaccg atcaaagaag ctgaaatgaa aaaaggtggc atcattattc ctgataccgc
     1141 caaagagcgt ccgcaggaag gcaaagtgat tgctctcgga accggcaagc tggatgaaaa
     1201 cggcaaagtg gttccgttca atgtgaaaaa aggcgacacg gttctgatgc cgaaatacgg
     1261 cggaaccgaa gtgaagatgg gcggcaaaga atatcagatc atgcgtgaag aagatattct
     1321 cgccgttatt ggttaacagt tatcagttaa aagttaaaaa ccgggttgct aggacgactg
     1381 ttactggaag ttacaaataa ccagtaacaa ataaccaaaa acccttttcg gagaaaagat
     1441 atggctaaac agatgaaatt cgatgtagat gcccgcgacg cgatgctcaa aggcgtagaa
     1501 aagctcagcc gcgcggtcaa agtcaccctc ggcccgaaag gccgcaacgt gatcctcgac
     1561 aagaaatacg gctcgccgaa cgtcaccaaa gacggcgtgt ccgttgcaaa agagatcgaa
     1621 ctggaagatc cctttgaaaa catgggcgca cagatggtga aagaagtcgc cagcaagacc
     1681 agcgacatcg ccggtgacgg aacaaccacc gcgaccgttc tggccgaagc gatctaccgc
     1741 gaaggtctca agaacgtcac cgccggcgcc aatccgatga gcctcaaacg cggtatcgac
     1801 aaagccgccg aggcgctggt cgaagcgctc gccaaaatga gcaagaagac caaatccacc
     1861 gaagaactcg cccaggtcgg cacgatttcc gccaacggcg acaccgccat cggcaagatc
     1921 attgccgacg cgatggctaa agtcggcgaa gacggtccga tcacgctcga agaatccaag
     1981 tccatggaca ccagcctcga cgtcaaggaa ggcatgctgt tcgacaaggg ttacctctcg
     2041 ccctacttcg tgaccaatgc cgaagcgatg gaagcggaac tggaagaccc ctacattctg
     2101 ctgttcgaaa agaagatttc gaacctgcag gacatgctcc cgctcctcca gaacgtggcc
     2161 aagagcagca agccgctgat gattattgcg gaagacatcg aaggcgaagc gctggcgact
     2221 ctcgtcgtca acaaactgcg cggcaccctg aacgtctgcg ccgttaaagc tccgggcttc
     2281 ggcgaccgcc gcaaagcgat gatggaagat atcgccattc tcacgggcgg caagttcatc
     2341 accgaagacc tcggcatcaa gctcgaaagc gtcaccctgg ccgacctcgg acgcgctaaa
     2401 cgcgtcagcg tcagcaagga tgaaacggtc atcgttgacg gcgccggcaa aaaggtcgat
     2461 atcaacgccc gcgttgatca gattaagcgc cagatccagg aaaccacgtc ggactacgat
     2521 cgtgaaaaac tgcaggaacg tcaggcgaag ctggctggcg gcgtagccgt catcaacatc
     2581 ggcgcggcga ccgaatccga aatgaaggaa aagaaagccc gcgtcgaaga tgctctgcat
     2641 gcaacccgcg cagctgccaa agaaggcatt gtgccgggcg gcggcgtggc cctcatccgc
     2701 gcccagaagg cgctcgacgc cctcaagctc gaaggtgacg aagccatcgg cgtccagatt
     2761 gtccgcaaag cctgcgaagc tccgctccgc cagctggtgg acaatgccgg catcgaaggg
     2821 gccattgtgg tgcaggaagt caaaaaagcc aaaggaaaca acggctacaa cgtcgccacc
     2881 ggcgaatatg tggacatgat cgccgccggc atcatcgacc cgaccaaggt gacccgttca
     2941 gctctgcaga acgcggccag cattgcgggt ctgctgctga cgaccgaatg catgatcacc
     3001 gacctgccgg aaaagaacaa accggctatg cccgatatgg gcggcatggg cggcggcatg
     3061 ggcggcatga tgtaagtcgc tgataaagcc taaattgccg tttgacggca gaaagcgggg
     3121 cgtgtacatt acgccccgct ttttagtttc taatgagagg attggacgca ctatgaactt
     3181 tgaatatctg accaaagcag aagaaaaaat cggaagcatc ccgacgctgg tgaatcttgt
     3241 ttcctaccgt acccgccagc tcatccgcgg tgaacgtccg atggtcaaac gcgactttcc
     3301 ggagcaggac tttcacgatg tcgtcctcaa ggaaattgtc gaaggcaaac tcaccgtcga
     3361 aatgggcatc ctgccgatcg atatgagctc ctcgtttgac atgttcgacg aaggttccga
     3421 tatcgtcctt taaaaatgtc ttccaaagtc aaagaatccc gggatgcttc cggcgtgctg
     3481 gccaccaacc gcaaagcgtt ccgggatttt cacgtcctgg aaaagctgga agccggcatt
     3541 gagctgcgcg gcacggaagt caaatccatc cgcgacggtc acatccgcat cgacgaagcc
     3601 tatgctcaca tcgaaaacgg acaggccgaa gttctccaga tgaccattca gccctattcg
     3661 cacggcaacg ttcacaacca cagtccgacc cgtccgcgcc gcctgctcct gcacaaaaaa
     3721 gagatcgtcc gcctccagag taaaatcggc gaaaaggggc tgacgcttgt gccgctcaag
     3781 gcctatctca aaaccggcaa agtaaaaatc gaactggcgc tttgcaaagg caaagacacg
     3841 gtcgacaaac gcgaaacgct caaaaagaaa tccgctgacc ttgaaacccg ccgggcgctg
     3901 cgcggacgct gagttcgtca tgagaagccg cgaagtcatg aaatgggaag agactctcaa
     3961 ggcggtcttt gatgcgctgg atcacgagct ggaaaccgaa tacggtgatc gttatcccat
     4021 ccaccctgcc cgaccgaagc ggggaaagac cgccaatccg gaagatgacg gtctgttcaa
     4081 catcggcgcg gccttttccg ccggcttcgg gtcaaaatac ggcccgggct atgtcgtcga
     4141 aatccgcatt gcaacactga gccgggttcc acacgccatc cgcgaagaaa taaaacagaa
     4201 aattttccgg cggctgaaag aacgggttcc ggcggccttt cccggaaaaa agcttcatgt
     4261 ctcggaagaa aacgggctga tccgtattca cggcgatttg agcctggacg accgtgcagc
     4321 gaaatcgcag ccccagaccg gctgaccgtt gatttcgaac gagagcgttt gagaatccgc
     4381 gctccagcgc aacgactcgc tcaccgttcc tgccggtgca ttggtgtatt cccccagata
     4441 gaacagcgta tgccagaggt gtttttcgcg caccgagact ttaaaccccg gctttgaggc
     4501 atagtaaacg tgctgcaggc gtgcttcgcg gctgccgtcg ggcgccgcga taacctgtac
     4561 ggttttcgcc ggtgcggtaa aaatccggaa cgagacaaac agcgcaaagc ccatcagtgc
     4621 cagaaagccg atggcgcgca gcggcggcat gccgccgaaa aaagtccgcc tcttaaatcg
     4681 atccattatt tcttttcaac ctgctccgtc tgaccggaca gccgtccgaa gaaccttttg
     4741 atctgaaact tacgatccag atcgttgaag gaaaggatga gcatggcgcc gatcagcaga
     4801 acagcaaaaa agttgatcag caccgtctga acccggatgt tgattttgcg gcggctgatg
     4861 ccgtgccaga gcgaaaagac aatgtgcccg ccatctaaaa ccggaatcgg cagcagattc
     4921 aaaaccgcca gattgatatt gagaaagcgg atcaggccga gcgtattcac aacccccatt
     4981 ttaatcgaca tggtaagcat actgaaaatc atgatcggtc cgccgaggcc gcccgccgcc
     5041 tgtttagctt cggaaggcgt aaccagcgct ttaagcaccc ggacgatggc cagcgcatca
     5101 tatttgagct gatcaaccgg gttgcggtaa agcatccacg gcagtccgcc gccgccgagc
     5161 tgcactccga ccatgatccg tttatattct tcgctgtatt ccgggatgat tgaaagttcg
     5221 atggggctgc ctttgcgttc gacaatgagg gcggacggaa cgccggcctc aaccttttgc
     5281 accagcccgg tgaaatgctc ccacccaagc actgacatgc cgttgaatgt cagtgctttg
     5341 tcgccgcttt tcacgccggc gcgatccgca ggaccgtctt tggttacagc gccgaacagg
     5401 cagggaatgg cgggcttaat accttcaatc gtacggaccc cttcatcgcc tttgacaacc
     5461 gggatatcca gctgccgctc aatcccgccg ttacgcacaa taagcgcggc agtgtctttg
     5521 tttttaagca gcgtttcgac ggtgaagtcg taccagtttt taacggcagc gccgttgacc
     5581 gcagtaatct catcgccctg gcgcaggccg gcggcgtagg ccgcgctgtt ggtttcaata
     5641 gtcccgatga ccggtcggat ttgtgtgccg gcatcgtcgc cgggaatcag ccagatggcg
     5701 agcgccagca gaacggccag cacaatgttg ccgagcggac cggaaacggc gacggcgatt
     5761 tttttccacg gagaaacttc cggatatctt tccgccgagg catcgccctg gattttttcc
     5821 ataccctcgg gatcgagctg cggcagcgcg acgtaaccgc cgaacggaat ccagccgatc
     5881 ttgtagagaa tgccgttctt ctcccattta aaaacggcgc ggccgaaacc gatggagaac
     5941 gttttgacga ccagtccgca gcggcgcgcc acgatgaagt ggccgaattc gtggacgaaa
     6001 acggttacac cgaaaaggaa cagcgctgcg aagacggtgt agaaaacagt ccagacattc
     6061 agcaggaatt cagccattga gaaatatccg cctttctagc acagaaaaaa tttgatgtag
     6121 atgtacataa agggggcggc aaaaagcagg ctgtcgatca tatcgagcat accgcccatt
     6181 ccatgcgcga tcgcaccgga atctttaata tccaccgcgc gcttgaagat agattccacg
     6241 agatcgccca gagtcccgat gagcggaagc aggacaccga gaatcagcgc atgataaaga
     6301 ggaaatgaat attgcccgag cgtgtttccg gtgcagaaac accagagtac gccgacgaca
     6361 caggaaaaaa gaatgccgcc gagcaaccct tcccagctct ttttcggaga gatgcgcgga
     6421 aacagtttgt gcttcccgaa gcgtgaaccg atgaagtaag cgcccgcatc tccccattta
     6481 accgccagca gcagataaaa ggcggcccgg gtgggttcat caaggtttcc catcgtgtac
     6541 aggcggacaa aaaagctcca gaaaaacgcc acataaagaa acccgaaaag agtgcccagt
     6601 gcgttgcgaa gcgcctgccg cgcgtcggtg taaccgaata gccggaaaaa aataaccatc
     6661 agaatgagca gcagcaagga ccagagcatg tcatcgccgg aaaatccagg acacggatat
     6721 ttggcgacca gccaggtcat ggcgacaaaa aggagcccgc cggcgattcc cgttttcttc
     6781 gaggcaagca ggctcgccct ctccatcagc gcatacactt cacgcatggc cagcccgcag
     6841 accgccatca gacccaacgg caccgcaata tttccggccg gcaccagcag catcgcgccc
     6901 gaaagcaccg ccgcaataat cagccccagc gtgattcttt ttttatccat aacagtcccc
     6961 tactgcgtca ccgttccgcc gaagcggcgt ccgcgctgat taaaagcttc caaagcctga
     7021 aaaaattccg gttcacggaa atcgggccag aacacatccg tcacatacag ctcggcatac
     7081 gaaagctgcc agagcagaaa attactcaac cgcagttcgc cgctggtgcg gatcatcagc
     7141 tccggatcgg gcacgtcggg cagatagaga tgctgtgcca cggtttcttc atttaccgtt
     7201 tccggatcga gctcgccctt cgccgccttg cgcgcaatgg ctttggcggc ttcggcgatt
     7261 tcgagccggc tgccgtagct cagcgcaaca atcagcgtgt attcgtaatc cttcgccgac
     7321 ttctccatca gcttcgccag cttcttccga accttctcgg gcaaacgctc gatctgaccc
     7381 atcacccgaa ggcggatttt attcttcagc agctcgcctt catagcgatc cataaaaatc
     7441 gccagcagat tcatcagtcc ttcgacttca tccttcggac ggacccagtt ttcggtactg
     7501 aacgcataaa gcgtcagaaa tttaacacct gcctgccgcg ccgcgcggag taccgcgcgg
     7561 accgattcag cgccctgctc gtggccctta atccgcggca gaccgcgctg ctgcgcccag
     7621 cgcccgttgc cgtccataat aatggcaacg tgctgcggca cttttgcaag tgttggtact
     7681 tcagctttca ggtttccgcc ctcagctttt caattcaatc gtactggcac ggttcggacc
     7741 gacggacaga atgctaacgg gcacaccggt cagtttttcc agccagcgga catatttctg
     7801 cgcctgttcc ggcagctctt caaagcaggt cacgttgcgg gtcgagcagt tccagccctt
     7861 gaactcttca tagaccggtt tgcagcgcga aagtttgctg atgctggccg gtacggtttc
     7921 gacgcgctcg ccgtcgcact catacgcggt gcagactttg atcgtttcaa aaccgtcgag
     7981 tacgtcgagt ttcatcagcg cccagcggtc gatgccgccg atcatcgcgg aatagcgtgc
     8041 gacgactgcg tcaaaccagc cgcagcggcg cggacgaccc gtggtggcgc cgaattcgtg
     8101 gccgactttc cgcagctctt cgccgtcggc gtcgagaagt tcggtcggga acgggccttc
     8161 gccgacgcgt gtggtgtagg ctttgacaat accgattaca tcgtcgatgc agtgcggcgg
     8221 aatgcccgcg ccggtagcca cgccgccgga gctggtggat gaggaggtga cgaaggggta
     8281 tgtgccgaaa tcaatgtcca gcataacgcc ctgcgcgcct tcaaaaatca cattgtttgc
     8341 cggatcgcgc acggcggcgt taagcatcgg aaccgtgtcg cagatgaacg gtttgagata
     8401 atcaaacgcc acgcggtatc tggcaaccag ttcgtcggca ttcagcggag tcgcgccgag
     8461 aacctgcagc atggagtttt tggctgcgat gcgctcacgc agcatcgatt caaaatcagc
     8521 ctccagcaca tcgcccatcc gcagaccggt gcggtcggat ttatcggaat acgccgggcc
     8581 gatgccgcgc ttggtcgtgc cgatcttttt accggtcgcc agcctggcct ctttcgcgcc
     8641 gtcgagttcc ttgtggtact gaatcaccag atgggcgcga tcactgacaa acagccgccc
     8701 gcgaaaatca attccgcgct tttcgaggtc ttgcagttct tcggccagac cgagcggatc
     8761 gaccaccaga ccgttgccga tcacacactg tacgccgggg cggagaatgc ctgacggagc
     8821 cagatggagg acatatttct ggtcgccgat ttccaccgta tgaccggcat tgttgccgcc
     8881 ctgatagcga acgacccagt tcgcgttcgc ggtcagaaca tcaatgacct tgcctttgcc
     8941 ttcatcgccc cactgcgcgc cgattaaaac tgtatttgcc atgcgtaaaa tcccttgatt
     9001 ccgggttgcg tctcaaaggc gcagagagta gcccaccgct ccggaggggt caatttccaa
     9061 acccagcaag aaatggttcc aatgttcagg caaaaaaggc atctttccgc cactggagat
     9121 cctatgaaat atcctgcggt aatcccgaaa actgtactcg ttaccggctg ctcgtccgga
     9181 atcggactgg cgacggcgcg ccttttgctc gaacgcggct ggcaggtcgc cccgaccgcc
     9241 cgcaaagccg ccgacctcga aatgctccgc gccgaaggct ttgacccgat cgcgctcgac
     9301 gtcaccgaca gcgcctccat cgaggcggcg gcggacacgg tactcgcccg cttcggcgga
     9361 cagctcggtg ccgtcgtcaa caacgcgggc ttcggactga cttccgccat cgaagacgcg
     9421 ccgcgcgcca tgctccgcga tattttcgag gtcaacgtct ttggattgca ggaactgacc
     9481 aaccgcttca ttcctctttt ccgtaaacaa ggctgcggcc gcatcgtcaa cgtaagctcc
     9541 gtgctgggcg aactgagcct gccgttcgcc ggagtctatt ccgcgtcaaa atttgccgtt
     9601 gaagcgcttt ccgacgccat gcgccgcgaa ctgttcgatt ccggcgtcgc tgtttccatt
     9661 attcagccgg ggccgattga aacgaagttt tccgaaaatc tggcgcaccg taccgaacgc
     9721 tacggactga atcccgattc gccgttcaca gcgttttata aggaaacgat tgaaacccgc
     9781 aaaaccggca ctgcgcaccg cacagcaggt gtatttatga aaccgccgga agccgtcgcg
     9841 gagaaaattt taaactgcct ggaaagtccg cgtccgccga tccgtgcccg cgtcaccgcc
     9901 gtcgcttatt ttggagcctt cgtccgccgc gccctgcccg ccgcgctggt tgatgcggtt
     9961 atgatctccc agctgaaaaa gcgaatataa ccgcagttaa ttgcgactgt tcatccactg
    10021 atctgcaagt tgtgccgccg tcagtttttg ctgttcagtc attcgtgatt caagcttctt
    10081 tcttacattc ttcggatcgc tcaagatata ttgcctgcct tcatttcctt ctgaaaaagt
    10141 cgctgcacaa gtagaaacaa tattcatcca ttttagtcct tcgacatcat tgggcggctc
    10201 gatcaaatta tcagcataca tgcccgccag atcatacatg gatagatgct gctcttct
//
LOCUS       DAOPZO010000068        10230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_7641_length_10230_cov_8.178968, whole genome shotgun sequence.
ACCESSION   DAOPZO010000068 DAOPZO010000000
VERSION     DAOPZO010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 10230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10230
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7641_length_10230_cov_8.178968"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="PKI68_05725"
     CDS             complement(<1..1157)
                     /locus_tag="PKI68_05725"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNX53223.1"
                     /translation="MKTKWITVLTAALLAAGLPVQAQENLISLSFEDAPLEQAVELYR
                     EWTGRTVIKQADLSARITLKGRQLTQNEAKQAIETVLSMNGVALVPMGDKFLKVVPIA
                     NARQEGMEIKPFDPDKKYAAADQLITQVIPLRFVVFADVQPLIQHLVHGYGKIIEMAR
                     INSVLITDTSANIAKIVELVSMVDQPAEKIEPRIYQLKYAEAATIAGKLKELVEAAKT
                     KKGEQTVVANDTRVIPGVIRAPRQETGAAAVAEQPAEQTEMIVGEVKMVADDRTNILI
                     IFSKAANFGFFDNIIKVLDVPVDPAVVVEVVNLEFAKAKEIAGILNEFVGAAKATEKT
                     KAPGAGKVEGSKTVDEVIQKAVETTGVELSPAAKNAIGRLSADTKILSDERS"
     gene            complement(1320..1853)
                     /locus_tag="PKI68_05730"
     CDS             complement(1320..1853)
                     /locus_tag="PKI68_05730"
                     /inference="COORDINATES: protein motif:HMM:NF022201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HNX53224.1"
                     /translation="MKISKRELILCVVALTAILSGFTYWLAGSKIAEQRRIKEDKVRL
                     LRQIELHKRILNEKGSWYSRLEELQAQLPVYDDKTAVSAELPKLIKRMADEHGLDLVR
                     TQTEQEKQSGSLYELAVSCNWEGSLSALVHFLYSLHSQGLRYDVLQLTAQPDAQRERI
                     LKGSMLIECAYRKGKSE"
     gene            complement(1850..3253)
                     /gene="pilM"
                     /locus_tag="PKI68_05735"
     CDS             complement(1850..3253)
                     /gene="pilM"
                     /locus_tag="PKI68_05735"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNX53225.1"
                     /translation="MMLFNHTTGIHYTDEKVEWAVLRRSRKGIEKLREGSMPVPAGFF
                     EQENAPPFPSDVLAHIHKNFRGIVTVSLPSSQLLMRMLELPSTNPAELKGMVELQMDQ
                     ISPFPVDQLTVSYEVLRQTENHSRVLAVAAQRKTVDALGELFKAQNVYIRSLDAEVLA
                     WWSLLIAHGEVLCQGRVVLILEEHTEFSMIVVDEGVPVCFRSLELFHNFTDEPVMREI
                     VEEVRYTLLSLEAEYGRREITGVLFWSESEIPPLLCDLLREECRAEVVLHDLRSIPPV
                     SEGLALRTAERRLHHVELVPREWIELQRRKRLMKVATIASIAVLSVWLSVITIAGAVF
                     SFQQAAYNRVRKEAEKYAGPARAAQSAREEMLSLEKYSDRSHSALECLREVTVALPVN
                     VEITSFTYKKGEAVSLRGSSELADNVYDFFQKLGASDIFTGVKDQPVSTRTVKDRKIS
                     TFSVTAELPKTAKEVRP"
     gene            complement(3250..4257)
                     /locus_tag="PKI68_05740"
     CDS             complement(3250..4257)
                     /locus_tag="PKI68_05740"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HNX53226.1"
                     /translation="MKREAGNLKFGNRRGSALIVVMWILVIVAMIVSSFAFEMQLESR
                     IISAQRKRFKADQLALAGVEMAKAMLSVKKDANKPDKETLSEDPYLNESAKLSEALPV
                     EYREAFGDGEVTVKIDYEEGRLNVHTLSDEGWKLLFDQAGIPNTRWDAMLDCLKDWQD
                     ENDLHQLNGAESDDPFYKKRGYECKNAPVDTVDELLLIKNWGEEVLYGTPPGEEPDAP
                     IAGIAKQLTTWGSGKINPNSASTQVLNGLNIPDSTIDAIQEMRLGPDGVAGTKDDGIS
                     AADFTAMGLSPDIFTLTPEYASVTAIGTVGGVQSKISCIFKLGEKEAVPLFWIEGKTT
                     E"
     gene            complement(4262..5020)
                     /locus_tag="PKI68_05745"
     CDS             complement(4262..5020)
                     /locus_tag="PKI68_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNX53227.1"
                     /translation="MGVSVCVCAGAERNCSSSTHTLTNSHSPTHPPRLRCGFTLLEIL
                     ISMVILLIAMTVAWQTFSTATRAWKGGRETLDKVHHGDFVIGQTASALRSMAFFKSKP
                     EKYAFRIENNRSGYGEHTISWVTGSSAFIPPGEAVSHGLHRIEVGGGEDEDGNEGLLV
                     TIWPYLADEEKVEKQSWLVSENIKGLSCKVYDTKEGEEGWKDTWEYSNAIPGLVEITL
                     YAEPVEKNDEPVKFRQIIEIPLGPLVTNDISAAK"
     gene            complement(5029..5430)
                     /locus_tag="PKI68_05750"
     CDS             complement(5029..5430)
                     /locus_tag="PKI68_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNX53228.1"
                     /translation="MSVISRQFKNGMTLIEVLLAVAILGVGAGVLMMATARCLAVISK
                     SRHYSTAQRLILQVGAEHPLTRGSIKAESDSGRFDDAPDYSWERQITEPEDENRKGLY
                     TVRTRVSWSDRGRKSFEEVVTWHYIPPEVEQ"
     gene            complement(5427..5933)
                     /locus_tag="PKI68_05755"
     CDS             complement(5427..5933)
                     /locus_tag="PKI68_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNX53229.1"
                     /translation="MKDIPAIGNRQSAISNGFTLIEILLVVVIILIATSVSVPLFRGT
                     FQSTQITDAVRTTVRIARYARSIAVIKQEECTLRFKDGLVTISCGSGTNSTEPEISRR
                     LPGDIKVSAFENMAESTKHRDEDRMVRYYPSGMNDGFKLTLSSADDRRKTILCNPISG
                     KTTVEDGR"
     gene            complement(5987..6373)
                     /locus_tag="PKI68_05760"
     CDS             complement(5987..6373)
                     /locus_tag="PKI68_05760"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HNX53230.1"
                     /translation="MKDKKNRKSRSGFTLIEILLVVVIIGIIVGIAVPKMSGKIGMSL
                     DVAARASLKSLETAVQNYEMENLRLPDSLEDLTVVKDGKGPYLKPSEINDPWKQKFQY
                     AKPGSHNRGYDLYTKSPEGKEFNNWD"
     gene            complement(6395..7636)
                     /locus_tag="PKI68_05765"
     CDS             complement(6395..7636)
                     /locus_tag="PKI68_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNX53231.1"
                     /translation="MPTFKYIARGADGKRVDGTLIAADRRAALQMLQQAGHTPLSIDQ
                     IAEKVSTASVKKRFKFESGLRHKTQMGARDTLLFTREMADLLSSGMTLGRALHTLAQR
                     KDESVQSQIVKRLRDEVVQGSSLSDALRIYPDTFQPLYISMVRAGEASGALADSLAGL
                     CSHYERVQEARSKVITAMIYPAIVLGVGMISLIGLMVFIIPRFASIFDELGGTLPLPT
                     RILMAMSNGMIHYGWLMLILLAAGGITLRRWMLTPAGKLWWNEKQLKLPIVRKITTAN
                     AFAHFARTLETLIRNGVPILKALVIVQETVGNEVIAKEIAAARDRVTDGSSVSGPLAA
                     GKVFPPLLTDMLAVGEETGDLPGALKHIARRYDAELDQSIKVLTTVLEPVLILGVAVI
                     VGFVAISMLTAVFDLTSGLGV"
     gene            7710..8282
                     /locus_tag="PKI68_05770"
     CDS             7710..8282
                     /locus_tag="PKI68_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53232.1"
                     /translation="MRLITLFTVGLVLTFAGCSKAPQEKAITLVNAAAVDAPLLERIR
                     AFAQNELHVPVRAVENPKLAGKPDFQALQKAARRVKTNADVSLIVLAGFNGEPRHLTV
                     YSADGIALINAQALYTADAETFARRIERQVMRAAAFSFGLTPTPDPFCVTHDYNSLEE
                     LDAMGRNFSPPWQGRFAEEAAKRGLVPVEK"
     gene            complement(8303..8956)
                     /gene="nth"
                     /locus_tag="PKI68_05775"
     CDS             complement(8303..8956)
                     /gene="nth"
                     /locus_tag="PKI68_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNX53233.1"
                     /translation="MKTKKARADFIGKRLEELYPKVEVPLKHTDAYTLLVAVILSAQC
                     TDKRVNEVTPALFAKAGTPQEMVQLGQEKIRSLITTCGLANNKSRAIYEMSKILVAEH
                     GGRVPDSFAALEKLPGVGHKTASVIMAQAFGVPAFPVDTHIHRLAQRWKLTSGKSVEQ
                     TEADLKKLFPPERWNKLHIQLILYGREHCTARGCDGTRCEICRTLFPDRTQPVKSRK"
     gene            9045..10031
                     /gene="mvaD"
                     /locus_tag="PKI68_05780"
     CDS             9045..10031
                     /gene="mvaD"
                     /locus_tag="PKI68_05780"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HNX53234.1"
                     /translation="MTRQEFVKVLLQRAAGKPGTRGEAFAPANIALCKYWGKRDSELN
                     LPVNSSLSISLGKLGTHTVVKFCKTADRIFLNGRPAPADFAARLSKYLDLFRAEGQHF
                     EVRTKNNIPTAAGLASSASGFAALVKALDQLFDWGFNPRELSMLARLGSGSASRSLYD
                     GFAVWHAGVRADGMDSYAEGFADDWNELRIGVLEISKSKKKTGSTDGMNRTVETSELY
                     EAWPAQADCDFDELRTAIAAQDFQMLGKTAENNALAMHATMLAAWPPLCYWKPQSVAL
                     MQKVWQAREDGLELYFTMDAGPNLKLLFLEKDVRAVRKRFPKIQIVTPFSKA"
BASE COUNT         2035 a         2875 c         2908 g         2412 t
ORIGIN      
        1 gagcgttcgt cggaaagaat cttggtgtcg gcggagagtc ggccgatggc gtttttggct
       61 gccgggctga gttctacgcc cgttgtttcg acagcttttt gaatcacttc gtcaacggtc
      121 ttgctgcctt ccactttacc ggcgccgggc gctttggttt tttcggtggc tttggccgcg
      181 ccgacaaatt cattcagaat cccggcaatt tctttggctt tggcgaattc cagattgacg
      241 acttcgacga cgacggccgg gtccacgggg acgtcgagta ctttgatgat gttgtcgaaa
      301 aagccgaagt tggcggcttt tgaaaagatg atgaggatgt tggtgcggtc gtcggcaacc
      361 attttgactt cgccgacgat catttcggtc tgttccgccg gctgttcggc gacggccgcg
      421 gcgccggttt cctgccgcgg cgcgcgaatt acgccgggaa tgacgcgtgt gtcattggcc
      481 acgacagtct gttcgccttt ttttgttttg gcggcttcga ccagttcttt gagttttccg
      541 gcaatggtgg ccgcttcggc gtatttgagc tggtagatgc gcggttcaat tttttccgcg
      601 ggctggtcca ccatgctgac gagttcgacg atcttggcga tgttggcgga ggtgtcggtg
      661 atcagaacgc tgttaatccg cgccatttcg atgatttttc cgtagccgtg gacgaggtgc
      721 tgaatgagcg gctggacgtc ggcgaagacg acaaagcgga gcggaatgac ctgggtgatg
      781 agctggtcgg ccgccgcata ttttttgtcc gggtcaaacg gtttgatttc catgccttcc
      841 tgccgggcgt tggcgatcgg cacgactttg aggaatttat cgcccatcgg cacgagggcg
      901 acgccgttca tcgagagaac ggtttcaatg gcctgtttgg cttcgttctg cgtgagctgg
      961 cggcctttga gggtgattcg tgcgctgagg tcggcctgtt tgatgaccgt gcgtccggtc
     1021 cattcgcgat acagctctac ggcctgttcg agcggagcgt cttcaaagct gagtgatatg
     1081 aggttttcct gcgcctgtac aggcagtccg gcggcgagca gggcggcggt tagaacagta
     1141 atccattttg ttttcatagc tgttgtcctt ttaaaattcg aaacaggaaa ctgatttaga
     1201 aaaaacccac cccgccgttc cggcacccct ccgcaggagg ggattgttca gccgcaatat
     1261 tcccctccac cggaggggcg gccgcaggcc ggggtgggtt gctgggttcc gggttcccat
     1321 tattcggatt ttcctttccg gtaggcgcat tcgataagca tggagccttt caggatgcgt
     1381 tcgcgctggg cgtcgggctg ggcggtgagc tggagaacgt catagcgcag gccctgactg
     1441 tggaggctgt acagaaaatg gacgagcgcg ctaagcgagc cttcccagtt gcagctgaca
     1501 gccagttcgt aaagcgagcc gctctgtttt tcctgctcgg tctgtgtccg cacaaggtcg
     1561 agcccgtgtt cgtccgccat gcgtttgatc agtttcggca gttcggcgct gacggcggtt
     1621 ttatcgtcgt ataccggaag ctgggcctga agttcttcaa ggcggctgta ccagcttcct
     1681 ttttcattca gaatgcgttt gtgcagttca atctggcgca gcaggcggac tttatcttcc
     1741 ttgatacggc gctgttcggc gattttggag ccggccagcc agtaggtgaa cccggaaaga
     1801 attgccgtga gtgcgactac gcacaggatc agttcccgtt ttgatatttt catggccgga
     1861 cctccttggc ggtcttcggg agttcagccg tgacggaaaa ggtagaaatt ttccggtctt
     1921 ttacggtgcg ggtgctgacc ggctggtctt tcacgcctgt aaaaatatcc gaggcgccga
     1981 gcttctggaa aaagtcatac acgttgtcgg ccagttcgct ggagccgcgc aggctgacgg
     2041 cttcaccttt tttataagtg aaagaggtga tttcgacgtt tacaggcagg gcgacggtga
     2101 cttcccgcag gcattcaaga gcggagtgcg agcggtcgga atatttttcg agcgagagca
     2161 tttcctcgcg tgcggactgt gcggcgcggg cagggcctgc atatttttca gcctctttgc
     2221 ggacgcggtt ataggccgcc tgctggaatg aaaacaccgc gccggcgatg gtgatgaccg
     2281 acagccagac gctgagtacc gcgatggagg caatggtggc gactttcatc agcctcttgc
     2341 ggcgctgcag ctcgatccat tcgcgcggta cgagttcaac gtggtgcagc cgccgttctg
     2401 cggtacgcag cgccagtcct tcggagaccg gcggaatgga ccggaggtcg tgcagtacaa
     2461 cctcagcgcg gcattcttcg cgcagcaggt cgcaaagcag cggaggaatc tccgattcgc
     2521 tccagaagag tacgccggtt atttcccggc ggccgtattc ggcttcaagc gagagcaggg
     2581 tgtagcgcac ttcttcgacg atttcacgca tgacgggttc gtcggtgaaa ttatggaaaa
     2641 gctccagcga gcggaaacag accggcaccc cttcatccac gacgatcatg gaaaactcgg
     2701 tgtgctcttc gagaatgagt accacgcggc cctggcagag cacctcgccg tgagcaatca
     2761 gcaggctcca ccacgccagc acttcggcgt cgaggctgcg gatgtagaca ttctgcgctt
     2821 tgaagagctc gccgagcgca tcaaccgttt tgcgctgtgc ggccaccgcc agaacgcgcg
     2881 agtggttttc cgtctggcgg agcacttcgt aggaaaccgt cagctggtca accgggaagg
     2941 gcgagatctg atccatctgc agttcgacca tccccttgag ttctgcagga ttcgtcgagg
     3001 gcagttcaag catccgcatc aaaagctgcg aagacgggag cgaaaccgtt acgatgccgc
     3061 ggaaattttt atggatatgt gcaagcacgt cggacgggaa aggcggagcg ttttcctgtt
     3121 cgaaaaaccc cgccggaacc ggcatggagc cttcccgaag tttttcaatg cctttgcggc
     3181 ttcggcgcag cacggcccac tcgacctttt cgtcggtgta gtgaatgccg gttgtatgat
     3241 tgaacagcat cattctgtcg tttttccttc gatccagaat aagggaacgg cctccttttc
     3301 gcccagctta aagatgcagg agattttact ctgaacgccg ccgacggtac cgatggctgt
     3361 gaccgaggcg tactccggtg tgagagtgaa aatatccgga ctcagtccca tcgccgtgaa
     3421 atccgccgcg ctgattccgt catcctttgt gccggctact ccgtcgggac cgaggcgcat
     3481 ttcctgaatg gcgtcaatcg tactgtcagg aatgttgaga ccgttgagta cttgcgtgct
     3541 ggcgctgttg gggttgattt ttcctgaacc ccacgttgta agctgtttgg caattccggc
     3601 aatcggagcg tcgggttcct cgccgggcgg ggttccatag agcacttctt cgccccagtt
     3661 tttgatcagc agcagttcgt ccacggtatc aaccggtgcg tttttgcatt cgtaaccgcg
     3721 tttcttgtaa aaggggtcgt ccgactcagc gccgttaagc tggtggagat cgttttcatc
     3781 ctgccagtcc ttcaggcagt cgagcatggc gtcccagcgg gtgttcggaa taccggcctg
     3841 gtcgaacagc agtttccagc cctcgtcact cagtgtatga acgttcagcc ggccttcctc
     3901 ataatcgatt ttgacagtaa cttcgccgtc gccgaatgct tcgcggtatt caaccggcag
     3961 agcctcggaa agtttggctg actcattcag gtaaggatct tcggaaaggg tttctttatc
     4021 cggcttgttg gcatctttct taaccgaaag catcgctttg gccatctcga cgcccgccag
     4081 cgccagctgg tcggccttaa agcgcttgcg ctgtgcggaa atgatgcgcg actccagctg
     4141 catttcgaac gcgaacgaac tcacaatcat ggcaacgatc accaggatcc acatcaccac
     4201 aatcagcgcc gaaccgcgcc tgtttccgaa tttcagattt ccggcttcac gtttcataac
     4261 ttcacttcgc tgcagaaata tcattggtga cgagcgggcc tagcgggatt tcgatgatct
     4321 ggcggaattt gaccggctcg tcattttttt cgaccggctc ggcgtagagg gtgatttcga
     4381 ccaggcccgg aatcgcgttg ctgtattccc acgtatcctt ccagccttct tcgccttctt
     4441 tggtgtcata cactttgcag ctcagtccct tgatattttc gctgaccagc cagctctgtt
     4501 tttcgacttt ttcttcgtcc gccagatagg gccagatggt aacgagaagt ccctcgttgc
     4561 catcctcatc ttcgccaccg ccgacttcga tgcggtgcag gccgtgcgag accgcttcgc
     4621 cgggcgggat gaacgcgctg ctgccggtta cccagctgat cgtatgttcg ccgtaaccgg
     4681 agcggttgtt ttcaatccgg aaggcgtact tctccggctt ggacttaaaa aacgccatag
     4741 agcggagtgc tgacgccgtc tggccgatga caaaatcgcc gtgatgaact ttatccagcg
     4801 tttcgcgtcc gcctttccat gcgcgtgttg ccgttgaaaa cgtctgccac gccacagtca
     4861 tggcgattaa cagaatcacc atggagatca atatttcaag gagagtgaag ccgcaccgca
     4921 ggcgcggggg atgcgttgga gaatgcgagt tcgtgagtgt gtgagtactg gaagaacagt
     4981 ttctttccgc tccggcacac acacacacac ttacccccat actcgttttc actgctccac
     5041 ctccggcgga atgtagtgcc aggtgacgac ttcctcgaag ctcttccgtc cgcggtctga
     5101 ccagcttact ctcgtcctca ccgtatagag tcctttgcgg ttttcatcct ccggttcggt
     5161 gatctggcgt tcccagctgt agtcaggggc atcatcgaaa cggccggaat cgctttcggc
     5221 tttgatggag ccccgcgtca gcggatgttc cgcgccgact tgcagaataa gacgttgcgc
     5281 ggtgctgtag tgccgcgact tggaaataac ggccagacag cgcgcggtgg ccatcatcag
     5341 cacgcctgcg ccgacgccga gaatcgccac tgccagcagc acttcgatca gcgtcatgcc
     5401 gtttttaaac tgtcttgaaa tgacactcac cgtccatcct ccacggtggt tttccctgaa
     5461 atcggattgc agaggatggt tttccgtcgg tcatctgcgc tgctgagcgt cagcttgaag
     5521 ccgtcgttca tgccggacgg gtagtaccgc accatgcggt cttcatcgcg gtgcttcgtt
     5581 gattcagcca tattttcaaa tgcgctgact ttaatgtcgc ccggcagccg gcgggaaatt
     5641 tccggttctg tactgttggt tccgctgccg caggaaatgg tcaccaggcc gtccttgaag
     5701 cgaagcgtac attcttcctg tttgatgacc gcgatactgc gtgcgtagcg ggcaatccgg
     5761 acggttgtgc ggactgcatc ggttatctgc gtcgactgaa aggtgccccg gaacagcgga
     5821 acagaaaccg acgtggcgat cagaatgatc accaccacca gcagaatctc aatcagcgta
     5881 aagccatttg agattgccga ttgccgattg ccgattgccg ggatgtcttt catgttttcc
     5941 agccgatctt caatctcaaa tcgtcaatcg gaaatccgaa atgttcttag tcccagttat
     6001 tgaactcttt gccttcgggc gacttggtat aaaggtcgta gccgcggttg tggctgccgg
     6061 gtttggcata ctgaaatttc tgtttccacg gatcgttgat ttcgctgggc ttcagataag
     6121 gccctttgcc gtctttaacc acggtcaggt cttcgaggct gtccggcaga cgcagatttt
     6181 ccatttcata gttctgcacg gcggtttcaa gactcttgag gctggcgcgt gccgccacat
     6241 ccagcgacat cccgatcttt ccggacattt tcggaacggc gatgccgacg atgatgccga
     6301 tgatgaccac caccagcaga atttcaatca gggtaaagcc gcttctggat ttgcgatttt
     6361 ttttatcttt cataattcat ccctcatctt tctgttagac gcccagtccg cttgtcagat
     6421 caaataccgc ggtcagcata ctgatcgcca caaagcccac gatgaccgcg acgcccagaa
     6481 tcaaaaccgg ctccagtacg gtcgtcagca ctttaatgct ctggtcgagt tcggcgtcat
     6541 aacgccgcgc gatgtgtttt aaagcgccgg gcaggtcgcc ggtttcttcg ccgacagcca
     6601 gcatatccgt cagcagcggc ggaaacactt tgcctgccgc cagcgggccg gaaacactcg
     6661 atccgtcggt tacgcggtcg cgcgcggcgg caatttcctt tgcaatcacc tcgttgccga
     6721 ccgtttcctg aacgatcacc agcgccttga gaatcggcac gccgttgcgg atcagggttt
     6781 caagagtgcg cgcaaaatgg gcgaacgcgt tggcggtggt gatcttgcgg acaatcggca
     6841 gcttgagctg cttttcgttc caccagagtt ttccggccgg cgtcagcatc cagcggcgga
     6901 gcgttatgcc gccagcggcc agcaggatca gcatcagcca gccgtagtga atcatgccgt
     6961 tgctcatcgc catcagaatg cgcgtgggca ggggcagtgt accgccgagc tcatcaaaaa
     7021 tgctggcaaa acgcggtatg atgaacacca tcagcccgat cagagaaatc atgccgacgc
     7081 cgagcacaat cgccggatag atcatggcgg tgatgacttt actgcgcgcc tcctgcacgc
     7141 gttcgtagtg gctgcacagc cctgcaagcg aatcggccag tgcgcctgaa gcttcgccgg
     7201 cccgcaccat gctgatgtac agaggctgaa acgtatcggg ataaatccgg agcgcgtcgg
     7261 acagagagct tccctgaaca acttcgtcgc gcagccgttt aacaatctgt gattgaaccg
     7321 actcgtcttt gcgctgggcc agtgtatgaa gagcccggcc cagtgtcatg ccggaagaaa
     7381 gcagatcggc catttcacgg gtgaacaaca gggtgtcgcg cgcacccatt tgggtcttat
     7441 ggcggaggcc ggattcaaac ttaaaacgtt ttttgacgga tgccgtgctg actttttcag
     7501 caatctggtc gatggaaagc ggcgtatggc ctgcctgctg gagcatttgc agcgccgcac
     7561 gccggtcggc ggcaatcagt gtgccgtcca cccgtttacc gtctgctccg cgcgcgatat
     7621 atttgaaagt aggcataaaa cagatttgaa aacagcgttc agaaaacggc aggatactag
     7681 ccgcaattcg gggataaaag gagtttttta tgcgtcttat aacactgttc acagttggac
     7741 tggttctgac attcgccgga tgctccaaag cgccgcagga aaaagccatc accctcgtca
     7801 acgccgccgc cgtcgatgct ccgctgctgg aacgcatacg ggcttttgca cagaatgagc
     7861 tgcatgtccc ggtgcgcgcg gttgaaaatc ccaaactggc cggtaagccg gattttcagg
     7921 cgcttcaaaa agccgcgcgc cgtgtaaaaa cgaatgccga cgtttctctg atcgtactgg
     7981 ccgggtttaa cggcgagccg cgtcatctga cggtctattc ggcagacggc atcgcactga
     8041 tcaatgctca ggctctttat actgcggatg ctgaaacctt tgcccgccgc atcgagcggc
     8101 aggtgatgcg cgcggcggcc ttcagtttcg gcctgacgcc gacgcccgat ccgttctgcg
     8161 tcactcatga ctacaactca ctcgaagaac tcgacgcaat ggggcgcaac ttttcgccgc
     8221 cgtggcaggg acgcttcgcc gaagaggccg cgaaacgcgg gcttgttccg gttgaaaaat
     8281 aaaatcagag gttcctctga gttcactttc tggatttgac cggttgggtc cgatccggga
     8341 aaagcgtccg gcagatttcg cagcgggtgc cgtcgcagcc gcgcgctgta cagtgttccc
     8401 gtccgtaaag aatcagctgg atatgcagct tattccaccg ttccggcggg aacagctttt
     8461 tcaaatcggc ttcggtctgc tcaacacttt taccgctcgt cagcttccag cgctgcgcga
     8521 gacggtgaat gtgcgtatcc accgggaacg caggaacgcc gaacgcctgc gccataatca
     8581 ccgaagccgt cttatgcccg actccgggaa gcttttccaa tgccgcaaaa ctgtccggta
     8641 ccctgccgcc gtgttcggcg acgaggattt tcgacatttc ataaatcgct cgggacttat
     8701 tgttcgccag cccgcaggtc gtaatcagcg aacggatctt ttcctggccg agctgcacca
     8761 tttcctgcgg tgtgccggcc ttggcaaaca gcgcgggcgt tacctcattg acccgcttat
     8821 cggtgcactg cgcggacagg atgacggcga ccagcagcgt ataggcatcg gtgtgtttca
     8881 gcggaacctc cactttcgga tacagttcct ccagccgctt accgataaaa tccgcccgcg
     8941 cctttttcgt tttcattgaa gatattttta cagaagggaa cgaaggaaac gaagaggata
     9001 tctaaacctt tgttcctgga gaggccttct gttagagtcc cggcatgaca cgtcaggaat
     9061 tcgttaaagt tctgctccag cgggccgcag gcaagcccgg aacccgcggc gaagcgttcg
     9121 cacccgccaa catcgcgctg tgcaaatact ggggcaagcg cgattccgag ctcaacctgc
     9181 cggtcaactc gagcctttcg atttcactcg gaaagctcgg cacgcatacc gtcgttaaat
     9241 tttgcaaaac cgccgaccgc attttcctga acggccggcc ggcccccgcg gattttgccg
     9301 cgcggctttc gaaatacctc gacctgttcc gcgcggaagg acagcacttc gaagtccgca
     9361 ccaaaaacaa tataccgacc gccgccggac tggcgtcgtc ggcttccggc ttcgccgcgc
     9421 tggttaaggc tctcgatcag ctgttcgact ggggattcaa tccgcgcgaa ctttccatgc
     9481 tggcgcggct cggcagcggc agcgcatccc gctcgctcta cgacggcttt gccgtctggc
     9541 acgccggcgt tcgcgccgac ggcatggaca gctatgccga aggctttgcg gacgactgga
     9601 acgaactgcg catcggcgtt ctggaaattt ccaagtcgaa gaagaaaacc ggttcgaccg
     9661 acggcatgaa ccgaacggtt gaaacgtctg agctgtatga agcctggcct gcacaggccg
     9721 actgcgactt tgacgaattg cgtactgcga ttgccgcgca ggattttcaa atgcttggca
     9781 aaaccgccga gaacaacgcg ctggccatgc acgccaccat gctcgcggcg tggccgccgc
     9841 tctgctactg gaagccgcag tccgttgccc tcatgcagaa agtctggcag gcgcgcgaag
     9901 acgggctgga actctatttc accatggacg ccggcccgaa cctgaaactg ctctttctcg
     9961 aaaaagatgt ccgcgctgtg cggaaacgtt tcccgaagat tcaaatagtt acccctttct
    10021 ctaaagcctg atgtctatag cctaaagcct tccccctatg cctgaacttc cggaagttga
    10081 aaccattcgc cgccagctct gcgccgccgg catagaaggg cgcatcatcg aaaaagttca
    10141 tcttgaatgg ccgcgcaccg tcgagccgct gacgccggac gctttctgta aagaagtgaa
    10201 gggacgtaaa atcgaaaaga ttgatcgcca
//
LOCUS       DAOPZO010000069         9905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_8003_length_9905_cov_10.589848, whole genome shotgun sequence.
ACCESSION   DAOPZO010000069 DAOPZO010000000
VERSION     DAOPZO010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 9905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9905
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8003_length_9905_cov_10.589848"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PKI68_05785"
     CDS             <1..435
                     /locus_tag="PKI68_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53235.1"
                     /translation="PFLGRYPTIFQGINAAICYISPPITAVFVFGVFWKKASSFGSIT
                     TLCTGSAIGFIIFLLDFMKEKTGWNVPFMMSGFYLFCICAVIMVIASLMRPEELTEAR
                     RALIWNHPLDALKDKGWPGLGNYKFLAALLFVVMIVLYTVFH"
     gene            455..1222
                     /locus_tag="PKI68_05790"
     CDS             455..1222
                     /locus_tag="PKI68_05790"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: protein motif:HMM:NF017174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HNX53236.1"
                     /translation="MKFKILIALYVAMALSSFGFSPYENYLLSGQQTHVMLFAEVKGG
                     QSNAVQNAVKTLSGKEAQSALSLANISNVSAYTKELQGKLCVMVYFDYDGKNYLDAVK
                     AFEATAQTKALTALVQPHPRAAATGTSWLQMEWVNYIYGAKPNGNTPNRFAMVTRLKP
                     EKEVEYRLLHQSVWPGTVDRMTRMDYHDFSIFFVEMGSELYEMFYVEFIGTDAGKNKM
                     GSVADPTYERWVKNTDPCQIPLPDASGIWSMMNKISE"
     gene            1245..2492
                     /locus_tag="PKI68_05795"
     CDS             1245..2492
                     /locus_tag="PKI68_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNX53237.1"
                     /translation="MTSRERVLAAINHKQPDRVPVDMGSNPSSGISAMGYNKLKAGLN
                     IRGGHTRIYDVVQQLAQPEDEVLSALGVDVIDIGRAFNDSDADWYDITMADGSTAQYP
                     KWFHPTLRADGSYLVERDGVSIARMPPGGAFFDQEYFPYLKGYPQDYSRLPAEMNKIL
                     WQALAHSPWDHASDATFWADLRAKALHLRQTTDKALMIVCGCNLFEWGTFLRRMDNFL
                     MDILAEPEEVEKLLDALMELHLKGLEKVCNAVGDVCDILRFGDDLGMTGGLFMPPDAY
                     RELFKPRHTQLNAYVHKHSNMKTFLHSCGSIYRVMPDLIEAGYDIINPVQTNCFEMDP
                     ATLKKEFGADITFWGGGCDTAAVLNKATPAEVRKHVLERCEIFAPGGGFVFNTIHNIL
                     PEVPPENVIAAFNAVKEFNGDNV"
     gene            2666..3991
                     /locus_tag="PKI68_05800"
     CDS             2666..3991
                     /locus_tag="PKI68_05800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX53238.1"
                     /translation="MKVVHIVPGSGGTFYCQNCMRDAALVQALRRQGIDVVMVPMYLP
                     MFVDGSPVQKHTPVFFGGINVWLQQQFPVFRKTPRWFDRLFDSKWMLRRAAAMEGTTS
                     AAGLGAMTLSMLEGSDGNQRKEVDRLVQWLTGHEKPDAVHISNALLIGLAGEIKAALA
                     VPVVCTLQDEDEWLDKIDPPFNRACRDAIAARCKDVDRFVSVSGWFADRMSERLKIAR
                     DRIDVVHIGIDLNGYEPAALDLNPPVLGYLSRMNETSGLGRLVDAFIELKKTRGLENL
                     KLRATGGAVGSDKKYISGLQKKLAKSGLGNDAEFIEDFSREQRIAFLKSLSVLCVPAE
                     QGEAFGTYIIEALAAGVPVVQPDAGAFPELIAATGGGVIYQNLAETLREVLTHPDHLR
                     QLGRTGRASVMEKFSVETMAQKMISVYMKLAVPAPGTAGFRQYCSKAGT"
     gene            4165..5253
                     /locus_tag="PKI68_05805"
     CDS             4165..5253
                     /locus_tag="PKI68_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNX53239.1"
                     /translation="MWSSGAAYAEWPTYHGEPDLRGVSETTLPAAPQMLWRYQADGAI
                     CNTPVSDGERIFFATKKGDVIALNLTGSEVWQKNFMRTNDAGAQVPMQFDAPPASFGG
                     LVLAGTLRGSLIALDARTGAEKWRYETGGTILGSPNQISTNQIAVINQAEGSLHCLEL
                     GSGKLLWKTEGVERCDGSPGVGDGRIVFGSCAAALHIYSAADGKHLKDIEVGGDAQIA
                     GGAAVVGNLVFAGARDGRMLCADAEAGDIVWSSDGSKKQTFSTPAVTQDKVVYSSDNG
                     RVYAVTREDGRLLWTFESGGLPVSPVAAGDKVAVSADGVLYLLSLADGRKLWSQEISD
                     DISSPALIGGMLAVGADDGTVTAFGKKE"
     gene            5258..5926
                     /locus_tag="PKI68_05810"
     CDS             5258..5926
                     /locus_tag="PKI68_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX53240.1"
                     /translation="MSALLKLTDISKRYDEKTPVLCGAGLTVAHGESVAITGPSGCGK
                     STLLNIIGTLERPDAGTLKFAGTDLLTLNEKATAKFRNRKIGFVFQLHHLLPQCSILE
                     NVLVPALVNKAAGDAKRRALHLLERVGLSDRLNHRPGQLSGGERQRAAVVRALINQPQ
                     LLLADEPTGSLSRDGADNLVQLLLELNREENLTLIMVTHSMRLAERMDRVLTLDAGVL
                     KPER"
     gene            5923..9072
                     /locus_tag="PKI68_05815"
     CDS             5923..9072
                     /locus_tag="PKI68_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNX53241.1"
                     /translation="MKPEAARLLIRSLRFYRRSHAGVFLGTVLAAAVLTGSLLVGDSV
                     HGSLRRFALQRLGGIRFAMYTPNRFFSRGLAEKIPDSSAALQLRGMAITDGRQVNRVQ
                     VLGTDSNFWNFAGLNVQLQENETALNQKLAAALGVQVGDEISLRIEKPGLLPGDAPLA
                     SQKNDRTVRGRFTVQRILSDDELGRFSLSANQVVPYNAFVNLHSLADRTELAGKANLL
                     LTGAGDSGDTLKKVWTAEDFGFRFRQQGATVQLESGQVYLEPEAVRAALSIEGAEGTL
                     TYLVNSISKAQKSTPYSFVIARDGGGLRDDEILINRWLADKLSAKPGDPVTMNYFELL
                     PSGRFEVRDRIFTVRRIIEMSELSAERELAPQFPGLTDVDRCADWDVGIPMEKAQLED
                     KANEEYWNSYRQTPKAVVSLKAGQSMWANRFGGLTSVRWPQGAAAAEEFRNRFDPSAA
                     GFIFQPVREQALAAADNAMDFGQLFIGMSFFLIVAALMLTGLLFVFGVQQRAEEMGIL
                     LATGWKPFAVRNLFFMEGGAIALTGSAAGAWLGTGYTKLLILGLATYWQGAVANSAIQ
                     YFARPGTVVAGAAVSFICAILSMGIAMWRQTGHPARELLTADFSQPPLPRLRRARGAG
                     AFAALACALGAVGMVVYALTADVQSVTMPFFGAGALLLIAGVLFCGIALRRAGRRGNL
                     SGIAGMALRNAARRRGRSLTVIGLLACGCFLVFTVSSMKEDVTAHADQPWSGTGGFRW
                     FGESTLPVTDNPGGVRLRVRDGDDAGCLNLNRARSPRLLGVDPDVMSRRKAFLAGDDV
                     WQLLKLELPDGAVPALAGDADTAMWGLEAKTGVEQGALLDYRDEAGNVFKVKLVGRLP
                     MRLSVFQGTLLMAEQRFVEKFPGEAGYRMFLFDDQAGGTAAAKKYARDGMDVVPATQR
                     LMEFYAVESTYLAMFLLLGALGLAVGSMGMGVAVLRNVQDRRTEIALLRAVGYRSRTL
                     RKMLFIEHGLLFAAGLGVGVIASAVAMVPALVISTSQVSALFLFGLLAAVGICGAVCM
                     IAAVSVSLRGDALRGLRNE"
     gene            9253..>9905
                     /locus_tag="PKI68_05820"
     CDS             9253..>9905
                     /locus_tag="PKI68_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX53242.1"
                     /translation="MKVLLVEDEAKIAGFVRKGLEAQGFSVTVSGDGSEAFELAVADS
                     FDVILLDIMLPGRDGLSILKELRERRNTVPVILLTARAEMNERVEGLNLGADDYITKP
                     FFMDELTARIKAVVRRTSGETLSVLHSGPLTLNLITRHAQYGDEEIIFAPREFSLLEH
                     LMRSPGMVFTRDQLLEQVWGYGFDPQTNLVEVCIRRIREKIDRDGNQFIETVRGVGYR
                     "
BASE COUNT         1994 a         2783 c         3109 g         2019 t
ORIGIN      
        1 ccgttcctgg gccgctatcc gaccattttc cagggcatca acgcggccat ctgctacatc
       61 tcgccgccga ttaccgccgt ctttgtcttc ggcgtgttct ggaaaaaagc ctcttccttc
      121 ggctccatca caacgctgtg cacaggatct gccatcggct tcatcatctt cctgctggac
      181 ttcatgaagg aaaaaaccgg ctggaacgtc ccgttcatga tgtccgggtt ttacctcttc
      241 tgcatctgtg ccgtcatcat ggtgatcgcc tcgctgatgc gtccggaaga gctgacggaa
      301 gcccgccgcg cgctgatttg gaatcatccg ctggatgcgc tgaaagacaa aggctggccg
      361 ggtctcggaa actacaaatt cctggccgct ctgctgttcg ttgtcatgat cgttctctac
      421 actgttttcc actaatcgaa aggaaggtcg cgttatgaaa ttcaaaatcc tgatcgctct
      481 ctatgtggcc atggcgttga gctctttcgg cttctcgccc tatgaaaact atttgctctc
      541 cggacagcag acccatgtca tgctgttcgc cgaagttaaa ggcggacagt ccaacgccgt
      601 tcagaatgcg gtaaaaacgc tctcggggaa agaagcccag agcgctctca gtctggcgaa
      661 catctcgaat gtcagcgcct acaccaagga gctgcagggc aaactgtgcg tcatggttta
      721 cttcgactac gacggtaaaa actatctgga cgccgtcaaa gccttcgaag cgaccgcgca
      781 gaccaaggcc ctcaccgcgc tcgtgcagcc gcaccctcgc gccgccgcca ccggcaccag
      841 ctggctgcaa atggaatggg taaactacat ctacggcgca aaaccgaatg gcaacacgcc
      901 taaccgcttc gccatggtca cccggctcaa acccgaaaaa gaagtggaat accggctgct
      961 gcaccagtcc gtctggcccg gcacggtgga ccgcatgacc cgcatggact atcacgactt
     1021 ctccatcttc tttgtggaaa tgggcagcga actctatgaa atgttctatg tcgaattcat
     1081 cggcaccgat gccggcaaaa acaaaatggg cagcgtggcc gatccgacct atgaacgctg
     1141 ggtgaagaac accgacccct gccagattcc tctgcccgat gccagcggca tctggtcgat
     1201 gatgaacaaa atttccgaat aaatccttca cagaaagccc ccttatgact tcacgcgaac
     1261 gtgttctagc cgccatcaac cacaagcagc ccgaccgggt gccggtcgat atgggctcca
     1321 accccagctc cggcatttcc gccatgggct acaacaagct caaagcgggg ctcaacatcc
     1381 ggggcggaca tacccggatc tacgacgtcg tgcagcagct cgctcagccg gaagacgaag
     1441 tcctctccgc gctcggcgtc gatgtcatcg acatcggccg ggccttcaac gactccgatg
     1501 ccgactggta cgacatcacc atggccgacg gctccacggc acagtatccc aaatggttcc
     1561 atccgacgct ccgggccgac ggctcctatc tggtcgagcg cgacggtgta tccatcgccc
     1621 gcatgccgcc cggcggcgca ttcttcgacc aggaatactt tccctatctc aaaggctatc
     1681 cgcaggacta cagccggctg cccgctgaaa tgaacaaaat tctctggcag gcgctggctc
     1741 acagcccgtg ggatcatgcg tcagacgcca ccttctgggc cgacctgcgc gccaaggcgc
     1801 ttcacctgcg ccagaccacc gataaagcgc tgatgatcgt ctgcggctgc aacctcttcg
     1861 aatggggaac cttcctgcgc cgcatggata acttcctgat ggacattctg gccgagcccg
     1921 aagaagtcga aaaactgctc gacgcgctga tggaactgca cctcaaagga ctcgaaaaag
     1981 tctgtaatgc cgtcggcgac gtctgcgaca tcctgcgctt cggcgacgac ctcgggatga
     2041 ccggcggact cttcatgccg ccggacgcct accgcgaact tttcaaaccg cggcataccc
     2101 agctcaacgc ctatgtgcat aagcacagca acatgaaaac cttcctgcac tcctgcggct
     2161 ccatctaccg ggtgatgccg gacctgattg aagcaggcta cgacatcatc aatccggtcc
     2221 agaccaactg cttcgaaatg gatccggcca cgctgaaaaa agaattcggc gccgacatca
     2281 ccttctgggg cggcggatgc gacacggccg cggtgctgaa caaagccacg ccggccgaag
     2341 tccgcaaaca cgtccttgag cggtgcgaaa tcttcgcgcc gggcggtgga tttgtgttca
     2401 acaccattca caacatcctg cccgaagttc ccccggaaaa cgtcatcgcc gcctttaacg
     2461 ccgtcaaaga attcaacggc gacaacgtgt agctccgcag agaccggaaa aacaaaggcc
     2521 ggacaggttt ctgtccggcc ttttttgctg ataaaaacaa aaaggaggcg tcctgtcttt
     2581 taaccttttt ccccggcgcg gcggaaaaca atctggacag gattccctgt tacggttcat
     2641 tctgtgggcc ttcgcccact gaaaaatgaa agtcgtccac atagttcccg gttccggcgg
     2701 tacgttttac tgtcagaact gcatgcgcga cgcggcgctg gtgcaggcgc tgcggcggca
     2761 gggaattgat gtggtgatgg tgccgatgta tctgccgatg tttgtggacg gcagtccggt
     2821 gcagaagcat acgccggttt ttttcggcgg catcaacgtc tggcttcaac agcagtttcc
     2881 ggttttccgc aagacaccgc gctggttcga ccggctgttc gattcgaaat ggatgctgcg
     2941 ccgtgccgct gccatggaag gtacgacttc ggcggccggg ctcggcgcga tgacgctttc
     3001 gatgctggaa ggatcggacg gcaatcagcg caaggaggtc gaccggctgg tgcagtggct
     3061 gacggggcac gaaaagccgg acgcggtgca tatctcaaac gctctgctga tcggactggc
     3121 gggcgagatc aaagcggctc tggcggttcc ggtggtttgc acgttgcagg atgaagatga
     3181 atggctggat aaaattgatc cgccgttcaa ccgggcgtgc cgggatgcga ttgcggcgcg
     3241 ctgcaaggat gtggaccggt tcgtttccgt aagcggctgg tttgcggacc ggatgagcga
     3301 gaggctgaag atcgcgcgcg accggattga tgtcgtgcat atcggcatcg acctgaacgg
     3361 atacgaaccg gcggcgcttg atttgaatcc gccggtgctg ggttatcttt cgcgcatgaa
     3421 tgaaacgtcc ggactcggcc ggctggtcga tgcctttatc gaactgaaga aaaccagggg
     3481 gctcgaaaac ctcaaactgc gcgcgaccgg cggcgcggtc ggctcagata aaaagtatat
     3541 ttccggtctt cagaaaaaac tggcgaagtc cggcctggga aacgatgcgg agtttatcga
     3601 agacttcagc cgcgaacagc ggatcgcttt tctgaagtcg ctgtcggtgc tgtgcgttcc
     3661 ggcggaacag ggcgaagcgt tcggcaccta tatcatcgaa gcgctggcgg caggcgttcc
     3721 ggtggtgcag ccggatgccg gcgcgtttcc tgaactgatc gcggcgacag gcggcggagt
     3781 gatttatcag aatctagcgg aaacgctgcg ggaagttctg actcatcctg atcatctccg
     3841 gcagcttggc cggacgggcc gcgcatcggt gatggaaaaa ttcagtgtcg agaccatggc
     3901 gcagaagatg atttcggttt acatgaaact tgccgtaccc gcgccgggta cggctgggtt
     3961 ccggcagtat tgcagtaaag ccggaactta gggcgaagcc cgcagatccg gcttgaaaaa
     4021 cggacgggcg gacgtggaat aagaggagtg atgaccgcga acaaaaacaa atcagaaact
     4081 gaccgcaaag acgcaaagac gcaaagattc tatggccttc gtgtcttggc gtcttcgcgg
     4141 tcatacatcg cggctgtttt gctgatgtgg agttccggtg cggcttatgc agaatggccg
     4201 acgtatcatg gcgagccgga tttgcgcggc gtttcggaaa caacgctgcc ggcagcgccg
     4261 cagatgctgt ggcgttatca ggcagatggt gcgatttgca acacgccggt gtcggacggt
     4321 gaacggattt tctttgcgac gaaaaagggc gatgtcatcg ctctcaacct gacgggctcc
     4381 gaagtctggc agaaaaattt tatgcggacg aatgatgccg gcgcgcaagt gccgatgcag
     4441 ttcgatgcgc cgccggccag tttcggcgga ctggtgctgg cgggaacatt gcgcggttcg
     4501 ctgattgcgc tggatgcccg aaccggcgcg gaaaagtggc gctacgaaac cggcggcacg
     4561 attctcggat cgccgaatca gatcagcacg aatcagattg cggtaatcaa tcaggcggaa
     4621 ggttcgctgc attgtctgga actcggcagc ggaaagctgc tgtggaaaac ggaaggtgtt
     4681 gagcggtgcg acggttcgcc gggagtcggg gacggccgga ttgttttcgg cagctgcgcc
     4741 gccgcgctcc acatctattc ggcggcggac gggaagcatt tgaaagatat cgaggtcggc
     4801 ggcgacgccc agatcgcggg cggcgcggcc gttgtcggta atcttgtttt tgccggcgcg
     4861 cgcgacggcc ggatgctttg cgccgatgcg gaggcgggcg atattgtctg gagcagcgac
     4921 gggtcgaaga aacagacttt ttcaactccg gcggtgacgc aggataaagt ggtttactct
     4981 tccgacaacg gccgcgtcta tgcggtgacc cgcgaagacg ggcgcctgct gtggacgttt
     5041 gaaagcggcg gactgccggt ctcgccggtc gcggcgggag ataaagtggc ggtttcggcg
     5101 gatggagttc tttatctgct gagtctggcg gacggccgga aactgtggtc gcaggaaatc
     5161 agcgacgaca tcagttctcc ggcgctgatc ggcggtatgc tggcggttgg cgcggatgac
     5221 ggcacggtga cggcgttcgg gaagaaggaa tagacatatg agtgcactgc tgaagctgac
     5281 ggatatttca aagcggtatg acgaaaaaac gccggtgctg tgcggcgccg gacttaccgt
     5341 tgcgcatggc gaaagcgtgg cgattaccgg gccgtccggc tgcggcaaga gtacactgct
     5401 gaacattatc ggaacactgg aacggccgga tgccgggacg ctgaagtttg ccggaaccga
     5461 cctgctgacg ctgaatgaaa aggcgacagc gaaattccgt aaccggaaaa tcgggtttgt
     5521 ctttcagctg catcatctgc ttccgcaatg ctcgattctg gaaaatgtgc tggttccggc
     5581 gctggtaaac aaagccgccg gcgatgcgaa aaggcgcgcc ttgcatctgc tggaacgggt
     5641 cggtttgtcg gaccggctga accatcgtcc gggacagctg tccggtggag aacgtcagcg
     5701 cgcggcggtg gtgcgcgcgc tgatcaatca gccgcagctg ctgctggcgg acgagccgac
     5761 cggttcgctg agccgcgacg gcgcggataa tctggtgcag ctgctgctgg agctcaaccg
     5821 cgaagaaaat ctgaccttga tcatggtgac gcattcgatg cggctggcag aaagaatgga
     5881 ccgggtgctg acgctggacg ccggagtatt aaaacctgaa agatgaaacc tgaagcagcc
     5941 agacttctga tccgcagtct gcggttttac cggcgcagcc acgccggtgt atttctggga
     6001 accgtgctgg cggcggctgt gctgaccggc tcgctgctcg tcggcgattc ggtacacgga
     6061 tcgctgcgca ggtttgcgct gcagcggctc ggcggaatcc ggtttgcgat gtacactccg
     6121 aaccggtttt tctcgcgcgg actggcggaa aaaattccgg acagttcggc ggcgcttcaa
     6181 ctgcgcggca tggccatcac agacggcagg caagtgaatc gtgttcaggt gctcggaacc
     6241 gattccaact tctggaactt tgcggggctg aacgttcagc ttcaggaaaa tgaaacggcg
     6301 ctgaatcaga agctcgccgc cgcgctcggc gttcaggtcg gcgacgaaat ttctctgcgc
     6361 atcgaaaagc ccggactgct gccgggcgat gcaccgcttg cttcacagaa aaacgaccgg
     6421 accgtgcgcg ggcggttcac cgttcagcgg attctcagtg acgatgagct gggccgcttc
     6481 agtctgtcgg cgaatcaggt ggttccgtat aacgcctttg tgaatctgca cagtctggcg
     6541 gaccggacgg aactcgccgg caaagccaat ctgctgctga cgggcgcagg cgattccggc
     6601 gatacactca aaaaagtctg gacggccgag gacttcggct tccggttccg gcagcagggc
     6661 gcgacagtac agctggagtc cggtcaggtt tatctcgaac cggaagcggt gcgcgcggcg
     6721 ctgtcgattg aaggcgccga aggaacactg acctatctgg tgaattccat ttccaaagcg
     6781 cagaagtcca cgccgtattc ttttgtaatc gccagggacg gcggcggact gcgcgacgat
     6841 gaaatcctga tcaaccgctg gctggcagac aaactcagcg cgaagccggg cgatccggtg
     6901 acgatgaact actttgaact gctgccttcc ggccggtttg aagttcgcga ccggattttt
     6961 acggtccgcc ggattatcga gatgagcgag ctgtccgccg agcgcgagct ggctccgcag
     7021 tttcccggtc tgacggatgt ggatcgctgt gcggactggg atgtcgggat tccgatggag
     7081 aaagcgcagc tcgaagacaa ggcgaacgag gagtactgga acagctaccg gcagacgccg
     7141 aaagccgttg tctcgctgaa agccggacag tccatgtggg ccaaccggtt cggcggtctg
     7201 acgtcggtac gctggccgca gggcgccgcg gcggcagagg agttccggaa ccggtttgat
     7261 ccgtccgccg ccggatttat ttttcagccg gtacgcgaac aggcgctggc agccgccgac
     7321 aacgcaatgg acttcggcca gctgtttatc ggtatgagtt tttttctgat cgtcgcggcg
     7381 ctgatgttga ccggtctgct gtttgttttc ggcgtgcaac agcgcgcgga agaaatgggc
     7441 attctgctgg cgaccggctg gaaaccgttc gccgtccgga accttttctt tatggaaggc
     7501 ggagcgattg cgctcaccgg ttcggcggcc ggcgcgtggc tgggaaccgg ttatacgaaa
     7561 ctgctgattc tcggactcgc cacctattgg cagggtgccg tggcgaattc ggcgattcag
     7621 tattttgccc ggccgggaac ggtggtcgcc ggcgcggcgg tcagctttat ctgcgcgatt
     7681 ctctcaatgg gaatcgccat gtggcggcag accggacatc cggcgcgtga actgctgacg
     7741 gcggattttt cgcagccgcc tttgccgcgg ctgcggcgcg ccaggggagc aggagcattt
     7801 gctgctctcg cgtgcgcgct gggagcagtc gggatggtcg tttatgcgct gaccgcggat
     7861 gtgcagagtg tcacgatgcc gtttttcgga gcgggcgctc tgctgctgat tgcaggcgtt
     7921 ctgttctgcg ggattgcgct gcgcagagcg ggacggcggg gaaatctttc cggtattgcc
     7981 ggaatggcgt tgcgcaatgc ggcgcggcgg cgcggccgca gtctgacagt gatcggcctg
     8041 ctggcctgcg gatgttttct ggtctttacg gtttcgtcga tgaaagaaga tgtgaccgcc
     8101 catgcggacc agccgtggtc gggtaccggc ggattccgct ggttcggcga atccactctg
     8161 ccggtcaccg ataatccggg cggagtccgc ctgcgcgttc gcgacggcga cgatgccggc
     8221 tgtctcaatc tgaaccgcgc gcggtcgccg cgcctgctgg gggttgatcc ggatgtgatg
     8281 tcgcggcgca aagcgtttct ggccggtgac gatgtgtggc agctgctgaa actggaactg
     8341 ccggacggcg ctgttcctgc gctcgccggc gatgcggaca ctgcgatgtg gggactggag
     8401 gcaaagaccg gcgtggagca gggcgcgctg ctggattatc gcgatgaagc cggaaatgtc
     8461 ttcaaggtta aactggtggg ccgtctgccg atgcggctgt cggttttcca gggaactctt
     8521 ctgatggccg aacagcggtt tgttgaaaaa tttcccggcg aagcaggcta ccggatgttt
     8581 ctgtttgacg atcaggccgg cggaacggcg gcggcgaaga aatatgcgcg ggacggaatg
     8641 gatgtcgtac cggccacgca gcggctgatg gaattctacg cggtggaatc gacctatctt
     8701 gcgatgttcc tgctgctggg cgcgctggga ctggcggtcg gcagtatggg catgggcgtg
     8761 gcggtgctgc gcaacgtgca ggaccggcgc actgaaatcg cgctgctccg cgcggtcggc
     8821 taccgcagcc gcacactgcg gaaaatgctt ttcatcgaac acggcctgct gttcgcggcg
     8881 ggacttggtg tcggcgtgat cgcttccgcc gtggcgatgg taccggcgct ggtgatttca
     8941 acatcacagg tttcggcgct cttcctgttc ggcctgctgg ccgctgtcgg aatctgcgga
     9001 gcagtctgca tgattgcggc agtcagcgtc tccttgcgcg gcgatgcgct gcgcggcctg
     9061 cgcaatgaat agcgcgggat agcaaagttc acaacctccc tgcgggaagg tcgtcctaca
     9121 agtttgattg cagggcgggc ttctccggta ttccggagag cctgcgatgt taatctgtcg
     9181 tgcggttttc attacggaat cgtaatcaac aatcggatgg tctgccgccc gccggaacgc
     9241 tatgctcgca tcatgaaagt tctgctggtc gaagatgaag ccaagattgc cgggttcgtc
     9301 cgcaaaggac ttgaggcgca gggattttca gtgacggttt ccggcgacgg cagtgaagca
     9361 tttgaactgg cggtcgccga ttcgttcgac gtcatccttc tcgatatcat gcttccgggc
     9421 cgcgacggtc tgagcattct gaaagaactg cgcgaacgcc gcaataccgt gccggttatt
     9481 ctgctcaccg cgcgcgccga aatgaatgaa cgcgtcgaag gactgaacct cggcgctgac
     9541 gactacatca ccaaaccgtt tttcatggat gaactgacgg cccgcatcaa agcggtcgtg
     9601 cgccgcacct ccggcgaaac gctcagcgta ctgcactccg gcccgctgac gctcaatctg
     9661 attacacggc acgcgcaata cggcgacgaa gaaatcatct tcgcgccgcg cgagttcagc
     9721 ctgctcgaac atctgatgcg ctcgccggga atggttttca cccgcgacca gctgctcgaa
     9781 caggtctggg gctacggctt tgacccgcag accaatctgg tcgaagtctg catccggcgc
     9841 atccgcgaaa agatcgaccg cgacggcaac cagtttattg aaaccgtccg cggcgtcggc
     9901 taccg
//
LOCUS       DAOPZO010000070         9876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_8036_length_9876_cov_8.130638, whole genome shotgun sequence.
ACCESSION   DAOPZO010000070 DAOPZO010000000
VERSION     DAOPZO010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 9876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9876
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8036_length_9876_cov_8.130638"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1286
                     /locus_tag="PKI68_05825"
     CDS             <1..>1286
                     /locus_tag="PKI68_05825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX53243.1"
                     /translation="AAFEELTGLKAENIVGRTVKQVLPDTESRWIEIYGKVALTGVPV
                     RLKDFSRALGRSYSVSAYSPVKGQFATIFSDVTDQKNAEAAVSRARTAAEESNRAKTQ
                     FMANMSHELRTPLNAIIGLTELLEDSPLDDEQRDYVQTIGVSGESLLMLISDLLDFSK
                     IEMGRIDVRKEKFNVREVVAKSMALLSSFAEAKKLTLTESVDADVPETVTGDAVRLQQ
                     VLVNLLNNAIKFTENGFVRLVVRGRVTPAGSRYVEFSVEDSGPGMAAETAKRIFEPFY
                     QADASVTREHGGTGLGLSISKSLVEMMGGEIHVESRAPGGSVFRFYILDHTASGCLVD
                     EAGIRAAWQGRHICVWDNNPADLRAVEYLMERCGIIPVYAESPDKFVESLACNPAPDA
                     VLCNLDMEEFPGRFSELHGMCRDIPWIGVSNWHEPA"
     assembly_gap    1287..1386
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1387..3101
                     /locus_tag="PKI68_05830"
     CDS             <1387..3101
                     /locus_tag="PKI68_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX53244.1"
                     /translation="LEYFTGTDLTFEDVLVPFAGHLNGIPVGRMSPLAGLGFFLSGLA
                     VILLAWRSRDNLRFAALSRASGWLGGLIFLIGFTASLAYFYGHPFLYGRGITVPIALT
                     SALGFILVSLALFGAAGKTVFPLDILTGTSTRCYLLRFILPLSVIAVIAGGLTVLFAE
                     KIAGVNPALLSALLIVVMTLFVGSLASWISRFMGGEIDRFKAAAEQSLKQQEELNAEL
                     NRLAKFPGQNPNPVLRLSEDGTVLYANESSRTLLLNLKQLDGKILQDNFQISFAELLA
                     SGNVYRQEVDCGEIAYALALTPLAGEGMINIYGMDITSRKRAEQQLAATMDQLRVSNR
                     DLEQFAYVASHDLQEPLRMIANYVQLIERRYKDKLDQDARDFIHYAVDGAVRMQQLID
                     SLLEYSRLQSRKKPFNDVDLDGVLKQALHDMERLIVETGARITSDPLPAVRGDEVQLG
                     QIFQNLVSNALKFKDTGRPEIHITAEEIPGYWKISVRDNGIGIEPEHQDKIFKIFKRL
                     HSRADYPGTGIGLAVCKRIIERHGGEIGVESEAGKGSLFWFTLPKKEKDHHGRIQSNR
                     DPAG"
     gene            3064..3501
                     /locus_tag="PKI68_05835"
     CDS             3064..3501
                     /locus_tag="PKI68_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX53245.1"
                     /translation="MAVYRAIEILLVEDNPGDVRLTQEALKENKIRNNLHVAKDGIEA
                     MQFLRRMNGYENTPRPDLILLDLNLPKKDGREVLAEIKTDEKLRAIPVVILTTSDAED
                     DVAKSYDMHANCYVRKPIDLNRFIEVVKIIENFWLSIVELPRS"
     gene            complement(3505..3828)
                     /gene="cutA"
                     /locus_tag="PKI68_05840"
     CDS             complement(3505..3828)
                     /gene="cutA"
                     /locus_tag="PKI68_05840"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNX53246.1"
                     /translation="MSTKLIYVTAPSRKEAERLAETVVTERLAACANILDGVTSIFHW
                     EGNLCRENEAVLFLKTEEDRTEALIARIRQLHSYECPCITVLPVEGGSPDFLQWVKEE
                     TAPNR"
     gene            complement(3825..5318)
                     /gene="zwf"
                     /locus_tag="PKI68_05845"
     CDS             complement(3825..5318)
                     /gene="zwf"
                     /locus_tag="PKI68_05845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNX53247.1"
                     /translation="MSTLEVQIEEPVTFVISGALGDLARRKLIPALYTLFKEGALTAD
                     FAIVGFARRDYDNRSFCKLMEESLREHSRTAVDEPSLKAFCSHLSYYRGDLSDPAAYA
                     GLKAKFDDGTLPANRLFYLSITPDLVESAVTGLRQAGLITPPHSKPWTRTVVEKPFGR
                     NLQSAKALNRMLLDHLDESQIYRIDHYLGKETVQNILSFRFANTIFEPVFNRTYVDNI
                     QITAAETVGMESGRGAFYDQTGALRDMVVNHLFQLLCLVTMEPPDGLEARSIRNEKMK
                     VLRALHLATATTHHPAVCFGQYGGDSSVKAFRQEERVSPQSKTETFAALRMEINNWRW
                     AGVPIYLRTGKRLAKKTTEIAVQFKTPPLSLFQHVACKGDVCDLAGVKPNTLIFRIQP
                     DEGIFLQVSAKRPSMQMVVESVNMDFSYSGKWDKALPEAYERLLLDALRGDSTLFTRS
                     DEVEAEWEVIDDILAIATKSEPAVYAPGSWGPAEADAVLLSTGWKNE"
     gene            5430..6512
                     /locus_tag="PKI68_05850"
     CDS             5430..6512
                     /locus_tag="PKI68_05850"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNX53248.1"
                     /translation="MKILTKYLLKSLISPLLYCLLGFTLIFIISDLFDNFSEFMESGS
                     SPLEILYYYSQILPSVTVLILPACLLLAMLHSLSRLTRHSEITAMRAGGVSIYRIVIP
                     FIGVGIFASLLTAFINEKISPDSSWRAQKFIEYQRAGRDNEIFFARTLALKNRDHVWM
                     VQKLDTRDYSMYNVELVQQRPDGSDAVKYHAEKALWLDGRWWFMDLIIQAYKENGDLN
                     GPPEMVLQKEMRDLRETPQTFMAEIKDPQYLSSQQMRHYLRSKKDISSNTRARLLVDY
                     YSRLASPFTCLIVTLIGVPVGAHTGRRGAFAGIMVAMCLFFGFYILQLTGQALGKQEH
                     IAAWIGGWLPILIFGAASPLFIHRMR"
     gene            6514..7323
                     /locus_tag="PKI68_05855"
     CDS             6514..7323
                     /locus_tag="PKI68_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX53249.1"
                     /translation="MTAHTDNPLVDVQNLQVHFGAVSSPVRAVDGVDFRIFPGEIVAL
                     VGESGSGKSISALSLANLVPEPAARIVGGRIIFQGSDTLTLKNSELRKLRGAKIAYIF
                     QEPATSLNPVYTVGWQIGEAIRLHRKGVNVKAEVEKLLTSVGLPDPARAAEAYPHELS
                     GGMQQRAMIAMALACDPDLLVADEPTTALDVTVQKQILDLLMELREKRGLSILLITHN
                     LGIVANTADRVYVMNSGKIVESGETGTVLRTPQHEYTQKLLKAVPRLHKVS"
     gene            7320..8093
                     /locus_tag="PKI68_05860"
     CDS             7320..8093
                     /locus_tag="PKI68_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX53250.1"
                     /translation="MSKLLQVENVHVRYGGFEAVRGVSLSLAAGETLGLVGESGCGKS
                     SLGRAVLALEPVSAGRVAFEERSVHALKGAALKNFRCQAQMVFQDPFGSLNPRMSVGT
                     AIEEVLYVHGRGANRAERRRKVEALFTDVGLNPDWLSRYPHEFSGGQRQRIGIARALA
                     LEPKLLVADEPVSALDVSVQADIIQLLKRIQRERGLAYLFIGHDLAVVRDMSDRIAVM
                     FKGEVVETGTSGQVCDDPQHPYTQKLLSAVPDIDKALAQ"
     assembly_gap    8157..8256
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8257..8445
                     /locus_tag="PKI68_05865"
     CDS             <8257..8445
                     /locus_tag="PKI68_05865"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HNX53251.1"
                     /translation="RQAIRRGIELMSGNYWRLGLLWLRFIGWQLLAVLTLGVGFIWLA
                     PYYMASMTAFYDDLARNR"
     gene            8620..8883
                     /gene="yajC"
                     /locus_tag="PKI68_05870"
     CDS             8620..8883
                     /gene="yajC"
                     /locus_tag="PKI68_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNX53252.1"
                     /translation="MAFMVLIFYVVMIRPQRRREKERRTLIAAVKSGDRVLLTSGIIG
                     EIAGVKDKTLIVLIAEKTKIEVLKSAISQVLDEKGELPSEARV"
     gene            8903..>9876
                     /locus_tag="PKI68_05875"
     CDS             8903..>9876
                     /locus_tag="PKI68_05875"
                     /inference="COORDINATES: protein motif:HMM:NF019187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53253.1"
                     /translation="MNKSTMWKWLLLIALTAWSMALVTPFKQKVKLGLDLKGGTSFTV
                     EVDKNEVRKQILEGDAKLEGSALESAVQTLVKKTQGVALEVIRSRVDGLGIAEPEIYP
                     LLDNRIVIRLPGVDVNKRIEAKNSIMSVAFLEFRLVHSESDAWVSELFNSGLTPRGFS
                     AVRVGNEQYYVRDRKVVEDKALDRAFWEDLKRFGSKRGAEFMLEKETLKDGSVVYKPA
                     YIEVRKQLTGSALKDARVEYDQYNRPYISLSFNKAGAERFGKVTAAYAPRGENNLNSE
                     TGRRLAIILDGKLYSAPTIQDAIYSGNAQITGSFTVDECTRLVNVLRAG"
BASE COUNT         2101 a         2628 c         2733 g         2214 t
ORIGIN      
        1 tgcggccttt gaggaactca cagggttgaa agctgaaaat attgtcgggc gcacggtcaa
       61 acaggttttg ccggacacag agtcccgctg gatcgaaatc tacggaaagg ttgcgctgac
      121 aggtgttccg gtgagactga aggatttttc gagggcactc ggccggagct attcggtttc
      181 agcctattcg cccgtcaaag gacagtttgc gacaattttt tccgatgtta ccgaccagaa
      241 aaacgctgaa gcggctgtca gccgtgcccg caccgccgcc gaagagtcca atcgggccaa
      301 aacgcagttc atggccaaca tgagtcacga actgcgtacg ccgcttaatg caattatcgg
      361 tctcacagaa ctgcttgaag attctccgct cgacgacgag cagcgcgatt atgtccagac
      421 aatcggtgtc agcggtgaat ccctgctgat gcttatttcc gatctgctgg atttttcaaa
      481 aattgaaatg gggcggattg atgtccggaa agagaagttc aacgtccggg aagtcgttgc
      541 aaaatccatg gcgctgcttt cttcgtttgc ggaggcaaaa aagctgactc tgacggagtc
      601 tgttgatgct gacgttccgg aaacggtcac cggagatgcc gttcgcttgc agcaggttct
      661 ggtgaatctg ctgaataatg cgattaagtt taccgaaaac gggttcgtcc gccttgtggt
      721 gcgcggacgt gtgacgccgg ccggcagccg gtatgttgaa ttttccgtgg aagacagcgg
      781 gccgggaatg gcggcagaaa cagcgaaacg gattttcgag ccgttttatc aggccgatgc
      841 ttctgtaacc cgcgaacacg gcggaaccgg actaggactg tcgatttcaa aaagtttagt
      901 cgaaatgatg ggcggcgaaa ttcatgtcga aagccgggcg cccggggggt cggtattccg
      961 tttttacatt ctggatcaca cggcttcggg ctgtctggtt gatgaagccg gtatacgggc
     1021 tgcgtggcag ggccggcata tctgcgtgtg ggataataac ccggccgatc ttcgtgcagt
     1081 tgagtatctg atggaacgtt gcgggatcat acccgtctat gcagaaagtc cggataaatt
     1141 tgtagagagt ctggcctgta atccggctcc ggatgccgtt ttatgcaatc tggatatgga
     1201 agagtttccc ggacggtttt ctgaactgca cgggatgtgc cgtgatattc catggatcgg
     1261 cgtgtcgaac tggcacgagc cggccgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnctct ggaatatttt acaggaaccg accttacgtt tgaggatgtt ctggttcctt
     1441 ttgccggtca tctgaacggg attcctgtcg gacgaatgtc gccgctcgca gggttgggtt
     1501 tttttctgtc gggactggcg gtcatcctgc tcgcatggcg gagccgggac aatctgcgct
     1561 ttgccgctct ctctcgcgcg tccggctggc tgggcgggct gatatttctg atcgggttta
     1621 cagccagtct ggcgtatttc tacggacatc cgtttctgta cggccgcggc attacggttc
     1681 cgatcgcttt gacctcggct ctggggttta tattggtgag cctcgctctg ttcggagccg
     1741 ccgggaagac agtatttcca ttggatattc tgacgggtac ttccacccga tgttatctgc
     1801 ttcggtttat tcttccgctt tccgttatcg cggtcattgc cggcggatta acggtgcttt
     1861 ttgcggaaaa aatcgccggc gtaaatccgg cgctcctgtc ggcgctgctg attgttgtca
     1921 tgaccctctt cgtcggcagt ctggcctcgt ggatatcccg tttcatggga ggcgaaattg
     1981 accggtttaa agcggccgca gagcagtcgc ttaaacagca ggaagagctt aatgcggaat
     2041 tgaaccggct cgctaaattt cccggccaga atcccaatcc ggttctgcgc ctttctgagg
     2101 acggcacagt actttatgcc aacgaatcca gccggactct cctgctgaac ttgaagcagc
     2161 ttgacggaaa gattttgcag gacaattttc aaatctcctt tgctgaactg ctggcgtccg
     2221 ggaacgtata ccggcaggaa gtggattgcg gcgaaattgc ctatgcgctg gcgcttaccc
     2281 cgcttgcggg cgaagggatg atcaatattt acggcatgga tattacatca cgtaaaagag
     2341 cggaacagca gctggccgca acgatggatc agctgcgtgt ctccaaccgc gatctggaac
     2401 agtttgccta cgtcgcttct cacgacctgc aggaaccgct gcgcatgatt gccaactatg
     2461 ttcagctcat cgagcggcgg tacaaagaca agctcgatca ggacgcccgc gattttatcc
     2521 attacgcggt cgacggcgct gtgcgtatgc agcagctgat cgactcgctg ctggaatatt
     2581 cccggcttca atcccgcaaa aaaccgttta atgacgttga tctggacgga gttttgaagc
     2641 aggcactgca tgatatggaa cggctcatcg ttgagaccgg agcccggatc acgtctgatc
     2701 ctctgccggc agtccggggc gatgaggtgc agttgggtca gatattccag aatctcgtca
     2761 gtaacgcgct caaattcaag gacacaggcc ggcctgagat tcatatcacg gccgaagaaa
     2821 ttcccggata ttggaaaatt tctgtccgcg acaacggaat cggcattgag ccggaacatc
     2881 aggacaaaat ttttaaaatt ttcaaacggc tgcacagccg tgcggactac cccggaaccg
     2941 gaatcggatt ggcggtttgc aaacgaatta ttgagagaca tgggggtgaa atcggtgtag
     3001 agtctgaagc cggaaaggga agcttgttct ggtttaccct tccgaaaaag gagaaagatc
     3061 atcatggccg tatacagagc aatcgagatc ctgctggttg aagataatcc cggcgatgtc
     3121 cgtcttacac aggaggcgct caaggaaaat aaaatccgca acaacctgca tgtggccaaa
     3181 gacggtattg aagcaatgca gtttctccgt cgcatgaacg gttatgagaa cacgccgcgt
     3241 cccgacctga ttcttctgga cctcaatctg ccgaaaaaag atggccgcga agtgctcgcc
     3301 gagattaaaa ccgatgaaaa actgcgcgcc attcccgtag tgattcttac cacctctgat
     3361 gccgaagatg atgtggcgaa atcatacgac atgcacgcca actgctacgt ccgcaaaccg
     3421 attgacctga accgcttcat cgaagtggtg aaaatcatcg aaaatttctg gctgtccatt
     3481 gtcgaattgc cgagaagctg agtttcaccg gttcggcgca gtttcttctt ttacccattg
     3541 caggaagtcc gggctgccgc cttcaaccgg cagaacggta atacatgggc actcgtaaga
     3601 atgaagttgg cggattcgcg caatcagggc ttccgttctg tcttcttccg tcttcagaaa
     3661 cagtaccgcc tcattttcac ggcagagatt tccttcccag tgaaagatcg acgtgacgcc
     3721 atccagaatg ttggcgcagg ccgccagacg ttccgttacg acggtttcag caaggcgttc
     3781 cgcttctttg cgcgacggtg cagtgacata aatcagtttt gtactcattc atttttccag
     3841 ccggtggaaa gaaggacggc atccgcttct gccggacccc acgagccggg cgcataaacg
     3901 gccggttcgc tctttgttgc gatggcgaga atgtcatcaa tcacttccca ttcggcttcg
     3961 acttcgtccg atcgggtgaa aagagtggaa tcgccgcgca aagcgtcgag cagcagccgt
     4021 tcgtaggcct ccggcagggc cttgtcccat ttgccggagt aggaaaagtc catgttcacg
     4081 ctttcgacca ccatctgcat gctggggcgt ttggctgaga cctgaaggaa gatgccttcg
     4141 tccggctgga tacggaaaat cagagtgttg ggctttacgc cggccagatc gcatacgtca
     4201 cctttgcagg cgacatgctg gaagagggag agcggcggcg tcttgaactg caccgcgatt
     4261 tccgtcgtct tttttgccag ccgcttgccg gtgcgcagat aaatcggcac acctgcccag
     4321 cgccagttat taatttccat ccgcagcgcc gcgaaggttt cggtttttga ctgcgggctt
     4381 acgcgttctt cctgccggaa ggctttaaca ctgctgtcgc cgccatactg tccgaagcag
     4441 accgccggat ggtgcgttgt ggcggttgcc agatgcagtg cgcgcagaac cttcatcttt
     4501 tcgttgcgga tcgaacgcgc ttccagcccg tccggcggtt ccatcgtcac cagacagagc
     4561 agctgaaaga gatggttgac gaccatatcg cgcagggcgc ccgtctggtc gtaaaacgct
     4621 ccgcgcccgc tttccatacc gacggtttcg gctgcggtga tctgtatatt atctacatac
     4681 gttcggttga aaaccggctc gaaaatggtg ttggcgaagc ggaacgaaag aatgttctgc
     4741 accgtctctt tgccgaggta gtggtcaatg cggtaaattt gcgactcatc cagatggtcg
     4801 agcagcatcc ggttcagcgc cttggcgctt tgcagatttc gtccgaacgg tttctcgacc
     4861 actgtccggg tccagggttt ggagtgcggc ggagtaatca gtccggcctg ccggagtccg
     4921 gttacggcgg actcaaccag atccggtgtg atcgagagat aaaaaagccg gtttgcgggc
     4981 agggtgccgt cgtcgaactt tgctttcagt ccggcgtatg ccgccgggtc ggacaggtcg
     5041 ccgcggtaat agctgagatg tgagcagaaa gccttcagcg agggttcgtc aacagctgta
     5101 cggctgtgct cgcgcagaga ctcttccatc agcttgcaga aactccggtt gtcgtaatcg
     5161 cggcgcgcga aaccgacgat ggcaaaatcg gccgtcagcg caccctcttt aaaaagagtg
     5221 tagagcgcgg ggatcagttt gcggcgggcc agatcgccta atgcgccgga aattacaaat
     5281 gttaccggtt cttcgatttg gacttccaat gtagacatgg cccctccgtt caggttgcgc
     5341 gcagtatccg gtattcgaac ctcttcttca agtcgcatac atcggcactt gcgagcctgc
     5401 ggctttctcg gtaccgtctg aatccttaca tgaaaatcct cactaaatat ctgctgaaaa
     5461 gtctgatttc tccgctgctc tactgtctgc tcggatttac tctgattttc atcatcagcg
     5521 atctgttcga taacttcagc gaatttatgg aaagcggcag cagtccgctc gaaatactct
     5581 attattacag ccagatcctg ccgtcggtta ccgttctcat tctgccggcc tgcctgctgc
     5641 tggccatgct ccacagcctt tcacgcctca cccggcatag cgagatcacg gccatgcgcg
     5701 ccggcggggt cagcatctac cgcatcgtta tccccttcat cggcgtcggc atcttcgcca
     5761 gcctgctgac agctttcatt aatgaaaaga tttctccgga ctcttcctgg cgcgcccaga
     5821 aattcatcga ataccagcgg gccggccgcg ataatgaaat tttcttcgcc cgcacactgg
     5881 cgctcaaaaa ccgcgaccat gtctggatgg tgcagaagct cgatacgcgc gactactcaa
     5941 tgtacaacgt cgaactcgtt cagcagcggc ccgacggatc ggacgccgtc aaataccatg
     6001 ccgaaaaagc gctctggctg gacggccgct ggtggttcat ggacctcatc attcaggcct
     6061 acaaagaaaa cggcgacctg aacgggccgc cggaaatggt tctccagaaa gaaatgcgcg
     6121 acctgcgcga aacaccgcag acctttatgg cggaaattaa agacccgcag tatctctctt
     6181 cgcagcagat gcgccattat ctccgctcta aaaaagacat ttccagtaac acccgcgccc
     6241 gcctgctggt ggactactac agccgcctgg cttcgccctt tacctgtctc atcgtcaccc
     6301 tcatcggcgt gccggtcggt gcgcacaccg gacggcgggg agcgtttgcc ggcatcatgg
     6361 tcgccatgtg tctcttcttc ggtttctaca tcctgcagct cacgggtcag gcgcttggca
     6421 aacaggaaca cattgccgca tggatcggcg gctggctgcc tattctgatc ttcggtgcgg
     6481 cgagcccgct attcattcac cggatgcgtt gaaatgaccg cacataccga caacccgctt
     6541 gtggatgtac agaaccttca agtgcacttc ggcgctgtct ccagccccgt ccgcgcagtg
     6601 gacggggtgg atttccggat tttccccggc gaaatcgtcg ccttggtcgg cgaaagcggc
     6661 agcggtaaaa gcatcagcgc tctttcgctg gccaatctgg tgccggaacc tgccgcgcgg
     6721 attgtcggcg gccggatcat tttccaggga tcggacacgc tgacgctcaa aaattccgaa
     6781 ctccggaaac ttcgcggcgc gaaaatcgcc tacattttcc aggaaccggc gacttcgctg
     6841 aatcccgttt ataccgtcgg ctggcagatc ggcgaagcca tccgcctgca ccgcaaaggc
     6901 gtcaacgtaa aagccgaggt cgaaaagctg cttacctcgg ttggactgcc ggatccggcg
     6961 cgcgcagccg aagcctaccc gcacgaactt tccggcggta tgcagcagcg cgccatgatc
     7021 gccatggcgc ttgcctgcga tcccgatctg ctggtcgccg acgagccgac caccgcgctc
     7081 gacgtcaccg tccagaaaca gattctcgat ctgctcatgg agctgcgcga aaagcgcgga
     7141 ctttccattc tgctgatcac ccacaacctc ggcatcgtcg cgaacacggc ggaccgcgtg
     7201 tatgtgatga acagcgggaa aatcgtcgag tccggtgaaa ccggaaccgt tctgcgcact
     7261 ccgcagcacg aatacactca gaaactgctc aaagcggttc caaggcttca taaagtgtca
     7321 tgagtaagct gctgcaagtc gagaatgtac acgtccggta cggcgggttc gaagccgtgc
     7381 gcggcgtcag cctttcgctg gcggcgggcg aaacgctcgg tctggtcggc gaaagcggct
     7441 gcggcaaaag ctcgctcggc cgcgccgttc tcgcgctgga gccggtttcg gcgggcaggg
     7501 tggcgtttga ggaacgcagc gttcatgctc tcaaaggagc ggcgctcaag aatttccgct
     7561 gtcaggcgca gatggttttt caggatccgt tcggatcgct gaacccgcgg atgagtgtcg
     7621 gcacggcgat tgaagaagtt ctttatgtcc acggccgcgg cgcaaaccgc gccgaacgcc
     7681 gccggaaagt cgaagcgttg tttacagatg tcgggcttaa tccggactgg ctgagccgct
     7741 atccgcacga attcagcggc ggacagcgcc agcgcatcgg cattgcccgc gctttggcgc
     7801 tggagccgaa actgctggtc gccgacgaac cggtttcggc gctcgatgtg tctgttcagg
     7861 cggacattat ccagcttcta aaacggattc agcgcgaacg cgggctggcc tatcttttta
     7921 tcggccacga cctggcggtg gtgcgcgata tgagcgaccg catcgccgtc atgtttaaag
     7981 gcgaagtcgt cgaaaccggc acctccggtc aggtttgcga cgatccgcag catccctaca
     8041 cgcagaaact gctttccgct gtgccggata ttgataaagc cttggctcaa taaggaggaa
     8101 tccatgaaaa gtatgacgcc tattcgtgac ctgaccgccg ctgcccgtgc cggactnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncggc aggcgatccg gcgaggcatt
     8281 gaactgatga gcggtaatta ttggcggctg ggactcctct ggctccggtt tatcggctgg
     8341 caactgctcg ctgtcctgac acttggggtc ggttttatct ggctggcacc ctattacatg
     8401 gcgtcgatga ccgcttttta cgatgatttg gcaaggaata ggtaaacagc gggattcaag
     8461 cttgccaagt acgggggcgg actctagcat gtccgccttc gtgttttttg tggcaaacta
     8521 agggagaaaa gtatgttttt acagacaatc gctatggctc cgcctccggg cgcaaacggt
     8581 caacctgggc aggcctcacc gttgcagttt tacggaatga tggcttttat ggttcttatt
     8641 ttttatgtcg tcatgatccg cccgcagcgc cgtcgcgaaa aagaacgcag aaccctgatc
     8701 gccgccgtta agagcggtga ccgcgtactg ctcaccagcg gcattatcgg cgaaattgcc
     8761 ggcgtgaagg ataaaaccct gatcgtgctg atcgctgaaa aaacgaaaat tgaagtgctg
     8821 aagagcgcca tttcgcaggt tctggatgaa aaaggcgaac ttccctctga ggcaagagtt
     8881 taacccgccg gggatttgat ttatgaacaa aagcacaatg tggaagtggc tgttgctgat
     8941 cgcactgacg gcgtggtcga tggctctggt tactccgttt aagcaaaagg taaaactcgg
     9001 actcgacctg aagggcggaa cgagcttcac cgtggaagtg gacaagaacg aagtccgtaa
     9061 gcagattctg gaaggcgatg ccaagctcga aggttccgcg cttgaatccg ccgtccagac
     9121 tctggttaaa aagacgcaag gcgtggcgct ggaagttatc cgcagccgcg tggacggcct
     9181 tggtattgcc gaaccggaaa tctatccgct gctcgacaac cggatcgtta tccgtctgcc
     9241 cggtgtagat gtgaacaaac gcatcgaagc caaaaactcg atcatgagcg tggcgtttct
     9301 tgagttccgt ctggttcatt ccgaaagcga tgcctgggtg agtgagctgt ttaactcggg
     9361 actgactcca cgcgggttca gcgctgtacg cgtcggtaat gagcagtact atgttcgcga
     9421 ccgcaaggta gtcgaagata aagctctcga ccgcgcattc tgggaagatc ttaagcgttt
     9481 cggatccaaa cgcggcgccg agtttatgct tgaaaaagaa accctgaagg acggttccgt
     9541 cgtttataag ccggcttata tcgaagtccg caaacagctc accggttcgg ctctcaaaga
     9601 tgcccgggtc gagtatgacc agtacaaccg gccttacatt tcactttcct tcaacaaagc
     9661 cggagccgaa cgctttggta aggttacggc cgcctatgcg ccgcgcggag aaaacaatct
     9721 gaacagcgaa accggccgcc ggctggccat cattctcgac ggcaagctct attccgcacc
     9781 gacgattcag gatgcgattt atagcggcaa cgcccagatt accggttcgt tcactgttga
     9841 tgaatgcacc cgcctggtca acgtactgcg cgccgg
//
LOCUS       DAOPZO010000071         9870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_8043_length_9870_cov_8.705043, whole genome shotgun sequence.
ACCESSION   DAOPZO010000071 DAOPZO010000000
VERSION     DAOPZO010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8043_length_9870_cov_8.705043"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PKI68_05880"
     CDS             <1..411
                     /locus_tag="PKI68_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53254.1"
                     /translation="DTIQRTGWKFVQIMKGDAEKGFPTKGKLFDAILLDVPCLNTGVL
                     RRRVDARWRINRERIEMLKKTQWQILTAMSKCVKSGGRIVYSTCSLEVEENEELVSRW
                     AREHKEFRLVKAKRIFPPKSGTDGAYAALLVRAE"
     gene            560..1141
                     /locus_tag="PKI68_05885"
     CDS             560..1141
                     /locus_tag="PKI68_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNX53255.1"
                     /translation="MKRVFLIGPGGAGKTTCGKVFAGLIGYVFVDLDSEFMARVGHIG
                     QHIEDKGYLSYCRSNSALFYALIEEQSEDTVFALSSGFLVHEDTDPELSKHKDAIRDL
                     GCSILLLPSRSIEETEKIIISRQMSRGIACQQKSERRKIRDRFLKYYKHGDIQIFSTE
                     NPTCVAEEMKTKYMEFAEPARGPYAVKRTIFVP"
     gene            complement(1237..1812)
                     /locus_tag="PKI68_05890"
     CDS             complement(1237..1812)
                     /locus_tag="PKI68_05890"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNX53256.1"
                     /translation="MKVVAFNGSPRKGGNTEFLLKTALKELEAAGIETELIQVGGQML
                     RGCTACGWCRSSGEKRCVIDTDPINDYINKMREADGILLGSPTYFADLTAEMKALIDR
                     AGYVLRPGMELRYKVGAGLVAARRGGAIHTFDSLNHFFLLNEMLVVGSCYWNDGYGGP
                     VGAVEANDAEGAKTAKILGQNMVWALKKLCG"
     gene            complement(1825..2166)
                     /locus_tag="PKI68_05895"
     CDS             complement(1825..2166)
                     /locus_tag="PKI68_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53257.1"
                     /translation="MNVKLRRLTLLLLCASGTGFSQTVHYGVGVTDMYCPYCLYNHYG
                     FPCEHRYTVRVLDYPLVEKPKELEPVKPHPVIDPIRARLEKRMIEIDSAGFANTNKTR
                     IEIVPGLTPKK"
     gene            complement(2179..3087)
                     /locus_tag="PKI68_05900"
     CDS             complement(2179..3087)
                     /locus_tag="PKI68_05900"
                     /inference="COORDINATES: protein motif:HMM:NF010612.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HNX53258.1"
                     /translation="MDIFSLIFIVVGLCLFETITSIDNAIINAEVLSTMQAKARRWFL
                     VWGLFIAVFLVRGLLPWIIIWAAMPTLGPIGAFTATFSGDAAVIEAIEKAAPMLLIGG
                     GTFLLFLFFHWLFLEPKNYGLSGEAFFQKHGVWFFAIVAILLAVLVWFSLKTNPMMAF
                     GSVVGSTAFFITHGFRQQAEHAEKNLMQNGLSDWSKILYLEVIDATFSIDGVLGAFAF
                     TLSVPLILLGNGLGALVVRQLTVGNVDRIKKYLYLKNGAMYSVLFLGIFMTLESFGFH
                     LPVWLSPLMTCGVVSFFFIKSKAALR"
     gene            3239..4126
                     /locus_tag="PKI68_05905"
     CDS             3239..4126
                     /locus_tag="PKI68_05905"
                     /inference="COORDINATES: protein motif:HMM:NF017069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNX53259.1"
                     /translation="MDVNLRNEVIRWQEKLFKRSVRRQMRLRELRELIGITTNQNCLE
                     ITAGDGMISTRLRDGGGRWKTLVLSNDVKTALDWFVPDQVEVLQDSIKEADGTFDLVV
                     IVDALERVRDDYAFIKECHRVLKSDGRLVVTAARKMLCLGTCPLRSMLGLSWRAKGLE
                     RPGYTSGEFFEVLKDGFDVPETDSYSTCCVELPGLLCEAAANKLLGGLYNMPGENTGT
                     EEFYHYTKLNIFATLVYPLMWVLARLEEKLFFFAPGHNMVAKTKRRVWRARKQPVLID
                     GRSIAEAAINTKIGTAAPF"
     gene            4203..4346
                     /gene="rpmH"
                     /locus_tag="PKI68_05910"
     CDS             4203..4346
                     /gene="rpmH"
                     /locus_tag="PKI68_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNX53260.1"
                     /translation="MKRTWHPSKIKRARKHGFRARMETADGRKVLARRRRKGRKRLTV
                     CDA"
     gene            4372..4728
                     /gene="rnpA"
                     /locus_tag="PKI68_05915"
     CDS             4372..4728
                     /gene="rnpA"
                     /locus_tag="PKI68_05915"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNX53261.1"
                     /translation="MEPKTLSKKQRLIQASLFGEAFAQKRNFVGRYMVFWLREGADAA
                     LRLGVVTSKKVSNRAVDRNLARRYLREIFRQHREELSGPVDLVIIARRNLLDAPYADV
                     EQEFLRLAEKAGLLKR"
     gene            4725..4952
                     /gene="yidD"
                     /locus_tag="PKI68_05920"
     CDS             4725..4952
                     /gene="yidD"
                     /locus_tag="PKI68_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNX53262.1"
                     /translation="MKRLLIALVRIYQKTGSRLKPACCRFYPSCSDYTIEAIQKHGIV
                     YGLWLSFRRICRCHPWNPGGYDPVPDRKQEK"
     gene            4957..6672
                     /gene="yidC"
                     /locus_tag="PKI68_05925"
     CDS             4957..6672
                     /gene="yidC"
                     /locus_tag="PKI68_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNX53263.1"
                     /translation="MKKNDFIIVGILVALMFAWMNFYPAIEKKFFPRPEQPAAQAVPA
                     ADKPVVSAPVEKAAVIAEAAAVKAPEAVLPAVAEQRMTLSNDDIELTVSSYGGAVVSA
                     VLKKYPETNRKDSGPAVLDFSAAPALRYSGLPVSAGILEKTATGLRYTAALGDNRFFE
                     RTLKLEGAYLLKIEDAFVNSSSAPWALPQLRLPTGPMAEAKGTTAMAGVVTLGVDSFA
                     PAEGVHHWGKELNKLFGAAAGNELKTTTAEQIAPRPVDWIAAKNKFFVQILTPEAAAG
                     GFDLSVRRAGEGKKNVPVEVAAALQFNAGLVAGNETVTRKTSAYIGPKDYSFLKTQGK
                     EQVEVMEFKSTGFWKFMNPVMYPIKTGLLWGLKALEKVVRNYGVAIMLLTVIVRIIFW
                     PITHKGTESMRKMQALQPQLKEIREKFKDNPQRMQQETMAFYKENKVNPMGGCLPMLI
                     QIPVFIALFVVLRSAVELRFSKFLWIRDLSEPENLFAGMIPVVGSLNILPLLMSATMV
                     LQQKMTPSAGDPQQQKMMAIMMPIMMLFFFYTMPSGLVLYWTTSNVIMIVQMMIKKQA
                     PVAVK"
     gene            6740..7195
                     /locus_tag="PKI68_05930"
     CDS             6740..7195
                     /locus_tag="PKI68_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53264.1"
                     /translation="MKLFRIISFLSMAAVLSGCASSNSGEVYSRNQARRTQTVQPGTV
                     QFVKSVKIEGTRSAVGPVAGGVAGGVAGSTMGAGKGSVLAALGGAAIGAVGGAVAEEQ
                     LTKKAGLEITVKLDSGSTIAVVQEADIMFSVGERVRVLTGSDGTTRVVK"
     gene            complement(7200..9110)
                     /gene="glgX"
                     /locus_tag="PKI68_05935"
     CDS             complement(7200..9110)
                     /gene="glgX"
                     /locus_tag="PKI68_05935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HNX53265.1"
                     /translation="MKPGVYIRKDEIEFAVHSSANRVWLLLFDSTDAGTPAREIEMSG
                     GPLWRVKVPGLRPGAFYLYRTDAVPEQWLLDPYARAVHFPRAWGETAGLEPGAHIRTG
                     KQFPKCVIPDESFDWHGDRHPHTPLEKTVIYEAHLRGFTRGDGGGSYLDFIDKIPYLQ
                     SLGITAVEFLPVFEFNELEFFLEGAARKELLNFWGYSTLAFFAPMSRYAASREPGAAV
                     HEFKTLVRALHAAGMEVILDVVFNHTGEGPFNGPVYSFRGLDEAAYYMIRHDGTYPNW
                     SGCGNTFNCNHPVARQLIVDALVYWAKEMHVDGFRFDLATVLCRGEDGALLEKPPVIE
                     AIDAEPALKKVKLIAEAWDAHGGYQVGSFPGKRFSDWNGRFRDDMRRFWNDSKTAGAL
                     ATRLAGSSDLYEHNGNSPLKSINFITAHDGFTLADLVSYERKYNESNGEQNRDGENHN
                     YSVNFGIEGPLDDPKLCARRLKQQKNLLAMLFLARGVPMLTAGDEFGRTQTGNNNAYC
                     QDNRISWIDWTLLEKNNELFEFTQKLITLRKKHHVLCAGKFYTGDGDVEWIGPESREV
                     DWQHDCALGMHIKGKDALLILINNEEADLSFELPAGRWELVLATDGSTIEPMRGDFAF
                     LVPAHSVCVLSA"
BASE COUNT         2148 a         2634 c         2895 g         2193 t
ORIGIN      
        1 gacaccatcc agcgcaccgg ctggaaattt gtacagatta tgaaaggcga cgccgaaaag
       61 ggttttccga ccaaaggcaa actgttcgac gcgatcctgc tcgacgtgcc gtgcctgaac
      121 accggcgtac tgcgccgccg tgtcgacgcc cgctggcgga tcaaccgcga acgcatcgaa
      181 atgctcaaaa agacccagtg gcagattctc accgccatgt ccaaatgcgt gaaatccggc
      241 ggacggatcg tctacagcac ctgcagcctc gaagtggaag agaacgaaga actggtcagc
      301 cgctgggcgc gcgaacataa agaattccgg ctggtaaaag ccaaacgcat cttcccgccg
      361 aaatccggca ccgacggcgc ttacgccgcc ctgctggtca gagcggaata gtggattgat
      421 ggattggcgg attgttgggt tccaatgttt gggaactttt cttccaaatt tgacgaagcc
      481 gtcacagcag gctacgcttt ccgcaattcg gcgttcggta attccgttgt gacgcccaaa
      541 caacgttctg cataaaaata tgaaacgagt gtttttaata ggcccaggtg gcgctggcaa
      601 gacgacatgt ggcaaagtgt tcgctggcct cattggctac gttttcgtcg atctcgattc
      661 ggagttcatg gcccgagttg ggcatattgg acaacatatt gaggacaaag gataccttag
      721 ttattgccgt agcaactccg cattgttcta cgcgctcatc gaagaacagt cggaggacac
      781 tgtgtttgct ctttcgtcag gctttcttgt acacgaagat acagacccag aactgtcaaa
      841 gcacaaggat gcaattcgtg atcttggatg ttcgattcta cttctgccgt ctcgttcaat
      901 agaggagaca gagaagataa tcatctctcg ccagatgtca cgagggattg cgtgtcagca
      961 gaaatctgaa cggagaaaaa ttcgggacag atttctgaaa tattataaac atggtgatat
     1021 tcagatcttc tctactgaaa atccgacctg tgtggcggag gaaatgaaaa caaaatacat
     1081 ggaatttgca gaaccagcgc gtggacctta cgcggtaaaa cgcacaatct tcgtgcctta
     1141 agtttatgtc ccgcgcaggt cacacgtaac gttcatatgg cgcaggttct aagctttgga
     1201 aaagaccgcc tgactcgttc caaaccttgg aaaaaatcag ccgcagagtt ttttgagggc
     1261 ccagaccatg ttttgtccga gtattttggc ggttttcgca ccttcggcgt cgttggcttc
     1321 gactgcgccg acggggccgc cgtagccgtc gttccagtag caggagccga cgacgagcat
     1381 ttcgttcagc aggaagaagt ggttgagcga gtcgaaggtg tggatggctc cgccgcggcg
     1441 ggccgcgacg agtccggcgc cgactttata ccgcagttcc atccccggac gcaggacata
     1501 accggcccgg tcgatcagcg ctttcatttc agcggtcaga tcggcgaagt aggtcggcga
     1561 gccgagcagg atgccgtcgg cttcgcgcat tttgttgatg tagtcgttga tcgggtcggt
     1621 gtcgatgacg cagcgttttt cgccggagct gcggcaccag ccgcaggcgg tgcagccgcg
     1681 cagcatctgc ccgccgactt gaatgagttc ggtttcgatt cctgccgctt ccagttcttt
     1741 gagcgcggtc ttgagcagga attccgtgtt gccgcctttg cgcggactgc cgttgaatgc
     1801 gacgactttc atgatggact ccttttattt tttcggcgtg aggccgggga cgatttcaat
     1861 ccgggttttg ttggtgttcg cgaaaccggc ggagtcgatt tcaatcatgc gtttttccag
     1921 ccgggcccgg atcgggtcga tgaccggatg cggtttgaca ggttcaagtt ctttgggttt
     1981 ttcgaccagc ggatagtcga gcactcttac agtgtagcgg tgttcgcagg ggaatccgta
     2041 gtggttgtac aggcagtaag ggcagtacat atcggtgacg ccgacgccgt aatgcacggt
     2101 ctgcgaaaat cctgtgccgg aagcgcagag cagcaggagc gtcaaccgcc ggagtttaac
     2161 attcatagtt cgacacggtc agcggagcgc cgctttggat ttgatgaaga agaagctgac
     2221 gacgccgcag gtcatgagcg gcgagagcca gacgggcagg tgaaagccga agctttccag
     2281 cgtcatgaag atgccgagaa aaaggacgga gtacatcgct ccgtttttga ggtagagata
     2341 ttttttgatc cggtcgacgt tgccgacggt gagctggcgg acgaccagcg cgccgagtcc
     2401 gttgccgagc agaatcagcg gcacggagag cgtgaaggcg aacgcgccga gtacgccgtc
     2461 gatggagaag gtggcgtcga tgacttcgag gtagaggatt ttgctccagt ccgagagtcc
     2521 gttctgcatc aggttttttt cggcgtgttc ggcctgctgc cggaagccgt gggtgatgaa
     2581 gaaggcggtg gatccgacga cggaaccgaa ggccatcatc ggattggttt tcagcgagaa
     2641 ccagacaagg acggcgagca gaatggcgac gatagcgaaa aaccagacgc catgtttctg
     2701 aaagaaggct tcgccggaga gtccgtagtt tttgggttcg agaaagagcc agtggaagaa
     2761 caggaacagc agaaaagttc cgccgccgat gaggagcatc ggcgccgctt tttcgatggc
     2821 ttcaatgaca gcggcatcgc cgctgaacgt tgcggtgaac gcgccgatcg gcccgagcgt
     2881 gggcatcgcg gcccagatga taatccacgg aagcagtccg cggacgagga agacggcgat
     2941 aaacagcccc cagacaagaa accagcgccg cgctttggcc tgcatggtgg agagcacttc
     3001 ggcgttaatg atggcgttgt cgatgctggt gatggtttcg aacaggcaga gtccgacgac
     3061 gataaaaatg agggagaaga tatccatatg gtgcagttcc tttttttcgc ggcagagtgt
     3121 ggaaacccgg cccggttctg tcaatttgaa agcggcctca ctctgcgtct tcaggagaga
     3181 atcttttttc tctttgcgcc cgttggggtt atgcctatac tccgccgcca tgaaagcaat
     3241 ggatgtcaat ttgcgtaatg aagtgatccg ctggcaggaa aaactgttta aacggtctgt
     3301 ccggcggcag atgcggttac gtgaacttag agagctgatc gggatcacaa caaatcagaa
     3361 ctgtctggaa atcactgccg gcgacggcat gatcagcacc cggctgcgcg acggcggcgg
     3421 acgctggaaa acgctggtgc tttcaaatga tgtgaaaacc gcgctggact ggtttgtacc
     3481 ggatcaggtg gaggttctgc aggacagcat taaagaggcc gacggaacgt ttgatctggt
     3541 ggtgattgta gacgcgctgg agcgtgtccg tgatgactat gcctttatca aagagtgcca
     3601 ccgtgtgctg aagtccgacg ggcgtctggt ggtaaccgcc gcgcgcaaaa tgctttgtct
     3661 gggaacctgc ccgcttcgtt ccatgctggg gctttcctgg cgcgccaaag gactcgaacg
     3721 tcccggttat accagcggcg agtttttcga agtactcaag gacggctttg atgtgccgga
     3781 aaccgattcc tattccacct gctgcgtcga gcttcccggt ctgctttgcg aggcggcggc
     3841 caataaatta ctcggcgggc tctataatat gcccggtgaa aacaccggca ccgaagagtt
     3901 ctatcactat accaagctga acattttcgc cacgctggtt tatccgctga tgtgggtgct
     3961 ggccagactg gaggaaaaac tgtttttctt tgcgccgggg cataacatgg ttgcaaaaac
     4021 caagcgccgc gtctggcgcg cccgcaaaca gccggtactg attgacggcc ggtcgatcgc
     4081 tgaagccgcc attaacacca aaatcggcac ggccgcgcca ttttagccgg taaaggggtt
     4141 gacagcggcg gacgaacttg gcagggtttg cgctcttttt gaatttcagt ggagattgaa
     4201 caatgaaacg tacatggcat ccttcgaaaa ttaaacgcgc gcgcaaacac ggtttccgtg
     4261 cccgtatgga aacagcagac ggccgcaaag ttcttgcccg ccgccgccgt aaaggccgca
     4321 aacgtttgac cgtttgtgat gcataaagcg ctgtcaccgg aggacttacc ggtggaaccc
     4381 aaaacgcttt ctaaaaaaca gcgtctgatc caagcctccc tcttcgggga ggcttttgca
     4441 caaaaaagga acttcgtcgg acgctatatg gtgttctggc tccgcgaagg cgcagatgcc
     4501 gcgctgcggc tcggcgtggt taccagtaaa aaagtcagta atcgcgcggt cgaccgcaat
     4561 ctggcccgcc gttatttgcg cgaaattttc cgccagcacc gcgaagagct ttccggcccg
     4621 gtcgatctcg tcatcatcgc ccgccgcaat ctgctcgatg ccccgtatgc cgacgtcgaa
     4681 caggaatttc tccggctggc cgaaaaagcg ggactgctga aaagatgaaa cgcctgctta
     4741 tcgctctggt ccggatttac cagaaaaccg gctcgcggtt gaagccggcc tgctgccgct
     4801 tttaccccag ttgctcggat tatacgattg aagcgattca gaagcatggc atagtttacg
     4861 gcctttggct gagtttccgg cgcatctgcc ggtgccaccc gtggaacccc ggcggttacg
     4921 accctgtacc ggaccgcaaa caagagaagt gagattatga agaaaaacga ttttattatt
     4981 gtcggcatcc tggtggcgct gatgttcgcc tggatgaatt tctaccctgc gatcgaaaag
     5041 aaattcttcc cccgtccgga acagccggcc gcacaggccg ttccggcggc ggacaagccg
     5101 gttgtctcag ctccggttga aaaagcggct gtaattgcag aagccgccgc cgtcaaagcg
     5161 ccggaagcgg ttcttcctgc cgttgccgaa cagcggatga cgctgtccaa cgacgatatc
     5221 gaactgaccg tttcctccta cggcggcgcc gtggtttccg ccgttctgaa aaaatatcct
     5281 gaaaccaacc gcaaggacag cggtccggca gttctggact tttccgccgc gccggcactg
     5341 cgttactccg gtctgccggt gtcggccggc attctcgaaa aaaccgccac cggactgcgt
     5401 tataccgcag cgctcggcga caaccgcttt ttcgaacgca cactcaagct tgaaggcgca
     5461 tatctgctca aaattgaaga cgccttcgtc aacagcagca gcgcgccgtg ggcgctgccg
     5521 cagctgcgtc tgccgaccgg cccgatggcg gaagccaaag gaacgactgc gatggcaggc
     5581 gttgtcacac tgggcgttga ttcctttgca ccggccgaag gcgttcatca ttggggcaaa
     5641 gagctgaata aactgttcgg tgccgccgcc ggaaatgagc tgaagaccac cacggcggag
     5701 cagattgcac cgcgtccggt ggactggatt gccgccaaaa ataaattttt cgtgcagatt
     5761 ctgactcctg aagccgctgc cggcgggttt gatctttcag ttcgccgggc cggcgaaggg
     5821 aagaaaaacg taccggtcga agtggctgcc gcgcttcagt ttaatgccgg gctggttgcc
     5881 ggcaacgaaa ccgttacccg taaaaccagc gcctacatcg gcccgaaaga ctattccttc
     5941 ctgaaaacgc agggtaagga gcaggtcgaa gtgatggaat tcaagagtac cggcttctgg
     6001 aaattcatga atccggtcat gtatccgatc aagaccggtc tgctctgggg cctgaaagcg
     6061 ctcgaaaaag tggtacgaaa ctacggtgtc gccatcatgc ttctcaccgt tatcgtccgc
     6121 atcattttct ggccgatcac ccataaaggc actgaaagca tgcggaaaat gcaggcgctt
     6181 cagccgcaac tcaaagaaat ccgcgaaaaa ttcaaagaca atccgcagcg catgcagcag
     6241 gaaaccatgg cgttctataa ggaaaacaag gttaacccga tgggcggctg cctgccgatg
     6301 ctcattcaga ttccggtttt catcgcgctt tttgtcgttc tgcgcagcgc ggttgaactg
     6361 cgcttctcca agttcctttg gatccgcgac ctgtccgagc ctgaaaatct tttcgcgggc
     6421 atgattccgg tggtcggttc gctcaatatt ctgccgctcc tgatgtccgc caccatggtt
     6481 cttcagcaga aaatgacgcc cagcgcgggc gatcctcagc agcagaaaat gatggcgatc
     6541 atgatgccga tcatgatgct ctttttcttc tacaccatgc cgtccggtct ggttctctac
     6601 tggacgacca gcaatgtgat catgatcgtg cagatgatga tcaaaaagca ggcgccggta
     6661 gcggtaaaat aaagccggcg cttccgtttt caaacggcgg aaactgcgct acactgacgc
     6721 ccgttaaaag gagggcgtca tgaagttatt ccgtattatt tcgtttctat caatggctgc
     6781 tgtgctgtcg ggttgcgcat ccagcaactc cggcgaagtt tattcccgca atcaggctcg
     6841 ccgcacccag acggtacagc cgggtacagt tcaatttgtt aaaagtgtga aaatcgaagg
     6901 cacccgttcc gctgtcggcc ctgtcgccgg cggggtggcc gggggcgtgg ccggcagtac
     6961 gatgggggcc ggcaaaggct ctgtgctggc ggcgctgggc ggcgcggcga tcggtgcggt
     7021 cggcggggcc gtggcggaag agcagctcac caaaaaagcc ggtcttgaaa taacggtcaa
     7081 actcgacagc ggttcgacca ttgccgtggt gcaggaagcc gacattatgt tttcggtcgg
     7141 cgaacgggtt cgtgttctga ccggttccga tgggaccacg cgtgttgtga aataatccgc
     7201 tatgcgctga gcacgcagac ggaatgtgcc ggcacaagga aggcaaagtc gccccgcatc
     7261 ggttcgatag tcgagccgtc agtcgccagc accagttccc agcgtccggc cggcagttcg
     7321 aaagaaagat cggcctcttc gttgttgatg agaataagca gagcgtcttt ccccttgatg
     7381 tgcatgccca gcgcgcagtc gtgctgccag tcaacctcac ggctttccgg gccgatccat
     7441 tcgacatctc cgtcgccggt atagaatttt ccggcgcata gaacgtgatg ttttttgcgc
     7501 agggtaatca gtttttgtgt gaattcgaac agttcgttgt ttttctcaag gagcgtccag
     7561 tcgatccagg aaatacggtt gtcctgacaa taggcgttat tgtttcccgt ctgtgtccgg
     7621 ccgaactcat cgccggccgt cagcatcgga actccgcgcg caaggaaaag catcgccagc
     7681 agatttttct gctgcttcag gcggcgggcg cacagcttcg gatcgtccag cgggccttcg
     7741 atgccgaagt tcacgctgta gttgtggttt tcgccgtcgc ggttctgttc cccgttggat
     7801 tcattgtatt tgcgttcata gctcaccaga tcggccagcg tgaagccgtc gtgcgcggtg
     7861 ataaaattaa tgctcttgag cggcgagttt ccgttgtgct catacaggtc ggagctgccc
     7921 gccagccgcg tcgccagcgc cccggcggtt ttgctgtcat tccagaacct ccgcatatcg
     7981 tcgcggaagc gtccgttcca gtccgaaaac cgtttgccgg gaaaactgcc gacctgatag
     8041 ccgccgtgcg cgtcccacgc ctcggcaatc agcttaactt tcttcagcgc gggctcggca
     8101 tcaatcgcct caatcaccgg cggcttttcg agcagggcgc cgtcctcgcc gcggcagaga
     8161 acggtcgcga ggtcgaagcg gaacccgtcc acgtgcatct ccttcgccca gtagaccagc
     8221 gcatcgacaa tcagctgccg ggcgacggga tggttgcagt tgaacgtatt gccgcatccg
     8281 ctccagttcg gataggtgcc gtcgtggcgg atcatgtaat aggccgcctc gtcgagcccg
     8341 cggaaactgt aaaccggccc gttgaacggc ccttcgccgg tgtggttgaa aacgacgtcg
     8401 agaatgactt ccatgccggc ggcatgcaaa gcccgcacca gcgttttaaa ttcgtgaacc
     8461 gccgcacccg gctcgcgcga cgcggcgtag cggctcatcg gtgcgaaaaa ggcgagggtg
     8521 ctgtagcccc agaaattgag cagctccttg cgcgccgcgc cttccagaaa aaactccagt
     8581 tcgttgaact cgaaaaccgg cagaaattca acagcggtaa tgccgagcga ttgcaggtag
     8641 gggatcttat cgatgaaatc aagatagctt ccgccgccgt caccgcgcgt aaagccgcgc
     8701 agatgcgcct cataaatcac ggttttttcg agcggcgtat gcggatgccg gtcgccgtgc
     8761 cagtcgaacg actcgtccgg aatgacgcac ttcgggaact gtttcccggt gcggatgtgc
     8821 gctcccggtt cgaggccggc cgtttcgccc caggcgcgcg ggaaatggac ggcgcgggca
     8881 tacggatcga gcagccactg ctcggggacg gcgtcggtgc ggtagagata aaaggcgccg
     8941 gggcggaggc cgggaacctt gacgcgccag agcggaccgc ccgacatctc gatctcgcgg
     9001 gcgggcgttc ccgcatccgt cgaatcaaac agaagtagcc acacgcggtt ggcgctgctg
     9061 tgaacggcga attcgatttc gtctttgcgg atgtaaacgc cgggtttcat ggcgggcagt
     9121 ctacgcccga ccccgggccg atttcaatct ttgccgcttc caaaggtcgc tgccccgcag
     9181 ggcggggcct ctctcgcttc gctcgggtcc aatcagtaga aaccggcgcg gttgcaggta
     9241 gagctcggtc atcttattat atcgaacgtc ctggatcacc ctgagcgcgc ggagcaagcg
     9301 atagggtgaa cgctcgggtt ggcctatgtt ttagttggtg cagtgtcatt tttgtgttgg
     9361 ggttcgtaat gccagaagtt tatgggggac gaaggcttta atgtcttcaa gcaacgacaa
     9421 gagtctaaac tcacatatag tttcctcttt ttgcagccgc tgttgaaatg atcaagaacc
     9481 tcgaatcgga atttttctct gtttgttggg cataatttgt cctcatcgaa taaaccaagg
     9541 tctggggtct tctgggactg ccaaagtttg aatagtgttg attggattgg ctgttattca
     9601 aatttggttg gctggaaaat aacaccgagt tctgatttcg aaagatacta tttgaaacga
     9661 tctgtgtaac tgacatcctg ttagtcccca tatatttatg ttttatcccg gcgcgaggcg
     9721 gattagcatt tctccctatg aacagttttg aactcaaggg tttggtcgtt ccggtgccga
     9781 ctccgttcgc ggagagcggc gcggtggatt tcggggcgtt tctggcgcat ctcgactggc
     9841 tggcggaatg cggcgtacgc aatctgctgg
//
LOCUS       DAOPZO010000072         9757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_8180_length_9757_cov_10.431251, whole genome shotgun sequence.
ACCESSION   DAOPZO010000072 DAOPZO010000000
VERSION     DAOPZO010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9757
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8180_length_9757_cov_10.431251"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PKI68_05940"
     CDS             complement(<1..893)
                     /locus_tag="PKI68_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNX53266.1"
                     /translation="MKNEKIRNVAIIAHVDHGKTTLVDQIIKQAHILRDNEAFQTCLL
                     DSNDLERERGITILSKNISIVYNGVKINVIDTPGHSDFGGQVERVLNLADGVLLLVDS
                     AEGPMPQTRFVLDKALELNLKPVVIINKVDKPDARCDEVHNMVFDLFVELEANDDQLD
                     FPVLYASGRDGWAVTDLNDPRDSIKPLMEAIIKHIPGPPVKEGPVQMQATTLDYNDYV
                     GRIGIGRVYRGTLDLKTPVCIIKRDGTVRPVQLKELQTFEGLGRVKTTGIPCGDLCAI
                     SGIMDIDIGDTIAHAEHPEALP"
     gene            complement(1005..1553)
                     /gene="msrA"
                     /locus_tag="PKI68_05945"
     CDS             complement(1005..1553)
                     /gene="msrA"
                     /locus_tag="PKI68_05945"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNX53267.1"
                     /translation="MRALLTSILLAVIAGTAPAEEGIMKTEKAVFAAGCFWGVESAFR
                     QVAGVIDTQVGYTGGKTADPTYKEVCIDTTGHAEAIEITFDPSKVSYAKLLDLFWRMH
                     DPTQVNRQGPDTGTQYRSAIFYYSPEQKAAAESSKAALEKSGKYKKPIATQILPAGPF
                     YRAEEYHQRYFEKNGGPACHIF"
     gene            complement(1615..2112)
                     /gene="tpx"
                     /locus_tag="PKI68_05950"
     CDS             complement(1615..2112)
                     /gene="tpx"
                     /locus_tag="PKI68_05950"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNX53268.1"
                     /translation="MAKITFKGSPITTAGELPAKGSAAPDFTLVKQDLSETVLADYKG
                     KRLVLNIFPSIDTGVCAMSVRKFNEKAAALKNAAVLCVSMDLPFAQSRFCGAEGIKNV
                     VTASAFRSAFGDHYGVIIADGPLTGLLSRAVVVINESGKVIYTEQVPEITQEPNYDAA
                     FAALK"
     gene            2219..3601
                     /gene="der"
                     /locus_tag="PKI68_05955"
     CDS             2219..3601
                     /gene="der"
                     /locus_tag="PKI68_05955"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNX53269.1"
                     /translation="MKTTSQQRIIAIVGRPNIGKSALFNRIVGRRVSIVHEECGVTRD
                     RVVCEAVWNEQYFDVIDTGGLATMDSGSAHNVIVDSTNKQVAVAIEDAAAIIFVADIT
                     TGIVPMDEEVARLLHRADRPVFIAVNKADTPARDEQAPEFSKLGFPVFPVSALHNRGI
                     DALLQAALAKLPAAPPREKRTPLRVAVVGRPNSGKSSYINRLLNSERVIVSEIPGTTR
                     DSIEIPFTIGSGPHARHYLLIDTAGVNRRNRLSGAVEHYSNVRAEESIQSADVVILLM
                     DATEGPKLQDKKLAAKIIEAQKGCILAVNKWDVAEGQEVTQTKYEPALRKEMPFLDFA
                     PLLFLSAKSGYNIRRSVELIDYISDQVQIKLSTGVLNRILQDAVQKNPPPSVNGRPLK
                     IYYATQAGVKPVRVKLFVNNTLRATSSYKRYLDHEIRKAFGLEGAPIELKFVPRPRPD
                     LEEKKKQRKV"
     gene            complement(3609..5489)
                     /locus_tag="PKI68_05960"
     CDS             complement(3609..5489)
                     /locus_tag="PKI68_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNX53270.1"
                     /translation="MKNNFFDVIVVGGGHAGCEAALASARLGARTALLSLEKKFIGRM
                     SCNPSIGGIAKSHITCELDALGGEQARNTDYTGIQFRTLNTRKGPAVQAFRAQCDKAL
                     YPVRMQAVVDQQKNLVVLEAVVSALWIESGRLCGVILDTGEKLAGKTVILTAGTFMRG
                     RVMIGQTIIHEGRWGERSVDDLSMSLKSLGFELGRMKTGTPPRIHKDSIDYSKMAIQP
                     GDAPPPFFSRAARQDYEKFHVEPLRQGTATDVPHGTALRPWTPGTDQIPCWLTHTNEK
                     THQIIADNLKKSSLYGGMIEGAGARYCPSIEDKVVKFPQRTSHHVFIEPEGRNNVRIY
                     PNGTSNSLPEDVQLEMIHSIAGLEKAVFIRPAYAIEYDYAPPTQLFHTLETKTVENLY
                     FAGQINGTTGYEEAAGQGFIAGVNAARKVLGLPEFILGREEAYIGVLIDDLVLKGTDE
                     PYRMFTSRAEHRLTLRQDNVWFRLHARAKELGIVSAEDLAKTVQMQAQIDQEIDRLRK
                     KFYAGSSLAQTLCRQDNHYAGLPGEKPDLPPEVIEQVEISIKYEGYIKREAGRIVDFQ
                     SLEKQRIPADFDYDAIIALRFESREKLKNIRPENLGQASRISGVNPADISILSVWLKA
                     GK"
     gene            5572..5886
                     /locus_tag="PKI68_05965"
     CDS             5572..5886
                     /locus_tag="PKI68_05965"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNX53271.1"
                     /translation="MKTNLFDRIPEHAAKELFTELLSEGTVRIERIVSFGQASPAGFW
                     YDQPENEWVLLLEGSAQLQLDGKTVDLASGDYLNIPAGMRHRVEKTAENTRTVWLAVF
                     YK"
     gene            complement(5963..7057)
                     /gene="prfA"
                     /locus_tag="PKI68_05970"
     CDS             complement(5963..7057)
                     /gene="prfA"
                     /locus_tag="PKI68_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNX53272.1"
                     /translation="MINKAYLENLRRRISGLETEMSAPGVASNPQKMQALMHDYAHQK
                     RVAGAAEIFLKLQDAAAESRAMLADTSTDAELREMAEAELADVEAKLPEAEKAVKVAL
                     LPPDPSDSKNVIMEIRAGTGGDEAALFAGDLYRMYSRYAENNGFKISVLDVSPSETGG
                     YKEVVFSVEGEMVYKKLKYESGTHRVQRVPATETQGRIHTSAATVAVLAEAEEVDIEI
                     KNEDLRIDTYRSSGSGGQHVNTTDSAIRITHIPTGVVVQCQDERSQHKNKAKAMRVLR
                     SKLYEARQQQIEAEQSAARRSQVGSGDRSEKIRTYNYPQNRLTDHRINLTLYKLDRVM
                     EGDLEDVFNALYEHDIELKVKAHTDKTAQG"
     gene            complement(7114..7311)
                     /gene="rpmE"
                     /locus_tag="PKI68_05975"
     CDS             complement(7114..7311)
                     /gene="rpmE"
                     /locus_tag="PKI68_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014697411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNX53273.1"
                     /translation="MKKEIHPEYTDAEISCACGSVIKTRSTVKKMHVNLCSACHPFFT
                     GTAKMLDTEGRVDRFRKRYGK"
     gene            complement(7380..8297)
                     /locus_tag="PKI68_05980"
     CDS             complement(7380..8297)
                     /locus_tag="PKI68_05980"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX53274.1"
                     /translation="MASSCKYLFGPVPSRRLGRSLGIDVTPFKTCSFDCVFCQCGCTT
                     RLVTERSEFVPFEEVCAEIEQWLKADGQADVITFAGSGEPTLYSRLGELIDFIKARTK
                     IPVIVLSNGTLLHRITVRDELLKANVVKVSLSAWDDVSFKKINRPARGLSFDQLLAGL
                     RDFRTVFRGELWLEVFLMEGINTRPEHVEKIAAAAAGIRPDKIHLNTAVRPPAEAAVR
                     PVAKEKLEAFCGLFTPRAEVIASFSAATASAAGLSVEKLIDLIRRHPATAAQLAENFG
                     AEPAAILTLLGDPRLQTEVRNGETYYKCR"
     gene            complement(8301..9530)
                     /locus_tag="PKI68_05985"
     CDS             complement(8301..9530)
                     /locus_tag="PKI68_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53275.1"
                     /translation="MLRKLVPVLLAAHLICSAAPPEVEQQARALQTVLRQADDAYFNQ
                     HQPVMSDTAYDALRNQYDRLMADYPELSAGNPVGAPVAKSSRVAHAAPVLSLEKVYSD
                     NDLTRFLRKTGTNLLYCVEPKLDGLTVVLRYRNGLLVQALTRGDGKTGADVTSAVLAS
                     GAVPAALTGAPAKLDVRGEAHLPLSAFEALNRRRAENGQEPLKSPRNIASGTLMLTDC
                     AEIARRGLEFQAFDLLAADPMPATHTEALALLKKNGLPVIESVTVPSVDVLATVENMN
                     RRRAEFPFVTDGVVIKVNDLAVRNRLGATSHHPRSAIARKYEETPVQTRLLAVDWSRG
                     ASGKLTPVAHFEPVEIQGALVQRASLYSLEHLRAMDLKIGDWMNVIRSGGTVPEITGI
                     CPERRTGRETAIPDPVE"
BASE COUNT         2169 a         2599 c         2686 g         2303 t
ORIGIN      
        1 ggcagcgctt cgggatgttc ggcgtgggca atggtgtcgc cgatgtcgat gtccatgatg
       61 ccggagatgg cgcaaaggtc accgcacggg atgccggtcg tttttacgcg gccgaggcct
      121 tcaaaggttt gcagttcttt gagctgcacc gggcggacgg ttccgtcgcg tttgataatg
      181 cagaccggag ttttcagatc aagtgtgccg cgatagacgc gaccgatgcc gatacggccg
      241 acatagtcgt tgtagtcgag cgttgtggcc tgcatttgaa caggaccctc tttgaccggc
      301 gggccgggga tgtgtttgat gatggcttcc atcagcggct tgatggagtc gcgcggatcg
      361 ttcagatcgg taacagccca gccgtcgcgg ccgctggcgt agagtaccgg gaagtcgagc
      421 tggtcatcgt tggcttcgag ttcaacgaaa aggtcgaaga ccatgttgtg cacttcgtcg
      481 cagcgcgcat ccggcttatc cactttatta atgatgacaa ccggcttgag gttcagttcg
      541 agcgccttgt cgagtacgaa gcgcgtttga ggcatcggcc cttcagcgga gtcgaccagc
      601 aaaagaacgc cgtcggcgag attaagtacg cgttcgacct gtcctccgaa atcgctgtgg
      661 ccgggagtat cgatcacgtt gatcttcaca ccgttgtaaa cgatgctgat gtttttggag
      721 agaatggtga tgccgcgttc gcgttcgaga tcgttgctgt cgagcaggca ggtctgaaag
      781 gcttcgttat cgcgcagaat gtgggcctgc ttgatgatct ggtcgaccag cgtggtttta
      841 ccgtggtcaa cgtgcgcgat gatcgcgaca ttacgtattt tctcgttctt cataggtgct
      901 ccggacataa gggcggacaa tttacgcacc tgccgggaaa aagcaatccc gccgacgtta
      961 aatctgcgtt tggcaaaatg gctctaatta tagaaaggcg cagatcaaaa gatatgacag
     1021 gcgggaccgc cgttcttttc gaaatagcgc tgatgatact cttcggcgcg gtaaaacggg
     1081 ccggccggta aaatctgggt cgcgatcggc tttttatatt tgccgctttt ttcgagtgcg
     1141 gctttagagc tttcggctgc cgctttctgc tccggcgagt aataaaagat tgcggaacgg
     1201 tattgggtgc cggtatccgg cccctgccgg tttacctgtg tcggatcgtg cattcgccag
     1261 aagagatcca gaagtttcgc ataagaaact tttgagggat caaacgtgat ctcaatcgct
     1321 tcagcatgtc cggtggtatc gatgcagact tctttgtagg tcggatctgc ggtttttccg
     1381 ccggtatagc cgacctgcgt atcaattacg ccggcgacct gccggaacgc ggactcaacg
     1441 ccccaaaaac atccggcggc aaacacggct ttttcggttt tcataatgcc ttcctctgcc
     1501 ggagcggttc cggcaattac ggcaagcaga atgctggtca gtagagccct catcccaata
     1561 atagacacct gattaagcga acagctcaat tcagcagccg tgttttccgc cagactactt
     1621 cagtgcggcg aacgcggcgt cgtaattcgg ctcctgcgta atttccggaa cctgttcggt
     1681 ataaatcact ttgccgcttt cattgatgac gactactgcg cgcgaaagca atccggtcag
     1741 cgggccgtca gcgatgatta caccgtaatg gtctccgaat gcggaacgga atgccgaagc
     1801 ggttacaaca tttttaatcc cttccgctcc gcagaaacgg ctctgggcaa acgggagatc
     1861 catggacacg caaagaaccg ccgcgttttt cagcgcggcc gccttttcgt tgaatttgcg
     1921 gacggacatg gcgcagacgc ccgtgtcgat actcgggaaa atattcagca caagccgctt
     1981 tcctttataa tcggccagta cggtttccga aagatcctgc ttaacgagtg tgaagtccgg
     2041 tgccgcgctg ccttttgccg gcagttcgcc tgcggtagtt atcggcgagc ctttgaaggt
     2101 aattttagcc ataggaaccc tcctttttta aaacaatgcc tgatgcggat ttatacgaag
     2161 agggttgctt ttgcaaggcg caacccgttc aatgtgcgcc ttattagagg attatgaaat
     2221 gaaaacgact tcccagcaga gaattatagc cattgtcgga cgtccgaata tcgggaaatc
     2281 ggcgctgttt aaccggattg tcggccgtcg cgtctcgatt gtacatgaag aatgcggcgt
     2341 tacgcgcgac cgtgtagttt gcgaagccgt ttggaatgaa cagtatttcg acgtcatcga
     2401 caccggcgga ctggccacca tggattccgg ctcagcgcat aatgtgattg tggacagcac
     2461 caacaagcag gtcgccgtcg cgatcgaaga tgccgcagcc attatttttg tggcggatat
     2521 caccacagga attgttccga tggatgaaga ggtcgcccgg ctgcttcacc gcgcggaccg
     2581 gccggtattt atcgctgtga acaaagccga tacacccgct cgcgacgaac aggcgcctga
     2641 attttcaaaa ctcggctttc cggttttccc ggtttccgcg cttcataatc gcggcatcga
     2701 cgcattgctg caggccgcat tggcgaagct tcccgcagca ccgccgcgag aaaaacgcac
     2761 accgcttcgc gtcgctgttg tcggacgccc taattcgggt aaatcctctt atatcaacag
     2821 gttattgaat agcgaacgag tgatcgtttc tgaaattcca ggcacaacca gggacagcat
     2881 tgaaatcccc ttcacgatcg gcagcggtcc gcatgcgcgg cactacctgc tgattgacac
     2941 cgccggcgtc aaccggcgca accggctttc cggcgcagtt gagcactata gtaatgtgcg
     3001 tgcagaagag agcatccaga gtgccgacgt tgttattctg ctcatggacg ccaccgaagg
     3061 cccgaaactg caggacaaaa aactggccgc caaaatcatc gaagcgcaaa aaggatgtat
     3121 tctggcggta aataaatggg atgtcgctga aggacaagaa gttacacaaa caaaatacga
     3181 acccgcgctg cgcaaagaaa tgccgtttct tgactttgcc ccgctgctct ttctctcggc
     3241 gaaatcgggc tacaatattc gccgcagcgt agaactgatc gactacattt cagatcaggt
     3301 tcagatcaag ctttccaccg gggttctaaa ccggatttta caagatgccg tgcagaaaaa
     3361 cccgcccccg agcgtcaacg gacggccgct taaaatttat tatgctacgc aggccggcgt
     3421 gaagccagtc agagttaagc tgtttgtaaa taacacctta agagcaacat cgtcatataa
     3481 acggtatctg gatcacgaaa tccgcaaagc tttcgggctc gaaggcgcac cgatcgaact
     3541 gaaatttgtc ccccgccccc gtccggatct ggaagaaaag aaaaaacaac gcaaagtgta
     3601 atccgtcttt atttcccggc tttaagccat accgacagga tggaaatatc ggccggattg
     3661 acgccggaga tacgtgaggc ctggccgagg ttttccggac ggatgttttt cagcttttcg
     3721 cgcgattcaa aacgcagtgc gatgatggca tcataatcaa aatcagccgg aatgcgctgc
     3781 ttttccaacg actggaagtc aacgatacgc cccgcttcgc gcttgatata gccttcgtat
     3841 ttgatggaaa tttcaacctg ttcaatcact tccggcggca gatccggctt ttcgccaggc
     3901 agtccggcat aatgattgtc ctgccggcag agagtctgtg ccagcgaaga ccccgcgtag
     3961 aactttttcc gcaaccggtc tatctcctga tcaatttgcg cctgcatctg aacggttttc
     4021 gccaaatctt cggcagacac gattcccaat tcttttgcgc gtgcatgaag ccggaaccag
     4081 acattatcct gtcgaagtgt cagccggtgc tcggctcgcg aggtaaacat ccggtacggc
     4141 tcgtcggtgc cttttaaaac caaatcatca attaaaacgc cgatatacgc ctcttcccga
     4201 cccagaataa attcaggaag gccaagaact ttacgcgccg cgttaactcc ggctataaat
     4261 ccttgtccgg cggcctcttc atagccggta gttccgttga tttgacctgc aaaataaagg
     4321 ttttcaaccg tttttgtttc cagagtatgg aaaagctgcg tgggcggcgc atagtcatat
     4381 tcgatggcgt atgcaggacg gataaataca gctttttcca gtccggcgat ggaatgaatc
     4441 atttccagct gcacatcttc cggcagactg ttggaggttc cgttgggata aattcgcacg
     4501 ttattacggc cttccggctc gataaagaca tgatgcgagg tccgctgcgg gaatttcaca
     4561 actttatctt cgatcgacgg gcaataccgg gccccggcgc cttcgatcat tccgccgtat
     4621 aaagaacttt ttttcaggtt atcagcgata atctgatggg ttttctcatt ggtatgagtc
     4681 agccagcacg gaatttgatc cgttcccggt gtccaagggc gcaacgctgt tccatgtgga
     4741 acatctgttg ctgttccttg gcgcaacggt tccacgtgga acttctcgta atcctgccgt
     4801 gcggcacgcg aaaagaaagg cggcggcgca tcgccgggct gaatcgccat tttcgaataa
     4861 tctatgctgt ctttgtgaat acgcggcggt gtgccggttt tcatacggcc caattcaaat
     4921 ccgaggcttt tcaacgacat ggaaagatcg tctacagacc gttcacccca gcgaccttcg
     4981 tgaatgatgg tttgaccgat cattacacgt ccgcgcatga aagttccggc agtcaatata
     5041 acagtttttc cggcgagctt ttcaccggtg tccagaatca ctccgcataa ccggcccgat
     5101 tcgatccaaa gagccgagac gacagcttca agcacgacta gatttttctg ctgatcaacg
     5161 acagcctgca ttcggacggg gtagagggct ttgtcgcact gcgctcgaaa agcctgaact
     5221 gccggacctt tgcgggtgtt cagcgttcga aactgaatgc cggtataatc ggtattgcgc
     5281 gcctgctccc cacccaatgc gtctaactcg cacgtaatat gagatttagc gataccgcca
     5341 atcgatggat tgcaggacat ccgtccgata aatttctttt caagactgag cagggcggtt
     5401 cgggcgccga gccgtgcgga ggccagagcg gcctcacaac ccgcgtgtcc gccgccgact
     5461 acgatcacat caaaaaaatt gtttttcatg aggtttgaat gatggagcgg cgggaaaata
     5521 aaaccagatt tttcttcagc aaccgaggaa ggaaccctgt attctgcgga gatgaaaacc
     5581 aacctgttcg accggattcc tgaacacgca gcaaaagagc tgtttaccga actgctgtcg
     5641 gaagggactg tgcgcattga gcggattgta tccttcggcc aggcttctcc cgcgggattt
     5701 tggtatgacc agccggagaa tgaatgggtg ttgctgctgg aaggttctgc gcaacttcaa
     5761 ttggatggga aaacggtcga tttagcttcc ggtgactatt taaatattcc ggccggaatg
     5821 cggcatcgtg tggaaaaaac cgctgaaaac actcggacgg tctggctggc tgttttttat
     5881 aaataggcgg tttttttgat aaaaatgcct taaatcagct gataaatctg atttttgtgt
     5941 aaaaaatgac gatttttgaa gatcagcctt gggcggtttt gtcggtatga gccttaacct
     6001 tcagctcgat atcgtgttca taaagcgcgt taaaaacgtc ttcaaggtcg ccttccataa
     6061 cgcgatcgag tttataaagc gtcaggttga tgcggtgatc ggtcagccgg ttctgagggt
     6121 agttgtatgt gcggatcttt tcgctgcggt cgccggagcc gacctgcgaa cgccgggcgg
     6181 ccgactgttc ggcttcgatc tgttgctgcc gcgcttcata aagctttgaa cgcagaaccc
     6241 gcatagcctt ggctttgttc ttatgctgcg agcgttcatc ctgacactga accaccacgc
     6301 cggtcggaat atgagtgatg cggatagccg agtcggtggt gttgacatgc tgtccgcctg
     6361 atccgctgga acggtaggtg tcgatgcgca aatcttcgtt tttgatttcg atatccactt
     6421 cttcggcttc ggcgagcacg gcgacggtgg cggcggaagt gtgaatacgg ccttgtgttt
     6481 cagtggcggg gacgcgctgt acgcgatggg taccgctttc atacttcagt tttttataaa
     6541 ccatttcgcc ttcgaccgag aaaacgactt ctttgtaacc gccagtctcc gatggactta
     6601 cgtcgagaac tgaaatttta aacccgttat tttcagcata acggctgtac atgcggtaaa
     6661 ggtcgccggc aaacagcgcg gcttcatcgc cgccggtgcc ggcgcggatt tccatgataa
     6721 cgtttttcga gtctgacgga tcgggcggaa gcagagccac cttgaccgct ttttctgctt
     6781 caggcagttt ggcttcgacg tcagccagct cggcttcagc catttcccgc agttcggcat
     6841 ccgtggatgt gtctgcaagc atggcgcggc tttcggcggc ggcatcttgc agtttcagga
     6901 aaatctcagc agccccggcg acccttttct gatgcgcata atcgtgcatc agtgcctgca
     6961 ttttctgggg attggacgca acgcccggcg cggacatttc cgtttccagc ccggagatgc
     7021 gcctgcgcaa attttccaga taagctttat taatcatggc gggacacaaa aaggccgggc
     7081 tctgaaaaca gagtccggcc taaacgtgcg tcgttacttg ccgtagcgtt tgcggaagcg
     7141 gtcaacgcga ccttccgtat caagcatctt ggccgttccg gtgaagaacg gatggcaggc
     7201 ggagcagagg ttgacgtgca ttttcttaac ggtcgagcgg gttttaatta cgctgccgca
     7261 ggcgcacgaa atttctgcgt cggtatattc aggatgaatt tcttttttca ttttttaacc
     7321 tagtccttgt aaaagagcgg gtcttttaat cataacgggc cgcgcggcgc aagccctttt
     7381 taccggcact tataataggt ctcgccgttg cgcacctcgg tttgcagccg cggatcgcca
     7441 agcagcgtca aaatagccgc cggttcagcg ccgaagtttt ccgccagctg ggcggccgtc
     7501 gccggatgac ggcggatcaa atcaatcagc ttttcaacgg acagcccggc ggcggaagcg
     7561 gttgcggcgg aaaaagaagc aattacttct gcccgcggcg tgaagagccc gcagaacgct
     7621 tccagttttt ctttggcgac cggccggacg gcggcttcag ccggcgggcg gacggcggta
     7681 ttcagatgga ttttatccgg ccggattccg gccgccgccg ccgcgatttt ctccacatgt
     7741 tcaggacggg tattaatacc ttccattaga aaaacttcca accacagttc gccgcggaaa
     7801 acagtccgga aatcacgcag acctgccagc agctggtcga aagaaagtcc gcgggcaggg
     7861 cggtttattt ttttaaacga aacgtcgtcc catgcgctca ggctgacttt caccacgttg
     7921 gccttcagga gttcgtcgcg taccgtaatc cggtggagca gggtgccgtt cgaaagcaca
     7981 atgaccggaa tttttgtacg cgccttaata aaatcaatca gctcgccgag ccgcgaataa
     8041 agcgttggct cgcctgaacc ggcgaaagta atcacatcag cctgaccgtc ggctttcaac
     8101 cactgttcga tttccgcgca gacctcttcg aaaggaacaa actccgaacg ttccgtaaca
     8161 aggcgcgttg tgcagccgca ttggcagaaa acgcagtcga acgaacaggt tttaaacggc
     8221 gtcacgtcga taccgagcga ccggccgagg cggcgcgacg gaaccggacc gaagagatat
     8281 ttgcaggaag aagccataat ttattccacg ggatccggga ttgcggtttc gcggccggta
     8341 cgccgctccg ggcagattcc ggtaatttca ggaacagttc cgccggaacg gattacattc
     8401 atccaatcgc cgatttttaa atccatcgca cgcagatgtt ccagactgta aagtgacgcg
     8461 cgctgaacca gcgcaccctg aatttcgacc ggctcaaagt gtgcaaccgg cgtcagtttg
     8521 ccggaagcgc cgcgcgacca atcaacggcc agcagacggg tctgaaccgg cgtttcttca
     8581 tacttgcggg cgatggcgct gcgcggatga tgcgacgtgg cgccgagccg gttccggacg
     8641 gccaggtcgt tcaccttaat gacgacgcca tcggtcacga acggaaactc cgcccggcgg
     8701 cgattcatat tttctactgt cgcaagcaca tccacggacg gaactgtaac actttcgatt
     8761 accggcagcc cgtttttttt cagcaaagcg agggcttctg tgtgcgtggc cggcatcggg
     8821 tctgccgcca gcaggtcaaa ggcctggaat tccaggccgc gcctggcgat ttcggcgcag
     8881 tcggtcagca tcagcgttcc ggacgcgata ttacgcggac ttttcagcgg ctcctgaccg
     8941 ttttcggctc gccgtcggtt cagcgcctca aaagcggaca gaggaaggtg cgcttcgccg
     9001 cgcacatcca gttttgccgg agcgccggtc agcgcggcgg gcaccgcgcc ggaagcgagt
     9061 acggcggacg ttacatctgc gccggtcttt ccgtctccgc gcgtcagcgc ctgcaccagc
     9121 agaccgttgc ggtaacgcag taccaccgtc agcccgtcga gtttgggttc aacgcaatag
     9181 agcagattcg ttccggtttt ccggagaaag cgcgtcaggt cgttatcgga atagactttt
     9241 tcaaggctca gcaccggcgc agcgtgcgcg acacggctgg atttagcgac cggtgcgccg
     9301 accggatttc cggcggacag ttccggataa tcggccatca accggtcata ctggttgcgc
     9361 aaggcatcgt aagcggtgtc gctcataacc ggttgatgct gattgaaata agcgtcgtcg
     9421 gcctgtcgga gaaccgtttg cagcgcgcgg gcctgctgtt cgacctccgg cggcgcggcg
     9481 gagcagatca gatgggcggc gagaagaaca ggcaccagtt tacgcagcat agcaggctcc
     9541 ctttttgaag tttccggggt ttgtaaactt gcaaagtgtt tctaacggct gcccgccgca
     9601 aaagcgaaac atttttcggg tgcattccgc gccgttcgcg gctacaaact tctgccatga
     9661 gcaagatcgg catcattgac tacggcatgg gcaacctcgg cagcgtttcc aacgcctgca
     9721 aatttctcgg gctggacgcg gaaatcatct ccgcgcc
//
LOCUS       DAOPZO010000073         9754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_8182_length_9754_cov_10.994226, whole genome shotgun sequence.
ACCESSION   DAOPZO010000073 DAOPZO010000000
VERSION     DAOPZO010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8182_length_9754_cov_10.994226"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="PKI68_05990"
     CDS             <1..1345
                     /locus_tag="PKI68_05990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HNX53276.1"
                     /translation="KMERISASMRVNLLTASITVMITAGTVQAVDLLTAYRQAAESSP
                     LVKQASARWQAEQSGKDIARSALAPRLTVEGAASQNQLDLTGFGAAPINEEYNPSSYS
                     VTLTQPLINGAAWSTFQASRSKAQSYEAGLLAVKQDLILQTADAYFAVLRAEALERTA
                     LSQQTLLQKICDRAEGERRAGTGDMIAVQEARARLDAAQAELLSARNGVQLARRALEK
                     LTHQPVETLTDLETLEPQGPVPALMEEWVKSAEVSQPVLEQARQELQASRYMIAAASR
                     GNWPVVSLNAQYIRADGSFMPAIERRESLVGVSAVWPLFEGGRVRASKARAEALARVS
                     QYGLEALTDNVRLETQRAFLNLENSVAQLTAARRALESAQTALKATQKGYEIGSRSVV
                     DVLDSARRQAAAESDYYLALYSQVLCRMQLKASAGVLSENDVHAINQLLTVKPGV"
     gene            1354..2355
                     /locus_tag="PKI68_05995"
     CDS             1354..2355
                     /locus_tag="PKI68_05995"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX53277.1"
                     /translation="MKKVLPKAVPALLLIAGIGGVLIFRHEQEISKRLTFYGNVDIRQ
                     IQTAFYSTGRIQQMLAREGDAVTNGQLIAELDATRYEAAAAKAEAQVAMQQQVVARML
                     AGSRPEEIVQAQARVREAEAALTDALQKQVRAQALAKEEYVSQQQLDDARTAVDTKRA
                     NLEAVRQSAELAVLGPRKEDIAAAQAQLSVYEAALKLAKQELADSKLYAPADGIIQNR
                     ILEPGDMASPQSPVYTLALNNPVWVRAYVPEPDLGKISAGLRAEVRTDSFPGKVYEGW
                     VGYISPSSEFTPKQVETTDLRTRLVYQVRIYVKNPQNELRLGMPATVKILTGSAQEN"
     gene            2358..4103
                     /locus_tag="PKI68_06000"
     CDS             2358..4103
                     /locus_tag="PKI68_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX53278.1"
                     /translation="MPVPADSSTVLELRDVTKRFATSAALNGVSLSVRRGSVTGLIGP
                     DGAGKTTLMRLIAGLLVPDRGRISVLGIDAVQEPLKVQHVIGYMPQRFGLYEDLTVEE
                     NLNLYADLQGLPDAARPARYAELTKMTGLAPFTKRLAGKLSGGMKQKLGLACVLVRPP
                     QLLLLDEPTVGVDPVSRRELWSIVYRLVQQEKMTALLSTAYLDEAERCNEVILLHKGE
                     LLGQQSPAEFSRQVDGRSFQATLPGLNKRSLQGRLARAPGVLDATIQRTGVRIVMKSA
                     TAPDLAGLPHGIRIEPVPARFEDSFISLLREKDGHSAPPDSPSQPFAPTGGDMITVEQ
                     VTKRFGSFCAVRGISFSVKEGEVFGLLGANGAGKSTTFRMLCGLLPATDGKLSVAGLD
                     LRHAPAKARARIGYMAQKFSLYGDLSVQENLRFFSKAYGLRDREQRERIEWALREFEL
                     TDYAASTSRELPLGYKQRLALAAALMHRPKIIFLDEPTSGVDPLARREFWHRISGLAE
                     EGVTVLVTTHFMEEAEYCDRLVIMAEGDILAEGTPDELRARVRSEQLADPTMEDAFIH
                     LIEEHEAAGRPDAVS"
     gene            4090..5241
                     /locus_tag="PKI68_06005"
     CDS             4090..5241
                     /locus_tag="PKI68_06005"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX53279.1"
                     /translation="MRFRKQPDPEKRAAQMRLRGMLRKEALQILRDPSSIAIAFVLPI
                     VLLFLFGYGISLDAKHVPMAVVIESPSAEAADFAASFEQSDYFSPQRFGTIQDAEQAL
                     IRHRVNGIVWLRNDFEEKLLSGASAPVGVIINGVDANTARIIEGYVNGAWSAWLQQRA
                     TAQAETLKLPVRLQPRVWFNAELRSRNFLIPGLIAVIMTLIGALLTAMVVAREWERGT
                     MEALMVTPVTAKEILLGKLLPYFVLGLGGLALSVGMAVWLFGVPLRGSLLVLFLTSAL
                     FLIAALGMGLTISTAARNQFVAGQIAIIATFLPAFMLSGFIFDINSMPAAIQIVTHLL
                     PARYYVAILQTVFLAGNVWNVLLLNSLALMLFCVFFLGLARRRTVKRLE"
     gene            5245..6354
                     /locus_tag="PKI68_06010"
     CDS             5245..6354
                     /locus_tag="PKI68_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX53280.1"
                     /translation="MWTHLKALVMKELLALLKDKKSRLVLIAPPLIQLFVFGYAATFD
                     LNHIRYAVLNEDRGAASRELLARFEGSGTFERVAQLDGDAQIEPVIDNKRALLVVRFP
                     SDFSRNLAQGRPADVQINVDGRNSNSAMLALNYVQSIVSSFNAVWMKEHGGRAPPANL
                     VVRPWYNPNLVSRWFIVPGIIGLLTLLVTLLITALSVAREREEGTFDQLLVTPLRPFE
                     ILLGKAIPGVLLGLAEASAILLIIVFWFKIPLLGSLAALYLGLLLFLLSSIGVGLMIS
                     SLTVTQQQGLLGAFLFLVPSIILSGFSTPIANMPLYLQKLTLINPLRYFIVILRSVFL
                     EGATVPLLWPQYWPMALIGCICLFMAAILFRRRMV"
     gene            complement(6357..6824)
                     /locus_tag="PKI68_06015"
     CDS             complement(6357..6824)
                     /locus_tag="PKI68_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX53281.1"
                     /translation="MKSGTEKSAFWIRPAGTDDLAAMMRLLQELFTIESEFEFDAVKQ
                     RCGLQMLLESASASVWVAERHGRVAGMVTVQMVISTATGGFSGLIEDLVVSQICRRRG
                     LGKALLAAAVKWAREQGATRVQLLADGRNVPALIFYRKQGWKQTNMIALRNFC"
     gene            7153..7923
                     /locus_tag="PKI68_06020"
     CDS             7153..7923
                     /locus_tag="PKI68_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX53282.1"
                     /translation="MKNVLSITVLSLLVVATGCRSPKMPLTPAQQGAVVGGSGGAVVG
                     AVMAQNANASLSSWQGAGAGAVAGVAAGALVGDALDEYAYDNASGMKDKDAQINDLQR
                     QLADAEAKLRGQGMDNVKVEAVNGQLRFTILNEVLFESGKAELKTGSKSTIDSVLNII
                     QSDFANRDITIEGHTDSDPIMKSKWKDNWELSYNRSMAVLYYLMNEKGIPAERLKAVA
                     CADSQPVAPNDSAANKRLNRRAVIVVMPAKDSIIVEKK"
     gene            7990..8643
                     /locus_tag="PKI68_06025"
     CDS             7990..8643
                     /locus_tag="PKI68_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53283.1"
                     /translation="MFLLCGCATSVESPIRKPQPCAAPQPLRVAVLPPRIHPDLLPEN
                     LKSEKPLYKQMMDGRGAVVTSGSASKDIGLEIVSTLTQAGKYGRIFTVETEEEARQLG
                     ADALLTVTVWDYRTVQLGSNKKYPLIVALGLLMPQYWIRWQTIEARFEWEVKLTSLAD
                     ASTLYRNRLKREYTSSVRSASGHYFTDKMLSFLQNRAAPDFIGELFALEMLTPPAEK"
     gene            8683..9249
                     /locus_tag="PKI68_06030"
     CDS             8683..9249
                     /locus_tag="PKI68_06030"
                     /inference="COORDINATES: protein motif:HMM:NF015142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNX53284.1"
                     /translation="MKPFCALFLAVNIGLIACGAGADTNRPPSWATPIELAGVPNLHK
                     VSDTLYRSAQPTAEGMKNLEKMGIKTVINLRSFHSDRDELRGTSLSGEHLFMKAWHPE
                     QKEAERFLQIVTDPARTPVLVHCQHGADRTGTLCAIYRIAVQNWPKDAAIREMTGGGF
                     GFHEIWSNLPAWIEQLNINQLNCKPPVQ"
     gene            complement(9257..>9754)
                     /locus_tag="PKI68_06035"
     CDS             complement(9257..>9754)
                     /locus_tag="PKI68_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX53285.1"
                     /translation="STVASMPGLLFILAIAMVVGKGLLGIYLGIGLTTWVGICRLIRG
                     EVMKHKEQAYVKAAHALGLSWPRILFRHILPNVSHVIIVTFTLRFPAAVSTEVIMSFL
                     GIGVASEPSWGVMINSARMRLWQGVWWEMTFVTIAIFGLVLAFNLLGDALRDALDPRL
                     RTEGN"
BASE COUNT         1956 a         2961 c         2966 g         1871 t
ORIGIN      
        1 aaagatggaa aggatttcag cctctatgcg agtcaacctg cttacagcct ccattaccgt
       61 catgatcacc gccggaacgg tgcaggccgt tgatctgctg accgcgtacc ggcaggcggc
      121 ggaatcgagt ccgctggtca aacaggccag cgcgcgctgg caggcggagc agtccggaaa
      181 agatatcgcc cgttccgcgc tcgcgccgcg gctgaccgtc gaaggcgccg ccagtcagaa
      241 ccagctcgac ctcaccggct tcggcgccgc gccgatcaac gaggaataca atccgtccag
      301 ttacagcgtg acgctgacgc aaccgctgat taacggtgcg gcctggtcca cgtttcaggc
      361 gtcgcgctcg aaggcgcaga gttatgaagc gggtctgctg gcggttaagc aggatttgat
      421 tctgcaaacc gcggacgcct atttcgccgt gctgcgcgcc gaagcgctgg agcggacggc
      481 gctcagtcag cagacgctgc tgcaaaaaat ctgcgaccgg gcggaaggcg aacgccgcgc
      541 cggcaccggc gatatgattg ccgttcagga agcccgcgcg cggctcgacg cggcgcaggc
      601 ggagctgctc agcgcccgca acggcgtaca gctggcgcgc cgcgcgctgg aaaaactgac
      661 gcatcagccc gtcgaaacgc tgaccgatct tgaaacgctg gaaccgcaag gtccggtgcc
      721 ggcgttgatg gaggagtggg tgaagtcggc ggaagtcagt cagccggtgc tggagcaggc
      781 gcggcaggaa ttgcaggcca gccgctatat gattgcggcg gcgtcccgcg gcaactggcc
      841 ggtggtttcg ctgaacgcgc agtatatccg cgcggacggt tcgtttatgc cggcgattga
      901 gcgccgcgaa agtctggtcg gcgtcagcgc cgtctggccg ctgtttgaag gcggccgggt
      961 gcgcgcttcc aaggcgcggg ccgaagcgct ggcgcgggtc agccagtacg ggctggaagc
     1021 gctgacggac aatgtgcggc tggagacgca gcgggctttt ctgaatctgg aaaacagcgt
     1081 ggcgcagctc accgcagcgc ggcgcgcgct ggagtcggcc caaacggcgc tgaaggcgac
     1141 gcagaaaggg tatgagatcg gcagccgctc ggtggtcgac gtactggaca gtgcgcggcg
     1201 gcaggccgcg gcggagtcgg actattatct cgcgctctac agtcaggtgc tttgccggat
     1261 gcagctcaaa gcctccgccg gtgtgctttc agaaaacgat gtgcatgcaa tcaaccaatt
     1321 gctgaccgtg aaaccgggag tgtaacgatg gacatgaaaa aagttctgcc gaaagcggtt
     1381 cccgcgctgc tgctgatcgc ggggatcggc ggcgttttga ttttcagaca tgaacaggag
     1441 atcagcaaac ggctgacgtt ttacggcaac gtcgatatcc gccagattca aacggccttc
     1501 tacagcaccg gccgcattca gcagatgctc gcccgcgaag gggacgccgt gacgaacggc
     1561 cagctcatcg ccgagctgga cgcgacccgc tacgaagcgg cggcggcgaa ggcggaagcg
     1621 caggtcgcca tgcagcagca ggtggtggcg cgcatgctgg ccggaagccg cccggaagaa
     1681 attgttcagg cgcaggcacg cgtccgcgaa gccgaggcgg cgctgaccga cgcgctgcaa
     1741 aaacaggtgc gggcgcaggc gctggcgaaa gaggaatatg tttcgcagca gcagctcgac
     1801 gacgcccgca cggcggtcga tacgaagcgc gccaatctgg aagcggtccg gcagtccgcc
     1861 gaactggcgg tgctgggtcc gcgcaaagaa gatatcgcgg cggcgcaggc gcagctcagc
     1921 gtttatgaag ccgcgctgaa gctggcgaag caggagctgg ccgacagcaa actctatgcg
     1981 ccggcggatg gcattattca gaaccgcatt ctcgaaccgg gcgatatggc gtcgccgcag
     2041 agtcccgttt atacgctggc gctgaacaat ccggtctggg tgcgcgccta tgtgccggaa
     2101 ccggacctcg gaaaaatttc cgccgggctg cgcgccgaag tgcgcaccga cagctttccg
     2161 ggcaaggtgt acgaaggctg ggtcggctac atttctcctt cgtcggagtt cacgcccaag
     2221 caggtggaaa ccaccgacct gcgcacgcgg ctggtttatc aggtgcggat ttatgtgaag
     2281 aatccgcaga acgaactgcg gctgggcatg ccggccacgg tgaaaattct caccggctcc
     2341 gcgcaggaga actagcgatg ccggttccgg ctgacagctc caccgttctc gaactccgcg
     2401 atgtcaccaa gcggttcgcg acctctgcgg cgttaaacgg cgtcagcctg agcgtccgca
     2461 gaggctccgt caccggactg atcgggccgg acggcgcggg caaaaccacg ctgatgcggc
     2521 tgatcgccgg gctgctggtt ccggaccgcg ggcggatttc cgtactcggc atcgacgccg
     2581 ttcaggagcc gctgaaggtt cagcatgtca tcggctatat gccgcagcgc ttcgggctct
     2641 acgaagattt gacggtcgaa gaaaatttaa atctctacgc cgatcttcag ggactgccgg
     2701 atgccgcgcg cccggcccgc tacgccgagc tgacgaagat gaccggactc gcgccgttca
     2761 cgaaacggct ggccggcaaa ctgtccggcg gcatgaagca gaagctgggg ctcgcctgcg
     2821 tgctggtgcg tccgccgcaa ctgctgctgc tcgacgaacc gaccgtcggc gtcgatccgg
     2881 tttcgcgccg agaactgtgg tcgattgttt accggctggt gcagcaggaa aaaatgacgg
     2941 cgctgctgag cacggcctat ctggatgaag cggagcgctg caatgaagtg attctgctgc
     3001 acaagggcga actgctcggc cagcagtcgc cggcggagtt cagccggcag gtcgacgggc
     3061 gcagttttca agccaccctg cccggcctga ataaacggtc tttgcaggga cggctggccc
     3121 gcgcccccgg cgttctggat gccacgatcc agcgcaccgg cgtgcggatt gtgatgaaaa
     3181 gcgcaacggc gccggacctc gccggactgc cgcacggaat ccggatcgaa ccggttccgg
     3241 cccgtttcga agacagcttc atcagcctgc tgcgcgaaaa ggacggacac agcgcgccgc
     3301 cggacagtcc gtcccaaccc ttcgcgccga ccggcggcga tatgattacg gtcgaacagg
     3361 tcacgaagcg gttcggttcg ttctgcgccg tgcgcggcat cagtttttcc gtgaaggaag
     3421 gcgaggtgtt cggcctgctc ggcgccaacg gcgcgggcaa gtccacaacg ttccggatgc
     3481 tgtgcggcct gctgccggcc acggacggaa aactgagcgt cgccggcctg gacctgcggc
     3541 acgctccggc caaagcccgc gcgcggatcg gctatatggc gcagaaattt tcgctctacg
     3601 gcgatctgtc ggtacaggaa aacctgcggt tcttcagcaa ggcgtacggc ctgcgcgacc
     3661 gtgaacagcg cgaacggatc gaatgggcgc tgcgggaatt cgagctgacg gactatgccg
     3721 cgagcaccag ccgcgaactg ccgctcggct ataaacagcg gctcgcgctg gccgccgcgc
     3781 tgatgcaccg gccgaaaatt atttttctcg atgagccgac ctccggcgtc gatccgctgg
     3841 cccgccgcga attctggcac cgcatctccg gcctggccga agaaggcgtc acagtgctgg
     3901 tcacgacgca cttcatggaa gaggcggaat actgcgaccg gctcgtgatt atggcggaag
     3961 gcgatattct ggccgagggc acgccggatg aactgcgcgc ccgcgtccgg tcggaacagc
     4021 tggcggaccc gacgatggaa gacgccttca tccatctgat cgaagaacac gaagcggcgg
     4081 ggaggcccga tgcggtttcg taaacagcct gatccggaaa agcgcgcggc acagatgcgg
     4141 ctgcgcggga tgctgcgcaa ggaagcgctg caaatcctgc gcgatccgtc gagtattgcg
     4201 atcgccttcg tgctgccgat cgtcctgctg ttcctcttcg gctacggcat ttcgctggat
     4261 gccaaacacg tgccgatggc ggtcgtcatc gaaagtccgt cggcggaagc tgcggatttc
     4321 gccgcgtcgt tcgagcagtc ggactatttt tcgccgcagc ggttcggcac cattcaggac
     4381 gcggaacagg cgctgatccg tcaccgggtg aacgggatcg tctggctgcg caatgatttt
     4441 gaagagaagc tgctgtccgg agcgtccgcg ccggtcggcg tgatcattaa cggcgtcgat
     4501 gccaacaccg cccgcattat cgaaggctat gtgaacggcg cgtggagcgc ctggctgcag
     4561 cagcgcgcaa ccgcgcaggc cgaaacgctg aagctgcccg tccggctgca gccgcgcgtc
     4621 tggttcaatg ccgaactgcg cagccgcaac ttcctgatcc ccggactgat cgcggtgatt
     4681 atgacgctca tcggcgcgct gctgacggcc atggtcgtgg cccgcgaatg ggagcgcggc
     4741 acgatggagg cgctgatggt tacgccggtg accgcgaagg aaattctgct cggcaaactg
     4801 cttccgtatt ttgttctcgg cctcggcggg ctggcgcttt ccgtcggcat ggcggtctgg
     4861 ctgttcggcg ttccgctgcg cggctcgctg ctggtgctgt ttctgacttc tgcgctgttc
     4921 ctgatcgccg cgctgggcat ggggctgacc atttcaaccg cggcccgcaa tcagtttgtc
     4981 gccggacaga tcgccatcat cgcaacgttt cttccggcgt tcatgctgtc gggatttatt
     5041 ttcgacatca acagcatgcc cgccgctatt cagatcgtca cccatctgct gccggcgcgc
     5101 tattacgtcg ccattctcca gaccgttttt ctcgcgggga atgtctggaa cgtcctgctc
     5161 ctcaattcgc tggcgctgat gctgttctgc gttttttttc tgggactggc ccggcggcgt
     5221 acggttaaac ggctggagta aaccatgtgg acgcacctca aagcactggt catgaaagag
     5281 ctgctggcgc tgctcaaaga caaaaagagc cggctggtgc tgattgcgcc gccgctgatc
     5341 cagcttttcg ttttcggcta cgccgccacg ttcgatctca accatatccg ctacgccgta
     5401 ctgaatgaag accgcggcgc ggcttcgcgc gaactgctcg cacgcttcga gggctccgga
     5461 accttcgagc gggtcgcgca actggacggc gatgcgcaga tcgagccggt catcgacaac
     5521 aaaagagcgc tgctcgtcgt acggtttccg tccgacttca gccgcaatct ggcacagggc
     5581 cggccggcag acgttcagat caacgtggat ggacggaatt ccaacagcgc catgctggcg
     5641 ctgaactacg tccagtccat cgtctcctca ttcaacgcgg tctggatgaa agaacacggc
     5701 ggccgggcgc cgcccgccaa cctcgtcgtc cgtccgtggt acaacccgaa tctggtcagc
     5761 cgctggttta tcgttcccgg catcatcggc ctgctgacac tgctcgtcac gctgctcatc
     5821 accgcgcttt ccgtagcacg ggaacgcgaa gagggaacct tcgatcagct gctggtgact
     5881 ccgctgaggc cttttgaaat tctgctgggc aaggcgattc ccggggtcct cctcggactg
     5941 gcggaagcct cggcgattct tctgattatc gtgttctggt ttaaaattcc gctgctcggc
     6001 agcctggcgg ctctttatct cggcctcctg ctcttcctgc tttcctccat tggcgtcggc
     6061 ctgatgatct cctcgctgac ggtcacccag cagcagggcc tgctcggcgc gtttctcttt
     6121 ctggttccgt ccatcatcct gtccggattt tcaacgccca tcgccaatat gccgctctac
     6181 ctgcagaaac tgacactgat caatccgctg agatacttca tcgtcatcct gcgcagcgtc
     6241 tttctggaag gcgcgaccgt gcctttgctc tggccgcagt actggccgat ggcgctcatc
     6301 gggtgcatct gcctttttat ggccgccatc cttttccgac gccgcatggt ttgaaatcag
     6361 cagaaattgc gcagagcaat catgttggtc tgcttccatc cctgtttgcg gtagaaaata
     6421 agcgccggaa cattgcgccc gtccgccagc agctgaacgc gcgttgcgcc ctgttcgcga
     6481 gcccatttca ccgcggcagc cagcaaggct ttaccaagtc cgcgccgacg gcagatctgc
     6541 gaaacaacaa gatcctcaat cagtccggaa aatccgccgg ttgcggttga gatcaccatc
     6601 tgtacggtca ccatgccggc aacccgcccg tgccgttcag ccacccagac cgatgcggat
     6661 gcggattcca gcagcatctg gagaccgcac cgttgtttga ccgcatcaaa ttcgaattcc
     6721 gattctatcg tgaaaagctc ctgcagcagc cgcatcatag ccgcaagatc gtcagtgccg
     6781 gcaggccgta tccagaacgc actcttttca gttcccgatt tcactcctat taccggctcc
     6841 atagacccct cacatctttg tcccgaaaac acccggataa gttcacaact gtaacattcc
     6901 ggccttcgcg caggcttggt ttgaattaaa aagcaacttc ggtgccgaac cggcgcaggg
     6961 atatacccaa aacacaaaaa ccgctgataa ataaggttaa tcatttgtcg cacttgacat
     7021 ttccggcgta tccggcacca tccgagccat caaattaaag cacttccgac cggtcttgcc
     7081 gggcgagtgt ccgtcccgcg ttagaagtgc ttgttggagt tggttgttta agtgaggtgt
     7141 atcatgagtt ggatgaaaaa tgttttgtcc attaccgttc tttctttact ggtcgtcgcg
     7201 acgggttgcc gttcgccgaa aatgccgttg acccccgcac agcagggtgc tgttgttggc
     7261 ggttcaggcg gtgcagttgt tggtgccgtc atggctcaaa atgccaatgc ttctctcagc
     7321 agctggcagg gagctggcgc gggtgcagta gctggcgtgg ccgccggcgc actggttggc
     7381 gatgctctcg atgaatacgc ctacgacaac gcttccggca tgaaagacaa agatgctcag
     7441 atcaatgatc ttcagcgtca gctcgcagat gccgaagcca aactccgcgg tcagggcatg
     7501 gacaacgtga aggttgaagc cgttaacggc cagctccgct tcaccatcct gaacgaagta
     7561 ctcttcgaat ccggaaaagc tgaactgaaa accggcagca aatcgaccat cgacagcgtg
     7621 ctgaatatca tccagagcga tttcgctaac cgcgacatca ccatcgaagg tcacactgac
     7681 agcgatccga tcatgaagtc gaagtggaaa gacaactggg aactttccta caaccgcagc
     7741 atggcggttc tgtactacct catgaacgaa aaaggcattc cggctgagcg tctcaaagca
     7801 gttgcctgcg ccgattcaca gcccgttgcc ccgaatgaca gcgcagccaa caagcggctg
     7861 aaccgtcgtg cggtcatcgt tgtgatgccg gccaaggact ccatcatcgt cgagaagaag
     7921 taagtatcct tgcgtagacc ttctcacatg aaatccgccg ttccattgtt tatcactgga
     7981 acggcggttt tgtttttact gtgcggctgc gccacatcgg tcgaaagccc gatccgcaaa
     8041 ccgcaaccct gtgccgcgcc gcagcctctg cgggtggccg tcctgccgcc gcgcattcac
     8101 ccggatcttc tgccggaaaa cctcaaatcg gaaaagccgc tgtataaaca gatgatggat
     8161 ggccgcgggg cggtcgtcac cagcgggtca gcatccaaag acatcggcct ggaaatcgtc
     8221 tccaccctga cacaggccgg aaaatacggg cggatattca ccgttgaaac tgaagaggaa
     8281 gcccggcagt tgggcgccga tgccctgctg accgttaccg tctgggatta ccgcaccgta
     8341 cagctcggct ccaataaaaa gtatccgctg attgtggcgc tgggcctgct catgccgcaa
     8401 tactggatac gctggcagac gattgaggcg cgcttcgaat gggaggttaa actcacctcc
     8461 ctcgccgacg cttccacact ctaccgcaac cggctcaaac gcgaatacac atcttctgtc
     8521 cgctcggcct ccggccatta cttcaccgac aaaatgcttt cattcctgca gaaccgcgcg
     8581 gcgcctgact tcatcggcga actgttcgcc ttggagatgc tgaccccgcc ggctgaaaag
     8641 tagatttaaa cgttccgaca gacccacctg cgagaccgag tcatgaaacc attctgtgcc
     8701 ctttttctgg ctgtgaatat tggattgatc gcatgcgggg cgggtgccga cacgaaccga
     8761 ccgccctcct gggcaactcc gattgaactg gccggcgttc ccaatcttca caaggtgagt
     8821 gatacgctct atcgcagtgc gcagccgacg gccgagggca tgaagaacct ggaaaagatg
     8881 ggcattaaaa ccgtgatcaa ccttcgttct tttcattcag accgggacga actcaggggc
     8941 accagcctgt ccggcgaaca tcttttcatg aaggcctggc acccggaaca aaaagaggcg
     9001 gaacggtttc tgcagattgt gaccgacccg gcacgcacgc cggtactggt tcactgccag
     9061 cacggagcgg atcgcaccgg aactctttgc gccatctacc ggattgcggt tcaaaactgg
     9121 cccaaggatg ccgcgatccg cgaaatgacc ggcggcggat tcggcttcca tgaaatatgg
     9181 agcaacctgc cggcctggat cgagcagctg aacatcaatc agctcaactg caaaccgccc
     9241 gttcaatagg cgcagactaa tttccttcgg tgcggaggcg cggatcgagg gcgtcgcgca
     9301 gagcgtcgcc gagcaggttg aaggcgagga ccaggccgaa gatggcgatg gtgacaaagg
     9361 tcatttccca ccagacgccc tgccagaggc gcatgcgcgc gctgttgatc atgacgcccc
     9421 acgacggctc gctcgcaacg ccgataccga ggaagctcat aatcacctcc gtgctgacgg
     9481 ccgccggaaa gcgcagggtg aaggtgacga tgatgacgtg gctgacattg ggaaggatgt
     9541 ggcggaaaag gatgcgcggc cagctcagtc ccaatgcgtg cgccgccttc acgtaggcct
     9601 gttctttatg cttcatcact tcgccgcgga tcaggcggca tattccgacc caggtcgtga
     9661 gtccaatgcc gaggtaaatg cccagcagcc ccttcccgac caccatggcg atggcgagaa
     9721 tgaagagcag gcccggcatg gaagcgacgg tgga
//
LOCUS       DAOPZO010000074         9753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_8183_length_9753_cov_8.729016, whole genome shotgun sequence.
ACCESSION   DAOPZO010000074 DAOPZO010000000
VERSION     DAOPZO010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 9753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9753
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8183_length_9753_cov_8.729016"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /gene="purH"
                     /locus_tag="PKI68_06040"
     CDS             complement(<1..794)
                     /gene="purH"
                     /locus_tag="PKI68_06040"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNX53286.1"
                     /translation="MTKITRALLSVSDKAGIVDFAKALVARGVELLSTGGTAKALRDA
                     GIAVKDVSEFTGFPEMLDGRVKTLTPQVHAGLLHLRDSEEHMATMKAHGLQPIDLVCV
                     NLYPFEATIAKAGVPLHEAIEQIDIGGPTMIRSAAKNMKFVTVITDPSDYARVLESMD
                     ANGGNTTEELRFELGQKVFARTAAYDATIAGYLAEQMKTVVPRPFVKTAGNGVELRYG
                     ENAHQKAWLYTNPLSDEACIAHTDVLHGKEMSYNNYVDGEGALEAV"
     gene            complement(1077..1331)
                     /locus_tag="PKI68_06045"
     CDS             complement(1077..1331)
                     /locus_tag="PKI68_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53287.1"
                     /translation="MNHQATSRFWDCYEQLPGAVRDLADTSFDLLKENPRHPSLHLKK
                     VNQLWSVRVGRKYRALAVQQEDTLIWFWIGTHAEYDRLIG"
     gene            complement(1328..1528)
                     /locus_tag="PKI68_06050"
     CDS             complement(1328..1528)
                     /locus_tag="PKI68_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53288.1"
                     /translation="MTTVKEIEKAVEHLPPNDLSQFRRWFEAFESASWDRQFEADARN
                     GRLESLVNEARAEYKTGKTSSL"
     gene            complement(1716..2900)
                     /locus_tag="PKI68_06055"
     CDS             complement(1716..2900)
                     /locus_tag="PKI68_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNX53289.1"
                     /translation="MKTNDPKSLHNRIAAHVRDIPRSGIREFFDIVSTRSGVISLGVG
                     EPDFATPWHIREAAITALEQGVTSYTSNFGLLALRKEIAGYVGKLTGVTYNPENEILI
                     TVGVSEAMDLAIRALIEPGDEVLYHEPCYVSYNPVVVFAHGVPVKVETSVENGFKLTR
                     KQLEAKVTKKTKLLLLNFPTNPTGAVLTRQEVEEIAAFAIEHDLIVLTDEIYGELTYD
                     QPHVSLLSVPGMKERTVYLHGFSKAWAMTGFRMGFSCAPPELTEAMMKIHQYTMLCAP
                     VLSQKAAIEALRDPARDIDEMRDSYCVRRNYMHAAFQEMGVNCHLPGGAFYMFPDIRQ
                     FGLSSRDFALRLLEEENVAVVPGTAFGACGEGSVRCCYATALEDIKEAMTRIARFVKK
                     LG"
     gene            complement(2869..3348)
                     /locus_tag="PKI68_06060"
     CDS             complement(2869..3348)
                     /locus_tag="PKI68_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNX53290.1"
                     /translation="MDELLKQLKNNAKESPENLAKLLGTTAADIKARIDAYEKAGIIQ
                     GYQAVIDEDKLDGAPVTAVIEVRLTAEREGGFNRIAERISRFPEVRSAFLMSGGFDLL
                     LFVSGKTLNEVASFVSEKLSPLGGVVGTATHFMLKAYKKNDVLMRGSDENERPQISA"
     gene            complement(3456..4214)
                     /locus_tag="PKI68_06065"
     CDS             complement(3456..4214)
                     /locus_tag="PKI68_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53291.1"
                     /translation="MKKRVIMIMVAALAAGVARAADVSATADFASAYIFRGVTLNDGL
                     VFQPGAKISGFPIPEEYGSVAVGTWANYDINDFGGRLNKNEFSEIDYYLSYTLPVKIV
                     DISATYTEYTYPNGGGTEADKEIALSVGKAIGETGLYPSITANYGLGGAVENNWYIQG
                     GLDYTKALTDALTFSSGVKIAYLISDTGKDGFNDATAKVGLSYALTKNWAINGSLNYV
                     ARLDDDVLADADYSADTLGYDKDFFATLGLSCSF"
     gene            complement(4608..5042)
                     /locus_tag="PKI68_06070"
     CDS             complement(4608..5042)
                     /locus_tag="PKI68_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNX53292.1"
                     /translation="MGRVKLDMPSVYPFKTELTVRITDINYGGHLGNADVLALMHEAR
                     VRFLKSFGYSEVSVEGYGTIMLDSVVIYKSQAFAGDVLQIEIAAGDFSRIGCDIFYRI
                     TKKETAAEVALAKTGIAVFDYAGQKRVSPPEAFVRKLGGRLS"
     gene            5151..5315
                     /locus_tag="PKI68_06075"
     CDS             5151..5315
                     /locus_tag="PKI68_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53293.1"
                     /translation="MRKKPLRRPTKKAGPKRYRVAQQKKRLVAVGIPEAAIRRMTNKD
                     IREALMKNNA"
     gene            5387..5905
                     /locus_tag="PKI68_06080"
     CDS             5387..5905
                     /locus_tag="PKI68_06080"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNX53294.1"
                     /translation="MRDKNIILIGFMGTGKTVTGRVLAERTGMKLIDMDSLIETEQKK
                     TIPEIFAQDGEPAFRQMERELVQKLSKRSGLIISTGGGIVLNPDNVADFEKSGLVVCL
                     TASPETIFKRVEKDTSRPLLSGDKKTQIYTLLEKRKPLYEAISHRINTDGLTDKQTAN
                     RILELYALESRD"
     gene            5945..6595
                     /locus_tag="PKI68_06085"
     CDS             5945..6595
                     /locus_tag="PKI68_06085"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX53295.1"
                     /translation="MMDTQNDGLQKKHELEKHEVQEVLGFLKRYGKLIGAGILAAAVA
                     VIVSRGFAHHKASQIAEAERLLLEAQAPQQLENIVTKYSSTPTAPVALLNLAKTYFNN
                     GDYVQARAQYDRFLKKYKNHEMSPVAELGLAYCTEADGDFAGAAAQFAAFAEKHSKNY
                     LQPLAVLSIARCKEQAGQLDQARIVLEDFLAQNAGSPWAGSAEGALKSLNETAQKK"
     gene            complement(6597..7289)
                     /locus_tag="PKI68_06090"
     CDS             complement(6597..7289)
                     /locus_tag="PKI68_06090"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HNX53296.1"
                     /translation="MSAQKTYTIRCPKCGHAQKVELYDSINVAQQPELKTALFENRLN
                     RVLCESCEASFRIDKPLLYHDADRNILIHWMPDTAVSREEILDEFDRSMDELRKALPE
                     DMEPPRVRLVFTRAELVELVFIMEAGMEERVVEYIKYTIHTQNMNRVPPADKHLLLNV
                     QDSTAEELLFAVQNAATFELEEVLRYPRAAYQSVREMYRKNPDEFTELFPGPYISARA
                     ALMDENRENDEE"
     gene            complement(7362..7874)
                     /locus_tag="PKI68_06095"
     CDS             complement(7362..7874)
                     /locus_tag="PKI68_06095"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNX53297.1"
                     /translation="METIQTIMTRRSIRAFDETPVTEAQLKTLLEAAMNAPSAGDGRP
                     WHFVVTTNRAQLDALADALDEGRPMFKQAQAAILICLDESLEKFKGFGPQDCACAAQN
                     LHLAAHDLGLGSVWVAVIGVPPRVAGCRKVLGVPENLMPFALFPIGVPGEKLDPEYRY
                     DESKIHRERW"
     gene            complement(8063..8995)
                     /locus_tag="PKI68_06100"
     CDS             complement(8063..8995)
                     /locus_tag="PKI68_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HNX53298.1"
                     /translation="MKNLLKIRHYSPRTLQTYTEWAQRYFGYAKRQKLAWDAPETIRV
                     WLSSLATQHNVAASTQNQAFSAVLFLFREVLKADLPEVNAVRAKRGPKLPVVLTPEEV
                     QKVFSCSEGTVGLMLRLSYGAGLRISELIRLRVQDIDFGNETLLVRSGKGDKDRATTL
                     PASLTSELKAHLDRVRALHEADLAKGFGAVWLPGALARKYPNAPTEWKWQYVFPAKNL
                     SVDPEGGMTRRHHVSEQILQRALKKAADAADIPKRVSIHTLRHSFATHLLMQGVNIRE
                     VQELLGHKSVETTMIYTHVVRTMSNRPKSPLDTL"
BASE COUNT         2209 a         2753 c         2582 g         2209 t
ORIGIN      
        1 ttgacggctt cgagcgcgcc ttcgccgtcg acatagttgt tgtagctcat ctctttgccg
       61 tgcagcacat cggtatgcgc aatacaggct tcgtccgaca gcggattggt gtagagccag
      121 gctttctggt gggcgttttc gccgtagcgc agttcgacgc cgttgccggc ggttttaacg
      181 aacgggcgcg gcacaaccgt cttcatctgc tccgcgagat aaccggcgat ggtcgcgtcg
      241 taggccgctg tgcgggcaaa tactttctgg ccgagttcga aacggagctc ttcggtggta
      301 ttgccgccgt tagcgtccat ggattcaaga acgcgggcgt agtcggacgg atcggtgata
      361 acggtgacga acttcatgtt tttggcggcg gagcggatca tcgtcggccc gccgatatcg
      421 atctgctcaa tcgcctcgtg cagcggcacg ccggctttgg cgatggtggc ttcgaacgga
      481 tagaggttta cgcaaacgag gtcgatcggc tgcaggccgt gagccttcat agtggccata
      541 tgctcttcgc tgtcgcgcag atgaagcagt ccggcgtgaa cctgcggagt cagcgtcttc
      601 acgcggccgt cgagcatttc cggaaagccg gtgaactcgg aaacatcttt gacggcaata
      661 cctgcatcgc gcagcgcctt ggcggttccg ccggtcgaaa gcagttcgac gccgcgggca
      721 accagcgcct tggcaaaatc aacaattccg gccttgtctg agacgctcaa cagcgcccgt
      781 gtaatttttg tcattaaata ctccttagga aaacggttag gctcggattt aagcatgaac
      841 cgcggagccg acaaaccaat tttccaaggt ttggaaactt ttcttccaag gtctggaatc
      901 cagctgcgcc gagagcttct ttttgcctcg cagagattcg gaggacgcag agaaaaacct
      961 aaaatcgctc ggctttctct gcgcctctgc gaggaatctc tttccaatgt ttggaaaccg
     1021 gcggtcagcc gagctttttg acgaagcggg cgatgcgggt catggcttct ttgatgtcag
     1081 ccgatcagtc tgtcgtattc agcgtgagtt ccaatccaga accagatcaa ggtatcttct
     1141 tgctgcacag ccagagcccg atactttctg ccgacgcgca ccgaccagag ctgattcact
     1201 tttttgagat gcagcgacgg atgccgggga ttttctttaa gcaaatcgaa ggaggtatca
     1261 gccagatcgc gcaccgcccc cggaagctgc tcgtagcagt cccagaaacg ggatgtggct
     1321 tggtgattca cagcgacgag gtcttccccg ttttgtattc ggcgcgagcc tcgttaacca
     1381 gcgactccag acgtccgttg cgggcatccg cctcaaactg ccgatcccat gacgcagatt
     1441 cgaacgcttc aaaccatctg cgaaactggc tcaaatcgtt gggcggaaga tgttcaaccg
     1501 ccttttcaat ttcttttacc gttgtcatgc ccgctttata ggccctgacc ttgttttttt
     1561 caaggcgaat gaaaaatccg tccgggcaga cggctaccgg caccaaaatc tttttgcctc
     1621 gcagagattc ggaggacgca gagaaaaacc taaaatcgcg cggctttctc tgcgcctctg
     1681 cgaggaatct ctttccaatg tttggaaacc ggcggtcagc cgagcttttt gacgaagcgg
     1741 gcgatgcggg tcatggcttc tttgatgtct tcgagggcgg tggcatagca gcagcggacg
     1801 ctgccttcgc cgcaggcgcc gaaggcggtg ccgggtacga cggcgacgtt ttcctcttcg
     1861 agcagccgga gcgcaaagtc gcgcgagctg agaccgaact gccggatatc cggaaacatg
     1921 tagaaggccc cgccgggcag atggcagttg acgcccattt cctgaaaggc ggcgtgcatg
     1981 tagttgcggc ggacgcaata gctgtcgcgc atttcgtcaa tatcgcgggc cggatcgcgc
     2041 agggcttcga tggcggcttt ctgactgagt accggagcgc aaagcatggt gtactgatga
     2101 attttcatca tggcttcagt gagttccggc ggcgcgcagg aaaagcccat gcggaagccg
     2161 gtcatggccc aggctttgga gaagccatgc agatagacgg tgcgctcttt catgccgggg
     2221 acggagagca ggctgacgtg cggctggtcg taggtgagtt cgccgtaaat ttcgtcggtc
     2281 agcacgatca gatcgtgctc gatggcaaag gcggcaatct cttcgacttc ctgtcgcgta
     2341 agaaccgctc cggtcggatt ggtcggaaag ttgagcagca gcagtttggt ttttttggtg
     2401 actttggctt cgagctgttt gcgcgtcagt ttaaatccgt tttcgaccga ggtttcaact
     2461 ttgaccggca cgccgtgggc aaaaaccacg accggattgt aggaaacata acacggctcg
     2521 tgatagagca cttcgtcgcc gggttcgatc agcgcgcgga tggccagatc catcgcttcg
     2581 ctgacgccga cggtgatgag aatttcgttt tccgggttgt aggtgacgcc ggtgagcttg
     2641 ccgacatagc cggcaatctc tttgcgcagc gcgagcagtc cgaagttcga ggtatagctg
     2701 gtgacgccct gttcaagcgc ggtgatggcc gcctcgcgga tgtgccacgg cgtggcaaag
     2761 tcgggctcgc ccacgcccag gctgataacg ccagagcggg tgctgacgat gtcaaaaaat
     2821 tcgcggatgc cggagcgcgg aatatcgcgc acatgggcgg caatgcggtt atgcagagat
     2881 ttggggtcgt tcgttttcat cggaaccacg catgaggaca tcgtttttct tgtaggcttt
     2941 gagcatgaaa tgggtcgccg tgccgacgac gccgcccagc ggcgagagct tttcgctgac
     3001 gaagctggcg acttcgttga gggttttgcc gctgacaaac agcagcaggt cgaacccgcc
     3061 ggacatgagg aaggcggaac ggacttcggg gaagcggctg atgcgttcgg cgatgcggtt
     3121 gaagccgcct tcgcgttcgg cggtcagacg gacttcgatg accgccgtaa ccggcgcgcc
     3181 gtcgagcttg tcttcgtcaa taacggcctg atagccctga ataatgcctg ctttttcgta
     3241 ggcatcgatc cgcgctttaa tgtcagccgc ggtagtgccc agcagtttag ccagattctc
     3301 cggactttct ttcgcgttat ttttaagctg tttgagcagt tcgtccattt tcttctcccg
     3361 ttggaaagcc tgctttataa acggataacc cttggaaaca aacaaaaaag cgccccggat
     3421 atccggggcg ctttctgcta actgctttct gcgcgctaga agctgcagga cagaccgagg
     3481 gtggcaaaga agtccttgtc gtaaccaagc gtatctgcgg aatagtccgc gtctgccagc
     3541 acatcatcat ccagccgggc aacatagttc agcgaaccgt tgatcgccca gtttttcgtc
     3601 agtgcatagc tcagtccgac tttagcggtt gcatcgttga agccgtcttt tccggtatca
     3661 ctgatcaaat aggcgatctt cacaccggaa gagaacgtca acgcgtcagt cagcgctttg
     3721 gtgtaatcaa gtccgccctg aatataccag ttgttttcca ctgcaccgcc cagaccatag
     3781 ttggccgtaa tagaaggata aaggccggtt tcgccgatcg ctttaccgac actcagtgca
     3841 atttctttat ccgcctctgt cccgccgccg ttgggatagg tgtattctgt ataggttgcg
     3901 ctgatatcaa cgatcttcac cggcagtgtg taggacagat agtagtcgat ttcggaaaac
     3961 tcattcttgt tcagccgtcc gccgaaatcg ttaatatcat aatttgccca ggtgccgacc
     4021 gccacggaac cgtattcttc cggaatcgga aatccggaaa ttttcgcgcc gggctgaaag
     4081 accagtccgt cgttcagcgt gacaccgcga aagatgtagg cgctggcaaa atccgccgtg
     4141 gcgcttacgt ccgccgcacg ggcgactcct gcggccagag ccgcaaccat tatcattatt
     4201 actctttttt tcattgctcc tgtttctccc ttttttcctg tttcaatttc gaatgcactc
     4261 tgtgaaccct ttccccgtcc agacttcaaa tgattcacca gatgttttgg acagagcctg
     4321 tatcctccgc aacttcctgg tgcagattgc gaacatgctc tgcatttagc aaaatacatg
     4381 ccttaagatg ttttgatttg taatggtctg acggttaagc gcttacaaat tataggtata
     4441 aagacaatat tggtttaaac gatttcagaa cgactatttt gtatcatttg atacaaagaa
     4501 aacctttttg tatcaaaacg gtcgttcgtg ccggttcaga ggaccgggcg gcagcatctg
     4561 cgggtacagc aggaccggca gaatgagtag aattatttca gggccgacta tgaaagccgg
     4621 ccgccaagct ttcgaacaaa cgcttcgggc ggtgaaacgc gcttttggcc ggcataatca
     4681 aaaaccgcga tgccggtctt ggccagcgcg acttctgccg cggtttcttt tttagtaatg
     4741 cggtaaaaaa tgtcgcaacc gattcggctg aagtcgccgg ccgctatttc aatctgcaga
     4801 acatccccgg cgaaggcctg cgacttatag ataacaaccg aatccagcat gatggttcca
     4861 tagccctcaa cagagacctc gctataaccg aaagatttca aaaaccgtac gcgggcttcg
     4921 tgcattaaag ccagaacatc ggcgtttcca aggtgcccgc cgtaattgat atccgtgatc
     4981 cggacagtca gctcggtttt aaagggatag acagacggca tatcgagttt tacacggccc
     5041 acggaaaacc tcctttttta ggttgattca ctcaaccggc ctgtgtagct tacaccgctc
     5101 gttttaaaac gataagcctt tggagaagaa aaatgagaat tcgccgttca atgcgtaaga
     5161 aaccgcttcg ccgtccgaca aaaaaagccg gacctaagcg ctaccgtgtg gcccagcaga
     5221 aaaagcgtct ggtcgccgtc ggtatcccgg aagctgccat tcgccggatg accaacaaag
     5281 acatccgcga agcgctgatg aaaaataatg cctaagctgt atttacaggt ttgtttttgt
     5341 atagtgcgtc ccgaaatttc gggacgcatt ttttattaga ggatccatgc gagacaaaaa
     5401 catcattctg atcggattca tgggcaccgg caaaacagtg accgggcggg tgctggccga
     5461 acgcaccggc atgaaactga ttgatatgga ttccctgatc gagaccgaac agaagaaaac
     5521 catccctgaa atctttgcgc aggacggcga accggccttc cggcagatgg aacgcgaact
     5581 ggtccagaaa ctttccaaac gcagcggcct cattatttca accggcggcg gaattgtact
     5641 gaatccggac aacgtcgccg attttgaaaa gagcggactg gtcgtctgcc tgaccgcttc
     5701 gccggaaacc attttcaaaa gagtcgaaaa agacaccagc cggcctcttc tttccggcga
     5761 caaaaaaaca cagatttaca cgctgctgga aaagcgcaaa ccgctctatg aagccatctc
     5821 acaccgcatt aataccgatg gtctgaccga caaacagaca gccaaccgga ttctggagct
     5881 ctacgcgctt gaatcccgag actgaaaaga taagagtacc cgccttcaaa acaggagttt
     5941 agttatgatg gatacgcaaa atgacggtct gcagaaaaag catgaacttg aaaaacatga
     6001 ggtgcaggaa gtcctcgggt tcctcaaacg ctacggcaaa ctgatcggcg ccggcattct
     6061 cgcggccgcc gtagccgtaa tcgtatccag aggtttcgcc caccacaaag cctctcagat
     6121 cgccgaagca gaacggttgc tgctggaagc tcaggctccg cagcagctgg aaaatatcgt
     6181 aactaaatac agctcgacgc cgaccgcacc ggttgccctg ctcaatctgg ctaaaaccta
     6241 tttcaacaac ggcgactacg ttcaggcccg cgcgcaatac gaccgcttcc tgaaaaaata
     6301 taaaaaccac gagatgagcc ccgtcgccga actggggctg gcctattgca ctgaggccga
     6361 cggcgatttc gccggcgcgg ccgcacagtt tgctgcattc gccgaaaagc acagcaaaaa
     6421 ctatctccag ccgctggccg tcttaagcat tgcccgctgc aaagaacagg ccggacagct
     6481 tgaccaggcc cgcatcgtac tcgaggattt cctggcgcaa aacgccggtt cgccgtgggc
     6541 cggatcagca gaaggcgcgc tgaagtcgct caacgaaacg gctcagaaga aataaatcac
     6601 tcttcgtcgt tctctctgtt ttcatccatc agcgccgcgc gggcgctgat gtaagggccg
     6661 gggaaaagtt ctgtaaattc atccggattc ttccggtaca tttcgcggac actttgatag
     6721 gccgcgcgcg gataacgcag aacctcttcc agctcaaacg tcgccgcatt ctgtacagcg
     6781 aagagcagct cctcggccgt tgaatcctgc acattgagca gcaggtgttt gtccgcgggc
     6841 ggcacccggt tcatattctg cgtgtgaatg gtgtacttga tgtactccac cacgcgctct
     6901 tccatgcccg cttccataat gaacaccagt tcgaccagct cggcgcgggt aaagaccagc
     6961 cgcacacgcg gcggttccat atcctccggc agcgccttgc ggagctcgtc catgctccgg
     7021 tcgaactcat ccagaatctc ctcgcgcgac accgcggtgt ccggcatcca gtgaatcaga
     7081 atattgcggt cggcatcgtg atagagcagc ggcttgtcga tccggaacga cgcctcgcac
     7141 gattcgcaca gaacgcgatt gagccggttt tcgaacagcg cggttttcag ctccggctgc
     7201 tgggccacat tgatggagtc atacagctcc actttctgcg catgcccgca cttcgggcag
     7261 cggatcgtat aggttttttg tgcactcact acaaactcca gatcgtatta cgtactacct
     7321 actgcgtatt gagcaatacg gaatacgcaa tactcgttac actaccatct ctcccggtga
     7381 atcttcgact catcataccg atactccgga tcgagctttt ctcccggcac accgatcgga
     7441 aacagcgcaa acggcatcag attttccgga acgcccagta ccttgcggca gcccgccacg
     7501 cgcggcggca cgccgatcac ggcgacccag accgagccca gccccagatc gtgcgccgcc
     7561 agatgcagat tctgcgccgc acaggcgcag tcctgcgggc cgaagccctt gaacttttcg
     7621 agcgactcgt cgaggcagat cagaatcgcg gcctgcgcct gcttgaacat cggacggcct
     7681 tcgtccagcg catcggccag cgcgtcgagc tgcgcgcggt tcgtcgtcac cacaaaatgc
     7741 cacggacgtc cgtccccggc gctcggcgca ttcatcgccg cttccagcag cgttttaagc
     7801 tgcgcttccg tcaccggcgt ttcatcaaac gcacggatgc tccggcgggt cataatggtc
     7861 tgaatcgttt ccataaaatt ttcccctcaa aatcaggcgg catcttgccg gacgaaatca
     7921 tttcccgcaa gtgaacagcg gttttcaatg gttggaaaaa tcgccttcag gtttcaggct
     7981 ctggaaaaag gttgccgatc atttccaagg gttggaagtc attccatgac ggattgctgt
     8041 ttttttccaa gatattggaa cgctaaagcg tatcgagagg gcttttgggc cgattgctca
     8101 tggtgcgcac cacgtgggta tagatcatcg tggtttccac gcttttatgg ccgagcagct
     8161 cctgcacctc gcgaatgttt acgccctgca tcagcaaatg ggtcgcaaag ctgtgacgca
     8221 gcgtatggat tgatacccgc ttgggaatgt cggcggcatc cgccgctttc ttgagcgccc
     8281 gctgaagaat ctgttcgctg acgtgatgcc ggcgggtcat cccgccctcc gggtccaccg
     8341 acagattctt cgccggaaaa acatattgcc atttccactc cgtcggagca ttcggatatt
     8401 ttcgggccag cgcgccgggc agccaaaccg caccaaaacc ttttgccaaa tcggcttcat
     8461 gcaaggcccg cacccggtcc agatgagctt tcaactcgct ggtcaggctg gccggaagcg
     8521 tcgttgcccg gtccttatcg cccttgccgg aacggaccag cagggtttcg ttgccgaaat
     8581 caatatcctg gacacgcaac cggatcagtt cggaaatcct cagccccgcg ccatagctca
     8641 gccgcagcat caggccgacc gttccttccg aacaggaaaa aaccttctgc acctcctcgg
     8701 gtgtcagcac caccggcagc ttcgggccgc gcttggcgcg aaccgcattc acctccggca
     8761 gatcggcttt aagaacctcc cggaaaagga aaagaaccgc actgaacgcc tgattctgcg
     8821 tcgaagcggc cacgttgtgt tgcgttgcca gcgacgaaag ccacacgcga atggtctccg
     8881 gcgcgtccca cgccagcttc tgtctttttg catacccgaa ataacgctga gcccactccg
     8941 tataggtttg cagcgtgcgc ggcgaatagt ggcggatctt cagcagattc ttcatctcgc
     9001 gcagcgccat ctgctctgca ctgatcgtag acgcgccgcc ctcgtcgcgt tccgttttgg
     9061 agggtcgcgg gcctccgcga ccggacggcg aggccgccgt ccctccagaa acgccctcgt
     9121 cacgttttcc aatgactgga aagtctgatc tctggcctct gacctccgac ctctgatcgg
     9181 ttccgccctc tgcgccatgc tcaataccgt tattcttcac gggcagaaaa acgtttagat
     9241 acagctccac agccttcacg gcctgccgac gctgccagtc ttcaagagaa tcatcgcgag
     9301 ccaattgatc cgaaaagcat tgtaaaagat cccctgcggt gaggtgctct cgatcgaact
     9361 ccgccctcag gaagcgaaaa atccatttca ggtaaaaggg aagatgccgc tcatccacca
     9421 gattccggct ccgcacaaaa tcctcaaatg gttgcaaatc aatatattgc atttgaatca
     9481 ccccttatta cctttttatg catcatgatg cataaaaagg taataaagac aaaaaccatc
     9541 gctttcaagc cggatttccg ccgttttgat aatgatcaat atttttatgc atcatcactc
     9601 cgccccgctc aagcaaacga ataggtccca aaaacaagca cttctttaca ttaaaaaaca
     9661 aaattaaagg tctttgactc gcactcaaaa agaatccaag cttttcgcaa ctcagcgtcc
     9721 ggcaattccg tcatgacgca gaaacaacgt tcg
//
LOCUS       DAOPZO010000075         9677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_8279_length_9677_cov_8.626689, whole genome shotgun sequence.
ACCESSION   DAOPZO010000075 DAOPZO010000000
VERSION     DAOPZO010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 9677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9677
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8279_length_9677_cov_8.626689"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1079)
                     /locus_tag="PKI68_06105"
     CDS             complement(<1..>1079)
                     /locus_tag="PKI68_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53299.1"
                     /translation="GLGGAGCNTIAHIAPRAPEGMEFAVMDCDIQTMETCSNIENRIA
                     AGKALTDGMSTGGDLEVGRRCAENSAGQIETMLSGVDLLMVITGLGGGFGTGAAPVIA
                     RMARHLGTTTLFFTVLPFPFEGLTAQGRAHSAIRRLRTYADAIVQMPNALIQPEGNAI
                     LGDSLERSSRTLAAGVIGIWRMLAYTGICNLDFATLQTMLSYCDAFCRFSCATATGEG
                     RTDALIDSLRTHPLVKDTAVFEKAPGMIIGITGGEDLKLSEIQQIVETIAPDKEECWI
                     KLGIAIDPAFTGRISAIVLAAEAWKEPLVDDGRGGLKPVSGDRKQGELAGVLKPRSRT
                     FGGAERTIWEGEDLDVPTYIRRKIKL"
     assembly_gap    1080..1179
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1180..2396)
                     /gene="ftsA"
                     /locus_tag="PKI68_06110"
     CDS             complement(<1180..2396)
                     /gene="ftsA"
                     /locus_tag="PKI68_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNX53300.1"
                     /translation="MAAPFITALEIGTTTIKVLMAEVREDGGLMIAGLGECESRGVRK
                     GEIVDFELALDAVRRAMEAAEAVAHKSIGESVYLVASGGRAESLLHVGGIPVLNEYEE
                     PGGEITREDIDQALEVARKIPLPDDRIRLHTLQQHFEVDGRGGIVNPERMLAEELRVG
                     MLLIHGQRAVVENLKKLTENVPVVCADAAFGGFCSAQAVLAPEHKRAGVVVIDLGGGT
                     TDFIVYNAGLVKLAGSIAVGGDHVTADICSGLNLNRRQAEAIKKESGSALINRMQNDQ
                     NISIPAEGSFSGRVVRATTLHTIINARMEEIFKLIAEKIEDDGLSGMLSAGVILTGGG
                     SAMEGITDLAQQVFNAPCQIGRIHDCVGLSTKQEGARFAAAIGCVRYAASQIKPELPI
                     RAAWRTWLSKLWGG"
     gene            complement(2400..3272)
                     /locus_tag="PKI68_06115"
     CDS             complement(2400..3272)
                     /locus_tag="PKI68_06115"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNX53301.1"
                     /translation="MAARRRKTPKIYYAKTKNRPSKKIIVRRGMVILLLTATSVALLA
                     GAFAGLKYTGSLFFSRNPKFELKTINITSDGRLPPEFFCERAGLQVGTNLFSLDLDKL
                     RQKLEEVPLVESVTIDRRLPDTLIINITERVAMSQIRWNPRALPFLVDRFGVILPMTR
                     SGQSLPLIEGLKLQNLRPGDRVENPGIRQCLDILAAADQLGLGSQVAFSSFDTRYPDF
                     VTAIVNNDVTVRFPLHSGRERLIRLVSVLQLSNEQGRRVKTIDLTPDGRNVPVTFYEP
                     EKTDNPEPSGATRK"
     gene            complement(3302..>4159)
                     /locus_tag="PKI68_06120"
     CDS             complement(3302..>4159)
                     /locus_tag="PKI68_06120"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNX53302.1"
                     /translation="KGGFSAEREVSLESGASIAKGLRKAGYTVTEIDVTSPDFAVPAG
                     IDAVFIALHGTYGEDGGVQSRLTKLGLPYVGADEEASRRSFDKLLTETCLREAGVPVP
                     ESETLRRGDRRTLSLPVAVKPPRQGSSVGCSLVFDEAEWAPAMADALKYDDEILVQQF
                     IPGREFTVGIVDGEVLPIVEIVTAAGWYDYTAKYKVDTTRYVIPAELDAGTAARMQAL
                     ALKTFKALGARGFGRVDFRMTPEGEQFVLELNTIPGFTSHSLLPKAAAAAGIEFSMLC
                     DRIMKTASL"
     assembly_gap    4160..4259
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4260..5656
                     /locus_tag="PKI68_06125"
     CDS             <4260..5656
                     /locus_tag="PKI68_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNX53303.1"
                     /translation="LPDRAVAPEWFRDAKLGIYCHWGVYSVPAKFSEWYPRFMFDTNS
                     PVYAYHTQKYGDPTVFPYHKLVPLFTAEKFNAAEWADVYKASGAQFAGMVTEHHDGFS
                     MWASKLTPWNVKDCGPKRDTLGELAKEIKARDMKLITTFHHARNLQRNAGPNNGGGYD
                     SHYIYDKRFATASDDPVLQKLYGNMETNAFYTMWFGKLKEVIDNYNPDIVWFDSWLNL
                     IPEPDRIKFCAYYLEQAKARNKEVVIACKQNDLPETVMVKDIEQGGHSEIYPKAWMTD
                     DTICFESWAYSVDDKVKPASMVLHSLIDIVSKNGCLLLNMAPRADGSIPDEQKKCLRE
                     LGAWLKVYGEAIYATRPWLVPGYGPTQPKPNPHGGVSTVNYYTAADYRFTMAKDGSAV
                     YLIVLGKPAAGTELPLICFAPHRYPLPRPVAKVVELSSGKEVPMDQYDNRCVLRYPDL
                     PYNQMANVFKIILK"
     gene            5671..7221
                     /locus_tag="PKI68_06130"
     CDS             5671..7221
                     /locus_tag="PKI68_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNX53304.1"
                     /translation="MTQRKLLGMCAALCLLGGAQLQAAETAPYQPTWESLAGHQPAPE
                     WLKDAKLGIYFHWGVYSVPAYRTEWYPRWMFYDSAIFTNFNCSDVFKHHVETYGHPSK
                     FGYHDFVPMFTGEHFNPEEWADLFVKTGAKFAGPVAEHHDGFAMWASKITPWNAKDRG
                     PHRDVVGEMLAALKKREMKTITTFHHERNLQRYKETWKQELEKWAQKSPRKSFPNSHF
                     PYIPGMPTASEDPELRMLYGNMPEAEWLEKMWLGKLVEVIDAYQPDIIWFDSWLDCIP
                     ENYRQRFCAYYLNAAAKWNKEVAIVRKQDDLPIGFTVNDHEKSREPNIQPALWMSDDT
                     VSTDSWSYTADMKIKPVADVLYSLLDIVSKNGVLLLNISPKADGTIPDDQRQLMLGLG
                     AWLKKNGEAVYSTRPWIIAAEGPTSSPKAGLNHSVEFLKLKYSAQDIRYTAAKNGKTV
                     YAITLGAPQPGEPICLNGFAKQIDQIKNVRLLNGQKLDWKKDGTTLRVTAPATDETLA
                     LAVAVELQ"
     gene            7272..9245
                     /locus_tag="PKI68_06135"
     CDS             7272..9245
                     /locus_tag="PKI68_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX53305.1"
                     /translation="MSKVKEINRRQFIGYSGAAALSLLAARSFAGDLNRPSNGQFLFL
                     EAEGFSEHGGWEIDQQSMDQMGSPYLLAHGLGVPVKDAVAQVQFPSAGTYRVWVRTKD
                     WVAPWNAPGAPGKFQLLINGVPLAETFGTKGAEWHWHNGGTVEVGGTARIALHDLTGF
                     EGRCEAVLFCKDTAFEPVNDVEALTRFRRKLLGFPDRPEYGGRYDLVVVGGGLAGICA
                     ALSAARRGLSVALVHDRPVLGGNGSSEVRVWPEGHTNKPPYPSIGDIVTEILKPINKA
                     TAEGKWFGTLNGTAASNFDDQRKLDVVKTEPRINLLNNHRAIQTLTSGNRIQAVVIQS
                     IRTGRQQLLEGTFFADCTGDATVGFQAGAEYEYAFSSGNPLMGSSNLFNVIDASNQKD
                     VLDCDCKDKSALMTQYQEGKYEQPFPRCPWALDLSDKPFPGRLKKQATGTLKDLKSFA
                     NAWYWESGFDKDQVNDIELIRDHNFRAFYGAWDALKNVDGLYKNFRLAWAAFIAGKRE
                     SRRLMGDVVLDGKHFTEMTDWADKAFPCSWHIDLHFPEKKYQAGFEGNEFISDFTRGK
                     DYKYGGLYWAPYRCLYSRNIGNLFMAGRNISVTKTGLGPVRVMKTCGMMGEVIGKAAA
                     LCVQHGTTPRGVYENHLAALQEMMKQPGSFRTA"
BASE COUNT         2074 a         2913 c         2680 g         1810 t
ORIGIN      
        1 agtttgattt tgcggcggat ataggtcggc acgtcgaggt cttcgccttc ccagatggtg
       61 cgttccgcgc cgccaaaggt gcgcgaacgc ggcttgagga caccggccag ctcgccctgt
      121 tttctgtcgc cggaaaccgg cttaagtccg ccgcgtccgt cgtccaccaa aggttctttc
      181 caggcttcag ccgccagcac aatggcggat atgcgtcccg taaacgccgg atcgatcgcg
      241 atgcccagct tgatccagca ttcttctttg tccggcgcaa tggtttcaac aatctgctga
      301 atttcggaaa gtttcagatc ttcgccgccg gtgatgccga tgatcatgcc gggcgctttt
      361 tcaaatacgg cagtgtcctt gaccagcgga tgggtgcgca gtgaatcgat cagtgcgtcg
      421 gtgcgtcctt cgccggtcgc cgtggcgcac gagaagcggc agaaagcgtc gcagtaactc
      481 agcatggttt gaagcgtcgc gaagtcgaga ttgcagatac cggtatacgc cagcatccgc
      541 cagatgccga tcacacccgc cgccagtgtg cggctactgc gttccagact gtcgccgaga
      601 atcgcattcc cttccggctg aatcagcgcg ttgggcatct gcacaatggc gtcggcatag
      661 gtgcgcagcc ggcggatcgc gctgtgtgct ctgccctgcg ccgtcaaccc ttcaaacggg
      721 aacggcagta cggtgaagaa cagcgttgtt gtgccgagat ggcgggccat acgggcaata
      781 accggagccg cgccggttcc gaagccgccg ccgagaccgg tgatgaccat cagaagatcg
      841 acgccgctca gcatcgtttc gatctgaccg gcggagtttt cagcgcagcg gcggccgact
      901 tcaaggtcgc cgcccgtact cataccgtct gtaagcgcct tgccggcggc tatacggttt
      961 tcaatattgg aacaggtttc catcgtctgg atatcgcagt ccataacggc gaattccatc
     1021 ccttcgggcg cccgcggcgc aatatgcgca atggtgttgc aacccgctcc gcccagaccn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cgccccagag tttggagagc
     1201 caggtccgcc acgcggcgcg gatcggcagc tccggcttga tttgactggc ggcataacgc
     1261 acacagccga tggcggcggc gaagcgcgcg ccttcctgct tggtggagag tccgacacag
     1321 tcgtgaatgc gcccgatctg acagggcgcg ttgaaaacct gctgggcgag atcggtgatg
     1381 ccttccatcg ccgagccgcc gccggtcaga atgacgccgg cgctgagcat tccggaaagt
     1441 ccgtcgtctt caattttctc ggcaatcagc ttaaagatct cttccatgcg ggcgttgatg
     1501 atggtgtgca gcgttgtagc acgcacaacc cgtccgctga aactgccttc ggcgggaatg
     1561 gaaatattct gatcgttctg catccggttg atgagagcgc tgccggattc ttttttgatc
     1621 gcttcggcct gccgccggtt aaggttgagt ccgctgcaga tatcggccgt gacgtggtcg
     1681 ccgccgacgg cgatggagcc ggccagcttg accagaccgg cgttataaac gatgaagtcg
     1741 gtcgttccgc cgccaaggtc gatcacgacg acgccggcgc gtttatgttc cggtgcaagt
     1801 accgcctgcg cagaacagaa tccgccgaac gcggcatcgg cacaaaccac cggcacgttt
     1861 tcggtcagct ttttaaggtt ctcgaccacg gcgcgctggc cgtgaatcag cagcatcccg
     1921 acacgcagct cttcagccag catccgctcc ggattaacga tgccgccgcg tccgtccact
     1981 tcaaagtgct gctgcagcgt atgcaggcgg atgcggtcgt ccggcagcgg aattttacgg
     2041 gccacttcga gcgcctgatc aatatcttcg cgtgtgatct cgccgccggg ctcttcatat
     2101 tcgttgagta ccgggattcc gccgacgtgc agcaggcttt cagcccgtcc gccggaggcg
     2161 accagataaa cgctttcgcc gatggattta tgcgcgaccg cttcggcggc ttccatggcg
     2221 cggcggacgg cgtcgagcgc cagttcgaag tcgacaatct cgcctttgcg cacaccgcgc
     2281 gattcgcatt cgcccagtcc ggcgatcatc aggccgccgt cttcgcggac ttcggccatc
     2341 aggaccttga tggtcgttgt accgatttca agtgcagtaa tgaagggtgc tgccatacat
     2401 catttccttg ttgcgccgga gggttcgggg ttatccgttt tttccggctc ataaaacgtg
     2461 accggaacat tccgtccgtc gggtgttaag tctattgttt tcacacggcg gccctgctcg
     2521 ttcgacagtt gcaggacgct gacgagccgt atcaatcgct cgcgcccgga atgcagcgga
     2581 aagcgaaccg tcacgtcatt attgacgatg gcggttacaa agtcgggata gcgggtgtca
     2641 aaactggaga aggcgacctg cgaaccgagc ccgagctgat cggccgcggc gagaatatcc
     2701 agacactgtc ggataccggg gttttcaacc cggtcgccgg ggcgcagatt ctgcaacttg
     2761 aggccttcaa tcagcggaag cgactgtccg ctgcgcgtca tcggcagaat aacaccaaag
     2821 cggtccacaa gaaacggcag cgcgcgcgga ttccagcgga tttgcgacat cgcaacccgt
     2881 tccgtgatat taatgatcaa ggtgtccgga agtctccggt cgatggtaac agattcaacc
     2941 aggggcacct cttccagctt ctgccgcagc ttatccagat ccagagaaaa caggtttgtt
     3001 ccgacctgca atccggcgcg ctcacagaaa aattccggcg gaagccgtcc gtcggacgta
     3061 atgttgatgg tcttaagttc aaacttcgga ttgcgggaaa aaaagagaga cccggtgtat
     3121 ttgagtcccg cgaaagctcc ggccagcagc gccacagatg tcgcggtcag aagaagaatc
     3181 accattccgc gccggacgat gatttttttc gaaggccggt ttttggtttt agcgtagtaa
     3241 attttcggtg tttttcgtct tctggcagcc attgcggttc tccgtcgcta tgctttttct
     3301 tttatagcga agcggttttc atgattcgat cacaaagcat ggaaaactca attcctgccg
     3361 cggccgccgc cttgggaagc aggctgtgcg aggtaaagcc cggtatggtg ttgagttcga
     3421 gtacaaactg ttcgccctcg ggcgtcatgc ggaaatcgac gcggccgaat ccgcgagcgc
     3481 cgagcgcttt aaaggttttc agagccagcg cctgcatccg cgccgcggtt ccggcatcga
     3541 gttcggccgg aataacgtag cgcgtggtat cgactttgta tttggcggta tagtcgtacc
     3601 agcccgccgc cgtcacgatc tcgacgatcg gcagcacttc gccgtccacg attccaacgg
     3661 tgaattcgcg gcccggaatg aactgctgta caagaatttc gtcgtcatat ttcagggcat
     3721 cggccatcgc cggagcccat tcagcctcat cgaaaaccag cgaacagccg acgctggaac
     3781 cctgccgcgg cggcttaaca gcgaccggca gtgaaagcgt acgccggtcg ccgcgccgca
     3841 gcgtttcgct ctccggcacc ggcacgcccg cttcgcgcag gcaggtttcg gtgagcagct
     3901 tgtcgaatga gcggcggctt gcctcttcat ctgcaccaac gtacggaagt ccaagttttg
     3961 tcagccgcga ctgcactccg ccgtcttcac cgtaggtgcc gtgaagcgcg atgaaaaccg
     4021 cgtcgattcc tgccggaacc gcgaagtccg gcgaggtgac atcaatttca gttacagtgt
     4081 atccggcttt gcgcaggcct ttggcgatcg acgcgccgga ttcgagcgag acttcgcgct
     4141 ctgcagaaaa gccgcccttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     4261 tctgccggac cgtgcggttg cgccggagtg gttccgcgac gccaagctgg gcatttactg
     4321 ccactggggc gtctactccg taccggcaaa attctccgag tggtatccgc gcttcatgtt
     4381 cgacaccaac tcgccggtct acgcctacca cacgcagaag tacggcgacc cgaccgtgtt
     4441 cccctaccac aagctggtgc cgctctttac cgccgagaag tttaacgccg ccgagtgggc
     4501 tgatgtctat aaagcgtccg gcgcccaatt cgccggcatg gtgacagagc atcacgacgg
     4561 cttctcgatg tgggcctcga aactgacgcc gtggaatgtc aaagactgcg gcccgaagcg
     4621 cgacaccctc ggcgagctgg ccaaggaaat taaagcccgc gacatgaagc tgatcaccac
     4681 cttccaccat gcgcgcaatc tgcagcgcaa tgccggaccg aataacggcg gcggctacga
     4741 cagccactat atttacgaca agcgcttcgc aaccgcctcg gacgatccgg tgctccagaa
     4801 gctgtacggc aacatggaaa ccaacgcgtt ctacaccatg tggttcggca aactgaaaga
     4861 agtcatcgac aactacaacc cggacatcgt ctggttcgac tcctggctga acctgattcc
     4921 cgagcccgac cggataaagt tctgcgccta ctatctcgaa caggccaagg cccgcaacaa
     4981 ggaagtggtg attgcctgca aacagaacga cctgcccgaa acggtgatgg ttaaagacat
     5041 cgaacagggc ggccattccg aaatctaccc gaaagcctgg atgaccgacg acacgatctg
     5101 cttcgagtcc tgggcgtaca gcgtcgatga caaggtcaag ccggcctcca tggtcctgca
     5161 cagcctgatc gacatcgtca gcaaaaacgg ctgcctgctg ctcaacatgg ccccgcgcgc
     5221 cgacggctcg attccggacg aacagaaaaa atgtctgcgc gaactcggcg cctggctcaa
     5281 ggtttacggc gaagccatct acgccacccg cccctggctg gtgcccggct acggccccac
     5341 ccagccgaaa cccaatccgc acggcggcgt cagcaccgtc aattactaca ccgccgccga
     5401 ctaccgcttc accatggcca aagacggcag cgccgtctac ctgatcgtgc tcggcaaacc
     5461 ggcggccgga acggaactgc ccttgatctg cttcgcgccg caccgctacc cgctgccccg
     5521 cccggtcgcc aaggtcgtcg aactctccag cggcaaagag gtgccgatgg atcagtacga
     5581 caaccgctgt gtgctgcgct acccggacct gccctataac caaatggcca acgtatttaa
     5641 aatcattctg aaataataaa ggaactcaac atgactcaac gcaaacttct tggaatgtgc
     5701 gcggcgctct gcctgctcgg cggggcgcag cttcaggcgg cggaaaccgc tccgtaccag
     5761 ccgacgtggg aatcgctcgc gggccaccag ccggcgccgg aatggctgaa agatgccaag
     5821 ctggggatct acttccactg gggcgtctat tcggtgccgg cctaccgcac cgaatggtac
     5881 ccgcgctgga tgttctacga ctccgcgatc ttcaccaact tcaactgttc ggatgtcttc
     5941 aagcatcacg tcgaaaccta cggacatccc tcgaagttcg gctaccacga cttcgtcccg
     6001 atgttcaccg gcgagcattt caacccggaa gaatgggccg acctgttcgt taaaaccgga
     6061 gctaaattcg ccggccccgt cgccgaacat cacgacggct ttgccatgtg ggcctcgaaa
     6121 atcacgccgt ggaacgccaa agatcgcggc ccgcaccggg atgtcgtcgg cgaaatgctc
     6181 gcggcgctca aaaagcggga gatgaaaacc atcaccacct tccaccacga acgcaacctg
     6241 cagcgctaca aagagacctg gaaacaggaa ctggaaaagt gggcgcagaa gagcccgaga
     6301 aaatctttcc cgaacagcca cttcccctat atcccgggaa tgccgactgc atcggaagat
     6361 cccgaactgc gcatgctcta cggcaatatg ccggaagctg agtggctgga aaaaatgtgg
     6421 ctcggaaaac tggtcgaagt catcgacgcc tatcagccgg acatcatctg gttcgacagc
     6481 tggctcgact gcatccccga aaactatcgc cagcgcttct gcgcctatta cctcaacgcg
     6541 gccgccaaat ggaacaaaga ggtcgccatc gtccgcaaac aggacgacct gccgatcgga
     6601 ttcaccgtca acgaccacga aaagtcgcgc gaaccgaaca tccagcccgc gctgtggatg
     6661 agcgatgaca ccgtcagcac cgacagctgg agctataccg cggacatgaa gatcaagccg
     6721 gtcgccgacg tgctctattc gctgctcgat atcgtcagca aaaacggcgt gctgctgctc
     6781 aacatctcgc cgaaagccga cggcaccatt ccggacgacc agcgccagct gatgctggga
     6841 ctgggcgcct ggctgaaaaa gaacggcgaa gcagtgtaca gcacccgacc ctggatcatc
     6901 gccgccgaag gcccgaccag ctcgcccaag gccggactca accactccgt tgaattcttg
     6961 aagctgaaat attcggctca ggacatccgc tacaccgcgg cgaaaaacgg caaaaccgtt
     7021 tatgccatca ccctcggcgc cccgcagccc ggcgaaccga tctgcctgaa cggctttgcc
     7081 aaacagattg atcaaatcaa aaacgtccgc ctgctcaacg gacagaaact ggactggaaa
     7141 aaagacggca caacgctccg cgtcaccgca ccggctaccg acgaaacgct cgcgctggcc
     7201 gtcgccgtcg aactgcagta aatcgccggc cagagcaacg gacacaggaa tctaacgcag
     7261 agggcccagc catgagtaaa gttaaagaaa tcaaccgtcg gcagttcatc ggatattcag
     7321 gagccgccgc cttgagcctt ctcgcagcga gaagcttcgc cggagacctg aaccgtccgt
     7381 ccaacggtca attccttttt ctggaggccg aaggattcag cgagcacggc gggtgggaaa
     7441 tcgatcagca gtcgatggat caaatgggtt cgccgtacct gctggcccat ggattaggcg
     7501 ttccggtcaa agatgcggtc gcccaggttc agttcccttc ggcgggcacc taccgggtct
     7561 gggtgcggac caaagactgg gttgccccgt ggaacgcacc gggcgcgccg ggaaaatttc
     7621 agctgctcat caacggagtt ccgctggccg aaaccttcgg caccaaaggg gccgagtggc
     7681 actggcataa cggcggcacg gttgaagtgg gcggaaccgc ccgcatcgcc ctgcacgatc
     7741 tcaccggctt cgaaggccgg tgcgaggccg tcctcttttg caaggacacc gcgtttgaac
     7801 ccgttaacga cgtggaagcg ctgacccgtt tccgccgcaa gctgctcggc tttccggatc
     7861 gtccggagta tggcggacgc tatgacctgg tcgtggtcgg cggagggctg gccggaatct
     7921 gcgccgccct gtcggctgcc cgccggggac tctctgtcgc gctggtacac gaccgcccgg
     7981 tgctgggcgg gaacggcagc tccgaagtgc gcgtctggcc ggaaggacac accaacaagc
     8041 ccccgtaccc ctctatcggc gacatcgtca ccgaaatcct gaagcccatc aataaagcga
     8101 cggcggaagg caagtggttt ggaactctga acggcaccgc ggccagtaac ttcgacgatc
     8161 agcggaagct ggatgtggtg aagacggagc cccgaatcaa cctgctgaac aatcaccggg
     8221 ccattcaaac gctgacctcg ggcaaccgga ttcaagcggt ggtcatccaa agcatacgca
     8281 ccggccggca gcaacttctg gagggaacct ttttcgccga ctgcaccggc gacgccaccg
     8341 tcggctttca ggccggcgca gagtatgaat atgcgttttc ctccggaaac ccgctcatgg
     8401 gcagcagcaa tcttttcaat gtcatcgacg cctcgaatca aaaggatgtg ctcgactgcg
     8461 actgcaaaga caagtctgcg ctcatgacgc aatatcagga agggaaatac gagcagccgt
     8521 tcccccgctg cccctgggcc ctcgacctct cggataaacc ctttccggga cggctcaaaa
     8581 aacaagccac aggcaccctc aaagacctca agagctttgc caatgcgtgg tattgggaga
     8641 gcggattcga caaagaccag gtgaacgata ttgagctgat ccgcgaccac aacttccgcg
     8701 ccttctacgg cgcatgggat gcgctgaaaa atgtcgacgg gctctacaaa aatttccgcc
     8761 tcgcatgggc cgccttcatt gcgggcaaac gcgaatccag acgtctcatg ggcgatgtgg
     8821 tgctcgacgg gaagcatttc accgaaatga cggattgggc cgacaaggcc ttcccctgct
     8881 cctggcacat cgacctgcac ttccctgaga aaaaatatca ggcagggttc gaaggcaatg
     8941 agttcatttc cgactttacc cgaggaaagg actacaaata cggcggactc tactgggcgc
     9001 cctaccgctg tctgtacagc cgcaacatcg gcaacctctt tatggcgggc cgcaacatca
     9061 gcgtgacaaa aaccggactc gggccggtgc gcgtcatgaa gacctgcgga atgatgggcg
     9121 aggtgatcgg caaagccgcc gcgctctgcg tccagcacgg aacaacccct cgaggggtct
     9181 acgaaaacca tttagcggcc ctgcaggaaa tgatgaaaca gcccggatcc ttccggaccg
     9241 cataagccgg aaacggccaa acatcatcac ctgctgaaaa ggtgcaaagg aacccctgag
     9301 tgacgggcca tcggtgtctc aaaccatggc ccgctcattc tccgcccgcg atgctcaatc
     9361 aaagatcccc gtccgggagg acaagttcgc gcgccgatcc gccgacgtcg aactcatcgg
     9421 gaacgattcg ccgttggcgc tgttcgtaat cgtcatcgca agcagccggc tcatcgcatc
     9481 aaatcttcaa agagaacaat aaaaacaacc ggatcaaatt ctaagattca ttggcccctc
     9541 ctttttttgt ggaacagacg gctgaaggtg cggcaacggt tgaggcgacg tttacaagga
     9601 gtaaaaggaa gccgcttact cagcggaacg gagcgaattt ttgtgtttga ccgagatctt
     9661 taatgggtgt ccttttt
//
LOCUS       DAOPZO010000076         9566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_8392_length_9566_cov_8.991799, whole genome shotgun sequence.
ACCESSION   DAOPZO010000076 DAOPZO010000000
VERSION     DAOPZO010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 9566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9566
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8392_length_9566_cov_8.991799"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PKI68_06140"
     CDS             <1..522
                     /locus_tag="PKI68_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX53306.1"
                     /translation="QQRAMRQLALVVPMALLLILVLLYLALGSVSSSLLVLLNLPFAL
                     IGGIVAVVAFRMPLSVSAAVAFIVLLGIAVQNGVILAAFFRQLRDRGRSVDETIREGC
                     DLRFRPLLMTALTSFIGHLPMLYATGAGADIQKPLAVVVMGGLITSTLLTLIVLPTIY
                     GLVERRKERLKIG"
     gene            628..1809
                     /locus_tag="PKI68_06145"
     CDS             628..1809
                     /locus_tag="PKI68_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNX53307.1"
                     /translation="MSKIAIKNDIFWVGALDPDRKVFDGLLPLPQGTTYNSYLVVGSE
                     KTALIDTVEPEFIDVLLANLEGVKKIDYIVSHHAEQDHSGSIPVLLEKYPEATLLCLQ
                     KNKEMLKDLMTIPDDRIQVVQDGGEVSLGNKTIRFMFCPWVHWPETAITYIPEDKILF
                     PCDFLGSHHSFTTIFAGDNPAVYKGAKEYYVQIMMLYSKMTAKYLDRIKALDIEYICP
                     SHGSVYDKPQIILGLYEKWMRGEPENLALIAYVSTHGSTKLMAEHLQTALEKRGVNVE
                     LKDLVTVPLNELAGLLTDAATIVLGSSVVMAALHPVAVNTAFLLDRLKPKAKFAAVIG
                     SYGWSPGPLQKAAELLPSWKVDVVGAVITKGHPGEQAMAELDALADTIAAKHRGAGIL
                     K"
     gene            1825..>2687
                     /locus_tag="PKI68_06150"
     CDS             1825..>2687
                     /locus_tag="PKI68_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNX53308.1"
                     /translation="METNAMQNAVNGVQQMFNNTPEWVTTAQAMLTAFGLKLVAAILI
                     FVIGRWVAKMVQNGLEKGMLKAKVDQVLVTFTVNMAYTALMVFVALAALGQLGVQTTS
                     FIAVLGAAGLAVGLALQGSLSNFASGVMIIIFRPFKIGDYVEGGGTGGTVKAIHIFTT
                     TLTTGDNKRVIVPNSKMMGDNIINYSAEGTRRLDLTASIGYGDSIDKAKAVLQDILAK
                     EPRVLKDPAPFVGVSKLAESSVDFTVRPWVQSADYWPVCFDLNEAIKKRFDAEGLSIP
                     FPQRDVHLYKH"
     assembly_gap    2688..2787
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2788..3669)
                     /locus_tag="PKI68_06155"
     CDS             complement(<2788..3669)
                     /locus_tag="PKI68_06155"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNX53309.1"
                     /translation="MKTGNKVRILINPNSGMGVALDRIHEALIKYWDLPGNDLAIQFS
                     TSKEDGQAKARRAVEEGVGKLLVIGGDGMVNSIGSVLIGTNTALGVIPAGSGNGFARH
                     FDIPLDLVKATEALATASVQRIDVGFANEQPFFVTCSLAWDAALVNIFEKSPVRGVLP
                     YIFAGVYQFFDYTPQPFTFCIDDGDEFTIDDPQVCTAANLTQYGGGAQIAPGAKADDG
                     QLLLVTVRKRDLPFVVLMIGKLFDGTIDRLSGIHTRPFRTLTVRRKNPGPMQLDGELI
                     AAPAEVKISVRPRALNVL"
     gene            complement(3672..4217)
                     /locus_tag="PKI68_06160"
     CDS             complement(3672..4217)
                     /locus_tag="PKI68_06160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNX53310.1"
                     /translation="MIRALIPLAEGCEEMEAVIVADTLRRAGWNVVLAGLHGTAPVTA
                     SRKVNIVPDARWEELDLLSFDLLVLPGGAGGTKVLCEHDGVQEAIRVFDIEEKWIAAI
                     CAGPLALHKAGVLKGRAFTCFPGVEKEMHRTDRSDGTVVVDRNLVTSQGPGTAFAFAL
                     RLIELLESSSAAEKVRAGLIY"
     gene            complement(4228..4962)
                     /locus_tag="PKI68_06165"
     CDS             complement(4228..4962)
                     /locus_tag="PKI68_06165"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HNX53311.1"
                     /translation="MNSEKTTDRFAKVYGSPGQGPRTLGPLRVFWPLIPICLAAGWLL
                     RAAAPVPEISTSQAGALFVLLAVTLVVFIGWSAKRLRSFLKGAEGEETVARILSFLPA
                     NHTVFNDLQLDPGGPAFDHIVVAPAGIFVIETKTWSGEITFQNGQVLCNGHLPSRPPL
                     KQVREEAAALVDHLAAAGCPAAPVYPVVCFVGNRPQDGHANIGGVRICTETDLRTLFE
                     NTLETPIPAGTLGMITSELARCAEDK"
     gene            5063..8095
                     /locus_tag="PKI68_06170"
     CDS             5063..8095
                     /locus_tag="PKI68_06170"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNX53312.1"
                     /translation="MDKKPFFRRADWISFGLTFLIALIVYTLTLAPTVTLEDSGELIT
                     ASDYLGVPHPPGYPIWSLLTWFFQWIFNFVTYRGYPNPAWGVAFCSAFAGALACGVLA
                     LLVSRSGMDILHSLKKENSVLGEKTESLFCCVAGIAGGLLLAFSQGLWSQSVIAEVYA
                     LNILFQTVVLLFLYRWMMEPENPKPLLLMAFVFGLGLTNHQTLLFMGVGVALAVLLRN
                     IRLGYSFAAVGFFYVLMVALNSLMAGNPQYASWCWVSGPSTVGFWFWTVYAVVVPVLG
                     LKLPHGKTVCATYLLMLLGLSFYLYMPISSDQNPPINWGYPRTWEGFMHAITRGQYEK
                     VTLTDVFSPMFLEQIGAFLHDLRAQFFGPVALLAILPFAFVVRIGRKNRDWLFTTVAA
                     FIGVGLLFIMLQNPKLDIQTLFIARVQYIQSHAIYAIWIGYGLLLLMAWLESLVGNNP
                     VTKWVGTLMVFLLPFSLIHKNYYNKEQLKVVGGSEQNGHDFGWQFGNWELQGIEGIKE
                     DLHYKGSFPFERRLSDAEFEKVWNEYPETLRKNNYPPPMGTNALFFGGTDPGRFVPTY
                     MIYCPGVRPDVYLITQNALADNTFMAVTRDLYGDQLWIPSQLDSNRAFQDYVQAIQSG
                     KLDAGADVKMEGGRVQVTGVGGVMQINGILCRQIFDHNQYVTEAKTDEATRQSGAAVV
                     PFDPVGPDGKLRKRDFYVEESYVIAWMYPYLTPHGLIMKINNEPTEITPEMVKNDHEF
                     WDWYVKRLVGDSKFTRDIVARKTFSKLRSAIAGLYAVRGNMAEAEYAFKQAVELYPLS
                     PEAVFRLADLYMRERRLDDSYKVVSEFAAQDVHNQSARGFLKQIDQTRQMFSRKAELE
                     ALLRAGKSTDINNAFELIQIYSALQEIEPMRQLIGSVLQSGLPPEVNLQLAQLLVQIK
                     RVEFVEPIFINYLQARPDDLRIQLELAALRLSLGHPDLAIETLRTAVQKGGEPICAVL
                     RKDARFQPLWNDARFQAIVPPIPAAGRRLPSGIGGLSL"
     gene            8109..8933
                     /gene="lpxI"
                     /locus_tag="PKI68_06175"
     CDS             8109..8933
                     /gene="lpxI"
                     /locus_tag="PKI68_06175"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HNX53313.1"
                     /translation="MNETPETLALIAGRGDYPLLLARAARARGVKRIFAVAFKGETCR
                     EIEAVADKVVRLHVGSFGALLDTLKESGAKHVVMAGQINPKNLFCVRMDKTLLDLLKS
                     LPVKNAHTIFGSITDEIEKTGLKLLPASSFMQDYMPAAGLLTARAPDAREVNDIAIGR
                     RVIKDTSHLDIGQTVVVKDGMILAVEAFEGTDKAIRRGGKLGGRGAVVVKVPKHGHDM
                     RFDIPVIGSRTLQSLRKAKATCLAIEEGGAILLHKDELIARADAMGLAVTVLKAQG"
     gene            8937..>9566
                     /locus_tag="PKI68_06180"
     CDS             8937..>9566
                     /locus_tag="PKI68_06180"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53314.1"
                     /translation="MKSLLVIAGEDSGDMHAADVVRALKEKRSDLTVWGIGGDKLRAE
                     GMELLHDTREMDVLGIVEVLKRYPFFKRTFNEVLVEADKRRPDAALLVDYPGFNLRLA
                     RELKKRGIKVLYYVCPQVWAWNRGRIPKMAETIDRLMVIFPFEVDVFKGVDLKVDFVG
                     HPMVDELREFREKAAEPLPWNGKKKIALLPGSRKQEIIYILPRLLEAAQL"
BASE COUNT         1903 a         2620 c         2836 g         2107 t
ORIGIN      
        1 cagcagcgcg ccatgcggca gctcgcgctg gtggtgccga tggcgctgct gctgattctg
       61 gtgctgctct atctggcgct cggctccgtt tccagttctc tgctggtgtt gctgaacctg
      121 ccgttcgcgc tgatcggcgg catcgtggcg gtcgtggcct tccggatgcc gctctcggtt
      181 tcggcggccg tggccttcat cgtactgctc gggattgccg ttcagaacgg cgtgattctc
      241 gcggccttct tccggcagct gcgtgaccgc ggccggtcgg tggacgaaac gatccgcgaa
      301 ggctgcgacc tgcggttccg cccgctgctg atgacggcgc tgaccagttt tatcgggcat
      361 ctgccgatgc tctacgccac cggagcgggc gcagatattc agaagccgct ggcggtggtc
      421 gtcatgggcg gactcatcac gtccaccctg ctgacgctga tcgttctgcc gacgatttac
      481 ggcctcgtcg agcggcgtaa ggaacgcctt aaaatagggt gaattggata cgtttattac
      541 cggaatgcgg taactggccg cattccggga ttgtaaaccg cgggcgctct gtggaaactt
      601 gcggcactaa cttgctggag gagtaccatg agtaaaatcg ctattaaaaa tgatattttc
      661 tgggttggcg cgctggatcc ggaccgtaag gtgtttgacg ggttgctgcc gcttccgcag
      721 ggaaccacct ataattccta tctggtggtc ggttcggaaa agaccgcttt gatcgatacg
      781 gttgagccgg aatttattga tgtcctgctg gcgaatctcg aaggcgttaa aaaaatcgac
      841 tacatcgtgt cgcatcacgc cgagcaggat cattccggat cgattcccgt cctgctggag
      901 aaataccctg aggcaacgct gctctgcctg caaaaaaaca aagagatgct taaggatctg
      961 atgacgattc cggacgaccg gattcaggtt gttcaggacg gcggcgaagt gtcgctcggc
     1021 aacaaaacga tccgctttat gttttgtccg tgggtgcact ggccggaaac ggcgatcacc
     1081 tatattccgg aagataaaat tctcttcccg tgcgacttcc tcggctcgca tcattccttc
     1141 accactattt tcgcgggcga caatcccgcc gtttacaaag gcgcaaaaga gtattacgtc
     1201 cagatcatga tgctctattc caaaatgacg gcgaagtatc tcgaccgcat taaggcgctg
     1261 gatatcgaat acatctgtcc gagccacggc tccgtctacg acaagccgca gatcattctc
     1321 ggcctctacg aaaagtggat gcgcggcgaa ccggaaaacc tcgcgctgat cgcctatgtc
     1381 tcgacgcacg gcagcacaaa actcatggcc gaacaccttc agacggcgct tgaaaaacgc
     1441 ggcgtgaacg ttgagctgaa agatctggtc accgttccgc tcaatgaact ggccggtttg
     1501 ctgaccgacg cggcgaccat tgtgcttggc tcatccgtcg ttatggccgc actgcatccg
     1561 gttgctgtca ataccgcgtt tctgctcgac cggctgaaac ccaaggcaaa atttgcggcg
     1621 gtgatcggtt cgtacggctg gagtccgggg ccgctgcaga aagccgcgga actgctgccg
     1681 tcctggaaag tggacgtcgt cggcgcggtc attacaaaag ggcatccggg cgaacaggct
     1741 atggctgagc tcgacgcgct ggccgatacc atcgccgcca aacatcgcgg agccgggatt
     1801 ctgaagtaat agaaggagat tctgatggaa acgaatgcaa tgcagaatgc ggtgaacggc
     1861 gtgcagcaga tgttcaataa cacaccggag tgggtgacga ccgcgcaggc gatgctgacg
     1921 gccttcggtc tgaagctggt tgcggcgatt ctgatttttg tcatcggtcg ctgggttgcc
     1981 aaaatggttc agaacggcct cgaaaagggc atgctgaagg ccaaggtcga tcaggtgctg
     2041 gtgacgttca ccgtgaacat ggcgtatacc gcgctgatgg tttttgtggc gctggccgcg
     2101 ctgggacagc tcggcgtgca gaccacttcg tttattgccg tgctgggcgc cgccggcctg
     2161 gccgtcggtt tggcgttaca gggttcgctc tccaatttcg cctcgggcgt gatgatcatt
     2221 attttccgcc cgttcaaaat cggcgactac gtggagggcg gaggcacggg cggcaccgtg
     2281 aaggcgattc atatcttcac gacgacgctg acgacgggcg acaataagcg ggtgattgtc
     2341 ccgaactcga agatgatggg cgacaacatc atcaactact ctgccgaagg cacccgccgg
     2401 ctcgatctga ccgccagcat cggctatggc gacagcatcg acaaagccaa ggcggtcctg
     2461 caggacattc tggcgaaaga gccgcgcgtg ctgaaggacc ctgcgccgtt tgtcggtgtt
     2521 tccaagctgg cggaaagcag tgtcgacttc actgtgcgcc cctgggttca atcggccgat
     2581 tactggccgg tttgctttga tctgaatgaa gcgatcaaaa agcgctttga tgccgaaggg
     2641 ctgtcgattc cgttcccgca gcgcgacgtg catctttata aacacgannn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnncag aacgttcagc gcgcggggcc gcacgctgat
     2821 tttgacttcc gccggggccg caatcagttc gccgtcgagc tgcatcggtc ccggattttt
     2881 tctgcgcacc gtcagtgtgc ggaacggacg ggtgtgtatg ccgctcagcc ggtcgatggt
     2941 gccgtcaaaa agttttccga tcatcagcac gacgaacggc aggtcgcgct tgcgcacggt
     3001 cacgagcaga agctgtccgt cgtcggcctt ggcgccgggg gcgatctgcg cgccgccgcc
     3061 gtactgggtg aggttggcgg cggtgcagac ctgcgggtcg tcgatggtga actcgtcgcc
     3121 gtcatcaatg cagaaggtaa acggttgcgg cgtgtagtcg aagaactggt acaccccggc
     3181 gaaaatatag ggcaggacgc cgcgcaccgg cgatttttca aaaatgttta ccagcgcggc
     3241 gtcccaggcc agcgagcagg tgacgaaaaa cggctgttcg ttggcaaagc cgacatcaat
     3301 gcgctggacg gatgccgtgg cgagcgcttc cgtggcttta acgaggtcga gcggaatatc
     3361 aaagtggcgc gcaaatccgt tgccgcttcc ggcgggaatg acacccagcg cggtgttggt
     3421 gccgatcagt acgctgccga tggagttgac cattccgtcg ccgccgatca ccagcagttt
     3481 gccaacgcct tcctccacgg cacggcgcgc tttggcctgt ccgtcttctt tggaggtgct
     3541 gaactggatg gccagatcgt tgcccggaag atcccagtat ttaatcagag cttcgtgaat
     3601 acgatccaga gcgacgccca ttccggaatt cgggttgatc aggatgcgga ctttatttcc
     3661 ggttttcatg ctcagtagat taatccggcg cggacttttt ccgccgcaga agagctttcg
     3721 agaagttcga tcaacctgag cgcgaaggcg aacgctgtgc cggggccctg gctggtgacg
     3781 agattgcggt cgacgacgac ggttccgtcc gaacggtcgg tgcggtgcat ctctttttca
     3841 acgcccggaa agcaggtgaa ggcgcggccc ttgagtacgc ccgctttatg gagcgccagc
     3901 ggtccggcac agatggcggc aatccatttc tcttcgatgt cgaaaacgcg gatggcttcc
     3961 tgtacgccgt cgtgttcgca aagaactttc gtgccgcccg cgccgccggg cagcacgagc
     4021 aggtcaaaag acagcaggtc gagttcttcc cagcgggcat ccgggacaat atttactttg
     4081 cgcgaggcgg tgacgggggc ggtgccgtgc agtccggcca gtacaacatt ccagccggcg
     4141 cgccggagcg tatcggcgac aatgacggct tccatttctt cgcagccttc agcgagcgga
     4201 atcagtgcgc ggatcatggc ggagctccta tttatcctct gcacagcgcg ccagttcact
     4261 ggtgatcatg ccgagcgttc cggccggtat gggcgtttcg agggtatttt cgaaaagagt
     4321 gcgcagatcg gtttcggtgc agatgcgcac accgccgata ttggcgtgac cgtcctgcgg
     4381 acggttgccg acgaaacaga cgaccggata gaccggcgct gccgggcatc ccgcagcggc
     4441 aaggtggtcg accagcgcgg ccgcttcttc gcggacctgt ttgagcggcg gacggctggg
     4501 tagatgtccg ttacagagca cctgtccgtt ctggaaggtg atctcgccgc tccaggtttt
     4561 ggtttcgatg acaaagattc cggcaggtgc gacgacgata tggtcaaagg ccgggccgcc
     4621 gggatcaagc tgcaggtcgt tgaagaccgt atggttcgcc ggcaggaagg agaggatacg
     4681 cgcaaccgtt tcttcgcctt cggcgccttt gaggaagctg cgcagccgct tcgcgctcca
     4741 gccgataaaa accacgagtg ttaccgccag cagaacaaag agcgcaccgg cctgtgatgt
     4801 ggaaatttcc ggaaccggcg cggcggcacg cagcagccat ccggcagcga ggcagatggg
     4861 aatcaaaggc cagaaaacgc gcagcgggcc gagagttcgg gggccctgtc cgggactgcc
     4921 gtagactttt gcaaagcggt cggtggtttt ttcactgttc atggcgtgaa catacaggag
     4981 tccgggctgt ctgtaaactt gacctttaac cctaaaaaag gcttatcttt ccagctctaa
     5041 cattcgaacg aaaggcccca tcttggataa gaaaccgttt ttccgccgcg ccgactggat
     5101 ctctttcggt ctgacatttc tgatcgcgct gatcgtttac acactgacgc ttgcgccgac
     5161 cgtcactctg gaggactccg gcgaactgat taccgcttcc gattatcttg gcgtgccgca
     5221 tcctcccggc tatccgatct ggtcgcttct gacctggttt ttccagtgga tttttaattt
     5281 tgtaacgtat cgcggttatc cgaatcccgc atggggcgtg gcgttttgtt ccgcgtttgc
     5341 cggtgcgctg gcctgcggtg tgctggctct gctggtaagc cgctccggta tggacatcct
     5401 tcacagtctc aagaaagaaa acagtgtgct gggtgaaaag accgagagcc ttttctgctg
     5461 cgtagccggt attgccggcg gcttgctgct ggccttcagt caggggttgt ggtcgcagtc
     5521 tgttattgcg gaagtttatg cgctcaacat tctcttccag acggtcgtac tgctttttct
     5581 ttaccgctgg atgatggagc cggaaaatcc caagccgctg ctgctgatgg cctttgtttt
     5641 cgggttgggc ctgaccaatc accagacgct gctgtttatg ggcgtcggtg tggccctggc
     5701 ggtgctgttg cgcaatatcc ggctgggcta cagctttgcc gcggtcggtt tcttttacgt
     5761 gctgatggtg gcgctgaaca gcctgatggc cgggaatccg cagtacgcct cctggtgctg
     5821 ggtttccggt ccgtccacgg tcggtttctg gttctggacg gtttatgccg tggtggttcc
     5881 ggtgctggga ttgaaactgc cgcacggcaa gaccgtttgc gccacctatc tgctcatgct
     5941 gctggggctg tcgttctatc tttatatgcc gatctcttcc gatcagaacc cgccgatcaa
     6001 ctggggctat ccgcgcacat gggaaggctt catgcacgcc atcactcgcg ggcagtatga
     6061 aaaagtcacg ctgaccgacg tgttttcgcc gatgtttctg gagcagatcg gagcctttct
     6121 gcacgacctg cgcgcgcagt tcttcggacc ggtcgctctg ctggcgattc tgccgtttgc
     6181 tttcgttgtg cgcattggcc gtaaaaaccg cgactggctg tttacgacgg tggcggcttt
     6241 catcggcgtc ggccttcttt ttattatgct gcaaaacccg aagctcgata ttcagacgct
     6301 gtttattgcg cgggtgcagt acattcagtc gcacgctatt tacgccatct ggatcggcta
     6361 cggtctgctt ttgctgatgg cgtggcttga atcgctggtc ggtaataatc cggtcaccaa
     6421 atgggtcgga acgctgatgg ttttcctgct gccgttttcg ctgattcaca aaaactacta
     6481 taacaaggaa cagctcaaag tcgtcggcgg ctcggagcag aacggtcacg acttcggatg
     6541 gcagttcggt aactgggagc tgcagggcat cgaaggcatc aaagaagacc ttcactataa
     6601 gggctcattt ccctttgaac ggcgcctctc tgatgctgag tttgaaaagg tatggaacga
     6661 atatcctgaa actctgcgca agaacaacta tccgcctccg atgggcacca atgcgctctt
     6721 cttcggcggt accgatccgg gacgctttgt gccgacgtac atgatttatt gtccgggagt
     6781 ccgtccggat gtgtatctca tcacccagaa cgcgctggcg gacaatacct tcatggccgt
     6841 cacccgcgac ctctacggcg atcagctctg gattccttcg cagctcgaca gcaaccgcgc
     6901 ctttcaggac tacgtgcagg ccattcaaag cggcaagctt gatgccggtg cggatgtcaa
     6961 aatggagggc ggacgggttc aggtgacagg tgtcggcggc gtcatgcaga ttaacgggat
     7021 tctctgcaga cagatttttg atcacaacca gtatgtcacc gaagcgaaaa ccgacgaggc
     7081 cacccgccag tccggcgcgg cggttgttcc ctttgatccg gtcgggccgg atggcaagct
     7141 gcgcaaacgc gatttttatg tcgaagaaag ctacgtcatc gcgtggatgt atccgtatct
     7201 gacgccgcac ggcctcatca tgaagatcaa caatgagccg acggagatca ctcctgaaat
     7261 ggtcaaaaac gaccacgagt tctgggattg gtatgtgaaa cggcttgttg gcgattccaa
     7321 atttacgcgc gatatcgtgg cccggaaaac tttttccaag ctgcgcagcg ccatcgcagg
     7381 actctatgct gtgcgcggca atatggctga ggcggaatac gcctttaaac aggcggttga
     7441 actttatccg ctgagcccgg aagcggtttt ccgcctcgcc gatctttata tgcgcgaacg
     7501 ccgtttagac gactcgtaca aggtggtttc tgagtttgcc gctcaggatg ttcacaacca
     7561 gagcgcgcga ggatttctta agcagattga tcagacaaga cagatgtttt cccggaaagc
     7621 cgagctcgaa gctctgctgc gtgccggcaa gagcacagac atcaacaacg cctttgaact
     7681 gattcagata tacagtgcgt tgcaggaaat cgaacccatg cgccagctga tcggcagtgt
     7741 acttcagtcc ggtctgccgc cggaagtcaa ccttcagctc gcgcagttgc tggttcagat
     7801 caagcgggtt gaattcgtcg agccgatctt catcaattat ctgcaagcga gacctgatga
     7861 tctgcgcatc cagctggaac tggctgcctt gcgcctgtcg ctgggtcatc ccgacctggc
     7921 gattgagacg cttagaacag cggttcagaa aggcggcgag ccgatctgtg cggtactgcg
     7981 gaaagacgcg cgtttccagc cgctgtggaa tgatgcacgc tttcaggcga ttgtacctcc
     8041 gattcctgcg gccggccgcc ggctgccgtc cggaatcggc gggttgtcac tgtaggggat
     8101 tttcagccat gaacgaaaca cccgaaaccc ttgccctgat cgccgggcgg ggtgattacc
     8161 cgctgctgct tgcccgcgcc gcccgcgccc gcggcgtgaa gcgcattttc gccgtcgcct
     8221 tcaaaggcga gacctgccgt gagatcgaag cggtggcgga taaagtggtt cggttgcatg
     8281 tcggcagttt cggtgcattg cttgatacgc tcaaagaaag cggagcaaaa cacgttgtca
     8341 tggccgggca gattaacccg aaaaatcttt tctgcgtccg gatggataaa actcttctcg
     8401 acctgctcaa aagccttccg gtcaaaaatg cgcacaccat cttcggcagc attacggatg
     8461 aaattgaaaa aaccggtttg aaacttctgc ccgccagctc gtttatgcag gactacatgc
     8521 ctgccgccgg actgcttaca gcgcgcgcac cggatgcccg cgaagtcaac gacatcgcca
     8581 tcggccgccg tgtgatcaaa gacaccagcc atctcgacat cggccagacg gttgtcgtca
     8641 aagacggaat gattctcgca gtcgaagcgt ttgaaggcac cgacaaagcc atccggcgcg
     8701 gcggtaagct cggcgggcgc ggcgcggtcg tcgtcaaagt tccgaaacac gggcacgaca
     8761 tgcgcttcga tatcccggtc attggaagcc ggacgcttca atcgctccgg aaagccaaag
     8821 ccacctgtct ggcgatcgaa gagggcggcg ccatccttct gcacaaagac gaactcattg
     8881 cccgggccga tgccatgggc cttgccgtta ccgtgcttaa agcgcagggg taatcgatga
     8941 aatcacttct ggttatagca ggcgaagatt ccggcgacat gcacgcggcg gatgtcgtcc
     9001 gtgcgctgaa ggagaaacgt tctgatttga ccgtttgggg aatcggcggc gacaaactcc
     9061 gcgccgaagg catggaactg ttgcacgaca cccgcgaaat ggatgtgctt ggtattgtcg
     9121 aagtgctcaa gcgctacccg ttttttaagc gcacctttaa tgaagtgctg gtcgaggcgg
     9181 ataagcgcag gccggacgcc gctctgctgg tggactatcc cggtttcaac ctgcgccttg
     9241 cccgcgagct gaaaaagcgc ggcatcaaag tgctctacta cgtctgcccg caggtctggg
     9301 catggaaccg cgggcgtatt ccgaagatgg ccgaaaccat cgaccggctg atggtgattt
     9361 ttccgttcga ggtcgacgtg tttaaaggcg ttgacctcaa ggtcgacttt gtcggccacc
     9421 cgatggttga cgaactgcgc gaattccgcg agaaagcggc ggagccgctg ccgtggaacg
     9481 ggaaaaagaa aatcgcgctg ctgcccggca gccgcaaaca ggaaattatt tatattctgc
     9541 cgcgcctgct cgaagcggcg cagctt
//
LOCUS       DAOPZO010000077         9260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_8797_length_9260_cov_8.699402, whole genome shotgun sequence.
ACCESSION   DAOPZO010000077 DAOPZO010000000
VERSION     DAOPZO010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 9260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9260
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8797_length_9260_cov_8.699402"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PKI68_06185"
     CDS             <1..194
                     /locus_tag="PKI68_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53315.1"
                     /translation="SRNSGFRRFQHLLKRPDFSRKFWLTILGTLGLVLALLIVWDLFF
                     RYPKLQPDRKQGVYRAMVE"
     gene            complement(199..966)
                     /locus_tag="PKI68_06190"
     CDS             complement(199..966)
                     /locus_tag="PKI68_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNX53316.1"
                     /translation="MIKRSYLISRVMGIPVRVHITLIILLIILTFSSGVFGMLYAVAL
                     FASVALHELGHSWVAIRKGCRVHEIMLLPIGGVAKMNNIPSRPRDEFLIAAAGPLTSF
                     LLAVFFGWLGQFAFFFISLRNVNLMLCLFNLLPSFPMDGGRIFRAFMTPRLGRLKATA
                     LAARIGRIMAVAFAVFGLFQGNFLLIIIAIFIYQSAGAEYRAIYLQQMSQDWFTAGQT
                     ADVEVSPPPYARKAPPVWKKWKNKTNAFFADLFKNWG"
     gene            1122..2114
                     /gene="gap"
                     /locus_tag="PKI68_06195"
     CDS             1122..2114
                     /gene="gap"
                     /locus_tag="PKI68_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNX53317.1"
                     /translation="MIKVGINGFGRIGRMVFRASVGRKDMVVVGINDLLDVNYLAYML
                     KYDSVHGQFEGTVEVKDGKLVVNGNAIRITAEKDPAALKWGEIGAEVVVESTGFFLTK
                     ETAQKHIDAGAKKVVLSAPSKDDTPMFVCGVNTDKYAGQTIVSNASCTTNCLAPVAKV
                     LNDKFGIKRGLMTTVHAATATQKTVDGPSAKDWRGGRGILENIIPSSTGAAKAVGVVI
                     PELKGKLTGMAFRVPTSDVSVVDLTCELVKEASYEEICAAMKEAAAGSLKGILGYTED
                     DVVSTDFRHEARTSVFDAKAGIQLDKTFVKVVSWYDNEWGYSNKVLDLIKVISK"
     gene            2299..2916
                     /locus_tag="PKI68_06200"
     CDS             2299..2916
                     /locus_tag="PKI68_06200"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HNX53318.1"
                     /translation="MRGQKVILDADLAELYGVQTKRLNEQVKRNADRFPEDLMFQLTA
                     QEWENLKSQIVTSNGELLRSQIVTLDKDVDNEPDTPNRSQFATGLSVNKSDKSNRSQF
                     VTGSQRHRDPRFLPRAFTEHGAMMAAMVLNSPEAVAMSLFVIRAFIRMREQLAANAAI
                     LKRLAEIDKTLLEHDQALGLIWEQLQPLLAPPPDPPKRRIGFHQN"
     gene            3170..4426
                     /locus_tag="PKI68_06205"
     CDS             3170..4426
                     /locus_tag="PKI68_06205"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNX53319.1"
                     /translation="MKKMTIRDVDVKGRRVLMRVDFNVPVKDGVVGDVTRITAALPTI
                     NYVLEKGGSLVLMSHLGRPKGKGYEAEFSLKPVAAKLEELLGKPVQFGTDCIGAETIA
                     QAKALKAGEIMVVENTRFYKEEEGKAKLADDATDEQKKAAKAEMKAKQKELAKKMAEL
                     GDGEIFCNDAFGSAHRAHASTAIVCQFYKNNVAGFLMEKEIDYLGKALEAPEKPFVAI
                     IGGAKISGKIDVVTSLMSKCDAILIGGGMAYTFYKAQGFKVGNSLVEDDRVEMAKEIL
                     AQAKAKGVKLMLPIDNIAADKFDVAANTKVVGENIDDGWMALDIGPKTIDLYCAEVAK
                     AKTVVWNGPMGCFEMAPFAKGTFAVCQAVADSDSVSIIGGGDSVSAVNKSGLADKMTH
                     ISTGGGASLEFLEGKQLPGVTALTDK"
     gene            4454..5224
                     /gene="tpiA"
                     /locus_tag="PKI68_06210"
     CDS             4454..5224
                     /gene="tpiA"
                     /locus_tag="PKI68_06210"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNX53320.1"
                     /translation="MRKKIIAGNWKMNKTVADALALANGIKGELSGCGCCSCSCPEVV
                     VCPPFTALKTVGDALAGSKVQLGCQNMSSEDDGAYTGEISHTMLRELGVQYVILGHSE
                     RREYYKETDFWVNKKVKKALEKNLRPIVCVGEKLADREAGNTEKVVEEQVRGSLAGIT
                     AEQFENVVVAYEPVWAIGTGKTATSEQAQEVHAFIRGIIRDMVGEKAAQGLRIQYGGS
                     MKASNAKELLAQPDIDGGLIGGAALEVRSFIDIVKAAV"
     gene            5299..5652
                     /gene="secG"
                     /locus_tag="PKI68_06215"
     CDS             5299..5652
                     /gene="secG"
                     /locus_tag="PKI68_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNX53321.1"
                     /translation="MAVFRTLLIILEAACSLSLIGLILLQKSKNEGLGLAFGSGGGDS
                     LFGARAGNVLTKATVVIGCIFLVNTLVLGMLFAGSASESLLDKASVAQPVPMEQPVAP
                     QQEIPAMPAAAVPAE"
     gene            5670..7499
                     /locus_tag="PKI68_06220"
     CDS             5670..7499
                     /locus_tag="PKI68_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNX53322.1"
                     /translation="MERRNKGRRIVLRRFAVFLCLLLTACGRRDLDTLRFEGEQVLYG
                     QTPRIKGFDPARAGDVPSSVAIGRIYEGLLEYSYLDRPYRVQPLLAAALPDVSEDGLL
                     YTFRLRSGIYFQDDPCFPGGKGRELTAEDFIYSIKRVADLKNGSSGYWAFNGRIAGLD
                     EFRAASAGSAPTDYAAPVEGLRAPDRYTLQIRLKEPYPQLLWVLAMHYSFAVPREAVE
                     FYGENFVNHPVGTGPYILAKWKSNYRIEFIRNPKWAETGRVELYPSEGPEFARAAGLL
                     EDAGKPLPFIDRIVQYVVDDPATTWMMFLTGQFSYSDISRDNWDAVITPSRDLVGKMK
                     EQRIELLAGPTLDLYYIGFNMDDPVVGKNKKLRQALSCAFNPELLVQYYNGRITPVYG
                     PLPDPLAGFKSAPTAYSYNPEKAKKLLAEAGYPDGINPATGRRLELTLELGGATPDVR
                     QVVELMAGMFDKVGIVLKASYNNWPAFLGKMDRRQAQLFQLGWVADYPDAENFLQLFY
                     SANVSPGPNHSNYVNAEFDQLYRQLRTMLPGPEKDRLCEQMADLIIEDCPWVFMYQPM
                     EFAVLHDWLKNYSLHDFPYTLAKYRRVDNAALNRWKAQQGRRD"
     gene            7499..8773
                     /locus_tag="PKI68_06225"
     CDS             7499..8773
                     /locus_tag="PKI68_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX53323.1"
                     /translation="MFRYIAKRILQMISTVFGVILITFILFNAVGGSPALQVLGRHVS
                     PQALEEFDEQRGFNKPLFIGTLTTTRAYADSDFERTAGDWQGISGVTFTNGTITLAAG
                     GEYALPLKFALRTDCKYELELEARSAGFPMFGEQQLKLSKEWKTIRLRFETPGKILPG
                     TAEVEIRSIKLRRVMNSPFDSQFIFYLKQLAHLDFGVSSSTNQRVSKLLLDGIGPSLM
                     LTVPIFFAGLVAAVMLSLFCAFWRDTWIDRFFVVFSVALMSVNYLVWIVGGQYLLGFK
                     LGWFPVWGFEDPRYLILPVLIGVFSGLGAELRFYRTIMLDEMHRDYVRTARAKGVSRG
                     GVLFKHVLKNALIPIITNTVIAIPFLYTGSLLLESFFGIPGLGYLSVNAINSSDVDVV
                     RAVVLVGSILFVVANLLTDLCYALVDPRVKLK"
     gene            8804..>9260
                     /locus_tag="PKI68_06230"
     CDS             8804..>9260
                     /locus_tag="PKI68_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53324.1"
                     /translation="MDKPNIEKGQSLWSDAWRRLKKNRLAMICLGIVGLYTLAALYGE
                     AVYQYYQAKDQTAFYQQTDLSQAYQPPSPSHWMGTDALGRDVTQRLIQGTRIAYKVGI
                     ITALIAIPIGVFFGCLAGYFGGRVDSFVVWLYSTVASMPGLLFILAIAMV"
BASE COUNT         2047 a         2603 c         2659 g         1951 t
ORIGIN      
        1 gcagccgcaa cagcggattc cgccggtttc agcatctgtt aaagagaccg gatttcagcc
       61 ggaagttctg gctgacgatt cttggtacgc tcggactggt gctggctctg ctgattgtct
      121 gggatctgtt tttccgctat ccgaaacttc agcctgatcg caaacagggt gtttaccgcg
      181 ctatggtgga gtaactgcct atccccagtt tttgaacagg tcggcgaaaa aggcgttggt
      241 tttgtttttc catttcttcc agaccggcgg cgctttgcgc gcgtagggcg gcgggctcac
      301 ttccacatcg gcggtctgtc cggcggtgaa ccagtcctga ctcatctgct gtaaatagat
      361 cgcgcggtat tccgcaccgg ccgactggta gatgaaaatg gcgattatga tgagcaagaa
      421 gtttccttga aacaggccga aaaccgcgaa ggcaacagcc ataatacgtc cgatgcgcgc
      481 ggccagcgcg gtggctttaa ggcgtccgag gcgcggtgtc atgaaagcac ggaaaatgcg
      541 tccgccgtcc atcgggaatg acggcagcag gttgaacagg cacagcatca gattgacgtt
      601 gcgcagggaa ataaagaaga aagcgaactg tccgagccag ccgaagaaaa cggccaggag
      661 aaagctggtc agcggcccgg cggcggcgat cagaaactca tctctcggcc gcgaaggaat
      721 attgttcatt ttggcgacgc cgccgatcgg cagcagcatg atttcgtgta cgcggcagcc
      781 tttgcggatg gcgacccatg agtggccgag ttcatgcaaa gcaacgctgg cgaaaagtgc
      841 gacggcataa agcatgccga aaacgccgga cgaaaaggtc agaataatca gaagaataat
      901 cagcgtaatg tgcacccgca ccgggatgcc catcacgcgg gaaatcagat aagagcgttt
      961 aatcatacaa atcctttatg gagcagagtg ttgaggcttt aatcgggggt gtcaagtttg
     1021 cgggtgtgaa ggattgacgc tctgggggtg ttccggtagg gttcgcggga ttttatagaa
     1081 aacagcccct tgacgggggc gcaacgcaaa gggagagaca aatgatcaag gttggtatta
     1141 acgggttcgg acgcatcggc cgcatggttt tccgcgcttc cgtcggtcgc aaagatatgg
     1201 tcgtcgtcgg cattaacgac ctgctggacg tcaactatct ggcgtacatg ctcaaatatg
     1261 attcagtcca cggccagttc gaaggcactg tggaagtcaa agacggcaag ctcgtcgtta
     1321 acggcaacgc aatccgcatc accgcagaaa aagatccggc cgctctcaag tggggcgaaa
     1381 tcggcgctga agttgtcgtc gaatccaccg gcttcttcct gaccaaagaa accgctcaga
     1441 agcacatcga cgccggcgcg aagaaagttg tcctttccgc tccgtccaaa gacgacactc
     1501 cgatgttcgt ctgcggcgtc aacaccgaca aatatgccgg tcagacgatc gtctccaatg
     1561 cttcctgcac caccaactgt ctggctccgg tcgccaaggt tctgaacgac aagttcggca
     1621 tcaaacgcgg tctgatgacc acggttcacg ctgcaaccgc cactcagaaa accgttgacg
     1681 gtccttccgc caaagactgg cgcggcggcc gcggtattct cgaaaacatc atcccgtcct
     1741 cgaccggcgc tgctaaagcg gtcggcgtgg tgattccgga actcaaaggc aagctcaccg
     1801 gcatggcctt ccgcgttccg acctccgacg tatcggttgt cgacctgacc tgcgaactgg
     1861 tcaaggaagc ttcctacgaa gaaatctgcg cggcgatgaa agaagctgcc gccggttccc
     1921 tcaagggcat cctcggctac accgaagacg atgtggtttc caccgacttc cgtcacgaag
     1981 cccgcacctc ggtcttcgat gccaaagccg gtatccagct cgacaagacc ttcgtcaaag
     2041 tcgtttcctg gtacgacaat gaatggggct actccaataa agtcctcgac ctgatcaaag
     2101 ttatcagcaa gtaagctgat cttgattcag ttcaaaagcc gctccggttt ccggggcggc
     2161 ttttttattt tccggttaag aactcgcttc caaaggtctg agtaatatta tacaaacttg
     2221 gaaagatttt gaaatcatgc tgtcggaata ggagatgatg tgaacgagcc aactcgagta
     2281 gagccgttga ttctgacgtt gcgcggccag aaggtgattc tggatgccga tctggctgaa
     2341 ctgtatggtg ttcagaccaa gcggcttaac gagcaggtta aacgtaatgc tgaccgtttc
     2401 cctgaagatt taatgttcca gctgaccgct caggaatggg aaaacttgaa gtcgcaaatt
     2461 gtgacttcaa acggggagct tctaaggtcg caaattgtga ccttagacaa agatgttgat
     2521 aatgaaccgg atacgccgaa ccggtcgcaa tttgcgaccg gtctatccgt aaataaatca
     2581 gacaagtcga accggtcaca atttgtgacc ggttcccagc gtcaccgcga tccgcgattt
     2641 ttgccacggg ctttcaccga acacggcgcg atgatggcgg cgatggtgct gaacagtccg
     2701 gaagcggtgg caatgagcct gtttgtgata cgggctttta tccggatgcg tgaacagctt
     2761 gccgccaatg cggcgatttt gaaacggctg gcggaaattg ataaaacact tctggaacac
     2821 gatcaggcgc tcggattgat ctgggagcag cttcaacccc tgctggctcc accgcctgat
     2881 ccgccgaaaa gacgaatcgg ctttcatcag aactgacgct tcggatttta tcaaaagcat
     2941 ctgtctaaaa acggtttggg tggtcatttt cgactttgtg cggcaagggc atcacgttaa
     3001 aagtcagagg actctaaaat cacggctcag cttgaaaatt gacctgtaat gcagacgctg
     3061 cgaagcatca tccccgcagt tctaaaaacg gggtcggagt catgattgac tccggctctt
     3121 ttttccttat attcgacggg attttttaaa ccttgaaagg ggagctgcca tgaaaaaaat
     3181 gacgattcgt gatgtggatg taaagggccg ccgggtcctg atgcgcgtag atttcaacgt
     3241 gccggtgaaa gacggtgtcg tgggcgatgt aacccgtatt accgcagcgc tgccgacgat
     3301 caactatgtg ctcgaaaagg gcggctcgct cgtgctgatg agccacctcg gccgtccgaa
     3361 gggcaaaggc tacgaagcgg aattcagtct gaagccggtc gccgccaagc tcgaagagct
     3421 gctcggcaag ccggtccagt tcggcaccga ctgcatcggc gccgaaacga ttgcgcaggc
     3481 caaagccctc aaggccggcg aaatcatggt ggtcgaaaac acccgtttct ataaagaaga
     3541 agaaggcaag gccaagctgg ccgacgacgc caccgacgag cagaagaaag ccgccaaggc
     3601 cgagatgaaa gccaagcaga aagagctcgc caaaaagatg gccgaactcg gcgacggcga
     3661 aatcttctgt aacgatgcgt tcggttccgc gcaccgcgct cacgcttcca ccgcgattgt
     3721 ctgccagttc tacaaaaaca acgtcgccgg cttcctgatg gagaaggaaa tcgactatct
     3781 cggcaaggcg ctcgaagctc ccgaaaaacc gttcgtcgcc atcatcggcg gcgccaaaat
     3841 ctccggcaag atcgatgtcg tcaccagcct gatgagcaag tgcgacgcga ttctgatcgg
     3901 cggcggcatg gcctacacct tctacaaagc ccagggcttc aaagtcggca actcgctggt
     3961 cgaagacgac cgcgtcgaaa tggccaaaga gattctcgct caggccaagg ccaaaggcgt
     4021 gaagctgatg ctgccgatcg acaatatcgc cgccgacaag ttcgacgtgg ccgccaacac
     4081 gaaagtggtt ggtgaaaata tcgatgacgg ctggatggca ttggatatcg gcccgaagac
     4141 gattgatctc tattgtgccg aagtggccaa agccaaaacg gttgtctgga acggtccgat
     4201 gggctgtttc gaaatggctc cgttcgccaa aggcactttc gcggtttgcc aggctgtggc
     4261 cgattccgac agcgtcagca ttatcggcgg cggcgacagc gtgagcgctg tcaacaagag
     4321 cggtctggcc gacaagatga ctcacatcag caccggcggc ggcgcgagcc tcgaatttct
     4381 cgaaggcaag cagcttcccg gcgttaccgc gctgaccgat aaataacctt ataaaaacaa
     4441 actggagaat ctcatgagaa agaaaattat tgccggtaac tggaaaatga acaagaccgt
     4501 cgccgacgcg ctggcgctgg caaacggaat taaaggcgag ctgtccggct gcggctgctg
     4561 cagctgctcg tgtcctgaag ttgtcgtttg tcctccgttc acagcattga agacggtggg
     4621 cgatgcgctg gcgggctcca aggttcagct gggctgccag aacatgagct cggaagacga
     4681 cggcgcttac accggcgaaa tttcgcacac gatgcttcgc gagctgggcg tgcagtacgt
     4741 cattctcggt cacagcgaac gccgcgagta ctacaaagaa accgacttct gggtgaacaa
     4801 gaaggtcaaa aaggcgctcg aaaaaaatct gcgcccgatc gtttgcgtcg gtgaaaaact
     4861 ggccgaccgc gaagccggca acaccgagaa ggtggtcgaa gagcaggtgc gcggcagcct
     4921 ggccggcatt acggccgagc agttcgaaaa cgttgttgtt gcctacgaac cggtctgggc
     4981 gatcggaacc ggcaagacgg cgaccagcga acaggctcag gaagtgcatg cgttcatccg
     5041 cggcatcatc cgcgacatgg tcggcgaaaa agcggcccag ggcctgcgca ttcagtacgg
     5101 cggaagcatg aaagcgtcga atgccaagga actgctcgcg cagcctgaca ttgacggcgg
     5161 actgattggc ggcgcagctc tcgaagtccg ctcgttcatc gatatcgtca aagcggcggt
     5221 gtaatccggg ccgggagcgg aatttgtact cgcaattccg ctcccatagc ctaaagttcg
     5281 aggcctctgg aggtaaaaat ggctgttttt cgtacattac tgattattct tgaagccgca
     5341 tgcagtttgt ccctgatcgg tttgattctg ctgcagaaat caaaaaatga agggctcggt
     5401 ctggcgtttg gttcgggcgg cggcgactcg cttttcggcg cgcgtgccgg caacgtactc
     5461 accaaagcta cagtcgtgat cgggtgcata ttcctggtga acacgctggt tctcggtatg
     5521 cttttcgccg gcagcgccag cgaatcgctt ctggataaag cttctgttgc acagcctgtg
     5581 ccgatggagc agcctgttgc gccgcagcag gaaatccctg caatgccggc tgctgctgtt
     5641 cccgctgagt aataccggga gcgcgattca tggaacggcg aaacaagggc aggagaattg
     5701 tactccgtcg tttcgccgtt tttctttgtc tgctgctgac ggcctgcggt cggcgcgatc
     5761 ttgacacctt gcgttttgaa ggcgagcagg tgctctacgg ccagacaccc cgcattaaag
     5821 gtttcgatcc ggcccgcgcc ggcgatgttc cctcgtcggt cgccatcggg cggatttacg
     5881 aaggactgct cgaatattcc tatcttgacc ggccgtaccg cgtccagccg ctgctcgccg
     5941 ccgcgctgcc cgacgtttct gaggacggcc tcctgtatac cttccggctc cgctccggca
     6001 tttattttca ggacgatccc tgctttcccg gcggaaaagg ccgcgagctg acggcggagg
     6061 atttcatcta ctccatcaaa cgcgtcgccg atctgaaaaa cggctcgtcc ggctactggg
     6121 ccttcaacgg gcgcattgcc ggcctcgacg aattccgcgc cgcgtcggcg ggcagtgcgc
     6181 cgacagacta tgccgcgccg gtcgaagggc tgcgcgcccc ggatcgctac accttgcaga
     6241 tccggctcaa agaaccctat ccgcagcttc tctgggtgct cgcgatgcac tacagctttg
     6301 cggtgccgcg cgaagcggtt gagttttacg gcgaaaattt cgtcaatcat cccgtcggca
     6361 ccggccccta tattctggcg aagtggaaga gcaattaccg catcgaattc atccgcaatc
     6421 cgaagtgggc cgagaccgga cgggtggagc tctatccctc cgaagggccc gaattcgccc
     6481 gcgccgccgg actgctcgag gacgccggca agccgctgcc gtttatcgac cgcattgttc
     6541 aatacgttgt ggatgatccg gcgacgacct ggatgatgtt tctgaccggg cagttcagct
     6601 acagcgatat ttcgcgcgac aactgggatg cggtcatcac gcccagccgc gatctggtcg
     6661 gcaagatgaa agagcagagg attgagctgc tggccggtcc gacgctcgat ctgtattaca
     6721 tcggcttcaa catggacgat ccggtcgtcg gcaaaaataa aaaactgcgt caggcgctct
     6781 cctgcgcctt caatccggag ctgctggtgc agtattacaa cggacgcatc acgccggttt
     6841 acggcccgct gcccgatccg ctggccggat tcaaatccgc accgacggcc tattcctata
     6901 atcccgaaaa agccaaaaaa ctgctggccg aagccggtta tccggacggc atcaatccgg
     6961 ccaccggacg gcgccttgag ctgacgctgg agctcggcgg cgcgacgcct gatgtgcggc
     7021 aggtcgtgga actgatggcg ggcatgttcg ataaagtcgg cattgtgctg aaggccagct
     7081 acaacaactg gccggctttc ctcggaaaaa tggaccgccg gcaggcccag ctcttccagc
     7141 tcggctgggt cgccgactat cccgacgccg aaaactttct gcagctgttt tacagcgcca
     7201 acgtttcgcc cggccccaac cattccaact atgtcaacgc ggagttcgac cagctctatc
     7261 ggcagctgcg cacgatgctg cccggcccgg aaaaagaccg gctttgtgag cagatggccg
     7321 acctgattat cgaagactgc ccctgggtct tcatgtacca gccgatggag tttgccgttc
     7381 tgcacgactg gctgaaaaac tacagcctgc acgactttcc ctacaccttg gcgaaatacc
     7441 gccgcgtcga taacgccgct ctgaaccggt ggaaagcgca gcaggggagg cgcgactgat
     7501 gttccggtat atcgccaaac gcattctgca aatgatctcg accgtgttcg gcgtcatcct
     7561 gattacgttt attctgttta acgcggtcgg cggcagcccg gcgctgcagg tgctgggccg
     7621 gcatgtgtcg ccgcaggcgc tcgaagagtt cgatgaacag cgcggcttta acaaaccgct
     7681 cttcatcggg acgctcacga caacccgcgc ctatgccgac tccgactttg aacgcacggc
     7741 gggtgactgg cagggcatca gcggcgtgac cttcacgaac ggcacgatca ctctggccgc
     7801 gggcggcgaa tatgcgcttc cgctcaagtt cgcgctgcgc accgactgca aatacgaact
     7861 ggagctggag gcccgttccg ccgggttccc gatgtttggc gagcagcagc tcaagctttc
     7921 caaagagtgg aaaaccatcc ggctgcggtt cgagacgccg ggaaaaattc tgccgggcac
     7981 tgccgaggtt gaaatccgtt cgatcaaact gcgccgcgtg atgaactcgc cgtttgattc
     8041 gcagttcata ttttatctca agcagctcgc gcatctcgac ttcggcgttt cgagcagcac
     8101 caatcagcgc gtctcgaagc tgctgctcga cggcatcggg ccgtcgctga tgctcacggt
     8161 cccgatcttt tttgccggac tcgtcgccgc ggtcatgctc tctcttttct gcgccttctg
     8221 gcgcgacacc tggatcgacc ggttcttcgt cgtcttttct gtggcgctga tgagcgtcaa
     8281 ctatctggtc tggatcgtcg gcgggcagta tctgctcggc tttaaactcg gctggtttcc
     8341 ggtctgggga ttcgaagacc cgcgctacct gattctgccg gtgctgatcg gcgttttcag
     8401 cggtctcggt gccgaactgc gcttctaccg caccatcatg ctggatgaaa tgcatcgcga
     8461 ctacgtccgc acggcacggg ccaaaggcgt cagccgcggc ggcgtgctgt tcaagcacgt
     8521 tttgaagaac gctttgattc cgatcatcac caacaccgtc atcgccatcc cgtttcttta
     8581 taccggcagc ctgctgctgg agagcttctt cggcattccg ggtctcggct atctcagcgt
     8641 taacgccatc aactcgtccg atgtcgatgt cgtccgcgcg gtcgtgctgg tcggctcgat
     8701 cctgtttgtt gtcgcgaatc tcttaaccga cctctgctat gcactggtcg atccgcgggt
     8761 gaaactgaag tgaaatgtga aacctgaaac tggaaacttg aaattggata agccgaatat
     8821 agaaaaaggg caatcgctct ggagtgatgc ctggcggcgg ttgaagaaga accggctggc
     8881 gatgatctgt ctgggcatcg tcggcctcta tacgctggcc gcgctgtacg gcgaagcggt
     8941 ctaccaatat tatcaggcca aagatcagac cgcattttac cagcagaccg atctcagtca
     9001 ggcctatcag ccgccgtctc ccagccattg gatgggcacc gacgcgctgg gccgcgatgt
     9061 gacgcaacgg ctgattcagg gcacgcgcat tgcctataaa gtcggcatca tcaccgcgct
     9121 gatcgccatt ccgattggcg ttttcttcgg ctgtctggcg ggctacttcg gcgggcgcgt
     9181 ggacagcttt gtcgtctggc tctactccac cgtcgcttcc atgccgggcc tgctcttcat
     9241 tctcgccatc gccatggtgg
//
LOCUS       DAOPZO010000078         9188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_8889_length_9188_cov_9.657944, whole genome shotgun sequence.
ACCESSION   DAOPZO010000078 DAOPZO010000000
VERSION     DAOPZO010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8889_length_9188_cov_9.657944"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="PKI68_06235"
     CDS             complement(<1..1304)
                     /locus_tag="PKI68_06235"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX53325.1"
                     /translation="MCYALPGVVTEISGRNAVISYFGEIRSALCELTGVQPGDYVYAQ
                     GGYVIEKVDPDFAREMLDTWNELFKVLQERDANMSARPTPEPSDRPLQDILDKAGAGE
                     SISRDELRYLLTVEEPERLERLYRTANAVRHLHHRNSCCVHGILEISNYCSSDCLYCG
                     LAANSTLTRRYRMTRDEILEAAKEAIEGFGFKSLVLQSGEDPAYTPAFLAEIVAEIKR
                     RHGVFICVSFGEVGLDGLDMLYEAGARALLMRFETSDAKLYAALHPGDTLDARLSHLR
                     HALKTGWLTVTGGLIGLPGQTVDSLADDILLAGELNAEMFSFGPVLASHRRRDIIPAT
                     EPQMLRALAAARIAAPSQAKVLVTTAFETLSPNARERGLMAGASSVMLNVTPLRYRAD
                     YAIYDGRAHADESIADQTRAIIQLLDRLGRAPSDLGVATPSLS"
     gene            complement(1304..1648)
                     /locus_tag="PKI68_06240"
     CDS             complement(1304..1648)
                     /locus_tag="PKI68_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase/urease maturation nickel
                     metallochaperone HypA"
                     /protein_id="HNX53326.1"
                     /translation="MHEDHLIDGVIEDIKRRLSAAGASRVSRIVLELSPDSHMDEISV
                     EMHLESHLAEEPAMADAKIEYRHVPPRLFCPACNAEFERQPGVFTCPQCSKPGRPCGH
                     RSGLRVLDVDLV"
     gene            complement(1650..1964)
                     /locus_tag="PKI68_06245"
     CDS             complement(1650..1964)
                     /locus_tag="PKI68_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNX53327.1"
                     /translation="MKKMILAWTFRTPVTTKYPFQPRKICPGSRGRLDIKITDCIFCT
                     LCAKRCPTQALEVDRTNKKWHIDRLRCISCGACVEVCPKKCLALTGDHGIPAVTKDME
                     TF"
     gene            complement(1984..3063)
                     /locus_tag="PKI68_06250"
     CDS             complement(1984..3063)
                     /locus_tag="PKI68_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNX53328.1"
                     /translation="MGNRTIIPFGPQHPVLPEPIHIDLVVEDEKVVEAVPSIGFIHRG
                     LEKLTEKKDFIEYVYVAERICGICSFIHGQTYCQGIEEIMKIEIPSRAKYLRTIWGEL
                     SRIHSHLLWLGLMADAFGFESLFMHSWRVREEVLNLIEATTGGRVIFGSCKVGGTRRD
                     IPPEQMTWLLAELTKIEKEMRLIVDIFLNDNSVKHRLCGVGVISKDDAIALGAVGPTL
                     RASGVADDMRLLKYAAFGELNFQPIVRNEGDSYGRCAVRCEELFQSVDLIRQAAAKMP
                     AGEIAVKVTGNPDGEFFSRVEQPRGEVVYYIKGNGTKNLTRFRARTPTFANVPPLVKM
                     LAGCELADVPVLVLTIDPCISCTER"
     gene            complement(3066..3458)
                     /locus_tag="PKI68_06255"
     CDS             complement(3066..3458)
                     /locus_tag="PKI68_06255"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNX53329.1"
                     /translation="MIEEQTIIAVMPETLVAETRKMKEQGYRLVQICVTRLGEELQLD
                     YTFDLNRKFVNFRFLQPKNGARVPSISSLYWCAFTYENEISDLFGVQVDGNALDFKGT
                     FYKTAMKHPFIQDAAPAVVPAPAKAGGQ"
     gene            complement(3455..4360)
                     /gene="nuoB"
                     /locus_tag="PKI68_06260"
     CDS             complement(3455..4360)
                     /gene="nuoB"
                     /locus_tag="PKI68_06260"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HNX53330.1"
                     /translation="MTAFKKSPWVIHYDASSCNGCDIEVLACLMPNFDVERFGIINTG
                     NPKHADIFLITGSVNVQNAPVVRNIYAQMPEPKVVVAIGICATSGGIFRECYNVMGGI
                     DTVIPVDVYVPGCAARPEAIIDGVVRGLGVLEEKRRFMKSSAEAAAGEVLINRANLKD
                     VPEILALQKIAYRSEAELYGDTSVPALSQTIEEISADFDRMIFLKAIVNGKIIGSVRA
                     RLDGDTAHISRVIVHPYFQGKGLATRLIQQIEQEVGDVRAYEAFTGHQSVQNLHLYGK
                     LGYRQVRTEPFTSTVNWVYLQKERT"
     gene            complement(4374..7223)
                     /locus_tag="PKI68_06265"
     CDS             complement(4374..7223)
                     /locus_tag="PKI68_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNX53331.1"
                     /translation="MIENLIPWMVAFPLLAGVVLFFISNNVLRRYAVYAATAAIMIGS
                     VLLAWKSNQNTMLLDGSAHNISLAMLAIETLIVGFIVWVTVRAKNWLALGLVLVQFIL
                     VAGFELQEMTKAPSAAESVGSAIATAVSTVSPWLLCDRLSVIMVLVIGIVGGLICIYA
                     PGYMTEFHQQHPEFRDRRPMFFATFFIFLGAMFGIVFSNSLNWLYFFWEITTLSSFLL
                     IGYKETDESKTNAMRALTMNLMGGVGFALAIVLLGNHEISSLMDLRGQGNTSQVIVIA
                     AGLLAFAGITKAAQFPFAGWLRGAMVAPTPVSALLHSSTMVKAGVYLVLRLAPNLEGT
                     KVGMMVALVGAVTFVAGSLVAICTSDAKKILAYSTVANLGLIVLCGGIGTKEAVWAGI
                     LLIIFHAVAKALLFLCVGVVEHKTHSRDIEDMAGLVLRMPKVAVMILIGIAGMFLAPF
                     GMLISKWAVLKAIVDAHPILSIFIVFGSSATLFFWVKWMGKMLQITGPMDKKEKNIGG
                     GEWIALGSLAVFTILTCMTFPLISNQFIEPYIGGGFHAADSVLGTSNLIVMGLMLCMV
                     AMFPLSFLNYNRKVKVVDAYLAGANVESTDGQSRFQGSAGTIRDVSMHNYYGSQLLGE
                     ERVFKAGFWIAIAVLTALAGVFLLPVGLPQEVIHSIAPRALPLKEFAIRLAAYLVLAP
                     VIGGFLAGIDRKLTARMQGRRGPPIRQAFYDVAKLWGKENIVVRRSQSFYVFFFFLFV
                     VFTGALFFCGSDLLLVIFSLTLASIFLVLAAYKASSPYSFIGAERELIQMVAYEPMVL
                     MAAVGMYMTTGSFRVDDIFANPANPLKLILIFAGFLYILEIKFRKSPFDLSTSHHAHQ
                     ELVKGLTTEFSGKMLALIEVAHWYENVILFAWVGLFFAFNLWIGAAVMVLVFFFMIFV
                     DNTFARLKWQLAIKSAWTVTLVLAVANLILLALWK"
     gene            7481..>9188
                     /locus_tag="PKI68_06270"
     CDS             7481..>9188
                     /locus_tag="PKI68_06270"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR00178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNX53332.1"
                     /translation="MNNKAKILYTITDEAPALATQSLLPIIEAFTSAAGVAVEPRDIS
                     LAGRILANFPDFLTAAQRVPDALAELGELTLKPEANIIKLPNISASLPQLKACIAELQ
                     AQGFKLPDYPETPATAEEKEIKARYDKIKGSAVNPVLREGNSDRRCAAAVKQYAKNNP
                     HRMGAWSPDSKSVVSSMPGNDFFGNEKSVTVPAATVARIELIAADGSVTVLKDGLKLQ
                     AGEVLDGTFLSVRSLREFFSGQIAATKKAGTLFSLHMKATMMKVSDPIIFGHCVSVFF
                     KDVFEKHAATFAKLGVSPNNGLGDLYAKIETLPADEKTAIEADIAAVYQSAPDIAMVD
                     SDKGITNLHVPSDIIVDASMPAAIRASGQMWNKDGKQQDTNFIIPDRCYSGIYAETIA
                     FCKQHGAFDPRTMGSVANVGLMAQQAEEYGSHNKTFEIPAAGTVRVTDAAGNVLFEHK
                     VEKGDIWRACQVKDAPIRDWVKLAVTRARATGTPAIFWLDQNRAHDAQLIAKVEKYLQ
                     EHDTAGLDICILPPVEATRRSLERAKAGLDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSI"
BASE COUNT         2035 a         2696 c         2717 g         1740 t
ORIGIN      
        1 gagagcgagg gtgtggcgac gccgaggtcg ctgggagcgc ggccgaggcg gtcgaggagc
       61 tggatgatgg cgcgggtctg gtcggcgata ctttcgtcag cgtgggcgcg tccgtcgtag
      121 atggcgtagt cggcgcggta acgcagcggc gtaacattca acatgaccga gctggcaccg
      181 gccatgagtc cgcgttcgcg ggcattcggc gacaaagttt caaaagcggt ggtgaccagc
      241 actttggcct gcgacggcgc ggcgatccgg gcggcggcca gcgcgcgaag catctgcggt
      301 tcggtggccg gaataatgtc gcggcggcga tgactcgcca gtaccgggcc gaagctgaac
      361 atttcggcat tcagttcacc ggccagcaga atatcgtcgg ccagcgagtc aacggtctgg
      421 ccgggcagac cgatcagccc gccggtcacc gtcaaccagc cggtcttgag cgcgtggcgc
      481 agatgactca ggcgggcatc cagcgtatcg ccgggatgga gcgcggcata cagttttgcg
      541 tccgacgttt caaagcgcat cagcaaggcg cgggcgccgg cttcgtacag catgtcgagg
      601 ccgtcgaggc cgacttcgcc gaagctgacg cagatgaaga cgccgtggcg gcgtttaatc
      661 tcggcgacga tttcagccag aaacgccgga gtgtaagccg gatcttcgcc ggactggagt
      721 accagcgatt taaagccgaa cccttcaatc gcttccttgg ccgcttcgag aatttcgtcg
      781 cgggtcatcc ggtagcgccg ggtcagcgtg gagttggccg ccagcccgca gtagaggcag
      841 tcgctggagc agtagtttga aatttcgaga atgccgtgca cgcagcagga attgcggtgg
      901 tgcagatggc ggacggcatt ggcggtgcgg tagagccgtt caaggcgctc cggctcttcg
      961 acggtcagca gatagcgcag ttcgtcgcgg gaaatagatt cgccggctcc ggctttatcg
     1021 agaatgtcct gaagcggacg gtcactcggt tccggcgttg gccgcgcgga catattggcg
     1081 tcgcgttcct gcagtacttt gaacagctcg ttccaggtat cgagcatttc tcgggcgaag
     1141 tcgggatcga ctttttcgat cacataaccg ccctgcgcat aaacatagtc gccgggctgg
     1201 acgccggtga gttcgcagag tgctgaacgg atttcaccga aatagctgat cactgcattg
     1261 cgaccgctga tctccgtcac aacgcctgga agcgcgtagc acatcagacc agatccacgt
     1321 caagcacccg caggccggaa cggtgtccgc agggacggcc gggcttggag cattgcgggc
     1381 aggtaaagac gccgggctgg cgttcaaatt cggcattgca ggccggacag aaaagacgcg
     1441 gcggcacatg gcggtattca atttttgcat cggccatcgc cggctcttcg gccagatgcg
     1501 attcgagatg catttccacc gagatttcat ccatgtggga atcggggctc agctcaagaa
     1561 cgatacggct tacccgcgac gctcccgccg cggaaagccg ccgcttgata tcttcgatga
     1621 caccgtcaat gagatgatct tcatgcatct cagaatgtct ccatatcctt cgtaactgcc
     1681 ggaatgccgt gatcgccggt cagcgccagg catttcttcg ggcagacttc gacgcaggcg
     1741 ccgcaggaga tgcagcgcag acggtcgatg tgccatttct tgtttgtccg gtcgacttcc
     1801 agcgcctggg tcgggcagcg cttggcgcac agggtgcaga agatgcaatc ggtgatcttg
     1861 atgtccagac gcccgcgcga accgggacag attttccgcg gctggaacgg gtatttggtc
     1921 gtaaccggcg tgcggaatgt ccacgccaga atcatttttt tcatttggaa gaatgccatg
     1981 gtattacctc tccgtgcagc tgatgcaagg gtcaatcgtc aggacgagaa ccggcacatc
     2041 ggccagttcg cagccggcca gcattttcac cagcggagga acgttggcga aagtcggcgt
     2101 acgggcgcgg aagcgggtca ggttcttggt gccgttgccc ttgatgtaat aaaccacttc
     2161 gccgcggggc tgttcgacgc gcgagaagaa ctcgccgtcc ggattgccgg tcactttgac
     2221 ggcgatttcg ccggccggca tcttcgccgc cgcctgacgg atcaggtcga ccgactggaa
     2281 gagctcttcg caacggaccg cgcagcggcc gtaggagtcg ccttcgttcc ggacgatcgg
     2341 ctggaagttc agttcgccga acgcggcgta cttcagcaga cgcatatcgt cggccacgcc
     2401 gctggcgcgc agggtcggac cgaccgcgcc gagggcgatc gcgtcgtctt tggagatgac
     2461 gccgacgccg cagaggcggt gtttgacgga gttgtcgttg aggaaaatgt cgacgatcag
     2521 ccgcatttct ttttcgattt tcgtcagctc ggccagcagc caggtcatct gttccggcgg
     2581 aatgtcgcgg cgggtgccgc cgactttgca ggaaccgaaa atgacgcggc cgccggtggt
     2641 cgcttcaatc aggttaagaa cctcttcgcg gacgcgccag ctgtgcatga aaagcgattc
     2701 gaagccgaat gcgtcggcca tcagaccgag ccacagcaga tggctgtgga tacgggaaag
     2761 ttcgccccag atcgtgcgca ggtatttggc gcgcgaagga atctcgatct tcatgatttc
     2821 ttcgatcccc tggcagtagg tctggccgtg gatgaagctg cagattccgc agatgcgttc
     2881 ggcgacgtag acgtattcga tgaagtcctt cttctcggtc agcttttcca gtccgcggtg
     2941 aatgaacccg atggacggaa cggcttcgac tactttttcg tcttcgacca ccaggtcgat
     3001 atgaatcggc tccgggagaa ccgggtgctg cgggccgaaa ggaatgattg tgcgattacc
     3061 catgattatt gacctcccgc tttggccgga gccggaacaa cggccggcgc agcgtcttga
     3121 atgaacggat gtttcatggc ggttttgtag aacgtgcctt tgaaatcgag agcattgccg
     3181 tctacctgaa cgccgaacag atcgctgatc tcgttctcgt aggtgaaagc gcaccagtag
     3241 aggctgctga tgctcggaac gcgggcgccg ttcttcggct gcaggaagcg gaagttgacg
     3301 aacttgcggt tgaggtcaaa ggtgtagtcc agctgaagct cttcgcccag ccgggtgaca
     3361 cagatctgaa cgagccggta gccctgctct ttcattttcc gcgtttcggc caccagcgtt
     3421 tccggcatga cggcgatgat ggtttgttct tcgatcatgt gcgctccttc tgcaaataaa
     3481 cccagttcac cgtcgaggtg aacggttcgg tccggacctg ccggtatccc agcttcccgt
     3541 agaggtgaag attctgcacg ctctggtgcc cggtgaaagc ttcgtatgcg cggacgtcac
     3601 cgacttcctg ttcgatctgc tgaatcagac gggtggccag tcctttgccc tgaaaatacg
     3661 gatgaacaat cacccggctg atgtgggcgg tatcgccgtc gaggcgggcg cggaccgagc
     3721 cgatgatttt tccgttgacg atcgccttga gaaaaatcat ccggtcgaag tcggcggaaa
     3781 tctcttcgat tgtctggctg agcgccggaa cgctggtgtc gccgtaaagt tcggcttcgc
     3841 tgcggtaggc gatcttctgg agtgccagaa tttcggggac atctttcaga ttcgcgcggt
     3901 tgatcaggac ttcgccggcg gcggcttctg cggaggattt catgaaccgg cgtttttctt
     3961 cgagaacacc caggccgcga accacgccgt caatgatcgc ttcggggcgg gccgcgcagc
     4021 cgggcacgta gacgtcgacc gggatcacgg tgtcgatgcc gcccatgacg ttgtaacatt
     4081 cgcggaagat accgccggaa gtggcgcaga taccgatggc gacaaccact ttcggttccg
     4141 gcatctgagc gtagatgttg cggacgaccg gtgcgttctg aacgttgacc gagccggtga
     4201 tcagaaagat gtcggcgtgc ttggggttgc cggtgttgat gatgccgaac cgttcgacgt
     4261 caaagttcgg catcaggcag gcgagcactt cgatgtcgca gccgttacag ctcgacgcat
     4321 cgtaatgaat cacccatgga gattttttaa aggcagtcat gcaggtattc ctttcatttc
     4381 cacagggcca gaagaatcag gttggccacg gcaagcacca gagttaccgt ccaggcgctc
     4441 ttgatggcaa gctgccactt caggcgggca aaggtgttat caacgaaaat cataaagaag
     4501 aagaccagaa ccatgacggc cgcaccgatc cacaggttga aggcgaagaa gaggccgacc
     4561 cacgcgaaca gaatgacgtt ttcgtaccag tgggcaactt cgatcagcgc cagcatcttg
     4621 ccggaaaact cggtcgtaag tcctttcacc agttcctggt gggcatggtg agaggtcgac
     4681 aggtcaaacg gtgatttgcg gaatttgatt tccagaatgt agaggaagcc ggcgaagatc
     4741 aggatcagct tcagcggatt ggccggattg gcgaagatat cgtcaacgcg gaagcttccg
     4801 gtggtcatgt acatgccgac tgcggccatc agcaccatcg gctcgtaggc gaccatctgg
     4861 atcagctcgc gctcggcgcc gatgaagctg tagggcgaac tcgccttgta ggccgcgagg
     4921 acgaggaaga tgctggccag cgtcagcgag aagatcacca gcagcaggtc ggagccgcag
     4981 aagaacaatg cgccggtgaa gacgacgaag aggaagaaga aaaatacgta gaagctctgc
     5041 gagcggcgga ccacgatatt ttctttaccc cagagtttgg cgacatcgta gaaagcctgg
     5101 cggatcggcg ggccgcggcg gccctgcata cgggccgtca gcttgcggtc gataccggcc
     5161 agaaaaccgc cgatgaccgg tgccagcacc agatacgccg ccaggcggat tgcgaattct
     5221 ttgagcggaa gcgcgcgcgg agcgatgctg tggatgactt cctgcgggag accgaccggc
     5281 agcaggaaaa cgccggcaag cgcggtcagc accgcaatcg cgatccagaa gcccgctttg
     5341 aaaacccgtt cttctccgag cagctggctg ccgtagtagt tatgcatcga tacgtcgcgg
     5401 atcgtgccgg cggaaccctg gaagcgggac tgaccgtcgg ttgattcaac attcgcgccg
     5461 gcgagatagg catccacaac ctttaccttg cggttgtagt tgaggaagct gagcgggaac
     5521 attgcgacca tacacagcat caggcccatg acgatcaggt tgctggtgcc cagaacgctg
     5581 tccgccgcat ggaagccgcc gccgatgtac ggttcgataa actggttgga aatcagcggg
     5641 aaggtcatac aggtgagaat ggtgaaaacc gccagcgaac ccagcgcgat ccattcgccg
     5701 ccgccgatgt tcttctcttt cttgtccatc gggccggtga tctgaagcat cttgcccatc
     5761 cacttgaccc agaagaaaag cgtggccgag cttccgaaga cgatgaaaat ggaaaggatc
     5821 ggatgcgcat caacgattgc tttgagaacc gcccatttgc tgatcaacat accgaacggg
     5881 gcgaggaaca tgccggcgat accgatcaga atcatgacgg cgactttggg catgcggaga
     5941 acgagtccgg ccatatcttc gatgtcgcgg ctgtgcgttt tatgttcgac gacaccgacg
     6001 cagagaaaga gcagcgcttt ggccacggca tggaaaataa tcagcaggat tccggcccag
     6061 accgcttctt tggtgccgat accgccgcaa aggacgatca aaccgaggtt ggcaaccgtc
     6121 gagtacgcca gaatcttctt ggcgtcgctc gtgcagatgg cgacgagtga accggcgaca
     6181 aacgtgacgg cgccgaccag agcgaccatc ataccgacct ttgttccttc cagattggga
     6241 gcgaggcgga gaaccagata aacgcccgct ttcaccatcg tgctggagtg aagcagtgcg
     6301 gaaaccggtg tcggagccac catcgcaccg cgcaaccatc cggcaaacgg gaactgggcg
     6361 gctttagtga taccggcaaa tgcgagcagt ccggcggcaa taacgatcac ctgcgaggtg
     6421 ttcccctgac cgcgcagatc catcaggctg ctgatttcat ggttgcccag aagaacaatc
     6481 gccagagcaa agccgacacc gcccatcagg ttcatcgtca gggcgcgcat ggcgttggtt
     6541 ttggactcgt cggtctcttt atagccgatc agcaggaagg aactcagggt ggtgatttcc
     6601 cagaagaaat aaagccagtt caggctgttg gaaaatacga taccgaacat tgcgcccagg
     6661 aagatgaaga acgtggcgaa aaacatcggg cggcggtcgc ggaattccgg atgctgctgg
     6721 tggaattccg tcatatagcc gggcgcatag atgcagatca acccgccgac aatgccgatc
     6781 accagcacca tgatcacgga aagccggtca cagagaagcc agggcgaaac ggtagaaact
     6841 gcggttgcga tggccgagcc gaccgattca gcggcagacg gcgccttggt catctcctgc
     6901 aattcgaacc cggcaaccag aataaactgc accagcacca gacccaaagc cagccagttc
     6961 ttcgcccgca cggtcaccca gacgataaag ccgacgatca gggtttcgat ggccagcatg
     7021 gctagactga tgttatgggc ggagccgtcc agaagcatcg tattctgatt ggacttccac
     7081 gccaaaagga cagatccgat catgatggcg gccgtggcgg cataaaccgc atagcgtcgc
     7141 aggacgttat tggaaatgaa aaaaaggact acgccggcca acaacggaaa agccaccatc
     7201 cagggaatca ggttctcaat cattacctac acctcgttac ttcattccga atcattcgcg
     7261 acggaccccg gcaaggagga tgccgcacgg aaaagcgaag aactataagg attgtccgcc
     7321 gacattcaac aacatcttta cccgaaaatt tccggcaggt tctctcctct ataaaaggtc
     7381 agggaacccg ccttgacttc attccggctt tcaaatacac agagtgcccc ttttcaagga
     7441 cagcagacgg gctgaaggtc aatttaacga aaacaatctt atgaacaata aagccaaaat
     7501 cctctacacg atcaccgacg aagccccggc tctggccacg caatccctgc tgccgatcat
     7561 tgaagccttt acctccgccg ccggcgtagc cgttgaaccc cgcgatattt ccctcgccgg
     7621 ccgcatcctc gccaacttcc cggacttcct gaccgccgcc cagcgcgttc ccgacgcgct
     7681 cgccgaactg ggcgaactga ccctgaagcc cgaagccaac atcatcaaac tgccgaacat
     7741 ttccgcatcc cttccccagc tgaaggcctg tatcgccgaa ctgcaggccc aaggcttcaa
     7801 actccccgac tatcccgaaa cgcccgccac cgccgaagaa aaagagatca aggcgcgcta
     7861 cgacaaaatc aaaggcagcg cggtcaaccc ggtcctgcgc gaaggcaact ccgaccgccg
     7921 ctgcgccgcg gcggtcaaac agtacgccaa aaacaacccg caccgcatgg gcgcgtggtc
     7981 gcccgactcc aaatccgtcg tctccagcat gccgggcaac gatttcttcg ggaacgaaaa
     8041 gtcggtcacc gttccggcgg caaccgtcgc caggattgaa ctcattgcgg ccgacggcag
     8101 cgtaacggtg ctcaaagacg gactgaaact tcaagccggt gaagtactcg acggcacctt
     8161 cctgagcgtc cggtcactgc gcgagttttt ctccggccag atcgccgcga ccaaaaaggc
     8221 aggcacactc ttttccctgc acatgaaggc caccatgatg aaggtctccg acccgatcat
     8281 cttcggccac tgcgtctctg tcttctttaa ggacgtcttt gaaaagcacg ccgcgacgtt
     8341 cgcgaaactc ggcgtctccc cgaacaacgg cctcggcgac ctctacgcca agatcgaaac
     8401 ccttccggct gacgaaaaga ccgccatcga agccgatatc gccgcggtct accagtccgc
     8461 tccggacatc gccatggttg attccgacaa aggcatcacc aacctgcatg tgccgagcga
     8521 catcatcgtc gatgcctcca tgcccgcggc catccgcgcg tccggacaga tgtggaataa
     8581 agacggcaaa cagcaggata cgaacttcat cattcccgac cgctgctact ccggcatcta
     8641 cgccgaaacc atcgccttct gcaaacagca cggcgccttc gatccgcgca cgatgggcag
     8701 cgtagccaac gtcggcctca tggcgcagca ggccgaagaa tacggctcgc acaacaaaac
     8761 cttcgaaatc ccggccgccg gcaccgtccg cgtcaccgac gccgccggca acgtgctgtt
     8821 tgaacataaa gtggaaaaag gcgacatctg gcgcgcctgc caggtgaaag acgcgccgat
     8881 ccgcgactgg gtcaaactcg ccgtcacccg cgcccgcgcc accggcaccc cggccatctt
     8941 ctggctcgat caaaaccgcg cccacgacgc gcagctgatc gccaaagtcg aaaaatacct
     9001 gcaagagcac gacaccgccg ggctcgacat ctgcatcctg ccgccggtcg aagccacgcg
     9061 ccgctccctc gaacgcgcca aagccggact cgacaccatc tccgtcaccg gcaacgtcct
     9121 gcgcgactac ctcaccgacc tcttcccgat tctcgaactc ggcaccagcg ccaagatgct
     9181 ctccatcg
//
LOCUS       DAOPZO010000079         9151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_8945_length_9151_cov_8.032542, whole genome shotgun sequence.
ACCESSION   DAOPZO010000079 DAOPZO010000000
VERSION     DAOPZO010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 9151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9151
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8945_length_9151_cov_8.032542"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /locus_tag="PKI68_06275"
     CDS             complement(<1..1571)
                     /locus_tag="PKI68_06275"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNX53333.1"
                     /translation="MSWSDSLEEGTPAYNIASSTSQNVRVLAGPGTGKSFAMKRRVAR
                     LLENGANPKDILAVTFTRVAAEDLHRELVGMNVPRCSELTGTTLHSLALRILMRNHVL
                     SATGRNPRPLNGFEMAPLEVDLTAHGGLREVRKKIQAYQSAWARLQHDDPGSPINPAD
                     HAFNRDLLSWLIFHNSMLIGEVVPLLYQYLQSNPLAPERTEYGHILVDEYQDLNKAEQ
                     GVIDLLSDNAEVCIVGDDDQSIYSFKYAHPEGIREWIASRPSLADLTLIECRRCPTRV
                     TEMANSLIEHNTNRPTQRQLIPRAENGEGIVELIQFATLQEEVCGIVSRIRDLVSTGT
                     PPGDILVLAQRSVIGTPIYQGLVNEGVPTISYYAESELESADAQKGFATLKLFVNRDD
                     RVALRWLLGLGSPNWHSPAYLRLRQYSEATGTTPWNVLERLSNGSISMPYTQPLIQRF
                     NELQNLLGELEQLTNLSAIIDRLFPSGNNEFVDFRELVSEVIAEVGEDDPVKFLSELT
                     SAIAKPEVPSEIEDVR"
     gene            1611..2015
                     /locus_tag="PKI68_06280"
     CDS             1611..2015
                     /locus_tag="PKI68_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53334.1"
                     /translation="MNFRIPRNIANYSPNPNPGTGMVLAGWWAGGKEFIGKGAGKVPK
                     KQRNKGTEFLISVAACGAGGNKTAPIRRRQGYGETSRTRLQLGFAVGWNAPFRRVFKL
                     SVRGAPAPLQLRASGRDAASALLEFTRQPPGL"
     gene            complement(2240..2974)
                     /locus_tag="PKI68_06285"
     CDS             complement(2240..2974)
                     /locus_tag="PKI68_06285"
                     /inference="COORDINATES: protein motif:HMM:NF019413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HNX53335.1"
                     /translation="MNLKLISCEIFYREMCAAVSTSPHRVDITFLPKGLHDLPPGQMP
                     AKIQEAIDAADGTCDAILLGYGLCNNGLVGIRAGNCPLILPRAHDCITLFLGGRDRYR
                     DYFDTHPGTYFLTSGWIERGETSGDLADLSVQAQLGMNLTMEELVEKYGEDNAEYLYE
                     TLCQGTRNYSHFAWIPMGVEPPDMEQTARQKAGEHGWSFETVPGDMTLIRKLVNGDWN
                     SADFLTVPPGSTVKAAYDGSIVASIQ"
     gene            complement(3024..>4108)
                     /locus_tag="PKI68_06290"
     CDS             complement(3024..>4108)
                     /locus_tag="PKI68_06290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HNX53336.1"
                     /translation="IIIGGGAAGFMAGVNAGERKIRTVILERCPKPGRKLLMCGNNRC
                     NITSALPVKEMLTDFGDPIAPFLEPALTAFPPSALREWCHKNGLVTKMQRDKRVYPAS
                     EKASDVHHLFTDLLAKYNVGIMLNSAVHKIIPLQTGEWLVKTDSAELTSRALLLATGG
                     VSYPKTGSVGDGQKMAAALGHKITPYRPGLAGFDMPPAWIAANPMRSIPNVTLKIIVD
                     GKTVATTEGFLEIEKYGIGGPAVTNASRIIARRDLKNYSFEVDMNGKKWTLNPLRVRP
                     LKEAMVTVGGIALNEVDSRTMESKKIPGLFFAGEILDIDGPSGGYNLQAAFSTARLAM
                     DAIARNLNRKSHFTEGNKGQQRGRPI"
     assembly_gap    4109..4208
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4209..5011)
                     /gene="trpD"
                     /locus_tag="PKI68_06295"
     CDS             complement(<4209..5011)
                     /gene="trpD"
                     /locus_tag="PKI68_06295"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNX53337.1"
                     /translation="MIKEAIQSLITGNPIDRDAAYRAIMTIMQGEATPAQIGAFITAV
                     RMHGATPEIIAGAAQAMRENMVKIRCADPNAVDIVGTGGDGAHTFNISTAAAFVTAGA
                     GVTVAKHGSYGVSSKCGAANVLSELGINLQYSPQKMEECLEKTGIAFLFAPALHPAMK
                     YAAGPRKELGFWSLFNILGPLCNPAGVKNGVLGVFSKELVPVVADACAQLGASYLFVV
                     RGNDGLDEITTTTTTLVTEIRRGKLETYEVQPAGLGLPQARTADITGGEP"
     gene            5087..6427
                     /gene="gcvPA"
                     /locus_tag="PKI68_06300"
     CDS             5087..6427
                     /gene="gcvPA"
                     /locus_tag="PKI68_06300"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNX53338.1"
                     /translation="MSISPFACTSPADEQAMLASIGAKSFDDLFAHIPAEFQSDEFHL
                     AEGLSEMEMMQQLRATARKNSSDLTNFCGAGFYDHFIPAAVSSLTSRGEFFTAYTPYQ
                     PEAAQGTLQAMFEYQTAITRLTNMEVSNASLYDGGTALFEGMMMALRISKRSRVLVDE
                     GVSPIYRTMLRSYTRNLKIEYREVAMSGGLADRDAFAKQLDSTVGAVLLQNPNFFGCL
                     DDLTGLIDQVHRAGAIAVVSAYPVSLGVVKTPGEMDADIVTGDGQSLGLPLSFGGPYL
                     GFMATKKKYVRNMPGRIVGATKDGQGRRGYVLTLQAREQHIRREKAASNICTNMQLCA
                     LRSLIYLSLLGKHGFVDVARQCMDRAGYAWTRLKAIKGVEPLYDRPFFNEFALKLPKD
                     AGEVVSELISEGIAAGFPAGRYYTGMENVLLCAFTEKRTKKEIDILAAKLEAVL"
     gene            6525..7970
                     /gene="gcvPB"
                     /locus_tag="PKI68_06305"
     CDS             6525..7970
                     /gene="gcvPB"
                     /locus_tag="PKI68_06305"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNX53339.1"
                     /translation="MKLIFEKSSPGRGGVRIPEDRISEHDIISENLLRAAPAELPEVS
                     ESEVVRHFTKLSRLNYSVDTHFYPLGSCTMKHNPRACEAAASLPDLCETHPLWPQLRH
                     GGLLTQGSLQILYSLERMLSEITGLAEFTLQPMAGSHGELTAVMMMAAYHRDRGNKKT
                     HIIIPDSAHGTNPASAALGGYEVVTIPSDASGDMDIELFKAALNDETAGVMLTLPSTL
                     GVFNPRVKEIIEAVHAVDGLMYYDGANFNAIMGRIKPGELGFDLCHLNLHKSFSTPHG
                     GGGPGTGPVGVCERLRPYLPISRVAKTKDGTYTLDYDFPKSIGYVAPFYGNFAILVRA
                     YAYLLMLGRDGLRATSDRAVLNANYVQEKLRPYFAAVTKGRCMHECVFSGKTLGGGVH
                     TLDLAKALLDRGYHAPTIYFPLNVPEAIMIEPTETETRETLDAFCADMIELAQLAKTN
                     PDALHQAPVTTPVGRLDEVAAAKNMELTYNG"
     gene            7963..8241
                     /locus_tag="PKI68_06310"
     CDS             7963..8241
                     /locus_tag="PKI68_06310"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HNX53340.1"
                     /translation="MVKHIVVWQMRESVSAEQKIEMKRRLETLVLRIPRVLKIEAGID
                     SGGGTMSLYSEFTSRDALAAYQTHPAHQEVVAFVKKLVTARSVCDYEC"
     gene            8231..9013
                     /locus_tag="PKI68_06315"
     CDS             8231..9013
                     /locus_tag="PKI68_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNX53341.1"
                     /translation="MSAEITSVQNERVKQIIKLQRKASARRAEGLTVIEGAREVSRAI
                     ENGWQPVELWLCSELGTGKPQIDTAGRECFQCSRAVFEKISYREGPDGVLAVGPLVGR
                     TLAELKLSKNPLILVAEGLEKPGNLGALLRTADGAGADAVIVCDSATDLNNPNVIRAS
                     IGTLFYLPVAEASSEEAIAFLKEQGVKVLSAVPDAEAVYTGVDLTGPVAIVVGAEDTG
                     LSSQWKGSADLNVKIPMLGKNDSLNVSVSAAILLYEAVRQRN"
     gene            9070..9146
                     /locus_tag="PKI68_06320"
     tRNA            9070..9146
                     /locus_tag="PKI68_06320"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9104..9106,aa:Met,seq:cat)
BASE COUNT         1849 a         2509 c         2682 g         2011 t
ORIGIN      
        1 cgaacatctt ctatctctga gggaacctcc ggcttggcga tcgcggatgt aagctccgaa
       61 aggaacttaa caggatcgtc ttcgccaact tccgctataa cttcgctcac gagttcacgg
      121 aaatctacaa attcattgtt cccagacggg aagaggcgat caatgattgc cgaaagattt
      181 gtgagttgct ctaactcacc caatagattc tgtaactcat taaaccgttg gatcaaaggt
      241 tgagtatatg gcatcgaaat cgatccattc gaaagcctct caagaacatt ccacggggtt
      301 gtgccggtcg cctctgaata ctgtctcaat cgtaaatagg caggagaatg ccagtttgga
      361 cttccaagtc ctagaagcca cctcaatgcg actctatcat ctcggttgac gaacaatttg
      421 agcgtagcaa accctttttg agcatcggct gattcaagct cagattctgc gtagtaagat
      481 attgtcggga ctccttcgtt gacgagacct tgataaatag gggttccaat cacgctgcgc
      541 tgagcaagaa ctaatatatc tcctggagga gttcctgtgg aaactaagtc tcgaattctt
      601 gaaacaatgc cacaaacctc ctcctgaagt gtggcgaact ggatcaactc gacaataccc
      661 tctccatttt cggctcgtgg tatcaactgc cgctgggtcg gccgatttgt gttatgctca
      721 atcagactat tcgccatctc ggtcactctt gttggacacc gacgacactc aataagagtt
      781 aagtcggcca aactaggccg cgaggcgatc cattctctga ttccctcggg atgtgcgtac
      841 ttaaagctat atatggactg atcgtcgtca ccaacaatgc agacctcagc attatcggac
      901 agtaaatcaa tgacaccttg ctctgctttg ttcaggtctt gatattcatc aacgagaata
      961 tgtccgtact cagttctttc cggtgctagg gggtttgatt gcaaatactg atatagcagc
     1021 ggaacaactt caccgatcag cattgagtta tgaaatatta accaacttaa tagatcgcga
     1081 ttaaatgcgt gatctgctgg attgattggg ctcccgggat cgtcatgctg taggcgtgcc
     1141 caagcagact gataagcctg aatttttttt cggacttcac gaagaccacc atgtgcagtc
     1201 aggtcgactt ctaatggagc catctcgaag ccattcaatg gtcttgggtt tcggcctgta
     1261 gcagataata catggttccg cataagaata cggagcgcga gactatgcaa agttgttccg
     1321 gttaattcac tacaccgagg gacattcatt ccgaccaact ctcggtggag atcctctgcc
     1381 gcgacgcggg taaatgttac cgctaaaata tcttttgggt ttgctccgtt ttctaggaga
     1441 cgagccaccc tccttttcat ggcaaaagat ttgccggttc ctgggccagc taagactcga
     1501 acattttgac ttgtcgagga agcaatatta taagcggggg taccttcttc aagactatca
     1561 gaccatgaca tagcttttgc ttcctttatt tcaaggacag gggttggcca atgaatttta
     1621 gaatcccaag aaacatcgcg aattattccc ccaatccaaa ccccggaacc ggaatggttt
     1681 tagcaggatg gtgggcgggc ggcaaagagt ttatcgggaa aggtgcgggg aaagtgccaa
     1741 agaagcaaag gaacaaaggc acagagtttt taatttctgt agctgcctgc ggtgcgggcg
     1801 gtaataaaac cgcgcccatc cgccgtcgcc aaggctatgg tgagacaagc cggacgcggc
     1861 tacagttggg atttgccgtt gggtggaacg cgccgttccg gcgcgttttt aaattaagcg
     1921 tgcgcggagc gcccgctcca cttcaacttc gggccagcgg cagggatgcc gcttctgcat
     1981 tgctggaatt tacaaggcaa ccgcccgggc tgtgacgccc gcgaagggtt gcgatacgta
     2041 ccgcaggcgt cccgcctgcc gccagagcac ttataaaaga aattcctccg cgatcttcgc
     2101 ggctctgcga ggaatcgttt atttactgga attgaacgcg ccgggacggc acgttccacc
     2161 tttgtagccg aggtccttga cctcggttcc gcgatcgtgg atcgcggcta caattctctg
     2221 cggctctgcg aggaatcgtt tattgaatgc tggcgacgat ggagccgtcg taggcggctt
     2281 tgaccgtgga gccgggcggg acggtgagga agtccgcgct gttccagtcg ccgttgacca
     2341 gtttgcggat gagtgtcata tcgccgggga cggtttcaaa actccagccg tgttcaccgg
     2401 ctttctgccg cgcggtctgt tccatgtccg gcggttcgac gcccattgga atccaggcga
     2461 agtggctgta gttgcgggtt ccctggcaga gcgtttcgta gagatattcg gcgttgtcct
     2521 cgccgtattt ttcgaccagc tcttccatcg tcaggttcat gccgagctgc gcctgtacgg
     2581 aaaggtcggc cagatcgccg gaggtttcgc cgcgttcgat ccagccgctg gtgaggaagt
     2641 aggtgccggg atgcgtgtcg aagtagtcgc ggtagcggtc gcgtccgccg aggaacagcg
     2701 tgatgcagtc gtgcgcccgc ggcagaatca gcggacagtt gccggcgcgg atgccgacca
     2761 gtccgttgtt gcagagtccg tagccgagca ggatggcgtc gcaggttccg tctgcggcgt
     2821 cgatggcttc ctggattttg gccggcatct ggcccggcgg caggtcgtgc agtcctttcg
     2881 gcagaaaggt gatgtcgacg cggtgcgggc tggtagagac cgccgcgcac atttcgcggt
     2941 aaaaaatttc gcagctgatc agtttcaggt tcataacagg gatattttta cagaaggtaa
     3001 cgaaggtaaa cgaaggagag attttagatg ggacgtcccc tttgttgtcc tttgtttcct
     3061 tctgtgaaat gactctttct gtttaaattt ctggcgatgg cgtccatggc gaggcgggcg
     3121 gtggagaagg cggcctgcaa attatagccg ccgctcgggc cgtcgatgtc gaggatttcg
     3181 cccgcgaaga aaagccccgg aatctttttc gattccatgg tgcgcgagtc gacttcgttg
     3241 agcgcgatgc cgccgacggt gaccatggct tctttgagcg ggcggacgcg cagcggattg
     3301 agcgtccatt tcttgccgtt catgtcgact tcgaagctgt agtttttcag gtcgcgccgc
     3361 gcgatgatgc ggctggcgtt ggtgaccgcc ggtccgccga tgccgtattt ttcgatttcc
     3421 agaaagcctt cggtggtggc gacggttttg ccgtcgacga tgattttcag cgtgacgttc
     3481 gggatgctgc gcatcgggtt ggcggcgatc cacgcgggcg gcatatcgaa gccggcgagg
     3541 ccggggcggt agggcgtgat tttatgcccg agcgcggcgg ccattttctg cccgtcgccg
     3601 acggagccgg ttttcggata gctgacgccg ccggtggcga gcaggagcgc gcgcgaggtc
     3661 agttcggcgc tgtcggtttt gacgagccat tcgccggtct gcagcggaat gattttgtgc
     3721 acggcgctgt tgagcatgat gccgacgttg tatttcgcca gcaggtcggt gaagaggtgg
     3781 tgaacgtcgg aggctttttc gctggccgga tagacccgct tgtcgcgctg cattttggtg
     3841 accagtccgt ttttatggca ccactcgcgc agggccgacg gaggaaacgc cgtcagcgcc
     3901 ggttcgagga acggggcaat cggatcgccg aagtcggtca gcatttcttt gacgggcagg
     3961 gcggaggtga tgttgcagcg attgttgccg cacatcagca gtttgcgccc cggcttcggg
     4021 cagcgttcga gaatgacggt gcggattttc cgttcgcccg cgttgacgcc ggccatgaag
     4081 cccgccgcgc cgccgccgat aatgatcann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnngg ctcgccgccg gtgatatcgg cggtgcgggc ctgcggcagt ccgagtccgg
     4261 cgggttggac ttcgtaggtt tcgagtttgc ctcggcggat ttcggtgacg agcgtggtcg
     4321 tcgtcgtcgt gatttcgtcg agtccgtcgt tgccgcggac gacaaacagg tagctggcgc
     4381 cgagctgggc gcaggcgtcg gcgacgaccg gcaccagttc ttttgaaaag acgccgagta
     4441 cgccgttttt aacgccggcc ggattgcaga gcgggccgag gatgttgaag aggctccaga
     4501 agccgagttc tttgcgcggc ccggcggcat atttcatcgc cgggtgcagc gccggggcga
     4561 acaggaaggc gatgccggtt ttttcgaggc actcttccat tttctgcggg ctgtactgaa
     4621 ggttgatgcc gagttcggaa aggacgttgg cggcgccgca tttgctggag acaccgtagc
     4681 tgccgtgctt ggcgacggtg acgcccgctc cggcggtgac gaaggcggcg gcggtcgaaa
     4741 tgttaaaggt gtgcgcgccg tcgccgccgg tgccgacgat atcgaccgcg ttcgggtcgg
     4801 cgcagcggat tttgaccata ttttcgcgca tggcctgcgc cgctccggcg atgatttccg
     4861 gcgttgcgcc gtgcatgcgg acggcggtga tgaacgcgcc gatctgcgcc ggcgtcgcct
     4921 cgccctgcat gatcgtcatg atggcgcggt acgcggcgtc ccggtcgatg gggttgcccg
     4981 ttatcagcga ctgtatggct tctttaatca tcggagtccc ttttgaactt gtccagtttc
     5041 ggggggagcg tatcatcggc ccccagtatt ttcaaagagg aaccctatgt ctatttcacc
     5101 gtttgcctgt accagtccgg ccgatgagca ggcgatgctt gcctccatcg gtgccaagag
     5161 ttttgacgac ctgttcgccc acattcccgc cgagttccag tcggacgagt ttcatctggc
     5221 cgaaggcctc tccgaaatgg aaatgatgca gcagctccgc gccacggcgc gcaaaaactc
     5281 ctccgacctg accaacttct gcggcgccgg tttctacgac cacttcattc ctgccgcggt
     5341 cagctcgctg acgtcgcgcg gcgagttttt caccgcctac acgccgtacc agccggaagc
     5401 cgcgcagggc acgctgcagg cgatgtttga atatcagacc gccatcacgc gcctgacgaa
     5461 catggaagtt tccaatgcct cgctttacga cggcggtacc gcgctcttcg aaggcatgat
     5521 gatggcgctg cgtatcagca agcgcagccg cgtgctggtg gacgaaggcg tcagcccgat
     5581 ctaccgcacc atgctgcgca gctacacccg caacctcaaa atcgaatacc gcgaagtcgc
     5641 catgtccggc ggcctcgccg accgcgacgc tttcgccaaa cagctcgaca gcaccgtcgg
     5701 cgccgtcctg ctgcagaacc ccaacttttt cggctgcctc gacgacctga ccggcctgat
     5761 cgaccaggtt caccgcgccg gagccattgc cgtggtttca gcttatccgg tttcgctcgg
     5821 cgtcgtcaaa acgcccggcg aaatggatgc cgatatcgtc accggcgacg ggcagagcct
     5881 cggcctgccg ctctcgttcg gcggcccgta cctcggcttt atggcgacga agaaaaaata
     5941 tgtccgcaac atgccgggcc gcatcgtcgg cgcgaccaaa gacgggcagg ggcggcgcgg
     6001 ctatgtgctg acgctgcagg cccgcgaaca gcatatccgt cgcgaaaagg cggcgtcgaa
     6061 catctgcacc aatatgcagc tctgcgcgct gcgttcgctc atttatcttt cgctgctcgg
     6121 caagcacggc tttgtcgatg tcgcccgcca gtgcatggac cgcgccggtt acgcctggac
     6181 gcgcctcaaa gccatcaaag gcgtcgagcc gctgtatgac cgtccgttct tcaacgaatt
     6241 cgcgctcaag ctgccgaaag acgccggcga agtcgtcagc gaactcatca gcgaaggcat
     6301 cgccgccggc ttccccgccg gtcgctatta caccggcatg gaaaacgttc tgctctgcgc
     6361 cttcaccgaa aagcgcacca agaaggaaat cgatattctg gccgcgaaat tagaggcagt
     6421 actttgaaat aaaaacgaac cactaattca cactaatgaa cactaatcag ggaacgcaat
     6481 tagtgagaat cagtgaaaat tagtggtttg aaaggttgtt ggttatgaaa ctgatttttg
     6541 aaaaaagttc gccgggccgc ggcggggtga gaattccgga agaccgcatt tcggagcatg
     6601 acattatcag cgaaaatctg ctgcgcgcgg cgcctgccga gctaccggag gtttccgaaa
     6661 gcgaagtggt gcggcatttt acgaaactgt cgcgactgaa ctattcggtc gacacgcact
     6721 tttatccgct cggctcctgc acgatgaagc ataatccgcg cgcctgcgag gccgcggcct
     6781 cgttgcccga tctgtgcgaa acgcatccgc tctggccgca gctgcgccac ggcggcctgc
     6841 tgacgcaggg ttcgttgcag attctttact cgctggagcg catgctttcg gaaatcaccg
     6901 gtctggccga gttcacgctt cagccgatgg ccggttcgca cggcgagctg acggcggtga
     6961 tgatgatggc ggcttatcac cgcgaccgcg gcaataagaa gacgcatatc atcattccgg
     7021 attccgcgca cggcaccaat ccggccagcg cggcgctcgg cggctacgaa gtggtcacga
     7081 tcccgtccga cgccagcggc gacatggata tcgagctgtt caaggcggcg ctgaacgacg
     7141 agaccgccgg cgtgatgctg acgctgccca gcacgctggg tgtcttcaac ccgcgcgtga
     7201 aggaaatcat cgaagcggtg cacgcggtgg acggcctgat gtattacgac ggcgcgaact
     7261 tcaacgccat catgggccgc attaaacccg gcgagctggg cttcgatctg tgccacctga
     7321 atctgcacaa gtcgttttcc acgccgcacg gcggcggcgg ccccggcacc ggaccggtcg
     7381 gcgtctgcga aagactgcgg ccctatctgc cgatctcgcg cgtcgccaaa accaaggacg
     7441 gcacctatac gctcgactac gactttccga agagcatcgg ctacgttgcg ccgttctacg
     7501 gcaacttcgc cattctcgtc cgtgcctacg cctatctgct gatgctcggc cgcgacggcc
     7561 tgcgcgccac cagcgaccgc gccgtgctca acgccaacta tgtgcaggaa aaactgcgcc
     7621 cgtactttgc agccgtcacc aagggccgct gcatgcacga atgcgtcttc agcggtaaaa
     7681 cgctgggcgg cggcgttcat acgctcgacc tggcgaaggc gctgctcgac cgcggctacc
     7741 acgcgccgac catttacttc ccgctcaatg tgccggaagc cattatgatc gagccgaccg
     7801 aaaccgaaac ccgcgagacg ctggatgctt tctgcgctga catgattgaa ttggcgcagc
     7861 tggccaagac caatcccgac gccttgcatc aggctccggt caccacgccg gtcgggcgtc
     7921 tcgatgaagt cgccgccgct aaaaatatgg agttaacata caatggttaa gcacatcgtt
     7981 gtctggcaga tgagggaaag tgtttctgcg gaacagaaga tagaaatgaa acggcggttg
     8041 gaaacgctcg ttctcagaat tccgcgggtt cttaaaatag aggcaggaat tgattccggc
     8101 ggcggaacca tgtcgctcta ttccgaattc acctcaagag acgctctggc cgcatatcag
     8161 acgcatccgg ctcatcagga ggttgttgct tttgtcaaaa agctcgtgac ggcgcggtct
     8221 gtctgtgact atgagtgctg aaatcaccag tgtgcagaat gagcgcgtga agcagatcat
     8281 caagcttcag cgaaaagcct ctgcccgccg cgcagaaggc ctgacggtga tcgaaggagc
     8341 ccgcgaagtg tcgcgggcaa tcgaaaacgg ctggcagccg gtcgaactct ggctttgctc
     8401 tgaacttgga accggtaaac cgcaaatcga cacggccgga cgcgaatgtt ttcaatgttc
     8461 gcgcgcggta tttgaaaaaa tttcctacag ggaaggtccg gatggcgtcc tggcggtcgg
     8521 cccgctggtc ggcagaacgc tcgctgaatt gaagctgtcg aaaaatcctt tgattctggt
     8581 tgctgagggt ctggagaagc cggggaatct cggcgcgctg ctgcgcacgg ccgacggcgc
     8641 gggcgcggat gccgtgatcg tttgtgattc ggcgactgac ctgaataatc cgaatgtcat
     8701 ccgcgcgagt atcggcacgc tgttttatct gccggttgcc gaggcgtcgt cggaagaggc
     8761 catcgcattc ctgaaagaac agggcgtaaa agttctctct gccgtgccgg atgctgaagc
     8821 tgtttatacc ggtgttgatc tgaccggacc ggttgcgatt gtggtcggcg cggaagatac
     8881 aggcctttcc agtcagtgga aaggctcggc ggatctgaac gtaaaaattc cgatgcttgg
     8941 aaaaaacgat tcactgaatg tttcggtttc tgcggcgatt ctgctttatg aggcggtccg
     9001 gcagagaaat taaatctttc cccttgcaac tgagctgaaa aactatacct tcaccacttc
     9061 gcaaataact gcggggtgga gcagtctggt agctcgtcag gctcataacc tgaaggtcct
     9121 gggttcaaat cccagccccg ctaccaactt t
//
LOCUS       DAOPZO010000080         9003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_9170_length_9003_cov_9.501565, whole genome shotgun sequence.
ACCESSION   DAOPZO010000080 DAOPZO010000000
VERSION     DAOPZO010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 9003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9003
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9170_length_9003_cov_9.501565"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PKI68_06325"
     CDS             <1..239
                     /locus_tag="PKI68_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53342.1"
                     /translation="HSVDLWTSGSVLYGMDGGRPITGLETVLTNGVLEVKFPVPGGKI
                     PSQVIIEEAGGGMDCGGGTLTELFKLPPLPASTP"
     gene            complement(270..1631)
                     /locus_tag="PKI68_06330"
     CDS             complement(270..1631)
                     /locus_tag="PKI68_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNX53343.1"
                     /translation="MKLKNGLLTTVGLLAATGMAQNTAAAKPNILWLLAEDISPGALS
                     CYGQEAASTPNLDQFARDGVRYTHFYTTAPVCSPSRSAFMTGMYATTIGAHQHRTKNK
                     KPLPDGIRPLPDWMRDAGYFTANLKTLPAPCGFRGTGKTDWNFSSDPNKAFDSSIWAD
                     LKTNQPFFAQINFEETHRDFHAARKVDPAKIKLPPYYPEHPVTRQDYAQYLDAAMELD
                     AKVGKVLAQLEKDGLAENTIVIFMGDNGEAHVRGKQFCYEEGLHVPMIIRWPAAVAAP
                     KQFKKGSVDDRLLEAIDMPPTFLSLVGAPIPPKMQGKAFLGDQAGAPKEYIFGARDNC
                     DTTVMRLRTVRDARFRYIRNFTPEVPFLAPSAYKEKQYPVWNLLKELNAAGKLTPDQA
                     VLCAPRQPEEELYDLQADPDEIHNLAKNPDYQAVLQRLRGVLEQWIIDSDDQGRFPAA
                     VRP"
     assembly_gap    1712..1811
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1812..3428
                     /gene="nadB"
                     /locus_tag="PKI68_06335"
     CDS             <1812..3428
                     /gene="nadB"
                     /locus_tag="PKI68_06335"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF006567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNX53344.1"
                     /translation="MQTRDTDFLVIGSGVAGLTAALRLSAHGQVLVASKREASECNTR
                     YAQGGIACVVDPADSMAEHISDTLQTGGGLSEESAVRAIVEGGPERIAELETLGVHFE
                     RNAEHPNDYDLGQEGGHSRRRVLHAGDITGQALSEGLLARAKADPNITIAENLLAIDV
                     IPTGWIGLNGENRCVGCYFLDTASGEVLAVRSPVTILATGGLGKVYLYTSNPDVATGD
                     GVAMAWRAGLPIRDMEMVQFHPTCLYHPDAKTFLISEAVRGEGARLIDRRGREFVYDF
                     DPRGPLAPRDITARAIDSVMKKYGDPFVYLDISHRDADFLQQRFPTLYKACLRYGFDM
                     ARAPVPVVPAAHYSCGGVIADVNGRTAMPGLYAVGEVASTGLHGANRLASNSLLEALV
                     CGHRCADEAVRHREENPVQNITIPRWECGDAVSSTEEIVVEHDWNEVRSVMWDYVGIV
                     RSDRRLARARRRIHTIREEIRSYYKEYLVTADLIELRNLAAVAELIVRSAQRRKESRG
                     LHYNEDWPETSAHARHTVIQDRPGGPLDVE"
     assembly_gap    3513..3612
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3613..4586)
                     /locus_tag="PKI68_06340"
     CDS             complement(<3613..4586)
                     /locus_tag="PKI68_06340"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNX53345.1"
                     /translation="MKKLHFRLRASGVIVASKSWAVLLTLSVAALAPAENNTNAAVRF
                     GIMSDIHQDIIPQAIRGVSNFIAAAQQEKADFIIQLGDFVQCRPENQPLITLWNSFPG
                     AKYCVLGNHEMDRKSTQAQMVEFLGMPAPYYTFTAGPIRGIVLNGNDAGGSAKGYPRF
                     IGETQLNWLKEQLAASRQPACIFIHQPFDGVNDTLGIQNAAAVRAVLEQAQAERPGSI
                     IAVFSGHLHEDYSFTTNGITYVEINSASYYWLDKTYLPKDAKGGPVKYDKPLWAFVTV
                     DLANGTVTLKGTAAEWTGKDPWKQGADETRIPHERVRPAISDRTFRFR"
     gene            4763..6046
                     /locus_tag="PKI68_06345"
     CDS             4763..6046
                     /locus_tag="PKI68_06345"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNX53346.1"
                     /translation="MKIKTGVMVFLLTAAAGFAQTEEEQFPPVPPLADVYQITTDLQI
                     AKVAKIVAQFRDPGDAVSALFTNTAEFGGGGPLRGPLVVTPMPPVPKKTYVPKNAPDT
                     LQMYIDKANPGDELVVAPGTYDTGGRRLNQEGPATRLVIDKPLTLRSEAGAELTIIAG
                     GPSTRCIYMTNGVRVIGFTISGGETMKEGPEGNKFTALSGGGVWSEPAGILEDCTVVS
                     NTALWYGGGLYGGNAIRCVFTGNTARRSGGGASKARLGNCLVQYNRAAHFGGGTHHCE
                     LRNCTVTHNRAEVMGAGAAFGMAQNTVLHHNQALLSGHNFLDVKMSYCCSMPLADGPG
                     NIGAEPGFKNTDQGVFLPTYDSALIDAGTNNFMDVDLAGNPRILDGNNNGHARIDIGA
                     YEYIHPKADSDHNGIPDRKEVPVKDASLRPEFPAE"
     gene            6175..>7651
                     /locus_tag="PKI68_06350"
     CDS             6175..>7651
                     /locus_tag="PKI68_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNX53347.1"
                     /translation="MATKQYVYFYGFGKEHTEGDKTMKAILGGKGANLAEMANAGLPV
                     PPGLTISTEACACYSKSGGKYPEGLDEQLKKTLGKLEKMMGKKLGDPNDPLLVSVRSG
                     AAISMPGMMDTVLNLGLTDKSVLGFIKQTGNERTAWDCYRRFIDMFGDVVMGPHTGLE
                     HHHFEVEIEKLKKKYKAKEDTDLTAEQLKELVDIYKKVYQNKVKKPFPQDPMEQLDLA
                     IRAVFGSWNSERAEKYRQINKVTGLLGTAVNICTMVFGNMGDESGTGVAFTRDGGTGD
                     SKPMGEYLINAQGEDVVAGIRTPKNIDDMPNEKSPVWGKAHSDLLKIMDRLEKHYKYP
                     QDVEFTVQEGKLWMLQTRNAKRTGLAGVRWAVEMVSGKDVYTGKTLSKILTPKEALMT
                     VSGEDLEQLLFPIFDIAAEKKARLITSALPAGPGAAVGKIVFDAKEAEHAVAKDPKAQ
                     VVLVRHETSPEDVGGMWAAQGVLTSTGGMTSHAAVVARGWGK"
     assembly_gap    7652..7751
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7752..>9003
                     /locus_tag="PKI68_06355"
     CDS             <7752..>9003
                     /locus_tag="PKI68_06355"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HNX53348.1"
                     /translation="STGGMTSHAAVVARGWGKCCICGAGALQIDYKKRTVTVGKKVYK
                     AGDFISLNGSTGMVYEGQIPTQSSPVVSGVVDNNTAARKHPIYKMYEQVSQWSDANRK
                     IHVRTNADTPKDAQNARAFGAEGIGLCRTEHMFFEGDRIWAIREFIIADDEAGRKKAL
                     AKLLKLQQKDFEGIFKAMDGLPVTVRLLDPPLHEFVPHTAKDQAEMAKRLNVPLKQVA
                     DRVQQLHEFNPMLGHRGCRLSVTYPELCVMQTTAIVAAACKVGKAKKKVKVLPEIMIP
                     LVGAKAELDFLVQIVRKTAEEIIRKEGVKLKYMVGTMIEIPRAALTADELAETAEFFS
                     FGTNDLTQMTFGFSRDDIGSFLPDYLKEKLLPCDPFQHLDQRGVGQLIQMGVQKGRST
                     RPDLKCGICGEHGGDPASVKFCCKA"
BASE COUNT         1821 a         2513 c         2658 g         1711 t
ORIGIN      
        1 gccattccgt ggatctgtgg accagcggat cggttctcta tggaatggac ggcggacggc
       61 cgattaccgg tcttgagacg gttctgacga acggcgtact cgaagtcaaa ttccccgttc
      121 ctggcggaaa gattccgtcg caggtaatca tcgaagaagc cggcggagga atggactgcg
      181 gcggcggaac gctgacggag cttttcaaac ttccgccgct tccggcttcg accccgtgat
      241 cctgaagttt tctgtagccg cgacggtggt cacggccgca ctgctgccgg gaaccggccc
      301 tggtcgtcgc tgtcgataat ccattgttcc agaacgccgc gcaggcgctg gagcacggcc
      361 tgatagtcag gattcttcgc gaggttgtga atctcgtccg ggtcggcttg cagatcatag
      421 agctcttctt cgggctgacg cggagcgcag agcacggcct ggtccggcgt gagctttccg
      481 gccgcgttca gctctttgag cagattccag acgggatact gtttttcctt atatgcgctc
      541 ggcgcaagga acggaacttc cggagtaaag ttgcggatgt agcggaagcg ggcgtcgcgc
      601 acggtacgga ggcgcatgac ggtggtgtcg cagttgtcac gcgcgccgaa tatgtattct
      661 ttcggcgcac cggcctggtc gccgagaaag gctttgccct gcatcttggg cgggatcggc
      721 gcgccgacga gcgagagaaa agtgggcggc atgtcgatcg cttccagcag gcgatcatcc
      781 accgaacctt ttttgaactg cttcggcgcg gcgaccgccg caggccagcg gattatcatc
      841 gggacgtgca acccctcctc atagcagaac tgcttgccgc ggacgtgcgc ttcgccgttg
      901 tcgcccatga agatcacgat ggtattctcg gccagaccgt ccttttcgag ctgcgcgaga
      961 acctttccga ccttggcgtc cagctccatg gcggcgtcga gatattgggc ataatcctgc
     1021 cgcgtgacgg gatgttccgg ataatacggc ggcagtttga tcttggcagg atcgactttg
     1081 cgggcggcgt ggaaatcgcg gtgtgtttct tcgaaattga tctgcgcaaa gaacggctgg
     1141 ttggtcttca gatccgccca gatgctggag tcaaaggcct tgttcgggtc agaagaaaaa
     1201 ttccagtctg tcttgccggt gccccggaag ccgcagggcg cgggcagggt tttcaggttg
     1261 gcggtgaaat atccggcgtc gcgcatccag tccggcagag ggcggattcc gtccggcaga
     1321 ggctttttat tcttcgtccg gtgctggtgc gcgccgatcg tcgtcgcgta catcccggtc
     1381 ataaacgccg agcgcgacgg cgagcagacc ggcgcggtgg tgtagaaatg cgtatagcgg
     1441 acgccgtcgc gggcgaactg atcgaggttc ggcgtcgagg ccgcctcctg gccgtaacag
     1501 ctgagcgctc ccgggctgat gtcctcggcg agcagccaga gaatattggg tttggcggcg
     1561 gccgtattct gcgccatgcc ggtggcggcc agcagaccga cagtggttaa aagaccgttt
     1621 ttcagcttca tagaccgcgc tcctttgatg ggtatcactc tgaagtttta gtcaaacgcc
     1681 cggcagtatt ccggtttacc cggcatttgt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn natgcaaaca cgcgacacag attttctggt gatcggcagc ggggtggcgg
     1861 gactgaccgc cgcgctgcgg ctgagcgcgc acggccaggt gctggtggct tccaagcgcg
     1921 aagcctccga atgcaacacg cgttatgcgc agggcggcat cgcctgcgtg gtcgatccgg
     1981 cggattccat ggcggagcac atttccgaca cgctgcaaac cggcggcggg ctcagcgagg
     2041 aatccgccgt acgcgccatt gttgaaggcg ggccggaacg gattgcggag ctggaaacgc
     2101 tgggcgttca tttcgagcgc aatgccgagc atccgaacga ctatgatctc ggccaggaag
     2161 gcggccactc tcgccgccgc gtgctgcatg ccggcgacat caccggccag gcgctgagcg
     2221 aaggtctgtt ggcccgagcc aaagccgacc cgaacatcac catcgcggaa aatctgctgg
     2281 cgatcgacgt gattccgacc ggctggatcg ggttgaacgg cgaaaaccgc tgcgtcggct
     2341 gctactttct cgataccgcg tccggcgaag tgctcgccgt gcgctcaccg gtcacgattc
     2401 tcgccaccgg cgggctggga aaagtttacc tttataccag caaccccgac gtcgccaccg
     2461 gcgacggcgt ggcgatggcg tggcgcgccg gtctgccgat ccgcgacatg gaaatggttc
     2521 agttccatcc gacctgcctt tatcatccgg acgcgaaaac ctttctgatt tccgaagcgg
     2581 tgcgcggcga aggcgcccgg ctgatcgacc ggcgcgggcg ggaattcgtt tacgacttcg
     2641 atccccgcgg accgctggct ccgcgcgaca tcaccgcgcg cgccatcgac tcggtgatga
     2701 agaaatacgg cgacccgttc gtctatctcg acatttcgca ccgcgacgcc gactttttgc
     2761 agcagcgctt tccgacgctt tataaagcct gcctgagata cggcttcgac atggcccgcg
     2821 cgccggtgcc ggtcgttccc gccgcgcact attcctgcgg cggcgtgatc gccgacgtca
     2881 acggccgcac ggcgatgccg ggactttacg ccgtcggcga agtcgccagc accggactgc
     2941 acggcgcgaa ccggctcgcc agcaattcgc tgctcgaagc gctggtctgc gggcatcgct
     3001 gcgccgacga agccgtccgg catcgggaag aaaatccggt gcagaacatc accattccgc
     3061 gctgggaatg cggcgacgcg gtttccagta cggaagaaat tgtcgttgaa cacgactgga
     3121 acgaagtgcg ctcggtcatg tgggactatg tcgggattgt ccgttccgac cgccggctgg
     3181 cgcgcgcgcg ccgccgcatc cacaccatcc gcgaggaaat ccgcagttac tataaggaat
     3241 atctcgtcac cgccgacctg attgaactgc gcaaccttgc cgccgtcgcg gaactgattg
     3301 tccgctccgc ccagcgccgc aaggaaagcc gcggactcca ttacaacgaa gactggccgg
     3361 aaacttccgc ccacgcccgc cataccgtca ttcaggatcg tcccggcggc ccgctggatg
     3421 tggaatagga tattctaagc cggatctgcg ggctgcgccc taagttccgg ctttactgta
     3481 ctgccgcagc tcagccgtat tccggcgtgg ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nntagcggaa gcggaaggtc cggtcggaaa tcgccgggcg cacccgttcg
     3661 tgcgggatcc gggtttcgtc ggcgccctgc ttccagggat ccttgcctgt ccattccgca
     3721 gcggttccct tcagggtcac cgtgccgttc gcgagatcga cggtgacaaa ggcccacagc
     3781 ggcttgtcgt atttcaccgg gccgcccttg gcgtcttttg gcagataggt tttatccagc
     3841 cagtagtagg aggcgctgtt gatttcgaca taggtgatgc cgttggtggt gaagctgtag
     3901 tcttcgtgca gatggcccga aaagacggcg atgatgctgc cggggcgctc ggcctgagcc
     3961 tgttccagca ccgcgcgcac ggccgccgcg ttctgaatgc ccagggtgtc gttgacgccg
     4021 tcaaagggct ggtgaatgaa aatacaggcg ggctgccggg aagcggccag ctgttctttg
     4081 agccagttca gctgcgtctc gccgatgaac cggggatatc ctttggcgct gccgcccgca
     4141 tcgttgccgt tcagaacaat gccgcggatc ggcccggcgg tgaaggtgta gtaaggagcc
     4201 ggcattccca gaaactcgac catctgcgcc tgcgtggatt tacggtccat ttcgtggttt
     4261 cccagcacac aatatttggc tcccggaaag ctgttccaca gagtgatcag cggctggttt
     4321 tccggccggc actggacgaa gtcgcccagc tgaatgatga agtctgcctt ctcctgctgt
     4381 gccgccgcga tgaagtttga tacgccgcga atcgcctgcg gaatgatatc ctgatggatg
     4441 tcgctcataa taccaaagcg cactgcggca ttggtgttgt tttcagcggg agccagtgca
     4501 gcgacggaca gggtcagaag gaccgcccat gatttgcttg caactataac gccggacgcc
     4561 cggagccgga aatgtaactt tttcatcgca cagcctttca ttgtttttaa gagcgcacag
     4621 gctagtcctt cgaaatggag ttgtcagttc atttacaggc aaagtaattt tcatgagtac
     4681 ggtttctccg gatgcggaat agagatttca atgggcagaa gaatccggta gaaaccatct
     4741 aatttctgga ggcgaggcgt ttatgaagat aaaaaccggt gtgatggtgt ttctgctgac
     4801 ggcagccgca ggatttgcgc aaaccgaaga agaacagttc ccgccggtgc ctccgctggc
     4861 ggatgtttat cagatcacga cggatttaca gattgcgaag gttgcgaaaa tcgtcgcgca
     4921 gttccgcgat ccgggcgatg ccgtttccgc gctgtttacc aatacggccg agttcggcgg
     4981 cggcgggccg ttgcgcggtc cgctggttgt gacgccgatg ccgccggttc cgaaaaaaac
     5041 ctatgttccg aaaaatgcgc ccgatacgct tcagatgtac atcgacaagg cgaatccggg
     5101 cgacgagctg gtggtggcgc cgggcacgta tgataccggc gggcggcggc tgaatcagga
     5161 gggcccggcg acgcggctgg tgattgataa gccgctgacg ctgcgcagtg aggcgggagc
     5221 ggagctgacg attattgccg gcggaccttc gacgcgctgt atttatatga cgaacggcgt
     5281 gcgggtgatc gggtttacga tcagcggcgg cgagacgatg aaggaagggc cggagggcaa
     5341 taagttcacg gcgctttccg gcggcggcgt ctggagcgag ccggcgggaa ttctggaaga
     5401 ctgcacggtg gtgagcaata cggcgctgtg gtacggcggc ggattgtacg gcggcaacgc
     5461 aatccggtgc gtttttaccg gaaacacggc gcggcgttcc ggcggcggcg cgagcaaggc
     5521 gcggctgggc aactgtctgg tgcagtataa ccgcgcggcg catttcggcg gcggtacgca
     5581 ccactgcgag ctgcgcaact gtacggtgac gcataaccgc gcggaggtga tgggcgccgg
     5641 tgctgcgttc ggcatggcgc agaatacggt gctgcaccac aatcaggcgt tgctgagcgg
     5701 acataatttt ctcgatgtaa aaatgagcta ctgctgcagt atgccgctgg cggacggacc
     5761 gggaaatatc ggtgcggaac cgggatttaa gaataccgat cagggcgttt ttctgccgac
     5821 ctacgattcc gcactgattg acgccgggac gaataatttc atggacgttg atctggccgg
     5881 caatccgcgt attctcgacg gaaacaacaa cggccatgcg cgcattgata tcggtgcgta
     5941 tgaatatatc cacccgaagg ccgacagcga tcacaacgga attccggacc gcaaggaagt
     6001 tccggtaaag gatgcgtcgc tccgtccgga attccctgct gaataagccg ttgcgactca
     6061 gccccgcctt gactctcctt ggtgctctgt tagcatcgtg catctttttt ttaagaaaga
     6121 aggcctttca tataaataga tgaagcaaaa ctgaaaacct aaaggagaaa cctgatggcg
     6181 actaaacagt acgtgtactt ctacggattc ggtaaagaac ataccgaagg cgataaaacg
     6241 atgaaggcca ttcttggagg caagggcgcc aatctcgcag aaatggcaaa tgccggcctt
     6301 ccggttcctc ccggtctcac gatttccact gaagcctgcg cctgctacag caagagcggc
     6361 ggcaaatatc ccgaaggttt ggacgagcag ctcaagaaaa cactgggtaa gctcgaaaag
     6421 atgatgggca aaaaactcgg cgatccgaat gatccgctgc tcgtctctgt ccgttccggc
     6481 gccgcgattt ccatgcccgg catgatggac accgttctca acctcggctt gaccgacaaa
     6541 tccgttctcg gcttcatcaa gcagaccggc aacgaacgca ccgcgtggga ctgctaccgc
     6601 cgcttcatcg acatgttcgg cgatgtggtt atgggcccgc ataccggcct cgaacaccac
     6661 cacttcgaag tcgaaatcga aaagctgaag aaaaaatata aagccaaaga agacaccgac
     6721 ctgaccgcgg aacagctcaa ggaactggtg gacatctata agaaggttta ccaaaacaag
     6781 gtcaaaaagc cgttcccgca ggatccgatg gaacagctcg accttgccat ccgcgccgta
     6841 ttcggttcgt ggaactccga acgcgctgaa aaataccgcc agatcaacaa ggtgaccggc
     6901 ctgctcggca ccgccgtcaa catctgcacg atggtgttcg gcaacatggg cgacgaatcc
     6961 ggtaccggcg tagcctttac ccgcgacggc ggaacaggcg actccaagcc gatgggcgaa
     7021 tacctcatca acgcacaggg cgaagacgtc gtggccggca tccgcacccc gaaaaatatt
     7081 gatgacatgc cgaacgaaaa atccccggtc tggggaaagg cgcactccga cctgctcaag
     7141 atcatggatc gtctcgagaa gcattataaa tatccgcagg acgttgaatt caccgttcag
     7201 gaaggcaagc tctggatgct ccagacccgt aacgccaagc gtaccggtct ggccggcgtc
     7261 cgctgggcgg tcgaaatggt ttccggaaaa gacgtttaca ccggcaaaac gctctcgaag
     7321 attctgacgc cgaaagaagc actgatgacg gtttcaggcg aagacctcga acagctgctg
     7381 ttcccgatct ttgatattgc ggccgaaaag aaagccaggc tgatcacctc cgcgctgccg
     7441 gccgggcccg gcgcggcagt cggcaagatc gttttcgacg ccaaagaagc cgaacatgcg
     7501 gtcgccaaag atccgaaagc tcaggttgtt ctcgtccgtc atgaaaccag ccccgaagat
     7561 gtcggcggta tgtgggcggc gcagggcgta ctcacttcca ccggcggcat gacctcccac
     7621 gcggcggttg ttgcccgcgg ctggggcaag tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn ntccaccggc ggcatgacct cccacgcggc ggttgttgcc cgcggctggg
     7801 gcaagtgctg catctgcggc gcaggcgcac ttcagattga ctataaaaaa cgcaccgtca
     7861 ccgtcggcaa aaaagtctac aaagcgggcg acttcatcag cctcaacggt tcaaccggta
     7921 tggtctatga aggccagatt ccgacacagt cgagcccggt tgtttccggc gtagtggaca
     7981 ataataccgc cgccaggaag cacccgatct acaaaatgta tgaacaggtt tcgcagtggt
     8041 cggatgccaa ccgtaaaatc catgtgcgca ccaacgccga tacgcccaaa gacgcccaga
     8101 acgcccgcgc gttcggtgcc gaagggatcg gtctctgccg tacggaacat atgttcttcg
     8161 aaggcgaccg catctgggca atccgcgagt tcatcattgc cgacgacgaa gccggccgca
     8221 agaaagccct cgccaagctg ctgaaacttc agcagaagga cttcgaaggc atctttaaag
     8281 cgatggacgg cctgccggtc acggtgcgtc tgctcgatcc gccgctgcat gaattcgtcc
     8341 cgcacaccgc gaaagatcag gctgaaatgg ccaagcgtct gaacgtcccg ctgaagcagg
     8401 tcgccgaccg cgttcagcag ctgcacgaat tcaacccgat gctcggtcat cgcggctgcc
     8461 gtctctcggt cacttatccg gaactctgcg tgatgcagac aaccgcgatc gttgccgccg
     8521 cctgcaaagt cggcaaagcc aagaagaaag tcaaagttct gccggaaatc atgattccgc
     8581 tcgtcggagc caaagccgaa ctcgacttcc tcgtacagat cgtccgcaag accgccgaag
     8641 agatcatcag gaaagaaggc gtgaagctca aatatatggt cggcacgatg attgaaattc
     8701 cgcgtgccgc gctgacggct gacgaactgg cggaaaccgc tgaatttttc agcttcggca
     8761 caaacgacct cacgcagatg acctttggat tcagccgcga cgacatcggc tccttcctgc
     8821 cggattatct gaaagagaag ctgctgccgt gcgacccgtt ccagcacctc gaccagcgcg
     8881 gcgtgggcca gctgattcag atgggcgttc agaaaggccg ttcgacccgc ccggatctca
     8941 agtgcggtat ctgcggtgaa cacggcggtg acccggccag cgtgaagttc tgctgtaaag
     9001 cag
//
LOCUS       DAOPZO010000081         8990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_9186_length_8990_cov_9.627644, whole genome shotgun sequence.
ACCESSION   DAOPZO010000081 DAOPZO010000000
VERSION     DAOPZO010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8990
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9186_length_8990_cov_9.627644"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PKI68_06360"
     CDS             <1..316
                     /locus_tag="PKI68_06360"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNX53349.1"
                     /translation="HPRAYGSFTKVLRAALDGRAAPLPETIRKMTSLPAEHFRLNGRG
                     VLKKGNFADVAVFDPQTIEEKSTYANPHQLSAGIAALVVNGTLTIKDGNLTGERSGKF
                     LD"
     gene            379..879
                     /locus_tag="PKI68_06365"
     CDS             379..879
                     /locus_tag="PKI68_06365"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HNX53350.1"
                     /translation="MNGKIQSFPFSADNNARVLILGSMPGAESLRQQQYYAHPRNLFW
                     DIMGDLFGAGRELPYEERLAVLRENGVALWDVAHSCRRNGSLDSNMTDVAANDFQRLF
                     ATAPQIHTVFFNGQTAAALFRRLVRPDLNHELQLVVLPSTSPANASVPAGKKKAAWSK
                     VQTALK"
     gene            complement(922..1731)
                     /locus_tag="PKI68_06370"
     CDS             complement(922..1731)
                     /locus_tag="PKI68_06370"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNX53351.1"
                     /translation="MTEFWTIYWMAVVFIAGLCLGSFLNVCICRIPQEKSVAWPPSAC
                     PNCNNRIKWYDNIPVLSWFLLKAKCRHCSLPISIAYPIIELLTGFFFLTIWLIYGFTW
                     FTPIYALAVFGLLLATFIDLQEMWLPDRVTIGGMILFPILSTLFPPLQGEITWAGGLK
                     DSLIGLAAGFGLLWIVRELGTAALKQEAMGFGDVKLMGALGALFGWEAVIYIIFFSAF
                     IGSIAGISLIALHRKGIQSQIPYGPYISLAAFSWLLGGFRLWDAYLRLMGL"
     gene            complement(1728..2573)
                     /gene="aroE"
                     /locus_tag="PKI68_06375"
     CDS             complement(1728..2573)
                     /gene="aroE"
                     /locus_tag="PKI68_06375"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNX53352.1"
                     /translation="MKKLAVLGHPISHTLSPVMHNASIKALGLDGQYEYGKLDVPPGG
                     LMARLAQLPAEGYAGVNLTIPLKEVAFKGLKNLADSARILGAVNTVEFTAGGLVGHNT
                     DGYGFLKALEEAFGKTLEGDAVFVLGCGGAGRAAALMAALDGAQSITLADVDAGRVEN
                     LSAEIEQLAPQVGIFQCLEKITQIELCREADLVIQASPVGMKKTDPSLLPEEAFREGQ
                     RAFDLIYMYPETAFLSTAKAAGAQIANGLGMLLHQGAKAFNIWTGIEPDVAAMRKALE
                     KAVYP"
     gene            complement(2570..4255)
                     /locus_tag="PKI68_06380"
     CDS             complement(2570..4255)
                     /locus_tag="PKI68_06380"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNX53353.1"
                     /translation="MKTPKYKAPPPFEMPDRQWPSRTLAVSPSWCSVDLRDGNQALPN
                     PMDPDQKLEYFQMLCRIGFKEIEAGFPSASQDDFDFFRCLIEENHIPDDVFIMGLTQC
                     RSHLIDRTFEAFKGVKKGIVHAYIATSELHMKQVFGTDQKGTMKAAVEATRRIRALAD
                     TMPESDIRYEFSPEEFTDTDMPFCLELCKAVYDAWGRATPEKPLILNLPATVERRPPN
                     HYADMIEWFIRHFPHRDSVKISLHAHNDQGMAVAATELALMAGGDRVEGTLFGHGERT
                     GNVDLVTVANNLFSRGIETGLNFSSLSEIAATVERLTGMPVYYRQPYAGEYAFTAFSG
                     SHQDAINKGMHRLSEAGKIFGMKWKVPYLHVDPADLGRNFEKLIRINSQSGKGGIAWV
                     LEQDYGIQVPKAMQPELREEVQLYSEAVGREISSEEVHTVFREKFVNPAGPFKLIGYW
                     PRPDDKDPTFIHGEVRIDVSGVEKTAVADGNGPISAFVHAVRSIVGIDFSVADYHEQA
                     VGKGADAQAMAYVPLKLEGNGIVFGVGSDSNIDQAAVRAVIAGLNRIAGKKQK"
     gene            complement(4478..5269)
                     /locus_tag="PKI68_06385"
     CDS             complement(4478..5269)
                     /locus_tag="PKI68_06385"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase-associated EpsI family protein"
                     /protein_id="HNX53354.1"
                     /translation="MEIRPVTPHLILIGLMIITALALAFTVDVALSDQAGIRLELPAT
                     LDGGWAGDQLRYSHDPENPQQYRVSELELPNIDPKTGKKLFTMSLAEYDALPKDTEFI
                     KSIYTNATAKDVFVSIVLSGRERDSIHRPERCLVGQGSTIINQHFWNVIMPQIIEVPL
                     EGRAPLRVAVLETVRNYRDANGERKTYNGYYAYWFVGQNRETPSHYARMLWLAWDRVV
                     NSVANKWAYIAIAGDRGDAKSDAYKQDIIDFVQKLYPHLLISKQG"
     gene            complement(5239..6216)
                     /locus_tag="PKI68_06390"
     CDS             complement(5239..6216)
                     /locus_tag="PKI68_06390"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HNX53355.1"
                     /translation="MQNQSSIKVWSERWRLGALSKPELVQIGLVSVIVGLIFMMFHML
                     GNTVVNVGSRSAFQWMFSRWQDTISFGADYSHGYFIPFISLGVIWYKRKEIFSVEKYT
                     DSRGLGVVIFALVLHWLGAKMQQTHFSLASLVLLIWGIPFYLFGWELAKKLLFPCAYL
                     VFCIPLNFMDVMAFPLRIFSTRLATDMLNGIGIGAIRSGTAIYSNVAGGFEFDVADPC
                     SGLRSLLAMTALTAVYAYFTQRTTLRKWILFMASIPLAVVGNIARIVTVAIVAEAFGG
                     KLALGIYHDYSGYILFTAAITMMVVIGGVLNLEFSEVMKKWKSVLLHRT"
     gene            complement(6262..8019)
                     /locus_tag="PKI68_06395"
     CDS             complement(6262..8019)
                     /locus_tag="PKI68_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53356.1"
                     /translation="MRKHLNILLLAAVAIFSLWCTGGLMRKVDQASVLAGAYDMAQGR
                     MQDPSAYYQYDKTYVLYRVCAGILKLLPEGVDPVAATNIVLALFFWAALTLFTVRFRR
                     TLHPLALLCFLSAPAVLLNTFYVNSSVLSSAFLLLSAIFVFQAGKRGGWMAAIFFALS
                     AGSRADAVLLLPLLIWLITPRSEVEGLISAIREKKSLLRKQADFSKQWKLLTAGISAL
                     AAGWILCPAGGASSDPVFNFKMIAGYAVFGFGAAGLLFIVYCGYLAAPVVRKGSGWQE
                     RIYQAAGLGALVLPVLFFIPQLHTPRYFWLGCNAVLFMSAAKPSLPEPGRAARVLLMA
                     AALLPLVIGVRLPALSRPQIVAAHSGGFYPTGDGFYPMGGTLPFMFRLKDAAARPVDH
                     NQLIWNAMRQARFEFNENRSINVLSTPMFGYFMLEASLQRGKAERCSPEDVQNGRFYA
                     DSRSLMRDDPKSPINKLSEILLLPARFISPVRDGVGILEFGAGTDEWGRQTRMLNRLF
                     GGNEYRIADAAGSGTPDRKTVVFSKQPFAGAVQDDVSGLYYSEEPASVPVLWSFRAVA
                     VFPEWMSIRVFKGRDYKNL"
     gene            complement(8016..>8990)
                     /locus_tag="PKI68_06400"
     CDS             complement(8016..>8990)
                     /locus_tag="PKI68_06400"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNX53357.1"
                     /translation="MKRSLVTGGCGFVGRHLIARLLKAGHDVYCVDPIAPLSGGIAPE
                     QWPLFSPLDFKNFHFYRQDCRDWFEQNSDAGFDYAFHLAAMVGGREMIENNPLAVADD
                     LSIDARYWQWAVKARPAKNVVFSSSAAYPVELQRPGQYVLLKEEMITFKTRIGMPDMT
                     YGWAKLTNEYLAKLAFEKHGLESVCYRPFSGYGEDQHDSYPFPGICKRVLANRNASEI
                     TVWGSGRQMRDFIHIEDCVTGILGTMDRINDASALNLSTGLYTSFIEFAQTAAEICGF
                     RPDVRGTSDKPEGVFARGGDTALQRKMGFVPAVSFRDGIAKAVEYFSA"
BASE COUNT         2149 a         2543 c         2455 g         1843 t
ORIGIN      
        1 gcatccgcgc gcctacggca gtttcaccaa agtgctgcgc gccgcgctgg acggccgggc
       61 cgcgccgctg ccggaaacga tccgtaagat gacgtcgctg cccgccgaac acttccggct
      121 gaacggccgc ggcgtgctga agaaaggcaa cttcgccgac gtggctgttt tcgatccgca
      181 gaccattgag gaaaagagca cctacgccaa cccgcatcag ctttccgcag gcatcgcggc
      241 gctggtcgtc aacggaacgc tgacgatcaa ggacgggaat ctgaccggcg agcgaagcgg
      301 aaaattttta gactgatcat tacaaggcag gcaggatgcc tgcggtacgt atcgcaggca
      361 tcctgcctgc ctttagttat gaatggaaaa atccagagtt ttccgttttc agcagacaat
      421 aacgcgcggg ttctgattct cggttcgatg ccgggagcag aatcactgcg acagcagcag
      481 tattacgcgc atccgcgcaa tctgttctgg gacatcatgg gcgatctgtt cggcgcgggc
      541 cgcgagcttc cgtacgaaga gcgtctggcg gttctgaggg aaaacggcgt cgctctctgg
      601 gacgttgcgc acagctgccg ccgtaacgga agtctcgatt ccaatatgac cgatgtggcg
      661 gcgaatgatt ttcaaaggct gttcgcgaca gcgccgcaga ttcatacggt cttttttaac
      721 gggcagactg ccgccgcgct gttccgcaga ctggttcggc ccgatctaaa ccacgagctg
      781 caactggttg tcctgccttc aaccagtccg gcaaatgcgt cggttcccgc cggaaagaaa
      841 aaagcggcct ggagcaaagt ccagaccgct ttaaaataac ccaccccggc cttcgtccac
      901 ccttcccgtg gaggggattt ttcacaaacc catcaagcgg aggtaggcgt cccagagacg
      961 aaaaccgccg agcagccagg agaaggcggc gagagagatg tacggaccat acggaatctg
     1021 gctctgaatt cctttgcggt gcagcgcgat gaggctgatg ccggcgatgg aaccaatgaa
     1081 cgctgaaaag aaaatgatgt agatgacggc ttcccagccg aagagcgcgc cgagtgcgcc
     1141 catcagtttg acgtcgccaa aacccatcgc ttcctgcttg agcgcggcgg tgccgagttc
     1201 gcgcacaatc cagagcaggc cgaagccggc ggccagaccg atcagtgaat ctttaagccc
     1261 gcccgcccag gtgatctcgc cctgcaacgg cggaaaaagc gtgctgagaa tcggaaaaag
     1321 aatcataccg ccgatcgtaa cgcggtcagg gagccacatt tcctgaaggt cgataaaggt
     1381 tgcaagcagc agaccgaata cggcgagcgc gtagatgggg gtgaaccagg taaagccgta
     1441 gatgagccag atggtgagga agaaaaatcc cgtgagcagt tcaatgatcg gataagcgat
     1501 cgaaatgggc agactgcaat ggcggcattt ggctttgagt aagaaccagc tcagcaccgg
     1561 aatattgtcg taccatttga tgcggttgtt gcagttcgga caggctgacg gcggccaggc
     1621 aacagatttc tcctgcggga tccggcagat gcagacattg agaaaacttc ccagacagag
     1681 gccggcgata aaaaccaccg ccatccagta gatcgtccag aattctgtca tgggtatacc
     1741 gccttttcta atgctttacg cattgctgca acatcgggtt caattccggt ccagatatta
     1801 aacgcctttg cgccctgatg caacagcatg cccaaaccat tggctatctg cgcgccggca
     1861 gctttggcgg tgctgagaaa agcggtttca ggatacatgt aaattaagtc gaaggcgcgc
     1921 tgtccttcac ggaacgcttc ttccggcaaa agcgacgggt cggttttttt catgccgacc
     1981 ggcgaagcct gaatcaccag atccgcttcg cggcagagtt caatctgcgt gattttttcc
     2041 agacactgga aaatgccgac ctgcggagcc agctgttcta tttccgcgga aagattttca
     2101 acacgaccgg catcgacatc cgcaagggtg atcgactgcg ctccgtcgag tgccgccatc
     2161 agcgcagcag cacgtcctgc cccgccgcag ccgaggacaa agacagcatc gccttcaagg
     2221 gttttgccga aggcttcttc aagtgctttg agaaaaccgt agccgtcggt gttgtgaccg
     2281 accagtccgc ccgctgtaaa ttcaactgta ttgacggcgc caagaatgcg ggcgctgtct
     2341 gccagatttt tgagtccctt gaacgcgacc tctttcagcg gaatcgtcag attcactccg
     2401 gcgtagcctt cggcgggcag ctgcgcaagg cgggccatca gcccgccggg cggaacgtcg
     2461 agcttgccgt attcatattg gccatcgagc ccgagcgcct tgatggaggc gttgtgcata
     2521 acgggcgaca gcgtgtggct gatcggatgt ccaaggactg ccagtttttt catttctgtt
     2581 ttttccctgc gatacggttc agtccggcaa tgacggcgcg aacggcggcc tggtcgatgt
     2641 tggagtcgga gccgacaccg aagacgatgc cgttgccttc cagtttcagc ggaacgtagg
     2701 ccatcgcctg tgcatccgcg cctttgccga cggcctgttc gtgatagtcg gcgacgctga
     2761 agtcgatgcc gacgatgctg cgcaccgcgt gcacgaaggc ggagatcggg ccgttgccgt
     2821 cagcgacagc ggttttttcg acaccgctga cgtctatgcg gacctcgccg tgaatgaagg
     2881 tcgggtcttt gtcgtcagga cgcggccagt aaccgatcag tttgaacggg ccggcaggat
     2941 tgacaaattt ttcgcggaag acggtatgaa cttcttccga tgaaatttcg cggccgaccg
     3001 cttcactgta gagctgcact tcctcgcgca gttcaggctg catggcctta ggcacctgga
     3061 tgccgtagtc ctgctccagc acccaggcga tgccgccttt gccggactgc gagttaatgc
     3121 ggatcagttt ttcgaagttt cggcccagat cggccggatc aacgtgcagg tacggcactt
     3181 tccatttcat gccgaatatt tttcccgcct cgctcaggcg atgcatccct ttgttgatgg
     3241 cgtcctgatg cgaaccggag aaggccgtga aggcatactc gccggcgtac ggctggcggt
     3301 aataaaccgg catgccggtc agccgctcga cggtggcggc aatttcgctc aggctggaaa
     3361 agttcagtcc ggtttcaatg ccgcgcgaaa acagattgtt ggcaaccgtg accaggtcga
     3421 cattgccggt acgctcaccg tgtccgaaca gggtgccttc aacgcggtcg ccgccggcca
     3481 tcagcgccag ctcggtggcc gcaacggcca tgccctgatc gttgtgtgca tgcagcgata
     3541 tcttaacgct gtcgcggtgc gggaagtgtc tgatgaacca ttcgatcatg tcggcataat
     3601 ggttgggcgg ccgccgttcg acggtggccg gcaggttcag aatcaacggc ttttcaggag
     3661 tcgctctgcc ccaggcgtcg taaacggctt tgcacagttc cagacagaac ggcatgtcgg
     3721 tatcggtaaa ttcttcgggt gagaattcgt agcggatgtc gctttccggc atggtgtcgg
     3781 ccagcgcacg gatgcggcgg gtggcttcca ccgccgcctt catggtgcct ttctgatccg
     3841 tgccgaaaac ctgtttcatg tgcagctccg acgttgcgat gtaggcatgc acaattcctt
     3901 ttttgacgcc tttgaaggcc tcaaaggtgc ggtcgatcag atgcgaacgg cactgcgtca
     3961 gccccatgat gaacacatcg tccggaatat ggttttcttc gatcaggcag cggaaaaagt
     4021 cgaagtcatc ctgactggcg gacgggaatc ccgcttcgat ttctttgaag ccgatacggc
     4081 acagcatctg aaagtattcc agcttctgat cggggtccat cggattgggc agcgcctgat
     4141 tgccgtcgcg caggtcaaca gagcaccagg acgggctgac agccagtgtc cgtgagggcc
     4201 attgacgatc cggcatttca aacggcgggg gggccttgta tttcggtgtt ttcattgctc
     4261 tttcctttac tgttttttct ctcttaaacc cactatgaaa ataaaaaaac ctctccgggc
     4321 tgcggagagg ttcggcaaaa tcattgttgg agggtcagat caacaagcgc agaactcccg
     4381 cagggtttcc ccggcgagca gaaggagttc aaggctgttg aatttatgtc tcattgcgga
     4441 aaggatggta aatgcacact gcggattttg tcaaccctca tccctgcttg ctgatcagca
     4501 gatgcggata gagcttctga acgaagtcga taatgtcctg tttataggca tccgacttcg
     4561 catcaccccg gtcgccggca atcgcaatat aggcccattt attggcgacg ctgttcacaa
     4621 cacggtccca ggccagccag agcatacggg catagtggct gggagtttca cggttctgac
     4681 cgacaaacca gtaggcgtaa tagccgttat aggttttacg ttcgccattg gcatcgcggt
     4741 aattgcgaac cgtttccaga acagccaccc tgagcggcgc ccgaccttcc aacggcacct
     4801 caatgatctg cggcataatc acattccaga agtgctggtt gatgatcgtg ctgccctgcc
     4861 cgaccagaca gcgttccgga cggtgaatac tgtcacgctc gcggccggaa agcacgatgg
     4921 agacaaaaac gtctttggcg gtcgcgttgg tataaataga tttaatgaac tcggtgtctt
     4981 tcggcagtgc atcgtattca gccagactca tggtaaagag ctttttaccc gttttcggat
     5041 caatgttcgg caattccagt tcactgacgc ggtactgctg cggattttcg ggatcgtggg
     5101 aatagcgcaa ctggtcaccc gcccaccctc catcaagtgt ggcaggcagc tccaggcgaa
     5161 tcccggcctg atcgctcagc gcaacgtcga cagtaaacgc caaagccagt gctgtaataa
     5221 tcatcaagcc gatgagtatc aggtgcggtg taacaggacg gatttccatt ttttcattac
     5281 ctcactgaat tcaagattca acacgccgcc gataacgacc atcatggtga tggcggctgt
     5341 aaaaaggata tagccggagt agtcatgata aatgccaagc gccagctttc cgccgaaggc
     5401 ttcggccaca attgccacgg taacaatacg tgcgatgttg ccgacaacgg ccagaggaat
     5461 agaggccatg aaaagaatcc atttacgaag ggttgtgcgc tgagtgaagt aggcgtaaac
     5521 ggccgtcagc gccgtcatcg cgagcagtga ccgcagaccg ctgcaagggt cggccacatc
     5581 gaactcaaag ccgccggcaa catttgaata aatggccgtt ccggaacgaa tggctccgat
     5641 tccgatgccg ttcagcatat ccgtagccag tctggttgag aaaatacgca ggggaaaagc
     5701 catgacgtcc atgaagttca acgggataca gaacaccaga taggcacagg gaaaaagcag
     5761 ctttttcgcc agctcccagc cgaacagata aaacggaatt ccccagatca gcagaacaag
     5821 actcgccagc gaaaagtggg tctgctgcat tttggcgcca agccagtgca aaaccagagc
     5881 gaaaatgacc acacccaatc cgcgtgaatc ggtgtacttt tcgacagaaa agatttcttt
     5941 ccgcttatac cagattacac ccaggctgat gaagggaatg aaatacccgt gagaatagtc
     6001 cgctccgaaa gaaatcgtgt cctgccagcg ggaaaacatc cattgaaacg cagagcggct
     6061 gccgacgttc acaacggtgt ttccaagcat gtgaaacatc atgaatatca ggccgacgat
     6121 tacagaaacc agcccgatct gcaccaactc cggcttactc agagccccga gccgccagcg
     6181 ttcgctccag actttaatag atgactgatt ttgcataaca tctctcaatg ggtaaaaaat
     6241 tatatcatac ggcgaccaga atcacagatt tttatagtct ctgcccttaa acacccggat
     6301 tgacatccat tcaggaaaaa ccgcaaccgc ccggaaactc cacagcaccg ggacagaagc
     6361 cggttcttca gaataataca atcccgacac atcatcctga acggcccctg caaacggctg
     6421 tttcgaaaaa acgaccgtct tacggtccgg cgtcccggat ccggcagcat cagcaatccg
     6481 gtattcattc ccgccaaaaa gacggttaag catccgggtc tgccgtcccc actcgtcggt
     6541 accggcaccg aattcaagaa ttcccacacc gtcccggacc ggagaaataa accgtgcggg
     6601 cagcagcaga atttcactca gtttattgat agggcttttc gggtcatcac gcatcagact
     6661 gcggctgtct gcataaaaac gcccgttctg cacatcctcc gggctgcacc gttcagcctt
     6721 cccgcgctgc aacgaagcct ccagcatgaa atatccgaac atcggagtgc tcagcacgtt
     6781 aatcgagcgg ttttcattga actcaaaccg agcctgccgc atcgcattcc agatcagctg
     6841 attgtgatct accggccgcg ccgccgcatc cttcaaccgg aacataaacg gaagcgttcc
     6901 ccccatcggg taaaatccgt cgccggtggg ataaaatcct ccggaatggg ccgcgacaat
     6961 ctgcgggcgg ctcagcgccg gaagacgcac acctataacc agaggcagca gcgccgccgc
     7021 catgagcagt acgcgcgccg cccggccggg ctccggcaaa gaaggctttg ccgccgacat
     7081 aaaaaggacg gcattacacc cgagccagaa ataacgcgga gtatgcagct ggggaataaa
     7141 aaacagtacg ggcagaacca gcgcgcccag cccggcggcc tgatatatcc gctcctgcca
     7201 tccggagcct tttctgacaa ccggcgccgc gaggtatccg caatatacga taaaaagcaa
     7261 tccggccgcg ccgaatccga aaaccgcgta ccctgcaatc atcttgaaat taaacaccgg
     7321 atcagaagat gcccctccgg cggggcacag gatccatccg gccgccaacg cagaaattcc
     7381 ggccgtcagc agtttccact gcttggaaaa gtccgcctgt tttctcaata gagacttttt
     7441 ttctctgatt gcgctgatca gcccttcgac ttcagaccgc ggcgtgatca gccaaatcag
     7501 caacggcagc aaaagcactg cgtcggcacg cgaaccggcg gacagagcaa aaaagatcgc
     7561 cgccatccag cccccgcgct ttccggcctg aaagacaaag attgcactca gaagaagaaa
     7621 cgcagacgac agaactgagc tgttcacata aaaggtgttc aacaggacag ccggagcaga
     7681 aagaaaacac agcagcgcca acggatgaag tgtcctgcga aaccgcacag taaagagtgt
     7741 cagcgcggcc cagaagaaca gcgccaaaac gatgtttgtt gcggcgaccg gatcaacgcc
     7801 ttccggaagc agctttaaaa tcccggcgca aacccggtac agaacgtagg ttttatcgta
     7861 ctgatagtac gcagacggat cctgcatacg gccttgagcc atatcatatg ccccggccag
     7921 aacggatgcc tgatcgacct ttcgcatcaa ccctccggta caccaaagcg aaaagatcgc
     7981 gacggccgcc agcagcagaa tgttcagatg ttttctcatg cgctgaaata ttcgacggct
     8041 tttgcaatac cgtctctgaa agacaccgcc ggaacaaacc ccatcttccg ttgcagagcc
     8101 gtatctccgc cgcgggcgaa aacgccttcc ggcttatccg aagttccgcg aacatccggc
     8161 ctgaatccgc agatttccgc ggcggtctgc gcaaattcaa tgaaggatgt gtagaggccg
     8221 gttgaaagat taagcgcaga ggcgtcgtta atccggtcca tcgttccaag aattccggtc
     8281 acacaatctt caatatggat aaagtcgcgc atttgccgtc cggaacccca gacggtaatc
     8341 tccgaagcgt tacggtttgc cagcacccgc ttgcaaatcc cgggaaacgg ataagagtcg
     8401 tgctgatctt cgccgtatcc ggaaaacggg cggtagcaga cggattcgag tccatgcttt
     8461 tcgaaggcaa gtttagcgag atattcattg gtcagcttgg cccagccgta ggtcatgtcc
     8521 ggcatgccga tgcgggtttt gaaggtgatc atttcttctt tcagcaagac atactggccg
     8581 ggccgctgaa gttcgaccgg ataagccgcg ctggagctaa acacaacatt ctttgcgggg
     8641 cgggctttaa ccgcccactg ccagtaccgg gcgtcaatgg aaagatcgtc cgcgacggcc
     8701 agcggattgt tctcgatcat ctcgcgcccg ccgaccattg cggccagatg aaaggcatag
     8761 tcaaacccgg catcggaatt ctgctcaaac cagtcgcggc agtcctgccg gtaaaaatga
     8821 aaatttttga aatccagagg gctgaacagc ggccactgct ccggtgcaat cccgccgctg
     8881 agaggagcaa tcggatcgac gcaataaacg tcgtgcccgg ctttcagcag ccgcgcaatc
     8941 aggtgccgtc cgacaaatcc gcatccgcct gttaccagtg atcttttcat
//
LOCUS       DAOPZO010000082         8918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_9312_length_8918_cov_10.574298, whole genome shotgun sequence.
ACCESSION   DAOPZO010000082 DAOPZO010000000
VERSION     DAOPZO010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 8918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8918
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9312_length_8918_cov_10.574298"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1610)
                     /locus_tag="PKI68_06405"
     CDS             complement(<1..1610)
                     /locus_tag="PKI68_06405"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX53358.1"
                     /translation="MTALLNEAEKLIRAGRYEEAVAMLCRIGPPEGARICELIARAYR
                     GRNRARGDVYTAKVFAEQAIAHGSTDPEMPKIIAHAAVPNLPLKHPLVPQKFKMGGPK
                     EKRQEADAPYSFCALSKLCGKADAPKDFQWLEKNIPCQKACPASTDIPGYLDAIYRGE
                     HSKAYRINLEANVFPAVLGRVCARPCEAACRHGWDGLGEPVAICFSKRSAADFGGQAP
                     VVLDKWFGPSGKKIAVIGAGPAGLAAARQLARLGHQVTVYEKHNKPGGMMNQGIPVFR
                     LPRDLINKEIGQIEALGVQIICNAEIGRTLPLKQLAEQFDAVILAAGTLKPNLLDLPG
                     KELRGIRHGLDFLLEVNETGKGDVGKTVVVIGGGFTAMDCARTAKRLGAQVKVCYRRT
                     AAEMLVTPGELEELEHEGIPMEYMVAPKAYTGVDGRLKTIQFVRTEMGAPDPSGRRRP
                     VEIPGSEFELPVDSVLLATGQFPDTSWLDETSSHLNVFSAGDFKNGASSLIQAIGHAK
                     ECVREVDRFLTGRDRLREVALIEDAEETG"
     gene            complement(1607..3193)
                     /locus_tag="PKI68_06410"
     CDS             complement(1607..3193)
                     /locus_tag="PKI68_06410"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNX53359.1"
                     /translation="MAAQPELTQVERDKLEINPDFDFREIAKRPFEEITPNELSMFKW
                     SGVYHQLQPSFFMIRVVTPGGLMTTKQFRRAVDLAEEFAQGELCITTRQTLQFHWIRK
                     EDIYKIIEGMADVGITTKNGCGDVPRNIIGSSLAGASQEEIGDSYKIIQMLATDHEIR
                     DLQRNLPRKHKISVAFANEAGAQTLMNCQGWVPVKRGDTVGWKFHAGGGLGARPYLAK
                     VIFDWVPEELVLPVAQATVEAFRRHGDRRQRAFARLKIVVDKMGSSGFADVLLDIMKE
                     RGVRGLEKIEKAASPIPNIGRMVLNGQEVIPQKQAGLSVVRVMIPRSELKTAQSRTIA
                     RLADELGGGKIMFTNRQNMEFHDVPERNVPALLDALHTAGFRTSGHEHLPDIVACVGT
                     TMCKMAVADCPDVYHRLYDALAGDEKYWRAVGPLRINITGCPNNCAHAWIADIGLRGK
                     RTRNESSGSTEGYTVFVGGKLSEAGKIGEQLCDVDAAEIVPAVKKILDAYLKHRLSQS
                     ELFTDFCERIGIAAFRNLVK"
     gene            3337..4407
                     /locus_tag="PKI68_06415"
     CDS             3337..4407
                     /locus_tag="PKI68_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNX53360.1"
                     /translation="MSNTFNPVPLVLFGVPFHNVTFDEAIQWAVDRVRSGKPGVIATA
                     NLDFLVLAQNDPQLREVLYEADLVIADGFPPVKLAPFYGPPLKGRVTGSDITPMLAER
                     AAREGISVYGLGAAEGVAAKAMEVLKKRHPELNVAGAWSPPYAPLDQMNHAEILRRLD
                     EAKPDILFTAFGAPKQDKFNHLHVKTWKVPVAIGIGGTLDFIAGVQTRAPVWVQKIHC
                     EWLWRWGTDPKRLTKRYVTNIGFLIRAVWLTLKLRLGKNIPATVDPSAPPHWPAHGVT
                     YHEFQDLETVPGDLSGNVVMDLRNVEWLSSLEIGVLLELSKRVRKVVLLHAGSKVRKL
                     FEFSKLTDRLPLAETCAEALRL"
     gene            complement(4480..4968)
                     /gene="tadA"
                     /locus_tag="PKI68_06420"
     CDS             complement(4480..4968)
                     /gene="tadA"
                     /locus_tag="PKI68_06420"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNX53361.1"
                     /translation="MDETFSEDVVYMRMALREAERAAEAGEVPCGAIIVKDGAIIGKA
                     HNQTELLKDPTAHAEVLAITQAAAALENWRLTDAVMYVTKEPCPMCAGAIVLARLKKV
                     VWAVDDPKRGGARSKFEILDHADLNHRVEIQTGVLEAECKSLLQGFFRERRAEKKQDL
                     DL"
     gene            complement(4986..5591)
                     /locus_tag="PKI68_06425"
     CDS             complement(4986..5591)
                     /locus_tag="PKI68_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNX53362.1"
                     /translation="MSDNWSLFLSVWIKFSFVFTPFFALSMFLSMTENYSESRRRKLA
                     LAVSGSVAVICFILFFAGNQLFSLFGITLNSFRIGAGALLFLSAVALVQGSTATADAN
                     REGDIAVVPLAIPIIVGPATTGTLLVLGAELNGATAKSLGSLALLLAVTGLGVLLLSG
                     SLIQRSLGNRGIAILSKLTGLILAALAAQMIMTGIQGFLAH"
     gene            complement(5655..>8918)
                     /gene="smc"
                     /locus_tag="PKI68_06430"
     CDS             complement(5655..>8918)
                     /gene="smc"
                     /locus_tag="PKI68_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNX53363.1"
                     /translation="DLGLNEVSITRRVFRSGESDYFINKKPCRRKDIQRLFMDTGIGT
                     DAYSVMEQGKIDRVLSSRPDDRRAVFEEASGITKFKTDKQEALRKLEQTEANLLRLAD
                     VIREVKRQIISLQRQAGKAQRFKTLQEELRALDIYSTRHRLKQLSTRIEEIRAQLEIF
                     RVKVDETHHRISAANGETDALRAGLQQLETAIGTAMDDSMRVKNELERAQQTIRTNAE
                     RITELSTLAQQNTKESEEAKSRLTQHRASLDEVVQMMQSAEAERLKAKAELETATARQ
                     RETETQVQTSREALNRLRNESMTLDSRIGRLQNELSEIDAKERAGVIRKEQLAAEKIS
                     LERALASYEERREQMAARAEEYKLSVADQQEAMAELRDRRSSAAEKIQALEKEISELQ
                     RKLADKSARLEMVFEARKEGFPAGAQHLLDRRDENVVGPLAEQFIAAPGFETALEAVL
                     RPWMDALVVRTRSGARNVLTLLAESNQGAVRLLAAEGEAPSAPAENSLLGRISCADEV
                     RPLAARLLANVFVAENLQSIPLTLPAGSVFVTKRGELLTAEGAAELWKPEEGEVNPLG
                     RHHLREQLQSDIQSLEKALTDKRRMLEALRAEQSGTGDALEAAQRELDERRRSLAVQE
                     GESQVIARELKQARDRVETVTFEFNKLMQQQGSGEDRRTAVAREGAEARDRLAQIRQQ
                     ITTQTETLNALEEQRANVLSETSECRVQYSQHEQRIESLKSRRQPLEARLKELQELIE
                     ERTRGVSSYQQRVAQLKTASSEAEEKVEPLLEAVAAAEQKLSEARQIREVHTATLSER
                     EKSLRALQNELEELQSGKTKLEVELAEQRMRHQNSIDRLTSTWHITPDQLAREPEPKW
                     ENGVEPPMEEIETMAAEHRAKLEAIGPVNLVAIEEHAELEERFAFLSQQEADLISAKD
                     QLVEMIKKINLTTTELFKETFDKVNENFGVIFEQLFGGGSAKLVLVDETDVLESGIEI
                     IARPPGKKLQTISLLSGGERTMTAVALLFALFKVKPSPFCVLDEMDAALDEANVSRFV
                     KMVKGFLSQSQFVLITHSRQTISAADVIYGVTMPNRGVSRVMSMKFSDYQDSDAAKK"
BASE COUNT         1587 a         2582 c         2758 g         1991 t
ORIGIN      
        1 ccggtctctt cggcgtcctc aatcagcgca acctcgcgca gccggtcgcg gccggtcaga
       61 aaacggtcca cctcgcggac gcattccttg gcgtggccga tggcctgaat cagcgagctg
      121 gcgccgtttt taaaatctcc ggcggagaac acgttgaggt ggctggaggt ttcatcgagc
      181 cacgacgtat cgggaaactg cccggtcgcc agcagaacgc tatccaccgg cagctcgaac
      241 tcgctgcccg gaatttcgac cggacggcgg cggccgcttg gatcgggcgc gcccatttcg
      301 gtgcggacaa actggatggt tttcaggcgg ccgtcgacgc cggtataggc cttcggcgcc
      361 accatgtact ccatcggaat cccctcgtgt tccagctctt cgagctcgcc gggggtcacc
      421 agcatctccg ccgcggtccg gcggtagcag accttcacct gcgcgccgag ccgcttggcg
      481 gtgcgcgcgc agtccatcgc cgtaaagccg ccgccgatca ccaccacggt tttcccgacg
      541 tcgccttttc cggtttcatt gacttccagc agaaagtcga ggccgtggcg gatgccgcgc
      601 agctctttgc ccggcagatc cagcaggttc ggcttcagcg taccggcggc cagaatcacc
      661 gcgtcgaact gctccgccag ctgtttcagc gggagcgtcc ggccgatttc ggcgttgcag
      721 ataatctgca cccccagcgc ctcgatctgg ccgatttcct tgttgatcaa atcgcgcggc
      781 aggcggaaca ccggaatgcc ctgattcatc atgccgccgg gcttattgtg cttttcataa
      841 accgtcacct ggtggcccag ccgcgcgagc tgccgcgccg ccgcaagccc cgccgggccg
      901 gcgccgatca cggcgatttt tttgccgctc ggcccaaacc atttatccag aacgaccggc
      961 gcctgtccgc cgaagtcggc cgccgagcgc ttggaaaagc agatcgcgac cggctcgccg
     1021 aggccgtccc agccgtggcg gcaggcggcc tcgcaggggc gcgcgcaaac ccgcccgagc
     1081 acggccggga agacattggc ttcgaggttg atccggtagg ccttgctgtg ttcgccgcga
     1141 taaatcgcat cgagataacc gggaatatcg gtcgaggccg ggcaggcctt ctggcacgga
     1201 atattttttt ccagccactg gaaatctttc ggcgcgtccg ctttgccgca caatttggag
     1261 agcgcgcaga aagaataagg cgcatcggct tcctgacgtt tttctttcgg tccgcccatt
     1321 ttgaatttct gcggaacaag aggatgtttg agcggaagat tcggcacggc ggcgtgcgca
     1381 ataatcttcg gcatttccgg atcggtgctg ccgtgagcga tggcctgttc agcaaaaact
     1441 ttcgccgtgt aaacatcacc gcgcgcacgg ttgcggccgc gataagctct ggcaatcagt
     1501 tcgcagatgc gcgcgccttc cggcggtccg atgcggcaga gcatggcgac cgcttcttcg
     1561 taacgaccgg cccggatcag tttttcggct tcgttcaaca gtgcagtcat ttgacaaggt
     1621 tccggaaggc cgcaattccg atccgttcgc agaaatcggt gaaaagctcg ctttgactga
     1681 gccggtgttt cagataggcg tccagaatct tttttaccgc cggaacaatt tcagccgcat
     1741 caacgtcgca gagctgttcg ccgattttac cggcttcgct gagtttgccg ccgacaaaaa
     1801 cggtatagcc ttcggtgctt ccgctgcttt cattgcgcgt gcgtttgccg cgcagtccga
     1861 tgtcggcgat ccaggcatgg gcgcagttat tcggacagcc ggtaatattg atccgcagcg
     1921 gcccgacggc tcgccagtat ttttcatcac cggccagtgc gtcgtaaagg cggtgataaa
     1981 cgtccggaca atctgccacc gccatcttgc acatggtcgt gccgacgcag gcgacaatgt
     2041 ccggcagatg ctcgtgtccg ctggtgcgga atccggccgt gtgcagagcg tccagcagcg
     2101 ccggcacatt ccgctccggc acatcatgaa attccatatt ctgccggttg gtgaacataa
     2161 tcttcccgcc gcccagttcg tcggccagcc gcgcaatcgt ccttgattga gcggttttga
     2221 gttccgagcg cggaatcatt acgcgtacga cgctcaagcc cgcctgtttt tgaggaatca
     2281 cttcctggcc gttcagaacc attctgccga tgtttggaat cggcgaggcc gccttttcta
     2341 ttttttcaag accccggaca cctcgttcct tcatgatatc aagcaggacg tcggcgaacc
     2401 cggatgagcc cattttatcg accacgattt tcaggcgggc gaaagcgcgc tggcggcggt
     2461 cgccgtgacg gcggaacgct tcgacggttg cctgcgccac cggcagaacc agctcttccg
     2521 gcacccagtc aaaaatcacc ttcgccagat acggccgcgc gccgagcccg ccgcccgcgt
     2581 gaaatttcca gccgacggtg tcgccgcgtt tgaccggcac ccagccctga cagttcatca
     2641 gcgtctgcgc gcctgcttca ttggcgaatg cgacgctgat cttgtgtttg cgcgggaggt
     2701 tgcgctgcaa atcccggatt tcgtggtcgg tcgccagcat ctggataatt ttgtacgagt
     2761 cgccgatttc ttcctgcgat gcgccggcca gcgacgaccc gataatattg cgcggtacgt
     2821 cgccgcagcc gttttttgtg gtgattccca catcggccat gccctcgatg attttgtaga
     2881 tgtcttcttt gcggatccag tggaactgca gcgtctggcg ggtggtgatg cacagctcgc
     2941 cctgtgcgaa ctcttcggcg agatcaaccg cgcggcggaa ctgcttcgtt gtcatcagtc
     3001 cgccgggcgt aaccacgcgg atcatgaaaa agctgggctg gagctgatga tagacgccgc
     3061 tccacttgaa catggacagc tcgttcggag tgatctcctc gaaggggcgt ttggcaatct
     3121 cgcggaaatc gaaatccggg ttgatttcga gcttgtcgcg ctctacctgc gttaattccg
     3181 gttgtgcggc catgtggact ccttgttgaa tcagaatttg atagaaaacc atacgcagaa
     3241 accggccgga aagcaataca cataaaacgt gataacatta atcattaagc agagtgacgc
     3301 ttgaaagcac cgtcgcgttg acttaacctc cgcggcatgt ccaacacctt caatccggtt
     3361 ccgctggtac tcttcggtgt accgtttcat aacgtcacct ttgacgaagc catacagtgg
     3421 gccgtcgatc gcgtccgctc cggaaaaccc ggcgtcatcg ccaccgccaa cctcgacttt
     3481 ctggtgctgg cgcaaaacga tccgcaattg cgcgaagtgc tttatgaagc cgacctggtg
     3541 attgctgacg gctttccgcc ggtcaaactg gcgccgtttt acggcccgcc gctgaaagga
     3601 cgggtaaccg gcagcgacat cacgccgatg ctcgccgaac gcgccgcgcg cgaaggcatc
     3661 agcgtctacg gcctcggtgc cgctgaaggc gtcgccgcga aagcaatgga ggtcctcaag
     3721 aaacggcatc cggaattgaa cgtggcgggc gcgtggtcgc cgccgtacgc gccgcttgat
     3781 caaatgaatc acgccgagat tctgcgccgc ctcgatgagg cgaaaccgga catcctcttt
     3841 accgccttcg gcgcgccgaa gcaggacaag ttcaaccacc tgcacgtcaa aacatggaag
     3901 gttcccgttg ccatcggcat cggcggcacg ctcgacttta tcgccggagt ccagacccgc
     3961 gcaccggtct gggtacaaaa gattcattgc gaatggctct ggcgctgggg taccgatccg
     4021 aagcggctga cgaaacgcta cgtcaccaac atcggatttt tgattcgtgc agtctggctg
     4081 acgctgaaac tgcggctggg aaaaaatatt ccagcgaccg ttgatccgtc ggctccgccg
     4141 cactggccgg cacacggcgt gacctatcat gaatttcagg atttggaaac ggtgccgggc
     4201 gatttgtccg gcaatgtggt gatggacctg cgcaatgttg aatggctcag cagtctggaa
     4261 atcggcgtgc tgctggaact cagcaagcgg gtccggaaag tggttctgct tcacgccggc
     4321 tcaaaagtcc ggaagctgtt cgaattctct aaactcaccg accggcttcc gctcgccgaa
     4381 acctgcgccg aagcgcttcg attgtaggac gcgcttctgc gaagcagagc gtgtgaatgt
     4441 tttaaacaca ggctctccgg aataccggag aagcctgtcc tacaaatcta aatcctgttt
     4501 cttctcagcg cggcgttcgc ggaagaagcc ttgaaggaga gatttacatt cagcttcgag
     4561 aacgccggtc tggatttcaa cgcggtggtt gaggtcggcg tggtcgagga tttcaaattt
     4621 agagcgcgcg ccgccgcgtt tcggatcgtc gacggcccag acgacttttt tgagccgcgc
     4681 cagtacgatg gctccggcgc acatcgggca gggctctttg gtgacgtaca tgacggcatc
     4741 ggtcagccgc cagttttcga gcgcggcggc ggcctgtgta atcgcgagca cttcggcgtg
     4801 cgcggtcgga tctttgagca gctcggtctg gttgtgcgct ttgccgatga tcgcaccgtc
     4861 tttgacgata attgcgccgc agggcacttc gccggcttcg gcggcgcgct ctgcttcgcg
     4921 cagcgccatc cgcatataca ccacatcttc tgaaaaggtt tcgtccatag ttttaccaat
     4981 cgatgtcagt gcgccagaaa tccctgaatg ccggtcataa tcatctgggc ggccagcgcg
     5041 gcgagaatca ggccggtcag cttgctgaga atggcgatac ctctgttgcc cagcgaacgc
     5101 tgaatgagcg agccggaaag aagcaggacg ccaagtccgg ttacggcgag cagcagcgcg
     5161 aggctcccaa ggctttttgc tgtggcaccg ttcagttcag cgcccagaac gagcagagta
     5221 cccgtagttg ccgggccgac aatgatggga atggccagcg ggaccacggc aatatcgcct
     5281 tcgcggtttg cgtccgctgt tgccgtgctg ccctgcacca gagcgaccgc cgacaggaag
     5341 aggagcgccc ctgcgccgat ccggaaggag ttgagtgtga taccgaacag agagaagagc
     5401 tgattgccgg cgaagaacag aataaaacaa atgacggcca ccgatccgga aacggccagc
     5461 gccagtttgc ggcggcggct ttcgctgtag ttctccgtca tgctgagaaa catggacagc
     5521 gcgaagaacg gcgtgaatac aaaagaaaac ttaatccaca cgcttaaaaa cagggaccag
     5581 ttatcgctca tcggtatgac tccttaatca ttgatccgcc gccgcagtga acggttaaaa
     5641 cccggcgcta cattctactt tttggcggcg tcggagtcct gatagtcgga gaacttcatg
     5701 gacatgacgc gcgagacgcc gcggttgggc atggtgacgc cgtagatgac gtcggccgcc
     5761 gaaatggtct ggcggctgtg cgtgatcaaa acaaactgcg actggctgag gaagcctttg
     5821 accattttca cgaaccggct gacgttggct tcgtcgagcg ccgcgtccat ttcgtcgagt
     5881 acgcagaacg ggctcggttt gactttgaag agcgcaaaca gcagcgccac ggcagtcatc
     5941 gtacgttcgc ccccggagag cagcgaaatg gtctgaagtt ttttgccggg agggcgggcg
     6001 ataatttcaa tgccggattc cagcacgtcg gtttcgtcga ccagcaccag tttggcggag
     6061 ccgccgccga agagctgctc aaagattacg ccgaagtttt cgttcacttt gtcaaaggtc
     6121 tctttaaaca gctcggtggt ggtcaggttg atcttcttga tcatttcgac gagctggtct
     6181 ttggcgctga tgagatcggc ttcctgctgg ctgaggaagg cgaagcgttc ttcgagttcg
     6241 gcgtgttctt cgatggcgac aagattgacc gggccgatgg cttcgagttt ggcgcggtgc
     6301 tcggccgcca tcgtttcgat ttcttccatc ggcggctcta cgccgttttc ccatttcggt
     6361 tcgggttcgc gggcgagctg atcgggcgtg atgtgccacg tcgaagtcag ccggtcgatt
     6421 gagttctggt ggcgcatccg ctgttcggcc agttccactt ccagtttagt cttaccgctc
     6481 tgcagttctt ccagctcgtt ctgaagcgcg cgcaggctct tttcgcgttc ggaaagggtg
     6541 gcggtatgga cttcgcggat ctgccgggct tcggacagtt tctgttctgc ggcagcgaca
     6601 gcttcgagca gcggctcgac tttttcttcg gcttcgctgc tggcggtttt gagctgggcg
     6661 acgcgctgct ggtaggagct gacgccgcgg gtgcgttctt cgatcagttc ctgcagttct
     6721 ttcaggcgcg cttccagcgg ctggcggcgg ctcttcagcg actcaatccg ctgttcgtgc
     6781 tgggaatact gcacgcggca ctcggatgtt tcggaaagca cgttggcgcg ctgttcttcc
     6841 agggcgttca gcgtttcggt ctgcgtggtg atctgctggc ggatctgcgc cagccggtcg
     6901 cgggcctccg cgccttcgcg ggcgacggcg gtacggcggt cttcgccgga accctgctgc
     6961 tgcatcagtt tattaaattc aaaggtgacg gtttcgacgc ggtcgcgcgc ctgtttgagt
     7021 tcgcgggcga tgacctggct ttcgccttcc tgtacggcga gggagcggcg gcgttcgtcg
     7081 agttcgcgct gggcggcttc gagcgcgtcg cccgttccgc tctgttcggc gcgcagggct
     7141 tcgagcatcc ggcgtttgtc cgtcagcgct ttttccagcg actgaatatc gctttgcagc
     7201 tgttcgcgca gatggtggcg gccgagcgga ttgacttcgc cctcttcggg tttccagagt
     7261 tcggcggcgc cttcagcggt cagcagttcg ccgcgtttgg tgacaaagac ggaaccggcg
     7321 ggcagggtga gcggaatcga ctgaagattt tcggcgacga agacattggc gagcagccgc
     7381 gcggccagcg ggcggacttc atcagcgcag gaaattctgc cgagcagaga gttttcggcg
     7441 ggtgcggacg gcgcttcgcc ttcggcggcg agcaggcgta cggcgccctg attgctttca
     7501 gccagcagcg tgaggacgtt gcgcgcgccg gagcgggtac ggacgaccag cgcgtccatc
     7561 cacggacgga gaacggcttc gagcgcggtt tcaaaaccgg gagccgcgat aaactgttcg
     7621 gccagcgggc cgacgacgtt ttcgtcgcgg cggtcaagca ggtgctgcgc gccggcgggg
     7681 aagccttctt tacgggcttc aaaaaccatt tcaaggcgcg cgctcttatc cgccagcttg
     7741 cgctggagtt cggaaatttc tttttccagc gcctggattt tttcggcggc ggaggagcgg
     7801 cggtcgcgca gttcagccat tgcttcctgc tggtcggcga cggacagttt atattcttcg
     7861 gcccgtgccg ccatttgctc ccggcgttct tcgtaggagg ccagcgcccg ttcgaggctg
     7921 attttctcgg cggcgagctg ttctttgcgg ataacgccgg cgcgttcctt ggcgtcgatt
     7981 tcggaaagtt cgttctggag gcggccgatc cggctgtcga gcgtcatgga ttcgttgcgc
     8041 aggcggttca gcgcttcgcg gctggtctgc acctgagttt cggtttcgcg ctggcgggcg
     8101 gtcgcggttt cgagttccgc tttggctttc agccgttccg cttcggcgga ctgcatcatc
     8161 tgtacgactt cgtcgagcga agcgcggtgc tgcgtcaggc ggcttttcgc ctcttcagat
     8221 tctttggtgt tctgctgggc gagtgttgag agttccgtga tgcgctcggc attggtgcgg
     8281 atggtctgct gtgcgcgttc cagctcgttc ttaacgcgca tcgagtcgtc catggctgtg
     8341 ccgatagcgg tttcgagctg ctggagtccg gcgcgcaagg cgtcggtttc tccgttggcg
     8401 gcggagatgc ggtggtgggt ttcgtcgact ttgacgcgga agatttcgag ctgtgcgcgg
     8461 atttcttcaa tgcgggtgct cagctgtttg aggcggtggc gggtggaata gatatcgagc
     8521 gcgcgcagtt cttcctgcag cgttttaaag cgctgggctt taccggcctg ccgctggagg
     8581 gaaataatct gccgttttac ttcgcggata acgtcggcga ggcgcagcag gttggcttcg
     8641 gtctgttcga gcttgcgcag agcttcctgt ttgtcggttt tgaacttggt gatgccggaa
     8701 gcttcttcga aaacggcgcg gcggtcgtcg gggcgggaag aaagtacgcg gtcgattttc
     8761 ccctgctcca tgacggagta ggcgtcggtg ccgataccgg tatccatgaa aagacgctgg
     8821 atgtctttgc ggcggcaggg ctttttgttg atgaaatagt cgctttcgcc ggagcggaaa
     8881 acgcggcggg tgatactgac ttcgttcagg ccgagatc
//
LOCUS       DAOPZO010000083         8761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_9524_length_8761_cov_8.807259, whole genome shotgun sequence.
ACCESSION   DAOPZO010000083 DAOPZO010000000
VERSION     DAOPZO010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 8761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8761
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9524_length_8761_cov_8.807259"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /gene="rfaP"
                     /locus_tag="PKI68_06435"
     CDS             complement(<1..741)
                     /gene="rfaP"
                     /locus_tag="PKI68_06435"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011703.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HNX53364.1"
                     /translation="MMDLNTHFKKAWAAADPFAAAFRLEGEVFRSVKTRRTFRFELGG
                     KSYFAKVHHGIGWAEILKNLLQLKRPVFSARNEYEAIRRLEKLGVATMKIAAFGERGR
                     NPARIESFIITDELVNTVSLEDYCRDWKRNPPPFELKLALIRYVAQVSRLLHCNGVNH
                     RDYYLCHFLLDVSRDWKAGIKASLIDLHRAQLRKKTPRRWAVKDVAGLYFSAMNIGLT
                     SRDRLRFMEIYSGGSLRETLRNGAGFWRA"
     gene            complement(738..1856)
                     /locus_tag="PKI68_06440"
     CDS             complement(738..1856)
                     /locus_tag="PKI68_06440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX53365.1"
                     /translation="MTIAFCLFKYFPYGGLQRDFLRIALECQRRGYSIAVYTLSWDGP
                     VPPGFDVCLVPVRALNNHRRAKKYFQWVEKNAAAGSVRVGFNKMPGLDVYYAADACYK
                     EKSLTQRGPFYRLGARYRLYSAFEKAVFGKDSATELLMISKTQEPFFVKHYGTQPERM
                     HFLPPNIARDRIAPPDAPKLRAAFRRAHGLSDETRLLVQIGSGFIKKGVDRSIRALAA
                     LPPDVRERTVLWVVGKDRAARFEKLARDLGVGGRVIFTGGRDDVPQILLGADLMIHPA
                     YDENTGTVLLEAVAAGLPVLCTEVCGYGSHIRTAQCGLLTPEPFDQAVLNHQLETLLT
                     GGALAELSRHALDYARSTDLYSMHERAADVIEQVANRL"
     gene            complement(1853..2509)
                     /locus_tag="PKI68_06445"
     CDS             complement(1853..2509)
                     /locus_tag="PKI68_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="toluene tolerance protein"
                     /protein_id="HNX53366.1"
                     /translation="MRTLSKDEYEALLAQSEIIEKDRHGVKVLRLPDGNFLKTFWFRR
                     LISSRRIYPESFRFMINAKALRRRGIPTVLVRKRVQIPHLNRTGVIYQPLAGRTLRQV
                     AKDGQFTAQLAGLLGAFIAGLHRQGVYFRALHLGNVVLCPDGTLGLIDISNLTAYPWP
                     LGQRAGMRNLFHLFRYPNDSQVIADAGTRNFMDGYLKEKFCPRMQRRMRRKIKRWVAE
                     "
     gene            complement(2550..4316)
                     /locus_tag="PKI68_06450"
     CDS             complement(2550..4316)
                     /locus_tag="PKI68_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX53367.1"
                     /translation="MKKAAFKIPERIRIFAPYFAMLKPVKWQFSGAILFALLNGATNG
                     FGVPVIVYKVLPKVFASGQPDGWMLAGVVVLFPLIGLVRGASDFFSSYLSAVCGVRVL
                     IQIQSRLYRKLQELPLGFYGKMRIGDLMSRVMGDTAAVQTVVTNNVADLVRQPVQFIG
                     AIAALVYISIQKKEAVFILFALAVVPLCIMPIRYFGKRMKARAKKLQAGAGSLSTAIN
                     ENLGAVREVRAFNLQELETAKFAERMSVIAKFHLKKTKYSKMLPPLIELIGICFVGLG
                     VLYCARVHVTLEDMVPLLLALYMTYPSIRKFGTVHNDIRQGEASVERIEQVMQTPDAV
                     PDPAVPVPFNAKNTDIEFENVGFEYEAGVPVLCQVNAQIPSGTVVALVGPSGAGKSTF
                     VNLIPRFYDVTGGAIRIGGIDLRQFSKIDLRNHMAIVSQDTILFNDTIRNNIRLGRQD
                     ASDAEVEQAARHAFAHDFITAFDEGYNTLVGERGTRLSGGQKQRIAIARAFLRNAPVL
                     ILDEATSALDSESEEMVQKALVELVKGRTVFIIAHRFSTIKLANRIMVFERGILRSYG
                     SHPELYASDELYKSLYDRQFIE"
     gene            complement(4370..5356)
                     /gene="waaF"
                     /locus_tag="PKI68_06455"
     CDS             complement(4370..5356)
                     /gene="waaF"
                     /locus_tag="PKI68_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HNX53368.1"
                     /translation="MKPENPERILIVSTNWIGDAIMSMPAVQLFRRAHPEAELTVLAR
                     PLIRALWAMHPAVDRLETMGKPQPTVQSLKPAHFDRAYIFPNSFRSAFIPFLAGVPRR
                     IGARGHWRRLMLTETVRLPDGHQQFEYLKILGVQGEPPAPQLAVPEESFNSLNNRLAA
                     AGFRPACRTVITLLPGAARGPSKRWPAEHFIVLAKRLRAELGAQILLGGGPDDAAVCA
                     EIAAAVGPEAISLAGQTTVAEWAALLKMSQCVVSNDSGGMHLAMAVGTPVAAIFGITD
                     SRKTGPLGRSVVLQKSAVQSRDIARDSEAARRALEAVTPDEVFAAVTGLLNG"
     gene            complement(5353..6501)
                     /gene="lpxK"
                     /locus_tag="PKI68_06460"
     CDS             complement(5353..6501)
                     /gene="lpxK"
                     /locus_tag="PKI68_06460"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNX53369.1"
                     /translation="MPTRSNERLEQRLLKVISKEVNGKRAACLRGFLKSLSWIFVALV
                     QLRLLLHRHRIIRASTLGCQVISVGNITVGGTGKTPIVETFARSLQQKGRKVAILSRG
                     YKSRKTPLWEKILKKEERLPRVVSDGQRLLLNSDLAGDEPYMLASNLPDAVVLVDKDR
                     VKSGKYAIKKFGCDTLVLDDGFQYLKLQHRLDIVLVDYTNPFGYRRVLPRGLLREPMR
                     NLKRAGFIFITKCPPEGAPELKARLRELNPRAEISECRHSPKYLENLYTREQTGLDFL
                     QGKKIAAVSGIAVPESFENGLRKLGAEIVHSARYADHHRYTQQEIIETINQAAEAGAE
                     VIVTTEKDAVRFPLLDRCDLPVIFLRVEIEMLSGSEAFNDWIDRICFK"
     gene            complement(6510..7283)
                     /locus_tag="PKI68_06465"
     CDS             complement(6510..7283)
                     /locus_tag="PKI68_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX53370.1"
                     /translation="MTLKLCVLASGSSGNCTFIGTGNTRILIDAGLSARKTAERLADI
                     GERVEDITAICVSHEHGDHIAGIRVLQKNYGIPVYANAGTFEIFGGDIKGSRFTTGAP
                     FSIGDFSIHPFPVPHDANDPVGFIFSAGSLMVGVATDIGMVTNALRERLRKCRAVVIE
                     ANHDETLLHEADRPWSLKQRIRGNQGHLSNRAAAALMAEIAGEGLQHLFLAHLSADCN
                     SPQHARKTFETLLAEAGHTHVNVRLTCAECVSEQLILEG"
     gene            7374..>8761
                     /gene="rpoN"
                     /locus_tag="PKI68_06470"
     CDS             7374..>8761
                     /gene="rpoN"
                     /locus_tag="PKI68_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNX53371.1"
                     /translation="MAEQYLGHIQEQRMEQSLTPQMLQSLKILQAPILDLQTMIREEM
                     DQNPTLEREAPEVEQTTDFDAAAREELREEREIGELTELAEWDADFRSNREVRSQSDD
                     EQHQFMMDSIEGAASLQEHLLEQLALAGLDARERGIAEVLIGSIDDDGYLQLDLDGII
                     GSTPEFPEELFERIIKVIQGFDPVGVGARDLKECLLLQLRHKGRENSLEAVLVRDHLD
                     KLGAHQYEQIARAMRLPIEKIKELSAAVADLDPKPGRNFTADKTEYIIPEITVEKKGG
                     IWTVTQNKSPYPRLFISQKYLELLKDKTTAADTRRYIREKIAKSKFFIRSIDQRQDTI
                     YRLACEIVRVQEDFLEEGVSGLKPLTMKQVADILEVHETTVSRACSGKYMATPQGTFE
                     FKYFFTTGVVQADGRILSNASIKSALASIVRTESSRRPLSDQRIADELSKQGIDIARR
                     TVAKYREQLKILP"
BASE COUNT         1839 a         2726 c         2511 g         1685 t
ORIGIN      
        1 ggcccgccag aatccggcgc cgttgcgcag cgtttcgcgc agcgaaccgc cggaatagat
       61 ttccataaag cgcaggcggt cccgtgacgt caacccgata ttcatggccg aaaaatagag
      121 gccggccaca tccttgaccg cccagcggcg cggcgtcttt ttacgcagct gtgcgcggtg
      181 cagatcaatc agcgaggctt tgatgccggc cttccagtcc cgggaaacat ccagaagaaa
      241 gtggcagagg taatagtcgc ggtggttcac gccgttgcag tgcagcaggc gactgacctg
      301 cgccacatag cggatcagcg cgagcttcag ctcaaacggc ggcggattcc gtttccagtc
      361 gcggcagtag tcttcgaggc tgacggtatt caccagctcg tcggtgatga tgaaggattc
      421 gatccgggcc ggattgcggc cgcgctcgcc gaaggccgcg atcttcatcg tcgccacgcc
      481 cagcttttcg agccgccgga ttgcttcgta ctcattgcgg gcgctgaaaa ccggccgttt
      541 cagctgcagc aggtttttta aaatctcggc ccagccgatg ccgtgatgca ctttggcaaa
      601 gtagcttttc ccgccgagtt cgaaccggaa ggtgcgccgg gtcttcaccg accggaacac
      661 ttcgccttcc agccggaagg ccgctgcgaa cggatccgcc gcagcccagg cttttttaaa
      721 atgggtattg agatccatca tagccggttc gccacctgtt caatcacatc cgccgcccgt
      781 tcgtgcatgc tgtagagatc ggttgagcgg gcataatcca gtgcatgccg gctgagctcc
      841 gccaaagcgc cgcccgtcag cagcgtttcg agctgatggt tcagcacggc ctgatcgaac
      901 ggttccggcg tcagcagtcc gcactgcgca gtccggatgt gactgccgta gccgcagact
      961 tcggtgcaaa gcaccggcag accggcggcc accgcttcca gcagaaccgt tccggtattt
     1021 tcgtcatagg ccggatggat cattaaatcg gcgcccagca gaatctgcgg aacatcgtcg
     1081 cgcccgccgg tgaaaatcac gcgcccgccg acgccgaggt cgcgggccag cttctcaaag
     1141 cgcgcggcgc ggtctttgcc gaccacccag agaaccgtcc gctcccggac gtccgggggc
     1201 agcgcggcca gcgcacgaat ggagcggtcc accccctttt tgataaagcc ggagccgatc
     1261 tgcaccagca gccgggtttc gtcgctcagt ccgtgggccc ggcgaaaggc ggcccgcagt
     1321 ttcggcgcat cgggcggcgc gatgcggtcg cgcgcaatgt tcggcggcag aaagtgcatc
     1381 cgctccggct gcgttccgta gtgcttcaca aagaacggtt cctgggtttt cgaaatcatc
     1441 agcagctcgg ttgcagagtc tttgccgaac accgcttttt caaacgcgct atagagccgg
     1501 tagcgcgccc cgagccggta gaacggcccg cgctgcgtca gcgatttttc tttatagcag
     1561 gcgtccgccg cataatagac gtcgaggccg ggcattttat tgaagccgac ccgcacgctt
     1621 cccgctgcgg cgtttttctc cacccattga aaatattttt ttgcgcggcg gtggttgttc
     1681 agggcgcgga ccggcaccag gcagacatca aatccgggcg gcaccgggcc gtcccaacta
     1741 agggtgtaca ccgcaatcga atatccgcgc cgctggcatt cgagtgcgat ccgcagaaaa
     1801 tcgcgctgaa gcccgccgta gggaaaatat ttaaacaggc agaaggcaat tgtcattcgg
     1861 ccacccaccg tttgattttt cggcgcatcc ggcgctgcat gcgcggacag aatttttctt
     1921 tcagataccc gtccataaaa ttacgggtcc ccgcgtcggc aatcacctgc gagtcgttcg
     1981 gatagcggaa cagatggaac agattgcgca tgccggcgcg ctggccgagc ggccacggat
     2041 aggcggttaa gttggagatg tcgatcagcc cgagggttcc gtcggggcac agcacgacat
     2101 tgccgaggtg cagcgcgcgg aaataaacgc cctggcggtg gagtccggcg atgaaggcgc
     2161 ccagcaggcc cgccagttgc gcggtgaact gcccgtcctt ggccacctgc cgcagcgtcc
     2221 gcccggccag cggctggtaa atcacgccgg tccggttgag atgcggaatc tgaacccgct
     2281 tgcgcaccag aacggtcgga attccgcggc gccgcaaggc ctttgcattg atcatgaaac
     2341 ggaacgattc cgggtagatc cggcgggagg aaatcagccg gcggaaccag aaggttttca
     2401 gaaaatttcc gtccggcagc cggagcactt tgaccccgtg ccggtccttc tcgataattt
     2461 ccgactgcgc aagcagtgct tcgtattcgt ctttggacag tgttcgcatt ctggaaaacc
     2521 ccgggcctta acggaaccgt gcaggccgtt tactcaataa actgccggtc atagaggctc
     2581 ttgtagagtt cgtcggaagc gtacagttcc gggtgtgaac cgtagctccg cagtatgccc
     2641 cgctcaaaaa ccatgatgcg gttcgccagc ttgatggtgc tgaaccggtg ggcgatgatg
     2701 aagaccgtgc ggcctttcac cagctcaacc agtgctttct gcaccatctc ttcactctcg
     2761 gaatccagcg ccgaggtggc ctcatccaga atcagaaccg gcgcgttgcg caggaacgcg
     2821 cgggcaatgg cgatgcgctg tttctgcccg ccggacaacc gcgtaccgcg ttcgccgacc
     2881 agcgtgttat agccttcatc gaacgccgta ataaagtcgt gggcgaaggc atgccgcgcc
     2941 gcctgctcca cttcggcatc gctcgcatcc tgccgtccga gacggatgtt attgcgaatg
     3001 gtgtcgttaa acagaatcgt atcctgcgat acgatcgcca tgtggttgcg caggtcgatt
     3061 ttgctgaact ggcgcagatc gatgccgccg atgcggatcg cgccgccggt cacgtcgtag
     3121 aaacggggaa tcagattcac aaaggtggat ttaccggcgc cgctcggccc gaccaaagcc
     3181 acaaccgtac ccgacggaat ctgcgcattc acctggcaca gcaccggcac gccggcttcg
     3241 tattcaaagc cgacgttttc aaattcaatg tcggtattct tcgcattgaa cggcaccgga
     3301 accgcaggat ccggcaccgc atccggcgtc tgcatcacct gttcaatacg ttcgaccgac
     3361 gcttcgccct gccggatgtc gttatgcacc gttccgaatt tgcggatcga cgggtaggtc
     3421 atatagagcg caagcagcag cggcaccata tcttccagcg tcacatgaac ccttgcgcag
     3481 tacagaacgc cgagtccgac aaaacagatg ccgatcagct cgatcagcgg cggaagcatt
     3541 ttggaatact tggttttttt gagatgaaat ttggcgatca cgctcatccg ttcggcgaac
     3601 ttggccgtct cgagctcctg caaattgaaa gcccggactt cgcgaacagc tcccaggttt
     3661 tcattgattg ccgtcgacag gctgccggcg ccggcctgca gttttttggc ccgcgccttc
     3721 atgcgcttgc cgaaatagcg gatcggcatg atgcaaagcg gcaccacggc cagtgcaaag
     3781 agaataaaaa ccgcctcttt cttttggatg gaaatataaa cgagtgccgc aatggccccg
     3841 ataaactgaa ccggctgccg gaccagatcg gcgacgttgt tggtgaccac ggtctgtaca
     3901 gcggccgtgt cgcccatcac gcgggacatc aggtcgccga tacgcatttt cccgtagaaa
     3961 cccagcggaa gctcctgcag cttgcggtag agtctgctct gaatctggat cagcacccgc
     4021 acgccgcaca cggcggaaag ataggagctg aaaaaatcgc tcgcgccgcg caccagcccg
     4081 atcaacggaa agagcacaac cacgccggcg agcatccagc cgtccggctg ccccgaagca
     4141 aaaacctttg gaagcacctt ataaacaatc accggcacac caaagccgtt cgtggcacca
     4201 ttcagcagag caaacagaat tgcaccggaa aactgccatt tcaccggttt gagcatggcg
     4261 aaatacggcg cgaagatccg gatccgttca ggaattttaa aagcggcttt tttcatagag
     4321 ggtggttcta tcatgaaaca gccgaaggct ccaagccttt cgaccggttt cagccattca
     4381 gcagaccggt cacggcggca aaaacctcgt ccggcgtaac ggcttccagc gcccggcggg
     4441 ccgcttccga atcgcgggcg atgtcgcgcg actgcacggc gcttttctgc agaaccaccg
     4501 accggccgag cggcccggtt tttctggaat ccgtaattcc gaaaatcgcg gcgaccggcg
     4561 tcccgacggc catcgccaga tgcatcccgc cgctgtcgtt cgacaccacg cactgactca
     4621 ttttcagcag cgccgcccac tccgcaaccg tggtctggcc ggccagactg attgcttccg
     4681 gccccaccgc ggcggcgatt tctgcgcaaa cggccgcgtc atccgggccg ccgcccagca
     4741 gaatctgtgc gcccagttcc gcgcgcagcc gtttcgccag cacaataaaa tgctccgccg
     4801 gccagcgctt cgacggtccg cgcgccgcgc ccggcagaag cgtaatgacc gtccggcagg
     4861 caggcctgaa tcccgcagcc gccagccggt tattcagaga gttgaaactt tcttccggca
     4921 cggccagctg cggcgccgga ggctcgccct gcacgccgag aattttcaga tattcaaact
     4981 gctggtgacc atccggcaga cggaccgttt ccgtcagcat cagccgccgc cagtggccgc
     5041 gggcgccgat gcgccgcggc acgcccgcca ggaacgggat gaatgccgag cggaaggagt
     5101 tgggaaaaat ataggcgcgg tcgaagtgcg ccggtttgag gctctgcacc gtgggctgcg
     5161 gcttccccat ggtttcgagc cggtcgacgg ccgggtgcat ggcccacagc gcgcggatca
     5221 gcggccgcgc cagcaccgtc agttcggcct ccggatgcgc ccgccggaac agctggacgg
     5281 cgggcatgct cataatcgca tccccgatcc agttggtgct gacgatcaat attcgttctg
     5341 gattttcagg cttcatttaa aacagatccg gtcgatccag tcgttgaagg cttcggaccc
     5401 ggagagcatt tcaatttcaa cgcgcaggaa gatcaccggc aggtcgcagc ggtccagcag
     5461 cggaaagcgc acggcgtctt tttcggtggt gacgatcact tcggccccgg cttcggcggc
     5521 ctgattgatg gtttcaatga tctcctgctg ggtgtagcgg tgatggtcgg cgtaccgcgc
     5581 gctgtgaacg atttcggcgc cgagcttgcg gaggccgttt tcaaaactct ccggcacggc
     5641 aataccggag acagcggcga tctttttgcc ttgcaggaaa tcgagcccgg tctgttcgcg
     5701 ggtatacagg ttttccagat atttcgggct gtggcggcat tcggagattt cggcgcgcgg
     5761 attaagttcg cgcagtctgg ccttcagctc cggcgcacct tccggcgggc atttggtgat
     5821 aaagatgaat cctgcccgct tgagattgcg catcggttcg cgcagcagac cgcgcggcag
     5881 gacgcgccgg tagccgaacg ggttggtgta gtccaccagc acgatgtcga gccggtgctg
     5941 aagcttgagg tactggaacc cgtcgtcgag caccagcgta tcgcagccga attttttaat
     6001 ggcgtacttc ccggatttca cgcggtcttt atccaccagc accacggcat ccggcaggtt
     6061 ggaggccagc atgtacggct cgtcgccggc caggtcggaa ttcagcagca ggcgctggcc
     6121 gtcagacacc acgcgcggca ggcgttcttc tttcttgaga atcttttccc acagcggcgt
     6181 tttccggctc ttataaccgc ggctcagaat ggcgacttta cgccccttct gctgaagcga
     6241 gcgcgcaaag gtttccacaa tcggcgtctt gccggttccg ccgacggtaa tgttgccgac
     6301 gctgatcacc tgacagccga gtgtgctggc gcggataatc cggtgccggt gaagcagaag
     6361 ccgcagctgc accagcgcaa caaaaatcca tgaaagggat ttcagaaaac cgcgcaggca
     6421 ggcggcgcgt tttccgttga cctctttcga gatcaccttg agcagtctct gttcaagccg
     6481 ttcgtttgag cgggtcggca tactaaatct tatccttcta aaatcagttg ttcgctgacg
     6541 cattcggcgc acgtcagccg cacgtttaca tgcgtatggc ccgcctcggc cagcagggtt
     6601 tcaaaggttt tccgagcgtg ctgcggactg ttgcaatctg cgctcaggtg cgcaagaaaa
     6661 agatgctgca acccttcgcc ggcgatttcg gccatcagcg ccgccgccgc ccggttggaa
     6721 aggtgtccct ggttgccgcg aatgcgctgt ttaaggctcc atggacggtc ggcttcgtgc
     6781 agaagcgtct cgtcatggtt ggcttcgatc accaccgcac ggcatttacg cagccgttcg
     6841 cgcagcgcat tcgtcaccat gccgatatcg gttgcgacgc cgaccatcag gctgcccgcc
     6901 gagaaaatga atccgacagg atcgttggcg tcgtgcggta ccggaaacgg atggatggaa
     6961 aagtcgccga tggaaaacgg cgcgccggtg gtaaagcgcg agcctttgat atcgccgccg
     7021 aatatttcga aggttccggc gttggcgtag accggaatgc cgtagttttt ctgaagtaca
     7081 cggataccgg cgatatgatc gccatgctcg tggctcacgc agatggcggt aatatcctcc
     7141 acccgctcgc cgatatccgc cagccgttcg gccgttttac gggccgaaag ccccgcatca
     7201 atcaggatgc gagtgtttcc ggtgccgata aacgtgcagt tgccggaact gccgctggcc
     7261 agtacacata gtttcaaggt catacagtta aaattggatg ttacagccga agctgttctg
     7321 tgttattggt aagcgcgtaa ttgttagaca ggacggggag tttgtaaatc caaatggcag
     7381 aacagtatct aggacatatc caggagcagc ggatggagca gtcgctcact ccgcagatgc
     7441 tccagtccct gaaaatcctc caggcgccta tccttgacct ccagaccatg atccgcgagg
     7501 aaatggatca gaatcccacg ctggagcgcg aagcccctga agtcgagcaa accaccgatt
     7561 tcgacgccgc cgcccgcgaa gaactgcgcg aagaacgcga aatcggcgaa ctgaccgagc
     7621 tggccgagtg ggacgcggat ttccgcagca accgcgaagt ccgctcgcag agcgacgacg
     7681 aacagcacca gtttatgatg gattccatcg aaggcgcagc ctcacttcag gaacacctcc
     7741 tggaacagct tgccctcgcc gggctcgacg cccgcgagcg cggcatcgcc gaagtcctga
     7801 tcggaagcat cgacgacgac ggctacctcc agctcgacct cgacggaatc atcggctcca
     7861 ccccggaatt tcccgaagaa ctgtttgagc gcatcatcaa agtcatccag ggattcgatc
     7921 cggtcggcgt cggcgcgcgc gacctgaaag aatgcctgct gctccagctc cgccataaag
     7981 gccgggaaaa ctccctcgaa gccgtccttg tgcgcgacca cctcgacaag ctcggcgccc
     8041 accagtatga acagatcgcc cgcgccatgc gcctgccgat cgaaaaaatc aaagaactct
     8101 ccgccgccgt tgccgatctc gacccgaaac ccgggcgtaa ctttaccgcc gataaaaccg
     8161 aatacatcat cccggaaatc accgtggaga aaaaaggcgg catctggacc gtcacccaga
     8221 acaaaagccc ctatccgcgc ctcttcatca gccagaaata cctcgaactg ctcaaagaca
     8281 aaaccaccgc cgccgacacc cgccggtata tccgcgaaaa aatcgcgaaa agcaaattct
     8341 tcatccgcag catcgaccag cggcaggaca ccatctaccg cctcgcctgc gaaatcgtcc
     8401 gcgtacagga agatttcctc gaagaaggcg tctccggcct caaaccgctc accatgaaac
     8461 aggtcgccga catcctggaa gtccacgaaa ccaccgtcag ccgcgcctgc agcggaaaat
     8521 atatggccac tccgcagggc actttcgaat tcaaatactt cttcaccacc ggcgtcgtcc
     8581 aggccgacgg acggatcctc tccaacgcct ccatcaaaag cgccctcgcc tcaatcgtcc
     8641 gcaccgaaag cagccgccgc ccgctttccg accagcggat cgctgacgaa ctgagcaaac
     8701 agggaatcga catcgcccgc cgcaccgtcg ccaaataccg cgaacagctc aaaattctcc
     8761 c
//
LOCUS       DAOPZO010000084         8383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_10138_length_8383_cov_8.690562, whole genome shotgun sequence.
ACCESSION   DAOPZO010000084 DAOPZO010000000
VERSION     DAOPZO010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 8383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8383
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10138_length_8383_cov_8.690562"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1498
                     /locus_tag="PKI68_06475"
     CDS             <1..>1498
                     /locus_tag="PKI68_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNX53372.1"
                     /translation="YKNYILVCAGTACESNKGKALYEALEKEIKAAGVEAEAQLVKTG
                     CFGFCEQGPIVKILPDESFYVKVGPKDAHEIIAEHIVKGRKVERLLYKENKAENDPRS
                     SDISFYRKQLRIVLRNCAVINPEIIDEYIARDGYAALEKVLFEMTPDAVIKEIVDSGL
                     RGRGGAGFPTGRKWQFTKNTPADQKYIVCNADEGDPGAYMDRSVLEGDPHSVLEAMTI
                     AGYTVGASKGFIYIRAEYPLAIDRLNTAMAQAREYGLLGDRILGTDFSFDIEIRLGAG
                     AFVCGEETALLASIEGNRGMPVPRPPFPAVKGLWGKPTVINNVETYANIPVILLKGSK
                     WFSSIGTETSKGTKVFALTGKINNSGLIEVPMGTTLRDIIYDIGGGIASGKKFKAAQT
                     GGPSGGCITAAFLDTPIDYESLQSIGSMMGSGGMIVMDEDDCMIDVTKFYLEFTVDES
                     CGKCAPCRIGGRTCYNILDRISKGQGKPDDIQKLKEIGHAMRKASLCGL"
     assembly_gap    1499..1598
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1599..3350
                     /locus_tag="PKI68_06480"
     CDS             <1599..3350
                     /locus_tag="PKI68_06480"
                     /inference="COORDINATES: protein motif:HMM:NF040763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HNX53373.1"
                     /translation="PMNMIELEINGIPVSVKPGTSLIDAAKLVGVKIPTLCYHADLDP
                     WAACGICVVKVEGSPKMLRACATPAGPGMKVITHDPEIVNVRRTVLELILSTHPNDCL
                     QCSRNRNCELQTLAEEFGIREVSYERRLRHLPADDSTKSVVLNPEKCIACGRCAKVCQ
                     NMQGVWAIEFIGRGETTRIAPAADVVLDESPCIKCGQCTAHCPVGALYEHDQTREVWN
                     AIEEHKKHCVVQIAPAVRVAIGEAFGYEPGEVLTGQLYAALRRLGFNAVFDTNFTADL
                     TIMEEGSEFVHRVTEGGVLPQITSCCPAWTDYMEKFAPDFIPNFSSAKSPQQMLGAMA
                     KTYYAEKAGIDPKDMYVVSVMPCTAKKFEVSRDKGMTSSGFQDVDVSITTRELARMIK
                     QAGIDFKNLPNEEPDSLLAEYSGAGTIFGVTGGVMEAALRTAYFLITKEDLKDVHFMD
                     VRGLEGVKAATVDVKGTKVRVAVAHQMGNVEAVLNQVRQAKEKGEELPYHFIEVMACR
                     GGCIGGGGQPHGATDEIRKKRMAGIYTDDEKSVLRCSHHNPMIKKIYEDFLGKPLSHK
                     AHELLHTGYQERKVYKK"
     gene            3506..3757
                     /locus_tag="PKI68_06485"
     CDS             3506..3757
                     /locus_tag="PKI68_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HNX53374.1"
                     /translation="MQKRLGFIGIIIENREANAAAVNELLGDFGDIIVARMGVPYEKR
                     ACSAITLIVDTTTDELGMLTGKLGALPGISVKSMLSKAK"
     gene            3754..5082
                     /locus_tag="PKI68_06490"
     CDS             3754..5082
                     /locus_tag="PKI68_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNX53375.1"
                     /translation="MKTRIETDFLGSKELPADVLWGIHTARAMENFQIPGQSVNPRLL
                     TAYVQVKKACALANKELGFLSAEKAEAIITTCDQFHLSSFIPHLSSLQGGAGTSTNMM
                     VNEVIGNLAGAHPIEEVNLHQSTNDTYPTALKIAAIVGVRELSEAFAALQGTFQTLEK
                     TWAHLPILGKTELMDAVPLTLGTQFSSFAEAFARDRWRTFKCEERLRTVNLGGTAIGT
                     GITAPRSYIFLVTEKLREVTGLGLCPAENRVEATANTDCFVEVSGILNAAAVNLIKVS
                     DDLRRLAMLGEIRLPAVQAGSSIMPGKVNPVILESVMQIGIKVKANSNIVADCSARGS
                     LQICEFMPLLADALLESIGLLIAAAQMLAKHAAGIDADQGKCLERLYASPEIITAFVP
                     LLGYDRCSELFKQFEQHGKGNIRGFLEKELGKETVEKTLSPQNLISLGHK"
     gene            5079..6290
                     /gene="hydF"
                     /locus_tag="PKI68_06495"
     CDS             5079..6290
                     /gene="hydF"
                     /locus_tag="PKI68_06495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HNX53376.1"
                     /translation="MKTAPKSLRLHIALFGRTNVGKSSFLNLIAGQDVSIVSEQPGTT
                     TDVVEKTMELLPIGPVVFIDTAGLDDVTALGGRRIEKTEKVFDRADVILLICEGSRFG
                     EFEKAVETRAVQKNVPVIRILNKSDLYEVSDLSDLCVNSEDLSSREKVLSQLKARLIS
                     VCPEEFIQPPPLVGDLVKPGGIAVLIVPIDLQAPKGRLILPQVSTIRDALDNDAAALV
                     VKEREYAHMLGRLKTPPDLVVCDSQVVLKMIGDTPNGIPCTTFSILFARLKGDLPKMA
                     AGAAAIDRLKDGDKVLIAESCSHHAAEDDIGRVKIPRWLRQYCGCELQIDIYAGRDFP
                     DNLSEYSLVVQCGGCMHNRREILSRIEKCEAAGVPITNYGLCISQTQGVLKRVLSPFP
                     AALDAFERNLK"
     gene            6287..7693
                     /gene="hydG"
                     /locus_tag="PKI68_06500"
     CDS             6287..7693
                     /gene="hydG"
                     /locus_tag="PKI68_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HNX53377.1"
                     /translation="MKIDTEGLKSFIPEAEIFQWLEKTKNPSAERVREIIAKSLNKNR
                     LDPEEMAVLINAEDPALADEIFEGARELKKRIYGNRIVLFAPLYVGNECINNCQYCGF
                     RCTNKEVSRKTLTMPELDCEIEALVTRGHKRLILVYGEHPQYDAQFIHDTVKEVYARK
                     HGNGEIRRVNINAAPLDVEGYRLVKSAGIGTYQIFQETYHQETYKQMHPSGPKSSYLW
                     RLYGLDRAQEAGIDDVGIGALMGLFDWRFETMALLYHTIHLEEKFHVGPHTISFPRIE
                     PAIGTAMADHPPSGVSDYDFKRLVAILRLSVPYTGLILTCRESVAIRNEIIQFGVSQI
                     DAGSDIGVGAYSQNKESYKKSQFVLNDGRSLDQVIRELCEADFIPSFCTGCYRLGRTG
                     EHFMEFAIPGFVKRFCTPNAVLTFLEYMEDYSSPETKKAGMKQIERELERMPDGEQKT
                     QLLERMAQIKQGNRDLYF"
     gene            7713..>8383
                     /locus_tag="PKI68_06505"
     CDS             7713..>8383
                     /locus_tag="PKI68_06505"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX53378.1"
                     /translation="MTYAILEKVDRDEELTPAEMKALLEVADPAELQALYDCAYRVKA
                     KYVGKVAYFRGIIECSNICIKDCYYCGIRKSNTNVKRFQMDEEEMVREAVWAFENEYG
                     SVVIQSGERQDAAYIELIERVLRRIKTDTDGKLGVTLSLGEQTEETYRRWREAGAHRY
                     LLRIETTNPALYRKLHPADHDFEVRKNCLSLLKKTGFQVGTGVMMGLPGQTMDDLVND
                     ILFMK"
BASE COUNT         2036 a         2389 c         2216 g         1642 t
ORIGIN      
        1 catacaaaaa ctacattctg gtatgcgcgg gaaccgcgtg tgaatccaac aaaggcaaag
       61 ctctctacga ggcgcttgaa aaagaaatca aagcggccgg cgtcgaggct gaggcccagc
      121 tggtgaaaac cggctgcttc ggtttctgcg aacagggtcc gattgtcaaa atccttcccg
      181 acgaaagctt ctacgttaag gtcggtccga aagacgcgca cgaaattatt gccgaacaca
      241 tcgtcaaggg ccgcaaggtc gagcgtctgc tctataaaga aaacaaagcg gaaaacgatc
      301 cgcgttccag cgacatcagc ttttaccgca aacagctgcg catcgttttg cgcaactgcg
      361 ctgtcatcaa tccggaaatc atcgacgaat acatcgcccg cgacggatat gccgcgctcg
      421 aaaaagttct tttcgaaatg acaccggatg cggtgattaa agaaatcgtc gattccggtc
      481 tgcgcggccg cggcggcgcc ggcttcccga caggacgcaa atggcagttc accaaaaaca
      541 cgccggccga ccagaagtac attgtctgta acgccgacga aggcgatccg ggcgcctaca
      601 tggaccgcag cgttcttgaa ggcgatcccc actctgtact cgaagccatg acaattgccg
      661 gatacaccgt cggtgccagc aaaggattca tctatatccg cgcggaatat ccgctggcca
      721 ttgaccggtt gaacaccgcc atggcgcagg ccagagaata cggactgctc ggcgacagga
      781 ttctcggcac cgatttcagc ttcgacatag agatccgtct gggcgccgga gcgtttgtct
      841 gcggcgaaga aaccgccctg ctcgcctcaa ttgaaggcaa ccgcggcatg ccggttcctc
      901 gtcctccctt cccggccgtc aaaggcctgt ggggcaaacc gaccgtcatc aacaacgtcg
      961 aaacctacgc caatatcccc gttattctgc tcaagggcag caaatggttc agcagcatcg
     1021 gcacggaaac atcaaaggga accaaggtgt tcgcgctgac cggcaaaatc aacaactccg
     1081 gtctgattga agtcccgatg ggaacgaccc tgcgcgacat catctacgat atcggcggcg
     1141 gtattgccag cggcaagaaa tttaaagccg cgcagaccgg aggcccgtcc ggcggatgta
     1201 ttacggcagc attcctcgat acgccgatcg actacgaaag ccttcagtcc atcggctcga
     1261 tgatgggatc cggcggtatg attgtcatgg acgaagatga ctgcatgatt gacgtcacca
     1321 agttctacct tgaattcaca gtggatgaat cgtgcggtaa atgtgcaccc tgccgtatcg
     1381 gcggacgcac atgctacaac atcctcgacc ggatcagcaa aggtcaaggg aagccggacg
     1441 acatccagaa actcaaggag atcggccacg ccatgcgcaa agcctcgctt tgcggcctnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc catgaacatg attgaactcg
     1621 aaatcaacgg aattccggtg tcggtaaagc cgggcacctc gctcattgac gcggctaaac
     1681 tggtcggcgt taaaatcccg acactttgtt atcacgcaga cctggacccg tgggcggcct
     1741 gcggcatctg cgtcgtcaaa gtcgaaggct cgccgaaaat gctgcgcgcc tgcgccactc
     1801 cggcaggtcc cggcatgaag gtgatcaccc acgacccgga aattgtcaac gtgcgccgta
     1861 cggtgcttga actgattctt tccacccatc ccaacgactg tcttcagtgc agccgcaacc
     1921 gcaactgcga attgcaaacg ctggccgaag aattcggtat ccgcgaagtg tcttacgaac
     1981 ggcgtctgcg gcacctgcct gcggacgact cgaccaaatc cgtcgtgctt aatcccgaaa
     2041 aatgtatcgc ctgcggacgt tgcgctaaag tatgccagaa catgcagggt gtatgggcga
     2101 ttgaattcat cggacgggga gaaaccaccc ggattgcgcc ggcggcggac gtcgtattgg
     2161 acgaaagccc ctgcattaaa tgcggtcagt gcactgcgca ctgtcccgtc ggtgcgcttt
     2221 acgagcacga ccagacccgt gaagtttgga atgcgattga ggaacacaag aagcattgcg
     2281 ttgtacagat tgccccggcc gtgcgtgttg ccatcggcga agccttcggc tacgagcccg
     2341 gcgaagtact taccggacag ctttatgccg cattgcgccg tctcggtttt aatgcggtct
     2401 tcgacaccaa cttcaccgcc gaccttacca tcatggaaga aggttcggag tttgtgcacc
     2461 gcgtgaccga aggcggagtt ctgccgcaga ttacctcctg ctgtccggcc tggacggatt
     2521 acatggaaaa attcgcaccc gacttcatcc cgaacttctc ttcggccaag tcgccgcagc
     2581 agatgctcgg agcaatggcg aaaacgtatt acgccgaaaa agcgggtatt gacccgaaag
     2641 acatgtatgt cgtttccgtc atgccttgca ccgccaagaa gttcgaagtc tcccgagaca
     2701 aaggaatgac ttcgtccggc ttccaggacg tggatgtgtc catcaccacc cgcgaactgg
     2761 cacgcatgat caaacaggcc ggtatcgact tcaaaaacct gcctaacgaa gaaccggaca
     2821 gtctgctcgc cgaatattcc ggcgcaggca cgattttcgg tgtaaccggc ggcgtcatgg
     2881 aggctgcgct tcgcacggct tacttcctta ttaccaagga agaccttaag gatgtccact
     2941 ttatggatgt ccgcgggctg gaaggcgtaa aagccgccac agtcgatgtc aaaggtacta
     3001 aagtccgcgt cgcagtcgcc catcagatgg gtaacgtcga agccgtgctt aatcaggtac
     3061 gtcaggcaaa ggaaaagggc gaagagctgc cctaccactt catcgaagtc atggcctgcc
     3121 gcggcggttg tatcggcggc ggcggacagc cccacggcgc gaccgacgaa atccgcaaga
     3181 aacggatggc cggaatttac acagatgacg aaaagagcgt cctgagatgc tcccaccaca
     3241 acccgatgat caagaaaatt tacgaagatt ttcttggtaa accgttgagc cacaaggctc
     3301 acgaactttt gcacaccggc tatcaagaac gaaaagtgta caaaaaatag aaacgggtgt
     3361 actgttttcg ggttttctgc ggcaaggggc ctttccctga cccaaggcga acggacaatt
     3421 taggaaaacg gtttgcatag ctgcctaccg tctccatgct ccgccgcaga aatgcggcgg
     3481 agcacttttt ttaggaggaa cggctatgca aaagagactg ggttttatcg gaatcatcat
     3541 tgagaaccgc gaagcgaatg ccgcagcggt caacgaactg ctgggcgact tcggcgacat
     3601 cattgtcgcc cgcatgggag tcccttacga gaagcgcgcc tgctccgcca tcacccttat
     3661 cgtcgacacc acgactgacg aactcgggat gctcaccgga aaactcggcg ccctgcccgg
     3721 catctccgtc aaatccatgc tgagcaaagc caaatgaaaa cccggatcga aacagatttc
     3781 ctcggttcaa aggaacttcc tgcggatgtc ctgtggggaa ttcataccgc tcgcgccatg
     3841 gaaaattttc aaattccggg gcaatctgtt aacccgcgtc tgctcacagc atacgttcag
     3901 gttaaaaaag cctgcgccct cgccaataag gaactcggct tcctttccgc cgaaaaagct
     3961 gaggcgatca tcaccacctg cgaccaattt catctctcat ctttcatccc tcatctttca
     4021 tctcttcagg gcggagccgg aacttcaacc aacatgatgg tcaatgaagt tatcggaaat
     4081 ctcgccggag ctcatccgat cgaagaggtc aatctgcacc agtccaccaa tgacacctac
     4141 ccgactgctc tcaagattgc cgccatcgtt ggcgtccgcg aactgagcga agcgtttgcc
     4201 gcactccaag gaacctttca aacactggag aaaacctggg ctcatctgcc aatcctcggg
     4261 aaaaccgagc tgatggatgc ggttccgttg actctcggca cgcagttttc ctcgtttgcc
     4321 gaggcattcg cccgcgaccg ctggcgcacc tttaagtgcg aagagcggct ccgtacggtt
     4381 aatctcggcg gaacggcgat cggcaccggc atcaccgcgc cgcgcagcta tatcttcctc
     4441 gtcaccgaaa aactgcgcga agtcaccggg ctcggcctct gcccagccga aaacagggtt
     4501 gaggccacgg caaacaccga ctgtttcgtc gaggtctccg gcattctgaa tgccgccgcc
     4561 gttaatctga ttaaagtcag cgacgacctg cgccgccttg cgatgttagg tgaaatccga
     4621 ctgcctgccg tgcaggccgg ctcgtcgatt atgcccggta aggtcaaccc ggttattctg
     4681 gaatcagtca tgcagatcgg catcaaagtc aaagcgaaca gcaatatcgt cgccgactgc
     4741 tcagcacgcg gctcgctgca aatctgcgag ttcatgccgc tgctggccga cgccctgctc
     4801 gaatctatcg gtctgctgat cgccgccgcg cagatgctgg cgaaacacgc agccggaatt
     4861 gacgcggatc aaggtaaatg tctggaacgc ctctatgctt ctccggaaat tatcacagcg
     4921 tttgttccgc tgctgggtta cgaccgctgt tctgagctgt tcaaacagtt cgaacagcac
     4981 ggcaaaggaa acatccgcgg gtttttagag aaggaactcg gaaaagagac ggttgaaaaa
     5041 acgctctcgc ctcaaaattt gatatcactg ggccataaat gaaaaccgca ccgaaaagtt
     5101 tacgattgca cattgcgctg ttcggacgca cgaacgtggg caagtccagc tttctgaacc
     5161 tgattgcagg ccaggacgtc tcgattgttt cggaacagcc cggcacaacc accgatgtcg
     5221 tcgaaaaaac gatggaactg cttcccatcg ggccggtggt ttttattgat acagccgggc
     5281 tcgacgacgt taccgcgctg ggcggcaggc gtatcgaaaa aacagaaaaa gtattcgacc
     5341 gcgccgatgt gattcttttg atttgcgaag gaagccggtt cggcgaattt gaaaaagccg
     5401 tggaaacacg cgcggtacaa aagaatgtgc cggtcatccg gattctcaac aaatcagacc
     5461 tatacgaagt atcggattta tcagacctat gcgtgaattc ggaagacctc tcttcccgcg
     5521 aaaaggttct ctcgcaactc aaagcccgcc tgatttccgt ttgcccggaa gaatttattc
     5581 aacccccgcc gctggtcggc gatctggtga agcccggcgg tatcgcggtg ctgattgtgc
     5641 cgattgattt gcaggcgccg aaaggccgcc tgattctgcc gcaggtttca accatccgcg
     5701 atgcgctcga taacgacgcc gccgcactgg tcgtcaagga acgcgaatat gcacatatgc
     5761 tcggccggct gaaaacgccg ccggatctcg tcgtctgcga ctcacaggtg gtgctgaaaa
     5821 tgatcggcga cacgccgaac ggcatcccct gcacaacctt ctcgattctc tttgctcggc
     5881 tgaaaggcga cctgccgaag atggccgccg gcgcggcggc gattgaccgg ctcaaagacg
     5941 gcgacaaggt tctgatcgcc gaatcgtgca gccaccacgc ggcggaagat gatatcggcc
     6001 gggttaaaat tccgcgctgg ctccgccagt actgcggatg cgagctgcaa attgacatct
     6061 acgccgggcg cgattttccc gacaatctca gcgaatacag cctcgttgtg cagtgcggcg
     6121 gctgtatgca caaccggcgc gaaatcctgt cgcgcattga aaaatgcgaa gcggccggcg
     6181 tgccgatcac caactacgga ctttgtattt cccagacaca gggcgtgctc aagcgtgtgc
     6241 tttcgccctt ccccgccgca ctggatgctt ttgaaaggaa cctgaaatga aaatcgatac
     6301 cgaagggctg aagtccttca ttcccgaagc cgaaattttc cagtggctgg aaaaaacgaa
     6361 aaatccgtcc gcagagcggg tgcgtgaaat tatcgccaag tcgctcaata aaaaccggct
     6421 cgatcccgaa gagatggcgg tactgattaa cgccgaagat ccggcgctgg cggatgaaat
     6481 ttttgagggc gcacgtgagc tgaaaaaacg catctacggc aaccgcattg tactgtttgc
     6541 cccgctttac gtaggcaacg agtgcatcaa taactgccag tactgcgggt tccgctgcac
     6601 gaacaaagaa gtttcacgca agacgctgac gatgccggaa ctcgactgcg aaatcgaagc
     6661 gctggtgacg cgcggacata aacgtctgat tctggtttac ggcgaacatc cgcagtacga
     6721 cgcgcagttt attcacgaca ccgtcaaaga agtttatgcc cggaaacacg gcaacggcga
     6781 aatccgccga gtcaacatca acgccgcgcc gctggacgtc gaaggctaca gactggtgaa
     6841 gtccgccggc atcggcacct accagatttt ccaggaaacc tatcatcagg aaacctacaa
     6901 acagatgcat ccgtcgggcc cgaaaagcag ttatctctgg cggctctacg gccttgatcg
     6961 cgcgcaggaa gcaggtatcg acgacgtcgg tatcggcgcg ctgatgggtc tgtttgactg
     7021 gcgttttgaa acgatggccc tgctttacca cacgatccat ctggaagaaa aatttcacgt
     7081 cggaccgcac accatcagct tcccgcgcat tgaaccggcc atcggcacag caatggccga
     7141 tcatccgccg tccggcgtca gcgattacga cttcaagcgt ctcgtcgcca tcctgcgcct
     7201 gtcggtgccg tacaccggtc tgattctgac ctgccgcgaa tcggttgcca tccgcaacga
     7261 aatcatccag ttcggagtgt cgcagatcga cgccggttcc gacattggcg tcggagcgta
     7321 ctcgcagaac aaggaaagct acaaaaagag ccagttcgtt ctcaacgacg gccgctcgct
     7381 cgatcaggtt attcgcgaac tgtgcgaagc cgacttcatt ccaagcttct gtaccggatg
     7441 ctatcgcctc ggccgcaccg gcgaacattt catggaattc gccatccccg gtttcgtgaa
     7501 acggttctgc acgcccaacg ccgtactgac cttcctcgaa tacatggaag actactcgtc
     7561 gccggaaacc aaaaaggccg gcatgaaaca gattgaacgc gaactggaac gaatgcccga
     7621 cggcgaacag aaaacccaac tgctcgaacg catggcgcaa atcaaacagg gtaatcgcga
     7681 cctttatttc tgatccgact gatcggaccc atatgaccta tgccatatta gaaaaagttg
     7741 accgcgacga agagctgacg cctgcggaaa tgaaagcgct gctggaagtt gccgatccgg
     7801 cagaacttca ggctctgtac gactgcgcat accgcgtaaa agcaaagtac gtcggcaagg
     7861 tcgcctattt ccgcggcatc attgagtgca gcaacatttg catcaaggac tgctattact
     7921 gcggcatccg caagagcaac acaaacgtga agcgcttcca gatggatgaa gaggaaatgg
     7981 tgcgcgaagc agtctgggcc tttgaaaacg aatacggctc ggtcgtgatc cagtccggcg
     8041 agcggcagga cgccgcctat attgagctga ttgaacgggt actccggcgg attaaaacgg
     8101 atacggacgg caaactcggc gtcacgctgt cgctcggcga acagacggaa gaaacctacc
     8161 gccgctggcg tgaagcgggc gcgcaccgct acctgctccg catcgaaacc accaatccgg
     8221 cgctgtaccg caaactgcat cccgccgatc acgactttga agtgcgcaaa aactgtctct
     8281 cgctattgaa gaaaaccgga tttcaggtcg gcaccggcgt gatgatggga ctgcccggcc
     8341 agacgatgga cgatctggtc aacgacatcc tgttcatgaa gga
//
LOCUS       DAOPZO010000085         8148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_10549_length_8148_cov_9.171383, whole genome shotgun sequence.
ACCESSION   DAOPZO010000085 DAOPZO010000000
VERSION     DAOPZO010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 8148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8148
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10549_length_8148_cov_9.171383"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..660
                     /locus_tag="PKI68_06510"
     CDS             37..660
                     /locus_tag="PKI68_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53379.1"
                     /translation="MAEELQPLLEQIRKEGVEKAEAQAAEILAQARTQAAQAVREAEA
                     KAKELVAKAQADAEVYTRRSTATLEQAARDLLITVGQGIENMISDIIAESVDSALKVE
                     VLEQMMVKMAQSCAERQGETRIELLIGEQDQRDLVKFFAQKYREKMIHGIELHVDNEI
                     LKGFRVSFADDHVYLDFTRDAIAGALTAFLRPKLAAIVSRVANESAN"
     gene            665..1192
                     /locus_tag="PKI68_06515"
     CDS             665..1192
                     /locus_tag="PKI68_06515"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HNX53380.1"
                     /translation="MNYWYLVASLPYLRFGEKPALSRSAFLSSCAGQLPAAEFQVLSA
                     VFENRCAPDSAAAAQWWSGEVGLRNAVVRVRAKNRGVDAARFLQPHDGFSMAVEEMVT
                     DAFARANPLEQEADLDRIRWGLADELALTAPFGFPGVLAFAVKLRIAERWAGFDGEEG
                     RKIVEELILASAESR"
     gene            1220..2947
                     /locus_tag="PKI68_06520"
     CDS             1220..2947
                     /locus_tag="PKI68_06520"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNX53381.1"
                     /translation="MNLGKIVGVNGNLLTVEFEKPVIQNETGFARLGDVHLKAEVIRI
                     RGCHAELQVFEATTGLKVGDQVEFTGALLSVELGPGLLTQVFDGLQNPLPALAEECGF
                     FLQRGKYLQALPRDTKWKFTPSVKVGDPVQAGSAVGTVPEGIFDHRIMVPFRIAGRLT
                     VKEVSPAGDYTVEEVVAKVADEKGREYEVKLMQLWPVKVPIKCYAERLRPTEPLITKV
                     RIVDTFFPVARGGTYCIPGPFGAGKTVLQQITSRHADVDVVIIAACGERAGEVVETLR
                     EFPELTDPRTGKSLMERTLIICNTSSMPVAAREASVYTAVTIAEYYRQMGLNVLLLAD
                     STSRWAQALREMSGRLEEIPGEEAFPAYLESVIAAFYERGGVVRLHDGSTGSVTIGGT
                     VSPAGGNFEEPVTQSTLKVVGAFHGLSRARSDARRYPAIDPLDSWSKYPSVVDADELK
                     QARRFLREGSEVNQMMKVVGEEGTSIEDFIVYLKGEYLDAVYLQQNAFSDVDAATPAE
                     RQQYVFGALAKILRTKLKFENKEAARSFFQTLTQATRDWNNAAFESEEFKQQQERINA
                     LLAEVTDYA"
     gene            2940..>4180
                     /locus_tag="PKI68_06525"
     CDS             2940..>4180
                     /locus_tag="PKI68_06525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF002555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNX53382.1"
                     /translation="MHKVYHRIEQIAGNVIVVSAEEIRYGELAQVTSDMGVSLAQVIR
                     LEGGRVYLQVFAGGRGISTDSQVRFLGRNMQVPFSQNLLGRVFTGSGVPRDRGPVLSE
                     NMIEIGGPSVNPARRIIPRNMVRTGIPMIDVFNTLVESQKLPVFSVAGEPYNELLARI
                     ALQAEVDVIILGGMGLKHDDYLYFRDYLEENGALSRSVLFVHTAADPTVECLLVPDIA
                     LAVAEQFALADQRVLVLLTDMTNFADAMKEVAITMEQIPSNRGYPGDLYSQLAARYEK
                     AVDFEGAGSITILAVTTMPGDDVTHPVPDNTGYITEGQFYLKHGRIEPFGSLSRLKQQ
                     VNGKTRADHRTIMDTMIQLYAAYKETLEKQSMGFRMSGWDSKLLKYGERFEKEMMALS
                     VNIPLEGALDLGWQILADCFEP"
     assembly_gap    4181..4280
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4281..4865
                     /locus_tag="PKI68_06530"
     CDS             <4281..4865
                     /locus_tag="PKI68_06530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF002565.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNX53383.1"
                     /translation="KNELKAQRDALTRFRRYLPTLLLKKQQLQLEMRGLEAQIVAKRQ
                     EEDKARADLASWVKLFSEPFDFTPYVQIAEIRRSSGNIAGVEIPLFESLRFEEALPDL
                     FATPAWVDSAMDLLRQLIRLRVERQIIEEQHRLLSDELRTTSQRVNLFEKVKIPECRE
                     NIRVIRIFMGDQQTAAVARSKIAKGKSVEKVRSA"
     gene            4874..6628
                     /locus_tag="PKI68_06535"
     CDS             4874..6628
                     /locus_tag="PKI68_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53384.1"
                     /translation="MKKLTLLCTRAMQEQTLEALRELGVVHLQPVQLPEGSGLDEARD
                     LFAHLQRALEVLPKHPHALPSGKTAQETVEAVWSLIHRKQALTEQIETLDMDVRRYEP
                     FGSFSPAAVRELEAKGISVKLYKAPAKVRPEIPDGASMVELSRTRSTVCFALIGRAGI
                     TLDAEEIALPAQSLAELRKQLETLRAELVQTEAEFHSYAGDHAVVAALAEEATGRVHY
                     LEARAGMGSAEEIVYLRGFFPGEQEAAVRKTAAACGWAAMVYDIAPEDNPPTLLRNPK
                     WVSPIQAVFKIIGVTPGYHEVDISRFFLLFFSLFFAMLVGDAGYGLLFLGLTLFAKRR
                     FRKLPQQGFNLLMITSTCTIIWGTLTGVWFGVANLPAVLSQFKIGWLTGERANENIML
                     LCFFIGAVHLTIAHAWNTLRIINSWQALAQLGWIGSTWTMYFTARTMVLYYPFPPVML
                     WIMAVSVVLIVLFMNPVHKVKSEWFNYAVLPLSIVSNFTDVVSYLRLFAVGTAGFAVA
                     GAFNGMAAEAGAGGFVGSLGAALILFFGHTLNILLSTMGVMVHGIRLNTLEFSGHIGM
                     QWTGQHYRPFRKFSNKEL"
     gene            6645..7094
                     /locus_tag="PKI68_06540"
     CDS             6645..7094
                     /locus_tag="PKI68_06540"
                     /inference="COORDINATES: protein motif:HMM:NF005174.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53385.1"
                     /translation="MDAALMEGLGRAGGAMALGFAAIGSSLGAGEAGMAAIGAWKKCY
                     AQNRAAPFILLVFIGAPLSQTIYGMILMNVMKNSASFENWPAMISAGLVGGIALGVSA
                     WYQGKAGASGADAMGETGQGFGNYLTTIGVIETVALFVMVFAQGALK"
     gene            7166..>8148
                     /gene="pheS"
                     /locus_tag="PKI68_06545"
     CDS             7166..>8148
                     /gene="pheS"
                     /locus_tag="PKI68_06545"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNX53386.1"
                     /translation="MNLQELNQIRETALAEVRGAADAAALEALRIKYLGRNGLLPEVM
                     AQLKEVPNEEKPLFGKGANALKNELTELIKERQTLLASGAKKNDAFDPTLPGLWPSLG
                     SEHPISKVTARIVEIFQSIGFTVADGPDIETVWNNFDALNTPADHPSRDEQDTFYLNS
                     GELLRCHTSPVQVRYMESHQPPIRIIAPGRCYRRDTPDATHSANFHQVEGLFVAEKVS
                     MADLKGTLAYFAKQFLGDEVKIRFRPHFFPFTEPSFEVDFFFEVGGKARWIEILGAGM
                     VDPNVFRKVGYDPDKVTGFAFGMGIERLAMIMYGIKDIRNLYENDVRFLSQ"
BASE COUNT         1629 a         2221 c         2554 g         1644 t
ORIGIN      
        1 ccggcaagct tttagcaacc cggactggag tgaattatgg ccgaagaatt gcaaccgctg
       61 ctcgaacaga ttcgcaagga aggcgtcgaa aaggccgagg cgcaggccgc cgaaattctt
      121 gcgcaggcca gaacccaggc ggcccaggct gtccgcgagg cggaggccaa agccaaagag
      181 cttgttgcca aagcgcaggc cgatgccgag gtgtataccc ggcgcagcac cgcgacactc
      241 gaacaggccg cgcgcgatct gctgattacc gtcgggcagg gcatcgaaaa catgatttcc
      301 gacattattg cggaatcggt tgattcggcg ctgaaggtcg aggtgctgga acagatgatg
      361 gtgaaaatgg ctcagtcctg cgccgagcgt cagggtgaaa cccgcatcga gctgctgatc
      421 ggggaacagg atcagcggga tctggttaaa ttcttcgccc agaaataccg cgagaaaatg
      481 atccatggca tcgagctgca cgtggataac gaaatcctga aaggcttccg ggtcagcttt
      541 gccgacgatc atgtctatct cgattttacc cgcgacgcga ttgccggcgc gctgacggct
      601 ttcctgcggc ccaagctggc ggcgattgtc agccgcgtgg ccaacgaatc ggcgaactga
      661 ccgcatgaac tactggtatc tagttgcctc cctgccgtat ctgcgtttcg gcgaaaagcc
      721 ggcgctgagt cgcagtgcat ttctttcctc ttgtgccggg cagttgccgg cggcggagtt
      781 ccaggtgctg agcgcggtat tcgaaaaccg ctgtgcgccg gacagtgctg ccgctgcgca
      841 gtggtggagc ggcgaggtcg ggttgcgcaa tgccgtcgtg cgggtgcgcg caaaaaaccg
      901 cggggtcgat gcggcacgtt ttcttcagcc tcacgacggg ttcagtatgg cggttgaaga
      961 gatggtaacg gatgcctttg cgcgcgccaa tccgctggag caggaggcgg atctggatcg
     1021 gatccgctgg gggctggccg atgagctggc gctgacggct ccgttcggct ttcccggtgt
     1081 tctggcgttt gccgtcaagt tgcgcatcgc agagcgctgg gccggtttcg atggcgaaga
     1141 gggacgtaag atcgttgagg agttgattct ggcgtccgcc gaatcccgat aaccataact
     1201 gataacggat aattgcatta tgaaccttgg aaaaattgtc ggtgtaaacg ggaacctgct
     1261 gacggtggag tttgaaaagc cggtgattca gaacgaaacc gggtttgccc gcctgggcga
     1321 cgtccatctg aaggcggagg tgattcggat ccgcggatgc cacgccgagt tgcaggtgtt
     1381 tgaagcgacg accggcctta aagtcggcga ccaggtggag tttaccggcg cgctgctgtc
     1441 ggttgagctc ggcccggggc tgctgacgca ggtgtttgac ggtttgcaga atccgctgcc
     1501 ggcgctggcc gaagagtgcg gcttttttct tcagcgcggc aaatatctgc aggcgctgcc
     1561 gcgcgacacg aaatggaagt ttacgccgtc ggtgaaagtg ggcgacccgg tgcaggccgg
     1621 ctccgccgtc ggaaccgtgc cggaaggaat ttttgatcac cggatcatgg tgccgtttcg
     1681 catcgccggg cgtttgaccg ttaaagaagt ctctccggcc ggcgactaca cggttgaaga
     1741 ggtggtggca aaagtcgccg acgaaaaagg gcgcgagtac gaggtgaagc tgatgcagct
     1801 ctggccggtc aaagtgccga tcaagtgcta tgccgaacgg ctgcgcccga ccgaaccgct
     1861 gatcaccaaa gtgcgcattg tggacacctt ttttccggtc gcgcgcggcg gaacctattg
     1921 cattcccggc ccgttcggcg ccggcaaaac cgttttgcag cagatcacca gccgccacgc
     1981 ggatgtcgat gttgtgatta tcgcggcctg cggcgagcgc gccggtgaag tggttgaaac
     2041 gctgcgcgag tttccggagc tgaccgatcc gcggaccggc aagagtttga tggagcgcac
     2101 gctgattatc tgtaacacca gttcgatgcc ggtggccgcg cgcgaagcgt cggtttacac
     2161 cgccgtgacg attgcagaat attaccgcca gatggggctg aacgtgctgc tgctggccga
     2221 ctccacctcg cgctgggcgc aggcgctgcg cgaaatgtcg ggccgcctcg aagagattcc
     2281 cggcgaagaa gcattccccg cgtatctgga atccgtaatc gccgccttct acgaacgcgg
     2341 cggcgtggtg cggctgcacg acggctcgac cggttcggtg acgatcggcg ggacggtttc
     2401 gccggcgggc ggtaattttg aggagccggt gacgcagtcc acgctcaagg tggtcggcgc
     2461 gtttcacggg ctgtcgcgtg cgcgttcgga tgcgcgccgc tatccggcca tcgacccgct
     2521 cgacagctgg agcaagtacc cgagcgtggt ggacgctgac gagctgaagc aggcgcgccg
     2581 cttcctgcgc gaaggcagcg aagtcaatca gatgatgaag gtcgtcggcg aagaaggcac
     2641 ctcgattgag gacttcatcg tttatctgaa aggtgaatac ctcgacgcgg tctatctgca
     2701 gcagaacgcg ttcagcgatg tggatgccgc gacgccggcc gaacggcagc agtatgtgtt
     2761 cggcgcgctg gcgaaaatcc tgcgcacgaa actgaaattt gaaaacaaag aagccgcccg
     2821 cagtttcttt cagacgctga cgcaggcgac gcgcgactgg aataatgccg cgtttgagag
     2881 cgaagaattt aagcagcagc aggagcggat taacgcgctg ctcgcggagg tcacggatta
     2941 tgcataaggt ttatcaccgc atcgaacaga tcgccggcaa cgtcatcgtg gtcagcgccg
     3001 aagagatccg ctacggcgag ctggcgcagg tgacgtccga tatgggcgtg tcgctggcgc
     3061 aggtgatccg gctggaaggc ggacgcgtct atctgcaggt cttcgccggc ggacgcggca
     3121 tttcgaccga ctcgcaggtg cgcttcctcg gccgcaacat gcaggttccg ttttcgcaaa
     3181 acctgctggg gcgtgtgttt accggcagcg gcgtgccgcg cgaccgcggg ccggtgctta
     3241 gcgaaaacat gatcgagatc ggcgggccgt cggtgaatcc ggccaggcgc attattccgc
     3301 gcaacatggt ccgcaccggg attcccatga tcgacgtgtt taacacgctg gtcgagtcgc
     3361 agaagctgcc ggtcttttcg gtggcgggcg agccctacaa cgaactgctg gcgcgtatcg
     3421 ccctgcaggc cgaggtcgat gtgatcattc tcggcggcat ggggctcaag cacgacgact
     3481 atctgtattt ccgcgactat ctcgaagaaa acggcgcgct gtcgcgctcc gttctctttg
     3541 tccacacggc cgccgatccg acggtggaat gcctgctggt tccggatatc gcgctggccg
     3601 tcgccgaaca gttcgcgctg gccgaccagc gcgtgctggt gctgctgacc gatatgacca
     3661 acttcgccga cgcgatgaag gaagtcgcca ttacgatgga acagattccg tccaaccgcg
     3721 gttatcccgg cgacctctac agccagctgg ccgcgcgcta cgaaaaggcc gtggactttg
     3781 aaggcgccgg ttcgatcacg attctggccg tgacgacgat gccgggcgac gacgtgaccc
     3841 atccggtgcc ggacaacacc ggctacatca ccgaagggca gttttatctc aagcacggac
     3901 gcatcgagcc gttcggttcg ctcagccgcc tgaaacagca ggtcaacggc aagacgcgcg
     3961 ccgaccaccg caccatcatg gacacgatga ttcagctcta cgccgcctat aaggaaacgc
     4021 tggagaagca gtcgatgggc ttccgcatga gcggctggga cagcaagctg ctgaaatacg
     4081 gcgagcggtt tgagaaagaa atgatggcgc tctccgtgaa cattccgctc gaaggcgcgc
     4141 tcgacctcgg ctggcagatt ctggccgact gttttgagcc nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn aaaaacgagt tgaaggcgca gcgcgacgcg ctgacgcgct
     4321 tccggcgcta tctgccgacg ctgctgctca agaagcagca gttgcagctc gaaatgcgcg
     4381 ggctcgaagc gcagatcgtc gccaagcgcc aggaggagga caaagcgcgc gccgatctgg
     4441 cctcctgggt gaagctgttt tccgagccgt tcgattttac gccctatgtg cagattgcgg
     4501 aaatccgccg gtccagcggc aatatcgccg gggtcgagat cccgctgttt gaaagcctgc
     4561 gctttgaaga agcgctgccc gatctgttcg ctacgccggc ctgggtggac agcgccatgg
     4621 atctgctgcg tcagctcatc cggctgcgcg tggagcggca gatcatcgaa gagcagcacc
     4681 gcctgctgtc cgacgaactg cgtacgacgt cgcagcgcgt gaatctgttc gagaaggtga
     4741 agattccgga atgccgtgaa aatatccgcg tcattcgcat tttcatgggc gaccagcaga
     4801 cggcggccgt cgcccgctcc aagatcgcca aaggcaagag tgtagaaaag gtgagatcgg
     4861 catgatcgtc aaaatgaaaa aactgacgct gctgtgcacc cgcgcgatgc aggagcagac
     4921 gctggaggcc ctgcgggaac tcggcgtggt gcatttgcag cctgttcagc ttccggaagg
     4981 ctccggtctg gatgaagcgc gcgatctctt tgcacatctt cagcgggcgc tggaagtgct
     5041 gccgaaacat ccgcatgctt tgccgtccgg aaaaacggcg caagaaacgg tcgaagcggt
     5101 ttggagtctg attcaccgta aacaggcgct gactgaacag attgaaacgc tcgatatgga
     5161 cgtccgccgt tacgaaccgt tcggcagttt ttctccggca gctgttcgtg agctggaggc
     5221 taaaggcatc agcgttaaat tatacaaagc acctgcaaaa gtgcggccgg agattcctga
     5281 cggcgcgtca atggttgagt tgagccgcac acgcagcacg gtctgttttg cgctgattgg
     5341 tcgcgcagga ataaccttgg atgccgaaga gattgcgttg cccgcgcagt cactggcgga
     5401 actgcgcaaa cagctggaaa cccttcgcgc ggagctggtg cagacagaag ctgaattcca
     5461 cagctacgcc ggcgatcacg cggttgttgc cgcgctggcg gaggaggcga ccggccgcgt
     5521 gcattatctc gaagcgcgcg ccgggatggg ttctgccgaa gagattgttt acctgcgcgg
     5581 ctttttcccc ggcgagcagg aagcggctgt ccggaaaacg gcggcggcct gcggctgggc
     5641 ggcgatggtt tatgacattg cacctgaaga taacccgccg accttgctgc gcaatccgaa
     5701 atgggtcagc ccgattcaag cggttttcaa aattatcggc gttacgcccg gttaccacga
     5761 agtggatata agcagattct ttcttttatt ttttagtctg ttcttcgcca tgctggttgg
     5821 cgacgccggc tatggactgt tgtttctcgg gctcacgctg tttgccaagc ggcgtttccg
     5881 taaattgccg cagcagggat ttaatctgct gatgattacc agcacctgta cgatcatctg
     5941 gggcacgctg accggcgtct ggttcggggt ggcgaacctg cctgcggtac tcagtcagtt
     6001 taaaatcggc tggctgaccg gggagcgggc gaatgagaac atcatgctgc tctgtttctt
     6061 tatcggagcg gtgcatctga cgattgcgca tgcctggaac acattacgga ttatcaacag
     6121 ctggcaggcg ctggctcagc tcggctggat cggcagcacc tggacgatgt atttcaccgc
     6181 gcggaccatg gtgctttatt atccttttcc gccggtcatg ctctggatca tggcggtcag
     6241 tgtggtgctg atcgttctgt tcatgaatcc ggtgcataag gtgaagagcg agtggtttaa
     6301 ctatgcagtt ctgccgctca gtatcgtcag taattttaca gacgtggttt cttatctccg
     6361 gttgttcgct gtcggaaccg ccgggtttgc cgtggccggc gcgttcaacg gcatggcggc
     6421 tgaggctggc gcaggcgggt tcgtgggcag tctgggcgcg gcattgattc ttttcttcgg
     6481 ccatacgctc aatattctgc tttccacgat gggcgtgatg gtacacggca tccggttgaa
     6541 cacgctggaa ttttccgggc atatcggtat gcagtggacc gggcagcatt accgcccgtt
     6601 ccggaagttt tcaaataaag aattataatc aaggaggact cagtatggat gcagcattga
     6661 tggaaggatt gggacgggcg ggcggcgcaa tggctcttgg tttcgcggca atcggctctt
     6721 cgcttggcgc gggcgaggcg ggtatggcgg cgatcggagc ctggaaaaag tgctatgcac
     6781 agaaccgggc ggctccgttc attctgctgg ttttcattgg cgcgccgctg tcgcagacca
     6841 tttacggcat gattctaatg aatgtcatga aaaactcggc gtcgtttgaa aactggccgg
     6901 cgatgatcag tgcagggttg gtcggtggta tcgcgctggg tgtttcagcg tggtatcagg
     6961 gaaaggccgg agcttcaggc gcggatgcaa tgggcgaaac cgggcagggc ttcgggaatt
     7021 acctgactac tatcggagtt attgaaaccg tggcactgtt tgtgatggtt tttgcacaag
     7081 gtgcgctcaa ataaagtacc ggaatttttt tccagacatt ggaacggttt atcagtagtt
     7141 tctctgaata ttttcgggaa aaactatgaa tttgcaggaa ctcaatcaga tcagagaaac
     7201 ggcactggcg gaagtccgcg gcgcggcgga tgccgccgcc ctcgaagcgc tgcgcatcaa
     7261 atatctgggc cgcaacggac tgctgcccga agtcatggcg cagctcaaag aggtgccgaa
     7321 cgaagaaaaa ccgctgttcg gcaaaggcgc caacgcgctc aagaacgagc tgacggagct
     7381 gattaaagaa cggcagaccc tgctggcttc cggcgccaag aaaaacgatg cgttcgatcc
     7441 gaccctgccg ggcctctggc catcgctcgg ttccgagcat ccgatcagca aagtgaccgc
     7501 ccgcatcgtc gaaatctttc agtcgatcgg cttcacggtc gctgacgggc cggacatcga
     7561 aacggtctgg aacaacttcg acgcgctcaa cacgcccgcc gaccatccgt cgcgcgacga
     7621 gcaggacacg ttttatctca acagcggcga gcttctgcgc tgccatacgt cgccggtgca
     7681 ggtgcggtac atggaaagcc atcagccgcc gatccgcatc atcgcgccgg gccgctgcta
     7741 ccgccgcgat acgcccgacg ccacgcacag cgccaacttc catcaggtcg aagggctctt
     7801 cgtcgcggaa aaagtttcca tggccgacct caaaggcacg ctggcctatt tcgccaaaca
     7861 gttcctcggc gacgaggtga aaatccgttt ccgcccgcac tttttcccct tcaccgagcc
     7921 gagctttgag gtcgatttct ttttcgaagt cggcggcaag gcgcgctgga ttgaaatcct
     7981 cggggcgggc atggtcgatc cgaacgtctt ccgcaaggtc ggctacgatc cggataaggt
     8041 caccggcttc gccttcggca tgggcatcga gcggctggcg atgatcatgt acggcatcaa
     8101 agacatccgc aacctttacg aaaacgacgt gcgcttcctg tcgcagtt
//
LOCUS       DAOPZO010000086         8081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_10665_length_8081_cov_10.910416, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000086 DAOPZO010000000
VERSION     DAOPZO010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10665_length_8081_cov_10.910416"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PKI68_06550"
     CDS             complement(<1..713)
                     /locus_tag="PKI68_06550"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNX53387.1"
                     /translation="MTHPARIHALNRKPVRATGGFVLYWMQQSQRAEWNHALEYAITR
                     ANALGLPVAVVFGLMDDYPEATERHYAFMLEGLAETFQTLEQRGIGAFIGRGHPADVA
                     LKAGRNAALMVCDRGYLRHQAEWRARVADEADCEVVEVESDVVVPVETASDHAEYMAR
                     TLRPKLLKRLDEFLQPVKKINPRICTNRHEFFQGLEKTVPKVPNLGKFVGGTSEAKKR
                     LKKFIAESLAVYEAHSNQP"
     gene            complement(710..1216)
                     /locus_tag="PKI68_06555"
     CDS             complement(710..1216)
                     /locus_tag="PKI68_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNX53388.1"
                     /translation="MGSRGIQLVGMDVKKLLTLLNKALADEWLAHYQYWVGSKVIAGP
                     MKDAVIAELVLHAAEEMAHAELLTTRIIQLGGTPVLTPADWFKLTNCKYEAPADPFVQ
                     KVLEQNIAGEQCAIGVYEKLMKVTKDADPVTYNIALQIITQEIEHEEDLQNLLADLQM
                     MFNHFGKK"
     gene            complement(1245..1538)
                     /locus_tag="PKI68_06560"
     CDS             complement(1245..1538)
                     /locus_tag="PKI68_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53389.1"
                     /translation="MQKKDWIAVGAKLLGIYIAVMALIGAGSAVLNTLMTFIFSDRPA
                     SVGFFKMLWIIVVKVLLFNLVVPAAQGFVAWLLLKRTDWCLRKAGLGCEPPQM"
     gene            complement(1649..2548)
                     /locus_tag="PKI68_06565"
     CDS             complement(1649..2548)
                     /locus_tag="PKI68_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX53390.1"
                     /translation="MWIYLGLISCVLLGLYDVCKKHALEGNAVLPVLFLSTVAGLIPM
                     LFCLAASRLAPDFTQQIHCYVPVATRSEHLHIFLKAFIVSVSWVLAYFAMKHLPISIF
                     SPIRASGPVWTLIGALLIFHERPLPLHWAGIAVILCAYYGFSVLGRKEGIIFLRNRWV
                     FCIALATFIGTLSTLYDKYLLQQLGYATMLVQFWFAFYNVVLLGLFTLCCWLPRRRTF
                     TPFRWRGSILLIGLLLIASDFVYFNAVKQPDALILILSMLRRSSVVVSFFAGGFIFGD
                     VNLRGKAWALAGIMAGILLIILA"
     gene            2697..3053
                     /locus_tag="PKI68_06570"
     CDS             2697..3053
                     /locus_tag="PKI68_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNX53391.1"
                     /translation="MTVPGIDIDVLLEATGNDRTIAAELMRLFFELTGQEQARLADAV
                     AQGNHAAASAVAHKVAGSCVACGMSGIYARFKELENLCKEAMPEDARERIQAIDRELQ
                     DARRNLEKYFNCTLVS"
     gene            3050..3820
                     /locus_tag="PKI68_06575"
     CDS             3050..3820
                     /locus_tag="PKI68_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX53392.1"
                     /translation="MKSILFIDDDRFVTTLYKAKLQNEGFAVDVAHSANEAFEQLEHK
                     KHPDIIVLDLNMPGINGVIILKTIRSVPQLQHIPVIIFSSGYVKALVDEASQLGIYKF
                     FAKAQCPPKMLISAITEILSKMAIPASALTADADTASGSLTADDLPALLYMFVASEDP
                     DALRSTLLKIYKASRDCINKALAADESTAQGKLSRMVEKLFEDLYAHPEHITASTKHT
                     LAAGLQKLSDIEAENAGPILDSELALKDILRTLETQSA"
     gene            complement(3808..5580)
                     /locus_tag="PKI68_06580"
     CDS             complement(3808..5580)
                     /locus_tag="PKI68_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX53393.1"
                     /translation="MALLSLQNLHIAFGGPPLLDGVTLQIEPGERICLLGRNGEGKST
                     LLKIVSGDLEPDEGEIVRSRDVRIARLQQTVPQDVHGTVFDLVSSDWNHEHALDHPVE
                     KAISLLGLDPEQEFSSLSGGMRRRALLAKALVNEPDLLILDEPTNHLDIESIQWLENF
                     LLRWRGTVLFVTHDRVFLRKLATRIVELDRGRLTSWACNYETYLARRQALLDAEEGQW
                     AEFDRKLAQEEVWIRKGIKARRTRNEGRVRELEKMRSERSQRRDRVGTVKLQLNEAER
                     SGRKVITAKDVFSGYDGVDIIKNFSVEILRGDRIGIIGPNGCGKSTLLNTLLGQLAPR
                     QGEIKHGTRLEIAYFDQHRVALDEQKSVKWNLCMDNDYVQTGTGRQHAIGYLQNFLFS
                     PADARQPVSSLSGGERNRLMLAKIFAQSSNVLVLDEPTNDLDVETLDLLEELLVEYQG
                     TVLLVSHDRAFINNVVTETLVFEGDGAINEYVGGYDDWLTQRAAARDQKSDVKEPQIS
                     AGKVARKRKLSNREREDLKNLPRKIEKLEAELAELNRKLNDPDFYQQPADEIKTVTDR
                     AAAIPVEMDAAFARWAELDGSQAD"
     gene            complement(5628..7289)
                     /locus_tag="PKI68_06585"
     CDS             complement(5628..7289)
                     /locus_tag="PKI68_06585"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HNX53394.1"
                     /translation="MKKKILFIQLPLLDNDTSTPNENFPFAALWLSHALSQHPEGEFW
                     DVVPAPAKWDELDNARLFEEVLASEVEAVAFTLCLWNVERSLRLASRLREARPDLFLI
                     AGGPEIAEDHPFLLEDPVFNALVTGEGEAVFPEILKGIRTGKLPAYDNVLISSDKGWR
                     RGSKPPPQVDLASVQVPASALMDCIKARPAVYIETSRGCPLTCTYCRYHHLQKGVRAL
                     PAEAAAERVRQLRAAGAREIRFVDPTFNARGDFVRLLERLAEINADRKLGFFAELRAD
                     TLTPEQAALMAKVNFIDVEVGVQSTDPKVLRTIRRPANIAQVERGIRLLTGAGVRVTL
                     DLMYGLPEQTFDDVLRSIEWARGLGPGITVQCMQTLLLPGTDLRGQAEQYGMKAMPLP
                     PYGVFSTSTMPEEDMRRIEAFLHDSDDMPADPITSRFTGYRLTGLFKEQAGLNRRALI
                     FRGGGLHGRRKEICRMIEQVIADEPDVLWQFILCPEREEPIELLEALIATIKKQPLHL
                     LDRFASAELFGQIAARRIFVRLQKGRTYDAVWRDQVEELLGRHFV"
     gene            complement(7377..>8081)
                     /locus_tag="PKI68_06590"
     CDS             complement(7377..>8081)
                     /locus_tag="PKI68_06590"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNX53395.1"
                     /translation="AFLAHLDWLAECGVRNLLVNGTTGEFFSLSPAEQLQLLQTAREN
                     WDGIIIAHAGSPALASCGEAAHVAEEIGADFLVSLPPFYFAGAPGSGLVRWFNELAES
                     TALPLILYNFPKHTGNPLTPEILKQVPHWGLKDSSADLALIPATPRYFVGGDRKISEA
                     YAAGAKGFVSASANIDPLPYLRIEQEQSPENQAAVDVVNARTNGPFAIARIKQALSEI
                     IPGYPPHVRPPLTTQQ"
BASE COUNT         1784 a         2423 c         2241 g         1633 t
ORIGIN      
        1 tgcggctgat tgctgtgcgc ttcgtaaacc gccaggcttt cagcgatgaa ttttttcaat
       61 cgcttcttcg cctcagacgt tcccccaaca aattttccga ggttcggaac tttgggaacc
      121 gttttttcca aaccctggaa gaattcgtgt cgattcgtgc aaattcgcgg gttgattttt
      181 ttcaccggct gcaggaattc atcgagccgc ttcaagagct tcggacgcag cgtgcgcgcc
      241 atgtattcgg cgtgatccga ggcggtttcg accggcacga cgacatcgct ttcgacttcg
      301 acgacctcgc agtccgcctc gtcggcgacg cgggcgcgcc attcggcctg gtggcgcagg
      361 tagccccggt cgcagaccat cagcgcggcg ttccggcccg ctttcagtgc gacgtccgcc
      421 ggatggccgc gcccgataaa ggctccgatg ccgcgctgtt ccagggtctg aaaggtttcc
      481 gcgagtcctt cgagcatgaa ggcgtagtgc cgctccgtgg cctccggata gtcgtccatc
      541 agaccgaaga cgacggcgac gggcaggccg agcgcgttgg cgcgagtgat tgcgtattcg
      601 agcgcgtggt tccattcggc gcgctggctc tgctgcatcc aatacaaaac aaacccgccg
      661 gttgcccgga cgggtttgcg gttcagtgcg tggatacgcg ccggatgtgt cattttttgc
      721 cgaagtgatt gaacatcatt tgcaggtccg ccagcaggtt ctgcaggtct tcttcgtgtt
      781 cgatttcctg cgtgatgatc tgcaaggcga tgttgtaggt gaccggatcg gcgtccttcg
      841 tcaccttcat cagcttttca tagacgccaa tggcgcactg ttcgccggcg atgttctgtt
      901 cgagcacctt ctgaacgaac ggatctgccg gcgcttcata tttacagttg gtgagcttga
      961 accagtcggc cggcgtcagc accggcgttc cgccgagctg gatgatgcgg gtggtgagca
     1021 gttcggcatg cgccatttct tcggcggcat gtaataccag ctcagcgatt accgcatctt
     1081 tcatcggccc ggcgatgact ttggagccga cccagtactg gtagtgcgcc agccattcgt
     1141 cggcgagcgc tttgttgagt agcgtcagca gcttcttgac gtccattcct acgagttgaa
     1201 ttccgcgact gcccatggaa cacctcctgt tttatgttta ccggttacat ctgcggcggc
     1261 tcgcagccca gtccggcttt ccggaggcac cagtcggtgc gcttgagcag cagccaggcg
     1321 acaaatccct gggcggccgg aacaaccaga ttgaacagca gcactttgac cacaatgatc
     1381 caaagcattt tgaagaatcc gacgctggcc ggacggtcgg agaaaatgaa ggtcatcagc
     1441 gtgttcagca ccgcagaccc cgcaccgatt aacgccatga cggcgatata gatccccaac
     1501 agcttagctc ctactgcaat ccaatctttt ttctgcataa tttgaccgcg cgccgcgggt
     1561 tgtttgccgg cgcgaacttc ctttcgggcg ttagactacc gctttttttt tgaaaagcaa
     1621 ccggcaggaa ggaggtttga ggctgaaatc aggccagtat gatcaggaga atcccggcca
     1681 tgattccggc cagcgcccac gccttgccgc gcaggttgac gtcgccgaag ataaacccgc
     1741 cggcgaaaaa ggagaccacc acgctgctgc ggcgaagcat ggagagaatc agtatcaaag
     1801 catccggctg tttgacggca ttgaaataga caaagtcgga ggcaatcagc agcaggccga
     1861 taagaagaat acttccgcgc caccggaacg gcgtgaaggt tctgcgccgc ggaagccagc
     1921 agcagagggt gaacaggccg agcagaacga cattgtagaa tgcgaaccag aactggacca
     1981 gcatcgtggc atagccgagc tgctggagca gatatttatc gtacagcgta ctgagcgtac
     2041 cgatgaaggt ggcgagagcg atgcagaaaa cccagcggtt gcgaagaaag ataatccctt
     2101 ccttccgtcc aagaaccgag aagccgtaat aggcgcacag tatgacggca atgccggccc
     2161 agtgcagcgg cagcggacgt tcatgaaaaa tgagcagcgc gccgatcagc gtccagaccg
     2221 ggccggaggc gcggatcgga gaaaagatcg aaatcggcag gtgtttcatg gcgaagtagg
     2281 ccagcaccca ggaaacggaa acgatgaagg ctttgaggaa aatatgcaga tgttcggaac
     2341 gcgtcgcaac cggtacatag cagtggatct gctgcgtaaa atccggagcc aggcgcgatg
     2401 ccgccagaca gaaaagcatc gggatgagcc cggccacggt ggacagaaac agcaccggca
     2461 gcacggcatt accttccagc gcgtgctttt tacagacatc gtaaagcccc agcagcacac
     2521 aggaaatcag tccgagatat atccacataa aaacagtccg gcaatgttag acagaagcgg
     2581 ggcatggtac gcattccgct ccggcgcaca agacgaaacc ggaaccgttc agcgccgcgt
     2641 aacttttcat ttctttacca ttgcacttca gcgttaaggt cttatagtcc gcggacatga
     2701 cggtacccgg catagatatt gatgttctgt tggaagccac cggcaacgac cgtacaatcg
     2761 cggccgagct gatgcggctt ttctttgaac tgaccggcca ggaacaggcc cgtctggccg
     2821 acgccgtcgc tcagggaaat catgccgccg cctcggcggt tgcccataaa gtcgccggat
     2881 cctgcgtcgc ctgcggaatg agcggcatat acgcccggtt caaagaactg gaaaatctct
     2941 gtaaagaagc catgccggaa gatgcccgcg agcggattca ggccatcgac cgggagctgc
     3001 aggatgcccg ccggaatctg gaaaagtatt tcaactgtac cctcgtgtca tgaaatcaat
     3061 tctgttcatc gatgatgacc ggttcgtaac cacgctctac aaagccaagt tacagaacga
     3121 gggttttgcg gtggatgttg cgcacagcgc aaacgaagcc ttcgaacagc tcgaacacaa
     3181 gaaacacccg gacattattg ttctcgacct gaacatgccg gggatcaacg gcgtcatcat
     3241 actcaaaacc atccgcagcg ttccgcagct gcagcatatt cccgtcatca ttttttccag
     3301 cggatatgtt aaggcgctgg tggatgaagc ttcacagctc ggcatttaca agttcttcgc
     3361 caaagcacag tgcccgccga aaatgctgat ttcagccatc acagagattc tcagtaaaat
     3421 ggccataccg gcctccgcac tgacagccga tgcggacacc gcgtccggat ccctgaccgc
     3481 cgacgacctg cccgccctgc tgtatatgtt tgtcgcttcg gaagaccccg acgccctgcg
     3541 ctccactctg ctgaaaattt acaaagcctc ccgcgactgc atcaacaaag cactggcggc
     3601 agacgaaagc accgcacagg gcaaactgag ccgcatggtg gagaaactgt ttgaagacct
     3661 ctacgcccac ccggaacaca tcaccgcctc caccaaacac acactggcgg caggacttca
     3721 gaaactcagt gatattgagg ctgaaaatgc cgggccgata ctggattccg aactggcact
     3781 gaaagacatt ctgcggacac tggaaactca atccgcctga gaaccgtcca gctccgccca
     3841 gcgggcgaac gcggcatcca tctcgaccgg aatagcagcg gcccggtcgg tgaccgtttt
     3901 aatctcatcc gccggctgct ggtaaaaatc cggatcgttc agcttccggt tcagctccgc
     3961 cagctcagcc tcaagcttct caatcttcct gggcagattc ttgagatcct ctcgctcgcg
     4021 attgctcagc ttgcgcttcc tggccacttt tccggcagaa atctgcggtt ctttgacgtc
     4081 tgacttctga tctctggccg cggcccgctg cgtcagccag tcatcgtagc cgccgacata
     4141 ttcattgatc gctccgtcgc cttcaaacac cagcgtttcc gtcaccacat tattgatgaa
     4201 agcgcggtcg tggctgacca gcagaaccgt tccctgatat tccaccagca gctcttcgag
     4261 cagatcgagc gtttcgacat cgagatcgtt ggtcggctcg tcgagcacca gcacgttgga
     4321 agactgggcg aaaattttgg caagcatcag ccggttacgt tcaccgccgg aaagcgaact
     4381 gaccggctgc cgggcatccg ccggggagaa aagaaaattc tgcagataac cgattgcatg
     4441 ctgccgcccg gtaccggtct gcacataatc attgtccatg cagagattcc acttcacgct
     4501 cttctgctca tccagcgcga cacggtgctg atcgaaatag gcaatttcca gcctggtgcc
     4561 gtgtttgatt tcgccctgcc tcggcgcgag ctgccccagc agggtgttca gcagtgtaga
     4621 ctttccgcat ccgttggggc cgataattcc gatccggtcg ccgcgcagaa tttcgacgga
     4681 gaaattttta atgatatcca cgccgtcata tccggagaaa acatctttgg ccgtaatcac
     4741 tttccgtccg gaacgttccg cttcgttcag ctgcaactta accgtaccga cccggtcgcg
     4801 ccgctggctg cgctcgctgc gcattttttc cagttcacgt acgcggcctt cgttgcgggt
     4861 gcgccgcgct ttgatccctt tacggatcca gacctcttcc tgcgccagct tgcggtcgaa
     4921 ctccgcccac tggccttcct cggcgtcgag cagcgcctga cggcgggcta aataggtttc
     4981 atagttgcag gcccagctgg tcagccgccc gcgatccagt tctacaatac gcgtcgccag
     5041 cttccggaga aaaacgcggt catgcgtcac aaaaagcacc gtgccgcgcc agcgcagcag
     5101 aaagttttcc agccactgga tggattcgat atcgaggtgg ttggtcggct cgtcgagaat
     5161 cagcagatcg ggttcgttaa ccagcgcctt ggccagcagc gcgcggcggc gcatcccgcc
     5221 ggaaagcgaa gagaactcct gttccggatc gagacccagc agagagatcg ccttctccac
     5281 gggatgatcc agcgcgtgct cgtggttcca gtctgaggaa accagatcga aaaccgtccc
     5341 atgaacatcc tgcggaacgg tctgctgcag gcgggcgatg cggacatcgc gcgaccggac
     5401 gatctcacct tcatccggtt ccagatcgcc gctgacgatt ttgagcagcg tcgattttcc
     5461 ctcgccgttg cgtccgagca gacagatgcg ttccccgggt tcgatctgga gcgtcacgcc
     5521 gtcgagcagc ggaggtccgc cgaacgcaat atgaagattc tgtaaactga gtagcgccat
     5581 aagaaggcgg agaaggtacc agtccggccg gctgattcaa acagcattta tacaaaatga
     5641 cgccccagaa gttcttcgac ctgatcgcgc cagacggcat catacgtccg gcctttctgc
     5701 agccgcacaa aaatccggcg cgcagcaatc tggccgaaca gctccgccga cgcaaagcga
     5761 tccagcaggt gcagcggctg ttttttaatc gtcgcaatca gcgcttccag caattcgatc
     5821 ggctcttcgc gctccggaca gagaataaac tgccaaagca catccggctc gtcggcgatc
     5881 acctgctcaa tcatccggca gatttctttt cggcgtccgt gcaaaccgcc gccgcggaat
     5941 atcagcgcac ggcggttcag cccggcctgt tctttaaaaa gcccggtcag ccggtaaccg
     6001 gtaaaacgcg aggttatcgg atcagcgggc atatcgtcgc tgtcgtgcag gaacgcttcg
     6061 atccggcgca tatcctcctc cggcatcgtc gaagtggaaa atacgccgta cggcggaagc
     6121 ggcatcgctt tcattccgta ctgttccgcc tgcccgcgca gatcggttcc cggcaaaaga
     6181 agcgtttgca tacactgaac agtaatgccc ggcccgaggc cgcgcgccca ctcaatgctg
     6241 cgaagcacat cgtcgaacgt ctgttccggc agaccgtaca tcagatcgag cgtcacacga
     6301 acgcctgcgc cggtcagcag ccggatgccg cgctcaacct gcgcgatatt cgccggacgg
     6361 cggatggtac gaagcacttt tggatcggta ctctgcacac caacttcgac atcgatgaaa
     6421 ttgactttcg ccatcagcgc cgcctgctcc ggcgtcagcg tatcggcacg cagttcggcg
     6481 aaaaacccca gcttccggtc ggcgttgatt tccgccagcc gttccaataa ccggacaaag
     6541 tcgccgcggg cattgaacgt tggatcgaca aagcggattt cccgcgcgcc ggcagcgcgc
     6601 aactgccgga cgcgctcagc ggcggcttcc gccggaagtg cgcggacacc tttctgcaaa
     6661 tgatgataac ggcagtaggt gcaggtcagc ggacagccgc gcgaagtttc gatgtaaacc
     6721 gccgggcgcg ctttgatgca gtccatcagc gccgacgccg gaacctgcac cgaagccaga
     6781 tcgacctgcg gcggcggctt gcttccgcgc cgccagccct tatccgacga aatcagcacg
     6841 ttgtcatacg ccggcagttt gccggtgcgg attcctttca gaatttccgg aaaaaccgct
     6901 tcgccttcgc cggttaccag cgcgttaaaa accggatcct ccagcagaaa cggatgatcc
     6961 tccgcaatct ccggcccgcc cgctatcagg aaaagatcag gacgcgcttc ccgcaaccgg
     7021 ctcgccagcc gaagactgcg ctccacattc caaagacaaa gcgtgaaggc aacggcctcg
     7081 acttcgcttg ccagaacttc ttcaaacaga cgggcgttgt ctaattcatc ccactttgcc
     7141 ggagccggaa cgacatccca gaattcgcct tcgggatgct gtgacagcgc atgcgacagc
     7201 cagagcgccg cgaacggaaa attttcattc ggcgtcgacg tgtcattgtc cagcagcgga
     7261 agctggatga ataaaatttt ctttttcatc aggagtcttt tcccacgaat cccacgaatg
     7321 aacacgaata atccgccggc tggattcgtg aaaattcgtg tgattgcggg cggttcttat
     7381 tgttgggtcg ttagtggagg ccgaacatgc ggcggatagc cgggaataat ttccgaaaga
     7441 gcctgcttga tgcgggcgat ggcgaacgga ccgtttgttc gtgcattcac gacatccacc
     7501 gccgcctgat tttccggcga ctgttcctgt tcaatccgca ggtacggcag cggatcaata
     7561 ttagcggaag cactgacaaa tcctttcgca cccgccgcgt aggcctcgga aattttgcgg
     7621 tcgccgccaa caaagtagcg cggcgtcgcc ggaatcagcg caagatccgc cgaagaatcc
     7681 ttcagccccc agtgcggaac ctgcttcagg atttccggcg tcagcggatt gccggtatgc
     7741 ttcggaaagt tgtagagaat cagcggaagc gcggtggact ccgccagctc attaaaccag
     7801 cggacaagtc cggagccggg cgcaccggca aaataaaacg gcggcagcga caccagaaaa
     7861 tccgcgccga tttcttccgc gacgtgagcg gcctcgccgc acgatgcgag cgccggactg
     7921 ccggcatggg cgataatgat tccgtcccag ttttcgcggg cggtctgaag cagctgcaac
     7981 tgctcggctg gcgacagcga gaaaaactcg ccggtcgtgc cgttcaccag cagattgcgt
     8041 acgccgcatt ccgccagcca gtcgagatgc gccagaaacg c
//
LOCUS       DAOPZO010000087         8056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_10708_length_8056_cov_7.777403, whole genome shotgun sequence.
ACCESSION   DAOPZO010000087 DAOPZO010000000
VERSION     DAOPZO010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 8056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8056
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10708_length_8056_cov_7.777403"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..1971
                     /locus_tag="PKI68_06595"
     CDS             274..1971
                     /locus_tag="PKI68_06595"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HNX53396.1"
                     /translation="MSNSKETFVDNLINKMTLEQKVGQCFVIGFCGTVVTPAILKRIR
                     KIKPAGIRAGLTFRAKTALHDPYATSEVFAHRVLREPKGSVKDIIPGLLPPHCTNEEY
                     CTFLNTMKQEALKDGLGIPLHVTMDMEGDVSCDYTRGGIFNFPFPMGASASGDPKIAY
                     DMAWATGRQLHALGVNWLHSPVLDTNTEPNNPEIGTRSYGENAETVIKFGTEGFKGFK
                     AAGIITTGKHFPGRGESVTDAHRGLPVIDMPRAKMEEHLKPFKAMVDAGIPCIMTAHT
                     VYPCLDPDNAATLSKKILTDLLKNEWGFKGCVTTDDITMGGIVEKYEVADACVVALNA
                     GCDLILLRDEAPLIDEVYEKVVQAVKDGKLSEERVNDAIRRSLTVKYEYGLFENGNVR
                     DVAKAGDGIKDPKVAKIAKASADAAVKVIRDEQKVLPLKRGKSVLVIEQRCPLHYRTD
                     SQKCHPGIFWENFFKYTEEAGQVEVEMSPTEYDMKRVLKRVNEADVIVTTNYFDRRAH
                     AKGNFIDQILAATTKPVIVVTNSPYPFTVSDDYKTVICTYGVAPESLEAAVRTLYGE"
     gene            2113..3888
                     /locus_tag="PKI68_06600"
     CDS             2113..3888
                     /locus_tag="PKI68_06600"
                     /inference="COORDINATES: protein motif:HMM:NF021534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HNX53397.1"
                     /translation="MPGFDLPIARNPLKTRADVQEAVRQLCAPLLPHYSPGRARVHLG
                     DTTTNYGPLRCEMEAFARPFWGLMPLAAGGGDSEFWDICRTGLLNGTDPKHPEYWGTL
                     VDVDQLAVEMPPIALALALAPEIFWKPFTVAQRQQIVTWMDQINHHTMSDNNWRAFPV
                     LVNASFKKAGMPYNAEVMETGLRRLEDFYLSEGWYSDGAAPRMDYYIPFAMHFYSLIY
                     AKLMGADDPKRAALFKERATVFAKDYLHWFTADGAALPFGRSLTYRFAQGAFWAALAF
                     ADVEALPWGEIKGLYLRHLRYWFSQPIFTPDGLMSVGYLYPNLLMSEFYNGPGSPYWA
                     MKAFLALAVPADHPFWTSEELPFPQAPAMSVQKLALMHIASSPAHDHLVALTSGQWCA
                     YDFGHTAEKYSKFAYSTHFGFSVSREATGLMKGAYDSVLALSDGEDHWRVRRKCEKVE
                     ITDNAVISWWKPWPDVEIETRLEFAYPWHIRTHRIKTGRALETAEGAFAAAREDGLSP
                     LKNLSDCPQGAFYGGSGILDLNGQRQAILQEPMPNTNIMVPRTVIPMLTGRIEPGEHC
                     LVCAVLGEPSAAAAAAAWKNPPAAR"
     gene            4034..5665
                     /locus_tag="PKI68_06605"
     CDS             4034..5665
                     /locus_tag="PKI68_06605"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF010322.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNX53398.1"
                     /translation="MQRRSFIQMGGLASGMLLFGCRAQKVIPKKRMNILFIMDDQHRG
                     DCIGAAGADWLNTPNLDRLAAEGALFNRAYSSTPSCLPARAAILTGLRPWNHGLLSFL
                     AIPNKYKVEMPQLFTDAGYRTHVAGKNHFYGAKHGYQTTEVEEIFLDKDADGFYKDDY
                     LRWLQQNHPEVLKSVTDQNFYNDHRDLAWKYAEETHPTHWTAERAKAFLREYKGEAPW
                     FLKFSLHRPHCPLDPPRRLVDKYLQKTLPSPAVGPWAEKAYGEAVGSMQQAPKAAIGH
                     FPDKEIHESRAAYYASIEYVDEKIGEVLEVLKERGELENTFILFTSDHGDMMGDNYRW
                     QKCCPYEPSARVPMLIRWPAAMNLPVRRGQQFDHLVELRDIMPTFLDAAGLPVPGNID
                     GKSMVQILRDSACPWRDTLEIEHGSQYFSEDAYVALTDKRYKYIYFTQTGDQQLFDLK
                     KDPKELNNITDPVLLAAWRKRMVAFLSERGEEWVKNGDLVLQTRRMLYSPNFKKNMLE
                     PADEQVEKRFNPSTEPPTLKEYIRQLGYSGKFTGV"
     gene            5962..6981
                     /locus_tag="PKI68_06610"
     CDS             5962..6981
                     /locus_tag="PKI68_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNX53399.1"
                     /translation="MQDQKYVAIKDIAKACGVSTTTVSLALRNHPRISEDTRKRISET
                     AERMNYRRNPMISALMTNLYQSRKFPSAVPLAVLYTHELSLIEENSYHQKLWQGMSNR
                     AEELGFKLEKFCLNRKITGKRMTQILTARGINGVIIPPLLRAGGHLSLDWEQFSVLAL
                     GYSLLSPNFHRVCPNQYQGIRTVMRQLKHVGFSRPGMVLMNESDLRTAHLWSSGFYGF
                     EHAVKKTGIIPVLEYVNSDKGAVMDWYRKYTPDVIIGSEPDVLELFRSAGLKIPEEVG
                     FVTLSRWSSENSIAGIDENEEMLGRAAIEQLVHLVYYNQRGCPECPYVIQVPCKWGAG
                     ITIRK"
     gene            7470..>8056
                     /locus_tag="PKI68_06615"
     CDS             7470..>8056
                     /locus_tag="PKI68_06615"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HNX53400.1"
                     /translation="MRFNSRVVMMWAAGALVCGAQSGSWDPVQMQKEQANRFLARGPG
                     GGTGVQMLEAKSLRDGQNRTYPYPVKGHKVLTNPVVFTWPMSDYVYAEVYPRTQLVKP
                     LDQYDRYGFQMSRSPDFPEVSTRTVTGLRLPFYNPHRKMEPGVWFWRYRVENAAGPQA
                     WSKTYEMTMDPAAPIFESPTADEAFAMLPRSFPRI"
BASE COUNT         1763 a         2211 c         2359 g         1723 t
ORIGIN      
        1 ccgcgcgatg cggaatctct gtaaaaagct ccgggaaaat cccggagctt tttttgcatg
       61 tatagatatg tttttagacg aacggggcga ggtgtgtcct accggacggc cttgtcaacc
      121 gtttttgagc gattccaaat aagtttacgg taaaactgaa cctccggtgt ttgtcaattg
      181 gtttgccatc cggtggtcgg cacaatcacc aactgaccgt agttccttgt gttgaaggaa
      241 ctcaactaaa acaggtagag ataaggatat agtatgagca actcgaaaga gacatttgta
      301 gataatctga tcaataagat gaccctggag cagaaggtag gccagtgctt tgttatcggg
      361 ttttgcggaa ccgtggtaac tccggcgatt ctgaagcgca ttcgcaagat caagccggcg
      421 ggtatccgcg cgggtctgac cttccgcgcc aaaaccgctc tgcacgaccc ttacgccacc
      481 agcgaagtgt tcgctcaccg cgtactgcgc gagcccaaag gcagcgtcaa ggacatcatc
      541 ccgggcctgt tgccgccgca ctgcaccaat gaagaatact gcaccttcct gaacacgatg
      601 aagcaggaag ctctcaaaga cggcctcggc atcccgctgc acgtcacgat ggacatggaa
      661 ggcgacgtca gctgcgacta cacccgcggc ggcatcttca acttcccgtt cccgatgggc
      721 gccagcgctt ccggcgatcc gaagatagcg tatgacatgg cctgggccac aggccgtcag
      781 ctgcacgcgc tgggcgtgaa ctggctgcac tcgccggtgc tcgacaccaa caccgagccg
      841 aacaacccgg aaatcggtac ccgcagctac ggcgaaaacg ccgaaacggt catcaagttc
      901 ggcaccgaag gcttcaaggg cttcaaagcg gccggcatca tcaccaccgg taagcacttc
      961 ccgggtcgcg gcgaatccgt taccgacgcc catcgcggtc tgccggtcat cgatatgccg
     1021 cgcgcgaaga tggaagagca tctcaagccg ttcaaggcga tggtcgatgc aggcatcccg
     1081 tgcatcatga cggctcacac cgtttatcct tgcctggatc cggacaacgc cgccaccctc
     1141 tccaagaaga tcctgaccga cctcctcaag aacgagtggg gcttcaaggg ctgcgtcacc
     1201 accgacgata tcaccatggg cggtattgtt gagaagtacg aagtcgccga cgcttgcgtc
     1261 gtggcgctga acgccggctg cgacctgatc ctgctgcgcg acgaagctcc gttgatcgac
     1321 gaagtgtacg agaaggttgt ccaggcagtc aaagacggca agctctccga agaacgcgtg
     1381 aacgacgcga tccgccgctc gctcaccgtc aaatacgaat acggcctgtt cgaaaacggc
     1441 aacgttcgcg acgtggcgaa agccggcgac ggtatcaaag acccgaaagt tgcgaagatc
     1501 gctaaagcca gcgccgacgc tgcggtcaag gtcatccgcg acgaacagaa agttcttccg
     1561 ctgaagcgcg gcaagagcgt gctggtcatc gaacagcgct gcccgctgca ctaccgcacc
     1621 gactcgcaga aatgccatcc tggcatcttc tgggaaaact tcttcaagta caccgaagaa
     1681 gccggacagg tggaagtgga aatgagcccg accgagtacg acatgaagcg cgtgctcaag
     1741 cgcgtcaacg aagcggatgt cattgtaacc accaactact tcgaccgccg tgcacatgcg
     1801 aagggcaact tcatcgatca gattctggct gctacaacca agccggtgat cgtggttacg
     1861 aactccccgt atccgttcac ggtatcggac gactacaaga ccgtgatctg cacctacggt
     1921 gtggctccgg agtcgctcga agcggccgtt cgtaccctct acggcgagta agccgcagcc
     1981 gcagccgggc ctgcggctgt caccgtggcg gcgggaggtt tctcccgccg cccggctgtc
     2041 aggccccggc gtttcatttt ttctgttcat ctttttccgc tgttgagaaa atatagagtt
     2101 aaggagccga gaatgcctgg ttttgatctt ccgattgccc gtaatcccct taaaacccgc
     2161 gccgatgtgc aggaagccgt ccgccagctc tgcgcgccgc tgctgccgca ttacagcccc
     2221 ggccgcgccc gcgtacacct cggcgacacc accaccaact acggtccgct ccgctgcgaa
     2281 atggaagcct ttgcccgccc gttctggggc ctgatgccgc tggctgccgg cggcggcgac
     2341 tccgaattct gggatatctg ccgcaccggc ctgctcaacg gaaccgatcc gaagcacccg
     2401 gaatactggg gcacgctggt ggatgttgac cagctggcgg tcgaaatgcc gccgatcgct
     2461 ctggcgctgg ctctggcgcc cgaaattttc tggaagcctt tcacggtcgc ccagcgtcag
     2521 cagatcgtga cgtggatgga tcagatcaat caccacacga tgtccgacaa taactggcgg
     2581 gctttcccgg tgctggtgaa tgcctcgttc aaaaaagcgg gcatgcccta taacgccgaa
     2641 gtgatggaaa ccggtctgcg ccgcctcgaa gatttctacc tgagcgaagg ctggtattcc
     2701 gacggtgccg cgccgcgcat ggactactac atcccgttcg cgatgcattt ctattcgctg
     2761 atctatgcca agctgatggg cgccgacgat ccgaagcgcg ccgcgctctt caaagaacgc
     2821 gccacggttt tcgccaaaga ttatctgcac tggttcaccg ccgacggcgc cgcgctgccg
     2881 ttcggccgca gtctgaccta tcgctttgca cagggcgcct tctgggccgc gctggcgttt
     2941 gccgatgtcg aagcgctgcc gtggggcgaa atcaaggggc tctatctgcg ccacttgcgc
     3001 tactggttca gccagccgat tttcacgccc gacggtctga tgtcggtcgg gtatctctat
     3061 ccgaacctgc tgatgagcga gttctacaac ggtccgggtt caccgtactg ggcgatgaaa
     3121 gcctttctgg cgctggccgt tccggcggat catccgttct ggacatccga agaactgccg
     3181 ttcccgcagg ctccggcgat gagcgtgcag aaactggcgc tgatgcacat cgccagctcg
     3241 ccggcgcacg accacctggt cgcgctgacc tccggccagt ggtgtgccta tgacttcggt
     3301 cataccgccg aaaaatattc gaaatttgcc tattcgacgc acttcgggtt cagcgtctcg
     3361 cgcgaagcga ccggcctgat gaagggcgcc tatgactccg tgctggcgct cagcgacggc
     3421 gaagatcact ggcgcgttcg acggaagtgc gaaaaggtgg agatcaccga taacgcagtg
     3481 atttcgtggt ggaagccgtg gccggatgtt gaaatcgaaa cgcgtctgga gttcgcctat
     3541 ccgtggcata tccgcacgca tcgcatcaaa accggccggg cgctggaaac ggcggaaggc
     3601 gcttttgccg cggctcgcga agacggtctg tcgccgctca agaatctgtc cgattgcccg
     3661 cagggcgcgt tctacggcgg cagcggcatt ctcgacctga acggtcagcg ccaggcgatc
     3721 ctgcaggagc cgatgccgaa cacgaatatc atggttccgc gcacggtgat cccgatgctg
     3781 acgggccgca tcgagcccgg cgaacactgc ctggtgtgcg cggtgctggg cgagccgtcg
     3841 gccgccgccg cggccgccgc ctggaaaaat ccgccggcag cccgctagcg gacgggcggg
     3901 tgctccgggg ttccggagca ctcgccggga cggaagaacc ggcgtatatt gtttcagcga
     3961 ataaaaaggc tgttattttt aatgtcttga tttgcagttg ctgtggcaga atgaactgcg
     4021 acgggagggt gatatgcaac gcagaagttt tatacagatg ggcgggctgg cgtccggaat
     4081 gctgctcttc ggatgccgcg cgcagaaagt gattccgaaa aaacggatga atattctgtt
     4141 tatcatggac gaccagcacc ggggggattg catcggcgcc gcgggtgccg attggctgaa
     4201 tacgccgaat ctcgaccgcc tggccgcaga aggcgctttg tttaaccggg cctattcatc
     4261 gacgccgtcc tgccttcctg cccgggcggc tattctgacc gggctgcgcc cctggaatca
     4321 cgggctgctt tcttttctgg cgatcccgaa caagtacaag gttgaaatgc cgcagctgtt
     4381 tactgatgcg ggctatcgca ctcacgttgc agggaagaac catttttacg gagccaagca
     4441 cggctatcag acgacggaag tcgaagagat ctttctggat aaagatgccg acgggttcta
     4501 caaggacgat tacctgcgct ggctgcagca gaatcatccc gaagtgctca agagtgtgac
     4561 ggatcagaat ttctataacg accatcgcga tctggcgtgg aaatatgcgg aagaaaccca
     4621 tccgacgcac tggaccgccg agcgggccaa ggcgtttctg cgggagtata aaggcgaggc
     4681 gccgtggttt ttgaagttct cgctgcatcg tccgcattgt ccgctcgatc cgccgcgccg
     4741 gctggttgat aaatatcttc agaaaacact gccttcgccg gcggtcgggc cgtgggcgga
     4801 gaaggcctat ggcgaggcgg tcggttccat gcagcaggcg cccaaggcgg ccattggcca
     4861 ttttcctgat aaagaaatcc atgagtcccg cgccgcttac tatgcatcca ttgagtatgt
     4921 ggatgagaaa atcggtgaag tgcttgaggt gctgaaggag cgcggcgagc ttgagaatac
     4981 attcattctg ttcacctccg atcacggcga tatgatggga gataactatc gctggcagaa
     5041 gtgctgcccg tatgaaccga gtgcgcgcgt gccgatgctg atccgctggc cggcggcgat
     5101 gaaccttccg gtccggcgcg gacagcagtt cgatcatctg gttgagctgc gggatattat
     5161 gccgacattt cttgatgccg cgggactgcc cgttcccgga aacattgacg gcaagagcat
     5221 ggttcaaatt ctgcgcgatt cggcgtgccc gtggcgggat acgctggaga ttgaacacgg
     5281 ctcgcagtac ttcagtgaag atgcctatgt ggcactgacg gataagcggt ataagtatat
     5341 ttacttcact cagaccggcg atcagcagct gtttgatctc aaaaaggatc cgaaggagct
     5401 gaataatatc accgacccgg tcctgctggc ggcgtggcgc aaacggatgg tggcgtttct
     5461 gtccgagcgc ggtgaagagt gggtgaagaa cggcgatctg gtgctgcaga ccaggcggat
     5521 gctttacagt cctaacttca aaaagaatat gctcgaaccg gccgatgagc aggtggagaa
     5581 gcggtttaat ccgtccaccg agccgccaac gctgaaagaa tatatccggc aattgggata
     5641 ttccggaaaa tttaccgggg tttagccggg atcggaatct gaggccgttc ttttcagccg
     5701 cccgccggta tttgagaccc ggcaggagag tatggcccgg cacatatccg gcattgtata
     5761 tttgatgtgt tttcgatagc gtactcggaa aatccaaacc ttgttcgcga aacctccggc
     5821 tgtttgtcgc gcgaatgaat gtgttcctcg gcaggagttg gggaggaatg cggatttctc
     5881 gtccgccact tgtggcgtga ggccggcaga gaggcttcgt gatttgtcgc ggagattgtc
     5941 ggcccaaagg gagtttatgc tatgcaagac cagaaatatg tcgctatcaa agatatagct
     6001 aaagcttgcg gtgtttccac cacaaccgtt tcgctggctt tacggaacca tccgcgtata
     6061 tcagaagata cgagaaaacg catttctgag acggcagagc gtatgaatta tcgccgtaat
     6121 ccgatgatct ctgcgcttat gaccaatttg tatcagtcgc gtaaatttcc ttctgcagtc
     6181 ccgctggcgg tgctttatac gcacgaatta tccctcatag aagaaaacag ttatcatcaa
     6241 aaactttggc aggggatgtc gaaccgggcc gaagaactgg ggttcaagct ggagaaattt
     6301 tgcttaaaca ggaaaataac cggtaaacga atgacccaga tattgactgc tcgcggaatc
     6361 aatggagtca ttattcctcc gttgttgcgg gctggcgggc atttaagcct cgactgggaa
     6421 cagttttcag ttttggcgct gggatattcc ttgctgagtc cgaattttca ccgggtttgt
     6481 cccaatcaat atcaggggat ccggaccgtc atgcggcagc tcaaacatgt cggattttcc
     6541 cgcccgggaa tggttttgat gaatgaaagt gatttgcgca cggcgcacct ttggagttca
     6601 gggttttatg ggtttgagca tgccgtcaaa aaaaccggaa tcattccagt gctggaatac
     6661 gtcaattcgg ataaaggcgc ggttatggat tggtaccgga agtatacgcc ggacgtgatt
     6721 atcggctcag agccggatgt gctggagtta ttccggtcgg caggacttaa aattccggag
     6781 gaagtcgggt ttgtcacact ttcgcgatgg tcttcggaaa actccattgc cggtattgat
     6841 gaaaatgaag aaatgctggg gcgggcggcc attgaacagc tggtacacct tgtgtattac
     6901 aaccagcgag gctgtcctga gtgtccctat gtaattcagg ttccttgtaa atggggcgcc
     6961 gggataacga tcaggaaata gactgattca actatcgaac gggacgaagt gaagtttccc
     7021 gtcctcctgt gtttttgttc ccgctgtcag gcgggccctg tcgggtttta catctgcctc
     7081 atcaatgaag cccttcatgc agaagatgct gcattctttt tgaaatgatc tggttcaaaa
     7141 atcagcacct gaaaacggtg cttataaaca actcaagttt acgaggcaac tgaactttgc
     7201 ctgcagtctg ttcgttttct ccaagcaatc atttgtataa taaaaattcg tgtataagga
     7261 tacagattgg ggtttgttgc tgtatggcga acaggcaaaa taatttacag tctgccaact
     7321 gttcgcgcag cacattgctg tgcgaaaaaa cgacggggcg caggagacgg ccgatgctct
     7381 gccgctgatg gcgcgtcaaa aaagagcatc gccgttttgc cggtgcaagg cggcgtttca
     7441 gtttatctaa ttatgaaatg aggtgcagga tgcgattcaa tagtcgtgtg gtgatgatgt
     7501 gggcggccgg cgctttggtg tgcggggctc agagcggaag ttgggatccc gtgcagatgc
     7561 agaaagagca ggcaaatcgt tttctggccc ggggaccggg cggcgggacg ggcgtgcaga
     7621 tgctggaagc gaagagcttg cgcgacgggc agaaccgcac ctatccatac ccggtcaagg
     7681 ggcacaaggt gctgaccaac cccgtggtgt ttacctggcc gatgagcgat tatgtctacg
     7741 ccgaggtcta tccccgcact caactggtca agccgctgga ccagtatgac cgctacggct
     7801 tccagatgag ccgctcgccg gatttcccgg aagtctccac gcgcaccgtg accggtctgc
     7861 gcctgccgtt ttataatccc caccgcaaga tggagccggg cgtctggttc tggcgctacc
     7921 gcgtggaaaa cgccgccggc ccgcaggcgt ggtccaaaac ctatgaaatg acgatggatc
     7981 cggccgcccc gatttttgaa tcgcccacgg ccgacgaggc gttcgccatg ctgccgcgca
     8041 gcttcccgcg catcta
//
LOCUS       DAOPZO010000088         7779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_11269_length_7779_cov_9.089979, whole genome shotgun sequence.
ACCESSION   DAOPZO010000088 DAOPZO010000000
VERSION     DAOPZO010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11269_length_7779_cov_9.089979"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PKI68_06620"
     CDS             <1..258
                     /locus_tag="PKI68_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53401.1"
                     /translation="GNVMMQYIDTGDVFAGNSDVIPNGYEVRYLCQGRRDGGGTYRNR
                     LGCLVYNPNIMRIGIMHHDAICRRVVKKGFKGRLGSHTKTA"
     gene            complement(262..627)
                     /locus_tag="PKI68_06625"
     CDS             complement(262..627)
                     /locus_tag="PKI68_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53402.1"
                     /translation="MNCNRLSACRPRTPPSTPATSAAKPRRTSDGMSSVDAVRTRLLR
                     LKIFPDRVKILATRNTKRHEKVERDLRARLPARQKKTRSAIAFHPMIPFADDVQYFIK
                     AGWKPAVRIAGFQPALSLD"
     gene            complement(617..2857)
                     /locus_tag="PKI68_06630"
     CDS             complement(617..2857)
                     /locus_tag="PKI68_06630"
                     /inference="COORDINATES: protein motif:HMM:NF016945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase"
                     /protein_id="HNX53403.1"
                     /translation="MRTVIKLLSLLMISISLAAAAAAAPEKAEAAGPEAVKALIQRMT
                     PGCADRFAVEFIPPADGKDVYEIESGEPGRIVLRGSSGVAAASAYYHYLKEFCRCHVS
                     WNGDQLNLPAVLPAVSRKIRVVSPVQTRLAYNYCTHGYSMVWWDWAQWERELDWLALH
                     GINTALIIQGQDAVWQNTFARFGYTKEEIRQWLCSPIYQPWQFMQNMERVEPPPQSLI
                     DKRVELGRKIISRCRELGIEPVLQGYFGMLPSGFKEKYPDARIVAQGGWAGGNRRPDM
                     LHIKDPRFEPIAIAFLEEQRKLFGECRMFAADPFHEGGKPGDMKRGDAYKAIQEIILK
                     FDPNAVVVKQCWQTSNKEMFDAGNKSRTLALDLYCDNMPFWPKCQGYDGTPWVWSLLH
                     NFGGNIGMEGNLPRMANDIAAVFSDSGRGALTGLAIVPEGTQQNPVVYELMTEMGWRG
                     APADIEAWVRNYIHSRYGKANPSAAEAWNLMLQSNYAITSGQSPVSSVIVAAPRLDPN
                     IKGRKWSSGSKILYPKHKLYSAWKKMQAASTDLGAADTYRFDLADVARQSLCNLARDV
                     YDRMSDAYKARDLEGFKKQSATLLGIIGDLETLTATRREWLMGKWVADALQWGGTEDE
                     KTYLDRNARLLLTTWLPNPNTALSDYASREWSGLLGEYYYTRWSLFVKALTNDLEGTE
                     KFSMDKYAKDRAAFEVKWINGRSPLRSAPAGDTVAVSAALLAKYGPLMDEIQPAPLTP
                     KAANEL"
     assembly_gap    2978..3077
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3078..4570
                     /gene="rlmD"
                     /locus_tag="PKI68_06635"
     CDS             <3078..4570
                     /gene="rlmD"
                     /locus_tag="PKI68_06635"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNX53404.1"
                     /translation="GGWFRKSFDRAGVPWFNSPLMYKKGQMIETELFDTADKNRCVGK
                     LEDGICVFVEGTAAVGDTVRAEIYKTKKRYLVASLKEILKFSDRRTAPRCKYFGLCGG
                     CKWQHLDYSEQLRVKRKQVLDALEHLGGFEGPEVADCIPAEEIFGYRNKIDMDFTDRR
                     YLTADEMNIPEAELSKPLDFALGFHAPGCPFKAIDIDHCDLASEEMNLAVETVRRFCR
                     DRKLSIYSTFTHEGFLRNLVVRRGERTGELMVNLITLTHDPELMRELCTALQAALGAR
                     LTTFINATTDRKNMVAFGDQEYVLCGEGSISDRLGEYTYRISPNSFFQTNTVQAEKLY
                     NEILKAARLTGSETVYDLFCGTGSIALFAAKHCGKVLGIELVEAAVADARKNAQEHGA
                     ANCSFRQMDLMHFGKIRAELEAFGLPDVVITDPPRAGMHPKAVEMLRELAPPVVIYVS
                     CNPASLARDGQLLCEGGLYRLISTQPVDLFPQTNHVESVARFERVS"
     gene            complement(4577..5113)
                     /locus_tag="PKI68_06640"
     CDS             complement(4577..5113)
                     /locus_tag="PKI68_06640"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNX53405.1"
                     /translation="MARKSFPLSKVYGLLEPGPVVLVTTARDGRANIMTMSWQTMMEF
                     EPPLIGCVISDRSYSFGLLKATGECVINIPSVEIAEKVAGCGNTSGAKTDKFKKFGLT
                     PLPASTAGAPLIKECFANLECRVIDTGMVDKYCLFILQVVKAWIDPDVKNPRTIHHLG
                     RGNFTVDGKRIKLKSKMK"
     gene            5394..6233
                     /locus_tag="PKI68_06645"
     CDS             5394..6233
                     /locus_tag="PKI68_06645"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNX53406.1"
                     /translation="MVWKKEFQFVALALLLSAAGGYAETNQTAAADDKHPAACPHCGF
                     TPTNPVAAAGSTVTNRASTNDWKSSVYGGFSAKSGNTVERSYNYGADLQRVGKIYRGT
                     LQADGRYSRTEDQVTTSKSEASGELRRMLDEHWFAYGVLSATHDDLKDLSYRVKTGPG
                     IGYYFVDSKELTADVSSGPLYVHEKTADGASGYLAWRFAQGFNWQITGTFRVWTLTEA
                     FVDTTDTAAYTIAAKAGVESKISGNLSLVVMIEDDYDSLPETQDEIEKNDFEISTGLR
                     YSF"
     gene            6251..>7779
                     /gene="metG"
                     /locus_tag="PKI68_06650"
     CDS             6251..>7779
                     /gene="metG"
                     /locus_tag="PKI68_06650"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNX53407.1"
                     /translation="MTKKSYYVTTPIYYVNDKPHIGHAYTTILADVLASYHRLLGTPT
                     WFLTGTDEHGQKVQQAADKNGLTPQQQCDQTVVRFQELWKRLEITNDDFIRTTEERHK
                     VIVQEVLQDLYDRDEIYRAEYEGWYCVGCERFFAEKDLVDGNCPECGRKVDAIVETNY
                     FFRMSKYQEWLIDYIETHPEFIQPDFRANETLGFLKTKKLQDLCISRPKSRLAWGIEL
                     PFDKEFVTYVWFDALLNYISAIGYKRDDAKFNQWWPATHQLIGKDILTTHTVYWPTML
                     KAMNVPLPKTVFAHGWWLTGRTKMSKSLGNVVNPMEMVDRYGVDAFRYFLIAEMTLGQ
                     DASFTEEAFIRRYNSDLANDLGNLMSRTVNMLGRYCDGTFPALGSGALDGAPEQELWT
                     AVQQAVVEMESSIGTMHLNGGLAAVLAAVRKVNQYFEIKQPWTQAKEGKTEAVGVTLA
                     LAAESLRVCAALLYPVMPEKMSALRAAFGLGKPELSQLRTFGVIKAGTVIGNPGILFP
                     RV"
BASE COUNT         1545 a         2298 c         2262 g         1574 t
ORIGIN      
        1 ggcaatgtca tgatgcaata tattgatacc ggtgacgtat ttgccggaaa ttccgatgtt
       61 ataccaaatg ggtatgaggt tcggtattta tgtcaaggcc gccgggacgg cggcggtacg
      121 tatcgcaacc gtctcgggtg ccttgtctat aacccgaata tcatgcgcat tggtattatg
      181 catcatgacg caatttgcag gcgggtggtc aaaaagggtt ttaaggggag attaggaagt
      241 cataccaaaa ccgcgtgatg atcagtctaa agacaaggcc ggctggaagc cggcgatacg
      301 taccgcaggc ttccagcctg ccttgataaa atactggaca tcatccgcaa agggtatcat
      361 tgggtggaac gcgatcgccg agcgcgtttt cttctgccgg gccggtaagc gcgcccggag
      421 gtcgcgctcc accttctcgt gcctttttgt gtttcttgtg gctaaaattt taacacggtc
      481 gggaaagatt ttaagacgga gcagccgtgt tctcaccgcg tccacgctgg acatcccgtc
      541 ggacgttctt cgcggcttgg cggccgaggt cgccggcgtg gacggcgggg tgcgcgggcg
      601 gcacgccgac aatcggttac agttcattcg ccgctttagg cgtcagcggt gcgggctgga
      661 tttcatccat tagcggcccg tatttggcaa gcagcgccgc cgagaccgcg accgtgtcgc
      721 cggccggggc ggagcggagg ggcgagcgcc cgttgatcca tttcacctcg aaggccgctc
      781 ggtctttggc gtacttgtcc atgctgaact tttcggtgcc ctcgagatcg ttggtcagcg
      841 ctttcacaaa caggctccac cgggtgtagt aatactcgcc caggagcccg ctccattccc
      901 ggcttgcgta gtcactgaga gcggtgttcg gattgggcag ccacgttgtg aggagcagtc
      961 tggcgttgcg gtcaagatag gtcttttcat cttccgtgcc gccccattgc agcgcatcgg
     1021 ccacccattt gcccatcagc cattcgcggc gggttgcggt cagcgtctca agatccccga
     1081 tgattcccag cagcgtggcg gattgttttt tgaacccctc cagatcccgc gctttgtatg
     1141 cgtccgacat tctgtcgtag acatcccgcg cgagattgca cagcgactgt cttgcgacgt
     1201 ccgccagatc gaatcggtag gtgtcagccg cccccagatc ggtcgaggcc gcctgcattt
     1261 tcttccaggc ggaatagagt ttgtgtttcg gatagaggat tttcgagccc gagctccatt
     1321 tgcggccttt gatattcgga tccagtcttg gggccgcgac aatcacggag ctgacagggc
     1381 tctgtccgga ggttatggca tagttgcttt gcagcatcag gttccaagcc tcggcagcgc
     1441 tggggttcgc tttgccgtaa cgggagtgta tgtaattacg aacccacgcc tcgatgtcag
     1501 ccggagcgcc gcgccagccc atctcggtca tcaattcgta tacgaccgga ttctgttggg
     1561 ttccttccgg aacaatggcg aggcctgtca gcgcgcctct gccgctgtcc gaaaatacag
     1621 cggcgatgtc gttggccatt cgcggcagat tgccctccat cccgatgttg ccgccgaagt
     1681 tgtgaagcag gctccacacc cacggcgttc cgtcatagcc ttggcatttg ggccagaacg
     1741 gcatgttgtc gcagtacaga tcgagcgcca gagtcctgga cttgttgccg gcatcgaaca
     1801 tctccttatt ggaggtctgc cagcactgct tgacgacaac ggcgttcggg tcgaatttca
     1861 ggatgatctc ctggatcgct ttatacgcat cgccccgctt catatctccc ggcttcccgc
     1921 cttcgtgaaa cgggtccgcc gcaaacatcc ggcactcccc gaacagtttg cgctgctcct
     1981 ccaggaaggc gatggcgatc ggctcaaagc gcgggtcctt gatgtgcagc atatcgggtc
     2041 tccggttgcc gccggcccag ccgccctgtg ccacgatccg ggcgtcaggg tatttctctt
     2101 tgaaccctga cggcagcatc ccgaaatagc cctgcaatac aggttctatt ccgagttcgc
     2161 ggcatcggct gatgatcttt ctgccgagct cgacccgctt gtcgatcagg ctttgcggcg
     2221 gcggctccac ccgctccatg ttctgcatga actgccaggg ctgatagatc ggggagcaga
     2281 gccactgccg gatttcctct ttggtatacc cgaaacgcgc gaacgtattc tgccaaaccg
     2341 catcctggcc ctggatgatg agcgcggtat tgatgccgtg cagggccagc cagtccagtt
     2401 ctctctccca ttgcgcccag tcccaccaca ccatcgaata tccgtgggtg cagtagttgt
     2461 aggccagccg tgtctgcacc ggcgagacaa cccggatttt ccggctcacg gccggcagca
     2521 cggccggcag gttcagttga tcgccgttcc aggagacgtg gcagcggcag aactccttca
     2581 gataatgata gtaggccgat gcggcggcaa ctccgctgct gccgcgcagg acgatcctgc
     2641 ccggttcgcc cgactcgatc tcgtagacat ccttgccgtc cgccggcggg atgaactcga
     2701 cggcgaatct gtcggcgcat cccggcgtca ttcgttggat cagcgccttt acagcctccg
     2761 gtccggcggc ttcggccttc tccggcgcgg cggccgcggc agccgccagc gatatggata
     2821 tcatcaacag actcaaaagt tttatgaccg ttctcatgct gggttccctt tccgttcgaa
     2881 atgcgccgtg cggttttact acggaaggcc ttcgggcgca aaacggaaca cggaaaagat
     2941 cattcggccg tgccggtgag gtgccggccc ggcggctnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnccg gcggctggtt ccggaaaagc ttcgacaggg cgggcgttcc
     3121 gtggtttaac agcccgctta tgtacaaaaa aggtcagatg atcgagacgg agcttttcga
     3181 tacggcggac aagaaccgct gcgtcggcaa gctggaggat ggaatttgcg tgtttgtgga
     3241 aggcaccgcc gccgtcggcg ataccgttcg cgctgaaatt tataagacca aaaagcgcta
     3301 tctggtcgcc tcgctgaagg aaattctgaa gttttccgac cgccgcaccg cgccgcgctg
     3361 taagtatttc ggcctttgcg gcggctgcaa gtggcagcat ctcgactatt ccgagcagct
     3421 gcgcgtgaag cgcaaacagg tgctggacgc gctcgaacac ctcggcggct tcgaaggtcc
     3481 ggaggtggcg gactgcatcc cggcggaaga aattttcggg taccgcaaca agatcgacat
     3541 ggatttcacc gaccggcgct atctgaccgc cgatgagatg aatatcccgg aggccgagct
     3601 gagcaagcct ctcgatttcg cgctcggctt ccacgcgccg ggctgtccgt ttaaggcgat
     3661 cgatatcgac cattgcgacc tcgcctcgga agagatgaat ctggccgtcg aaaccgtccg
     3721 ccgcttctgc cgcgaccgca agctgtcgat ctattcgacg ttcacgcacg aaggcttcct
     3781 gcgcaacctc gtggtccggc gcggcgagcg caccggcgag ctgatggtga atctgattac
     3841 gctgacccac gatccggagc tgatgcgcga actctgcacc gcgttgcagg ccgcgctcgg
     3901 cgccaggctg acgacgttca tcaacgcgac gaccgaccgg aagaatatgg tggcgttcgg
     3961 cgatcaggaa tatgtgctgt gcggcgaagg cagcattagc gaccggctcg gcgaatacac
     4021 ctaccgcatt tcgcccaact cgtttttcca gaccaatacg gtgcaggccg aaaagctcta
     4081 taacgaaatt ctgaaggccg cgcggctgac cggcagcgaa acggtctacg accttttctg
     4141 cggaaccggt tccatcgcgc tctttgccgc gaaacactgc ggtaaggtgc tggggatcga
     4201 gctggtggag gccgccgtgg ccgacgcccg gaagaatgcg caggagcacg gcgcggcgaa
     4261 ctgctcgttc cggcagatgg atctgatgca tttcggcaaa atccgcgcgg agctggaagc
     4321 gttcggcctg ccggacgttg tcatcaccga tccgccgcgg gcgggcatgc accccaaagc
     4381 ggtggaaatg ctgcgcgagc tggcgccgcc ggtggtcatt tatgtcagct gcaacccggc
     4441 ttcgctggcg cgcgacggac agctgctgtg cgagggtgga ctctaccggc tcatcagtac
     4501 gcagccggtc gatctgttcc ctcagaccaa ccacgtcgaa agcgtcgccc gcttcgagcg
     4561 ggtcagctaa gcgccgctat ttcattttcg acttcagttt gattctttta ccgtcaaccg
     4621 tgaagttgcc gcggcccaga tgatgaatcg tccgcgggtt tttcacgtcc gggtcgatcc
     4681 acgctttgac gacttgcaga atgaacaggc agtacttatc caccatgccg gtgtcgatta
     4741 cccggcactc gagattggcg aagcattctt tgatgagcgg ggctccggcg gttgaagcgg
     4801 gcaggggcgt gagtccgaac tttttaaact tgtcggtttt cgctccggac gtattgccgc
     4861 agccggcaac cttttcagca atctccacgg agggaatgtt gatgacgcat tcgccggtgg
     4921 ctttcagcag gccgaaggaa tagctccggt cgctgatgac gcagccgatc agcggcggct
     4981 caaattccat catggtctgc caggacatgg tcatgatgtt ggcgcgcccg tcgcgggcgg
     5041 ttgtgaccag gacgaccggc cccggctcaa gcagtccgta gaccttggac agcggaaagg
     5101 attttctagc catgcggaac ctcctggctt tacagctgtt aagcgtcagc tccggaggta
     5161 tttaatgtcc tgagcgggag gaaagcaaac cgtttgaagc cggtagtccg gacggacaga
     5221 tccgcgatgt tcgcttggcg gcgaaaccgg acgcaaaatg cagtaaagcg ttgaaatcgg
     5281 gaactctccg ttgcattctg ctggcggttt tttccgccgg accggaaaaa cgtatgaagt
     5341 tcagcgccgg tttaaaaatt ttggtcagga aagcagagtt tcgggggttt ccgatggttt
     5401 ggaaaaaaga gtttcagttt gtggcgctgg cgcttctgct gagtgctgcg ggcggctatg
     5461 cggaaacgaa tcagacggct gctgctgatg ataaacatcc ggcagcctgc ccgcattgcg
     5521 gttttacgcc gaccaatccg gtggcggcgg ccggttcgac ggtgaccaat cgggcgtcga
     5581 cgaacgactg gaagtccagt gtgtacggcg ggttttccgc gaaaagcggt aatacggtgg
     5641 agcggtcgta caattacggg gcggatctgc agcgggtcgg caaaatttat cgcggcaccc
     5701 tgcaggcgga tgggcgctac agccggaccg aagatcaggt gacaacctca aagtccgaag
     5761 cctccggcga actccgccgg atgctggacg aacactggtt cgcctacggc gtgctttcag
     5821 cgacgcatga tgacctcaag gatctttcgt accgcgtcaa aaccggtccg ggtatcggct
     5881 attatttcgt cgactccaaa gagctgacgg cggatgtcag ctccggtccg ctctatgtac
     5941 atgaaaagac cgcggacggt gcgagcggtt atctggcgtg gcgctttgcg caggggttca
     6001 actggcagat caccggcacg ttccgcgtgt ggacgttgac cgaggcgttt gtggatacca
     6061 cggataccgc cgcttacacc atcgcggcca aagccggcgt cgaaagcaag atcagcggca
     6121 atttgagcct ggtcgtgatg atcgaagacg attatgacag cctgccggaa actcaggatg
     6181 agatcgaaaa aaacgacttt gaaatcagca ccgggcttcg ctattccttt tagcccgccg
     6241 gaaatgcacc atgactaaga aaagctacta cgtaacgaca ccgatctatt atgtgaacga
     6301 caagccgcac atcggccacg cgtacaccac cattctggcc gacgtgctcg ccagctatca
     6361 ccgccttctc ggcaccccga cctggttcct gaccggcacc gacgaacacg gccagaaggt
     6421 ccagcaggcc gccgataaaa acggactgac gccgcagcag cagtgcgatc agaccgttgt
     6481 ccgcttccag gagctgtgga agcgtctcga aatcaccaac gacgatttca tccgcaccac
     6541 cgaagagcgt cacaaagtca tcgttcagga agtgctgcag gatctctacg accgcgacga
     6601 aatctaccgc gcagaatacg aaggctggta ctgcgtcggc tgcgagcgct tcttcgccga
     6661 aaaagacctc gtcgacggca actgcccgga gtgcgggcgc aaagtcgacg ctatcgtcga
     6721 gaccaactac ttcttccgca tgagcaaata tcaggagtgg ctcatcgact acatcgaaac
     6781 ccatccggag ttcatccagc ccgacttccg cgccaacgaa accctcggct tcctcaaaac
     6841 caaaaagctg caggacctct gcatctcgcg gccgaaatcg cgcctcgcgt ggggcatcga
     6901 gctgccgttc gacaaggagt tcgtcaccta cgtctggttc gacgcgctgc tcaactacat
     6961 ctccgccatc ggctacaaac gcgacgacgc caaattcaac cagtggtggc ccgccacgca
     7021 ccagctcatc ggcaaagaca ttctcaccac gcacaccgtc tactggccga ccatgctcaa
     7081 agccatgaac gtgccgctgc cgaaaaccgt cttcgcccac ggctggtggc tcaccggccg
     7141 caccaaaatg agcaagagcc tcggcaacgt cgtcaacccc atggaaatgg tcgaccgcta
     7201 cggcgtcgat gccttccgct atttcctcat cgccgaaatg accctcggcc aggacgcctc
     7261 cttcaccgaa gaagccttca tccgccgcta caactccgac ctcgccaacg acctcggcaa
     7321 cctcatgagc cgcaccgtca acatgctggg ccgctactgc gacggaacgt ttccggcact
     7381 cggcagcggc gcgctcgacg gcgcgccgga gcaggaactt tggactgccg ttcagcaggc
     7441 ggttgtcgaa atggaaagct ccatcggcac aatgcacctc aacggcgggc tggccgcggt
     7501 gctggccgcc gtgcgcaagg tgaaccagta ttttgaaatc aaacagccgt ggacgcaggc
     7561 caaagaaggg aagaccgaag ccgtcggcgt aacgctggcg ctggccgccg aaagcctgcg
     7621 cgtctgcgcc gcactgctct atcccgtcat gccggaaaaa atgagcgcgc tgcgcgccgc
     7681 cttcggcctc ggcaagccgg aactcagcca gctgcgcacc tttggcgtca tcaaagccgg
     7741 aaccgtcatt ggcaatcccg gcatcctctt cccgcgcgt
//
LOCUS       DAOPZO010000089         7716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_11400_length_7716_cov_7.680068, whole genome shotgun sequence.
ACCESSION   DAOPZO010000089 DAOPZO010000000
VERSION     DAOPZO010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7716
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11400_length_7716_cov_7.680068"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..>2108
                     /locus_tag="PKI68_06655"
     CDS             19..>2108
                     /locus_tag="PKI68_06655"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNX53408.1"
                     /translation="MRIDNAQYQVEYQAAQGEFQILHKPSGRTFARHGRFENGCGTAA
                     VKSVSDKTFGKGQGIEVVYSNGCADVLALYPDLPFVLFTTVLQNGTAATQTVNRVSTF
                     SVGVESGTALEKVRTLGTGGLLAPGQNPGSYAFLSIVDPASRSGAVGGWLTHERGSGV
                     VFSPVQDGAVRMQARVDYGCLRLKPGAQTVTETFALGWFEDARLGLEAYADAVAKVYS
                     IKLLPQQAGHCTWYMEKNGRACNETGLVALAEVAQRELKPFGFDFILIDDYWQVGSRD
                     NGPAKIFTTHATNGPYPNGMKAAADGLVKSGLTPGIWFMPFAGNYKEEFFKAHLDWFV
                     KDQRGQPYDTAWGGTCLDMTHPDAQEYVRSIVQRITKQWGYTVLKMDGYWTGSATKQI
                     YVNAGYKEDGMGDATFFNPEKTNIEALRDGTKLVRAEAGTNVFLLGCCISQNMRSFGG
                     TFGLLDAMRVGPDTGGNIGSKHSSPVWFLNGRVWWNDPDCVMVRTKFPVERARLNASW
                     TAIAGQLFYNSDWIPDLTAERLDILKRCMPAHGLFSRPVDVFESDIANIWLLTDTRQA
                     VRRDVVALYNWGREEQEILCPAERVGLPAAPSYVAFDFWANRFVPPFSGSINARLPGA
                     SCRVLAVRPASPVPQLLSTSRHITQGMVDVSNEVWDASASALSGVSKVVANDAYELRL
                     VVPAGEKSWQAVEAR"
     assembly_gap    2109..2208
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2209..2829
                     /locus_tag="PKI68_06660"
     CDS             <2209..2829
                     /locus_tag="PKI68_06660"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HNX53409.1"
                     /translation="KVFTAVEPGLFDLPEKTATYRYTVTALGWTPEAKSAGVSADIRF
                     EAITAPPLPPAPTVHLSDLPGVKTANKKGQPKPNATINGSPLKIASKIYAKGIGVFAP
                     AQTICPVPAGATHFVAVVGVDAEAGAQATVVFKVYGDVKEMGEEPELLAESPILNTHT
                     LKSWSFNVELNTRFKELRLVVTESDDGNDCDYGDWVDAGFILKGAH"
     gene            2906..4525
                     /locus_tag="PKI68_06665"
     CDS             2906..4525
                     /locus_tag="PKI68_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNX53410.1"
                     /translation="MIKNNGWVKRAGIAGLVGAPLFGWGAAPQAAQPPVQSKDAPFNV
                     LFIAVDDLNDWTGRMGGNRQAITPNMDRFENQSILFANAHTPMAVCIASRNALLSGRH
                     PSTTGWYATGESAKAMQESYPSVMQGRSMLPAYFKKNGYKTYCAGKIFHGGATDFPFL
                     LNELWDDVLPSYDSQVTGELRERGGGYGGLIFYPFPKEGSQLKNHYGKELGGGHSLCG
                     GPLDPEDIPGGKMYDELIADYAVKELGKKQDKPFFLAVGFVRPHMPYTAPRKYFDMYD
                     KDKIQLPDVPDDEMKDIPVIGKAIAYGTAKMGDHQAVLGIPDYWRDMVHAYLACTTFV
                     DDQIGQVLRALDASPYASNTVVVLFSDHGQHLGEKKHWRKMSLWEESTRLPLAIRLPG
                     NRNNGQVCNRPVSLLDLYPTLVDVCSLPAVNGLEGRSLAPLLDQPAREWPYPVLSNWM
                     YKNYSVRSERYRYIQYRDGSEELYDHQVDPNEHINQANNPEFRDIIEQHKKWIPANPA
                     LPVGSTVWKGDHLTKQVELWKGKDGIPAWLE"
     gene            complement(4610..5626)
                     /locus_tag="PKI68_06670"
     CDS             complement(4610..5626)
                     /locus_tag="PKI68_06670"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HNX53411.1"
                     /translation="MKQTNEKAIKALLEKSGFRFSSLSDIPLKTVRHVQRGAFPDMRM
                     IELTRIQCEKFLQQPILQDLLCTRIGYQGPSPAHYIPRPDGSRDQILIFCTNGRGWLE
                     LDGKEWPVNRHDVFLIPRGKPHVYGADPDMPWSNYWVHFRGRQSPVFADLIGRAKSPV
                     VHLHRHEEIVASFEQLYHRMSNAHTVPELIAASGTLSQLLCMIQLRMGSAELKNQNAE
                     AAIERSLDFMHRNLEKKLRLRDLARVAGISPGHYGMLFHKKHRQTPIDYFNRLKIQKA
                     GELLKSTNSPISTIGESLGFLDPYYFSRLFKKVIGISPRAYRGTAVTTRYSATDRCVR
                     SENV"
     gene            5795..5989
                     /locus_tag="PKI68_06675"
     CDS             5795..5989
                     /locus_tag="PKI68_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53412.1"
                     /translation="MTDRNSGVIKQRADAASWYNGAFKLGTLLKKQIDRCCNNAEFLI
                     FRCFVKTEPVAGSAVGKAVS"
     gene            6038..>7716
                     /locus_tag="PKI68_06680"
     CDS             6038..>7716
                     /locus_tag="PKI68_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HNX53413.1"
                     /translation="MLNQLDFGILIGYVLLVITVGCGAAYFQKRKAARSGAAQDNGAY
                     FLAARTLTWPIIGLSLFSTNISTVHIVALCEEGFRSGLAYANFELAAVFTLVILAVFF
                     VPFYLRSHITTLPDFLEKRFNRNCRDFLAFISIISAIFIHIGVSLYAGAVVINAMMGL
                     STSVASLMPTMIMIAVATGLYVVVGGLLAVTLTDTIQTTTLLLGSAVVTWFAFNKLGG
                     WQPLYNTVGPNMISVLRPTGDFSGLPWHAVVLGYPVIGIWYWCTDQTIVQRVLGARNE
                     AHGKAGALFTGFLKLLPMFLFVLPGLLCLALVNNGLLPPLANTKDAFAHMVMNLLPAG
                     FRGLIVAGLLAALMGTIAGALNSIATLFAFDLYKRFKPETSDRKLVHIGRAATIVGVV
                     LAIIWSPIIGKFDSIYSAIASMICYISPPITAVFMTGIFWKRATAKAGAITLWTGFAM
                     GMIVFALDLFKAHTGWSMLFMHSAGMLCGICILIMIAVSLLDKDTNTEENLKLVWDSP
                     LTPLRIKGVKGLMNYKFLSIVVLLVASTIYFLFRAPTAEKIAAWKTANPEAA"
BASE COUNT         1566 a         2062 c         2275 g         1713 t
ORIGIN      
        1 tcgcctgcag cggcgagtgt gcgcattgat aacgctcagt atcaggtcga gtaccaggcc
       61 gctcagggcg agtttcagat tcttcataaa ccgtccggcc gaacctttgc ccggcacgga
      121 cgtttcgaga acggctgcgg aaccgccgca gtaaaatccg tcagcgataa aaccttcggg
      181 aaggggcagg gtatcgaggt cgtttactcc aacggctgcg ccgatgtgct ggcgctctat
      241 cccgacctgc cgtttgtgct gttcacgacg gtgctgcaga acggaaccgc ggccacgcag
      301 acggtcaatc gggtgtcgac tttttccgtc ggcgtcgaat ccggcacagc ccttgaaaag
      361 gttcggacgc tgggaaccgg cgggctgctg gctcccggtc agaatcccgg cagttatgcc
      421 tttttgtcta tcgtcgatcc ggcctcgcgc agcggcgccg tcggcggctg gctgacgcac
      481 gagcgcggca gcggcgtggt tttttcgccg gtgcaggacg gcgcggtccg gatgcaggcc
      541 cgcgtggatt acggctgttt gcggctcaag ccgggcgcgc agacggtgac ggaaacgttt
      601 gcgctgggct ggttcgagga tgcccgtctg gggctcgaag cctatgcgga tgcggtggcc
      661 aaggtctatt ccatcaaact gctgccgcag caggccggtc attgtacctg gtatatggag
      721 aagaacgggc gggcgtgcaa cgagaccggg ctggtggcgc tggccgaggt ggcgcagcgc
      781 gagctgaaac cgttcggctt tgattttatc ttgattgatg actactggca ggtgggctcg
      841 cgcgacaatg ggccggctaa aatcttcacc acccatgcga cgaacggacc ctatccgaac
      901 gggatgaagg ccgccgcgga cggtcttgtg aaaagcgggc tgactcccgg catctggttc
      961 atgcccttcg ctggcaatta caaagaagag tttttcaaag cccatctcga ctggttcgtc
     1021 aaagatcagc gcggccagcc gtatgacacg gcctggggcg gaacctgtct ggatatgacc
     1081 catcccgatg cgcaggagta tgtgcggagc attgtgcagc gcatcacgaa gcagtggggc
     1141 tacaccgtgc tgaagatgga cggctactgg accggcagcg cgaccaagca gatttatgtc
     1201 aatgccggct acaaagagga cggcatgggc gacgccacgt tctttaatcc cgagaagacc
     1261 aacatcgaag cgctgcgcga cggaaccaag ctggtgcgcg cggaggccgg aaccaatgtc
     1321 ttcctgctcg gctgctgcat cagccagaat atgcgaagct tcggcggcac cttcggtctg
     1381 ctggatgcca tgcgcgtcgg tcccgatacc ggcgggaaca tcggatcgaa gcattcctcg
     1441 ccggtctggt ttctgaacgg ccgggtctgg tggaacgatc ccgactgtgt gatggtccgc
     1501 acgaaatttc cggtcgaacg cgcccggctg aatgcctcct ggacggccat tgccggacag
     1561 ctgttttata acagcgactg gataccggat ctgacggcgg aacggctgga tattctcaag
     1621 cgctgcatgc cggcgcacgg actgttctcg cggccggtgg atgtgttcga gagcgacatc
     1681 gccaatatat ggctgctgac cgatacccgg caggcggttc gccgggatgt ggttgcgctg
     1741 tacaactggg gccgcgagga acaggaaatt ctctgcccgg ccgaacgggt cggcctgcct
     1801 gcagcgccga gctatgtggc gttcgatttc tgggccaacc gatttgttcc gcccttcagc
     1861 ggcagcatca acgcgcgtct gccgggggcc tcgtgccgcg tgctggcggt gcgtccggcg
     1921 tcgccggtgc cgcagctgct gagcacctcc cggcatatca cgcaggggat ggtcgatgtg
     1981 tcgaacgaag tctgggatgc gtcggcctcg gctctttcgg gggtcagcaa ggtggtggcc
     2041 aacgatgcgt atgagctgcg cctggtggtg ccggccggtg aaaaatcctg gcaggcggtg
     2101 gaggcccgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa agtgtttacc
     2221 gccgtggagc cggggctttt tgatctgcct gaaaagacgg cgacctaccg ctatacggtc
     2281 accgcgctcg gctggacccc ggaggcgaaa tccgcgggag tttcggcgga tatccggttc
     2341 gaagcgatca ccgctccgcc gctgccgccc gcaccgacgg tgcatctgag cgatctgccc
     2401 ggggttaaaa cggccaataa aaaagggcag ccgaagccga acgcgacgat caacggatcc
     2461 ccgctgaaga tcgcttcgaa gatctatgcc aaggggattg gcgttttcgc ccctgcgcag
     2521 acgatctgtc cggtgccggc gggtgcgacg cactttgtgg cggttgtcgg agtggatgca
     2581 gaggccggcg cgcaggccac ggtcgtcttt aaggtctatg gcgacgtgaa ggaaatgggc
     2641 gaggagccgg agctgctggc cgaatcgccg attctcaaca cccacacgct caagagctgg
     2701 tcctttaacg tcgaactcaa tacccgcttc aaagagctgc gcctggtggt gacggagtcc
     2761 gacgacggca atgattgcga ttatggcgat tgggtggacg ccggattcat tctcaagggc
     2821 gcacactgag acggagcggc gggatgatga gccgcctgtt gcgcagcggg cgcaggcgag
     2881 cggttgttag aaaaggagag aattgatgat caaaaataat ggatgggtga agagggcggg
     2941 gattgccgga ttggtcggcg ccccgctgtt cggatggggc gcagctccgc aggcggctca
     3001 gccgccggtg cagtcgaagg acgcaccttt caatgtactg tttatagcgg ttgatgacct
     3061 caacgactgg acggggcgga tgggcggaaa tcgccaggcg atcaccccca atatggaccg
     3121 ctttgagaat caaagcatcc tcttcgcaaa tgctcatacg ccgatggccg tctgcattgc
     3181 ctcgcgtaat gcgctgctca gcggccggca tccctcaacg accggctggt atgcgacagg
     3241 cgaaagcgca aaggcgatgc aggaatcgta tccgtctgtg atgcagggtc gttcgatgct
     3301 tccggcgtac ttcaaaaaga acggctataa aacctattgc gccggtaaga tttttcatgg
     3361 cggtgcaacc gatttcccgt ttctgctgaa cgagttgtgg gatgacgtat tgcccagcta
     3421 cgattctcag gtcaccggcg agttgcggga gcgcggcggc ggttacggcg ggcttatttt
     3481 ttatccgttt cctaaagaag gcagccagct gaagaaccat tacgggaaag aactcggcgg
     3541 cggccactcg ctttgcggcg gaccgctgga tccggaagat attcccggcg ggaaaatgta
     3601 tgacgagctc attgcggatt acgccgtgaa agagctgggg aaaaagcagg acaagccgtt
     3661 tttcctcgca gtcggcttcg tgcgtccgca tatgccgtac acagctccgc gcaaatattt
     3721 cgacatgtac gacaaggaca aaatccagct cccggatgtt ccggatgatg agatgaagga
     3781 tattccggtg atcgggaaag ctattgccta cggaactgca aagatgggcg accatcaggc
     3841 ggtgctcggt attccggact actggcgcga catggtgcac gcctatttgg cgtgtactac
     3901 tttcgtcgat gaccagatcg gtcaggttct gcgggcgctc gacgcaagcc cgtatgccag
     3961 caataccgtt gtcgtgctct tttcggatca cggccagcat ctcggcgaga aaaaacattg
     4021 gcgcaaaatg agtctttggg aggagtcaac ccgtctgccg ctggcgatcc gtcttccggg
     4081 aaaccggaac aacggtcagg tgtgcaaccg cccggtgagc ctgctggatc tctacccgac
     4141 gctggtggat gtatgttctc ttccggccgt taatggtctt gaagggcgca gtctggcgcc
     4201 tttgctggat cagccggccc gcgaatggcc gtatcctgtt ttgagcaact ggatgtataa
     4261 aaactattcc gtgcgcagcg agcgctatcg ctacattcaa taccgcgacg gcagcgaaga
     4321 gctttatgat catcaggttg atccgaatga gcacattaat caggccaata acccggaatt
     4381 ccgggacatc attgagcagc acaaaaagtg gattccggcc aatccggctc tgcctgtcgg
     4441 cagcaccgtc tggaagggcg accatcttac caagcaggtt gagctgtgga aaggcaagga
     4501 cggtattcca gcctggcttg agtagctgtt caacgtacgc accgcaacgg cggtgcgtac
     4561 gtttctgcac aggatcgatt ccagaagaca aagacgattg ctgaccgtat caaacgtttt
     4621 cggaccggac gcaacgatct gttgcagaat accgtgtggt aacggccgtc ccgcggtagg
     4681 ctcgcggaga aattccgatc acctttttaa aaagccgtga gaagtagtag gggtctaaaa
     4741 agccgagcga ctcgccaatc gtgctgatgg gactgttggt ggattttagc agctcgcccg
     4801 ctttctgtat tttcagccgg ttaaaataat ctatcggagt ttgccggtgc ttcttgtgaa
     4861 aaagcatccc gtaatgtccc ggactgattc cggctactct ggcgaggtca cgcagcctca
     4921 gctttttctc cagatttcta tgcataaaat cgaggcttct ttcgatggcg gcctccgcgt
     4981 tctgattttt cagctccgcg gagcccattc ggagctgaat catgcatagc agttggctga
     5041 gcgtaccgga cgcggcaatc agttccggga cggtgtgtgc attgctcata cggtgataga
     5101 gctgttcgaa ggatgctacg atctcttcat gccgatgcag atggacgacc ggtgatttgg
     5161 ctctgccgat caggtcggca aaaacggggc tctgcctgcc ccggaagtgc acccagtagt
     5221 tggaccatgg catatccggg tcggcaccgt aaacgtgcgg cttgcctcgc ggaatcaaaa
     5281 agacgtcgtg ccggttgacc ggccactctt tgccatccag ttccagccag ccccggccgt
     5341 tggtgcagaa aatcagaatc tggtcgcggc tcccgtcagg ccgcggaatg taatgtgcag
     5401 gggaggggcc ttgatagccg atacgggtac acagcagatc ctgcagaatg ggttgttgaa
     5461 gaaatttttc acactggatc cgggtcagtt cgatcatgcg catgtccggg aaagcgcctc
     5521 gctgtacatg gcgaactgtc ttcagcggga tgtcggacag cgatgaaaac cggaagccgg
     5581 atttttccag cagcgctttg atcgctttct cgttcgtctg tttcattccc ctccgtgtaa
     5641 tttcaagagc acatcgcact gcatggaacc gcaaattatc aaatcgtctt attgtccatt
     5701 tttttcatga gttcgtctat ggctattttc ggcaggcagg aacacaattt ccaattgcgt
     5761 aaaattcttg gcgaacaggt gtgtggatgc agcaatgact gacagaaact ccggcgtaat
     5821 taaacaacga gcagatgccg ccagctggta caatggtgca tttaagctcg gcaccctttt
     5881 gaaaaaacag atcgaccgat gctgcaataa tgcggaattt ttgatcttcc gctgcttcgt
     5941 aaagaccgaa ccggttgccg gttccgccgt cggcaaggcc gtatcttagg agaaactgcc
     6001 gtctgaaatt tcggaactaa actaatcgga gaaaaatatg cttaatcaac tggatttcgg
     6061 aattctgatc ggctacgtgc tgctggtcat tacagtcggg tgcggcgccg cgtactttca
     6121 aaagcgcaaa gccgcacggt caggcgcagc gcaggacaac ggggcctact ttctggcggc
     6181 ccgtaccttg acctggccga ttatcggctt gtcgcttttc tccaccaata tttcgaccgt
     6241 tcacattgtg gctctgtgcg aagaaggctt ccgaagcgga ctggcctatg ccaacttcga
     6301 gctggcggct gtttttacgc tggtgattct ggcggttttc tttgttccgt tctatctgcg
     6361 ttcacatatc accaccttgc ccgattttct tgaaaagcgg tttaatcgca attgccgcga
     6421 ctttctggcg tttatctcaa tcatttccgc aatctttatc catatcggcg tttcgctcta
     6481 tgccggagcc gtggtcatca atgccatgat gggactcagc acctcggttg cctccctgat
     6541 gcctacgatg atcatgatcg ctgtcgcaac cggactttat gtggtcgttg gaggattgct
     6601 ggccgtcacc ctgaccgata ctattcaaac gaccacgttg ctgctcggtt ctgccgttgt
     6661 cacctggttt gccttcaaca agctgggcgg gtggcagccc ttgtataata cggttggccc
     6721 gaacatgatc tctgtgctgc gtcctaccgg tgacttcagc ggactgccct ggcacgcggt
     6781 agtgcttgga tatcccgtca ttggaatctg gtactggtgt acagaccaga ccattgtcca
     6841 gcgcgtgctc ggggccagaa acgaagcgca tggcaaggcc ggagccttat ttacggggtt
     6901 ccttaagttg ctgcctatgt ttttatttgt tctgcccgga ctgctttgtc tggcgctggt
     6961 gaataacgga cttcttcctc cgctggcgaa tacaaaagac gcctttgccc acatggtaat
     7021 gaatctgctg ccggctggtt tccgggggct gattgtcgcc gggctgctgg cggctctgat
     7081 gggaaccatc gccggtgcac tcaactccat tgccacgctc tttgcgtttg acctctataa
     7141 acgcttcaaa cctgaaacct ccgacaggaa actggtacat atcggccgcg ccgccaccat
     7201 cgtcggagtt gttctggcca ttatatggtc tccaatcatc gggaaattcg actccatcta
     7261 cagcgcgatt gcatcaatga tctgttatat ctcgcctccg atcaccgccg tttttatgac
     7321 aggcatcttc tggaagaggg ccaccgccaa ggcgggggcg atcacactct ggaccggttt
     7381 tgccatggga atgattgttt tcgcgctgga tctgttcaaa gcacataccg gttggagcat
     7441 gctctttatg cactctgccg gtatgctgtg cggaatctgt attctgatca tgattgcggt
     7501 atcgctgctc gataaagata ccaacaccga agaaaatctg aaactggtat gggactcccc
     7561 gctgacgccg ctgcggatca aaggagtcaa aggactgatg aactataaat tcctgtcgat
     7621 cgttgtactg ctggttgcgt ccactattta cttcctgttc cgcgcgccga cagcggaaaa
     7681 gatcgccgcg tggaaaacgg ccaaccctga agcggc
//
LOCUS       DAOPZO010000090         7476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_11950_length_7476_cov_8.603557, whole genome shotgun sequence.
ACCESSION   DAOPZO010000090 DAOPZO010000000
VERSION     DAOPZO010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 7476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7476
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11950_length_7476_cov_8.603557"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="PKI68_06685"
     CDS             <1..1055
                     /locus_tag="PKI68_06685"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNX53414.1"
                     /translation="NSANLVDLKQPMVQYNTTFGYGRSEFNSAATSENTENPFFAIPG
                     FSAAIPFKEGKYAVGLATYVPFGRSVAWNDTGYFAQNNYSYAGSMSVVDFTPNFAMRI
                     TDSLSIGVGPDFYYGRVKQETIYNPFVPPFPALPPGTKSTLEADGTAVGWNCAATWKM
                     TEKQRLTATYRSPFRIKYEGDNKLSGPIDMKSDADADIEYPTIVGLAYGIELTDTLRA
                     ELDGEWLEFSQYKTLTINDSSFGSQSYPQDLKDTWTAGAGLAWNFKPQWTLRSGYMYL
                     KNPTPDKNYGTLGPDEDQGVISFGLGYEIEHHAIDVGYAYGLFDGRNISGSSASPNGK
                     YDYNVHLLSLSYGYKF"
     gene            1064..2197
                     /locus_tag="PKI68_06690"
     CDS             1064..2197
                     /locus_tag="PKI68_06690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNX53415.1"
                     /translation="MAKDRLLVIDDELGPRESLRFLFKDIYDVTCMDSVDKGVAELKK
                     NPPDTIIMDIKMPGKTGIQGLKEIRAIDQQVSIIMLTGFGSLETAQEAIRHGATDYLK
                     KPFDTDEIREVVAGYVRRTKINRKRIAATEHLEALTTQLQEQLNVKEKLASLGEESSE
                     FVHDLGNPLSVINGYVQILIKDIKDKKVQGKELNVNYLEQIEKSVSRCRDMLTLWRER
                     SKRADSSIKKIELGDLVSEVALNAQTLATAKSAQVNLTKGPADCLIEGDNVQIFRSIQ
                     NIVGNALEALPASGGGVINVCWRTDGRRALVEVEDNGGGFPPEKIADLQTKYYTTKGE
                     SGGMGLGLFITKNIVEAHGGSLILANNTAGTGALVTLAFPRLN"
     gene            complement(2269..4077)
                     /locus_tag="PKI68_06695"
     CDS             complement(2269..4077)
                     /locus_tag="PKI68_06695"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNX53416.1"
                     /translation="MTAPTKNKKLLNWVAEVQKMCTPDRVVWCDGSKKEYDSLMAEMV
                     ASGMAIKLNEAKRPNSFAFNSDPSDVARVENRTYIASVKQEDAGPTNNWIDPVELKKT
                     MTALYTGCMKGRTMYVIPFSMGPIGSPIAKIGIELTDSPYVVVNMHIMARVSTKVLDV
                     LGENGEFIPCLHSVGAPLEPGQKDSRWPCAPIEKKYIAHFPEENLIWSFGSGYGGNAL
                     LGKKCLALRIASNMARHEGWMAEHMLILRFTNPEGKKFHIAAAFPSACGKTNLAMLQS
                     TVPGWKVETIGDDIAWMKPGKDGRLYAINPEAGFFGVAPGTSAQSNPMALASCAKDTI
                     FTNVVVTSDLDVWWEDMGVECAGGTDWKNNPWKPGMKDAEGKKILGAHPNSRFTARAA
                     NCPVICEDWENPAGVPIDIFVFGGRRATTMPLVHEAFSFNHGVYMGATAASEATAAAL
                     DVKSAIRFDPFAMTPFIGYHAGDYMQHWFEMGDLLGDKAPRCFYVNWFRKDQNGKWLW
                     PGFGENSRVLKWMCERVEGKVGARETAIGLMPKDGDLTLDGLNIPAADWAELMKVDKD
                     LFKATVANAEEYLAKLGSKLPARMTEQLEALKVRLG"
     gene            4248..4991
                     /locus_tag="PKI68_06700"
     CDS             4248..4991
                     /locus_tag="PKI68_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX53417.1"
                     /translation="MNIKILSVGLFEVNCAIVWGPAQQALIIDPGQDAARIEAVLKSN
                     GLAVAAYLLTHGHADHLCALNELHTARPAPVYIHAEDYQWAFAPQNQIPPYYPVPGKP
                     AAEFIHPEQPSLQNPPRPAGTPPAEGNLKDPLFGRGGRSNATNGVGSRVWNIAGLCFQ
                     CLETPGHTRGGVCYYFEQEKAIFTGDTLFKGTCGRTDLPGGDGRILAQSLKKLAALPG
                     DVTVYPGHNESTTITYEKKTNFFMQQAAG"
     gene            5094..5963
                     /locus_tag="PKI68_06705"
     CDS             5094..5963
                     /locus_tag="PKI68_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HNX53418.1"
                     /translation="MTASIEIPGRIDLQTGHGGLRYAALRCNGAEAHVYLYGAHVLHY
                     QPANQAPVLWQSQLSLFEPGKPIRGGIPLCWPWFGPHPSDSTQPGHGIARLTEWKPGS
                     SKATESATEITLRFPGSPVVDAALELTVILSDTLTVTMTTRNGGSAAIPVSSAMHTYF
                     AVSDIHEVSVTGLENSRYIDMLLPGQPVLTQPGAITFTAQTERRYIGTEQTCTIVDPG
                     LNRRIVIEKAGSRSTVVWNPWIEKAAAMPDFGNEEYLETVCVETANCGPDTLTLAPGA
                     THAMTARIRAEAL"
     gene            complement(6027..6959)
                     /locus_tag="PKI68_06710"
     CDS             complement(6027..6959)
                     /locus_tag="PKI68_06710"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNX53419.1"
                     /translation="MKFGKKTDWLTRLVVYITLASLLGFFGRVFWVLDLFAHFRVQYF
                     QICLVLILVALWKRRNKLAAALAVVACFNYAFILPFYFGKTAAEQVPTVRAMLMNINA
                     SNGNTEQVLKAIQTAAPDLLLLEEVTTKWSEHLTVLDAKYPYRVAEVRDDCFGIMLLS
                     KYPLSHSSVTYIGTAGVPSIVTTVHLPQVEISVIGTHPLPPAGRTYSQHRNDQLGKLP
                     QIVLDQKKPVLLIGDLNTSPWSSYFTRLLRDSGLKNSMQGFGFQPSWPSNIGFLRIPI
                     DHVLHTPEIVVRNRAVGTDVGSDHLPVIVDFSCR"
     gene            complement(6968..>7476)
                     /locus_tag="PKI68_06715"
     CDS             complement(6968..>7476)
                     /locus_tag="PKI68_06715"
                     /inference="COORDINATES: protein motif:HMM:NF037213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HNX53420.1"
                     /translation="PMQRGELGARNLNQVMQGLLNPAGREIERFGMVFREGDRVMQTE
                     NDYDKEVFNGDLGRIVAIDEEQREVVVKMDDRRVKYEFRELDELVHAYAVTIHKSQGS
                     EYPCVIIPVHTQHFVMLQRSLLYTAVTRAKKFAVLVGTKKAMGLAVSRAESRERVTTL
                     KERLQSQK"
BASE COUNT         1716 a         2275 c         1967 g         1518 t
ORIGIN      
        1 acaactcagc caatctcgta gacctcaaac agccaatggt tcagtacaac accacattcg
       61 gttacgggcg ctcagaattc aacagcgcgg caacatcgga aaacaccgaa aatccgttct
      121 ttgcgattcc cgggttttcg gccgccattc cgttcaaaga aggcaaatat gcggttggac
      181 tcgccaccta tgttccattc ggccgctcgg ttgcatggaa tgacaccggc tattttgcac
      241 aaaataacta ctcgtacgcg gggagcatgt ccgtcgtaga ctttaccccg aactttgcca
      301 tgagaataac cgactccctt tcgatcggcg ttgggcccga tttttattac ggacgcgtaa
      361 aacaggaaac catttacaat ccgtttgtcc cgccatttcc cgcccttccc cccggaacaa
      421 aatcgacatt ggaagcagat ggaaccgccg tcggctggaa ctgcgcagca acctggaaaa
      481 tgactgaaaa acaacggctg acggccactt accgatctcc attcagaata aaatacgaag
      541 gcgacaataa actttccggg cctatcgaca tgaaaagtga tgctgatgcc gacatcgaat
      601 atccgaccat tgtcgggctg gcttacggaa tcgaactcac agacacctta cgcgccgaac
      661 tcgacggcga gtggcttgag ttttcccaat acaaaaccct aacgatcaat gactcatcat
      721 ttggatctca atcttaccct caagatttaa aagacacatg gacagctggt gccggcttgg
      781 catggaactt caagcctcaa tggacattac gttcgggata tatgtatctc aagaacccca
      841 ctccggataa gaattacggc acactcggac cggacgaaga ccagggggtt atctcgttcg
      901 gtctcggata cgaaatcgaa catcacgcca ttgatgtagg ctatgcctac ggtttgtttg
      961 acggacgtaa cattagcgga tcgtcagcaa gtccgaatgg gaaatacgac tataatgttc
     1021 acctgctcag cctctcttac ggttacaaat tctaggaaaa accatggcaa aagaccggtt
     1081 acttgtaatt gatgatgaac ttggcccgcg ggaaagtctc cgcttccttt tcaaggatat
     1141 ctacgatgtg acctgcatgg attctgtcga taaaggagtg gccgaactca aaaaaaatcc
     1201 gccggacacc atcatcatgg acatcaaaat gcccggcaaa accggcatac aaggcctgaa
     1261 agaaatccgc gccattgacc agcaggtctc catcatcatg cttaccggtt ttggatcgct
     1321 cgaaaccgca caggaagcca tccgccacgg cgcgaccgac tatctcaaaa aacctttcga
     1381 tacagacgaa atccgcgaag tcgtcgccgg ctatgtccgg cgcacgaaaa tcaaccgcaa
     1441 gcggatcgcc gcgacagagc atctcgaagc tttaacgacg cagctccaag agcagcttaa
     1501 cgtcaaggaa aagctggcgt ctctcggcga agaatcatcc gaattcgtcc acgatttagg
     1561 caacccccta agtgtcatta acggctacgt tcagatcctc atcaaagaca tcaaagacaa
     1621 aaaagtccaa ggcaaggaac tgaatgttaa ctatcttgag cagatcgaaa agagcgtctc
     1681 ccgctgccgc gacatgctca ccctctggag agaacgttcc aagagggcgg actcttccat
     1741 caaaaaaatc gagctcggcg atctcgtatc cgaagtagcc ctcaacgccc agactctcgc
     1801 caccgcaaaa tctgcgcagg tcaacctgac caaaggcccg gcggactgcc ttatcgaagg
     1861 cgacaatgta cagatcttcc gttccatcca gaacatcgtc ggcaacgcgc tcgaagccct
     1921 gcctgcctcc ggcggcggcg tcatcaacgt ctgctggcgg accgatggac gccgggcact
     1981 cgtcgaagtg gaagataacg gcgggggctt ccctccggaa aaaatcgccg acctgcaaac
     2041 caagtactac acaaccaaag gcgaatccgg cggcatgggc ctcggcctct tcatcacaaa
     2101 aaatatcgtt gaagcgcatg gtggatcgct gattctggcc aacaacacgg ccggaaccgg
     2161 ggcgctcgtc accctcgcct tcccgcgcct gaactgacag cgcccgcgaa aataaaaagg
     2221 cgctccggaa ttccggagcg cctttcgctt ttcagcggtc agggctggtt agccaagacg
     2281 gactttgaga gcttcgagct gttcggtcat gcgggccggc agcttcgaac cgagcttcgc
     2341 cagatattct tcggcgttgg caacggtggc tttgaagaga tctttgtcca ccttcatcag
     2401 ctccgcccag tcagcggccg gaatattcag gccgtcgagg gtcagatcgc cgtctttcgg
     2461 catcaggccg atggcggttt cgcgggcgcc gactttgcct tcgacgcgct cgcacatcca
     2521 tttgaggacg cggctgtttt cgccgaaacc gggccagagc catttgccgt tctgatcctt
     2581 gcggaaccag ttcacataga agcagcgcgg agctttatcg cccagcagat cgcccatttc
     2641 gaaccagtgc tgcatgtaat cgccggcgtg gtagccgatg aaaggggtca tcgcgaaggg
     2701 gtcgaaccgg atggccgatt tgacgtccag cgcggcagcg gtagcttcgg aggccgcggt
     2761 ggcgcccatg taaacgccgt ggttgaagct gaacgcttcg tgtacgagcg gcatcgtggt
     2821 cgcgcggcgt ccgccgaaca caaagatgtc gatcggaaca cccgccggat tttcccagtc
     2881 ttcgcagatg accgggcagt tggcggcgcg agccgtgaag cggctgttcg ggtgggcgcc
     2941 gaggattttc ttaccttcgg catccttcat gcccggcttc cacggattgt tcttccagtc
     3001 cgtaccgccg gcgcattcca cgcccatatc ttcccaccag acgtccaggt cggaagttac
     3061 gacgacgttg gtgaaaatgg tgtctttcgc gcagctggcc agcgccatcg ggttggactg
     3121 tgcgctggtt cccggcgcaa cgccgaagaa accggcttcc ggattgatcg catagagacg
     3181 tccgtctttg ccgggcttca tccacgcaat atcgtcgccg atggtttcga ccttccagcc
     3241 cggaacggtg gactgaagca tcgccagatt ggttttgccg caggccgacg gaaacgcggc
     3301 cgcgatgtgg aattttttac cttccggatt ggtaaagcgc aggatgagca tgtgctcggc
     3361 catccagcct tcatgacggg ccatgttcga ggcgatgcgc agggccagac attttttgcc
     3421 gagcagtgcg tttccgccgt aaccggaacc gaaggaccag atcaggtttt cttcagggaa
     3481 gtgcgcgatg tatttctttt caatcggcgc gcagggccag cggctgtcct tctgaccggg
     3541 ctcaagcgga gcgccgaccg agtgcaggca cgggatgaat tcgccgtttt cgccgaggac
     3601 gtcgagcacc ttggtgctga cgcgggccat gatgtgcata ttgaccacca cgtacgggct
     3661 gtcggtcagt tcaataccga tcttagcgat cggcgagccg atcgggccca ttgagaacgg
     3721 gatgacatac atcgtgcggc ccttcatgca gccggtatac agagccgtca ttgtcttctt
     3781 gagttcaacc gggtcaatcc agttgttggt cgggccggca tcttcctgct tcaccgaagc
     3841 aatgtaggtg cggttttcaa cgcgcgccac gtcggacgga tcggagttga aagcgaagct
     3901 gtttgggcgc ttggcttcgt tcagcttgat cgccataccg ctggccacca tttcggccat
     3961 caggctgtcg tattctttct tcgagccgtc acaccagacc acgcggtcgg gcgtacacat
     4021 cttctggact tcagccaccc agttgagcag cttcttattc ttcgtcggtg cagtcattat
     4081 gcaatcctcc attaagttac ctatcatcaa attcggttaa aattctgcat aaggtgcgga
     4141 aatgatctcg gcaagtcaac cactgaaccc ataaaattgc ggactggcac tgacattttc
     4201 acgattcccg ctttcacgcc agccccgcgt tacctattct cccccgcatg aatataaaaa
     4261 tcctttctgt cggcctcttt gaagtgaact gcgccatcgt ctggggcccg gcccaacagg
     4321 ctctgatcat cgacccggga caggacgccg cccggatcga agccgtcctg aaaagcaacg
     4381 gcctcgccgt tgccgcatac cttttaacgc acggtcacgc cgaccacctc tgcgccctca
     4441 acgaactgca caccgcccgc ccggcaccgg tctacatcca cgccgaagat tatcagtggg
     4501 ccttcgctcc gcagaaccag atcccgccct actatcccgt gcccggcaaa cccgccgccg
     4561 aattcatcca cccggagcaa ccctcactac agaacccacc ccgccctgcg ggcacccctc
     4621 cggcggaggg gaatttaaaa gatcccctct ttgggagggg tggacggagc aacgcgacga
     4681 acggggtggg ttccagggtt tggaacatcg ccggtctttg cttccagtgc ctggaaacgc
     4741 ccggccacac ccggggcggc gtctgctact actttgagca ggaaaaagcc atattcaccg
     4801 gcgacaccct cttcaaagga acctgcggac ggaccgacct gcccggcggc gacggacgca
     4861 tccttgcgca gtcgctcaaa aagctggccg ccctgcccgg cgacgtcacc gtctatcccg
     4921 gccacaacga aagcaccacc atcacttatg agaaaaaaac caacttcttc atgcagcagg
     4981 ccgccgggtg aaaagccccc ggatccatct gttttaacta gcgtccggaa aggcgttccg
     5041 atagataaag acgctcgtat tgacgattcg aaaaatttta ctggagactt tttatgaccg
     5101 catcaatcga aattcccggc cgcatcgacc ttcagaccgg acacggcgga ttgcgctatg
     5161 ccgcactgcg ctgcaacggc gccgaagcac acgtctatct ttacggcgcc catgtcctgc
     5221 actatcagcc ggccaaccag gcaccggtgc tgtggcagag ccaactgagc ctattcgagc
     5281 ccggcaaacc gattcgcggc ggcatcccgc tctgctggcc gtggttcggc ccgcacccgt
     5341 ccgactcaac gcagccggga cacggcatcg cgcgcctgac cgaatggaag ccgggctcct
     5401 ccaaagcgac agagtccgca accgaaatca ccttgcgctt ccccggatcg ccggtcgttg
     5461 acgccgcgct cgaactgacc gtgattcttt cggacaccct gaccgtcacc atgacgaccc
     5521 gcaacggcgg cagcgccgcg ataccggtca gcagcgccat gcacacctac tttgccgtca
     5581 gcgacatcca tgaggtttcc gtaaccggcc tcgaaaacag ccggtacatc gacatgctgc
     5641 tgcccggcca gccggttctg acccagcccg gcgcgatcac atttacggca cagactgaac
     5701 gccgttatat cggcaccgaa cagacctgca cgatcgtcga tcccggactg aaccggcgca
     5761 tcgtcatcga aaaagccggc agccgctcga ccgttgtctg gaatccgtgg atcgagaaag
     5821 ccgcagccat gccggacttc ggcaacgagg aatatctgga aaccgtctgc gtggaaaccg
     5881 ccaactgcgg cccggacacc cttacactgg cgccgggcgc aacccacgcc atgacggcgc
     5941 ggatccgcgc ggaagcgctg taaagccgcc gcaacccccg gacggttctc tgaaccgtcc
     6001 ggcacggcac ctattttcag aactcctcat ctgcaactaa agtccactat gaccggcaga
     6061 tgatctgaac ctacatctgt ccccacggca cggttccgga ccacaatttc aggcgtatgg
     6121 agtacatgat cgatggggat gcggagaaag ccgatgttgc tcggccagct cggctgaaag
     6181 ccgaagccct gcatactgtt ctttaagccg gagtcgcgca gaagccgggt gaagtacgat
     6241 gaccatggcg atgtattcag atcgccaatg agcagaaccg gctttttctg atcgagaaca
     6301 atctgcggca gttttccgag ctgatcattc cgatgctgag aataggttct gcctgcaggc
     6361 ggcaaaggat gagttccgat gacagaaatc tcaacttgcg gcagatgaac cgttgtgacg
     6421 attgaaggaa ctccagcggt tccaatatag gtcacactgc tgtgcgaaag cgggtatttg
     6481 ctcagcagca taattccgaa acagtcgtcc cggacttcgg caacacggta gggatatttg
     6541 gcatcgagaa ccgtcagatg ctcagaccat ttcgtggtca cctcttccag cagcagcaag
     6601 tccggcgcag ccgtctgaat ggccttcagc acctgttccg tattgccgtt cgacgcatta
     6661 atgttcatca gcatggcgcg caccgtcgga acctgttcgg cggcggtttt gccgaaataa
     6721 aacggcagga taaaggcata gttaaagcag gcgacaacgg ccaaagccgc cgccagtttg
     6781 ttgcgccgct tccatagagc aacaaggatc agcacgaggc aaatttgaaa atactgcacc
     6841 cgaaagtgcg cgaataaatc cagcacccag aaaacccgac cgaaaaatcc cagcagtgac
     6901 gccaacgtaa tgtaaacaac cagtcgggtc agccagtctg tcttcttacc gaacttcatc
     6961 ctgtttccta tttctgactc tgaagccgtt ccttcagcgt cgtgacgcgt tcgcgggatt
     7021 cggcgcggct gacggccagc cccatcgcct ttttcgtgcc gaccagcacg gcgaacttct
     7081 tggcccgcgt aaccgccgtg tagagcaggc tgcgctgcag catgacgaag tgctgggtgt
     7141 ggaccggaat aataacgcag ggatattcgc tgccctgtga tttatgaatg gtgacggcgt
     7201 aagcgtgtac cagctcatcg agctcgcgga actcgtactt cacgcggcgg tcatccatct
     7261 tcaccaccac ttcgcgctgc tcttcgtcaa tcgccacgat gcgcccgagg tcgccgttga
     7321 aaacctcttt gtcgtaatcg ttctcggtct gcatcacgcg gtcgccttcg cggaacacca
     7381 tgccgaagcg ttcaatttcg cggccggccg gattcagcag gccctgcatc acctgattga
     7441 gattgcgcgc gcccagctcg ccgcgctgca tcggcg
//
LOCUS       DAOPZO010000091         7386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_12175_length_7386_cov_7.764016, whole genome shotgun sequence.
ACCESSION   DAOPZO010000091 DAOPZO010000000
VERSION     DAOPZO010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12175_length_7386_cov_7.764016"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PKI68_06720"
     CDS             complement(<1..338)
                     /locus_tag="PKI68_06720"
                     /inference="COORDINATES: protein motif:HMM:NF039198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HNX53421.1"
                     /translation="MSWHILLIGLLIFCARIVDVSIGTLRTIVTVQGRTRLAFALGFM
                     EVTLWVTIISAIVKQIHTAPVLALFYGLGFATGNVVGIALERKLALGHVALRAFTRKS
                     GGAIARQLRAA"
     gene            complement(462..710)
                     /locus_tag="PKI68_06725"
     CDS             complement(462..710)
                     /locus_tag="PKI68_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNX53422.1"
                     /translation="MNDAEILEAVEKIMDEEINPALAGHGGGAKVATVKDAKVYIELQ
                     GGCRGCMGARMTMKNGIERLLKEKIPAILEVIDATNHG"
     gene            complement(785..1522)
                     /locus_tag="PKI68_06730"
     CDS             complement(785..1522)
                     /locus_tag="PKI68_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX53423.1"
                     /translation="MATIAQYKEVKKVAVLMPAFCEERTVGTAVAKALKYLPDVIVVD
                     DASTDETVACAEAAGAAVIRRRVNGGKSAALTEGFKYALAYGFDAVIALDADGFHDPD
                     EIPRFLEAYQRTHLPVLIGNRMAELHRIPVVRRWTIRLMRYWLDRLLGVYVPDPPCGF
                     RFYRCDVLPFILEERSSLPSEFEMLLNIASRRIRVGSVRITKQPKRHKSPIAPFRDLI
                     RFFRVLWKYHKKTRHPQKPVRLVEEEL"
     gene            complement(1522..2391)
                     /gene="lipA"
                     /locus_tag="PKI68_06735"
     CDS             complement(1522..2391)
                     /gene="lipA"
                     /locus_tag="PKI68_06735"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNX53424.1"
                     /translation="MSELKPKAQRLPQWMRRPIATDEQYPDVQKLLASLQLNTVCASA
                     KCPNRHECWNRGTATVMILGNTCTRNCRFCNVNTGRPGPVDYDEPARVAEAARRLNLR
                     HIVITSVTRDDLPDGGAGVFATTIRAVKEAMPEASVEVLTPDFVMHLDTVLDAGPDVF
                     NHNLETVKRLQAAIRPQASYAKSLATLRKAAERGGVQVKSGLMLGLGETDEEIFECLN
                     DLYAEGVRLLTLGQYLAPTREHYPVERFISPEHFDELAARAGAIGFEGVAAGPLVRSS
                     YRADELARNRDNG"
     gene            complement(2401..5073)
                     /locus_tag="PKI68_06740"
     CDS             complement(2401..5073)
                     /locus_tag="PKI68_06740"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNX53425.1"
                     /translation="MAELPKNYEPAGVEAKWYAKWMEEKAFHAVPDAAKEPWTIVIPP
                     PNVTGILHMGHALNNTIQDVLIRQKRMQGKNALWVPGTDHAGIATQNVVERKLAKEGK
                     SRHDFSREEFLEHVWAWKEEYGNTITGQLKKLGSSCDWERERFTMDEGLSKAVLEVFC
                     RLYDKGLIYRGNRIINWCPRCTTALSDEESEHVETEGALYYIRYAVKDEKRKITVATT
                     RPETMLGDVAVAVSPRDKRYSDLVGKTLILPVLGREIPVVADEFVDPAFGTGCVKVTP
                     AHDPNDFEIGLRHKLAPINVMDDHGLMNEAAGPYAGMDRFECRKQLVEDLKHGGLIEK
                     IDKHMHAVGHCYRCATVVEPRLSPQWFVNMKPLAKPAIEAVRDGRIKFVPERWNKVYL
                     DWMENIRDWCISRQIWWGHRIPVFYCDACGHEWAAREVPGSCPVCGVKDIRQDEDVLD
                     TWFSSWLWPFSVFGWPEQTADLKHFYPTKSLVTASEIIFFWVARMIMAGCEFMGEIPF
                     DTVYIHGTVRDDQGRKMSKSLGNSIDPLNIIEKYSADALRFSLMMLTATGQDVYINDE
                     KFELGRNFCTKIWNAARFMQMNASGAAIDPDKIDFSRMKLSPDDQHILAKLGEAVRST
                     EDNLARHRFNDASLAMYDFMWHHYCDWYVEYSKSVFRGEDAQRKQEVELVMHYVFSNA
                     IRLLHPFIPFITEELWHGMGYNTSSESIQTAPWPKALSYEELSAWGVQRGTVEYVDAK
                     HDLIRVGRTLRTDYNLTPKQQAKFYVRPAQERSAKLLRDDIVSVSLLLGAESIGIDRD
                     FTPEGAMPSGISQLGTVYMSIEGLVDTDAEIKKLQKQLETVENGIMGITKKLSNENFV
                     SRAPAEVVAGEEKRKAELLEKREKLQKLIETLSV"
     gene            complement(5138..5527)
                     /locus_tag="PKI68_06745"
     CDS             complement(5138..5527)
                     /locus_tag="PKI68_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNX53426.1"
                     /translation="MKDLNTISHDIIQSAIEVHKELGPGLLESVYRKCLSKALRDTGC
                     QVEEEVYLPVKFRGEVVEENGYRIDLLVEKSVIVEVKSVETVKNLFSKQLGTYLKLTG
                     MHLGLLINFNEVMLRDGIKRIVNNFPE"
     assembly_gap    5636..5735
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5736..7313)
                     /gene="cimA"
                     /locus_tag="PKI68_06750"
     CDS             complement(<5736..7313)
                     /gene="cimA"
                     /locus_tag="PKI68_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HNX53427.1"
                     /translation="MSKKNIAIYDTTLRDGAQAEGVSFSAVAKVQVARRLDEFGVAYI
                     EGGFAASNPKDMEFFRLIKKESLKNAKVAAFGSTRRANIKPEEDKGLAALLEADTPVC
                     TIFGKSWLLHVTEVLQTTPEENLAMIADSVRFLKARGKEVIYDAEHFFDGYKDSPEYA
                     MAALKAAADAGADCLVLCETNGGALPHEVAAITAAVIKAFAAPVGIHTHNDGELGVAN
                     ALAAVQAGAVHVQGTINGIGERVGNCNLSSVIPNLMLKMGCNCLTKAENLKQLRALSL
                     SVYEQANIRPYTKQAFVGDSAFAHKAGMHVDGVRKVSRSFEHIVPESVGNSRRILISE
                     LSGASNVFLKAIEMGLELDRKSPEVREVLNQLEQMEKDGYEYESADASFQMLIKKVLK
                     QHQSFFDLKSFSVTVDKQGKGPGCRSEATVKLSVKGESVSANGTGDGPVDALNHALRD
                     ALVKFYPAIAGVSLIDYSVRILDPEEATAAKTRVLIESSDGVKTWGTVGVSENIIEAS
                     WEALVDSVEYKLFLEEEK"
BASE COUNT         1524 a         2204 c         1880 g         1678 t
ORIGIN      
        1 gcggcgcgca gctgccgggc aatcgcgccg ccggatttac gggtgaacgc gcgcaaagca
       61 acatggccca gcgcgagttt ccgctccagc gcaatgccga cgacgtttcc ggtggcaaag
      121 ccaagtccgt aaaacagcgc cagcaccggc gcggtgtgga tctgttttac gatggcggaa
      181 atgatcgtca cccagagagt gacttccata aagccgagcg cgaacgccag ccgcgtccgc
      241 ccctgcacgg tgacaatcgt ccggagcgta ccgatggaaa cgtccacgat acgggcgcag
      301 aaaatcaaaa gcccgatcag taagatgtgc cagctcaaat acaggctccg ttaaagtgct
      361 aaaaaaccga aactgttgct gtcgccattt aagcggggca gacattcctg tctgcccgtc
      421 ctgcgcagac aggaatgtct gcgccacaaa cagaacattc ttcagccgtg attggtggcg
      481 tcgataactt ccagaatcgc cggaattttt tctttgagaa ggcgttcgat tccgtttttc
      541 atcgtcatcc gtgcgcccat gcagccgcgg catccgccct gcagttcgat gtaaaccttg
      601 gcatctttaa ccgtggcaac cttcgcgccg ccgccgtgac cggccagcgc cggattgatt
      661 tcttcatcca tgattttctc tacggcttcg agtatttcag catcattcat gattcatttc
      721 ctctctttga ataaaacgta atccattgcc cggcggttgt caaaagatgc cgccgccccg
      781 gctctcacag ctcttcctcg accagccgga ccggtttctg cgggtggcgg gtctttttat
      841 ggtatttcca gagcacgcgg aaaaaccgga tcaaatcccg gaacggagca atcgggcttt
      901 tatgccgctt gggctgcttg gtgatccgga ctgaaccgac gcggatgcgg cgggaagcaa
      961 tgttgagcag catttcaaac tcggacggaa gcgacgaacg ctcttcgaga ataaacggaa
     1021 gcacgtcaca gcggtagaag cggaaaccgc acggcggatc gggcacatac acgcccagca
     1081 gccggtccag ccagtaacgc atcaggcgga tcgtccagcg gcgtaccacc ggaatacggt
     1141 gcagttccgc catacggtta ccgatgagca ccggcagatg agtgcgctga tacgcctcca
     1201 gaaaccgggg aatttcatcc ggatcatgga acccgtcggc gtccagtgcg atcaccgcat
     1261 cgaatccgta agccagcgcg tatttaaacc cttcggtcag cgcggcgctt tttccgccgt
     1321 tcactctgcg ccggatcact gcagcgccgg cggcttcagc gcaggctaca gtttcatcag
     1381 tcgaagcatc gtccaccacg atcacatccg gcagatattt aagcgccttt gcaacggcag
     1441 ttccgaccgt ccgttcttcg cagaacgcgg gcatcagaac cgcaaccttt ttcacttcct
     1501 tatactgtgc aatggtcgcc atcagccgtt atccctgttg cgggccagct cgtcggcgcg
     1561 gtaggaagag cgcaccagcg gtcccgccgc cactccttca aacccgattg ccccggcccg
     1621 tgcggccagt tcatcaaaat gttcagggga aataaagcgt tcaaccggat aatgttcccg
     1681 cgtcggggcc agatactgtc ccagcgtcag caggcggacg ccctccgcat acaaatcgtt
     1741 caggcattcg aaaatctctt catccgtttc gccgagtcca agcatcagac cggatttcac
     1801 ctgcacaccg ccccgctccg ccgctttacg cagtgtggcg agcgactttg catagcttgc
     1861 ctgaggccgg atcgccgcct gcaggcgctt cacagtttcc agattgtgat taaacacatc
     1921 cggcccggca tccagcacgg tatcaagatg cataacaaag tcaggcgtca gcacttcaac
     1981 agaggcttcc ggcatcgctt ccttcaccgc acggatggtc gttgcaaaaa ctccggcgcc
     2041 gccgtccggc agatcgtcgc gcgttacgct ggtaatcaca atatgccgca ggttcagccg
     2101 ccgggccgct tcggccacgc gcgccggttc gtcataatca accggaccgg gccggccggt
     2161 attcacattg cagaagcggc agttccgtgt gcaggtattg cccagaatca tcaccgtggc
     2221 tgttccgcgg ttccagcatt cgtggcggtt cggacatttc gcgctggcgc aaactgtatt
     2281 cagctgaagg ctcgccagca gcttctgcac gtcgggatac tgttcatccg ttgcaatcgg
     2341 ccgccgcatc cactgcggca gccgctgtgc tttgggtttc agttcactca ttccaaaacc
     2401 tcataccgag agcgtttcga tcagcttctg gagtttttcg cgtttttcga gcagttcggc
     2461 tttccgcttt tcctctccgg caaccacttc ggcgggtgcc cggctgacga agttttcgtt
     2521 ggaaagtttt ttagtgattc ccatgatgcc gttctcgacg gtttcgagct gcttctggag
     2581 cttcttgatt tcggcgtccg tgtccaccag accttcgatg gacatataaa ccgtgccgag
     2641 ctgactgatg ccgctgggca tcgcgccttc cggcgtgaag tcgcggtcaa tcccgatgga
     2701 ttccgcgccg agcagcaagg aaacggatac gatatcgtca cgaagcagtt tggcggaacg
     2761 ctcctgcgcc gggcggacat aaaacttcgc ctgctgtttc ggcgtcagat tataatccgt
     2821 gcgcagcgtg cgtccgacgc ggatcaggtc gtgcttggca tccacatatt caacggtgcc
     2881 ccgctgcacg ccccacgcgc tcaactcttc atacgacaga gccttcggcc acggcgcagt
     2941 ctgaatggat tcgctggatg tgttatatcc catgccgtgc cacagctctt cggtgatgaa
     3001 cggaatgaac gggtgcagca gccggatggc attggagaag acatagtgca tcaccagttc
     3061 gacttcctgt ttgcgctgcg cgtcttcgcc gcggaatacg ctcttggaat attcgacgta
     3121 ccagtcgcaa tagtgatgcc acatgaagtc atacatcgcc agcgaagcgt cattgaaccg
     3181 gtgacgggcc agattatctt cggtgctgcg aaccgcttcg cccagtttgg cgagaatatg
     3241 ctgatcgtcc gggctgagtt tcatcctgct gaaatcaatc ttgtccggat caatcgccgc
     3301 gccggaagca ttcatctgca tgaagcgcgc ggcgttccag atcttggtgc agaagttgcg
     3361 gcccagctca aacttttcgt cgttgatgta cacgtcctgc cccgtcgcgg tcagcatcat
     3421 gaggctgaag cgcagcgcgt cggcgctgta cttctcaatg atgttgagcg ggtcgatgga
     3481 gttgccgaga ctcttgctca tcttgcggcc ctgatcgtcg cgcacggtgc cgtgaatata
     3541 aaccgtatcg aacggtatct cgcccatgaa ttcacagccc gccataatca tgcgcgccac
     3601 ccagaagaaa ataatttccg aagccgtcac cagcgatttg gtcggataga aatgtttcag
     3661 atccgctgtc tgctccggcc agccgaatac gctgaacggc cagagccacg acgagaacca
     3721 ggtatccaga acgtcttcgt cctgccggat atctttcacg ccgcagaccg ggcagcttcc
     3781 gggaacttca cgggccgccc attcatggcc gcaggcatca cagtagaaaa ccggaatgcg
     3841 gtggccccac cagatctggc gggagatgca ccagtcgcgg atgttttcca tccagtcgag
     3901 gtaaaccttg ttccagcgct ccggcacaaa tttaatgcgt ccgtcgcgca cggcttcgat
     3961 ggccggtttc gccagcggct tcatattgac gaaccactgc ggcgaaaggc gcggctcaac
     4021 gaccgttgcg caacggtagc agtgaccgac cgcgtgcatg tgcttgtcga ttttttcgat
     4081 cagcccgccg tgcttcaggt cttcaaccag ctgcttgcgg cattcaaagc gatccatccc
     4141 ggcatacggt ccggccgcct cattcatcaa gccgtgatcg tccatcacat tgatcggcgc
     4201 cagcttatgg cgcaggccga tctcaaagtc gttcggatcg tgcgccggcg tcactttaac
     4261 acatccggta ccaaacgccg gatcgacaaa ttcatcggcc acgaccggaa tttcccgtcc
     4321 aagaaccgga agaatcagcg tctttccgac cagatcgctg tagcgtttat cgcgagggct
     4381 gacggccaca gcgacgtcgc cgagcatcgt ttccggacgc gtggtggcaa cggtgatttt
     4441 acgtttttcg tctttgacag cgtaccggat gtaataaagc gcgccctcgg tttcaacgtg
     4501 ttcgctctct tcatccgaaa gcgctgtcgt acagcgcgga caccagttga tgatacggtt
     4561 tccgcggtaa atcaaaccct tatcgtacag gcggcagaag acttcaagaa ccgccttgct
     4621 cagtccttca tccatcgtga aacgctcgcg ttcccagtcg caggaagaac caagcttctt
     4681 gagctgaccc gtgatcgtat tgccgtattc ttccttccag gcccagacat gctccagaaa
     4741 ttcttcccgt gaaaaatcgt ggcggctctt gccttctttg gccagcttac gctcgacaac
     4801 gttctgcgtc gcaataccgg cgtggtcggt gccgggaacc caaagggcat tcttgccctg
     4861 catgcgtttc tggcggatca aaacgtcctg aatggtgtta ttcagcgcat ggcccatatg
     4921 cagaataccg gtcacgttcg gcggcggaat gacaattgtc cacggctctt tcgccgcatc
     4981 gggtacggca tggaaggctt tctcttccat ccactttgca taccacttcg cttcaacccc
     5041 ggcgggttca taatttttcg gtaattcagc cataaatttg tcctgtcatt tataaatcaa
     5101 ctcgccgttc ctcgcagaga tgcagaggcc gcagagacta ttcgggaaaa ttattcacaa
     5161 tccgtttaat tccatccctc aacatcactt cgttgaaatt gattaaaagc cccaaatgca
     5221 ttccggttaa cttcaaatag gttcccaact gcttagaaaa aaggttcttc acggtttcga
     5281 ccgatttaac ctctacaatc accgattttt ccacgagcag atcaattcga tatccattct
     5341 cttcgacaac ttcacctctg aattttacgg gaagatacac ttcttcttca acctgacatc
     5401 ccgtatcgcg caaggccttc gaaaggcatt tccggtatac cgactccagc agtcccggcc
     5461 ccagctcttt atgaacctct atcgcagatt gaataatgtc atgcgatata gtgtttaaat
     5521 ctttcataaa tcctctgcga cctctgcatc tctgcgaggg atccgcctct atttccgacc
     5581 tttctcttct tcaagaaaca gcttgtattc cacgctgtcg accagcgcct cccacnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntttct cttcttcaag aaacagcttg
     5761 tattccacgc tgtcgaccag cgcctcccac gatgcttcga taatgttttc cgatacgccg
     5821 accgtgcccc acgttttcac gccgtcgctt gattcaatca gcacgcgggt tttagccgcc
     5881 gtcgcttctt caggatcgag aatgcgcacg gaatagtcga tcagcgacac cccggcgatc
     5941 gcgggataaa atttaaccag cgcatcacgc agggcgtggt tgagcgcatc gaccggaccg
     6001 tcgcctgtac cgttcgccga aacgctttca cctttaacac tcagcttaac agttgcctcg
     6061 gaacggcagc ctggcccttt cccctgctta tcgaccgtaa cgctgaaact cttcaagtca
     6121 aagaaggact gatgctgttt gagaaccttt ttgatcagca tctggaacga cgcatcggcc
     6181 gactcgtatt cgtagccgtc cttctccatc tgttcgagct gattaagtac ttcgcggacc
     6241 tcgggcgatt tccggtcaag ttccagcccc atctcgatcg ccttcaggaa aacgttgctt
     6301 gcgcccgaca gttcggaaat cagaatacgg cggctgttgc cgaccgattc cggaacgata
     6361 tgctcaaaac tgcgcgatac cttgcgtacg ccgtccacat gcattcctgc cttatgggca
     6421 aatgcgctgt cgccgacaaa cgcctgcttg gtgtaaggac gtatattcgc ctgctcgtaa
     6481 acactcagcg agagcgcgcg gagctgcttc agattttccg ccttcgtcag gcagttgcac
     6541 cccattttca gcatcagatt cggaataacg gaactcaggt tgcagttgcc gacccgttcg
     6601 ccgattccgt taattgtgcc ctgcacatgt accgctccgg cctgaaccgc cgccagcgcg
     6661 ttcgccacac ccagctcgcc gtcattgtgc gtgtggatgc cgaccggcgc agcaaaggct
     6721 ttgatcaccg cagcggtaat cgccgccact tcgtgcggaa gcgcgccgcc gttggtttcg
     6781 caaaggacaa ggcagtcagc cccggcatcg gcggcagcct taagcgcggc catcgcgtat
     6841 tccgggctgt ccttgtagcc gtcgaagaaa tgctcggcgt cataaatgac ttctttgccg
     6901 cgggctttca gaaaccggac gctgtcggcg atcatcgcca gattttcttc cggcgtcgtc
     6961 tgaagcactt cggtaacgtg caggagccag ctcttgccga agattgtgca aacgggcgta
     7021 tcggcctcca gcagcgcggc aagcccttta tcctcttccg gcttgatgtt cgcccggcgg
     7081 gttgagccga atgcggcgac cttggcgttc ttgagggatt cctttttaat caggcggaaa
     7141 aactccatgt ctttcgggtt ggatgcagcg aacccgcctt cgatgtaagc gacaccgaat
     7201 tcatcaaggc gcctggcaac ctgcaccttg gcgaccgcgg aaaaggagac cccttccgcc
     7261 tgcgcgccgt cgcgcagcgt cgtgtcatag atggctatgt ttttcttgct catggttttc
     7321 ccctgggaag ccaagaaaga tatacgagcc gaccgcagat ttaaagcctt caggggcggt
     7381 tttttc
//
LOCUS       DAOPZO010000092         7331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_12308_length_7331_cov_5.120533, whole genome shotgun sequence.
ACCESSION   DAOPZO010000092 DAOPZO010000000
VERSION     DAOPZO010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7331
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12308_length_7331_cov_5.120533"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2327
                     /locus_tag="PKI68_06755"
     CDS             <1..2327
                     /locus_tag="PKI68_06755"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNX53428.1"
                     /translation="ANCGLPYHIGGEIAERERLLVTTPEQLRARFRLDVRTRHEVTAI
                     DPATKEVEIKNIETGKNYREPYDALVLSPGAEPVRPPIPGIENKKILTLRNMEDMDRI
                     VKMLDGQKDAAVIGGGYIGLEMAEALRRRKIETTLIELAPQVMGPADPEMVSILHQEL
                     ELHGVDLRLGVSVTAFSDEENGLKLTLSSGATLKTGVAILAIGVKPETALAKAAGLEL
                     GPRGGIKVNDRMQTSDANIYAVGDAVEVTDFITRQPALIPLAGPANRQGRIAADNIFG
                     RDARYRDTQGTAICKVFNLAIGMTGLSEKAAKRAGIAYEKIYVHPASHASYYPGAMPL
                     SMKVLFDPQSGKVLGAQAVGSDGVDKRIDVFAVAIRAGLTVFDLEELELSYAPPYGSA
                     KDPVNFAGFVAANALRGDVKLCHAEDAISPAPHQKLLDVRTSGEFAAGTIPGAKNIPV
                     DELRDRLGELSKDKEYLVFCRVGLRGYLACRILTQNGFSCRNLTGGYITYQRTVEMPA
                     AEMPEDRLPGSDSDEDEDKRETTVKPVKIINACGMQCPGPILQLKHAMDEIKEGEAVS
                     ITATDPGFVSDAPAWCNTTGHELVSIEPAGKGAYCATVVRRVSAPAGVQASGKRAMTN
                     VVFSNDFDKAMAAFIIANGAAAAGYEVTLFFTFWGINILRKAGPVTAKKNLIEKMFGL
                     MMPKGPDRLSLSKMNMAGMGLAMIKGIMKHKSVMSLPELIAQAKLSGVKLVVCTMSMD
                     LMGIKKEELIDGVEEGGVAMYIDRVSRSSANLFI"
     gene            2362..3744
                     /locus_tag="PKI68_06760"
     CDS             2362..3744
                     /locus_tag="PKI68_06760"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX53429.1"
                     /translation="MSSRLFLPVIAVLIVSNVSFAGEEVSHANALSFETFSRKVLEYY
                     PKLKAAHSGVDIALAKQMQAQAGFWPSLNLSAGYTISDDPVNVFGMLLRQERFASSDF
                     ALNRLNAPEAHQDLSAGVHVELPLFDAMQTIGRTRSAREMVKASQADAAFTKMEALLM
                     AQDAYLNAMTFEKLSAVINEVYRSSEEDLQKAKDLKDRGVIFGADYYSAKVMFGDFTR
                     MKNELARQRKAMNILLNILMGEPLNKVWLLTSSVREADTPSQDQEKLIDAAFISRPDV
                     FSLNARLLALESESSRARASALPRVSAFGDVTNDRNGIGDAGGNNYTVGIKAEMPLFD
                     PSRKGRILEAESKKVQLEYDVQLLKDGIRRDITEEFARNSTLRDNVTVLKDMTDDATA
                     AVGLLVPLYSEGRKSIVDLFEVRRAYLQSSQAYDKALIGIRLSEAKLLFLSGRLNENE
                     ISKLEEGVGQ"
     gene            3741..6965
                     /locus_tag="PKI68_06765"
     CDS             3741..6965
                     /locus_tag="PKI68_06765"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX53430.1"
                     /translation="MSSLQEGLIARIVRIFVKSKVTPLIIGFSVFLGVLAVLNLPREE
                     EPQISVPMFDIFVSYPGATAEEVEDRIVNVGERKLWEISGVEYVYSAIQAEGAVITVR
                     FRVGENAEKSLIKLYTKVYSNLDLLAKDGSMPLIKIRSIDDVPVMTVTFHGATNDAVR
                     LRKVVSDVQRSISALPDVSETAITGGRKRQFHVFFDETKLNERFLNPLELTRLVQQAN
                     VKTAAGHLQSRPEIINVEADGFFRSAEDLGNLVVSVSQGKEVYLKDVAEIVDGPDEEE
                     TAVTIRFGCADRSGAEEPFDAVTLSVSKRKGANASRLCEKILTRVDELKGGLIPADVT
                     YTITRNYGETADEKSNELLFHMSIAVVAVTILMALALGWRESGVVAVAIPMTLSLTLA
                     GFYFLGYTLNRITLFALIFSIGILVDDPIVGVENIVRHLRMAKNKGRDFLEVTIEAFK
                     EVQSPLILATLTVIAAILPMAFVRGLMGPYMRPIPIGASMAMVFSMIVAFVATPWAAH
                     KILGGAKRRGLKGHGEQDTLLTRIYRAYMKPLIYSARVRWGFIAVLLVLLSGSILLIP
                     LKLVKVKMLPFDNKNEFQVVLDMPEGASFDQTKIVLNELATYISTVPEVKNITAYAGT
                     SAPYNFNGLVRHYFLRSKPWQADLQVNLTGKSERKRQSHEIASSVRPKVHEIVSKYGG
                     HVQVAEVPPGPPVLSTLVLEVYGPTLEGQRKLAGDIETLLSSNAGVTDVDSYTGRQEK
                     LNRLKIDKQKCSLNGIPVDSVAAIILGSIKGSRADLAHLSGENEPLEIVIRLPKEKRE
                     TLNSVLNVTVLSRYGRKIPVSDLVTVENSLKDLTIYHKNLERVSYVIGDVQGAFDSPI
                     YAMMDLKEGIDKLALPEPKPESQKKPDVLFTGQPQTGDRWSVKWDGEWQITYEVFRDL
                     GIAFMIVLILIFTLVVGWFQSFKTPFIIMLPIPLTLVGILPAHWMTGTFFTATSMIGM
                     IAGAGVVVRNSIILVDFIELRVREGMALEEAVIDAGAKRFRPMLLTATAVVVGASVIL
                     FDPIFQGLALSLIAGEIASTLLSRTAIPMLYYMAVRPKHGVLRR"
     gene            6970..7149
                     /locus_tag="PKI68_06770"
     CDS             6970..7149
                     /locus_tag="PKI68_06770"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNX53431.1"
                     /translation="MERTLRGIAGALLLVSLMLAHYISPLWLWFSAFIGLNLLQSAFT
                     NWCPMMWILKKAGIK"
BASE COUNT         1677 a         1874 c         2103 g         1677 t
ORIGIN      
        1 tcgccaactg cggcctgccg taccacatcg gcggcgaaat cgccgagcgc gagcgtttgc
       61 tggtgaccac gcccgagcag ctgcgcgccc ggttcaggct ggatgtgcgc acccggcacg
      121 aagtcacggc gattgatccg gcgacgaaag aagttgaaat taaaaacatc gagacaggga
      181 aaaattatcg cgaaccgtac gacgcgctgg ttctcagtcc cggtgccgag ccggttcggc
      241 ctccgattcc cggaattgaa aataaaaaga ttctgactct ccgcaacatg gaggatatgg
      301 atcgtatcgt caaaatgctc gacgggcaga aggacgcagc ggttattggc ggcggctaca
      361 tcggtctcga aatggccgaa gcgctgcgcc gccgcaaaat cgagaccacg ctgattgagc
      421 tggcgccgca ggtgatgggc ccggccgatc cggaaatggt ttcaattctt catcaggagc
      481 tggagctgca tggcgtagat ttgcgtttag gcgtttcagt aacggctttt tctgatgaag
      541 aaaacggcct gaagctcaca ctcagttccg gcgcaacgct gaaaaccggc gtggcgattc
      601 tggcgatcgg cgtgaagccg gaaacggcgc tggcgaaagc ggccggtctg gagctcggtc
      661 cgcgcggggg aattaaagtc aacgaccgga tgcagacctc ggacgcgaat atttatgcgg
      721 ttggcgacgc ggtggaagtg accgatttca tcacccgcca gcctgcgctg attccgctgg
      781 ccggtccggc gaaccggcag ggacgcatcg ccgccgacaa tatcttcggc cgcgacgcgc
      841 gctataggga cacgcagggc accgccattt gcaaggtgtt caacctcgcc atcggcatga
      901 ccggcctgag cgaaaaagcg gcgaagcgcg cgggcattgc gtatgaaaaa atctatgtcc
      961 atcccgccag ccacgcctcg tattatccgg gcgctatgcc gctttcaatg aaagtgctct
     1021 tcgacccgca gagcggcaag gtgctcggcg cgcaggccgt cggctcggac ggcgtcgata
     1081 aacgaattga tgttttcgcc gtcgccatcc gcgccggact gacggtgttc gatctcgaag
     1141 aactcgaatt gtcgtatgct ccgccgtacg gctcagccaa agaccctgtc aacttcgccg
     1201 gatttgtcgc ggccaacgcg ctgcgcggcg atgtgaagct ctgccatgcg gaagacgcaa
     1261 tcagtccggc accgcatcag aagctgcttg acgtccgcac atcgggcgag tttgccgccg
     1321 gcactattcc cggcgcgaaa aatattccag tggatgaact gcgcgaccgg ctcggtgagc
     1381 tttcaaaaga taaagaatac cttgtttttt gtcgcgtcgg tctgcgcggc tatctggcct
     1441 gtcgcattct gacgcaaaac ggattcagct gccgcaacct taccggcgga tatattacgt
     1501 atcagcggac tgttgaaatg cctgccgctg aaatgccgga agaccgtctt ccgggaagcg
     1561 attcagatga ggacgaagat aaaagagaaa ccacggtgaa gccggttaaa attatcaacg
     1621 cctgcgggat gcaatgtcca ggcccgattc tgcagctcaa acacgcgatg gatgaaatca
     1681 aagaaggtga agccgtcagt atcacggcca ccgatccggg gtttgtttcc gatgcacccg
     1741 cctggtgcaa caccaccggg cacgagctgg tgagcataga gccggcggga aagggcgctt
     1801 actgcgccac cgtcgttagg cgtgtgtctg cgcccgccgg cgtacaggct tccggaaagc
     1861 gcgccatgac caacgtcgtt ttctcgaatg atttcgataa ggcgatggcc gctttcatta
     1921 tcgccaacgg cgcggcggct gcgggctatg aggtgacgct gttcttcacc ttctggggca
     1981 tcaacattct ccgcaaagcc gggccggtga cggcgaagaa aaacctgatt gaaaaaatgt
     2041 tcgggctgat gatgccgaaa gggccggatc gtctctcgct gtcgaaaatg aacatggcgg
     2101 gtatggggct ggcgatgatc aagggtatca tgaagcataa aagcgtcatg tcgctgccgg
     2161 agctcattgc gcaggcaaaa ctcagcggcg tcaaactggt agtctgcacg atgagcatgg
     2221 atctgatggg aatcaaaaaa gaagaattga ttgacggagt tgaagaaggc ggcgtggcaa
     2281 tgtacattga ccgcgtaagc cggtcatccg ctaatttgtt tatctagatt ttccgggggc
     2341 ggcatacgaa ggagcgtgaa tatgtcatcc agattgtttc tgccggtaat cgccgtttta
     2401 atagtttcca atgtttcatt tgccggagaa gaagtgtcgc atgcaaatgc gttgtcgttc
     2461 gaaacctttt cccggaaggt gcttgagtac tatcccaaac tgaaggctgc tcatagcggg
     2521 gtggatattg ctctggcaaa acaaatgcaa gcccaggcgg ggttttggcc atccctgaat
     2581 ctgtccgccg ggtacaccat cagtgacgat ccggttaatg tcttcggtat gctcctccgg
     2641 caggagcggt ttgcctcttc cgattttgcc ctcaaccggc tgaatgcccc ggaggctcat
     2701 caggatcttt ctgcgggagt gcatgttgaa ctgccgctct ttgacgctat gcagactata
     2761 ggccgaacac gttctgcccg agagatggtg aaggcgtcac aggcggatgc ggcttttaca
     2821 aaaatggaag cgctgcttat ggcgcaggat gcgtatttaa acgctatgac gtttgaaaag
     2881 ctttctgcgg tcattaatga ggtgtacagg agctccgaag aggatttaca gaaggcgaag
     2941 gatctgaaag accggggcgt gatttttggt gccgactatt attcggcaaa ggttatgttc
     3001 ggggatttta cccgtatgaa aaacgagctt gcccgacaga gaaaggctat gaatattctt
     3061 cttaatattc tgatgggaga gccgttgaat aaagtctggc tgcttaccag ctccgtcaga
     3121 gaagccgaca cgccttcgca ggaccaggaa aagctgatag acgccgcgtt catcagccgt
     3181 ccggatgttt tttcgctcaa cgcccgtctt ctggccctgg aatccgaatc gtcccgtgcg
     3241 agagcgtctg cgctgcctcg tgtaagcgca ttcggcgacg tgaccaatga ccgtaatgga
     3301 atcggcgatg ccggcggcaa taactatacc gtcggcatta aagccgaaat gccgcttttt
     3361 gatccttcgc gcaaaggccg cattctggag gccgaatcca aaaaggttca gctggaatac
     3421 gatgtccagc ttcttaagga cgggatccgg cgcgatatta ccgaggagtt tgcccgcaac
     3481 agtacgcttc gagataacgt gaccgtttta aaagacatga ccgacgatgc aacagcagcg
     3541 gtcgggcttt tagtgccgct ttacagcgaa gggcgcaagt ccatcgtcga tctgttcgaa
     3601 gtccggcgtg cctatctgca atcatcacag gcgtatgaca aggcgctgat cggaatacgg
     3661 ctttcggagg caaagctgct gttcctgtcc ggacggttga atgaaaatga aatcagcaag
     3721 ctggaggaag gagtcggcca atgagctcac tgcaggaagg gcttattgct cgcattgtca
     3781 ggatttttgt taaatcgaag gtcaccccgc ttatcatcgg tttttcagtt ttcctgggcg
     3841 ttcttgctgt tcttaatctg ccgagggaag aagaacctca gatttccgtc ccgatgtttg
     3901 acatctttgt ttcctatccg ggagccaccg ccgaagaggt tgaagatcgc attgtaaatg
     3961 tcggcgaacg caaactgtgg gaaatttccg gggtggaata tgtttattcc gcgattcagg
     4021 cggaaggggc tgtgattacc gtgcggttcc gtgtcggcga gaacgcggag aaaagtctga
     4081 tcaaacttta taccaaagtt tactcgaacc tcgatctgct cgccaaagac ggctccatgc
     4141 ctctgataaa aatccgctcg attgatgatg ttccggttat gacggtcacg tttcatggcg
     4201 cgacaaacga tgcggttcgc ctgcgcaagg ttgtgtcaga tgtacagcgc agcatcagtg
     4261 cgcttcctga tgtgtctgaa accgctatta ccggcggacg taaacgtcag tttcatgtgt
     4321 tttttgatga aacgaaactg aatgaacggt ttttaaatcc gcttgagctg acccgtctgg
     4381 ttcagcaggc taacgtcaaa acagcagcag ggcacctgca gagccgaccc gagatcatca
     4441 atgttgaggc ggacggcttt ttccgttccg cggaagatct ggggaatctg gtagtcagcg
     4501 taagtcaggg caaagaagtt tacctgaaag acgtggctga aattgttgac ggcccggatg
     4561 aagaagaaac cgcggttact atccgcttcg gctgcgcaga ccggtccggg gctgaagaac
     4621 cgttcgatgc agtgacgctt tccgtctcaa aacggaaagg agccaatgcc tcgaggctct
     4681 gcgagaagat tctaacaaga gttgatgagt tgaaaggcgg gctcattcct gccgacgtca
     4741 cgtatacaat cacccgcaat tacggcgaaa cggcagacga aaaatccaat gagcttctct
     4801 ttcatatgtc gattgctgtt gtggccgtaa cgatcctgat ggcgctggcc ttgggttggc
     4861 gagagtcggg tgtcgtggcc gtcgccattc cgatgaccct gtcactgact ctggccggct
     4921 tttatttcct cggctatacg ctgaaccgga tcacgctttt cgcgctgatt ttttccatcg
     4981 gtatcctggt cgatgacccg attgtgggcg tcgaaaatat tgtccggcat ctccgcatgg
     5041 cgaagaataa aggacgcgat tttctggagg tgacaattga agccttcaaa gaggtgcaga
     5101 gcccgctgat tcttgccacg cttaccgtga ttgcggccat tctgcccatg gcatttgtgc
     5161 gggggttgat ggggccgtat atgcgcccga tcccgatcgg tgccagcatg gccatggtgt
     5221 tctccatgat tgtggcattt gtcgccactc cctgggcggc acataaaata ctcggcggcg
     5281 caaaacggcg ggggctcaaa ggacacggcg aacaggatac gcttctgaca agaatatacc
     5341 gcgcctacat gaagccgctg atctacagtg cccgggttcg ctgggggttc atcgccgtgc
     5401 ttctggtcct gctgtccggc tcgatactgc ttatccctct gaagctggta aaagtcaaaa
     5461 tgctgccgtt cgacaacaaa aacgaattcc aggtcgtcct ggacatgccg gagggggcat
     5521 cttttgatca gaccaaaatt gtcctcaacg aactggcgac ttatatcagc acggttccgg
     5581 aagtgaaaaa cattacggca tatgccggga caagcgctcc gtataatttc aacggactgg
     5641 tccggcatta tttcctgcgt tctaaaccct ggcaggcaga tcttcaagtc aacctgaccg
     5701 gtaagagcga acgtaaacgc cagagccatg agatcgcgag ctccgttcgc ccgaaggttc
     5761 atgagatcgt ctcgaaatac ggcgggcatg ttcaggtcgc ggaggtgccg ccggggccgc
     5821 cggtgctttc aacgctggtt ctggaggtgt acggcccgac gctggaaggc cagcgcaagc
     5881 ttgccggcga catcgaaacc ctcctcagca gcaatgccgg tgttactgat gttgacagct
     5941 atacagggcg gcaggaaaaa ctgaaccgtt taaaaattga taagcagaaa tgctccttga
     6001 acggtatccc ggtggattcc gtggctgcga tcattctggg ctcaattaaa ggcagccggg
     6061 cggatctcgc gcacctctcc ggcgagaatg aaccgctgga gatcgtcatc agactgccca
     6121 aagagaaacg agaaaccctg aacagtgttt tgaatgtaac cgttttatcc agatatggga
     6181 gaaagattcc ggtcagcgat ctcgtcacgg ttgaaaactc gcttaaagac ctcacgatct
     6241 atcacaaaaa tcttgagcgg gtgtcctatg taatcggcga cgtgcagggg gctttcgaca
     6301 gtccgatcta tgcgatgatg gatttgaagg aaggcatcga caagctggct ctgccggaac
     6361 cgaagcccga atctcagaag aaaccggatg tattgtttac aggtcagccg cagaccggcg
     6421 accgctggtc cgtcaaatgg gatggagaat ggcagatcac ctacgaagtt ttccgcgatc
     6481 tgggaatcgc cttcatgatc gttctcatcc tcatttttac gctggtggtc ggctggtttc
     6541 agtcgtttaa aacacccttc atcatcatgc ttcccatacc cttaacgctg gtgggcatcc
     6601 tgccggcgca ttggatgacc ggtacatttt tcactgcgac ttcgatgatc ggcatgattg
     6661 ccggcgcagg cgtcgtggtc cgcaactcga tcatcctcgt ggatttcatt gaattgcgcg
     6721 tccgggaagg catggcgctt gaagaggccg ttattgacgc cggagccaag agattccgcc
     6781 cgatgctctt gacggcaacg gctgttgttg tcggtgcctc ggttatcctg ttcgatccga
     6841 tctttcaagg gctggcgctg tcgctgatag ccggggagat tgcttcaacg cttttgtcga
     6901 ggacggccat tcctatgctt tactatatgg cagtgagacc aaaacacggt gttttgcgga
     6961 ggtaaaacca tggaaagaac gttgagaggt attgccggag cgcttctgct ggtcagcctg
     7021 atgctggcgc actatatcag cccgctgtgg ctctggttca gcgcttttat cggactgaat
     7081 cttttacagt cggcattcac caattggtgt cccatgatgt ggattttgaa aaaagccgga
     7141 atcaagtgac ctgcataaaa tcaggcgata ttccacggat tatcgccaga gatattgcgt
     7201 tcggaccgta acgcttacag gtggtttttt tcattcagcc aggcctgggt tttccggagc
     7261 atctcctgaa gctttaccag ctggatgggt ttgacgagat aatcatccat tccggcgtca
     7321 agtccgcggg c
//
LOCUS       DAOPZO010000093         7230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_12543_length_7230_cov_8.758606, whole genome shotgun sequence.
ACCESSION   DAOPZO010000093 DAOPZO010000000
VERSION     DAOPZO010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12543_length_7230_cov_8.758606"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PKI68_06775"
     CDS             <1..326
                     /locus_tag="PKI68_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNX53432.1"
                     /translation="RTLSNGYGIKASLANIHTVKPFDAEYLYHRIKTGRPVVTIEEHN
                     IIGGLGSAAAEVIAESGVRVTFKRFALPDEYSHYVGSQEYIRSRWRLDCASLAEEIAS
                     LVQRK"
     gene            340..1329
                     /locus_tag="PKI68_06780"
     CDS             340..1329
                     /locus_tag="PKI68_06780"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX53433.1"
                     /translation="MDTLFSKETSEGVTSFSGGRVLLTGGTGFFGKWLLSLFLFAGKN
                     PDTEIVVLSRNPAQFAAQYPMFGKSSNITFIQGDVLDFSFPAGPFDYVIHAATETNAK
                     LERENPDGLYSVIVDGTRRVLEFSKQAEVKRVLYISSGAVYGVQPPQLLRIPEIFPCN
                     PVTVYGKGKLAAEKMCLESGIETVIARPFAFVGPWLPLDAHFAIGNFIGSCLRNEPIE
                     IKGDGTPLRSYMYGADLSEWLLAILLRGEPSRAYNVGSDEAISIADLARLVRECAGTQ
                     NEIIVHGKKTAGALPARYVPSVDRAKNELSLRLHCSLPDAIRRTIDWHRNLRK"
     gene            complement(1342..2082)
                     /gene="recO"
                     /locus_tag="PKI68_06785"
     CDS             complement(1342..2082)
                     /gene="recO"
                     /locus_tag="PKI68_06785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNX53434.1"
                     /translation="MIVKTPAIPLYWTDVSNTSRIVTWLTAHYGKLSTTIKGDQRKNS
                     LFRGQYDLFSTSELLFYDRTERGAHIAKECSMLHSRSVFRTDWRACAAAGYISALFAK
                     TTPRHGHEPGRFEFFEEMLGLAEEYGRHPAFLVWFDLKFAEFQGQLLQLHDDKRPVYR
                     FAAEYGGIIPPDYARSQRIAAAPLAPESVALLKAWQQTETPGEVLAAVYSPAHFAQIE
                     RVLEKFVTYQFDLPPHVRRTATEILRAA"
     gene            2196..3227
                     /locus_tag="PKI68_06790"
     CDS             2196..3227
                     /locus_tag="PKI68_06790"
                     /inference="COORDINATES: protein motif:HMM:NF016643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53435.1"
                     /translation="MLLNKTIRLFLDSIGRREEYEFYLNKFQSAGTDCFALLVPDLES
                     LEQGAEAIAFDLQFLLKLGLKPAVVLCGADAGAKWAILSNHPLFERTVFPKLGRRKAS
                     VPVFVEENVQTLESLLQKVPVIGKRIHFIRAAGGLSVEYYYTQKPLPALSSDDRLLAD
                     TAAALLEARPGTHISVTAPMNLLEEIFTVKGAGTVFRRGSTILSGRVPDSNRLLALLE
                     ESFGKKPKAGVFSASTHIYCEENYRGAALLEDHPEGLYLSKFAVGPEARGEGLAQELW
                     DEVCKNHPAFFWRSRKGNPINQWYDRQADGRHTAGDWIIFWRGMDAASIPGFIRFCSA
                     RPADFIAAP"
     gene            3414..>3808
                     /locus_tag="PKI68_06795"
     CDS             3414..>3808
                     /locus_tag="PKI68_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53436.1"
                     /translation="MNKKYFIFDIGNVLVNFDFQILLQQIAADSGAPVKQPTERDLEM
                     HHAVEEGVISDQDFVDYLNAAEGLSWTVETLIGVWQKMFTVNQTGYGLFKEAIARGLP
                     VYTLSNIAEHHIEAIERNWPGFLDGATGL"
     assembly_gap    3809..3908
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3909..4174
                     /locus_tag="PKI68_06800"
     CDS             <3909..4174
                     /locus_tag="PKI68_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNX53437.1"
                     /translation="EAIERNWPGFLDGATGLFLSYQVGARKPNAEIYRHMLENLGVKG
                     EQCLFLDDLERNVDAARAAGIHALQFIPENHAAIHKAVGEFFG"
     gene            4464..6383
                     /locus_tag="PKI68_06805"
     CDS             4464..6383
                     /locus_tag="PKI68_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNX53438.1"
                     /translation="MIDFIQVSKRFGTQEVLDKVSFRINSGEHVGVVGPNGAGKSTVF
                     GLICGEQSADSGDVSLPKNVRLGHLHQQLHAHAQDGSLLNYACNAIAELKPMEAEIHR
                     IEHDLQTVSGAEQERLLARLGDLQHEFEHLGGYDLKSRAEAALSGLGFKESEFDRPFR
                     SFSGGWQMRAELVRTLIARPDILLLDEPSNYLDLPAVEWLQRFLRGFEGTMLLISHDR
                     YLLESLTDRTLEVSGGTVTKYSGGYTYYIREREQRHMQQEAAYRNYVEQKEHLESFIS
                     RFRAQATKAAQVQSRIKMLEKIEVVKAPVAPPNLSKLRIPPPPHCGAHIMSVQNLNFS
                     YDGVRWILRDVNLEIGHGQKIGLVGFNGMGKTTLLRLLAGVLAPQSGVRREGHKVVLG
                     YQSQDFAETMPPEQSLLTIVRNANPAVPERDVRGLLGSFGFGGDAVNKPSGVLSGGEK
                     IRLAFARIFINPPNFLLLDEPTTNLDIHGREALEKAIRDYQGTVCFVSHDVAFVRGAA
                     DHIISVADGSVTGFPGGYDYYLEKSAKLDAGNLKPETGKPEAPKSEPKAQSLTPKDSR
                     IARAQEREAQKELRKMEAAMEKLHGEQRALCDQLSSGQADLDYSALNIQLAEITKKLN
                     TLEAKWLEEADRLGV"
     gene            complement(6449..>7230)
                     /locus_tag="PKI68_06810"
     CDS             complement(6449..>7230)
                     /locus_tag="PKI68_06810"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNX53439.1"
                     /translation="GDFVTEADDTIGQLFAALARTGLDSNTLVIVTSDNGPEGSAIAR
                     RTQYNHFSAEKLLGIKRDNWDGGHRVPFIARWPGQVPAGSVSGETICLTDLMATAAAL
                     AGCELPPNAGEDSYNIWPVLAGEKPAAPIREATVHHSSKGLFAIRQGQWKLLTHAGSG
                     GNNYDKDPAYAAYYSKPVQLYDLAADPYEKVNLADSHPEIVAKLTALLRQYVINGRST
                     PGPAMQNDTPNNWKQLEGLNFPVEKASRTKGKKAVNAVSEE"
BASE COUNT         1404 a         1993 c         2219 g         1514 t
ORIGIN      
        1 tgcggacatt gtcgaatgga tatggaataa aggcgtcgct agccaatata catactgtta
       61 aaccttttga tgcggaatat ctgtatcatc ggatcaagac tggccgtcca gttgtcacga
      121 tcgaagagca caatataatc ggagggttag gcagtgcggc tgccgaggtg attgcggaat
      181 caggggtcag agtgacgttt aagcggtttg cactccctga tgaatacagt cattatgtag
      241 gcagtcagga atatatccgt tcccgctgga ggcttgattg tgcgtcgctg gcggaagaaa
      301 tcgcctcgct ggttcaacga aaataattac gagaaccgca tggatacgct tttttctaaa
      361 gaaacttcgg agggtgttac atcattttcc ggtggccggg tcctcctcac cggaggaacg
      421 ggttttttcg gtaaatggct gctttccctt tttttattcg caggcaaaaa tccggatacc
      481 gaaatcgttg tgcttagccg taatccggcg cagtttgctg cgcagtatcc aatgtttgga
      541 aaaagctcga atatcacgtt tattcagggc gatgtgctcg atttcagttt tccggcaggg
      601 ccgttcgact atgtaattca tgcggcgacc gagaccaatg caaagctgga aagagaaaat
      661 ccggacgggc tgtattctgt aatcgtcgat ggaacccggc gggtgcttga attttcaaaa
      721 caggcggaag tcaagcgggt gctttatatc agctccggtg ccgtttacgg tgttcagcct
      781 ccgcagttgt tacgcattcc ggagatcttt ccctgcaatc cggttacagt ttacggaaaa
      841 ggcaagctgg cggcggagaa gatgtgtctt gagagcggca ttgaaacggt tattgcccgg
      901 ccgtttgcct ttgtcggccc gtggcttccg ctggatgcgc acttcgcgat cggcaatttt
      961 atcggtagct gtctgcggaa cgaaccgatt gaaatcaagg gtgacggtac accgctgcgc
     1021 tcctacatgt acggcgctga tttgtcggaa tggctgctgg cgattttact gcgcggtgaa
     1081 ccgtcacggg cctataacgt aggttctgac gaagcgattt ccattgccga tctggcccgt
     1141 ctcgtccgtg agtgcgccgg aacgcaaaac gaaattatcg ttcacggaaa gaaaactgcg
     1201 ggagccttgc cggcgcgcta tgtgccgtcg gttgatcgcg caaaaaatga actgtcttta
     1261 cgtttgcact gctctttgcc ggacgcgatc cgccggacga tcgactggca ccgcaactta
     1321 cggaaataga cggttctttt tttatgcggc gcgaaggatt tcagttgccg tgcggcggac
     1381 gtgcggcggc aggtcgaact ggtaggtgac gaatttttcc agtacgcgtt cgatctgggc
     1441 aaagtgcgcc ggactgtaaa ctgccgccaa aacctcgccg ggagtttccg tctgctgcca
     1501 cgctttgagc agggcgacgg attccggcgc gagcggcgcg gcggcgatgc gctgcgagcg
     1561 ggcgtagtcg ggcgggatga tgccgccgta ttcggcggcg aagcggtaga ccgggcgttt
     1621 atcgtcgtgc agttgcagca gctggccctg gaattccgcg aacttcaggt cgaaccagac
     1681 gaggaaggcg ggatggcggc cgtactcttc ggccagaccg agcatttcct caaaaaactc
     1741 aaaccggccc ggttcgtggc cgtggcgcgg ggtggttttt gcaaacagcg cggaaatata
     1801 accggcggcg gcgcaggcgc gccagtccgt gcggaaaacg cttcgcgagt gaagcatgga
     1861 gcactctttg gcgatgtgtg cgccgcgttc ggtgcggtca tagaagagca gttcgctggt
     1921 cgagaacaga tcgtactgtc cgcggaagag gctgtttttg cgctggtcgc ctttgatggt
     1981 tgtggagagc tttccgtaat gcgcggtcag ccaggtgacg atgcgcgaag tgttcgagac
     2041 atcggtccag tagagcggaa tagctggcgt tttaacgatc atggcgggcg ggcgacttac
     2101 taaccggacg catcctaact gcaacccgtc cggccgccaa gcttcggctg gcatttttga
     2161 tgtacttttc cggcgaatga tgtttccttc tgcgcatgtt actgaacaaa accatacggc
     2221 tgtttctcga cagcatcggc cggcgcgagg aatacgaatt ttacctcaac aaatttcagt
     2281 ctgcgggcac cgactgcttc gcgctgctgg tgccggatct cgaaagcctt gaacagggcg
     2341 cggaggccat tgccttcgac cttcagttcc tcctgaagct cggcctgaag ccggcggttg
     2401 tgctttgcgg tgcggatgcc ggcgccaagt gggccatcct cagcaaccat ccgctgtttg
     2461 agcggacggt ttttccgaag ctcggccggc gcaaagccag cgtacccgtt tttgtcgaag
     2521 aaaatgttca gacgctggaa agcctgctgc agaaagttcc ggtcatcggc aaacgcatcc
     2581 acttcatccg cgccgccggc ggactctccg tggaatacta ttacacccag aaaccgctgc
     2641 ccgcgctttc gtccgacgac cggctgctgg ccgataccgc cgccgcactg ctcgaagcgc
     2701 ggcccggcac ccacatatcc gtcacggcgc cgatgaacct gcttgaggaa attttcaccg
     2761 tcaaaggcgc ggggacggtc ttccggcgcg gttcaaccat tctttccggc cgggtgccgg
     2821 attcaaaccg gctgctggcg ctgctggaag aaagcttcgg caaaaagccg aaagccggtg
     2881 ttttctcggc ttccacgcac atctattgcg aagaaaatta ccgcggtgcg gcattgctgg
     2941 aagatcatcc ggaaggactc tacctctcca aattcgctgt cggcccggaa gcgcgcggcg
     3001 aaggtctcgc tcaggagctc tgggacgaag tctgcaagaa ccatcccgcc ttcttctggc
     3061 ggtcgcgcaa aggcaacccg atcaaccagt ggtacgaccg gcaggccgac ggacggcaca
     3121 ccgcgggcga ctggattatc ttctggcgcg gtatggacgc cgcttcaatt ccgggcttta
     3181 tccgcttctg ttccgcccgt ccggcggatt ttattgcggc cccgtagtca ggcgtggagc
     3241 gggcgctccg cgcatgcttg ctttaacgtg ccgggacggc acgttccacc ttctgcactt
     3301 ggcaggcagg atgcctgcgg tacgtatcgc cgccgtcccg gcggccttgc tgcataatga
     3361 gtgatgttgc ggattgccgc agccgccgga tgccgctaca ttttccggct tctatgaaca
     3421 aaaaatactt catcttcgac atcggcaacg tgctggtgaa ttttgacttc cagattctgc
     3481 tccagcagat cgccgccgac tccggcgcgc cggtcaagca gccgacggaa cgcgacctcg
     3541 aaatgcacca cgcggtcgaa gagggcgtca tcagcgatca ggactttgtc gactacctga
     3601 atgccgccga aggactttcc tggacggtgg agaccctgat cggtgtctgg cagaaaatgt
     3661 tcaccgtcaa ccagaccggc tacggactgt tcaaagaagc catcgcccgc ggcctgccgg
     3721 tttacacgct cagcaacatc gccgaacacc acatcgaagc gattgaacgc aactggcccg
     3781 gctttttgga cggcgcgacc ggactgttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnntc gaagcgattg aacgcaactg gcccggcttt ttggacggcg cgaccggact
     3961 gttcctttcc tatcaggttg gcgcccgcaa acccaatgcg gaaatttacc ggcacatgct
     4021 ggaaaacctc ggcgtcaaag gtgaacaatg cctgtttctc gatgacttag agcgcaacgt
     4081 cgacgccgcc cgcgccgccg gaatccacgc cttgcagttc atccccgaaa accacgccgc
     4141 catccataaa gcggtcggtg aattttttgg gtaacgttgc gtgtgacctg cgcgggacac
     4201 aaactgcagg tatgaaattg tgcgtttgtc aaccggccgg gctggtctca aataaaggtg
     4261 gaacgcgatc tccgagcgcg ttctgaacct gcccggaggt caggttccac ctcaatgtgg
     4321 agccaacgtt acaatcaagg ccgccaggat ggcggcggta cgtaccgcag gcatccttgc
     4381 ctgccttggt gttcgcgttc gtttgcgcgg attagcggtt taggtttcca aacaggggat
     4441 gaaccgctac attccccggc tgcatgattg attttatcca ggtgagcaaa cggttcggca
     4501 cgcaggaggt gctggacaag gtgtcgttcc gcatcaattc cggcgagcat gtcggcgtgg
     4561 tcgggccgaa cggcgcgggc aagagtacgg tgttcgggct gatctgcggc gagcagtcgg
     4621 ccgacagcgg cgatgtctcg ctgccgaaga atgtgcgcct cggccatttg caccagcagc
     4681 tgcacgccca cgcgcaggac ggctcgctgc tgaattatgc ctgtaatgcg attgcggaac
     4741 tcaagccgat ggaggcggag attcaccgca ttgagcacga tttgcagacg gtttccggcg
     4801 cggaacagga gcggctgctg gcgcggcttg gcgatttgca gcacgagttc gaacatctgg
     4861 gcggctacga tctgaagtcg cgcgccgaag ccgcactgag cggactcggc tttaaggaga
     4921 gtgagttcga ccgcccgttc cgctcgttca gcggcggctg gcagatgcgc gccgagctgg
     4981 tgcgtacgct gattgcgcgg cccgatattc tgctgctcga cgagccgtcc aactatctcg
     5041 acctgccggc cgtcgaatgg ctccagcgct ttctgcgcgg cttcgaaggc acgatgctgc
     5101 tgatttccca cgaccgctat ctgctggagt cgctgacgga ccgcacgctg gaagtcagcg
     5161 gcggcacggt cacgaaatat tccggcggtt acacctatta catccgggag cgcgagcagc
     5221 gccacatgca gcaggaagcg gcttaccgaa actacgtgga gcagaaagag catctcgaaa
     5281 gtttcatcag ccgtttccgc gcgcaggcga ccaaagcggc gcaggtgcag agccgcatca
     5341 aaatgctcga aaagatcgaa gtggtaaaag cgccggttgc gccgccgaat ctgtcgaagc
     5401 tgcggattcc gccgccgccg cactgcggcg cgcatattat gtcggtgcag aatctgaatt
     5461 tttcgtatga cggcgtgcgc tggattcttc gcgatgtgaa tctggagatc ggccacggcc
     5521 agaaaatcgg gctggtcggg tttaacggca tgggcaaaac gacgctgctg cggctgctgg
     5581 cgggtgtgct ggcgccgcag tcgggcgtgc gccgcgaggg gcataaagtg gtgctcggct
     5641 accagtcaca ggactttgcc gaaacgatgc cgccggagca gtcgctgctg acgattgtcc
     5701 gcaatgcgaa tccggcggtg ccggagcgcg atgtgcgcgg actgctcggt tcgttcgggt
     5761 tcggcggcga cgcggtgaat aaaccctccg gcgtgctcag cggcggcgaa aaaatccgcc
     5821 tggcgtttgc gcgcatcttt atcaatccgc cgaattttct gctgctcgac gagccgacga
     5881 ccaacctcga catccacggc cgcgaggcgc tggagaaggc gatccgcgac tatcagggca
     5941 ccgtctgctt cgtcagccac gacgtggcgt ttgtgcgcgg tgcggccgat catattattt
     6001 cggttgccga cggctccgtc accggcttcc ccggcggcta cgattactat ttggagaagt
     6061 ccgcgaaact tgacgcggga aacttgaagc ctgaaacagg gaaaccggaa gctccgaaat
     6121 ccgagccgaa agcccaaagc ctgacgccga aggactcacg aattgcccgc gcgcaggagc
     6181 gcgaggcgca gaaggagttg cgcaagatgg aggcggcgat ggagaagctg cacggcgaac
     6241 agcgcgcgct gtgcgaccag ctttccagcg ggcaggcgga tctcgactac agcgcgctga
     6301 atatccagct cgcggaaatc accaaaaagt tgaatacgct cgaagcgaag tggctggaag
     6361 aggccgaccg cctcggcgtg tgagaaagaa gggttttgcc gggcgatagc aaaagagcgc
     6421 ggtttgcacc gcgctctttg ccgcagagtt actcttcgct gacggcgttg acggcctttt
     6481 tgcctttggt tctcgacgct ttttcgacag ggaagttcaa cccctcgagc tgtttccagt
     6541 tgttgggcgt gtcgttctgc atggccgggc cgggggtgct gcgcccgttg atcacgtact
     6601 gccgcagcag cgcggtcagt ttcgcgacga tctccgggtg gctgtcggcc aggttgacct
     6661 tttcatacgg gtcggcggcc aggtcgtaga gctgaaccgg tttgctgtag taggccgcat
     6721 aggccggatc tttgtcataa ttgttcccgc ccgaaccggc atgcgtcagc agtttccact
     6781 gtccttggcg aatagcgaag agacctttgc tggagtggtg caccgtggct tcgcggatcg
     6841 gcgcggccgg tttttcgccg gccagtacgg gccagatgtt atagctgtct tcaccggcgt
     6901 tcggcggcag ctcacagccg gccagcgcgg cggccgtggc catcaggtcg gtcaggcaga
     6961 tggtttctcc gctgacgctg ccggccggaa cctgtccggg ccagcgggcg atgaacggaa
     7021 cgcgatggcc gccgtcccag ttgtcgcgtt tgatgccgag cagtttttcc gcgctgaagt
     7081 gattgtattg cgtcctgcgg gcgatggcgg agccttcggg gccgttgtcg ctggtgacga
     7141 tgaccagagt attggagtcg aggccggtcc gggcaagcgc cgcgaaaagc tgcccgatcg
     7201 tgtcgtcggc ttcggtcaca aagtcaccgt
//
LOCUS       DAOPZO010000094         7110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_12857_length_7110_cov_9.997165, whole genome shotgun sequence.
ACCESSION   DAOPZO010000094 DAOPZO010000000
VERSION     DAOPZO010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7110
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12857_length_7110_cov_9.997165"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2282
                     /locus_tag="PKI68_06815"
     CDS             <1..2282
                     /locus_tag="PKI68_06815"
                     /inference="COORDINATES: protein motif:HMM:NF020928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNX53440.1"
                     /translation="VPFVSTDLKIGPRQVAAVAKLFAEGATVPFIARYRKEAHGNLDE
                     VQILNIQEKLTYYNTLESRRVTILSSINEQGKLTDELREKIERTVSKTELEDLYLPYK
                     PKRRTRATIAKEKGLEPLADLIREQSTESQPLTAAATFINAEKEVATAEEALAGARDI
                     VAEQIAERADVRALIREAYFKTGIIASEPVKADAKPETSKYKDYFDFSQAISEIPSHR
                     YLAIRRGETEGELRRKLVIDADRQIERMKELCGLNPASPFAGELEKTIQDAYKRMLTL
                     SVEIDVSVEMKMKADRAAVEVFAKNLRSLLLAAPYGEKPVIGIDPGLRTGCKCVAIDA
                     TGKYLDTVTIYPDQGAEKTQQAQVALAKFIEKHQPQAIAVGNGTAGRETEAFVKKLLV
                     LMQKKNISVVSVSEAGASVYSASEIARDEFPDLDLTIRGAISIARRLQDPLAELVKTD
                     PKSIGVGQYQHDVYQELLKEKLDEVVVSCVNRVGVELNTASAPLLSRVAGIGESLAKK
                     IVAYRNEHGAFKSRQELMKVAGLGPRAFEQAAGFLRIHGSENPLDASAVHPERYKLVE
                     QIAEDLGEPLNKLVGNVEIVSKIKIEKYTGGDVGELTLKDIVDELKKPGRDPREQFEE
                     VGFRDDVMTMNDLKEGMELKGVVTNVTAFGAFVDIGVHQDGLVHISQLCDRYISDPSE
                     AVQAGDRITVRVLEVDLERKRISLTARKDGGKTAPAKGAAPQRDSRPQGNGPRPQQPQ
                     NRNQRPAPRGFSNNPFAGL"
     gene            complement(2339..3787)
                     /locus_tag="PKI68_06820"
     CDS             complement(2339..3787)
                     /locus_tag="PKI68_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric [FeFe] hydrogenase"
                     /protein_id="HNX53441.1"
                     /translation="MNRYDNQAKRIRKEVLFRVAKAFMGDHPQEEINRIPAEMRPKDG
                     DSSRCCIYKDREVLKYRIMAALGFGVEDEKDELTPLRDYFDRALHRAAVENTGLTVID
                     IACSACLSSRYLVTNACRGCLGRPCQTNCPKDAVEVVGGRAVIHPDRCVNCGLCLKAC
                     PYNAIVRTPVPCEEVCPVNAISKNAAGREEIDHDKCIDCGQCVRACPFAAIEERSQMI
                     DVLKALQDGRKPVALLAPAVVGQFPATLSQIAAALEQLGFCRTEEVAVGADETVRREA
                     AEWQERIQAGAPFMTTSCCPAYVKAVNKHLPELKEFVSHTPSPMAISARMAKEASPEK
                     LTVFIGPCIAKRAEGLNNPDVDYVLTFEELGAIFDTAGIDLRTYPSKPIEPAAQNGGR
                     GFPVSGGVTAAIREHLAEHAAELKPVCINGLDSKALARLQAAVTRGCDGNFIEVMCCE
                     GGCMNGPGVLSNPVVASRKLAELLAEKPKTSA"
     gene            complement(3818..>4429)
                     /locus_tag="PKI68_06825"
     CDS             complement(3818..>4429)
                     /locus_tag="PKI68_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNX53442.1"
                     /translation="KEMVRRISAVSALPDLPYEVVLIQTNVVNAMAAPGGKIMVYEGL
                     WNPKDGLVKDDNELAAVLAHEIAHVNCRHSTEAMTQATWVNGAAAAGLFAAKDTKYQG
                     IAQLAATGGLIAYNGLWVTHYSRKDETEADAVGLMYMAKAGYDPRAALRIWERAAQHH
                     AGGDPADSIFSTHPADSVRLQNLRAQLPAAQAAYAASPYRKAQ"
     assembly_gap    4430..4529
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4707..6218)
                     /locus_tag="PKI68_06830"
     CDS             complement(4707..6218)
                     /locus_tag="PKI68_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53443.1"
                     /translation="MGQTWFKLQNGSDVRGVALEGVPGEPVTLTPEIVRPIGYAFAQW
                     LAEKKGVPAGQLKVAVGHDSRLSAEKIKTAVFQGLRKAECAVADSGLSSTPAMFMATV
                     FENHAYDGSIMLTASHLPFNRNGLKFFTRDGGLGKDDIKKILTIATETGLFDSKCWKK
                     VSKINLMDDYAASLVTTIRTGAGLGDKPLAGLKIVVDAGNGAGGFFVDKVLKPLGANT
                     KGSQFLDPDGRFPNHVPNPEEKAAMDAIISAVEKNGADLGIIFDTDVDRAGAVDRNGR
                     PINRNRFIALMASIVLEEHPGTVIVTDSVTSTGLKYWIEEKLGGVHLRFKRGYKNVIN
                     ESIRLNNEGKESWLAMETSGHGALKENYFLDDGAYQIAKVLIKVAQLHAAGKGSVDEM
                     LAGLPEPAEAREFRAKIKAEDFGAYGGEVLKKFEQFVADEPGWSITPNSFEGVHVTCD
                     AAHGNGWALLRKSLHDPVLPLNIESEEDGGVAKIAARMKTFLSQFDQLELPKL"
     gene            complement(6236..>7110)
                     /locus_tag="PKI68_06835"
     CDS             complement(6236..>7110)
                     /locus_tag="PKI68_06835"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53444.1"
                     /translation="EMSYNNYVDGEGALEAVKELSGKPGVAVIKHTNPCGYATGATLA
                     EAFEAAWNGDPVSAFGSVIAVTQKVDMATAELLKGRFIEALIAPDFDADALELLKSKS
                     KNIRLLKLRQPMSKAGPRKMIKDINGGILVQDVDTDAIAEWKNVTQTAFDQSKRALAE
                     FGIAAAKHTKSNAIMIVQEYKPGQFRVLGMGAGQPNRVDSLRKLAVPRAVENLKNEKP
                     GITDAEIAKAIGECVFVSEAFFPFADSIDEAHAVGARYIVQPGGSIRDDEVIAACDKY
                     GIAMAFTGMRHFKH"
BASE COUNT         1385 a         2223 c         2046 g         1356 t
ORIGIN      
        1 ccgtcccgtt tgtttccacc gacctgaaga tcggcccgcg ccaggtggcc gccgtcgcca
       61 aacttttcgc cgaaggggcg accgtcccgt tcattgcccg ctaccgcaag gaagcgcatg
      121 gcaatctcga tgaagtgcag attctgaaca ttcaggaaaa gctgacctac tacaacacgc
      181 tggagtcgcg ccgcgtgacg atcctcagct cgatcaacga acagggcaag ctgaccgacg
      241 agctgcgcga gaagatcgag cggacggttt ccaagaccga actcgaagac ctttacctgc
      301 cctataagcc gaaacgccgc acccgcgcga cgatcgccaa ggaaaaaggg ctcgaaccgc
      361 tggccgacct gatccgcgag cagtcgaccg aaagccagcc gctgacggcc gccgctacgt
      421 tcatcaacgc cgaaaaagaa gtcgccaccg ccgaagaggc gctggccggc gcgcgcgaca
      481 tcgtcgccga gcagatcgcc gagcgggccg acgtccgcgc gctgatccgc gaagcctact
      541 tcaaaaccgg catcatcgcc tcggaaccgg tcaaggcgga cgccaaaccg gaaacctcca
      601 aatacaaaga ctatttcgac ttcagccagg ccatttccga aatcccgtcg caccgctatc
      661 tggcgatccg ccgcggcgag accgaaggcg aactgcgccg caaactggtc atcgatgccg
      721 accggcagat cgagcgcatg aaggaactgt gcggcctcaa ccccgcgtcg ccgttcgccg
      781 gcgagctcga aaaaaccatt caggacgctt ataaacggat gctgacgctc agcgttgaaa
      841 tcgacgtttc ggtcgaaatg aagatgaagg ccgaccgcgc cgccgtcgag gtgttcgcca
      901 aaaacctgcg cagcctgctg ctggccgcgc cgtacggcga gaaaccggtg atcggcatcg
      961 accccggcct gcgcaccggc tgcaaatgcg tggcgattga cgccaccggc aaatacctcg
     1021 acaccgttac gatctatccg gaccaaggcg ccgagaaaac ccagcaggcg caggtcgcgc
     1081 tggcgaaatt catcgaaaag caccagccgc aggccatcgc cgtcggcaac ggaacggccg
     1141 gacgcgaaac ggaagcgttt gtgaaaaagc tgctcgttct gatgcagaag aaaaatatca
     1201 gcgtggtttc cgtcagcgaa gccggcgcgt cggtttacag cgcgtcggaa atcgcccgcg
     1261 atgagtttcc ggacctcgac ctgaccatcc gcggcgcgat ctcgatcgcc cgccggttgc
     1321 aggatccgct ggcggaactg gtgaagaccg atcctaagtc gatcggcgtc gggcagtacc
     1381 agcacgatgt gtatcaggag ctgctgaagg aaaagctcga cgaagtggtc gtcagctgcg
     1441 tgaaccgcgt cggcgtggaa ctcaacaccg ccagcgctcc cctgctctcg cgcgtcgccg
     1501 gaatcggcga aagcctcgcc aaaaagattg tggcctaccg caacgagcac ggcgcgttta
     1561 agagccgtca ggagctgatg aaagtcgccg gcctcgggcc gcgcgcgttt gaacaggcgg
     1621 cgggcttcct gcggattcac ggcagcgaaa atccgctgga tgcttcagcg gttcatccgg
     1681 agcgctataa actggtcgag cagattgccg aagacctcgg cgagccgctc aacaagctgg
     1741 tgggcaacgt cgagatcgtc agcaaaatca aaattgaaaa gtataccggc ggcgatgtcg
     1801 gcgagctgac gctgaaagac attgtcgacg aactgaaaaa gccgggccgc gacccgcgcg
     1861 agcagttcga agaagtcggc ttccgcgacg acgtgatgac gatgaacgac ctcaaggaag
     1921 gcatggagct caagggcgtg gtcaccaacg tcaccgcctt cggcgcgttc gtcgatatcg
     1981 gcgtgcatca ggacggcctc gttcacatct cacagctctg cgaccgctac atcagcgacc
     2041 cgtccgaagc cgtacaggcc ggcgatcgca tcacggtgcg cgtgctggaa gtggatctgg
     2101 agcgcaagcg catttcgctg acggcccgca aggacggcgg caaaaccgct ccggccaaag
     2161 gcgccgcgcc gcagcgcgac agccgtccgc aaggcaacgg cccgcgcccg cagcagccgc
     2221 agaaccggaa tcagcgcccg gcgccgcgcg ggttcagcaa caatccgttc gccgggctgt
     2281 aagaagaggc cgggatccgg gcgacaccgg atcccggttg ttttgaaacc ggcaggatct
     2341 aggccgaggt cttcggcttt tccgccagca gttcagccag cttgcggctg gccacgaccg
     2401 gattgctgag aactcccggg ccgttcatgc agccgccttc gcagcacatc acttcgataa
     2461 aattgccgtc gcagccgcgc gtcacagccg cctgaagccg ggccagcgct ttgctgtcca
     2521 gcccgttgat acagacgggc ttgagctccg ccgcatgttc cgccagatgc tcgcggatcg
     2581 cggcggtgac gccgccgctg acgggaaatc cgcgcccgcc gttttgcgcc gccggttcga
     2641 tcggcttcga cggataggtc cgcagatcaa ttccggcggt atcaaaaatc gcgcccagct
     2701 cttcgaaggt cagtacataa tcgacgtccg ggttgttcag cccctccgcc cgtttggcga
     2761 tgcagggccc gatgaataca gtcagcttct ccggcgaggc ctccttggcc atgcgggcgc
     2821 tgatcgccat cggcgacggc gtatgcgaaa caaactcctt cagctccggc agatgcttgt
     2881 tcaccgcttt aacataggcc ggacagcagg aggtggtcat aaacggtgcg ccggcctgaa
     2941 tccgctcctg ccattcagcc gcttcgcgcc ggacggtttc gtcggcgccg acggccacct
     3001 cttcagtccg gcagaagccg agctgttcga gggcggcggc gatctggctc agcgtggcag
     3061 gaaactgtcc aaccacggcc ggcgccagca gcgcaacggg tttccgtccg tcctggagag
     3121 ccttaagaac atcgatcatc tgcgaacgct cttcaatggc cgcaaacgga caggcccgga
     3181 cacactgtcc gcaatcgatg catttgtcgt gatcgatctc ttcgcggccg gcggcgtttt
     3241 tggaaatggc attgaccggg cacacttcct cgcacggaac cggcgtgcgc acaatcgcgt
     3301 tatagggaca cgccttcaga cagagtccgc agttgacgca ccggtccgga tgaatcaccg
     3361 cgcggccgcc gacgacctcc acggcatctt tcgggcagtt ggtctgacag ggccttccca
     3421 gacagccgcg gcaggcgttt gtcaccagat agcggcttga gagacaggcg ctgcaggcga
     3481 tgtcaatcac cgtcagtccg gtgttctcca ccgctgcacg atgcagcgcc cggtcaaaat
     3541 aatcccgcag cggcgtcagc tcatcctttt catcctcaac gccgaacccc agagccgcca
     3601 tgatgcggta tttgagcact tcgcggtctt tataaataca gcagcgcgat gagtcgccgt
     3661 ccttcggccg catctcggca ggaatccggt tgatctcctc ctgcggatga tcgcccataa
     3721 acgccttcgc cacgcggaat aaaacctctt tgcgaatacg ctttgcctga ttgtcatacc
     3781 ggttcatcgc tatcctcttg gtcgctcgag ttcctgctta ctgcgctttc cggtacgggc
     3841 tggccgcata ggccgcctgt gccgcgggca gctgcgcgcg cagattctgc agacggacgc
     3901 tgtcggccgg atgggtcgaa aaaatactgt cggccgggtc gccgccggcg tgatgctgtg
     3961 cggcccgctc ccagatgcgc agagccgcgc gcggatcgta gccggctttc gccatataca
     4021 tcagcccgac ggcgtccgct tcggtttcgt ccttgcggga atagtgcgtc acccagaggc
     4081 cgttataggc gatcagcccg ccggtggccg caagctgggc gataccttgg tacttcgtat
     4141 cttttgcggc gaacagaccg gcggccgcgg ctccgttcac ccaggtcgcc tgcgtcatcg
     4201 cctcggttga atggcggcag ttgacgtgcg cgatctcgtg cgccagcacg gcggccagct
     4261 cattgtcatc cttaaccagc ccgtccttcg ggttccacag tccttcatag accatgatct
     4321 tgccgccggg tgcggccatt gcgttgacaa cattggtttg gatcagcacc acttcatagg
     4381 gcagatccgg cagcgcggag accgcgctga tgcggcggac catctccttn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gatcgcacac cgcaccgaga catccggttt
     4561 cctttttcag ttcgtccagc agctgcggat cgcggccggt ggcaaaaacg gtgaggccct
     4621 gcgcgcagag ctgcttaacg gcctcacgtc cgattccgcg cgttgcacct gtcaccaaaa
     4681 ccgtccggtt cataaaacgc cccttattat agtttaggca gttcaagctg atcaaactgc
     4741 gacaggaagg ttttcatgcg cgccgcgatt ttcgcaacgc cgccgtcctc ttcgctttcg
     4801 atgttcagcg gcagaaccgg atcgtgcagc gatttccgca gcagcgccca gccgtttccg
     4861 tgagccgcgt cacaggtgac gtgaactcct tcgaaactgt tcggcgtaat cgaccatccc
     4921 ggttcgtccg ctacaaactg ctcaaacttt ttgagcactt cgccgccgta tgcgccgaaa
     4981 tcctctgctt tgatcttcgc gcggaattcc cgggcttcgg cgggctcagg cagcccggcc
     5041 agcatttcgt caacggaccc tttgccggca gcatgaagct gcgcaacttt gatcagcacc
     5101 ttggcgatct gataggcccc gtcgtcgaga aaatagtttt ctttcagcgc gccgtgaccg
     5161 gaggtttcca tcgccagcca ggattctttg ccttcgttat tcagccggat cgattcgttg
     5221 atgacgtttt tatagccgcg tttgaagcgc aggtgaacgc cgccgagttt ctcttcgatc
     5281 cagtatttca agccggtcga agtcaccgag tcggtcacga tcaccgtgcc gggatgttct
     5341 tcgagaacga tggaagccat cagcgcgata aagcggttgc ggttgatcgg cctgccgttc
     5401 ctgtccaccg cgccggcgcg gtctacatcc gtgtcgaaaa taatgccgag gtcagcgccg
     5461 tttttttcca cggcagagat aatggcatcc attgccgctt tctcttccgg attcggaacg
     5521 tggttcggaa agcggccgtc cggatcaaga aactggctgc ctttggtatt cgcacccagc
     5581 ggcttcagca ccttgtcgac aaaaaacccg cccgcaccgt tgccggcatc gacaacaatc
     5641 ttcagcccgg ccagcggctt atcgcccagt cccgcgccgg tacggatcgt cgtcaccagc
     5701 gacgcggcgt aatcatccat cagattgatt ttggaaacct ttttccagca cttcgaatcg
     5761 aacagaccgg tttctgtcgc aatggtcaga atttttttga tgtcgtcttt tccgagtccg
     5821 ccgtcgcggg tgaaaaactt cagcccgttg cggttgaacg gcagatggct ggcggtcagc
     5881 ataatcgagc cgtcgtaggc gtggttctca aacaccgtcg ccatgaacat cgccggcgtc
     5941 gaagaaagcc cgctgtcggc aaccgcgcat tccgctttgc gcagaccctg aaaaaccgcc
     6001 gtcttgatct tttccgcgga aaggcgcgaa tcgtggccga ccgcgacttt caactgaccg
     6061 gccggaacgc ctttcttttc cgccagccac tgcgcgaacg catagccgat cgggcggaca
     6121 atttccggtg ttaaagtaac cggctcaccg ggaacacctt cgagcgcaac accgcgaaca
     6181 tcactgccgt tctgaagttt aaaccaggtc tggcccatga ttcgaatccc cgattttagt
     6241 gtttaaaatg ccgcatgccg gtgaaggcca tggcgatgcc gtatttatcg caggccgcga
     6301 tgacttcatc gtcgcggatc gatccgccgg gctgaacgat gtagcgcgcg ccgacggcgt
     6361 gggcttcgtc aatgctgtcg gcgaacggga agaacgcttc ggagacaaag acgcactcgc
     6421 cgatggcctt ggcgatttcc gcatccgtga tgcccggttt ttcattcttc agattttcaa
     6481 cggcgcgcgg aaccgccagc ttgcggagcg aatcgacgcg gttgggctga ccggcgccca
     6541 tgccgagcac gcggaactga cccggcttgt attcctgcac gatcatgatc gcgttggact
     6601 tcgtgtgctt ggcggcggca ataccgaatt ccgccagcgc ccgcttcgac tggtcgaacg
     6661 cggtctgggt gacatttttc cactcggcga tcgcgtcggt atccacatcc tgcacgagaa
     6721 tcccgccgtt gatatccttg atcatcttgc gcgggccggc tttcgacatc ggctggcgca
     6781 gcttgagcag gcggatgttt ttgctcttgg acttcagcag ttcgagcgcg tcggcgtcga
     6841 aatccggcgc aatcagcgct tcaataaagc ggcccttgag cagctcggcg gtcgccatgt
     6901 ccactttctg cgtcacggca atcacggaac cgaaagcgga tacgggatcg ccgttccagg
     6961 cggcttcgaa ggcttccgcc agcgtcgcgc cggtggcgta gccgcacggg ttggtgtgct
     7021 tgatcaccgc cacgccgggc ttgccggaaa gttctttgac ggcttcgagc gcgccttcgc
     7081 cgtcgacata gttgttgtag ctcatctctt
//
LOCUS       DAOPZO010000095         6954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_13254_length_6954_cov_8.245833, whole genome shotgun sequence.
ACCESSION   DAOPZO010000095 DAOPZO010000000
VERSION     DAOPZO010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6954
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13254_length_6954_cov_8.245833"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1614)
                     /locus_tag="PKI68_06840"
     CDS             complement(<1..1614)
                     /locus_tag="PKI68_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNX53445.1"
                     /translation="MKTSVKGLSLSILGLCLGSSEAASTPPTKPNILFIMSDDHTAQA
                     IGAYDGRFAALNPTPALDRLAREGMRLDRVFCNNSICTPSRASILTGQYSQANGVLDL
                     TGSLATDHQYLPQLMHEAGYNTALIGKMHLGHVEPNFDYYMVLPGQGEYFDPKFFKKG
                     VGAFEKQPIQIKGYVTDIITDAALDWLKNRDRSKPFFFCLHNKAPHDDFDYAPRYENY
                     LADVTFPEPDSLTNAGNHGSIATRGDQDELVRYIGASIGRRNVFRNQLPLAGLKNNTD
                     MDDEKILHESYQRYIRKYLRCVKGVDDNVQRVVDYLKQEGILDNTLIIYTSDQGMWLG
                     EHDYIDKRWMYEESMRMPMIVRFPGVVPAGTRSSALINNTDFAPTILDFAGVKPPAYM
                     HGRSIRPILETGKTPDDWRKATYYRYWMHLAHHWNPAHFGIRTDRYKLIFFYGLKNDG
                     TGIQTPPGWEFYDLEKDPTEMNNLYDDPACKPVIADLKQQLLELRNQYDETDRKYPAI
                     QAVIDRHWNTTDESRAEAVQISHQAKAAFE"
     gene            1927..3849
                     /locus_tag="PKI68_06845"
     CDS             1927..3849
                     /locus_tag="PKI68_06845"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNX53446.1"
                     /translation="MANRLKRLIGLSLFLLIATFRTGCAETAGTFQAGESDFLLNGKP
                     FVIRSGELHYSRVPREYWQHRLQMCKAMGFNTVCAYMFWSVHEPEPGQFDFSGPADVA
                     AFCRLAQQEGLKVILRPGPYSCAEWDFGGFPYWLLKDETMRVRTQHAGFMQACERYIN
                     RVGKELSPLQFANGGPIIMVQVENEYGAYGSDKEYIGRLRDCLRNAGFDKTMFFTCDA
                     PRRLTNDVRDDIFCVANFGQNPKAGLEVLRKVRPSGPLMCGEFYPGWFDSWGLKHHVR
                     SAEETIVDLQYMLERRISFSLYMAHGGTSFGFAPGALLDKGYYAPNITSYDYDAPINE
                     AGWDTPKYHALRELYSRYLEPGETLPPIPARKPVITLPEIRLTETAPLLTSATACIDS
                     PLPRPFELYSQSHGCVLYTTKLPPGPAGTLAFPAINDWARIYVDGQAVSTYDRRLVAE
                     ADPVEPSQLEIAGFEDAKKEKEGAAAQKFTLAVPARSSEVELSILVEALGRIHYSKAM
                     GSNYKGLLGAVQLDGRELTGWQAHLLPLTAPHLAALKFSKAPASGPSFYRAQFTLDKT
                     GDTFLDMRNWTKGVVWVNGHNLGRYWNVGPSQTLYLPAPWLKTGQNEIIVLDLFEPKK
                     TAVQGLAEPILDQPAP"
     gene            3958..5253
                     /locus_tag="PKI68_06850"
     CDS             3958..5253
                     /locus_tag="PKI68_06850"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF005301.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNX53447.1"
                     /translation="MAKIIPIDTLGECKIDSPFKAKKQKFFCDDNGVICNVHTSDYAP
                     DFPVDEIEYFQRAGAREKIYFKPGEVIVGVVTCGGLCPGLNDVIRAVTFCALEGYGVK
                     KVLGFQYGYEGLVAKYFHPPIELNTDNTDGIHDKGGTILKSSRGPQDVKEMVHTLRHY
                     KVNILITIGGDGTLRGARAIQEEVTAQGLNIAVIGVPKTIDNDIGNIERSFGYETAVQ
                     SAWEVINCAHAEAKAYRHGVGLVKLMGRESGWIAASTATSNSNVNFCLIPEVPFDLDG
                     PNGFFEHLRRRLEKKDHAVVVVAEGAGQHLIPHADGKDKSGNKKLADIGTFLRDEMNR
                     WFAEKKCELNVKYFDPSYSIRSTPANANDSEYCLFLGYNAMHAAMAGKTGMVVGLHNH
                     RLVHLPVSMTGQRKLVDPHGWIWQAVLQTTRQPVSMVNE"
     gene            complement(5254..6744)
                     /locus_tag="PKI68_06855"
     CDS             complement(5254..6744)
                     /locus_tag="PKI68_06855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNX53448.1"
                     /translation="MKTVSAAEMRELERRVFAGGTAGEELMLRAGQAVSGIAQDVLAG
                     RGGHSILLIAGTGNNGGDIFAAATALSTTDLDIEVWICGSQSRIKGDAQLHLKKMVRA
                     GIIPKEIHSTNDLFPKTWPDLIVDGLLGTGSNGAPRGFMGSLIEWINAEALHAFVLSV
                     DLPSGIDADSGIAAGPAVKADLTVTIGLPKTGLIRPEALPYTGSVEVADIGIPQEFID
                     DAAGCEEAALIDRSDICLPRRERDSHKGTYGHVLLIGGSAGYTGAIAMAARAALRSGA
                     GLVSVLTPEEVWPVVAQAVGPEVMVHPFTRTTPPDLSRFSAVLAGPGLGRSDETRRLV
                     EALLETCGVPLVLDADALCVSPEKITGAKCPVILTPHPGEFERFFGEPVTGRWAQARE
                     AAGTTGKTIVLKGAGTIVAEPGHKLAVNLTGNPGMATGGSGDVLAGLLTGLLGQGMQT
                     FEAAMTAVYLHGIAGDIAAYDCTQSALIATDIIEALPDAFRFLQSR"
BASE COUNT         1408 a         1971 c         2092 g         1483 t
ORIGIN      
        1 ttcgaacgca gccttcgctt gatgcgagat ctgcacggct tcggcgcgtg attcatccgt
       61 tgtattccag tgccggtcga tgacggcctg gatcgcggga tatttcctgt cggtttcatc
      121 atactggttg cgaagttcaa gcagctgctg tttgaggtcc gcgatgacgg gcttgcaggc
      181 cggatcgtcg tagaggttgt tcatctcggt ggggtctttt tccaggtcat agaactccca
      241 gcccggcggg gtttgaattc cggtgccgtc attcttgagg ccgtagaaga agatcagttt
      301 gtagcgatcg gtgcgtatgc cgaagtgggc cgggttccaa tggtgggcca gatgcatcca
      361 gtatcggtaa taggtggctt tgcgccagtc gtccggggtt ttgccggttt ccaggatcgg
      421 ccggatgctg cggccatgca tgtatgccgg gggttttact ccggcgaagt cgagaatcgt
      481 cggggcgaag tccgtgttat taatcagcgc gctgctgcgg gtgccggctg ggacgacgcc
      541 ggggaagcgc acgatcatgg gcatgcgcat ggattcttcg tacatccagc gcttgtcgat
      601 gtaatcatgt tcgcccagcc acatgccttg atcgctggtg tagataatca gcgtattgtc
      661 caagatgcct tcctgtttaa ggtagtcgac cactcgctga acattgtcgt cgacaccctt
      721 cacgcagcgg agatatttcc ggatgtaacg ctgatagctt tcatggagga ttttttcgtc
      781 atccatgtcg gtgttgtttt tcagtcctgc aagcggcagc tgattgcgga agacgttgcg
      841 tctgccgatc gaggctccga tgtagcggac gagttcatcc tgatccccgc gcgtggcgat
      901 ggagccgtgg tttccggcgt tggtcaggct gtcgggttcg gggaaggtga catccgccag
      961 atagttctca tagcgcggag catagtcgaa gtcgtcgtgt ggcgctttat tgtgcaggca
     1021 gaagaagaac ggcttgctcc ggtcacggtt tttcagccaa tccagtgcgg catcggtgat
     1081 gatatccgtg acgtagcctt tgatctgtat cggttgtttt tcgaaagccc ccacaccttt
     1141 tttgaaaaac ttcgggtcga agtattcgcc ttgtccgggc agaaccatgt agtagtcaaa
     1201 attcggctca acatggccga ggtgcatttt cccgatcagc gcggtgttgt agccggcttc
     1261 gtgcatcagc tggggcaggt actggtggtc tgtcgccaga ctgccggtca gatccagaac
     1321 gccgttcgcc tgactgtact gtccggtcag gatggaggcc cggctgggcg tgcagattga
     1381 attgttgcag aagacccggt ccagccgcat gccttcgcgg gccagccggt cgagggccgg
     1441 tgtcgggttt agtgccgcaa aacgaccgtc gtatgcgcca attgcctgag ccgtatgatc
     1501 gtctgacatg atgaataaaa tgttaggctt cgtcggcggt gtcgaagcgg cctcggacga
     1561 gccgagacac agccccagaa tcgatagaga aagacctttg acagatgttt tcatggtcgg
     1621 actctatcgt gttcctgtcg aacgcgccac tgttcaattg ctcatttttc cgccgcggaa
     1681 cggaccgtat gcaccaggtc ttgaaggggc ggggcgcttt ctctatagtc cggtactttt
     1741 tccttaaaac agattcaggt gaaacaatgg cggcggcctt ctgcgcactg gaagggtgcg
     1801 gcggaaaaaa gccactgtgc gcctcgtaac ttgtgagcga tcaggagttg tttgattcga
     1861 ggcggggtag gacgatccgg tgtcgtgccg gtgattggta gattgtaaac aagaggggag
     1921 ttatccatgg cgaatcgtct caagcggttg atcgggctta gtctgttcct gctgattgca
     1981 acattccgga cgggttgtgc cgaaacggcc ggaacctttc aggccggcga gtcggatttc
     2041 ctgctgaatg gaaagccgtt tgtgatccgg tcgggcgagc tgcattactc ccgcgttccc
     2101 cgtgaatact ggcagcaccg gctgcagatg tgcaaggcga tgggcttcaa cacggtgtgc
     2161 gcctatatgt tctggagtgt gcacgagccg gagccgggtc agttcgactt ttccggaccg
     2221 gcggatgtgg ccgccttctg ccgtctggcc cagcaggaag gcttgaaggt gattctgcgc
     2281 cccggcccgt attcgtgtgc cgagtgggat ttcggcggct ttccgtactg gctgctcaag
     2341 gacgaaacga tgcgggtccg tacgcagcac gccggcttca tgcaggcctg tgaacgctac
     2401 atcaaccggg tcgggaagga actctctccg ctgcagtttg ccaacggcgg accgatcatc
     2461 atggtgcagg tcgaaaacga gtatggcgcc tacggctcgg ataaagaata tataggccgg
     2521 ctgcgcgact gcttgcgcaa cgccggcttc gacaagacga tgttcttcac ctgcgatgcg
     2581 ccccggcggc tgaccaacga cgtccgcgac gacatctttt gcgtggccaa tttcggccag
     2641 aaccccaaag ccgggctgga agtcctgcgc aaagtccgcc cctccggtcc gctcatgtgc
     2701 ggcgaattct atccggggtg gttcgattcc tggggcctga aacatcatgt gcgctccgcc
     2761 gaggagacca tcgtggactt gcagtacatg ctcgaacggc ggatctcgtt cagcctttac
     2821 atggcgcacg gcggaacgtc gttcggcttc gcgcccggcg cgctgctgga caaagggtat
     2881 tatgcgccca acatcacctc gtacgattat gacgccccga tcaacgaggc gggctgggac
     2941 acgcccaaat accacgccct tcgcgaactc tacagccgct atctggaacc cggcgaaacc
     3001 ctgccgccca ttccggcccg caaaccggtc atcacgctcc ccgaaatccg gctgaccgaa
     3061 accgctccgc tgctgacctc cgccacggcc tgtatcgact cgccgttgcc gcgcccgttc
     3121 gagctgtaca gtcagagtca tggctgtgtg ctctacacca cgaaactgcc gcccggaccg
     3181 gcgggaaccc tggcgttccc cgcgatcaac gactgggcca gaatctatgt cgacggccag
     3241 gccgtctcga cctatgaccg gcggctggtt gccgaagccg atccggtcga gcccagtcag
     3301 ctggaaatcg ccgggttcga agatgccaag aaagagaaag agggggccgc ggcgcagaaa
     3361 ttcacgctgg cggttccggc ccggagctcg gaggtcgagc tgagtattct ggtcgaagca
     3421 ttgggccgca tccattactc caaggccatg gggtcgaact acaaaggact gctgggcgcc
     3481 gtgcagctgg acggccgcga attaaccggc tggcaggcgc acctgctgcc gctcactgca
     3541 ccgcatctcg ccgcgctgaa gttttcgaaa gccccggcct ccggtccgtc gttctaccgc
     3601 gcgcagttca cgttggataa aaccggcgac accttcctgg atatgcgcaa ctggacgaaa
     3661 ggcgtggtgt gggtcaacgg ccacaacctg ggccgctact ggaatgtcgg cccgtcccag
     3721 acgctgtatc ttcccgcgcc gtggctcaag accggtcaga acgagatcat cgttctggat
     3781 ctgtttgagc cgaaaaaaac agccgttcag gggttggccg agccgattct cgatcagccg
     3841 gccccataaa atcctgcaca gcgctgcatc cgatccggca gaacgcgctc ttgcggagcc
     3901 ggaacggatg cactatattc cgccgctctg aatcagacgc agagaagcag gtgcactatg
     3961 gcaaaaatta ttccgattga tacgctgggc gaatgcaaaa ttgattcgcc gttcaaggcg
     4021 aagaaacaga agtttttttg cgacgacaac ggtgtgatct gcaacgtgca cacgtctgac
     4081 tacgcaccgg attttccggt cgatgaaatt gaatatttcc agcgcgccgg cgcgcgagag
     4141 aagatttatt tcaaaccggg agaagtcatt gtcggtgtgg tgacctgcgg cgggctgtgc
     4201 cccgggctta acgacgtgat ccgtgcggtg accttctgcg cactggaagg gtacggcgtt
     4261 aaaaaggtgc tgggcttcca gtacggttac gaagggctgg tcgccaagta ctttcatccg
     4321 ccgattgaac tgaatacgga caacaccgat gggattcacg acaagggcgg caccatcctc
     4381 aaatcgtctc gaggcccgca ggatgtcaag gagatggttc ataccctccg gcactacaag
     4441 gtgaacattc tcatcacgat cggcggcgac ggcaccctgc gcggcgcccg ggccatccag
     4501 gaagaagtta ccgcgcaggg gctgaatatc gcagtcatcg gagtgccgaa aaccatcgac
     4561 aacgatatcg gaaacatcga gcggtctttc ggctacgaga ccgcggtgca aagtgcctgg
     4621 gaagtgatca actgcgctca tgcggaagcc aaggcctacc gccacggagt cggcctggtc
     4681 aaactgatgg gccgtgaatc cggctggatt gccgcgtcga ccgcgacctc taacagtaat
     4741 gttaacttct gcctgattcc ggaagttccg tttgatcttg atggaccgaa cggattcttt
     4801 gagcatctgc gccgccggct cgagaaaaaa gatcacgccg tggtggtggt tgcggaaggc
     4861 gccggacagc acctgattcc gcatgccgac ggcaaagaca aatccggcaa taaaaagctg
     4921 gcggatatcg ggacttttct gcgcgatgaa atgaaccgct ggtttgccga aaaaaaatgc
     4981 gaactcaacg tcaaatactt cgaccccagc tattccattc gcagcacacc ggccaacgcc
     5041 aacgattccg aatactgcct gttcctcggg tacaatgcga tgcacgccgc gatggccggt
     5101 aaaaccggca tggtggtcgg cctgcacaat caccgcctgg tgcatctgcc ggtttcgatg
     5161 accggacagc gcaagctggt tgatccgcac ggatggatct ggcaggcggt tctgcagaca
     5221 acccgacagc ccgtcagcat ggtgaatgaa tagtcagcgc gactgcagga atcgaaaagc
     5281 atcgggcagg gcttcaatga tatccgtggc aatcagcgcg gactgcgtac agtcatacgc
     5341 ggcgatatcg cctgcgatcc catgcaagta aactgcagtc attgccgctt cgaaggtttg
     5401 cataccctga ccgagcagtc cggtcagcag cccggccagc acatcgcctg aacctccggt
     5461 cgccatgccg gggttgccgg tcagattcac cgccagttta tgtcccggtt ctgccacaat
     5521 cgttccggca cctttgagaa caattgtctt tccggttgtt ccggcggctt cgcgcgcctg
     5581 tgcccagcgg ccggtgaccg gttctccaaa aaaccgttca aattcaccgg ggtggggtgt
     5641 caaaatgacc gggcatttgg ccccggtgat tttttccggt gaaacgcaga gcgcatcggc
     5701 gtcgagcacc agcggaaccc cgcaggtttc aagcagagct tcaaccagcc ggcgggtttc
     5761 atcagagcgg cccagccccg gcccggcaag aactgcgctg aagcgcgata gatcgggcgg
     5821 cgtcgtgcgg gtgaaaggat gcaccatcac ttccggtccg acggcctgcg cgacaacggg
     5881 ccagacttct tcgggtgtca gaacgctgac gagacccgcg ccggaacgca gcgccgcacg
     5941 cgcggccatg gcgatagcgc cggtatagcc ggcggatcct ccgatgagca gaacgtgacc
     6001 gtaggttcct ttatggctgt cgcgttcgcg gcgcggcagg cagatgtcgg aacggtcgat
     6061 caaggccgct tcttcgcatc cggcggcatc gtcgataaat tcctgcggaa ttccgatatc
     6121 ggcgacctcg acgcttccgg tatagggaag cgcttcaggg cggatgagac ctgttttagg
     6181 cagtccgatg gtgaccgtaa gatcggcctt caccgccggc ccggcggcga ttccgctgtc
     6241 ggcatcaatg ccggacggaa gatcgaccga aagcacaaac gcgtgcagtg cttcggcatt
     6301 gatccattcg atgagcgaac ccatgaaacc gcgcggtgca ccgtttgatc cggtcccaag
     6361 gaggccgtcc acaatcaaat cgggccatgt tttcggaaag agatcatttg tgctatgaat
     6421 ctctttgggg atgattccgg cgcgcaccat tttttttaga tggagctgtg catcgccttt
     6481 aatccggctt tgcgaaccgc atatccagac ttcaatatcg agatcggttg ttgagagagc
     6541 cgttgcggcg gcgaaaatat cgccgccgtt attgccggtt ccggcgatca gcagaatcga
     6601 gtgtccgccg cgaccggcca ggacgtcctg tgcaatgccg gaaacggcct gccctgcgcg
     6661 cagcatcagt tcttcaccgg ccgtaccgcc cgcaaagacc cggcgttcca gctcgcgcat
     6721 ttcggctgca gaaacggttt ttataggccc gaccttatcg tctgcttcgg gggtgcagaa
     6781 cagtttcccg aatcgtaaac ataaaaaaag ccggaacagc caaggccgtt ccggcttttg
     6841 tcgtcatcag tctatttcgt cagtttgctt ctttttcatc tgacggcgca gtaagaacac
     6901 catcgcgccg gccagaccga acaacccggc cgttgccggt tcaggaatga gctg
//
LOCUS       DAOPZO010000096         6889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_13429_length_6889_cov_8.923032, whole genome shotgun sequence.
ACCESSION   DAOPZO010000096 DAOPZO010000000
VERSION     DAOPZO010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13429_length_6889_cov_8.923032"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..726)
                     /locus_tag="PKI68_06860"
     CDS             complement(1..726)
                     /locus_tag="PKI68_06860"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNX53449.1"
                     /translation="MRFVLYNIRYGTGGKKLHSPLNGYLRRTGKNLDRITQFLQDLNP
                     DVAGLVEVDAGSYRSKKNNQAEMIADRLGHHHVYRSKYGENSLTRHLPILSHQGNAFV
                     ARDSDHKETFHYFDRGVKRLVIELEMENVTFFLIHLALGSRLRHRQLGDLYDLLQTVK
                     KPHIVAGDFNALWGEQEIRLFQAATGLKNAHAGNSPTFPSWNPKRHLDFILHSPGIDV
                     ERCWMPEVCFSDHLPIVCDFTVA"
     gene            complement(723..1952)
                     /locus_tag="PKI68_06865"
     CDS             complement(723..1952)
                     /locus_tag="PKI68_06865"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNX53450.1"
                     /translation="MLSEHRRIHMIGIGGIGMAGLALLLKERGHRVSGCDEQENRQTA
                     WLREKGIPVLIGHSRDHLKDAEWIIRTTAVPDDHPEVKNATVPVSRRGEVLPALLRDR
                     ITIAVSGTHGKTTTTAMIAQILGCGYCIGGEVPGFDGVAKDGEVMVVEADESDGTVAN
                     YHPDIAVITNIEYDHMEHHASVEAFEACFKKFIANTKQKIVYCAEDPAAARLCTGNPK
                     AVSYRFSPLRIALSGRHNQLNACAALEVAKQWKSEAAIFAALEKIKPVRRRFEKVFQG
                     DCTIISDYAHHPTEIRTLVQTALETLKPKRLLGVFQPHRYTRTKALMADFPPAFAGID
                     KLWLLPVYAASEKPLAGGTTADLFRNFPPGGKPDVENCESLDAAWLDIQRELRAGDLL
                     LIIGAGDIEQLAERARR"
     gene            complement(1958..3031)
                     /locus_tag="PKI68_06870"
     CDS             complement(1958..3031)
                     /locus_tag="PKI68_06870"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HNX53451.1"
                     /translation="MSENLHIAIACGGTGGHIFPGLATARVLRDRGHHVTLWLAGKGG
                     ENTAVQGWEGRIVTIPSEGFQFGAFRSVLTAIRITGAVLRCWFIMLRHKPDVVLAMGS
                     YASIGPCFAARLHGIPYILHEANAVPGRAVRLLAPKARAIAICFEETRYHLKGMNAVS
                     AGMPLRSEIQRTENRAPKSGFNLLVMGGSLGAHAINEIVSEAICALKNPAVSVTHLTG
                     PADEPAVAERYRAAGVNAEVHAFTHDMTSLYEKASLAICRSGASTCAELGVFALPALL
                     IPYPHAASDHQTANARALEKFGAADVIRQSDMTAEWLKDYLAAQIEDPSRLEKMSARS
                     AARNVPLNAAAILAGVVEECARK"
     gene            complement(3028..4308)
                     /locus_tag="PKI68_06875"
     CDS             complement(3028..4308)
                     /locus_tag="PKI68_06875"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNX53452.1"
                     /translation="MSTTGLHSAVQSLFARTAHGIKPGLEVTEALLEKLGNPQRGLKC
                     IHVAGTNGKGSVCAMIEAVLRASGFKTGLYTSPHLFRFNERFRINGRDIPDPVMEQLI
                     ADVEAAAQSLDTRPATFFEIATAMAFEYFKREKVDYAVIETGMGGRWDATNVIQPLLS
                     VITRIDFDHAEYLGSEIEKIAGEKAGIIKPGVPVICGPMPVEAEAVIYKEALEKKAPI
                     LGSDDAVFFKLLEKRRDDQLINIEVSGREYPNIRLPLAGSFQLENCGIAVAALEDLAG
                     AENIRLQMKRGLEAVKWPGRFQLIQQKPPVLYDGAHNVCGARALFETLGETFPGFGNG
                     FFFSFMKDKDVEGILKTLEPAVEKAWAFTLPGDRAMKAEEIAEIGKRIGLEITPAATP
                     QPVLDWLNAGKKRLVCFTGSLYLPGELKKYGFFK"
     gene            complement(4305..5150)
                     /gene="accD"
                     /locus_tag="PKI68_06880"
     CDS             complement(4305..5150)
                     /gene="accD"
                     /locus_tag="PKI68_06880"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNX53453.1"
                     /translation="MALRFGKKKYSTVQVRKRDVPDGLWEKCPGCGEILYAKQLEESL
                     QVCSKCSHHFTLGRQKRIEMFADEGTFVEWDADMISADPLGFTGKDSYIAKLESNKKK
                     TGFKDAVTCGAAKLNGRDIGLGVMDFSFLGASMGSVVGEKITRLIERCTAEKRPCILV
                     CASGGARMYEGLYSLMQMAKTSAALARHAKAGLPYIPVLTHPTMAGVMASFATLGDII
                     IAEPGALIGFAGPRVIKETTQQDLPEGFQRAEFLEKHGLIDMVVPRSQLKARVSLILD
                     YMTDK"
     gene            complement(5242..5961)
                     /locus_tag="PKI68_06885"
     CDS             complement(5242..5961)
                     /locus_tag="PKI68_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNX53454.1"
                     /translation="MFSNHYRSMLDLLYPRNCTYCGVPDPDPMKYLCWDCLSDTPKVE
                     PPFCAVCGDPVAGDIQHDYTCFACARETPAFDRARSSVRYEGAVGELLRALKYGNALW
                     VVQDLAGLLSACVHAEYPDQPFDVVTAVPLHPPRLRERGFNQSAVLGAALARRLGLPF
                     RGGLVRRVRPTVTQTGLTAPQRTANVCGAFRVGLFARPAGRRILLVDDVMTTGATVNA
                     CAKALKTGGAASVHVVTVARG"
     gene            6153..6728
                     /locus_tag="PKI68_06890"
     CDS             6153..6728
                     /locus_tag="PKI68_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53455.1"
                     /translation="MIKCIRIMTVTALLGCTAATAQDVKIESVELTEPTTPIYQTEEG
                     VDTIQIKGLMQTAVSGKKWFRISVNYSTDTDWMDRLTLEYYILFPNETNVIKGVINYV
                     DIPKNREHLSEMYMHFNSYARHYKRGTINYAVVALVDGKQVAVETNKRSPENWWKDML
                     VNPCGLLDRSMTPFVVFNVEKFEAQDLCSWK"
BASE COUNT         1532 a         1898 c         1969 g         1490 t
ORIGIN      
        1 ttacgcaacc gtaaaatcgc agacgatcgg caggtgatcg gaaaagcaga cctccggcat
       61 ccagcagcgc tcaacgtcga taccggggct gtgcaggatg aaatcgaggt ggcgcttggg
      121 gttccagctc ggaaacgtcg gcgagtttcc tgcatgcgca ttcttcaaac cggtcgcggc
      181 ctgaaacagg cggatttcct gttcgcccca gagcgcatta aagtcaccgg ccacgatgtg
      241 cggttttttg accgtctgga gcagatcata cagatcgccg agctggcggt ggcgcaggcg
      301 cgaaccgagc gccagatgga tcagaaaaaa ggtgacgttc tccatctcca gttcgatgac
      361 cagccgtttc acgccgcggt cgaaataatg gaaggtttcc ttatggtcac tgtcgcgcgc
      421 cacaaaggca ttgccctgat ggctgaggat cggcaaatgg cgggtcagcg aattttcacc
      481 gtacttggag cggtaaacgt gatgatgccc cagccggtcg gcaatcattt cggcctggtt
      541 gttctttttt gaacggtagg aaccggcatc cacctcgacc agtccggcca cgtcgggatt
      601 cagatcctgc agaaactgcg taatccggtc taaattttta ccggtgcggc gcagatagcc
      661 gttcagcggc gagtgcagct ttttcccgcc cgtaccgtac cggatgttgt agagcacaaa
      721 tctcatcgtc tcgcccgttc tgccagctgt tcgatgtcgc cggcgccgat aatcagcagc
      781 agatcgccgg cgcgcagttc gcgctgaata tccagccagg ctgcgtcgag cgattcacaa
      841 ttttcgacat ccggcttccc gccgggcgga aaattccgga acagatccgc ggtggttccg
      901 cctgccagcg gtttttccga ggcggcataa accggcagca gccacagttt atcaataccg
      961 gcaaacgccg gcgggaaatc cgccatcagc gctttggtgc gggtgtagcg gtgcggctga
     1021 aaaacgccga gcaggcgctt cggcttgagg gtttccagcg cggtctgaac cagcgtacgg
     1081 atttccgtcg gatggtgggc atagtcggaa ataatcgtgc agtcgccctg aaaaaccttt
     1141 tcgaagcgcc gccggaccgg ctttattttt tccaatgcgg caaaaattgc ggcttcggat
     1201 ttccactgct tcgccacttc cagcgcggca caggcgttga gctgattgtg gcgtccggag
     1261 agtgcaatgc gcagcggcga aaatcggtac gacaccgcct tcggattgcc ggtgcagagg
     1321 cgcgccgcgg ccggatcttc ggcgcaatag acgatcttct gcttggtatt ggcgatgaat
     1381 tttttgaagc acgcttcgaa ggcctcgacg ctggcgtggt gctccatgtg gtcgtattca
     1441 atattggtga tgaccgcgat gtcgggatga tagttcgcga cggttccgtc gctctcgtcg
     1501 gcctcgacca ccatgacttc gccgtctttg gccacgccgt caaagccggg cacttcgccg
     1561 ccgatgcagt atccgcagcc gagaatctgc gcgatcatcg ccgttgtcgt ggttttaccg
     1621 tgcgttccgc tgacggcgat ggtgatgcgg tcgcgaagca aagcgggcag cacttcgccg
     1681 cgccggctga ccggaacggt cgcgttcttc acttcaggat gatcgtccgg cacggccgtc
     1741 gtgcgaataa tccactcggc gtccttcaga tgatcccggc tatgcccgat gagcaccgga
     1801 ataccttttt cacgcagcca ggcggtctgg cggttttcct gttcgtcgca gccgctcacg
     1861 cggtgtccgc gttctttcag cagcagcgcg aggccggcca ttccgattcc gccgataccg
     1921 atcatatgaa tacggcggtg ctctgaaagc atattgtcta tttgcgggcg cattcttcca
     1981 ccaccccggc aaggattgcg gccgcattca aaggaacgtt acgcgcggcg gaacgcgcgc
     2041 tcattttttc aagccgggaa ggatcttcga tctgcgcggc cagatagtct ttgagccact
     2101 cagccgtcat atcgctctgc cggattacgt cggcggcacc gaatttttcg agtgcgcggg
     2161 cgttggcggt ctgatgatcg ctggccgcgt gcggatacgg gatcagcagc gcaggcagcg
     2221 caaaaacgcc aagctccgcg caggtagagg cgccggaacg gcagatggcc agcgaggctt
     2281 tttcgtaaag cgatgtcatg tcatgcgtga acgcatgaac ttcggcgttt actccggcag
     2341 cacggtaacg ttcggctacg gccggttcat ctgccggacc ggtcaaatgc gtaacgctta
     2401 ccgccgggtt cttcagcgca caaatcgcct cggaaacaat ttcattaatc gcgtgagcgc
     2461 ccagcgagcc gcccataacg agcagattaa atccgctttt cggtgctctg ttttccgtcc
     2521 gctgaatttc cgaacgcaac ggcattccgg cggaaaccgc attcattccc ttgagatgat
     2581 aacgggtttc ttcaaaacag atggcaatgg cccgcgcttt cggagccagc agccggacgg
     2641 cccggcccgg caccgcgttg gcttcgtgca gaatgtacgg aatgccgtgc aggcgtgcgg
     2701 cgaaacacgg accgatcgat gcgtaactgc ccatggccag cacgacatcc ggtttgtggc
     2761 ggagcatgat aaaccagcag cgaagcaccg cacccgtaat ccggattgca gtcagcacag
     2821 accggaatgc gccgaactgg aatccttccg aaggaatggt gacaatccgc ccttcccagc
     2881 cctgtacagc ggtgttttcg ccgccttttc cggcgagcca gagggtgacg tgatgtccgc
     2941 ggtctctcag gacgcgggcg gtggcgaggc cgggaaagat gtggccgccg gtgccgccgc
     3001 aggcgatggc gatatgcagg ttttcactca tttgaaaaat ccgtattttt taagttctcc
     3061 gggaagatac agtgaaccgg taaagcagac cagccgcttt ttaccggcat tgagccaatc
     3121 gaggacgggt tgcggcgttg cggccggcgt aatttccagc ccgatccgtt tgccgatctc
     3181 cgcaatttcc tccgctttca tcgcgcggtc gccgggcagc gtaaacgccc aggctttttc
     3241 aacggcgggt tcaagcgttt tgaggattcc ctcgacatcc ttgtccttca tgaagctgaa
     3301 gaaaaagccg tttccgaatc ccggaaaggt ttcgcccaga gtttcaaaaa gcgcacgggc
     3361 accgcagacg ttgtgcgcgc cgtcataaag taccggcggt ttctgctgaa tcagctgaaa
     3421 gcgccccggc catttaacgg cttcgagtcc ccgtttcatt tgaagacgga tattctccgc
     3481 cccggcaagg tcttcgagcg cggcgacagc tatgccgcag ttttcgagct ggaagctgcc
     3541 cgcgagcggc agacggatat tcgggtattc gcgtccgctg acttcgatat taatgagctg
     3601 gtcgtcgcgc cgtttctcca gcagtttgaa aaacaccgca tcgtcgcttc cgagaatcgg
     3661 agcttttttc tccagcgctt ctttatagat aacagcttcc gcctcgaccg gcatcgggcc
     3721 gcagatgacc ggtacgcccg gcttgatgat gccggctttt tcaccggcaa tcttttcgat
     3781 ttcgctgccg agatattcag catggtcgaa gtcaatgcgg gtgatcactg agagcagcgg
     3841 ctgaatgaca ttcgtggcgt cccagcgtcc gcccatgccg gtttcgatga cggcgtagtc
     3901 caccttttcg cgtttgaaat attcaaaggc catcgccgtg gcgatttcaa agaaagtcgc
     3961 cggacgcgtg tcgagacttt gcgcagcggc ttcgacatcg gcaatgagct gttccatcac
     4021 cggatcggga atatcacgcc cgttgatgcg gaagcgttcg ttgaagcgaa aaaggtgcgg
     4081 cgaggtgtag agaccggtct taaaaccgga agcgcgcagc accgcttcga tcatggcgca
     4141 gaccgagccc ttgccgttgg ttccggcaac gtggatacat ttcaggccgc gctgcggatt
     4201 gccgagtttt tcaagcaggg cttcggtaac ttcaagaccg ggcttgatgc cgtgcgctgt
     4261 gcgggcaaac agggattgaa ccgcggagtg aagaccggtt gtgctcattt gtccgtcata
     4321 tagtcgagga tcagagaaac gcgggcttta agctggctgc ggggaacgac catgtcgatg
     4381 agtccgtgtt tttcgagaaa ctcggcgcgc tggaagcctt ccggcagatc ctgctgcgtc
     4441 gtttctttga tgacgcgcgg accggcaaag ccgatgagcg cgccgggctc ggcgatgata
     4501 atatcgccca gcgtggcgaa gctggccatg acaccggcca tggtcggatg cgtaagcacc
     4561 ggaatatacg gcaggccggc tttggcgtgg cgggccagcg ctgcgctggt tttcgccatc
     4621 tgcatcagac tgtaaagacc ttcgtacatg cgggctccgc cggaggcgca aaccagaatg
     4681 caggggcgct tttcggccgt gcagcgttcg atgagacggg ttattttttc gccgacgacg
     4741 ctgcccatgc tggcaccgag gaaagagaag tccatcacac cgagaccgat gtcacgtccg
     4801 ttgagcttag ctgcgccgca cgtgacggcg tccttgaatc cggttttctt cttattggat
     4861 tccagtttgg cgatgtagga atctttcccg gtaaaaccga gcgggtcggc ggaaatcata
     4921 tcggcgtccc actcaacgaa cgtgccttca tcggcaaaca tttcgatgcg tttctgccgt
     4981 ccgagggtga agtggtggct gcacttggaa cagacctgaa ggctttcttc gagctgcttg
     5041 gcataaagga tttctccgca gccggggcat ttttcccaaa gaccgtccgg aacgtcccgt
     5101 ttgcggacct gaactgtcga atattttttc ttaccgaatc gtaaagccat agaaattcct
     5161 tggattactg gagtgccgga aacattggag cattgggaaa tccgtattcc gctgctccgt
     5221 catccaatca ctccatcttt cttatcctct cgcaacagtt accacatgca ccgatgccgc
     5281 cccgcccgtt ttgagcgctt tggcacaagc gttgaccgtg gcaccggtgg tcattacatc
     5341 atcaaccagt aaaattctcc ggcccgccgg acgggcaaaa agcccaaccc gaaaagcgcc
     5401 gcacacgtta gctgtccgct ggggggctgt caaacctgtt tgggtcaccg tcgggcgaac
     5461 gcgccggaca agcccccctc tgaacggaag ccccagccgc cgggcgagcg cagcacccag
     5521 cacagctgac tggttaaagc ctcgttcacg caggcgcgga ggatgaagcg gcactgctgt
     5581 caccacatca aacggctggt cagggtattc agcatgcaca caggcagaaa gcagtccggc
     5641 aagatcctgt acgacccata aagcatttcc atacttgaga gcacgcagaa gttcgccgac
     5701 agcaccttca taacggactg aagaacgggc gcggtcgaaa gccggggttt ctcgggcaca
     5761 ggcaaagcag gtatagtcgt gctgaatatc gcctgcaacc ggatcgccgc aaacagcaca
     5821 gaacggaggt tcgactttcg gtgtgtcaga aagacaatcc cagcaaagat atttcatcgg
     5881 gtcgggatcc ggcactccgc agtaagtaca gttgcgagga taaagcaggt cgagcattga
     5941 gcgataatgg tttgaaaaca cagagcaccg gaagtaaaaa acataaaaaa aagctgatcc
     6001 aggcgcgagc tgtcttcgta ttcataaaga ggaaaaccgg tttttcagaa taaaaacagg
     6061 cggtttaatt gatgctgctg tgatcacccg ttatattgca aaggttagtt gtattccaaa
     6121 tgtagtttta ttcaaaaaca ggaggaggca ttatgataaa gtgtattcgg attatgacgg
     6181 taaccgccct tttgggatgc accgccgcaa cggcccagga tgtcaaaata gaatccgttg
     6241 agctcaccga acctaccacc cccatctacc agactgaaga aggagtggat accattcaga
     6301 ttaaaggact catgcagaca gccgtctctg ggaaaaaatg gttccggatt tctgtaaact
     6361 actcgaccga tacggactgg atggatcggc tgacacttga atactacatc cttttcccga
     6421 acgagacaaa cgtgataaag ggcgtgatta attatgtaga catcccgaaa aatcgcgagc
     6481 atctcagcga gatgtatatg cacttcaaca gctatgcgcg ccactacaag cggggcacca
     6541 ttaattacgc agtcgtcgct ttggtagacg gcaaacaggt agccgtcgaa acaaacaaac
     6601 gctcgccgga aaactggtgg aaagatatgc tagtcaaccc atgcggactg ctggatcgaa
     6661 gcatgacgcc gtttgtagtt ttcaatgtgg aaaaatttga agcacaggat ctctgctcct
     6721 ggaaataaaa gcggatgaag aaaaaagagt gccggaaacg gcactctttt tttgtcgatt
     6781 taagggcaag aacacataaa agccaaggat cactggaagc aaggggtttg acaggctctt
     6841 acggcataaa aagccgttta tatccgctaa acggaaagac gtaatcaga
//
LOCUS       DAOPZO010000097         6801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_13643_length_6801_cov_8.964423, whole genome shotgun sequence.
ACCESSION   DAOPZO010000097 DAOPZO010000000
VERSION     DAOPZO010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13643_length_6801_cov_8.964423"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PKI68_06895"
     CDS             complement(<1..567)
                     /locus_tag="PKI68_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53456.1"
                     /translation="MKRMMIWLAAASTVFTAQSEIVNGNFTAGEAGRIVNLRSLSQID
                     KGWYANDFSVQHFKFINGAVTLNSSEVTSRPAMIGQFFTYNGTGERVLKFDTAVTDSN
                     SNLDFRVQLYGYKQRTAEKTILFVNAIKLENTDHPVTSEYYEVKELVNCTHLHAGTLN
                     NGNVTTQSVTFAASADYAFYGIRIIANRP"
     gene            749..2020
                     /gene="murD"
                     /locus_tag="PKI68_06900"
     CDS             749..2020
                     /gene="murD"
                     /locus_tag="PKI68_06900"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNX53457.1"
                     /translation="MHKYSKALVVGCGRSGRAAEALLRSEGASVVSICQEITPDYRYE
                     DLHFDPDVAVVSPGFSLKHPWIKDLIERGVPLLSELELGWSRRHCPIIAVTGSNGKST
                     VVKWIVDALAQAGQKAVPCGNYGFPVCGAVMLHDVPDWLVIEVSSFQLETVQEFRPDI
                     GLLLNVLPNHLDRHGNMDTYRDLKLRLFENMTETDTAVVPSELALSIGKKLPKHLKSF
                     GTEPGADFHYQAGRVGDINLSGTRFANEVLGAAGAAVAAVCSACGIPPSAVEASARVF
                     EPLPHRTALVAEIDGVKYVDDSKATNLVAMCAAVRMCSGKIHLIAGGRPKETDFSLAK
                     DLLAQRVSHLYLIGEASGMMQAAWGDAVRCSSCGTLEKAVMAARKQARPGDTVLLSPA
                     CTSFDQFRDFNERGDCFAAAVRGLTGNDANV"
     gene            2052..2987
                     /locus_tag="PKI68_06905"
     CDS             2052..2987
                     /locus_tag="PKI68_06905"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNX53458.1"
                     /translation="MKAKVAGLPILAIHIMVLAGFSLMQGCKTAKVADVPQDQPVVLP
                     ADDLAGDKAPVLPPVSLPGPSSKTYSVQKGDTLSSIASAHGTTWKKLAELNNLSNPNQ
                     LKVGQEIRISESAGAASSSSASGKHAAASVSSRPIPGSIKQGSSYVIQKGDSLSSIAK
                     RSGLTVSEIKAANSLSRDSLVVGKSLTIPKKGAVKAASVAKAAPAKAANPASAKAAVA
                     PAALPAPAPLADAASTGSVAAVAPAAPAVPAAAAPVAKAAGAPVYEHVLYPGETLDDV
                     ARQYGSSQQEIMKLNNITDAASIKPGTKLLVPIPE"
     assembly_gap    3246..3345
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3346..4077
                     /locus_tag="PKI68_06910"
     CDS             <3346..4077
                     /locus_tag="PKI68_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNX53459.1"
                     /translation="KRGLLPPVAGLGFCAMLLMAEPDFGTTVLVALVIISLLYIGGAR
                     ALHLFGFTAAGAVALAVLLAHNENRMRRIMAFLDPEKYAQGEAWQLINGLNAFASGGP
                     YGVGLGNSIQKYHYLPEAHTDFILPIIGEELGLIATLVVLILFVVVFFCGLRISSRAS
                     DDFGRFTALGITMMITFQAMINLAVVTGSMPTKGLALPFISYGGSSLLVSSMMVGLLI
                     NVAHTAIDPKVKTRTALFKDRQHTA"
     gene            complement(4080..5018)
                     /locus_tag="PKI68_06915"
     CDS             complement(4080..5018)
                     /locus_tag="PKI68_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNX53460.1"
                     /translation="MADVDYQQLEQRLGRTLLAQRLKIQTHHVARLFGSGKVWFHIEH
                     IRTLHRIIRNLFKVTGLYWRGHRNALTVRVNFQELPCPDLPEAFDGFRLLHISDTHID
                     GNPELIDTLIRLIQPLEYDLCVITGDFREGTFEDYDTPAHRMVGLRRHIKTATYAILG
                     NHDAIEMVPALEKGGIRLLLNEHVALERGGTTIWLAGVDDPHYYETDNLDKALDGVPE
                     ESFKILLAHSPEIFRKAAFAGIHLYLCGHTHAGQICLPGGRPLLTNSKCPRAYHAGLW
                     HFEGMTGYTSRGAGTSSVAVRFNCPAEITVHTLRRK"
     gene            5069..>6801
                     /locus_tag="PKI68_06920"
     CDS             5069..>6801
                     /locus_tag="PKI68_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX53461.1"
                     /translation="MNTARKKEESRRLDGQIERTVFRNEENGYAVLRVKVRGHKDLVT
                     VVGTVSSANPGEWLAADGEWITDAHYGRQFKAETMRISQPDTLDGIKKFLGSGMIRGI
                     GKEYAERLVQAFGRDVFDVIENSSAKLLKVEGIGPGRRASIRAAWAEQKGIREIMSFL
                     FSHGVSTARAFRIYKAYGERSIELIQRDPYCLARDIHGIGFLIADGIAQKLGIAKDSD
                     LRARAGIEYVLQELTTNGHCAAPRGDLQSKTVELLDIPPDRISGALDWLIAAKRLILD
                     EDRQGRALVYLPKLYFAEWAFAKKLTELNYGKHPCPQIDFEKALEWVQKKTKIQLSEN
                     QCEALRLAVQAKVMVITGGPGVGKTTLVNSIIQVLVAKKLAVSLCAPTGRAAKRLSET
                     TGLEAKTIHRLLQFNPGTGGFRHTEDNPLEGDVFIIDEASMIDQVLAYQLLQAVPQRA
                     AVIFVGDSDQLPSVGPGRVLCDIIASGAVPVVALTEIFRQAAESRIVTGAHRINHGQL
                     PEFPEEGDKTSDLYFVEAEEAQKVIGVISKMVSESIPQRLGFNPVEDIQILTPMQRGE
                     LGARNLNQVMQG"
BASE COUNT         1366 a         1838 c         1987 g         1510 t
ORIGIN      
        1 cgggcggttc gcgatgatgc ggatgccgta gaacgcgtag tctgcgcttg cggcgaatgt
       61 gacgctttgt gttgttacgt ttccgttgtt tagtgttccg gcgtgaaggt gtgtgcagtt
      121 aaccagctct ttaacttcgt aatattccga ggtgaccgga tggtcggtgt tttcaagttt
      181 gattgcgtta acgaacagaa tggttttttc ggcggtgcgc tgtttgtagc cgtagagctg
      241 cacccggaag tcgagattgg aattgctgtc tgtaacggcc gtatcaaatt ttaagacacg
      301 ctcgccggtg ccgttgtaag tgaaaaactg tccgatcatg gccggcctgg acgtgacttc
      361 cgaactgttc agcgtaacgg cgccgttgat gaatttaaaa tgctgaactg agaagtcgtt
      421 ggcataccag cctttgtcga tctgggagag cgatcgtaag ttgacaattc tgccggcttc
      481 gcccgcggtg aagttcccat tgacgatttc ggactgagct gtaaaaaccg ttgatgccgc
      541 cgccagccat atcatcatcc ttttcatacg tctgctcctg ccccggtatt tttgatgttc
      601 cgccgtaaaa aacggacgca gtgtaagccg gcccgtgaaa acagtaaatt taaaactccg
      661 ccgttccctg tgacattgcg gaaaagagcg gcaagaaacg atgaactatt agatagacag
      721 cggccgcggc agttctaata tctgcgcaat gcataaatac agcaaagcac tggttgtcgg
      781 gtgcggacgc agcgggcgcg ctgccgaagc gctgctgcgc tccgaaggcg cgtcggtggt
      841 cagtatttgt caggaaatca cgccggatta ccgctatgaa gaccttcatt ttgatccgga
      901 tgtcgccgtc gtcagtccag gcttttctct gaagcatccg tggataaagg atttaattga
      961 acgcggggtt cctctgcttt ccgagctgga gctgggctgg tcgcgccgcc actgtccgat
     1021 catcgccgtg accggctcca acggcaagag cacggttgtt aaatggatcg tcgatgcact
     1081 ggcgcaggcc gggcagaaag cggttccgtg cggaaactat ggttttcctg tgtgcggtgc
     1141 ggtgatgctt catgatgtgc cggactggct ggtgatcgaa gtcagctcgt ttcagctcga
     1201 aacggtgcag gaattccgtc cggatatcgg cctgctgctc aatgtcctgc cgaatcacct
     1261 cgatcgccat ggaaatatgg atacctaccg cgatctcaaa ttgcgcctgt ttgaaaatat
     1321 gacggaaacc gacacggcgg ttgttccttc tgagctggct ctcagtatcg ggaaaaaact
     1381 gccgaaacat ttgaaaagct ttggcacgga gccgggggct gatttccatt atcaggccgg
     1441 ccgggtcgga gatatcaatc tgagcggtac ccggtttgcc aatgaagttc tcggcgcggc
     1501 gggtgcggca gtcgccgccg tttgctctgc ctgcggtatt ccgccgtcgg cggtagaggc
     1561 cagtgcgcgg gtttttgagc cgcttccgca ccggacggcg ctggtggcgg agatcgacgg
     1621 ggtcaagtat gtggatgatt ccaaggccac aaatcttgtt gcgatgtgcg cggccgtcag
     1681 aatgtgctca ggaaaaatcc acttaatcgc cggcgggcgg cccaaagaga ccgatttcag
     1741 tttggcaaaa gatttactgg cgcagcgggt ttcgcatcta tatcttatcg gggaagcatc
     1801 gggtatgatg caggctgcgt ggggggacgc cgtccggtgt agttcctgtg gaacgttaga
     1861 aaaggctgtg atggccgcac gaaaacaggc aagaccgggt gataccgttc ttctatcccc
     1921 tgcgtgtacg agttttgatc agtttcgcga ttttaacgag cgcggcgatt gtttcgccgc
     1981 tgctgttcgc ggtctgaccg ggaatgatgc aaatgtataa acgggtaagg tcacaagaga
     2041 aggagttgtt catgaaggcg aaggttgcag ggttaccgat tctggcgata catattatgg
     2101 ttctggccgg gttttctttg atgcagggtt gtaagacggc gaaagtcgcc gatgttccgc
     2161 aggatcagcc ggtggttctt cctgccgacg atctcgcagg cgataaagca cctgttcttc
     2221 ctccggtaag tctgccgggt ccgagttcaa aaacctacag tgtgcaaaag ggcgataccc
     2281 tctcatcgat tgcctcggca catggcacta cttggaaaaa gctggccgaa ctgaataacc
     2341 tttccaatcc caatcaattg aaagtaggac aggaaattcg tatttctgaa tcagccggcg
     2401 cagcttcctc atcatccgca tccggcaaac acgccgccgc atccgtctcc agccgtccga
     2461 tccccggttc gatcaaacag ggttcttcct atgttattca aaaaggcgat tcactttcga
     2521 gcattgccaa acgcagcggg ctgactgtca gcgaaatcaa ggcggccaac agtctttccc
     2581 gcgacagtct tgttgtcgga aagagcctta cgattccgaa gaaaggtgcc gttaaagcgg
     2641 cttctgtagc gaaggccgct cctgctaagg ccgccaatcc tgcgtctgcc aaggctgccg
     2701 ttgcgcctgc cgcactgcct gctccggctc cgctggccga cgccgcttca accggttctg
     2761 ttgccgctgt tgcgcctgcc gctccggctg ttccggccgc tgctgctccg gtcgctaaag
     2821 ctgccggcgc tccggtttac gaacatgtgc tttatcccgg cgaaacgctg gatgacgtag
     2881 cccgtcagta tggcagctcg cagcaggaaa tcatgaagct gaacaacatc acagatgctg
     2941 cttcgatcaa gcccggcacg aagctgctgg ttccgattcc ggaataatgg ttcatgcagt
     3001 tgcaaaaagg gacgctttta ccggcgtccc tttttttgtt ctcaatacga aagcttcatg
     3061 gaatagttca gttatgaaac gaaccatttt tgtcctgatt ggcgcagtac tcattctggc
     3121 gaccatcggt ctggttatgc tgttcagcgc cagcatggtg cgtggcgatt cccagtataa
     3181 taattccgcc tattttgtga cccgccagct tttctggatg gcgatttctc tgattgccgg
     3241 cattgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaaacg cgggctgctt
     3361 ccgccggtcg ccggacttgg tttttgcgct atgctgttga tggcagaacc ggatttcggc
     3421 accacggttc tggttgctct ggtgatcata tcgttactgt acatcggcgg ggcccgtgcg
     3481 cttcaccttt ttggatttac tgccgccggc gccgttgcac tggcagtgct tttggcgcat
     3541 aacgaaaacc gcatgcggcg cattatggcg ttccttgatc cggaaaaata tgcgcagggc
     3601 gaagcgtggc agcttatcaa cggcctcaac gcattcgctt ccggcgggcc gtacggcgtc
     3661 ggtctgggca acagcattca gaaatatcat taccttccgg aagcgcacac cgactttatt
     3721 ctgccgatta tcggcgagga attaggtctg attgccacgc tggttgtgct gattctcttt
     3781 gtggtcgttt ttttctgcgg actccggatt tcctcgcgcg cttcggatga tttcgggcgg
     3841 tttaccgcgc tgggaatcac gatgatgatt acatttcagg cgatgattaa tctggctgtc
     3901 gtcaccggct caatgccgac gaagggcctg gctctcccat tcatcagcta cggcggatcg
     3961 agtctgctcg tttcgtcgat gatggtcggt ctgctcatca acgtggcgca cacggcgatt
     4021 gatcccaaag tcaaaacccg cacggcgctg ttcaaagacc gccagcacac agcataagac
     4081 tatttcctgc gcaatgtgtg tacggtgatt tccgccgggc agttaaagcg tactgccacc
     4141 gacgaggttc cggcgccgcg cgaggtgtag ccggtcattc cctcaaaatg ccacagcccg
     4201 gcgtgatagg ctcgcggaca tttgctgttg gtgagcaggg gccgtccgcc cggcaggcag
     4261 atctgtccgg catgtgtgtg gccgcacaga tagagatgaa tgccggcaaa cgcggctttc
     4321 cggaagattt ccggcgagtg cgccagcagg attttaaaac tctcttccgg cacgccgtcg
     4381 agcgccttgt cgagattgtc ggtttcgtag tagtgcgggt cgtccacgcc ggccagccaa
     4441 atcgttgtgc cgccgcgttc cagcgcaacg tgttcattga gcagcagccg gatgccgcct
     4501 ttttccagcg ccggaaccat ttcaatggcg tcgtgattgc cgagaatcgc gtaggtggcg
     4561 gtttttatat gtcggcgcag gccgaccatc cggtgcgccg gcgtgtcgta atcctcgaac
     4621 gtaccttcgc ggaaatcgcc ggtgatcacg cacagatcgt attcgagcgg ctgaatcagc
     4681 cggatcagcg tatcgatcag ttccggattg ccgtcgatat gcgtgtcgct gatatgcagc
     4741 aggcggaacc cgtcgaaggc ttccggcagg tcggggcagg gcagttcctg gaaattcacc
     4801 cgcacggtca gggcgttgcg gtggccgcgc cagtaaagtc cggtgacctt gaacaggttg
     4861 cggatgatgc ggtggagcgt gcggatatgt tcaatatgga accagacctt gccgctgccg
     4921 aagagccgcg ccacgtggtg ggtctgaatt ttcaaccgct gcgccagcag tgtccggccc
     4981 agtcgttgct cgagctgttg atagtcgaca tcagccattg agatatcctt tcgccgcgac
     5041 tatattcgct catcaaccgg ctttaaagat gaataccgcc cgaaaaaaag aggagtcgcg
     5101 ccgcctcgat ggacagatcg agcggactgt tttccgcaac gaggaaaacg gctatgccgt
     5161 cctgcgcgtc aaagtgcgcg ggcacaagga ccttgtcacc gtggtcggca cggtctcgag
     5221 cgccaacccc ggcgagtggc tggccgccga cggcgagtgg attaccgacg cccattacgg
     5281 ccggcagttc aaagcggaaa ccatgcggat ttcccagccc gacacgctcg acggcatcaa
     5341 aaaatttctc ggctccggaa tgatccgcgg catcggcaaa gagtacgccg aacggctggt
     5401 gcaggcgttc ggacgcgacg tgtttgacgt catcgaaaac tcgtcggcca agctgctcaa
     5461 agtcgaaggg atcgggccgg gccggcgcgc cagcatccgc gcggcgtggg cggagcagaa
     5521 gggcatccgc gagatcatga gttttctctt cagccacggc gtcagcacgg cgcgcgcttt
     5581 ccggatttat aaagcctacg gcgagcggtc catcgaactg attcagcgcg acccgtactg
     5641 tctggcccgc gatattcacg gcatcgggtt tctgattgcc gacggcatcg cgcagaagct
     5701 cggcatcgcc aaagactccg acctgcgcgc ccgcgccgga atcgaatatg ttctgcagga
     5761 gctgacaacc aacgggcact gcgccgcgcc gcgcggcgac ctgcaaagca aaaccgtcga
     5821 gctgctcgac attccgcccg accggatttc cggcgcgctc gactggctga ttgccgccaa
     5881 acggctgatt ctggacgaag accggcaggg ccgcgcgctg gtctatctgc ccaagcttta
     5941 tttcgccgaa tgggccttcg ccaaaaaact gacggaactc aactacggca aacatccctg
     6001 cccgcaaatc gactttgaaa aagcgctcga gtgggtgcag aagaaaacca aaatccagct
     6061 gtcggaaaac cagtgcgaag cgctgcgcct cgcggtgcag gccaaagtga tggtgatcac
     6121 cggcggcccc ggcgtcggta aaaccacgct ggtgaactcg atcattcagg tgctggtggc
     6181 gaaaaagctg gcggtgtcgc tctgcgctcc gacgggccgc gccgccaagc ggctgagcga
     6241 aacgacgggc ttggaagcca aaaccattca ccggttgctg caattcaatc ccggcaccgg
     6301 cggcttccgg cataccgaag acaatccgct cgaaggcgat gtctttatca tcgacgaagc
     6361 cagcatgatc gatcaggtgc tggcctatca gctgttgcag gccgtgccgc agcgcgcggc
     6421 ggtgattttt gtcggcgatt ccgaccagct tccgtcggtc gggccgggcc gggtgctgtg
     6481 cgacatcatc gcgtccggcg ccgtgccggt tgtcgcgctg accgaaattt tccggcaggc
     6541 tgcggagagc cggattgtca ccggcgcgca ccgcatcaat cacggacagc tgccggaatt
     6601 tccggaagag ggcgataaaa cgtccgacct ttattttgtc gaagccgaag aggcgcagaa
     6661 ggtgatcggc gtcatcagca agatggtcag cgaatccatt ccgcagcggc tgggttttaa
     6721 tccggtggaa gatattcaga ttctgacgcc gatgcagcgc ggcgagctgg gcgcgcgcaa
     6781 tctcaatcag gtgatgcagg g
//
LOCUS       DAOPZO010000098         6789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_13675_length_6789_cov_8.197654, whole genome shotgun sequence.
ACCESSION   DAOPZO010000098 DAOPZO010000000
VERSION     DAOPZO010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13675_length_6789_cov_8.197654"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PKI68_06925"
     CDS             <1..530
                     /locus_tag="PKI68_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53462.1"
                     /translation="EVYSNTGGQMSKSTPIGAVAQFAAAGKRVGKKNLGFMCMSYGYV
                     YVASISMGANRMQTQKALMEAAAYDGPSIVIAYAPCIAHGIDMMKTQVEQKRAVDCGY
                     WPLYRYNPAAEEGKRFIWECKPPTASFQDFIRSERRYTALLKAAPQEAEAIFKAAEED
                     AKRRMAFMENLGKMM"
     gene            555..716
                     /locus_tag="PKI68_06930"
     CDS             555..716
                     /locus_tag="PKI68_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNX53463.1"
                     /translation="MKKFRCIPCGYIYDPAVGDPNNGIAAGTAFADLPADWVCPECGV
                     GKDYFEPAE"
     gene            752..1714
                     /locus_tag="PKI68_06935"
     CDS             752..1714
                     /locus_tag="PKI68_06935"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNX53464.1"
                     /translation="MNLKTTYMGLNLKNPIIVASSPVSQNIDDLRRAADAGAAAAVMF
                     SLFEEQIEKTDHPYFPSKEDYAISPDAYFEHLHQAVERTAIPIIGSLNGVTTKGWETY
                     ARKIEQTGAAGLELNIYHVAANLEETGADIEKRHLDILKAVRSAVKIPVAMKLSPYFS
                     SLGNMAKQFDEAGADALVLFNRFYQPDFNLDSMEVEATLDLSTAAEMRLPMRWIAMLH
                     GKLTASLGAAIGVRTGAEVVKYLLAGADAVQIASVLLKNGPEYTGTLISELKEWMQIK
                     EYDSVEQLKGAMSQRAVPDSDEFERANYIKVIETYKRDRANRTI"
     gene            1819..3438
                     /locus_tag="PKI68_06940"
     CDS             1819..3438
                     /locus_tag="PKI68_06940"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNX53465.1"
                     /translation="MGKQMAKYLIVTGGVVSSLGKGITAASIGRLLINRGLSIRMLKL
                     DPYLNVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERYTGQPTSQKSNITAGRIYWDV
                     LHKERRGDYLGATVQMIPHISNEIKFKIRQLESENPDVIVIELGGTVGDIEGLIFLEA
                     LRQLGADVGRDNSMYIHLTYVPYIRAAGEVKTKPTQQSVAKLREIGILPHIIVCRTEV
                     DLTDEHLDKLAMFCNVPKECVIVEKDVETSIYEIPLVLAEQGVDELILNHFRIAKPTR
                     SLKNWQKLISVIKNPKGKVRIGVVGKYSELQDAYKSIYEALYHGGYAAEYEVEIVKLH
                     AEEIEKNPDILKTVAGILIPGGFGSRGMEGKIRAAQFARENNIPFLGICLGLQCAVIE
                     FARNVCGLTGANSTEFDEERGVKTEHPVVCLLEEQRAVTQKGGTMRLGACPCVLEKGT
                     KALEAYGEKKISERHRHRYEVNNHYRDQLEKNGLIISGTNPDIGVVEMIELPDHPWYV
                     GTQAHPELKSQPVLPHPLFRDFVAAAIKNTL"
     gene            3435..4871
                     /locus_tag="PKI68_06945"
     CDS             3435..4871
                     /locus_tag="PKI68_06945"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX53466.1"
                     /translation="MKRFFSAVRSLTAAPDTSDPALLGQKTRKLSLWEGSLWSIMWGF
                     GETYIAPFALLLNAGNLAMAFVGTGPVLIAAVAQLAGSSMLDRIGRRMPVILTGSLIQ
                     ALAYLPLFFLPVLLPTAGKIPALLITVTVCFFAFGFSVPPWMSLMGDVVDQADRGRYF
                     SNRTRITMRAMLISMLIAGWIMNRWKLVGHPLVGFGFLFATASLARLVSVAFLRRHYD
                     APMQKEKDDSYFSFPDFIRATPRSNFAKFTLTVALMNGTAQISGPFFAVYMLRDLHWS
                     YLQFTVNVAAFLLSQTLFVRWWGALGDRHGNRSVLVATSCIMPFLPLIWISTDHYGIL
                     LFGQIISGACWSGFNLAASNFVYDAVTTPKRARAFSYYNLLNGLFSVAGGVLIGAPLA
                     SHLPSELRLGSWHIPFHSSLALVFIISSAARALAAWMMMPRFREVREAEPISTVRILW
                     RLGVGQPIFGQAGEFIPRIRKLYSPPGK"
     gene            complement(5203..5616)
                     /locus_tag="PKI68_06950"
     CDS             complement(5203..5616)
                     /locus_tag="PKI68_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53467.1"
                     /translation="MEQQWISVDLQPFRSFDQYAAVVRLGMFRGQRFGFADDLHQLDY
                     LEFAGMGFFSNVQQLAGKLIQPFAGGTEMFKSRVAFFRDVSAGAIADGQYIPDVVRKG
                     CGKQSGRGASFRRKQGFAMLLKFFLAPDNLPEKTE"
     gene            5620..5940
                     /locus_tag="PKI68_06955"
     CDS             5620..5940
                     /locus_tag="PKI68_06955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX53468.1"
                     /translation="MLLNLVLNAADAGCNRGTVLIRAVPVPNNVIIEVHDNGPGIPPS
                     DREKIFRSFYTTKPDGTGLGLLAVQACAKIHQGYIQVLSSGLGGACFRITLPINPPHE
                     KQPA"
     gene            complement(6049..>6789)
                     /locus_tag="PKI68_06960"
     CDS             complement(6049..>6789)
                     /locus_tag="PKI68_06960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX53469.1"
                     /translation="QSIYERSDVSVREKEGLPSRCGVLAGAEPPERVEINENGMRLLV
                     DIKGGHKTGYYLDQRDSRAAVRAWADGRDVLNCFSYTGGFGIAALLGGAKHVLQMDSS
                     EPALEIARENAALNGLDPAKSEYMCGDVFKELRRFRDSRRDFDLIILDPPKFVESRGQ
                     LEGGARGYKDINLLAFKLLRPGGLLFTFSCSGLMEMPLFQKIVSDAALDAGRNAQILA
                     VLNQAPDHPVALNFPEGAYLKGLLCRAE"
BASE COUNT         1560 a         2125 c         1736 g         1368 t
ORIGIN      
        1 ccgaagtgta ctccaacacc ggcggccaga tgtccaagtc caccccgatc ggcgcagtcg
       61 ctcagtttgc cgctgccggc aaacgggtcg gcaagaagaa cctcgggttc atgtgcatga
      121 gctacggcta tgtgtacgtc gcctcgattt cgatgggtgc caaccgcatg cagacgcaga
      181 aagcgctgat ggaagcggcc gcctatgacg ggccgtcgat cgtcatcgcc tacgctccgt
      241 gtatcgcgca cggtatcgac atgatgaaga cgcaggtcga acagaaacgg gcggttgatt
      301 gcggttactg gccgctctac cgctacaatc cggccgctga agaaggcaaa cgcttcatct
      361 gggaatgcaa gccgccgacc gccagcttcc aggacttcat ccgcagcgag cgccgctata
      421 ccgcgttgct caaggccgcc ccgcaggaag cggaagcgat cttcaaggcc gctgaagaag
      481 atgccaaacg ccgtatggca tttatggaaa atctcggtaa aatgatgtaa ttcggtaaac
      541 aaaggagata tccgatgaaa aagttcagat gcataccttg cggttacatt tacgacccgg
      601 cggttggcga tccgaataac ggcatcgccg ccggcacggc atttgccgac ctgcccgctg
      661 actgggtctg ccccgaatgc ggcgtaggca aagactactt cgaaccggct gaataacagc
      721 cggttcgaat aacggcaggg agcgactgat cgtgaattta aaaaccacat acatggggtt
      781 gaatcttaaa aacccgatca ttgtcgcctc ctcgccggta tcccagaaca ttgacgacct
      841 ccgcagagcg gccgacgccg gcgctgcggc ggccgttatg ttttctcttt tcgaagaaca
      901 aattgagaaa acagaccacc cctatttccc gtccaaagag gactatgcca tcagtcctga
      961 tgcttatttt gagcaccttc accaggcggt tgaacgcacc gcgattccaa tcattggaag
     1021 cctcaacgga gtcaccacaa agggatggga aacgtatgcc cgcaagattg agcagaccgg
     1081 cgcggcggga ctggaactga acatctatca cgtcgccgcc aaccttgaag aaaccggtgc
     1141 cgacattgaa aaacggcacc tcgacatcct gaaagccgtc agaagcgccg taaaaattcc
     1201 ggtcgccatg aaactcagcc cctatttcag ctcgctggga aacatggcca aacagtttga
     1261 tgaagccggc gccgacgctc tcgtcctttt caaccgtttt taccagcccg acttcaatct
     1321 cgactccatg gaagtcgaag ccacccttga cctcagcacg gcggccgaaa tgcgtctgcc
     1381 gatgcgctgg atcgccatgc tgcacggaaa gctgacggct tcattgggcg cagccatcgg
     1441 ggtgcgaacc ggcgcggaag ttgtcaaata tctgctggcc ggagccgatg ccgttcaaat
     1501 cgcatccgtt cttctcaaaa acggcccgga atacaccgga acactgattt cagagctcaa
     1561 ggaatggatg caaatcaagg aatacgattc cgtagaacag ctgaaaggcg ccatgagtca
     1621 gcgggcggtt cctgattccg acgagtttga acgggccaat tacatcaaag taattgaaac
     1681 gtataagcgc gaccgggcta accggacgat ttagcaatgc cggcggaaat gtttccaaac
     1741 attggaaact attctttgga caacctcgcg ccccatcctt aaagtgcctt cccgtagcaa
     1801 gcaatctaaa cagtgactat gggaaagcaa atggccaaat atcttatcgt aaccggcggc
     1861 gtcgtatcct cactcggcaa aggcattacc gccgcatcta tcggacgtct cctgatcaac
     1921 cgcggactca gcatccgcat gctgaaactc gacccctatc tcaacgtaga ccccggcacc
     1981 atgagccctt atcagcatgg tgaagtgtac gtcaccgacg acggcgcaga aaccgacctc
     2041 gacctcggcc actacgagcg ctacaccggc cagcccacct cgcagaaaag caatatcacg
     2101 gcaggccgca tctactggga cgttctgcat aaagaacggc gcggcgacta cctcggcgcc
     2161 accgttcaga tgattccgca catctccaac gaaattaaat ttaaaatccg ccagctcgaa
     2221 tccgaaaacc ccgatgtaat cgtcattgaa ctcggcggca ccgtgggcga catcgaaggc
     2281 ctgatttttc tcgaagcgct gcgccagctc ggcgcagacg tcggccgcga caattccatg
     2341 tacattcacc tcacgtacgt cccgtacatc cgggccgccg gcgaagtaaa aaccaaaccc
     2401 acccagcaga gcgtcgccaa actgcgcgaa atcggcatcc tgccgcacat tatcgtctgc
     2461 cgcaccgaag tggatctgac cgatgagcac ctcgacaaac tggctatgtt ctgtaacgtg
     2521 ccgaaagaat gcgtcatcgt cgaaaaagat gtcgaaacat ccatctacga aattccgctc
     2581 gtgctggctg aacaaggtgt ggacgaactg atcctcaacc acttccgcat tgcgaaaccc
     2641 accagatcgc ttaaaaactg gcagaagctg atctctgtca tcaaaaatcc gaagggcaaa
     2701 gtccgcatcg gcgtcgtcgg caaatattca gaactgcagg acgcctacaa atcaatctac
     2761 gaagcgctct atcacggcgg ctatgcggcg gaatacgaag tggaaattgt aaaactccac
     2821 gccgaagaga ttgagaaaaa tcccgacatt ctgaaaaccg tcgccggcat tctcattccc
     2881 ggcggattcg gttcgcgagg aatggaaggt aaaatccgtg cggcacagtt tgcgcgcgaa
     2941 aataacatcc ccttcctcgg catctgcctc ggccttcaat gcgcggtaat cgaattcgcc
     3001 cgcaatgtct gcggactgac cggcgcgaac agcaccgagt tcgatgaaga acgcggcgta
     3061 aagaccgaac atccggtcgt ctgcctgctt gaagaacagc gggccgtcac gcagaaaggc
     3121 ggcaccatgc gcctcggcgc ctgcccgtgt gtgcttgaaa aaggcaccaa agcacttgag
     3181 gcctacggcg aaaagaaaat ctcagaacgc caccgccacc gctacgaggt caacaatcac
     3241 taccgcgacc agctggagaa aaacggactc atcatttccg gcaccaatcc ggacatcggc
     3301 gtcgttgaaa tgattgaact gccggaccat ccgtggtacg tcggcacaca ggctcatcct
     3361 gaactgaaga gccagccggt actcccgcat ccgctcttcc gcgacttcgt agccgccgcg
     3421 atcaagaaca ccctatgaag agattcttct cagcggtgcg cagcctcacc gcagcgccgg
     3481 acacttcgga tccggcgctt cttggacaaa aaacccgcaa gctctccctc tgggaaggtt
     3541 cgctgtggtc catcatgtgg ggcttcggcg aaacctacat cgctccgttc gccctgcttt
     3601 tgaacgccgg caatctggct atggccttcg tcggcaccgg cccggtattg attgccgccg
     3661 tcgcccagct ggccggatcg tccatgctgg accggatcgg acggcgcatg ccggttatcc
     3721 tgaccggatc cctgatccag gcgctggcct atctgccgct cttttttctg ccggtcctgc
     3781 tgccgaccgc cggcaaaatt ccggcgctgc tcatcaccgt caccgtctgc tttttcgcct
     3841 tcggcttttc tgtcccgccg tggatgagcc tcatgggtga tgtagtcgat caggccgacc
     3901 ggggccgcta cttctccaac cgcacccgca tcaccatgcg cgcgatgctg atttcgatgc
     3961 tgattgcggg ctggatcatg aaccgctgga aactcgtcgg ccatccgctc gtcggcttcg
     4021 gctttctgtt cgccaccgcc agcctggccc ggctggtgag cgttgccttc ctgcgccgcc
     4081 actacgatgc gccgatgcag aaagaaaaag acgactccta tttttcattt ccggatttca
     4141 tccgcgccac cccgcgctcc aacttcgcca aattcaccct caccgttgcg ctcatgaacg
     4201 gcacagcaca gatatccggc cccttcttcg ccgtctacat gctgcgcgac ctgcactggt
     4261 cctacctcca gttcaccgtc aacgtcgccg ccttcctgtt gtcgcaaacc ctcttcgtgc
     4321 gctggtgggg cgcgcttggc gaccggcacg gaaaccgttc cgtgctggtc gccaccagct
     4381 gcatcatgcc gtttctgccg ctcatctgga tttcaaccga tcactacggg atcttactct
     4441 ttgggcagat catctccggc gcctgctggt ccgggtttaa cctggcggcc tcaaactttg
     4501 tctacgatgc cgtcacaacg cccaaacgcg cacgcgcctt cagctactac aatctgctca
     4561 acggactgtt ctcggtggcg ggcggcgtac tcatcggcgc tccgctggcc agccacctgc
     4621 cgtcggagct gcgcctcggc agctggcata ttccattcca ctcctcgctg gcgctggtct
     4681 tcatcatctc cagcgcagca cgggcgctgg cggcatggat gatgatgccg cgcttccgcg
     4741 aagtccgcga agcggaaccg atctccaccg tccgcattct gtggaggctg ggcgtcgggc
     4801 agccgatttt cggacaggcc ggtgaattca ttccccgcat acgtaagctc tattctccgc
     4861 cgggaaaata atcattttca taaaacccgg ccaatcctcc ggtctcttta accttgcaca
     4921 gcaacggcct ttgtattttc attccggtga aagcggaaat gcaaattcag gaggaggctt
     4981 atgccggaat cgcctgcagc accgaaactt accccgcccg ttcatccatt gcgggcatcg
     5041 ggactcctga ccggaaccta tctcatcgtc tgtatcctct acatcgtcat ctcaagcaca
     5101 ttggcagctc agttttcagt atcactcact gaaatgaagc ggattgagct cttcaaagga
     5161 atcctgttta tcgtgctgac gggtttactg ctgttctttt tttcactccg tcttttccgg
     5221 caggttgtcc ggcgcgagaa agaacttcag cagcatcgcg aagccctgct tgcggcggaa
     5281 cgacgcgcca cggccggact gtttgccgca tcccttgcgc acgacatccg gaatgtattg
     5341 accgtctgca attgcgccag cgctgacatc tctgaaaaaa gcaacccggc ttttgaacat
     5401 ctcagtgccg cctgcaaacg gctgaatcag ctttccggcc aactgctgga cattggagaa
     5461 gaaacccata cctgcgaatt caagatagtc gagctgatgc aaatcatcag cgaaaccaaa
     5521 gcgctggcct cggaacatac caaggcgcac cactgccgca tactgatcga aggaccggaa
     5581 cggctggaga tccacgctaa tccactgctg ctccatcaaa tgctcttgaa ccttgtgctg
     5641 aacgcggcgg acgccggctg caaccgggga accgtactga tccgcgccgt gccggttccg
     5701 aacaacgtaa taatcgaagt acacgataac gggccgggca ttccgccttc cgaccgcgaa
     5761 aaaatattcc gctcattcta caccacgaaa cccgacggaa ccgggctggg attactggcc
     5821 gttcaagcct gcgccaaaat acaccaaggc tatatccagg tgctttcttc ggggctggga
     5881 ggcgcctgct tccgcatcac gttaccgatc aacccgccgc acgaaaaaca accggcctga
     5941 ctgactttta tgccgtgtct ttattaacag gcgctttggg tacggctgtt tgcttgcaaa
     6001 cggccctgcc gttataggcc gtttgaccag caaacggctg tacaaatttt actccgcgcg
     6061 gcagagcagc cctttgagat aagcgccttc cggaaaattc agcgccaccg gatgatccgg
     6121 agcctggttc agcaccgcca gaatctgggc gttgcgcccc gcatccaacg ccgcgtccga
     6181 aacaattttc tgaaacagcg gcatctccat cagcccggaa caggaaaacg taaagagcag
     6241 tccgccgggc cgcagcagct tgaacgccag cagattgatg tctttatagc cgcgcgcgcc
     6301 gccttcaagc tgaccgcgtg attccacaaa cttcggcgga tcgagaataa tcagatcaaa
     6361 gtcgcgccgc gaatcgcgga agcggcgcaa ctccttaaac acatcgccgc acatatattc
     6421 cgacttcgcc ggatcaagcc cgttcagcgc cgcattctcc cgcgcaatct ccagcgccgg
     6481 ttctgaactg tccatttgca gaacatgctt tgcaccgccg agaagcgcgg cgatgccgaa
     6541 gccgccggtg taggaaaaac agttcagcac atcgcggcca tcggcccagg cgcgcaccgc
     6601 ggcacggctg tcgcgctgat cgaggtaata accggtcttg tgcccgccct taatgtccac
     6661 cagcagacgc ataccgtttt cgttgatttc aacgcgttcc ggcggctccg ctccggccag
     6721 tacgccgcaa cgcgacggca gaccttcctt ttcgcgtacc gaaacatcgg agcgctcata
     6781 aatggactg
//
LOCUS       DAOPZO010000099         6727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_13851_length_6727_cov_7.951739, whole genome shotgun sequence.
ACCESSION   DAOPZO010000099 DAOPZO010000000
VERSION     DAOPZO010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13851_length_6727_cov_7.951739"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="PKI68_06965"
     CDS             <1..831
                     /locus_tag="PKI68_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53470.1"
                     /translation="PDLLNAIQALYQLSYTPTKGRKKRGLYVMFFGVSTFIRPDSFVD
                     VKKFLKFIIGLFFLPLCWAFSKVVFSLLQSLPAAASGWTIWALPLGFMISIVGFFILP
                     RPFRTYVLAHELTHAVWGLLMGAKVGKMKVGKSGGHVMLSKSNFIISLAPYFFPFYTV
                     LVIGLWYGLGCFFDVSRYEVWWLAAVGLTWGFHVTFTVYMLSQHQPDIQENGRLFSYV
                     IIYLASLFFVALWVIVIGSPTFRGAWQLLLPETAADYESVRVVFLAGWSHLKDIQKSP
                     "
     gene            886..1500
                     /locus_tag="PKI68_06970"
     CDS             886..1500
                     /locus_tag="PKI68_06970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX53471.1"
                     /translation="MKTARENNPMDQEDDAELLAAYRAGDGESLGKLVEKYKRPLFGF
                     ILRFSEGREEADEIFQEVWVRAIKNMNRYHHKNLLSWLFRIAHNLMIDRMRRSKPMLS
                     LDTPMNTEGSPLGEQLAAVCLGPDGEAGGRELGLRIEAAAAQLPAEQREVFWLRMQAD
                     LSFKEIAKIQKCSINTALARMQYAVSKLRKELAGEYRELQKAGI"
     gene            1497..1952
                     /locus_tag="PKI68_06975"
     CDS             1497..1952
                     /locus_tag="PKI68_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53472.1"
                     /translation="MKTKKLEKWLLLEQSGELSPRQLRRLNRELSDSEEARVLRSELR
                     RLKGVLLTPDIEPSPWTVSQIAARLRVENRPDMALIRILKPVLALAACLALMLGIGNF
                     HGERASSASPGVVASAGVDVWDDPIGEDLNNLENLIVAISGDSNDVMEM"
     gene            1968..2588
                     /locus_tag="PKI68_06980"
     CDS             1968..2588
                     /locus_tag="PKI68_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53473.1"
                     /translation="MKKWMIYVLIGACAAGMATAEETVPWYKKLFTKNADEETQVIPP
                     APAVTAPASAPEVKQGQRPALTPEQKERMKAHAQLQQREGAGPRPMMTGEQAEKMKAQ
                     REELNKLGAAARNETDPARKEALIAGLRAKLTAIADQRQAENKKRLEKAEQDLPKLKA
                     QIEELEKNKTDRIEKQLQAILSGEPFKAGESGDRKGKKAGKAPVAK"
     gene            complement(2649..3479)
                     /locus_tag="PKI68_06985"
     CDS             complement(2649..3479)
                     /locus_tag="PKI68_06985"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNX53474.1"
                     /translation="MSRATFQTKPNEDGLPLVDVLSDRLRCSKKQARVLLDARQVFVN
                     GKRVWMAKHTLKLKDVIETVRPEPKSHKIEILKRAGDLIVVNKPSGMVTNGSARSLEV
                     RLQHELNNPQLCAVHRLDRDTSGCVLFAANAAAKEKMIPLFKEERIVKIYRAIAVGRV
                     SDQLKTITRDIDGESATTMVSVLDRNRDASYLELRIHTGRTHQIRKHLSGVRHPVLGD
                     KEYAGEQRGNPVFRSVPRQMLHACRLILPDPQGGAAALRVTAPVPDDFQAVLDTLKLK
                     "
     gene            complement(3479..4669)
                     /locus_tag="PKI68_06990"
     CDS             complement(3479..4669)
                     /locus_tag="PKI68_06990"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNX53475.1"
                     /translation="MKTEEIIQLHKKYVMPTYGPPSLALVKGSGSNVWDADGKEYLDF
                     LAGIAVLNVGHCHPAVVKAVQEQAAKLVHVSNLYCNENQPRLAQALSERSMGGKCFFC
                     NSGAEANEGLIKLARLWGSTKGKYEVITMKNSFHGRTLATLTATGQEKVQKGFYPLPE
                     GFKYAEFNNLESCRAAVTDKTAAILVEAVQGEGGILPADPAFIKGLRKLCDEKDILLF
                     FDEVQAGMGRTGKWFGFQNYDVQPDAFSLAKGLGNGFPIGAVVASPKMSDIFQPGNHA
                     TTFGGTPLACAAALAVIETIEKEQLLENARKTGALLLEKLQAIAAKYDWIEGARGCGL
                     MVGLVLKDNAAPLQKRLQEKGLLTLATAVRVLRMLPPLNITVEEIEKAAVLIAEACAE
                     MDKA"
     gene            complement(4831..6696)
                     /locus_tag="PKI68_06995"
     CDS             complement(4831..6696)
                     /locus_tag="PKI68_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53476.1"
                     /translation="MNTLLKVMAIAITLSLGKVYAESAGGSDWKYNETNPSNKICFAM
                     YTVEKRVLKLTAQLYPLQAQESRDIFLEIKRDNVWQRVAQSKVRENEYAVHDAKAWNT
                     LFRVENWDHSRDWEYRVVALDGQATYSGIIRKDPADKEEIVVATFTGNSNKDRRMKPD
                     IIANIKAQNPDLLFFSGDQSYDEEDHLPAWLLFGRQFGEIIKDRPTICIPDDHDVGQG
                     NLWGAEGKKSTGAQGCADGGYILPVDYVNEVQFAQTANLPDPYDPTPIQRNISVYYTS
                     LNIGGVDFAIIEDRKFKTGPLGLVKAPHGDRVDLINDPNYDRASVDVPGAELLGSRQI
                     KFLSDWGTHWEGVQMKCVLSQTIFVNAAHKTGKWRVVADLDSNGWPQTGRNQALKEIR
                     KSFSFMIAGDQHLGTVIHHGTDEWRDAGFSFCVPSIVNFWPRSWLPLEKGLNPVSDAL
                     EHTGDYLDGFGNKLTMYAYSNPRGATGDIGTRDDPFAGGAAGYGIVRFNKKARTITAE
                     CWPRNVDVTAPDAKQYPGWPVTVDQQDNYGRKPAAYLPELNIEGETNPVVQVIHEATN
                     EILYTIRIKGNLFRPKVFADGKYTVRIGEGKNVRTLTGVESIGLNEKRTITVKLK"
BASE COUNT         1442 a         1772 c         1883 g         1630 t
ORIGIN      
        1 cctgacctct tgaatgccat tcaagcgctc taccaactga gctatacccc cacaaaaggt
       61 cgaaagaagc gtggtttata cgtgatgttt tttggagtgt caaccttcat tcggcctgat
      121 agtttcgttg atgtgaaaaa gtttctgaaa ttcatcatcg ggctgttttt ccttccgctg
      181 tgctgggctt tttccaaggt cgttttctcg cttctgcagt ctctcccggc ggcagcgtcc
      241 ggctggacga tatgggcttt gccgctggga tttatgatct ctatcgttgg tttttttatc
      301 ctgccccggc ctttccgtac atatgtgctg gctcatgagc tcacccatgc tgtatggggc
      361 cttctgatgg gtgcgaaggt cgggaagatg aaggtgggaa aaagcggcgg acatgtaatg
      421 ctgtcgaaaa gcaatttcat catctctctg gcgccgtatt ttttcccgtt ctatacggtt
      481 ctggtgatcg gtctctggta tgggcttggc tgtttttttg atgtaagccg ctatgaggtt
      541 tggtggctgg cggcggtcgg gctgacgtgg ggttttcacg tcacctttac ggtttatatg
      601 ctcagccagc accagccgga cattcaggaa aacggccggc tgttttccta tgtgatcatc
      661 taccttgcca gcctgttttt cgtcgcgctt tgggtgattg taatcggttc gccgacgttc
      721 cgcggtgcgt ggcagctgtt gctgccggaa acagccgcag attatgagtc agtaagggtg
      781 gtatttcttg ccggatggag ccatcttaag gatattcaga aaagtccatg atctccggaa
      841 ataagatacg ggtacaataa agcaaactcc aactcgttat atgtcatgaa aaccgccaga
      901 gagaataatc cgatggatca ggaagatgat gcagagctct tggccgctta tcgtgcaggt
      961 gacggtgaat ctctcggtaa actggtcgaa aaatataaac gtccgctctt cgggtttatt
     1021 ctgcgattct cggaaggccg ggaagaggct gatgaaattt ttcaggaagt atgggttaga
     1081 gcgattaaaa acatgaaccg ctatcaccat aagaatctgc ttagctggct gtttcgcata
     1141 gcccacaatc tgatgattga ccggatgcgg cgctcaaaac ccatgctttc gctggatacg
     1201 ccgatgaata cggaagggtc tccgcttggc gaacagcttg ccgctgtttg tctcggcccg
     1261 gacggtgaag ccggcggacg cgaactgggg cttcgcatcg aagccgccgc ggcgcagctt
     1321 ccggccgagc agcgtgaggt tttctggctg cggatgcagg ccgatcttag ttttaaggaa
     1381 attgccaaaa tacagaagtg ttctataaat acggcgctcg cccggatgca atacgctgtt
     1441 tccaagctcc ggaaagaact ggccggtgaa taccgcgaat tgcagaaagc tggaatatga
     1501 aaacaaagaa actcgaaaaa tggctgttac tcgaacagtc cggtgaactt tcgccccggc
     1561 agctccgccg gcttaatcgc gaactgtcgg attcggaaga ggcccgcgtc ctgcgcagcg
     1621 aactccgccg gttgaaaggt gtacttctga caccggatat tgagccttcg ccgtggactg
     1681 tttctcagat cgcggcccgg ttgcgggtcg aaaaccgtcc ggatatggct ctgatccgta
     1741 ttttgaagcc ggttcttgcg ctggcggcct gtctggctct catgctgggc atcggaaatt
     1801 tccatggcga gcgggcctct tcagcttccc ccggtgttgt tgcatcggcg ggagtggacg
     1861 tgtgggacga tccgatcgga gaggatttga acaatctgga aaacctgatt gtggcgatat
     1921 cgggtgattc gaatgatgtc atggaaatgt aaccggagga gagaactatg aaaaagtgga
     1981 tgatatatgt cttaatcggc gcatgtgccg ccggtatggc gaccgcggaa gaaacagtac
     2041 cctggtataa aaagctgttt acaaaaaacg cagacgaaga gactcaggta attcctccgg
     2101 ctccggcggt aacggctccc gcctcggctc cggaagtaaa acaaggccag cgtccggctc
     2161 tcacacccga acagaaagaa cggatgaaag cccatgctca actgcagcag cgtgaaggcg
     2221 caggtccgcg tcctatgatg actggcgaac aggctgaaaa gatgaaagct cagcgtgaag
     2281 agctgaataa gctcggcgca gccgcccgga atgaaaccga tccggcccgc aaagaagctt
     2341 taattgccgg cctgcgcgcc aagctcacag caattgccga ccagcgtcag gccgagaata
     2401 agaagaggct cgaaaaggcg gagcaggatc ttccgaagct caaggctcaa atcgaagaat
     2461 tggaaaaaaa caaaacggac cggatcgaga aacagcttca ggcgattttg tccggcgagc
     2521 cgtttaaagc aggagagtcc ggcgaccgga aaggtaaaaa agccggcaag gcgcctgttg
     2581 caaagtaatg ttgtcgatat agcagcaaac ccccgaaacc ccgccgatgg cggggtttca
     2641 tttttccatt acttcagttt cagcgtatcg agaaccgcct gaaaatcgtc gggtaccggc
     2701 gcggtaacgc gcagcgcagc ggcgccgccc tgcggatccg gcagaatcag cctgcaggcg
     2761 tgaagcatct gtcgcggtac cgaacggaag accgggttgc cgcgctgttc gccggcatat
     2821 tccttatcgc ccagaaccgg gtgacgcacg ccggaaagat gcttgcggat ctggtgcgta
     2881 cggcctgtgt gaatacgcag ctccagatag ctggcgtcgc ggttgcggtc gaggacgctg
     2941 accatggttg tggcggattc gccgtcaatg tcgcgcgtga tggttttgag ctgatctgaa
     3001 acgcgtccga cggcgatggc acggtagatt ttgacgatcc gctcttcttt gaaaagcgga
     3061 atcatctttt ccttggcggc ggcgttggcg gcgaacagga cgcagccgga tgtgtcgcgg
     3121 tcaaggcggt gtaccgcaca gagttgagga ttattcagtt cgtgctgcaa gcggacttcc
     3181 agactgcgcg cgctgccgtt cgttaccatg ccggacggct tgttgacgac gatcagatcg
     3241 ccagcccgct tcagaatttc aattttatgc gatttcggtt cggggcggac cgtctcgata
     3301 acgtccttga gcttgagcgt gtgttttgcc atccagacac gtttgccgtt gacgaaaacc
     3361 tggcgtgcgt cgagcagtac gcgcgcctgt tttttggaac agcgcagccg gtcggaaaga
     3421 acgtccacca gcggcaggcc gtcttcgttc ggtttcgtct ggaaagttgc acggctcatc
     3481 aggctttatc catttctgcg caggcttcgg cgataagcac ggcggctttt tcgatttctt
     3541 caacggtgat atttaacggc ggaagcatcc gcaacacccg cacggcggtg gcgagagtga
     3601 gcagaccttt ttcctgcaac cgtttctgga gcggtgcggc gttatctttt agtacgagac
     3661 cgaccatcag gccgcaaccc cgtgcgccct cgatccagtc atacttggcg gcgattgcct
     3721 gaagtttttc cagcagcagc gcgcccgtct tccgggcgtt ttcgagcagc tgttcttttt
     3781 cgatcgtttc gatgacggcc agcgcggcgg cgcaggccag cggggttccg ccgaacgtgg
     3841 tcgcgtggtt gccgggctgg aaaatgtctg acattttcgg cgaggcgacg accgcgccga
     3901 tcgggaaccc gttgccgagt cctttggcca gcgagaacgc atccggttgt acatcatagt
     3961 tctggaatcc gaaccatttt cctgtccgtc ccatgcccgc ctgtacttcg tcgaaaaaca
     4021 gcagaatatc tttttcgtcg cagagtttcc gcagtccttt gatgaaagcc ggatcggcgg
     4081 gaagaatacc gccttcgccc tgaactgctt caacgaggat tgcggcggtc ttgtccgtca
     4141 cagcggcccg gcacgattcg aggttgttga actcggcgta cttgaaacct tcgggcaggg
     4201 gatagaaacc tttttgaacc ttttcctgtc cggtggccgt cagcgtggcc agcgtccgtc
     4261 catgaaagga gtttttcatg gtgatgactt cgtatttacc tttagtcgaa ccccacagcc
     4321 gtgcgagttt gatcagccct tcattggctt ccgcaccgga gttgcagaaa aagcattttc
     4381 cgcccatcga ccgctcggaa agggcctgag caagccgcgg ctgattttcg ttgcagtaga
     4441 gattggagac atgcaccagc ttcgccgcct gttcctgtac ggctttaacg acagccggat
     4501 ggcagtgtcc gacgttcaat acggcgatgc cggccaggaa atcgaggtat tcttttccgt
     4561 cggcatccca tacgttgctg ccggagcctt tgacgagcgc aaggctcggc ggcccgtaag
     4621 tcggcatgac atactttttg tgcaactgga taatttcttc tgttttcatg acttccattc
     4681 cctaaaagac gggggaatgt aacgcacgtc cgccggaaat gccagtggtt taaaaagaaa
     4741 gagcccatag gccgggaaac taaacgtgac ctgacgttgc ggatcaggcg cgacgggctg
     4801 tcgtcgcctg tatccggctt gttcgacagt ttacttcaat ttcacggtga ttgttctttt
     4861 ttcgttcagg ccgatggact caacacccgt tagcgttcgt acgttcttcc cctcgccaat
     4921 ccggacggta tatttcccgt ctgcgaagac tttgggccgg aacagattgc ctttgatgcg
     4981 aatcgtataa agaatttcat tggtcgcctc gtgaatcacc tgcacgacag gatttgtctc
     5041 gccctcaatg ttcagttcgg gcagataggc cgccggcttg cgtccatagt tatcttgctg
     5101 atcaaccgtc accggccagc cgggatactg cttggcatcc ggagccgtga catccacatt
     5161 gcgcggccag cattctgcgg taatggtacg ggctttcttg ttaaatcgga caatcccgta
     5221 cccggcggca ccgccggcaa agggatcgtc tcgggttcct atatctccgg ttgccccacg
     5281 ggggttcgag taggcataca tggtcaattt gttgccgaat ccgtcaagat aatcgcccgt
     5341 gtgttccagc gcatcggata cagggttaag gcctttctca aggggaagcc atgacctggg
     5401 ccagaagttg acaatggatg gcacgcagaa agagaacccg gcatcgcgcc actcgtctgt
     5461 accgtgatga atcactgtgc cgagatgttg gtctcccgca atcatgaagg aaaaggattt
     5521 ccggatttct ttgagcgcct ggttgcgacc cgtttgcggc cagccgttgg aatcaaggtc
     5581 ggcaacgacc cgccactttc cggttttgtg ggctgcgttc acaaagatcg tttgcgaaag
     5641 aacgcatttc atctgaacgc cttcccaatg tgtcccccag tcgcttagga acttgatctg
     5701 tctgctgccg agaagctctg cgccgggaac atcgacagat gcgcgatcat aattagggtc
     5761 attgatcaga tcgacacggt cgccgtgggg agctttgacc agtcccaggg ggcccgtctt
     5821 gaacttgcga tcctcgataa tcgcgaaatc cactccgcca atgttcagcg acgtatagta
     5881 tacgctgata ttgcgctgaa tcggtgtggg atcgtatggg tcgggtaggt tcgccgtctg
     5941 ggcgaactgc acttcattca cgtaatctac cggcaagatg tacccaccat ccgcacagcc
     6001 ttgagcgccc gtggatttct tgccttcggc gccccaaaga tttccctggc cgacatcgtg
     6061 gtcgtccgga atgcagatgg tgggtctgtc cttgatgatt tcgccgaatt gtctgccgaa
     6121 cagaagccat gccggcaaat gatcttcttc gtcataggac tggtcgccgg aaaaaaacag
     6181 cagatcggga ttctgcgcct tgatattggc tatgatatcc ggcttcatcc gccggtcctt
     6241 gttggagttg ccggtaaatg tcgcaacaac gatctcctcc ttatcggcag gatccttgcg
     6301 aataatcccg ctgtaggtag cctggccatc cagcgcgacg acccgatact cccaatccct
     6361 gctatgatcc cagttctcaa cacgaaacag cgtgttccag gccttggcat catgaacggc
     6421 gtactcattt tctcgaacct tgctttgtgc cacccgctgc cacacattat cccgcttgat
     6481 ctccagaaaa atgtcacgac tctcctgggc ttgaagcgga taaagctgcg ccgtcagttt
     6541 gaggacccgt ttctcaactg tatacatcgc gaagcaaatc ttatttgagg gattcgtctc
     6601 gttgtacttc caatccgacc cgccagcact ttcggcataa accttgccta gggatagagt
     6661 gatcgctata gccatcactt ttaacaatgt attcatgatg tttcctttcg aggttgttga
     6721 actcggc
//
LOCUS       DAOPZO010000100         6683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_13984_length_6683_cov_8.368588, whole genome shotgun sequence.
ACCESSION   DAOPZO010000100 DAOPZO010000000
VERSION     DAOPZO010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13984_length_6683_cov_8.368588"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="PKI68_07000"
     CDS             complement(<1..1039)
                     /locus_tag="PKI68_07000"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HNX53477.1"
                     /translation="MGNKIRRVFFAFGCVLLFREVAVGSPVTQDQAAQAANTFITLTY
                     PSGGLQTAMTTTAAGQSARAVQSVRPVVGSGGQTIGYVAELDSTGYVLLSSDDEAPPV
                     KCYTDSGTYDQLPPEFLNILNLELAEDLSVLSGMTQTKSGGFSGVSQTQSAGVSGYKE
                     QWLALLNPALPTKAPEAMSTSAAAGTVLLTTTWDQADPYNYYCPSAVGGPGGRSYAGC
                     TACAVSQILRYWSRPNAILSGHSYYDSKGSCTGNRSISDAGMTAYNWANMPSLITTGS
                     STLQKQAVGQLMYHAAVALDSDFESSGTAAYPTDVPSVLRTYFAYACSNYSQKASFTS
                     TGWYNQIASDVG"
     gene            complement(1353..1715)
                     /locus_tag="PKI68_07005"
     CDS             complement(1353..1715)
                     /locus_tag="PKI68_07005"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNX53478.1"
                     /translation="MDRKSGGRDFHLLEREIRARKNPSYDAVCFHAEQCAEKYLKGRL
                     TEAGLVFPKTHDLVLLLDLSLPVEPLWEICRNDLSFLSGFGVEFRYPGESADLAMAKE
                     ALKRCRIFRKMARETMHL"
     gene            complement(1716..2036)
                     /locus_tag="PKI68_07010"
     CDS             complement(1716..2036)
                     /locus_tag="PKI68_07010"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNX53479.1"
                     /translation="MVSANKIHACAREVAEKFHPEKIVLFGSYAYGTPTTDSDIDLLV
                     VMNHKSSAVAQAAEIRASLRSVFPIDVLVRSPRKIRERLKMGDPFFKTIVEKGEVLYE
                     TPDA"
     gene            2211..3278
                     /locus_tag="PKI68_07015"
     CDS             2211..3278
                     /locus_tag="PKI68_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53480.1"
                     /translation="MKKTLVLLFALAAGSIYGAMPVEKSITHLSTPVQLSVDEYHSLF
                     PKDYGVHGLRLNCYFADNRFMYGLDLGFWNVSEDAGGLQMAIYRNETHDFGGVQLSLW
                     NAETKDVGGIQVATVTTDAENLYGVQLTGLLGRAREVNGVQIGGLSAVSESETDKCWT
                     KGFQAGLYEARAENLAGVQIGGVFTETGWYANGVQIGLLFNESRYTRGVQLGGLTAKS
                     KETRGVQIGGLMAKSEIAADGVVQAAVILAETGEMKGLQLSVVSANVIGKADGVQVGG
                     VSTMAGALDGLEVAGIWNFVFENVNGAQVGLIYNHARYVRGLQLGLINHCERLDGVQI
                     GLINTVKEARFSTCPLLRVDF"
     gene            3363..4256
                     /locus_tag="PKI68_07020"
     CDS             3363..4256
                     /locus_tag="PKI68_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX53481.1"
                     /translation="MIKKGGTYVVMGLLNTDSIAYATGRMIEELGGKVIYTVQNERLK
                     KIFFDRGCTDISQEEKDAMDIRYCDVTIEEEVRNLFKEIGDLNGIVHSIGFANPKTCL
                     GDHIYTDAIEDVKQGFHISCASLATVSQFAQEHMHKGGSIVTLSFASQVAFPFYNWMG
                     VNKAALEALVRALAREYGREHVRVNAVSAGPLATKAAKSIPHFAHLARVWKKMSPLPW
                     DVINDKKEVASAVVFLLGEGSKKITGQTLYVDGGASVVGGELLPNERPIKAMPAKRKA
                     AAKERLERMRELQKQHQQQGL"
     assembly_gap    4272..4371
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4416..4490)
                     /locus_tag="PKI68_07025"
     tRNA            complement(4416..4490)
                     /locus_tag="PKI68_07025"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4455..4457),aa:Glu,seq:ttc)
     regulatory      4591..4696
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4723..4923
                     /gene="thiS"
                     /locus_tag="PKI68_07030"
     CDS             4723..4923
                     /gene="thiS"
                     /locus_tag="PKI68_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNX53482.1"
                     /translation="MYLTVNDERHEHRGDRTVPALLAELGAMPEHTALTVNGDLVFSR
                     DWKTFKFNDGDTVEVLTFVGGG"
     gene            4925..5707
                     /locus_tag="PKI68_07035"
     CDS             4925..5707
                     /locus_tag="PKI68_07035"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNX53483.1"
                     /translation="MNPETGTLKLGGHEFKSRLILGTGKFSDTATMLAAVEASGTELV
                     TVALRRFNRQKPGDDLCAPLARLQGIRLMPNTSGAMNAKEAVRAAQLGRELSGSPFVK
                     VEIHPNPHHLMPDAIETYLAAKELAADGFVVLPYIPADPVLAKKLEDVGCAAVMPLGA
                     AIGSGGGLSTAEMIRLIIRDSTIPVIVDAGLRAPSEAARAMEMGCDAVLVNSAIAAAK
                     DPVAMARAFALGVETGYAARQAGLMPKSDVAVATSPLTSFLK"
     gene            5704..>6683
                     /gene="thiH"
                     /locus_tag="PKI68_07040"
     CDS             5704..>6683
                     /gene="thiH"
                     /locus_tag="PKI68_07040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HNX53484.1"
                     /translation="MKNLPDWLTPEPWLNRNFSASDVQSALGAEAPDEATLAALLSPA
                     AADFLEPMAARAQALTQRHFGRTIQLYVPLYLSSYCSGGCAYCGFAADRRTDRHALDF
                     PSVEKELDAIHAMGFEEVLLLTGERSSQADLDYLRECVRLAARRFHNVTVEVFPMAEE
                     EYRSLTEAGCTGVTLYQETYHPETYDRMHRWGPKKDFLQRLDAPSRALAGGMRFAGLG
                     ALLGLADPRYDMLALYRHVRHLQKTFWQAGLAVSFPRIRNEEGGFVPPHPVDEKMLAQ
                     IIFAFRICLPDVPLVLSTREGAAFRDGMAGLGVNKMSIASKTTVGGYGSEG"
BASE COUNT         1465 a         1900 c         1839 g         1379 t
ORIGIN      
        1 ctccgacatc tgaagctatc tgattgtacc aacccgtact cgtaaagctg gccttttgcg
       61 aatagttact gcatgcgtaa gcaaaatatg ttcgtagcac ggacgggaca tcggttggat
      121 aagcagctgt cccgctagat tcaaaatctg aatccagcgc aacggccgca tgatacataa
      181 gttggccaac cgcttgtttc tgtaatgtag aactgcctgt tgtgataaga ctcggcatat
      241 tagcccagtt gtaggccgtc atccccgcat cgctgataga acggtttccc gtgcaacttc
      301 ctttgctgtc gtaatagctg tgcccggaaa gtatagcgtt tggacggctc cagtaccgca
      361 gtatttggga taccgcacac gccgtacagc cggcatagga tcgtccgccc ggaccgccaa
      421 ccgccgatgg acaataatag ttataaggat ctgcctgatc ccacgttgtg gttaggagga
      481 cagttccggc agcagcagat gtagacatag cttcaggcgc tttggtcggc aatgccggat
      541 tcaatagcgc caaccattgc tccttatatc cggatacgcc tgcactttgc gtttggctta
      601 caccagaaaa accaccgctt ttcgtttgag tcatgccgga aagcaccgac agatcctcgg
      661 ccagttccaa attgagaatg ttcaaaaact ctggcggcaa ctgatcgtag gtgccggagt
      721 ccgtgtagca ttttacggga ggtgcctcgt catcactgct caacaacaca taccctgttg
      781 aatctaactc cgcgacatag ccgatggttt gtcctccact cccgacaacc gggcggacag
      841 actgtacagc tctggctgat tgcccggccg cagtggtagt catggctgtt tgcaaccctc
      901 ccgagggata agtcagggtt ataaaagtat tagcggcctg agcggcttga tcctgtgtca
      961 caggggaacc aaccgccacc tctctaaaaa gaagaacaca tccgaatgca aagaagacgc
     1021 gccgtatctt attacccatt gcaattcctc actaattact ctctttatct gtttgaacaa
     1081 tacgataaat ccttatgtag aacacaagac gggaagtgca gtgaaaacaa cagtcagcag
     1141 gatcagactt ttttcatacg gctcccggtc agattgcgaa cacattagga aaccggaaag
     1201 agcttcgcaa gtaaacaagc cgtgaatgaa caggatggaa ctcggtttcg cgggtggaac
     1261 tacgggtttc ttctgcaggc gcggccggac tttctccgcg cccgccgtgc gcggctgcag
     1321 caggatttgc cgaaccttca ggcgctggag cactacagat gcatggtttc gcgcgccatt
     1381 ttgcggaata tccggcaccg tttgagcgct tctttggcca tcgccagatc ggctgattcg
     1441 ccgggatagc ggaattcgac tccgaagccg gaaagaaacg agaggtcgtt acggcagatt
     1501 tcccaaagcg gttcaaccgg tagcgataaa tccagcagca gaaccagatc gtgggttttc
     1561 gggaaaacga gacctgcttc tgtcagacgg cctttgaggt atttctccgc gcactgttcg
     1621 gcgtggaagc agacggcgtc ataacttgga tttttgcggg cgcggatttc ccgttccagc
     1681 agatggaagt cgcgtcctcc gctttttcga tccattcacg cgtcaggcgt ttcatagagc
     1741 acctcgcctt tttcaacgat ggtcttaaaa aacgggtcgc ccattttcag acgttcgcgg
     1801 attttgcggg gcgagcgaac cagcacatct atcgggaaaa ctgatcgtaa cgacgcacgg
     1861 atttccgccg cctgagccac ggcactgctt ttgtgattca ttacgaccag caagtcgata
     1921 tccgaatccg tcgtcggagt tccataggca taagagccaa aaagaacgat cttttcggga
     1981 tgaaatttct ccgccacttc ccgggcacac gcatgaattt tattggctga aaccatgccc
     2041 gcatggtgcc gcaaaccagt cgacaagtca attcaccctg ttagcgccgc tatgccgacc
     2101 tgttcttccg gaaattcccc aatcatctat aaatccttcc ctcaaacatt ccacggcagg
     2161 ccggaagttt gcctttatct ttaaaggggt gcgtgataaa aagccgccct atgaaaaaga
     2221 cgcttgttct cctcttcgcg ctggcggcag gctcgatcta cggggccatg ccggttgaaa
     2281 aatccatcac tcacctcagc acgccggttc agctgagtgt cgatgaatac cacagccttt
     2341 ttcctaaaga ctacggcgtc cacggtctgc gtctgaactg ctactttgcg gataaccgtt
     2401 ttatgtacgg actggatctc gggttctgga acgtttccga agatgccggc ggtctgcaga
     2461 tggcgatcta tcgcaacgaa acgcacgatt tcggcggggt tcagctttca ctctggaatg
     2521 ccgaaaccaa agacgtcggc gggattcagg tcgcgacggt tacaaccgac gcagaaaacc
     2581 tgtacggcgt tcagctgacc ggcctgctcg gacgggcgcg cgaagtgaac ggcgttcaga
     2641 tcggcgggct ttccgccgtt tccgaatcgg aaacggataa atgctggacc aaaggtttcc
     2701 aggccggcct ctacgaagcc cgcgcggaaa atctcgccgg cgttcagatc ggcggagttt
     2761 tcacagaaac cggctggtat gccaacggcg ttcagatcgg cctgctgttt aacgagtcgc
     2821 gctatacgcg cggcgttcaa ctcggcggac tgacggcgaa gtccaaagaa acccgcggcg
     2881 tgcagatcgg cggattgatg gcgaagtcgg aaatcgcggc cgacggcgtg gtgcaggcgg
     2941 ccgtcattct cgcggaaacg ggcgaaatga agggcctgca gctttccgtc gtctcggcca
     3001 atgtcatcgg caaggcggac ggcgtacagg tcggcggggt cagcaccatg gccggcgcgc
     3061 tggacggtct ggaagttgcc ggtatctgga actttgtctt tgaaaacgtc aacggagcgc
     3121 aggtcggcct gatctataac cacgcccgct atgtgcgcgg tctgcagctc ggtctgatca
     3181 accactgcga gcggctggac ggcgtacaga tcggcctgat caacaccgtt aaagaagccc
     3241 gcttcagcac ctgcccgctt ctgcgggttg atttttaggc aacttaaggc tttttttgcg
     3301 gaaaaaatcc ggcattgtcc cgcgttttca cgcgcataac cgttttctga aaggaaggat
     3361 ctatgattaa aaaaggcgga acctacgtag tgatgggcct gctcaacacc gactcgatcg
     3421 cctatgccac cggcagaatg atcgaagagc tgggcggaaa agtaatttac acggtgcaga
     3481 acgagcggct caaaaagatt ttcttcgacc gcggctgcac ggatatctcc caggaggaga
     3541 aagacgcgat ggacatccgc tattgcgatg tcaccatcga agaagaggtc cggaatctct
     3601 tcaaagaaat cggcgatctc aacggtatcg tccactccat cggcttcgcc aaccccaaga
     3661 cctgcctcgg cgaccatatt tacaccgacg cgattgagga tgtgaagcag ggctttcata
     3721 tcagctgcgc ctcgctggcc acggtttcgc agttcgcgca ggaacacatg cataagggcg
     3781 gctcgattgt caccctctcc ttcgcctctc aggtcgcctt ccccttctat aactggatgg
     3841 gcgtcaacaa ggcggcgctc gaagcgctcg tgcgcgcact ggcccgtgaa tacggacgcg
     3901 aacacgtccg cgtcaacgcc gtttccgccg ggccgctcgc caccaaagcc gccaaatcca
     3961 tcccgcactt tgcgcatctg gcgcgcgtct ggaaaaaaat gagcccgctg ccgtgggatg
     4021 tcatcaacga taaaaaggaa gtcgccagtg cggtcgtctt cctgctgggc gaaggctcca
     4081 aaaagattac cggccagacg ctctacgtgg atggcggagc ctccgttgtc ggcggcgaac
     4141 tgctgccaaa cgaacggccg atcaaagcga tgccggcaaa acgaaaagcc gccgccaaag
     4201 agcggctcga aagaatgcgg gaactgcaaa agcagcatca gcagcaggga ctgtaaaacg
     4261 aaaagccgct cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgaatggcc
     4381 tgccatgagc gagttcacga cgtgaacgag tcgaatggtg accccaacgg gaatcgaacc
     4441 cgtgttccca ggatgaaaac ctggtgtcct aaccgctgaa cgatagggcc tgaaaaagtg
     4501 agggcgtaag atagcagact ccggtgacgg ggcaacataa aataaatggt tttttgatct
     4561 atgcttttca aacccccgct ggcatcctct tttccgttcc cggggtgccg gacgccggaa
     4621 ggcggacgcg ctgagataat acccgaagaa cctgatccgg ttagtaccgg cgaaagggag
     4681 tgacgcatcg cggcggagct cccttcaaaa agggggcttt ttatgtatct gacggtaaat
     4741 gacgaacggc atgagcatcg cggcgacaga accgtccccg ctctgctggc cgaactcggc
     4801 gcaatgccgg agcacaccgc cctgaccgtg aacggcgatc tggttttctc cagagattgg
     4861 aaaacgttca aattcaatga cggggacacc gtcgaagttc ttacttttgt gggaggaggc
     4921 taggatgaac cctgaaaccg gaaccctgaa actgggcggt cacgaattta aatcgcgact
     4981 gattctcggc accggaaagt tttctgatac cgcaacaatg cttgcggctg tcgaagcctc
     5041 cggcacggag ctggtaacag ttgctttgcg ccgcttcaac cgtcaaaagc ccggcgacga
     5101 tctttgcgcg ccgctggccc ggctccaagg gatccggctg atgcccaata cctccggagc
     5161 gatgaacgcc aaagaagccg tccgcgcggc ccagctcggg cgcgaactca gcggcagccc
     5221 gttcgtaaag gtggagattc atccgaaccc gcaccacctc atgcccgatg cgatcgaaac
     5281 ctacctcgcc gccaaagagc tcgccgccga cgggtttgtc gttctgccct acattccggc
     5341 cgatccggtg ctagccaaaa aactggaaga tgtcggctgc gcggccgtca tgccgctcgg
     5401 cgcggccatc ggcagcggcg gcgggctttc taccgcggaa atgatccggc tgatcatccg
     5461 cgacagcacc attccggtca tcgtcgacgc cggactgcgc gcgccgtccg aagcggcccg
     5521 cgccatggaa atgggctgcg atgccgtact cgtgaattcc gccatcgccg ccgccaaaga
     5581 tccggtcgcg atggcgcgcg cctttgcact cggcgtcgaa accggctatg ccgcacgcca
     5641 ggcgggtctg atgccgaaaa gtgatgtggc tgtggcaacg agccctttaa cctccttttt
     5701 gaaatgaaaa atctgcctga ctggttaacg cctgagccct ggctcaaccg gaatttttct
     5761 gcatcggacg tgcaatccgc cctcggcgcg gaagcgccgg acgaagcgac gcttgccgcg
     5821 ctgctttcgc cggcggcggc ggattttctg gaaccgatgg ctgcgcgcgc gcaggcgctg
     5881 acgcaacgcc acttcggccg cacgattcag ctttatgttc cgctttatct ttccagctat
     5941 tgttccggcg gctgcgctta ctgcggcttc gccgccgacc gccgtaccga ccgccatgcg
     6001 ctggattttc cgtccgtcga aaaagagctg gatgccatcc atgcgatggg cttcgaagaa
     6061 gttctgctgc tgaccggcga gcgctcgtcg caggcggatc tcgattatct gcgcgaatgc
     6121 gtccggctgg cggcgcggcg ttttcataac gtcaccgtcg aagtttttcc gatggcggaa
     6181 gaggaatacc gctcgctgac cgaagccgga tgcaccggcg tgacgctcta tcaggaaacc
     6241 tatcacccgg aaacctatga ccgcatgcac cgctggggac cgaaaaaaga ttttctccaa
     6301 cgtctggatg ctccgtcgcg ggcgctggcg ggcggaatgc gtttcgcggg tctcggcgcg
     6361 ctgctcggcc ttgccgatcc gcgctacgac atgctcgcgc tctaccgcca cgtccgccat
     6421 ttgcaaaaaa ccttctggca ggccgggctg gccgtctcct tcccgcgtat ccgcaacgaa
     6481 gaaggcggtt ttgttccgcc gcatccggtg gacgaaaaga tgctcgcgca gattatcttc
     6541 gccttccgca tctgcctgcc ggatgttccg ctggttcttt cgacccgcga aggcgcggcg
     6601 ttccgcgacg gtatggccgg actcggtgtt aataaaatga gcatcgccag caaaaccacc
     6661 gtcggcggtt acggcagtga ggg
//
LOCUS       DAOPZO010000101         6667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_14045_length_6667_cov_9.999395, whole genome shotgun sequence.
ACCESSION   DAOPZO010000101 DAOPZO010000000
VERSION     DAOPZO010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14045_length_6667_cov_9.999395"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..523
                     /locus_tag="PKI68_07045"
     CDS             20..523
                     /locus_tag="PKI68_07045"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HNX53485.1"
                     /translation="MKSAKFLLLPVLALILSGCAGYHVGSTLPKSVKTVSLSIVNKSD
                     EPSIEVAVMKALRAELQMDGRLEVRSSETADAALTVTLTKFDLQALAFNRQKGALAEE
                     YRMTLTASVVLRDAESHEVILENPEVRGEAAFPYVADLTSAKRSALPEAAGDLARKVV
                     STTMTGW"
     gene            complement(596..862)
                     /gene="rpsT"
                     /locus_tag="PKI68_07050"
     CDS             complement(596..862)
                     /gene="rpsT"
                     /locus_tag="PKI68_07050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNX53486.1"
                     /translation="MPNIKSAKKRVRSSDKRRVGNMTVKTKVKTVRRKTREVLAAGVA
                     ATSDQAVREYSSVLDKAVKRGVIKKNTAIRRKKRAVAALRKLAK"
     gene            complement(883..1098)
                     /gene="rpsU"
                     /locus_tag="PKI68_07055"
     CDS             complement(883..1098)
                     /gene="rpsU"
                     /locus_tag="PKI68_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNX53487.1"
                     /translation="MASTTCEVKLRRGEPVEKGLRRLKKLLDREGILKEMRERRRFEK
                     PGDKRRKKQARARSRSMRDSRMPSIND"
     gene            complement(1194..1415)
                     /gene="infA"
                     /locus_tag="PKI68_07060"
     CDS             complement(1194..1415)
                     /gene="infA"
                     /locus_tag="PKI68_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNX53488.1"
                     /translation="MAKDDVLEMEGVVEKVLPATMYNVKLANGHMILAHISGKMRKNF
                     IRIAIGDRVTVEISPYDLTKGRISFRHRE"
     gene            1588..3240
                     /gene="gpmI"
                     /locus_tag="PKI68_07065"
     CDS             1588..3240
                     /gene="gpmI"
                     /locus_tag="PKI68_07065"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNX53489.1"
                     /translation="MEALKKSAVFKGPQGPVVLVIMDGVGIGKYAEGDFVLSSNTPNL
                     DWLMKNGVSTQLQAHGTAVGLPSDEDMGNSEVGHNAIGCGRVFHQGAALVNEAIASGS
                     MFAGQTWKELIANVKTNGSTLHLIGLFSDGNVHSHIDHLKAMLKQAKAEGVKTARIHP
                     LIDGRDVPPTSALDYVEPFEAFLTELNAGGAVDYAIASGGGRMNITMDRYNANWDMVK
                     RGWDTHVKGEGRLFASMKQAVETFRAEKPGILDQDLPPFVIERGGKPVGKVVDGDSVI
                     FFNFRGDRALEITKAFEADELKEFNRGPKPKVHYAGMMQYDGDLRIPRSYLVTPPAIN
                     RTMGEYLAASGVHQLAISETQKYGHVTYFFNGNRADKFNEALEDYIEIKSDVCPFEEK
                     PRMKADEITDAVVKAIESGKYEFIRLNYPNGDMVGHTGVFPAVTIAVEAVDEGLGKLK
                     AAVEKAKGIMVISADHGNADDMYEHDKTGAVKLNKAGQPQAKTAHSLNPVPAIVYDPS
                     GVSKARLAPVKGLGISSLAGTCLKLLGYEPPADYTPSIVEVG"
     gene            3312..6530
                     /locus_tag="PKI68_07070"
     CDS             3312..6530
                     /locus_tag="PKI68_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNX53490.1"
                     /translation="MHTSFPKCVLAFLSLFFFVAVSETSGRTVALTPQEQAWLAGHGG
                     TVRFASTENYPPFEFTGGNNESMGMAVEIGRWVAGAAGFQAEFFHMPLKEAREAVLSG
                     KYDVVTCLFYSKERAQDFGFTPVIFKVPASIFIPAFRADIKTLDDLNGKIIAMPERDY
                     AEEFIRMKGINCRFLPVPSFKQAVEAVATQQADAVIGDEQVVYHYLYQKGWTEKVKRV
                     GRPLYTGQCCMAVAADNPALLSILTKAVNEARSTGIIERIERKWSGLEYTPIGFQVFR
                     YIRYVVIGLGVLLAAVLLFWIWDIRLARRVLGKTEQLRNSEERLRTIFQNSPDAIFIE
                     DENGTVLDANPVACAFHKLNRSELVGRNIFDLVPAGRREETKCNFHKWFTGELKRYEG
                     LSLDGEGREVPVEVIGSPLRFEGRSAVLLLVRDMTERKQAEQALKESEMRYRGLIEAQ
                     SNFIIRIDTDGTYTFVNEAFCRFVGRARGELIGHDFRSYIYYDDIAVPERAIESLVAC
                     RERVVTVEHRMRARTRTAWVQWEDIAVFDESGRVVEIQSVGHDITERRRIYEALQESE
                     KRLRFLFEEIPHIAVQGYNAAREAIFWNRASEELYKYSKKDVLGKKIEDLVLPPDSRD
                     ETIKTFDNWVKTGTPIPPGETLKRTSDGRSVAVYSSHLATRNPRGEWEIYVIDVDLSE
                     LKRASEELVKAKEYAERASRAKSEFLANMSHEIRTPMNGVMGMTHLLLETELTAEQRD
                     SIQTIMESTKELMRIIDELLDISRIEAGEVRLNPEPFNPRETTEKVVLLFADRAGRKG
                     VDLSIAIHDSVPKQMLGDSGRIRQILINLVGNALKFTHEGHIQIRMQAERIEGGWNLL
                     ADVKDTGIGMTPELQERIFEKFTQGDTSSKREYGGAGLGLAITRQLVHLMGGEVTVAS
                     EVNKGTTFQFNLKLGCVETDKPAVETPVPDSDEKEAIAADILLVEDNLVNQKVATAML
                     KKMGCRVIIAPNGARALEQIALRKFNMIFMDCQMPVMDGFEATRAIRQMVGDIRDIPI
                     VAMTAHALKEDRQRCLDVGMNDYLAKPVHRDSLFAVLKKYCG"
     gene            6577..6664
                     /locus_tag="PKI68_07075"
     tRNA            6577..6664
                     /locus_tag="PKI68_07075"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6611..6613,aa:Ser,seq:tga)
BASE COUNT         1478 a         1806 c         1946 g         1437 t
ORIGIN      
        1 gctcttctaa ggtgtcgcca tgaaatccgc aaaattcctg ctattgcctg ttctggctct
       61 gattctctcc ggttgcgccg gttatcacgt cggcagtacg cttcccaagt cggtcaagac
      121 ggtcagtctg tcgattgtta ataaatcgga tgagccttcg attgaggtgg cggtgatgaa
      181 ggctttgcgc gccgagttgc agatggacgg gcggcttgaa gtgcggtctt cggaaacagc
      241 ggatgctgcg ctgactgtga cgctcactaa atttgatctc caggcgctgg cgtttaaccg
      301 gcagaaaggc gcgctggcag aagaataccg gatgacgctg acggcttcgg tggttttacg
      361 tgacgcagag agccatgagg tgattttgga gaatccggag gtgcgaggcg aggcggcgtt
      421 tccctatgta gccgacctga cctcggcaaa gcgcagcgcg ctgccggaag ccgcgggcga
      481 tctggcccgt aaagtcgtca gcacgacaat gaccggctgg taatcacaga atccggtgca
      541 caaaaaaagc cgtccggtga aggacggctt ttttgttttc cggcggaccg gaaaactatt
      601 tcgccagctt gcgcagagcg gcaacggccc gctttttacg gcgaatggcg gtgtttttct
      661 tgatgacgcc gcgtttgacg gctttatcaa gcaccgagct gtattcgcgt acagcctggt
      721 cggacgttgc agcaacgccg gcagccagaa cttcgcgtgt tttacggcgg acggttttga
      781 ctttcgtctt taccgtcatg ttgccgacgc ggcgcttgtc agacgaacgc actcttttct
      841 ttgcactttt aatattcggc atggttttaa cctctgtcta atttagtcgt tgatactggg
      901 catgcggctg tcacgcatgg agcgggagcg ggcgcgagcc tgtttcttgc ggcgtttgtc
      961 gcccggcttt tcaaagcggc ggcgttcgcg catttctttc aggattcctt cgcggtcgag
     1021 cagttttttc aaacggcgga gtcccttttc gacgggttcg ccgcggcgga gtttcacttc
     1081 acaagttgta ctggccataa ttttaacacc cccttaagcg ggtagaattt ttcacaaagg
     1141 cggagaagtt aggtgatgaa cgcggctttg tcaaacgatt acaccggatc ttgtcactct
     1201 ctgtggcgga atgagatacg tcctttggtc aggtcgtacg gggagatttc aacggttacg
     1261 cggtcgccga tggcgatccg gatgaagttt ttgcgcattt tgccggaaat gtgggcgagg
     1321 atcatatgtc cgtttgccag cttcacgtta tacattgtgg cgggcagaac tttttccaca
     1381 acgccttcca tttcaagaac atcatccttt gccattcatt ctccagtcgt gcagttttat
     1441 ttttgtcgcg cgccgatgct acacgcgcac cccgccattg caagaaggca tttgaaaaga
     1501 gtttccgcga aaacttgagt gcgggccgca ctcaaggcat attctgcgct cccaacccct
     1561 gtaaattttt gagagaggaa ctgcatcatg gaagcgttga aaaaatcggc tgtttttaaa
     1621 ggtcctcagg gaccggtggt cctggtcatc atggacggtg tcggtatcgg caaatatgca
     1681 gagggcgatt tcgtcctctc gtcaaacacg cccaacctcg actggctgat gaagaacggc
     1741 gtttcgaccc agcttcaggc gcacggcacc gccgtcggtc tgccgagcga tgaagatatg
     1801 ggcaacagcg aagtcggcca caacgccatc gggtgcggac gtgttttcca tcagggcgcg
     1861 gcgcttgtga acgaagcgat cgcctccggt tcgatgtttg ccgggcagac ctggaaagag
     1921 ctgatcgcca acgtgaaaac caacggctcc acactccact tgatcggact gttttccgac
     1981 ggcaatgtgc acagccacat cgaccatcta aaggcgatgc tcaagcaggc caaggccgaa
     2041 ggcgtcaaaa ccgcgcgcat tcatccgctg atcgacggac gcgatgttcc gccgacgtcc
     2101 gcgctggact atgtcgagcc gttcgaagct tttctgaccg aactcaacgc gggcggagcc
     2161 gtggattatg ccatcgcttc cggcggcgga cgcatgaaca ttacgatgga ccgctacaac
     2221 gccaactggg atatggtcaa acgcggctgg gatacgcacg tgaaaggcga gggccgtctg
     2281 tttgcttcga tgaagcaggc tgtcgaaact ttccgcgctg aaaagcccgg cattctcgac
     2341 caggatctgc cgccgttcgt gatcgaacgc ggcggcaaac cggtcggtaa agtggtggac
     2401 ggcgacagcg tgatcttttt caacttccgc ggcgaccgcg cgctggaaat cacgaaagcg
     2461 tttgaagcgg acgagctcaa ggaattcaat cgcgggccga agccgaaggt gcattacgcc
     2521 ggtatgatgc agtacgacgg cgacctgcgc attccgcgca gctatctcgt caccccgccg
     2581 gcgatcaacc gcacgatggg cgaatatctg gccgcttccg gcgtgcacca gctggcgatc
     2641 agcgaaacgc agaagtacgg ccatgtcacc tatttcttca acggcaaccg cgccgataaa
     2701 ttcaatgaag cgctcgaaga ctacatcgaa atcaaatccg acgtctgtcc gttcgaggaa
     2761 aaaccgcgca tgaaagccga cgaaatcacc gatgccgtgg tcaaggcgat cgagagcggc
     2821 aaatatgaat tcatccgcct gaactatccc aacggcgaca tggtcggcca caccggcgtc
     2881 ttccccgcgg tcacaatcgc ggttgaagcg gtggacgaag gtctgggcaa gttgaaggcc
     2941 gccgttgaga aggccaaggg catcatggtg atcagcgccg accacggcaa cgcggacgac
     3001 atgtatgagc acgacaaaac cggcgccgtg aagctcaaca aggccggaca gccgcaggcg
     3061 aagacggctc actcgctcaa cccggttccg gcgattgtct acgatccgtc cggcgtttcg
     3121 aaagcgcggc ttgcgccggt gaaaggactc gggatcagca gtctggcggg cacctgtctg
     3181 aagctgctcg gctatgagcc gccggcggat tacacgccgt cgatcgtcga ggtcggctga
     3241 gaagttgcga tcggttgaaa acgggagctt ccggctcgac gcccgacggg tcgaaacggt
     3301 aatttgccgc catgcatacg tcttttccca aatgtgtttt ggctttttta tctctctttt
     3361 tctttgttgc tgtttcagaa acctcggggc ggactgttgc tctgacacct caagaacagg
     3421 cgtggctggc cgggcacggc gggacggttc gttttgccag tacggaaaat taccctcctt
     3481 ttgaatttac cggcggaaac aacgagtcca tgggaatggc tgtcgagatc gggcgctggg
     3541 tggccggggc ggccggattc caggccgagt ttttccacat gccgctcaag gaggcccgcg
     3601 aagcggttct gagcggaaag tatgacgttg tcacctgtct gttctacagc aaagaacggg
     3661 cgcaggattt cggattcacg ccggttattt tcaaggttcc ggcttctatt tttattccgg
     3721 ccttccgcgc ggatattaag acgctcgacg atctgaacgg taaaatcatc gcgatgccgg
     3781 agcgggacta tgcggaagaa ttcatccgca tgaaggggat caactgccgg tttcttccgg
     3841 tgcccagctt taagcaggcg gtcgaggcgg tcgcgacgca gcaggccgat gccgtgatcg
     3901 gcgatgagca ggttgtttat cattaccttt atcagaaggg atggactgag aaggttaaaa
     3961 gagttggacg tccgctttat acagggcagt gctgcatggc tgtcgcggcg gacaatccgg
     4021 cacttctttc gattctgaca aaagcggtta atgaggcgcg cagcaccggg ataattgagc
     4081 ggattgagcg caagtggtcc gggctggaat acacaccgat cgggtttcag gttttccggt
     4141 atatccgcta cgtcgttatc ggattgggtg tgctgctggc agcggtgctg ctgttctgga
     4201 tatgggatat ccggctcgcc cggcgtgtgc ttggaaaaac ggaacagctc cgcaacagcg
     4261 aagaacggct gcgcacgatt tttcaaaact cgcccgatgc cattttcatt gaggatgaaa
     4321 acggaacggt tctggatgcc aatccggtgg cctgcgcatt ccataaactg aaccgcagcg
     4381 agctggtcgg ccggaatatc tttgatctgg tgcccgccgg ccgccgcgaa gaaacgaagt
     4441 gcaattttca taaatggttt accggcgagc tgaagcgcta cgaaggcctt tctctggacg
     4501 gtgaaggccg cgaagttcct gtggaagtga tcggctctcc gctccgcttt gaaggcagaa
     4561 gcgccgtact gctgctggtg cgcgatatga ccgaacgcaa acaggccgag caggcgctca
     4621 aagagagcga gatgcgctac cgcggactga ttgaagctca aagcaatttt atcatccgga
     4681 tcgacaccga cggcacctac acattcgtca acgaagcctt ctgccggttt gtcggccgcg
     4741 cacgcggcga actgatcggg catgacttcc ggtcctacat ttattacgac gatatcgctg
     4801 ttccggagcg ggcgattgaa tcgctggtcg cctgccggga acgggtggtg acggtggagc
     4861 accgtatgcg cgcccgcacc cgcacggcct gggtgcagtg ggaggacatc gctgtatttg
     4921 acgaatccgg ccgggttgtc gaaattcaga gcgtaggtca tgatatcacc gagcgccgcc
     4981 ggatttatga ggcgcttcag gaaagcgaaa aacgtctgcg cttcctgttt gaagaaattc
     5041 cgcacattgc cgttcagggc tacaacgccg cccgggaagc cattttctgg aaccgcgcca
     5101 gcgaagaact ctacaaatat tccaaaaaag atgtcctcgg taaaaaaatt gaagacctgg
     5161 ttctgccgcc tgacagccgc gatgaaacaa tcaaaacatt cgataactgg gtgaaaaccg
     5221 gcacgccgat tcctcccggt gaaacactca aacgtacatc ggacggccgt tccgttgcag
     5281 tttattccag ccatctggcg acccgcaacc cgcgcggcga gtgggaaata tatgtgatcg
     5341 acgtcgatct gtccgagctc aagcgcgcca gcgaagagct ggtgaaagcc aaggaatatg
     5401 ccgaacgcgc cagtcgcgcc aagagcgaat tcctggccaa catgagtcac gaaatccgca
     5461 ctccgatgaa cggcgtcatg ggaatgactc atctgctgct cgaaactgaa ctgaccgccg
     5521 agcagcgcga cagcattcag accatcatgg aatccacgaa ggaactgatg cgcatcatcg
     5581 acgaactgct cgacatttca cgcatcgaag ccggggaagt gcgcctgaat cccgaaccgt
     5641 ttaatcctcg cgaaaccact gaaaaggttg tgctgctttt cgccgaccgc gccggacgta
     5701 aaggcgtgga tctttccatt gccattcacg acagcgttcc gaaacagatg ctcggcgatt
     5761 ccggacgcat ccgtcagatc ctgatcaacc tggtcggcaa cgcgctcaaa ttcacccacg
     5821 aaggccatat tcagatacgg atgcaggctg agcggattga aggcggctgg aatctgctgg
     5881 ccgatgtgaa agataccggc atcggcatga cgccggagct gcaggaacgc atctttgaaa
     5941 aattcacgca gggcgacacc tcttccaagc gcgaatacgg cggtgccggc ctcggactgg
     6001 cgattacccg ccagctggta cacttaatgg gcggcgaagt aacggtcgcc agcgaagtaa
     6061 ataagggcac gacgttccag ttcaacctca agctcggttg tgttgagacc gataaaccgg
     6121 ctgtggaaac gccggttccg gattcagatg aaaaagaagc gatcgccgca gacattctgc
     6181 tggttgaaga caatctggtg aaccagaaag tggccacggc catgctcaaa aagatgggct
     6241 gccgcgtaat tattgcaccg aacggagccc gggctcttga gcagatcgcc ttgcggaaat
     6301 tcaacatgat tttcatggac tgccagatgc cggtcatgga cggcttcgaa gccacccggg
     6361 ctatccgtca gatggtcggc gacattcgcg acattccgat tgtggccatg accgcccacg
     6421 ccctcaaaga agaccgtcag cgctgcctgg atgtcggcat gaacgattac ctggccaaac
     6481 cggttcaccg cgactcgctg tttgcggtct tgaaaaagta ctgcggctag gcgcttgata
     6541 ttcctgcgcc gctccgtata tttccacact cactttggag aggtggcaga gtggtttaat
     6601 gcgccggtct tgaaaaccgg agagccgcaa ggttccgggg gttcgaatcc ctccctctcc
     6661 gccactt
//
LOCUS       DAOPZO010000102         6512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_14494_length_6512_cov_9.079604, whole genome shotgun sequence.
ACCESSION   DAOPZO010000102 DAOPZO010000000
VERSION     DAOPZO010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14494_length_6512_cov_9.079604"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(321..1196)
                     /gene="amrS"
                     /locus_tag="PKI68_07080"
     CDS             complement(321..1196)
                     /gene="amrS"
                     /locus_tag="PKI68_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNX53491.1"
                     /translation="MKTARYWHMQTDGLIECDLCPRRCRIAEGKRGACFVRQRTGDEL
                     IAASYGLTSGLAVDPIEKKPLYHFLPGSSVLSFGTVGCNLFCQFCQNWHLSRANRMEG
                     TRTTPEEIAAAAIRYGCESVAFTYNEPIIFLEFAVDTAKVCHARGLKTVAVTNGWIEP
                     EPRKEFYAHMDAANVDLKAFTDRFYQKLCNATLQPVLDTLVYIQKETDVHLEVTTLLI
                     PGWNDSPAEIDAMTKWAVSNLGTDVPWHFSAYRPTVQWNEAPPTPLESLLQARSIAER
                     NGLRNVHLGNVHSAV"
     gene            1258..2007
                     /gene="amrB"
                     /locus_tag="PKI68_07085"
     CDS             1258..2007
                     /gene="amrB"
                     /locus_tag="PKI68_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNX53492.1"
                     /translation="MIREPAVAGMFYPADSGELRRMIEGFLAAAEPGAHPKAIIAPHA
                     GYIYSGPIAASVYKRINPDGISRVVLIGPSHRVALRGLAASSAPAWRTPLGEVSVARP
                     NFIQISDAAHAQEHSLEVQVPFLQVVLGDFTLLPLVAGDASAEEVAEVLEKCWGGPET
                     LIVVSSDLSHYESSETARAMDSAAAKAITALNPRGLDYDNACGLVPIAGLLNLAKKKK
                     MRAELVDLRNSGDTAGSRDQVVGYGAFAFYE"
     gene            complement(2013..3431)
                     /gene="pyk"
                     /locus_tag="PKI68_07090"
     CDS             complement(2013..3431)
                     /gene="pyk"
                     /locus_tag="PKI68_07090"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNX53493.1"
                     /translation="MRNKQTKIICTLALDRCGSDFIKQLVDNGMDVVRLNSAHLDIAG
                     AEQIVANIRAVSDRVGILLDTKGPEVRTCNVEKTLFLKAGDTVEIGVNRVPENGFQVS
                     YSGFADEVPTGSRVLIDDGLIALSVEKKTADTLICTALNDGEIKNKKGVNVPGIKLDL
                     PALTEKDAEFIDWATRNEIDFIAHSFVRCREDILAIQGILDLRRSPIKIIAKIENREG
                     VDNLESILDVAHGIMVARGDLGIEIPAEEVPGVQKEMIKTCIRRIKPVITATQMLESM
                     VNNPRPTRAEVSDVANAIYDGTDAIMLSGETAYGKFPVEAVQMMSKVARTVESQKESL
                     LEVIPVHQQNAEVMPRNHLAKAAVACAAALPVKAIITSTNAGITARICASYRGRSPIL
                     ALSEKMSTVRQLSLSYGVYSSKIDMPVTTDELVKVCLLKMIEEKKIAQDDLIVFIGGG
                     HQNEAQHTNMILIETPAVLLKQ"
     gene            3520..4074
                     /gene="amrA"
                     /locus_tag="PKI68_07095"
     CDS             3520..4074
                     /gene="amrA"
                     /locus_tag="PKI68_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNX53494.1"
                     /translation="MRTEYQQLLVEIARHAISQSLSNRGEKKPGSGKFPAELMQKRAT
                     FVTLTIDGQLRGCIGMLEAVRPLAEDVAANARAAAFEDPRFPPLTKKEFEKLEIHISV
                     LSPPEEMTFLSEEDVLNQIRPGVDGLILQEGFRRGTFLPSVWEELPEKKLFWRHLKLK
                     AGLPADYWSDSLRVFRYTTEYFPA"
     gene            4093..5196
                     /locus_tag="PKI68_07100"
     CDS             4093..5196
                     /locus_tag="PKI68_07100"
                     /inference="COORDINATES: protein motif:HMM:NF024918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HNX53495.1"
                     /translation="MTTKKPVILFAINGLGMGNSTRCHVVIQRLSAEAEVHVITSGNG
                     LFFLKDRPEIASLSETLPLAYAFSGDGVNALGTLAGIRTMRGALREKKKRLDALLDEL
                     NPDLVVTDSEYVIRPVLQRRIPLAGINNSDVVVSEYFRRKNLPGSIRAHFWGIEYSDY
                     LLHRMKWKLAVSPSPFADKPRSDTFKRIGLIVRDGARETALERAGKPAPLPKEIDRIV
                     VMLSGSAFASQISELQTLPWKVDVIGRDGVNAGNVMYHGKIRESLELVRNAGALVING
                     GFSSVSEALAMMRPVYVIPVPNHAEQLMNALMVKEVGAGSVENTATIIQRLKEAHRTN
                     RWDGLPESRLPVSFNGGDEAADLLLGLLHGVKA"
     gene            5193..5879
                     /locus_tag="PKI68_07105"
     CDS             5193..5879
                     /locus_tag="PKI68_07105"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HNX53496.1"
                     /translation="MIICADDFGMAEDINRAITGLVHAGKITAVSCMVNMPFFTADEI
                     APLGNKVQLGLHFTLAETPQEFRSLVIRAYTRRLDRSEVYRKLSGQYGRFEKTAGFAP
                     DFIDGHLHVQQLPVVRDVLIEFVRNRCGDAPPPVRNAAMPLRQCSLKSSLISAPGCVL
                     KRKLLKVGIPTNNGFGGIYNYPDFMRYPAHLKKFLRTVPQSGIVMVHPGLDEPWRRME
                     YETLLRQDIP"
     gene            5941..>6512
                     /locus_tag="PKI68_07110"
     CDS             5941..>6512
                     /locus_tag="PKI68_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53497.1"
                     /translation="MAGLFLAAVLGGAQAGELQWYRGNTHTHTFWSDGGEFPETVADY
                     YKKQGYHFLALTDHNTFSVGERWMPLSRKGVEAAIERSVTRWGSEHLQFRAQDGTTNK
                     MVRVRPLEEVRSLVEEPGRFILIESLELTSSAAKGKQVHSNPLNVHGKKSPDLRPQDK
                     AVPIEQRLALHEQVVAQYRAAGSSPVFWSI"
BASE COUNT         1338 a         1699 c         1964 g         1511 t
ORIGIN      
        1 aaaaccgcct gatggtcgtt gaaaccgccg gagccgatgc ttcacttgct tccgccctcc
       61 ggcgcaatga aatcgtgatg cggcaggcgg atcgtttatg gattccttat tgcacacctg
      121 gcggaatgct ggatggaatt cttaagcgtt gcgggaatta gaacatttaa caattgaagt
      181 gccatgtgca catataaatt gcaaacccac cccggcctgc ggccacccct ccaatggagg
      241 ggaatggaaa cctctatacg atcccctcct ccggaggggt gccgggacgg cggggtgggt
      301 tacgtctcat ccgttaaatg ctaaacggcg ctgtggacat tgcccaggtg gacgttgcgc
      361 aggccgttgc gctcggcgat gcttctggcc tggagcagac tttccagcgg ggtcggcggc
      421 gcttcgttcc attgaaccgt cgggcggtaa gcgctgaagt gccacggcac atctgttccg
      481 aggtttgaaa cggcccattt cgtcatggcg tcaatttcgg cgggagaatc gttccatcca
      541 ggaatcaaca gggtggtcac ttcgaggtga acgtccgttt ctttctgaat ataaaccagc
      601 gtgtcgagta ccggctggag cgttgcgtta cacagctttt ggtaaaaacg gtcggtgaag
      661 gctttcagat caacgttggc ggcgtccatg tgggcataaa attctttgcg cggttccggc
      721 tcgatccagc cgttggtgac ggcgacggtt ttcagtccgc gcgcatggca gactttggcg
      781 gtatcgacgg cgaactccag aaagatgatc ggttcgttgt aggtgaacgc cacgctttcg
      841 caaccgtacc ggatcgctgc ggcggcaatc tcttccggcg tggtgcgggt tccttccatc
      901 cggttggcac ggctgaggtg ccagttctgg cagaattggc agaacagatt gcagccgacg
      961 gtgccgaacg acagcacgct ggagccgggc aggaaatggt agagcggttt cttttcaatc
     1021 ggatcgaccg ccagtccgct ggtgaggccg tacgaggccg cgatcaattc gtcgccggtc
     1081 cgctgccgaa cgaagcatgc accgcgtttt ccctccgcga tgcggcaacg gcgagggcag
     1141 agatcgcatt cgatcagccc gtcggtttgc atgtgccagt agcgcgccgt tttcatggcg
     1201 gaataataac cctgttcact tcgccgcgca tctattaaaa ttcaggccat gagagggatg
     1261 atcagagagc ccgccgtggc gggtatgttt tatccggcgg attccggcga actgcgccgg
     1321 atgattgagg gttttctggc ggcggcggag ccgggggcgc atccgaaagc gattattgcg
     1381 ccgcatgccg gttatattta ttccggcccg atcgccgcca gcgtttataa gcggatcaat
     1441 cctgacggca tttcgcgcgt tgtgctgatc ggcccgtcgc accgcgtggc gctgcgcgga
     1501 ctggcggctt ccagcgctcc ggcatggcgc acgccgctgg gtgaggtgtc ggtcgcgcgg
     1561 ccgaacttta ttcaaatcag cgatgcggcg cacgcgcagg aacacagcct cgaagttcag
     1621 gttccgtttc tgcaggtggt gcttggcgat tttactctgc tcccgctggt tgccggcgat
     1681 gcttcagccg aggaagtggc cgaggttctg gaaaaatgct ggggcgggcc ggagacgctg
     1741 attgttgtca gttccgatct cagccattac gaaagttctg aaaccgcccg cgccatggat
     1801 tccgccgccg cgaaggcgat tacggcgctc aatccgcgcg gacttgacta tgacaacgcg
     1861 tgcggactgg ttccgattgc cggactgctc aatctggcaa aaaagaaaaa gatgcgtgcg
     1921 gagctggttg acctgcgcaa ttccggcgac accgccgggt cgcgcgatca ggttgtcggc
     1981 tacggcgcct ttgcgtttta tgaataaaac ggttactgct tgagcaggac cgcgggcgtt
     2041 tcaatcagga tcatgttggt gtgctgtgct tcgttctgat gtccgccgcc gataaagacg
     2101 atgaggtcgt cctgtgcaat ttttttctct tcgatcatct tgagcaggca gactttaacc
     2161 agctcgtcag tggttacagg catgtcaatt tttgatgaat aaactccgta gctgagcgaa
     2221 agctggcgaa cggtgctcat tttttcagac agggccagaa tcggactccg gccgcggtag
     2281 gaagcacaga tacgggctgt gatgccggca tttgtgctgg tgatgatggc tttgaccggc
     2341 agcgcagccg cgcaggcgac ggccgctttg gccagatggt tgcggggcat tacttccgcg
     2401 ttctgctgat gcaccggaat gacctccagc agagattctt tctgagattc tacggtgcgg
     2461 gcgactttgg acatcatttg aacggcttcg accgggaatt ttccgtaggc ggtttcaccg
     2521 ctgagcataa tcgcatcggt gccgtcgtag atggcgttgg cgacgtcgct cacttcggcg
     2581 cgggtggggc gcggattgtt caccatggat tcaagcatct gtgtggcggt gatcaccggc
     2641 tttatccggc gaatacaggt tttgatcatc tctttctgaa cgccgggaac ttcttcggcg
     2701 ggaatttcaa tgccgaggtc gccgcgggcc accatgatgc cgtgcgcgac atccagaatg
     2761 gattccagat tatcaacgcc ttcgcggttt tcgatcttgg cgatgatttt gatcgggctg
     2821 cggcgcaggt cgagaattcc ctgaatggcc aggatgtctt cgcggcagcg gacgaacgag
     2881 tgcgcgatga aatcaatttc gttgcgcgtg gcccagtcga taaattcggc atctttttcg
     2941 gtgagagcgg gcaggtcgag cttgatgccg ggaacgttga cgcctttttt gtttttaatt
     3001 tcaccgtcat tgagcgcggt gcagatcagt gtgtcggcgg ttttcttttc gacggaaaga
     3061 gcgatgagtc cgtcatcaat cagcacccgg ctgcctgtcg gaacttcatc cgcaaagccg
     3121 ctgtaggaaa cctggaatcc attttccgga acacggttca caccgatttc gacagtatcg
     3181 ccggctttaa gaaacagtgt tttttctaca ttgcaggtgc ggacttccgg gccttttgta
     3241 tccagaagga tgccgactcg atccgaaacg gcgcggatat tggcaacaat ctgttcggcg
     3301 ccggcaatgt ccagatgtgc cgaattcagg cggactacgt ccattccatt atcgacgagc
     3361 tgcttgatga aatcgctgcc acaccggtcc agagccagcg tacagattat ctttgtctgt
     3421 ttgttgcgca taaaaaacct ttttgatggc cgtgggttgt aacagattcc gcaggcggaa
     3481 ctagctttaa atgaaaaaaa acagtaataa gacaccgata tgagaaccga atatcagcag
     3541 ttactggttg agattgcccg ccatgcgatt tcgcagtctt tgagtaatcg gggagaaaaa
     3601 aagccgggtt ccggaaaatt tccggccgaa ctgatgcaaa aacgggcgac atttgtgacg
     3661 ctgaccatcg acgggcagct gcgcggctgt atcggtatgc tggaagctgt ccgtccgtta
     3721 gcggaggacg tcgcggcaaa cgcccgcgcc gctgcttttg aggatccgcg ctttccgccg
     3781 cttacaaaaa aggaatttga aaaactggag atccatattt ccgttctttc tccgccggag
     3841 gaaatgacgt ttttatccga agaagatgtt ttaaatcaaa tccgtcccgg cgtggacggg
     3901 ttgattttgc aggaaggatt ccgccgcggc acgttcctgc cgagtgtctg ggaggaactg
     3961 ccggagaaaa aacttttctg gagacacctg aagctgaagg cggggctgcc ggcggattac
     4021 tggtcggact ctttgcgggt tttccgctat acaaccgaat acttccctgc gtagcatggc
     4081 gctatacttc ctatgacaac aaagaaaccg gtcattctgt ttgcgatcaa cggactcggc
     4141 atgggcaaca gtacgcgctg tcatgttgtt attcagcgcc tgtccgctga agccgaggtt
     4201 catgtgatca cttccggcaa cggacttttc tttttaaaag accgcccgga aatcgcctct
     4261 ctttccgaaa cccttccgct ggcctatgcg ttttccggag acggtgtgaa tgcgctcggc
     4321 acgctggcgg gtatccgtac gatgcgcggc gcattgcgcg aaaaaaagaa gcggcttgat
     4381 gcgctgctgg atgagctgaa tcccgatctg gtcgtaaccg actccgaata tgtgatccgt
     4441 cctgttcttc agcgccgcat tccgctggcc ggtattaata actccgatgt ggtggtatcg
     4501 gaatacttcc gccgcaaaaa cctgccgggt tccatccgcg cgcatttctg gggaatcgag
     4561 tattccgact atctgctgca ccgcatgaaa tggaagctgg cggtcagtcc gtcgccgttt
     4621 gctgataaac cgcgcagcga caccttcaaa cgcatcgggc tgattgtgcg cgacggcgcc
     4681 cgcgaaacgg cgctcgaacg tgccggtaaa ccagcgccgc tgccgaaaga gattgatcgc
     4741 atcgtcgtta tgctgagcgg ctcggcattt gcgtcgcaga tttctgagct tcaaacgctt
     4801 ccgtggaagg tggatgtgat cggccgtgac ggcgtcaatg ccggtaatgt tatgtatcac
     4861 gggaaaatcc gtgagagcct tgagctggta cgcaatgccg gcgcactggt cattaatggc
     4921 ggcttttcct ccgtcagcga ggctctcgcc atgatgcgcc cggtctatgt cattcctgtt
     4981 cccaatcatg cggagcagtt gatgaatgcg ctgatggtga aagaggtcgg ggcggggagt
     5041 gtggagaata cggccacgat cattcagcgc ctcaaagaag ctcaccggac gaaccgctgg
     5101 gacggccttc ctgagagccg gctgccggtc agttttaacg gtggcgatga agcggctgat
     5161 ctgctgctgg ggctgcttca cggagtaaag gcatgatcat ttgcgcggat gatttcggaa
     5221 tggcggagga tatcaaccgc gcaatcaccg ggctggtgca tgccggaaag atcacggcgg
     5281 tttcctgtat ggtcaatatg ccgtttttca cggcggacga aatcgctccg ctcggcaata
     5341 aggttcagct cggtctgcat ttcacactgg cggaaactcc gcaggaattc agaagccttg
     5401 tgatccgcgc ttacacgagg cgtctcgacc ggagtgaggt ttaccgcaag ctgtccgggc
     5461 agtacgggcg attcgaaaag accgccggtt tcgcgcctga ttttattgac ggacatttac
     5521 atgtccagca gctgccggtt gtccgcgatg tcctgattga gtttgtccgc aaccgttgcg
     5581 gtgatgcgcc gccgcctgtg cgcaacgcgg cgatgccgtt gcggcagtgc tcactgaaaa
     5641 gttctctgat ttccgcgccc ggatgtgtct tgaagcgcaa gctgctgaag gtcggaattc
     5701 cgactaataa cggcttcggc ggaatctaca actatcctga ttttatgcgt tacccggcgc
     5761 acctcaaaaa atttctgcgc actgtcccgc aaagcggaat cgttatggtg catcccggcc
     5821 ttgacgagcc atggcgccgc atggagtatg aaacactgct cagacaggat attccgtgag
     5881 ctgaggcggt cgttgcatca tgaaaataaa ggggatccga tgagagctgg acggcaatgg
     5941 atggcgggcc ttttcctggc cgctgtactg ggcggggcgc aggctggcga gttgcagtgg
     6001 tatcgtggca atacgcacac gcatacattc tggtcggacg gcggcgagtt tcctgagacg
     6061 gttgccgact attacaagaa acagggctat cactttctgg cgctcacgga tcacaatacg
     6121 ttttcagtgg gtgagcggtg gatgccgctg agtagaaaag gtgtagaagc tgccattgaa
     6181 cgcagcgtta cccggtgggg ctcggagcat ctgcaatttc gagctcagga tggcacaacc
     6241 aacaaaatgg tgcgtgtgcg gccgttggaa gaagtccggt cgctggtcga agagccgggc
     6301 cgtttcattc tgattgaaag cctggagctg acgtcaagcg cggccaaagg caagcaggtc
     6361 cattcaaacc cgctcaatgt acacggcaag aaaagtcccg atctgcggcc gcaggacaaa
     6421 gcggtgccga tcgaacaacg gctggcgctg catgagcaag tcgttgcgca ataccgtgcg
     6481 gcgggatcgt cgcccgtgtt ctggtcgatt aa
//
LOCUS       DAOPZO010000103         6299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_15250_length_6299_cov_8.720372, whole genome shotgun sequence.
ACCESSION   DAOPZO010000103 DAOPZO010000000
VERSION     DAOPZO010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15250_length_6299_cov_8.720372"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="PKI68_07115"
     CDS             <1..159
                     /locus_tag="PKI68_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNX53498.1"
                     /translation="NLQEHSMVLVRGGRVKDLPGVRYHIVRGTLDCLGVTSRRRGRSK
                     YGAKKPKA"
     gene            176..649
                     /gene="rpsG"
                     /locus_tag="PKI68_07120"
     CDS             176..649
                     /gene="rpsG"
                     /locus_tag="PKI68_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNX53499.1"
                     /translation="MARRRRAEKRTITADPRFNSELVAYMINSIMGCGKKSTAAAIVY
                     GALEDIQLKLKEEDPLTVFGRAVENIKPKLEVKSRRVGGATYQVPLEVSPKRQTALAC
                     RWLILYSKTRRGQPMRRALAMEVLDAYNNQGSAIKKRDDTHKMAQANKAFAHYKW"
     gene            665..2800
                     /gene="fusA"
                     /locus_tag="PKI68_07125"
     CDS             665..2800
                     /gene="fusA"
                     /locus_tag="PKI68_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNX53500.1"
                     /translation="MKVAVKQDGKNAKSSASAPREDVARGRTLSSIRNIGIVAHIDAG
                     KTTTSERILYYTGRVYKMGEVHEGTAVMDWMEQEQERGITITSAATTCFWKDRQINLI
                     DTPGHVDFTVEVERSLRVLDGAIGVFCGVGGVQPQSETVWRQAKKYHVPCLAFVNKMD
                     RKGADFSAVIRQMCEKLNAPAVPVQLPWGSEENFNGVIDLITMRAISFAEQSLGSEVH
                     EERIPESMAVAAEKARAELIERVSEADVQLIEAYLENPDVSAELLIAALRRATISGQI
                     VPVLCGSSLKNKGVQPLLDAVVNYLPSPLDVPAVKGIHPKTEESLSREASDFEPLSAL
                     AFKMANDPFVGKLVFVRVYSGKLEKRQNVFNPRTKKREKIGRLLRLHANSREDIEVLY
                     TGEIGAISGLKNFTTGDTLCAENDPIVLERIEFPEPVIAMAIEPKTQADRDALKEALV
                     SLTEEDPTFRVHMNEDTGQTIINGMGELHLEIIKDRIFREFKVQANAGKPVVAYRETA
                     QKSAAGEFTFSREIGGRGHFGHVVVQVAPRVRGSGNHIAFKISNEVIPAEFRDAIKDG
                     LSDALMTGVLGNYPLVDVEVTVTGGSFHPVDSSEMAFRTAAVMALRAAVGNSSPALLE
                     PIMKLEIVAPGEHLGDVMNDLNGRRGRIREIEAREAVQIVHVDVPLAEMFGYSTTLRS
                     LTKGRASYTMEPHCFEAVPDSIQETILNR"
     gene            2813..3121
                     /gene="rpsJ"
                     /locus_tag="PKI68_07130"
     CDS             2813..3121
                     /gene="rpsJ"
                     /locus_tag="PKI68_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNX53501.1"
                     /translation="MNNQRIRIRLKSFDHRVLDASSKEIVETAKRTGARVAGPIPMPT
                     RIERFTVNRSPFVNKKSMEQFEIRTHKRLLDIIDPTIKTVDELKKLNLPAGVDITIKI
                     "
     gene            3130..3759
                     /gene="rplC"
                     /locus_tag="PKI68_07135"
     CDS             3130..3759
                     /gene="rplC"
                     /locus_tag="PKI68_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNX53502.1"
                     /translation="MKSLIGKKLGMTQIFDEEGRRVPVTVIEAGPCSVVQRKTTGTDG
                     YEAVQLGFGSQKPERVAKPLAGHFKKAGVETMRELREVRVDADDAAKAGDTVTAAVFE
                     GVNFIDVLGVTKGKGFQGVMKRHNFGGGRATHGSHFHRTTGSIGMKEKPGRVLKGKKM
                     AGQMGSTEVTVQNVKVIQVRAAENLILVKGQVPGANGTTLVLREALKRK"
     gene            3775..4398
                     /gene="rplD"
                     /locus_tag="PKI68_07140"
     CDS             3775..4398
                     /gene="rplD"
                     /locus_tag="PKI68_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNX53503.1"
                     /translation="MSTLPLKNCKGKKLGEVTLSDKFTVADKGAQAVHQAVITHQAHQ
                     HQGSASTLGKGAVAGSNKKPWKQKGLGRARAGYRQSPVWRGGGVAFGPHPHKVRKQVP
                     RKVARLAFARAFGEKAESGQILVVDDFKLAAPRTKEICSVLQALEIKGKILLILEAMD
                     NSVLLAARNLQDVEVVCAGNVNTYQIVRYPQIVITKAGVEKLEQRLA"
     gene            4412..4696
                     /gene="rplW"
                     /locus_tag="PKI68_07145"
     CDS             4412..4696
                     /gene="rplW"
                     /locus_tag="PKI68_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015450970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNX53504.1"
                     /translation="MKDVYKVIQKVVLTEKGARLSEAENKYIFNVRPDANKAEIKQAV
                     EELFKVKVSAVNTLKRQGKNKRERTSSAGRTAGVKRAVVTLAEGSKIDFV"
     gene            4708..5535
                     /gene="rplB"
                     /locus_tag="PKI68_07150"
     CDS             4708..5535
                     /gene="rplB"
                     /locus_tag="PKI68_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNX53505.1"
                     /translation="MALKKYKPTTPSRRFMVTSAFDEITKSTPERSLIRAKKSTGGRN
                     SEGRISTRHRGGGHKRRYRLIDFKRNKYGIPATVVSIEYDPNRSARIALLNYADGEKR
                     YMIAPVGLKVGTVVLSGPEAEPAVGNALPLEKIPLGMTVHNIELSTGCGGQIARSAGA
                     GAQLMSREGEFANLKMPSGEIRRVRINCYATVGQVGNVDHENVSSGKAGRKRWLGIRP
                     TVRGVAMNPVDHPMGGGEGRTSGGGHPQSPWGTLAKGKRTRKKKLASNKYIIARRKK"
     gene            5539..5820
                     /gene="rpsS"
                     /locus_tag="PKI68_07155"
     CDS             5539..5820
                     /gene="rpsS"
                     /locus_tag="PKI68_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNX53506.1"
                     /translation="MARSLKKGPFVDGKLLKKIGQMEVSGRKKPIKTWARSSMIAPEF
                     VGHNFTVHNGKVFVAVFVTENMVGHRLGEFAPTRSFKSHGVATDKTVAK"
     gene            5917..6252
                     /gene="rplV"
                     /locus_tag="PKI68_07160"
     CDS             5917..6252
                     /gene="rplV"
                     /locus_tag="PKI68_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNX53507.1"
                     /translation="MEVSATTKYVRMSPIKARFMASSIRGLSVAEALRITSFNAMKAA
                     AEIGKTLKSAAANAESNEGVSADKLFIKDAYVDEGPALKRSRPRARGSASPIRKRTSH
                     ITVVLTDRK"
BASE COUNT         1586 a         1528 c         1778 g         1407 t
ORIGIN      
        1 aatctgcagg aacacagcat ggtgctggtt cgcggcggaa gggtgaagga cttgccgggg
       61 gttcgttatc atattgtgcg tggcactctt gactgcctcg gcgtgaccag tcgtagacgc
      121 ggccgttcga aatacggggc gaagaaaccg aaagcataaa gaaagttttt gaaccatggc
      181 aagaagacgc agagcagaaa aacgtacgat taccgctgat ccccgcttta acagcgagct
      241 ggtggcttac atgatcaaca gcatcatggg ctgcggtaag aaatcgacag ccgccgccat
      301 tgtttatggc gcactggaag atatccagtt gaaacttaaa gaagaagatc cgctgacggt
      361 gttcggccgc gcagtggaaa acattaagcc gaagctggaa gtgaagtccc gtcgcgtcgg
      421 cggtgcaacg tatcaggttc cgcttgaagt cagcccgaag cgtcagaccg ctttggcctg
      481 ccgttggctg attctttatt ccaagacacg tcgcggtcag ccgatgcgcc gtgcgctggc
      541 gatggaagtg ctggacgcgt ataacaatca gggttccgcg atcaagaagc gcgatgacac
      601 ccacaagatg gctcaggcca acaaagcgtt tgctcactac aagtggtaat caccgggatt
      661 cgaaatgaag gttgctgtga aacaggatgg aaaaaatgcg aagagctctg cgtctgcccc
      721 ccgggaagat gtagcgcgcg gccgcactct gtcctcgatc cgcaacatcg gcatcgtcgc
      781 gcatattgat gccgggaaaa caacgacttc cgaacgcatt ctttattaca ccggtcgcgt
      841 ctataagatg ggtgaagttc atgaaggcac cgccgtcatg gactggatgg agcaggaaca
      901 ggaacgcggc attacgatca caagcgcagc gacaacctgt ttctggaaag atcgtcagat
      961 caatctgatc gacactcccg gccatgttga tttcacagtc gaagtcgaac gctccctgcg
     1021 tgtgctcgat ggcgctatcg gcgttttctg cggagtaggg ggcgttcagc ctcagtccga
     1081 aacggtttgg cgtcaggcga aaaaatatca cgttccctgt ctcgcgtttg ttaacaagat
     1141 ggatcgcaaa ggtgcggact tctccgcagt catccgtcag atgtgcgaga agctgaatgc
     1201 cccggccgtt ccggttcaat tgccttgggg ctccgaagag aattttaatg gtgtgatcga
     1261 tcttatcacc atgcgcgcaa tttcctttgc cgagcagagt cttggcagtg aagtgcatga
     1321 agagcgtatt cctgaatcga tggctgttgc agcagaaaaa gcccgcgcag aacttatcga
     1381 acgcgtttcc gaagcagacg ttcagctgat tgaagcttat ctggaaaatc cggatgtatc
     1441 tgctgagctt ctgattgctg cgttgcgtcg cgctacaatt tccgggcaga ttgtcccggt
     1501 tctctgcggt tcatctttga aaaacaaagg tgtccagcct ttgcttgatg ccgtggttaa
     1561 ttatctgccg tctccgctgg atgttccggc tgttaaagga attcatccga agacagaaga
     1621 gtctctcagc cgtgaagcca gtgatttcga gccgcttagc gctctggcgt ttaaaatggc
     1681 aaacgatccg ttcgtcggca agctggtgtt tgtccgggtt tactccggaa agcttgaaaa
     1741 gcggcagaat gttttcaatc cgcgcaccaa gaagcgtgaa aagatcggtc gcctgcttcg
     1801 ccttcatgcc aacagccgcg aagatatcga agtgttgtac accggagaaa tcggcgcgat
     1861 ttccggtctg aaaaatttca cgaccggcga tactctttgt gctgaaaatg atccgattgt
     1921 tctggagcgc attgaatttc cggagccggt tattgcgatg gcgattgagc cgaagacgca
     1981 ggcggatcgc gacgctctta aagaggctct cgtttctctg accgaagaag atccgacatt
     2041 ccgtgtgcac atgaatgaag acaccggcca gaccattatc aatggcatgg gcgagcttca
     2101 tcttgaaatc attaaagatc gtatcttccg tgaatttaaa gttcaggcta atgccggcaa
     2161 gccggttgtt gcctatcgtg aaacagctca gaaatccgcc gccggtgaat ttactttttc
     2221 ccgcgaaatc ggcggacgcg gccacttcgg acacgtggtg gtgcaggtgg ctccccgtgt
     2281 acgcggttcg ggtaatcaca ttgcctttaa gatatccaat gaagtgatcc ctgcggaatt
     2341 ccgtgatgca attaaagacg ggcttagcga tgcgctgatg accggtgtgc tcggaaacta
     2401 tccgctggtc gatgtggaag tgacggttac cggaggcagc ttccatccgg tagattcttc
     2461 cgaaatggct tttcgcacag cggctgttat ggctctgcgc gcggcagtcg gcaattcttc
     2521 gccggcgctg ctggagccga tcatgaagct tgaaattgtt gctccgggag aacatctggg
     2581 cgacgtaatg aatgatttaa acggtcgtcg cgggcggatc cgcgagatcg aggcccgcga
     2641 ggcagttcag attgtacatg tggatgtacc tttggcagaa atgttcgggt attccaccac
     2701 attgcggtca ctgaccaagg gccgggccag ttacacgatg gagccgcatt gctttgaggc
     2761 ggtaccggat tccatacagg agaccatctt aaaccggtag tagagataaa ttatgaataa
     2821 tcagcgcata cgtatacgtt taaaatcttt tgaccaccgg gtgcttgacg cctcgtcaaa
     2881 ggaaattgtc gagaccgcaa aacgcaccgg cgcccgtgtg gcaggaccga ttccaatgcc
     2941 gacccgcatt gagcgtttca ctgtcaatcg cagtccgttc gtgaataaga aatcaatgga
     3001 acagtttgag atcagaacgc acaagcgtct gcttgatatt attgacccga cgatcaaaac
     3061 agtggacgag ctcaaaaagc ttaatctgcc ggcgggtgta gacatcacca ttaaaatctg
     3121 agttaaacca tgaagagtct aattggaaaa aaactgggaa tgacccagat cttcgacgaa
     3181 gaaggccggc gggttcctgt tacagttatt gaagcgggtc cctgcagtgt cgttcagcgc
     3241 aaaacaaccg gcacagacgg ctatgaagca gtccagctcg gattcggttc acagaaaccg
     3301 gagcgtgttg caaagcctct cgccgggcac tttaagaaag ccggcgtcga aacgatgcgc
     3361 gaattgcgtg aagtccgcgt ggatgctgat gatgcagcca aagcaggcga tacggtgacg
     3421 gcggctgttt tcgaaggcgt caattttatt gacgtgctgg gcgttaccaa aggtaaaggt
     3481 ttccagggcg taatgaagcg gcataatttc ggcggtggac gcgcaactca cggttcgcac
     3541 ttccaccgga caaccggttc gatcggtatg aaagaaaagc cgggtcgcgt tcttaaaggc
     3601 aagaagatgg ctggacagat gggcagcaca gaggtcaccg tccagaatgt caaagtgatc
     3661 caggttcgcg ctgccgaaaa cctgattctg gttaaaggcc aggtgcccgg cgcaaacggc
     3721 acgacgctgg tgcttcgcga agcactcaaa aggaagtaat ttgagaggac gatcatgagt
     3781 acattgccgc ttaaaaattg taagggaaag aagctgggtg aagtgaccct cagcgataag
     3841 ttcactgtcg ctgataaagg cgctcaggcg gtccatcagg ccgttatcac ccatcaggct
     3901 catcagcatc aaggttcagc ttctacgctg ggtaaaggtg ctgttgccgg ttccaataaa
     3961 aaaccctgga agcagaaggg tcttggccgt gcacgtgccg gttatcgtca gtcgcctgtg
     4021 tggcgcggcg ggggcgtggc tttcggtccg catccgcata aagtgcgcaa gcaggttccg
     4081 cgtaaagtgg cccgtctggc ttttgcccgt gcattcggtg aaaaagctga atccggccag
     4141 attctggttg ttgacgactt caagttagcc gcgccgcgca cgaaagaaat ctgctctgtt
     4201 ttgcaggctc tggaaattaa aggaaaaatt ctcctgattc tggaagcaat ggataacagc
     4261 gttctgctgg cggcccggaa tcttcaggat gttgaagtgg tttgcgcagg aaatgtgaac
     4321 acctatcaga tcgtccgcta tccgcagatt gttatcacca aggcgggtgt tgaaaaactc
     4381 gaacagcgcc tggcctaaga agggatttcc aatgaaagac gtatataaag taatacagaa
     4441 agtcgtgctg acggaaaaag gcgcccgcct ttccgaagcg gaaaataaat acattttcaa
     4501 cgtccgtcct gatgccaaca aggccgaaat caaacaggcg gtggaagaac tcttcaaagt
     4561 gaaagtcagt gcggttaaca cactgaaacg ccaggggaag aataaacgtg agcgtacctc
     4621 cagcgccggc cggacagccg gagtgaagcg cgcagtagta acgctggccg aaggcagcaa
     4681 gattgatttc gtgtaaggag ttcagttatg gctttaaaaa aatataaacc aacaacgccg
     4741 agccgccgct tcatggtgac ctctgcgttc gatgaaatca caaaatcgac tcctgagcgg
     4801 tcgctgatcc gtgcaaagaa gagtaccggc gggcgcaaca gtgaaggacg tatttcaacc
     4861 cgtcatcgcg gcggcggaca caagcgtcgt taccgcctga tcgatttcaa acgtaataag
     4921 tacggcattc ctgcaactgt tgtttcgatc gaatacgatc cgaaccgctc agcccgcatc
     4981 gctctgctga actatgcgga cggtgaaaag cggtatatga tcgctccggt cggtctcaaa
     5041 gtcggcacgg tcgtgctgtc cggcccggaa gctgaaccgg cggttggcaa tgcactccct
     5101 ctcgaaaaaa ttccgctggg tatgacggtt cataacatcg aactgtcgac cggttgcgga
     5161 ggacagattg cccgctctgc cggcgccggc gcacagctga tgtcccgcga aggtgaattt
     5221 gctaatctga aaatgccttc cggtgaaatc cgtcgtgtgc gtatcaactg ctacgccacg
     5281 gtcggtcagg tcggcaatgt cgatcacgaa aatgtttcca gcggtaaagc cgggcgcaag
     5341 cgctggctgg gcatccgtcc gaccgttcgc ggtgtggcga tgaatcccgt cgaccatccg
     5401 atgggcggtg gtgaaggccg tacttccggc ggcggtcatc cgcagtcgcc ttggggcacg
     5461 ctggctaagg gtaaacgcac ccgcaagaaa aaactggctt cgaataagta cattatcgca
     5521 cggagaaaga agtaacctat ggctcgttca cttaaaaaag gtcctttcgt agacggaaag
     5581 ctgctcaaaa aaatagggca gatggaagtc tctggcagaa aaaagccaat caagacatgg
     5641 gctcgctctt cgatgattgc gcctgagttt gtcggacata actttactgt tcataacggc
     5701 aaggtttttg tcgcagtgtt tgtgactgaa aatatggtag gacaccgcct cggcgagttc
     5761 gctccgaccc gttcgttcaa gtcgcatggt gttgcgacag acaagacggt tgcgaaataa
     5821 aatattttct tatagaagtt ctggatttga aaatggaatg acgtatagtt gccgcctttg
     5881 cgttcaggca gcggtagcaa atgtgagagt aaaaacatgg aagtttcagc aacaacgaaa
     5941 tatgtaagaa tgtcgccgat caaggcgcgc tttatggcgt cttcgattcg cggcctgtcg
     6001 gttgcggaag cccttcgtat tacaagcttt aatgcgatga aagcggccgc cgaaatcggc
     6061 aagacgctga agtcggccgc tgctaatgcg gaaagcaacg aaggtgtatc tgccgacaag
     6121 ctttttataa aagacgccta tgtagatgag gggccggcac ttaagcggtc ccgtccgcgt
     6181 gctcgcgggt cagcctcacc tattcgtaag cgtacgtcgc atatcacagt ggttttaacg
     6241 gatcgtaaat aaggaaaggg aatcgttttg ggacagaaag taaatcctat cggactccg
//
LOCUS       DAOPZO010000104         6245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_15443_length_6245_cov_9.403069, whole genome shotgun sequence.
ACCESSION   DAOPZO010000104 DAOPZO010000000
VERSION     DAOPZO010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15443_length_6245_cov_9.403069"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="PKI68_07165"
     CDS             <1..743
                     /locus_tag="PKI68_07165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX53508.1"
                     /translation="DRKREFFQCPECDLVFVPPEFHLSAEEEKARYDFHSHSLADSGY
                     RNFLNRLFKPLETKLRQYPPRQSLRSSSPSHGGDFLNSPPPEGCPKGGVGLDGAPFQS
                     LSALIGLDFGCGREPTLSVLFEEAGFPCANYDLHYFNDPAVLETQYDFLTCSETMEHF
                     QNPRKEFERFLRLVKPGGWIGIMTQLRDEAPSFEKWFYKDDATHLCFFSKQSFQWLEK
                     RYGPQLEFHPGGVIIFQTLEKTARESHE"
     gene            complement(855..1352)
                     /gene="infC"
                     /locus_tag="PKI68_07170"
     CDS             complement(855..1352)
                     /gene="infC"
                     /locus_tag="PKI68_07170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNX53509.1"
                     /translation="MNNMIRVPEIRCIDENGEQMGIIPTRQALIRAHEAGLDLVEISA
                     TTTPPVCRIMDHGKFKFEQEKKKKEQKKKQIAAKLKEVKFHVNIGDHDYQTKLRHTHD
                     FIEGGDRVKVSLMFRGRENAHRELGFELMKRIIADCAEVATIEQAPKLMGRNIYMMIV
                     PKKTK"
     gene            complement(1430..>2517)
                     /gene="thrS"
                     /locus_tag="PKI68_07175"
     CDS             complement(1430..>2517)
                     /gene="thrS"
                     /locus_tag="PKI68_07175"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNX53510.1"
                     /translation="LIEEFWRQEHFRNGYELLYTPHVGKANLWQTSGHLDFYKEGMYA
                     PMEIDKSDYYVKPMNCPFHINIYKSDIRSYRDLPLRWAELGTVYRYEKAGVLHGLLRV
                     RGFTQDDAHIFCTPEQIEDEIKEVLRFSTFIWKTFGFESITAYLSTRPEKAVGDPARW
                     EQATASLESALKASGLPYETDIGGGAFYGPKIDLKIKDAIGREWQMSTIQFDFNLPDR
                     FDLSYIGEDGKPHRPYMVHRALFGSIERFFGILVEHYSGAFPLWLAPEQVRILPLSEE
                     QTEAAVKLAAELRQQDIRATVDRKSGKIGAKIRTAQMDKVPYMLVLGAKEVESGTVAV
                     RDRSGAQEVMTPADFVEKVKQAVQSKG"
     assembly_gap    2518..2617
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2618..2826)
                     /locus_tag="PKI68_07180"
     CDS             complement(<2618..2826)
                     /locus_tag="PKI68_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53511.1"
                     /translation="MKNDLQLETLRHSTAHVMAAAVCRLYENVLLDIGPSTEDGFYYD
                     FDLTRRLTPEDFPAIEAEMAKIVAE"
     gene            2947..5073
                     /locus_tag="PKI68_07185"
     CDS             2947..5073
                     /locus_tag="PKI68_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNX53512.1"
                     /translation="MAALTEEQLLATLEAETIPCADLLEQFDALCADGKEVRAIEWAE
                     LLKDTLTDRKLLDEAVAAYEWLALKQGTSPAISQKELLHILSTSRKEQKFIEPAFEKA
                     ASTEEAFSRLKHLRSMKKGMLCYSKTWGFGIINRVDPFYQKVEVAFEKKGDHELAFSY
                     AATGLEVLTDDHLMAIRHKTPDEFVRLIKENPAEIIRITLRSYGPLSLNLIQDHLMPD
                     VFATEADWKKFWDAARKDLKNDPLIEIPAKRTEPLRLRRKAKAYDQTWFEKFKNERDM
                     PAVFQGLDEIQAQKIQPLEAYMAATIANRLAFAVKGGSLNQPGWIAQALVYAEQLGVE
                     PEGVDCVAELTRLARRDDLPSLLEELPSRFMQAFINRLFTASETARSFLLRRLPEFGT
                     TALNETVDALIRNGAETELAEQIRDTVRRRACSPALLLWILRNEERAEGWQLTNKADL
                     AFQTIELIEQEHGGAALRAQNQLREQIEDAESLKGALAAMSDSQRRDFMRRISESPAW
                     SKLDRQSLQAKIIKLCPDLHEVVLRKTNAAPKEKKQRITSKRSYQARKDQFEHILKKE
                     IPENSKEIELARSYGDLRENSEFKFAKEKQRLLGLQAEELQKALDEVKPTDFADFPHD
                     VAGIATGVELVYADGSRETYYILGEWDQDETLHIISSQAGMAKALAGAKAGSRVKVPT
                     SSGEAVEVEIAAVTPLSEEIRNWLNG"
     gene            5076..>6245
                     /locus_tag="PKI68_07190"
     CDS             5076..>6245
                     /locus_tag="PKI68_07190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX53513.1"
                     /translation="MNGINEWIRHGFDVVMTATFALSAVYFLLTLLAILLRRRKTPSE
                     LIAPAEFPRVTVQIPTYNELAALNCAGRCLAFDYPADRLQILIGDDSNKPEISAQIDA
                     FAAEHPGLQVCRRGNNAGFKPGNLNHMLKQTAGDYILILDSDFLPEKDFLRRLVQPVI
                     KDPTLAGVQASWRITNVHQNHTTLMGAGIINVIHIVILPFIKRFAHAGVFCGSAELIR
                     KDLLIQNGGWTPGTLTEDVDYSLRVIASGGHIAYLEDLYCTCEVPYTPRDLFRQQMRW
                     AYGVMRAFMNHGRKLLLSRLTRTRTKLAAICFGGGYIMVACFILTTLFGILNIASAWG
                     LDAVGSPYTFTDMAIETAGNFLLTCGMLISSLCASFITGVSLRNVGKLALASLTIG"
BASE COUNT         1304 a         1854 c         1690 g         1297 t
ORIGIN      
        1 gcgaccggaa gcgggaattt ttccaatgtc cggaatgcga cctggttttt gtgccgccgg
       61 agtttcattt gtcggcggaa gaggagaagg cgcgctatga ttttcacagt cacagtctgg
      121 ccgattccgg ctaccggaac tttttgaacc ggctttttaa gccgttggaa acgaagctga
      181 ggcaataccc gccccggcag tcacttcgtt ccagcagccc ctcccatgga ggggattttt
      241 taaattcccc tccgccggag gggtgcccga agggcggggt gggtttggac ggcgcgccgt
      301 tccaatcctt atccgcttta atcgggctcg atttcggctg cgggcgcgag ccgacgcttt
      361 cggttctgtt cgaggaggcc ggattcccct gcgccaacta tgacctgcac tatttcaacg
      421 atccggcggt gctggagacg cagtacgatt tcctgacctg ctcggaaacg atggagcatt
      481 tccagaatcc acgcaaagag ttcgagcgtt ttctgcggct ggtgaagccc ggcggctgga
      541 tcgggattat gacgcagttg cgcgacgagg cgccgtcgtt tgaaaagtgg ttttacaaag
      601 atgacgcaac gcacctctgc tttttctcaa aacaaagttt ccagtggctg gaaaaacggt
      661 acggcccgca gctcgaattt catcccggcg gcgtcatcat tttccagaca ttggaaaaga
      721 cagcccgcga atcacacgaa taatcacgaa tttttcagga gtgctgattc gagaagattt
      781 gtgtgattcg caggttaata aaaaagccgc tccggaagtt cggagcggct ttctgctttc
      841 caagcggctg aaacctactt ggtcttcttc ggaacaatca tcatgtagat gttgcgcccc
      901 atcagtttcg gagcctgctc aatggtcgcc acttcggcgc agtcggcaat gatccgcttc
      961 atcagttcga atccgagttc gcgatgtgcg ttttcacgtc cgcggaacat aagggatact
     1021 tttacacggt cgccgccttc gataaaatca tgtgtatggc gaagcttcgt ctggtagtcg
     1081 tgatcgccga tattgacgtg gaatttaact tctttgagct tggcggcaat ctgcttcttc
     1141 ttctgctctt tcttcttctt ctcctgctca aacttgaatt tgccgtggtc catgatgcgg
     1201 cagaccggag gagtggtcgt ggcggatatt tcgacgagat cgagacccgc ttcgtgggcg
     1261 cggatcagtg cctgtcgggt cgggatgatg cccatctgtt cgccgttttc gtcgatacaa
     1321 cgaatttcgg gcacgcggat catgttgttc attctcgggc cgaactctct gcgcggccgc
     1381 tctttgtaat tcggattgct aataacttcc tcccggttgc tttctctgat cagcctttac
     1441 tctgcacggc ctgtttcact ttttcaacaa aatcagccgg cgtcattact tcctgcgcgc
     1501 cgctgcggtc gcgcacggcg acagttccac tctcaacttc tttggcaccc agcaccagca
     1561 tatagggcac tttatccatc tgggctgtcc ggatttttgc gccgatttta ccggatttac
     1621 ggtcaaccgt tgcgcgaata tcctgctgac ggagttcggc ggccagtttg acagcggctt
     1681 ccgtctgctc ttcggaaagc ggcagaatgc ggacctgttc gggcgccagc cagagcggaa
     1741 acgctccgct gtagtgctcg accagaatgc cgaagaaccg ctcgatactg ccgaacagcg
     1801 cgcggtgcac catgtagggc cggtgcggct tgccgtcttc gccgatgtag ctgaggtcga
     1861 aacggtccgg caggttgaag tcgaactgga tggtactcat ctgccactcg cggccgatgg
     1921 catctttgat cttgaggtcg atcttcggcc cgtagaacgc gccgccgccg atatccgttt
     1981 cgtacggaag ccccgacgct ttgagcgcgg attcgagcga ggccgtggcc tgctcccagc
     2041 gcgccggatc gccgacggct ttttcggggc gggtggacag ataggcggtg atgctttcga
     2101 agccgaacgt tttccagata aaggtcgaga agcggaggac ttccttgatt tcgtcttcga
     2161 tctgctccgg tgtgcagaag atgtgcgcgt cgtcctgtgt gaagccgcga acgcgcagca
     2221 gtccgtgaag cacgccggct ttttcgtagc ggtacaccgt gcccagctcg gcccagcgca
     2281 gcggcaggtc gcggtaggag cggatgtcgg atttgtaaat gttgatgtgg aacgggcagt
     2341 tcatcggctt gacgtaatag tccgacttgt cgatttccat cggggcgtac atgccctctt
     2401 tgtagaaatc gaggtggccg gaggtctgcc agaggttggc cttgccgacg tgcggggtgt
     2461 agagcagttc atagccgttg cggaaatgct cctgccgcca gaactcttca atcagcgnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntct tccgccacaa tcttcgccat
     2641 ctcggcttcg atggccggaa aatcttccgg tgtcaggcgc cgggtcagat caaagtcgta
     2701 gtaaaacccg tcctcggtcg aagggccgat gtcgagcagg acgttttcgt aaagccggca
     2761 gacggcggcg gccatcacat gggccgtgct gtggcggagc gtttcaagtt gaaggtcatt
     2821 tttcataaat aagccgcaaa ctgtaccttt acgatcagag tgtgtcaacc tgcggaaacc
     2881 tgcggttttg gctttgccga cgcgatggaa gtccgtatgg tgtcgggtca ttttggagga
     2941 aacaccatgg ccgctctgac cgaagaacaa ttgctcgcaa cccttgaagc cgaaaccatc
     3001 ccttgcgccg acctgcttga acagttcgat gccctctgcg ccgacggtaa agaagtccgc
     3061 gccatcgaat gggccgaact gctcaaagac accttaaccg accgtaagct gctcgacgaa
     3121 gccgttgccg cctacgaatg gctcgcgctc aaacaaggca cctcgcccgc catctcgcaa
     3181 aaggaactgc tgcatattct cagcaccagc cgtaaagagc agaaattcat cgagcccgcc
     3241 tttgaaaaag ccgcctccac tgaagaggct ttttcccggc tgaaacatct gcgctccatg
     3301 aaaaaaggga tgctttgcta cagcaaaaca tggggtttcg gcatcatcaa ccgcgttgac
     3361 cccttctacc agaaggttga agtggccttt gaaaagaaag gcgaccacga actcgccttc
     3421 tcctatgccg ccaccggact tgaagtgctc accgacgacc acctgatggc catccgtcac
     3481 aaaaccccgg atgagtttgt ccggctgata aaggaaaatc cggctgaaat catccggatc
     3541 actctgcgca gctacggccc gctctcactc aatctgattc aggatcacct gatgcctgac
     3601 gtattcgcca ccgaagccga ctggaagaag ttctgggatg cggcacgcaa agatctcaaa
     3661 aacgacccgc tcattgaaat ccccgccaaa cgcaccgagc cgctgcgcct gcgtcgcaaa
     3721 gccaaggcat atgaccagac ctggtttgaa aagttcaaga acgagcgcga tatgccggct
     3781 gtttttcagg ggttggacga aattcaggcg cagaagatcc agccgctgga agcctatatg
     3841 gcggcaacga tcgccaaccg cctcgctttc gccgtcaaag gcggctcgct caatcagccc
     3901 ggctggatcg ctcaggctct tgtttacgcc gaacagctcg gcgtcgaacc ggaaggcgtc
     3961 gactgcgtgg ccgaactgac ccgcctggcc cggcgcgacg accttccgtc gctgcttgaa
     4021 gagctgccct cacgattcat gcaggccttc atcaaccgtc tcttcaccgc ttccgaaacc
     4081 gcaagaagct ttctgctgcg ccgcctgccg gaattcggca caacagcgct caacgaaacc
     4141 gtcgatgcgc tgatccgcaa cggcgcggaa accgaactgg ctgaacagat ccgcgatacc
     4201 gtccgccgcc gcgcatgcag ccccgctctg ctgctctgga ttctgcgcaa tgaagaacgc
     4261 gccgagggct ggcagctgac caataaagcc gacctcgctt tccagaccat tgaactgatc
     4321 gaacaggaac acggcggcgc cgccctgcgc gcccagaatc agctccgcga acagattgaa
     4381 gatgccgaat cactgaaagg cgctctcgcc gcgatgagcg attcgcagcg gcgcgacttt
     4441 atgcggcgga tcagcgagtc gccggcctgg agcaaactcg accgccagtc gctgcaggct
     4501 aaaatcatca agctctgccc ggatctgcac gaagtcgtcc tgcgtaaaac caacgccgca
     4561 ccgaaagaga aaaagcagcg catcacctcg aagcgcagct atcaggcgcg caaagaccag
     4621 ttcgagcaca tcctcaaaaa ggaaattccg gaaaactcca aagaaatcga actcgcccgc
     4681 agctacggcg acctgcgcga aaattccgaa ttcaaattcg ccaaagaaaa acagcgcctg
     4741 ctcggcctgc aggcggaaga actgcagaaa gcgctcgacg aagtcaaacc gaccgacttt
     4801 gccgacttcc cgcatgacgt cgccggcatt gctaccggtg tcgaactggt ctacgccgac
     4861 ggcagccgcg agacatatta tattctcggc gagtgggacc aggacgaaac gctgcacatc
     4921 atttcgtcgc aggccggcat ggccaaagca ctggccggcg ccaaagccgg cagccgggtt
     4981 aaagtaccaa cctccagcgg cgaagcggtt gaagttgaaa tcgctgcagt cacaccgctc
     5041 tccgaagaga tcagaaactg gcttaatgga tgaccatgaa cggaataaat gaatggatcc
     5101 ggcacgggtt tgacgttgtc atgacggcaa cgtttgcact ctcagcggtt tacttcctgc
     5161 tgacgctcct ggcgatcctg cttcgccgcc ggaagactcc gtccgagctg atcgctccgg
     5221 cggagtttcc gcgcgtcacc gttcagattc cgacttataa cgaactggcg gcgctgaact
     5281 gcgccgggcg ctgcctggcc ttcgactacc cggcggaccg gctccagatt ctgatcggcg
     5341 acgacagcaa taaaccggaa atctctgcgc agatcgacgc ctttgccgcg gagcatccgg
     5401 gcctgcaggt ctgccggcgc ggcaacaatg ccggcttcaa gcccggcaac ctcaaccaca
     5461 tgctcaagca gaccgccggc gactacattc tcatcctcga ctccgacttc ctgccggaaa
     5521 aagatttcct gcgccgtctg gtgcagcccg tcatcaaaga tcctacgctg gccggcgtac
     5581 aggcgtcgtg gcgcatcacc aacgtccacc agaaccacac cacgctgatg ggcgcaggca
     5641 tcatcaacgt catccacatc gtcatcctgc ccttcatcaa gcggtttgcg cacgccggtg
     5701 tattctgcgg ctcggccgaa ctcatccgca aagacctgct catccagaac ggcggctgga
     5761 cccccggtac gctcaccgaa gatgtagatt attccctgcg ggtcatcgct tccggcggac
     5821 atatcgccta tctcgaagac ctgtactgca cctgcgaagt gccgtacacc ccgcgcgacc
     5881 tgttccgcca gcagatgcgc tgggcctacg gcgtcatgcg cgccttcatg aaccacggcc
     5941 gcaaactgct gctgtcgcgc ctgacacgta cgcgcaccaa gctggccgcc atctgcttcg
     6001 gtggcggcta catcatggtc gcctgcttca tcctcaccac cctcttcggc atcctcaaca
     6061 tcgccagcgc ctggggcttg gatgcggtcg gatcgccgta tacattcaca gacatggcga
     6121 tcgagaccgc cgggaacttc ctgctcacct gcggcatgct catctccagc ctgtgcgcca
     6181 gcttcattac aggcgtcagc ctgcgcaacg tcggaaaact ggcgctggct tcgctgacca
     6241 tcgga
//
LOCUS       DAOPZO010000105         6227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_15513_length_6227_cov_10.726345, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000105 DAOPZO010000000
VERSION     DAOPZO010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15513_length_6227_cov_10.726345"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1895)
                     /locus_tag="PKI68_07195"
     CDS             complement(<1..1895)
                     /locus_tag="PKI68_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX53514.1"
                     /translation="MLRLTNICKSYQMGEYTVHALKNVSLTIEHGEFIAIIGTSGSGK
                     STLMHTIGLLDRPDSGTYEIDGKNITGLSDTQTARLRNKTIGFIFQQFNLLQRASALE
                     NTNLPLLYSGKTSEGHRGKKMLELVGLGNRTHHRPNELSGGQQQRVAIARALVNNPSI
                     ILADEPTGNLDSKSSKDIMALFRTLHRQGLTIVLVTHDIEVAEHADRIITIRDGEIQE
                     DRRNETPVINEQTEEAVLELLREDSKKKNWLALFQEGTALTRQSLRALWNNKARTFLS
                     ALGILIGVAAVIAMVSLALGAKASVKEQLSRLGSNLLSIRTSSRNSQGVQLQAGEVSR
                     MVLDDATAVLQVPSVKRSAANIHGGVQLQYGSKNWASSAYGVSPDYAPMRAEVPLLGR
                     YITDEDVTLRAQVAVIGMTPLRSLFGENENPIGKTIRINRRSYEIVGVLPERGSSAFG
                     DQDDTVRIPVTTALYRLFGKRYLDGIDAEIDKTENIPQAQDDLQQLMANRHDRDPSEK
                     PFEIRNMAEIQEALSSTTKIMSILLASIAGISLIVGGIGIMNIMLVSVTERTREIGLR
                     KALGARRQDIMAQFLIEALAISLLGGLSGLGLGIGLSLAMSKGAGWPMQISSLSLIVA
                     FGFSVFIG"
     gene            complement(1901..2902)
                     /locus_tag="PKI68_07200"
     CDS             complement(1901..2902)
                     /locus_tag="PKI68_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX53515.1"
                     /translation="MKKFILFLIVLALAGGGFWFYTLHKKKAEAAAEAQKFIPAKVEL
                     RTLRRTVESTGQVSPANRLEVKSPIAGRIEKLLVDEGDKVEKGQIVAWISSTERATLL
                     DTARAKGPDEVKYWEEIYKAAPLVAPLSGTVIVRSLEPGQTITSSSAVIVIADRLIVV
                     GQVDETDIGALFPGQRVSVKLDAYPNSAFEGTVESIAYDSKMVSNVTMYEVDVLPENL
                     PDFARSGMTSTLTFLVEEHKDVPVVPTAALEYRKNKAFIRMPAKDGKEPEVRAVKTGL
                     SENSFTEIREGLAVGDEVLIPQVVRKKETSKNPFAMAAPGRGSSSGSGSRSQGGGTR"
     gene            complement(2913..>3221)
                     /locus_tag="PKI68_07205"
     CDS             complement(2913..>3221)
                     /locus_tag="PKI68_07205"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX53516.1"
                     /translation="REQSRLEAENTMLDLLNSLQTTINTYRNSYATLSVQKAQLEAAD
                     VRATVARAQYQQGLISFQDWDTIENALVTSQRSWLSSRRAAVQAEAAWKNAMGLSSIQ
                     "
     assembly_gap    3222..3321
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3623..4636)
                     /locus_tag="PKI68_07210"
     CDS             complement(3623..4636)
                     /locus_tag="PKI68_07210"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HNX53517.1"
                     /translation="MNVRNDKARAAVLRALSELDGPAGAARIAERLPGLGVRLQPRTI
                     RLYLTELDRDGLTKLNSRRKGRVITEKGRAELEHSDVVAKVGFVRGRIDTLGYRMTFR
                     GGTGAGTVIANFALVPSRDLSRVFAEIKPVYARRLGLGDRLCLIRANEMLAGRAIPGG
                     FAALGTVCSVTVNGVLMAEGIPVTSRYGGLIELRQGRPVRLVELMEYSGVTVDPLETF
                     IRAGMTDVRESAKSGSGVITASFREIPSVALDNARRIFDRLQRSGMGGILMIGRPGQP
                     LLDIPVAEGRTGFIVVGGLNPVAAVVEAGISCELKSLSGLEEFSRFSTCEETFRRYMD
                     ALR"
     gene            4918..>6227
                     /gene="gdhA"
                     /locus_tag="PKI68_07215"
     CDS             4918..>6227
                     /gene="gdhA"
                     /locus_tag="PKI68_07215"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNX53518.1"
                     /translation="MSVVKEVLEIVERRNAGEPEFLQAVREVLESLEPVMARRKDLCD
                     AKILHRLVEPERQIIFRVPWQDDKGQVQVNRGFRFEFNSAIGPYKGGLRFHPSVNASI
                     LKFLGFEQIFKNALTTLPMGGGKGGSDFDPKGKSDGEVMRFCQSFMTELQRHIGPDTD
                     VPAGDIGVGGREIGFMFGQYRRIRNEFTGVLTGKGLKWGGSLIRPEATGYGATYFAQE
                     MLKTRRTSLEGKVCTVSGSGNVAQYTVQKISHLGGKAVTLSDSAGTIVDMDGINEEKL
                     AWVMDLKNVQRGRISEYVKKFPKAQYLEGKRPWGVKCDCAFPSATQNEIDGNDAKTLL
                     DNGCFLVSEGANMPSTPEAVERFLAKKILYGPGKAANAGGVATSGLEMSQNSMRMSWS
                     REEVDEKLNTIMVNIHKNCAETAAEYGCPGNYVAGANIAGFTKVA"
BASE COUNT         1274 a         1724 c         1806 g         1323 t
ORIGIN      
        1 ccgatgaaga ccgaaaagcc gaaggcgacg attagcgaca ggctgctgat ctgcatcggc
       61 cagccggcgc ctttcgacat ggcaaggctc agtccaatac ccagtccgag gccggagagc
      121 ccgcccagca ggctgatggc cagcgcttca atcaggaact gcgccataat gtcctgacgc
      181 cgggctccga gcgccttgcg caggccgatt tcgcgggtgc gttcggtgac ggagacgagc
      241 atgatgttca taatgccgat gccgccgacg atcagcgaaa tgcccgcaat gctggcgagc
      301 aggatagaca tgattttggt agtggagctg agagcttcct gaatttcggc catgttgcgg
      361 atttcaaacg gtttttcgga cgggtcgcgg tcatgccggt tggccatgag ttgctggaga
      421 tcgtcctgcg cctggggaat attttccgtt ttatcaatct ctgcatcgat tccatcgaga
      481 tagcgtttgc cgaaaagacg gtacaaggcg gtcgtgacgg gaattctaac ggtatcgtcc
      541 tggtctccga acgcactgga accccgttcg ggaagtacgc cgacgatttc ataggatctc
      601 cggttgatgc ggatcgtttt gccgatcgga ttttcatttt ctccgaacag ggagcggagc
      661 ggagtcattc cgatcacagc aacctgtgcg cggagggtca cgtcttcgtc cgtaatatac
      721 cgtccgagca gcggcacttc ggcgcgcatc ggcgcatagt ccggcgagac gccgtacgcc
      781 gaggaggccc agtttttgga gccgtactga agctgaacgc cgccgtggat gttagccgca
      841 gagcgtttga cggacggaac ctgtaagaca gccgttgcat cgtcgagcac catccgcgag
      901 acttccccgg cctgcagctg aacgccctga ctgttgcgcg acgaggtccg gatactcagc
      961 aggttggagc cgagccgcga aagctgttct ttcaccgagg cttttgcgcc gagtgcgagc
     1021 gagaccatcg caataacggc ggcgacgccg atcaggatgc cgagcgcgga cagaaacgtg
     1081 cgggccttat tattccagag tgcgcgcagg ctctgacggg tgagcgctgt accctcctga
     1141 aaaagcgcca gccagttttt cttttttgaa tcttcgcgca gaagttcaag cacggcctct
     1201 tccgtctgct cgttgatgac cggcgtttca ttgcggcgat cttcctgaat ctcgccgtcg
     1261 cgaatggtaa tgatccggtc ggcgtgttca gccacttcaa tatcgtgcgt gacgaggaca
     1321 atggtaaggc cctgccggtg cagggtgcgg aaaagcgcca tgatatcttt ggaacttttg
     1381 gagtccagat tgccggtcgg ctcatcggcg agaatgatcg agggattgtt gacaagtgcg
     1441 cgggcgatgg cgacgcgctg ctgctgtccg ccggaaagct cattgggccg gtggtgtgtc
     1501 cggttgccga gcccgacgag ctccagcatt ttttttccgc gatgaccttc tgaagtttta
     1561 ccggaataga gcagcggcag attggtgttt tcaagcgcag aagcgcgctg gagcagatta
     1621 aactgctgga aaatgaaacc gattgtcttg ttgcgcaggc gggcggtttg cgtatcggac
     1681 agtccggtga tgtttttccc gtcgatttcg tatgttccgc tgtccggccg gtcgagcagt
     1741 ccgatggtgt gcatcagcgt ggattttccc gatccggagg tgccgatgat ggcgataaat
     1801 tcgccgtgtt caatcgtgag cgaaacattt ttcagggcgt gtacggtgta ctcgcccatc
     1861 tgatagcttt tgcagatgtt agttaagcgc agcatggctc ttatcgcgtt ccgccgcctt
     1921 gcgaacgtga accggaaccg gagctggagc cgcgccccgg agcagccatc gcaaacggat
     1981 tcttgctggt ttcttttttg cggacaacct gcggaatcaa tacttcatcg ccgaccgcaa
     2041 gaccctcgcg gatttcggtg aacgaatttt cgctgaggcc ggttttaaca gcccgcactt
     2101 ccggttcttt gccgtctttg gcgggcatgc ggataaacgc tttgttttta cggtattcga
     2161 gcgctgcggt cggcacgacc ggaacatctt tatgctcttc caccaggaag gtcagcgtgg
     2221 atgtcatgcc gctgcgggcg aagtccggca gattttccgg cagcacatca acttcataca
     2281 tggttacatt gctcaccatc tttgagtcgt aggcgatact ttcgacggtt ccttcaaagg
     2341 cggagttcgg ataggcgtcg agttttacac tcacccgctg tccggggaac agagcgccga
     2401 tgtctgtctc gtccacctgt ccgacgacga tcagccggtc ggcgatcacg atgaccgcgc
     2461 tgctggaggt gatggtctgt ccgggctcca gcgagcggac gatgactgta ccgctgagcg
     2521 gagcaaccag cggcgccgct ttataaatct cttcccagta tttcacttcg tccggacctt
     2581 tggcgcgggc tgtgtcaagc agtgtggcgc gttcggtgga gctgatccag gcgacaatct
     2641 gacctttctc aaccttatcg ccttcgtcca ccagaagctt ttcgatgcgg ccggcgatcg
     2701 gcgattttac ttccaggcgg ttggccggac ttacctggcc ggtgctttcg accgtccggc
     2761 gcagagttct aagttccact ttcgccggga taaatttctg cgcttcagcg gcggcttcgg
     2821 cctttttctt gtgcagggta taaaaccaga aaccgccgcc tgccagcgcc agcacgatga
     2881 gaaacaaaat gaattttttc ataaccgctt tcttactgaa tcgaactcag acccatcgca
     2941 tttttccatg cagcttccgc ctgaacggcc gcccgccggc tcgacagcca gctccgctgg
     3001 ctggtaacca gcgcattctc aatcgtatcc cagtcctgga aactgattaa tccctgctga
     3061 tattgcgccc gggccaccgt tgcccgcaca tcggccgctt ccagctgcgc tttctgtacg
     3121 ctcagcgttg catagctgtt acggtaggta ttgattgtgg tctgcagact gttcagcaga
     3181 tccagcatcg tattttcagc ctccagccgg gactgctcgc gnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn naccgtccgg gcaaaacaat cctgccaggt cagcgcatca
     3361 tcggcataaa gcagccctga aaaagagatc agagctgccg ccgcggaaaa acaatggagc
     3421 ttaaaaggcg acatgggggc agactatagc gcttcttttt gaaaaattac tgccaaaatt
     3481 aacttttttt tatttggcgg gattctggct gaaccggaga agctgttccg gttaatggcg
     3541 tccggcgacc cgtgcagatt cggcggacag gaactctttc cggatcaatc aattacagct
     3601 gttctgcgat gtaaattcca gttcagcgca gcgcgtccat gtagcggcga aaggtttctt
     3661 cgcaggtgct gaagcggctg aattcttcaa ggccggaaag cgatttcagt tcgcaggaaa
     3721 ttcctgcttc gaccaccgcc gcgaccggat tgagtccgcc gacgacgatg aaaccggtgc
     3781 gtccttcagc gaccggaatg tccagcagag gctgtccggg gcggccgatc atcaagatgc
     3841 cgcccatgcc ggagcgctga agccggtcaa atatacgccg cgcattgtcg agcgcgaccg
     3901 acggaatttc gcggaagctg gcggtgatga cgccgctgcc gctttttgcg ctttcgcgca
     3961 cgtcggtcat tccggcgcgg atgaatgttt caagcggatc aacggttacg cctgaatatt
     4021 ccatcagttc aacaagccgc accgggcggc cctgacgcag ttcgatgagt ccgccgtaac
     4081 gcgaggtgac cggaatacct tcggccatta agacgccgtt gacggtgacg ctgcagacag
     4141 ttccaagcgc ggcgaaccct ccgggaattg cacgccccgc gagcatttca ttggcgcgga
     4201 tcagacagag ccggtcgccc aggccgaggc ggcgcgcata aaccggtttg atttcggcga
     4261 aaacccgcga caggtcgcgc gacggaacca gcgcaaagtt ggcgatgacc gtacccgcgc
     4321 cggtgccgcc gcggaaagtc atccggtagc cgagcgtatc aatccgtccg cggacgaagc
     4381 cgactttggc gacgacgtcg gagtgttcca gttcggcccg gcccttttcg gtgatcacgc
     4441 ggcctttgcg ccggctgttc agcttggtca gcccgtcgcg gtcgagttcg gtcaggtata
     4501 aacggatcgt gcgcggctgg agccggacgc ccaggccggg cagccgttcg gcaatccgcg
     4561 ccgcgccggc cggtccgtcg agttcgctga gcgccctcaa aacggctgcg cgcgctttgt
     4621 cgtttcgaac gttcatccgt tttttgcctc catcttgtca aaaaaagtgc ggtttaaaat
     4681 ccgccgtata acctgacatg gtgtggcgcg gtatttcgct gaattgcggg attttggcgc
     4741 catgcatttt tatacatacg gcaataatgc cgtatagatt tttaaatgca actttggctt
     4801 aaaaaataac cgttgaccgg cgatggtttt tggatgaaat gaagctcttg ttgttgaatt
     4861 tatacgccgt ttgaatgccg tataagagta agccgaaaac ctgaacagga gacgaaaatg
     4921 agtgtagtaa aggaagtgct ggagattgtc gaacgccgca acgcgggcga gccggagttt
     4981 ctgcaggctg tgcgcgaagt actcgaatcg ctggagccgg tgatggcccg ccgcaaagac
     5041 ctgtgcgatg caaaaattct gcaccgcctc gtcgaacccg agcgccagat cattttccgc
     5101 gtgccgtggc aggatgataa gggacaggtt caggtcaacc gcggcttccg cttcgagttc
     5161 aacagcgcga tcggcccgta caaaggcgga ctgcgcttcc atcccagcgt caacgccagc
     5221 atcctgaagt tcctcggctt cgagcagatt ttcaaaaacg cactgactac gcttccgatg
     5281 ggcggcggca agggcggctc ggactttgat ccgaaaggca aatccgacgg tgaggtgatg
     5341 cgcttctgcc agtcgttcat gaccgaactg caacgccaca tcggccccga tacggacgta
     5401 ccggccggtg acatcggcgt cggcggacgc gaaatcggtt tcatgttcgg ccagtaccgc
     5461 cgcatccgca acgagttcac cggcgtactc accggcaagg gcctgaagtg gggcggttcg
     5521 ctgatccgtc cggaagccac cggctacggc gcgacctact tcgcgcagga aatgctcaag
     5581 acccgccgta ccagcctcga aggcaaagta tgtacggttt ccggttccgg caacgtggcg
     5641 cagtacaccg tacagaagat cagccatctc ggcggtaaag ccgttacact gtctgattca
     5701 gccggaacca tcgtggatat ggatgggatc aatgaagaaa aactggcctg ggtgatggat
     5761 ctcaagaatg tgcagcgcgg ccgcatcagc gaatacgtta agaaattccc gaaagcgcag
     5821 tacctcgaag gtaaacgccc gtggggagtg aagtgcgact gcgcgtttcc gagcgccacg
     5881 cagaacgaaa tcgacggcaa tgatgccaag acgctgctgg acaacggctg cttcctcgtc
     5941 agcgaaggcg ccaacatgcc gagcacgccg gaagccgtcg aacggttcct cgccaagaag
     6001 attctgtacg gtccgggcaa agcggccaac gccggcggcg tggccacttc cggtctcgaa
     6061 atgagccaga actcgatgcg catgagctgg agccgcgaag aagtcgacga aaagctcaac
     6121 accatcatgg tcaacatcca caaaaactgc gccgaaaccg ccgccgaata cggctgtccg
     6181 ggcaactatg tggccggcgc caacatcgcc gggttcacca aagtggc
//
LOCUS       DAOPZO010000106         6105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_15970_length_6105_cov_9.400165, whole genome shotgun sequence.
ACCESSION   DAOPZO010000106 DAOPZO010000000
VERSION     DAOPZO010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15970_length_6105_cov_9.400165"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PKI68_07220"
     CDS             <1..648
                     /locus_tag="PKI68_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNX53519.1"
                     /translation="SRDTTIPDEIETIQSAVREAFGRVDIAVVSGGLGPTSDDITRDA
                     LAGLFDCRTVMSPAGLDALHKRYAERGRAVTPAAERQALVLEGAETLINSAGAAPGER
                     LDLPESKTLFVLPGPPDEFTTIIQEHIIPWLRGQFSDAVPLEVRVLTTANISESDIVT
                     RLEEDGFQSPGVSVGFYPGGGKVEIRLSAAYGHLDALQAAEDTLQKLLRDKLVAD"
     gene            727..1137
                     /locus_tag="PKI68_07225"
     CDS             727..1137
                     /locus_tag="PKI68_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53520.1"
                     /translation="MKQKLKIAPKYFHGLLGALLVVMVVQYALLTGLVRPIHLVFQLM
                     QLFCYLLLTVTVFRGFIQRSRLAWLISQMMLAALFSFSLLFTVVSAVLSFQSEGLWLL
                     FGAALVVAVLNGLLLGVLFSLPVCDYFRPMKDDQ"
     gene            1206..2024
                     /locus_tag="PKI68_07230"
     CDS             1206..2024
                     /locus_tag="PKI68_07230"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNX53521.1"
                     /translation="MQAISQILMDEAVAAVRKAWPQAKPRLGIILGSGWGEAVSGFTV
                     KDSLPYEKIPNLGKTSVISHAGKMLYAECGGTELFIFQGRRHVYEGGGWMPVVLPVWI
                     LKQFGVQTVLLTNASGGIRDDLRPGTLMAIEDHINLLGGSPLTGPHHPAFGERFPDQT
                     AVYDHDLRQTLIASGADVSGVYVATAGPTFETPAEIRAFRALGADAVGMSTVPEAIFA
                     NALGMKTAGLSCVCNWAAGLGDEKLSGADVIRTAKETMPRMRSVIRSFVEKCVY"
     gene            2012..2668
                     /gene="can"
                     /locus_tag="PKI68_07235"
     CDS             2012..2668
                     /gene="can"
                     /locus_tag="PKI68_07235"
                     /inference="COORDINATES: protein motif:HMM:NF007756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HNX53522.1"
                     /translation="MRILNHLFENNRNWARSILAEDPDFFKRLSGQHAPEYLWIGCSD
                     SRVPANELIGLQPGEVFVHRNIANMVIHTDMNCLSVIQYAVDLLKVRHIIVCGHYGCG
                     GVEGAMDNRPQGLVDNWLRHIRDIYQKYETTLDRITDRKRRSDKLCELNVIEQVSNVC
                     HTTIVQDAWRRGQGLTVHGWIYGLEHGLLRDLHVCVSGADELSSIYRMAVDEGLEEKE
                     "
     gene            complement(2729..3541)
                     /gene="dapB"
                     /locus_tag="PKI68_07240"
     CDS             complement(2729..3541)
                     /gene="dapB"
                     /locus_tag="PKI68_07240"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNX53523.1"
                     /translation="MSIKVVIQGAAGRMGKTLIRFIQEQKVAGLELAGAIDLWDVPDI
                     GKDAGLAAGTKEAGVKLTTDLAAVAPGADVIIDFTSHFGTAGNAERIAGWNTAWVIGT
                     TGLGDAELAEVHKAAKSVPVVMAGNMSLGVNLLCNLVKEAAKVLHARGYDIEIIERHH
                     NQKKDAPSGTALMLGQAAADGAGLDLKKSQRDGRTGLPGARTKDEIGFHAVRGGDIVG
                     DHTVLMAGTGEMIELSHRATSRETFAIGALNAAAWVVQQKPGLYNMQDVLGL"
     gene            complement(3624..4835)
                     /locus_tag="PKI68_07245"
     CDS             complement(3624..4835)
                     /locus_tag="PKI68_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53524.1"
                     /translation="MKKLKSKIAEFKSRLISINKEEPLNKLSLTTIIILDIFILTLIF
                     TGLNEHTQQLTTVNEYLPDAAREIFINQEWTPINRISKLQPILLSDRNNYYYRYESPF
                     EKRMIKLMHPASRTFYQQAATLSQDKALQALFLSRQKFVKEREKSENAFNKAKESYDT
                     QLLENIVNVSRNGTNATATTARQYAQNIDLLTGEIQSTEEQINAHPGIQKLWMIISSD
                     DHERALIVSDYKRFQFWFPLKELGWQLVFLLPIFGVFYMWSIRSVKKNNSIQTLIASH
                     LLVIASLPIILKAIELVVDIIPKHFFKKLFDVLQSLHLMALWHYFVIFAAIAVGLFLV
                     YFIQRKVFNRQKVMQKRLMKGACTGCSKKLPPGAAFCPFCGSGQFDKCAACKKETPSG
                     GQHCIHCGAPH"
     gene            5060..>6105
                     /gene="rdgC"
                     /locus_tag="PKI68_07250"
     CDS             5060..>6105
                     /gene="rdgC"
                     /locus_tag="PKI68_07250"
                     /inference="COORDINATES: protein motif:HMM:NF016281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HNX53525.1"
                     /translation="MSFESGSLSFRMFYVPGELPADIHERFADDVLGSVDYLLDEEIH
                     GWVGPRHLLDRDINEGSVWPSGFLRLTLTQAQRKIPASLLRAECRMEELARMQAEHRD
                     YINRETRSEIKKQVTERLLPQMPPQLKGIDFCYDPKEKILYASALSEAQLDAFLISFG
                     KTTGVKLVPVEPVSAAWSEARCRADQWPHWSLAAEQWADCMPGREFLMWLWYMSEARG
                     GEVELPDGGKFAVLVEGPLLFDQEEQGETAIRKGEPMLSAETRAALLSGKKLRRAKLT
                     LARGEECWTCTFDADAFTFRSIKMPKTEAIDAEGRFQERMEMVDTFRQAFLGLYSTFA
                     KLRDDKAQCKLLDTG"
BASE COUNT         1293 a         1723 c         1732 g         1357 t
ORIGIN      
        1 tcgcgcgaca ccaccatacc cgacgaaatc gaaaccattc agtccgctgt gcgcgaagcc
       61 ttcggacggg ttgacattgc cgtcgtcagc ggcggactgg gcccgacctc cgacgacatc
      121 acccgcgatg cgctcgccgg actttttgac tgccggacgg taatgtcgcc tgccgggctg
      181 gatgctctgc ataaacggta tgcagaacgg ggccgggccg taacgccggc agcggagcgg
      241 caggcgctgg ttcttgaagg cgcggaaacc ctcatcaact ccgccggcgc cgcgcccggc
      301 gaacggctgg atcttccgga aagcaaaacg ctctttgttc tgcccggccc gcccgacgaa
      361 ttcacaacca ttatccaaga acacattatc ccttggctgc gcgggcagtt cagcgatgcc
      421 gtgcccctcg aagtccgtgt gctgaccact gcgaacatca gcgaatccga catcgtgacc
      481 cgtctggaag aggatggttt tcagagcccc ggagtctctg tcggatttta ccccggcgga
      541 ggaaaagttg aaatccgtct cagtgcggca tacggacatc tggatgcgct ccaggccgct
      601 gaagacaccc tgcagaagct gctccgcgat aaacttgttg cagattagat ttagcggcgc
      661 atttgagctt ggctgaacag ggcaaacgtc taagctgatt ccggaaatga tcggggagga
      721 aaacgaatga agcagaaact gaaaatcgcg ccgaaatatt tccatggcct gctgggcgcc
      781 ttgctcgtcg tgatggttgt gcagtatgcg ctcttaaccg gtctggtgcg tcccatacat
      841 ctggtttttc agctgatgca gctgttctgc tatcttctgc tgactgtgac agtattccgc
      901 ggtttcatac agcgttcacg gctggcctgg ctgatctctc agatgatgct ggcggctctg
      961 ttttcctttt cactgctgtt tacggtcgtc agcgctgtgc tgtcatttca gtccgaaggg
     1021 ctctggcttc ttttcggcgc ggctttggta gtggccgttt tgaacggcct gctgctgggc
     1081 gttctcttct cgctgccggt ctgcgactat ttccggccga tgaaagatga tcagtaaaac
     1141 ccacggcgta gaaatgctcg gtccacaggc ttgatgcttc gcgaagatcc ttttactatc
     1201 aagccatgca agcgatcagt cagattctca tggacgaagc tgttgccgcg gtccggaaag
     1261 cgtggccgca ggcgaagccg cggctcggca ttattctcgg ttccggctgg ggcgaagccg
     1321 tcagcgggtt taccgtcaag gattcgctgc cgtatgaaaa aatcccgaac ctcggaaaaa
     1381 cttctgtgat cagccacgcg ggaaagatgc tttacgcgga atgcggcgga acggaacttt
     1441 ttatttttca gggacgccgt cacgtttatg aaggcggcgg ctggatgccg gtggttctgc
     1501 cggtctggat tctcaaacag ttcggcgtgc agacggtact gctcaccaac gcctccggcg
     1561 gcatccgcga cgacctgcgg cccggcaccc tgatggcgat tgaagatcac atcaacctgc
     1621 tcggcggcag tccgctgacc ggcccgcatc atccggcgtt cggcgaacgc ttccccgatc
     1681 agaccgctgt ctacgaccac gaccttcgtc aaacgctgat tgcgtcaggc gcggatgttt
     1741 ccggcgttta tgtcgcgaca gccgggccga ccttcgaaac gcccgccgaa atccgcgcgt
     1801 tccgcgcgct cggtgccgat gccgtcggca tgtccaccgt gccggaagcg atctttgcca
     1861 atgcgcttgg aatgaaaacg gccgggcttt cctgtgtctg caactgggcg gccgggcttg
     1921 gcgatgaaaa gttgagcggc gcggacgtca tccgtacagc gaaagaaacg atgccgcgga
     1981 tgcggtcggt aatcaggagc tttgttgaaa aatgcgtata ttaaaccatc tctttgaaaa
     2041 caaccggaac tgggcgcggt cgatccttgc agaagatccg gattttttta aacggctgtc
     2101 cggccagcat gcgccggaat acctctggat cggctgttcg gacagccgtg tgccggcaaa
     2161 tgaactgatc ggccttcagc ccggcgaggt gtttgttcac cgcaacatcg ccaacatggt
     2221 gattcacacc gacatgaact gtctttccgt catccagtac gccgtggatc tgctgaaggt
     2281 gcggcatatc atcgtctgcg gacattacgg ctgcggcggc gtggaaggcg cgatggacaa
     2341 ccgtccgcag ggactggtgg acaactggct gcggcacatt cgcgacatct atcagaaata
     2401 tgaaaccacg ctggaccgga tcaccgaccg gaaacggcgc agcgataaac tctgtgagct
     2461 gaatgtcatc gaacaggttt ccaacgtctg ccataccacg atcgttcagg atgcatggcg
     2521 gcgcgggcag ggactgacgg tacacggctg gatttacggc cttgaacacg gactgctgcg
     2581 cgacctccac gtctgcgtga gcggagccga tgagctctca tcaatttacc gtatggccgt
     2641 cgacgagggg cttgaggaaa aagagtgaac ttgggcaggg acggctcgac gagccgtccg
     2701 cggcgctttc ggcgaaagcg ccttgccgct aaagccccag tacgtcctgc atgttgtaaa
     2761 gaccgggctt ctgctgaacc acccacgcgg cggcattgag cgcgccgatc gcgaacgttt
     2821 cgcggctggt ggcgcggtgc gaaagctcga tcatctcgcc ggttccggcc atcagcaccg
     2881 tgtggtcgcc gacaatgtcg ccgccgcgca ccgcgtggaa tccgatctcg tccttcgtcc
     2941 gcgcgccggg caggccggtg cggccgtcgc gttgcgactt tttcaaatcc agacctgcgc
     3001 cgtcggccgc cgcctgaccc agcatcagcg cggtaccgct cggcgcatcc tttttctggt
     3061 tgtggtgccg ctcgatgatt tcaatgtcgt agccgcgcgc atgcagcacc ttcgccgctt
     3121 ctttgacgag gttgcagagc aggttgacgc cgaggctcat attgccggcc atcaccaccg
     3181 gcacgctttt cgcggcttta tgcacttccg ccagctcggc atcgccgagg ccggtcgtgc
     3241 cgatcaccca ggcggtattc cagccggcga tgcgctcggc attgcctgcc gtgccgaagt
     3301 gggaggtgaa atcgataatc acatcggcgc ccggagcgac ggcggcgagg tcggtcgtga
     3361 gtttgacgcc ggcttctttc gtgccggcgg ccagtccggc gtctttgccg atgtccggaa
     3421 cgtcccaaag atcaatcgcg ccggccagct cgagtccggc gactttctgt tcctgaataa
     3481 agcggatcag tgttttgccc atgcggcctg ccgcgccctg aataacaact tttatactca
     3541 tagtggttct ccggacgccg tttgtaacag aagggctctc gggaaacaaa gttgttttct
     3601 aaacattgga aatcggattt tgattaatgc ggcgctccgc agtgaatgca gtgctggcct
     3661 ccggacgggg tttccttttt gcaggctgcg catttgtcga attgtccgga tccgcagaac
     3721 ggacagaatg ccgcgccggg cggaagtttc ttgctgcaac cagtacaggc acctttcatc
     3781 agccgtttct gcatgacttt ttgccggttg aatactttcc gctgaataaa ataaaccaga
     3841 aataatccga cggcgatggc ggcaaagata acaaagtagt gccaaagagc catcagatga
     3901 agcgattgca gaacatcgaa caactttttg aaaaagtgct ttgggatgat atccacgacc
     3961 agttcaattg cttttaaaat gataggcagg ctggcaatca ccagcaggtg cgaagcgatg
     4021 agcgtttgga tggagttgtt tttctttacg ctgcggatac tccacatata gaaaacgccg
     4081 aagatgggaa gcagaaaaac aagctgccat ccgagttcct tgagaggaaa ccagaactgg
     4141 aaccgtttat agtccgaaac aatcaacgct cgttcatggt catctgacga gatgatcatc
     4201 caaagttttt ggatgcccgg gtgagcatta atttgctcct ctgttgattg gatttctcca
     4261 gtgagaaggt cgatattttg agcatattga cgggctgttg tggcggtggc attcgttcca
     4321 tttcttgaga cgttgacgat attttccagc aactgtgtgt cgtaggattc tttcgctttg
     4381 ttaaaggcgt tttcactttt ctcgcgttcc ttaacaaatt tctggcggct caggaagagg
     4441 gcctgtagtg ctttgtcttg cgagagcgtt gctgcctgtt ggtaaaacgt acgacttgcc
     4501 gggtgcatga gtttgatcat tcttttttca aaaggactct catagcggta gtaatagttg
     4561 ttccggtccg acagaaggat cggttgaagt tttgagatgc ggttgatggg ggtccattcc
     4621 tgattaatga agatttcccg ggccgcatca ggcaggtact cattgactgt agtgagctgc
     4681 tgggtgtgtt cgttcaatcc ggtgaaaatc agtgtcagaa taaaaatatc gagaatgata
     4741 atggtggtta gcgacagttt gttcaaaggc tcttctttat tgatagaaat caggcggctt
     4801 ttaaactctg cgatttttga tttcagcttt ttcatgtata gcctttctgt tcagctagtc
     4861 tgacagtttt tctctggaaa gattgcgctg agcgtagcct gatcaaaata catcggcaat
     4921 gagcaaacca tgaaaattcc tggcaccgga atatagataa aaggctggcg gcgaccggag
     4981 aaaaccggaa atatgcgcgc tcaattttca ccaataacca ataaccaata tcaaataaca
     5041 aataaccaag gtcttcccca tgtcttttga aagcggctcc ctcagcttcc ggatgtttta
     5101 tgtgcccggc gaactgccgg cggatattca cgagcgcttt gccgacgacg tcctcggctc
     5161 cgttgattac ctgctcgacg aagagattca cggctgggtc ggtccgcgcc atctgctcga
     5221 ccgcgacatc aacgaaggct cggtctggcc gtcgggcttc ctgcgcctga cgctgacgca
     5281 ggcccagcgc aaaattcccg cctcgcttct gcgcgccgaa tgccgcatgg aggaactggc
     5341 ccgcatgcag gccgagcacc gcgactacat caaccgcgaa acccgcagcg aaatcaaaaa
     5401 acaggtcacc gaacggctcc tgccgcaaat gccgccgcag ctcaaaggca tcgacttctg
     5461 ctacgacccg aaagaaaaaa tcctctacgc ctccgcgctg tccgaagcac agctcgatgc
     5521 cttcctgatc agcttcggca aaaccaccgg cgtcaaactc gtaccggtcg agccggtctc
     5581 cgcagcgtgg agcgaagcgc gctgccgcgc cgaccagtgg ccgcactgga gccttgccgc
     5641 cgagcagtgg gccgactgta tgcccggccg cgaattcctg atgtggctct ggtacatgtc
     5701 cgaagcccgc ggtggagaag tcgaactccc tgacggcgga aaattcgccg tactggtcga
     5761 aggcccgctg ctgttcgatc aggaagagca gggcgaaacc gccatccgca aaggcgaacc
     5821 gatgctcagc gccgaaaccc gcgccgcgct gctcagcggt aaaaaactgc gccgcgccaa
     5881 actcacgctg gcgcgcggcg aagagtgctg gacctgcacc ttcgatgccg acgccttcac
     5941 cttccgcagc atcaaaatgc ccaagaccga agccatcgac gccgagggcc gctttcagga
     6001 acgcatggaa atggtcgaca ccttccggca ggccttcctc ggcctctaca gcaccttcgc
     6061 caaactgcgc gacgacaaag cgcagtgcaa actcctcgat accgg
//
LOCUS       DAOPZO010000107         6101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_15992_length_6101_cov_10.345848, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000107 DAOPZO010000000
VERSION     DAOPZO010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15992_length_6101_cov_10.345848"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /locus_tag="PKI68_07255"
     CDS             complement(<1..1409)
                     /locus_tag="PKI68_07255"
                     /inference="COORDINATES: protein motif:HMM:NF017835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HNX53526.1"
                     /translation="MVIPPAGYGADGGPEWPPAIEVALSHGTFGVRLYATAGGDPRAL
                     VVFGSGDGGWSAWEHTVCTWLQEANVAVAGFDFGKYSETDFNQPTIVKDMADVAAFAA
                     ERLNAKEVPVLYGGWSMGAVQAVAAAGGTNRPPNLAGLLLLSLDSRGRYGVRTPDLVG
                     ITPTGPGTFDLHEFAPAVRDLRVVQYHGTADFMAKTTWIRLLKSPHQLYLLKGMNHGF
                     DGLSPEFEPVLLQGVAWALGDDSAAALPEQGRHLRPVRTTIYGILLLMLLAGMLSRRA
                     ALLLLPASVALCGFSNILDSIIPSSLAIIDKIQEWIPLEVSQHGRFILFVSGVMLLAL
                     ARGLRRRKRVAWHMTAVILTVSAILDFTQTFNWNRSAVALVILAALFWRRKLFDARSD
                     TPSFRLGIAVAGVMFLLLAGYGATAIHGLGARGVFGNPLSWTESFHGSICTALQIKTE
                     LNELAGREASHLLNTIRLQG"
     gene            complement(1562..1738)
                     /locus_tag="PKI68_07260"
     CDS             complement(1562..1738)
                     /locus_tag="PKI68_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53527.1"
                     /translation="MNWDAIRVWAAIVLLLDAAFGLWNHERVQALVPKINVARMALIE
                     AVAALILVLLGLWL"
     gene            complement(1735..2160)
                     /locus_tag="PKI68_07265"
     CDS             complement(1735..2160)
                     /locus_tag="PKI68_07265"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNX53528.1"
                     /translation="MTQPAEASESPVSAEAQTAQVAQSASAPGAQIESAETVAGRGRT
                     GDVNWRALAVAKLRALVEREKYYPPSAQKAGYTGRFSVHVCIETDGTISGCEITERHG
                     HPMLGRAVETAMQKIRGTNIGWSLPERYEFLLPVEFELK"
     gene            complement(2523..2744)
                     /locus_tag="PKI68_07270"
     CDS             complement(2523..2744)
                     /locus_tag="PKI68_07270"
                     /inference="COORDINATES: protein motif:HMM:NF020581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HNX53529.1"
                     /translation="MLTEIIKLLKERGPMSLAELARHFQTEVPAMEGMLDVLERKGRI
                     QRLETKCSRCKGCAQVKPEDAAIFKYAEQ"
     gene            complement(2744..3022)
                     /locus_tag="PKI68_07275"
     CDS             complement(2744..3022)
                     /locus_tag="PKI68_07275"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNX53530.1"
                     /translation="MFRRFFSKRQQQRCGRRAGQGLCCAHPLSDFAQGADVVVLSNSD
                     RKTLEMGLFSGALVHVMKNRPGDTSMVVAAGESRYIISKEAAAKIQVR"
     gene            complement(3031..5187)
                     /gene="feoB"
                     /locus_tag="PKI68_07280"
     CDS             complement(3031..5187)
                     /gene="feoB"
                     /locus_tag="PKI68_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNX53531.1"
                     /translation="MKQFTIALAGNPNCGKTTLFNALTSSRGRVGNWPGVTVEHLEGE
                     YYHDGAKIGVVDLPGIYSFSAGSPDENASRNYILKEKPDAVVNIIDARNVERGLYLTA
                     QLLEMRVPLIVVLNMLDLAERRHIEVQIDHLSRHLDCPVIPMVAARKQGLDELKAAVA
                     EAAATQRVSATNVTYDEQIEKALARLVPAVASAAERVAVPARWLAVKLLERDPVAGEL
                     TIGVCDELLAAEIAKVEKHIGEDIEIVMADGRYGFVHGLALDVVRRTGELRKNFSDAI
                     DCLVLSRALGIPLFLAVMYAVFTVTITFSGPLTAWIDTAFGTLLVGRFGDWLTGIGAP
                     VWLHVVLADGVGGGIQTVATFIPPIFFMFLCLSLLEESGYMARAAFVMDRSLRAIGLP
                     GKAFIPMLVGFGCNVPGILAARTLENQRDRTLTILLNPFMSCGARLPVYALFAGVFFP
                     QIGGAVIFALYLTGILLAVASGLLFRRTILKGDATTFVMELPPYHVPRAMAIFQHTWE
                     RLKEFILRAGKIILILVLILSVLWSVGTDGSFGNENSEHSVLSAFSRAVTPLFHPMGI
                     TDGNWPATLGLITGIFGKELVVGTLNTFYGAGGADPAAAMLAAFDGQSGAFAYLLFVL
                     IYSPCVAAIAAIQRETGWKWMTFSVMYLTALAWTLATLFYQIATFAAHPAQSTIWCGA
                     ILLAGLLFVFTLKLVSRGRGGSPNRPRTARRAVPTQ"
     gene            complement(5322..5897)
                     /locus_tag="PKI68_07285"
     CDS             complement(5322..5897)
                     /locus_tag="PKI68_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNX53532.1"
                     /translation="MPLKGSQTEKNLMCAFAGESQARNRYTYYASAAKKEGFVQIADI
                     FTETADQEKEHAKRLFNLMEGGEIEIPASCFPAGPAGKTYDNLLAAAAGEEHEWTDMY
                     PSFAKTAKDEGFTLIAAVFSAIAVAEKQHDKRYKELAANIKAGKVFEREEKVVWRCRN
                     CGYLHTGTKAPVKCAACDHPQAHFEMLGENW"
     gene            6026..6100
                     /locus_tag="PKI68_07290"
     tRNA            6026..6100
                     /locus_tag="PKI68_07290"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6059..6061,aa:Arg,seq:ccg)
BASE COUNT         1382 a         1832 c         1676 g         1211 t
ORIGIN      
        1 ccctgaagcc ggattgtgtt taagagatgg gaagcctccc gtccggcaag ttcattcagt
       61 tctgttttga tctgcagggc ggtgcagatt gagccgtgaa acgactcggt ccacgataat
      121 ggattcccga aaactccgcg cgcacccaga ccatgaatgg cagtcgcccc atagcccgcg
      181 agcagcagaa acatgacgcc ggccaccgca atgccgagcc ggaaagaagg tgtatcggac
      241 cgggcatcaa acagcttgcg ccgccaaaac agcgccgcca ggatcactaa agcaacagca
      301 gaacggttcc agttaaatgt ctgcgtgaaa tccaaaattg cagataccgt cagaataact
      361 gccgtcatat gccaggccac ccgcttccgc cggcgcaaac cccgggccag cgcgagaagc
      421 atcaccccgg aaacaaacag aataaaccgt ccgtgctgac tgacttccag aggaatccac
      481 tcctgtattt tatcaatgat cgcgagcgat gacgggataa tagaatcgag aatgtttgaa
      541 aacccgcata gagctacaga agccggcaga aggagcagcg ctgcccgccg ggaaagcatt
      601 ccggccagca acataagcaa aaggattcca tagatggtcg tcctgacagg tcgcaaatgc
      661 cggccctgct ccggaagtgc ggcggcggaa tcgtctccca gcgcccaggc cacaccttgc
      721 agcagcaccg gctcaaattc gggcgacagt ccatcgaatc cgtgattcat gcctttcaac
      781 aggtataact gatgcgggga tttcagcagg cggatccatg ttgtcttggc cataaaatcc
      841 gccgtgccgt gatattgaac cactcgtaaa tctctcaccg ccggggcgaa ctcatgcaga
      901 tcgaaagttc ccggccccgt cggcgtaatc ccgacgagat ccggcgtgcg aacgccataa
      961 cgtccgcgac tgtccagact cagcagcagc aatccggcca gattcggcgg gcggttcgtt
     1021 ccgccggcag ccgcaacggc ctgaaccgca cccatactcc agccgccgta aagcaccgga
     1081 acttccttgg catttaagcg ttccgccgca aaagccgcta catcagccat gtcttttaca
     1141 atcgttggct gattaaagtc cgtctcagag tacttgccga agtcaaaccc ggctacagcg
     1201 acgttggcct cttgaagcca ggtgcaaacg gtatgctccc aggcactcca gccgccgtcg
     1261 ccggatccga aaaccaccag tgcacgcggg tcgccgcccg ccgtggcata aagcctgact
     1321 ccgaatgtgc cgtgcgaaag agcgacttct attgccggcg gccactccgg cccgccatcc
     1381 gccccatacc ctgcaggagg aatgaccata aagctcaaaa agagcactgc gccgcatgtc
     1441 cgcattaaca aggatttcac catataaatc actccgcaca tcgccggtcg gaaactttaa
     1501 taaagggaaa acggtaacat cgctaattgt taatttaagc ataaacgatg ctgcaaagca
     1561 atcacagcca cagcccaagc agaaccagaa tcagcgcggc aaccgcctca atcaaagcca
     1621 ttcgcgcaac attgattttc ggcaccagcg cctgaacccg ctcatgattc cacagcccga
     1681 acgccgcatc cagcagcaga acaatcgccg cccagactct gatcgcatcc cagttcattt
     1741 gagctcaaac tccaccggca gcaggaattc ataccgttcc ggcaaactcc acccgatgtt
     1801 cgtgccgcga atcttctgca tcgccgtttc aaccgcccgc cccagcatcg gatgcccgtg
     1861 ccgttctgtg atttcacagc cggaaatcgt gccgtccgtc tcgatgcaga catgcacact
     1921 gaaccgtccg gtatagccgg ccttctgcgc cgacggcgga taatattttt cgcgctcaac
     1981 cagagcgcga agcttcgcaa ccgccagagc gcgccagttg acgtcccccg tccggccccg
     2041 gcccgccact gtttctgccg actcaatctg agcccccggc gctgaagccg actgtgccac
     2101 ctgcgcggtc tgcgcttcgg cggaaaccgg ggattcactg gcttcggcag gttgcgtcac
     2161 cgcaggctca ggccggggct cgggctccgg tggttttggg gggtcttcta gcggtaactc
     2221 tgccggcttt ggcggcggca gaggtgatgc cggctgagca gatgaagctg cggcgggctg
     2281 caattcgttc tgctgaatat ccagaatcgt aaccgctctc gggcccatcg acggccactg
     2341 ttcgaacgca aatttccgta ccgcaaaaaa gataactgtt cccgccgcga gatgcagcag
     2401 gcagctgatc acaaacgcca gccggccgct gcgctgttta aatggacggg atgtattcac
     2461 gatgttctgc gccggagcat aggactgcgg cagagccggt tcaacctttg attgcgctgc
     2521 ggctactgtt cagcatattt gaaaatcgcg gcatcttccg gctttacctg tgcgcaccct
     2581 ttacagcgcg aacatttggt ttccagccgc tgaatgcgtc ctttgcgctc cagcacgtcc
     2641 agcatgccct ccatcgccgg aacctcggtc tgaaaatgac gcgccagctc ggcgagcgac
     2701 atcggcccgc gttcctttag cagtttgatg atttctgtga gcatcagcgt acctgaattt
     2761 ttgcggcggc ttctttggag atgatatatc ggctttcgcc ggcggcgacg accatgctgg
     2821 tatcgccggg acggtttttc atgacatgca ccagcgcgcc gctgaacagg cccatttcga
     2881 gcgttttgcg gtcgctgttg gaaagaacaa ccacatcggc gccctgcgcg aaatcactga
     2941 gcggatgggc acagcaaagc ccctgaccgg cccgccgtcc gcaccgctgc tgctgccgtt
     3001 tgctgaaaaa ccgtctgaac atattttacc tcattgggta gggaccgctc gccgagcggt
     3061 ccgcggacgg ttcggcgaac cgcccctgcc tctggaaacc agtttaagag tgaatacaaa
     3121 taaaagtccc gccagaagaa tcgcaccgca ccagattgtc gactgcgcag ggtgcgcggc
     3181 gaaagtggcg atttgataga agagcgtcgc cagagtccag gccagcgcgg tgagatacat
     3241 gacgctgaag gtcatccact tccagccggt ttcgcgctgg atggcggcaa tcgcggcgac
     3301 gcacggcgag tagatcagta caaagagaag ataggcgaac gcgccggact gaccgtcgaa
     3361 tgcggcgagc atcgcggccg caggatcggc cccgcctgcg ccgtagaagg tgttgagagt
     3421 gccgacaacc agttcttttc caaagatgcc ggtgatgagt ccaagcgtgg cgggccagtt
     3481 gccgtccgtg attcccattg gatgaaacag cggcgtcact gcacggctga aagcgctcag
     3541 aaccgagtgt tccgaatttt cgtttccgaa acttccgtct gttccgaccg accagagaac
     3601 gctcagaatc agcaccagaa tcagaataat ttttccggcg cgcaggatga attctttgag
     3661 gcgttcccag gtgtgctgga aaatcgccat ggcgcgcggc acgtgatacg gcggaagctc
     3721 catgacaaaa gtggtggcgt cgcctttcag aatggtgcgg cggaaaagca gtccgctggc
     3781 cacggccagc agaataccgg tcaggtaaag cgcaaagatc accgcgccgc cgatctgcgg
     3841 gaaaaagacg cccgcaaaaa gcgcataaac cggcaggcgc gcgccgcacg acataaacgg
     3901 attcagaaga atggtaagcg tacggtcgcg ctgattctca agcgtccgcg ccgccagaat
     3961 gcccggcaca ttacagccga agccgaccag catcggaatg aaggctttgc ccggcaggcc
     4021 gatggcccgc agactccggt ccatcacaaa cgccgcgcgc gccatatagc cggattcctc
     4081 cagcagcgac aggcagagaa acatgaaaaa gatcggcgga ataaatgtcg ctacggtttg
     4141 gatgccgccg ccaacgccat ctgccaacac cacatgcagc cagaccggcg cgccgattcc
     4201 ggtgagccag tcgccgaacc ggccgacaag cagcgtcccg aaagccgtat caatccaggc
     4261 ggtgagcggg ccggaaaagg ttatggtgac ggtgaatacc gcatacatca ccgctaggaa
     4321 aagcggtatg ccgagcgcgc ggctgagaac aaggcagtca atggcatcgg aaaaattctt
     4381 tcgaagctcg ccggtccggc gcacaacgtc gagcgcgagg ccgtgaacaa agccataacg
     4441 gccgtcggcc atgacaattt cgatgtcttc gccgatgtgt ttttcaacct tggcgatttc
     4501 cgctgcgagc agttcgtcgc agactccaat cgtcagttcc ccggcaaccg gatcgcgctc
     4561 cagcagtttg acggcgagcc agcgggccgg aacagcaacc cgctctgcgg cggatgcgac
     4621 agcaggaacc agccgggcca gtgctttttc gatctgttcg tcatacgtca cattcgtggc
     4681 gctcacccgt tgtgttgcgg cggcttcagc tacggcggct ttaagttcgt ccaatccctg
     4741 tttgcgggca gcaaccatcg gaataacagg gcaatcgaga tgccgggaca gatgatcaat
     4801 ctgcacttca atgtgccgcc gttcggctaa atccagcatg ttgagaacga cgatcagcgg
     4861 cacgcgcatt tcgagcagtt gcgccgtcag ataaagcccg cgttcgacat tgcgggcgtc
     4921 aatgatgttt acaaccgcat ccggcttttc tttgaggata taattgcgcg aggcgttttc
     4981 gtcgggcgat ccggcggaga atgaatagat gcccggcaga tccaccacgc cgattttcgc
     5041 gccgtcatgg tagtactcgc cttccagatg ttcgacggtg acgcccggcc agttgccgac
     5101 gcgtccgcgc gagctggtca gggcattaaa cagcgtcgtt ttgccgcaat tcggattccc
     5161 tgccaaagct atggtgaact gtttcattcc gcctcaattc aaaagaaatg atagaaccga
     5221 aacccgccgt tgcgagaaca aaacccgacc aacaaatacc ggaaaacagt aagggcttcc
     5281 ggttttccgg aagcccttgc tccactccgc cgggaaaccg gctaccagtt ttcgccgagc
     5341 atttcgaaat gcgcctgcgg atgatcgcat gccgcgcatt tgaccggagc tttggtgccg
     5401 gtgtgcagat agccgcagtt gcggcagcgc cagaccactt tttcttcgcg ttcaaacacc
     5461 ttgcccgctt tgatgttggc ggcgagctct ttgtagcgct tgtcgtgctg cttttcagcc
     5521 accgcgattg cgctgaaaac agccgcgatc agcgtaaacc cttcatcctt ggcggttttt
     5581 gcgaaggacg ggtacatatc ggtccattcg tgttcttcac cggcggcggc ggccagcagg
     5641 ttgtcatagg tcttaccagc cggtccggca ggaaaacaac tggcaggaat ttcaatttcg
     5701 ccgccttcca tcagattgaa aagacgcttg gcgtgttctt tttcctgatc ggcggtttca
     5761 gtgaaaatgt cggcaatctg cacgaatcct tctttttttg ctgcactggc gtagtaggtg
     5821 tagcggttgc gggcttgcga ttcgccggcg aaagcacaca tcaaattttt ctcggtctga
     5881 cttcctttaa gcggcatctc tcgtcctcct tttgggttaa cggggcgcat tatgcggctt
     5941 ccggcgcata tttcaaaaca gaattaaacg gttttggctt ttctccgcgg gcattaaccg
     6001 ttaacagata gcacgctttt tgaatgcacc tgtagttcaa tggatagaac actggcctcc
     6061 gaagccggtg atctgggttc gactcccggc aggtgcacca g
//
LOCUS       DAOPZO010000108         6063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_16135_length_6063_cov_8.992676, whole genome shotgun sequence.
ACCESSION   DAOPZO010000108 DAOPZO010000000
VERSION     DAOPZO010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16135_length_6063_cov_8.992676"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..479
                     /locus_tag="PKI68_07295"
     CDS             81..479
                     /locus_tag="PKI68_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNX53533.1"
                     /translation="MKILATVDFSTTSESILRITKTYAKKMGAEVFLVHAEPAQTAVS
                     QDEYDTKPEAMRLRRDALALEKAGVKVTSVFVEGPACDTILSEALRLKVDLIITGAHG
                     HGGANCKASVGHISECILLRSKIPVMVVPV"
     gene            723..3167
                     /gene="gyrB"
                     /locus_tag="PKI68_07300"
     CDS             723..3167
                     /gene="gyrB"
                     /locus_tag="PKI68_07300"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNX53534.1"
                     /translation="MSDQNLEQQPKKSAGYGADQITVLEGMAAVRKRPAMYIGDTGIR
                     GYHHCVYEVVDNSIDEALAGYCTHVDVTINSDGSLTVTDDGRGIPVDMHPTEHKPAVE
                     VVLTVLHAGGKFDNDSYKVSGGLHGVGVSCVNALSEWLEVEVCRNGQVYHQRFKRGAT
                     VSQLEVIGKTQKTGTKVTFKLDHDIFTHEGFQWDILASRLRELAFLNRGVRIRLAQEE
                     PQRDEVFEYEGGISEFVRHLNKGKVAIHPDVIYFERERDGITAEIAMQYSDAYNESIY
                     SFANNINTIEGGTHLSGFRSALTRTVNQYARTNKLIKDDKQAMGGDDIREGLTAVISV
                     KIPNPQFEGQTKTKLGNGEVQGVVEAIVNEELGTYFEENPSVARGLIEKAMLAARARD
                     AARKARDLTRRKGALESGGLPGKLADCSSRDPALCELYIVEGDSAGGSAKQGRDREFQ
                     AILPVKGKVINVEKARLDKVLANEEIRTMITAIGTGIGADEFDLSKTRYNTIVIMTDA
                     DVDGAHIRTLLLTFFYRQMPQLIEAGYIYIAQPPLYKIKRKKREEYIESEAHLTRILL
                     DLGSEDQTLVCPLGNTLLESAQLPEVLQLLSEVEKIAGQMVRRGMDFDDYIRRMNSDG
                     RLPSFRVEIDHGKGEPKIHYAFNDTELRALREQTETEMGKELEIADSLTGGPGFRWIE
                     IHTAERLEKLIAQLDAKGFHARCLSHLETPEFYLTDEKGEKKALHSLLELLTTIRELG
                     RRGLSIQRYKGLGEMNPEQLWETTMDPAKRKMTRVVLEDAFKADQMFTILMGDEVEPR
                     REFIEKNALNVQNLDI"
     gene            3189..5693
                     /gene="gyrA"
                     /locus_tag="PKI68_07305"
     CDS             3189..5693
                     /gene="gyrA"
                     /locus_tag="PKI68_07305"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNX53535.1"
                     /translation="MNTQQHQRIDLINIEDEMQRAYIDYSMSVIIGRALPDARDGLKP
                     GNRRILFAMKERGWNSSKPFVKCAKVVGEVIGNYHPHGDTAVYDTIVRMAQDFSMRCM
                     LINGQGNFGSIDGDRAAAYRYTECKLLPLAEEMLADIDKNTVDMRPNFDETLMEPTVL
                     PARVPNLLINGSTGIAVGMATNIPPHNLSEVIDGTIHLIENPDASVEDLCKFIKGPDF
                     PTAGIVHGLGGIKSMYLTGRGRIRMRGKADIEEFGNGRERIIITEIPYAVNKAAMITR
                     MAELVREKNLEGISDIRDESGKLGIRVVIELKKGAIGKVVLNNIYKHTQLASTFGAIM
                     LAIDNNRPRIMNLKELLQCFIKHRFEVITRRTQFDLDKSRARAHILEGLLIALDNLDE
                     VVRIIRASKAREEAQARLTERFQFSELQVKAILDMRLYQLTGLEREKIQAEYDELQKL
                     IAYLIDLLEHEDKLYGVIRDDLAEIKAKYGEPRRTQLEVDEGEIDIEDLIADEPCAIT
                     LSNTGYIKRVPVDTYRQQRRGGKGVMGMDTKDEDFVEHVFTASTHDYLLCFTQNGRMY
                     WLKAYHVPEGSRQSRGRALANVIEMGPDEKLAAILCVRELDDEQHHLLMATKNGTIKK
                     TVLAAYKNIRAGGINAIGVDEGDQLIGVQLTSGTDEIILSLRNGKAIRFNETDCRPMG
                     RTAYGVKGVTLEGDDQVVSIEIVNTAATMMAITENGYGKRTSFDEYRLQSRGGKGIIS
                     IQTTERNGKVVSAHAVTDENRIMLISQNGQMICIGASDLRVIGRNTQGVRLVNLNEGD
                     KIVSAAVLDPEAEAVETVAPVSTEVVEGDLPEADAE"
     gene            5702..6058
                     /locus_tag="PKI68_07310"
     CDS             5702..6058
                     /locus_tag="PKI68_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53536.1"
                     /translation="MSIDLSSLNLTPEWSKSTPGSSLPPKKDKPVKQPPAQPGDGIVR
                     VGRETKGRKGSGVTVIAGIPSHPEGLEKLAKQFKQLCGTGGTVKDGVIEIQGEHRDRL
                     VAELQKLGYTVKRAGG"
BASE COUNT         1494 a         1771 c         1716 g         1082 t
ORIGIN      
        1 taaataattc ttcgctcttt tccggtttac ttccctccgc ggcctgccga taatagaagc
       61 gaagaagaaa ggggtgcaca atgaaaatac tggcaactgt tgacttctcc accacttcag
      121 aaagcattct gagaatcaca aaaacctatg caaaaaaaat gggtgcagag gtttttctgg
      181 ttcacgccga acccgcacag acggctgtca gtcaggacga atacgacacg aagcctgaag
      241 ccatgcgcct gagacgggat gcactggccc tcgaaaaggc cggagtgaag gtgacttcgg
      301 tttttgtgga agggccggcg tgcgatacta ttctttcgga ggctctgcgc ctgaaagttg
      361 acctgattat tacgggcgca catgggcatg gcggagcaaa ctgcaaggcg tcggtcggac
      421 acatcagcga gtgcattctg ctcagatcaa aaatcccggt aatggtggtt ccggtctgac
      481 attcagatag acccttcccc gggttgcaga ccgcaaaaaa ttcagttttg cggtctgtta
      541 attttccggt tttttcattt tttgtgcttg ttttttcaaa acgccggacg catttccgaa
      601 aatgtcaaaa acccctgcaa aataagggat ttttgcgcat tttttgtcca gttttctatg
      661 gcttcattta agccgtttct gtattctgtt ttctttgaat tttgagagga aaaagaacgc
      721 ttatgtcaga tcaaaatctt gaacagcaac cgaagaaatc tgccgggtac ggcgcggacc
      781 aaatcaccgt tcttgagggg atggcggcgg tccgcaaacg tcctgccatg tacatcggcg
      841 ataccggcat ccgcggctat caccattgcg tttatgaggt cgttgacaac agtatcgacg
      901 aagcactcgc cggctactgt acgcatgttg atgtcaccat caactccgac ggttcgctca
      961 cggtcaccga cgacggtcgc ggcattccgg tggatatgca cccgaccgaa cataaaccgg
     1021 ccgtagaagt ggttctgacg gttctgcacg cgggcggcaa gttcgacaat gactcctaca
     1081 aggtttccgg cggtctgcac ggcgtcggcg tctcctgtgt aaatgctctt tcggagtggc
     1141 tcgaagtcga agtctgccgc aatggacagg tttatcatca gcgctttaaa cgcggcgcca
     1201 ccgtttcaca gctcgaagta atcgggaaaa cccagaagac cggaaccaag gttaccttca
     1261 aactcgacca cgatattttc acccacgagg gtttccagtg ggatattctg gcttcccgtt
     1321 tacgggaact ggcctttctg aatcgcggag ttcgcatccg gctggctcag gaagaaccgc
     1381 agcgcgacga ggtgttcgag tatgaaggcg gcatcagcga gttcgtacgg cacctgaaca
     1441 aaggcaaagt ggccatccat ccggatgtga tttatttcga gcgcgagcgc gacgggatta
     1501 cggcggaaat cgccatgcag tacagcgatg cctacaacga aagcatctac agctttgcga
     1561 acaacatcaa cacgatcgaa ggcggtacgc atctctccgg attccgcagc gccctcaccc
     1621 gcacggtcaa tcagtacgcg cgcaccaaca agctgatcaa agacgacaag caggccatgg
     1681 gcggcgatga tatccgcgaa ggtctgactg ccgtcatcag cgtcaaaatt cccaatccgc
     1741 agtttgaggg tcagaccaag accaaactcg gcaacgggga agtacagggc gttgtcgagg
     1801 ccatcgttaa cgaagaactc ggaacgtatt ttgaagaaaa cccgtccgtg gcccgcggcc
     1861 tgatcgaaaa ggcgatgctg gcggcacggg cgcgcgatgc cgcgcgcaaa gcccgcgacc
     1921 tgacccgccg caaaggcgcg ctcgaaagcg gcggactgcc gggcaaactg gccgactgtt
     1981 ccagccgcga tccggcgctg tgcgaactgt acatcgtaga gggcgactct gcaggcggtt
     2041 ccgccaagca gggccgcgac cgcgagtttc aggccattct gccggtgaag ggtaaagtca
     2101 ttaacgtgga aaaggcgcgt ctggacaagg tgctggcgaa cgaagaaatc cgcaccatga
     2161 tcaccgccat cggaaccggt atcggcgccg atgaattcga tctgagcaaa acgcgctaca
     2221 acaccatcgt catcatgacc gatgccgacg tggacggcgc gcacatccgc acgctgctgc
     2281 tgacgttttt ctaccggcag atgccgcagc tgatcgaagc aggttatatc tacattgccc
     2341 agccgccgct ttataaaatc aaacgcaaga agcgcgagga gtacatcgaa agcgaagccc
     2401 atctcacgcg catcctgctc gacctcggct cggaagacca gacactggtc tgcccgctcg
     2461 gcaacacgct gctggagtct gcacagcttc ccgaagtgct gcagctgctg agcgaagtag
     2521 aaaagatcgc cgggcagatg gtgcgccggg gcatggattt cgacgactac atccgccgga
     2581 tgaacagcga cggccgactg ccgtcgttcc gggttgaaat cgaccacggc aagggcgagc
     2641 cgaagatcca ttatgctttc aacgacaccg agctgcgcgc cctgcgcgag cagactgaaa
     2701 cggaaatggg caaagagctg gaaatcgcgg attcgctgac gggcggcccg ggcttccgct
     2761 ggatcgagat ccacaccgcc gaacggctgg agaagctgat tgcccagctg gatgcgaagg
     2821 gcttccacgc ccgctgtctc tcgcacctcg aaacgccgga gttttatctg acggacgaaa
     2881 aaggcgaaaa gaaagcgctg cactctctgc tggaactgct cacgacgatt cgcgagctgg
     2941 gccggcgcgg gctgtcgatt cagcggtaca aaggtctcgg ggaaatgaat cccgaacagc
     3001 tgtgggaaac cacgatggat ccggccaagc gcaaaatgac ccgcgtggtt ctggaggacg
     3061 cgttcaaggc cgaccagatg tttacgatcc tgatgggcga cgaggtcgaa ccgcgccgcg
     3121 agttcatcga aaagaacgcc ctcaatgtac agaacctcga tatttaatgc acaggaaacc
     3181 gcaccgcaat gaacacacaa caacaccagc gtattgattt gatcaatatt gaagatgaaa
     3241 tgcagcgcgc ctacatcgac tattcgatga gcgtgatcat cggccgcgcc ctgcccgatg
     3301 cgcgcgacgg cctcaagccc ggcaaccgcc gcattctgtt cgccatgaaa gagcgcggct
     3361 ggaacagcag caagccgttc gtcaaatgcg cgaaagtcgt cggggaagtg atcggtaact
     3421 accacccgca cggcgacacc gcggtctatg acaccatcgt gcgtatggcg caggacttct
     3481 ccatgcgctg catgctcatc aacggccagg gaaacttcgg ctccatcgac ggtgaccgcg
     3541 cggcggccta ccggtacacc gaatgtaaac tgctcccgct ggccgaggaa atgctggccg
     3601 atatcgacaa aaacacggtc gatatgcgcc cgaacttcga cgaaacgctg atggagccga
     3661 ccgttctgcc ggcccgcgtg cccaatctgc tcattaacgg cagcaccggg atcgccgtcg
     3721 ggatggccac caatatcccg ccgcacaatc tgagcgaggt gatcgacgga acgatccacc
     3781 tgatcgaaaa ccccgacgcc agcgtggaag atctctgcaa attcatcaaa ggcccggact
     3841 tcccgaccgc cggtattgtc cacggtctcg gcggcatcaa atcgatgtac ctgaccggac
     3901 gcggacgcat ccgtatgcgc ggcaaagcgg acatcgagga attcggcaac ggccgcgagc
     3961 ggatcatcat caccgaaatc ccctacgccg taaacaaagc cgccatgatc acccgcatgg
     4021 ccgaactggt gcgcgaaaaa aatctggagg gtatctccga tatccgcgac gaatccggca
     4081 agctcggcat ccgcgtggtc atcgagctga aaaaaggcgc gatcggcaaa gttgtgctca
     4141 acaacatcta caagcacacc cagctggcga gcaccttcgg cgccatcatg ctggcgatcg
     4201 acaataaccg cccgcgcatc atgaacctca aagaactgct gcagtgcttt atcaagcacc
     4261 gctttgaggt gatcacccgc cgcacacagt tcgacctcga taaaagccgc gcccgcgccc
     4321 acatcctgga agggctgctc atcgcgctgg ataatctcga cgaagtcgtc cggatcatcc
     4381 gcgcctccaa ggcgcgcgaa gaggcccagg cccgactgac cgaacgcttc cagttcagcg
     4441 aactgcaggt taaagccatt ctcgacatgc gcctctacca gctgacgggg ctggagcgcg
     4501 aaaagattca ggccgaatac gacgaactcc agaagctgat tgcttatctg atcgacctgc
     4561 tcgaacacga agacaagctg tacggcgtca tccgggacga cctcgccgaa atcaaagcca
     4621 aatacggcga accgcgccgc acgcagctgg aagtcgacga aggcgaaatc gacattgaag
     4681 atctgatcgc cgacgagccg tgcgccatca ccctctccaa caccggctac atcaaacgcg
     4741 tgccggtcga tacctaccgc cagcagcgcc gcggcggcaa aggcgtgatg ggcatggaca
     4801 ccaaggacga ggatttcgtc gaacacgtct tcaccgcctc gacgcacgac tatctgctgt
     4861 gctttacgca gaacggccgc atgtactggc tcaaggccta tcatgtcccg gaaggcagcc
     4921 gccaatcgcg cggacgcgcg ctggccaacg tgattgaaat gggaccggac gaaaaactgg
     4981 ccgccattct ctgcgtgcgc gagctcgacg acgagcagca ccatctgctg atggccacca
     5041 aaaacggcac catcaaaaag accgtgctgg ccgcatacaa aaacatccgc gctggcggca
     5101 tcaacgccat tggcgtggat gaaggcgacc agctcatcgg cgtacagctc accagcggaa
     5161 cggatgaaat catcctcagc ctgcgcaacg gcaaagcaat ccgcttcaac gaaacggact
     5221 gccgtccgat gggccgcacg gcgtatggcg tcaaaggcgt cactctggaa ggcgacgacc
     5281 aggttgtctc gatcgaaatc gtcaacaccg ccgccacgat gatggcgatc acggaaaacg
     5341 gctacggcaa acgcaccagc ttcgatgaat accgcctgca aagccgcggc ggcaaaggca
     5401 tcatcagcat ccagaccacg gagcgcaacg gcaaagtcgt cagcgcccac gcggtgacgg
     5461 atgagaaccg catcatgctc atttcgcaga acggtcagat gatctgcatc ggcgccagcg
     5521 acctgcgcgt gatcggacgc aacacccagg gcgtgcggct ggtcaacctg aacgaaggcg
     5581 acaagattgt ttccgccgcc gttctggatc cggaggcaga ggctgtcgaa acggtggctc
     5641 cggtttcgac ggaggtcgtt gaaggagatc tgccggaagc agacgctgag taagaccggg
     5701 tatgtcgatc gatctgagca gtctgaatct gaccccggag tggagcaaat ccactccggg
     5761 cagtagcctg ccgccgaaaa aagataaacc ggtaaaacag ccgccggctc agccgggcga
     5821 cgggattgtg cgggtcggac gggaaactaa gggccgcaag ggttccggcg tcacggtgat
     5881 cgccggaatt ccgtcgcatc ccgaagggct tgaaaaactg gcaaaacagt tcaagcagct
     5941 ttgcgggacc ggcggaacag tcaaagacgg cgtcatcgaa attcagggcg aacaccgcga
     6001 ccgactggtc gcggagctgc agaagctcgg ctataccgtc aaacgggcgg gcggatagtc
     6061 gaa
//
LOCUS       DAOPZO010000109         6043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_16208_length_6043_cov_10.699733, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000109 DAOPZO010000000
VERSION     DAOPZO010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16208_length_6043_cov_10.699733"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..475)
                     /locus_tag="PKI68_07315"
     CDS             complement(62..475)
                     /locus_tag="PKI68_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNX53537.1"
                     /translation="MVIGYLLFARGSYMAKSEFENLQVYQLAEELSDRIWNIVAAWDS
                     FAKNTVGQQLVRAVDSIGANIAEGNGRRDTRDNRRFVRIAWGSLNETQHFLRRAYKRK
                     LIKKEEAELLSPIIEALGPKLNAYHRSLTPQDEKL"
     gene            complement(479..1258)
                     /gene="proB"
                     /locus_tag="PKI68_07320"
     CDS             complement(479..1258)
                     /gene="proB"
                     /locus_tag="PKI68_07320"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNX53538.1"
                     /translation="MKETRNQLKKAKRIVVKVGSRVLVDSTGKPDHERIHALVSDIAR
                     SHDSDLEMILVSSGAIAAGIEALGMTKRPTALPDLQMAAAVGQTRLMSLYSHLFGQKH
                     IKVGQILLTNDIFQDEDRRANAQNTLLNLIKHRIVPVINENDTVATEEIKFGDNDLLA
                     ALVSILVSADALILLSTTDGLKDDDGQRVPFVEQVSDDILALDKGKNGELSTGGMKSK
                     LMSAGKAAAAGIAVIIADGQKDGIIQRILSGEDTGTFIAHG"
     gene            1423..1965
                     /gene="rsmD"
                     /locus_tag="PKI68_07325"
     CDS             1423..1965
                     /gene="rsmD"
                     /locus_tag="PKI68_07325"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNX53539.1"
                     /translation="MRINSGILRSRNFEVPKDGMRPTMEQAREAVFSSLAARVPGARV
                     LDLFAGSGSLGLEAWSRGAASVTAVEKVSGHWKILQGNFQSLQGDPDLGAWQAVQADV
                     YEYLKRGPGCFDLIFADPPYDEADLPKLLETIGGALAPDGLLVFEMRSRGAYSLPAGW
                     NLIKEKRYGGTRMLFLEQTK"
     gene            1962..2444
                     /gene="coaD"
                     /locus_tag="PKI68_07330"
     CDS             1962..2444
                     /gene="coaD"
                     /locus_tag="PKI68_07330"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNX53540.1"
                     /translation="MNKKAIYAGTFDPMTMGHLDVVERAARIFPELVVAVAAVTGKST
                     LFSQKERVELVRKAAAGLPNVTVKSFDGLLVEYARAQGAGVLIRGLRAFSDFEYEFQM
                     ALTNRRLAPDIETLFLMPKQDYSFLSSTNVKQVASLGGNVTEFVPAAVAKALARKLKK
                     "
     gene            2459..2920
                     /locus_tag="PKI68_07335"
     CDS             2459..2920
                     /locus_tag="PKI68_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNX53541.1"
                     /translation="MIIKVADIPEQGLKIRGEEPGEILELGEGDGFRAAGPVDCDLYV
                     QVVDGMLIARGTLSVPVEARCARCTQFFSTTVTDSGFLRDFPGILGAEEVDITEDVRE
                     ALILSLPHSPLCAESCKGLCIQCGKDLNDGPCECRSRKEGGPWGALNSLKL"
     gene            2992..3177
                     /gene="rpmF"
                     /locus_tag="PKI68_07340"
     CDS             2992..3177
                     /gene="rpmF"
                     /locus_tag="PKI68_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNX53542.1"
                     /translation="MALPKRKSSKSRTRRTKAENMKRSLNKAGFDAESGAPALPHRVC
                     PTTGKYKGRQVITVSDE"
     gene            3181..4197
                     /gene="plsX"
                     /locus_tag="PKI68_07345"
     CDS             3181..4197
                     /gene="plsX"
                     /locus_tag="PKI68_07345"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNX53543.1"
                     /translation="MRIAIDAMGGDFAPREIVKGAVEAARNLSGIEKLFLVGDETAIR
                     AELALYGKTPDCIEIRHASEVVGMGDSPALALRRKKDSSIMRAVELVKDGVADAIYSA
                     GSTGAAVGASMLRLRTMEKVDRPAIATVMPSIKGPFVLLDAGATPDCTPKMLAEFAIM
                     GSIYAEHILSIKNPRVGLMSIGEEDAKGNKSTKEAFQILEKTPLNFIGNVESHDMFEG
                     AVDVVVCDGFVGNVVLKTSEGAAHAIIQWIKESFSASLIRKIGAGILKASGAFAELKK
                     KTDAEAYGGAPLLGVKGICIIGHGSSSSFAVYNAIRVAGTAIEQKVNHLIEQKINEVF
                     KDSE"
     gene            4190..5194
                     /locus_tag="PKI68_07350"
     CDS             4190..5194
                     /locus_tag="PKI68_07350"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNX53544.1"
                     /translation="MSKASNSLCTVSIIGTGVYLPEKTVTNRDLEKIVDTSDEWIYSR
                     TGMRERRIARADQATSDLAAEAAKAALADAGIGADEVDLLIVATLSPDMFFPSTACFV
                     QEKIGAKNAYCYDLGAACSGSLYALETAKNQIACGAVKTALVIGAEKMSTFIDWKDRN
                     TCVLFGDGAGAMVLRSTDAKRGVMKGVFGSDGSLASLLWTPGGGSRNPMTHEMLDQNM
                     QYLKMNGRDVYKHAVTQMTHSIQDALAKNGLTSDDIKLFIPHQANARIINAISSRLGV
                     EDRTFLNVEKYANTSAAALPIAIDEAGKSGRIAKGDLMLLVAFGGGFTWGANIVEWTK
                     "
     gene            5191..>6043
                     /gene="fabD"
                     /locus_tag="PKI68_07355"
     CDS             5191..>6043
                     /gene="fabD"
                     /locus_tag="PKI68_07355"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNX53545.1"
                     /translation="MKKAILFPGQGSQSVGMGRDFYDAIPECKALFEKANEVLGYDIA
                     AICFNGPEDELKKSHNTQPAIFTVSAAALKALQLKKPGLTFDYAAGHSLGEWGALYAA
                     GVTSFEDTLRVLKARGEFIQAACIDNPGAMLAVINLDRAALEKIAGETGVTLANINSP
                     DQIVLSGTAEGIAKAEALCKAAGAKRALPLPVAGAFHSPLMQPAADRLAGFLETIQLN
                     APSMPVLSNVTGRPHENVESIRANMIAQITGSVRWVESMQWLAAQGVVEAVECGPGKV
                     LAGLMKRI"
BASE COUNT         1305 a         1638 c         1799 g         1301 t
ORIGIN      
        1 tcgtgtaagg tcatcttcga ttcccgttat tcgttattgg atatttgtta ttcggcggaa
       61 tttagagctt ttcgtcctgc ggagtcaaag accgatgata agcgttcagt ttagggccga
      121 gcgcttcgat gattggagaa agcagttcgg cctcttcttt tttgattaat ttccgcttat
      181 atgcccgccg gagaaaatgt tgagtttcgt tcaacgagcc ccaggcaatt cggacaaatc
      241 ggcgattatc tctggtgtcg cgccgcccgt ttccttcggc aatgttggcc ccgatgctgt
      301 cgacggcgcg aaccaactgc tggccaacgg tgtttttagc gaaactgtcc catgcggcga
      361 cgatattcca gatgcggtca gagagctctt cggccaactg ataaacctga agattttcaa
      421 attcactttt ggccatgtat gagcctcttg cgaataacaa atatccaata accaatatct
      481 agccgtgagc gatgaacgtt ccggtatctt ctccggagag aatccgctga atgatcccat
      541 ccttttgccc gtccgcaata atcacggcga ttcctgcggc ggcggctttg ccggcggaca
      601 tgagtttgga tttcatgccg ccggtggaga gttcgccgtt tttgccttta tcgagcgcga
      661 ggatgtcgtc ggaaacctgt tcgacgaagg gcacgcgctg gccgtcgtcg tctttgagtc
      721 cgtcggtggt ggaaagcagg atcagcgcgt cggcgctgac gagaatcgaa accagcgcgg
      781 cgagcaggtc gttgtcgccg aatttaattt cttcggtggc gacggtgtcg ttttcgttaa
      841 tgacgggaac gatccggtgt ttgatcaggt tcagaagcgt gttctgcgcg ttggcgcggc
      901 ggtcttcatc ctgaaagatg tcgttggtca gcagaatctg gccgactttg atgtgcttct
      961 gtccgaagag gtggctgtag agactcatca gccgggtctg tccgacggcg gcggccatct
     1021 ggaggtcggg cagggcggtc ggccgtttgg tcataccgag cgcttcaata ccggcggcga
     1081 ttgcgccgga agaaacgagg atcatttcca aatcgctgtc gtgcgaccgg gcgatatcgg
     1141 aaaccagcgc atgaatgcgt tcgtggtcgg gcttgccggt ggagtccacc agtacgcggc
     1201 tgccgacttt gacaacgatc cgtttggcct ttttgagttg gttgcgtgtc tctttcataa
     1261 gtaggaggcg cattatgtac aggaatcgcc cgctgaaaac acaaaaaagc gggcggattc
     1321 cggatattcg ttattggctg ttcgggcgga agccgccgga cgcgaatatc gaataacaaa
     1381 tatcgtttcg cgggctgagc gaatgcgata gcctgcgaag ctatgcgaat caacagcggt
     1441 attttaagga gccggaactt tgaggttccg aaggatggaa tgcgtccgac gatggagcag
     1501 gcgcgcgaag cggtcttttc gtcgctggcg gcgcgggtgc cgggcgcgcg ggttctcgat
     1561 ctgttcgccg ggtcgggcag tctgggtctg gaagcgtgga gccgcggcgc ggcgagtgta
     1621 acggctgtcg aaaaggtttc cggccattgg aaaatcctcc agggaaattt ccagtctctt
     1681 caaggcgatc cggatctggg cgcctggcag gcggttcagg cggacgtcta tgaatatctg
     1741 aaacgcggcc ccggctgctt cgatctgatt tttgccgatc cgccctacga cgaggccgat
     1801 ctgccgaagc tgctggaaac cattggcggc gcgctggccc cggacggttt attggtattt
     1861 gaaatgcggt cgcgcggagc ctatagtctg cccgctggtt ggaaccttat taaggagaaa
     1921 cgctacgggg gaacccggat gcttttcctg gagcagacga aatgaataag aaagcgattt
     1981 acgcgggaac attcgatccg atgacgatgg gtcacctcga tgtggtggaa cgcgcggcgc
     2041 ggatttttcc ggaactggtt gtggcggtgg cggctgtgac cggtaaaagc actctttttt
     2101 cgcagaagga gcgcgtcgag ctggtcagaa aagcggccgc cggtctgccg aacgtgaccg
     2161 tgaaatcctt cgacgggctg ctggtggaat atgcccgcgc gcagggcgcc ggcgttttaa
     2221 tccgcggatt gcgggcgttt tccgatttcg agtacgaatt tcagatggcg ctgaccaacc
     2281 gccggctggc acctgatatt gaaacgctct ttctgatgcc gaagcaggac tacagctttc
     2341 tgagttcgac aaacgtcaag caggtcgcct cgctcggcgg caatgtgacc gaatttgtgc
     2401 cggcggcggt tgctaaagcg ttggcgcgca agctgaagaa ataaacggaa cttttccaat
     2461 gatcatcaaa gtggcagata ttcccgaaca ggggttgaaa atcagaggcg aagagccggg
     2521 cgaaatcctt gagctcggcg aaggggacgg cttccgcgcc gccggtccgg tggactgcga
     2581 tctttatgtg caggtggttg acggcatgct gattgcgcgc ggaacactgt cggttccggt
     2641 cgaagcccgc tgcgcgcgat gcacgcaatt tttctcgaca accgtcaccg attccggttt
     2701 cctgcgcgac ttccccggca tcctcggagc cgaggaagtg gatataacag aggatgtgcg
     2761 cgaagcgttg atattgagtc tgccgcattc tcccctttgc gccgaaagct gcaaagggct
     2821 ctgtattcag tgcggcaaag acctcaacga cgggccgtgc gaatgccgga gcaggaaaga
     2881 gggcgggccg tggggcgcgc tcaatagttt aaaattgtaa tggaaaaacg gataggggct
     2941 ggggtaccat gccagccctt ttatacaaag tagtttaccg ggagaataga aatggccctt
     3001 ccaaaaagaa aatcatcgaa gagcagaacg agacgcacga aagctgaaaa catgaagcgt
     3061 tcgctcaata aagccggctt tgatgccgaa agcggagctc ctgctctgcc gcaccgcgta
     3121 tgcccgacca ccggcaaata caaaggccgc caggttatta ccgtctctga cgagtaagcc
     3181 atgcgtatcg caattgatgc catgggcggc gatttcgccc cccgtgagat tgttaaaggg
     3241 gccgtggaag cggcccgcaa cctttccggg attgagaagc tttttcttgt cggcgacgaa
     3301 accgccatca gggcggaact ggctctctac ggcaaaacac ctgattgcat tgaaatccgc
     3361 cacgcttcgg aagtggtcgg aatgggcgat tctcctgcgc tggcgcttcg ccgtaaaaaa
     3421 gattcctcga tcatgcgcgc cgttgagctg gtgaaggacg gcgttgccga tgcgatctat
     3481 tcggccggca gcaccggcgc ggccgtcggc gccagcatgc tccgcctgcg gaccatggag
     3541 aaagtggacc gcccggccat cgccaccgtc atgccgtcaa tcaaaggccc gttcgtcctg
     3601 ctggatgccg gcgcgacacc tgactgtacg ccgaaaatgc tggcggaatt cgccatcatg
     3661 ggcagcatct acgccgagca catcctttcg ataaagaacc cgcgcgtcgg tctgatgagc
     3721 atcggcgagg aagacgccaa gggaaataaa tccaccaaag aggcgttcca gattctggaa
     3781 aaaacgccgc ttaacttcat cggtaacgtg gaaagccacg atatgttcga gggagcagtg
     3841 gacgtcgtgg tctgcgacgg ctttgtcggc aatgtggttt tgaaaaccag cgaaggcgcc
     3901 gcacacgcaa tcattcagtg gatcaaagag tctttttcgg cttcgctgat ccgcaagatc
     3961 ggcgccggta ttctgaaagc ttcgggcgcg tttgccgagc tgaagaaaaa aaccgacgcg
     4021 gaagcctacg gcggcgctcc gctgctgggc gttaaaggaa tctgcattat cgggcacggt
     4081 tcttcttctt cgtttgccgt ttataacgcc attcgcgtcg cgggcaccgc gatcgaacag
     4141 aaggtaaacc acctgatcga acagaagatc aatgaagttt tcaaagacag tgagtaaagc
     4201 atccaacagt ttatgcacgg tttcgattat cggaaccgga gtttaccttc cggaaaaaac
     4261 ggtcaccaac cgtgatctgg aaaaaatcgt cgataccagc gacgagtgga tctattcacg
     4321 taccggcatg cgcgagcggc gcattgcccg cgccgatcag gccacatccg atctggccgc
     4381 tgaagccgcc aaagcggcgc tcgccgacgc cggtatcggc gcggacgaag tggatctgct
     4441 gattgtcgcc acgctttccc ccgatatgtt tttccccagc accgcctgct tcgtgcagga
     4501 gaaaatcggc gcgaaaaacg cctactgcta cgatctcggc gcggcctgtt ccggttcgct
     4561 ctacgcgctc gaaaccgcca aaaaccagat cgcctgcggt gcggtcaaga cagcgctggt
     4621 gatcggcgcg gaaaaaatga gcacgtttat cgactggaaa gaccgcaaca cctgcgtgct
     4681 cttcggtgac ggcgcgggcg cgatggtgct gcgctccacg gatgcaaaac gcggcgtcat
     4741 gaaaggcgtt ttcggttcgg acggttcgct ggcttcgctg ctctggacgc ccggcggcgg
     4801 ctcgcgcaat ccgatgacgc acgagatgct cgatcagaac atgcagtatc tcaaaatgaa
     4861 cggccgcgat gtttacaaac acgccgtaac ccagatgacg cactcgatac aggatgcgct
     4921 cgctaaaaac ggtctcacct ccgacgacat taaacttttt attccgcatc aggccaatgc
     4981 ccgcatcatc aacgccatca gctcgcgcct cggcgtcgaa gaccggacct tcctgaatgt
     5041 cgaaaaatac gccaacacct cggcggccgc gctgccgatc gccattgacg aggcgggcaa
     5101 atcaggccgg atcgctaaag gcgatctcat gctgctggtt gctttcggcg gcgggtttac
     5161 ctggggtgcc aatattgtgg agtggacaaa atgaagaaag ccatcctttt ccccggacag
     5221 ggttctcagt cggtcggcat gggccgcgat ttctatgacg cgattccgga gtgcaaagcg
     5281 ctctttgaaa aagccaacga agtactcggc tacgacatcg ccgcgatctg ctttaacggt
     5341 ccggaagacg aactgaaaaa gtcgcacaac acgcagcccg cgattttcac cgtcagcgct
     5401 gctgcgctca aagctctcca gctcaaaaaa ccgggcctta cttttgatta tgccgccgga
     5461 cacagtctcg gcgagtgggg tgcgctctac gccgccggtg tgacctcctt tgaagatacg
     5521 ctgcgtgttc ttaaagcccg cggcgaattc attcaggccg cctgcatcga caatcccggc
     5581 gcaatgctgg ccgtcatcaa cctcgaccgc gcggcgctcg aaaagatcgc cggcgaaacc
     5641 ggcgtgacgc tggcgaatat caattcgccc gaccagattg ttctttccgg aaccgccgaa
     5701 ggcatcgcga aagccgaagc gctctgcaaa gccgccggtg ccaagcgcgc tctgccgctg
     5761 ccggtcgccg gagcgttcca ctctccgctg atgcagcccg ccgccgaccg gctcgccggt
     5821 tttcttgaaa ccattcagct gaatgcgccc tccatgcccg ttctttcgaa tgtcaccggg
     5881 cggccgcatg aaaatgttga atcaatccgc gcgaatatga tcgctcagat caccggttca
     5941 gtccgctggg ttgaatccat gcagtggctt gccgcacaag gtgttgttga agcggttgaa
     6001 tgcggtccgg gtaaagtttt ggcggggctg atgaagcgga ttg
//
LOCUS       DAOPZO010000110         6035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_16237_length_6035_cov_8.634615, whole genome shotgun sequence.
ACCESSION   DAOPZO010000110 DAOPZO010000000
VERSION     DAOPZO010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16237_length_6035_cov_8.634615"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..82)
                     /locus_tag="PKI68_07360"
     tRNA            complement(8..82)
                     /locus_tag="PKI68_07360"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48..50),aa:Asn,seq:gtt)
     gene            complement(167..772)
                     /gene="recR"
                     /locus_tag="PKI68_07365"
     CDS             complement(167..772)
                     /gene="recR"
                     /locus_tag="PKI68_07365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNX53546.1"
                     /translation="MKHFLPLDNLVAALEKLPGIGKRSAERMAFALVQNRDGLARRLA
                     DALLAADEQVGTCSRCGGITVTKEDPCALCTEGKRANGLLCVVESPGDIMKIETSGGF
                     NGRYHVLGGHLSPALGRGVADLRVNELLARLQPENIQEVIVALGTDVESEATAGYLAE
                     IIASRGVKASRLAFGMPSGSAIEYTDSTTLARAIQGRQSLG"
     gene            complement(775..1065)
                     /locus_tag="PKI68_07370"
     CDS             complement(775..1065)
                     /locus_tag="PKI68_07370"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNX53547.1"
                     /translation="MDMMKMMKQVAEMKKIQKQLASKKVEFSSAAGQVTVKMTCDMKP
                     HSITIAPELIQSGNVKKIEAALLDAFKGVLNAAQMEAAKDMKSLTAGLNLPF"
     gene            complement(1081..2670)
                     /gene="dnaX"
                     /locus_tag="PKI68_07375"
     CDS             complement(1081..2670)
                     /gene="dnaX"
                     /locus_tag="PKI68_07375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNX53548.1"
                     /translation="MGYEVLARKWRPQVFADVVGQNHVTQTLHNAIKNDRLAHAYLFV
                     GPRGTGKTTTARILAKALNCKNRQGSEPCNACDSCKEIMKGSSLDVIEIDGASNRGIG
                     DVQELRDNVRYAPVRGPFKIYIIDEVHMLTREAFNAILKTLEEPPAHVKFFFATTEPQ
                     KVLTTILSRCQRFDLRRITVADITAQLQKIAGAEGIKISADALLAIARGAEGGLRDAE
                     SALDQLVSFRGRTIEEADVLSVFGLISRAQLEELTDAILDGNIPAIIGTIAGIDASGK
                     DMQRVVLELLDQFRNLLIVMHAGKNAADFDLPETQIDTLKKQAAKSSDGRLLRIIELL
                     IDADSRMRYALSKRTMLETALIRASRAATVATIDELMKQVAALKTLPAAPAAVQEAPA
                     GYDVKKKPTDDLEPLKAAWPTLIEHISKGAPQAKHYLTDTVPLSASETHVTIGIDPEF
                     AGERANLENPRTLLLIQKTLSAFLKRPVSAGFSVLAGKTKPLPTDAPVQGIQDKQKYY
                     SNPSVRSVLETFSGEIIEIRE"
     gene            2792..3154
                     /locus_tag="PKI68_07380"
     CDS             2792..3154
                     /locus_tag="PKI68_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HNX53549.1"
                     /translation="MRISFILLLVVLTSAGLVRGAATANELTHTEDVVQDMSSKLNRG
                     VANILTGWGEIPRQMIISGQDKGWWAVLPVGIPAGAIMTVVRTGVGVFETGLFFIPIN
                     DSYEPIIDPAFVWQKSKS"
     gene            3151..3906
                     /gene="surE"
                     /locus_tag="PKI68_07385"
     CDS             3151..3906
                     /gene="surE"
                     /locus_tag="PKI68_07385"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNX53550.1"
                     /translation="MRILICNDDGIHAPGISVLHDAVKEFGERCVIAPDVERSAAGHA
                     ITLADPIKSTPVDKNGEFFGHAIGGTPADCIKLAVCAILKDTPPDLVLSGVNLGSNTG
                     ISVIYSGTVSAATEGVVLGIPGVAFSLCSYTDPEWEMAGRVAREITQKIIETPMPRGV
                     LLNVNMPNLPYEQLKGIKITRMGRSRFIEKFHKRLDPRGRTYYWLDGELEVQDDGDDI
                     DIHAVRDGYVSITPIHLDLTAYEHLKTFEFLKA"
     gene            complement(4030..5940)
                     /locus_tag="PKI68_07390"
     CDS             complement(4030..5940)
                     /locus_tag="PKI68_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX53551.1"
                     /translation="MRPIHYLLFCALSLCLTHPCRGADIPPNSVLLEAEAFQNTGGWV
                     VDSQFMDLMGSPFLMAHGLGEPVTDAATTLELPEAGTYRVWVRTRDWVAPWNVAGAPG
                     KFQLVINGQPLPAVFGTEGANWHWQDGGTVTLAKANQIALHDLTGFNGRCDAIFLCRD
                     QKFTPPDGGAELAAMRQKMLGWTTTPADGGQYDIVVAGGGVAGICAAITAARDGLKVA
                     LVQDRPVLGGNSSSEIRVWPEGETNFQPFPRIGDVGKELLPATNSLGRGNAKNGDVYE
                     DAKKLALVRAETNITLFLEQRINQAQCENGKIQSVVAQDIRSGRRQQLRARWFVDATG
                     DGVLGTLAGADSDMIIGNNMGSSNLWNVDAIEKNEMQLLCERTGQDPLSMKFTKSDKP
                     APFPRCPWAVDLAQKPFPGRKEAKEQGASKKPPLSKLGGWAWESGFGLDPIQDMERIR
                     DQNLRAMFGAWDALKNVEGLYPNYRLKWSAYIAGKRESRRLLGDVVLTAEDFRNNTVF
                     PDPAFPCSWRIDLHSPDRAYVTENPQDAFISNSTHGGNYKYNKPYWAPYRTLYSRNVA
                     NLFMAGRDISVNHEALGPVRVMRTTGMMGEVVGMAAGLCKKYDCTPREVYEKHLEELQ
                     QKMRTGSGKPKP"
BASE COUNT         1122 a         1692 c         1817 g         1404 t
ORIGIN      
        1 tttaaagtgg ctccggcggc tgggctcgaa ccagcaacca agtgattaac agtcacctac
       61 tctaccattg agctacgccg gaattaagag ccgcctaagg tatagaaatc gtctgctaag
      121 tcaagacact gaaacgcaaa atcaccgggt ttcgaagtcc gggtgttcaa ccgagcgatt
      181 gacggccctg aatggcgcgg gcgagggtgg tcgaatccgt atattcaatg gcgcttccgg
      241 acggcatgcc gaaggcgagc cgtgaggcct ttacgccgcg gctggcgatg atttccgcca
      301 gatagccggc ggttgcttcg ctttcgacgt ccgtgccgag cgctacaatc acttcctgga
      361 tattttcggg ctggagcctc gccagcagtt cattcacccg cagatcggcc acacctctgc
      421 cgagtgcggg tgaaaggtgg ccgcccagta cgtggtaacg cccgttgaag ccgccggatg
      481 tttcaatttt catgatgtcg ccgggacttt ccacgacgca gagcagcccg ttggcgcgct
      541 tcccttcggt gcagagcgcg cagggatctt ctttggtgac ggtgatgcct ccgcaccggg
      601 aacaggtgcc aacctgttca tcggcggcga gcagggcgtc cgccagcctt ctggccagcc
      661 cgtcgcggtt ctgcaccagc gcaaacgcca tccgctccgc ggagcgtttc ccgatgcccg
      721 gaagtttttc cagcgccgca accaggttgt ccaatggaag gaagtgtttc attgtcagaa
      781 cggcagattc aggccggcgg tcagactctt catgtcttta gccgcttcca tctgtgcggc
      841 attcagtaca cccttgaacg catccagcag ggctgcttcg atttttttta cattgccgga
      901 ctgaatgagt tcgggagcaa tagtgatgct gtgcggcttc atgtcgcagg tcattttaac
      961 agtcacctgg ccggcagcgc tggagaattc aactttcttt gaagccagct gcttctggat
     1021 ttttttcatt tccgcaacct gcttcatcat tttcatcata tccataggtc agatcctttc
     1081 ttactctctg atttcaatta tttcgccgct gaaggtctca aggacgctcc ggacggacgg
     1141 attgctgtaa tatttctgtt tatcctgaat gccctgcacc ggcgcatcgg tcggcaacgg
     1201 tttggttttt ccggccagta cgctgaagcc ggccgagacc ggacgtttca gaaaggcgga
     1261 cagggttttc tggatcagca aaagggttcg cggattttcg aggttggcgc gttcgccggc
     1321 gaattcgggg tcgataccga tggtgacgtg tgtttcagag gcgctgagcg gaacggtgtc
     1381 ggtcagatag tgtttagcct gcggcgcgcc tttcgagatg tgttcaatca gcgttggcca
     1441 cgccgctttg agcggctcta aatcatccgt gggctttttt tttacgtcgt atcccgccgg
     1501 cgcttcctga accgcagccg gtgcagccgg cagcgttttg agggcggcga cctgtttcat
     1561 cagctcgtcg atcgtggcga cggtggcggc gcgcgaggcg cggatgagcg cggtttcgag
     1621 catggttcgc ttggaaaggg cgtagcgcat ccggctgtcg gcatcgatca gcagttcgat
     1681 gattcgcagc aggcggccgt cggaactctt ggcggcctgt tttttgagcg tatcaatctg
     1741 cgtttccggc agatcaaaat cggcggcatt tttgccggca tgcatgacga tcagcagatt
     1801 gcggaactgg tcgagcagtt cgagcacgac gcgctgcata tctttgccgc tggcatcaat
     1861 gccggcgatg gttccgatga tggcgggaat gttgccgtcg agaatggcgt cggtcagctc
     1921 ttcaagctgg gcgcggctga tcagcccgaa aacggagaga acgtccgctt cctcgatggt
     1981 cctgccgcgg aacgaaacga gctggtcgag cgcgctttcg gcgtcgcgca gtccgccttc
     2041 cgcgccgcgg gcgatggcga gcagggcgtc ggcgctgatt tttattcctt ccgccccggc
     2101 gatcttctgc agctgcgcgg taatgtcagc tactgtgatg cggcgcaggt caaagcgctg
     2161 gcagcgcgaa agaatcgtag tgagcacttt ctgcggttcg gtggtggcaa agaaaaactt
     2221 cacgtgggcc ggcggttctt cgagcgtttt gaggatcgcg ttgaaggctt cgcgggtgag
     2281 catgtgaact tcgtcgatga tatagatttt gaacgggccg cgtaccggag catagcggac
     2341 gttgtcgcgc agttcctgca cgtcgccgat accgcggttg gaagcgccgt cgatttcgat
     2401 gacgtcgagg ctgctgcctt tcatgatttc tttgcaggaa tcgcaggcat tacacggttc
     2461 ggatccctga cggttcttgc agttcaatgc tttggccaga atgcgtgcag tggtggtttt
     2521 gccggtgccg cgcgggccga caaacaggta ggcgtgcgcc agccggtcgt ttttaattgc
     2581 gttgtgcaat gtctgcgtga cgtggttctg acccaccaca tccgcaaaaa cctgcggccg
     2641 ccatttccgt gctagaactt catatcccat gagccgtaga aggtaccgac ccctctgccg
     2701 aatggcaaga tgcgaaggga gggattgata aaaatcttgt cgccttacgc gggttcaact
     2761 agcctctgcc tgataatcag gagggcttgc tatgcgtatt tcatttattc tgctgcttgt
     2821 ggtgctgact tctgccgggc tggttcgcgg cgcggcgacg gctaacgagc tcactcatac
     2881 tgaagatgtc gtgcaggata tgtcttctaa attaaaccgc ggtgtcgcaa acatcctgac
     2941 cggctggggc gaaattccgc gccagatgat catatccggg caagacaaag gctggtgggc
     3001 ggttctgccg gtcggtattc ctgccggtgc tattatgacg gtcgtccgca ccggagtcgg
     3061 cgtatttgaa accggccttt tctttattcc gattaatgat tcgtatgaac cgatcattga
     3121 tccggctttt gtctggcaga aaagtaaatc atgaggattt taatttgtaa cgatgacggc
     3181 attcacgcgc ccggtatttc cgtgctgcat gatgccgtca aagaattcgg cgagcgttgt
     3241 gtcattgcgc cggatgtgga acgcagcgcc gcaggacacg ccattacgct ggccgacccg
     3301 atcaagagca cgccggttga taaaaacggc gagttcttcg gccacgccat cggcggaacg
     3361 ccggcggact gtatcaaact ggctgtctgc gcgattctga aagacacgcc gcccgatctg
     3421 gtgctcagcg gcgtcaatct cggttctaac accggcatca gcgttatcta ttccggaacg
     3481 gtttccgcgg cgaccgaagg cgttgtgctg ggtattcccg gcgtcgcctt ttcgctttgc
     3541 tcctatacgg atcccgaatg ggaaatggcc gggcgcgtgg ccagagaaat tacgcagaaa
     3601 atcattgaaa cgccgatgcc gcgcggcgtg ctgctcaatg tcaacatgcc caatcttccg
     3661 tatgagcagc tcaaaggaat taaaatcacc cgtatgggcc gttcgcggtt tattgaaaag
     3721 tttcataaac ggctcgatcc tcgcggacgc acctactact ggctcgatgg cgaacttgaa
     3781 gtgcaggatg acggcgatga cattgatatt cacgccgtgc gcgacggcta tgtttccatc
     3841 actccgatcc atctcgacct gacggcctac gaacacctga agacgtttga gtttttgaag
     3901 gcttgagccg cgcggccggg agaagcaaaa aaggcggaca ccgcaacggt gtccgccttt
     3961 tttgtgccct gcggggcatc cggcagctcc gggccgctcc acgatgagtg ggcgcggccg
     4021 ggccaccgtt tacggttttg gtttgccgct gccggtccgc attttctgtt gcagctcctc
     4081 cagatgcttt tcgtagacct cgcgcggcgt gcagtcgtat ttcttgcaca gcccggcggc
     4141 catgccgacg acctcgccca tcatgccggt ggtgcgcatg acgcggacgg gacccagcgc
     4201 ctcatgattc acgctgatgt cgcgtccggc cataaacagg tttgcgacgt tccggctgta
     4261 gagggtccgg taaggggccc agtacggctt gttgtatttg tagttgccgc cgtgggtcga
     4321 gttcgaaatg aaggcgtcct gagggttctc ggttacgtat gcacgatccg gggagtgcag
     4381 gtcgatgcgc cagctgcagg ggaaggccgg gtcggggaat acggtgttgt tgcggaaatc
     4441 ctctgccgtg aggacgacat cgccgagcag ccggcgggat tcgcgctttc cggcgatgta
     4501 ggccgaccat ttcaggcgat agttcgggta gagcccttcg acatttttga gtgcatccca
     4561 ggctccgaac atcgcgcgca agttctggtc gcggatgcgc tccatatcct gaatcggatc
     4621 cagaccaaac ccgctttccc aggcccatcc gccaagcttg ctgagcggcg gtttcttgct
     4681 cgccccttgt tctttggctt ctttgcggcc gggaaacggt ttttgagcca gatcgacggc
     4741 ccacgggcag cgcggaaacg gagccggttt gtcggacttg gtgaatttca tcgacagcgg
     4801 atcttgaccg gtgcgctcgc acagcagctg catttcgttc ttttctatcg catccacatt
     4861 ccagagattg ctggagccca tgttgtttcc gatgatcata tcgctgtccg ccccggccag
     4921 tgtgcccaga acgccgtcgc ccgtagcatc gacaaaccag cgcgcacgca gctgctggcg
     4981 ccgtccgctg cggatatcct gcgcaaccac cgactggatt ttgccgtttt cacactgcgc
     5041 ctgattgatg cgctgttcca gaaagagggt gatgttggtt tccgcccgca ccagagcgag
     5101 tttttttgca tcttcgtaga cgtcgccgtt tttggcgtta ccgcgcccca gcgaattggt
     5161 ggcaggaagc agctctttgc cgacatcgcc gatgcggggg aacggctgga aattggtttc
     5221 gccttccggc catacccgta tttccgagct ggagttgccg ccaagaaccg ggcggtcttg
     5281 taccaaagcc accttgagcc cgtcccgtgc ggcggtaatc gctgcacaga tgccggccac
     5341 tccgccgccg gcaactacga tatcgtactg gccgccatcc gcgggagtgg tggtccagcc
     5401 cagcatcttt tgacgcatcg cggccagctc ggcgccgccg tccggaggcg taaacttctg
     5461 gtcacggcac agaaagatgg catcgcagcg gccgttgaac ccggtcagat catgcagggc
     5521 gatctggttg gctttggcca gcgtgacggt cccgccgtcc tgccaatgcc agttcgcccc
     5581 ctcggtaccg aagacggcgg gcaggggctg gccgttgatg accagttgga attttcccgg
     5641 cgcacccgcc acattccacg gggcgaccca gtcgcgggtc cgtacccaga cgcggtaggt
     5701 tccggcctcc ggcagttcca gagtggtggc ggcatccgtc accggttcgc ccagtccgtg
     5761 cgccatcaga aacggggagc ccatcagatc catgaactgc gagtcgacga cccagccgcc
     5821 tgtattctga aaggcttcgg cttcgaggag taccgagttg ggcgggatgt cagcaccccg
     5881 gcagggatgc gtcaggcata agctgagtgc gcagaacaga aggtagtgta tcggcctcat
     5941 cggggaactc tccggggtta ggttgtattg tatgtctgta tctcaaatta caaataaatg
     6001 ttcatttata cgctatgcgc tcagaattgt ccatt
//
LOCUS       DAOPZO010000111         6013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_16319_length_6013_cov_9.426821, whole genome shotgun sequence.
ACCESSION   DAOPZO010000111 DAOPZO010000000
VERSION     DAOPZO010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16319_length_6013_cov_9.426821"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..976
                     /gene="folP"
                     /locus_tag="PKI68_07395"
     CDS             86..976
                     /gene="folP"
                     /locus_tag="PKI68_07395"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNX53552.1"
                     /translation="MFSPMFGKTQIIWRCRGREVVCGQKTLIMGILNVTPDSFSDGGK
                     FVDPEQAVARGLQMVAEGADIIDIGGESTRPGSKPVPAKEEIRRTIPIIAELKKQSRG
                     LISIDTRNAETARAAIAAGADIINDISALADPQMVNVAAETGAGLVLMHMQGTPQTMQ
                     NDPRYGDVVSEVGRFLEERAAFAIERGVAPEQIALDPGIGFGKTDEHNLALLRGIPAL
                     AAAGRPVLIGASRKGFIGRLTGCEPADRLAGSLAAAVFSMLRGAQILRVHDVKESCDA
                     ARLVDTLRAQGTHDELAAQY"
     gene            954..1802
                     /gene="cdaA"
                     /locus_tag="PKI68_07400"
     CDS             954..1802
                     /gene="cdaA"
                     /locus_tag="PKI68_07400"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNX53553.1"
                     /translation="MNWLHSIEYPGGTGTVEILILAVVFYFLVKFFRGTRGAAILTGL
                     SILFAFLMVLTKFTNLQVLNWLLQGLVVYLALALVVIFQPEIRRALARLGRQGNFMAS
                     KARRALTDPIADAVLMMASRKIGALIAIERESETRGIQDTGTVLNSEVSAELLTSIFY
                     PGTPLHDGGVIIGDERIIAAGCVFPLTQTDTLTSDLGTRHRAAIGLTEETDALVIVVS
                     EETGIISVAHNGRLKRGFDGSRLRRVLTSFVGHEGGGLQRRFTRRRTAEANEQRNFIF
                     GEGEND"
     gene            1810..2769
                     /locus_tag="PKI68_07405"
     CDS             1810..2769
                     /locus_tag="PKI68_07405"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNX53554.1"
                     /translation="MKISMPPFSLKPVWRSLTRHWIMKLFCIILAFAVWQSVRENISF
                     EVVVADVPVTVTAGEGRAVSDKSTDTVNITFRGSRDDIRFISSDQIEMKIDVSKHSNR
                     LRQTVKFSPRYVKAPSRAHAVRFDPPEVTVTIDREVERVLPVKAVLEGELPPGVQMEQ
                     AVCTPASVKVRGAERLLSELEQVRTVPVSLDGRYNSFKTHVAVASAGQPWTVFPERVT
                     AEISLVQGVAIRRIENSYVRPLMVSDDTRTVRIRPEKVAVTLRGSPQRTEELNARDVY
                     TYIDCTELREAADYEVPVRVDVPAGLQLEKIEPAAVKVTVKKM"
     gene            2777..3646
                     /locus_tag="PKI68_07410"
     CDS             2777..3646
                     /locus_tag="PKI68_07410"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX53555.1"
                     /translation="MRYLFFILISGLPASFVMAQGVPQGPSPLNIGVYEPETNTAVKA
                     GADAAQGSPRGPSPLIIVTDEKQTNITVQTGIEAPEGSQDLMLKVSGGTAETAKPAVR
                     SESSGTTNEVKSARISFLMNTGVQYADAGDYPEAERAYLRARDADPDNPNILFSLSSL
                     YIQMGRYKDAIDILNGMAVKFPENALVHNNLAWAYSTGGAMKNGKLAVRHAIEAIMSD
                     PIEASMWHTLAEAFYVSGQYDKALSACEYAVDLLKMQEASAEGIAEFEKQRTKILRAA
                     EATKRMMNLDDKK"
     gene            complement(3647..4597)
                     /locus_tag="PKI68_07415"
     CDS             complement(3647..4597)
                     /locus_tag="PKI68_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53556.1"
                     /translation="MLKTSMERVYYRFFVLLTVTAACTAFSFADDLDASLEAQKKKAQ
                     RHVYSDVALLHNQNLFVPRETSAEEKNLDKKLEAMEAEQDAQASARQLAARGSGAATL
                     ARPAENKNWLTPALLDNDTESSLTNSAAEDSWLVRELDRQKELKAKEAAATKDKELVD
                     RLLREKTSPKSNSPELDRLKKYQPAPPQLFGSKEKDPDAPLYMTPQSGIPDPTEAVRL
                     TSRKDTPVTPPLFSPQSSRVSSFPDKDPLRSTRSPSLTPNLGASARPSSIRSTLSPDW
                     SSPAPPVLTPMEMIRKSSPINKPDPFADDRMPHVKTSIWQ"
     gene            4734..>6013
                     /locus_tag="PKI68_07420"
     CDS             4734..>6013
                     /locus_tag="PKI68_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNX53557.1"
                     /translation="MPAVVSEFRLDQNRLWLAAENEPLPQLLGHFAAAGIDVQIDPDA
                     QKTVTGSCRAADVEEVLDKLLTPYSYLLDWRREPGPLGDLIRLTGIRVFRQGHASAVQ
                     PLRAARRIETSLDGRFRYLAREILIGFKPGSSIENLRALLARTGGTVIAANAELGIYR
                     ILLPEGANVLDLAGQLGNESSLALAEPNYVYDLPGLLPGEKNTSAAPAQWTAPDKSGK
                     IAVSVLDSGLLADSSLDRAVISAFDATSPGSPLTADAVGHGTLMAQLASGLLDPYNSP
                     VGEGVPVVAVKAFDDKGLADSFTLMSAMTYAVKNSSGPVSLSWGSETPSKFIESAVKY
                     AISQDRPVFAAVGNENTGRPMYPAAYPGVVGVAASNGDRLADYSNRGDFVDIIAPGSA
                     GGSQGTSVATAYVAHIAALYMKNHPGVSAADTVSA"
BASE COUNT         1225 a         1727 c         1788 g         1273 t
ORIGIN      
        1 aaactaaaga ctctgttccg gaatacaagc gcagagccgc atgatgcggc gggcattttg
       61 tttttcaggg tcgggaaaac agagtatgtt ttctccgatg ttcggaaaaa cacagatcat
      121 ttggcggtgc cgcggacggg aagttgtctg cgggcagaaa acgctgatta tgggcatcct
      181 gaacgtgacg ccggattcgt tttcggacgg cggaaagttc gtcgatcctg aacaggcggt
      241 ggcgcgcggc ctccaaatgg ttgcggaagg cgcggatatc atcgatatcg gcggcgaatc
      301 gacccggccg ggttcgaagc cggttccggc gaaagaggag attcgtcgaa cgatcccgat
      361 tatcgcagag cttaaaaagc agtctcgcgg tctgatttcc attgatacac gcaacgcgga
      421 aaccgcccgc gcggcaatcg ccgccggagc agatattatc aacgacattt ccgcgctggc
      481 cgatccgcag atggtgaatg ttgccgccga aaccggcgcg gggctggtgc tgatgcatat
      541 gcagggcacg ccgcaaacga tgcagaacga tccgcgctac ggcgacgtgg tttcggaggt
      601 cggaaggttt ttagaggaac gggcggcgtt cgctatagaa cggggcgttg caccggaaca
      661 gattgcgctc gatccgggta tcggattcgg caaaaccgat gaacataatc tggcgctttt
      721 acgcggtatt ccggcgctgg cggcggccgg ccggccggtg ctgatcggcg cgagccgcaa
      781 agggtttatc ggccggctga ccggctgcga accggctgac cggctggcgg gttcgctggc
      841 ggcggcggtc ttttccatgc tgcgcggcgc gcagattttg cgggtgcacg atgtaaagga
      901 atcttgcgac gccgcgcggc ttgtggatac actgcgcgct caaggaacgc atgatgaact
      961 ggctgcacag tattgagtat cccggaggaa ccggaacggt cgagattctg attctcgccg
     1021 tcgtttttta tttcctggtg aagtttttcc gcggtacgcg cggcgcggct attctgacgg
     1081 ggctttccat cctttttgcg ttcctgatgg ttctcaccaa gttcaccaat ttgcaggttc
     1141 ttaactggct tctgcagggg ctggtggttt atctggcact cgcgctggtg gtgattttcc
     1201 agccggaaat ccgccgcgcg ctggcccggc tcggacggca gggcaatttt atggcgagca
     1261 aggcccgccg cgcgttgacg gatccgattg ccgacgcggt gctgatgatg gcttcgcgaa
     1321 aaatcggcgc attgattgca attgaacgcg agtcggagac ccgcgggatt caggataccg
     1381 gcactgtgct gaacagcgag gtgagcgcgg aactgctgac gtcgattttt taccccggta
     1441 caccgctgca cgacggcgga gtgattatcg gcgacgagcg cattattgcg gccggctgcg
     1501 tttttccgct gacgcagacg gatacgctga ccagcgatct cggtacgcgc caccgcgcgg
     1561 ccatcggcct gacggaggag acggatgcac tggtaattgt tgtttcggaa gaaaccggca
     1621 ttatctcggt cgcacataac ggccgcctca agcgcggatt tgacggatcg cgcctgcgcc
     1681 gtgttctgac ttcgttcgtt ggtcatgaag gcggcggcct gcagcggcgg ttcacccgcc
     1741 gccgtaccgc cgaagcgaat gaacagcgga attttatttt cggggagggt gaaaatgatt
     1801 aagcggggca tgaaaatatc tatgccgccg ttcagtctga aacctgtctg gcggtcactt
     1861 acccgtcact ggataatgaa actgttctgc atcatactgg ctttcgcggt gtggcagtcc
     1921 gtccgtgaaa acatcagttt tgaagtggtc gtggcggatg ttccggttac cgtaaccgca
     1981 ggggagggcc gggcggtttc cgacaagtcc accgatacgg tcaatataac gtttcgcggt
     2041 tcgcgtgatg atatccgttt catcagcagc gatcagatcg aaatgaaaat tgatgtttca
     2101 aaacattcca accggctgcg ccagacggtt aagttttcgc cgcgctatgt caaagcgccg
     2161 tcgcgggctc atgcggtgcg gttcgatccg ccggaagtga ccgtgacaat cgaccgtgaa
     2221 gtggagagag ttcttccggt gaaggccgtg ctggaaggtg aacttccgcc aggcgttcag
     2281 atggagcagg cggtctgtac gcctgcttct gtaaaggtgc gcggcgctga gcggcttctg
     2341 agtgaactgg aacaggtgcg aaccgtgccg gtcagtctcg acggtcgcta caactcgttt
     2401 aaaacccatg tggcggtcgc ctcggccggc cagccgtgga cggttttccc ggagcgcgtt
     2461 acggccgaga tttcgctggt tcaaggcgtg gcaatccggc ggatcgaaaa cagctatgtg
     2521 cgtccgctga tggtttccga cgatacgcgg acggttagaa tccggccgga aaaagttgct
     2581 gtaacgctgc gcgggagtcc gcagcggact gaagaattga atgcgcgtga tgtttacacc
     2641 tacatcgact gcacagaact gagagaagcc gcagactatg aagttcccgt gcgcgttgat
     2701 gtgccggccg gtctgcagct cgagaaaatt gaacccgcag cagtcaaagt gaccgtcaaa
     2761 aaaatgtgag cagcttatgc gatatctatt cttcatttta atcagcgggc ttccggcctc
     2821 ttttgtaatg gcgcaaggag ttcctcaggg cccgtcaccg ctgaatatcg gcgtttatga
     2881 gccggaaaca aatacggccg tgaaggccgg ggccgatgcg gcccaaggga gtccacgcgg
     2941 accgtcgccg ctgatcatcg tgacggatga aaaacaaacc aacattacag tgcagaccgg
     3001 aattgaggcg ccggagggtt cgcaggatct tatgctgaag gtgtccggcg gaactgcaga
     3061 gacggcgaaa cctgccgtgc ggagcgagtc ctccggaacg accaatgagg taaaatcggc
     3121 ccggatttct tttctgatga ataccggcgt tcagtatgcc gacgcgggcg actatccgga
     3181 agccgaacgg gcttacctgc gtgcgcggga cgccgatccg gacaatccga acattctgtt
     3241 cagcctgagt tcgctctaca tccagatggg tcggtataaa gacgcgatag atatattgaa
     3301 cggcatggcc gtaaagtttc cggagaatgc tctggttcac aacaacctgg cctgggctta
     3361 ttccaccggc ggtgcgatga aaaacggaaa actggcggtg cggcacgcga tcgaggcgat
     3421 catgtccgac ccgattgagg cctcgatgtg gcatactctg gccgaagctt tttatgtttc
     3481 cgggcagtat gacaaagcgc tgagtgcctg cgaatatgcc gttgatctgc tcaaaatgca
     3541 ggaggcttct gccgaaggga ttgccgagtt cgaaaaacag cgcacgaaga ttctgcgggc
     3601 cgccgaagcg acaaaaagga tgatgaacct ggacgataag aaatagttac tgccagatgc
     3661 tggtcttgac gtgcggcatg cgatcatccg caaacggatc cggtttattg atcggcgacg
     3721 atttcctgat catttccatc ggagtcagta caggcggcgc cggcgaactc cagtcgggcg
     3781 acagcgtaga gcggatagac gatggccggg cggatgcgcc gagattcggg gtgagcgacg
     3841 ggcttctggt ggaccggagc ggatctttgt ccggaaagga agaaactctg gaagattgcg
     3901 gagagaataa aggcggtgta accggcgtat ctttccttga tgtcaggcga accgcttctg
     3961 tcgggtcggg gattccgctt tgcggagtca tatataaagg agcatcggga tccttctctt
     4021 tactgccgaa aagctgcggc ggagcaggct ggtatttttt cagccgatcc agctccggcg
     4081 aattgctctt cggcgaggtt ttttcgcgga gaagtctgtc aaccagctct ttatctttcg
     4141 ttgccgcggc ctccttcgcc ttcagctcct tctggcgatc cagctcccgg acaagccacg
     4201 agtcttcggc cgcgctgttg gtcagcgaag actcggtatc attatccagc agggccggag
     4261 tcagccagtt cttattttca gcgggtcgtg ccaaggttgc cgcccccgaa cctcttgccg
     4321 cgagctgtct ggcggaggcc tgcgcatcct gttcggcttc catagcctct aattttttat
     4381 ccaggttttt ttcctcggcg gaagtttcgc gaggaacaaa cagattctgg ttatggagca
     4441 gcgcaacatc tgaatagacg tggcgctggg ctttcttctt ttgcgcctca agcgaggcgt
     4501 ccagatcatc agcaaacgaa aaagcggtac aggcggccgt taccgtcagc agaacgaaaa
     4561 atctgtaata aacccgctcc atagaggttt tcaacatacg cctcccccca catcccgtca
     4621 accgccggat tagaccccgc cggttgccat ctgttcagcc tcctgctact ctccttgccg
     4681 tgcgcaaaat cttccagaca gccttcttag ccctttgcac gacggcggct tccgtgccgg
     4741 ccgttgtatc tgaattccgc ctcgaccaga accgtctctg gctcgccgcc gaaaatgaac
     4801 cgcttccgca gcttctcgga catttcgccg ccgccggaat cgacgtacag attgatcccg
     4861 acgcgcagaa aaccgtcacc ggctcctgcc gcgccgccga tgttgaagag gttctcgaca
     4921 aactgctcac gccctacagc tacctgctcg actggcggcg cgaacccgga ccgctcggcg
     4981 acctcatccg tctcaccgga atccgcgtct tccgccaagg gcacgcctcg gcggtacagc
     5041 cgcttcgcgc cgcgcgccgc atcgaaacct cccttgacgg acgcttccgg tatctggccc
     5101 gcgaaatcct catcggcttt aagcccggct cttctattga aaacctgcgc gctttgctgg
     5161 cccgaaccgg cggaaccgtc attgccgcca atgccgaact cggcatctac cgtattctgc
     5221 ttcccgaagg cgccaacgtc ctcgaccttg ccggccaact cggcaacgaa agcagtcttg
     5281 ctctcgccga gccgaattac gtttacgacc tgcctggtct tctgcccggc gaaaagaaca
     5341 cctctgccgc cccggctcag tggacagccc ccgacaaaag cggaaaaatc gcggtctccg
     5401 ttctcgactc cggccttttg gcggacagca gccttgaccg cgccgtcatc agcgctttcg
     5461 atgccaccag ccccggctcg ccgctcaccg ccgacgccgt cggacacggc acgctcatgg
     5521 cgcagctcgc ctccggactt cttgatccgt acaacagccc cgtcggcgaa ggcgtgccgg
     5581 ttgtcgccgt caaagccttt gatgataaag gcttagccga ctccttcacc ctcatgagcg
     5641 ccatgaccta tgccgtcaaa aacagctccg gcccggtcag cctcagctgg ggatcagaaa
     5701 cgcccagcaa atttatcgaa tccgccgtga aatacgccat ctcgcaagac cggcccgtct
     5761 tcgccgccgt cggcaacgaa aacaccggac ggccgatgta tccggcggcc tatcccggcg
     5821 tcgtcggcgt cgccgcctcg aacggcgacc ggctggccga ttactccaac cgcggcgact
     5881 ttgtggatat cattgcgccc ggttcggccg gcggctcgca gggaacctct gtagcaacgg
     5941 cttatgtagc ccacatcgcc gcgctctaca tgaaaaacca tcccggcgtt tccgccgccg
     6001 acaccgtttc cgc
//
LOCUS       DAOPZO010000112         5974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_16488_length_5974_cov_8.331982, whole genome shotgun sequence.
ACCESSION   DAOPZO010000112 DAOPZO010000000
VERSION     DAOPZO010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16488_length_5974_cov_8.331982"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="PKI68_07425"
     CDS             complement(<1..1088)
                     /locus_tag="PKI68_07425"
                     /inference="COORDINATES: protein motif:HMM:NF027806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNX53558.1"
                     /translation="MNGYLIFILALLIFNWLLSLIVETLNLRNVSTEIPEEFKGIYDT
                     EKYAKSQRYLRENTRFGQLQAAIMLPITIAFILLGGFSWINGIAQNASGSMIVQGLVF
                     GGILVLLSQLAGIPFSLYDTFVIEERYGFNKTTPKTFVVDILKGLMLTALLGAPIFAL
                     ILWIFSAVPNAWLWAWGALAVIQLFILFIAPVVILPLFNKFTPLEESELRTEIEEFAE
                     EQKYTLSGVFKIDGSRRSTKSNAYFTGFGKTKRIALFDTLIEKHTVPELIAVLAHEIG
                     HCKLGHIKKGIAISLASSLLMFFILSLFITKQGLYTAFGLEGTPLYAGFIFFGFLYAP
                     ISMILNLLGNILSRKHEFEADAFAAETTG"
     gene            1147..2550
                     /gene="leuC"
                     /locus_tag="PKI68_07430"
     CDS             1147..2550
                     /gene="leuC"
                     /locus_tag="PKI68_07430"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNX53559.1"
                     /translation="MSKTLFDKIWDAHVVKELGNGEVLLYIDRHLVHEVTSPQAFEGL
                     RLAGRKVRCPERTFATMDHNVPTDENRMNITDPIAKAQIDALTENAAETGVTLFPLGD
                     DRQGVIHIIGPEQGIVLPGMTVVCGDSHTATHGAFGSIAFGIGTSEVEHVLATQTLRQ
                     KKPKNYKVEFTGKLQPGVTAKDMVLKLIGMIGTAGGTGCAFEFCGEAIRNLSMEGRLT
                     ICNMAIEGGARAGLVAPDQVTFDYIASGDRKYAPKGEALTKAIAEWKKLYTDEGAKFD
                     YELMIDVSKLAPQVTWGTSPGMVTDVTGTVPALDAVPEYSPADVRAALDYMGLESGMK
                     MTDIQIDTVFIGSCTNGRIEDLREAAAVIKGKKVAKGVRVLVVPGSEAVRYAAEKERL
                     DRIFVQAGAEWRYAGCSMCLAMNPDKLKDKERCASTSNRNFEGRQGKGGRTHLVSPAM
                     AAAAAIHGHFVDIRELL"
     gene            2646..3260
                     /gene="leuD"
                     /locus_tag="PKI68_07435"
     CDS             2646..3260
                     /gene="leuD"
                     /locus_tag="PKI68_07435"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNX53560.1"
                     /translation="MEKFKTFTGTVAGVDRANIDTDAIIPKEYLKSIKRTGFGAALFS
                     DWRYNDDGSDNPSFVLNQPVTAGAQVLVARNNFGCGSSREHAVWAVQQYGFKVVIAPR
                     EGTIPAFADIFRGNCAKNGLLTIEMTSAQVDAIFQTLEKEPGVKGTVNLEAQQVVLHA
                     AAGDQAYAFDIDPVLKNKLVNGLDDIALTLQYADDIAAYEARHK"
     gene            3280..4314
                     /locus_tag="PKI68_07440"
     CDS             3280..4314
                     /locus_tag="PKI68_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNX53561.1"
                     /translation="MQNQRVLTFLTGVIALVLVGFVLKIAQAVVLPLVIAWFLSYILA
                     PPVNFLARHRVPTAPAVAVVMILLFGFFYLTGLFLYSRSMAFIEQYPKYADQFTQIAT
                     AVKERYTVPAWLSLGEIDWNRQIGQHLMSFSGSFIAFTGNLAMVLVFLIFMLLGKPYF
                     AGKVRRAFPDNQGQRVANVTSSIAHQISRYLYVKLILSTVSSLLVWIVLVIGKSDFPL
                     TWAVLAFLLSFIPSIGTVLASVPPVLLAFVQFYPNFWVAAGIATALLIIHQVIGCLVE
                     PKVMGDKLNLSPMVILLSLVFWGWLWGITGALLAVPIAAAIKIVCENIEMLKPAAVLM
                     GADTHHSEPL"
     gene            4330..5193
                     /gene="nadC"
                     /locus_tag="PKI68_07445"
     CDS             4330..5193
                     /gene="nadC"
                     /locus_tag="PKI68_07445"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNX53562.1"
                     /translation="MKLPDILSFPDVKQRIRDALAEDLGPGKLDATSFALVPAGETAE
                     AHLVARQDGILSGGPVAAEVFRQVDGCLEIVEKKKDGSAVKPGDVVLSIIGSARAMLV
                     AERTALNFIQRMTGIATLTRRYVEEAGNPDVLILDTRKTTPTLRTFEKYAVVCGGGTN
                     HRYGLFDRVLIKDNHLAHWTREGGDLAGSIHVTREKYPHLLVEVEVDTIQQLKSVLPA
                     KPDWVLLDNMPPSELRECVKLCAGICKTEASGGVNLKTIREIARTGVDAISVGALTHS
                     APSSDLALDFV"
     gene            5195..5929
                     /locus_tag="PKI68_07450"
     CDS             5195..5929
                     /locus_tag="PKI68_07450"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNX53563.1"
                     /translation="MTASPAILVVDIGNTSTSLAVARNLKLSSIQRTPSGEPALKLRA
                     VPERAVIASVKPGVNKRWEKFLKEQGVTDFLWVNHTVNLGVPVSYPKPETIGADRLAN
                     ACEAAADYGTPVIVCDFGTALTFDIVKKREGYVGGIICPGLPLMFDYLAEKTALLPHI
                     KPVKTTHVVGKSTEEAMRIGAHLGYLGMVKEILAQLRKEIGKGAKVCATGGFAKWVFE
                     GWDHPVPVDPQLTLKGLARIATLNKF"
BASE COUNT         1232 a         1628 c         1840 g         1274 t
ORIGIN      
        1 ccggtggttt cggcggcgaa ggcgtcggct tcgaactcgt gtttgcgcga gaggatgttg
       61 ccgagcaggt tcaagatcat gctgatgggc gcgtagagaa agccgaagaa gatgaatccg
      121 gcgtagagcg gcgtgccttc gagtccgaac gcggtgtaga gtccctgttt ggtgatgaag
      181 agcgagagga tgaagaacat cagcagcgac gaggcgagcg agatggcgat gccttttttg
      241 atgtggccga gtttgcagtg tccgatttcg tgcgccagta cggcgatgag ttccggaacg
      301 gtatgttttt cgatcagggt gtcgaagagg gcgatgcgtt tggttttgcc gaagccggtg
      361 aagtaggcgt tggatttggt ggagcggcgc gatccgtcga ttttgaagac cccgctcaag
      421 gtgtatttct gttcttcggc gaattcttcg atttcggtgc gcagttcgct ctcttcaagc
      481 ggggtgaatt tgttgaagag cggcaggatg acgaccggcg cgatgaagag gatgaagagc
      541 tggatgacgg ccagcgcgcc ccacgcccag agccaggcat tcggtacggc gctgaagatc
      601 caaaggatga gcgcgaagat cggcgcgccg agcagggcgg tgagcatcag ccctttgaga
      661 atgtcgacga cgaaggtttt cggggtggtt ttgttgaagc cgtagcgttc ttcgatgacg
      721 aaggtgtcgt agaggctgaa cgggatgccg gccagctggc tgagcaagac gaggattccg
      781 ccgaagacga gtccctgtac gatcatgctg ccggaggcgt tctgagcgat gccgttgatc
      841 cagctgaagc cgccgagcag gatgaaggcg atggtgatcg ggagcatgat ggcggcctgg
      901 agctgcccga agcgggtgtt ttcgcgcagg tagcgctgcg atttggcata tttttcggtg
      961 tcgtaaatac ccttaaactc ttccggtatt tcggtggaga cgttgcgcag gttgagggtt
     1021 tcgacgatca gcgacagcag ccagttgaaa atcagcaggg cgaggatgaa aatcaggtag
     1081 ccgttcataa tttgtgcgtc cttttgcgtt gatgggaacg ggcgtaaaaa tatatcctct
     1141 acccctatga gtaaaacact gtttgacaag atttgggatg cacacgtcgt caaggagctg
     1201 ggtaacggcg aggtgctgct gtatatcgac cgccacctgg tgcacgaagt gaccagtcct
     1261 caggcttttg aaggtctgcg tctggccggc cgcaaggtcc gctgcccgga acggacgttt
     1321 gccacgatgg accacaatgt tccgacggat gaaaaccgga tgaacatcac cgacccgatt
     1381 gccaaggcgc agattgatgc gctgacggaa aatgcggcgg aaaccggcgt tacccttttc
     1441 ccgctgggcg acgaccgcca gggcgttatt catatcatcg gcccggagca gggcattgtg
     1501 ctgcccggta tgacggtggt ttgcggcgac tcgcacacgg ccacgcacgg cgcgttcggt
     1561 tcgatcgcgt tcggtatcgg cacgtccgaa gtcgagcatg tgctggcgac gcagacgctg
     1621 cgccagaaga agccgaaaaa ctataaagtg gaatttaccg gcaaactgca gcccggcgta
     1681 accgccaagg atatggtgct gaagctgatc ggtatgatcg gcaccgccgg cggtaccggc
     1741 tgcgccttcg aattctgcgg cgaagccatc cgcaatctct ccatggaagg ccgcctgacg
     1801 atctgtaaca tggcgattga aggcggcgcg cgcgccggtc tggtcgctcc cgatcaggtg
     1861 acgtttgact acattgcctc cggcgaccgc aagtatgcgc cgaaaggcga ggcgctgacg
     1921 aaagcgattg ccgaatggaa gaagctctac accgatgaag gtgcaaaatt tgattacgag
     1981 ctgatgatcg acgtgagcaa gctggctccg caggtaacct ggggaaccag ccccggcatg
     2041 gtgaccgatg tcaccggcac ggttccggcg ctcgacgcgg ttccggaata cagcccggcc
     2101 gacgtgcgcg cggcgctgga ttatatgggc cttgagtccg gcatgaagat gaccgatatc
     2161 cagatcgaca ccgtatttat cggcagctgc accaacggac gcattgaaga tctgcgcgaa
     2221 gccgccgccg tcatcaaagg caagaaagtt gccaagggcg tgcgcgtgct ggtggttccc
     2281 ggctccgaag cggtgcgcta tgccgccgaa aaggaacggc tggaccgcat ctttgtgcag
     2341 gccggcgccg agtggcgcta tgccggttgc agcatgtgtc tggcgatgaa tcccgacaag
     2401 ctgaaggaca aggagcgctg cgcgtccacc tcgaaccgga actttgaagg acgtcagggc
     2461 aagggcggac gcacccatct ggtcagcccg gcgatggccg ccgcggcagc aattcacgga
     2521 cacttcgtgg atatccgcga attgctgtaa attcgaaaaa tagttaaccg cgaaagaacg
     2581 cagagaacac aaaagccatt cgtgtcaatt cgtgtgattc gtgggctgaa aatctggaga
     2641 acagaatgga aaagtttaaa acatttacag gcacggtcgc cggtgtggac cgcgcaaaca
     2701 ttgatacgga tgcgattatt ccgaaagagt atctgaagtc gatcaaacgg accgggttcg
     2761 gtgccgcgct gttttccgac tggcggtaca acgacgacgg ttcggataat ccgtcttttg
     2821 tgctcaacca gccggtgacg gccggagccc aggtgctggt cgcccgcaat aatttcgggt
     2881 gcggatcgag ccgcgaacac gccgtctggg cggttcagca gtacggcttc aaggtggtca
     2941 ttgctccgcg cgaaggcacc attccggctt tcgcggacat cttccgcggc aactgcgcga
     3001 aaaacggtct gctgacgatc gaaatgacct ccgcgcaggt ggatgcgatt ttccagacgc
     3061 tggagaaaga gccgggcgtg aagggcacgg tcaatctgga agcgcagcag gtggttctgc
     3121 atgccgccgc cggcgatcag gcctatgcgt tcgatatcga tccggtgctg aagaacaagc
     3181 tggtgaacgg tctggacgac atcgccttga cgcttcagta tgccgacgac atcgcggcgt
     3241 atgaagcgcg ccacaagtaa tcaattccgg aggcagacca tgcagaacca gcgcgttttg
     3301 acttttctaa cgggcgtcat cgctctggtt ctggtcggct tcgtgctgaa aattgcgcag
     3361 gcggtcgtcc tgccgctggt catcgcctgg tttctttcgt acatcctcgc accgccggtt
     3421 aactttctgg cccgccaccg cgtgccgacg gcaccggcgg tcgcggtggt tatgattctg
     3481 ctgttcggct ttttctatct gaccggtttg ttcctctaca gccgttcgat ggcctttatc
     3541 gagcagtacc cgaagtacgc cgatcagttc acgcagatcg caaccgccgt caaagagcgc
     3601 tacacggttc cggcgtggct gtcactcggc gagattgact ggaaccggca gatcgggcag
     3661 cacctgatgt ctttttccgg ctcgttcatt gcattcaccg gcaatctggc gatggtactg
     3721 gtttttttaa ttttcatgct gctcggcaag ccgtatttcg ccggaaaagt ccggcgcgct
     3781 tttcctgaca atcagggcca gcgggtcgcg aatgtgacct cctccatcgc gcaccagatc
     3841 agccgctatc tctacgtcaa actgatcctc agcaccgttt ccagtctgct ggtctggatt
     3901 gtattggtga tcggcaaatc tgactttccg ctcacgtggg cggtgctcgc gttcctgctc
     3961 agttttattc ccagcatcgg caccgttctg gcctcagttc cgccggtact gctggcattt
     4021 gttcagtttt atccgaactt ctgggtcgcc gccggcatcg ccacagccct gctgattatt
     4081 caccaggtca tcggctgcct ggttgagccg aaagtcatgg gcgacaaact caacctcagc
     4141 ccgatggtga ttctgctttc gctggttttc tggggctggc tgtggggcat caccggcgcg
     4201 ctgctggccg tgccgattgc cgccgccatt aaaatcgtct gcgaaaatat cgaaatgctg
     4261 aaaccggccg ctgtgctgat gggcgccgat acgcaccatt cagaaccctt atgattttgg
     4321 aggaaccgta tgaaactccc ggacattctt tcttttcccg acgtcaagca gcgcatccgc
     4381 gatgcgctcg ccgaagatct cggccccgga aaactggacg ccacttcgtt cgcactggtg
     4441 ccggcgggcg aaacggccga ggcacatctg gttgcgcggc aggacggcat actgtccgga
     4501 ggtccggtcg ccgccgaagt tttccggcag gtggacggct gtcttgagat cgttgaaaaa
     4561 aagaaagatg gttccgccgt caagcccggc gatgtggtac tgagtatcat cggttccgcc
     4621 cgcgccatgc tggtggccga gcgcaccgcg ctcaacttca tccagcgcat gaccggcatc
     4681 gccacgctga cccggcgtta tgttgaggaa gccggcaatc cggatgttct gattctcgat
     4741 acacgcaaaa ccacaccgac actgcggacg tttgaaaaat acgcggtcgt ctgcggcggc
     4801 ggaaccaacc accgctacgg cctgtttgac cgcgtgctga ttaaggataa tcacctggcg
     4861 cactggacgc gcgaaggcgg cgatctggcc ggttctattc acgttacgcg tgaaaaatat
     4921 ccgcatctgc tcgtggaagt tgaagtcgat acgattcagc agctcaagtc cgttcttccg
     4981 gcgaaaccgg actgggtgct gctcgacaat atgccgccgt ctgaactgcg cgaatgcgtc
     5041 aagctgtgcg ccggtatctg caaaaccgag gcatccggcg gcgtgaatct gaaaaccatc
     5101 cgcgagatcg cccggaccgg cgtggatgcc atctcggtcg gcgcgctgac ccattcggca
     5161 ccgtcctccg accttgcact ggattttgtc tgaaatgact gcgtcgcctg ccatacttgt
     5221 cgttgatatc ggcaacacca gcacgtcgct ggcggttgcg cgcaatctga agctttcttc
     5281 gatccagcgc acaccgtccg gcgagccggc tctcaagctg cgcgcggttc ctgaacgggc
     5341 ggtgattgct tcggtgaaac ccggcgtcaa caaacgctgg gagaagttcc tgaaagagca
     5401 gggcgttaca gatttcctat gggtgaatca tacggtcaat ctgggcgtgc cggtttcgta
     5461 tccgaagccg gaaaccatcg gggccgaccg gcttgccaat gcctgcgaag cggcggcgga
     5521 ttacggcacg ccggtcatcg tttgcgactt cggcacggcg ttgaccttcg acattgtcaa
     5581 aaagcgcgaa ggctacgtcg gcggcatcat ctgccccggt ctgccgctga tgtttgatta
     5641 tctggccgaa aagacggcgc tgctgccgca tatcaaaccg gttaagacca cgcacgtcgt
     5701 cgggaaaagc accgaagagg cgatgcgcat cggcgcgcat ctcggctatc tcggcatggt
     5761 gaaagagatc ctggcacagc ttcggaaaga gatcgggaaa ggcgccaaag tctgcgcgac
     5821 cggcggtttt gcgaagtggg ttttcgaagg ctgggatcac ccggttccgg ttgatccgca
     5881 actgacgctt aaaggccttg cccgtatcgc aacgctcaat aaattttaac gttttccttt
     5941 ttcaggcagt tgccttgaag cgaagcaact ttag
//
LOCUS       DAOPZO010000113         5918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_16691_length_5918_cov_9.643868, whole genome shotgun sequence.
ACCESSION   DAOPZO010000113 DAOPZO010000000
VERSION     DAOPZO010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16691_length_5918_cov_9.643868"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..972
                     /locus_tag="PKI68_07455"
     CDS             46..972
                     /locus_tag="PKI68_07455"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF004887.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-dehydropantoate 2-reductase"
                     /protein_id="HNX53564.1"
                     /translation="MKRFAIIGTGAVGGYYGALLQRAGFDVHFLLHSDYEFVKENGLT
                     VESVNGDFRLPQVHAYNDPQQMPRCDVVIVALKTTANHQLKTILLPVVKDDGVVLTLQ
                     NGLGSEEEIAAIAGSGRVLGGLCFLCSNKVGPGHIHHLDYGLITLGEYLADGLPGGIT
                     PRLERLGSAMTSAGIKIDLIGDLPTARWRKLVWNIPFNGLSVAKNCLTDALMNSPETR
                     ALCRTMMEEVAAASAVCARPIEPEFIEKMMTYTEKMEPYAPSMKLDFDRGNPMEIESI
                     YGNPIRAAKAAGVEMPETEKLYWQLVKLNPAR"
     gene            complement(1040..2629)
                     /locus_tag="PKI68_07460"
     CDS             complement(1040..2629)
                     /locus_tag="PKI68_07460"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNX53565.1"
                     /translation="MNNPLNILMVAAENDGIARCKVGGIGDVIRDIPAALADLGGRTS
                     IVTPSYGFLHELGGSTRVAEIRFPFAGHSDQAVLYRVRPRTEAAARSVHYVIDHPAFL
                     HYNAEKEQYDIYSNDPLGRPFATDATKFACFCAAVAAAIKENLFGPLNVLHLHDWHAA
                     FLLLLRKYDTGYASLKKLRTVYTIHNLALQGIRPLSGDRSSLESWYPDINFGTEIPGE
                     LRDPRWIDCINPMAVGIRLSDAVQTVSPSYAEEILMPSRPPQFFGGEGLDRDLQRAAA
                     EGRLHGILNGCEYPPARPAKKSVRELLSLVKSETAHWAARRTALSFADFTAFKTLDKL
                     EAARSKPSFIATSVSRVVDQKMLLLKAPGSDGVSGLEKILQIIGSKGLYILLGTGDRD
                     TEAFLAETAATHSNFLFLNSYSNKVADALYASGDLFLMPSSFEPCGIGQMLAMRDGQP
                     CLVHRTGGLRDTVQPGINGFGFEGETLHKQIDAMAAALKAAVQLHAEPEKWQAVCNAA
                     SDARFLWSDSAKQYIQKLYSW"
     gene            2878..4611
                     /gene="ilvB"
                     /locus_tag="PKI68_07465"
     CDS             2878..4611
                     /gene="ilvB"
                     /locus_tag="PKI68_07465"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNX53566.1"
                     /translation="MDKKVMDGGQALIRCLEAEGVEYIFGYSGGSVIPIFDAMVTTPS
                     KIKFVLVRHEQGAAHMADGYARATGKPAVTLVTSGPGATNVITGIMTAQMDSVPMVIL
                     TGQSVSWMIGKDAFQEADIFGMTMPVVKHSYLLKSTNDIPRTVREAFHIATTGRPGAV
                     LIDIPKDMSAGPFTADLHAEMDIPGYSTHLESIDVAQVKEIAEAISKSQRPVILAGHG
                     VLISGGHTALRALAEKLNAPVTTTLLGKGAFPETHKLSLGWLGMHGTAYANKAMCECD
                     LLLNIGSRFDDRIIGDPKWFCKNATVIHVDIDPAEIGKMIRPKYHCVADAKMFIDELI
                     KHVGKLNTGEWLKTLEGWKQKYPLKFKRQGSLKMQHVIDEFYKLSGGKAVVVTDVGQH
                     QMWAAQFYKTDAPNNFLSSGGAGTMGFGVPAAIGAQFGRPKDTVIAFCGDGGFQMTEF
                     ELATAALYKLPVKFVVLNNHYLGMVRQWQELFYEDRTSGVDLEGNPDFVKLADAYGIK
                     GFNLRRPGDVKRIIKAALAYNAGPCLINCEVEKTDNVFPMIPAGKRIEDMIIEAPKAG
                     GKKLDKPTGST"
     gene            4694..5194
                     /gene="ilvN"
                     /locus_tag="PKI68_07470"
     CDS             4694..5194
                     /gene="ilvN"
                     /locus_tag="PKI68_07470"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNX53567.1"
                     /translation="MINNKPIHTLSVYVTNKPGVLARIAQTFSRRGYNIESLVVSPAM
                     DGHFSRMTIGVSGAPEGLDQIIRQVSKLIDVLRCTDHTYDDAVVKEMGLIKIAIGAEN
                     RGEALQIAEHFGCKTVDLTDESLILQAVGDPAKIDALVEMIEKFKIIELVRTGKVLLS
                     RGAQTT"
     gene            5301..>5918
                     /locus_tag="PKI68_07475"
     CDS             5301..>5918
                     /locus_tag="PKI68_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53568.1"
                     /translation="MKTLLLTPPLLQPNTPYAATPILTAWLRSRGQEAVQADLSLEML
                     LKLFSNDGLAQLCRALKGTPDAAGFLALEEEYRAVIGDVIEFLQDRKPSAAKRLAKRG
                     TLPEGPYLVRAYEQEEQFGWDFRGLDRHDRARHLCSLFLDDIAEAAALLDPHFGFSRY
                     AEHLSASLPDFGKIRAVLESPCGADIPVCANGQTGMSAPQKTTLFL"
BASE COUNT         1291 a         1666 c         1728 g         1233 t
ORIGIN      
        1 accgccggtt tgccccggcg gtttttttat ggcactttcc cgccgatgaa acgttttgca
       61 attattggaa ccggcgccgt cggcggctat tacggcgcgc ttttgcagcg ggccggattc
      121 gatgtccact ttctgctgca cagcgattat gagtttgtga aagaaaacgg cctgaccgtc
      181 gaatcggtga acggcgattt ccggcttccg caggttcacg cctataacga tccgcagcag
      241 atgccgcgct gtgacgtagt gattgttgcg ctcaaaacca ccgcgaatca tcagttgaaa
      301 accattctgt tgccggtggt aaaggacgac ggcgtcgtgc tgacgctcca gaacggcctc
      361 ggctccgaag aggaaatcgc cgcgattgcc ggctccgggc gcgtactggg cgggctctgc
      421 tttctctgct ccaacaaagt cggccccggg catattcacc acctggatta cggtctgatc
      481 acgctgggcg aatacctcgc ggacggactg cccggcggca ttacgccgcg cctcgaacgg
      541 cttggttccg ccatgacgtc cgcgggtata aaaattgatc tgatcggcga cctgccgacg
      601 gcgcgctgga gaaaactggt ctggaacatt ccgtttaacg gcctgtcagt ggcgaagaac
      661 tgcctgacgg atgcgctgat gaacagcccg gaaacccggg ctctctgccg gacgatgatg
      721 gaagaagtcg cggcggcatc cgccgtctgc gcgcggccga tcgagccgga gttcatcgaa
      781 aagatgatga cgtatacgga gaagatggaa ccgtacgcgc cgagcatgaa actcgacttt
      841 gaccgcggca acccgatgga gattgaaagc atctacggca acccgatccg cgcggccaaa
      901 gcggcaggcg tcgaaatgcc ggaaaccgaa aagctctatt ggcagttggt gaaattgaat
      961 cccgcaaggt agggcgcttt cgccgaaagc gccgcgtgcc ctgaattggt caaagggcgg
     1021 acggctcggc gatccgtccc taccaggaat aaagtttctg aatgtactgc ttcgcgctgt
     1081 cggaccagag aaaacgcgcg tccgatgcgg cgttgcagac ggcctgccat ttttccggct
     1141 cggcgtgcaa ctgcacagcc gctttgagtg cggcggccat ggcatcaatc tgtttgtgaa
     1201 gcgtttcacc ttcgaatcca aacccgttga ttccgggctg gacggtgtcg cgcagtccgc
     1261 cggtccggtg gacgaggcag ggctgaccgt cgcgcatggc cagcatctgt ccgatgccgc
     1321 acggctcgaa ggagctgggc atgaggaaca gatcgccgga cgcatacagg gcgtccgcta
     1381 ctttgtttga atagctgttg agaaagagaa agttgctgtg tgttgcggcg gtttcggcga
     1441 ggaacgcttc ggtgtcgcgg tcgccggtgc cgagcaggat gtagaggcct tttgatccga
     1501 taatctgaag gattttttca agtccggaaa cgccgtcaga tcccggcgcc ttgagcaaga
     1561 gcattttctg gtcaacgacg cggctgacgc tggttgcgat aaacgacggc ttgctgcgtg
     1621 cggcttccag tttgtccagc gttttgaaag cggtgaagtc ggcgaaggat agagcggtgc
     1681 gtcgcgctgc ccagtgcgcg gtttcggatt tcaccagaga gagaagctct cggacgcttt
     1741 ttttcgccgg acgggcgggc ggatattcgc atccgtttaa aatgccgtgc agccggcctt
     1801 cggcggcggc gcgctgcagg tcgcggtcga ggccttctcc gccgaagaac tgcggcggac
     1861 ggctgggcat caggatttct tcggcgtagg acggcgaaac cgtctgaacc gcatcggaca
     1921 gccggatgcc gactgccatc gggttgatgc agtcgatcca gcgcggatcg cgcagctcgc
     1981 cgggaatttc ggtgccgaag ttaatgtccg gataccagct ttcgagagaa gagcggtcgc
     2041 cgctgagcgg gcggatgcct tgcagcgcca ggttgtgaat ggtgtaaacc gtgcgaagtt
     2101 ttttcagcga agcatagcct gtatcatatt tccgcaggag gagcaggaat gcggcatgcc
     2161 agtcgtgcag atgcaggaca ttcagcgggc cgaagaggtt ttctttaata gcggcggcaa
     2221 cggcggcgca gaagcaggcg aattttgtgg cgtcggtggc aaacggacgg cccagcggat
     2281 cgttgctgta aatgtcgtac tgctcttttt cggcgttgta atgaagaaag gccggatggt
     2341 cgatgacgta gtgaacggat cgcgcggcgg cttcggtgcg cggacggacg cggtagagta
     2401 cagcctgatc cgaatgtcct gcaaacggga accggatttc ggcgacccgg gttgagccgc
     2461 cgagctcatg aagaaagccg taagaagggg taacaatcga ggtgcgcccg cccagatcgg
     2521 cgagtgcggc ggggatgtcg cggatgacat cgccgattcc gccgactttg caccgcgcaa
     2581 tgccgtcatt ctctgccgcg accattagaa tgttcagggg attgttcatg gactgagacg
     2641 gtagcagagg gctttaccgg ctgaaagagg ctttttcggt tttgagaaac aggtgcggag
     2701 tctccgcttg ctgcaaaatt aaacaaaaat ttgcctcatt ttgagcatca cggattattt
     2761 atgtctaaaa tgaggtttat atttattgaa aaaaggaacc aattttcttg ctgatgtaaa
     2821 aatgaggcgt attttgtgta tacattttcg gagatgttgt ttttaaggag cgaaacaatg
     2881 gataaaaaag ttatggatgg cggtcaggct ctcatcaggt gtctggaagc ggaaggtgtt
     2941 gaatatatct tcggttattc cggcggttcg gtcattccga tcttcgacgc catggtcacc
     3001 acgcccagca agatcaagtt tgttctggtc cgccacgagc agggtgccgc ccacatggcc
     3061 gacggctatg cccgcgcgac cggcaagccc gcggttactc tcgtcaccag cggccccggc
     3121 gccaccaacg ttatcaccgg catcatgacc gcccagatgg actccgttcc gatggttatt
     3181 ctgaccgggc agtcggtttc atggatgatc ggcaaagacg cttttcagga ggcggatatc
     3241 ttcggtatga cgatgccggt tgttaaacac agctacctgc tcaaatccac caatgacatt
     3301 ccccgcacgg tccgcgaagc ctttcatatc gccaccaccg gacgtcccgg tgcggttctc
     3361 atcgatattc cgaaggatat gagcgccggc ccgtttacgg ccgatctgca cgccgaaatg
     3421 gatattcccg gctacagcac acatctggag tcgatcgatg tcgcgcaggt caaagaaatc
     3481 gccgaagcga tcagcaagtc gcagcggccg gtcattctgg ccggacacgg tgtactcatt
     3541 tccggcgggc ataccgctct gcgcgcgctg gccgaaaagc tgaacgcgcc ggttacgacc
     3601 acgctgctcg gtaaaggcgc tttcccggaa acgcacaagc tgtcgctcgg ctggctcggt
     3661 atgcacggta ccgcctatgc caacaaagcg atgtgcgaat gcgacctgct gctgaatatc
     3721 ggctcgcgct ttgatgaccg catcatcggc gatccgaaat ggttctgcaa aaacgccaca
     3781 gtgatccacg tcgacatcga cccggcggaa atcggtaaaa tgatccgtcc gaaatatcac
     3841 tgtgtcgccg acgcgaagat gtttatcgac gagctgatca aacacgtcgg caagctgaac
     3901 accggcgaat ggctgaaaac ccttgaaggc tggaagcaga aatatcccct caagttcaag
     3961 cgacagggca gcctcaagat gcagcacgtc atcgacgagt tctataaact gtccggcggc
     4021 aaagccgttg tcgtcaccga cgtcggccag caccagatgt gggcggcgca gttctacaag
     4081 accgacgcgc cgaacaactt cctcagctcc ggcggcgcgg gcacgatggg cttcggcgtt
     4141 ccggcggcca tcggcgcgca gttcggacgt ccgaaagaca ccgtcatcgc gttctgcggc
     4201 gacggcggct tccagatgac cgagtttgaa ctggccacgg ccgcgctcta caaactgccc
     4261 gtcaagttcg tcgttctcaa caaccactac ctcggcatgg tccgtcagtg gcaggaactc
     4321 ttctatgaag accgtaccag cggcgttgat ctcgaaggca accctgactt cgtcaaactg
     4381 gccgatgcgt atggtatcaa aggcttcaac ctgcgccgcc ccggtgacgt caaacgcatc
     4441 atcaaagccg cgctggccta caacgccgga ccgtgcctca tcaactgcga agtcgaaaaa
     4501 accgacaacg tattcccgat gattccggcc ggcaagcgca tcgaagacat gatcatcgaa
     4561 gcgccgaaag cgggcggtaa gaaactcgac aaaccgaccg gatcgacctg attttaaatt
     4621 taacagaagg aaacgaagga taaccttcgt tttccttcgt taccttctgt ggaaattttt
     4681 taaggagaaa accatgatca ataacaaacc gattcatact ctgagcgttt acgtcaccaa
     4741 caagcccggt gtgctcgccc gcatcgcgca gaccttctcg cgccgcggct ataacatcga
     4801 atcgctcgtt gtttcgcccg ccatggacgg ccatttctcg cgcatgacca tcggcgtcag
     4861 cggtgctccg gaaggcctcg accagatcat ccggcaggtc agcaagctga tcgacgtact
     4921 gcgctgcacc gaccacacct atgacgacgc cgtcgtcaaa gaaatgggac tgatcaaaat
     4981 cgccatcggc gcggaaaacc gcggcgaagc gctgcagatt gccgaacact tcggctgcaa
     5041 aaccgttgac ctgaccgatg aatcgctgat tctgcaggcg gtcggcgatc cggcgaaaat
     5101 cgacgcgctg gttgagatga tcgaaaaatt caaaatcatc gaactcgtcc gcaccggcaa
     5161 ggtattgctc tcccgcggcg cccaaacgac gtagtttttt acagaaggca acgaaggtta
     5221 acgaaggctg ttcaaccgct tcgttttcct ttgttacctt ctgttaaaat atttattcgg
     5281 tgtgatggtg tcctgaatgt atgaaaaccc ttttgttaac gccgccgctg ttgcagccga
     5341 acacgcccta tgccgccacg ccgatcctga cggcgtggct ccgttcgcgc gggcaggagg
     5401 ccgtgcaggc cgatctttcg ctcgaaatgc ttctcaagct cttttccaat gacggcctcg
     5461 cgcagctttg ccgcgcattg aaaggaacgc cggatgccgc cggatttctg gcgctcgaag
     5521 aggaataccg cgcggttatc ggcgacgtaa tagaatttct gcaggaccgt aaaccgtccg
     5581 ccgccaaacg gctggcaaag cgcggcacgc ttccggaagg gccgtacctg gttcgtgcct
     5641 acgaacagga agaacagttc ggctgggatt tccgcggact cgaccgccac gaccgtgcgc
     5701 gccacctgtg cagtctgttc ctcgacgaca tcgccgaagc cgccgcactg ctcgacccgc
     5761 acttcggctt ttcccgctac gccgagcatc tatccgcttc tctgccggat ttcgggaaaa
     5821 ttcgcgctgt tctggaatct ccatgtggcg cagacattcc tgtctgcgca aatgggcaga
     5881 caggaatgtc tgccccacaa aaaacaacgc tctttctc
//
LOCUS       DAOPZO010000114         5834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_17052_length_5834_cov_8.170964, whole genome shotgun sequence.
ACCESSION   DAOPZO010000114 DAOPZO010000000
VERSION     DAOPZO010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17052_length_5834_cov_8.170964"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="PKI68_07480"
     CDS             <1..987
                     /locus_tag="PKI68_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNX53569.1"
                     /translation="KASTAKKPASAPAAAESAKKTVNVISRDPYYGAVVIDASTGAIL
                     QEDHASAVAYPASMVKLMNLFLILDDVKAGKLRLDEPVTVTREVSKIGGRQVWLKEGE
                     VFPLEELIYAMMIHSANDAAAALAIRASGSCDAHAARMSAKARELGLRNTVFHSVHGL
                     PPGPGQAVDASSSLDMAMLAKVLLEVHPETLKYTSISTRPFREGNPVQLTSSNHLLGK
                     VPGCDGLKTGYFYAGGFSITATAQREGRRVIAVVLGSKDKNVRDAKAAELIEKGFTRL
                     PPLPPPPVIEPEPEVPVVPVTVEKKHSIVLPVLGAAVLVGGLVLFLRRRKKG"
     gene            complement(991..2556)
                     /locus_tag="PKI68_07485"
     CDS             complement(991..2556)
                     /locus_tag="PKI68_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53570.1"
                     /translation="MEQTVTGPSSHGKEIPDELRSVLISLGKGLGTVSVYGINHPSVE
                     LIVDKAFEDLQTALKNRKSISVGTFNGTLTVNEEPAVARDVPIRTLEKRLIAMKVSHL
                     ALSRGLTRIELKKLLTALCTTSDLQMKETLAGAGLDHIEMTDVKYVALREGEQKTGRG
                     AREKSGRTDSNADDISPAKVSQILAFLKGESANPDASTEIKKMLSDPERLGQMILDAA
                     AIRQSSGVTQDGESLADIMVGCLRRTFEGLRNAAEFQTTQGKVNLTKTMMLLEKTVLD
                     KIHAVVGAGHPEIDRRIFDAIREMEEEQQFEVLTAHYFDQRRKLDKVEEKLIENIQKH
                     GAEKAREFPGAADIPLKDWQRLTVKAGVETENTETGLSGVNGLDMSALAVVLEKLEGL
                     MQLENSDPVQIQAAVNTTRDGFTSYTDRIESRIQDLEGQIELRKRNPVTVEDHAEYLN
                     REELMVEVSKLTLAMLQPLTVINAAVEASRRHAEKEIQIDLLDLACDSGKRMQSLTKR
                     LVALVGYPVLEKQ"
     gene            complement(2667..4652)
                     /gene="tkt"
                     /locus_tag="PKI68_07490"
     CDS             complement(2667..4652)
                     /gene="tkt"
                     /locus_tag="PKI68_07490"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNX53571.1"
                     /translation="MDLKLIANTIRGLSMDGVQAANSGHPGLPLGMADVAAVVWTQFL
                     KYNPQNPQWADRDRFILSAGHGSMLIYSLLHLAGYNLPIDELKRFRQWGSKTPGHPEY
                     GHTAGVETTTGPLGQGCGNAVGMAIAEKMLAARFNSKAHKLVDHFTYVFASEGEFMEG
                     VSHEVFSMAGNLGLGKLIVFYDQNFISIEGDTHITYTDDVKKRMTAYGWHVQEINGHN
                     TDEIAAATKAAQAQTRKPSVIICNTTIGFGSPNKAGSHDCHGAPLGEEEVKLTKAALG
                     ISPEKFFVPAEVRAAFADVAKKNAAIEADWNAAFTAFEQAKPKKAKEWIACQNGELPA
                     NLAKKIAGFEAGGSIATRAASGKVLQDLAKAVPHLVGGSADLAPSNNTYLKDLGDISR
                     KSFKGRNFHFGVRELGMGAVMNGVQLHGGFRIYGATFLVFADFVRPAMRLAALMNLPV
                     IYVYTHDSFYVGEDGPTHQPIETLMSMRIIPNMTVIRPSDATETKCAWIAALENKKGP
                     TALLLTRQNLPIFDRKELAPACGLKKGAYTIWESAKSGHDIVLIAGGSEVSIALEAGR
                     KLAAEGKTVRVVSFPSWELFEAQDADYKASVFPPACTCRLAVETGATQGWEKYTGAQG
                     RVIGINHFGASAPAGTLAKEFGFTVENIYKTAREMLG"
     gene            4783..5616
                     /locus_tag="PKI68_07495"
     CDS             4783..5616
                     /locus_tag="PKI68_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNX53572.1"
                     /translation="MIDLHSHSIFSDGSSSPEELIALAEQGGLTALALTDHDTTDGLV
                     RFMAAGRNTSVQTVAGIELSAEFGEVTFHILGYLFDSAHEELQTALKWVREGRTERNV
                     RILEKLNRLGYNLTHDDVRKHAGDELVGRPHFAAALIEKGHFKHKDKIFQQLLGKGKA
                     AYAERRRLTPEACVEIIRKAGGVPVIAHPGQMKLTSRNLRRLVKKLKEHGLGGIEVWH
                     PIHHPHQVVSYLSICEDFDLVATGGSDFHGSLTPDLTLGRGFGDLDIPASALENLRNR
                     I"
     gene            5613..>5834
                     /locus_tag="PKI68_07500"
     CDS             5613..>5834
                     /locus_tag="PKI68_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HNX53573.1"
                     /translation="MKVVIAEKPSVARELAAVLNITTKKNGYIEGRGCAITWAFGHLV
                     ALQEPGDYDSALKRWSLESLPFIPDEFKLK"
BASE COUNT         1220 a         1580 c         1643 g         1391 t
ORIGIN      
        1 aaagcgagta cggcaaagaa gccggcttcc gctccggcag cagcggaatc ggcaaagaaa
       61 accgtaaacg tcatttcacg cgatccgtac tacggtgctg ttgtgattga tgcgtcaacc
      121 ggtgcgattc ttcaagaaga ccatgcatct gcagtggcct atccggccag catggtgaag
      181 ctgatgaatc tgtttctgat tcttgatgat gtaaaagccg gcaagcttcg tctggatgaa
      241 ccggtgacag tgactcgcga ggtgtcaaaa atcggcggcc gtcaggtctg gttgaaggaa
      301 ggcgaagttt tcccgcttga agaactgatt tatgcaatga tgattcactc ggccaatgat
      361 gcagcggcgg ctttggcgat tcgcgcttcc ggcagctgcg atgctcacgc ggcgcggatg
      421 agcgccaaag cgcgcgagct tggcttgcgc aatacggttt ttcacagcgt acacggtctt
      481 cctccggggc cggggcaggc ggtcgatgca agctcgtcac tggatatggc catgctggcc
      541 aaggtgttgc tggaggtgca tcctgaaacg ctgaagtata cttcaatttc cacccgtccg
      601 ttccgcgaag gaaatccggt acagctgacg agctccaatc acctgcttgg caaggtgccg
      661 ggatgcgacg ggctcaaaac gggttatttt tacgccggcg gattttccat tacggccacc
      721 gcgcaacgtg aaggtcgccg cgtgatcgcg gtcgttctgg gctcaaagga taaaaacgta
      781 cgcgatgcca aagccgctga actgattgaa aaaggattca cgcgcctccc gccgctgccg
      841 cctccgcctg ttatcgagcc ggaacctgaa gttcctgttg ttcctgtcac agtggagaaa
      901 aaacacagca tcgttctgcc tgtactcggc gcggccgtgc ttgtcggcgg actggtactg
      961 tttctccgcc gccggaaaaa aggataaacg ttactgtttc tccagaaccg gatagccaac
     1021 gagtgcaacc agacgcttcg tgagcgattg catccgtttg ccgctgtcac acgccaggtc
     1081 gagaagatca atctgaattt ccttctcggc gtggcgccgg gaggcctcga cggcggcgtt
     1141 gataacagtg agcggctgga gcatggctag tgtcagcttg gatacttcaa ccatcaattc
     1201 ttcacggttc aaatattcag cgtgatcttc aacggttacc ggattgcgtt tgcgcagttc
     1261 aatctggcct tcaaggtcct gaatgcggga ctcaatgcgg tctgtatagg aggtgaatcc
     1321 gtcgcgcgtg gtattgacgg cggcctgaat ctgaacggga tcgctgtttt caagctgcat
     1381 caatccttcc agcttttcga gaacaacggc cagtgcgctc atgtccaaac cgttaacgcc
     1441 tgacaaaccg gtttctgtat tttcagtttc aactccggcc ttaaccgtca acctctgcca
     1501 gtctttgagc ggaatatccg cagcccctgg aaattcgcgg gctttttctg cgccgtgttt
     1561 ctgaatattt tcgatcagtt tttcttccac tttatccagt ttgcgccgct ggtcgaagta
     1621 gtgggccgtg agtacttcaa actgctgttc ttcttccatt tcacggatgg cgtcaaagat
     1681 acggcggtca atctcaggat gtcctgctcc gaccacagcg tggattttat ccagtacggt
     1741 tttttccagc agcatcatcg ttttggtcag attcactttt ccctgagtgg tctggaattc
     1801 agctgcgttg cgcaatccct caaaggtacg gcgcagacag ccgaccataa tatccgcgag
     1861 tgattcaccg tcctgagtga caccggacga ctgccggatg gctgcagcat ccagaatcat
     1921 ctgtcccaac cgctcggggt cggagagcat cttcttgatt tcggtagaag cgtcgggatt
     1981 ggcagattca ccttttagaa aggcaaggat ctggctgacc ttcgccggag agatgtcatc
     2041 tgcatttgaa tccgtgcgcc cgcttttctc ccgcgcgcct cttccggttt tctgttctcc
     2101 ttcgcgcaat gcgacgtatt tgacgtccgt catttcgata tgatccagtc ccgcgccggc
     2161 aagcgtttcc ttcatttgca aatcactggt tgtgcagagc gccgtgagca gttttttcag
     2221 ctcgatgcgg gtcaaccccc ggctgagtgc aagatgagag accttcatgg cgatcagtcg
     2281 cttttcgagc gtgcggatcg gtacatcacg cgcaacggcc ggttcttcgt taaccgtcaa
     2341 ggtgccgttg aatgtgccga ctgaaattga ttttcgattt tttaaagccg tctgaaggtc
     2401 ctcaaaggcc ttgtcgacga tcagttcaac agaaggatgg ttgatgccgt aaaccgagac
     2461 ggtgcccaaa ccttttccca gtgaaataag aactgagcgg agttcatcgg gaatttcttt
     2521 gccgtgcgat gaggggcctg taactgtctg ctccataata aactctcctt gccacggtac
     2581 tctaatgcca acgaggactt cttcccagtg taaaaataaa aagctcaccg gtgaaggtga
     2641 gctttacgag ccggtggaag ccgggcttaa cccagcattt ccctggcggt tttatagatg
     2701 ttttccaccg tgaacccgaa ctcttttgcc agcgtaccgg ccggcgcgga agcaccgaaa
     2761 tgattgatgc cgatcacgcg tccctgtgct ccggtgtatt tctcccagcc ttgtgtcgcg
     2821 ccggtttcaa ccgccagacg gcaggtgcag gcaggcggga atactgatgc tttgtaatcg
     2881 gcatcctgag cctcgaacag ctcccagctc gggaagctga caacgcgcac ggttttgcct
     2941 tccgccgcca gtttcctgcc ggcttcaagc gcaatggaaa cttcggaacc gccggcgatg
     3001 agaacaatat cgtggccgct ctttgcgctt tcccagatgg tatacgcgcc tttcttaaga
     3061 ccgcatgccg gagccagttc cttgcggtca aagatcggca gattctggcg ggtcagcagc
     3121 agggcggtcg gccctttttt gttttcaagc gcggcgatcc acgcacactt ggtttcggtg
     3181 gcgtcggacg ggcggatgac ggtcatgttg ggaataatcc gcatgctcat cagcgtttca
     3241 atcggctggt gagtcgggcc gtcttcgccg acatagaagc tgtcgtgcgt gtaaacgtag
     3301 atgaccggca ggttcattag tgcagcgaga cgcatggccg ggcgcacaaa atcggcaaag
     3361 accaggaagg tggccccgta gatgcggaaa ccgccgtgca gctggacgcc attcattaca
     3421 gcgcccatgc ccagttcgcg cactccgaag tgaaagttcc ggcctttgaa ggattttctg
     3481 ctgatgtcgc ccaaatcttt cagataggta ttattgctcg gtgcaaggtc agctgacccg
     3541 ccgacgagat gcggaaccgc tttggcgaga tcctggagta ccttgccgga agcggcgcgg
     3601 gtggcaatcg atccgccggc ttcgaagccg gcgatttttt tggcgaggtt ggccgggagt
     3661 tcgccgttct gacaggcgat ccactccttg gcttttttcg gtttggcctg ttcaaaggcg
     3721 gtgaaagccg cgttccagtc tgcctcgatt gcggcgttct ttttcgcgac gtcggcaaag
     3781 gcagcgcgca cttccgcggg aacaaaaaac ttttcgggcg agatgccgag cgcggctttg
     3841 gtgagtttta cttcttcttc tccgagcggt gcgccgtggc agtcgtgcga gcccgcttta
     3901 ttcggcgagc cgaacccgat cgtggtgtta cagataatga ccgacggttt gcgcgtttgc
     3961 gcctgtgcag ctttggtggc ggcggcaatt tcgtccgtgt tgtgtccgtt gatttcctga
     4021 acgtgccagc cgtaggctgt catgcgtttt ttgacatcgt cggtgtaggt gatatgcgtg
     4081 tcgccttcga tgctgatgaa gttctggtcg tagaacacga tcagtttgcc cagtccgagg
     4141 ttgcctgcca tcgagaatac ttcgtgcgaa acgccttcca tgaactcgcc ttcgctggcg
     4201 aacacatagg tgaagtgatc gaccagttta tgagccttgc tgttgaagcg cgctgccagc
     4261 attttttcgg cgattgccat accgaccgcg ttgccgcagc cctgaccgag cgggccggtg
     4321 gtggtttcga caccggcagt atgaccatac tccggatggc cgggagtttt acttccccac
     4381 tggcggaagc gtttcagttc gtcgatcggc agattgtaac cggcgaggtg cagcaggctg
     4441 taaatcagca tcgagccgtg gcctgcggag agaataaagc ggtcgcggtc cgcccactgc
     4501 ggattctgcg gattgtattt aaggaactgt gtccagacaa ccgccgcgac atccgccata
     4561 cctaagggga gcccgggatg gcctgaattg gcggcctgca caccgtccat tgaaagtccg
     4621 cgaattgtat tggcaatcag cttgagatcc atttttgctc cttttggtcg tttaatccgg
     4681 cgatattcct ctaaaatcct cgcgaactta gcgaaaacca tgtccaatga ctaataggaa
     4741 ttgaatgaat tttgaaacga aagagaaccg aaagccggcc gcatgattga tttacattca
     4801 cactcgattt tttccgacgg ttccagcagt ccggaagaac tgattgcact ggcggaacag
     4861 ggcggactga ccgcgctggc gctgaccgat cacgacacca ccgacgggct ggttcgtttc
     4921 atggccgccg gccggaacac gtctgttcag acggtcgccg gcatcgaact cagcgcggaa
     4981 ttcggcgaag tcactttcca tattctcggc taccttttcg attctgcgca tgaggaattg
     5041 cagaccgcac tcaaatgggt gcgggaaggg cgcaccgagc ggaatgtccg gatattggaa
     5101 aagctcaatc gcctgggcta caatctcact cacgacgacg ttcgcaaaca tgccggcgac
     5161 gagttggtcg gccgtccgca tttcgcagcg gcgctgatcg aaaaagggca ctttaaacac
     5221 aaagacaaaa tttttcagca gctgctcggc aaaggaaaag ccgcgtatgc ggaacggcgg
     5281 cggcttacac ccgaagcatg tgttgagatc attcgcaaag cgggcggcgt accggttatt
     5341 gcgcatccgg gacagatgaa actcacctcg cgcaacctgc gccggctcgt caaaaaactg
     5401 aaagaacacg gccttggcgg aatcgaagtc tggcatccga ttcaccatcc gcatcaggtc
     5461 gtttcctacc tgagcatctg cgaagatttt gatctcgtcg ccaccggcgg cagtgatttc
     5521 cacggcagtc tcacgccgga cctcacgctc ggccgaggct tcggcgacct cgacattccg
     5581 gcttcggcac tcgaaaatct gcgcaaccgc atatgaaagt tgtcattgca gaaaaaccgt
     5641 ccgtcgcccg cgaactcgcg gcggtgctga atatcacgac gaaaaagaac ggttatattg
     5701 aagggcgcgg ttgcgcgatc acctgggcgt tcgggcatct ggtggctttg caggaaccgg
     5761 gcgactatga ttcggcgctg aaacgctggt cgctggaatc gcttccgttt attccggacg
     5821 aatttaaatt aaag
//
LOCUS       DAOPZO010000115         5783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_17271_length_5783_cov_11.151362, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000115 DAOPZO010000000
VERSION     DAOPZO010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17271_length_5783_cov_11.151362"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..1491)
                     /locus_tag="PKI68_07505"
     CDS             complement(25..1491)
                     /locus_tag="PKI68_07505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNX53574.1"
                     /translation="MGKPTGFKEFERKTPAERPIVERIKDYNEVYRPFPEDKLREQAA
                     RCMNCGVPFCHIGCPLGNIIPEFNDLTYKGHWKKAWEILSATNNFPEFTGRLCPAPCE
                     EACVLGINEPPVTIENIEKEIIEHAFREEWVLPVIPETRTGKTVAVIGSGPAGLAAAQ
                     QLNRAGHTVTVFERADRAGGLLRYGIPDFKLEKWVIDRRVEVMKAEGIVFKTGAAVGT
                     DISADELKQFDAVVLAIGSTVGRDMPVLGRDLKGIHFAMEFLPLQNKTCAGDYPEPLI
                     SAKGKNVVVIGGGDTGSDCVGTSIRHGARSVVNFELFPEPPSGRPPHQPWPYWPMKLR
                     TSSSHREAGEDPRRYCMLTKEFNGQNGHVTSVRTVNVEFTRERQTGKSNMEEVPGSQK
                     DWQADLVLLALGFTGPERDNAVKALGVELDKAGNIKTAKNYQTSVPNVFAAGDCRRGQ
                     SLIVWAISEGRETARCVDEFLMGETSLPTRGTAELPRR"
     gene            complement(1534..1902)
                     /locus_tag="PKI68_07510"
     CDS             complement(1534..1902)
                     /locus_tag="PKI68_07510"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNX53575.1"
                     /translation="MRDHRKLKAFELADALALSVYQQTRNFPKEEIYGLTSQIRRAAV
                     SVSSNIVEGCAQNTEKDYIHFLDTAYGSAKEVEYQTSIARRLGYLGTNKLEEDAAEVA
                     RVLNALINALRNGGGEQSKA"
     gene            complement(1947..>5783)
                     /gene="gltB"
                     /locus_tag="PKI68_07515"
     CDS             complement(1947..>5783)
                     /gene="gltB"
                     /locus_tag="PKI68_07515"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HNX53576.1"
                     /translation="LVHQRYSTNTFPAWKLAQPFHVLCHNGEINTLRGNFNWMNARQG
                     LLKSRLFGENIDRLFPIIQPGLSDSASLDQAVELLYHGGRSLPHIMSMLIPEAWQNHK
                     TMSAEKKAFYEYHSCLMEPWDGPASIPFTDGTCLGAILDRNGLRPSRYIVTKDGYVIM
                     GSETGIMDIDPANVERKGRLEPGRMFLVDMEKGRIVPDEEIKHEIAAGQPYEKWLKEN
                     LVPLSSLPPEEAAGCDPETLLQRERMFGYTMEDLNLILAPMAKEGAEPSGSMGIDTPL
                     AVLAEKPQLLYNYFKQLFAQVTNPPLDAIREELVTSLVTNIGAEQDLFAETPKHCSQL
                     KLDQPILTNDELARIRKLDRGNLKAVTLPMLYRLADGGAGLRTALEKLCADASQAIEN
                     GYSLLILSDRQADQENAPIPALLATAAVHNHLIRKLQRTRCGLIIESGEPREVHHFAL
                     LIGYGAGAVNPYMVGQILDDMIKQELLTGIEWKYAAKNFVKSINKGLLKVMSKMGIST
                     LHSYRGAQIFEAVGLNSSVIDTYFAGTASRIGGIDLDGIAREVSARHQAAFPKRIADK
                     TLPLDIGGAYKWRRNGEDHLLNPLAIAKLQESVRTDNAERFKEFSDLVNHDNRTTYTL
                     RGLFEFKKDRQPVPLSEVEPWTAIVKRFKTGAMSYGSISQEAHETLAIAMNRIGGKSN
                     CGEGGEDEGRFFPDENGDLRRSAIKQVASGRFGVTSNYLTNADEIQIKMAQGAKPGEG
                     GQLPGHKVKPWIAKTRYSTPYVTLISPPPHHDIYSIEDLAQLIYDLKSANPSAAVTVK
                     LVSEVGVGTVAAGVAKGKADLVLISGYDGGTGASPQSSIKHAGLPWELGLAEAQQTLI
                     LNKLRSRIRVECDGKLLTGRDVAVAALLGAEEFGFSTGPLITLGCIMMRVCHLNTCPV
                     GVATQDPELRKKFCGKPEYVINFFRFVAEELRQIMAELGFRTMDEMVGHRELLDVREA
                     VENWKAKGLDFSKILYQPVTEASTPMRCTEKQSNDLTDALDHELIRQSKPALERGERV
                     VIHTQVRNVHRAVGATLSYEISKRYGERGLPADRITIHATGSAGQSFGAFSAQGITFR
                     VTGDANDYFGKGLSGAKLIIRPPENATFVAEENILIGNVAFYGATRGEAYIRGLAGER
                     FCVRNSGVYAVVEGIGDHGCEYMTGGRVIVLGPTGRNFAAGMSGGIAYVLDEAGDFIE
                     NRCNPEMVDFDPLTNEDVAFLCERLEKQVEYTGSAVAQRLLDNWQDAVKKFVKVIPVD
                     YKRALQRIADEKRTGE"
BASE COUNT         1030 a         1575 c         1823 g         1355 t
ORIGIN      
        1 gcaagcggat ctgccggatg cttcttaccg gcgcggaagt tccgcggtgc cgcgggtcgg
       61 caggctggtt tctcccatca gaaactcatc cacgcagcgt gcggtttcgc ggccttcgga
      121 aatcgcccag acgatgagcg actgtccgcg gcggcagtcg ccggcggcaa agacgttcgg
      181 aacggaagtc tgatagttct tcgcggtctt gatattaccc gctttgtcga gttcaacgcc
      241 gagagctttg accgcgttgt cgcgttcggg gccggtgaag ccaagcgcca gcaggacaag
      301 atcggcctgc cagtctttct gcgagccggg aacttcttcc atattggatt ttccggtctg
      361 gcgctcgcgg gtgaattcga cattgacggt gcgtacgctg gtgacgtgtc cgttctgtcc
      421 gttgaattct ttggtgagca tgcagtagcg gcggggatct tcgcccgctt cgcggtgcga
      481 ggaactggta cgcagcttca tcggccagta gggccacggc tggtgcggcg gacggccgga
      541 cggcggttcc gggaacagtt cgaagttgac gacgctgcgt gcgccgtgac ggatggaggt
      601 tccgacgcag tcgcttccgg tatcgccgcc gccgatgacg acgacgtttt tgcctttagc
      661 ggagatcagc ggctccggat aatcaccggc gcaggttttg ttttgcagcg gcaggaattc
      721 catcgcgaag tgaatccctt tgaggtcgcg gcccagtacc ggcatatcgc ggccgacggt
      781 tgaaccgata gcgagcacga cggcatcgaa ctgtttcagt tcgtcggcgg agatatcggt
      841 gccgaccgca gcgccggttt taaaaacaat gccttcggct ttcatcactt cgacgcggcg
      901 gtcgatgacc catttctcga gtttgaaatc gggaatgccg tagcgcagca gtccgccggc
      961 gcggtcagcg cgttcgaata cggtgacggt atgtccggcg cggttaagct gctgtgccgc
     1021 ggccagtccc gccggaccgg aaccgatgac ggcgacggtt tttccggtgc gggtttccgg
     1081 aattacaggg agaacccatt cttcacggaa agcgtgttcg atgatttcct tctcaatgtt
     1141 ttcgatggtg accggcggtt cgttgatgcc gagtacgcag gcttcttcgc acggagcggg
     1201 gcagagccgt ccggtgaatt ccgggaagtt gttggtggcg gaaaggattt cccacgcctt
     1261 tttccagtgg cctttgtagg tgaggtcgtt gaattcagga atgatatttc cgagcgggca
     1321 gccaatgtgg cagaacggaa cgccgcagtt catacagcgt gcggcctgct cccgaagctt
     1381 gtcttcgggg aacgggcgat agacttcatt atagtccttg atgcgctcaa cgatcggccg
     1441 ttcagccggt gtcttgcgtt cgaattcctt aaaaccagtt ggcttaccca tatcgtctcc
     1501 ttcggagacg gagaaggctt tagaccttag gctttaggcc ttggactgct ctccgccacc
     1561 attccttaat gcatttatca aagcgtttaa tactcgcgcg acttccgccg catcttcttc
     1621 cagtttgttc gttcccaaat atccgagccg acgggcaatc gatgtctgat attcaacttc
     1681 cttcgccgat ccgtaggcgg tgtccagaaa gtggatgtaa tccttttcag tattctgcgc
     1741 gcaaccttca acaatattag acgaaaccga aaccgcagcc cgtcggattt gtgacgtcag
     1801 tccgtaaatt tcctccttcg gaaagttccg cgtttgctga tacacagaca atgccagtgc
     1861 atcggccaat tcaaacgcct ttaacttcct atggtctctc atgcactctt cctaaagtct
     1921 aaggtctaaa gcctatagcc tttttctcac tctccggttc tcttctcatc ggcgatgcgc
     1981 tgcagcgcgc gcttgtagtc caccgggatg actttgacga attttttcac ggcatcctgc
     2041 cagttgtcga gcaggcgctg cgctacggcg cttccggtgt attccacctg tttttcaagg
     2101 cgttcgcaca ggaaggcgac gtcttcgttg gtgagcggat caaagtcgac catttccggg
     2161 ttgcagcggt tttcaataaa gtcgccggct tcatccagca cataggcgat cccgccgctc
     2221 atcccggcgg caaagttgcg gccggtcggg ccgagcacga tcacgcgtcc gccggtcatg
     2281 tattcacaac cgtggtcgcc gatgccttcc accacggcat agacgccgct gttgcggacg
     2341 cagaagcgtt cgccggccag accgcgaatg taggcttcgc cgcgcgtggc gccgtagaaa
     2401 gcgacgttgc cgatgagaat gttttcttct gcaacgaaag tggcgttttc cggcgggcgg
     2461 atgatgagct tcgcgccgga aagtcctttg ccgaagtaat cgttcgcgtc gccggtgaca
     2521 cggaaggtga tgccctgtgc gctgaacgcg ccgaagctct gtccggccga gccggtcgcg
     2581 tgtatggtga tccggtcggc gggcagaccg cgttcaccgt agcgcttgga aatttcatag
     2641 ctgagtgtgg caccgacggc gcggtggacg ttgcggacct gggtgtgaat gaccacccgt
     2701 tcgccgcgtt ccagcgcggg tttggactgg cggatcagtt cgtggtcgag cgcatcggtc
     2761 aggtcgttgc tttgcttttc ggtgcaacgc atcggcgtcg aggcctctgt gacaggctga
     2821 taaaggatct tcgagaaatc gagtcctttg gctttccagt tttcaaccgc ttcgcggacg
     2881 tcgagcagtt cgcggtgtcc gaccatttcg tccatcgtgc ggaatccgag ttccgccatg
     2941 atctgacgca gttcttcggc gacaaagcgg aagaagttaa tgacatattc cggtttgccg
     3001 cagaatttct tgcgcagttc aggatcctgt gtggcgacgc cgacggggca ggtgttcaga
     3061 tggcagacgc gcatcatgat acagcccagc gtaatcagcg ggccggtcga gaaaccgaat
     3121 tcttcagcgc cgagcagtgc ggcgacggcg acgtcgcggc cggtcagcag tttgccgtcg
     3181 cactcaacgc ggatgcggct gcgcagtttg ttcaggatca gcgtctgctg ggcttcggcc
     3241 agaccgagtt cccacggcag accggcatgc ttgatggagg attgcggcga agcgccggtt
     3301 ccgccgtcgt acccgctgat gagaacgaga tcggctttac ctttggctac gccggcggca
     3361 acggtgccga cgccgacttc ggaaaccagt ttaacggtta cggcggccga cgggttggcg
     3421 ctcttcaggt cgtagatgag ctgcgcgagg tcttcgatgg agtaaatgtc gtgatgcggc
     3481 ggcggcgaga tcagcgtcac atacggagtg gaatagcgcg ttttggcgat ccacggtttg
     3541 actttatggc cgggcagctg tccgccttcg ccgggcttgg cgccctgcgc catcttgatc
     3601 tggatttcgt cggcgttcgt cagatagttg ctggtgacgc cgaagcggcc ggaagccacc
     3661 tgtttaatag ccgaacggcg caggtcgccg ttttcatccg gaaagaagcg gccttcatct
     3721 tcgccgcctt cgccgcagtt gctcttgccg ccgatgcggt tcatggcaat cgcgagcgtt
     3781 tcgtgcgctt cctggctgat ggagccgtag gacatggcgc cggttttgaa gcgtttgacg
     3841 atggcggtcc acggttcgac ttcgctgagc ggaaccggct ggcggtcttt cttgaactca
     3901 aagagaccgc gcagggtgta tgtggtgcgg ttgtcgtggt tcaccagatc ggaaaattcc
     3961 ttgaaccgtt cggcattgtc ggtgcggacg gattcctgca gcttggcgat ggccagcggg
     4021 ttcagcagat gatcttcgcc gttgcgccgc cacttgtagg cgccgccgat atccagaggc
     4081 agggttttgt cggcgatgcg tttcgggaaa gccgcctgat ggcgcgcgct gacttcgcgg
     4141 gcgatcccgt cgaggtcaat tccgccgatc cgcgaggcgg tgccggcgaa ataggtatcg
     4201 atcacgctgc tgttcaatcc gacggcttca aaaatctgtg cgccgcgata gctgtgcagg
     4261 gtggagatac ccatcttgga catgactttg agcagtccct tgttgatgga tttgacaaaa
     4321 ttcttggcgg catatttcca ttcaatgccg gtgagcagtt cctgtttgat catgtcgtcc
     4381 aggatctggc cgaccatgta cggattgacc gctccggcac cgtagccgat cagcagggcg
     4441 aaatgatgta cttcgcgcgg ctcgccgctt tcgataatca gtccgcaacg tgtgcgttga
     4501 agtttgcgga tcaggtggtt gtgaacggct gcggtcgcca gcagcgccgg gatcggcgca
     4561 ttttcctgat cggcctgccg gtcggaaaga atcagcagcg aatagccgtt ttcaatcgcc
     4621 tgtgacgcat cggcgcacag tttttcgaga gccgtgcgca aacctgcgcc gccgtccgca
     4681 agtctgtaga gcatcggcag ggtgacggct ttgaggttgc cgcggtcgag tttgcggatg
     4741 cgggcgagtt cgtcgttggt caggatcggc tgatcaagct tgagctggct gcaatgtttc
     4801 ggagtttcgg cgaaaaggtc ctgttcggca ccgatattgg tgacgagcga agtgaccagt
     4861 tcttcgcgga tggcatcgag cggcgggttg gtaacctgcg caaagagctg cttgaagtag
     4921 ttatagagaa gctgcggttt ttcggcgagc acggccagcg gggtgtcgat tcccattgag
     4981 ccgctcggct cggcgccttc tttggccatg ggtgcgagaa tcagattcag atcttccatc
     5041 gtgtagccga acatgcgttc gcgctgcagc agagtttccg gatcgcatcc ggcggcttct
     5101 tccggcggca ggctggagag cggaacgaga ttttctttga gccatttctc gtacggctga
     5161 ccggctgcga tctcgtgctt gatctcttcg tcgggcacga tgcggccttt ttccatatct
     5221 acaaggaaca tgcggcccgg ttcaaggcgg cctttgcgtt ccacgttggc cgggtcgata
     5281 tccatgattc cggtttcgga gcccatgata acgtagccgt ctttggtgac gatgtagcgc
     5341 gacgggcgca ggccgttgcg gtcgagaatg gcgccgaggc aggtgccgtc ggtgaacgga
     5401 atcgatgcgg ggccgtccca tggctccatc aggcaggagt ggtattcgta gaacgctttt
     5461 ttctccgcgc tcatggtttt gtggttctgc cacgcttccg gaatgagcat gctcatgatg
     5521 tgcggcagcg aacggccgcc gtggtagagc agttcaacgg cctggtcgag cgaggcggag
     5581 tcggaaaggc ccggctgaat gatcgggaag aggcggtcga tgttttcacc gaaaaggcgc
     5641 gacttcagca gcccctggcg ggcgttcatc cagttgaagt ttccgcgcag cgtattgatt
     5701 tcgccgttat ggcaaagcac atggaacggc tgggccagtt tccacgccgg gaaggtgttg
     5761 gtgctgtagc gctggtgtac gag
//
LOCUS       DAOPZO010000116         5670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_17791_length_5670_cov_8.635619, whole genome shotgun sequence.
ACCESSION   DAOPZO010000116 DAOPZO010000000
VERSION     DAOPZO010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17791_length_5670_cov_8.635619"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PKI68_07520"
     CDS             <1..192
                     /locus_tag="PKI68_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNX53577.1"
                     /translation="YHQIRAQLAAIGCPIAGDEKYGSKIKLPGGAIGLHHARLTVVHP
                     VTKAEVVIEAPYPGHWPVV"
     gene            complement(276..449)
                     /locus_tag="PKI68_07525"
     CDS             complement(276..449)
                     /locus_tag="PKI68_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX53578.1"
                     /translation="MPRIINKDECVNCGACEPVCPVSCISEVDSKRFIKASDCIDCGA
                     CEPVCPVSCIHEA"
     gene            609..>4060
                     /locus_tag="PKI68_07530"
     CDS             609..>4060
                     /locus_tag="PKI68_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNX53579.1"
                     /translation="MNKKQDPGPLGRKHLWLTAGSSLLLILILVFSWQTVLRTDRQMR
                     DDLLQQARIIVQSVNIDRIRNLSGTKDDLNSPAYVRLKNQFAAIRAAAPQCRFIYLMG
                     RKADGAVFFFVDSEPAGSADYSPPGEIYGDVPAGYVRVFNSRTAEVVGPVTDQWGQWI
                     SALMPIIDPRNDSVLAVMGMDVDARSWYREVAAKSAMPVGMMLALLGSAAVIAVARRL
                     AGRDLPDRPAQPVLRNLPAPLIGVSLVILTVLAGGIQYYRVQKTAIRHDVEHQLSSIA
                     LLKANEIANWRTERLGDAGTLSGNRSLVRDLEQFLAAPQPGLENELRDTLRNFQQYYA
                     YADILLVDTNGQVRLTLNAGPGDYSSLAAELTEALRNRKPVLTDFHTDASGAFRHLNI
                     VAPLVANSDPTRAIGAIILSYDADRFYSLVQSWPVSSRSAETQLVRRDGPDVLFLNEL
                     RYRTGAALKLRIPLTHTNAPSVMAVLGHKGIMQGKDYRGVDVLSALQPVPDSPWFLVA
                     KQDTAEVFADWRARSGAILILLFCLVGMAGAAGFIFWLRTQKNYYRTLHQSASARQKS
                     EALLAATLRSIGDGVITCDASGCVTGLNIIAEKLTGWETGEAAGRPIAEVFRIIHAGT
                     RQNAEIPVDRALRENRIIGLANHTALIARDGTELQIADSCAPIHDADGGVIGAVLVFR
                     DVTEEYRQREQLRDNETLQRALLDNLPAGVVIIDPETRVIERVNSYVAELFGAPVDHL
                     LGQRCHRLLCPADEGACPVCDMGKSVENAEREMLRADGSCLPILKTVKRIQLNGQEKL
                     LECFVDISERKQMELYRDLENQILRILNEPYSRQETVQHVFALIKKTTGFEALGMRLK
                     DGEDFPYFAQNGFSDDFLLKENSIIERDKNGELCRDCHGKVCLECTCGLVISGKADPN
                     HPLFTKGGSFWINDSAPLLDLPADQDPRWNPHNYCIHTGYATIALIPIRMQNEIVGLL
                     QINDRRKSRLSLTAVERFESIAAHIGEALMRKQAEEDVARNNARLQKLTDILQHPSDT
                     IQEFLDYSLEQVIELTGSGIGYIYHYHEDRKEFVLNTWSKDVMPSCAVANPQTCYELD
                     KTGIWGEAVRQRRPIIVNDFQATNSLKKGYPQGHVHLSNFMTVPVFRGEQIVGVVGLA
                     NKASA"
     assembly_gap    4061..4160
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4161..>5670
                     /locus_tag="PKI68_07535"
     CDS             <4161..>5670
                     /locus_tag="PKI68_07535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX53580.1"
                     /translation="EMAAQAAAANLAKSAFLANMSHEIRTPMNAVIGMTDLLMETKLD
                     AEQRDFANTIRISGEGLLSIISDILDFSKIEAGRMEIDDQDFDLIRCVEDTLDLMVSK
                     AAEKGIELTCEMGSDVPPVVRGDAGRLRQILLNLLSNALKFTHKGEVGISVSGKPSGK
                     GYQLAFAVRDTGIGIEKDKLDRIFEAFTQADTSTTRQYGGTGLGLTISRRLSELMGGC
                     LEAESIPGKGSVFHFTIQVSAARRTKTVRTDQKPFTVDHRDVLIVDDNETNLKILSAQ
                     LTRWGLKPVAFGSPADALQSIRDGRAYILMISDMQMPSMDGTMLIREVRKFRPAHELP
                     IIVLTSIGLEKPAEAMDISSYLVKPVKPALLYQNIANILQGEGGNYSEAVAVSAAQAV
                     ASPLQLLVVEDNQLNQKVALRMLSKLGYSADLAQDGVEAVEMTAVKSYDIVLMDIQMP
                     RMDGLEATRQICNRSAGGTRPFIIGMTAHAAGEERARGLDAGMDDYLVKPIQL"
BASE COUNT         1235 a         1621 c         1613 g         1101 t
ORIGIN      
        1 tatcaccaga tccgcgcgca gctggccgcc atcggctgcc cgattgccgg cgatgaaaag
       61 tacggctcca aaatcaaact gcccggcggc gcgatcgggc tgcaccatgc gcggctgacg
      121 gtggttcatc cggtgacgaa agcagaggtt gtcatcgaag cgccgtatcc cggccattgg
      181 cccgtggttt aaaaccggtg gagcgggcgc tccgcgcacg cttaaacgcg cccggagctc
      241 gcgttccacc tgaatattcc caatgctttg agatcctacg cttcgtgaat gcaactgacg
      301 gggcagacgg gttcgcaggc tccgcagtcg atgcagtcgg acgccttgat gaaacgtttg
      361 ctgtccactt ccgagatgca gcttaccggg caaaccggct cgcaggctcc gcagttaaca
      421 cattcgtctt tgttgatgat tcgcggcata gtgtcctcct gtcgattaga caataagaag
      481 aaaactaaac cgctttcaga tcaagttaaa tttcgagagc aggcgttcgc gccggcaatt
      541 attcatggct tcggcagtga aatttatgta cacttcggtt gaaaaaaaag cgggggagat
      601 cgcctgacat gaataaaaag caagacccgg ggccgctcgg gagaaaacat ctttggctga
      661 cggccgggtc ttcattgctg ctgatcctga tactggtttt cagctggcag acggtattgc
      721 gtaccgaccg gcagatgcgg gacgaccttc tccagcaggc gcgcattatc gtgcagtctg
      781 taaatattga ccgcatacgg aatctgtcgg gaacaaagga cgacctgaac agcccagcgt
      841 atgtgcggct caaaaaccag tttgccgcca tccgggcggc cgccccgcaa tgccggttca
      901 tctatctgat gggccgcaaa gcggacggcg cggttttttt cttcgtcgac agcgagcccg
      961 ccggttcggc ggattattcg ccgcccggcg aaatctacgg cgatgtcccg gcaggctacg
     1021 tccgcgtatt caacagcaga acggcggagg tcgtcggccc ggttacagat cagtggggcc
     1081 agtggatttc ggcgttgatg ccgatcatcg atccgcggaa tgattccgta ctggcggtca
     1141 tggggatgga tgtcgatgcc cgctcctggt accgggaagt ggctgctaaa tcggcgatgc
     1201 ccgttggcat gatgctggca ctcctcggca gcgccgccgt catcgccgtc gcccgccgtc
     1261 tggccggacg cgacctgccc gacagaccgg cacaacccgt actgcgcaac ctgccggcgc
     1321 cgctgatcgg cgtgtcgctc gtgatcctga ccgtgctggc cggcggtatt cagtattacc
     1381 gggtgcagaa gaccgcgatc cggcacgatg tggagcacca gctgtcctcc attgcactgc
     1441 tgaaggccaa tgaaattgca aactggcgga cggaacggct gggcgatgcc ggcacattga
     1501 gcggaaaccg gtcgctggtg cgcgatctgg agcagtttct ggcggctccg cagcccggac
     1561 ttgaaaacga actgcgcgat acactgcgga actttcagca atattatgcc tatgccgaca
     1621 tcctgctggt ggataccaac ggacaagtgc gcctgacact gaatgccggt cccggcgact
     1681 attcatcgct ggcggcagag ctgacggaag cgctgcgcaa ccggaagcct gtattgaccg
     1741 attttcacac cgatgcatcc ggcgcattcc ggcatctgaa tattgttgcg ccgcttgtgg
     1801 caaacagcga cccgacccgc gccatcggcg cgattatcct gagctacgat gccgaccggt
     1861 tttattcact ggttcagtcg tggcccgttt caagccgctc cgccgaaacc cagctggtgc
     1921 gccgcgacgg tccggatgtg ctgtttttga atgaactccg ttaccgcacc ggcgccgcac
     1981 tcaaactccg cattccctta acccatacga atgcgccctc cgtcatggcg gttctggggc
     2041 ataaaggcat catgcagggc aaggactacc gcggagtgga tgtgctgtcg gcgctccagc
     2101 cggttccgga ttccccctgg tttctggtgg ccaagcagga caccgccgaa gtgttcgccg
     2161 actggcgcgc ccgctccggc gccattctga ttctgctgtt ctgtctggtt ggcatggcag
     2221 gcgcagcggg cttcatattc tggctgcgga cccagaagaa ttattaccgg acgctgcatc
     2281 agtccgcgtc cgcacggcag aaaagcgaag cgctcctcgc ggccaccctg cgctcgatcg
     2341 gcgacggcgt gattacctgc gacgccagcg gctgtgtgac cggcctcaat atcatcgcgg
     2401 aaaagctgac gggctgggaa accggcgaag ccgccggacg gccgattgcc gaagtcttcc
     2461 gcattatcca cgccggaacg cggcagaacg ctgagatacc ggtggaccgc gcgctgcgcg
     2521 aaaaccgcat catcggactg gcgaatcaca cggcactgat cgcgcgcgac ggcacagaac
     2581 ttcagattgc ggacagctgc gcgccgattc atgacgcgga cggcggcgtc atcggagccg
     2641 tactcgtttt ccgcgacgtg acggaggaat accgtcagcg cgagcagttg cgcgacaacg
     2701 aaacgctgca gcgcgctctg ctggacaatc ttcccgccgg agtggtcatc atcgatcctg
     2761 aaacccgggt gatcgagcgg gtcaacagct atgtagccga actcttcggc gcgccggtgg
     2821 atcatctgct gggacagcgg tgccaccggc tgctgtgccc ggccgatgaa ggcgcctgtc
     2881 cggtctgcga tatgggaaaa tccgtggaga atgccgaacg ggaaatgctg cgggctgacg
     2941 gcagctgcct gccgattctt aaaaccgtca aaagaattca gctgaacggt caggaaaagc
     3001 tgctggaatg ctttgtggat atttccgagc gcaaacagat ggagctctat cgcgatctgg
     3061 aaaaccaaat cctgcggatt ctcaacgagc cctattcccg gcaggaaacg gtacagcacg
     3121 ttttcgctct gatcaaaaaa acgaccggat tcgaagcgct gggcatgcgg ctgaaggacg
     3181 gcgaggattt cccctacttc gcccagaatg gtttctccga tgacttcctg ctcaaggaga
     3241 attcaattat cgagcgtgat aaaaacggcg aactgtgccg cgactgccac ggaaaagtct
     3301 gtctggaatg cacctgcggg ctggtgattt ccggcaaagc cgacccgaac catccgctgt
     3361 tcaccaaagg cggcagcttc tggatcaatg attcggcccc gctgctggat cttcccgccg
     3421 atcaggatcc gcgctggaat ccgcataact attgcattca tacgggatac gccaccatcg
     3481 cgctcatccc gatccgcatg cagaatgaaa tcgtcggcct gctccagatc aacgaccgcc
     3541 ggaagagccg tctttcgctc acggctgtag agcggtttga aagtatcgcc gcacatatcg
     3601 gcgaagcgct gatgcgcaaa caggcggaag aagatgtcgc acggaacaat gcacggctgc
     3661 aaaagctgac cgatattctg cagcatccgt ccgataccat tcaggagttt ctcgattatt
     3721 cactggagca ggtcatcgaa ttgaccggca gcggaatcgg ctacatctat cactaccatg
     3781 aagaccgcaa agagtttgtg ctcaacacct ggtcaaaaga tgtcatgccg tcatgcgccg
     3841 tggccaatcc gcaaacctgc tacgagctgg ataaaaccgg tatctggggt gaagccgtcc
     3901 ggcagcgccg cccgattatt gttaacgact ttcaggcgac aaactcctta aagaaaggct
     3961 atccgcaggg ccatgtccac ctttccaatt tcatgacagt tccggttttc agaggtgaac
     4021 agattgtcgg agttgtgggg ctggcaaata aagcctcggc nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn gaaatggccg cgcaggccgc agcggcgaat ctggcgaaaa
     4201 gcgcctttct ggcaaacatg agtcatgaaa tccgcacacc gatgaacgcg gtcatcggca
     4261 tgaccgatct gctgatggaa acaaagctgg atgccgaaca gcgggatttt gccaatacca
     4321 tccggatcag cggcgaagga ctgctgtcca tcatcagcga cattctggac ttctccaaaa
     4381 ttgaagcggg acggatggaa attgacgacc aggatttcga tctgatccgg tgcgttgaag
     4441 acacgcttga cctgatggtt tccaaggcgg cggaaaaagg cattgaactg acctgcgaga
     4501 tgggcagcga tgtgccgccc gttgtgcgcg gcgatgccgg acgtctgcgc cagattcttc
     4561 tgaatctgct gagcaacgcc ctcaaattta cacacaaagg agaagtcggc atttcggttt
     4621 ccggcaaacc gtccggtaaa ggttatcagc ttgccttcgc ggttcgcgat accggtatcg
     4681 gaattgaaaa agataaactc gaccggatat tcgaggcatt tacacaagcc gacacctcta
     4741 caacacgtca gtacggagga accggactcg gactgaccat cagccgccgt ctgagtgaac
     4801 tcatgggggg ctgccttgag gccgaaagca ttccgggaaa aggctcggtg ttccatttca
     4861 ccattcaggt ttccgccgcc cggcggacaa aaacggtacg caccgaccag aaaccgttta
     4921 ccgttgatca ccgcgacgtg ctgatcgttg acgacaatga aaccaatctg aaaattctgt
     4981 cggcccagct cacccgctgg gggcttaagc ccgtcgcgtt cggcagtccg gccgacgcgc
     5041 tgcaatccat ccgggatgga cgggcatata ttctgatgat cagcgacatg cagatgccgt
     5101 cgatggacgg aaccatgctg atccgggaag tccggaaatt ccgcccggcc cacgaactgc
     5161 cgattatcgt gctgacatca atcggactgg aaaaaccggc cgaggcaatg gacatctcgt
     5221 cgtatctggt caagccggtg aaaccggctt tgctctacca gaacatcgcc aatatcctgc
     5281 aaggcgaagg cggcaactac agcgaagccg ttgctgtttc cgctgcacag gctgtcgcca
     5341 gccctctgca gctgctggtc gtcgaagaca accagctcaa tcagaaagtc gcattgcgca
     5401 tgctttcaaa actcggctac agcgccgacc tggcgcagga cggagttgag gccgtggaaa
     5461 tgaccgccgt caaatcctat gacatcgttc tgatggatat ccagatgccg cgcatggacg
     5521 gattggaagc cacccggcaa atctgcaacc ggtcggccgg cggaacccgg ccgtttatta
     5581 tcggcatgac cgctcatgcc gccggcgaag aacgggcccg cggacttgac gccggaatgg
     5641 atgattatct cgtcaaaccc atccagctgg
//
LOCUS       DAOPZO010000117         5629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_17987_length_5629_cov_8.407248, whole genome shotgun sequence.
ACCESSION   DAOPZO010000117 DAOPZO010000000
VERSION     DAOPZO010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17987_length_5629_cov_8.407248"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..602)
                     /locus_tag="PKI68_07540"
     CDS             complement(147..602)
                     /locus_tag="PKI68_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53581.1"
                     /translation="MQNKLFFGVLLMSAVVSGCKVDVSDYEPKELELHRYAPKYSCEE
                     ISIKKDDEIYRFVKKLLSENREGWHPSIVHYVPQVKVRADNDMYRLNICSNILVVMYW
                     DSEKKHTTQVVKRVSSEAYDEIVALVEISLQNAGSDNGSELKEKKGSPP"
     gene            complement(713..>2783)
                     /locus_tag="PKI68_07545"
     CDS             complement(713..>2783)
                     /locus_tag="PKI68_07545"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNX53582.1"
                     /translation="KLNPRRVAMKGIPISGVRNFALMGHTGSGKTSLLDVMLNILGTN
                     ERAGSVDDKSSMADWTPEEQEHEISIWAKPFDGVYTAASGKQRRFVVIDTPGYADFVG
                     QQMAAAEITDAALIVVDAVAGIQVGTNRAWKICEKRNLPRGVVITGLDKPEASFEKAL
                     ADVKAMWGDTRCVPVVLPTTDGKSEVDILDMPTDAVPADMAATVKAIRDHLIELAAET
                     DDKMMEKYFNGEELTADEFSHGLRESVHGAHLIPVFACSAKTKIGVKETMDSISRLFP
                     SPEDYPVKCADGHEVATGEDQPFAGLVWRSVNDAFTGQLSFVRVYRGKLTPNSEVLNA
                     TKGEKERIGSILYINGKKSTDCLEAKAGDIIALPKLKNTYLNDTLCAPTEKITFKPIE
                     FPHPVAAYAVEPKTKGDEDKIGTALHRAAEEDPSIRVERNNETHETLLWGMGDMHLDV
                     TLEHIKNRSKVDITHHTPKVAYRETITGTGEGHYKHKKQSGGRGQYGECYVRVHPRVP
                     GDNEWFANKLVGGSIPNNFVPACQKGFLEGMAKGPLVGAEVSNVKVELYDGSYHDVDS
                     SEVAFKIAGSRALHEAIEKARPVLLEPIMTIAITVPDEYMGDITGILNTKRGRILGMG
                     VDEGMQVITAEVPQVETFKFCSELRSITGGRGSFELTFARYEQVAANIAQKIIAAAQK
                     DKVAVED"
     assembly_gap    2784..2883
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2884..>5629)
                     /locus_tag="PKI68_07550"
     CDS             complement(<2884..>5629)
                     /locus_tag="PKI68_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX53583.1"
                     /translation="FGAVQLPEGATLARTDALLTQAITPLRNEPGVAFVIQITGFSLM
                     GGSGENVAFFVCGLKDWSERKAPRFWNKGKQPDLQASAIQQRLLGRLSQVPGAQIYLF
                     TPPAIRGLGASSGMNIRLQAVSDNDPQKLEAVLKGFLMKLNTMPGMMHAFSTYTANTP
                     YLFMDVDRVKASLMGVDTSAIFAALQDNLGSRYVNNVNFEGQVNNVNVQADWPFRNQV
                     EDVEKIYVKNNRGDMVPLGSVLKSRITLAPRSVDRYNKFTAAAVTAVQLPFISSGEAM
                     KRVTKLAGETLPPGYTFDWTDLSYQEAKASGGSTLLIAMALIFGFLFLVAQYESWIIP
                     LPVMLSTAVASLGALAGLLFNHMYLSIYAQLGLILLVGLASKNAILIVEFSRARHEEG
                     LSIRDAAADGMKQRFRAVLMTAFTFILGVLPMVLATGAGSAARKSIGTTVFWGMLAAT
                     VGGIILIPALYVLFQTLREKSHAIRVSKSKLHLLVILLVPFLFGGCLSVGPDYKAPEL
                     PQLNGQPAELPAAWWTTLNDPELTALVDETLQKNCDLKSAVAAVRAARAQLGIARSAY
                     GPQLDANGSYTRQKASKEISKAGESSLYRGGFDASWEIDLFGGTRRSVEASLAALKAQ
                     EAGQADVQVSLAAETAQSYVRLRAGQQRLRVARSNLEIQQATYDLLKSQFESGLIGEL
                     TLQQARYNLESTRATVPALETAVEQSLNALAVLTGVLPGSLHERLGAEKEIPVASVQT
                     VTGIPADLLRRRPDVRRAERQLAAQTARIGVAKSALYPKLTLNGSIGLESLHASSFAD
                     AGNDFYSAGPGVRWAIFHTGSIRNNINVQKAGQEQALAAYEKSVLTAVQETRDALTSF
                     EKEQQRMTALDAAASAARTAAELAQERYKNGLAGFDSVLEAQRAQLAFEDQLVQSQSA
                     I"
BASE COUNT         1086 a         1590 c         1592 g         1261 t
ORIGIN      
        1 gatatgataa cgctcccgca gaagcctcac catctcccgg cgttcctccg cccagactgt
       61 ctcgctcttc ttcacggaac cctccgcgaa ctcttccaag ggttggaaaa tcaaagtctc
      121 aattccgccc atcaaaaatt actggattac ggaggcgacc cctttttctc ttttagttca
      181 gaaccgttgt cgctccctgc attctgaagt gagatttcca caagagccac gatttcatca
      241 tacgcttcgc ttgagacccg ttttacaacc tgcgtagtat gttttttctc tgaatcccaa
      301 tacatcacga caagtatatt cgaacagata ttaagacgat acatatcatt atctgccctc
      361 actttcacct gcgggacata gtgaacgatc gaggggtgcc atccttctct attttcacta
      421 agcaacttct ttacaaacct atatatctca tcatcttttt ttatactgat ctcttcgcag
      481 gaatatttgg gagcataacg gtgcagctct aactccttcg gttcataatc actaacatca
      541 actttacagc cagacactac cgctgacatc aaaagcactc cgaaaaacaa cttattttgc
      601 acaattcttc ctcccgaaaa tggtcagttc ggtaatctag taatttcatc cgtcgtatct
      661 gctccaatct ccaagagttg gaagaatttc ttccaaccct tggaaaatca gatcaatctt
      721 cgacggcgac tttgtctttc tgagcggcgg cgatgatttt ctgggcgatg ttggcggcga
      781 cctgttcgta gcgggcgaag gtgagctcga acgagccgcg tccgccggtg atggagcgca
      841 gttcggaaca gaatttgaag gtttcgacct gcggcacttc ggcggtgatg acctgcatcc
      901 cttcgtccac gcccatgccg agaatccggc cgcgcttggt gttgaggatt ccggtgatgt
      961 cgcccatgta ctcgtcgggc acggtgatgg cgatggtcat gatcggttcg agcagaaccg
     1021 gccgcgcttt ttcaatcgct tcgtgcagag cgcgcgagcc ggcgattttg aaggcgactt
     1081 ccgacgagtc cacgtcgtgg taggagccgt cgtagagttc gactttgacg ttgctgactt
     1141 cggcgccgac cagcgggcct ttggccatgc cttccagaaa tcctttctgg caggcgggaa
     1201 caaagttgtt cggaatcgaa ccgccgacga gtttgttggc gaaccattcg ttgtcgccgg
     1261 gaacgcgcgg atgaacccgc acgtagcatt cgccgtactg gccgcgtccg ccggactgct
     1321 ttttgtgttt atagtggcct tcgccggtgc cggtgatggt ttcgcggtac gcgactttcg
     1381 gcgtgtggtg agtgatgtcc actttgctgc ggtttttgat gtgttccaga gtgacgtcga
     1441 ggtgcatgtc gcccatgccc cacagcaggg tttcgtgcgt ttcattattc cgctcgacgc
     1501 gaatcgacgg atcttcttcc gccgcgcggt gcaaagccgt gccgatcttg tcctcatcgc
     1561 ctttagtctt gggttcgacg gcataggcgg ccaccggatg cggaaactca atcggcttga
     1621 aggtgatctt ttcagtcggc gcgcagagcg tgtcgttgag gtaggtgttt ttcagcttcg
     1681 gcagcgcgat gatgtcgccc gccttggctt ccagacaatc ggttgatttt ttgccgttga
     1741 tgtaaaggat gctgccgata cgttcttttt cgcctttggt ggcgttgagg acttcggagt
     1801 tcggcgtgag tttgccgcgg tagacgcgca caaagctgag ctggccggta aaggcgtcgt
     1861 tgaccgagcg ccagaccagt ccagcaaacg gctgatcttc gccggtggcg acttcgtggc
     1921 cgtccgcgca tttgaccgga taatcttccg gcgacgggaa aagccggctg atggagtcca
     1981 tcgtttcttt gacgccgatt ttcgttttgg ccgagcaggc gaaaacggga atgaggtgcg
     2041 ctccgtgcac cgattcgcgc aggccgtgcg aaaattcgtc ggcggtgagt tcttcgccgt
     2101 tgaaatattt ctccatcatt ttgtcgtcgg tttcggcggc gagctcgatg aggtggtcgc
     2161 ggatcgcttt tacggttgcg gccatatccg ccggaacggc gtcggtcggc atatcaagga
     2221 tgtccacttc ggacttgccg tcggtcgtcg gcagaacgac cggcacacac cgcgtatcgc
     2281 cccacatggc tttaacatcg gccagcgctt tttcaaaact ggcttccggc ttatcgagac
     2341 cggtgatgac gacaccgcgc ggcaggttgc gcttttcaca gatcttccac gcgcggttgg
     2401 tgccgacctg aatgccggca acggcatcga ccacaatcag cgcggcgtca gtgatttcag
     2461 cggcggccat ctgctggccg acgaagtcgg catagccggg agtgtcgatg acgacaaagc
     2521 ggcgctgctt tccgctggcg gcggtataga cgccgtcgaa gggtttcgcc cagatggaaa
     2581 tttcgtgttc ctgctcttcc ggagtccagt cggccatact gctcttgtcg tccacggagc
     2641 cggcgcgttc gttcgttcca aggatattca gcatcacatc cagcagtgat gtcttcccgc
     2701 tgcctgtgtg acccatcaat gcaaaattgc ggacgcctga gatcggaata cccttcatcg
     2761 caactctcct tggatttaat tttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnntgatcgc gctttggctc tgcacaagct gatcctcgaa tgccagctgg gcgcgctgtg
     2941 cttccagcac ggaatcgaac ccggccagcc cgtttttata acgttcctgc gccagttcgg
     3001 ccgcagtccg cgcggcggaa gcggcggcat ccagcgccgt catgcgctgc tgctcttttt
     3061 caaaggaggt cagcgcatca cgggtttcct gaaccgctgt cagcacggat ttctcgtaag
     3121 cagccagcgc ctgctcctgt ccggctttct gcacattgat gttattacgg atcgatcccg
     3181 tatggaaaat cgcccagcgg acgccggggc cggcgctgta aaaatcgttg ccggcatcgg
     3241 cgaacgaaga ggcgtgaagg gattcaagtc cgatcgaacc gttcagcgtg agtttcggat
     3301 agagagccga cttggccacg ccgatgcgcg ccgtctgcgc ggcaagctgc cgttccgctc
     3361 tgcgcacgtc cgggcggcgg cgcagcagat cggccggaat accggtgacc gtttgaacgg
     3421 aggcgacagg aatttctttt tctgcgccga gccgttcgtg cagcgagccg ggcaggacgc
     3481 cggtgagcac ggccagcgcg ttgaggctct gctccacagc ggtttcaagc gcaggaacgg
     3541 ttgcacgggt gctttcgaga ttatagcgcg cctgctgaag agtcagttcg ccgatcaatc
     3601 cgctttcaaa ctgcgacttg agcagatcat aggttgcctg ctgaatttcg agattggaac
     3661 gggcgacccg caaccgctgc tgtccggcac gaagccgcac ataactctga gccgtttcgg
     3721 cggccagcga aacctgtacg tcagcctgac ccgcttcctg cgctttgagc gccgccagcg
     3781 aagcttcgac ggaacggcgg gttccgccga acagatcaat ttcccacgac gcatcgaatc
     3841 cgccgcgata cagactgctt tcgccggctt tcgaaatttc tttactggct ttctgccggg
     3901 tgtaactgcc gtttgcatcc agctgcggac cgtaggccga ccgtgcaatg ccgagctgag
     3961 cgcgggcggc gcggacagcg gcaacggcgg acttgaggtc gcagtttttc tgcaacgtct
     4021 catcgacgag cgccgtcagt tccggatcat tcagcgtcgt ccaccaggcc gcaggcagtt
     4081 ctgcgggctg tccgttgagc tgcggaagtt ccggcgcttt gtaatccggg ccgaccgaca
     4141 gacatccgcc gaagaggaac gggacaagca gaatcaccag caggtgcagt ttgcttttgc
     4201 tcacacggat ggcatggctc ttttcgcgca atgtctgaaa cagcacatag agcgcgggaa
     4261 tcaggatgat accgccgacg gttgcagcca gcatgcccca gaaaacggtc gtgccgattg
     4321 atttgcgcgc ggcggaaccg gcgccggtgg cgagtaccat cggaagcaca ccgagaataa
     4381 aggtgaaggc cgtcatcaga accgcccgga accgctgctt catgccgtcg gccgccgcat
     4441 cacgaatact tagaccttcc tcatgccggg ctctggaaaa ctcaacgatc agaatggcat
     4501 tcttactggc gagtccgacg agaagaatca gtccgagctg cgcatagatg ctcagataca
     4561 tatggttgaa cagcagcccg gccagcgcgc ccagactcgc gaccgccgtg gagagcatca
     4621 ccggcagcgg aatgatccag ctttcgtact gcgcgaccag aaacagaaag ccgaagatca
     4681 gcgccatggc gatgagcagc gtgcttccgc cggaggcttt ggcttcctga taactcagat
     4741 ccgtccagtc gaacgtgtag ccgggcggca aagtttcgcc ggccagtttg gtgacccgct
     4801 tcatggcttc gccgctgctg ataaacggaa gctgtacggc cgtgacggcc gcagcggtga
     4861 atttgttgta gcggtccacg gagcgcggcg cgagcgtgat gcgggacttg agcacactgc
     4921 ccagcggaac catgtcgccg cggttgtttt tgacataaat cttttcgaca tcctccacct
     4981 gattgcggaa cggccagtcg gcctgcacgt tgacgttgtt cacctgtcct tcaaagttca
     5041 cgttgttgac gtagcgtgat cccaggttgt cctgaagcgc cgcaaagatg gcgctggtat
     5101 ccacgcccat cagcgaggct ttgacacggt ccacatccat aaacaggtac ggagtattcg
     5161 ctgtataggt gctgaaggca tgcatcatgc ccggcatggt attgagcttc atgaggaagc
     5221 ccttcagcac ggcttccagt ttttgaggat cgttgtcgct gacggcctgc aggcggatgt
     5281 tcatcccgct gctggcaccc agcccgcgga tagccggcgg cgtgaacaga tagatctgcg
     5341 cgccgggaac ctgcgacagg cggccgagca gccgctgctg aatcgcggac gcctgcaaat
     5401 cgggctgctt ccctttattc cagaaacggg gagccttgcg ttcgctccag tctttcagac
     5461 cgcaaacgaa gaaggcgacg ttttcgccgg aaccgcccat caggctgaac ccggtgattt
     5521 gaatcacaaa agccacgccg ggctcgttgc gcagcggggt gatcgcctga gtgagcagcg
     5581 catcggtacg ggccagcgtc gcgccttccg gaagctggac ggcgccgaa
//
LOCUS       DAOPZO010000118         5552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_18364_length_5552_cov_11.045661, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000118 DAOPZO010000000
VERSION     DAOPZO010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18364_length_5552_cov_11.045661"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PKI68_07555"
     CDS             <1..972
                     /locus_tag="PKI68_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNX53584.1"
                     /translation="MNFQKRAKEIMDVEIAGMEKVRDSINGNFSTAIEWILATIKNGG
                     KVVVTGVGKNFHIGQKMVATFNSTGTPAALLHPIEAMHGDFGLVGEKDIVLALSYSGG
                     SDELLALLPALKRRGLKIIGMTGDETSPLGQQSDLVLPVTVDKEACPFGMAPTTSTTV
                     TLALGDALAIVLLEARGFKKEDYAKLHPGGAIGRTLLLKVSDVMRTKDRLAKVQSGAK
                     VKEAVLAMTGARSGCVAVVDATEKLLGIFTDGDLRRHLIETPDITEVSIDSVMTRNPI
                     TLKADQLAVDILKIYEEKNIDDLMVVDDAGHIVGAVDIQDLPKLKIL"
     gene            complement(992..1222)
                     /locus_tag="PKI68_07560"
     CDS             complement(992..1222)
                     /locus_tag="PKI68_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53585.1"
                     /translation="MPVDPVSLFTLPLALSLILLFAAAAAYDLITFRRRRKNGNKAVY
                     RCAACRHIYTAPHRTPLARCSKCGQQNEPVRE"
     gene            complement(1222..1902)
                     /locus_tag="PKI68_07565"
     CDS             complement(1222..1902)
                     /locus_tag="PKI68_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53586.1"
                     /translation="MKITVQLTPALTAALLCSAVWFSFWMLAFRPAVLRPPIPSVHPE
                     ATRLVTDDETLRKLKAPTLFALPSGEGFSGQFLENRVDLRLTLEKSSSPVRFLAPENI
                     AAPGVNRALLTSETDLPPGALPAPESAAPRTEPRLAEGTRLFLSPELKLRAAETLQMN
                     IAGKGLPETVRINLTVRPDGTVEHALFDSPATNAFLLSAVRQLRFKPAAEKTEGWIDI
                     RFAQEGKE"
     gene            complement(1899..2378)
                     /locus_tag="PKI68_07570"
     CDS             complement(1899..2378)
                     /locus_tag="PKI68_07570"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX53587.1"
                     /translation="MNRNDCEAVLHLHRTFLPRRRTGRGFLTGTVFLNAALLFSAFVL
                     VTSHFVLKPGILLDLPAASQTESIQFNDMVLSVSREGLFFFNDEQVQSAQLESVLHAA
                     AQERPGIALILEADASMPQATAATIYDAAARAGFRQVLIATRNPAPVTPAAPVAPAP"
     gene            complement(2368..2997)
                     /locus_tag="PKI68_07575"
     CDS             complement(2368..2997)
                     /locus_tag="PKI68_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNX53588.1"
                     /translation="MVGWINSGGPVMWLILAGGFIALTIFLERFFHLHRAQIKIDDFL
                     NGITTNLTHGNDLEAISICDQTPGPVAHLVRTALLHRDDSKDELIKAVQQAGIREIPR
                     LERHMNLLITLAQVLPMLGLLGTVFGLINILTALQSGSPLAEIGDLSSGLWSALLTTA
                     AGLSTGIPAYAGYHFLISRVESIALDMEQTAGEIIYFLTHPAAGDRHEP"
     gene            complement(3021..5504)
                     /locus_tag="PKI68_07580"
     CDS             complement(3021..5504)
                     /locus_tag="PKI68_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX53589.1"
                     /translation="MNFRLSRLSALLLLLAPALFAQEPASFPQIRAALADELYPFAEQ
                     QTWEALSTQRTPEEEATLTVLLIRALSGQQKFNDAVILADESAGLPKQDAFAYWRARA
                     LFEAGNFNAAIQKLGTLPKDSPYTPAALRLKGRAEQSSGDFKSAQKTFEAFREQFPSD
                     ENAAQNLLDLAGIYLERSRKSDATKALHELIGRFPDSTLADSARLVLARQLIASGGKD
                     EQSEASLLLNTLGARETAHPRLRSAAWVELSAIEQRAGNAAGAADALAKAEKLTGEAA
                     LRVRQKAVRAGLLADEKKTKDAFALFDEAVKEAPNTAVAAEVLIQKAEALLKTQQFAD
                     AEKAFQAGLDITADPAAQARAQAGKGWSLWEQKRYEEAAFAFENSALKCTVPDSCATA
                     LIKAGDARLAAAQYEKARENYRRAASAYPQHPLAAQAMYQCGVASLLAGQPDAARGFF
                     DRTEKEFPQSGFAPQAALQQAELLKREQKQDAALELYRRIAAQYTNAAVQASALHQQG
                     LVLYRLGRWDAALENFRTVSEKFPDAPEAPQAFYMRGFCRYMQGSTEEALTICQSFIE
                     KYPGSVWTPEVLFWLGEHYYNRGSYAQAKATFLDIAARFPKHELSDDALFWAGSSLLK
                     QDSFLEAFTVFSRLAKEYPDSPLILKTRFAQGETLTELGEFPRAILAYEEVIKTAPDD
                     PLADRARGRLADCLFTLGTSEAARYQEALDAYQSLYKRPAAPFALRLQALYKIARCEA
                     KLGLKEKAFAHYMEVVYSGAGQTEPLSPEAAPWFTRAAFDAAAWQEQQLQWKEAVNIY
                     ERIIQANVPAKSEAQKRIEKIQREHAPAF"
BASE COUNT         1068 a         1515 c         1815 g         1154 t
ORIGIN      
        1 atgaattttc agaagcgcgc caaagagatc atggatgttg aaattgcggg tatggaaaaa
       61 gtccgcgaca gcatcaacgg caatttcagc accgcaattg agtggattct tgcgacgatt
      121 aaaaacggcg gcaaggtggt tgtcaccggc gtcggtaaaa actttcatat cggccagaaa
      181 atggtcgcca cgttcaacag caccggcacg ccggccgcgc tgctgcaccc gattgaagcg
      241 atgcacggcg acttcggtct ggtcggcgaa aaagacattg tgctggcgct cagttacagc
      301 ggcggttccg atgagctgct tgctctgctt ccggcgctca aacggcgcgg cctgaagatt
      361 atcggcatga ccggtgacga aaccagcccg ctcggacagc agagcgatct ggttctgccg
      421 gttacggttg ataaggaggc ctgtccgttc ggcatggcgc cgaccaccag caccacggtg
      481 acactggcgc tcggcgacgc gctggcgatt gtactgctcg aagcgcgcgg gtttaaaaaa
      541 gaagattatg ccaagctcca tccgggcggc gcaatcgggc gcacgctgct gcttaaggtt
      601 tccgacgtaa tgcgcacgaa agaccgtctt gccaaggtgc agagcggtgc caaggtgaaa
      661 gaggcggtgc tggcgatgac cggcgcgcgt tccggctgtg tcgccgttgt ggacgccacg
      721 gaaaaactgc tcggcatttt caccgacggc gacctgcgcc gccatctgat tgaaacgccc
      781 gatatcacgg aagtttccat cgactccgtt atgacgcgca acccgattac gctgaaggcc
      841 gatcagcttg ccgtagatat tctgaaaatc tacgaagaga aaaacatcga cgacctgatg
      901 gtggtggatg acgccggcca tattgtcggc gctgttgata ttcaggacct gccgaagctc
      961 aagatcctct gaagcggtct gccgcctttc cttattccct gaccggttcg ttctgctgtc
     1021 cgcatttcga gcagcgggcg agcggcgtgc ggtggggcgc cgtgtagatg tgacggcagg
     1081 cggcgcagcg gtagacggct ttatttccgt ttttgcgccg gcggcggaag gtgatcaaat
     1141 cgtaagccgc cgccgctgcg aaaagcagga tcagtgacag cgcgagcgga agtgtaaaca
     1201 ggctgaccgg atcgacaggc attattcttt tccctcctgc gcaaagcgga tatcgatcca
     1261 gccttccgtt ttttccgctg ccggtttaaa gcgcagctgc cggacggcgc tgagcaggaa
     1321 ggcgttggtc gccgggctgt caaaaagcgc atgttcgacg gttccgtccg ggcggacagt
     1381 caggtttatg cggacggttt ccggcagtcc ttttccggcg atgttcatct gaagggtttc
     1441 ggctgcgcgt aatttaagtt ccggcgacag aaacagccgg gtgccttctg caagccgggg
     1501 ttctgttcgc ggcgcagcag actccggtgc cggcagagcg cccggaggaa gatctgtttc
     1561 ggacgttaga agagcccggt ttacgccggg tgcggcaatg ttttccggcg ccagaaagcg
     1621 gaccgggctg gaagattttt caagcgtcag gcgcaggtcg acccggtttt caaggaactg
     1681 tccgctgaaa ccttcaccgg aaggaagagc gaaaagtgtc ggagctttga gtttgcgcag
     1741 ggtttcgtcg tctgtaacca gacgggtggc ttcgggatgt acggaaggga tcggcgggcg
     1801 cagcaccgcc ggccggaaag cgagcatcca gaaagaaaac cagacggcgg agcagagaag
     1861 cgcagccgta agtgcaggtg taagctggac agtgattttc acggtgcggg agcaacgggt
     1921 gcagcgggtg tgaccggtgc ggggtttcgg gtggcaatga gtacctgacg gaatccggcg
     1981 cgggcggccg catcgtaaat ggttgcggcg gttgcctgcg gcatacttgc gtcggcttca
     2041 agaataagtg caatgccggg gcgttcctgc gcggcggcgt gcaggacgga ttcaagctgt
     2101 gcggactgca cctgctcgtc gttgaagaaa aagagccctt cgcgcgaaac gctcagcacc
     2161 atgtcgttga actgtatgct ctccgtctgc gaggctgcgg gcagatcaag gagaatgccc
     2221 ggcttgagga caaagtgcga tgtgaccagt acgaaggcgc tgaaaagaag cgcggcgttg
     2281 aggaaaaccg ttccggtcag aaacccgcga ccggtccggc ggcgcggcag gaaggtgcgg
     2341 tgcagatgaa gtacggcttc gcagtcgtta cggttcatgg cggtctccgg cggccgggtg
     2401 tgtgaggaag tagatgattt ctccggcagt ctgttccatg tcgagcgcga tggattcgac
     2461 gcggctgatg aggaaatgat aacccgcata agcgggaatg ccggtgctga gtccggcggc
     2521 cgtcgtgagc agcgcgctcc agagtccgct tgagaggtcg ccgatctcag ccagcggcga
     2581 accggactgc agggcggtga gtatattgat caggccgaac acggtgccga gcagtccgag
     2641 catcggcaga acctgcgcaa gggtgatgag caggttcatg tggcgttcaa ggcgcgggat
     2701 ttcgcggatg ccggcctgtt gaaccgcttt gatgagttcg tctttcgagt cgtcgcggtg
     2761 cagcagtgcg gtgcggacga gatgggcgac cgggccgggc gtctggtcgc aaatgctgat
     2821 tgcttcgaga tcattaccgt gcgtgaggtt ggtggtaata ccgttcagga agtcgtcgat
     2881 tttaatctgg gcgcggtgca gatgaaaaaa gcgctccaga aaaattgtca gcgcaataaa
     2941 tccgccggca agaatgagcc acatgaccgg gccgccgctg tttatccatc caaccataat
     3001 caactccttt gcttcgattc ctaaaatgcc ggggcgtgtt cgcgctgtat tttttcaatc
     3061 cgtttctgtg cctcgctttt cgcgggcaca ttggcctgaa taatccgttc ataaatatta
     3121 accgcctctt tccattgcag ctgctgttcc tgccaggcgg cggcgtcgaa ggcggcgcgc
     3181 gtgaaccagg gcgcggcttc gggcgaaagc ggttcggttt ggcccgcacc gctgtagacc
     3241 acttccatgt aatgggcaaa ggctttttct ttcaggccca gcttggcttc gcagcgggcg
     3301 attttataaa gcgcctgcag ccggagcgca aacggcgcgg cgggacgttt gtaaagcgac
     3361 tgatacgcat cgagagcttc ctggtagcgg gccgcttcgg aggttccgag cgtgaacagg
     3421 cagtcggcaa gacggccgcg ggcgcggtcg gccagcggat cgtccggcgc ggttttgatg
     3481 acttcttcgt aggcgagaat ggcgcgcgga aattcgccaa gctcggtcag ggtttcgccc
     3541 tgcgcgaaac gggttttcag tatcagcgga ctgtcgggat attctttggc gaggcggctg
     3601 aacaccgtga aggcttcgag aaagctgtcc tgtttaagaa gggagcttcc ggcccagaag
     3661 agagcgtcgt cggacagctc atgtttcgga aaccgcgcgg cgatgtcgag gaacgtggct
     3721 tttgcctgcg cgtaactgcc gcggttgtag taatgctcgc ccagccagaa aagcacttcc
     3781 ggcgtccaga cagagccggg atatttttcg ataaacgact gacagatggt cagagcttct
     3841 tctgtgctgc cctgcatata acggcagaat ccgcgcatgt aaaaagcctg cggagcttcg
     3901 ggggcgtccg gaaatttttc gctgacggtc cggaagtttt ccagtgcggc gtcccaccgt
     3961 ccgaggcggt aaagaacaag tccctgctgg tgcagcgcgg aggcctgtac ggcggcgttg
     4021 gtgtactgcg cggcaatccg gcggtacagc tccagcgccg catcctgctt ctgctcgcgt
     4081 ttcagcagtt cggcctgctg cagggcggcc tgcggggcaa atccgctctg cggaaactct
     4141 ttttctgtcc ggtcaaaaaa accgcgcgcg gcatcgggct gtccggcgag caggctggcg
     4201 acgccgcact gatacattgc ctgggcggca agcggatgct gcggataagc ggacgctgcg
     4261 cggcggtagt tttcgcgggc cttttcatac tgtgcggcgg caagccgcgc atcgcctgct
     4321 ttgatcagag cggttgcaca gctgtcgggt acggtgcatt tcagtgcgga gttttcaaaa
     4381 gcaaaggcgg cctcttcgta gcgcttctgt tcccaaaggc tccagccttt accggcctgt
     4441 gcgcgggcct gcgcggcggg atcagccgtg atgtcgagcc cggcctggaa ggccttttcg
     4501 gcgtcggcaa actgctgcgt tttcagcagt gcttccgcct tctggatcag cacctcggcg
     4561 gcgacggcgg tattcggtgc ttctttgacc gcttcatcga acagtgcaaa agcgtctttg
     4621 gttttcttct cgtcagccag cagtccggcg cggacggctt tctgacggac gcgcagcgcg
     4681 gcttcgccgg tcagcttttc ggccttggcg agcgcgtcgg ccgctccggc ggcgttaccg
     4741 gcgcgctgct caatggcgga aagttccacc catgcggcgg aacgcaggcg cgggtgcgct
     4801 gtttcgcgtg cgccgagtgt attgagcaga agagatgctt cgctttgttc gtccttaccg
     4861 ccagaggcga tcagctggcg cgccagcaca aggcgggcgc tgtcggccag tgtgctgtcc
     4921 gggaagcgtc cgatcagttc gtgcagagct ttggttgcgt cgctcttcct gctgcgttcc
     4981 agatagattc cggcgaggtc gagcaggttc tgcgcggcgt tttcgtcgga cggaaactgc
     5041 tcgcggaacg cttcaaaagt tttctgtgca gacttgaaat cgccggacga ctgctcggct
     5101 cgtcctttca gacgaagcgc ggcgggagta taggggctgt ctttcggcag agttccgagt
     5161 ttctggatgg ccgcgttgaa attaccggcc tcgaacagcg cacgggcccg ccagtaggcg
     5221 aaagcatcct gcttcggcag accggcggac tcgtcggcca gaatcactgc gtcgttaaat
     5281 ttctgctgac cggacaaggc ccggatcagc aggacggtca gcgttgcctc ctcttccggc
     5341 gtacgctgtg tgctgagcgc ttcccacgtc tgctgctcgg cgaacggata cagctcgtcc
     5401 gccagcgccg cccggatctg cggaaacgaa gccggctcct gcgcaaacag cgcaggcgca
     5461 agcagaagca ataaagcgga aagtctggaa agccggaaat tcattggcgc ggagtatcac
     5521 gtttcctgcc gccttcttcc agcctttttc ca
//
LOCUS       DAOPZO010000119         5548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_18379_length_5548_cov_9.615147, whole genome shotgun sequence.
ACCESSION   DAOPZO010000119 DAOPZO010000000
VERSION     DAOPZO010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18379_length_5548_cov_9.615147"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /gene="panB"
                     /locus_tag="PKI68_07585"
     CDS             complement(<1..755)
                     /gene="panB"
                     /locus_tag="PKI68_07585"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNX53590.1"
                     /translation="MKQTVAGIRKLKGERKIAVLTAYDWLSARYVDEAGIPVVLVGDS
                     LANTVLGYETTLPVTMDEMVHHTAAVSRGVKEALIVADMPFMSYQGTLEHALENAGRF
                     IKEGRADAVKIEGGAMRADLIEKLIANGVPVLGHLGLLPQSVKETGFKMQGRTEDAAR
                     KLMDDARALEQAGVFAIVLECVPAELAQKITAALTVPTIGIGAGPHCDGQVLVFTDVL
                     GLGHGHVPGFVKKFADLKPQIQNALAAYKKEVE"
     gene            complement(761..1270)
                     /gene="folK"
                     /locus_tag="PKI68_07590"
     CDS             complement(761..1270)
                     /gene="folK"
                     /locus_tag="PKI68_07590"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNX53591.1"
                     /translation="MTETGFCLGSNVGGRLRLLSQAKTLILLEPDVKFVDQSPAYETE
                     PVGVKPEHKAMKFLNAVLIVESPYTAEEWLPKIKKIEERLLRVRTEDRNAPRTIDIDI
                     LFCGDQTVDSDLLQVPHSRWTERRFVVQPLADIRPELILPGTKGAPVRKILARMPDKD
                     DVRLFSEKW"
     gene            complement(1267..1740)
                     /locus_tag="PKI68_07595"
     CDS             complement(1267..1740)
                     /locus_tag="PKI68_07595"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNX53592.1"
                     /translation="MSIKVGKPAPAFTLKGSDGEEHSLADYLGKTVVLYFYPKDDTPG
                     CTKEACGFGELRTKFKKLNAVVLGVSKDSLTSHSNFIKKFKLPFVLLSNPDASVMKLY
                     DAFGVKILYGKETTGTIRSTVIIGPDGTVAKHWKKVKKAEEHPAQVLEFLEAQAQ"
     gene            complement(1742..2557)
                     /gene="dapF"
                     /locus_tag="PKI68_07600"
     CDS             complement(1742..2557)
                     /gene="dapF"
                     /locus_tag="PKI68_07600"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNX53593.1"
                     /translation="MKIKFWKMHGAQNDFVLFDDRRGRFPAADRAFIARVAARRSGVG
                     SEGVILIQKSAAADFRMRFFNPDGGEVGMCGNGARCVARLAFELGAAGKKMTIETSAG
                     PIKAQVMQKGVRLWMTGPEGWKLGGLLELAGRNLAYDFVNTGVPHVVIRTAELSKVNV
                     CETGSAIRYHRDFAPAGTNVNFMDVSPDGELTVRTYERGVEAETLACGTGVTACGLIA
                     AKRGWITLPVSIHVAGGDVLTVDGRLTADGADGVTLTGPAEHVFQGTIEYGED"
     gene            2587..4653
                     /gene="ligA"
                     /locus_tag="PKI68_07605"
     CDS             2587..4653
                     /gene="ligA"
                     /locus_tag="PKI68_07605"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNX53594.1"
                     /translation="MSATLNSTEARKETEKLRAEIERHNRLYYVEASPQISDRDYDAL
                     LKRLETLEKQFPELAAPDSPTRRVGGEPLKGFENVRHSVPMISLANTYNKEELIEFDA
                     RVKKLLGGTPCSYVLEPKIDGVAISLRYENGLLVRAVTRGDGTTGDDVTANIRTIKSI
                     PLRLSNNPPDVLEVRGEVYMTRKGFAALNEERQEAGLEPFANPRNACAGSLKQLDPRI
                     VASRPLDALFYGLGECSAVYETHEQMLAALTGHGLRITPQFWVCGEIAEVLEKLDVLA
                     GMRHSFPFEMDGGVIKINQRSLYETLGSTAKSPRWAVAYKYEPEQAETLLKEITIQIG
                     RTGVLTPVAELEPVQLAGTVVKRATLHNEDEIKRKDIRVGDRVIVEKAGEIIPAVVRV
                     VTEKRTGRETPFDMQAACKALGINPVKNEGEVAWRIDDLHHPAMLKRWLTYFASRNGM
                     DIEGLGESVVEQLVDAGLVKSPADLYDLKKSQLLGLEGFKDKKAQNLIDGIAASKSRP
                     FDRILFALGIRHVGSGSARVLAQSFPDIEKLTAAPIETLEEIRDIGPVVGQSICDFFQ
                     TPSNRKLIERLQAAGVNFAQTAKAASDEFAGLTFVLTGTMESMSREEAGEQIRARGGS
                     VTSSVSKNTTYVVAGEKAGSKLTKAEELGVKVLSEQQFLKLLGNVEATPSSRLQKEKP
                     GQGELF"
     gene            4716..>5548
                     /locus_tag="PKI68_07610"
     CDS             4716..>5548
                     /locus_tag="PKI68_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNX53595.1"
                     /translation="MAKKMGLGRGLDALIQEKKVTAPVMDAPAPDSTGITKISVSRIK
                     ANPMQPRRIFRDEALNDLIASVKEHGILQPLLVRQKETHFELIAGERRLRAAKAANLP
                     EVPVILLDIGDQEALEIALIENLQRDDLNLMEEAEGYRELADKFNLTQEEIAKRVGKA
                     RASVANMLRLLDLPAGIKKMLEGGQISAGHGKVLLSVEIDAEKELLAQRILREGLSVR
                     ALEKIIEKLHKPAKKPRAEKSDLPIDYVRHLSEKLHQHFGTAVHITSTKTLSNGKKVK
                     GA"
BASE COUNT         1263 a         1818 c         1420 g         1047 t
ORIGIN      
        1 tcttcaacct cttttttata ggctgcgagc gcgttctgaa tctgcggctt caggtcggcg
       61 aattttttta cgaagccggg gacgtgcccg tggcccagac cgagaacatc ggtgaaaacc
      121 agcacctgtc cgtcgcagtg cggtcccgcg ccgatgccaa tggttggaac cgtcagcgcg
      181 gcagtaatct tctgcgccag ctccgccgga acgcattcca gcacgatggc gaacacgccg
      241 gcctgttcaa gcgccctggc gtcatccatc agcttgcgcg cggcgtcctc ggtccggccc
      301 tgcatcttga agccggtttc tttgacgctc tgcggaagca gaccgagatg cccaagtacc
      361 ggaacgccgt tggcgatcag cttttcaatc agatcagcgc gcatcgcgcc gccttcgatt
      421 ttgacggcat cggcgcggcc ttctttgata aagcgtccgg cgttttccag cgcgtgctcc
      481 agtgtaccct gataggacat gaacggcatg tcggcgacaa tcagcgcctc cttcacaccg
      541 cgcgaaacgg cggcagtgtg atgaaccatt tcatccatcg tcaccggcag cgtcgtttcg
      601 taaccgagca ccgtgttcgc cagcgaatcg ccgaccagta caaccggaat tcctgcctcg
      661 tccacatagc gcgcactcag ccagtcgtag gccgtcagca cagcaatctt ccgttcacct
      721 tttaattttc taataccggc aactgtctgc ttcatagaaa tcaccatttt tcagagaaaa
      781 gccgaacatc atccttatcg ggcatccgcg caagaatttt ccggaccggc gcccctttgg
      841 taccgggcag aatcagctcc ggacgaatgt ccgccagcgg ctgcacgacg aagcggcgtt
      901 ccgtccagcg cgaatgcgga acctgcagca ggtcgctgtc gaccgtctgg tcgccgcaaa
      961 aaagaatatc aatgtcgatc gtgcgcggcg cgttgcggtc ttccgtgcgc acccgcaaaa
     1021 ggcgctcctc gattttttta attttcggca gccattcctc cgcagtgtac gggctttcca
     1081 caatcagcac cgcgttcaga aatttcatgg ctttatgctc cggcttcacg ccgaccggct
     1141 ccgtttcgta agccggagac tgatcgacga acttcacgtc cggttccagc agaatcagcg
     1201 ttttcgcctg actcagcagc cgcaaccttc cgccgacatt tgatcccaga caaaacccgg
     1261 tttccgtcat tgcgcctgcg cctccagaaa ttccagcacc tgtgcgggat gctcttcggc
     1321 ctttttgact ttcttccaat gcttggcaac cgttccgtcc gggccgatga tcacagtcga
     1381 ccggatcgtt ccggtggttt ctttgccgta cagaatcttc accccgaacg catcatagag
     1441 tttcatcacg ctggcgtccg gattggaaag cagcacgaac ggcagtttga attttttgat
     1501 gaaattactg tgcgacgtca gactgtcttt actgacgccc agcaccactg cgttgagctt
     1561 cttgaatttg gtccgcagct cgccgaaacc gcacgcttct ttggtgcagc cgggggtgtc
     1621 atctttcgga taaaaataga gcaccactgt ttttccgaga taatcggcaa gcgaatgctc
     1681 ctcgccgtca ctgcccttca gcgtaaatgc cggggccggt ttaccaactt taatcgacat
     1741 cttaatcctc tccgtattca attgttcctt gaaaaacatg ttcggccggg cctgtcagcg
     1801 tcacgccgtc cgcgccgtcc gctgtcagcc gtccatccac ggtcagcaca tcgccgcccg
     1861 ccacgtgtat gctcaccggc agcgtaatcc agccgcgctt tgccgcaatc agcccgcagg
     1921 ccgtcacgcc ggttccgcag gcaagagttt cggcttccac gccgcgttca tacgtgcgca
     1981 cagtcagctc tccgtccggc gaaacatcca taaaattcac attcgtgcct gccggagcaa
     2041 aatcgcggtg ataccgaatt gctgaaccgg tttcgcacac attcactttc gacagctcag
     2101 ccgtacgtat gaccacatgc ggcaccccgg tgttcacaaa atcatatgcc agattccggc
     2161 ccgccagctc cagcagtccg cccagcttcc agccctccgg cccggtcatc cagagcctca
     2221 cccctttttg catcacctgc gctttaatcg gtccggcaga agtctcaatc gtcatttttt
     2281 ttccggccgc gcccagctca aacgccagcc gcgcaacaca gcgcgcgccg ttgccgcaca
     2341 tcccgacctc gccgccgtcc ggattaaaaa accgcatccg gaaatccgcc gccgccgact
     2401 tctgaatcag aatgacacct tcagatccga cgccggaacg gcgggccgcc acgcgggcaa
     2461 taaacgcccg gtcggcggca ggaaacctgc cgcgccggtc gtcgaaaagc acgaaatcat
     2521 tctgcgctcc gtgcatcttc cagaatttaa ttttcatgga aaaagtcccg tgatagcttc
     2581 tgcccgatga gcgccacatt aaacagcacc gaagcacgga aagaaacaga aaaactgcgt
     2641 gccgagatcg agcgccacaa ccgcctttac tacgtcgaag cctctccgca aatctccgac
     2701 cgcgactatg acgcgcttct caaacggctc gaaacactag aaaaacagtt tcccgaactc
     2761 gccgcacccg acagtccgac ccgccgcgtc ggcggcgaac cgctcaaagg ctttgaaaac
     2821 gtccggcaca gcgtgcccat gatcagcctc gccaacacct acaacaaaga agagctgatc
     2881 gaattcgacg cccgcgtcaa aaagctgctc ggcggaacac cctgttcgta cgttctcgaa
     2941 ccgaaaatcg acggcgtcgc catttcgctg cgctacgaaa acggactgct cgtccgcgct
     3001 gtcacgcgcg gcgacggcac caccggcgac gacgtcaccg ccaacatccg caccatcaaa
     3061 tccattccgc tccggctttc aaacaatcct cctgacgtac tggaagtgcg cggcgaagtt
     3121 tacatgaccc gcaaaggctt cgccgcgctc aacgaagaac ggcaggaagc cgggctcgaa
     3181 ccgttcgcca atccgcgcaa cgcctgcgcc ggctcgctca aacagctcga cccgcgcatc
     3241 gtcgcttcgc gcccgctcga cgccctcttc tacggactcg gcgaatgttc cgccgtttac
     3301 gaaactcacg aacagatgct cgccgccctg accggtcatg gattacgcat caccccgcaa
     3361 ttctgggttt gcggcgaaat tgctgaagtt ctcgaaaagc tggacgttct cgccggcatg
     3421 agacacagct tcccgtttga gatggacggc ggcgtcatca aaatcaacca gcgcagcctt
     3481 tacgaaacgc tcggctccac cgccaaaagt ccgcgctggg ccgtcgccta taaatacgaa
     3541 ccggagcagg ccgaaacgct gctcaaagaa atcaccattc agatcggccg gaccggcgtg
     3601 ctgacgcccg tcgccgaact cgaaccggtt cagctggccg gaaccgtcgt taaacgcgcc
     3661 acgctccaca acgaagacga aatcaaacgc aaagacatcc gcgtcggcga ccgcgtcatc
     3721 gtcgaaaaag ccggcgaaat tattccggcc gtcgtgcgcg tcgtcaccga aaagcgcacc
     3781 ggccgggaaa cgccgttcga tatgcaggcc gcctgcaaag ccctcggcat caatccggtc
     3841 aaaaacgaag gcgaagtcgc ctggcgcatc gacgatctgc accatccggc gatgctcaag
     3901 cgctggctca cctacttcgc ttcgcgcaac ggcatggata tcgaaggcct cggcgaatcg
     3961 gtcgtcgaac agctcgtcga cgccgggctg gtgaaaagtc ctgccgacct ttacgacctg
     4021 aaaaaaagcc agctgctcgg cctcgaagga ttcaaagaca agaaggcgca gaacctgatc
     4081 gacggaatcg cggccagcaa aagccgcccg ttcgaccgga ttctattcgc gctcggcatc
     4141 cgccacgtcg gcagcggctc ggcgcgggtt ctcgcgcaga gttttccgga cattgaaaaa
     4201 ctgacggctg ctccgattga aacgctggag gaaatccgcg acatcggccc ggtcgtcggc
     4261 caaagcatct gcgacttctt ccagactccg tccaaccgga aactgattga gcggctgcag
     4321 gccgcaggcg tcaacttcgc gcaaaccgcc aaagccgcca gcgacgagtt cgccggactg
     4381 accttcgtcc tgaccggaac catggaaagt atgagccgcg aagaggccgg cgaacagatt
     4441 cgcgcccgcg gaggttccgt cacctccagc gtttccaaaa acaccaccta tgtcgtcgcc
     4501 ggagaaaaag ccggctccaa gctgaccaaa gccgaagaac tcggcgttaa agttttatct
     4561 gaacagcagt ttttgaagct gctcggcaac gtagaggcga cgccctcgtc gcgtctgcaa
     4621 aaagaaaagc ccggacaggg cgagctgttc tgaacaaaaa ctttcctatt cgtgcttatt
     4681 cgtgttattc gtgggcactt caactgaggt aaattatggc aaaaaaaatg ggtttaggtc
     4741 gcgggctcga tgcgctcatt caggaaaaaa aggtgacggc tccggtcatg gacgccccgg
     4801 cgccggacag caccggcatc actaaaattt ccgtcagccg gatcaaagcc aacccgatgc
     4861 agccgcgccg catcttccgc gacgaagcgc tcaacgacct gatcgcctcg gtcaaagaac
     4921 acggcatcct ccagccgctg ctcgtccggc agaaagaaac tcattttgaa ctgatcgccg
     4981 gggagcgccg cctgcgcgcc gccaaagccg ccaacctgcc ggaagtaccg gtcatcctgc
     5041 tcgacatcgg cgaccaggaa gcgctcgaaa tcgcgctgat cgaaaactta cagcgcgacg
     5101 acctgaacct gatggaagaa gccgaaggct accgcgaact ggcggacaaa tttaatctga
     5161 cgcaggaaga aatcgccaag cgcgtcggca aagcccgcgc cagcgtcgcc aacatgctcc
     5221 gcctgctcga cctgcccgca ggcatcaaaa aaatgctgga aggcggacag atttccgccg
     5281 ggcacggcaa agtgctgctc tcggttgaaa tcgacgcgga aaaagaactg ctcgctcagc
     5341 ggattctgcg cgaaggcctc tccgtccgtg cgctcgaaaa aatcatcgag aagctgcaca
     5401 aaccggccaa aaagccgcgc gctgaaaaga gcgacctgcc gatcgactac gtccgccacc
     5461 tctccgaaaa actgcaccag cacttcggaa ccgccgtaca catcacctcg accaaaacgc
     5521 tctccaacgg caaaaaggtt aaaggcgc
//
LOCUS       DAOPZO010000120         5506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_18569_length_5506_cov_8.863878, whole genome shotgun sequence.
ACCESSION   DAOPZO010000120 DAOPZO010000000
VERSION     DAOPZO010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18569_length_5506_cov_8.863878"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="PKI68_07615"
     CDS             complement(<1..1339)
                     /locus_tag="PKI68_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53596.1"
                     /translation="MIRPVEHPETLLATERFFAPGGGLEQAHAGNDFPFEIRPQQQQM
                     ARAVAEACANSCHLAVEAGTGVGKSFAYLVPLILNALAQNVRAVVATYTITLQEQLME
                     KDIPFLQKALGVDFKARLVKGRSNYLCLRRLARARQMGGDLFDTAKEIELGRIRAWAD
                     RTTDGSRQELEPQPSVDVWSAVCVEHGNCLGKKCPDYEHCFVMKARAGLHEANLLVTN
                     HHLFFSELAVRSEGGAFLPDYGMVVFDEAHQMENVASEHLGIRLSRYAMEHWLRRLFT
                     QDNRKGLFAVLRDGKGADLTNRIWDESERFFKAMHKCCKLSETSQTVRLREPPAVETR
                     LPERLAELDAHIGGIIRDMEDESLKAELQSARMKGKAFIEMLTTFLKQSEPGHVYWAE
                     LEGRTHKQPVLYSAPVDVAHLLKGMLFDEVPSVVMTSATLAVGEDLSWFRKRIG"
     gene            1481..1792
                     /locus_tag="PKI68_07620"
     CDS             1481..1792
                     /locus_tag="PKI68_07620"
                     /inference="COORDINATES: protein motif:HMM:NF023184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HNX53597.1"
                     /translation="MNKKQKQKTVEVMIRYFCKAKHRERDLCNSCSGLLQDITVRIDR
                     CHLDEKISSCFMCPDYCYSPEMRTKIKAAMRFSAPRMLFRHPVLIFKHLRHLQKLHRH
                     L"
     gene            complement(1921..2631)
                     /locus_tag="PKI68_07625"
     CDS             complement(1921..2631)
                     /locus_tag="PKI68_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNX53598.1"
                     /translation="MNMEVTILCGTAATLGFIHTVLGPDHYLPFVVMARARNWSASKT
                     AWITFACGLGHVLSSVVLGLIGIAFGIAVMKLEAFESFRGNLAAWALIGFGFAYMGWG
                     IQRGVRNRPHTHLHLKDRGVPAHSHTHDGEHFHTHEPAKKANITPWILFTIFVLGPCE
                     PLIPLLMYPAAQHSLSGTVLVATVFSAVTISTMLTIVMAASWGISFARLGKLERWTHA
                     LAGGAICLSGLAIQFLGL"
     gene            2971..3453
                     /locus_tag="PKI68_07630"
     CDS             2971..3453
                     /locus_tag="PKI68_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53599.1"
                     /translation="MKERKFPFIPKSSKGLEIGDFWSIPLDGGRFACGRVIGMCPVGW
                     RASQRGFFAGLLDWVSDSPPTAAGIAGVRTLIQGGAHIKTILATGGTILGNRSLEEDG
                     IMPQLFIEFPDNRTAYVFRGMEQLRVASQEEIRSLPKLSTFGFMYMRTYANTHLHRRA
                     "
     gene            complement(3522..3926)
                     /gene="nikR"
                     /locus_tag="PKI68_07635"
     CDS             complement(3522..3926)
                     /gene="nikR"
                     /locus_tag="PKI68_07635"
                     /inference="COORDINATES: protein motif:HMM:NF003381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HNX53600.1"
                     /translation="MERFSISMEDDLVQKFDGHLERRGYSNRSEAVRDLVRKALIDEE
                     WQAGEEVVGVITLVYDHHQRQLQDKLTAIQHDHHDRIISSTHVHMDHHNCLEVIIVKG
                     EAREIREMADQLKAAKGVKSGELTAASTGRKL"
     gene            complement(4046..>5506)
                     /gene="lysS"
                     /locus_tag="PKI68_07640"
     CDS             complement(4046..>5506)
                     /gene="lysS"
                     /locus_tag="PKI68_07640"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNX53601.1"
                     /translation="FMQDNEYRRQRIENMKQLEAAGYPAYGSAFKRTDTLAGLTASFE
                     EGKTVSACGRIVAMRKMGKMAFAHLSDGSAKFQLMVKKDLIGEEIFAAFKLLDLGDII
                     GVTGELFITQTGEKTVRIIEWKLLCKSLIPLPEKFHGLQDVETRYRQRELDLISNEEV
                     MDLFKKRTAVLSEIRAFLAARSYNEVETPMIQQIAGGAAASPFKTRYNALGTDMFFRI
                     APELYLKRLIVGGMDKVFEMNRNFRNEGLDRTHNPEFTALEIYEAYGDMRSMQELVQS
                     LVTHLSQKIFGRLEVEWMGNTINLTTPWKEIAYRDLIKERLGADWFEKSVDQARAKAD
                     ELGIFVEPGAGFAAVTHEIYEKSIEGTLIQPTFVTRLPAYLVPLANPCADDPSLVDVF
                     ELIVGGKELAPAYSELNDALEQRRRFEEQAAGDPSKIDEDFLDALETGMPPTGGMGIG
                     IDRLMMLLTGSDAIRDVILFPQLKPRVQQVKTDLQK"
BASE COUNT         1239 a         1567 c         1481 g         1219 t
ORIGIN      
        1 cgccgatgcg cttgcggaac cagctcaggt cttcgccgac cgccagcgtc gcgctcgtca
       61 tcaccacgct cggcacttca tcaaacagca ttcccttcag cagatgagcc acatcgaccg
      121 gcgcagaata aagcaccggc tgtttatgcg tccgtccttc cagctccgcc caatagacat
      181 ggccgggttc gctctgtttc aggaacgtcg tcagcatttc gataaaagcc ttccccttca
      241 tccgtgcaga ctgaagctcg gccttgaggc tttcatcctc catgtcacgg atgatgccgc
      301 caatgtgtgc atcgagttcc gccagccgct ccggcaggcg ggtttcgacg gcaggcggct
      361 cgcgcagccg taccgtctgg ctggtttccg acagcttgca gcatttatgc atcgccttga
      421 aaaagcgctc gctctcgtcc cagatacggt tcgtgaggtc ggcgcccttc ccgtcgcgaa
      481 gaaccgcgaa aagacccttt cggttgtcct gcgtaaacag ccggcgaagc cagtgctcca
      541 tggcgtaacg cgacaggcgg attcccagat gttccgaagc gacattttcc atctggtgcg
      601 cctcgtcaaa aacgaccatg ccgtagtccg gcaggaacgc gccgccctcc gagcgcaccg
      661 ccagttcaga gaaaaaaaga tgatggtttg tcaccagcag attcgcctca tgaagtccag
      721 cccgcgcctt catcacaaaa caatgttcat agtctggaca ctttttgccg aggcagttgc
      781 cgtgctccac gcagaccgcg ctccagacat ccaccgaagg ctgaggctcc agttcctggc
      841 ggcttccgtc cgtcgtgcgg tcagcccagg cgcgaatccg ccccagctcg atttccttcg
      901 ccgtatcgaa cagatcgccg cccatctgcc gggcgcgcgc cagacgtctc aaacagagat
      961 aattggaacg tcccttcacc agccgggcct tgaaatcgac gccgagagcc ttctgcagaa
     1021 aaggaatatc cttttccatc agctgctcct gaagcgtaat cgtataggtt gccaccaccg
     1081 cgcggacgtt ctgcgccagc gcattcagga taagcggaac gagataggca aagctcttgc
     1141 cgacgccggt tccggcttcg accgcaaggt ggcacgaatt ggcgcaggct tccgccaccg
     1201 cgcgtgccat ctgctgctgc tggggacgga tttcaaacgg aaaatcgttg ccggcatgag
     1261 cctgctccag tccgccgccg ggcgcaaaaa agcgctcggt cgccagcagc gtttcgggat
     1321 gttcaacagg acgaatcatt ccgccacagc gtaccggaaa attccggagt ttggaaccgg
     1381 cattgccgct taattcggcg gatcaaaaac atcaaaaaac cggcggattt accttctttt
     1441 tcggacggct ttcattaaat tcacaaagat ttttagcagt atgaataaga agcagaaaca
     1501 aaaaacggta gaagtaatga ttcgttactt ctgcaaagca aaacaccggg agcgcgacct
     1561 gtgcaactcc tgctccggac tgcttcagga catcacagta cgtatcgacc ggtgtcatct
     1621 ggacgaaaaa atatcatcct gcttcatgtg tccggattat tgctacagcc ccgaaatgag
     1681 aacaaagatc aaagcggcta tgcgtttttc tgcgccgcgc atgctgtttc gccacccggt
     1741 tctgatattt aaacatctgc gccatctgca gaaactgcac cggcaccttt gagccgttat
     1801 gattgtagga cacgcttccc gtattcggga gcgtgtgatg atacaaaaac acaggctccc
     1861 cggaatacca aggaagcctg tcctacaaaa agacgatcgg caactgagtc gtcaaactct
     1921 ctaaagacca agaaactgga tcgcgagacc ggaaagacag atcgcaccgc ccgccagcgc
     1981 atgtgtccag cgctccagct tgccgagccg ggcaaagctg attccccacg aggcggccat
     2041 aacaatcgtc agcattgtac tgatcgtcac cgcactgaac accgtcgcca ccagcacggt
     2101 gccggaaagg ctgtgctgcg ccgccggata catcagcagc ggaatcagcg gctcgcacgg
     2161 accgagcacg aaaatggtaa aaagaatcca cggcgtaatg ttggcttttt tcgccggctc
     2221 atgcgtgtgg aaatgctcgc cgtcatgcgt atgcgaatgc gccggaacac cgcggtcttt
     2281 gagatgcaga tgagtatgcg gacggttgcg gacgccgcgc tgaatgcccc accccatata
     2341 ggcgaatcca aaaccgatca gcgcccatgc ggccagattg ccgcgaaacg attcgaaagc
     2401 ttccagcttc ataacggcaa tgccaaaagc aataccgatc aggccgagca ccacggaact
     2461 gagaacatgc cccagtccgc aggcaaaagt gatccacgct gttttggatg cactccagtt
     2521 gcgggcgcgg gccatcacaa caaacggcag atagtgatcc ggtccgagaa ccgtatgaat
     2581 aaaacccagt gtcgcagcgg ttccacaaag aatggtaact tccatattca taaaggcgct
     2641 cctgcaggtg attacgaatc gctaaaaagt aacaccggca aagcaaataa tccatcccga
     2701 aaactgacta aacccacccc gccgttccgg cacccctccg ccggagggga ttttaaacgc
     2761 tcccctccgt tggaggggtg gccaaaggcc gggtgggtgc aacatttgga ttgtgccatg
     2821 cgttgtttgc ctgcgttata gaaactaaaa acttacacct tgaccgctta tcagctcccc
     2881 ttacaatccg gctcgttggg tcgctttacg aaaatgtata aagcgtagta aacgttcgtt
     2941 caataacact gttaggccac agcacactgc atgaaagagc gtaaatttcc attcattcca
     3001 aagtcctcga aaggtttgga gattggcgac ttttggagca ttcctctcga cggcgggcgc
     3061 tttgcctgcg gtcgggttat aggcatgtgt ccagttggct ggagagccag tcagcgcggt
     3121 tttttcgcgg gactgcttga ttgggtttct gattcgccgc cgacggcggc aggtattgct
     3181 ggtgtgcgaa cgcttatcca aggtggagcg cacatcaaaa cgattcttgc caccggtggt
     3241 acgattctcg gcaatcgttc gctagaggag gatggcatca tgccacaact gtttattgag
     3301 tttcccgata atcgaacagc atatgtgttt cgcggcatgg agcagcttcg agtcgcttct
     3361 caggaggaga ttcgcagctt gccgaagttg agcacatttg gcttcatgta tatgcgcacg
     3421 tatgcgaata cacacttgca cagaagggcc taaaaattcg ctggagcgac cgcggcttcg
     3481 ccgcatcgct cagcttggcc gtcaatccgc aataaccgat tttacagctt ccgtccggtg
     3541 ctggcggcgg tgagttcgcc ggatttgacg cccttggccg ctttgagctg gtcggccatt
     3601 tcgcggattt cgcgagcctc gcctttgacg ataatcactt cgaggcagtt gtgatgatcc
     3661 atgtgaacgt gcgtcgatga aatgatccgg tcgtgatggt cgtgctggat ggcagtgagt
     3721 ttatcctgaa gctggcgctg gtggtggtca tacaccagcg taatcacgcc gacgacttct
     3781 tcgcccgcct gccactcttc atcaatcagc gctttacgca ccagatcgcg caccgcttcg
     3841 gagcggttgg aatagccgcg ccgttccaga tgtccgtcga acttctgaac cagatcgtct
     3901 tccatcgaaa tcgaaaagcg ttccatttca acactcctta tatcacagca atctaaagaa
     3961 tatttttaca gaaggaaacg aagggaaccc aaggtgggaa ttgaatagta gaacccggct
     4021 ttctgattcc tttgttatcc ttcgtttact tctgtaaatc agtttttacc tgctgcacac
     4081 gcggcttgag ctgcgggaaa agaatgacgt cgcggatggc gtcggagccg gtcagaagca
     4141 tcatcagccg gtcaatgccg atacccatac cgccggtcgg cggcataccg gtttcgagcg
     4201 cgtcgagaaa gtcctcgtcg attttcgacg gatcgcccgc agcctgttct tcaaagcggc
     4261 ggcgctgttc gagcgcgtcg ttgagttcgc tgtaagccgg cgccagttct ttaccgccga
     4321 caatcagttc aaaaacatcc accagcgacg gatcgtcggc gcacggattg gccagcggaa
     4381 cgagataggc cggaagccgg gtgacaaacg tcggctgaat cagcgtgcct tcgatgctct
     4441 tttcatagat ttcgtgggta acggcggcaa agccggcacc cggctcaaca aaaataccga
     4501 gttcgtcggc tttggcgcgc gcctgatcga ccgatttttc aaaccagtcg gcgccgaggc
     4561 gctctttgat caggtcgcgg taggcgattt ccttccacgg cgtggtcaga ttgatcgtat
     4621 tgcccatcca ttcgacttcc agccggccga aaattttctg cgacagatgg gtgacgaggc
     4681 tctgcaccag ctcctgcatc gaacgcatat cgccgtaggc ttcgtaaatt tccagcgccg
     4741 tgaattccgg attatgcgtc cggtcgagcc cttcgttgcg gaagttgcgg ttcatctcga
     4801 acactttatc catgccgccg acaatcagcc gcttgagata aagttccggc gcgatgcgga
     4861 aaaacatgtc ggtgccgagg gcgttgtagc gcgtcttgaa cggcgaagcg gctgcgccgc
     4921 cggcgatctg ctgaatcatc ggcgtttcga cttcgttata gctgcgcgcc gcgaggaaag
     4981 cgcggatttc gctgagtact gctgtgcgtt ttttgaagag atccatcacc tcttcattgg
     5041 aaatcaggtc gagttcgcgc tggcggtagc gggtttcgac atcctgcagt ccgtggaatt
     5101 tttccggcag cgggatgagc gatttacaca ggagcttcca ttcgatgatg cggacggttt
     5161 tttcaccggt ctgggtgatg aacagttcgc cggtcacgcc gatgatatcg ccgaggtcga
     5221 gaagcttgaa cgcggcaaat atttcttcgc cgatcagatc cttcttaacc atcagctgga
     5281 acttggccga accgtcagaa aggtgcgcga aagccatttt tcccattttg cgcatggcga
     5341 caatacgacc gcaggcgctg acggttttgc cctcttcgaa cgaggcggtc agtccggcca
     5401 gcgtgtcggt gcgtttgaag gcggaaccgt aggcgggata gcctgcggct tcgagctgct
     5461 tcatgttttc aatgcgctga cggcggtatt cattgtcctg cataaa
//
LOCUS       DAOPZO010000121         5489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_18645_length_5489_cov_8.567906, whole genome shotgun sequence.
ACCESSION   DAOPZO010000121 DAOPZO010000000
VERSION     DAOPZO010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18645_length_5489_cov_8.567906"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..2032
                     /locus_tag="PKI68_07645"
     CDS             302..2032
                     /locus_tag="PKI68_07645"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HNX53602.1"
                     /translation="MMKRGLFFGAALCVIVSTAAAKEPAAVLEQSSTPEPAGRIDQLV
                     FSRLEKIKIQPALCSDEVFVRRVYLDVIGKLPSAKEVVAFLDKPDAETRRSELIDELL
                     ARDEFADYWAMKWSDVLRIKAEFPINLWPNAAQAYHRWVRTSIAENKPYDRFVREMLT
                     ASGSNFRVGPVNFYRAMQSKTPRGIAATTALTFMGVRAESWPENRLDSMALFFSQVGY
                     KATSEWKEEFVFWDPLGTAADKANSAPGKDLITTANTGAVAQVSATPCGPVETAFPDG
                     KKILIPPDRDPREVFADWLITPENPWFSRCIVNRVWAWLLGRGIIHEPDDIRDDNPPS
                     NPELLAWLEKEFSAHQYDLKYLYRLILNSKTYQLSSVSKSSDPSAAAQFASYPLRRLE
                     AEVLIDAINSITGSSDLYTSAIPEPFTYIPKDQSAVSIADGSITSPFLALFGRSARAT
                     GMWNERDNEATPSQRLHLLNSGQIQEKLEKGPKLKALISAKKPPEEIIEDLYLTILSR
                     RPTKEEKQIVFAYGNLGTPENPPPPGKPGKTGNEKQKQQRKPGDPGRQSSWIDIAWAL
                     INSPEFLYRH"
     gene            2042..3349
                     /locus_tag="PKI68_07650"
     CDS             2042..3349
                     /locus_tag="PKI68_07650"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNX53603.1"
                     /translation="MNMTRRNALKYGLCGAGSALLANKLQAAAPLSTLPVKAKSVIQI
                     FLWGGMSHNDTWDPKPDSGRDYMGDFMKVLPTNVAGIQIGQLFPELAKQADKFSLIRS
                     MTHGNNGHETAAYYMQTGHSSNERITYPSIGAVFSYFKNPGYKGLIPPYVVMTRPQGR
                     FAEEGFLGPMYKPFATGGDPNAARFEVEGVIAKGISDERQAARRELLRKTDLLGQAMN
                     SAVLKDARIAEDRAYELILGSGKEVFNLSNEDDKLRDRYGRNTFGQECLAARRMVETG
                     VPYIVINYPGGWDTHKGHFARMRLQCPNLDQGLAALLEDLANRGLLDSTLVWCCGEFG
                     RGPKVDWQPPWNGGRNHYGAVFSVLVAGGGFRGGAIVGSSDEKAEAVSERPVYPVDLL
                     GSIYRLGGIDPAAMLPHPLGLEARVLPEISGTIKSGGLLTEIM"
     gene            3387..>5489
                     /locus_tag="PKI68_07655"
     CDS             3387..>5489
                     /locus_tag="PKI68_07655"
                     /inference="COORDINATES: protein motif:HMM:NF016071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HNX53604.1"
                     /translation="MKKLLIAAISGLLALQANEAAAQTAKKRIYIGYVYPAGGRQGTT
                     FPARVGGQQIEDACGATVSGEGVHVNLGTVFRKFDTQSSRLISEQLKLIKKEKSDLDE
                     TTLKIKEQIELLMSEAVKRAASESLATVITLDITVDADAAPGPREIRIMTPGGISNPL
                     PFYVGVLPENSLPPMKTSSFQILGKEQLALRKRPEEDAEKSITLPCTVNGQIASGEIN
                     RYRFSADKGDRLVISTLARQLIPYIADAVPGWFQPVLSLRNAAGTEIAYNDDYRFKPD
                     PVILCEIPEKGEYILSITDAIYRGREDFVYRITIGQTPFITGIFPPGARAGETVSADM
                     SGWNLDNAKLILPGPDAGESLHPAGAARHDVLSNLIPFAIDTLPECTERRSNNDLNTA
                     QDVTLPVIINGRIETPGDRDVFRFTAKKGDTVVAEVNARRLDSPLDSLLTLTGSDGKI
                     IAINDDHSDPGSGLNTHHADSYIMTELPADGTYYVQLTDTAHQGGSNNTYRLRISPPK
                     PDFALRTEPSHFDIRGNTASVTVYAIRKDGYNGPITLRLAEPDAGFDAKPVTLPAGAE
                     KIKFNIRCTAKDLKGSVPAVITGTAEIGGRQTIRQAVPAEDWMQAFLWRHLVPAQELV
                     IYRYDPSMSPDTRRPLPEDPDVSLYLKSNNSLTPEQKKIAGMIKQTASLYEKWLLTDS
                     FYQETIKGYLEYTTGKKPE"
BASE COUNT         1294 a         1733 c         1445 g         1017 t
ORIGIN      
        1 aaagaccaat ctccgatgac tgattaggtt taatcgtaca cccttaccga ctcgtaaggc
       61 catcccctac aaaatgatac gacaaaacac ctcggccgga gaccgatacc aagtaacgcc
      121 tgtttttaaa ccgcagaatt gtttttctcc gtttgtcaga gtctctattc cggcatagaa
      181 taagaagtga gcaacaaaaa ccgcagtttt tcgttctctt tataggcgtc tttttatccg
      241 gcccggaata tacttatgga gtttttgccg gcggaactct tttacgggag acggaaacaa
      301 catgatgaaa cgtggcttgt tttttggagc cgccctttgc gtcatcgtaa gtactgctgc
      361 ggcaaaagag ccggcagcgg tactggaaca gtcatccaca ccggaacccg ccggccggat
      421 tgatcagctg gttttcagcc ggctcgaaaa gattaaaatt caaccggcac tttgttccga
      481 cgaggtcttt gtccgccgtg tctatctgga tgtgatcggc aaactgcctt ccgccaaaga
      541 ggtcgttgca tttctcgata aacctgatgc ggaaaccaga cggagcgaac tgattgacga
      601 actgctggcg cgggatgagt ttgccgacta ctgggcaatg aagtggagcg atgtgctgcg
      661 catcaaggcc gagtttccga tcaatctgtg gccgaatgca gctcaggctt atcaccgctg
      721 ggtgcgcaca tcgattgccg aaaacaaacc ctacgaccgc tttgtccgcg aaatgcttac
      781 tgccagcggc agcaacttcc gggtcggacc cgtgaatttt taccgtgcga tgcagagtaa
      841 aaccccaagg ggaattgccg cgacaacggc gctgaccttt atgggtgtcc gcgccgagtc
      901 gtggccggaa aatcggctgg acagcatggc gctcttcttt tcgcaggtag gttataaagc
      961 aaccagcgag tggaaagaag agttcgtctt ctgggatcct ctcggaaccg ctgcggacaa
     1021 agccaacagc gctcccggca aggatctgat cactacggca aacaccggag ccgtcgcaca
     1081 ggtctccgca acgccctgcg gccccgtcga gactgcattc cccgacggca aaaagatcct
     1141 gatcccgccg gaccgcgacc cgcgcgaggt gtttgccgac tggctgatta cccccgaaaa
     1201 tccctggttc agccgctgca tcgtaaaccg cgtctgggcc tggctgctcg gccgcggcat
     1261 catccacgag cccgatgaca tccgtgatga taatccgcca tccaaccccg agctgctggc
     1321 ctggctggaa aaagaatttt ccgcacatca gtacgacctg aagtatctct accgcctgat
     1381 cctgaactca aaaacctacc agctctcttc ggtctccaag agcagcgatc cgtccgccgc
     1441 cgcgcagttc gcttcgtacc cgctccgccg tctggaagca gaggttctga ttgacgcgat
     1501 caacagcatc accggcagtt ccgatctgta caccagcgcc atccccgaac ccttcaccta
     1561 cattccgaag gatcagtccg ccgtctccat cgccgacggc agcatcacca gcccgttcct
     1621 cgcgctcttc ggccgctccg cgcgcgccac cggcatgtgg aacgaacgcg acaacgaggc
     1681 cactccgtcc cagcgtctcc atctgctgaa ttcgggtcag atccaggaaa aactggaaaa
     1741 aggaccgaaa ctcaaggcac tgatttcggc gaaaaaaccg ccggaggaaa ttattgagga
     1801 tctctatctg accatcctgt cgcgccgtcc gacgaaagaa gaaaaacaga tcgtctttgc
     1861 gtacggaaac ctcggcactc cggaaaaccc gcccccgccg ggcaagcccg gaaagaccgg
     1921 aaacgagaag cagaagcagc agcgtaaacc cggcgatccc ggacggcaga gcagctggat
     1981 cgacattgcc tgggctctga tcaacagtcc ggagttttta tatcgtcact aggaggctcc
     2041 tatgaatatg acccggcgca acgcattaaa atacgggctc tgcggagcag gcagcgctct
     2101 gctcgccaat aaactgcagg cggcagcccc cttgtccacg ctgccggtca aagccaaatc
     2161 ggtgattcag atctttctgt ggggcgggat gtcgcacaac gacacctggg atcccaagcc
     2221 ggacagcgga cgcgactaca tgggtgattt tatgaaggtt ctccccacca atgtggccgg
     2281 cattcagatc ggacagctct tcccggaact ggcgaaacag gccgataaat tttcgctgat
     2341 ccgcagcatg actcacggga acaacggcca cgaaaccgcc gcctactata tgcaaaccgg
     2401 ccactcgagc aatgagcgga tcacctaccc cagcatcggg gctgtttttt cctacttcaa
     2461 aaaccccggc tacaaaggcc tgattccgcc ttatgtggtg atgacccggc cccagggccg
     2521 ctttgccgaa gagggattcc tggggccgat gtataaaccc ttcgccaccg gcggagaccc
     2581 caatgccgcg cggtttgagg tcgaaggagt catcgccaag ggaatcagcg atgaacgcca
     2641 ggccgcccgg cgcgaactgc tcagaaaaac ggatctgctt ggacaggcca tgaactccgc
     2701 cgtactgaaa gatgcccgca tcgccgaaga ccgggcctat gaactgatcc tcggatccgg
     2761 caaagaagta ttcaacctct ccaatgaaga cgacaaactg cgcgaccgct acggacgcaa
     2821 taccttcggg caggaatgtc ttgccgcacg gcggatggtc gaaaccggcg taccgtacat
     2881 cgtcatcaac tatcccggcg gatgggatac gcacaaaggc cattttgcgc ggatgcgcct
     2941 gcaatgcccg aacctcgacc agggactggc cgccctgctg gaagatctgg caaaccgcgg
     3001 cctgctggac agcacactgg tctggtgctg cggcgaattc ggccgcggtc cgaaagtgga
     3061 ctggcagccg ccgtggaacg gcggacgcaa ccactacgga gcggtctttt cagttctcgt
     3121 ggccggcggc ggattccggg gcggcgcgat cgtcgggtct tcggatgaaa aagccgaagc
     3181 cgtcagcgaa cggccggtct atccggtgga tctgctcggc agtatttacc ggctgggcgg
     3241 catcgatccg gcggccatgc tgccgcatcc gctcgggctc gaagcccgcg ttctcccgga
     3301 aatttccggc accatcaagt cgggcggtct gttgacggaa atcatgtaga aagaacggca
     3361 tgatgaaacc ccggacaaac ggaacagtga aaaaactcct gatcgcggcg atttccggac
     3421 tgcttgcact tcaggcgaac gaagcagcgg cacagacagc gaaaaagcgg atctatatcg
     3481 gctacgtcta tccggccggc ggccggcagg gaaccacctt cccggcgcgg gtcggcgggc
     3541 agcagatcga agacgcctgc ggcgccaccg tctccggcga aggcgttcac gtgaacctgg
     3601 gcaccgtctt ccgcaagttt gatacccagt catcccgtct gatctctgag cagctgaaac
     3661 tcatcaaaaa agaaaagtca gacctggacg aaacgaccct taaaataaaa gaacagatcg
     3721 aactgctgat gtccgaggct gtcaaacggg cggccagcga atcactcgcc acggtcatca
     3781 cgctcgatat cactgtcgat gcggatgccg ctccgggccc gcgcgaaatc cgcatcatga
     3841 ctccgggcgg catctccaac ccgcttccgt tctatgtcgg cgtcctgccg gagaactccc
     3901 tgccgccgat gaaaacgtct tcgttccaga tcctgggcaa agaacagttg gccttgcgca
     3961 aacgtcctga agaagatgct gaaaaatcaa tcaccctgcc ctgcaccgtt aacgggcaaa
     4021 tcgcctccgg agaaatcaac cgctaccgct tctccgccga caaaggcgac cggctggtca
     4081 tctccacgct cgcccgccag ctgattcctt atattgccga cgctgttccc ggctggttcc
     4141 agccggtgct ctctctgcgc aatgccgccg gcaccgagat cgcctataat gacgactacc
     4201 ggttcaaacc cgatcccgtc attctgtgcg aaatcccgga aaaaggagaa tacatcctct
     4261 cgatcaccga cgccatctac cgcggccggg aagattttgt ttaccgcatc accatcggtc
     4321 aaactccgtt catcaccggc atctttcctc ccggcgcccg cgccggagaa acggtctccg
     4381 ccgacatgag cggctggaat ctggacaatg caaaactgat tctccccggg ccggacgccg
     4441 gggaaagcct ccacccggcc ggtgccgcga gacacgacgt gctctccaat cttattccgt
     4501 tcgccatcga caccctgccc gaatgtactg aacgccggtc caacaacgac ctgaatacag
     4561 cccaggatgt cacgctgccc gtcatcatta acgggcgcat tgaaacgccc ggcgaccggg
     4621 atgtcttccg gttcaccgcc aaaaagggcg ataccgttgt cgctgaagtc aacgccagac
     4681 ggctcgattc accgctcgat tcgctgctga ccctgaccgg cagcgacgga aagataatcg
     4741 ccatcaacga cgaccacagc gatccgggct ccggactgaa cacccaccat gccgactcat
     4801 acatcatgac cgaactgccc gccgacggaa cctactatgt tcagctgacc gacactgcac
     4861 atcagggcgg ttccaataac acctaccgtc tgcgcatcag tccgccgaag cccgacttcg
     4921 cccttcgcac cgaaccctca cacttcgaca ttcgcggcaa caccgcttcc gtgacagtct
     4981 atgccatccg caaagacggc tataacggac cgatcaccct gcgcctcgcc gaaccggacg
     5041 ccgggtttga tgccaaaccg gtcacgctcc ccgccggagc tgaaaaaatc aaattcaaca
     5101 tccgctgcac cgcaaaggat ctgaaaggct ccgttccggc tgtaatcacc ggaaccgcag
     5161 aaatcggcgg acggcaaacg atccggcagg ccgtcccggc tgaagactgg atgcaggcct
     5221 ttctatggcg gcacctggtt ccggcacagg aactcgtcat ctaccggtac gatccctcca
     5281 tgtcgccgga cacacgccgc cccctgccgg aggacccgga tgtctcgctc tacctgaaaa
     5341 gcaataacag cttaactccg gaacagaaaa agatagccgg tatgatcaaa cagaccgcca
     5401 gcctctacga aaagtggctt ctcacggaca gtttctatca ggaaaccatc aaaggctatc
     5461 tcgagtacac aaccgggaag aagccggaa
//
LOCUS       DAOPZO010000122         5465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_18759_length_5465_cov_7.236784, whole genome shotgun sequence.
ACCESSION   DAOPZO010000122 DAOPZO010000000
VERSION     DAOPZO010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18759_length_5465_cov_7.236784"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PKI68_07660"
     CDS             complement(<1..511)
                     /locus_tag="PKI68_07660"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNX53605.1"
                     /translation="MDIVFSTFNARYSHTALALRCLRANLGGLHDRSVIVEFDNRLSP
                     QAAAEKLLEHNPKIILFSIYIWNLIVTYETMTLLRALRPGLKIIIGGPEVSYEYHDSL
                     TVQLADHLVCGEGESVIEELCRTLLDEQGRLEAGDTSSVPPASSRPCPDSVPPASSRP
                     RFNSFQEVHQ"
     gene            554..1108
                     /gene="def"
                     /locus_tag="PKI68_07665"
     CDS             554..1108
                     /gene="def"
                     /locus_tag="PKI68_07665"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNX53606.1"
                     /translation="MASPICVYGNPVLRKKSEPVKEVTAEIRTLADRMLGVMYAQDGI
                     GLAAEQIGHTEAIFVLDVPADADKNEQGLPNNPGVSMPQAFINPEIIGESEETAIAEE
                     GCLSFPKLYVPVERPSEVVLRYLDREGHTQVLNAKGLLARAIQHELDHLNGVLLIDHM
                     TPAMKLKHALKLRGIRKWYKSRGY"
     gene            complement(1102..1911)
                     /locus_tag="PKI68_07670"
     CDS             complement(1102..1911)
                     /locus_tag="PKI68_07670"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX53607.1"
                     /translation="MEKRMILRPVLLVIILYLCAVLWMWLRQERYLFLPKHYDPQPEF
                     ERFRWDREISGIRHQGWFLDKGSATTVIYHGGNAEDLAGHCQVMWDGLDANALLVNYR
                     GYGQSEGAPGEKEMVADAIAIFDLFCAEKKIAPSNIFLMGRSLGSGVAIQVAAARPQA
                     AGLILVTPYESIEAMARFRYPWLPAKGIMRHPFHAIKFAPEIKMPALVLLAEFDEVIP
                     VETGRKLGEALGGPKKIITLPMGHMDINEHPGYYGAINRFINPPGLRPELQ"
     gene            complement(1896..2420)
                     /locus_tag="PKI68_07675"
     CDS             complement(1896..2420)
                     /locus_tag="PKI68_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNX53608.1"
                     /translation="MKFIELAEKRCSIRSYTPAPVSDEQLNEVLEAGRLAPTACNLQP
                     FQFVVVRKKENLAALAAGYPAPWFAEAPVVIAICIQPSKAWKRSRHDDRCLIDVDGAI
                     AADHMTLAAADLGLGTCWVGAFDPKIVRKVLGVPRTVEPLVMLTLGHPNEKGRPKIRQ
                     PLEKMIRHETWKNG"
     gene            2591..3055
                     /locus_tag="PKI68_07680"
     CDS             2591..3055
                     /locus_tag="PKI68_07680"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HNX53609.1"
                     /translation="MSKHKRPGWDDYFMDIAHVVSRRGNCSRRQVAAVIVRDRRIVST
                     GYNGTPRGITNCDEGGCARCNSDAPSGHSLGDCICCHAEENAIVQAAYHGIAVKDGIL
                     YCTMSPCLMCTKMIINAGIAEVVYEHEYHFNDQARALFKEAGVICRKLIREG"
     gene            3061..3855
                     /locus_tag="PKI68_07685"
     CDS             3061..3855
                     /locus_tag="PKI68_07685"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNX53610.1"
                     /translation="MKRFGSILTGLFLLCAAAPAKELPFFVGEELIYSITWNAIPVAW
                     TRAVTEMDTYEGRDVLALRLYTKSYPFFDHICKVDDFHESLIDPETYLPIRYERNVRE
                     RNYRCHEVTTFDFNTLKAHYVHLLNGKEKTYDIQPGMRDMFSFMYFMRSVKLEENSET
                     TYQVMADEKVYDLFLQTYGLKPIDLPHYDKDVPGLEVRPTAKFDGLYVRKGKATVWIS
                     RDPRRLLMLARLSTPFGRVSVTLHEVNGPGEDFWIKEKKDGDETDN"
     gene            3839..4864
                     /locus_tag="PKI68_07690"
     CDS             3839..4864
                     /locus_tag="PKI68_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HNX53611.1"
                     /translation="MKLTINRAQEIFEKMAGQRVLVAGDLILDRFIYGTVSRVSPEAP
                     IPVVEVTGEKYMPGGAGNVCANVAALGGRCLAAGILGQDAHGAELKRVFGAMGIDCGA
                     VYELPGSQTTVKTRVIAERQQVVRVDRERYPDWTGADKEKFIAKVCAAVKNSTAVIVE
                     DYGKGTLEQDVVDIIVSTAHEAGIPVGYDPKDTHPLHVGGITFASPNRRETFAVAGMP
                     YVRPAANPLEDKPLIEAGRILQERWEAEVLLVTLSEHGMLLLEQGAAPQHIPTRAREV
                     FDVSGAGDTVIAAYMLALAAGASFLEAAELANFAAGVVVGKIGTACCTQTELLAYMGT
                     FTSGSAA"
     gene            4861..>5465
                     /gene="kdsB"
                     /locus_tag="PKI68_07695"
     CDS             4861..>5465
                     /gene="kdsB"
                     /locus_tag="PKI68_07695"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNX53612.1"
                     /translation="MSRVIGVIPARWASTRLPGKPLAMIAGKPMIQRVAEQVVKAKSL
                     DAVLVATDDQRIVDAVASFKIPGVKAVMTRADHPSGTDRIAEAVAGEECDVLINIQGD
                     EPLMDPALIDRLAAVMTSGDWDMATAAAPIKTEADLKNPAVVKAVFARDGQALYFSRA
                     TIPFIRDAATKVDEASSLIFPNDGMRLEASSTIYWRHIGIYA"
BASE COUNT         1124 a         1514 c         1724 g         1103 t
ORIGIN      
        1 actggtgaac ctcctgaaaa gagttaaagc gaggccggct ggaagccggc ggtacagaat
       61 cggggcaggg ccggctggaa gccggcggta cggaggatgt atcgccggct tccagccggc
      121 cttgttcatc cagaagcgtt cggcacagtt cttcgatgac gctttcgcct tcgccgcaga
      181 ccagatgatc ggccagctgg acggtcagcg agtcgtggta ttcgtaactg acttccggtc
      241 cgccgatgat aattttcagt ccgggccgga gtgcgcgcag gagggtcatt gtttcgtagg
      301 tgacgatgag gttccagatg tagatgctga agaggatgat tttggggttg tgctccagca
      361 gtttctcggc ggcggcctgc ggcgagagcc ggttgtcgaa ctcaacgatc accgagcggt
      421 cgtgcagtcc gccgaggttg gcccgcaggc agcgcagggc gagcgcggtg tgggaatagc
      481 gggcgttaaa cgttgagaaa acaatgtcca tgcgttacaa atacgcgctt tgtaacaggt
      541 tgaggagaga aaaatggcga gtcccatttg tgtttatgga aatccggtgc tgcgcaaaaa
      601 gtcggagccc gtcaaagagg ttacggcgga aatccgcacg ctggccgatc gtatgctcgg
      661 cgtcatgtac gcgcaggacg gcatcgggct ggccgccgaa cagatcgggc ataccgaagc
      721 gatttttgta ctcgatgtgc cggccgatgc tgacaaaaac gagcagggtc tgccgaacaa
      781 tccgggcgtc agcatgccgc aggctttcat caacccggaa atcatcggcg aatcggagga
      841 gaccgcgatt gccgaagagg gctgcctgag ttttccgaag ctctatgttc cggtggagcg
      901 tccctccgaa gtggttttgc gctatctcga ccgcgaaggc catacgcagg tgctcaacgc
      961 caaggggctg ctggcccgcg ccattcagca cgaactcgat catctcaacg gcgttctgct
     1021 gatcgaccac atgacgccgg ccatgaagct gaagcatgcg ctcaagctgc gcggcatccg
     1081 gaagtggtat aaaagccgcg gctactgaag ctccggtctg agccccggcg ggttgatgaa
     1141 gcggttgatg gcgccgtaat aaccggggtg ttcgttgatg tccatgtgac ccatcggcag
     1201 ggtgatgatt tttttcgggc cgcccagcgc ctcgccgagt ttccggccgg tttcgaccgg
     1261 aatcacctcg tcgaactccg ccagcagaac cagcgcgggc atcttgattt ccggcgcgaa
     1321 cttaatggcg tggaacggat ggcgcatgat gcctttggcc gggagccacg gatagcggaa
     1381 gcgcgccatg gcctcgatgc tttcatacgg tgtcaccaga atcaggccgg ccgcctgcgg
     1441 ccgcgcggcg gccacctgga tggcgacgcc gctgccgagg ctgcggccca tcaggaagat
     1501 atttgacgga gcgatctttt tctcggcgca gaacaggtcg aagatggcaa tggcgtcggc
     1561 gaccatttct ttttcgccgg gcgcgccttc gctttggccg tagccgcggt aattgacgag
     1621 cagggcgttg gcgtcgagac cgtcccacat gacctggcag tggcccgcga ggtcttcggc
     1681 gttaccgccg tgataaatca ctgtggtggc ggagcctttg tcgaggaacc agccctgatg
     1741 ccggataccg ctgatttctc tgtcccagcg gaagcgttcg aattccggct ggggatcata
     1801 atgttttggc agaaacagat agcgttcctg cctcagccac atccagagga cagcgcagag
     1861 gtacaaaatg ataaccagaa gaaccgggcg cagaatcatc cgtttttcca tgtttcatgc
     1921 cggatcattt tttcaagcgg ctggcggatt ttcgggcggc ctttttcgtt cggatgcccg
     1981 agtgtcagca taaccagcgg ctcaacggta cgcggtacgc cgagcacttt acggacaatc
     2041 ttcggatcga acgcgccgac ccagcaggta ccgagtccaa gatcggcggc ggccagcgtc
     2101 atgtggtcgg cggcgatcgc gccgtccaca tcaatgaggc agcggtcgtc gtggcgtgac
     2161 cgcttccagg ctttggacgg ctggatgcag atggcgatca caaccggcgc ttcggcgaac
     2221 cacggggccg gatagccggc cgccagcgcg gcgagatttt cctttttgcg tacgaccacg
     2281 aactggaacg gctggagatt acaggcggtc ggagcaagcc gtccggcttc cagcacttcg
     2341 ttgagctgct catcggaaac cggagccggt gtatacgagc ggatgctgca tcgtttctct
     2401 gccaactcga tgaatttcat gaggaatcct ttcgtcagac tgtttttaca ggaggccgtg
     2461 aagaaaacca agcccccaaa agatatggag cgggatggga attctctgcg cgctttgcgg
     2521 cataaaatcc ctttggcgtt tgctgccgaa acggttgaat cttgcggggt tatgaaatag
     2581 gatgcgccgc atgagtaaac acaaacgtcc cggctgggat gactatttta tggatatcgc
     2641 ccatgtggtg tcgcggcggg gcaactgctc gcggcggcag gtggcggcgg tgattgtacg
     2701 cgaccggcgg attgtttcga ccggctataa cggcacgccg cgcggaatta ccaactgcga
     2761 cgaaggcggc tgcgcgcgct gtaattcgga cgcgccgtcc ggtcattcgc tgggcgactg
     2821 catctgctgt catgccgaag agaacgcgat tgttcaggcg gcctatcacg ggattgccgt
     2881 gaaagacggg attctctact gcacgatgag tccgtgtctg atgtgcacga agatgattat
     2941 taacgccggg attgccgaag tggtttatga acacgaatac catttcaatg atcaggcccg
     3001 ggcgctgttc aaagaagccg gcgtcatctg ccgcaagttg attcgggaag gttgaatccg
     3061 gtgaaacgtt ttggatccat cctgaccggt ctgtttctgc tgtgcgccgc tgcgcctgcg
     3121 aaggagctgc cgttttttgt cggcgaggaa ctgatttact ccatcacatg gaacgcgatt
     3181 ccggtggcgt ggacgcgggc cgttaccgaa atggatacct atgaaggccg cgatgtgctg
     3241 gcgctgcggc tgtataccaa gagctatccg ttttttgacc atatctgcaa ggtggatgat
     3301 ttccacgaaa gcctgattga tccggagacc tatctgccga tccgctacga gcggaatgtg
     3361 agggagcgga actaccgctg ccacgaagtg accacctttg atttcaatac gctgaaggcg
     3421 cactacgtcc atctgctgaa cggcaaggaa aagacctacg acatccagcc cggcatgcgc
     3481 gatatgttca gcttcatgta tttcatgcgg tcggtgaagc tggaggaaaa cagcgaaacc
     3541 acctatcagg tcatggcgga tgaaaaagtg tacgatctgt tcctccagac ctacggcctg
     3601 aagccgattg acctgccgca ttacgacaag gacgtccccg gtcttgaagt gcgtccgacg
     3661 gccaagtttg acggcttgta tgtgcgcaag ggaaaagcta cggtctggat ctcccgtgac
     3721 ccgcgccggc tcctgatgct ggcccggctg agtactccgt tcggaagagt cagtgtgacg
     3781 ctgcacgaag tgaacggacc gggagaagat ttctggatta aagagaagaa ggacggcgat
     3841 gaaactgaca attaaccgtg cgcaggagat ttttgagaaa atggccggcc agcgtgtgct
     3901 ggtggccggc gacctgattc tggaccgttt catttacggc accgtctcgc gggtttcgcc
     3961 ggaagccccg attccggtgg tcgaagtgac cggcgaaaaa tatatgcccg gcggcgcggg
     4021 caacgtctgc gccaacgtcg ccgcgctcgg cggacgctgt ctggcggccg ggattctcgg
     4081 ccaggacgcg cacggcgcgg aactgaaacg ggttttcggc gcgatgggca tcgactgcgg
     4141 cgcggtctat gaactgcccg gctcgcagac gacggtgaaa acccgcgtga tcgccgaacg
     4201 ccagcaggtc gtacgcgtgg accgcgagcg ctatccggac tggaccggcg cggataaaga
     4261 aaaattcatc gccaaagtgt gcgccgctgt caaaaattcc accgctgtga tcgttgaaga
     4321 ttacggcaaa ggcacgctcg aacaggacgt cgtcgatatc atcgtcagca ccgcgcacga
     4381 agcgggcatt ccggtcggtt acgatccgaa agatactcat ccgctccatg tcggcggaat
     4441 cacctttgcc agcccgaacc gtcgcgaaac ctttgcggtg gcgggaatgc cgtatgtccg
     4501 cccggcggcc aatccgctgg aagacaaacc gctgatcgaa gcgggccgca ttctgcagga
     4561 acgctgggaa gccgaagtcc tgctggtgac gctgagcgag cacggcatgc ttctgctgga
     4621 gcagggcgcc gcgccgcagc atattccgac ccgggcgcgc gaagtatttg atgtcagcgg
     4681 cgcgggcgac accgtgatcg ccgcctacat gctggcgctg gccgccggag ccagcttcct
     4741 cgaagccgcc gagctggcca atttcgccgc cggcgtcgtg gtcggcaaga tcgggaccgc
     4801 ctgctgtacg cagactgaac tgctggccta tatgggaacg tttacttccg ggagcgcggc
     4861 atgagtcgag tgattggagt gataccggcg cgctgggctt ccacgcgtct gcccggcaaa
     4921 ccgctggcga tgatcgcggg caagccgatg attcagcgcg tagccgaaca ggtggttaaa
     4981 gccaagtctc tggacgccgt tctggtcgcc accgacgacc agcgcattgt cgatgccgtt
     5041 gcctccttca aaattccggg cgtaaaagcc gttatgaccc gcgcggacca tccgtccggc
     5101 actgaccgga ttgccgaagc ggttgccgga gaagagtgcg acgtgctgat taacattcag
     5161 ggcgacgaac cgctgatgga tccggcgctg atcgaccggc tggcggctgt catgacttcc
     5221 ggcgactggg atatggcgac cgctgccgcg ccgattaaaa ccgaagccga cctgaaaaat
     5281 ccggcggtcg tcaaagccgt cttcgcccgc gacggacagg cgctttattt ttcccgcgca
     5341 accattcctt tcattcggga tgcagcaact aaagtagatg aggcttccag cctcattttt
     5401 ccgaacgacg ggatgaggct ggaagcctca tctacgattt actggcggca catcggcatt
     5461 tacgc
//
LOCUS       DAOPZO010000123         5425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_18969_length_5425_cov_10.967225, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000123 DAOPZO010000000
VERSION     DAOPZO010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18969_length_5425_cov_10.967225"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PKI68_07700"
     CDS             complement(<1..323)
                     /locus_tag="PKI68_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53613.1"
                     /translation="MKRRIILWTAGILVLLVITRCHFVAPPLRRADAVITVKTTGYCA
                     CMECSGWKFNWFGFPVSPNGKTKVIGRTSSGSSARPGTVAVDPKVFPPGTKFYIPGYG
                     WGRAE"
     gene            complement(494..1663)
                     /locus_tag="PKI68_07705"
     CDS             complement(494..1663)
                     /locus_tag="PKI68_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNX53614.1"
                     /translation="MGEINYGLTEEQLEIRAMLDEFGKERIVPVRAELDDKGIFPSAL
                     LKELAQMDMMGLYIDEAYGGMGVGHLGFCVAVETLSKYCIGVSVSYAANALGADPIII
                     GGSEEQKKKYLSPLASGEKWAAFGLTEPNAGSDAGGIATTAVKKGDKYILNGTKQWIT
                     NGGEADTYTILAVTNKAKGARGVSCFIVEKGMPGFSFGKKEDKLGIRASATRELIFQD
                     CEVPAENLIGKEGMGFLLAMRTFDISRPGIAAQGVGLAQGALDMAVDYARVRHQFGKP
                     IIANQGLQWMLADMAMKVETARAITYAACNTIDGGEKDVSKISAMCKCYAGDVAMSVT
                     TDALQVLGGYGFMREYPAEKMMRDAKILQIYEGTNQIQRDVIGAALIKEYASKAK"
     gene            complement(1685..2605)
                     /gene="trxB"
                     /locus_tag="PKI68_07710"
     CDS             complement(1685..2605)
                     /gene="trxB"
                     /locus_tag="PKI68_07710"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNX53615.1"
                     /translation="MENIVIIGTGPAGLTAAVYSARANLNPLVIEGFQPGGQLTTTTD
                     VENYPGFPEGISGTELMDLTRKQAERFGARFKTGEVVSACLTCLPHNITLSDGEKIEA
                     KTIIIATGASAQYLGIESEQKLIGRGVSGCATCDGAFYRDVPVAVVGGGDTAMEDALF
                     MTHFASKVTVIHRRDQFRASKIMVDRLLAHPKIEVIWDSVIEEVLDVSKNEVTGLKIR
                     NVKTSAVSELQVSGLFVAIGHKPNTKAFAGQLDMDHAGYLIAKNTRTNVDGVFAAGDV
                     QDAVYRQAITAAGSGCMAALEAERYLGSME"
     gene            complement(2624..3178)
                     /locus_tag="PKI68_07715"
     CDS             complement(2624..3178)
                     /locus_tag="PKI68_07715"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HNX53616.1"
                     /translation="MSKDASIIPIERVEQSIYLIRGHKVMLDRDLAALYGAETRMLNQ
                     AVKRNEARFPSDFMFELTREEVMRISQIVISSESPDIKYSKRVFAFTEQGVAMLSGVL
                     KSSRAVEVNIAIMRTFVKLRRMLESSEELARKLAELESKYDEQFRIVFEAIHELMIPP
                     EPKKNPIGFHVREEPGVYTVRKIS"
     gene            complement(3190..4188)
                     /locus_tag="PKI68_07720"
     CDS             complement(3190..4188)
                     /locus_tag="PKI68_07720"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNX53617.1"
                     /translation="MSTKKEIWVYAEQEHGKLAGVVFELLSEGRKLAAKSGFTLCAVL
                     ASANGKPMHQELFNYGAKKIYNVEDPRLETYQNDYFSKVMADLIKAQNPEIVLYGAST
                     IGRSLAPTVAVMGWAGLTADCTELDFDTERQCLLQTRPAFGGNIMATIICPDHRPQMS
                     TVRSNVFKKERVSENETGEVVDVKIDLSGVRERMKRVESVVEEVNTVDLKAADYIVSG
                     GRGIGGQENFKVIEGLANALGGAVGASRATVDAGWISHHHQVGQTGKTVCPLVYVACG
                     ISGAIQHLAGMQSADLIIAINKDPDAPIFDVADFGLVGDLHQIVPEFTKQINALKS"
     gene            complement(4185..4976)
                     /locus_tag="PKI68_07725"
     CDS             complement(4185..4976)
                     /locus_tag="PKI68_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNX53618.1"
                     /translation="MAMHIVVCIKQVPDSDKVTIDRETNRLNRTGVPSIINPFDENAL
                     EMALQLKDKHGGKITVISMGPPQAEEALRTALSHGADDAILVSDRKFGGADTWATSYT
                     ITLALKKLTEPVDLILFGKQAIDGDTAQVGPGVAHFYGVPMITYAKSCEVTDKGFRVH
                     QVTDHGYNVWDVEKPAALTVVKEANSLRMPSLKKKMAAKKAEIPVWGVEQLQPEESKI
                     GLAGSPTKVNKVFAPPVKLNKEILSGDPADMVKELIFKLREKKVL"
     gene            5124..>5425
                     /locus_tag="PKI68_07730"
     CDS             5124..>5425
                     /locus_tag="PKI68_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53619.1"
                     /translation="MKKSLLYFVLSAAAALLSGCSTSAPVTATPPPPASVPEIMPEPA
                     APPVQAPATQSMLLDEMQKTAAAMGEAGGLAAVGTGESKSLELALNKAKVNGRRELA"
BASE COUNT         1087 a         1549 c         1527 g         1262 t
ORIGIN      
        1 tcttcggcgc gcccccagcc gtaaccggga atataaaatt tggtgccggg cggaaagacc
       61 ttcgggtcca cagcaacggt gccgggccgg gcactgcttc cgctggaagt acggccgatc
      121 acctttgttt ttccgttcgg tgaaacagga aagccgaacc agttgaattt ccagccgctg
      181 cattccatgc aggcacagta gccggtggtt ttaaccgtaa tgactgcatc ggcacggcgc
      241 agcggcggcg caacgaagtg acagcgcgtg ataaccagca gtacaagaat gcccgccgtc
      301 cagagaatga tccgccgttt catgcgggca ctttagaaca ggaaaatttt attgaacaga
      361 aggaaacgaa ggtgacgaag ggtacacaga aaaatcggcg ccccctttgt tttccttcgt
      421 ttccttctgt gaaaaatcca caaaaaagcc gccccgtttc cggagcggct tctgttcccg
      481 atgagtggaa actttacttg gccttcgagg cgtactcttt gatgagtgcc gcgccgatga
      541 cgtcgcgctg aatctggttg gtgccttcgt agatctggag gatcttggca tcgcgcatca
      601 tcttttcagc cggatattcg cgcatgaagc cgtaaccgcc gagaacctgc agggcatcgg
      661 tggtgacgga catggcgaca tcgccggcat agcacttaca catggcggag atttttgaga
      721 catccttttc gccgccgtcg atggtgttgc aggcggcgta cgtgatggcg cgcgcggttt
      781 caaccttcat cgccatgtcg gcgagcatcc actgaaggcc ctggttggcg ataatcggtt
      841 tgccgaactg gtggcggacg cgggcatagt cgacagccat gtcgagtgcg ccctgcgcca
      901 gaccaacgcc ctgcgcggcg atacccggac gggaaatgtc gaaggttctc atcgccagca
      961 ggaagcccat gccttctttc ccgatgaggt tttcggccgg aacttcgcag tcctggaaga
     1021 tgagctcgcg ggtggcggaa gcgcggatac caagcttgtc ttcttttttg ccgaagctga
     1081 aaccgggcat tcctttttca acgataaagc aggagacgcc gcgggcgcct ttggctttat
     1141 tggtgacggc cagaatcgtg taggtgtcgg cttcgccgcc gttggtgatc cactgcttgg
     1201 tgccgttgag gatgtatttg tcgcctttct tgacggcggt ggtggcaata ccgcctgcgt
     1261 cggaaccggc gttgggctcg gtcagaccga aagcggccca tttttcgccg ctggcgagcg
     1321 gggaaagata ttttttcttc tgctcttcag aaccgccgat gatgatcggg tcggcgccga
     1381 gggcgttcgc ggcgtaggaa acggaaacgc cgatacagta tttgctgagc gtttcgacag
     1441 cgacgcagaa accgagatga ccgacgccca ttcctccgta ggcttcgtcg atgtagaggc
     1501 ccatcatatc catctgcgcc agctctttga gcagagcgga gggaaagatt cctttgtcgt
     1561 ccagctcggc acgtaccggc acaatgcgtt ctttaccgaa ttcatcgagc atggcccgga
     1621 tttcaagctg ctcttccgtc aatccataat tgatttcgcc catactgttc tccttggttg
     1681 attcctattc catgcttcct aaataacgtt cggcttccag cgcagccata cagcccgatc
     1741 cggcggccgt gatggcctgg cggtaaaccg catcctgcac atcgcccgcc gcgaacacgc
     1801 cgtcgacatt tgtgcgggtg tttttggcga tcagataccc ggcatgatcc atatcaagct
     1861 gtccggcaaa ggccttcgtg ttcggcttat ggccgatggc gacaaacagt ccgctaactt
     1921 gcagttcaga aaccgcactg gttttgacgt tacggatttt caggccggtt acttcgttct
     1981 tagaaacatc cagcacctct tcgatcacgg agtcccagat cacttcaatc ttcggatgcg
     2041 ccagcaggcg gtccaccatg attttggagg cgcggaactg gtcgcggcgg tgaatgacgg
     2101 taaccttgga agcgaagtgc gtcatgaaaa gagcatcttc catcgcggtg tcgccgccgc
     2161 cgacgacggc gaccggcaca tcgcggtaga acgcgccgtc gcaggtggcg cagccggaaa
     2221 cgccgcggcc gatcagcttc tgttcagact cgatgccgag atactgcgca ctcgcgccgg
     2281 tagcgatgat gatggttttc gcttcgattt tttcaccgtc gctcagcgta atgttgtgcg
     2341 gcaggcaggt cagacaggcg gaaaccactt caccggtttt aaaccgcgcc ccgaaccttt
     2401 cggcctgttt acgggtgaga tccatcagct ccgtgccgct gatgccttcc ggaaagcccg
     2461 gatagttttc gacatcggtg gtggtggtga gctgcccgcc gggctgaaag ccttcgatca
     2521 ccagcggatt gaggttggcc cgcgccgaat aaacagcggc ggttaaaccg gcaggccccg
     2581 ttccgatgat gacgatgttt tccaaggtct gtaatctccg cttctacgaa attttccgaa
     2641 ctgtataaac acccggttct tcgcgcacat ggaatccgat cggatttttt ttcggctccg
     2701 gcggaatcat cagttcgtga attgcctcaa agacgatacg aaactgctca tcgtacttcg
     2761 actccagctc cgccagcttt cgcgccagtt cttcgctgga ctccagcatc cggcgcaact
     2821 tcacaaacgt gcgcataatg gcaatattca cctcgaccgc ccgcgaactt ttcagaacgc
     2881 cggagagcat cgcaacacct tgctccgtaa aggcaaaaac ccgctttgag tacttaatat
     2941 cagggctttc agaggatatc acaatttgtg atatcctcat gacttcctcc cgggtcagct
     3001 cgaacatgaa gtccgaaggg aaccgcgctt cgttccgctt gaccgcttga ttcaacatac
     3061 gggtttccgc gccgtagagt gccgccagat cgcgatccag catcacttta tgcccccgaa
     3121 tcaaatagat cgactgctcg acccgctcaa tcggaattat ggacgcgtct ttgctcatct
     3181 ccgtgaattt cagctcttca gcgcgttaat ttgcttcgta aactccggta cgatctggtg
     3241 caggtcgccg accagaccga agtcggctac gtcgaagatc ggcgcgtccg ggtctttgtt
     3301 gatcgcgatg atgaggtccg cgctctgcat accggccaaa tgctgaatgg ccccggaaat
     3361 accgcaggcg acataaacca gaggacaaac cgttttgccg gtctggccga cctggtggtg
     3421 atgagaaatc cagcccgcat caacggtggc gcgcgatgcg ccgactgcgc cgccgagtgc
     3481 attggccaac ccttcgatca ctttaaagtt ttcctgcccg ccgatgccgc gtccgccgga
     3541 aacgatgtag tcggcggcct tgagatccac ggtgttgact tcttcgacga cgctttcaac
     3601 ccgtttcatg cgttcgcgta cgccggaaag gtcgattttc acgtcaacca cttcgccggt
     3661 ttcgttttcg ctgacgcgtt cttttttgaa cacgttggaa cggacggtcg acatctgcgg
     3721 acggtggtcg gggcagatga tggtggccat gatgtttccg ccgaaggccg gacgtgtctg
     3781 aagcaggcat tggcgttcgg tgtcgaagtc gagctcggtg cagtcggcgg tgaggccggc
     3841 ccagcccatg acggcgacgg tcggcgcgag gctgcggccg atggtgctgg cgccgtaaag
     3901 gacgatttcg ggattctgcg ccttgatcag gtcggccatg actttggaaa agtagtcgtt
     3961 ctggtaggtt tccagccgcg gatcttcgac gttatagatt tttttcgcgc cgtagttgaa
     4021 cagctcctga tgcatcggct tgccgttcgc ggaggccagc accgcgcaaa gcgtgaagcc
     4081 cgatttggca gccagtttgc gcccttcgga gagaagttcg aaaacgacgc cggccagttt
     4141 gccgtgttcc tgttccgcgt aaacccagat ttcctttttg gtgctcatag gactttcttc
     4201 tcccgcagtt tgaaaatgag ttctttgacc atgtccgccg gatcgccgga aaggatttcc
     4261 ttattgagct tcaccggcgg agcaaaaacc ttgttcactt tagtcggcga accggccaga
     4321 ccgattttgc tttcttcagg ctgaagctgt tcgacgcccc agaccggaat ttcggctttc
     4381 ttggcagcca tctttttctt gagcgaaggc atccgcaggc tgttggcttc tttaacgacc
     4441 gtcagcgcgg ccggtttttc aacgtcccag acgttgtagc cgtggtctgt cacctgatga
     4501 acccggaagc ctttatccgt tacttcgcag gatttggcgt aggtgatcat cggtacgccg
     4561 tagaagtgag ccacgcccgg accgacctgc gcagtgtcgc cgtcgatcgc ctgcttgccg
     4621 aagagaatca ggtcgaccgg ctcagtcagt tttttgagcg ccagtgtgat ggtgtaggaa
     4681 gtggcccagg tatcggcgcc gccgaacttg cggtcggaaa ccagaatcgc gtcgtccgcg
     4741 ccgtgcgaaa gcgctgtacg cagcgcttct tcagcctgcg gcgggcccat ggagataacg
     4801 gtgattttcc cgccgtgctt atccttgagc tgcaacgcca tttccagcgc attttcgtca
     4861 aaagggttga tgatgctcgg gactccggtg cggttgagac ggtttgtctc gcggtcgatt
     4921 gtgaccttat cggaatccgg tacctgctta atacagacca ctatgtgcat agccattcct
     4981 ttccgtggga aaataggtct cggttataag gatagagctc cgctatctca agccgaaaca
     5041 cccgatttat cggttgccgc ccctttatga atgctctatt ttctttccgg taaaaatacc
     5101 gctaaacgga aatgagttat ttcatgaaaa aatcactgct ttactttgtt ctctccgccg
     5161 ccgctgccct cctgtccggc tgttcaacca gcgcccccgt aaccgcgaca cccccccctc
     5221 cggcatccgt gccggaaata atgccggagc cggccgcgcc gccggtgcag gcgccggcaa
     5281 ctcaatcgat gctgctcgat gaaatgcaga aaacggccgc cgcgatgggt gaagccggcg
     5341 gactggccgc ggtcggcacc ggcgaatcca aaagcctcga actggcgctt aacaaagcca
     5401 aagtcaacgg acgccgggag ctggc
//
LOCUS       DAOPZO010000124         5312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_19560_length_5312_cov_9.929618, whole genome shotgun sequence.
ACCESSION   DAOPZO010000124 DAOPZO010000000
VERSION     DAOPZO010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19560_length_5312_cov_9.929618"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PKI68_07735"
     CDS             <1..225
                     /locus_tag="PKI68_07735"
                     /inference="COORDINATES: protein motif:HMM:NF015050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53620.1"
                     /translation="CGVNDLPLSMILSWYEQKAVCILLTLLHLGIKNIKVGPSLPAFI
                     TPAVLNVLVEKFNIAPTTTVEADLKQCLAK"
     gene            309..3014
                     /gene="nirB"
                     /locus_tag="PKI68_07740"
     CDS             309..3014
                     /gene="nirB"
                     /locus_tag="PKI68_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HNX53621.1"
                     /translation="MPVKERLIVVGNGMAGGKLIEEILSRGAERYAITVIGDEPCGNY
                     NRIKLVVKLKEPALPDFFLNTPEWYDKNGIEALLGAVVTGIDRTGKTVTLQDGRMLAY
                     DKLVLATGSHPFIPPMTGLDLPGVFSLRKLQDVDHIRDFLKDKSHTTVLGGGLLGLEL
                     ALMLRLMGKQVIVSHLMPSLMELQLPEEAGGYLKRHLEALGIRFVMGTYITELLGSTA
                     GVEEARFKDGSSIKTDAVFFNCGIRPNKELAEKAGLVFNRGIAVNDRLQTSDPFIYAC
                     GECIEFKGETWGLVAPVYEQARTLAAVLCGEETVYTPSAPVPARLKSDIPVISMGRFK
                     PQAGDEVVQYTDPHGAIHKQLIICDDLLKGAVLVGEDLNADVIELHYTARIPVPARRA
                     DLLFPGARAGETMIDGKQIPDGAQVCDCNGVSAGKIRRSIQHGNDTLYKVMLNTKAGT
                     GCGNCKNKIKALLISEVGELRIDPAEKYYAAGVPMNREELTAFILANNLRSVSQVLAS
                     VPNAVDDSKTRMALDYLLNYIWMSDYLVEDDARCANDRYSGNIQNDGRFSVIPSMAGG
                     QTTGRHLRAIADVADKYGALIKVTGADRIGLYSIDKKDLKNVWDELQMGSGHAFTKCF
                     RACKSCMGSTHCRFGLMDSLELGRRMGERYRGIMSPAKVKMGVSGCPRNCSEATIKDF
                     GVVAVEGGWDIYIGGNGGSQVYVAHKITQVKTDDDVVRLADRYYEYYCRHAKYGERTA
                     HFVERLGLKTITDAILHAPDKELLELETRFEQVRANYKDPWETAVAPVSREPVPHNQV
                     TENGFTQVALVEEVPPGSSRQFDVAGHPVAVFHTRSGEWIAADGICPHKQGPIVDSIY
                     GNGRLVCPLHSYSFDIKTGACDNPDIGNLKIFAIEIRDGFIFVKP"
     gene            complement(3427..3645)
                     /locus_tag="PKI68_07745"
     CDS             complement(3427..3645)
                     /locus_tag="PKI68_07745"
                     /inference="COORDINATES: protein motif:HMM:NF014821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HNX53622.1"
                     /translation="MKKLAIIAAVMLSTALVSTAADPAVTYKGNPDSKIFHLSSCHHA
                     TNANCTATFTTHDAAAGAGYTPCKVCKP"
     gene            3825..>5312
                     /locus_tag="PKI68_07750"
     CDS             3825..>5312
                     /locus_tag="PKI68_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNX53623.1"
                     /translation="MNKKLPELLAPAGTAESAWAALAYGADAVYAGLPRFSARAEAGN
                     FTAEALDELIGYAHHLGRRVYITFNTLVQQRELAAALETLALIRDLNADGVIVQDMGV
                     VRLAQKNFPSLRLHASTQLTVHNLEGAQQLASLGFKRVVLARELGLQAIEHISRNCGI
                     ETETFIHGALCYSYSGLCLFSSHLLGRSGNRGRCAYCCRQPFESNGGKTLPFSMKDFC
                     AADHLPELISAGVASLKIEGRMKSPLYVAAVTDFYRKALAGDTADSAQRLSDIQTIFG
                     RPSTALYLEDPESDPVDPVNDGHRGAQIGTVKAVAGPWLLLRSNRALQKHDGLKVAMP
                     GETSAYGFAADEMRLASDPESKRRFELPADAEIALKLPADAPRLEKGAAVYCSYSQAV
                     RQRYEFAAPRPGQFRQRRPVNVQIFQSPEVLRFVASADGAQSELLLSGPFEAAKNPEK
                     AAAALRQCFEKLGDTDWKLGELTLDGAAVFVPVSVLNEARRRLISD"
BASE COUNT         1134 a         1520 c         1581 g         1077 t
ORIGIN      
        1 tgcggcgtca acgacctgcc gctctcgatg atcctgtcgt ggtacgaaca gaaagccgtc
       61 tgcattctgc tcacgctgct gcacctcggc atcaaaaaca tcaaagtggg accgagcctg
      121 ccggccttca tcacgcccgc cgtactgaac gtgctggtgg aaaaattcaa tatcgcgccg
      181 accaccacgg tcgaagcgga cttgaagcag tgtctggcga aataacggcc tgaatcaaaa
      241 tcggcacaaa gaactgaaac cctttgtgcc gaaacctttt aatcggcaga gacgaaagaa
      301 ggtgcgccat gccagtaaaa gagcggctca tcgttgtcgg caatggaatg gccggcggga
      361 agctgattga ggaaattctg agtcgcggcg cggaacgtta cgccataacg gtcatcggcg
      421 acgagccgtg cggaaattac aaccgcatca aactggtggt gaagcttaag gagccggcgc
      481 tgccggactt tttcctgaac acgccggagt ggtatgacaa gaacggtatc gaagcgctgc
      541 tgggcgccgt ggtcaccggt attgaccgca ccggaaaaac cgtaacgctg caagacgggc
      601 ggatgctggc ctacgacaag ctggtgctgg cgaccgggtc gcatccgttt attccgccga
      661 tgaccgggct ggatctgccc ggcgtgttct cgctgcgcaa gctgcaggat gtggaccata
      721 ttcgcgactt ccttaaagac aagagccata cgaccgtgct gggcggcggc ctgctggggc
      781 ttgagctggc gctgatgctg cggctgatgg gcaagcaggt gatcgtttcg cacctgatgc
      841 cgtcgctgat ggagctgcag ctgccggaag aagcgggcgg ctatctcaag cgccatctgg
      901 aagcgctggg catccggttc gttatgggaa cttacatcac cgaactgctg ggttccaccg
      961 ccggcgtgga agaagcccgt ttcaaagacg gctcctcaat caaaaccgat gcggtctttt
     1021 tcaactgcgg catccgcccg aacaaagagc tggccgaaaa agccggattg gttttcaaca
     1081 ggggcatcgc ggtgaacgac cggttgcaga ccagcgatcc gttcatttac gcctgcggcg
     1141 aatgtattga attcaaaggg gaaacgtggg ggcttgttgc tccggtttac gaacaggcca
     1201 gaacgctggc ggccgtgctg tgcggcgaag aaaccgttta cacgccttcg gcaccggtgc
     1261 ccgcccggtt gaagtcggat attccggtca tcagcatggg ccgtttcaag ccgcaggctg
     1321 gcgatgaagt tgtgcagtac accgatccgc acggcgcgat tcataagcaa ctcatcattt
     1381 gcgacgacct gctcaaaggc gcggtcctgg tcggtgaaga ccttaatgcg gatgtcatcg
     1441 agctgcacta caccgccaga attccggttc cggcgcggcg ggccgatctg ctgttccccg
     1501 gtgcacgcgc cggcgaaacg atgatcgatg gaaaacagat tcctgacgga gcgcaggtct
     1561 gcgactgcaa cggtgtatcc gccgggaaaa tccgccgctc cattcagcac ggcaacgaca
     1621 cgctgtataa agtgatgctt aataccaagg ccggcaccgg ctgcggaaac tgtaaaaata
     1681 aaatcaaggc tctgctgatt tccgaggtcg gcgaactgcg gattgatccc gccgaaaaat
     1741 attacgccgc aggcgttccg atgaaccgcg aagagttgac agcctttatc ctcgccaaca
     1801 atctgcgctc tgtttcgcag gtgctggcct cggtgccgaa tgcggtggat gactctaaaa
     1861 cccgcatggc gctcgactac ctgctcaatt acatctggat gtcggattac cttgtcgaag
     1921 atgacgcccg ctgcgcgaac gaccgctatt cgggaaacat ccagaacgac gggcgcttca
     1981 gcgtcattcc cagcatggcg ggcggtcaga ccaccggcag gcacctgcgg gcaatcgcgg
     2041 atgtagctga taaatacggt gcgctgatta aagttaccgg agccgaccgc atcggacttt
     2101 attccatcga taagaaagat ctcaaaaacg tctgggatga actgcagatg ggttccggac
     2161 atgccttcac caaatgtttc cgcgcctgca aatcctgtat gggctcaacg cactgccgct
     2221 tcgggctgat ggactcgctg gaactgggcc gccgtatggg cgagcgctat cgcggaatca
     2281 tgtccccggc gaaagtcaaa atgggcgttt ccggctgtcc gcgcaactgc tcggaagcca
     2341 ccatcaaaga tttcggtgtt gtcgccgtcg aaggcggctg ggacatttat atcggcggca
     2401 acggcggctc gcaggtttac gttgcccata aaatcacgca ggtgaaaacc gatgacgatg
     2461 tcgtgcgtct ggccgaccgc tattatgagt actattgccg ccacgccaaa tacggcgagc
     2521 gcaccgccca tttcgtcgag cgcctcggac tcaaaacgat caccgatgct attctgcacg
     2581 cgccggataa agaactgctc gaactcgaaa cccgctttga acaggtgcgg gcgaactata
     2641 aagacccgtg ggaaaccgcc gtcgctccgg tgagtcgcga accggttccg cacaaccaag
     2701 tcacggaaaa cggctttaca caagtggcgc tggtcgaaga ggttcctccc ggctcatcgc
     2761 ggcagttcga cgtggccggt catccggtcg cagtttttca cacgcgcagc ggcgaatgga
     2821 ttgccgccga cggaatctgc ccgcacaaac aaggcccgat cgtggacagc atctacggca
     2881 acggacggct cgtctgtccg ctgcacagct actccttcga cataaaaacc ggcgcctgtg
     2941 acaaccccga catcggtaat ctcaaaatct tcgccatcga aatccgcgac ggcttcattt
     3001 tcgtgaaacc ctgaacgata gtttgataca cactgcaaca agttgtgaaa aagcgcgcag
     3061 gcctcatgaa ataaagcggt atgaagcact attttcccag aatgaaaacg aagtgaaaat
     3121 gtcctccgcg gaaaccgctt cataaaatat ggtagtcatt ggccggcagt tttctgggca
     3181 ttggaatcat tgcgggctta cgtcatctgc ccgattttgt tgcggttgat aactttattc
     3241 cggtcggttc attcggtgca tcggctgtat tggcgtacag gggttccaat ggctgacaat
     3301 atgtctggag accaaaaaag gcattatcct gtgtattgca tttagatgtt cgagcgaatt
     3361 cagcctgcaa aaagacgccc cggattgctc cggggcgtct ttccggcagg gggtataacc
     3421 gcagtcttac ggtttgcaga ctttacaggg cgtatatccc gcgccggctg cggcatcgtg
     3481 tgtggtgaac gtagcagtgc agtttgcgtt cgtggcgtgg tgacagctcg acaggtggaa
     3541 tatcttgctg tccggattgc ccttgtaagt gacggccggg tcggcggcag tggaaacaag
     3601 agccgtgctt aacattacag cggcgataat cgctaacttt ttcattccgt ctccttgtgt
     3661 tattctgttt tacaccaata cgatcccgta tcgtatttgc cggaaaacgt accagagttt
     3721 taaagaattg aaagaagttc tcttcttaaa attcagacgg cggcagcggc cggcgatttt
     3781 caaaacttga agcggcagcg gcggcaggat agcgttcggg ccgcttgaat aaaaaacttc
     3841 cagaactcct ggctcctgcc ggaaccgccg aatcggcgtg ggccgcgctg gcgtacggcg
     3901 cggatgccgt ctatgccggc ctgccgcgct tttccgcccg cgccgaagcg ggcaacttca
     3961 cggccgaagc gctcgacgaa ctgatcggct acgcccacca cctcggccgc cgcgtctaca
     4021 tcaccttcaa cacgctggtg cagcagcgcg agctggccgc cgcgctggaa acgctggcgc
     4081 tgattcgcga tctgaatgcc gacggagtga tcgtgcagga tatgggcgtc gtccggctgg
     4141 cgcagaaaaa ttttccgtcg ctccgcctgc acgccagcac gcagctgacg gtgcataatc
     4201 tggaaggcgc gcagcagctg gcgtcgctcg gctttaaacg cgtggtgctg gcgcgcgagc
     4261 tgggcctgca ggcgattgag cacatcagcc ggaactgcgg catcgaaact gaaaccttca
     4321 ttcacggcgc gctctgctat tcctacagcg ggctctgcct cttttcctcg cacctgctcg
     4381 gccgcagcgg caaccgcgga cgctgcgcct actgctgccg ccagccgttc gagagtaacg
     4441 gcggaaaaac gctgccgttt tcgatgaaag atttctgcgc ggcggaccat ctgccggaac
     4501 tcatttccgc cggggtcgct tcgctcaaaa tcgaagggcg gatgaagagt ccgctctacg
     4561 tcgccgcggt caccgatttc taccgcaagg cgctggccgg cgacaccgcc gattccgcgc
     4621 agcggctctc cgatattcag accatcttcg gccggccttc taccgcgctg tatcttgaag
     4681 atccggagtc ggatcccgtc gatccggtca atgacggaca tcgcggcgcg cagatcggaa
     4741 cggttaaagc cgttgccggt ccgtggctgc tgctgcgcag caaccgggcg ctgcaaaagc
     4801 acgacggact gaaggtggcg atgccgggcg aaacgtccgc ctacggtttt gccgccgacg
     4861 aaatgcggct ggcgtccgat ccggaaagca agcgccgctt cgagcttccg gcggacgcgg
     4921 agattgcgct gaagcttccg gcggacgcgc cgcggctcga aaagggggcg gcggtttact
     4981 gttcctattc gcaggcggtt cgccagcggt acgaatttgc ggcgccgcgc cccggacagt
     5041 tccgccagcg gcggcccgtc aatgttcaga tttttcaatc gccggaagtt ctgcggttcg
     5101 tggcgtcggc ggacggcgcg cagtcggagc tgcttttgtc cgggccgttt gaggcggcga
     5161 aaaatccgga gaaagccgcc gccgcgctcc ggcagtgttt cgaaaagctg ggcgacaccg
     5221 actggaaact cggcgaactg acgctggacg gcgcggcggt ttttgttccg gtgtcggttt
     5281 tgaatgaggc ccgccgccgg ctgatttcgg at
//
LOCUS       DAOPZO010000125         5249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_19891_length_5249_cov_8.258375, whole genome shotgun sequence.
ACCESSION   DAOPZO010000125 DAOPZO010000000
VERSION     DAOPZO010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19891_length_5249_cov_8.258375"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..810
                     /locus_tag="PKI68_07755"
     CDS             154..810
                     /locus_tag="PKI68_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX53624.1"
                     /translation="MKKVLLVDDFHLCRTFFAEGLRKISGLTVCESASAEDALKQAEQ
                     ILPAVIILNISMKHCSGMALLQFMHRQFPSAGLLAFSYLHHDHLYAERAICAGASGYI
                     SADEPGSNLLAAVRAVAEGGIYLSPLLRKKICISSGERCQKMESPFDRLSHREFEVFC
                     LTGHGHVPKRIADKLKVSVKTIETYRERIRGKLGLTDGGELLYHATSFIREQSLPQPL
                     "
     gene            885..2723
                     /locus_tag="PKI68_07760"
     CDS             885..2723
                     /locus_tag="PKI68_07760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX53625.1"
                     /translation="MSDFQQVALLLMLTVSVILQFFAAFLALRLIRSSRNNFPWILIS
                     SALFLMAVRRVIVIFHLLDGGIAHLPDLYAEVIAFVISLLLVAGLISIGPLIRRIGSA
                     AAEPEDASFGELFEKITSGVAVYEAVDKGRDFIIRDINPAAERIEKIARQHVAGRRLA
                     DVFPRIEEFGLPDVLRRVWKTGQPENFPLHYYQDNRIAGWRDNFVYRRFNGEVVAVYN
                     DVSTQMHQQEELERRERKFRLLFEQSPLACQSLDAEAKIIEVNPAWLRLTGLASGSAA
                     GRPFSELLTRTGRQHFQECLNGLKTDGSAIGVVLELRRNNGTLLNVEMDAVYSPSRTG
                     GAEQIYCLLHEKSGQGAGPDVKTGSGKQSGAPVSEQLAAERRKLQMQRLSMMGELAVG
                     MAHEINTPLTVARNAFSLMREDMSPASSHYEFAEMASRALSRMANMVEQMCRFHEPAA
                     QECERLNINALLDNTLVLVRSSLRDRCIQVQDERAKTVPSVVLPPGAVMLTLINPVKN
                     SIEAMSMNGILTLRTGPADQGGVFVEIEDNGPGIPQEFLPHLFEPFTTFRQGGAESAG
                     IGLGLAIVRRTLDVLGGSVTVRSGAGMGTCVKIVLPASLPVQGDPE"
     gene            2720..4132
                     /locus_tag="PKI68_07765"
     CDS             2720..4132
                     /locus_tag="PKI68_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNX53626.1"
                     /translation="MTSEADSGKVCRILLADDHDTYRKTISSLFERRGYQVTGASCGD
                     EVLEAAVKQPFDVFVLDYKMPGNEELILVEQLHALQPDVPVILLTGFASLPSAMNAVR
                     LQLFDYIQKGENPDHLLSRVDEAVRHTQLEKKLRDSEARYRLLAENIQDVITVFSADG
                     KALYSSPSIKTVLGYAPDEFCGIDMYALVHPEDRSDLENAMVVLMESGLIVHEARFRA
                     RSGRYVWLSTSVKLLRDDFGAPQELVCSSSDITGRRQAEERIRQLAQHLETVREEERK
                     RLSRELHDDIGQILTALKIDISILMDHCTCGHGIKQKMENMQQLLGNGIHRVHSLCRR
                     LRPGALDDLGLEEALAGLVDDWKQRNKTICNLHVDMDEEMLSDEIKTAVFRIVQEALT
                     NVSRHARSSRVEIHLVSGGSILNFSIGDNGCGMDLGAEQKPMSFGLLGMRERIEALGG
                     ELHIESIPGEGTRIEGTIPL"
     gene            4212..>5249
                     /locus_tag="PKI68_07770"
     CDS             4212..>5249
                     /locus_tag="PKI68_07770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX53627.1"
                     /translation="MKVLVADDSQLIRERLVDRLSRLPHVKVSEAVDTLEALQQMKTF
                     EPDVAVLDIRMPGGGGIRALTEIKKKNPDTTVIIMTNYPYAQYRRKCLEAGADFFFDK
                     SSEFEQIAETVHQLMQSGKVSEVAHRTAAAQLVAAKEELEKAAQRQSDMNLLSPLHKP
                     AVKDAADQTYAMWGKTFDAFPDLVSILDVNHSIIRVNKALADRLGVPAIELTGKKCFE
                     YFHGAKCPVSGCPHDAMLKDFKEHSAELYEEHMGGWFDIRVSPVFEGNVLVGAIHIAR
                     DITAKRKAEQALKASESSYRQLFESMQAGFALHEIICDQNGVPCDYRFLQVNAAFEEL
                     TGLKAENIVGRT"
BASE COUNT         1171 a         1247 c         1548 g         1283 t
ORIGIN      
        1 ttgccgggaa gtctgcgccc cctgaaatga gtaacacagg gaattttttg cgtttataaa
       61 ttattaattc ttttttacct tttaatttgt accggctgtt ttattgtccg ttcgagcgaa
      121 gccggataga aggggtttga tcgtatggac ggtatgaaaa aagtactgct ggtggatgat
      181 ttccatctct gcagaacatt ttttgcggag ggtctgcgga aaatctccgg tctgacggtt
      241 tgcgaatctg cgagcgcaga ggacgccctg aaacaggcgg aacagattct tcccgcagta
      301 attatcctga atatttccat gaagcattgc agcggaatgg cgctgctgca gtttatgcac
      361 cggcagttcc cgtccgccgg cctgctggcc ttttcttatc tgcatcacga tcatttatat
      421 gctgaacggg cgatctgtgc aggagcgtcc ggatatattt cagccgatga acccgggagc
      481 aatctgctgg cggccgtgag ggcagttgca gagggcggca tttacctgag tccgctgctt
      541 cgtaaaaaga tttgtatcag ttccggtgag cgatgtcaga aaatggaatc gccgttcgac
      601 cggctttcgc atcgtgaatt cgaggtgttc tgtctgaccg gacatggtca tgtaccgaag
      661 cggattgccg acaagcttaa ggttagtgtg aagacgattg aaacctatcg ggagcgtatt
      721 cgcgggaagc tggggctgac ggacggcgga gaattgctat accacgcgac aagtttcatc
      781 cgcgaacaga gtcttcctca gccgttatag gctccggcac cttcatttta acggcttgcg
      841 acggcccgcc gcctcgggta atcttgccgg cctatggggt gtaaatgagt gattttcaac
      901 aagttgcttt attgctgatg cttacagtgt cggttatctt gcagttcttt gccgcgtttc
      961 ttgccctgcg gctgataagg tcttcacgca ataatttccc atggatcctg atttcttccg
     1021 ccctgttcct gatggcggtg cgcagggtca tcgttatctt tcatctgctg gacgggggga
     1081 ttgcgcattt gccggatctc tatgcggaag ttattgcgtt cgtgatttcc ctgctgcttg
     1141 tggcgggact gatttcaatc gggccgctta tccgccggat cggttctgcg gcagcggagc
     1201 ctgaagacgc ttcgttcggc gaactttttg agaaaataac cagcggtgtt gcggtctatg
     1261 aggccgtaga taaagggcgc gattttatta tccgcgacat caaccctgcg gctgagcgga
     1321 ttgagaaaat tgcgcggcag cacgtggcgg gacggcgttt ggcggatgtt ttcccccgta
     1381 tcgaggaatt cggccttccg gatgtgctgc ggcgggtctg gaaaacagga cagcccgaga
     1441 attttccgct gcactattat caggataacc gtattgcggg ctggcgcgat aattttgttt
     1501 accgccgctt caacggcgaa gtggtggcgg tttacaacga tgtgtcgacc caaatgcatc
     1561 agcaggagga gttggagcgg cgggagcgta aattccgcct gctttttgag cagtcaccgc
     1621 ttgcctgtca gtcattggat gcggaagcaa aaatcattga agtgaatccg gcctggctcc
     1681 gtcttaccgg gcttgcaagc ggttctgcgg cagggcgtcc gttcagcgag ctgctgaccc
     1741 ggacgggacg gcagcatttc caggaatgtc tgaacgggct gaaaacggac ggatcggcga
     1801 ttggtgtggt gctggagttg cgccggaaca acgggactct tctgaatgtt gagatggatg
     1861 cagtgtattc acccagcaga accggtggcg ctgaacagat ctattgccta ttgcacgaaa
     1921 aatcagggca gggagccggg ccggatgtaa aaaccggttc aggaaagcag tccggtgcgc
     1981 cggtttctga acagctggcc gcagaacggc ggaaactgca gatgcagaga ctgtcgatga
     2041 tgggcgaact ggcggtcggg atggcacatg aaatcaacac gccgttaacg gttgcgcgca
     2101 acgccttcag cctgatgcgc gaagatatgt cgccggccag ttctcattat gaatttgccg
     2161 aaatggcatc gcgggctctg tcccgtatgg cgaacatggt tgaacagatg tgccgctttc
     2221 atgagccggc cgcccaggag tgtgaacggc ttaacattaa tgcattgctt gataacacgc
     2281 tcgtactggt tcgttcttcc ctgcgggatc gctgcattca agtgcaggat gagcgcgcca
     2341 agaccgttcc ttcggttgtg cttcctcccg gagccgttat gcttacgctg atcaatccgg
     2401 tcaaaaacag tatcgaagca atgtcgatga acggcatatt gacgttgcgc accggcccgg
     2461 cggatcaggg cggagtcttt gtcgaaatcg aagataacgg tccgggtatt ccgcaggagt
     2521 ttcttccgca cctgtttgaa ccatttacca cgttccggca aggcggtgcg gaaagcgcag
     2581 gtatcggtct gggtttggca atcgttcgcc gaacccttga tgtgcttggc ggttcggtga
     2641 cagtccggtc gggtgcgggt atgggaacct gtgtaaaaat tgttctgccg gcttcgctgc
     2701 cggtccaggg agatccggaa tgacgtcaga agccgattca ggaaaagtgt gccggattct
     2761 gctggctgac gatcacgata cctaccgcaa aacgatcagc agtttgtttg agcgccgcgg
     2821 atatcaggtg accggtgcat cttgcggcga tgaggtgctt gaggcggcgg ttaagcagcc
     2881 ttttgatgtg tttgttctcg attataaaat gcccggcaat gaagaattaa ttcttgtcga
     2941 acagcttcat gcgttgcagc cggatgttcc ggtgattctt ctgacaggat ttgcctcgct
     3001 gccttcggcg atgaacgctg tccggctgca gctcttcgac tatatccaga aaggcgagaa
     3061 ccccgatcat ttgctttccc gtgttgatga agcggtccgg cacacgcagc tcgaaaaaaa
     3121 gctccgcgac agcgaggccc gctaccggct tctggctgaa aatattcagg atgtgatcac
     3181 cgttttttcg gctgacggaa aagccctcta ttcttcgcct tcgatcaaaa ccgttctggg
     3241 gtatgcgccg gatgagtttt gcgggataga tatgtatgcg ctggttcatc cggaagaccg
     3301 ttcagacctt gaaaatgcga tggttgtttt gatggaaagc ggattgatcg tgcatgaggc
     3361 gcggtttcgg gcccggagcg ggcgatatgt ctggctgtcc acatcggtca aactgctgcg
     3421 tgatgatttc ggtgcgccgc aggaacttgt ctgttcgagc agcgatatta ccgggcgcag
     3481 acaggcggaa gaacgcatcc gtcagctggc gcagcatctg gaaacagtcc gtgaagagga
     3541 gcgtaaacgt ctttcgcgcg aactgcacga tgacatcgga cagatcctta ctgcattgaa
     3601 aatagacata tcgatcctga tggatcactg cacctgcggg catggcataa agcagaaaat
     3661 ggagaacatg cagcagttgc tgggtaacgg aattcaccgg gttcattcac tctgccgccg
     3721 tctgcgtccc ggcgcactcg acgatctggg gcttgaagaa gcgcttgccg gcctggtgga
     3781 tgactggaag cagcgcaata agacgatctg caatttgcat gtagatatgg atgaagaaat
     3841 gctttccgat gagatcaaga ctgccgtttt ccgcattgtg caggaagcac tcaccaatgt
     3901 gtcacgacat gcccgttctt ccagagttga aattcatctg gtttccggcg gaagcatact
     3961 caatttttcg atcggcgata acggatgcgg tatggatctg ggtgccgagc agaaaccgat
     4021 gtctttcgga ctgctcggta tgcgcgaacg tattgaggcg ctcggagggg agctgcatat
     4081 tgaaagtatt ccgggcgagg gaacgcggat tgaaggaacc attccgctgt agtgtagggt
     4141 tttttcttac acgaaaaaaa gctcagacct gacgttttca ttctatatat taagagtact
     4201 ctcctgcggc tatgaaggtg cttgttgcag atgattcgca actgatccgc gaacggctgg
     4261 ttgaccggct ttcgcgactt ccgcatgtaa aagtgtccga ggcggtggat accctggaag
     4321 cacttcagca gatgaaaacc ttcgagccgg atgtagccgt gctggatatc cgtatgccgg
     4381 gcggcggcgg gatcagggcg ctgaccgaaa tcaaaaagaa aaatccggat acaacggtca
     4441 ttattatgac caattatccg tacgctcaat accgccggaa atgccttgaa gccggtgccg
     4501 atttcttttt cgataaatcg tctgagtttg agcagattgc cgaaacagtc catcagctga
     4561 tgcagtccgg gaaggttagc gaagtggccc acaggacagc tgctgcgcag ctggtggcgg
     4621 ccaaagagga gcttgagaaa gcggcgcagc ggcagagcga tatgaatctc ctgagtccgc
     4681 tccacaaacc tgccgtgaaa gacgcggccg accagaccta tgcaatgtgg ggaaagacat
     4741 tcgatgcctt tccggatctc gtttcaattc ttgatgtaaa tcacagcatc atccgtgtga
     4801 ataaagccct ggctgaccgg ctgggagttc cggcgattga gctgaccgga aaaaaatgtt
     4861 ttgaatattt tcatggtgcg aagtgtccgg tcagcgggtg tccgcacgac gcgatgctga
     4921 aagatttcaa agagcactcg gcagagctct atgaagagca tatgggcgga tggtttgata
     4981 tcagggtttc gccggttttt gaaggaaatg ttctggtcgg ggcgattcat attgcacgcg
     5041 atatcacggc gaaacgcaaa gccgaacagg cgcttaaagc cagtgaatca agctaccggc
     5101 agttatttga atccatgcag gcgggattcg cactgcacga gattatctgt gatcaaaacg
     5161 gggtgccgtg cgattaccgt ttcctgcaag tcaatgcggc ctttgaggaa ctcacagggt
     5221 tgaaagctga aaatattgtc gggcgcacg
//
LOCUS       DAOPZO010000126         5080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_20815_length_5080_cov_5.784279, whole genome shotgun sequence.
ACCESSION   DAOPZO010000126 DAOPZO010000000
VERSION     DAOPZO010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20815_length_5080_cov_5.784279"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>918
                     /locus_tag="PKI68_07775"
     CDS             <1..>918
                     /locus_tag="PKI68_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNX53628.1"
                     /translation="AFSVAPAHGWMQTVSHRLFAAANLLNVNVVLEQASQMFQEVSTE
                     ASLMEKFSDLVSEAFGTTRVVFLRPEGSGSYVQAHPVQTNEKPILFGSGNAVVSLLMR
                     DHEAFTVDTLHRMRPSSLVVNALNELESTGSALAIGSFMRKEMKAVLLLFPKKSGRIY
                     DLRDQRALQLLCDQLAVALENANLYTTVQNGKIYNEILLDSLTSGIVAVNSDRMVTVF
                     NQRAQKLTGLSESEVVDEALTALPPALIEGLETILNTKAGFRDKDMFIVIGGEEIPIR
                     VSGSMFRSHTGKLLGALLVFNDMTVLKKME"
     assembly_gap    919..928
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <929..1650
                     /locus_tag="PKI68_07780"
     CDS             <929..1650
                     /locus_tag="PKI68_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX53629.1"
                     /translation="RLSSIGTLSAGMAHEIKNPLVTIKTFTQLLPQQHNDPEFRHTFF
                     DLVGQEVKRIDTIVNRLLNFARPARAALKPVSLHEIIENSLRLVEQQLAQHEIVLKRQ
                     LDAKRHIIEADAEQLNQTFINFFLNATHAMDKGGTLTVRTTVIKHSQDVPLVAGEQSG
                     DSIRVDIQDTGCGIAPEDINKIFDPFFTTKEDGVGLGLSVSHGIIQEHNGIIDVESEK
                     GKGTVFHVQFPLLNPQEKKDE"
     gene            1647..3632
                     /locus_tag="PKI68_07785"
     CDS             1647..3632
                     /locus_tag="PKI68_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX53630.1"
                     /translation="MSTPPVSIMYTQDGVLLQRIRGYLHSRSTVRHISDPSELELLLH
                     QHHDTLLFMDLRAADCMIALPELIREFPDVVIIALGAVRSDPGLKAASSGVYAIESGE
                     VDRLRLQTLFDQAQAHLRLQQENRLLKEGLQRVAVPAAPSRENIAPSLYHFSSAFRRF
                     DNIGIMLESIVEGVASCARVSRVAVFSITPSELYRFRAGIKCMEETRTLEFKKTHPFV
                     QWLQIHAHSISRSMLRHIDSMEERLLLEKTLDLTGTEAILPLFGRERLIGWLCVGRSS
                     SGAPFEQRDIEELTLLAEQVSVTIENSLLHESIAIQKALAENLLQAIPVGIIAAGADG
                     MIRWFNSGAETLLNLTAEDVIGRPIERLSSYIASLLNRCMAGEKAVGPVEWTEPLTRR
                     GLSIQIRRLQQNGQCMGAMAVLNDLTEERILREKQNNLERATFWNELASAISHDVRNP
                     LVAISTFSQLLPERYNDEEFRTQFRDLTTQEVGRLNGMIDQLDEYANPPKLEFAPVNA
                     GELFEMALYKARNSDVSSRDVGVEIQAGLPSIEGDIDVLADSIARLLHNAFTAVEGAA
                     QPRVSLKAFAGKIGTGRSAVIIEVRDNGTGIPAEIRGKVFSPFCTTKARGVGLGLPIV
                     RRTMIDHGGLVTIDSGETGTVICLSLPAVDNGEGAAA"
     gene            3629..4228
                     /locus_tag="PKI68_07790"
     CDS             3629..4228
                     /locus_tag="PKI68_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNX53631.1"
                     /translation="MKKLLIVDDDPHLLESLRVVFGGLYEICTALSAEEAAVLLEQQE
                     IDVMLLDVVLPGLNGIDFLRIVREIRPNLPVVMISGASSIRPVMKALELGASDFIRKP
                     FDIDELRLIVARALHLSDLRNQVAGLERELARRPFVAEPDGRPMKKVLEDFERTLIEK
                     ALERAGGIQTRAAEVLGTTRRILRYRIQKLKIPAGNQPV"
     gene            complement(4216..4389)
                     /locus_tag="PKI68_07795"
     CDS             complement(4216..4389)
                     /locus_tag="PKI68_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53632.1"
                     /translation="MLCGTNWGCLSFLILFPAGIFIWAAGEMRRRASMPKEELEKLEA
                     EEKYGHIDDHQTG"
     gene            complement(4469..4759)
                     /locus_tag="PKI68_07800"
     CDS             complement(4469..4759)
                     /locus_tag="PKI68_07800"
                     /inference="COORDINATES: protein motif:HMM:NF024673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNX53633.1"
                     /translation="MAADYLMETYEVGLRRSCGLLELTTASYAYEPKGRDDTLLIEAL
                     KSMAQQYRRWGYRSIYDRLRLEGWADNHKRVYRVYSEQGLQKRYPSPLHRAG"
     gene            complement(4777..>5080)
                     /locus_tag="PKI68_07805"
     CDS             complement(4777..>5080)
                     /locus_tag="PKI68_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNX53634.1"
                     /translation="GSASPEPRSCSRRPSKRDTEEQIIGILKEAESGLPVDQLIRKHG
                     ISGGTFYRWKSKYGGMDVSEAKRLKALEDENRQLKRLVADLSLDNQMLKDIHSKNW"
BASE COUNT         1094 a         1428 c         1343 g         1205 t
ORIGIN      
        1 gctttttctg tagccccggc gcatggctgg atgcagacag tttctcaccg tctttttgct
       61 gcggcgaatc tactcaacgt aaatgtggtt ctggagcagg ccagccagat gttccaggag
      121 gtttccacgg aggccagcct gatggagaag ttttcagacc tggtgtctga agcttttgga
      181 accacaaggg ttgttttttt gcgtccggaa ggatcgggct catatgttca ggcgcacccc
      241 gttcaaacca atgaaaagcc gattctgttc ggctccggta atgcagttgt ctctctgtta
      301 atgcgtgatc atgaagcttt cacggtagat actctccatc gcatgcggcc gagttcgttg
      361 gtggtaaatg ctctgaacga gcttgagtca acaggttccg cgttggcgat cggaagcttc
      421 atgagaaagg agatgaaggc ggttcttctt ctgtttccga agaagtccgg gcgcatctat
      481 gacctgcgtg accagcgagc cttgcagttg ctttgcgacc agcttgctgt ggcgctggaa
      541 aatgcaaatc tctacacgac ggtgcaaaac gggaaaatct ataacgaaat tctcctcgat
      601 tctcttacca gcggcatcgt tgcggttaat tccgaccgta tggtgacggt atttaaccag
      661 cgggcacaaa agttaaccgg actttccgag tccgaagtgg ttgatgaggc cttgacagcg
      721 ctgcctcccg ccttgattga aggcctggaa accattctga ataccaaggc cgggtttcgt
      781 gacaaggata tgtttatcgt aatcgggggt gaagaaatcc cgattcgtgt cagcggatcc
      841 atgttccgca gtcataccgg caagctgctg ggtgcactgc tggtgtttaa cgatatgacg
      901 gtgctcaaga agatggaann nnnnnnnnac cgcctttcaa gcatcggaac actctctgcc
      961 ggaatggcgc atgaaatcaa aaatccgctt gtgacgatta aaacgttcac gcagcttctg
     1021 ccgcagcagc ataacgatcc ggaattccgg catacctttt tcgatctggt cggacaggaa
     1081 gttaaacgca ttgataccat tgttaaccgc ctgctcaact ttgcccgtcc ggccagagcc
     1141 gcgctcaagc cggtttcgct gcatgaaatt atcgagaact ctctgcgact ggttgagcag
     1201 cagcttgcgc agcatgaaat tgttttgaag cggcagctgg atgccaaacg gcatattatc
     1261 gaagccgatg ctgaacagtt gaatcaaacc tttatcaact ttttcctcaa cgccacacat
     1321 gccatggata aaggcgggac gttaacggtg cgcacgacag tcatcaaaca ttcgcaggat
     1381 gtaccgctgg ttgccggcga gcagagcggt gacagtatcc gggtggatat tcaggatacc
     1441 ggctgcggta ttgcaccgga ggacataaat aaaattttcg atccgttttt caccaccaaa
     1501 gaagacggcg tcggtctggg gctttctgtg tcgcacggaa ttattcagga acacaacggc
     1561 ataattgatg tcgaaagcga aaaaggaaag ggcaccgttt tccatgtgca gtttcctctc
     1621 ttaaatccgc aggagaaaaa agacgaatga gcacaccgcc tgtcagtatc atgtacacgc
     1681 aggacggcgt actgcttcag cgcatacgcg gctacctcca ctcgcgttca acggtacgcc
     1741 atatcagcga tccgtcagaa ctggaactgc ttctccatca gcatcacgac acgcttcttt
     1801 ttatggattt gcgtgccgca gactgcatga tagcgcttcc tgagctgatc cgagaatttc
     1861 ccgatgttgt catcattgca ttgggcgctg tccgctccga tcccggcctg aaggccgcgt
     1921 catcaggcgt ctatgccatc gaatccggtg aagttgaccg tttgcgtttg cagacactgt
     1981 ttgaccaggc gcaagcccac ctccggcttc agcaggaaaa ccgcttgctc aaagaaggct
     2041 tgcagcgcgt cgctgtgccg gcggcgcctt cccgcgaaaa tattgcgccc tcgctgtatc
     2101 atttttccag cgccttccgc cggttcgaca acatcggcat catgctcgaa agcattgttg
     2161 aaggggttgc cagctgtgcg cgcgtctcgc gtgttgccgt attttccatt acgccgtccg
     2221 agctttaccg ctttcgcgcc ggtatcaaat gcatggagga aacccgcacg ctcgaattca
     2281 aaaaaacgca cccgtttgtc cagtggcttc agatccatgc gcatagtatt tcgcgttcca
     2341 tgcttcgcca tatcgactcc atggaagaac gtctgctgct tgagaaaacg ctcgatctta
     2401 cagggactga ggccattctg ccgcttttcg gacgtgaacg tcttatcggc tggctctgtg
     2461 tcgggaggtc ttcttccgga gctccgtttg agcagcggga tattgaagag ctgacgctgc
     2521 tggccgagca ggtttcggtc accatcgaaa actctctgct gcacgaaagc atcgccattc
     2581 agaaggcgct ggccgaaaac cttctgcagg ctattcctgt cggcatcatc gctgccggag
     2641 ccgacggaat gatccgctgg ttcaacagcg gagctgaaac cctgcttaat ctgaccgccg
     2701 aagatgtgat cggacgtccg atcgaacgcc tttccagcta tatcgcttcg ctgctcaacc
     2761 gctgcatggc cggagaaaaa gcagtcgggc cggttgaatg gactgaacct ctgacccgcc
     2821 gcggactttc catccagatc cgccgccttc agcagaatgg gcagtgtatg ggagccatgg
     2881 cggtactcaa tgatctgaca gaagagcgga ttctgcgcga gaagcagaac aatcttgagc
     2941 gcgccacctt ctggaatgag ctggcctccg ccatttctca cgacgtgcgc aatcctctcg
     3001 tcgccatcag tacgttttcc cagcttcttc cggagcgcta taacgatgaa gagttccgca
     3061 ctcagttccg cgatctcact acgcaggaag tagggcggct caacggcatg atcgaccagc
     3121 tcgacgaata cgccaacccg ccgaaactgg aatttgcgcc ggtgaatgcc ggcgaactgt
     3181 tcgaaatggc tttgtataaa gcacgtaaca gcgatgtctc ttcaagagat gtcggcgttg
     3241 aaatccaggc cggacttccg tctatcgaag gcgatatcga tgttctcgcc gactccatcg
     3301 cccgcctgct gcataacgct tttacagctg tcgaaggagc cgcgcagccg cgagtgtccc
     3361 tcaaagcctt tgcgggtaaa atcggcaccg gacgttccgc tgtcatcatt gaagttcgtg
     3421 ataatggcac cggcattcct gccgagattc gcggaaaagt tttttcccct ttctgcacaa
     3481 ccaaagcccg cggagtaggc ctgggactcc ccatcgtccg ccgcaccatg attgatcacg
     3541 gcggactcgt aaccatcgac agcggcgaaa ccggtacggt catatgtctt tcactgcctg
     3601 ctgttgataa cggggaagga gccgccgcat gaaaaaactc ctgattgtag acgacgaccc
     3661 ccatctgctt gagtcgctac gcgttgtctt cggcggactc tacgaaattt gcaccgcact
     3721 ttccgccgaa gaggccgccg tcctgctcga acagcaggag atcgacgtca tgctgctgga
     3781 cgtcgttctg cccggcctga acgggatcga cttcttacgt atagtgcggg aaatccgccc
     3841 gaatttgccg gttgtcatga tcagcggagc atcctccatc cggcctgtca tgaaagcgct
     3901 cgaactcggc gcctccgatt ttattcgcaa accgtttgat attgatgagc tgcgcctgat
     3961 tgtcgcgcgc gcgctgcatc tgagcgacct gcggaatcag gtggccgggc tggaacgcga
     4021 actggcccgc cgcccattcg tcgctgaacc ggacggacgg ccgatgaaga aagtccttga
     4081 ggacttcgaa cgcaccctta ttgaaaaagc gcttgagcgc gccggcggca ttcaaacccg
     4141 cgccgctgaa gtgctcggaa ccacccgccg cattttgcgc taccgcatcc agaagctcaa
     4201 aattcctgcc ggcaatcagc cggtctgatg gtcatcgatg tgtccgtatt tctcttcggc
     4261 ttcgagtttc tcaagttcct ctttcggcat ggaggcccgg cgacgcattt ctccggcggc
     4321 ccagatgaaa atcccggcag ggaacagaat cagaaaagat aagcaacccc agttcgtgcc
     4381 gcagagcaca accggatttt tccggatatt ggaaacatat atttggtagg gactgttaaa
     4441 actttcgata tatgcgtttt gagtcggttt acccggctct atgaagtggt gatggatacc
     4501 ttttttgtaa gccttgctcg ctgtagaccc ggtaaacccg cttatggtta tcggcccagc
     4561 cttccagccg gagccggtcg taaatcgacc ggtatcccca ccgccggtac tgttgcgcca
     4621 tagattttag cgcctcaatc agcagtgtat cgtcccggcc tttcggctca taggcgtaac
     4681 tggccgttgt cagttcgagc agcccgcagc ttcgtcgcag gccgacttca taggtttcca
     4741 tcagatagtc ggccgccatg cgcttctgtg cgggctttac cagttttttg agtggatgtc
     4801 cttgagcatc tggttgtcca ggctcagatc ggccaccagc cgcttcaact gacggttctc
     4861 atcctccagc gccttcagcc gtttggcttc actcacgtcc atcccgccat acttgctctt
     4921 ccagcggtaa aacgtgccgc cggatatgcc gtgcttgcgg atcagttgat ccaccggcag
     4981 gccggattcg gcctctttaa gaatcccgat gatctgctcc tcggtgtccc gcttcgaggg
     5041 tctccttgag cagctcctcg gctccggact cgccgatccg
//
LOCUS       DAOPZO010000127         4992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_21337_length_4992_cov_5.226048, whole genome shotgun sequence.
ACCESSION   DAOPZO010000127 DAOPZO010000000
VERSION     DAOPZO010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21337_length_4992_cov_5.226048"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="PKI68_07810"
     CDS             <1..1065
                     /locus_tag="PKI68_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX53635.1"
                     /translation="ACIIFSSGSTGTPKGVMLSHANLLSNITALQQVIPFSSQDSIAG
                     ILPLFHSFGYLGTLWWPLLSGTKTVYHPNPLQPAQVVKLVRDEKLTALLATPTLLQTY
                     LRKAEEDDFRSLRYVITGGEKLPETLADSFEEKFGLRPLQGYGATELSPVISLSLPDR
                     TVEGVRIAGGKPSSAGHPLTNVAARVVDLETGAPRPAGETGLLLIKGPNVMQGYLNAP
                     EKTAEVIRDGWYNTGDIARMDEEGFIYLTDRLARFSKIGGEMVPHGAVEEVLQNACGT
                     DTPCVAVVSVRDNEKGEQLAVCYTPEAGDADILHAILKNSRLPNLWIPRRSHFFAVPE
                     FPMLGTGKLDLCTLRRLVSN"
     gene            1227..1814
                     /locus_tag="PKI68_07815"
     CDS             1227..1814
                     /locus_tag="PKI68_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53636.1"
                     /translation="MERSFRTAGYNVINVNYPSRSATIEELTSQVFQSLEENLPNLGT
                     VHFVTHSMGGILLKNYLQDHKIKNLGRVVMLAPPSRGSEVPDKLSGLKLYRWINGPSG
                     NQMGTGTNSLPLRLKEPEMELGVIAGDRSINPILSLLIPGPDDGKVSIARVRPAGATD
                     FIRLHVTHTCMMWNRQVIEQTKAFLKNGAFQKGRS"
     gene            1814..3796
                     /locus_tag="PKI68_07820"
     CDS             1814..3796
                     /locus_tag="PKI68_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HNX53637.1"
                     /translation="MKTKYILCVAALIAAATASGTGLLKPVGALDSGASIKSHQVEVV
                     INNGFAQTTIDQVFANSEDKDFEAIYSFPLPKEASLSELSLWIDGKEVVGEVLEKEKA
                     RKAYEKQVAQGKETALAEKNDFKTFDISVGRIKAGQDTRIRIVYYQPVRIDLNIGRYV
                     YPLAEGGVDDERIAFWETDNRVKEQFSFKLKLKSAFPLKDVRMPGYDQTAQINKTGTN
                     ENQTVYDILIESPEGGATLSRDIICYYRLDDTVPARVELIPYRASPKNTGTFMAVITP
                     AADLKPITEGTDWIFVLDRSGSMSGEKIQTLVKGVGKVLGKMSPKDRFKIVTFNNNAQ
                     NFSGGYISAAPENVQSWIEKVQRIQAEGSTALYDGLKEAYNGLDADRTTGIILVTDGV
                     CNVGPTGHKTFLNLLHQYDIRLFTFVIGNSANQPLMDRLANDSGGFAMNISDSDDIIG
                     RLLQAKIQVMNECMHDVELKFGGEKVHHLTPVAASTLFMGQQLVVFGRYDGSGPVDLK
                     LTAKISGQPHEWNGSAVLPATDTANPELERLWALSSIDEIMEKVREDGEAKPLRERIV
                     ALGTEYSLVTDYTSMIVLDDEAIENEGISRRNLQRVQTERSAQQQRAAAPVQNYRADN
                     GNTFQNRLAPGIGTGPVGPLFIGLIAWLNRRKKIAA"
     gene            3848..4507
                     /gene="rrtA"
                     /locus_tag="PKI68_07825"
     CDS             3848..4507
                     /gene="rrtA"
                     /locus_tag="PKI68_07825"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HNX53638.1"
                     /translation="MNTSFIQIRPKRFEMCLFIAALTAANFALLTGRVCEPLVFFPAK
                     VLSGEWWRILTFPFVHVSFYHLLLDAGAFLLLYHGLRETSAIQRLGMVAACAAGSLCL
                     ALLTSPLAGGLCGLSGIAHGLLAVSALELMQTADKTLQRTGVICLLIVVLKSISEAIT
                     GHVVFETLHLGMVSSPVAICHGGGVLGGILFKILTDCRPFKMWLSGCCCRCQSNPSRV
                     I"
     gene            complement(4760..>4992)
                     /locus_tag="PKI68_07830"
     CDS             complement(4760..>4992)
                     /locus_tag="PKI68_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53639.1"
                     /translation="HSVDLWTSGSVLYGMDGGRPITGLETVLTNGVLEVKFPVPGGKI
                     PSQVIIEEAGGGMDFGSGTLTELFRFAPLERR"
BASE COUNT         1227 a         1393 c         1309 g         1063 t
ORIGIN      
        1 gcctgcatca tcttttcgtc cggctccacc ggaacgccca aaggagtcat gctcagccac
       61 gccaatctgc tgtcgaacat taccgccctg cagcaggtga tcccgttcag cagtcaggat
      121 tccatcgccg ggatcctgcc gctgttccat tcattcggct atctcggtac actctggtgg
      181 ccattactct ccggaactaa aacggtctac cacccgaacc cgctgcaacc tgcgcaggtc
      241 gtgaaactcg tccgcgatga aaagctgacc gccctgcttg cgacgccgac attattacag
      301 acctatctgc gaaaagcaga agaagacgat ttcagaagcc tgcgctacgt cattaccggc
      361 ggcgaaaaac tgcctgaaac gctggcagac tcgtttgagg aaaagttcgg gcttcgtccg
      421 ttgcaaggct acggtgccac cgaactctcc ccggtcattt cgctcagtct gcccgatcgc
      481 acggtcgaag gtgtccgcat agcaggcggc aaaccgtcca gcgctggaca tccgttgacc
      541 aatgtcgccg cacgcgttgt cgatcttgaa accggcgcgc cgcggccggc cggcgaaacc
      601 ggactgttgc tgatcaaagg ccccaatgtc atgcaaggtt accttaacgc gcccgaaaaa
      661 accgccgaag ttattcgtga cggttggtat aacaccggcg acatcgcccg catggatgaa
      721 gaaggcttca tctatctaac tgaccgcctc gcacgcttca gcaaaatcgg cggcgaaatg
      781 gttccgcacg gtgccgtaga agaagttttg cagaacgcct gcggcacaga tacgccgtgc
      841 gttgcagtcg tcagtgtacg cgacaacgaa aaaggtgaac agctcgcggt ctgttacaca
      901 ccggaagcgg gcgacgcgga cattttacac gccatcctca aaaacagccg cctgccgaac
      961 ctgtggattc ctcgccgcag tcatttcttt gctgttcccg aatttccaat gcttggaacc
     1021 ggcaaactgg atctgtgcac actgcgccgg ctcgtcagta attaaggagt aatgccatga
     1081 gagctgccgt catcatccta atcattttat cactcggcgc attcgctttg ctgcgaagaa
     1141 aaagtactgg accgaaagtc ggagaaaaag ccgaaaccgt tgtgctgttg cacggactct
     1201 gccgcagcag ccgggcgatg aatgaaatgg aaaggtcatt ccgaacggcg ggctacaacg
     1261 tcattaacgt gaactatccc tcccgctcag caaccattga agagctaacc agccaagttt
     1321 tccaatcatt ggaagaaaat cttccaaacc ttggaaccgt gcacttcgtc acccattcaa
     1381 tgggcgggat tctgctgaaa aattatctgc aggatcacaa aataaaaaat cttggaagag
     1441 tcgtgatgct cgcgccgccc agtcgcggca gtgaagtgcc cgacaaactg agcggcctca
     1501 aactttaccg atggattaac ggaccgtccg gaaatcagat gggaacggga accaacagcc
     1561 tgccgcttcg gctgaaggaa ccggaaatgg aactgggtgt gattgcgggt gaccgcagca
     1621 tcaacccgat tctttctcta ctgattcccg gtccggacga tggaaaagtt tccatcgccc
     1681 gggtccggcc cgcaggtgca acggacttca tccgactgca cgtcacccac acctgcatga
     1741 tgtggaatag gcaggtgatt gaacaaacca aagcgttcct gaaaaacggg gcgtttcaga
     1801 aaggaagatc gtaatgaaaa caaaatatat cctgtgtgtc gctgcgctga ttgcagctgc
     1861 gaccgccagc ggaaccggac tgcttaaacc ggtcggggcg ctggacagcg gtgcgtcaat
     1921 taagtcgcac caggtcgaag tcgtcatcaa taacggcttt gcccaaacaa ccatagatca
     1981 agtattcgcc aacagcgagg acaaagactt cgaggctatc tattcgttcc cgctaccgaa
     2041 ggaggccagc ctgtcggaac tcagcctctg gatcgacgga aaggaagtcg ttggtgaggt
     2101 actcgaaaaa gagaaggcgc gcaaggcgta tgaaaaacag gttgcacagg gcaaagaaac
     2161 cgcgctggcc gaaaagaacg atttcaagac gttcgacatc agcgtgggcc ggatcaaagc
     2221 cggtcaggac acgcggataa gaattgtcta ttaccagccg gtgcgcatcg acctgaatat
     2281 cgggcggtat gtttatccac tcgcagaagg cggcgtagat gatgaacgaa tcgccttctg
     2341 ggaaaccgat aaccgggtaa aagaacagtt ctcattcaaa ctgaaattaa aatccgcatt
     2401 cccgctcaaa gatgtgcgta tgccgggtta cgaccagacc gcacagatca ataaaaccgg
     2461 aacgaacgaa aatcagacgg tttatgacat tctgatcgaa tcgccggaag gcggagcaac
     2521 gctctcccgc gacatcattt gctactacag gcttgatgac actgtgcccg cccgcgtgga
     2581 attgattcct taccgggcat caccgaaaaa taccgggaca ttcatggcgg tcattacacc
     2641 ggcggcagat ttaaaaccga ttaccgaagg caccgactgg attttcgtac tggatcgatc
     2701 cggcagcatg tcgggagaaa aaattcagac gctggttaaa ggcgtcggaa aagttctcgg
     2761 caaaatgtcg cccaaggacc ggtttaaaat cgtgacgttc aataacaatg cgcagaactt
     2821 ttcgggcggg tatatttccg cggcaccgga aaacgtccag agctggatcg aaaaagtaca
     2881 gcgcattcag gccgaaggca gcaccgcact gtacgacgga cttaaggaag cctacaacgg
     2941 actcgatgcc gaccgtacca ccggcatcat tctggttacc gacggcgtct gcaatgtcgg
     3001 cccgaccgga cacaaaacgt ttctcaatct gctccaccag tacgacattc gtcttttcac
     3061 ctttgtaata ggcaatagcg ccaatcagcc gctgatggat cggttggcta acgactccgg
     3121 cgggttcgcc atgaatattt ccgattccga tgacattatc gggcggctgc ttcaggcgaa
     3181 gatccaagtg atgaacgaat gcatgcatga tgtcgaattg aagttcggcg gggaaaaggt
     3241 tcaccacctc acgccggtcg ccgccagtac actcttcatg ggacagcagc tcgtggtttt
     3301 cgggcgttac gacggctccg ggccagtaga cttgaaactg acggcgaaaa tatccggaca
     3361 gccgcatgaa tggaacggtt cggctgtact gccggccacc gacaccgcca atccggaact
     3421 tgaacggctt tgggctcttt catcaattga cgaaatcatg gaaaaagttc gggaagacgg
     3481 agaagccaag ccccttcgcg agcggattgt cgcgctcgga acggaatatt cactggtgac
     3541 cgactatact tctatgattg tactcgatga tgaagccatc gaaaacgaag gcatctcacg
     3601 tcgcaacctc cagcgcgtac agaccgaacg ctcggctcaa cagcaacgcg ccgctgcgcc
     3661 ggtacaaaac taccgcgctg ataacggcaa tacattccag aaccgtcttg cgcccggtat
     3721 tggaaccggt cctgtcggcc cgttgttcat tggactcatc gcttggctca accgccgcaa
     3781 aaaaatagct gcctgaataa ccccggcagg tcggaaaatc tccggcctgc cgccttcagg
     3841 gaaaatcatg aatacatctt tcatccaaat ccggccgaag cgcttcgaaa tgtgcctgtt
     3901 catcgccgcg ttgactgccg ccaacttcgc gctgctaact ggtcgtgttt gcgaaccgct
     3961 agtgttcttt ccggcaaagg tactttccgg tgaatggtgg cgcatcctga cattcccgtt
     4021 tgtgcacgta agcttttatc atcttctgct ggatgcaggg gccttcctgc tgctctatca
     4081 cggactgcgt gaaacgtctg cgatccaacg gctcggtatg gtcgctgcct gcgcggcagg
     4141 aagtctgtgc ctcgctctgc tgacgtcacc tttagccggc ggactttgcg gactctccgg
     4201 aatcgcacac ggcctgctgg ccgtttctgc gctggaattg atgcagaccg ctgacaagac
     4261 acttcaacga accggggtta tctgcttatt gattgtcgta ttgaaaagca tttccgaagc
     4321 tatcaccggc cacgtcgtct ttgaaactct acatctcggc atggtctctt cgcccgtcgc
     4381 catctgccat ggcggcggtg tattgggagg tattttattc aagatactga cagattgtcg
     4441 ccccttcaaa atgtggttgt cgggatgttg ctgccggtgc cagtcaaatc cgtcacgggt
     4501 catctgagga cagcgcagcc gctctgctgc tgcgcccggt tctgccggca gtctgcccta
     4561 ccatttatat tcgggaattt gatttcggtt gcggttattt atgtgtgaat ttctggcggc
     4621 tgacgacgat cgcggcagcg cgggacatga aacaatacat ctgaccggtg gaggaggtag
     4681 cagatgaaaa cagctacatg tgtatatatg cgtgcaacct cacaggattt taagcaaaaa
     4741 aaccatttcc atctgacttc tagcggcgtt cgagcggcgc aaacctgaaa agctccgtca
     4801 gcgttccgct gccaaagtcc attccgccgc cggcttcttc gatgattacc tgcgacggaa
     4861 tctttccgcc cggaacgggg aatttgactt ccaatacgcc gttcgtcaga accgtttcaa
     4921 ggccggtaat cggccggccg ccgtccattc catagagaac cgatccgctg gtccacagat
     4981 ccacggaatg gc
//
LOCUS       DAOPZO010000128         4954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_21577_length_4954_cov_9.892631, whole genome shotgun sequence.
ACCESSION   DAOPZO010000128 DAOPZO010000000
VERSION     DAOPZO010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21577_length_4954_cov_9.892631"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..3053
                     /locus_tag="PKI68_07835"
     CDS             264..3053
                     /locus_tag="PKI68_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNX53640.1"
                     /translation="MKIVLALLAVIVCGLGLLYGCLTSEWFITGQVLPRVGKAIGQPV
                     TAGRLKVSLFSGVEIQNLAVGSGSNQLLHANTVRLRYRLLRAFQGELAVDEILLDGVK
                     VQVLPDAAGGAKPPQPAKDPSARVKQGEKSKSGGLPLRPVIHNVRIVDASVYVKQAGE
                     NGQPLEFALEKFNLELPEFQLDKPTRIELRGDIRFLQGPEIQLTNAKLKGTLSAEIDS
                     ALQPRSVQLSVRLEEWAGHFRQIALDGRVVAAELALTGDGSRFELQRLRLAESLRGKE
                     EAAIEVNGSGTLNPLTADLNVHVGPIQSSALNLAGALAGGLDFGQTLIDYQGTVRFQA
                     GDRLGSAGKLSIRDAVVTAPSFKRALPPLTVNLDYALAVDLKTRAMTIDRLQSGVRES
                     GREIVSLALSSPLTVSPAASGSGVQAAPAKLQLSIQRFNLLTANAFLPADGGTKLTGG
                     ELSADIRLEIAGQGQQIRAEGSVQIPGLGLDTASRQIRNLDIRDDFSFGLDGLSRLSI
                     RRMELAADSNGRRIAHVNVSGALTLPPASNEISLTIQSGILDIEPLLALAAVPTGGKS
                     PIKPAAPAEPAAKTPKPVFPVTEPPAVDLKGLNLKTDFRFEKIVYRQLLIAPLSGSLR
                     VRDNKVVLDPLELKLNQSPIRMKMSADLGRPGYLYEAHAESGALDLQPVIQSFVPAAF
                     RDTVSASIKSLKLDVKGAGITAANLSRNSDIHFNLETGAIALDRLPYQDVAATALSLP
                     DLRQIRLDQGVLSVNSAANGVSLDGTRLSGPDYLLLAKGKIGFDERLDLQFTPGVGGQ
                     LESKLQSLPVGLLFTERSGKHLIAPPLTYRVAGTITQPSVPDFQKFIKEALLSAAKSQ
                     GQNLLKDAAGQFLKDGKINPQELLKGRTQPQPPTSATNAPSSTTAQPAVQPKTQEQQL
                     IEAGGALLGGFLKKK"
     gene            complement(3398..4123)
                     /locus_tag="PKI68_07840"
     CDS             complement(3398..4123)
                     /locus_tag="PKI68_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53641.1"
                     /translation="MKKLIELTLAGLLSFSIFGCGTTAKFIYPADGKNLTQFNDGPAY
                     QKKVAVVPFEDMRTDKNQSGTMFFYLVPLMPFGWVDYDRPDAARMFMTIAEFEFNSSE
                     DLAKAAALSLRKSGLFQDAFFTFGGDKEKSDYVLEAELLSSHYKGTLYSYGVSFLCPY
                     LWFFGLPAGSSYNELIVSMKMTDLSTGRPVWEKKYDVNRKTIQGYYYNWGDDVRAYSG
                     LMQDIMNDAVKDMNHEFQRKQIK"
     gene            4340..4804
                     /locus_tag="PKI68_07845"
     CDS             4340..4804
                     /locus_tag="PKI68_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNX53642.1"
                     /translation="MENKYPSRLYHKTPAWVEDGSVFHIRIRCSEDNAVSLVQPDVAN
                     EVLESANFYSNAGRWFAHLFLLMPDHLHALISFPKDEAMSRIISEWKRYQTRQHGIQW
                     QENFFDHRIRSTKEYLEKAAYIRRNPVVKGFCETPDAWRWVIDCGTRRPGAF"
BASE COUNT         1073 a         1346 c         1463 g         1072 t
ORIGIN      
        1 tcgtcgaagt gcagggcacc gccgaagaag aacccttcac caaagagcag atggctcaaa
       61 tgatggatct cgccgagaag gggatcaaag aactgctcga ggcgcaaaaa gcctgagtta
      121 tcagttgtcc caaaaagccg gcgacgggaa actgctccgg ttttttgatg tcgggcttca
      181 ggcagtattt tatcgcgcgt ccttgggatg gagcgcgttt tgaggcatga atacctgggg
      241 gatagttcaa gatgaaaacc tggatgaaaa tcgttttagc gctattggcg gtcatcgtgt
      301 gcggactcgg cttgctgtac ggctgtctga ccagcgagtg gtttattacc gggcaggtgc
      361 tgccgcgcgt cggcaaggcg atcggccagc ccgtgacggc gggccggttg aaggtttcgc
      421 tgttttccgg cgtggagatt cagaatctgg cggtcggatc cggctccaat caactcctac
      481 atgcgaatac ggtgcgtttg cgctaccgac tcttgcgggc gtttcagggt gaactggccg
      541 tcgatgaaat cctgctggac ggagtaaagg ttcaggttct gccggatgcc gcgggcggcg
      601 ctaaacctcc gcagccggcg aaagatccgt ccgcccgtgt gaaacagggt gagaaaagca
      661 agagcggcgg gctgccgttg cgtccggtga tccataacgt gcgcattgtg gacgccagcg
      721 tatacgtcaa acaggccggc gaaaacggtc agccgctgga atttgccctc gagaaattta
      781 atctggagct gccggagttt cagctggata agccgacccg tatcgagctg cggggcgaca
      841 tccgcttttt gcagggcccg gaaattcagc tgacgaatgc gaagctcaaa gggaccctca
      901 gcgcggagat cgactccgcc ttgcagccgc gcagcgttca gctgtccgtg cggctggagg
      961 agtgggccgg gcatttccgg cagatcgcgc tggacggacg tgttgtagcg gcggaactgg
     1021 cgctgaccgg tgatggctcc cgctttgaat tgcagcggct gcggctggcc gaaagtctcc
     1081 gtggcaaaga agaggctgcg attgaagtca acggcagcgg aacgttgaat ccgctgacgg
     1141 ccgatttgaa tgtgcatgtc ggccccatcc aatcctccgc cttgaatctg gccggagcgc
     1201 tcgccggcgg actcgatttc ggccaaacgc tgatcgacta tcagggaacg gttcggtttc
     1261 aggccggcga ccggttgggc tcggcaggaa agctgagcat tcgcgatgcg gttgtcacgg
     1321 cgccgtcgtt caagcgcgcg ttgccgccgc tcacggtcaa tctggactat gccttggcgg
     1381 tggatttgaa aacccgggcg atgaccatcg accggttgca gagcggcgtg cgcgaaagcg
     1441 gccgcgaaat cgtcagtctc gctctttcca gcccgttaac agtcagtccg gctgcgtccg
     1501 gaagcggcgt acaggcggct ccggccaagc ttcagctctc cattcagcgg tttaatctgc
     1561 tgacggcgaa cgcctttctg ccggccgacg gcggaaccaa gctgacgggc ggtgaactga
     1621 gcgcggatat tcgtttggaa attgccggcc aagggcagca gatccgtgcg gagggcagtg
     1681 tgcagattcc cggcctcggc ttggataccg cctcccggca aatccgcaac ctcgatatcc
     1741 gcgatgattt ttcgttcgga ctggacggtt tgtcccgtct gagcatccgg cggatggagc
     1801 tggcggcgga cagtaatgga cggcgcatcg cgcacgtgaa cgtcagcggg gctttaacgc
     1861 tgccgcccgc cagcaacgaa atttccctga cgatacagtc ggggattctg gatatcgaac
     1921 cgctgctggc gctggctgcc gtgccgaccg gcggcaaatc gccgataaag cccgcggctc
     1981 cggccgagcc cgcggccaag accccgaagc cggtcttccc tgtaaccgag ccgccggcgg
     2041 tagaccttaa ggggttgaac ctgaaaaccg atttccgctt cgaaaaaatt gtttaccggc
     2101 agctgctgat tgccccgctg tccggctcgc tccgcgtccg cgacaacaag gtggtgctcg
     2161 acccgctcga actgaagctc aaccagtcgc cgatccgtat gaagatgagt gcggacctcg
     2221 gacggcccgg ctatctgtat gaagcgcatg ccgagtccgg cgccctggat ctgcagccgg
     2281 ttatccagag ctttgttccg gcggccttcc gcgacaccgt ttcggcctcc atcaagtcgt
     2341 tgaagctcga tgtcaaaggc gccggcataa cggcggcgaa tctcagccgg aactcggaca
     2401 tccatttcaa tctcgaaacc ggcgcgatcg cgttggaccg cctgccttat caggacgtcg
     2461 cggccacagc cctcagcctg ccggatctcc gccagatccg gctggaccag ggcgtcctca
     2521 gcgtcaactc cgccgcgaac ggcgtgagcc tggacggcac ccgcctgagc ggaccggatt
     2581 acctgctgct ggccaaaggg aaaatcggct ttgatgaacg tctggatctt cagttcacgc
     2641 caggcgtcgg cggacagctg gagtccaagc tgcaaagcct gccggtcggc ctgctcttta
     2701 cggaacgcag cggcaagcac ctgatcgcgc cgccgctgac ctaccgggtc gcgggaacca
     2761 tcactcagcc ctcggtgccg gacttccaga aattcatcaa agaggcgctg ctttccgccg
     2821 ccaaatccca gggacagaac ttattgaagg atgccgccgg gcagtttctg aaagacggca
     2881 aaatcaatcc gcaggagctg ctgaaaggcc gaacgcagcc gcagccgccg acctcggcaa
     2941 ccaacgctcc atcctccaca acggcgcagc ctgctgtgca gccgaaaacg caggaacagc
     3001 agttgatcga agcgggcggg gccctgctgg gcggatttct caaaaagaaa taggctgtcg
     3061 aacggcggct tttgccgccg ttaactcttc aggggtgtga aacaatcaaa cggcagctag
     3121 tgttgcgtct gccttcggaa aagctcgtcg aagtgcaggg cgctgccgaa gccgagcctt
     3181 tcaccaaaga acagagggct cagatggatc tcgccgagaa gggggtttct gagttgttgg
     3241 agtctcagaa agtctaaagt caaaggtctg aacgtcgaag gtcgggatca ggaaaaccgc
     3301 tctggttttt ttgtatcaat agaccgtaga tttcttcaaa acttcgggag ccagattatc
     3361 aaattttcag accgaccctg attacggatg ccagctccta tttgatctgc tttcgctgaa
     3421 actcgtgatt catgtctttg acggcatcat tcatgatgtc ctgcatcaaa ccggaataag
     3481 cgcgaacatc atctccccag ttatagtaat agccctgtat ggttttcctg ttcacgtcat
     3541 acttcttttc ccaaactggg cgacctgtgg agagatcagt catcttcatg gaaacgatca
     3601 gttcattgta ggagcttcct gccggtagtc caaaaaacca aaggtacggg cagaggaaag
     3661 aaaccccata agaataaagg gtgcctttgt aatgcgacga aagaagctct gcttcgagca
     3721 catagtcaga cttttccttg tctccgccga aggtgaagaa ggcatcttgg aataacccgg
     3781 atttccgcag actgagtgcg gcggctttgg caaggtcttc gctagaatta aactcaaact
     3841 cagcaattgt cataaacatt ctggccgcat ctggtcggtc ataatcaacc cagccgaagg
     3901 gcataagtgg gactaggtag aagaacatgg ttccggattg gttcttgtcg gttcgcatat
     3961 cttcaaacgg aacaacggcg acttttttct ggtatgccgg accgtcatta aattgcgtca
     4021 gatttttccc atccgccgga taaatgaatt ttgcagtggt tccgcatccg aaaattgaga
     4081 aggagagaag tccagcaaga gtgagctcga taagtttttt catttatttc cttttgttgg
     4141 cggaaatttt taaacgatat cacggacaag gtaaagggct ttacagccga taggctttta
     4201 gcaggttgat gttcgggcgg caagtgcggc ggacggtaaa gattttgtat aacgtgccgg
     4261 gacggcacgt tccacctggc tgtcgccttt gcgcagaatt taggtggaac gcgccgtccc
     4321 ggcgcgttaa attctttgca tggaaaacaa gtatccttcc cgtctttatc acaagacacc
     4381 ggcctgggtt gaggacggtt cagtattcca cattcgaatc cgttgttctg aagacaacgc
     4441 ggtttctctc gtccagcctg acgttgcaaa cgaagtgttg gaatcggcga acttttactc
     4501 aaatgcgggc cgatggttcg ctcatttgtt tcttttgatg ccggatcatc ttcatgcact
     4561 gatttctttt ccgaaggacg aggccatgtc ccgtatcatc agcgaatgga aacgctatca
     4621 gaccagacag cacgggattc agtggcagga gaacttcttt gatcatcgaa tccggagcac
     4681 gaaggaatat ctggagaaag ccgcctatat ccgccgcaat ccggttgtga aagggttttg
     4741 tgaaactccg gatgcgtggc gctgggtaat tgattgcgga acgcgccgtc ccggcgcgtt
     4801 ttaagtaaga agcgtgcacg gagtgcccgc tccacctggc gcgttccacc ttttacaaag
     4861 aggctaatgc cgcattgaat gtttcactcg ggcgcatgac ggcggcggcg agtttttcgt
     4921 cggggcggta gtagccgccg atatctgccg gttt
//
LOCUS       DAOPZO010000129         4938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_21696_length_4938_cov_8.902519, whole genome shotgun sequence.
ACCESSION   DAOPZO010000129 DAOPZO010000000
VERSION     DAOPZO010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21696_length_4938_cov_8.902519"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1092)
                     /locus_tag="PKI68_07850"
     CDS             complement(22..1092)
                     /locus_tag="PKI68_07850"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNX53643.1"
                     /translation="MNKTSKKFLADMINAISPSGYEHCAAAVWKDYAKPFADTVETDV
                     HGNSHAVINKGGKVKVMLAGHYDEIGFLISHIDDKGFLWIAPIGGWDPQIAQGQRVDI
                     IGNGGKVIRGAIGKIAIHLQEAEQRKKVSEIKDLWVDIGAKNKKEAEKLVSIGDPLVV
                     QYGFEELENGRAVGRAFDDRAGAFAILEAGRLLAKMNTQCEVHIVATVQEEIGLRGAR
                     TAAFGIEPHVGIAVDVTFATDHPNLGSVVNQEGKVELDGGPVITRGPNINHKLFDLII
                     KTAKEEEIPVQIVAEARGTGTDANAIQLNRAGVATALISIPLRYMHSPCELLSLNDLE
                     AVSKLLAAVVKQITPRTDFIPF"
     gene            1229..1531
                     /gene="gatC"
                     /locus_tag="PKI68_07855"
     CDS             1229..1531
                     /gene="gatC"
                     /locus_tag="PKI68_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNX53644.1"
                     /translation="MELLMAHSTHIDVSYVAHLARIHLADGEAPLFQGQLDQVLTYVE
                     QLGELDVSAVEPTAHAMPLVNVLRDDTPRASLDPKTVIANAPAARDGQILVPKIME"
     gene            1566..1946
                     /locus_tag="PKI68_07860"
     CDS             1566..1946
                     /locus_tag="PKI68_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNX53645.1"
                     /translation="MRGLRMLDQEGYDLMAAAFEVYNQMGSGFLEDIYQECFEIELKE
                     RGIPFVPQEQLEVFYKQKILQKKYKPDLLVFGDIIVELKAACNLCGDHEAQLLNYLKA
                     TGKHVGYLINFGNTKKLEWKRMIL"
     gene            1983..3440
                     /gene="gatA"
                     /locus_tag="PKI68_07865"
     CDS             1983..3440
                     /gene="gatA"
                     /locus_tag="PKI68_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNX53646.1"
                     /translation="MLNTKTITELSALLEQGSCTSVQIVNDVLAAIDAQDAKIGAYLT
                     LDRAAALAQAEAADAARAAGKTGPLLGVPLAIKDLLNVKGQPCTCSSKMLEGYVSPYD
                     ATAIAKLRAAGAVFLGRVNMDEFAMGSSTESSAFKKTRNPWNADCVPGGSSGGSAASV
                     AADEAIASLGSDTGGSIRQPAAFCGCVGLKPTYGMISRYGLTAFASSLDQIGPITKTV
                     KDSAILLEAMAGKDDMDSTSVDMEFPKFADLLTDDHSLKGMKLGLPKEYFVEGMDPEV
                     EKSVRDAVEHCKQLGAEIVEVSLPHSKYAIAVYYIIATAEASANLARFDGIRYGLRKD
                     GADPIELYGKTRAAGFGPEVKRRIILGTYVLSSGYYDAYYLRAQKVRTLIRNDFTAAF
                     KQCDAILAPVTPTPAYRIGEKTSDPLKMYLDDILTTPVNLAGICGMSVPCGFSKAGLP
                     IGLQILGDSFKEANILKVGHAYEQTTPWHRQKPKI"
     gene            3536..>4938
                     /gene="gatB"
                     /locus_tag="PKI68_07870"
     CDS             3536..>4938
                     /gene="gatB"
                     /locus_tag="PKI68_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNX53647.1"
                     /translation="MKYEACIGLETHVMQKTQTKMFCGCKHEYGAEPNTNVCPVCLGY
                     PGALPVLNAKAIELCCKAGLLLGCTINPHSKWDRKNYFYPDMSKNYQITQADQPLCLG
                     GQIVTEVNGELKTFTLERIHQEENAAKNTHAASGSLIDYNRAGTALMEIVSTPCMHSA
                     DDAFAYIQALKLIMEYGGISDCDLEKGHMRSDVNVSVRPVGSDKLGAKVEIKNMNSPS
                     FIVEAINYEIERQIEVLENGGKVVQETRGYDSDRGETFSQRTKEDAHDYRYFPEPDLL
                     PVEIPAEQIAKWKAELPELPVQRRERYVKELGLPDYDAKVLTAEKHISDFFDAVCHKT
                     RQYKLASNYMMGKVASLVTEKGVSVVESRLTPETLAQVADLAGGGTISSNAANELIGV
                     LFAEGGDPKAIVEAKGMAQVNDDSALEKWAEEAIAGNAKAVADYKAGNPASINSLMGY
                     VMKQSKGKANPPAVIAML"
BASE COUNT         1074 a         1444 c         1420 g         1000 t
ORIGIN      
        1 acttcatctc tcaaatttcg cttagaacgg gatgaagtcc gtgcgcgggg tgatctgttt
       61 gacgaccgct gccagcagtt tggagaccgc ttcgaggtcg ttgaggctca gcagctcgca
      121 cgggctgtgc atgtagcgca gcgggatgct gatcagcgcg gtggcgacac cggcgcggtt
      181 gagctgaatg gcgttggcat cggtgccggt gccgcgcgcc tcggcgacaa tctgtaccgg
      241 aatttcttct tccttggcgg ttttaatgat caggtcgaag agcttgtggt tgatgttcgg
      301 gccgcgggta atcaccggtc cgccgtcgag ttccactttg ccttcctgat tgaccacgct
      361 gccgagattc gggtggtcgg tggcaaaggt gacgtctacc gcaatgccga cgtgcggttc
      421 gatgccgaac gcggccgtgc gcgcgccgcg cagtccgatt tcctcctgaa ccgtcgcgac
      481 gatatggact tcgcactggg tgttcatttt tgccagcagg cggccggcct ccagaatcgc
      541 gaaggcgccg gcgcggtcgt caaaggcgcg gccgacggcg cggccgtttt ccagctcctc
      601 gaatccgtac tgcaccacca gcggatcgcc gatgctgacc agcttttcgg cctctttttt
      661 gtttttcgcg ccgatgtcga cccacaggtc cttgatttcc gaaaccttct tgcgctgttc
      721 ggcttcctgc agatggatgg cgatttttcc gatggcgccg cggatcactt taccgccgtt
      781 gccgatgatg tcgacgcgct ggccctgtgc aatctgcggg tcccagccgc cgatcggggc
      841 gatccagagg aagcctttgt cgtcgatgtg ggagatcagg aagccgatct cgtcgtagtg
      901 gccggccagc atgactttga ccttgccgcc tttgttgatg acggcgtggg agttgccgtg
      961 gacatcggtt tcaaccgtat cggcgaacgg tttggcatag tccttccaga cggcggccgc
     1021 gcagtgctcg tagccggagg ggctgatggc gttgatcata tcggcgagaa attttttcga
     1081 tgttttattc atggcatcct ctctgaattg agcgcggagt tttccaagga tcgggaaaaa
     1141 cagcaagccg caacgcagag ggctttccaa tgtttaaagc actgtccgct tgccccgttc
     1201 cggcggcctg tctatagtgc cgcctcattt ggagttactc atggcgcact caacccatat
     1261 cgacgtttcc tacgtggcgc atctggcgcg gattcatctt gcggacggcg aggcccccct
     1321 gtttcagggg cagctcgatc aggtgctcac ctatgtcgag cagctgggcg aactcgacgt
     1381 ctcggcggtt gagccgacgg cccacgccat gccgctggtc aacgtgctgc gcgacgacac
     1441 gcctcgcgcc tcgctcgacc caaaaaccgt catcgccaac gcccccgccg cgcgcgacgg
     1501 gcagattctc gttcccaaaa ttatggaata ggagcctgtc cactgatttt cactaatgca
     1561 cactaatgag ggggctgagg atgctggatc aggaagggta tgatctgatg gcggccgcat
     1621 ttgaggttta caatcagatg ggttccggtt ttctggaaga tatctatcag gaatgctttg
     1681 aaatagagtt gaaggagcgg ggcatcccct ttgtgccgca ggagcaatta gaggtatttt
     1741 acaaacagaa gattttgcag aaaaaatata aaccggacct gctggttttt ggcgacatca
     1801 tcgtggagct gaaggcggcg tgtaatcttt gcggtgatca cgaggcccag ttgctgaatt
     1861 atttgaaagc gactgggaag catgttggat atttgatcaa cttcgggaac acgaaaaagc
     1921 tggaatggaa aagaatgatt ctctgaaatc agtgccgatt agcgaaaatt agtggataaa
     1981 aaatgctgaa caccaaaacc attactgaac tttccgcgct gctggaacag gggagctgca
     2041 catctgtcca gatcgtcaac gatgtgctgg ccgccattga tgcgcaggac gcgaagatcg
     2101 gcgcctacct gaccctcgac cgcgccgccg cgctggcgca ggccgaagcc gccgacgcgg
     2161 cccgcgccgc cgggaaaacc ggaccgctgc tcggcgtgcc gctggcgatc aaagatttgc
     2221 tgaacgtcaa agggcagccc tgcacctgtt cgtcgaaaat gctcgaaggc tatgtttcgc
     2281 cgtacgacgc caccgccatc gccaagctgc gcgcggccgg tgcggtattt ctcggccgcg
     2341 tgaacatgga tgagttcgcc atgggttcga gcaccgaaag ttcggctttc aaaaaaacgc
     2401 gcaatccgtg gaatgctgac tgcgtgccgg gcggttcctc cggcggttcc gcggccagcg
     2461 tcgcggccga cgaagcgatt gcgtcgctcg gttccgacac cggcggttcg atccgccagc
     2521 ccgccgcttt ttgcggatgc gtcggcctga agccgaccta cggcatgatt tcgcgctacg
     2581 gtctgaccgc gtttgcgtcg tcgcttgacc agatcggccc gattaccaaa accgtcaaag
     2641 attccgccat tctgctcgaa gccatggccg gcaaggacga catggattcc acttcggtcg
     2701 atatggagtt tccgaagttc gccgacctgc tgacggacga ccacagtctg aagggcatga
     2761 agctggggct gccgaaagaa tactttgtcg aaggcatgga tccggaagtg gagaagtcgg
     2821 tgcgcgacgc ggtggagcat tgcaaacaac tcggcgcgga aatcgttgaa gtcagcctgc
     2881 cgcactcgaa gtatgcgatt gcggtttatt acatcatcgc cacggcggaa gcctctgcca
     2941 acctcgcgcg cttcgacggc atccgctacg gactgcgcaa agacggcgcc gatccgattg
     3001 agctctacgg caaaacccgc gccgccgggt tcgggccgga agtcaaacgc cgcatcatcc
     3061 tcggcaccta tgtgctgtcg agcggctatt acgacgccta ttatctgcgc gcccagaaag
     3121 tccgcacgct catccgcaac gacttcactg ccgcgtttaa acagtgcgac gccattctcg
     3181 cgccggtcac gccgaccccg gcctatcgca tcggcgagaa gaccagcgat ccgctcaaga
     3241 tgtacctcga cgatattctg accacgccgg tcaatcttgc gggcatctgc gggatgagcg
     3301 tgccgtgcgg attttccaag gcgggcctgc cgatcggact gcagattctc ggcgactcct
     3361 tcaaagaagc caacattctc aaagtcggcc acgcctacga acagaccacc ccgtggcacc
     3421 ggcagaaacc aaaaatctag aaatatattc cactgatttt cactgatgag cactaatgga
     3481 aaatgaattc gatcagtgag aatcagtgaa gattagtgga taggaatttt ctgttatgaa
     3541 atacgaagcc tgtatcggac tggaaacgca tgtgatgcag aagacgcaga cgaaaatgtt
     3601 ctgcggctgc aagcacgagt acggcgcgga gccgaacacc aacgtctgcc ccgtctgtct
     3661 cggctatccc ggcgcgctgc cggtcctcaa cgccaaagcc attgagctct gctgcaaagc
     3721 gggcctgctg ctcggctgca ccatcaaccc gcattccaag tgggaccgca aaaactattt
     3781 ctatcccgac atgtccaaaa actaccagat cacgcaggcc gaccagccgc tctgcctcgg
     3841 cggacagatc gtgacggaag tcaacggcga gctgaaaacc tttaccctcg aacgcattca
     3901 tcaggaagaa aatgccgcca aaaacaccca cgccgccagc ggcagtctga ttgactataa
     3961 ccgcgccggc accgcgctga tggagattgt ctccacgccc tgcatgcact cggccgacga
     4021 cgcctttgcg tacattcagg cgctcaagct catcatggaa tacggcggca tctccgactg
     4081 cgacctcgaa aagggccata tgcgctccga cgtcaacgtc agcgtccgtc cggtcggctc
     4141 ggacaaactc ggcgcgaagg ttgaaatcaa gaacatgaac tcgccgagct tcatcgttga
     4201 agccatcaac tatgaaatcg agcgccagat cgaagtgctg gaaaacggcg gcaaggtcgt
     4261 tcaggaaacg cgcggctacg actccgaccg cggcgaaacc ttttcgcagc gcaccaaaga
     4321 ggacgcccac gattaccgct atttccccga acccgatttg cttcccgtcg aaatcccggc
     4381 cgaacagatt gccaagtgga aagccgaatt gccggagctt ccggttcagc gccgcgaacg
     4441 ctacgtcaaa gaactcggac tgccggacta cgacgccaaa gttctcaccg ccgaaaagca
     4501 catctccgat ttcttcgacg ccgtctgtca caaaaccagg cagtacaagc tggcttcaaa
     4561 ctacatgatg ggcaaagtcg ccagtctcgt aacggagaag ggcgtcagcg tcgttgaaag
     4621 ccggttgacg ccggaaactc tggcgcaggt tgccgacctc gcgggcggcg gaaccatcag
     4681 ctccaacgcc gccaatgagt tgatcggagt tctgtttgcc gaaggcggcg acccgaaagc
     4741 gattgttgaa gccaaaggca tggcgcaggt caacgatgac agcgcgcttg aaaaatgggc
     4801 cgaggaagcc atcgccggaa acgccaaagc ggttgccgat tataaagcgg gcaatccggc
     4861 ctccatcaac tcgctcatgg gctacgtcat gaagcagagc aaaggcaaag ccaatccgcc
     4921 cgccgtcatc gccatgct
//
LOCUS       DAOPZO010000130         4929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_21749_length_4929_cov_7.444399, whole genome shotgun sequence.
ACCESSION   DAOPZO010000130 DAOPZO010000000
VERSION     DAOPZO010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21749_length_4929_cov_7.444399"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..332
                     /gene="raiA"
                     /locus_tag="PKI68_07875"
     CDS             27..332
                     /gene="raiA"
                     /locus_tag="PKI68_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNX53648.1"
                     /translation="MQVHITGRHVEISDAIREHIYSKLERTLVDFPRVEDVRVVLDQQ
                     KLQHLAEVLVQGKNVHVEGKSSSENMYTSIDEALEKAEHQLRKLRQKVQDVYHHPGR"
     gene            347..1282
                     /gene="hprK"
                     /locus_tag="PKI68_07880"
     CDS             347..1282
                     /gene="hprK"
                     /locus_tag="PKI68_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNX53649.1"
                     /translation="MAITLKELLDAGSDRLSLELLWGEGRLDREIEEKALNRPGLALT
                     GFFQYFAQRRLQVFGLAEFTYLKSLTSEDREARLRAMFDKNVPGVIISRNRNPLPELR
                     ILAEEYGTPLFRTTMITSHFINECTVILEELTAPRDRIQGTMLEIMGIGVLLQGQAGI
                     GKSETALSLIGRGYSLVADDVTEVVRTSGRVMAFAHELTRYHMEIRGVGIVHVPSLYG
                     VSAIRRDAMLDLVINLHSYKNDADEERTGIQPGEIEVLGIKFPYYALPVSPGRDMANI
                     VEATALNYKLKLLGHDAAKELDEKIIGRFLRKAVK"
     gene            1282..1563
                     /locus_tag="PKI68_07885"
     CDS             1282..1563
                     /locus_tag="PKI68_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNX53650.1"
                     /translation="MAEHKTISREFEVLNKYGIHARPAALLVKAAGKFSCDISIGRKG
                     AEVSAKSIMGLLTIEGHQGSRLIVRAAGHDAEEALLEIAALFENKFFEE"
     gene            1565..3346
                     /gene="ptsP"
                     /locus_tag="PKI68_07890"
     CDS             1565..3346
                     /gene="ptsP"
                     /locus_tag="PKI68_07890"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNX53651.1"
                     /translation="MSEKTTDISGELVLQGIGVSPGVAVGEVVVFAQEGAAVPERTIS
                     ERDIPLEIARFEEALIKTRHQIAEIQRKVSSELGEEHASIFDAHLLVTDDRYFVEEVI
                     RDLRARHCNVDVVLNDVADRYIEMLNRVEDDYLRERAADVRDVTRRIIKNLAGERMDR
                     MDQLERPCIAVAHDFSPSDTAGMNRNIVQALVADVGSSTSHTAIMARALEVPAVVGLH
                     DISAKVSSGETVLVDGSKGQVVLRPSSERLLAYAQKAEDQRLILYELETLKDKPSETK
                     DGYYVPIAANIELPSEIPGITKYGARGVGLFRTEFLFLDKVKLPDEDEQTRVYSEVAD
                     QCAPDNVVIRTLDLGGDKFLSTLKFAPEINPFLGCRAIRFCLANPGFFKTQLRAVLRA
                     AAGRNMSMMYPMVSCLDEVLEANRILEECRQELKKEGLPCAEKLPVGVMIEIPSAALT
                     ADLIAPHVDFFSIGTNDLIQYTLAVDRVNENVAHLYMPTHISILKLIQQTVLAGHACR
                     IPVAVCGQMAATPELVPLLIGLGVDELSISPPSVPMVKEVIRNLHYSECRKLAENAMH
                     TCTAEDILAQCRELLAKTSPEILELIS"
     gene            3417..4601
                     /gene="metK"
                     /locus_tag="PKI68_07895"
     CDS             3417..4601
                     /gene="metK"
                     /locus_tag="PKI68_07895"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNX53652.1"
                     /translation="MSKINVDHVFTSESVSEGHPDKVCDQISDAILDACLAQDSKSRV
                     ACETLVSTNLVVNAGEITCSGWDKINPEAIARKVVREIGYDRAELEFCADTFEYICRL
                     HGQSPDIAQGVDEGRGLFLEQGAGDQGMMFGYASDETAAMMPAPVYYSHLLLDELQKI
                     RKAGTIPYLRPDSKSQVSIKYINGQPHHITSVVISHQTADVPLEKIRKDLVEVIQQVL
                     GPTGLLKDNVEYFINPTGKFVIGGPHGDAGLTGRKIIVDTYGGVGSHGGGAFSGKDPS
                     KVDRSAAYYARYVAKNIVASGLAKKCEVQVAYAIGVAKPLSINVDTYGTGKLEDNRLE
                     AIVSSGEIFDFRPARLIKDLGLLTPKGWNYRDTAAYGHFGRPQFPWEKTDKIAALKAA
                     AK"
     gene            4660..4736
                     /locus_tag="PKI68_07900"
     tRNA            4660..4736
                     /locus_tag="PKI68_07900"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4694..4696,aa:Arg,seq:acg)
BASE COUNT         1176 a         1259 c         1345 g         1149 t
ORIGIN      
        1 gtatgtaaca gagaaaggag aagctcatgc aagtgcatat cacaggacgg catgttgaga
       61 tctcggatgc aatccgggag catatctata gcaaactcga gcggactctg gttgattttc
      121 cgcgcgtcga agacgtgcgt gttgtactgg atcagcagaa acttcagcat ctggcggaag
      181 tgctggtgca gggtaaaaac gttcatgtag aaggcaagtc gtcttccgag aacatgtata
      241 cctcgatcga cgaggcgctt gaaaaggcgg aacatcagct gcgtaaactg cgccagaaag
      301 tgcaggacgt ttaccatcac cccggacgtt aagggaaacc ggtactgtgg cgattacact
      361 gaaagaactg ctggacgccg ggtctgaccg cctttcgctc gaattattat ggggcgaagg
      421 gcggctggat cgtgaaattg aggaaaaagc cctcaaccgg cccggactcg cgctgaccgg
      481 cttctttcag tatttcgccc agcgccgcct gcaggtgttc ggactggccg aatttaccta
      541 tcttaagagt ctgacctccg aagatcgcga agcgcggctg cgcgccatgt ttgataaaaa
      601 tgttcccggt gtgatcattt ccagaaaccg taatccgctt ccggaactcc ggattctggc
      661 tgaagagtac ggcactccgt tgttccgtac gacaatgatt accagtcact ttatcaatga
      721 gtgcaccgtg atactggaag aactgacagc tccgcgcgac cggatccagg gcacaatgct
      781 tgaaattatg ggtatcggtg tgctgcttca gggtcaggct ggaatcggta aaagcgaaac
      841 ggctctttcg ctgatcggcc gcgggtacag tctggtggct gatgatgtga ctgaagttgt
      901 gcggaccagc gggcgtgtga tggcttttgc ccatgaactg acgcggtatc atatggaaat
      961 ccgcggcgtc ggcattgttc atgtgcccag tctttacggt gtatcggcga tccggcgcga
     1021 tgccatgctt gatctggtga tcaatctgca ttcttacaaa aacgacgccg atgaagagcg
     1081 taccggcatt caacccggtg aaattgaggt gctcggaatc aaattcccgt attacgcgct
     1141 gccggtcagt ccgggccgtg acatggcaaa tattgttgaa gcaaccgcat taaactataa
     1201 attaaagctg cttggacatg atgcggctaa agagctggat gaaaaaataa tcggacgttt
     1261 tcttcgcaag gcggtaaaat aatggctgaa cataaaacca tatcacgtga gtttgaggtg
     1321 ctgaacaaat acggtattca cgccaggcct gccgcattgc tggttaaagc cgccggtaaa
     1381 ttttcctgcg atatttcaat cggacggaag ggtgcggagg tttcggccaa gagcatcatg
     1441 ggtctgctta cgattgaggg ccatcagggt tcccgtctga tcgttcgtgc tgccggccat
     1501 gacgccgaag aggctctgct tgaaatcgca gcactctttg agaataaatt cttcgaggag
     1561 taacgtgtca gaaaaaacca cagacatctc cggcgaactt gtcctgcaag gtatcggagt
     1621 gtcgcctggt gttgcggttg gcgaagtcgt ggtgtttgct caggaaggcg cggcggttcc
     1681 cgaacgtacc atcagcgaac gggacatacc tcttgaaatt gcccgttttg aagaagcgtt
     1741 aattaaaacc cgccatcaga ttgccgagat tcagcgcaaa gtaagttcag agctcggcga
     1801 agaacacgcc agtattttcg atgcccacct gctggtcacg gatgaccgct atttcgttga
     1861 agaagtcatc cgcgatttac gtgcaagaca ctgcaatgtc gatgtcgtcc tgaacgatgt
     1921 cgccgaccgc tacatcgaaa tgctgaaccg ggttgaagac gattatctgc gcgaacgggc
     1981 cgccgatgtg cgcgatgtta cccgccgtat tatcaaaaat cttgccggcg aacggatgga
     2041 tcgcatggat cagcttgaac gtccgtgcat tgccgttgcg cacgattttt caccttccga
     2101 taccgcaggt atgaatcgca atatcgtgca ggctcttgtt gctgatgtcg gcagctccac
     2161 ttcgcatacg gcgattatgg cccgtgcact tgaagtgccg gcggtcgtcg ggctgcacga
     2221 tatcagcgca aaggtaagtt ccggagaaac cgtgctggtt gacggcagca aaggacaggt
     2281 tgtactccgg ccctcctctg agcgactcct tgcctatgcg caaaaagcgg aggatcaacg
     2341 gctgattctt tatgagctcg aaactctgaa agacaagccg tccgaaacca aagacggcta
     2401 ttatgtgccg attgcagcca acattgaact gccgtctgaa attcccggca taaccaaata
     2461 cggagccaga ggcgtcggcc ttttccggac agagtttctt tttctggata aggtaaagct
     2521 gccggatgaa gacgagcaga cccgtgtcta tagcgaggta gccgatcaat gcgcccctga
     2581 caacgtcgtg atccgtacgc ttgatctcgg cggggataaa ttcctgtcca cattgaaatt
     2641 tgcacctgaa atcaatccgt ttctaggctg ccgtgccatt cgtttctgtc tggcaaatcc
     2701 cggatttttt aaaacgcagc tgcgggctgt tttgcgtgcc gccgctggac gcaatatgtc
     2761 aatgatgtat ccgatggtca gctgtcttga tgaggtgctt gaggccaacc ggattctgga
     2821 agaatgccgg caggagctca aaaaggaggg gctgccctgc gcggaaaaac tgccggtcgg
     2881 ggttatgatc gaaatacctt ccgcggcgct gacggccgat ctcattgcgc cgcatgtcga
     2941 ttttttcagc atcggcacca atgacctgat tcaatacacg ctggcggttg accgggtgaa
     3001 tgaaaatgtc gcccatcttt atatgccgac ccatatttcc attctgaaac tcattcagca
     3061 gaccgttctg gccggacatg cctgccgcat tccggtcgcg gtctgcgggc agatggctgc
     3121 tacaccggaa ctcgttccgt tgcttatagg gcttggtgtg gatgaactca gtatcagccc
     3181 gccgtctgtg cccatggtta aagaagtaat ccggaatctg cattattccg agtgccgcaa
     3241 gctggctgaa aatgcgatgc atacctgcac ggcagaagat attctggccc aatgccgcga
     3301 actgctggca aaaacgtcgc cggaaattct tgagttgatc agctgaagca ggtacattcc
     3361 actgaaattc atcaatccgg ataaacgaat tgatagagag cagaggagct tgcggtatga
     3421 gcaaaatcaa tgtggatcat gttttcactt ctgaatcggt ctcagaaggc catcctgaca
     3481 aagtgtgcga tcagatttcc gacgccattc ttgatgcctg ccttgcgcag gattccaaaa
     3541 gccgtgtggc ctgcgaaaca ctggtcagca caaatctcgt ggtcaatgcc ggtgaaatca
     3601 cctgctcggg ctgggataaa atcaacccgg aagccattgc gcgtaaagtc gtccgcgaaa
     3661 tcgggtacga ccgcgcagag cttgaatttt gcgccgatac gtttgaatac atctgccgtc
     3721 ttcacggcca gtctcccgac atcgcacagg gtgtggacga aggccgcggc ctttttctgg
     3781 agcagggcgc aggcgaccag ggcatgatgt tcggttacgc ctcggatgaa accgcagcca
     3841 tgatgccggc tccggtttat tacagccatc tgctccttga tgagcttcag aaaatccgca
     3901 aggccggaac cattccttat ctgcggcccg actccaagtc gcaggtttcc attaaataca
     3961 tcaacggtca gccgcatcac atcacgtccg tggttatttc gcatcagacc gccgacgttc
     4021 cgcttgagaa aatccgtaaa gacctggttg aagttataca gcaggttctg ggtccgaccg
     4081 gcctgcttaa agacaatgta gaatacttca tcaatccgac aggtaaattc gttatcggcg
     4141 gccctcacgg cgatgccggg ttgaccggac gcaagatcat tgttgataca tacggcggcg
     4201 tcggcagcca cggcggcggc gcgttttccg gaaaagaccc gtccaaagtc gaccgttcag
     4261 cggcttacta tgcacgctat gtcgccaaga acattgtcgc gtccgggctg gcgaaaaagt
     4321 gcgaagttca ggtggcgtat gccatcggcg ttgccaagcc gctcagcatc aatgtcgaca
     4381 cctacggcac cggtaagctg gaagacaacc ggcttgaagc catcgtttct tccggggaaa
     4441 tcttcgactt ccgtccggcc aggctcatta aagacctggg actgctgacg ccgaaaggct
     4501 ggaactatcg cgataccgcc gcttatggac acttcggccg gccgcagttt ccctgggaaa
     4561 agacggacaa aatcgccgcg cttaaagccg ctgcgaaata gtttggtttt tgcggttgat
     4621 tcgccggagt tcacaaagta cattctgcgc ttctttgaag cacccatagc tcaattggat
     4681 agagcatctg actacgaatc agaaggtttc aggttcgact cctgatgggt gcaccaacta
     4741 aaaaacatca ataaaagccc tgttttgcag ggcttttttc tttttaccga aagtctccgg
     4801 aaggccggcg cgtttttgtc tttagttggc gtgacctgcc cccgtgtgtg acctgccccg
     4861 tgtgtggtga tggcccccct taaacgaaac cactcgcaga gttagtattc tgcgggtaaa
     4921 aacgaataa
//
LOCUS       DAOPZO010000131         4904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_21902_length_4904_cov_6.864302, whole genome shotgun sequence.
ACCESSION   DAOPZO010000131 DAOPZO010000000
VERSION     DAOPZO010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21902_length_4904_cov_6.864302"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..817
                     /locus_tag="PKI68_07905"
     CDS             134..817
                     /locus_tag="PKI68_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HNX53653.1"
                     /translation="MNGNTNKNRQVPAGNEGLIPKHGGFRNLKSFQLAQLIYDITVRF
                     CDKFVSPRSRTHDQMVQAARSGTQNIAEGSVDSATSKKIELKLTGIAKGSLEELRLDY
                     QDYLRQRSLPEWPPDHPALVRFKKLRCSTLQEFRAWVAEEAKKEKKSKTDKQGQARTD
                     TSGVKVGGSPCSPVFPSVCVANGALSLLNLCIYLLDRQLCAQAETFEKEGGFTERLYR
                     IRSERRRNS"
     gene            complement(822..1298)
                     /locus_tag="PKI68_07910"
     CDS             complement(822..1298)
                     /locus_tag="PKI68_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53654.1"
                     /translation="MFTPEHLHVALNHVALIGLAVAVIPLAVALIRKDRGALLSGFLI
                     VLLCGLSIPVVSETGDGAAERLVSGAAGIQIDSSAAAFLHEHEERADIASAFVYIAMG
                     LSAAGLLIMRIRPQHQYRIAALVLAVCIVCTALLVWTAEAGGQIRHPEFRPVPAAQ"
     gene            complement(1320..3098)
                     /locus_tag="PKI68_07915"
     CDS             complement(1320..3098)
                     /locus_tag="PKI68_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX53655.1"
                     /translation="MSAPEIKKRSLARRLLHYALPYWRGLLVSLILILAVSALINYLP
                     VLIKQLTDSCLLDTAAPAEVRLDRLSRISWIYLAIAVAGYALRYLQGMLTAWIGQRII
                     YDLRVAVFKKALRMQQAWFDRTPVGTLLTRVTSDIERLENFVTEGVVGTVADLFMLLG
                     IMGYMAVISPRLSVVLFALLPPLFALLVWVNRHLREANRQIRKRQAALNALLQEDITG
                     MPTIQLFNREPTAKADFDAHNIDLRAANYEEVRWFSVYFPVIETGQALAIAVILGAGG
                     WWLLHGGNALTLGVLIAFLAYIRDFFRPLGSLSDKAGAFQVAMASAERIFELMDTPET
                     IPDPAQPVPGERIGGAITFENVWFAYDKENWVIKNLSFSVEPGQVLAVVGATGAGKST
                     IINLIGRFYDVQRGRVTIGGTDVRDFSKTELRSRIGYVFQEPFLFAGSVADNISLLHP
                     GLTRDDIVRAAQSVNAHKFISALPAGYDTVLNERGGGLSLGQKQLIVMARTLAQNPEL
                     LFVLDEATANVDTATEILIQDALGKLMANRTSIVIAHRLSTIRYADRILVMRHGELID
                     QGTHDELMAHDGYYRQLYELLQHAPV"
     gene            complement(3095..4840)
                     /locus_tag="PKI68_07920"
     CDS             complement(3095..4840)
                     /locus_tag="PKI68_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX53656.1"
                     /translation="MKSDVKLSRYLAAHKAGIALTCLCVLVTVGVNLIMPYLMRLAID
                     GLTAGTLTQAGLLRYVLLYAGLAVFTVWFARQLRGIPLKLSHQIEYDVRRDLFAHLTR
                     LDAEFFRKERTGDLMTRMSSDLTIVTDAIGQGLLQGLRTGGALLFASIVMVWINPKLA
                     LLIFSLYLPIIAFFFLVLNVMRDRQKVLQEQVSEVSNFAQESFSGIRCIKGFALEKRR
                     TGFFELLNRGLIKKTLQLQTARQLLWPLMAFWFGIGTLLLLYVGGRQVIRGTVSVGVI
                     VQFLQYLLYMQWPLLSLSWTLSLMQRGKVSWRRILELFRREPKIADSAETDSGITALE
                     GSIEWRGISLEIDGAVRLHNINLTVPAGKTVGITGPTGSGKTLLVSLAARLMDVTEGK
                     LIVGGHPVRTIPLDVLRRHIGFAAQEPVLFSRTLEHNIAFGVTAPGQETIDWAADIAH
                     LNGDIAEFPEHYQTVLGERGITLSGGQRQRTSISRAVARRPQILILDDVLSAVDTQTE
                     AAIMGKLRPVMKDRTCLFVSHRVSTLRYTDEIIVIEDGRITQRGTHDELVKQSGYYSE
                     LNALQQLQQKLEGEP"
BASE COUNT         1043 a         1342 c         1599 g          920 t
ORIGIN      
        1 ttgaaaaaac ggtcatattt ttttccaacc cttgggaaat caatgacttc gccgcttaaa
       61 aattcctgcg cgcacggcag gaaaccggaa aactttatat tgctcttcgt ttatctttta
      121 ttaatgtcca gcaatgaacg ggaacacgaa taaaaacaga caggtgccgg caggaaacga
      181 agggctgatt ccgaagcatg gcgggttccg gaatctgaag agctttcagt tggcgcaact
      241 gatttacgat atcactgtgc ggttctgtga caagttcgtc agtccgcgaa gccggacgca
      301 cgatcagatg gttcaagctg cgcgcagcgg tactcagaat atcgccgaag gcagcgtcga
      361 ttcggcgacg tcaaaaaaga tcgaactcaa actgaccggc attgcaaaag gaagtttgga
      421 agaactccgc ctcgactatc aggactatct ccggcagcga agcctgccgg aatggccgcc
      481 ggatcatccg gcgctggtgc ggtttaaaaa gttgcgctgt tcgacgttgc aggaattccg
      541 ggcgtgggtg gctgaagagg cgaaaaagga gaaaaaaagc aaaacggaca aacaaggaca
      601 ggcacggaca gacacgagtg gtgtgaaagt cggtggcagt ccgtgttcgc ctgtgttccc
      661 gtccgtgtgt gtggccaacg gggcgctttc tcttttaaac ctttgcattt atctgctcga
      721 ccggcagctt tgtgcgcagg cggaaacgtt cgaaaaagaa ggcggcttca cagaacggct
      781 ttatcgaatc cgcagtgaac gccgccggaa cagttgacag tctattgcgc ggcgggaacc
      841 gggcggaatt ccggatggcg gatctgtccg ccggcttcgg cggtccagac gagcagggcg
      901 gtgcagacta tacaaaccgc cagcaccaga gcggcgatcc tatattgatg ttgcggccgg
      961 atgcgcatga tcagcagtcc ggcggcggaa agtcccatcg cgatgtaaac aaaagccgac
     1021 gcaatatcgg cccgttcttc gtgttcatgc agaaaggccg cggcggaact gtcgatttga
     1081 attccggctg cgccgctcac cagccgttcg gccgccccgt cgccggtttc tgaaacgacc
     1141 ggaatgctga ggccgcaaag caggacgatc aaaaagccgg aaagaagcgc gccccggtct
     1201 ttgcgaatca gggcaactgc cagcggaatg acggccacgg ccaggccgat cagcgcgacg
     1261 tgattcagtg cgacatgcag atgttcgggt gtaaacatga tgatttctcc ttttagttat
     1321 tatacggggg cgtgctgaag cagttcgtag agctggcggt agtaaccgtc gtgcgccatg
     1381 agttcgtcgt gtgttccctg gtcgatcagt tcgccgtggc gcatgacgag aatgcggtcg
     1441 gcgtagcgga tcgtggagag gcggtgggcg atgacgatgc tggtgcggtt ggccatcagt
     1501 ttgccgagcg cgtcctggat gagaatttcc gtggccgtat cgacgttggc ggtggcttcg
     1561 tcgaggacga agagcagttc gggattctgc gcgagcgtgc gggccatgac gatgagctgt
     1621 ttctgtccga gcgagagtcc gccgccgcgt tcgttgagaa cggtgtcgta gccggcgggc
     1681 agggcgctga taaatttatg cgcattgacg gactgcgcgg cgcggacgat gtcgtcgcgg
     1741 gtcaggccgg gatgcagcag ggaaatgtta tcggccacgg acccggcaaa gaggaacggc
     1801 tcctggaaaa catagccgat ccggctgcgc agctccgttt tgctgaagtc acgcacgtcg
     1861 gttccgccga tggtgacgcg tccgcgctgc acgtcgtaga accggccgat gaggttgatg
     1921 atggtgctct tgcccgcgcc ggtcgcgccg acgacggcga gaacctgtcc gggctcgacg
     1981 gaaaagctca ggtttttgat cacccagttt tctttgtcat aagcgaacca gacgttttcg
     2041 aaggtgattg cgccgccgat gcgttcgccg ggaacgggct gtgcgggatc gggaatggtt
     2101 tcgggcgtat ccatcagctc aaagatgcgc tcggcggagg ccatggcgac ctggaacgcg
     2161 ccggctttgt cggagagcga gccgagcggg cggaagaagt cgcggatgta ggcgaggaag
     2221 gcgatgagca cgccgagcgt cagcgcgttg ccgccgtgca aaagccacca gccgccggcg
     2281 ccgagaatga ccgcgatggc cagcgcctgg ccggtttcaa tgaccgggaa atagacgctg
     2341 aaccagcgga cttcctcgta gttcgcggcg cgcaggtcga tgttgtgtgc gtcaaaatca
     2401 gctttcgcgg tcggctcgcg gttgaagagc tggatggtgg gcatgccggt gatgtcttcc
     2461 tgaagcagcg cattcagcgc ggcctgccgc ttgcggatct gccggttcgc ctcgcgcaga
     2521 tggcggttga cccagacgag cagcgcgaag agcggcggaa gcaaagcgaa gagaacgacc
     2581 gaaagccgcg ggctgatgac ggccatatag cccataatgc cgagcagcat gaacaggtcg
     2641 gcgacggttc cgacgacacc ttctgtgacg aagttttcca ggcgctcgat gtcggaggtg
     2701 acgcgcgtca gaagcgttcc gacgggcgta cggtcgaacc aggcctgctg catgcgcagc
     2761 gcttttttga ataccgcgac gcgcaggtcg taaatgatcc gctggccgat ccacgcggtg
     2821 agcatgcctt gcaggtagcg cagcgcatag ccggcgacgg cgatggcgag gtaaatccag
     2881 ctgatgcgcg acagccggtc gagccgcact tccgcgggcg cggccgtgtc cagcaggcag
     2941 gagtcggtca gctgtttgat cagcaccggc aggtagttga ttaaagcgga aacggccaga
     3001 atcaaaatca gcgagaccag cagtccgcgc cagtagggca gcgcatagtg cagcaagcgg
     3061 cgtgcgagac tgcgtttttt gatttccggc gcgctcatgg ttcgccctcc agtttctgct
     3121 ggagctgctg cagggcattg agttcggaat agtagccgga ctgtttcaca agctcatcgt
     3181 gtgtgccgcg ctgggtgatg cggccgtctt caatgacgat gatttcgtcg gtgtagcgca
     3241 gggtcgagac gcggtggctg acaaacagac aggtgcggtc tttcatgacc ggccggagct
     3301 tgcccataat ggcggcttcc gtctgcgtat cgacggcgga gagcacgtcg tcaagaatca
     3361 gaatctgcgg tcggcgcgcc acggcgcggc tgatcgaggt gcgctggcgc tgtccgccgg
     3421 aaagcgtgat gccgcgctcg ccgagtacgg tttgataatg ctccggaaac tccgcgatat
     3481 cgccgttcag gtgcgcgata tccgccgccc agtcgatggt ttcctggccg ggcgcagtaa
     3541 cgccaaaggc gatattgtgt tccagcgtcc gcgaaaagag caccggctcc tgcgcggcga
     3601 aaccgatatg ccggcgtagc acatcgagcg gaatggtgcg gacggggtgg ccgccgacga
     3661 ttaactttcc ttcggtgaca tccatcaggc gggccgccag cgaaaccagc agggttttac
     3721 cgctgccggt cgggccggtg atgccgacgg tttttccggc gggaacggtc aggttgatgt
     3781 tgtgcaggcg aactgcgccg tcgatttcga gcgagatgcc gcgccactca atcgacccct
     3841 ccagcgcggt gatgccggag tcggtttcgg cagagtcagc aatcttcggt tcgcgccgga
     3901 aaagttccag aatccgccgc cagctcacct tgccgcgctg catcaggctc agcgtccagc
     3961 tcagcgagag cagcggccac tgcatataga gcagatactg gagaaactgc acgatcaccc
     4021 cgacgctgac ggtgccgcgg atcacctgcc ggccgccgac gtagagcaga agcagcgtgc
     4081 cgataccgaa ccagaacgcc atcagcggcc agaggagctg gcgggcggtt tgcagctgga
     4141 gcgttttttt aatcagaccg cggttcagca gctcaaaaaa tccggtgcgc cgcttttcga
     4201 gcgcaaaccc tttgatacag cggatgccgg aaaagctttc ctgcgcaaag ttggagacct
     4261 cggaaacctg ttcctgaaga accttctggc ggtcgcgcat cacgttgagc accaggaaga
     4321 agaaggcgat gatcggcagg tagagcgaaa aaatcagcag ggcgagcttc gggttgatcc
     4381 agaccatcac gatggaggcg aacagcagcg ccccgccggt ccgaagtccc tgaagcagtc
     4441 cctggccgat ggcgtcggtt acaatcgtca gatccgacga catgcgcgtc atcaggtcgc
     4501 cggtgcgctc cttgcggaaa aactccgcat cgagccgcgt cagatgggcg aagaggtcgc
     4561 ggcgcacgtc gtattcgatt tgatgcgaaa gcttgagcgg aattccgcgc aactgccggg
     4621 cgaaccagac ggtgaaaacc gccagaccgg catagaggag cacatagcgc agcaatccgg
     4681 cctgcgtcag cgtaccggcg gtcagtccgt cgatcgccag ccgcatgagg tagggcatga
     4741 tcaagttgac gccgaccgtc accagcacgc agaggcaggt cagcgcgatt ccggccttat
     4801 gcgccgcgag atagcggctt aatttgacgt ccgacttcat gacggctcaa aactaaagac
     4861 tctgttccgg aatacaagcg cagagccgca tgatgcggcg ggca
//
LOCUS       DAOPZO010000132         4899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_21933_length_4899_cov_8.569777, whole genome shotgun sequence.
ACCESSION   DAOPZO010000132 DAOPZO010000000
VERSION     DAOPZO010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21933_length_4899_cov_8.569777"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2774
                     /locus_tag="PKI68_07925"
     CDS             <1..2774
                     /locus_tag="PKI68_07925"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HNX53657.1"
                     /translation="STNQQYAPTWDPATGTLTASAISSTAEGAAVRGLGIDFTVQLKP
                     SANDQTVVLDEDTPQAITLTGSPLDGEALTYTVLTQPTNGVLSGTVPSLTYTPAANYN
                     GTDSFTFLVNNGLANSQTATVSIVVAPVDDAPVFVSTPLTLAAASKGAAYLQTLAGSV
                     TDPEGTTITYRKISGPAWLSIATDGTLTGTPGALEGGLNSWTVQATDETGLSSTGTVQ
                     IAVNANPAADVVSITGIGPTKIGTNLVATLSTSGGLFTDRSPAFPLAGGDPVSTLDSD
                     YYGVTVTNLSVGGVTNATLTFDLRLSLVTNATVKGLAIRTTSNYSFGYWIDSNGNSYS
                     RFFDSGAVLTFSVTNVTIDKGFEVPTVSFRADQLWGLYLNSGVVSTNLQYTPTWNPAT
                     GTLTASSFSSGVEGAAVRGLGIDFTFSTVPVAAPQSVTAYCYRSNAVTLAATPESSAF
                     TYTVLTQPAHGTLSGTAPNLFYTPSTNYSGADSFTFKASNGAVDSAPATVSITAQFVG
                     YPPAFTNGTLTRGIALIGTNYTGQTLSGSAADPENSALTYRKVSGPAWLQIASDGALS
                     GTPGIRDLGGNSWVVEVSDPTGLTGTATLSLTVRTTLFDVITMTGIEPTKTLTNLLAT
                     VSHSGQFFVDHRPTLSTNTTADYYGVTITNMVISGVSNVTMTFDLKLTTTPSALIGGW
                     NVRSNSYTTFGHWVSCKPGLQNNFFDSGAVLTFAVTNVTIDQGFAIAATSFRADPPWG
                     LYTNLATDVVSTNTRYAPTWDPATGTLTASTNYSSSEGAVVRGLGINFKVSLLPDYTA
                     WAEAHNLSGEDAYLQANPDGDRFNNLVEYALGSNPTNAANAGNRPIIRVTPQGVEFIY
                     RRRVDSLTRNRVNYTVQSTGSLMAPAWTNNFVETGSGTEADGMESVTNRAPGTGTTMF
                     GRLLINYTE"
     gene            3204..4490
                     /locus_tag="PKI68_07930"
     CDS             3204..4490
                     /locus_tag="PKI68_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53658.1"
                     /translation="MWFKKRWGLLVITVLSAFLLTGTAQAAESSPDELAQSSNTLFIL
                     IGAVMILAMHGGFAFLEVGSVRRKNQVNALNKIICEWGFSTIIYFLIGYPIARGVSFL
                     VPVETLAGNHGFAYVKFFLLLGFAACVPAIISGGVAERSKFWTNSLAGGIFVGLVYPL
                     VEGSLWGRFSNTLAGTTGWLAATFGAPFHDYAGSVVVHAVGGWLALPAIMLLGARLKR
                     YETNSIKVSSIPFLALGSWTLIVGWFGFNVMSAGSVEAISGLVAINSLMAMVGGTIAA
                     MFISRNDAGFVHNGALAGLVAVCAGSDIYHPVSALVVGSIAGAIFVFLFETETAKWQI
                     DDVLGVWPLHGACGAWGGIAAGIFGQKALGGVGGVSFFSQLIVTVAVILFCLAASTIV
                     YGLMKITVGIRLNPHEELIGSDLAVHRIEAHPEDAL"
     gene            4574..>4899
                     /locus_tag="PKI68_07935"
     CDS             4574..>4899
                     /locus_tag="PKI68_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNX53659.1"
                     /translation="MKMIIAIIQPEKLEDVKAALFNAEVHKMTVSRVRGCGQQMGFTE
                     HYRGQVRSVNLIEKVRLEIAVNDEFVDITIKAIIKAAKSGKIGDGKIFVMPLEQCIRI
                     RTDETGS"
BASE COUNT         1017 a         1455 c         1411 g         1016 t
ORIGIN      
        1 tttccaccaa ccagcaatat gctccgacat gggatccggc aaccggaacg ctgaccgcat
       61 cagccatctc ctcgacggca gaaggagccg cagtccgcgg actgggcatt gatttcacgg
      121 ttcaactgaa accttcggca aacgaccaga ccgttgttct ggatgaagat acgccgcagg
      181 cgatcacgct caccggcagc cctcttgatg gcgaagcact gacctataca gtactcactc
      241 agccgaccaa cggggtcttg tccggcacgg tgccgagtct gacctacacc ccggcggcga
      301 actataacgg aacggacagc ttcacctttc tggtgaataa cggcttggcc aacagccaga
      361 cggcgacggt gtcgattgtg gtggcaccgg tggatgacgc gccggttttt gtcagtacac
      421 cgctgaccct cgcggcggcc agcaagggcg cagcctatct tcagacgctg gcgggctccg
      481 tcaccgaccc ggaaggaacg acgatcactt accggaaaat cagcggcccg gcctggctgt
      541 cgatcgcgac agacggcacc ctgacaggca ctcccggagc cttggaaggc ggattgaaca
      601 gctggacggt tcaggcaacg gatgaaaccg gactgtcttc aaccggaaca gtgcaaattg
      661 cggtgaacgc caatccggca gcggacgtag tgtcgatcac cggcatcgga ccgaccaaaa
      721 tcggcaccaa tcttgtggcg acgctgagta cttccggcgg actctttacg gaccgtagtc
      781 cggccttccc gctcgcgggt ggcgatcccg tcagcacact ggacagcgac tactacgggg
      841 tcaccgtcac caatctgagc gtcggcggtg tgaccaatgc cacactgacg ttcgacctgc
      901 gcctgagcct ggtgacgaac gccacggtca aaggcctcgc aatacgcacc accagcaact
      961 atagcttcgg ctactggatt gattcgaacg gcaacagcta cagccggttc tttgacagcg
     1021 gtgccgtgct gaccttctcg gtcaccaacg tgacgatcga caagggcttc gaggttccga
     1081 ccgtgtcgtt ccgcgccgac cagttgtggg ggctctattt aaacagcggg gtcgtctcca
     1141 ccaacctgca gtatacgccg acctggaatc cggcaaccgg aacgctgacg gcatcgtcct
     1201 tctcgtcggg tgtagaagga gctgcagtcc gcgggctggg catcgacttt actttcagca
     1261 ccgtaccggt cgccgcgccg cagagcgtca cagcctactg ctatcgttcc aatgcggtca
     1321 cgctggcggc gactccggaa agcagtgcgt ttacgtacac cgtcctcaca cagcccgcac
     1381 atggtacgtt atccggcaca gcgcccaacc tgttctatac gccgtcaacg aactacagcg
     1441 gcgcggacag cttcaccttc aaggcgagca acggcgcggt tgacagcgcc ccggccacgg
     1501 tgtcgattac cgcccagttc gtcggctatc cgccggcgtt taccaacggc accctcacgc
     1561 gcggaatcgc gctgatcgga accaattata caggccaaac gctttccggc tcggcggccg
     1621 atcccgaaaa cagcgcgctg acctaccgca aggtaagcgg cccggcgtgg ctccagattg
     1681 catccgacgg cgcgctgtcc ggcacgccgg gtatccggga tctggggggc aactcctggg
     1741 ttgtcgaagt ctccgacccg accggcctga ccggcactgc caccttgagc ctgacggtcc
     1801 ggacaacgct cttcgacgtc attaccatga ccggcattga gccgacgaaa accttaacca
     1861 acctgctggc gaccgtcagc cattccggac agtttttcgt cgatcaccgg ccgaccctga
     1921 gcaccaacac caccgctgac tattacggcg tgaccatcac gaatatggtg atcagcggtg
     1981 tatcgaacgt cacgatgacg tttgatctga agctcacaac gacgccttcg gctcttatcg
     2041 gcgggtggaa cgtgcgctcg aacagctata cgacctttgg tcattgggtg tcgtgcaagc
     2101 cgggtctgca aaacaacttc ttcgacagcg gcgccgttct gacctttgcg gtgacgaatg
     2161 tgacgattga tcagggcttc gcgatcgccg ccacttcgtt ccgcgccgac ccgccgtggg
     2221 gcctttacac caatctggca accgatgtgg tgagcacgaa tacgcgctat gctccgacct
     2281 gggatcccgc gaccggaacg ttgacggcct cgacgaatta ttcgtccagt gaaggcgctg
     2341 tcgtgcgcgg tctgggcatc aactttaaag tcagcctgct tccggattac actgcatggg
     2401 cagaagcgca caacctgagc ggcgaagacg cctatctgca ggcgaatccg gacggcgacc
     2461 ggttcaacaa tctggtcgag tatgcgctgg gcagcaaccc gaccaatgcg gcgaacgccg
     2521 gaaaccggcc gataatacgt gtcacgccgc aaggtgttga gtttatctac cgccgccgtg
     2581 tcgattccct gacacgcaat cgcgtaaact ataccgtgca gtcgaccggc agcctgatgg
     2641 ctcctgcatg gaccaacaac ttcgtggaaa ccggcagcgg cacagaagct gacggtatgg
     2701 aatctgtaac caatcgtgcg ccgggcaccg gcaccacgat gttcgggcgg ctgctgatca
     2761 actataccga ataagcgtat cggatcgccg gatcaacctc agctgctgtc aggcggctga
     2821 ggttttttat tatcaatctc cagccggcga tctcaagctc tgcaatgaaa gcggccgcgc
     2881 agagcagctc cgtgaacagc atccattctt ccggactccg gcgccctgtt ccggatggaa
     2941 aaatacaaat ccggcgacaa tacaaaatgt tctccggcag atcctctttt gtcgtatttc
     3001 tgcgctaaag aatacaagtg tgtaattcgc tgccgctcat ccggcgcggt tgcctgcggc
     3061 gtttgataat tctaattcat taattacaaa tacttaagac cggtatctct tgccggcttt
     3121 tcctctcgag gcacaggacg tgcttttcct gcatctttaa tttgtgccgg aaacaggacg
     3181 ctgacaaacg gaggaaaaca attatgtggt ttaaaaaaag gtggggcttg ctcgtcatta
     3241 cggttttgtc ggcttttctg ctgaccggta ccgcgcaggc agcggagagt tcgcccgatg
     3301 agctggcgca gtcttccaat acgctgttca ttctgatcgg cgcggtgatg attctggcga
     3361 tgcacggcgg gttcgcgttt ctggaagtcg gttcggtccg ccggaaaaat caggtgaatg
     3421 cgctcaacaa gatcatctgt gaatggggct tttcgacgat catctacttt ctgatcggct
     3481 acccgattgc acgcggcgtt tcttttctgg taccggtcga aacgctggcg ggcaaccacg
     3541 gcttcgcgta tgtaaaattt ttcctgctgc tcggctttgc ggcctgcgtt ccggcgatca
     3601 tttccggcgg tgtggcggag cgttcaaagt tctggaccaa ctcgctggcc ggcggaattt
     3661 ttgtcggtct ggtttatccg ctggtggaag gttctctctg gggccgcttc tcaaacacgc
     3721 tggcgggcac aaccggctgg ctggcggcga cgttcggtgc gccgttccat gactacgcgg
     3781 gttccgtggt ggtacacgcg gtcggaggct ggctggcgct gccggcaatt atgctgctcg
     3841 gcgcgcgcct gaagcgctac gaaaccaact ccatcaaagt cagcagcata ccgtttctcg
     3901 cgctgggcag ctggacgctg atcgtcggct ggttcggctt taacgtaatg agcgcgggct
     3961 ccgtcgaagc aatctccggg ctggtggcga tcaactcgct gatggcgatg gtcggcggca
     4021 cgattgccgc catgtttatt tccagaaacg acgccggctt cgtgcataac ggcgcgctgg
     4081 ccgggctggt ggcggtttgc gccgggtcgg acatttatca tccggtgagc gcattggtcg
     4141 tcggctcaat cgccggcgcg atttttgtgt tcctctttga gacggaaacg gccaagtggc
     4201 agatcgacga tgtactcggc gtctggccgc tgcacggcgc ctgcggtgca tggggcggca
     4261 ttgccgccgg tattttcggc cagaaagcgc tcggcggtgt cggcggcgtc tcgttctttt
     4321 ctcagctgat cgtaacggtg gcggtgatac ttttctgtct ggcggccagc acgatcgtgt
     4381 acggcctgat gaaaatcacc gtcggcatcc ggctgaatcc gcacgaagag ctgatcggct
     4441 ccgacctggc ggtacaccgg attgaggctc atcctgaaga cgcgttgtaa ttcagttatc
     4501 agaaaacagt tatcagttaa aagttcccga taacaaataa ccagtaacga ataactcgaa
     4561 aggtactttc gccatgaaaa tgattattgc cattatccag cctgaaaaac tcgaagacgt
     4621 caaagcggcg ctgttcaacg ccgaggtgca taagatgacc gtcagccgcg tgcgcggctg
     4681 cggccagcag atgggattca ccgagcacta ccgcggacag gtccgctccg tcaacctgat
     4741 cgaaaaggtg cggctggaaa tcgcggtgaa cgacgagttt gtcgacatca ccatcaaagc
     4801 gattatcaaa gccgccaaga gcgggaaaat cggtgacggt aaaatattcg tcatgccgct
     4861 ggagcagtgt atccgcatcc gcaccgacga aaccggctc
//
LOCUS       DAOPZO010000133         4799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_22617_length_4799_cov_9.430017, whole genome shotgun sequence.
ACCESSION   DAOPZO010000133 DAOPZO010000000
VERSION     DAOPZO010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22617_length_4799_cov_9.430017"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..791)
                     /locus_tag="PKI68_07940"
     CDS             complement(18..791)
                     /locus_tag="PKI68_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNX53660.1"
                     /translation="MQTVTSKDNPKLKAVRALLRSKKDRQEASAFVIEGVRALSALAG
                     GPALPQYRLKEVWVSSDAPELAKDFSAPVYAVAHDVMEQLSDCRTSQGILGVVDYVPV
                     PLEINPETGNYLLVDSLTDPGNLGTLIRSAVAFGFAGVFLHGETVEVFNPKTVRATMG
                     SLPFINCRETDETVFKTLEKAGYDLISTVVHGGENLHQMKFGPKNVLVIGNEAHGVSE
                     AVQNCATRKLSIAMAGDVESLNAAVAGSICMFAMSGRRN"
     gene            complement(791..1513)
                     /locus_tag="PKI68_07945"
     CDS             complement(791..1513)
                     /locus_tag="PKI68_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNX53661.1"
                     /translation="MFGTERSERGFCRQKQRPKRSAKVRILTVAILAAGTLFAGLPDA
                     ATPVTNLTFTAFDTETTGFSPKNDRIVEIGAVRFRGDGEILAVTNWLINPGMPMPFYA
                     TQANGITTEMVSGAPVFSAVWPEFAAFCQDSILLAHNANFDAGFLRAELTRAGVEPPD
                     LTVGDTLPLFRCWFPQAKSYALEPLSVYLGVQGGNYHRAEADSFHIINIFNAGMKLRP
                     DMRFQQLERDAGGFIRLNGRRN"
     gene            1601..>2466
                     /locus_tag="PKI68_07950"
     CDS             1601..>2466
                     /locus_tag="PKI68_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNX53662.1"
                     /translation="MKLASDIRTLSPQLQVSENALLRNYTTFQLGGPCPILIEGATAA
                     QLPAIIKLLNKAGQPFLVIGQGANLIVSDKGIKQAVIRFCSEKPVITASGNRVTVSGD
                     TLLDALALFTIENEVGDLSYCTGIPGTVGGGIAGNAGAFGRQMGDHLVSVELLGLDGT
                     VRTVLHDELEFSYRHSKLKETGEIVLSATFELPAAEKSIMQAERDRILQFRKDHHPDW
                     HNEPCAGSVFRNIEPTSAVERRKAAGFFLQEAGAHLFRVGGARLYEKHANIIVADPGC
                     TAQDVWELCGKMA"
     assembly_gap    2467..2566
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2567..4032)
                     /locus_tag="PKI68_07955"
     CDS             complement(<2567..4032)
                     /locus_tag="PKI68_07955"
                     /inference="COORDINATES: protein motif:HMM:NF015177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HNX53663.1"
                     /translation="MRKILIVMTLSVFTSLSFLQAGESDDWRASVPVPVFEKEPGYVE
                     LYWKAWEIAHQKIKTQPGLPQSPYIDEAFWDDTIWIWDTCFMALFCKYAPDEFPGVES
                     LNNFYVPLHDKRYNDGAYPLNIQHPDNPPLFAWVEYDNYTITGDRNHVIELLTKTKYL
                     QKHFEWFDQMEPGWKMKSSAPQHKQSAPTALKKVKNGYLWDGVQSGMDNTPRKRGGLW
                     IDAISQQALSALYISRLADASGCTEISAEWKEKYQELKKTINTLYWNAEDGIYYDLNP
                     ETYEPLKVKTPAAYWPMLAEICTPEQAERMREHIQNPAVFGGERPWVTVARDDPAFTL
                     PDGDYWRGGIWLPTAYMGTKALEKYGFFKEADAAAENLLAHMFRTYKTYEPHTIWECY
                     SPSRDTPANHGRRRVRPDFCGWSALGPISLFIENVMGFHQINALERKVDWRLYQTTKH
                     GLKHLRFGGIDTDIIYNGKNAVVVTSNSEYTLVINGVAR"
     gene            complement(4179..>4799)
                     /locus_tag="PKI68_07960"
     CDS             complement(4179..>4799)
                     /locus_tag="PKI68_07960"
                     /inference="COORDINATES: protein motif:HMM:NF039902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53664.1"
                     /translation="NYLALLGWSPGDEREVMTRDEMIAAFDLSTVRSSPAQMDLRKLL
                     WMNGEYIRAMRPVDFATLCREELRTMNLEIGENAADIFALMQERIKIRTDIIPAMTFF
                     CTEDYEYDEKAVEKKLAKPEVFQTLEKIAGILESLPQFDAETVNKALHDFVEQGGLKF
                     GDVMPPLRIAVSGQQSGPDLAPVLAILGKDNVLARIRKTLARFSRF"
BASE COUNT         1003 a         1380 c         1261 g         1055 t
ORIGIN      
        1 taaatattca gcaaacttca attccgccgc ccgctcatgg cgaacataca gatcgacccg
       61 gcaacagcgg cgttcagcga ttcaacgtct ccggccatcg caatcgaaag cttccgcgtt
      121 gcgcagttct gaaccgcttc agaaacgccg tgcgcttcat tgccgatcac cagcacattc
      181 ttcgggccga acttcatctg atgcagattc tcgccgccgt gaacgacggt cgaaatcaga
      241 tcgtatccgg ctttctccag ggttttaaaa accgtttcat ccgtttcccg gcaattgatg
      301 aacggcagcg agcccatcgt tgcccggacg gttttcggat taaacacttc gaccgtttcg
      361 ccatgcagaa aaacaccggc aaagccgaac gcaaccgccg agcggatcag cgtaccgagg
      421 ttgcccggat ccgtcagact gtcgaccaga agatagtttc ctgtctccgg attgatctcc
      481 aaaggtaccg gcacatagtc caccacgccg agaataccct gcgaagtccg gcagtcggaa
      541 agctgctcca tcacatcgtg cgcaacggca tagacgggcg ctgaaaaatc ttttgccagt
      601 tcgggcgcat cagaggaaac ccagacttcc ttgagccggt actgcggcag cgccggaccg
      661 cccgccagag cggaaagcgc gcggactcct tcaatcacga acgcggaagc ttcctgccgg
      721 tctttcttcg aacgcagcag tgcgcgcacc gccttcagtt taggattatc ttttgaggta
      781 accgtctgca tcagttccgc cttccgttca gccggataaa accgcccgcg tcgcgctcca
      841 gctgctggaa ccgcatatcc ggacgaagct tcatgcccgc attaaaaata ttgatgatgt
      901 gaaatgaatc ggcttcggca cggtgataat ttccgccctg cacgccgaga tataccgaaa
      961 gcggctcaag cgcgtaactc ttcgcctgcg gaaaccagca gcggaaaagc ggcagcgtat
     1021 cgccgaccgt cagatcgggc ggttccactc cggcccgcgt cagctcggcc cgcagaaacc
     1081 cggcgtcgaa attcgcattg tgcgccagca gaatactgtc ctgacagaac gcggcaaact
     1141 ccggccagac tgcactgaac acaggcgcgc cggaaaccat ttcggtcgta atcccgtttg
     1201 cctgagtcgc ataaaaaggc atcggcatac cgggattaat cagccagttg gtaacggcca
     1261 gaatttcgcc gtcgccgcga aaccggaccg cgccgatttc aacgatccgg tcatttttcg
     1321 ggctgaagcc ggtcgtttcc gtgtcgaacg ccgtaaacgt cagattggtt accggcgttg
     1381 cggcatccgg aagtccggca aaaagcgttc cggcagccag aattgcgaca gttaaaatcc
     1441 gtacttttgc cgatctcttc ggtcgctgct tttgcctgca aaagcctctt tcacttcgtt
     1501 cagttccaaa cattggaacc gatagtaatg tatcggcact taatctgaga gcggaaattt
     1561 ttgtgttctt tgtgctcctt cgcggctaat gtcttcgacc atgaaattgg catccgacat
     1621 ccgaaccctt tccccgcaac tgcaggtcag tgaaaacgcc ttgctgcgca attacaccac
     1681 ctttcagctg ggcggcccct gcccgatcct gatcgaaggc gcaaccgccg cgcagcttcc
     1741 cgccattatc aaactgctca acaaagccgg acagccgttt ctcgtcatcg gccagggggc
     1801 taacctcatt gtttccgaca aaggaatcaa gcaggccgtc atccgcttct gctcagaaaa
     1861 gcccgtgatt acagcctccg gcaaccgcgt caccgtttcc ggcgacaccc tgctcgacgc
     1921 tctggcgctc ttcaccatcg aaaacgaagt cggcgatctg tcgtactgca ccgggattcc
     1981 cggaaccgtc ggcggcggta tcgccggaaa cgccggcgcc ttcggacggc agatgggcga
     2041 ccacctcgtc tcggttgaac tgctcggact ggacggaacc gtccgcaccg tcctgcacga
     2101 tgaactcgaa ttttcctacc gccactccaa actcaaagaa accggcgaga tcgttctctc
     2161 cgccaccttt gaacttccgg cagcggaaaa atctatcatg caggccgagc gcgaccggat
     2221 tctgcagttc cgcaaagacc atcatcccga ctggcacaac gaaccgtgcg ccggaagcgt
     2281 cttccgcaac atcgagccga cctccgccgt cgaacggcgc aaagcggccg gtttcttttt
     2341 acaggaagca ggcgctcatc tgttccgcgt cggcggcgca cggctctacg aaaagcacgc
     2401 caacatcatc gtcgccgatc ccggctgcac cgcgcaggac gtctgggagc tttgcgggaa
     2461 aatggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncggg caacgccatt
     2581 gattacgagg gtatattccg aattgcttgt gacgaccacg gcatttttac cgttgtagat
     2641 aatgtcggtg tcgatgcccc cgaaccgtag atgtttcagc ccgtgctttg tggtctggta
     2701 aagtcgccag tcgacctttc tctccagcgc attgatctgg tgaaagccca tgacgttctc
     2761 aatgaataag gagatgggcc ccagcgccga ccacccgcag aagtccggtc gaacccggcg
     2821 ccggccgtgg ttggccggtg tatcgcggga ggggctgtaa cattcccaaa tggtgtgcgg
     2881 ctcgtaggtt ttatacgttc ggaacatgtg cgccagaagg ttctcggcgg cagcgtcggc
     2941 ttctttgaaa aacccgtatt tttcgagggc tttcgtgccc atataagccg tcggcagcca
     3001 aattccaccg cgccagtaat ctccgtcggg gagcgtaaat gccggatcgt ctctggcgac
     3061 cgtcacccag gggcgttctc cgccgaagac agcgggattc tggatgtgct cacgcattct
     3121 ctccgcctgt tcgggcgtgc aaatctctgc cagcatcggc cagtaggcgg cgggggtctt
     3181 cactttgaga ggctcgtagg tttcgggatt caaatcgtaa taaatgccat cttccgcatt
     3241 ccaatacagc gtgttgatcg tctttttcag ctcctggtac ttttccttcc attcggcgga
     3301 aatctccgtg catccactgg catcggccag tcgactgatg tagagcgcgg agagcgcctg
     3361 ctgagaaatg gcgtcaatcc agagaccgcc gcgtttgcgc ggcgtattgt ccatgccgct
     3421 ctgaacaccg tcccaaagat agccgttttt taccttcttg agggctgtcg gtgcgctttg
     3481 tttgtgctgc ggagccgacg acttcatttt ccagccgggc tccatctggt cgaaccactc
     3541 gaaatgcttt tggaggtact ttgttttcgt cagcagttcg atgacatggt tgcggtcgcc
     3601 ggtaatggtg tagttgtcat actcgaccca ggcaaacagc ggcggattat ccggatgctg
     3661 aatattcagg ggataagccc cgtcgttata gcgtttgtca tgaagcggaa catagaagtt
     3721 attcaggctt tccacgccgg ggaactcgtc aggagcatac ttgcagaaca gcgccatgaa
     3781 gcaggtgtcc cagatccaga tcgtatcgtc ccagaacgct tcgtcgatat agggcgactg
     3841 tgggagaccc ggctgcgttt tgattttctg atgtgcaatc tcccacgctt tccaatacag
     3901 ctcaacatag cccggctctt tctcaaatac cggaacgggc accgatgctc tccagtcgtc
     3961 actctctcca gcctgcaaaa acgagaggga tgtaaagaca ctcagggtca tgacaattaa
     4021 aattttacgc ataatgtccc ttttttctgg ggtcggaatc taaagagctt ctgaggcaat
     4081 ctccagccgg catatcggct ccgtaaggat cgttgtcttt atgcggtgtg cgtgctctcc
     4141 atgacaaaga ctccttgcat aaagactgct tcagggcgtt aaaaccggct gaaacgcgcc
     4201 agcgttttgc ggattcgggc gaggacatta tctttgccaa ggatcgccag aaccggcgca
     4261 aggtcggggc cggactgctg gccggaaacg gcgatacgca gcggcggcat gacatcgccg
     4321 aatttgagtc cgccctgttc gacgaagtcg tgcagggctt tattgacggt ttcggcgtcg
     4381 aactgcggca ggctttcaag aattccggca attttttcca gcgtctggaa aacttccggc
     4441 ttcgccagtt tcttctcaac ggctttttcg tcgtattcgt aatcttccgt acagaagaag
     4501 gtcatggccg gaatgatgtc ggtacggatt tttatacgct cctgcatcag cgcaaaaatg
     4561 tctgcggcgt tttcgccgat ttcaagattc atggttcgga gctcttcgcg gcacagggtg
     4621 gcgaagtcga ccggccgcat ggcgcggata tattcgccgt tcatccagag cagtttccgc
     4681 agatccatct gggcgggcga tgagcggacg gtggagaggt cgaaggccgc gatcatttcg
     4741 tcgcgggtca tcacttcgcg ttcgtcgccg ggcgaccagc cgagcagtgc gagatagtt
//
LOCUS       DAOPZO010000134         4714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_23213_length_4714_cov_9.460399, whole genome shotgun sequence.
ACCESSION   DAOPZO010000134 DAOPZO010000000
VERSION     DAOPZO010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23213_length_4714_cov_9.460399"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2565
                     /locus_tag="PKI68_07965"
     CDS             <1..2565
                     /locus_tag="PKI68_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNX53665.1"
                     /translation="IPDLDHTIRGVLAGDNIVWQVDDIAEYQAFVLPFAQAAKDNGRV
                     LVYFRFARHEPLLPSFMAAEVHELDPQIGFEGFIARIHEVIRRVGKGAFYVFDCLSCL
                     AVDWYSDQMLGNFFMLTCPYLFDLETVTYFALFRNYHTQRAIGPISRTTQLFLDVYSH
                     KGNLYLRPMKTQHRHSPTINMLHEWRKADDTFHAVSSSNVISEILTSARWSGLSADSS
                     GDFWETAFVRAREMIKAGTYRPDIPERGKILYEQLARMIISRDEGMQRLIARNLTLED
                     ILDVRKRMIGTGLIGGKTVGMLLARAILKRADPRYVELLEEHDSFFIGSDVFYTFLVR
                     NGIWKVRQNQRDPERFLEGAEEARHMIIRGDFPDYILQQLDEMLDYFGPSPFIVRSSS
                     LLEDNYGNSYAGKYDSVFCANQGPRERRRQDLLAAVKTIYASSMSERALNYRAHRGAL
                     DKDEQMALLVMRVSGASYGHYFYPQAAGVAFSFNPYAWSPEIDPKAGVMRLVFGLGTR
                     AVDRSDDDHTRIVALNAPDKRPEGNFEATLQYTQRRVDCLDLESNQPVSELFSDLIKT
                     PPEDLPLELFAAADFVSLPDGRRVERRMLTFDKLLKETPFVQDMRDILRVLHEAYDYP
                     VDIEFTMNFVDEKNYRINLVQCRPLQVQGIGAVKLPAIKVPEKDRIIEARGAVVGQSR
                     LIPVDRLIYVVPALYGKLPQQERYAVARLIGEINRTSPADSTTMLLGPGRWGTSSPEL
                     GIPVSFSDINQVNVLCEIVAMHEHLVPDVSLGTHFLNELVENNMLYLALFPKQGNNFL
                     NEEFFQQSRNRLLDLVPSAEKWADTVHVIEAHDLPGGSGVNLWANAIEQKVICYLK"
     gene            complement(2869..3216)
                     /locus_tag="PKI68_07970"
     CDS             complement(2869..3216)
                     /locus_tag="PKI68_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HNX53666.1"
                     /translation="MSEIPACPKCKSEYAYEDGAMFVCPDCGHEWTPGSGEEAETPAT
                     EKVIKDANGNVLQDGDTVSVIKDLKIKGSSLVVKVGTKVKNIRLVDGDHDIDCKIPGI
                     GAMGLKSEFVKKV"
     gene            3622..3939
                     /locus_tag="PKI68_07975"
     CDS             3622..3939
                     /locus_tag="PKI68_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53667.1"
                     /translation="MAIREVLEEELENSLRMEQRYSEELGRLPQGSLVQKRIKGHAYY
                     YLVLRDEAGQVKLIYKGKVEPEEIARYKEIKQQRAQYRNLRAKVRGQIRFLKRALNAK
                     AAV"
     gene            3920..4612
                     /locus_tag="PKI68_07980"
     CDS             3920..4612
                     /locus_tag="PKI68_07980"
                     /inference="COORDINATES: protein motif:HMM:NF023701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HNX53668.1"
                     /translation="MQKPQYDLCCEVLRRLRAQRVLPYLVLTGSWCLLLYRQYFERDE
                     LFSSLRTRDMDFLVPIPLTLRDKINLPELFKDLGFLTEYKGTAGYMQLIHPEVMLEFL
                     VPQRGRETDRPFDLPQLGLNAQRLRYMDIALMKPIKLMFEDVPIRVPHPACFALHKLL
                     VAPRRKEAFKQARDIEIAVELLNLLFDRNDQPVLKEILNRFPASWRKTALKVLSGAGQ
                     ETLHDRLAELGP"
BASE COUNT         1013 a         1306 c         1336 g         1059 t
ORIGIN      
        1 attccggatc tggatcacac gattcgcggc gtgctggcgg gcgataatat tgtctggcag
       61 gtggatgaca tcgctgagta tcaggcgttc gtgctgccgt tcgctcaggc ggcgaaggat
      121 aacgggcgcg tactggttta tttccgtttc gcgcgccacg agccgctgct gccgtctttc
      181 atggctgcgg aagttcatga acttgatccg cagatcggct tcgaaggttt tatcgcccgc
      241 attcatgaag tgatccgccg cgtcgggaag ggggcgttct atgttttcga ctgcctgtcc
      301 tgtctggcgg tggactggta ttccgatcag atgctcggca attttttcat gctgacctgt
      361 ccgtatctct tcgaccttga aacggtcacc tacttcgcgc tgttccgcaa ctatcacacg
      421 cagcgcgcca tcggtccgat cagccgcaca acccagcttt ttctcgatgt ctattcccac
      481 aaaggcaatc tgtatctgcg gccgatgaag acacagcacc gccactcgcc gacgatcaac
      541 atgcttcacg aatggcggaa agcggacgac accttccatg ctgtcagctc cagtaatgtg
      601 atttctgaaa ttctcacctc ggcccgctgg tcgggactga gcgcggacag cagcggcgat
      661 ttctgggaaa cggcatttgt ccgcgcccgc gaaatgatca aagcgggaac ctaccggccg
      721 gatattcccg aacgcgggaa aatcctctac gagcagttgg cgcggatgat catttcgcgc
      781 gacgaaggga tgcaacggct gattgcgcgc aatctgacac tcgaagatat tctcgacgtg
      841 cgcaagcgga tgatcggtac cgggctgatc ggcggtaaaa ccgtcgggat gctgctggcc
      901 cgcgcgattc tcaaacgcgc cgatccgcgt tatgtggaac tgctcgaaga acacgattcg
      961 tttttcatcg gctcggacgt gttctacacc ttcctcgtgc gcaacggcat ctggaaagtg
     1021 cgccagaacc agcgcgaccc ggagcggttt ctcgaaggag ccgaagaagc ccgccatatg
     1081 attatccgcg gcgatttccc cgactacatt ctccagcagc tggacgaaat gctcgactac
     1141 ttcggcccgt cgccgttcat cgtgcgctcg tcgtcgctgc ttgaagacaa ctacggcaac
     1201 tcctacgccg gaaaatatga cagcgtcttc tgcgccaacc agggaccgcg cgaacgccgc
     1261 cgtcaggatc tgctcgccgc cgtgaaaacc atttacgcca gctccatgag cgagcgggcg
     1321 ctgaactacc gcgcccaccg cggcgcgctc gacaaggacg agcagatggc gctgctggtc
     1381 atgcgggttt ccggcgcttc gtacggacac tacttttatc cgcaggcggc gggcgttgcc
     1441 ttctcgttca atccctacgc gtggagcccg gaaatcgatc cgaaggccgg tgttatgcgg
     1501 ctggtcttcg gactcggcac gcgcgccgtg gaccgctccg acgacgatca cacccgaatc
     1561 gtcgcgctga acgcgccgga taaacggccg gaaggaaact ttgaagcgac gctgcaatat
     1621 acgcagcgcc gcgtggactg cctcgacctc gaatccaatc agccggtttc cgagctgttc
     1681 agcgatctca tcaaaactcc gccggaagat ctgccgctgg aactgtttgc cgccgccgac
     1741 tttgtcagcc tgcccgatgg ccggcgggtt gagcgccgga tgcttacgtt cgataaactg
     1801 ctcaaggaaa cgcccttcgt tcaggatatg cgcgacatcc tccgtgttct gcatgaagcc
     1861 tacgattatc cggtggacat cgaattcacc atgaacttcg tggatgaaaa aaactaccgc
     1921 atcaacctcg tccagtgccg cccgcttcag gtgcagggca tcggcgcggt gaaacttccc
     1981 gccatcaaag ttccggaaaa agaccgtatc atcgaagcgc gcggcgcggt cgtcgggcag
     2041 agccgtctga ttccggtgga tcgtctgatc tatgttgtgc ctgcacttta cggaaaactt
     2101 ccgcagcagg aacgctacgc cgtcgcccgg ctgatcggcg aaatcaaccg cacctcgccg
     2161 gcggacagca ctaccatgct gctcggcccc ggacgctggg gcaccagcag tcccgaactt
     2221 ggaattcccg tcagcttcag cgacatcaat caggttaatg tcctgtgtga aattgtcgcc
     2281 atgcacgaac atctggttcc cgacgtatcg ctcggcacgc acttcctcaa cgagctggtc
     2341 gaaaacaata tgctctatct ggcgctcttc ccgaagcagg ggaacaactt cctcaacgaa
     2401 gagttttttc agcagagccg caaccggttg ctcgatctgg ttccgtcagc ggaaaaatgg
     2461 gccgacaccg tgcacgtcat cgaagcgcac gacctgcccg gcggcagcgg cgtcaacctc
     2521 tgggccaacg ccatcgaaca gaaagttatc tgctacctca agtgatagga gcagaaggcc
     2581 ttggacttta ggccatagac tttgggttcc gagccttggg aaaatcccct ccacagggga
     2641 gaggtggccg ggacggcggg gtgggttgaa gttccaatgc gtgggaatct gacaccattt
     2701 gcggatgata ttcagtattt gatcaaggca ggctggacgc gaagcgaggc tttcggaacg
     2761 aaagcaatga ccgcagggaa tggcaaccct gcggtacgta tcgcaggctt ccagcctgcc
     2821 ttgtctttta aaccgaatgt catacggttt tggtatgagt accgacgatt aaactttttt
     2881 gacgaactcg gacttcagcc ccatcgcgcc gatgccgggg attttacagt cgatgtcatg
     2941 gtcgccatcc acgaggcgga tgtttttgac tttggttccc actttgacga ccagtgacga
     3001 acctttgatt ttcaaatctt tgatgaccga aacagtgtct ccatcctgaa gcacgttccc
     3061 gttggcgtct ttgatgactt tttccgtcgc gggcgtttcc gcctcttcgc cgctgccggg
     3121 cgtccattca tgaccgcagt cggggcagac gaacatcgcg ccgtcttcat aggcatattc
     3181 agatttacac ttcgggcagg cagggatttc gctcatcagt tctccttttc aaaggcgaag
     3241 agttaaccac gaacggacac gccgttccag agatgcttag gggaaattat tcctcgcaaa
     3301 gacacagagg gcgcagagaa attttaacct ctgaaaaatt actagagtac ggggcgaacc
     3361 ccagccccag ctttttgtta cttagcgacg tgtgccgatt ttaaacccgt ctcatatttc
     3421 ttcgggagga ggtttacgtt ggtcggaggg atgatcagcg ccggtattcg cccggacgat
     3481 gcgcgactgt ccgcggaggg gaagatgttg tcatacaagc atgggtatat aatttttgta
     3541 tggtaaccgc ttgacgggcg gaacgggttg gcatacaaac acttcagatt tgatatttgt
     3601 atgacaaccg tgaggtgtga gatggcgatt cgcgaggtgc tggaagagga gctggaaaat
     3661 tcactgcgaa tggagcagcg gtattctgag gagctgggca ggctgccgca gggcagtctg
     3721 gtgcagaagc gcatcaaggg ccatgcctat tattacctcg tgttgcgcga tgaagccggg
     3781 caggtgaagc tgatttacaa aggcaaggtg gagccggaag aaattgcccg ctacaaagag
     3841 atcaagcagc aacgggcgca gtaccgcaat ctgcgcgcaa aggttcgcgg acagatccgt
     3901 tttttgaaga gggcgctcaa tgcaaaagcc gcagtatgat ttgtgctgtg aagtgcttcg
     3961 gcggttgcgt gcgcaacggg tgcttccgta tcttgtgctg accggaagct ggtgtctgtt
     4021 gctgtaccgt cagtattttg aacgggatga gctgttttcc agcctgcgta cccgtgatat
     4081 ggattttctg gttcccattc cgcttacact gcgcgataaa atcaatcttc ccgaactgtt
     4141 caaggatttg ggctttctga ccgaatacaa aggcaccgcc ggatacatgc agcttattca
     4201 tcccgaagtg atgctggagt ttctggtgcc acagcgcgga cgcgaaacag accggccgtt
     4261 tgatctgcct cagttgggtc tgaatgcaca acgcctccgc tacatggata tcgcgctgat
     4321 gaagccaatc aagctgatgt ttgaagatgt tcccatccgg gtaccgcatc cggcctgttt
     4381 cgcattgcac aaactgctgg tggcgcctcg tcgcaaagag gcgtttaagc aggcgcgcga
     4441 tattgagatt gccgttgaac tgctcaatct gctgtttgac cggaatgatc agccggttct
     4501 gaaagagatt ctcaaccggt ttcccgcatc ctggcgcaag acggctctaa aggtgctgag
     4561 tggagccgga caagaaactt tgcatgaccg tttggcagaa ctgggcccat aatttccaat
     4621 gcatggaaac tccgggttga aaagcagatc cgcttgctgg caagcggatc tgccggatgc
     4681 ttcttaccgg cgcggaagtt ccgcggtgcc gcgg
//
LOCUS       DAOPZO010000135         4634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_23775_length_4634_cov_11.601660, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000135 DAOPZO010000000
VERSION     DAOPZO010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23775_length_4634_cov_11.601660"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="PKI68_07985"
     CDS             complement(<1..1218)
                     /locus_tag="PKI68_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53669.1"
                     /translation="MKHLKIRNRFFAGGAALLLIVSAGVVAAPPEGRAQNVPAVGKPQ
                     LAGTWCPACKRYIDCPNGKMPSACPCGYSFVQKAKPRPQLTPGMPQPGRSPDEPQDDR
                     LYYDGTVTAGGLRTRHFESNGLAARAEAWGKEMKQVNEQSLKKQQTQAQAFENDKNAL
                     LNSPGSGEARQQLRNVFADEAAWDSPSKSGGDPNGVDLRHLGDSPGTPALLRKPEPKQ
                     PSEYDFQKESAAAKESPKAGDGTEKDLILGSTLARAIATKDATVTLKPYQPEEVAKIT
                     AENGWVIAARDLDGKAAPYVVNKLSGRDDNPYGMHWQILLPNGESVGYFNGTAAGQDG
                     KTLPNIKSETGAQYKGVVASGKNGAVMTEAVKNVKARWEEKYLSDPEGQSYNAGAHNC
                     QDFTGDVLREYQRL"
     gene            complement(1239..3242)
                     /locus_tag="PKI68_07990"
     CDS             complement(1239..3242)
                     /locus_tag="PKI68_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX53670.1"
                     /translation="MKKLTLLTAGSVTACLLAVSSAVCWAQTPQENSGAAAAQRAAEI
                     AAAKAAAARDPQNWRVFNDLGAVYYRQAQYDQAVAAFQQALSLHSITTIIEAEKKQAD
                     EAAAQRAAMNAKREADIQRAKDLQSKQEMNELFGLVSGVAGMNGNMQAQMMASTLQTV
                     NNQINSVPNSVPPLSPEMKMESSLKAKSEVSGLYISLGFAYFGKKDYQQAILSFDNAL
                     QLDPSRTEVLKTSAEALYFLCKYDECITTFAKYHAIAPVEPASLLRLSDAYRALGMHA
                     ESDKAFAAFLAGQERYAADCCKLLEIGSVCLSHWRYEEALSFLTKARQVAAGSPDQSR
                     RFIEAQRARFQMTNAISLLLAEAQFNLRQTAEATALLREAVAQDGSNSKAWYMLAQCH
                     DQAGESAAAQEAYGKALAAFGAAKTKAPDYIQVCRAAAGEGDEAVKALETQLAGVPLT
                     PGGGANQWCVLALAFEKAGRIPEAMEILSRCCEVSPAYTRARLALTRLEQQAAAERKR
                     ILAEADAALAAGDRNQALAKLADVYRLTAAGKEKEAVRKTLLKTAAGLEPAPAMNNSA
                     QDHYLRGSVALKTAKNPLDLSRALSEFQWAVFYSPWVGDLYFNTSAVKKLQNQTAAAV
                     GDLKLYLAAKPDAANVGEILNRLYEFDYQQEQKLRELAAVAAF"
     gene            complement(3259..3909)
                     /locus_tag="PKI68_07995"
     CDS             complement(3259..3909)
                     /locus_tag="PKI68_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53671.1"
                     /translation="MTPPVKAPSVPAANGTSYKVDKVTVSMIPYALPGDLKGKSFSDR
                     FARLAESRYPGLFKDTPEAVPVNVRIEISQEVHDGAALLTFMGTVGIFGGILPSLPWT
                     TEWQVKVGIEDRRGVRHAAEVQALHRGWWSLFSPCGLITIPGDSDIPAVSTVMTGGPG
                     SVPPELCDYVVRCMVDLTAKELLRENPVQMPEQPFLLMPGQPSAPLQMPAETVAPF"
     gene            complement(4017..>4634)
                     /locus_tag="PKI68_08000"
     CDS             complement(4017..>4634)
                     /locus_tag="PKI68_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53672.1"
                     /translation="NPNPQLSENYKKYQRQILPLLRKECAARYPALFTEDSSAAIPLG
                     VAAETKPTQHMLKTMGWMYGTLLISGVILPCPGDTDEDIAMKAGLWTGRDDLQSAALQ
                     KNFRREIHSWVSLLTPAALITIPGESDFPKVSGTLFNMQKMEEVYLQQLASQMATALA
                     ETVAAKDPAFWTAQPRRMESPSFSPVSPSAVPATLTLPTETIAPF"
BASE COUNT          808 a         1442 c         1385 g          999 t
ORIGIN      
        1 cagccgctga tattcccgca gcacgtcgcc ggtgaagtcc tggcagttgt gcgctccggc
       61 gttgtagctc tgcccttccg gatcggagag atacttttct tcccagcggg cttttacgtt
      121 tttaaccgct tccgtcatga cggcgccgtt tttgccgctc gccacgacgc ctttatactg
      181 cgcgcctgtt tccgatttga tgttcggcaa cgtcttcccg tcctggccgg cggcggttcc
      241 gttgaagtac ccgacgcttt cgccgttcgg cagcagaatc tgccagtgca tgccgtacgg
      301 attgtcgtcc cgcccggaca gtttattcac aacgtagggc gccgccttcc cgtccagatc
      361 ccgcgccgcg atcacccagc cgttttccgc cgtgatcttt gcgacctctt ccggctggta
      421 cggtttcagc gtcaccgttg catccttcgt tgcaatggct cgcgcgagtg tggagccgag
      481 gatcagatcc ttctccgttc catcccctgc cttcggcgac tctttcgccg ccgcgctctc
      541 tttctggaag tcgtattccg agggctgctt cggctccggt ttccgcagca gagcgggcgt
      601 gccgggggaa tcgccgagat gccgcagatc gaccccgttc ggatcgccgc cgctctttga
      661 cgggctgtcc caggcggcct cgtcggcaaa tacattccgg agctgctgcc gggcttcgcc
      721 gcttccgggg ctgtttaaaa gcgcgttctt gtcgttctcg aacgcctgcg cctgagtttg
      781 ctgttttttc agcgactgct cattgacctg tttcatctct ttgccccagg cctccgcgcg
      841 cgccgccaga ccgttggatt cgaagtggcg cgtccgaagt ccgccggccg tgaccgtgcc
      901 gtcataataa agccggtcat cctgcggctc gtccggactc cggcccggct gcggcatccc
      961 cggcgtaagc tgcggccgcg gttttgcttt ttgcacaaac gaatagccgc aggggcaggc
     1021 ggacggcatt tttccgttgg gacagtcgat ataccgtttg caggccggac accaggtgcc
     1081 ggcgagctga ggttttccaa cggccggaac gttctgcgcc cggccctccg gcggggcggc
     1141 aaccacaccg gcggagacga tcagcagcag cgcggcgccg cccgcgaaaa accgattccg
     1201 aatcttcaga tgcttcatag ttcctccgtt gccggggttt agaaggccgc aacagccgcc
     1261 agttcgcgca gtttctgttc ctgctgataa tcgaattcgt aaagccggtt caggatctcg
     1321 ccgacgttgg ccgcatcggg cttggccgcc agatacaatt tcaggtcgcc gacggcggcg
     1381 gcggtctggt tctgcagctt cttcacggcc gaggtgttga aatacagatc gccgacccac
     1441 ggcgagtaaa acaccgccca ctggaactcg gagagggcgc ggctcagatc gagcggattt
     1501 ttcgccgtct tcagagcaac gcttccgcgc agataatggt cctgagcgct gttgttcatc
     1561 gccggggccg gctccagtcc ggcggcggtc ttgagtaacg ttttgcgcac cgcttctttt
     1621 tcttttccgg ccgccgtgag gcgatagacg tcggccagtt ttgccagagc ctgatttctg
     1681 tcgccggccg ccagcgccgc gtcggcttcc gcgaggatcc gcttccgttc ggcggccgcc
     1741 tgttgttcga ggcgggtcag cgccagccgg gcccgggtgt aggccggcga gacctcgcag
     1801 caccggctga gaatttccat ggcttcggga atgcgcccgg ccttctcgaa agccagggcc
     1861 agcacgcacc actggttggc gccgccgcct ggcgtcagcg gaacaccggc gagctgtgtt
     1921 tcgagcgctt tgacggcttc atcgccctct ccggcggccg cgcggcagac ctgaatgtag
     1981 tccggcgctt tggttttcgc ggcgccaaaa gccgccagcg ctttgccgta ggcttcctgc
     2041 gcggcggcgg attctccggc ctgatcgtgg cactgggcga gcatgtacca ggcttttgaa
     2101 ttggagccgt cctgagcgac ggcttcgcgc agcagagccg tcgcttccgc cgtctgccgc
     2161 agattgaatt gcgcttcggc cagcagcagc gagatggcgt tggtcatctg gaagcgggcg
     2221 cgctgcgctt cgataaagcg gcggctttgg tccggcgagc cggcggcgac ctgccgggcc
     2281 ttcgtcagaa aactcaacgc ctcttcatag cgccagtgcg acaggcagac ggagccgatc
     2341 tccagcagct tgcagcaatc ggcggcgtac cgctcctgcc cggcgagaaa agcggcgaag
     2401 gctttatcgg actccgcatg catgccgagc gcgcggtagg cgtcggagag gcgcagcagc
     2461 gaggccggtt cgaccggcgc aatcgcatgg tatttggcga aggtggtgat gcactcgtcg
     2521 tatttgcaga gaaagtacag cgcttccgcc gacgtcttga gaacctccgt gcgcgacgga
     2581 tcgagctgca gcgcattgtc aaacgagagg atcgcctgct ggtaatcctt cttgccgaaa
     2641 taggcgaagc cgaggctgat atacaggccc gacacttcgc tcttcgcttt gagcgacgat
     2701 tccattttca tttccggaga cagcggcggc acgctgttcg gcaccgagtt gatctggtta
     2761 ttgacggttt gcagggtgct ggccatcatc tgggcctgca tattgccgtt catcccggcg
     2821 acaccggaga ccagcccgaa gagttcgttc atctcctgct tcgactgcaa atccttggcc
     2881 cgctgaatat cggcctcccg tttcgcgttc atcgcggccc gttgcgccgc ggcttcatcg
     2941 gcctgcttct tctcggcttc aataatcgtc gtgatcgaat gcagcgacag agcctgctgg
     3001 aacgccgcca ccgcctggtc gtactgcgcc tgccggtaat agaccgcgcc gaggtcgttg
     3061 aagacgcgcc agttctgcgg atcgcgggcg gcggcggcct tcgccgcggc aatctccgcg
     3121 gcacgctgcg ccgccgcggc gccggagttt tcctgcggcg tttgcgccca gcacacggcg
     3181 gatgaaaccg ccaacagaca ggccgtgaca ctccccgcag tcagcaaggt tagctttttc
     3241 attatttctc cttttcaatc agaacggcgc aacggtttcg gccggcatct gtaacggcgc
     3301 cgacggctgt cccggcatta ataaaaacgg ctgttccggc atttgtacag gattctcgcg
     3361 gagcagttct tttgccgtta aatcaaccat gcaccggact acatagtcac agagctccgg
     3421 aggaaccgat ccggggccgc cggtcattac cgtggaaacg gccggtatat cagaatcgcc
     3481 ggggatcgtg atcagaccgc agggcgaaaa caaactccac catccgcgat gcagcgcttg
     3541 aacctccgca gcgtgcctga cgccgcggcg atcttctata ccgactttga cctgccattc
     3601 tgtcgtccac ggtaaactgg gaagaatccc gccaaagatg ccgaccgttc ccatgaaggt
     3661 cagtaatgcc gcgccgtcat ggacttcctg gctgatttca atccgtacat taaccggaac
     3721 ggcttcgggt gtgtctttga acaggccggg atagcgcgac tccgccagac gggcaaaccg
     3781 gtcggaaaac gatttgcctt tcaagtcgcc gggaagtgca tagggaatca tcgagacggt
     3841 gactttatcc actttatatg aggtgccgtt cgcggccgga acagacggcg ctttcaccgg
     3901 cggtgtcata atgatcctat tggacgcaca tccgccgacg gataaaataa ctgtcatgca
     3961 tagaaataat ataaacggtt tcatcgtcgg catgatcagc tctccgcatt atgaatttaa
     4021 aatggcgcga tcgtttccgt cggcaaagtc agtgttgcag gcacagctga agggctgaca
     4081 ggcgaaaaag acggagattc catccgccgc ggctgcgccg tccagaacgc gggatctttc
     4141 gccgccacgg tttccgccag cgccgtcgcc atctgcgacg ccagctgctg gagatacacc
     4201 tcttccatct tctgcatatt aaaaagggtt ccggaaactt tggggaagtc ggattctccg
     4261 ggaatggtaa tcagcgcggc aggcgtcagc aggctgaccc agctgtgaat ttcccgccgg
     4321 aaatttttct gaagcgcggc ggactgcaga tcgtcccgtc cggtccagag tcccgccttc
     4381 atcgcaatgt cctcatccgt atctcccggg cagggaagaa tgacgcccga gatcaaaagc
     4441 gttccgtaca tccagcccat ggtcttcaac atatgctggg tgggcttggt ttccgcggcg
     4501 acgccgagcg gaatggcggc ggacgaatcc tccgtgaata gcgccggata gcgcgccgcg
     4561 cactccttgc gcagaagcgg cagaatctgc cgctgatatt ttttatagtt ctcggagagc
     4621 tgcggattcg gatt
//
LOCUS       DAOPZO010000136         4603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_24014_length_4603_cov_10.603562, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000136 DAOPZO010000000
VERSION     DAOPZO010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24014_length_4603_cov_10.603562"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PKI68_08005"
     CDS             <1..1020
                     /locus_tag="PKI68_08005"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNX53673.1"
                     /translation="QGELIIHRAGLVSGRAPEPGEIVLVDVDAERRADIQRHHTATHL
                     FHWALHNVVSADSRQRGSLVAPERLRFDFNSPEKLSAEQIAEIEKQVNERIQADEPVY
                     WTEVPFTEVQKHPEIQQFFGEKYGETVRVLQVGGASGSFNGFSMELCGGCHVRSTGDI
                     GLFKITSESSIAAGIRRIEAVCGKHAEQFIEEQKAAAAAEAERAKEVERQKQLAKQRQ
                     AEMQAKAPAIAEELLAKVSNGLLIENMGEADAELLSAVMDALQPKLTSAVAILGGVSD
                     GKVALVCAVTPDLVKAGKNAGKIVGAIAKQCGGGGGGKPDRAQAGGKLPEKLAEALAD
                     FANAR"
     gene            1036..1947
                     /locus_tag="PKI68_08010"
     CDS             1036..1947
                     /locus_tag="PKI68_08010"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNX53674.1"
                     /translation="MKNPMDRQLEKKLSDRAVAIGREGNAGAFDELLDLLRSSSANVR
                     RLSASALGKLAWMGVDQTKAVAALGPVARLDPHTQTRQYAIKALKAYGCASQAWLGDL
                     RDMAANAAEKEYIRRDAASAVEFIEEALRIAEASAVHRCQRCHAETTAEEFARSNQAF
                     QRIFCDRCFDEIFLERRNFETQVELNKTITAGDGTVVQSDGERRIADWLTAHGLTYRY
                     DAKYRIIGEFQIRPDFYLPELDVYVEYWGMDTPQYKMSMYKKQMLYQQEGKRLISVYP
                     QDLPRLNAVLSAKFRLFGYVVGNDASG"
     gene            1996..2208
                     /locus_tag="PKI68_08015"
     CDS             1996..2208
                     /locus_tag="PKI68_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53675.1"
                     /translation="MSEDKKIWFPIRKMGWGFPCCWQGRAVFLIYAALLATIYWLAPQ
                     SKIKAYGIGLTAILTVFLLFKGGRRR"
     gene            2256..>2570
                     /locus_tag="PKI68_08020"
     CDS             2256..>2570
                     /locus_tag="PKI68_08020"
                     /inference="COORDINATES: protein motif:HMM:NF036804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HNX53676.1"
                     /translation="MKPIDCPCGSGKMLSSCCGQWLSGKQSAPTAEALMRSRYTAYVL
                     KNVDYLVKTTLPAAREPNLADDIRSWMNQVTWQRLHVLSTKAGGRSDNEGTVEFIAEF
                     IGP"
     assembly_gap    2571..2670
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2806..3594)
                     /locus_tag="PKI68_08025"
     CDS             complement(2806..3594)
                     /locus_tag="PKI68_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="platelet-activating factor acetylhydrolase IB
                     subunit"
                     /protein_id="HNX53677.1"
                     /translation="MKKKLLLPILCISAMLFNGCLGLENAAPCDVVIGSHKAVTPAPR
                     ADVAWWMPRHQAVLDRISQGNVDLLMIGDSITHGWEKSGKATWNKYYAPRNAVNMGFS
                     GDRTEHVLWRLQNGEIKNIKPKLAVLMIGTNNSNGDQYTAEQIADGIKAIVCTLRTNL
                     PETKVLILAIFPRGDAGQSQDKEHDAAYNEQWAKNDQASRLASKLADGKMVFYLDVNK
                     AFLNEQGVLTREIMPDLLHPKEKGYQLWAAAMEPTIAKLMGETK"
     gene            complement(3720..>4603)
                     /locus_tag="PKI68_08030"
     CDS             complement(3720..>4603)
                     /locus_tag="PKI68_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNX53678.1"
                     /translation="YDGLMETAADIETTLDFVEDELPDDVFPNLGRKLDESFQNLETL
                     LATWDEGRLLREGARVVILGRPNAGKSTLFNALLGHDRAIVTALPGTTRDVLEETLVL
                     DGVPLRILDTAGLRETECLIEQEGIRRARVHSAAADIAVYLIDFSQPFSSEDSGHLAR
                     LDPRRTVVILNKADKKISGFRFQVPGFQPLETSLVNAQGIDGIKAAIADRLEAGVDFH
                     APAHAVISERHRNLLETARTELEQARERIAEGGETAAAPAAEHLRAALEALGQVTGRV
                     YHTELLDNVFSRFCIGK"
BASE COUNT          980 a         1280 c         1305 g          938 t
ORIGIN      
        1 cagggcgaac tgatcatcca ccgcgccggg ctggtttccg gccgcgcgcc ggagccgggc
       61 gaaatcgttc tggtggatgt cgatgcggag cgccgcgccg atattcagcg ccaccacacc
      121 gccacgcacc ttttccactg ggcgctgcat aacgtcgtca gcgccgactc gcgccagcgc
      181 ggctcgctcg tcgcgccgga acggctgcgc ttcgacttca actcgccgga aaaactgtcc
      241 gctgaacaga tcgccgaaat cgaaaagcag gtcaacgaac ggatccaggc cgacgagccg
      301 gtttactgga ccgaagttcc cttcaccgaa gtgcagaagc atccggaaat ccagcagttc
      361 ttcggcgaga agtatggaga aacggttcgt gtgcttcaag tgggtggagc ctcaggctcc
      421 ttcaatggat tctccatgga actgtgcggc ggctgtcacg tccggagcac cggcgacatc
      481 ggcctcttca aaatcaccag tgaatcatcc atcgccgccg gtatccgccg catcgaagcc
      541 gtctgcggaa aacacgccga acagtttatc gaagaacaga aagccgccgc ggccgctgaa
      601 gccgaacgcg ccaaagaagt cgaacggcag aaacagctcg ccaaacagcg ccaggccgaa
      661 atgcaggcta aagctccggc gattgcagaa gaactgctcg ccaaagtttc aaacggtttg
      721 ctcatcgaaa acatgggcga agccgacgcc gaactgctca gcgccgtcat ggatgcgctt
      781 cagccgaaac tcaccagcgc cgtcgccatc ctcggcggtg tttccgacgg aaaagtcgcc
      841 ttggtttgcg ccgtcacgcc cgatctcgtc aaagccggca agaacgccgg aaagatcgtc
      901 ggcgccatcg ccaaacagtg cggcggcggc ggcggcggca aacccgaccg cgctcaggcc
      961 ggcggcaaac tgccggaaaa actggcggaa gctctggctg actttgctaa cgcgcgttga
     1021 agcggggaga acaccatgaa aaatccgatg gaccgacagc tcgaaaagaa actttcggat
     1081 cgggctgtgg ccatcggccg cgaagggaat gcgggcgctt ttgatgaact gctggatctt
     1141 ctcaggtcgt cttccgccaa cgtccgcaga ctgagcgcat cggctctcgg caaactggcc
     1201 tggatgggag tcgatcagac aaaggctgtc gccgcactcg ggccggtcgc ccggctcgat
     1261 ccgcataccc agacgcgaca gtatgcgatc aaagcactca aggcgtacgg atgcgcctct
     1321 caagcgtggc tcggagacct gcgcgacatg gctgccaatg ccgccgaaaa agaatatatc
     1381 cgccgcgacg ccgcctcggc ggtcgaattc attgaagagg cgctgcgcat cgccgaagcc
     1441 tccgccgttc accgctgtca aaggtgtcat gccgaaacaa ccgcagagga gttcgcccgc
     1501 tccaatcagg cgtttcagcg cattttctgc gaccgatgct tcgatgaaat ttttctggaa
     1561 cggcgcaatt ttgagacgca ggttgagctg aataaaacca tcaccgccgg agacggcacc
     1621 gtggttcagt ccgacggcga gcgccggatt gccgactggc tgaccgcgca cggcctaacc
     1681 tatcgctacg acgccaaata ccgcatcatc ggcgaatttc agattcgtcc cgatttttat
     1741 ctgccggaac tggatgtata tgtcgaatac tggggcatgg acacgccgca gtacaaaatg
     1801 agcatgtaca aaaaacagat gctctatcag caggaaggca aacgcctgat ttcagtttat
     1861 ccgcaggatc ttccacgact caacgccgta ctatcagcga aattccgttt gttcggctac
     1921 gttgtcggca acgatgcgtc cggctaagcg gacattgacg cgcgaagcaa agccagcgac
     1981 aaacagggaa aaaccatgag tgaagataag aaaatttggt ttccgatcag gaaaatgggc
     2041 tggggattcc cttgctgctg gcaaggccgg gcggtttttc tcatctatgc tgcgcttctc
     2101 gcaaccattt actggctggc gccacaaagt aaaatcaaag cctatgggat cggattaact
     2161 gcgatcctca ccgtctttct tttatttaaa ggcggtcgtc gccgctaata agaaacaaca
     2221 aagccctctg cctcgtcatc tattctggag atactatgaa acccatcgac tgcccgtgcg
     2281 gaagcggaaa aatgttgtcg agctgttgcg ggcaatggct gtccgggaaa caaagtgcgc
     2341 cgacagcgga ggcgctgatg cggtcgcgct acacggccta tgttttgaag aatgtcgatt
     2401 atctggtgaa gactaccctg cctgcggcgc gcgaacccaa tttggcggac gatatccgca
     2461 gctggatgaa tcaggtgacg tggcagagac tgcatgtgct cagcaccaaa gccggcggac
     2521 gttccgataa cgaaggaacc gttgaattca tcgccgagtt tatcggcccg nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agccgcttta tctttccggt cattaaactt
     2701 ttccgatcac tgtggagttt cctcgcagag gttcagagtg cgcagagcct ttgcgcgctc
     2761 cgcgccttgg cgatgaattt ctccagcgtc tggcagaaac ggcggttact tggtttcgcc
     2821 catgagtttg gcaatcgtcg gctccatggc cgcagcccag agctgatagc ctttttcttt
     2881 ggggtgcagc aggtcaggca tgatttcgcg ggtcagtacg ccctgttcgt tcaggaaggc
     2941 tttattgaca tccagataga acaccatttt tccgtcggcc agtttcgaag cgagccggct
     3001 ggcctggtcg ttcttggccc actgctcgtt ataggccgca tcatgttcct tgtcctggct
     3061 ctgcccggcg tcgccgcggg ggaagatggc caggatcaga actttggtct ccggcagatt
     3121 cgtgcgcagg gtgcagacaa tcgccttgat gccgtcagcg atctgttcgg ctgtgtactg
     3181 gtcaccgttg gagttgttgg tgccgatcat cagcaccgcc agcttgggtt tgatattttt
     3241 aatctcgccg ttctgcagcc gccagagaac atgttcggtg cggtcgccgc taaagcccat
     3301 attgacggcg ttgcgcggag catagtattt gttccaggtc gctttgccgc ttttttccca
     3361 tccgtgcgtg atggagtcgc cgatcatcag caggtcaacg ttcccctgtg aaatccggtc
     3421 gagaaccgcc tgatggcgcg gcatccacca tgcgacatcc gcgcgcggcg ccggcgttac
     3481 agccttatgc gaaccgatga caacatcaca gggcgcggcg ttctcgagcc ccagacatcc
     3541 gttgaacagc atcgcggaaa tacacagtat cggcagcagc agtttcttct tcataggatc
     3601 ctttcagtat tttcagtctg gaggggatat ctgttataaa atgcaaatcc tccggaacga
     3661 ttacaagtcc ggaccgtttt ctttgtcgtg ccgccggcgg cggcgaagtt ctatatggat
     3721 catttcccga tacagaagcg ggagaataca ttgtcgagca gttcagtatg gtaaacgcgg
     3781 ccggtgacct gaccaagagc ttcgagcgca gcgcgcagat gttcggctgc cggcgcggca
     3841 gcggtttcgc cgccctccgc aatccgttcg cgcgcctgtt ccaattcagt ccgcgcggtt
     3901 tccagcaggt tgcgatggcg ttcggaaatc acggcgtgcg ccggagcgtg aaaatcaacg
     3961 ccggcttcaa gccggtcggc aatggccgct ttaatgccgt cgattccctg cgcattgacc
     4021 agcgaagttt ccaggggctg gaaacctgga acctgaaacc tgaagcctga aattttttta
     4081 tccgctttat ttaaaatgac gaccgtgcgc ctgggatcaa gtctggcgag atgcccgctg
     4141 tcttcagaag aaaacggctg agagaaatca atgaggtaaa cggcgatgtc ggcggcggcc
     4201 gagtgaacgc gggcgcggcg gataccttcc tgttcgatga ggcattcggt ttcgcgcagt
     4261 ccggcggtat cgagaatgcg caacggaacg ccgtcgagaa caagggtttc ttcgagaaca
     4321 tcgcgggtgg tcccgggcag cgcagtgacg atggcgcgat cgtggccgag cagcgcgttg
     4381 aagagagtcg atttgccggc gtttggacgg ccaaggatga caacgcgcgc tccctcgcgc
     4441 agcagacgtc cttcgtccca ggtggcgagc agggtttcca gattttgaaa actttcgtcc
     4501 agtttcctgc cgaggtttgg aaatacgtcg tccggcagtt cgtcttcaac aaagtcgagc
     4561 gtggtttcaa tgtcggcggc ggtttccatg agtccgtcgt aaa
//
LOCUS       DAOPZO010000137         4601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_24035_length_4601_cov_7.818522, whole genome shotgun sequence.
ACCESSION   DAOPZO010000137 DAOPZO010000000
VERSION     DAOPZO010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24035_length_4601_cov_7.818522"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /gene="rlmN"
                     /locus_tag="PKI68_08035"
     CDS             complement(<1..980)
                     /gene="rlmN"
                     /locus_tag="PKI68_08035"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNX53679.1"
                     /translation="MNSVYDIQALESVCRDPHRRKLFCNAFFKKALPLEVCLEKAPEL
                     NGRLNFETLEIVERQDSQRDGATKMVLRAADGHKIEAVILRIASGRTSLCISSQVGCA
                     ADCRFCATGKLGFIRNLTAAEMLDQVLLAKRLLREEGRTLRNVVVMGMGEPLRNEENL
                     FQCLETLRDKRFFNLSDNHLLVSTVGIPDAMLRFAERFPKIRLALSLHSARQEVRERI
                     MPVAKSQTLEKLKAVFPALENTAGFMVEYLMLKGINDEPEDIAALIEFLRGTRAHINL
                     IQFNAHPGAQFEPVSKEAREAFGDELKRAGFKVTLRYSLGDDIAAACGQLA"
     gene            complement(1109..3226)
                     /locus_tag="PKI68_08040"
     CDS             complement(1109..3226)
                     /locus_tag="PKI68_08040"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF007209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HNX53680.1"
                     /translation="MSEEQEFLFGDEPGEEKKTAKKNAAKKKPVEADDAPDESADSGK
                     RPRTHLALDSEHGPLRKLVDYNFLQYASYVICDRAIPNVEDGLKPVQRRIMHALSEKD
                     DGRFIKVANVVGHTMQYHPHGDASINDALVNLANLGGYLIEGQGNFGNIYTGDRAAAP
                     RYIECRLTPLAREELFNKELTRYIPSYDGRNKEPVVLPCKLPLLLMLGADGIAVGLST
                     KILPYNFIELLEEQIEILHEKVRTPHKCTALPDFQTGGLMDVSEYDNGNGKVKVRAKI
                     EERGGNRLIITDLPYGQTTESLTTSIEEAVRKKKVAVRAINDFTAENVEIELVLSVGA
                     DPEQVKQALYVFTACESAISSRIVVIHNNRPVEMTVDEILHLNTKQLLTILEAELNLK
                     KDKLLDAFHTKTLVQIFVENRIYKKIEQCKTYETVHQAVFTGLEPFLHLLRRPVVTDD
                     VEMLLAIQIKRISLFDMNKNKEEIEKILTELAEVEKNLKSLTVYATRYLKDLIKKYKD
                     RYPRLTQVKTFKEIEVRELTATELHIQHDMESHFVGSGVKGESLLKCSSLDKLIFVWD
                     DGRYQMMAPPEKFFVDGNLLYSAIFDRDREMTCVYVSGRETYIKRFTFGGAIMNRDYQ
                     LAPEGSKILFFQEGCPDELYLRYRPAKGQRIHQQFFKLKDLAVKGSKAKGNRLTTKSV
                     QYIDTRPGRWWRDDEEAPQGVLL"
     gene            complement(3223..>4601)
                     /locus_tag="PKI68_08045"
     CDS             complement(3223..>4601)
                     /locus_tag="PKI68_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNX53681.1"
                     /translation="EAEDKGTCKAEENGTFISFSPDPEIFKVYKFREDHIERRLRFYS
                     YLNAGLTLLFNGKKIISDGGLLDLIADESEFEKIYEPFHYRDKMLELAFTHTNRFSED
                     YYSFVNGQFTSDGGTHLSAFREGLLKAVNEYSDGKFDGDDVREGILGAIAIRLKEPIF
                     ESQTKNKLGNTEIRADLVADIKKAIVEMLHRNRPEADRLIEKIKDSQRLRKELQDVKK
                     LARERSKAVSIRVPQLKDCKQHFSKEKGTHADTMIFITEGQSAAGSLVSCRDVNKQAI
                     YTLKGKPLNVWDLKRDALYKNVELYNLMRSLNIEDSAADLRYGHVILATDADVDGLHI
                     RNLMITFFLRFFEPLVRDGHLKILETPLFRARNKKETIYCYSEKERDTALEKLGRGAE
                     VTRFKGLGEISPGEFKAFIGEEMRLTPVVVDEDHSIPEVLTFYMGKNTQERRTYIMDN
                     LVVDEEIL"
BASE COUNT          922 a         1182 c         1319 g         1178 t
ORIGIN      
        1 gccagttgac cgcaggcggc ggcgatgtcg tcgccgaggg aatagcgcag ggtcactttg
       61 aatccggcgc gtttaagttc atcgccgaac gcttcgcggg cttctttgga aaccggttcg
      121 aactgcgcgc cgggatgcgc attgaactgg atcaggttga tgtgcgcacg ggtgccgcgc
      181 aggaattcaa tcagagcggc gatatcttcc ggttcgtcgt tgatgccttt gagcatcagg
      241 tactcgacca taaatccggc ggtattttcg agcgccggaa aaaccgcttt cagtttttcc
      301 agcgtctgcg acttggcgac cggcataatc cgctcgcgga cttcctgtcg cgcactatgc
      361 agacttagcg ccagccggat tttcggaaag cgttccgcga agcgaagcat tgcatccgga
      421 atgccgacgg tggaaaccag caggtgattg tcggagagat taaagaagcg tttatcgcgc
      481 agggtttcca ggcattggaa cagattctct tcattgcgca gcggttcgcc cattcccatc
      541 acaacaacgt tgcgcagcgt ccggccttct tcgcgcagaa gccgcttggc gagcagtacc
      601 tgatcgagca tttccgccgc cgtcaggttg cggataaacc cgagtttgcc ggtcgcacag
      661 aaccggcagt cggccgcgca gccgacctgc gaggaaatgc agagactcgt ccggcccgaa
      721 gcaatgcgta aaatcacggc ctcgatcttg tgtccgtccg ctgcccgcaa gaccatttta
      781 gttgcaccgt cgcgctgcga gtcctgtcgc tcgacaatct caagcgtttc aaagttcagc
      841 cttccattca gttccggagc tttctccaga cacacttcga gcggcagcgc ttttttaaaa
      901 aaggcgttgc agaaaagttt ccgccggtgc ggatcccggc agaccgattc caaagcctga
      961 atgtcgtata cggaattcat ttttaaccgc agattagaaa cacccacgaa tcacatcaac
     1021 ttcagttttt tgtacaaggc cggcaagatg ccggcgatac gtacagcagg cccccagcct
     1081 gcaaaaaaat tcaatgccgc acagggtttt acagcagaac gccctgcggg gcttcttcgt
     1141 cgtcgcgcca ccagcggccc ggcctggtgt cgatatactg caccgatttg gtggtgagcc
     1201 ggttgccttt ggcttttgag cccttgaccg ccaggtcctt gagtttgaag aactgctgat
     1261 gaatgcgctg gcctttggcc gggcggtagc gcaggtaaag ctcgtcgggg catccttcct
     1321 gaaagaagag tattttcgaa ccttcgggcg caagctggta gtcgcggttc atgatggcac
     1381 cgccgaaggt gaagcgtttg atgtaggttt cgcggccgct cacatagacg caggtcattt
     1441 cgcggtcgcg gtcaaagatg gcgctgtaga gcaggttgcc gtcgacgaag aatttttccg
     1501 gcggggccat catctggtaa cgtccgtcgt cccagacaaa gatgagctta tcgagtgaag
     1561 aacatttcag gagagattcg cccttaacgc cggaaccgac aaaatggctt tccatatcat
     1621 gctggatatg gagttcggta gcggtgagct ctcgtacttc gatctctttg aaggttttaa
     1681 cctgggtgag gcgcggatag cggtctttat attttttgat gagatctttg aggtagcggg
     1741 ttgcgtacac cgtgaggctc ttgaggtttt tctccacttc ggccagctcg gtaaggattt
     1801 tttcgatctc ctctttgttt ttgttcatgt cgaagagcga gatgcgtttg atctgaatgg
     1861 caaggagcat ttcgacgtcg tccgtcacca cggggcggcg cagcaggtgc aggaagggct
     1921 ccagccctgt gaagaccgcc tgatggactg tctcgtaggt tttgcactgc tcgatttttt
     1981 tgtagatgcg gttttcgaca aagatctgca cgagcgtttt ggtatgaaag gcgtcgagca
     2041 gtttatcttt tttaagattc agctctgctt cgagaatggt gagcagctgt ttcgtattca
     2101 ggtgcaggat ttcatcgacc gtcatttcga ccgggcggtt gttatgaatc acgacaatgc
     2161 ggctggaaat ggctgattca caggcggtga aaacatacag cgcctgcttg acctgctccg
     2221 ggtcggctcc gactgaaaga accagttcga tttcgacgtt ttcggccgtg aagtcgttaa
     2281 tggcacgaac ggccactttt ttcttccgca cagcctcttc gatcgatgtg gtgagcgatt
     2341 cggtggtctg tccgtacggc agatcggtga tgatcagccg gttgccgccg cgttcttcga
     2401 ttttcgcgcg gactttgact ttaccgtttc cgttgtcgta ttcggaaaca tccatgagtc
     2461 cgccggtctg aaagtccggc agcgcggtgc atttatgcgg tgtgcgcacc ttctcgtgga
     2521 gaatttcaat ctgctcttca agcagttcga tgaagttgta gggcagaatt ttggtcgaga
     2581 gtccgacggc gatgccgtct gcgccgagca tgagcagcag cggcagcttg cagggaagaa
     2641 caaccggctc tttattccgt ccgtcatagc tggggatata gcgggtgagt tctttattaa
     2701 aaagttcctc gcgggccagc ggagtcagac ggcactcgat gtatcgcggc gcagcggcgc
     2761 ggtcgccggt gtagatgttg ccgaagtttc cctgcccttc aatgaggtat ccgccgagat
     2821 tcgccagatt cacaagggcg tcattgatgg aggcgtcgcc gtgcggatga tactgcatgg
     2881 tatgaccaac gacgttggca actttgatga agcgtccgtc gtctttttcc gacagcgcgt
     2941 gcatgatgcg gcgctgcacc ggtttgaggc cgtcttcaac attgggaatc gcgcggtcac
     3001 aaattacata ggaagcgtat tgcagaaagt tgtagtccac cagtttccgg agcgggccat
     3061 gttcggaatc aagagcgagg tgtgtccgcg ggcgctttcc ggaatcggcc gattcatccg
     3121 gcgcatcatc cgcctcaacg ggtttctttt tcgccgcatt ttttttggcg gttttttttt
     3181 cttctcccgg ctcgtcgccg aaaaggaatt cctgttcttc gctcatagaa tttcttcgtc
     3241 cactaccaga ttgtccataa tgtaagtgcg gcgttcctgc gtattcttgc ccatgtaaaa
     3301 ggtgagcact tcaggaatcg agtggtcttc atcgaccacc accggagtga gccgcatttc
     3361 ttcgccgata aaggctttga attcgccggg cgaaatttcg ccgagtcctt tgaaccgggt
     3421 gacctctgcg ccgcgtccca gcttttcaag cgcggtgtcg cgctcttttt cggaatagca
     3481 gtagatggtt tctttcttgt tgcgggcgcg gaacagcggc gtttcgagaa ttttcagatg
     3541 gccgtcacgc accagcggtt caaaaaagcg caggaagaaa gtgatcatca gattgcggat
     3601 gtgcagaccg tccacgtcgg cgtcggtggc aagaatgacg tgaccgtagc gcaaatcagc
     3661 cgcggagtct tcgatgttga gactgcgcat gagattgtag agttctacat ttttatacag
     3721 cgcgtcccgc ttgaggtccc agacattcag cggctttccc ttaagcgtat aaatcgcctg
     3781 tttgttcacg tcacggcagc tgaccaacga accggccgcc gactgacctt cggtgataaa
     3841 gatcatcgta tcggcatgag ttcccttctc tttgctgaag tgctgcttgc agtctttgag
     3901 ctgcggaacc cggatggaaa cggccttgga gcgttcgcgc gccagctttt ttacatcctg
     3961 aagctctttg cgcaggcgct ggctgtcttt gatcttttcg atcagcctgt cggcttcggg
     4021 ccggttgcgg tgaagcattt cgacaatggc ttttttaatg tcggcgacca gatccgcgcg
     4081 gatttcggta ttgccgagtt tgtttttggt ctgcgattca aagatgggtt ctttgaggcg
     4141 aatggcgatg gcgccgagaa tcccctcgcg cacatcgtcg ccgtcgaact tgccgtcgga
     4201 gtattcattg acggccttga gcaatccttc gcggaaggcg ctcagatggg tgccgccgtc
     4261 gctggtgaac tgcccgttga cgaacgagta ataatcctcg ctgaagcggt tggtgtgcgt
     4321 aaaagcgagt tcgagcattt tgtcgcgata gtggaacggc tcgtaaattt tctcgaattc
     4381 gctttcgtcg gcgatcagat cgagcagccc gccgtcggaa ataatttttt tgccgttgaa
     4441 cagaagcgtg aggccggcgt tgagatagga ataaaaccgc aggcggcgct caatatgatc
     4501 ctcgcggaat ttatacactt tgaaaatttc agggtccggc gagaagctga tgaaggtgcc
     4561 gttttcctcg gctttgcagg tgcctttgtc ttccgcctcc a
//
LOCUS       DAOPZO010000138         4538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_24537_length_4538_cov_8.976132, whole genome shotgun sequence.
ACCESSION   DAOPZO010000138 DAOPZO010000000
VERSION     DAOPZO010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24537_length_4538_cov_8.976132"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1031
                     /locus_tag="PKI68_08050"
     CDS             201..1031
                     /locus_tag="PKI68_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53682.1"
                     /translation="MKIRWHVMAGSLLIAGVMQAQATVMWDGGGAANTALSNPTNWAG
                     DTLPVNDPDKIGIIGGSYTAARTYITDFTGYHLILESNAVLTNRVGGSGTAVSTMYLT
                     NFTLTVRDNARFTVPDRTDSLNLNQAATVNVSNNAVLELGILRLSGETAGSVGNFYQY
                     GGTVSANKLDINRAGTGIYHLYGGTLTVTNATKSSNNLTGSNYIDFATDSKGVFQWTG
                     NQTNNMVGLITNGLIRFENRTLSPTNFLITYNSTNNLSSVQLSPELFKRVKLFIITGN
                     "
     gene            1144..2064
                     /locus_tag="PKI68_08055"
     CDS             1144..2064
                     /locus_tag="PKI68_08055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNX53683.1"
                     /translation="MEKVAEKQEARLDAWLAEAVPEHSRSRWQSLIKDGRVTVNGAPC
                     KANQKIRSGDRAEWTVPEAAPAQPQAEDISLNILYEDRNMIVINKPAGLVVHPAVGNE
                     TGTLVNALLHHCKDLTGIGGEKRPGIVHRLDKDTSGIMVVAKNEQAMTELARQFKERE
                     TAKEYLTIVWGVPYGKRGTVETTIGRNPLHRKKMAANVRNGREAVSHYEVLEALGEAA
                     LVQVKIETGRTHQIRVHMAHIKNPVIGDTVYGRGRASPIKAGRQMLHAAKLSLNHPVS
                     GKRMTFEAPLPADMETLLTQLRALHAPAKR"
     gene            2119..3273
                     /locus_tag="PKI68_08060"
     CDS             2119..3273
                     /locus_tag="PKI68_08060"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX53684.1"
                     /translation="MNSKTVIVGGGFAGRQACRALRRADTEVILFDPRSSTVMLPALP
                     DLAGGWLDEKLLHFPLAELLPASVRHIQKAVESIDLDAGTLVAGGETAAFDALLIASG
                     SVTDFRGFDQHREQIYHLDSLETARHIRDDFGSYLMQTERPHVVMAGGGYTGLELAMS
                     LYYRALAANNPCRVTVIDPAPDILPFLPAENQKYIKAFLARTGVTVLSGMRVTGFDGK
                     TVTAGETRFENVFFCWAGGSTFAVPELKGAVARLGDGRLNVRSDLSLPNYPTVFAAGD
                     AAAVEHNGRILRKAVNFAWYEGKHAGKNIARKLRGLPAKPFRPFDAGWVIPLHGDSVG
                     QLFSRLWIKGTDGLRLHYLMCGFRNYCFSNFAGFAKVAAKLFKKEAHYEG"
     gene            3263..3679
                     /locus_tag="PKI68_08065"
     CDS             3263..3679
                     /locus_tag="PKI68_08065"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNX53685.1"
                     /translation="MKVKQILFGTTPDANIGILLLRVFTGAALMTHGIAKMAGGMEKF
                     TGFVASLGVPAPHVMAFLAAFSESFGAAFLAVGLLTRPAAFMIICTMSVAIFGALGGQ
                     PFAKQELAWLYFFPALMFLLKGAGRWSIDFMVTGKK"
     gene            3687..>4538
                     /gene="menA"
                     /locus_tag="PKI68_08070"
     CDS             3687..>4538
                     /gene="menA"
                     /locus_tag="PKI68_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HNX53686.1"
                     /translation="MNRDFQNWARALRAYSFTATVIPVLCAFLSARAQGLPVAWTLLP
                     LMLFCALLLHAGVNLLNDYYDFVLGFDTAEARGSSGLLTEGIVRPEYMRRRGRFYIFA
                     GASAGLLLAAARGWPLLLMGIAGLAGAWFYSHRKGYKYKGLGEPFVFVLMGLLLFGGA
                     FYTATGTITLGAVWPALACGCLVTAILLVNNLRDIQMDGQAGFTTLPIRIGATRTKNL
                     YAALVVTALIATCAAGIFLPLLSLPLAWRRIHSVQTATEPSHDLADAPQQTAAFYLIF
                     GVLLAAGL"
BASE COUNT          966 a         1338 c         1277 g          957 t
ORIGIN      
        1 gggggtcttt cgctttccgg gacggcccgc tctccgccgc tatcgtccgg cagaaccccc
       61 tgccgttgtc cggcgtttaa actttcagcc tgtcaactga tacaaaaacc taaaaatatc
      121 tcgccattcc tgccccaaac cctctatttt aacgcctgtt tgagcgtact attcagcaag
      181 cagaaataaa ggaagtcctc atgaagatac ggtggcatgt aatggcagga tctctgttga
      241 tcgcgggcgt gatgcaggca caggcgacag taatgtggga cggcggcgga gccgcaaata
      301 ctgcgctgag caacccgacc aactgggccg gcgacacact gcccgttaac gatccagaca
      361 aaatcggcat tatcggcggc tcatatactg ccgcgcgcac ttacataacg gactttaccg
      421 gatatcattt gattcttgag agcaatgcgg ttctcacaaa cagagtgggc gggtccggga
      481 ccgctgtatc cacaatgtat ttgaccaact ttacactgac cgtacgcgat aacgcccgtt
      541 ttacggtgcc ggatcgcacc gacagcctga acctcaacca ggccgccacc gtgaacgtga
      601 gcaataacgc tgtgctggag cttgggattt tgcgtctgtc gggcgaaaca gcaggctccg
      661 tcggaaactt ctaccaatac ggcggtactg tatcagccaa taagctcgac attaaccgcg
      721 ccggaaccgg tatctatcat ctgtacgggg gcactctgac cgtcacgaat gcgacaaaga
      781 gctccaataa tttgaccggc agcaattaca ttgactttgc gaccgacagc aaaggggtct
      841 tccaatggac cggaaatcaa accaacaaca tggtcggcct gattacaaac ggcttgattc
      901 gtttcgaaaa cagaacactc tcgccgacga attttttgat tacatataat tcaacgaaca
      961 acctgagctc cgtccagctt tccccggagt tattcaaacg ggtcaaactg ttcatcataa
     1021 ccggaaacta ggccgtccct tttctttcaa attgccggtg cagcaaattt aaggccggat
     1081 gtgtttaccg aagagccgta gcttgtgttg ctaaagcgaa ggcggccggt taccatccgc
     1141 gccatggaaa aagttgcaga aaaacaggaa gcgcggctgg acgcatggct ggcggaagct
     1201 gtaccggaac attcgcggtc gcgctggcag tcgctgatta aagacggacg cgtcaccgtt
     1261 aacggcgcac cgtgcaaagc caaccagaaa atccgttccg gcgaccgcgc ggagtggaca
     1321 gttcccgaag cagcgcccgc tcaaccgcag gcggaagata tttcgctgaa cattctctac
     1381 gaagaccgca acatgattgt catcaacaaa cccgccgggc tggtggtgca tccggcggtc
     1441 ggcaacgaaa ccggtacgct ggtcaacgcc ctgctccacc actgcaaaga cctgaccggc
     1501 atcggcggcg aaaagcgccc cggcatcgtc caccggctgg acaaagatac cagcggcata
     1561 atggtggtcg ccaaaaacga acaggcgatg accgagctgg cgcggcagtt taaggaacgc
     1621 gaaaccgcca aggaatatct gaccatcgtc tggggcgttc cgtacggcaa gcgcggcacg
     1681 gtcgaaacga cgatcgggcg caatccgctg caccgcaaaa aaatggcggc aaacgtccgg
     1741 aacggccgcg aagcggtttc gcattacgaa gtgctggaag cactcggcga agcggcgctg
     1801 gtacaggtga agattgaaac cggccggacg catcagatcc gcgtccacat ggcgcacatc
     1861 aaaaaccccg tcattggcga tacagtctat ggccgcggac gggcttcgcc gatcaaggcc
     1921 ggccgccaga tgctgcacgc cgcaaaactt tcgctgaacc atccggtctc cggcaaacgc
     1981 atgacctttg aagcgccgtt gcccgctgat atggaaacac tgctcacgca gttgcgggcg
     2041 ctgcacgctc cggcaaagcg gtaatttctg atttgagttt ttgcattgaa ccggctccgg
     2101 gcttattctg agcgcgtgat gaacagcaaa acggtcattg ttggcggcgg cttcgccgga
     2161 cggcaggcct gccgcgcttt acgccgtgcg gacacggaag ttatcctgtt cgatccgcgc
     2221 agttcaacgg ttatgctgcc cgccctgccc gatctcgccg gcggctggct ggatgaaaaa
     2281 ctgctgcact ttcctctggc agaactgctt ccggcttctg tccggcatat tcagaaagcg
     2341 gttgaatcca ttgatctcga cgccggaacg ctggttgcgg gcggcgaaac ggctgccttt
     2401 gatgcactgc tgatcgcatc cggatcggtg accgatttcc ggggcttcga ccagcaccgc
     2461 gaacagattt accatctcga ctcgctcgaa acggcgcggc atatccgtga cgatttcgga
     2521 tcgtatctga tgcagaccga gcggccgcat gtggtgatgg cgggcggcgg ctacaccggc
     2581 ctcgaactgg cgatgagtct ctattaccgc gctctggccg caaataaccc ctgccgcgtg
     2641 acagtaatcg atccggctcc ggacattctg ccgttcctgc cggccgaaaa tcagaagtat
     2701 atcaaagcgt ttctcgcccg gaccggcgta accgttcttt ccggcatgcg tgtcaccggt
     2761 tttgacggaa agactgtaac cgccggggaa acacgctttg aaaatgtttt cttctgctgg
     2821 gcgggcggtt cgaccttcgc cgttccggag ctgaaaggag ctgttgcgcg gctgggcgac
     2881 ggacggctga atgtgcgctc cgatctttcg cttccgaact atccaacggt tttcgccgcc
     2941 ggcgacgccg cagcggtcga acacaacggc cggattctgc gcaaagcggt gaatttcgcg
     3001 tggtacgaag gaaagcatgc cgggaaaaac attgcgcgaa aactgcgcgg acttcccgcc
     3061 aaacccttcc gtccgtttga cgcaggctgg gtgattccgc tccatggcga cagcgtcgga
     3121 cagcttttca gccgcctgtg gatcaaagga acggacggac tgcggctgca ttatttgatg
     3181 tgcggtttcc gcaattactg tttttcaaat tttgccggat ttgccaaagt cgccgcgaaa
     3241 ctgttcaaga aagaggctca ttatgaaggt taagcagatt ctcttcggaa caactccaga
     3301 tgccaatatc ggcatccttc tcctgcgcgt cttcaccggc gcggcgttaa tgacacacgg
     3361 catcgctaaa atggccggcg gaatggaaaa gtttacggga ttcgtcgcca gtctcggcgt
     3421 acccgctccg cacgtcatgg cgtttctggc cgctttctcg gaaagcttcg gcgcggcatt
     3481 tctggcggtc ggcctgctga cccgtccggc ggcctttatg atcatatgta ccatgagcgt
     3541 cgccattttc ggcgcgctcg gcggacagcc gttcgccaag caggaactgg cctggctcta
     3601 tttcttcccc gcactgatgt tcctgctgaa aggcgccgga cgctggtcga tcgacttcat
     3661 ggttacggga aagaaataat ccggacgtga accgcgactt ccagaactgg gcgcgcgcac
     3721 tgcgcgccta ttcttttacc gccaccgtca ttccggtgct ttgcgccttt ctgtccgccc
     3781 gcgcgcaagg gcttccggtt gcgtggacgc tgctgccgct gatgctgttc tgcgcgctgc
     3841 tgctgcacgc cggagtcaat ttgctgaatg attattacga cttcgttctc ggcttcgaca
     3901 ccgccgaggc gcgcggctca agcggactgc tgacagaagg aatcgtccgt ccggaataca
     3961 tgcgccgccg gggccggttt tatattttcg ccggcgcatc ggcaggcctg ctgctggcag
     4021 cggcacgcgg ctggccgctg ctgctcatgg ggatcgccgg tctggccggc gcgtggtttt
     4081 attcacaccg gaaagggtac aaatataaag gactcggcga accgtttgta tttgttctga
     4141 tgggcctgct tcttttcggc ggcgcatttt ataccgcaac cggaaccatc acccttggcg
     4201 cggtctggcc cgcactggcc tgcggctgcc tggtgactgc gatcctgctg gtgaataatc
     4261 tgcgcgacat tcaaatggat ggccaggccg gtttcacgac actgccgata cggataggcg
     4321 cgacgcgaac aaaaaacctc tacgccgcgc tcgttgtcac ggctcttata gcaacctgcg
     4381 ccgccggcat attcctcccg ctgttgagcc tgccgctcgc atggcggcgg attcattccg
     4441 ttcagaccgc caccgagcca agtcatgatc ttgccgacgc tccgcagcag accgccgcat
     4501 tttatctgat cttcggcgta ctgctggcag ccgggctg
//
LOCUS       DAOPZO010000139         4521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_24671_length_4521_cov_7.882221, whole genome shotgun sequence.
ACCESSION   DAOPZO010000139 DAOPZO010000000
VERSION     DAOPZO010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24671_length_4521_cov_7.882221"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PKI68_08075"
     CDS             <1..208
                     /locus_tag="PKI68_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53687.1"
                     /translation="CASAAALRLKSPVHADTEDKAGRVSFSSKTRSALGSDIVKTVDM
                     EDLLPNRRMYQSGFSPRITRINTN"
     gene            complement(256..918)
                     /locus_tag="PKI68_08080"
     CDS             complement(256..918)
                     /locus_tag="PKI68_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53688.1"
                     /translation="MKKLIRTVFGLLALTAVIFISGAIFYGTKTVHDLLGENKKLKQS
                     LAVLTQEDQIGYAKVVGQETRGGKLYTIVRFVETARDDRLKTVLEKEYEIEGDTVHFD
                     ALVVTFPGQAVMDGKERSLYLWRRIYGEKQAPAFGFAIEKDGVPPARYNDLLGRLHFS
                     ERKLFWTAIWDLANNPEALQQYGIKAVYGNAVYQKLRPGLIYVFKISPTGQLYPETVP
                     DL"
     gene            1061..1708
                     /gene="tmk"
                     /locus_tag="PKI68_08085"
     CDS             1061..1708
                     /gene="tmk"
                     /locus_tag="PKI68_08085"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNX53689.1"
                     /translation="MKGKFITLEGPEGSGKSTQAKTLIQRLAERGIDAVYTREPGGTA
                     LGEAIRNILQHNQAGEAPCERAELLLFEASRNQLVEKVIRPNLEKGIWVICDRFMDST
                     TAYQGYGRGLPVEEIQAINRFTVNGVSPDLTLLLDLEVATGFERIAQRYAVRGETADR
                     FEQEERSFHEKVRAGYLELANAEPARFRIVNAAQAPDAVAASIWDTVTDVFRQSI"
     gene            1686..>2605
                     /locus_tag="PKI68_08090"
     CDS             1686..>2605
                     /locus_tag="PKI68_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53690.1"
                     /translation="MSSGSQFSSAWTGIETGWRNGQLAHAYLLQGAPHGASLRFAEKL
                     LNLVFNNHPQVQTRAHPDIVWLEPQSKSRQITIDEIRDLIRQLSQTSFAGGWKAGVIL
                     CADRMTDQAANALLKTLEEPPSGTLLILLTDEPQALLPTISSRCQRISLSDEDEEVSR
                     HWKDPLLDILGELPPASVTEAGLFTGRITGILDELKKEFEAEESGRLPEDLSAKEAKD
                     LLAARSTARLIESRTEILRTMLQWQRDVLMLVLEQDEASLHFQDDIEPLKRQAMLCTR
                     ADALRRIAAIEETGRQFDRNLPAELVFNNF"
     assembly_gap    2606..2705
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2706..3156)
                     /gene="aroQ"
                     /locus_tag="PKI68_08095"
     CDS             complement(<2706..3156)
                     /gene="aroQ"
                     /locus_tag="PKI68_08095"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNX53691.1"
                     /translation="MKTLKILVLNGPNLNLLGTREPEIYGTETLASIAQDMNACAEKL
                     GDVVLEFRQSNAESDLITWIGDSRGKFDGIIINPAAYTHTSLGLGDAVKAVAKEVPAI
                     EVHLSNTHAREEIRHKSLIAPGCIGQIMGFKGYGYILALKAMVKHLRG"
     gene            complement(3359..4288)
                     /locus_tag="PKI68_08100"
     CDS             complement(3359..4288)
                     /locus_tag="PKI68_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNX53692.1"
                     /translation="MTMSCMFKIIKRCLLSSLPFLAMQIFAADWRAEVIPLNGTELNH
                     PFGIVRGPDRCIYFCEVEGNVVRKIETNGTITTVAAGFNNPHELRFDKNGQLFVADTG
                     NHRIVRVDLQTGRLTSITSKIFKTPISIQADANGDIFVCEIGRNSIVRVNPNNGLVTP
                     VMGDELEKNLKGPRSIDFDRSGNLWIVLRGGNKVLKFDPAARTLAEMAGTGETGFTGD
                     GGPALLAALNGPKGISISPDGQRIFLADTENHVIRMIDLKTGNIELIWGTGKKGAGVN
                     ELSRPHALFAEVDGSLLVSDSDNNRLLVLRPSE"
     gene            complement(4337..>4521)
                     /locus_tag="PKI68_08105"
     CDS             complement(4337..>4521)
                     /locus_tag="PKI68_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53693.1"
                     /translation="NFPALLFYPPVSWRLTRHFSSQRQHNLLHIGVGWFPRQAPGHSA
                     TSLNVELFAGSTGGWR"
BASE COUNT         1001 a         1216 c         1167 g         1037 t
ORIGIN      
        1 ctgcgcttct gccgcagcgt tgcggttgaa atcacccgtt cacgctgata ccgaagacaa
       61 ggccggcagg gttagttttt cttctaaaac ccgctcggct ctcggttcag atattgtaaa
      121 aactgtcgac atggaagact tattaccgaa tcgccgaatg tatcaaagtg gattttcccc
      181 acgaataact cgaataaaca cgaattaaga agccttccat tcgagaaaat tcgtgcagat
      241 tcatgggcgg ttcttttaca aatccggaac ggtttcggga tagagctggc cggtcgggct
      301 gattttgaag acgtaaatca ggccgggccg gagtttttga taaaccgcat tgccatagac
      361 ggctttaatg ccgtactgct gaagtgcttc tggattgttg gccagatccc agatggcggt
      421 ccagaatagc ttcctctcgg aaaaatgcag ccgtccaagc aggtcgttgt agcgggcggg
      481 cggtacaccg tctttttcaa tggcgaagcc gaatgccggc gcctgttttt caccgtagat
      541 gcgccgccag aggtaaagcg agcgctcttt tccgtccatg accgcctgac cgggaaaggt
      601 gacgaccagc gcgtcgaagt gcacggtatc gccttcaatc tcatattcct tttcgagaac
      661 ggttttcagc cggtcgtctc gggcggtttc gacaaaacgg acaatggtgt aaagttttcc
      721 gccgcgggtt tcctgcccga cgacctttgc gtagccgatc tggtcttcct gcgtcagcac
      781 cgccagcgac tgtttgagtt ttttattttc gccaagcagg tcatggacgg tttttgttcc
      841 gtaaaaaatc gcaccggaaa taaaaatgac cgccgtcagc gccagcagtc cgaaaaccgt
      901 tcgaatcagt tttttcatga gtaacaatag tcatatttcc cacgaatctg cacgaatatt
      961 cacgaattcg gcttctgtat tcgtgttaat tcgtgtgatt agcgggcggg ctgttttttt
     1021 tcgtggtttc tcgtgttttt cgtggcaatc ttcccggctc atgaaaggca aatttataac
     1081 tctggaaggg ccggaaggtt ccggaaaatc gacgcaggcg aagacgctga tccagcgtct
     1141 ggccgagcgc ggcattgatg ccgtttacac ccgcgaaccg ggcggcacgg cgctgggcga
     1201 agcgatccgc aatattctcc agcacaatca ggcgggtgaa gcgccctgcg agcgcgccga
     1261 actgctcctg tttgaagcca gccgcaatca gctggtggaa aaagtaatcc gcccgaatct
     1321 ggaaaaaggc atctgggtca tctgtgaccg tttcatggac tcaacaaccg cctatcaggg
     1381 ctatggacgc ggcctgcccg tcgaagaaat ccaggcgatc aaccgcttta cggtcaatgg
     1441 cgtttcgccc gaccttaccc ttctgcttga tcttgaagtc gccacgggct ttgagcggat
     1501 tgcgcagcgt tatgccgtcc gcggcgaaac ggcggatcgc ttcgaacagg aagaacgttc
     1561 gttccacgag aaagtgcgcg ccgggtattt ggaactggca aacgccgaac cggcgcgctt
     1621 ccgtatcgtc aatgccgcgc aagctcctga cgccgttgcg gcttcaatct gggataccgt
     1681 taccgatgtc ttccggcagt caatttagtt ctgcctggac aggcattgaa accggctggc
     1741 ggaacggtca gctggcgcac gcctatctat tgcagggcgc accgcacggc gcctcgctgc
     1801 gcttcgccga aaaactgctc aatctggttt tcaataatca cccgcaggtt caaacccgcg
     1861 cccatcccga catcgtctgg ctggaaccgc agagcaaaag ccggcagatt acaatcgatg
     1921 aaatacgcga cctgatccgg cagctttcgc aaacctcgtt cgcaggcggc tggaaagccg
     1981 gcgtcatcct ttgtgccgac cgtatgaccg atcaggcggc caatgccctg ctcaaaacac
     2041 tggaagagcc tccgtccgga actctgctca ttctgcttac tgacgaaccg caggcgctgc
     2101 tgccgacgat ttcgtcgcgc tgccagcgga tttcactctc cgatgaagac gaagaggttt
     2161 ccagacactg gaaagacccg ctgctcgata ttctcggcga actgccgccg gcaagcgtaa
     2221 cagaagccgg cctgttcacc ggacggatca ccggaattct cgatgaactg aaaaaagaat
     2281 ttgaagccga ggagtccggc cgtctgccgg aagatctgtc ggccaaagaa gccaaagatc
     2341 tgctcgccgc ccggtcgact gcacggctga ttgaatcgcg aaccgaaatc ctgcgaacca
     2401 tgctccaatg gcagcgcgac gtgctgatgc tggtattgga acaggacgag gcctctttgc
     2461 actttcagga tgacattgag ccgctgaaac ggcaggcgat gctctgcact cgcgccgacg
     2521 ccttgcggcg cattgccgct atcgaggaaa caggacggca gttcgaccgg aatctgcccg
     2581 ccgaactggt gttcaacaat ttcttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnagccg cgcaggtgtt taaccattgc tttgagggcg aggatgtagc cgtagccctt
     2761 gaaccccatg atctgtccga tacagccggg agcaatcagg cttttatgcc ggatttcttc
     2821 gcgggcgtgc gtgttgctca aatggacttc gatagccggc acttctttgg cgacggcttt
     2881 aaccgcgtca cccagcccca gacttgtatg agtataggcg gccggattga tgatgatgcc
     2941 gtcaaatttt ccgcggctgt cgccgatcca ggtaatgaga tcgctttcgg cgttgctctg
     3001 ccggaattcc agcacgacat cgcccagctt ttccgcacag gcgttcatat cctgcgcaat
     3061 cgaagcgagg gtttccgtgc cgtagatttc cggctcgcgc gtaccgagaa ggttcaggtt
     3121 cggaccgttc agaacaagaa ttttgagcgt tttcatggcg gaagttctac catagaaccc
     3181 gcagatagca cggattttct ggaagaacgt ggcaaggaac ttacgctgcc gtaagtctgt
     3241 ttaaatatgg ccgcggggcc cgggtttgaa tcggaccccg gctaaatcac cccggaaata
     3301 attatggtac cggtttggtg caaaccactt gcaccgctat ttgatttccg ccttaagttt
     3361 attccgacgg ccgcagcaca agcaggcggt tattatcgct gtcactgacc agaagcgaac
     3421 cgtcgacttc agcaaacagc gcatgaggac gcgagagctc gttgacgccg gcgccctttt
     3481 tacctgttcc ccatatcagt tcaatattgc cggttttcaa gtcaatcatc cgtatgacat
     3541 ggttctcggt atcggcgagg aaaatccgct gtccatccgg ggaaatggag atacctttgg
     3601 gtccgttcag tgcggccagc agcgccggcc cgccatcgcc tgtaaatccg gtttccccag
     3661 tccccgccat ttcagcgagt gttctggcgg ccggatcaaa ttttaaaacc ttattgcctc
     3721 cacgcagcac gatccacaaa ttgccgctac ggtcaaaatc gatactgcga gggcctttga
     3781 gatttttttc taattcatcc cccatgaccg gtgtaaccaa gccgttgttc ggattcaccc
     3841 ggacaatact gtttctgccg atttcacaaa cgaaaatgtc cccgttggca tccgcttgaa
     3901 tactgatcgg cgttttaaat attttactcg tgatggatgt aagcctgccg gtctgcaaat
     3961 caaccctgac gatccggtgg ttaccggtat cagccacgaa gagctgcccg tttttatcaa
     4021 accggagttc gtgggggtta ttgaatccgg cagcaaccgt ggtgattgtt ccgtttgttt
     4081 cgattttccg gacaacatta ccttcaactt cacaaaagta gatgcaccgg tccggccccc
     4141 tgacgatgcc aaaaggatgg ttaagttcag ttccgttgag cgggatcact tccgcccgcc
     4201 aatcagcggc aaaaatctgc atcgctaaaa acggcaagga agacagaaga catcgtttta
     4261 taattttgaa catgcacgac attgtcattt tctcgctccg ttttttccag gcacacacat
     4321 tatatgctcc tgtccgttac cgccatccgc ctgttgaacc ggcaaacagt tccacgttta
     4381 acgacgttgc tgaatgtccc ggggcctgac gggggaacca accgaccccg atatgtaaca
     4441 ggttgtgctg ccgttgagaa ctgaaatggc gggttaaccg ccaggaaaca gggggataaa
     4501 acaaaagagc cggaaaattc a
//
LOCUS       DAOPZO010000140         4419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_25515_length_4419_cov_8.712649, whole genome shotgun sequence.
ACCESSION   DAOPZO010000140 DAOPZO010000000
VERSION     DAOPZO010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25515_length_4419_cov_8.712649"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PKI68_08110"
     CDS             <1..549
                     /locus_tag="PKI68_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine-cytidine kinase"
                     /protein_id="HNX53694.1"
                     /translation="TTVAQELAELAGHASLMSQDNYYISLPDDVDAKNWNFDDPAVID
                     LEHLARDLAALKRGETVEGPRYIFSTHKRAPDTVTLKPAPLIIVEGLFLFLPESLRDV
                     FDLKIFIDVPLEVCLERRVARDVAERGRTAETIRQRWAEQVEPMYRKYVEPTRTCADL
                     LLVPAERGTPERKQQIAGILNR"
     gene            715..1302
                     /locus_tag="PKI68_08115"
     CDS             715..1302
                     /locus_tag="PKI68_08115"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNX53695.1"
                     /translation="MNTFWLCFVPLFVAVDAVGVLPMFLSLTDGLDAKRLRTVIIQSV
                     LTAATVAVVFVLAGPILLRFMGITVPDFMLAGGLLLLAISLNSLLTGEKKQREVDISS
                     LGAVPIGIPLITGPAVLTTCILLATTHGKVITSIAVILNIAIAGLIFGFAEPITKRLG
                     RTGSKAVSKVASMLLAAIAVMLMRKGLFAMLAAAQ"
     gene            complement(1307..2701)
                     /locus_tag="PKI68_08120"
     CDS             complement(1307..2701)
                     /locus_tag="PKI68_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53696.1"
                     /translation="MSEETSAPQNVSRPDEDVLLDLNFVPQWAKKAPAANHYASDDRS
                     EYRGPSRDRHDGPRRERSDRRDGPSRTARPPRERSSGERPNALSGAGRPPPREPRGER
                     AAPEPRREAVVRPERIELPVEIKFLPEQQWLASMVRQIHHSKRAYPLMDLANLFLADP
                     KGHLVKIEIRPGSDGFKLYQGKRSKMVATSREALVHQLLDEHMDDFFKKEEIEVEQPT
                     GVFPCIAKVGDLLVGPPNHHSYAARLQEIYNSRFSGMPFERFKEKIQTVRDEELIAKW
                     KQESSKKTVYRLKDAPEGEVKDFTLAQAEDYIRTRIADSEIEEVSRAVLPSALARKIK
                     DYNLIRMVREAWESESRFPLSVSFALRAAFRHLHLQTFKAGKNINFVTAVKPDPIKPE
                     KAVEHIRLVLEYLAEHPGSTREQLVEGLRPGAEKDSDAAREILNPLRWLVEKGHIIEF
                     FNGTLAVPSHRKRR"
     assembly_gap    2766..2865
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2866..>4419
                     /locus_tag="PKI68_08125"
     CDS             <2866..>4419
                     /locus_tag="PKI68_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53697.1"
                     /translation="VPPEELEKYSSAITLSDMEIFVFPELLYALLLANIMSPRIWKWR
                     DDPWFAKIDKMSPRKKIDRLKQYIIDHYEFNLDLDTWGLTDQQTELKRFTPFMDAKII
                     SGSNALFGYEGDRHYFDLDIRRHFGLEKYSDSVIPYWKTETVEAMDAFNRREGYRTGA
                     GECVSLSTLYAAALFIVCGIPLEDIFLMATPLHSQNFVAVGDGILTNNRRIVTKNMWF
                     NGTELTDKAQRALRHEQITMVFNNTGYVHCVYPETTMDPAHYERFRQQIGEYLSTEIT
                     FEILANFLRQNSRFQKCFQICHDFHGKKRWITAERAYAYEHSAPYKIGDSTRSQLLEE
                     IDNDEFYCEPIPGRICLPRLEAFFKNNPVQHYDEASMAELGAKLECSSSGKNEMLAEL
                     AKFVHLEPEFPDVTNKVSAPWKKLEITPGMTREEITAYVESVRAENRSADLAFYALRD
                     LSRTDWAPFLKAALERNPVCIEATKAMSDEELLQLLDSMNPESIYDGPRLAQPDEVWN
                     YKTGDGLEKA"
BASE COUNT          898 a         1318 c         1212 g          891 t
ORIGIN      
        1 accacggtcg cgcaggagct ggctgaactg gccggacacg ccagtctgat gtcgcaggac
       61 aattattaca tcagcctgcc cgatgatgtg gatgccaaga actggaactt tgacgatccg
      121 gcggtgattg atctcgaaca cctcgcccga gacctcgccg cgctcaaacg cggcgaaacc
      181 gtcgaaggcc cacgctatat tttttcaacc cacaaacgcg cgccggacac cgtcacgctt
      241 aaaccggcac cgctcatcat cgttgaaggg ctttttctct tcctgcccga atctctgcgc
      301 gatgttttcg atctgaaaat cttcatcgat gtgccgctgg aagtctgcct ggaacgccgc
      361 gtcgcccgcg acgttgctga acgcggccgc accgccgaaa ccatccgtca gcgctgggcc
      421 gagcaggtcg agccgatgta ccggaaatat gtcgagccga cccgcacctg cgccgatctg
      481 ctgctggttc ccgcagaacg cggcacgccg gaacgcaaac agcagattgc cggcatcctg
      541 aaccgttaat tagaacaagg ccggctggaa gccggcgata cagaattaac acaaggccgg
      601 caggatgccg gcgatgcatc tgacgtaccg ccggcttcca gccggccttg gccttgtaaa
      661 agcgaaaccg cgccgcgctt gccgccgcac tccggaacat gcgatagtcc gggcatgaac
      721 acgttctggc tttgcttcgt tccgctgttt gtcgcagtag atgccgtcgg ggtcctgccg
      781 atgtttctct ctctgacgga cgggctagat gctaagcgct tgcgcaccgt catcattcag
      841 tcggttctga cggccgccac ggttgcggtg gtgttcgttc tcgccgggcc gatcctgctg
      901 cggtttatgg ggattaccgt tccggacttt atgctggcgg gcggtctgct gctgctggcg
      961 atttcgctga acagcctgct gaccggcgag aaaaaacagc gggaggttga tatttcgagc
     1021 ctcggtgcag tgccgatcgg cattccgctg atcaccggcc cggcggtgct gacaacctgc
     1081 attctgctgg ccaccacgca cggcaaggtc atcacgtcga tagccgttat cctcaatatc
     1141 gccatcgccg gtctgatttt cggctttgcg gaaccgatta caaagcggct cggacgcacc
     1201 ggctcgaagg cggtctctaa agttgccagt atgctgctgg cagcgatcgc ggtgatgctg
     1261 atgcgtaaag gtctcttcgc aatgctcgcc gccgcgcagt aagcggttag cggcgtttgc
     1321 ggtgcgacgg tacagccagc gtgccgttga agaattcgat gatgtgccct ttttcaacca
     1381 gccagcgcag cggattgaga atttcacggg cagcatcgga atccttttcc gctccgggcc
     1441 gcagtccttc gaccagctgt tcgcgggtcg agcccggatg ttcagccaga tattccagca
     1501 ccaggcggat atgttcaacc gccttttcgg gcttgatcgg atcgggtttg accgccgtca
     1561 cgaagttgat gtttttgccg gctttgaagg tttgcagatg caggtgacgg aaggcggcac
     1621 gcagggcaaa ggaaacggac agagggaaac ggctttcgct ttcccacgct tcgcgcacca
     1681 tgcggatgag gttgtagtct ttgattttgc gggccagcgc ggacggcaga acggcgcgtg
     1741 aaacttcttc gatctccgag tcggcgatcc gcgtgcggat ataatcctcg gcctgggcca
     1801 gagtgaaatc tttcacttcg ccttcgggcg cgtctttcag ccggtagacc gttttcttcg
     1861 acgattcctg tttccatttc gcaatcagtt cttcgtcgcg aacggtctgg atcttttcct
     1921 taaaccgctc aaagggcatt cccgaaaagc gtgagttgta aatttcctgc aggcgcgcgg
     1981 catagctgtg gtgattgggc gggccgacca gcaggtcgcc gactttggcg atgcacggga
     2041 aaacaccggt cggctgttcg acttcgattt cttctttttt gaagaagtcg tccatgtgct
     2101 cgtcgagcag ctgatgaacc agcgcttcgc ggctggtcgc caccattttg gaccgtttgc
     2161 cttgatagag tttaaacccg tctgaaccgg gcctgatttc gattttgaca agatgccctt
     2221 tgggatcggc caagaacagg ttggccagat ccatgagcgg ataggcgcgt ttgctgtgat
     2281 gaatctgtcg caccatggac gccagccact gctgctccgg caggaatttg atttcaaccg
     2341 gcagctcaat gcgttcgggc cgcacaacgg cttcccggcg cggttcgggt gctgcgcgct
     2401 caccgcgcgg ttcgcgcggc ggcggccggc cagccccgct caaagcattc ggccgttctc
     2461 ctgaagagcg ctcgcgcggc ggacgggccg tacgggacgg accgtcccgg cggtcactgc
     2521 gctcgcggcg cgggccgtca tgccggtcgc gcgagggccc gcggtactcg gaacggtcgt
     2581 cggaggcgta gtggttggct gccggcgctt tttttgccca ttgcggcaca aaattgaggt
     2641 cgaggagaac atcctcgtcg ggccgggaca cattctgcgg agcgcttgtt tcttcagaca
     2701 tggtaaaacc ttttaaattg agcgggcatt agtatacgct ttccggtgtt tggaaaagga
     2761 aaaacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtacc gccggaagaa
     2881 ctggaaaaat actcttcagc catcacactt tcagacatgg agattttcgt tttccccgaa
     2941 ctgctctatg ccctgctgct ggccaatatc atgagtccgc gcatctggaa gtggcgcgac
     3001 gacccgtggt tcgccaagat cgacaagatg agcccgcgca agaagatcga ccggctcaag
     3061 cagtacatta tcgaccatta cgagttcaac ctcgacctcg acacctgggg gctgaccgac
     3121 cagcagaccg aactgaagcg gttcactccg ttcatggacg ccaagatcat cagcggcagc
     3181 aacgccctgt tcggttatga aggcgaccgg cactatttcg acctcgatat ccgccgccac
     3241 ttcggcctcg aaaaatacag cgacagtgta attccgtact ggaaaaccga aaccgtcgaa
     3301 gccatggacg cctttaaccg gcgcgaaggc taccggaccg gcgcaggcga gtgcgtttcg
     3361 ctttccaccc tttacgccgc cgcgctgttc atcgtctgcg gcattccgct cgaagatatt
     3421 ttcctgatgg ccacgccgct gcattcgcag aacttcgtgg cggtcggcga cggcatcctc
     3481 accaacaacc ggcgcatcgt caccaaaaac atgtggttta acggcacgga gctgaccgac
     3541 aaggcgcagc gcgccctgcg ccacgaacag atcaccatgg tgttcaacaa caccggttac
     3601 gtgcactgcg tctatcccga aaccaccatg gatcccgcgc actacgagcg gttccggcaa
     3661 cagatcggcg aatatctctc caccgaaatc acattcgaaa tcctcgccaa ctttctgcgg
     3721 cagaattccc gtttccagaa atgctttcag atctgccacg attttcacgg caagaagcgc
     3781 tggatcacgg ccgaacgcgc ctatgcctac gaacacagcg ccccctacaa aatcggcgac
     3841 agcacccgca gccagctgct cgaagaaatt gataatgacg agttttactg cgaaccgatc
     3901 cccggccgca tctgcctgcc gcggctggaa gcgtttttca aaaacaatcc cgtccagcat
     3961 tacgacgaag ccagcatggc ggaactcggc gcgaaactcg aatgcagcag ctccggcaaa
     4021 aacgaaatgc tcgccgaact ggcgaagttc gtccacctcg aacccgaatt cccggacgtt
     4081 acaaacaaag tttcagcgcc gtggaaaaaa ctcgaaatca cgcccggcat gacccgcgaa
     4141 gaaatcaccg cctatgtcga atccgtgcgc gccgaaaacc gctccgccga cctcgccttc
     4201 tacgccctgc gcgacctcag ccgcaccgac tgggcgccgt ttctcaaagc ggccctcgaa
     4261 cgcaatcccg tctgcatcga agcaaccaaa gcgatgagcg acgaagagct gcttcagctg
     4321 ctggactcaa tgaatccgga atccatttac gacggcccgc gcctcgccca gcccgacgaa
     4381 gtctggaact acaaaaccgg cgacggtctc gaaaaagcc
//
LOCUS       DAOPZO010000141         4414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_25553_length_4414_cov_8.827713, whole genome shotgun sequence.
ACCESSION   DAOPZO010000141 DAOPZO010000000
VERSION     DAOPZO010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25553_length_4414_cov_8.827713"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /gene="pyrH"
                     /locus_tag="PKI68_08130"
     CDS             complement(<1..244)
                     /gene="pyrH"
                     /locus_tag="PKI68_08130"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNX53698.1"
                     /translation="MKYKRILLKLSGEALQNREKGLSIDPDILASIGRQFKQLIDAGA
                     EIAVVVGGGNIFRGLAGEAGGTDRTTGDSMGMLATVI"
     gene            complement(316..1467)
                     /locus_tag="PKI68_08135"
     CDS             complement(316..1467)
                     /locus_tag="PKI68_08135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX53699.1"
                     /translation="MRKSRFRWIFSVLLVSAIFFGCGRKPGEKLYHEALAEWNDGNLA
                     RARALLEKSIRRRAGSAENAEAYNRLGVLLWEMGNSKDAVDAFNESIRMDAGQYDVLC
                     NLGVALSSQNDFTAAERAFREASLIKADDPRPLAFAGVSYARNQRWNDAANNLNRALG
                     RTPKDPCLQTALALAELHTTGAESALKRLQAVAKQYPDYAPAFFNTASIYRYWVKNQT
                     EAKRWFELYLNKVPADSPFAAQARAQLQAIAAGDNGEKLTFTPPRSPNRIAADKNFEK
                     ALEYQKKNDFDNAIKWYIKAVEEDDTYEQAFYNLGLAYYAANRIGLASDAFARAVQLN
                     PAFTSARYNCALAEYRLGHNDRALRELEMVISQQPKYQPAIDLRARIKK"
     gene            complement(1464..1856)
                     /locus_tag="PKI68_08140"
     CDS             complement(1464..1856)
                     /locus_tag="PKI68_08140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNX53700.1"
                     /translation="MNEFTVQSKSRTEFIDITVAVQQFVSGKNIKDGLITVFVPHTTA
                     GVTINENADPDVTADLETVLDRMVPWNGGYRHSEGNSAAHVKASLTGSSAQVIVKDGR
                     LRLGTWQSIYFCEFDGPRTRQVWLKEIS"
     gene            1926..2876
                     /locus_tag="PKI68_08145"
     CDS             1926..2876
                     /locus_tag="PKI68_08145"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HNX53701.1"
                     /translation="MKTLYPLRFNPVYKDYIWGGSRIPKLYNRDLPDGIYAESWEIST
                     HPDGTTQIANGPLAGKTLRDLLPEHKTALLGTGIKGDDFPLLIKLIDSREILSVQVHP
                     NNTNAAAVNGEPKTEMWYFLEGDGTAQIYCGLKPGIGKTEFLNALENKTFFSILKTIP
                     AQKGEAVFVPGGCVHSIGVGCLILEIQQNSNTTYRIYDWDRVDASGKGRELHIDKALK
                     VINWNDTSDPLCKISGTTIQKCDFFRLDRFELKAAEDFPMDGKSFHALFIAEGSGTIA
                     WNGGQEKLSPGQSWLVPAALGRYTVCPAGSGLTVLCTTVP"
     gene            complement(2944..4179)
                     /locus_tag="PKI68_08150"
     CDS             complement(2944..4179)
                     /locus_tag="PKI68_08150"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNX53702.1"
                     /translation="MIKKSAIQFLALTLILAICSCAPVTIDTVPTGAAVYSADGQTQL
                     GTTPYNTSIFVSDKNFTVRKDRYFDEPVNLNFDSARNIELKLRPTPVLVYSKPDAQIY
                     PAGSETALGKTPMKVPVYAKERTYTLKAADYYDQDIKVSADSIDPLVVKLERRPIVTL
                     SAVPSGVEVYENGSLIGTAPVRQEILASRTFELRKAGYFTKSVTLTGAPPYEMTAELK
                     PFPVITVAATPADAQIYRAGAVAGKAPFKLAVGEKTVLEVRADRYYPQSVTLTPESPA
                     QVNVALKAMPYVTINSEPAGAELFIGGKSSGITPVELLIEKDTVVELRKEGFIAKSAT
                     LTGADKQVTVTLEAVPPPPPAVQAAAEPGAKASAVTAPEVQAAKNAPAKSNKLLWAGV
                     IIVAAAIAGFFIIKRKKQQ"
BASE COUNT          917 a         1167 c         1295 g         1035 t
ORIGIN      
        1 tgatgacggt ggcgagcatg cccatggagt cgccggtggt gcggtcggtt ccgccggctt
       61 cgcctgcgag accgcggaag atatttccgc cgccgacgac tacggcgatt tccgctccgg
      121 cgtcgatgag ctgtttaaac tgccggccga tggaggcgag gatgtcggga tcaatgctca
      181 ggcctttttc tctgttctgg agtgcttcgc cgctgagttt gagcagaatg cgcttatact
      241 tcataaattt cctctcgaat gctggggcac cataaaaaat ccgagtccaa gtgtaaattt
      301 ccaaaccgcg gaaaattatt ttttgatgcg ggcccgcagg tcaatagccg gctgatattt
      361 aggctgctgg ctgatcacca tttcaagttc gcgtaacgca cggtcgttat gtccgaggcg
      421 gtattccgcc agcgcgcagt tgtaacgtgc cgaagtgaag gccggattga gctgtaccgc
      481 ccgtgcgaag gcatccgacg ccagcccgat gcggttggcg gcgtagtaag ccaggccgag
      541 gttgtagaac gcctgttcgt aggtgtcatc ctcctcaacg gctttgatgt accatttgat
      601 cgcattatca aagtcatttt tcttctggta ctccaacgcc ttctcgaaat ttttgtcggc
      661 ggcaatgcgg ttcgggctgc gcggcggcgt aaaggtcagt ttttcaccgt tgtcgccggc
      721 ggcaatggcc tgcagctggg cgcgggcctg ggctgcgaag ggactgtcgg cgggaacttt
      781 gtttagataa agctcgaacc agcgtttggc ttccgtctgg tttttgaccc agtagcggta
      841 gatggacgct gtgttgaaaa acgccggagc ataatcagga tactgttttg caacggcctg
      901 cagccgtttc agtgcggatt ctgcaccggt ggtgtgcagt tcagccagag ccagagcggt
      961 ttgcagacaa gggtctttcg gagtgcgccc gagcgcccgg ttcagattgt ttgccgcgtc
     1021 gttccagcgc tgatttctcg cgtagctcac gccggcgaaa gccagcggac gcgggtcgtc
     1081 ggctttaatc aaagaggctt cgcggaaagc gcgttcggcg gcggtgaaat cattctgtga
     1141 gctgagcgcc acgccgaggt tgcagagcac atcgtactgc ccggcatcca tgcggatgct
     1201 ctcattaaat gcatccaccg cgtcttttga atttcccatt tcccagagca gtacgccgag
     1261 ccggttgtag gcttccgcgt tttccgcgct gcccgcccgg cggcggattg atttttcgag
     1321 cagggcgcgg gcgcgggcca gattgccgtc gttccattcg gccagcgcct cgtggtaaag
     1381 cttttcgccg ggcttgcgtc cgcagccgaa aaaaatcgcg ctgaccagca gtacggaaaa
     1441 aatccaacga aaccggctct ttctcatgaa atctccttca accagacctg ccgcgtgcgc
     1501 gggccgtcaa attcacaaaa atagatgctc tgccaggtgc caagccggag cctgccgtct
     1561 ttgacaatca cctgcgccga cgagcccgtc aggctggctt tgacgtgcgc agcgctattg
     1621 ccttcactgt ggcgatagcc gccgttccac ggcaccatcc ggtcgagaac cgtttccaaa
     1681 tcagctgtca cgtcggggtc ggcgttttcg ttgatggtga cgccggcggt ggtgtgcggg
     1741 acaaaaaccg tgatgagtcc gtctttgatg tttttgccgg aaacgaactg ctgaacggcg
     1801 acagtgatgt cgataaattc ggtgcgggat tttgactgga cggtaaactc attcatgcct
     1861 aacctgcact ctaatcatcc ggctttttga agccagataa tttgtgcaaa aacaggaatc
     1921 cggctatgaa aacgctctat cctctccgct tcaaccccgt ttataaagac tatatctggg
     1981 gcggcagccg gattccgaaa ctgtacaacc gcgatctgcc cgacgggatc tatgctgagt
     2041 cctgggagat ttcaacccat ccggacggga ccacgcagat tgccaacggc ccgctggccg
     2101 gcaagaccct ccgcgacctt cttccggagc ataaaaccgc actgctcgga accggaatta
     2161 aaggcgatga ctttccgctc ctgatcaagc tgattgattc ccgcgaaatc ctgagcgtac
     2221 aggttcaccc gaataacaca aacgccgctg cggtcaacgg ggagccgaaa accgaaatgt
     2281 ggtattttct tgaaggcgac ggaaccgcgc agatttactg cggactcaag ccgggcattg
     2341 gcaagacaga gtttctgaac gcactggaga ataagacgtt tttcagtatt ctgaaaacca
     2401 ttccggcgca gaagggtgag gcggtttttg ttcccggcgg ctgcgttcac tccatcggtg
     2461 tcggctgcct gattcttgaa attcagcaga actccaatac cacctaccgc atatatgact
     2521 gggatcgcgt ggatgcctcc ggcaagggac gcgagctgca tatcgataaa gcgctgaaag
     2581 tcatcaactg gaacgatacc agcgatccgc tttgcaaaat cagcggaaca accattcaga
     2641 aatgcgactt tttccggctc gaccgctttg agctgaaagc ggcggaagat tttccgatgg
     2701 atggaaaatc gttccatgcg ctgtttattg ctgaaggatc gggaaccatc gcatggaacg
     2761 gcggacagga aaagctgtcg cccggccagt cgtggctggt tcctgctgcg ctcggccgct
     2821 acacggtttg cccggccggt tccggattga ccgtgttgtg tacgacggtt ccgtaacctc
     2881 cgcaaactcc ggacacaaaa aaagagccgc ataagcggct cttttttaat gccttgaagc
     2941 ggcttactgc tgttttttgc gtttgatgat aaagaatccg gcaattgcgg ccgcaacgat
     3001 aataacgccg gcccacagca gtttgtttga tttggccggc gcattcttcg cagcctgtac
     3061 ttccggagcc gtaacagcgg aggctttagc cccgggttca gctgcagcct gaacagccgg
     3121 cggcggaggc ggcacggctt cgagggttac agtaacctgc ttatcggcac cggtgagggt
     3181 ggcggacttg gctatgaatc cttctttgcg cagttcaacg acagtatctt tttcaatcag
     3241 cagttcaacc ggagtgattc cggaagattt tccgccgatg aacagttcag cgccggcagg
     3301 ttcgctgtta atcgtgacat aaggcatggc tttcagtgcg acattgacct gtgccggcga
     3361 ttcgggcgtg agcgttacgc tctggggata atagcggtct gcgcggactt ccagcacggt
     3421 cttttcgccg acggcgagtt tgaacggcgc tttgccggca acagctcctg cgcggtagat
     3481 ttgcgcatca gccggcgttg cggcaacagt gatgaccggg aaaggtttga gttcagcggt
     3541 catttcatac ggaggtgcgc cggtcagcgt gacgctcttg gtaaagtagc cggctttacg
     3601 caattcgaac gtgcggctgg ccaggatttc ctgtctgacc ggagcggttc cgatgaggct
     3661 gccgttttcg taaacttcaa caccggacgg aacggcggaa agggtgacaa tcggccggcg
     3721 ctccagtttg acgacgagcg ggtcaatgga atcagcgctg actttaatgt cctgatcgta
     3781 atagtctgcg gccttcagcg tgtaggtgcg ttccttggca taaacgggca ctttcatcgg
     3841 tgtttttccg agggcggttt cagaacccgc cggataaatc tgggcgtcag gtttgctgta
     3901 aaccagtacc ggtgtcgggc ggagtttcag ttcaatgttg cgcgctgagt cgaaattgag
     3961 attaacgggt tcgtcgaagt agcggtcttt gcgaacggtg aagtttttgt cgctgacgaa
     4021 gatgctggtg ttgtacggcg tcgtgcccag ctgtgtctgg ccgtcggcgc tgtatactgc
     4081 cgcacccgtc ggaacggtgt cgatagtaac aggagcgcaa ctgcaaattg cgaggataag
     4141 ggtcagtgcg agaaactgaa tagctgattt tttaatcatc ataggctcct tggataggtt
     4201 gcaaacacgg tgatttatac cgcagtgaaa ggttttaagt caaacgggct ggccgcttat
     4261 tttgtcggtg agggtttgat ctccggcctt ttaacggcgt tgaccgccag cgccagcgcc
     4321 atcagcgcgc cgccgcccag caaacgcggc cagtcggccg tttcgccgaa aaagagcacc
     4381 gaaacgagaa taccgagcgg tattttcagg ttat
//
LOCUS       DAOPZO010000142         4353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_26105_length_4353_cov_8.488367, whole genome shotgun sequence.
ACCESSION   DAOPZO010000142 DAOPZO010000000
VERSION     DAOPZO010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26105_length_4353_cov_8.488367"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..3066)
                     /locus_tag="PKI68_08155"
     CDS             complement(1..3066)
                     /locus_tag="PKI68_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX53703.1"
                     /translation="MTLSDLSIRRPVFAWMIMFGLIVFGSISLNRMGISQMPDIDFPV
                     LGVRVSWDGAAPEIMEAELVDKIEQLVISVDGLKEVRSSIRQGSATVELEFELNRNID
                     AALQEVQGALAELRLPLNVNRPVIQKNSTEGEPIMRLGISGTQTPREMVDYVETFLLD
                     QLQVIPGVGEVALSGFAERNLRLWVDTKKLQEYELTILDIQQAVEQEHSELAAGYIEN
                     PQQEMNLRIMGEGLTVEQVANIPITHRGGQPIQDATILLKEICRVEDGLSDIRRLVRI
                     SGEPGLSIGISKQRGANEVEVGRLVRAKMEEIQQSLPAGMKLQINVDLTRTIEQSVKA
                     TEHELVTAAILTALICFLFLGTWSSGFNVILSIPTSIVGTFTILYFSGFTLNTFTLLG
                     LALAIGIVVDDSIMVLENIVRHFEMGKDKVTAARDGSREIIFAAVATTAAVVAIFLPV
                     AFMSGVIGRFFYQFGVTMTAAVVLSLVEAVTLTPMRLSMMMVRHTKASWLERGSSAVF
                     RGLNRAYGWILPFVLKMRWPVLIGALLIFAYSLRLGTALRREFVPAQDLSYISVNLRA
                     PVGSSLALTDSRVKEVEAYLKSRPEILRYYTMIGSSSGAGTPNQAMMGLTLVERNQRQ
                     KTQAELMDEFRKDLARVKGLRISFQDTSARGLTARRSQPVEFNISGSDYAVLAASAQQ
                     IMDKLEKTGLVVDLDNSFRTGMPEVRIIPDRQAAAARGVTMEEIGRTINAAMGGIRQG
                     KFTRDGRRYDIRLRLSPEDRLTGDDVKTLTVRNSYGELIPLGDVVKIETASTLQNISR
                     INRQRAISLTANLATGASQAAALEAAKQISKETLPPGYTFNLEGGSKTFSESGSGLWI
                     AFLLGIVVAYMVLASQFNSFIHPFTVLLALPFSITGALVTLWGTNRSLNLYSAIGIIL
                     LMGVVKKNSILLVEFMNRKRAEGMPLREAIITAAPIRLRPILMTSVATIAAAFPIALG
                     LGVGSETRQPMALAIIGGVFVSTLFTLLVVPCAYLIMARFERTKHHPES"
     gene            complement(3063..4295)
                     /locus_tag="PKI68_08160"
     CDS             complement(3063..4295)
                     /locus_tag="PKI68_08160"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX53704.1"
                     /translation="MRKKQIFAVTGLCLLSITPMTLAMEVNLTAAYELAAAKSEVLQI
                     SAAEWRAAEARYRQAQGAMWPEVSARGDSRFIHAPDNDTRRAGVGATWTVFDGFRNAR
                     NADARQAEGIARSHDAEQARLLLYEDVADVFYQIRSFQDQREASREQLKTLETRADEL
                     NRRIKLGRSKMSDLLTTSNQMAEARLTIEQLNQSCAASLELLAFMTGLPAASLQLVDQ
                     TPLPAIGEVDNYLIAAADRADIKAGKAGVEASQFDMEAAKADRMPKVTLDGNSYLYRD
                     PQSQSEWDIVLSLEVPLFDKGRRKARIAEAGQQLRISELRLAELQRSSERDVRQAYES
                     LFYELKQWSELQSALGISSDTLTLLQKDYELGRVSNLDVLTASVQYWGLRRREAALAN
                     QLRADMIHLHVAAGKVSP"
BASE COUNT          869 a         1265 c         1304 g          915 t
ORIGIN      
        1 tcagctttca gggtggtgtt tggttctttc gaaccgggcc atgatgagat aagcgcaggg
       61 aacgacgagc agggtgaaca gggttgatac gaaaactccg ccgatgatgg ccagcgccat
      121 cggctgacgg gtttccgaac cgacgccgag tcccagtgcg atgggaaacg ctgcggcaat
      181 ggtcgcgacg gaggtcatca gaatcggccg cagccggatc ggggcggcgg tgatgattgc
      241 ttcacgcagc ggcatgcctt cggcgcgttt ccggttcata aattcgacca gcaggatgga
      301 gtttttcttc acgactccca tcagcaggat gatgccgatg gcgctataga gattcagcga
      361 ccggttggtg ccccacagcg tgaccagtgc accggtgata ctgaacggca gcgccagcag
      421 gacggtgaag gggtggatga agctgttgaa ctgcgacgcc agcaccatgt aggcgaccac
      481 aatccccagc agaaaggcga tccagagtcc gctgccggat tcgctgaacg ttttcgaacc
      541 gccttccaga ttaaaggtat agccgggcgg cagcgtttct tttgaaattt gtttcgccgc
      601 ttccagcgcg gcggcctgcg aggcgccggt cgccagattg gcggtcagtg aaatggcgcg
      661 ctggcggttg atgcggctga tgttctgcag cgttgatgcc gtttcaatct tcaccacgtc
      721 gcccagcgga atgagttcgc cgtaggagtt gcggacggtg agggttttga catcgtcgcc
      781 ggtcagccgg tcttcgggag aaaggcgcag ccggatgtca tagcgccgtc cgtcgcgggt
      841 gaattttccc tgacggatgc cgcccatggc ggcgttgatc gtgcgtccga tctcctccat
      901 ggtaacgccg cgcgcggccg cggcctggcg gtcggggatg atccggactt ccggcatacc
      961 ggtccggaag ctgttatcca gatcgacgac cagtccggtt ttttcgagct tatccatgat
     1021 ttgctgggcc gaagcggcca gtacggcata gtcggaaccg ctgatgttga attcgaccgg
     1081 ctggctgcgc cgggcggtca gtccgcgggc cgacgtgtcc tgaaatgaga tgcgcagacc
     1141 tttgacgcgt gccagatcct tgcggaactc atccatcagt tcagcctggg ttttctgccg
     1201 ctgattccgt tcgaccagcg tgagtcccat catcgcctga ttcggtgttc cggcgccgct
     1261 gctgctgccg atcatggtgt agtagcgcag aatttcggga cggctcttga gataggcctc
     1321 gacttccttc acccgcgaat cggtcagcgc cagcgatgag ccgacgggag cccggaggtt
     1381 gacggatatg tatgacaggt cctgcgccgg cacaaactcg cggcgcagtg ccgttcccag
     1441 ccgcagcgaa taggcaaaga tgagcagcgc tccaatgagg acgggccagc gcatttttaa
     1501 aacaaagggc aggatccagc cgtaggcccg gttcaatccg cggaacaccg cgctggaacc
     1561 gcgctccagc cagctggctt ttgtatgccg caccatcatc atggagagac gcatcggggt
     1621 gagcgtgacg gcttcgacca gcgacaagac cacggcggcg gtcatggtga cgccgaactg
     1681 gtagaagaag cggccgatta cgccggacat gaacgcgacc ggcaggaaga tcgcaacaac
     1741 ggccgcggtg gtggcgacgg cggcaaagat gatttcgcgc gatccgtcgc gggccgccgt
     1801 gactttgtct ttgcccattt caaaatgccg gacaatgttt tccagtacca tgatcgagtc
     1861 gtccaccaca atcccgatgg ccagcgccag gccgagcagc gtgaaggtgt tgagcgtgaa
     1921 gcccgaaaaa taaaggatgg tgaaggtgcc gacgatggac gtcgggatgg agaggatgac
     1981 gttgaagccg gagctccagg tgccgagaaa cagaaagcaa atcagcgccg tcagaatggc
     2041 cgccgtgacc agttcgtgtt cggtcgcttt gacggactgc tcgatggttc gggtcaggtc
     2101 cacgttaatc tgcaacttca tgccggccgg cagagattgc tgaatctcct ccattttcgc
     2161 tcggaccaga cggccgactt ccacttcgtt ggcgccgcgc tgtttgctga taccgatgga
     2221 cagccccggc tcgccggaaa tgcgcaccag ccgccggata tcgctcaggc cgtcttctac
     2281 ccggcagatc tctttcagga gaatcgtggc atcctgaatc ggctgtccgc cccggtgcgt
     2341 aatcggaatg ttggcgacct gttcaaccgt gagcccttcg cccataatcc gcaggttcat
     2401 ctcctgctgc gggttttcga tgtatccggc cgccagttcg gaatgctcct gctccacagc
     2461 ctgttgaatg tcgagaatgg tcagttcata ttcctgaagt ttttttgtgt ccacccagag
     2521 tctcaggttg cgttcagcga atccggacag tgcaacttct ccgacaccgg gaatgacctg
     2581 cagctggtcc agcaggaacg tttcgacata atccaccatc tcgcgcggtg tctgtgttcc
     2641 ggagatgccg agcctcatga tcggctcccc ctcggttgaa ttcttctgaa tcaccggtcg
     2701 gttcacattc agcggcagcc gcagttcggc taatgcgccc tgaacttcct gcaaggcggc
     2761 gtcgatgttg cggttgagct caaactccag ctcgaccgtg gccgacccct gccggatgct
     2821 ggaccggact tctttcaggc cgtctaccga gattaccagc tgctcgattt tatcaaccag
     2881 ctccgcctcc atgatctccg gcgcggcacc gtcccaggat acgcgaacgc cgagaaccgg
     2941 aaaatcgatg tcgggcatct ggctgatgcc catgcggttc aggctgatgg atccgaatac
     3001 gatcaggccg aacatgatca tccaggcgaa gaccgggcgg cggatggaaa gatcggataa
     3061 tgtcatggtg aaacctttcc ggcggcgaca tgaagatgaa tcatgtcggc gcgcagctga
     3121 ttggccagag ctgcttcccg ccggcgcaag ccccagtact gaacggaggc cgtgaggaca
     3181 tcgaggttgc tgacgcggcc cagctcatag tccttctgga gaagcgtgag cgtgtcggat
     3241 gagattccaa gagccgattg cagttccgac cattgcttca gttcgtagaa caaagattcg
     3301 taagcctgcc ggacatcccg ttcggaagag cgctgcaact cggccagtcg caattcgctg
     3361 attctcagct gctgcccggc ttcggcaata cgcgctttgc gccggccttt gtcgaacaga
     3421 ggaacttcca gactcagaac aatatcccat tcgctttgcg actgaggatc gcggtagaga
     3481 taactgttgc catccagcgt caccttaggc atccggtccg ctttggcggc ttccatatcg
     3541 aattgcgaag cctctacgcc ggctttaccc gctttgatgt ccgcccggtc tgccgccgcg
     3601 ataagatagt tatccacctc gccgatggcg ggcaggggcg tctgatcaac cagctggaga
     3661 ctcgctgccg gcaggcctgt cataaaggcc agcagttcca gggatgcggc acaggactgg
     3721 ttgagctgtt caatcgtcaa acgcgcttcg gccatctggt ttgaagtcgt cagcaggtcg
     3781 gacatttttg accgtccgag tttgatccgg cggttcagct catcagcccg ggtttccaat
     3841 gtcttgagct gttcccgtga agcttctcgc tgatcctgaa acgaccggat ctgataaaaa
     3901 acgtccgcga catcttcata gagaagcagg cgcgcctgtt ccgcatcatg ggagcgggcg
     3961 atgccttccg cctgtctggc atcggcattg cgcgcattcc ggaaaccgtc gaacaccgtc
     4021 caggtcgcgc cgactccggc ccggcgggtg tcgttatcgg gcgcatgaat aaatctggaa
     4081 tcgcctctgg cgctgacctc cggccacatg gcaccttgcg cctgccggta acgggcttcc
     4141 gccgcgcgcc attctgcggc actgatctga aggacttcgc ttttcgccgc agccaattcg
     4201 tatgccgccg tgagattgac ctccatcgcc agagtcatgg gagtgatcga caaaagacac
     4261 agccccgtga ctgcaaaaat ttgtttttta cgcatgagcc aattcatgca caacagcatc
     4321 ccatagtcag cagagagagc tcaattacca att
//
LOCUS       DAOPZO010000143         4342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_26202_length_4342_cov_8.477490, whole genome shotgun sequence.
ACCESSION   DAOPZO010000143 DAOPZO010000000
VERSION     DAOPZO010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26202_length_4342_cov_8.477490"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PKI68_08165"
     CDS             <1..377
                     /locus_tag="PKI68_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53705.1"
                     /translation="LAGYSVDRSRIYIDRHLQKTYQDKQGHHHEVDKFLILHEATEKA
                     MIDTWNLHYQHAHQIALRAEEAAVRAAGLEWREYDHFMQQWIKQADSEKITRVPANLD
                     LTPYLDENDNELIKVMQRAMVP"
     gene            480..1673
                     /locus_tag="PKI68_08170"
     CDS             480..1673
                     /locus_tag="PKI68_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX53706.1"
                     /translation="MNPITNPFAPGAGTPPPELAGRDELLKTIHIAIERTRLGFPTKS
                     VLLVGLRGVGKTVLLDRMRDDAETSGIQTLRIEAPENRSLPALLAPQLRQCLLRLSRN
                     EKAKELAQRALRGLAGFAKTLKVKYEDIEVGFDFDPEPGLADNGDLEHDLQALLEVVG
                     AAAKSAGTALVMFVDELQYVQETELEALIMALHRVSQRQLPVMLVGAGLPQLRGRMGR
                     AKSYAERLFDFPEIGPLNPDAAKQAILKPAADQNVIVEKDALERIVQETQGYPYFIQE
                     WGKHAWDAAEKSPITLQDVTLVSKGAIAALDESFFRVRFDRLTPAEKRYLRAMAELGA
                     GPHRSGDIADILGRKVSALGPTRGQLINKGMIWSPNHGDTAFTVPLFDGFMHRVMPGD
                     DWKKD"
     gene            1798..>4342
                     /gene="leuS"
                     /locus_tag="PKI68_08175"
     CDS             1798..>4342
                     /gene="leuS"
                     /locus_tag="PKI68_08175"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNX53707.1"
                     /translation="MNEKYPFSEIEPKWQAHWNEHGLFKTDLSKTAQKYYCLMMFPYP
                     SGKLHVGHGRNYIIGDAVVRYKKMRGFNVLSPMGWDAFGLPAENAAIKTGTPPRESTF
                     KNIAVMKEQLAAWGCCYDWEKEVTSCEPEYYKWTQWLFIQMFNRGLAYKKKSNVNWCP
                     NDQTVLANEQVIDGCCDRCGTVVVQKALEQWFFKITDYAQRLLDDIDKLEGWPERVKT
                     MQRNWIGRSEGSEIDFALVPRADGKADSSNKVSCFTTRVDTIYGCTYMVLAPEYANLK
                     EMIAGLPQEQEVLKFVADSAKLTNLDRESDALEKKGVFTGRYVVNPFTGEQVPLWVGD
                     YVLMYGTGAVMAVPAHDTRDWAFAKKYNLPVKLSIQNPEGSLVLAEMKNAYTEDGVCC
                     NSGEFTGLNNREAIRAMIQHAEAKGFGKGTINFRLRDWLISRQRYWGAPIPIVYCDRC
                     GMVPVNDDELPVLLPTDVEFKPTGESPLKGSASFMNCSCPKCGEPARRESDTMDTFVD
                     SSWYFLRYLNARDGSQAIDTEAANNWLPVDQYIGGIEHAILHLLYARFFTKVIHDLGL
                     IGFDEPFAKLFTQGMICKKSDIDGKLYKMSKSKGNVVSPNELIVNYGADTVRLYTLFI
                     GPPEKEAEWNDAAVEGSYRFLARIWRRVWQHRDILRGAAGPDAQAGKPAPLDMAKMEK
                     PERDLYRKLNESVKKITSDLDGAFHFHTAISTIMELMNAIDEIKVDDSSSEQVKAVFR
                     DAIEKVIVLISPFAPHISEELWKELGHTGGILAAKWPEVVEAALHRDEIQMALQVNGK
                     VRGQITVPSTATKEEIEKLAVTDEAVLRWSEGKAIKKVIVVPGRLVNIAV"
BASE COUNT         1052 a         1299 c         1176 g          815 t
ORIGIN      
        1 acctcgccgg ctacagcgtt gaccgctcca gaatctacat cgaccgccac ctgcagaaaa
       61 cctatcagga caaacagggc catcaccacg aagtcgacaa attcctgatc ctccacgaag
      121 ccaccgaaaa agcgatgatc gacacctgga acctgcatta ccagcacgcc caccagatcg
      181 ccctgcgcgc cgaagaagcg gcggtccgcg ccgccggact cgaatggagg gaatacgacc
      241 acttcatgca gcagtggatt aagcaggccg actccgaaaa aatcacccgc gttcccgcca
      301 acctcgacct cacgccctac ctcgacgaaa acgacaacga actcatcaaa gtcatgcagc
      361 gcgctatggt gccgtgataa agaaagtttc gccgactggg agaaaaccgg atatataggc
      421 aagttatttt aattattaaa aaattgtaat ttgactaatt tacctatata cgcctcttta
      481 tgaatccaat cacaaatcct ttcgcaccgg gtgcaggaac tcctcctcca gaactggcag
      541 gacgcgacga actcctaaaa accattcata tcgccattga acgaactcgg cttggctttc
      601 ctaccaagag cgttctgctg gtcgggttgc gcggcgtcgg caaaacagta ctgttagata
      661 gaatgcgtga tgatgcagaa acctctggaa ttcagacctt acgaatcgaa gctccggaaa
      721 accgctcttt gcccgccctg ctggctccgc aactccgtca atgcctcctt cgtctttccc
      781 gaaacgaaaa agcaaaagag ttggctcaac gggcgctccg cggacttgcc ggttttgcca
      841 aaaccctgaa ggttaaatac gaagacattg aagtggggtt tgattttgat cccgaaccgg
      901 gactagccga taacggagac ttggaacacg acctgcaagc cttattggaa gtagttggag
      961 ctgcagcaaa aagtgccggt actgcgctcg tcatgtttgt tgacgagctc caatatgttc
     1021 aggaaacaga actcgaagcg ctaattatgg cacttcacag ggtttcacaa cgtcagctgc
     1081 cagtcatgct ggtcggagcc ggcttgccgc agttacgtgg tcgtatggga agagcaaagt
     1141 cctacgcgga gcgtttattc gatttcccgg aaatcggccc gcttaatccg gatgccgcaa
     1201 aacaggccat cctgaaacct gccgcagatc agaatgtgat tgtagaaaaa gatgcgctgg
     1261 agcgaattgt ccaggaaacc caaggctacc cctacttcat tcaggaatgg gggaaacatg
     1321 cctgggatgc cgctgaaaaa tcaccgatta ctcttcagga cgtaacgctg gtttcaaagg
     1381 gagccattgc cgctcttgat gaaagtttct tccgcgtccg gtttgaccgt ctgacgccag
     1441 cagaaaaacg ctatctgcga gccatggccg aactcggagc tgggccacac cgttccggcg
     1501 atattgcgga catccttggc cgcaaggtga gcgctcttgg gccaacgcgc ggccagctga
     1561 tcaacaaagg aatgatctgg agtccgaatc acggagacac ggccttcacc gttcctttgt
     1621 tcgatggctt tatgcatcga gttatgccgg gcgacgactg gaagaaggac tgatctcacc
     1681 agcctcgatt cgacagctgg aaaccgcgtc cgcctttccg accaaaattg actcttccaa
     1741 tgcttggaaa atccgccgga atccgtagtc tgttgcggtt tacgcgagga atgtcctatg
     1801 aatgagaaat accctttttc tgaaattgaa ccgaagtggc aggcgcactg gaacgagcac
     1861 ggtctgttca agaccgatct gtcgaagacg gcgcagaagt attactgcct gatgatgttc
     1921 ccctatccgt ccggcaagct gcacgtcggc cacggccgca actacatcat cggcgacgcc
     1981 gtcgtccgct acaaaaagat gcgcggcttc aatgttctct cgccgatggg ctgggacgcg
     2041 ttcgggctgc ccgccgaaaa cgccgcgatc aagaccggta cgccgccgcg cgaaagcacg
     2101 ttcaagaata tcgccgtgat gaaggaacag ctcgccgcct ggggctgctg ctacgactgg
     2161 gaaaaggaag tcacttcctg cgagccggaa tattacaagt ggacgcagtg gctgttcatc
     2221 cagatgttca accgcgggct ggcctataag aagaagagca acgtcaactg gtgcccgaac
     2281 gaccagaccg tgctggcgaa cgagcaggtt atcgacggat gctgcgaccg gtgcggaacg
     2341 gtcgtggttc agaaggcgct cgaacagtgg tttttcaaaa tcaccgatta cgcccagcgg
     2401 ctgctcgacg atatcgataa gctcgaaggc tggccggagc gcgtcaagac gatgcagcgc
     2461 aactggatcg gccgctccga aggttcggaa attgatttcg cgctcgttcc gcgcgccgac
     2521 ggcaaagccg acagctcgaa caaagtctcc tgcttcacca cccgcgtgga caccatttac
     2581 ggctgcacct acatggtgct ggcgccggaa tacgccaatc tcaaagagat gatcgccggc
     2641 ctgccgcagg aacaggaagt gctgaagttt gtcgccgaca gcgcgaagct gaccaacctc
     2701 gaccgcgagt ccgatgcgct cgagaagaaa ggcgttttca ccggccgcta tgtcgtcaat
     2761 ccgttcaccg gcgaacaggt tccgctgtgg gtcggcgact atgtgctgat gtacggcacc
     2821 ggcgcggtga tggccgtccc ggcgcacgac acgcgcgact gggctttcgc gaagaagtac
     2881 aacctgccgg tcaaactttc gatccagaat ccggaaggct cgctggttct cgccgagatg
     2941 aagaacgcct acaccgaaga cggcgtctgc tgcaactccg gcgaattcac cggcctgaat
     3001 aaccgcgagg ccatccgcgc aatgattcag cacgccgagg ccaaaggctt cggcaaaggc
     3061 accatcaact tccgcctgcg cgactggctg atttcccgcc agcgctactg gggcgcgccg
     3121 attccgatcg tctattgcga ccgctgcggc atggttccgg tcaacgacga cgaactgccg
     3181 gtgctgctgc cgaccgacgt cgagttcaag ccgaccggcg agtcgccgct caaaggctcc
     3241 gcctcgttca tgaactgttc ctgcccgaag tgcggcgaac cggcgcggcg cgagtccgac
     3301 acgatggaca cgttcgtcga ctccagctgg tacttcctgc gctacctcaa cgcccgcgac
     3361 ggctcgcagg cgatcgacac cgaagccgcc aacaactggc tgccggtcga ccagtatatc
     3421 ggcggcatcg aacacgccat tctgcacctg ctctacgccc gcttcttcac caaggtcatc
     3481 cacgacctcg gcctgatcgg cttcgacgaa ccgttcgcca aactcttcac gcaaggcatg
     3541 atctgcaaga agagcgacat cgacggcaag ctctacaaaa tgagcaaatc caaaggcaac
     3601 gtcgtcagcc cgaacgagct gatcgtcaac tacggcgccg acaccgtacg cctctacacc
     3661 ctcttcatcg gcccgccgga aaaagaagcc gagtggaacg acgccgcggt cgaaggttcc
     3721 taccgcttcc tcgcccgcat ctggcgccgc gtctggcagc accgcgatat tctccgcggg
     3781 gcagccggcc cggatgcaca ggctggaaag cctgcgccac tggacatggc gaaaatggaa
     3841 aaaccggagc gcgaccttta ccgcaagctg aatgagtcgg tgaagaaaat caccagcgac
     3901 ctcgacggtg cgttccattt ccataccgcc atttcgacca tcatggaact gatgaacgcc
     3961 atcgacgaaa tcaaggtcga cgacagctcg tccgagcagg tcaaagccgt cttccgcgac
     4021 gccatcgaaa aagtgatcgt actaatttcg cccttcgccc cgcatatttc cgaagaactc
     4081 tggaaagagc tcggccatac cggaggaatc cttgcggcca agtggccgga agtggttgaa
     4141 gccgcgctgc accgcgacga aattcagatg gccctgcagg tgaacgggaa agtccgcgga
     4201 cagatcaccg tgccctccac ggcgacgaag gaagagatcg aaaaactcgc cgtcaccgac
     4261 gaagcggtcc tccgctggtc cgaaggcaag gcgatcaaaa aagtgatcgt cgtccccgga
     4321 cgtctggtga acattgcggt gg
//
LOCUS       DAOPZO010000144         4328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_26356_length_4328_cov_8.942897, whole genome shotgun sequence.
ACCESSION   DAOPZO010000144 DAOPZO010000000
VERSION     DAOPZO010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26356_length_4328_cov_8.942897"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PKI68_08180"
     CDS             <1..522
                     /locus_tag="PKI68_08180"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0044210 - 'de novo' CTP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine monophosphate kinase"
                     /protein_id="HNX53708.1"
                     /translation="GGTDRTTGDSMGMLATVINALALQSSLENVGIETRVQTAISMPA
                     VAEPFIRRKAMRHLEKGRVVIFGAGTGNPYFTTDSAAALRASEIGADVLMKATKVDGI
                     YTADPMKDKTATRFDKISYSDALHKRLKIMDAAAFSLCMENDIPIVVFNFFKEGEMLR
                     VFRGEDAGTLVGH"
     gene            539..1102
                     /gene="frr"
                     /locus_tag="PKI68_08185"
     CDS             539..1102
                     /gene="frr"
                     /locus_tag="PKI68_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNX53709.1"
                     /translation="MESSIEVLLAAEEKMDKCVEFLHHDLSGLRTGKASPSLVENITV
                     EYYGTATRLRDIANISTPEPRLLVISPFDPSSLGAIEKAISAANIGITPINDGRLIRV
                     PIPEMSEQRRKEMAKVAARATEDQRIAVRNVRRDANESLKALEKSGKITEDDRDQSLE
                     EVQKLTDNRIKKMDDMLAAKEKEMMVV"
     gene            1184..1735
                     /locus_tag="PKI68_08190"
     CDS             1184..1735
                     /locus_tag="PKI68_08190"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HNX53710.1"
                     /translation="MKRILIGVSGGIAAYKAAGVVSALKQAGHDVQVAMTPAACQFVT
                     PLTFAALSQKEVRTELFPAHPTSDKGQLYPHLYPATEADLFAVLPATADIIAKLAYGF
                     GDDIVSASALSLGKKCVKIFCPAMNTQMWDNPVVRENAQILENRGWQRIGPETGLMAC
                     GTEGAGRMSEPAAILEAINKALA"
     gene            1732..2400
                     /locus_tag="PKI68_08195"
     CDS             1732..2400
                     /locus_tag="PKI68_08195"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HNX53711.1"
                     /translation="MKKVLILSGPTHEYFDPVRFIGNASSGKMGKALAEEALKHGMEV
                     EFISGPVPDANLPGRDRSPSGPVGALGEHALPKLNITRVTSAEEMLAAAQAKFAAADL
                     ILFAAAVADYRPVKKLDEKLPKIGGNLTLKLQPTPDIAATLCANKRKDQIAIGFALQT
                     HDGEAKAREKLISKNLDGIVLNTPATLGAESGIFTWIDRQGLDNWGSIGKSACAKQIF
                     NQIL"
     gene            complement(2490..2993)
                     /locus_tag="PKI68_08200"
     CDS             complement(2490..2993)
                     /locus_tag="PKI68_08200"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNX53712.1"
                     /translation="MKKHIQAFLSEKENPFVQFIKYGMCGGAATAVDMTVFFLFAWLV
                     FPALTESDPFTKLLGFLNFDIRTVSETVRLRNYWIDKAICFLFSNFTAYVLNVLFVFK
                     AGKHKRHHELMLFYAVSLVSFFIGTFIGAWLIQGFGLDTTYSYVAAMISALLINYAGR
                     KFFIFHG"
     gene            complement(3062..3766)
                     /gene="lipB"
                     /locus_tag="PKI68_08205"
     CDS             complement(3062..3766)
                     /gene="lipB"
                     /locus_tag="PKI68_08205"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNX53713.1"
                     /translation="MNAWALQFSEPVPYQTGLDLQHRLLAARQRNEIPDTVLILQHTP
                     TVTLGNRGRDNYLLKTPEEYKALGIDVFHAERGGDVTYHAPGQWVLYPIIYLGGSEAD
                     AHGYLWNLEEVALRTLADFGIAGFRREGKNGAWTEQGKVAAIGFRLKRWVSFHGMSFN
                     VSLDLSGFQTIVPCGLVGQPVASMKTVLGESCPSMEAVRDRLLANFSAVCGRDLETFR
                     TLEESPAKLRKIISEA"
     gene            complement(3802..4203)
                     /gene="gcvH"
                     /locus_tag="PKI68_08210"
     CDS             complement(3802..4203)
                     /gene="gcvH"
                     /locus_tag="PKI68_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNX53714.1"
                     /translation="MSVPNDLYYAKTHEWLRIDDNMATVGITDFAQNQLSDITFVELP
                     DVGREVEAGDEVAVVESVKAASDIYAPVAGTIVEVNVNLEDSPDMINASPYDAGWLFK
                     IEVRKEEDVDNLMSAEEYAESCDEEPDVEED"
     gene            complement(4241..4314)
                     /locus_tag="PKI68_08215"
     tRNA            complement(4241..4314)
                     /locus_tag="PKI68_08215"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4280..4282),aa:Cys,seq:gca)
BASE COUNT         1037 a         1259 c         1194 g          838 t
ORIGIN      
        1 ggcggaaccg accgcaccac cggcgactcc atgggcatgc tcgccaccgt catcaacgct
       61 ctggctctgc aatcctcgct cgaaaatgtc ggcattgaaa cgcgcgttca aacggccatc
      121 agtatgcccg ccgtcgccga accgtttatc cgccgcaaag ccatgcgcca ccttgaaaaa
      181 ggccgcgtcg tcattttcgg cgcaggcacc ggcaacccct acttcacgac cgacagcgcc
      241 gccgcactgc gcgcttctga aatcggcgcc gacgtgctga tgaaggccac caaggtggac
      301 ggcatttata ccgccgaccc gatgaaggat aaaaccgcca cccgttttga caaaatttcc
      361 tacagcgacg cgctccataa gcggctcaaa atcatggatg ccgccgcgtt ctcgctctgc
      421 atggaaaacg atattccgat cgttgtgttc aacttcttca aagaaggcga aatgctgcgg
      481 gttttccgcg gtgaagatgc cggaacgctg gtcgggcact gaaatttaag gagacgttat
      541 ggaatcaagc atagaagtac tgctggccgc cgaagagaag atggataaat gcgttgagtt
      601 cctccatcac gacctcagcg gactgcgcac cggcaaagca agcccaagcc tggtggaaaa
      661 catcaccgtc gaatattacg gcaccgccac ccgcctgcgc gatatcgcca acatttcaac
      721 tcccgaaccg cgcctgctgg ttatcagccc gttcgacccc tcttcgctcg gcgccatcga
      781 aaaagccatc agcgccgcca acatcggcat tacaccgatc aacgatggcc gactcatccg
      841 cgtgccgatt ccggaaatga gcgaacagcg ccgcaaagaa atggctaaag ttgccgcacg
      901 cgccacggaa gatcagcgca tcgctgtccg caacgtccgc cgcgacgcca acgaaagtct
      961 caaagccctc gaaaaaagcg gaaaaatcac cgaagacgac cgcgaccaga gtctggaaga
     1021 agtccagaag ctgaccgaca accggattaa gaaaatggac gacatgctcg ccgccaaaga
     1081 aaaagaaatg atggtggtgt aagggaaagg atgaagtatg agatgtgaaa gatgaaaaag
     1141 gcatgttcca ttttcatagt tcatcattca tagttcacag ttcatgaaac gaatcctcat
     1201 aggtgtaagc ggcggaatcg cggcatacaa ggccgccggc gtcgtcagcg cgctcaaaca
     1261 ggccggacat gacgtacagg tggccatgac accggccgcc tgccagttcg ttacgccgct
     1321 cacctttgcc gcactgtcgc agaaagaagt acgcacggaa cttttcccgg cgcacccgac
     1381 ctccgacaaa ggtcagctct acccgcacct ctaccccgct accgaggccg atctcttcgc
     1441 cgtcctgccc gccacggctg acatcatcgc caaactggcg tacggcttcg gcgacgacat
     1501 cgtttccgcc agcgcgctgt cgctcggcaa aaagtgcgtc aaaattttct gcccggccat
     1561 gaacacgcag atgtgggaca accccgttgt ccgtgaaaat gcgcagattc ttgaaaaccg
     1621 cggctggcag cgcatcggcc ccgaaaccgg cctcatggcc tgcggcaccg aaggcgccgg
     1681 gcggatgagc gaaccggccg ccattctcga agcgattaac aaagccctcg catgaaaaag
     1741 gtcctcatcc tttccgggcc gacacacgaa tactttgatc cggtccgctt tattggcaac
     1801 gccagctccg gcaaaatggg caaagccctc gccgaagaag cgcttaaaca cggcatggaa
     1861 gtcgaattta tctccgggcc ggtgccggat gcgaatcttc caggtaggga ccgctcgccg
     1921 agcggtccgg tcggcgcgct cggcgagcac gccctaccga aattgaatat cacgcgagtc
     1981 acctccgccg aagaaatgct ggccgccgcg caggcaaaat tcgccgccgc cgacctgatc
     2041 ctcttcgccg ccgccgtcgc cgactaccgg cccgtgaaaa agctggacga aaaactccca
     2101 aagattggcg gtaacctcac gcttaagctg caaccgacgc ctgacatcgc cgcgacgctc
     2161 tgcgcgaata agcggaaaga tcagatcgcc atcggctttg cgctccagac gcacgacggc
     2221 gaagccaagg cgcgcgaaaa actgatttca aaaaatctgg acgggatcgt cctgaacacg
     2281 cccgccaccc tcggcgccga atccggcatc ttcacctgga tcgacaggca gggtttggat
     2341 aactggggtt cgatcggcaa gtccgcctgc gcgaagcaga tcttcaatca gatattataa
     2401 attccctcgc agagattctg agcgcgcaga gagaaattaa tggagattta ttctttgcgg
     2461 tctttgcgcc tctgcgaggt gtcatccccc tacccatgaa aaatgaaaaa cttgcggccg
     2521 gcgtagttga tgaggagcgc ggagatcatg gcggcaacat aggaataggt tgtgtcgagt
     2581 ccgaagcctt ggatgagcca tgcgccgatg aaggtgccga tgaagaagga aaccagcgag
     2641 acggcgtaga agagcatgag ttcatggtgc cgtttatgct tgccggcttt gaatacgaac
     2701 agaacgttga gaacgtaagc ggtaaagttg gagaagagaa agcagatggc tttatcgatc
     2761 cagtagttcc gcaggcggac ggtttcggag acggtgcgga tatcaaagtt cagaaagccg
     2821 agcagtttgg tgaacgggtc gctttcggta agcgccggga agaccagcca ggcaaacaga
     2881 aagaaaacag tcatatcgac agcggtggcg gcgccgccgc acattccgta tttgatgaac
     2941 tgaacgaacg ggttttcttt ttccgagagg aacgcctgaa tgtgtttctt cataggcgcg
     3001 aagtctgcct gcaagccgtt aaagaatcaa accgcgaaat cccgaaatcg aaaaagtacg
     3061 gtcaagcttc agagatgatt ttccgcagtt ttgcagggga ttcttccagc gtccggaagg
     3121 tttcaaggtc gcggccgcag actgcggaaa agttggcgag aagccggtcg cggacggctt
     3181 ccattgacgg acaggattca ccgagaacgg tcttcattga ggcgaccggc tggccgacca
     3241 gtccgcacgg tacaatggtc tgaaagccgc tcagatcgag gctgacgtta aagctcatgc
     3301 cgtgaaacga gacccaccgt ttaaggcgga acccgatggc ggcgactttg ccctgttccg
     3361 tccacgcgcc gtttttccct tcgcgccgga atccggcgat accgaagtcg gcgagcgtgc
     3421 gcaaggcgac ttcttccagg ttccagagat agccgtgcgc gtcggcttca gagccgccga
     3481 ggtagatgat cgggtaaagc acccactgcc ccggtgcgtg ataggtgacg tcgccgccgc
     3541 gttcggcatg gaacacgtcg atgcccaacg ctttgtattc ttccggcgtt ttgagcagat
     3601 agttatcgcg gccgcggttg ccgagggtga cagtcggcgt gtgctgaaga atcaggaccg
     3661 tatcgggaat ttcgttgcgc tggcgcgcgg cgagcaggcg gtgctgcaga tcgagcccgg
     3721 tctgatacgg aaccggttca ctaaactgca gggcccaggc attcatacaa taaaaaaggg
     3781 cggccgggcc gccctttcgt tttagtcttc ctcgacatcg ggctcttcgt cgcaactctc
     3841 ggcatactct tcagcgctca tgaggttgtc gacatcttct tctttgcgaa cttcgatttt
     3901 gaagagccag ccggcatcgt acgggctggc gttgatcatg tccggagaat cttcaaggtt
     3961 cacgttcact tccacgatcg ttccggcgac cggagcgtaa atgtcagagg cggctttcac
     4021 ggattccacg acagcgactt catcaccggc ttcaacttcg cgaccgacat cgggcagctc
     4081 aacaaaggtg atatcggaca gctgattctg ggcaaaatcc gtaataccga cagttgccat
     4141 gttgtcatcg atccggagcc attcgtgggt tttcgcataa tacaggtcat tcggtactga
     4201 catttctctt ctccttcgct gatcgcttca attacaaatc tggaggcgcg aagcggaatt
     4261 gaaccgctct agcaggtttt gcagacccgt gcctaacctc tcggccatcg cgccctcgtt
     4321 aaaggctc
//
LOCUS       DAOPZO010000145         4184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_27719_length_4184_cov_10.352143, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000145 DAOPZO010000000
VERSION     DAOPZO010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27719_length_4184_cov_10.352143"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..81)
                     /locus_tag="PKI68_08220"
     tRNA            complement(5..81)
                     /locus_tag="PKI68_08220"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45..47),aa:Met,seq:cat)
     gene            153..923
                     /locus_tag="PKI68_08225"
     CDS             153..923
                     /locus_tag="PKI68_08225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNX53715.1"
                     /translation="MNLILIHPHEPDREGRVRLCGERAEHICTVLRAGSGKALRIGLL
                     NGPLGSGTVEEIRGKEVVLRCEFEGQIPARPAVDLLLAMPRPKVMKRLWAQLAALGVG
                     RIILVNAEKVERFYFDSHVLEPDFYAARLAEGLQQAGDTIVPEVRIVKELKPFLEDEL
                     DRVFPADGIRLLADPSGAQNIFQCLEKSNIPAPRGDVPQRSCLAVGPEGGWTPYELEM
                     FTAHGFEVFNAGTRILRTDTACVALLSMLTLLSGADEK"
     gene            913..1383
                     /locus_tag="PKI68_08230"
     CDS             913..1383
                     /locus_tag="PKI68_08230"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNX53716.1"
                     /translation="MKNKWVQYLATGLGFGFSPVLPGTCGTLWGLPLAWAVAQLPALT
                     FQIVACMALALLAVPVCSAAEKQLGKGKDPGCIVADEYCTLPMVYLGLPLTPGVLLSG
                     FILHRIFDIIKPPPIRQLQSLKGGIGIVIDDFVAALFALACNQLLFRVVLPALK"
     assembly_gap    1385..1484
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1485..1794
                     /locus_tag="PKI68_08235"
     CDS             <1485..1794
                     /locus_tag="PKI68_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53717.1"
                     /translation="GKMPITLDAGASAVKKAVSKPFIFTVKDVDVVNSNGVRAGLGLS
                     RQIRGGDEYAGYIVLVRAAGDILQKDSNSSRFLNDEWLAQCDKFSKITPAAKKKKKKK
                     "
     gene            complement(1804..2853)
                     /locus_tag="PKI68_08240"
     CDS             complement(1804..2853)
                     /locus_tag="PKI68_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HNX53718.1"
                     /translation="MKKILSVCLSVLVLNVSAEERIPKNIILFIGDGMGLSQVTAGKI
                     AKGKLEMERCPVTGLVTTWSASSLVTDSAAAATALSTGTKTRNGALGMADDKPLKNVM
                     EYAKEKNKSTGVAVVCALTHATPAGFIVHVPGRKMDTEIAQQIAGSDMDVLFGGGLDH
                     FNPTNNPSLPELQKKMTVALTADEFRKIGTPEKAAALLYPVHPPYAAERTVSLEEMTQ
                     KALEILSQDKDGFFLMVEGSQIDWAAHKNNGTNVVSEVVDFDNAVGAGLNFSAENGET
                     LVIITADHETGGFAVLGGSVKNKTVNKTGFVHGDHTASMVPLFASGPGSAVFSGIHDN
                     TDIGKTMIRYIKGIE"
     gene            complement(2915..3370)
                     /locus_tag="PKI68_08245"
     CDS             complement(2915..3370)
                     /locus_tag="PKI68_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53719.1"
                     /translation="MREYKWTAVGLIAAFTLWAVTVSLDLDLFETFASCLQQYESIEA
                     DEIIFPVVIFNIFLLIDLIRRNQSSRVEIEKCRVYRSTLKAMNHILNNFLQKMLLFKL
                     TAEETKGFDPEVLKRYDTIIDEAKAQIDALECIAKPDEKTILDTIGDPL"
     gene            3643..>4184
                     /locus_tag="PKI68_08250"
     CDS             3643..>4184
                     /locus_tag="PKI68_08250"
                     /inference="COORDINATES: protein motif:HMM:NF021882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNX53720.1"
                     /translation="MSTTDTIAAIATPPGQGGVGIVRVSGPKVWKIADAIFLSSEKPS
                     AAQGGTFLHGKVLAADGTEIDEALCLIFRAPKSYTGEDTVEIQGHGGPVVLKKILRRC
                     LEEGARMAEPGEFTKRAFLNGKMDLVQAEAVADLIHAQSDRAAKAALEQLEGGLSKQF
                     NRLYDGLMETAADIETTLDFV"
BASE COUNT          889 a         1073 c         1146 g          976 t
ORIGIN      
        1 aaggtggtgg ctggaggcgg acttgaaccg ccgacccgcg gattatgatt ccgctgctct
       61 gaccaactga gctacccagc cataagaaag ggttcagaac atagaaaaag ccgttcgggt
      121 tagcaagagc aatgtggtaa gaatccctgc gaatgaacct tattcttatc catccgcatg
      181 aaccggaccg cgaaggccgc gtacgccttt gcggcgagcg ggcggagcat atctgcaccg
      241 tgttgcgcgc cggttccggt aaagcgctgc gcatcggcct gctgaacggg ccgttgggaa
      301 gcggaacggt cgaggaaatt cgcggcaaag aagttgtttt acgctgtgaa tttgaaggac
      361 agattcctgc ccgtccggcc gttgacctgc ttcttgcgat gccgcggccc aaggtgatga
      421 aacgtttatg ggcgcagctg gccgcgcttg gtgtcggccg catcattctg gtcaacgcgg
      481 aaaaggtgga gcggttttac ttcgacagcc atgttcttga accggatttt tacgccgcgc
      541 ggttggccga agggctccag caggcgggcg atacgattgt gccggaagtc cggatcgtca
      601 aagaactcaa accgtttctg gaagacgagc tggacagggt gtttccagcg gacggaatcc
      661 gcctgcttgc cgatccgtcc ggtgcgcaga atatttttca gtgtttggaa aaaagcaata
      721 ttcctgcacc gcggggcgat gtgccgcaac gcagctgtct ggcggtcggg cccgagggag
      781 gctggacgcc gtacgaactg gaaatgttca cagcgcacgg atttgaagtt tttaatgccg
      841 gtacgcgcat tctgcgtacc gatacggcct gtgttgcatt gctttcaatg cttacgctac
      901 tctccggtgc agatgaaaaa taaatgggtg caatatttgg cgacaggttt gggattcggt
      961 ttcagcccgg ttctgcccgg cacatgcggt acgctgtggg ggcttccgct tgcgtgggcg
     1021 gttgctcagc ttccggcgct gacatttcaa atcgtggcgt gtatggcgct ggctctgctg
     1081 gcggttccgg tttgttcggc ggctgaaaaa cagcttggca aaggtaaaga tcccggctgt
     1141 atcgttgcgg atgaatactg cacgctgccg atggtttatc tcgggctgcc tcttacgccg
     1201 ggggtgcttt tgtccggctt cattcttcat cgtattttcg atatcatcaa gccgccgccg
     1261 atccggcagc tgcaaagtct gaagggcggt atcggaattg tgattgatga ttttgtcgcc
     1321 gcgcttttcg cactggcttg caaccagttg ctgttcagag tagttttgcc ggcgctaaaa
     1381 tagannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagggaa aatgccgatc
     1501 actctcgatg cgggcgcaag tgctgtaaaa aaagcggtgt ccaagccgtt tatctttacg
     1561 gtcaaagatg ttgatgtagt aaacagtaac ggagtcaggg ccggcctcgg cctttcacgg
     1621 cagatccgtg gcggagatga atatgccgga tatattgttt tggttcgcgc ggccggggat
     1681 atcctccaga aagacagcaa ctcgtcccgg tttctgaacg atgaatggct ggcgcagtgc
     1741 gataaatttt cgaaaataac gccggccgcc aagaaaaaga aaaagaaaaa gtaaacggcc
     1801 agcttattct atacccttga tataccggat cattgttttc ccgatatccg tattatcatg
     1861 gatgccgctg aacaccgcgc tgcccgggcc ggaggcaaac aacggaacca ttgaagccgt
     1921 gtgatcaccg tggacaaaac cggttttatt cacggttttg tttttgacag aaccgcccag
     1981 gacggcgaat ccgccggttt cgtggtcggc ggtgatgatc accagagttt ccccgttttc
     2041 cgcagaaaag ttgaggccgg cacctaccgc attatcgaaa tcaaccactt cgctgacgac
     2101 attcgtcccg ttgtttttat gggcggccca gtcgatctgc gatccttcta ccatcaggaa
     2161 gaatccgtct ttatcctgcg acagaatttc gagggccttt tgtgtcattt cttccaggga
     2221 cacggtgcgc tcggcggcat agggcggatg aaccggataa agcagggcgg cggctttttc
     2281 cggcgtgccg attttacgga attcgtccgc cgttaatgcg acggtcattt tcttttgcag
     2341 ctcgggcagg ctcggattgt tggtcgggtt gaaatgatcc agtcctccgc cgaagagaac
     2401 atccatgtcg ctgccggcaa tctgctgtgc aatttcggtg tccattttgc ggccgggcac
     2461 atgcacaata aatcctgccg gtgtggcgtg cgtcaatgcg cagacaacgg ctacgccggt
     2521 ggatttgttt ttttctttgg catactccat tacatttttc agcggtttat cgtccgccat
     2581 tcccagtgca ccgttgcggg tttttgtgcc ggtggaaagc gcggtggcgg ccgctgccga
     2641 atcagttacc agcgagctgg ctgaccaggt tgttacgagc ccggtcaccg ggcagcgttc
     2701 catctcaagt tttcctttgg cgatttttcc ggcagttact tgagacaggc ccatgccgtc
     2761 gccgatgaaa agaataatgt ttttaggaat acgctcttcg gcactaacgt ttagaaccaa
     2821 aaccgacaga cagactgaca gaattttttt cataaggcgg gcagtgtagt tccgtctgct
     2881 tttattaaac aagcccggaa tcaggccgtt tcgatcagag cggatcgccg attgtgtcga
     2941 gaatggtttt ctcatcgggc tttgcaatgc attcaagtgc gtcgatttgg gccttggcct
     3001 catcgatgat ggtgtcatac cgtttcagca cttcgggatc gaaccctttg gtttcttcag
     3061 cggtcagttt aaacagaagc attttctgga ggaaattatt cagaatatga ttcatggcct
     3121 tcagcgtaga ccggtaaacc ctgcactttt caatctccac ccggctgctc tggttccggc
     3181 ggatcagatc aatcagtaag aaaatattga aaatcacaac agggaaaatg atctcgtctg
     3241 cttctattga ttcgtactgc tgcagacagg atgcaaaggt ttcaaacaga tccagatcca
     3301 gactgaccgt taccgcccaa agggtgaacg ccgcaatcag cccgacagct gtccatttgt
     3361 attcccgcat aaaggctcct gatttaaagc cgtacacccg caggtgtctg cccagtttaa
     3421 ataaaaagac ggaaactgaa agacggccat acccctattc gtgtgtaatg agtttcctga
     3481 cggcattcag gcgcgctatt cccggtgcgc gaagctgaca gctgcagggg ccggccgttt
     3541 cccttatttc cgtcctgcgt cagagatttt catgggaacg tagcggcggc ggtctaccat
     3601 tttcttcgcc ttggcagccg ccttttgcta gaacggcagc cgatgagtac caccgacacg
     3661 atcgccgcta ttgcaacgcc gcccgggcag ggcggcgtcg gcattgtccg cgtcagcggc
     3721 ccgaaggtct ggaaaatcgc cgacgcgatc tttctgagtt cggagaagcc gtctgccgcg
     3781 caaggcggaa cctttctgca tggaaaagtc ctcgctgcgg acggcaccga aattgacgaa
     3841 gcgctctgtc tcatcttccg cgcgcccaaa agctacaccg gcgaagacac cgttgaaatc
     3901 cagggccacg gcggcccggt cgtcctgaaa aaaatcctgc gccgctgcct tgaagaaggc
     3961 gcgcgcatgg cagaacccgg cgaattcacc aaacgcgctt ttctgaacgg caaaatggat
     4021 ctggtccagg ctgaagccgt tgccgacctc atccatgcac agagcgaccg cgccgccaaa
     4081 gccgctctgg aacagctcga aggcggactc agcaaacagt tcaaccggct ttacgacgga
     4141 ctcatggaaa ccgccgccga cattgaaacc acgctcgact ttgt
//
LOCUS       DAOPZO010000146         4181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_27745_length_4181_cov_10.379787, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000146 DAOPZO010000000
VERSION     DAOPZO010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27745_length_4181_cov_10.379787"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..589
                     /locus_tag="PKI68_08255"
     CDS             74..589
                     /locus_tag="PKI68_08255"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNX53721.1"
                     /translation="MRKRSGKSVKFYLKLMRHPGTPESVGRGVAAGLFSAFIIPAGHM
                     PLAFALAMLVRGARGASLLSTWIVNPLTVSVIYPVQCYIGSFLIGDLLSYPEIKNLVL
                     DAMTNPSLKTAGTLGGELIVSFLVGGMLLGVLAAISGYFCTIVMVRRYRAQKARRKTA
                     RMDCRKTTEDC"
     gene            613..1281
                     /locus_tag="PKI68_08260"
     CDS             613..1281
                     /locus_tag="PKI68_08260"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HNX53722.1"
                     /translation="MREWLGAGVIALLLSLVWFWLAETVCPAAGKVLIGLTWLLWLAV
                     AAFFRVPNRRIIADANALVSPADGVVRDIETVEDHGIEIFAGQKLTRIGIFLSVLDVH
                     VNRAPCDLEVKYRKYKEGRFLDARDKRCSKENESLVIAGMGSAAGKTFPVAIRQVSGA
                     IARRIVCVPQPGDALKKGEIYGMIKFGSRTELYFPGEIGITVCVQIGDKVRSGSTLMG
                     RVEN"
     gene            1292..2119
                     /locus_tag="PKI68_08265"
     CDS             1292..2119
                     /locus_tag="PKI68_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HNX53723.1"
                     /translation="MINESIDEEDDEARFNPLPSLITLCNAFCGFASIVYAIASYARG
                     EALPEACIWLLLGAMVFDTLDGLAARMLHAKSLHGANLDSLADAISFGVAPAVTVFVF
                     IRRNCPVASTGGILMIWAVALFYLGCTLWRLARYNTLLAGGKKDDGCFTGLPSPAGAS
                     MIYSAGLFLPRLNLGPHLFFYFLLGYAFLTGLLMVSNVPYPHARRCVSGEPRVLSFVF
                     VCVVLGSIAVFGLTAMVIWAYIYFLIAPLAELLVRKRAGRPALALLFSALGRKPSAR"
     gene            2128..2817
                     /locus_tag="PKI68_08270"
     CDS             2128..2817
                     /locus_tag="PKI68_08270"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HNX53724.1"
                     /translation="MKTAAYSVGCGCLLLCFLSGTAAAQRISFRAIPPGWSVEGKPFT
                     KASIFEVQNNPAAPGHQWLSVTSEKASASLLSSYPLPVNLNKTPIIRWRWRATVLPPG
                     ADGRDPEKDDQVIGLYVSSGSRFRQQSIAYRWETQTPVGAEGTVRYASLFAVKWFTVR
                     NENHVDGETFFIEERNVAEDFKKTFGAVPDEIGIGISCNSQYTGSKSAAQLDWIEFCE
                     ADTKQTTLASD"
     gene            2832..3569
                     /locus_tag="PKI68_08275"
     CDS             2832..3569
                     /locus_tag="PKI68_08275"
                     /inference="COORDINATES: protein motif:HMM:NF041646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HNX53725.1"
                     /translation="MTNPRPKEENPMISILLNVVIPVVILTCLSKDKYLGPVWGLVVA
                     LILPIGYGIHTLIKERRADFVAIIGIISVLLTGVFGILKLPPQWIAVKEAAIPLIIGL
                     AIVISLKTPYPLIKKILMSEKLFNVPLLHEKLREQGNETTFEKRLVGLTWGLASSFFL
                     SSALNYTLAKIVLKSPPGTEAYAAEIGKMTGLSYIVVMLPSMAVMILVLMALIKTLTH
                     LTGLKLDDLLAEHLQEPKKETESVISE"
     gene            complement(3654..4025)
                     /locus_tag="PKI68_08280"
     CDS             complement(3654..4025)
                     /locus_tag="PKI68_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53726.1"
                     /translation="MSAQPEKLSDVKMDIANLYREESYTDLRTGGIRKLVPVKVDGSE
                     DTARSPFFSGHTQLMSPHGPLPLQGTIDAKTLEEAILKFPQAMEDAMNRMIEEAQQYQ
                     REQANKIITPADLKKDGGLII"
BASE COUNT          875 a         1148 c         1157 g         1001 t
ORIGIN      
        1 tcgcaaaatt cggagagaaa agttccaata ttcaaaaact ccgctatgct atcccctttt
       61 tgaaagagcc ttcatgcgga agcgatcagg caaatcagta aagttttatt tgaagctgat
      121 gcggcatccg ggcactcctg agagtgtcgg acgcggcgtc gctgccggac tgttttcagc
      181 cttcatcatt ccggccggac atatgccgct ggcctttgcg ctggccatgc tggttcgcgg
      241 cgcgcgcggc gcgtccttat tgtcgacctg gatcgtcaac ccgctgacgg tttcggtgat
      301 ttatcccgtt cagtgttata taggcagttt tttaatcgga gatctgttat cctatccgga
      361 aattaaaaac ctggttttgg atgccatgac caatccgtcg ctgaaaacgg caggaacgct
      421 gggcggtgaa ttgattgttt cctttcttgt cggcggaatg ctgctgggag tactggcagc
      481 gatatccggt tatttttgta caattgtaat ggtgcggcgc taccgggcgc agaaagccag
      541 acggaaaacg gcacgaatgg actgccggaa aaccacggag gattgctgat tatgcggctt
      601 acaaaacatg gaatgagaga atggctgggc gccggagtta ttgcgctgct gctgtcactg
      661 gtctggttct ggctggcaga aaccgtctgc ccggcagccg gaaaggtgct gatcggatta
      721 acctggctgc tgtggctggc cgttgcggct ttcttccggg ttccgaaccg ccggattatt
      781 gcggatgcca atgcgctggt ttcgccggcc gacggcgtgg tgcgtgacat cgaaaccgtg
      841 gaagaccacg gaattgagat ttttgccgga cagaaactga cgcgaatcgg tatttttctg
      901 tcggtgctgg acgtccatgt gaaccgtgcg ccgtgcgacc ttgaggtaaa gtaccgcaag
      961 tacaaagaag gccgctttct ggacgcccgg gacaaacgct gttcgaagga aaacgaatcg
     1021 ctggtgatcg ccggcatggg ttcagccgcc ggcaaaacct ttcccgtcgc aatccgtcag
     1081 gtttcgggcg caattgcccg ccggattgtc tgcgtccctc agccgggcga tgcattgaaa
     1141 aaaggcgaga tttacgggat gatcaagttt gggtcgcgta ccgagttgta tttccccggc
     1201 gaaatcggca taaccgtttg cgtacagatc ggcgataagg tccggtctgg cagcactctg
     1261 atgggtcgtg tcgaaaactg attcaacggc tatgataaac gaatctattg atgaagaaga
     1321 tgatgaagcc cggtttaatc ccctgccgag tctgatcaca ttgtgcaacg ccttttgcgg
     1381 ctttgcatcg attgtctatg cgattgccag ctatgcgcgc ggcgaagcgc ttccggaagc
     1441 ctgtatctgg ctgttactgg gcgcaatggt atttgatacc ctcgacgggc tggcagcgcg
     1501 aatgctgcac gcgaaaagtc tgcacggcgc caatctggat tctctggcgg acgcgatttc
     1561 tttcggcgtg gctccggcgg tcactgtttt tgttttcatc cggcgcaact gccccgtcgc
     1621 ctcgaccgga ggcatattga tgatctgggc tgtcgccctc ttttatctcg gctgcaccct
     1681 gtggcggctc gcgcgctaca acacgctgct cgccgggggt aaaaaagatg acggctgttt
     1741 tacgggcctt ccgtcgccgg ccggcgccag catgatttac agtgcgggac tttttcttcc
     1801 gcggctgaat ctcggcccgc acctgttttt ctacttcctg ctgggatatg cctttctcac
     1861 cggcctgctg atggtcagta acgttcctta cccgcatgcg cgccgctgcg tctcgggaga
     1921 accccgtgta ctcagcttcg tgtttgtctg cgtggttctt ggctccattg ccgttttcgg
     1981 cctgaccgca atggtgatct gggcttacat ctatttcctg atcgctccgc tggccgagct
     2041 gctggtgaga aaacgggccg gacgtccggc tctggctctc cttttttctg cactgggacg
     2101 aaaaccgtcg gcccggtaac tgcgaacatg aaaacggcgg catactctgt aggttgcgga
     2161 tgcctgctgc tctgtttcct gtccggcaca gccgccgccc agcgtatctc ttttagagcc
     2221 atacctccgg gctggtcggt ggaaggcaag ccgtttacca aagcctccat cttcgaagtt
     2281 cagaataatc cggcagctcc cgggcatcag tggctttcgg taacttctga aaaagccagc
     2341 gcatctctgc tgtcaagcta tcctctgccg gtgaatttaa ataaaacgcc gattatccgc
     2401 tggcgctggc gtgcaaccgt tcttccgccc ggagccgacg gccgcgaccc ggaaaaagac
     2461 gaccaggtca tcggccttta cgtcagctcc ggcagcagat tccggcagca gtcgatcgcc
     2521 taccgctggg aaacacagac gccggtcggc gcagaaggca ccgttcggta cgccagcctg
     2581 ttcgccgtca aatggttcac ggtccgcaac gaaaaccatg tggacggcga aacctttttc
     2641 attgaagaac ggaacgtcgc cgaggatttt aaaaaaacat tcggtgcggt gccggacgaa
     2701 atcggaatag gaatttcctg taacagccaa tatacgggct ccaaaagcgc ggctcagctg
     2761 gactggatcg aattctgcga agcggacacc aaacagacga ctctggcctc tgactgattc
     2821 gaaaggaacc catgaccaat ccccgtccta aagaagaaaa cccgatgatc agcatcctgc
     2881 tgaatgtcgt catccccgtc gtcatcctga cctgtttgag caaagacaaa tacctgggcc
     2941 cggtctgggg tctggtcgtc gcgctgattc tcccgatcgg ctacggcatc cacacgctga
     3001 tcaaagaacg gcgcgcggac tttgttgcga tcatcggcat catcagcgtc ctgctgaccg
     3061 gcgtgttcgg gattctgaaa ctgccgccgc agtggattgc cgtcaaagaa gccgccattc
     3121 cgctgattat cggactggcg attgtgattt cgctgaaaac gccttacccg ctcatcaaaa
     3181 aaatcctgat gagcgaaaaa ctgttcaacg tgccgctgct gcacgaaaaa ctgcgcgaac
     3241 agggtaacga aacgacgttc gaaaaacggc tggtcggcct gacctggggt ttggcttctt
     3301 cattttttct ctcgtcggca ctcaactaca cgctggcaaa aatcgtactg aaaagtccgc
     3361 cgggcaccga agcctacgcc gctgagatcg gcaaaatgac cggtctgagc tacatcgtcg
     3421 tcatgctgcc cagcatggcc gtgatgattc tcgtgctgat ggcgctgatc aaaaccctca
     3481 cgcacctgac cggcctgaaa ctcgacgacc tgctcgccga acatcttcag gaaccgaaaa
     3541 aagaaacaga atcagttatc agtgaatagt tatctgttat cagttattcg tgagccaagc
     3601 ttcttgagtg aataaccgat aacaaataac tgataaccgg gccggaagcc ctcctagatg
     3661 atcagtccgc cgtctttttt caaatcggcc ggcgtgatga tcttattggc ctgttcgcgc
     3721 tggtactgct gagcctcttc aatcatccgg ttcatcgcat cttccatcgc ctgcgggaac
     3781 ttcagaatgg cttcttccag cgtcttggcg tcgatcgtgc cttggagcgg cagcggaccg
     3841 tgcgggctca tcagctgcgt atgtccgctg aaaaacggac tgcgcgccgt gtcttccgag
     3901 ccatcgacct tgaccggcac cagtttgcgg ataccgccgg tccgcaggtc ggtgtaggat
     3961 tcttcgcggt agagattggc gatgtccatt ttgacatcac tcagtttttc gggctgggca
     4021 ctcataaggg ttcctttcgg gccgcctgcg gcggcggtta tcagttgtcc gttctcagtt
     4081 atccgggtta ttttcctgat agtttgtcac tgttaaccat caacaattaa ccgataactg
     4141 gactgcggat taagccatag aaggccggac ggggaaaccc t
//
LOCUS       DAOPZO010000147         4075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_28757_length_4075_cov_6.348259, whole genome shotgun sequence.
ACCESSION   DAOPZO010000147 DAOPZO010000000
VERSION     DAOPZO010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28757_length_4075_cov_6.348259"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..946)
                     /locus_tag="PKI68_08285"
     CDS             complement(32..946)
                     /locus_tag="PKI68_08285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNX53727.1"
                     /translation="MTEGVHPKIAVIIPTYNRWPHVCDAIESVLNQSYPAVEAVVVDD
                     ASTDGTAEKLAAKYGGRIRLIALKKNGEKSAARNAGILATDAAYVCMLDSDDLLTENS
                     VDDRMKLFSADPSFNGVAYGLIQVNGTLKRNLDVDPEGNVLETFVRHPGFINNNSWLT
                     SRSKLLEVGLFNEALTNMEDRELLLRLTAKLHFRCCKTVVQNVRRVDAVSARSNYDRL
                     IQQGKKYIDTVRGNEALVQRLGSLLVDLEFGEDKTISDALYKSGRYRDYCQCSRKMFS
                     QYGPKMWRIRFLRRHILSFLLSSITKGK"
     gene            complement(960..1613)
                     /locus_tag="PKI68_08290"
     CDS             complement(960..1613)
                     /locus_tag="PKI68_08290"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53728.1"
                     /translation="MKIVIPLQTCSTRVKNKNIRPFYGEKSLFDIKAEQILAFEKPEN
                     VYISSENPVVEGLCANYGFNFMLRDPSLCGNEIYQPDLVKALTDPIPGEDDILWIQVT
                     SPLFNDFARALKEWETAREKHDSLVAVKRFKGHLLDERANPVNYAFGYWHKVSQKLPP
                     LYTVLWSLFILKRETVRKFQYHIGVNPYLFVTDSTVVDIDYEEDFELAQIIYKKLQG"
     gene            complement(1610..2572)
                     /locus_tag="PKI68_08295"
     CDS             complement(1610..2572)
                     /locus_tag="PKI68_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53729.1"
                     /translation="MYKLKRKIHQFVVRRRAKRLGADWAHLAECHPSFSMERTETGHR
                     VFWKKKEVGFVQNMKSVKGTGKGPCFVVATGPSMKTLDLKDLRGQQTLSVNGAINLFL
                     ENGMVPDHVLIPDCRIFENCFEFVEKSVRSGANCFFSHTGLSRICERDPALLRQGRIF
                     LIEPIDNKYDQPLSSSGWPCSPEAVPPGIYLSPEFPDTRKRRGLMGFSCDAERGFFFG
                     RTVAYLSVQLAHWLGNDPIFILGMDLGGTGKAHCYAKTANGLPDFLRYYEPEIRVPFE
                     IAREAAVAENFAVYNLSEHSTLPASIIPKITVKEALALAKEVKS"
     gene            complement(2565..3353)
                     /locus_tag="PKI68_08300"
     CDS             complement(2565..3353)
                     /locus_tag="PKI68_08300"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HNX53730.1"
                     /translation="MQVVAASPEDRTFITAVAQRMQSICREAPGGWPACDPDGFRTLN
                     RGGRAGVAGLQLAGRKCCVKLFYDARLLIQWRNRLGFSKAKRAYRNGLELQRRGVNCP
                     AMLGWVQDWKSGRALLITDWADDARRIDHWLEEHGPDAATAQALGRFIRQMHDAGVLH
                     KDLSLRNLLMRAQGGGHEFLLLDYEDAGFYAAVSESQRLDNLHHLHERSLAAVPEDIR
                     REFLIAYLGGAAEVEAWCRKLNDLIAARPSKYTQTAGREGVRNV"
     gene            complement(3344..>4075)
                     /locus_tag="PKI68_08305"
     CDS             complement(3344..>4075)
                     /locus_tag="PKI68_08305"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HNX53731.1"
                     /translation="PEFVALCSPEGKRDNPEFEKIRDSRNTLLFRFHWNGKTFYHKEF
                     KSPTFGRQLRKRFRAFHQIRVIRALRRRGLNCPDVLCAGRKGIRIFCVYEGLAADGNA
                     PTLYRRIVDASETRISAEEFLRQFGLFTGTMHRRRVAHGDYQWGNVLVRFADDGLRFM
                     LIDNDRTSALTGLIYWYRLRNLIQLMHAADYVPAAHWEFFWNGYTEGYRKSLCWKSLI
                     ERTVRQRVAQRRLESAERKGARQCK"
BASE COUNT          927 a         1234 c         1037 g          877 t
ORIGIN      
        1 caacgatttc accagcacag aattattcat cttatttgcc cttcgtaatg cttgataata
       61 aaaacgagag aatatgccga cgtaaaaacc ggattcgcca catcttaggg ccatattgcg
      121 aaaacatctt ccggctgcac tgacagtaat cccgatatcg cccggattta tagagtgcat
      181 ctgaaatggt tttgtcctcg ccgaactcca gatcgaccag cagcgaacct agccgttgaa
      241 ccaacgcctc atttccccgg acagtatcaa tatatttttt tccctgctgg atgagccggt
      301 cgtagttgga gcgcgccgaa actgcatcca ctcgccgaac gttctgtacc accgtcttgc
      361 agcaccggaa atgcagcttc gccgtcaagc gcagcagaag ttcgcgatct tccatattgg
      421 tcaaggcttc attgaacaga ccgacttcca gcagcttaga ccgggatgtc agccacgagt
      481 tgttgttgat aaaaccggga tgcctgacaa atgtttccag aacattccct tcgggatcta
      541 catccagatt ccgcttcagc gtcccattca cctgaatcag accgtaggcg accccgttaa
      601 acgacggatc ggctgaaaac agtttcatcc ggtcgtctac cgaattttcc gtgagcagat
      661 catctgaatc cagcatacag acataggccg cgtccgtggc aagaatcccg gcattacggg
      721 cggccgactt ttcaccgttt tttttcagcg caatcagccg gatccggcca ccgtactttg
      781 ccgccagctt ttctgccgtt ccgtcggtcg aagcatcatc aacaaccacg gcttcaaccg
      841 ccggatagct ctgatttaaa accgattcga tggcatcgca gacatgcggc cagcggttat
      901 aggtcggaat aatgactgcg atcttcggat gtacaccttc agtcatgaag taacgatcct
      961 cagccctgaa gttttttata aataatctgc gccagctcaa aatcctcttc gtagtcaatg
     1021 tccacaaccg tggagtccgt gacaaagaga tagggattga cgccaatgtg gtattgaaac
     1081 ttcctgacgg tctcgcgctt cagaataaag aggctccaga gtacggtgta aagcggcgga
     1141 agcttctgcg aaaccttgtg ccaatatccg aaggcatagt tgacgggatt ggcccgctcg
     1201 tccagcaggt gacctttgaa ccgtttgacc gccaccagcg agtcgtgttt ttcccgcgcg
     1261 gtttcccact ctttcagagc gcgtgcaaag tcgttaaaca gcggcgaggt cacctggatc
     1321 caaagaatgt catcctcgcc cggaatcgga tcggtcaacg ctttgacgag gtcgggctga
     1381 taaatctcgt tcccgcacag cgacgggtcg cgcagcatga agttgaagcc gtagttggca
     1441 cacaaccctt caaccaccgg attttcggac gagatataaa cattctccgg cttttcaaaa
     1501 gccaggatct gctccgcttt gatgtcaaac agcgactttt cgccgtaaaa cggacggata
     1561 tttttatttt tgacgcgggt tgaacaggtc tgcaacggaa taactatttt caacttttca
     1621 cctctttcgc caacgcgaga gcttctttca ctgtaatttt cggaataatg gaggccggca
     1681 atgtactgtg ttccgacagg ttgtacacgg caaaattttc ggcaaccgcc gcttcgcggg
     1741 cgatttcgaa cggcacccgg atttccggct cgtaatagcg cagaaaatca ggcagcccgt
     1801 tggccgtctt cgcataacaa tgcgccttac cggttccgcc cagatccatg ccgagaataa
     1861 agatcgggtc attaccgagc caatgcgcca gctgaacact caaataggcc acggtccggc
     1921 caaagaaaaa accgcgctct gcatcgcagc tgaatcccat cagaccgcgc cgtttgcgcg
     1981 tatccggaaa ctccggcgaa agatagattc ccggcggaac ggcttccggc gagcacggcc
     2041 agccggatga agacaaaggc tgatcgtatt tattatcgat cggctcgatc aggaagatcc
     2101 gcccctgccg tagcagcgcg gggtcgcgct cgcagatccg cgacagaccg gtatgcgaaa
     2161 aaaaacagtt cgcgccggag cgcaccgact tttctacgaa ctcgaagcaa ttttcaaaaa
     2221 tccggcagtc gggaatcagc acatgatcgg gaaccatacc gttttccaaa aacagattaa
     2281 ttgcaccgtt caccgagagg gtctgctgcc cgcgaagatc cttgagatcc aatgttttca
     2341 tcgacggccc ggtggccacc acgaaacaag gtcccttgcc ggtgccttta acggacttca
     2401 tgttctggac gaacccgact tcttttttct tccagaacac ccggtggccg gtctccgtgc
     2461 gttccattga aaacgacgga tggcattccg ccagatgcgc ccagtcggct cccaaccgct
     2521 tcgcgcggcg gcgtacgaca aattgatgga ttttgcgttt cagtttatac attccgcacc
     2581 ccttcccgcc cggcggtctg agtatatttt gaaggccgcg cggcaatcag atcattcagt
     2641 ttccggcacc aggcttcaac ttcagctgca ccgccaagat acgcgataag aaattctctc
     2701 cggatatctt ccggaacggc ggccaaactc cgttcgtgca aatggtgcag attatccagt
     2761 cgctgcgact cggaaaccgc cgcgtaaaat ccggcatctt cataatccag cagcaaaaac
     2821 tcatgcccgc cgccttgcgc ccgcatcagc agattgcgca gcgaaagatc tttatgcagc
     2881 actccggcgt cgtgcatttg ccggataaac cgtcccagcg cctgcgccgt cgccgcatcc
     2941 ggcccgtgtt cttccagcca gtgatcgatc cgccgggcat cgtccgccca gtccgtaatc
     3001 agcagggccc ggccgctttt ccagtcctga acccacccga gcattgcggg gcaattgacg
     3061 ccgcggcgct gcaactccag cccgttccgg tacgcccgtt tggcttttga aaagcccagc
     3121 cggttgcgcc actggatcaa aaggcgggca tcgtaaaaca gtttgacgca gcatttccgt
     3181 ccggcgagct gcagcccggc aacgccggcc cgcccgccgc gattcagcgt ccggaacccg
     3241 tccggatcgc aggccggcca gccgcccggc gcctcgcggc aaatcgattg catgcgttgg
     3301 gcaaccgccg tgataaaggt ccggtcctcc ggagaagccg cgactacttg cattgccggg
     3361 ctcctttccg ctccgccgac tccagccgcc gttgcgccac ccgctggcgg accgtgcgct
     3421 caatcagcga cttccagcac aggctcttgc gatagccttc ggtatagccg ttccagaaaa
     3481 attcccagtg cgccgccgga acgtagtcgg ccgcgtgcat cagctggatg agattgcgca
     3541 ggcggtacca gtaaatgagc ccggtcagcg ccgaggtccg gtcgttatcg atcagcataa
     3601 accgcagacc atcgtccgca aaacggacca gcacattgcc ccactgataa tcgccgtgcg
     3661 ccacgcgccg gcggtgcatg gtgccggtaa acagcccgaa ctggcgcaga aactcttcgg
     3721 cagaaatccg cgtttccgaa gcgtcgacaa tccgccggta aagggtcggc gcgttgccat
     3781 cggcggccag cccttcatag acgcagaata tccggattcc tttgcgaccg gcgcaaagga
     3841 cgtccgggca gttcaagcct ctgcgccgga gagcccggat cacccggatt tgatggaacg
     3901 cccggaaccg cttgcgcaac tgccgcccga acgtgggcga tttgaattcc ttgtgataaa
     3961 acgttttgcc gttccagtga aaccggaaca gcagcgtatt gcgtgagtcg cgaatttttt
     4021 cgaactccgg attatcccgt ttgccttccg gactgcacag cgccacgaac tccgg
//
LOCUS       DAOPZO010000148         4046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_29075_length_4046_cov_10.139063, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000148 DAOPZO010000000
VERSION     DAOPZO010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29075_length_4046_cov_10.139063"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2443
                     /gene="polA"
                     /locus_tag="PKI68_08310"
     CDS             <1..2443
                     /gene="polA"
                     /locus_tag="PKI68_08310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNX53732.1"
                     /translation="AEKEGFDTYMVTPDKDFTQLVDAHTFVYKPAKSGLPPEIMGVPE
                     VLKYWEIERIEQVTDILGLAGDAADNIPGVPGVGPKTAQKLIARFGSIENLLDHTGEL
                     KGKLKENLEANKERARLCKWLVTIKTDSPLDYAPDGLLRGKPDEEKLAALFSEFEFSA
                     FSKRLFGKPAAPVAPVASYAPPPTKGGQGDLFDFASQQAAAAPAEDAAPVYKTIDDVK
                     HTYRLVQTLEERKALAAQLSQAPRFCFDTETTGLDPRTARLVGLSFAVKPHEAWFVTL
                     PPSETESAKALEIFRTLFEDERIEKTGHNLKFDIAMLRAAGIEVRGKLFDTMLAHYLL
                     DPDQRHGLDKLAGTHLGYAPVPITALIGEKKSGQLSMADVLVDKLSDYACEDADITLQ
                     LRHILEPQLEKRKLTNVFDTIECPLIPVLVEMEANGIALDTAALNEISGRLAGEIAGY
                     EKKVYELAGREFNLNSPKQLGEVLFDELKLVDKPKKTKTGQYTTNEEVLDQLATEHEI
                     ARVLLDYREATKLKNTYVDALPEFISKKTGRIHTSFSQAVTTTGRLASSDPNIQNIPV
                     RTEQGQEIRRAFVAGSSEFTLLAADYSQIELRIMAHLSGDQHMKAAFESGEDIHTATA
                     ARVFGVALADVTSDMRRKAKMVNFGIIYGISSFGLAQRLRIPRKEAADIIESYFKQYP
                     SVKACIDRIIETARSKGYVETIAGRRRHIRDINTANGTVRAAAERYAINAPIQGSAAD
                     MIKIAMIRIHDLLKQKNAKTKLLLQVHDELVFELHKDEHGLIEDIRTLMSDALPLSVP
                     VLVDCGTGTNWLEAH"
     gene            2512..3216
                     /locus_tag="PKI68_08315"
     CDS             2512..3216
                     /locus_tag="PKI68_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53733.1"
                     /translation="MKDVFVSRPTWVSEEFEDGLNKFIGFLKSKGLNPRTLGSTDYPT
                     ECPLDEVLGIMKQCCGAIILGYPQITISKGSVKNKVVTSELTLATEWNHIEAGLAHAR
                     GIPLLIIHHKNISRGIFDRGTMPNFIHSIDLANASWILSEPILGAFKKWETDINSQKP
                     PTAEDLNISFDEATGTLTCDESPYCFCHKCYHEKNNALVPLTKKNWGWRCQVCKEDYK
                     DPNYIPPPPRSSRRSF"
     gene            3539..>4046
                     /locus_tag="PKI68_08320"
     CDS             3539..>4046
                     /locus_tag="PKI68_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNX53734.1"
                     /translation="MKLFLFILLAVTILLFAVWRLHAGEMPAASANAIRVACVGDSIT
                     YGARIFNREAKCYPAQLGVLLGTNYSVQNFGVNGATLLKHGDLPYWQTRAFKAAQDFK
                     PQIAVINLGANDAAVYNWVYKYEFVKDYTELIRSLQSSGCAAVWICYPTPTYPGPYDF
                     TESVVVNEI"
BASE COUNT          994 a         1251 c         1029 g          772 t
ORIGIN      
        1 tgcggagaaa gaaggcttcg acacctacat ggtcacgccg gacaaagatt tcacccagct
       61 ggtcgacgcg cacaccttcg tctataaacc ggccaagagc ggcctgccgc cggaaatcat
      121 gggcgttccc gaagtactca aatactggga gatcgagcgc atcgaacagg tcaccgacat
      181 tctcggcctc gccggcgacg ccgccgacaa tattcccggc gtgccgggcg tcggcccgaa
      241 aaccgcgcag aaactgatcg cccggttcgg ctcgattgaa aacctgctcg accataccgg
      301 cgaactcaaa ggcaagctca aggaaaacct cgaagcgaac aaagaacgcg cccggctctg
      361 caaatggctc gtcaccatca aaaccgactc gccgctcgac tacgctcccg acggactgct
      421 ccgcggcaaa cccgacgaag aaaaactcgc cgcgctcttt tcggaatttg aattctccgc
      481 cttctccaaa cggctcttcg gcaaacccgc cgcgccggtt gcgcccgtcg cctcctacgc
      541 cccgccgccg accaaaggcg gacaaggcga tctcttcgac ttcgcttcgc agcaggcggc
      601 tgccgcaccg gcggaagacg ccgcgccggt ttataaaacc atcgacgacg tcaaacacac
      661 gtaccgcctc gtccaaacct tggaagaacg caaagcgctg gcggctcagc tgagccaggc
      721 gccgcgcttc tgcttcgaca ccgaaaccac cgggctcgat ccgcgcaccg cgcggctcgt
      781 cggcctttcc tttgcggtta aaccgcacga agcctggttt gtcacgctgc cgcccagcga
      841 gacggaatcc gccaaagcgc tggagatttt cagaacgctg tttgaggacg aacggatcga
      901 aaagaccgga cacaacctga agttcgacat cgccatgctg cgcgcagcgg gcatcgaagt
      961 gcgcggcaaa cttttcgaca cgatgctcgc gcattacctg ctcgatcccg accagcgcca
     1021 cggactcgac aagctggccg gcacgcacct cggctacgcg ccggtgccga tcaccgcgct
     1081 gatcggcgaa aagaaaagcg ggcagctgtc gatggccgac gtgctggtcg acaagctgag
     1141 cgactacgcc tgcgaagacg ccgacatcac cctccagctc cgccatattc tggagccgca
     1201 gctggaaaaa cgtaaactca ccaacgtatt cgacaccatc gaatgtccgc tcattccggt
     1261 gctggttgaa atggaagcca acggcatcgc gctcgacacc gccgcgctca acgaaatttc
     1321 cggacggctc gccggcgaaa tcgccggtta tgaaaagaag gtgtatgaac tggccggacg
     1381 cgagttcaac ctcaactcgc ccaagcagct cggcgaagtg ctcttcgacg aactcaagct
     1441 ggtggataag ccgaagaaaa ccaaaaccgg acagtacacc accaacgagg aagtgctcga
     1501 ccagctggcg accgaacatg aaatcgcccg cgtcctgctc gactaccgcg aagcgaccaa
     1561 actcaaaaac acctacgtcg acgcgctgcc ggaattcatc tccaaaaaaa ccggccgcat
     1621 ccacacctcg ttcagtcagg ccgtcaccac caccggacgg ctggcgtcga gcgatccgaa
     1681 cattcagaac attcccgtcc gcacggaaca gggacaggaa atccgccgcg ccttcgtcgc
     1741 cggctccagc gagttcactc tgctggccgc cgactattcg cagatcgaac tgcgcatcat
     1801 ggcgcacctc agcggcgacc agcacatgaa agccgcgttc gaaagcggcg aagacattca
     1861 caccgccacc gccgcgcgcg tcttcggcgt cgcgctcgcc gatgtcacct ccgacatgcg
     1921 ccgcaaagcc aagatggtca acttcggcat catctacggc atttcgtcgt tcggcctcgc
     1981 gcagcggctg cgcattccgc gcaaagaagc ggcggacatt atcgagagct atttcaaaca
     2041 gtatccgtcg gtcaaagcgt gcatcgaccg catcatcgaa accgcgcgca gcaaaggcta
     2101 cgtcgaaacc atcgccggcc ggcggcgcca catccgcgac atcaacaccg ccaacggcac
     2161 cgtccgcgcc gccgccgagc gctacgccat caacgcgccg atccagggct ccgccgccga
     2221 catgattaaa atcgccatga tccgcatcca cgacctgctg aaacagaaga acgccaaaac
     2281 caaactgctc ctgcaggtcc acgacgaact cgtctttgaa ctgcacaaag acgaacacgg
     2341 actcattgag gacatccgca cgttgatgag cgacgccctg ccgctatccg tccccgtcct
     2401 cgtcgactgc ggcaccggca ccaactggct tgaggcgcat taagtttttt gcccacgaat
     2461 cacacgaatg gacactaatt cggcagcccg aatcacgacg aaaggctaaa tatgaaagat
     2521 gtatttgtaa gtcgccctac atgggtttca gaagagttcg aggatggatt gaataaattc
     2581 atcggattcc tgaagtcaaa ggggctgaac cccagaaccc ttggatctac agattatcca
     2641 acggagtgcc cactagatga agttcttggc attatgaagc aatgctgtgg agcaattatt
     2701 ttagggtacc ctcaaattac tatttccaaa ggatcggtta aaaacaaagt tgtcacatcc
     2761 gaacttactc ttgctacaga atggaaccac atcgaagccg gattagctca cgctcgggga
     2821 ataccgctct tgattatcca tcataaaaat atcagtcggg gtatttttga tcgcggaaca
     2881 atgccaaatt tcattcactc aattgatcta gcaaatgcat cttggatatt gagcgaacct
     2941 attctgggcg catttaaaaa atgggaaact gatataaatt cccaaaagcc accgacagca
     3001 gaagacctta acattagctt cgatgaagcg acaggaaccc tcacttgcga tgaatcgcct
     3061 tattgttttt gccacaaatg ttatcatgaa aaaaacaacg ccttggttcc tctgacgaaa
     3121 aaaaactggg gctggcgctg tcaggtgtgt aaagaagatt ataaagatcc caattatatt
     3181 cctccgcccc caaggtcttc tcgacgcagc ttctaagcga cagcacgaag gcgaacggtc
     3241 aaagaccttc gcgccattct cggctctaag agccgactct acatttccgc tgattccatc
     3301 aaggccgcca ggatggcggc gatacgtacc gcaggcatcc tgcctgcctt gaaacggcgg
     3361 ttccttttaa aacaaaacgc gtccgccggt cgcgccagcc ctgagcttag tcgaagggtt
     3421 ccgccgacgg gttcatttcc ggaagcgctg ctgatgttcc gccccgcatt ttcgatttgc
     3481 catgcaataa aagcttttcg gctgcgtttt cctgaaatcg ttatcttgga accctgctat
     3541 gaaacttttt ttatttattc ttctggccgt aacgatcctg ctgtttgcgg tctggcggct
     3601 ccacgccgga gaaatgccgg cggcatctgc gaacgcaatc cgcgtggcat gtgtcggcga
     3661 cagcatcacc tacggcgcgc ggatttttaa ccgcgaggcg aagtgttatc cggcgcagct
     3721 cggcgtactg ctgggaacca attattcggt gcagaatttc ggcgtgaacg gcgcgacgct
     3781 gctgaagcac ggcgatcttc cgtactggca gacgcgtgcg ttcaaggcgg cgcaggattt
     3841 taaaccgcag atcgcggtga tcaatctcgg cgcgaacgat gccgcagtct ataactgggt
     3901 ttataaatac gagtttgtga aagactacac cgaactgatc cggagtcttc aatcgagcgg
     3961 ttgcgcggcg gtctggatct gctatccgac gccgacctat cccggcccgt atgatttcac
     4021 cgagtcggtt gtcgttaacg aaatta
//
LOCUS       DAOPZO010000149         4003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_29554_length_4003_cov_9.104610, whole genome shotgun sequence.
ACCESSION   DAOPZO010000149 DAOPZO010000000
VERSION     DAOPZO010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29554_length_4003_cov_9.104610"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PKI68_08325"
     CDS             complement(<1..704)
                     /locus_tag="PKI68_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HNX53735.1"
                     /translation="MSNQSAVINHQSAIKASAPGSLMLLGEHAVLHGRRALVCAINQR
                     ITVSLSQCAEKTLRIFSDLGTYEAPLGALTDHPDFRFVLDAVKQYPLTQGVELKIESE
                     FSSDIGFGSSAAVTVATHAVLMNNPPRPSLCSGHPSHGGDLLKSPPSEGCPQGGAGSF
                     DSIFLNELFKRALATIHRVQGRGSGADVAASVFGGVVAYRAEPLEIVPLRQSFPLTAV
                     YSGAKMKTADVIRWLE"
     gene            complement(770..1774)
                     /locus_tag="PKI68_08330"
     CDS             complement(770..1774)
                     /locus_tag="PKI68_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53736.1"
                     /translation="MKAVSPGKLILSGEHSVVYGKPAVAMAIDRSVTATIEDTADGQI
                     AFEMPGVSERESFTMLALQDFKRRALNNYRLFLEGKMGIRDVLHKPVDLFKFGFITVL
                     DGLHRSLDSGIVIKLKSSIPVGSGMGSSAASVLSELRALGHFLRVDFRPEWYYEFSLE
                     TERLQHGHPSGVDSYISLYGGCAAFQNGQARSVPLPRMKMYLIETGTPEATTGECVVQ
                     VKEQFQHDLIWNDFEAVTGEVEKAVRENNSALMRSLLRENHRLLCRIGVVPPKVQQFI
                     ADVETAGGAAKICGAGSVRGQAGGVVLVLADFMPVALADKYGYKVSPVRGDPLGTRIV
                     "
     gene            complement(1935..3089)
                     /locus_tag="PKI68_08335"
     CDS             complement(1935..3089)
                     /locus_tag="PKI68_08335"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HNX53737.1"
                     /translation="MKTGIDQISFYTSQYFIDLKTLAEARGVDPEKFYTGIGQEKMGI
                     PSPDEDVVTMAASAAFRLKEQGALDGVEALLFATESGIDQSKSAGLFVYGLLGLPERC
                     RVVELKQACYSATAALRMAMGLVAMKPQSKVLVIASDIARYELGSPGESTQGCGAVAF
                     TVSADPRLLAVDPEAGFYADDVMDFWRPNYLSEALVDGKYSTMVYIKALLESWKQYAA
                     ESGRSLDDFARFCYHIPFTRMAEKAHQKLAKGTSDEELRRVIGESLIYSRQAGNCYSA
                     SLYVGLASLLDNAAEDLSGKRIGLYSYGSGCVGEFFSGVVQENYRAALFAKQHRTLLA
                     GRTELTFQQYEDIYNYGIPVDGGEHTFPQYRTGPFRFAGMQGHKRIYEPA"
     gene            complement(3086..>4003)
                     /gene="fni"
                     /locus_tag="PKI68_08340"
     CDS             complement(3086..>4003)
                     /gene="fni"
                     /locus_tag="PKI68_08340"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HNX53738.1"
                     /translation="LTHRALPEIDLKAIDPSVEFMGKTLSFPLLISSMTGGGDKLFKK
                     INTNLAAAAEAEGVAMGVGSQRIFFTEPTAKSSFDLRGVAPSTLLFSNLGAVQLNEDM
                     TLQHARAVVQVLKADALCLHLNPLQEAIQPEGDTNFSGLLGKIGEIVQGLEVPVIVKE
                     VGAGISAADAKLLIEAGVKYIDVAGTGGTSWSRVEGERSEDASLGQLFQDWGIPTPLA
                     LKQLAPLSVTLIASGGIRSGVDMAKAVVLGASLCGLARPFLNPAMESVEAVRAVIQRL
                     RREFVTAMFLLGADRVEKIKGRAKLIVKP"
BASE COUNT          855 a         1220 c         1135 g          793 t
ORIGIN      
        1 tcttcgagcc agcggatcac atccgccgtc ttcatcttcg cgccggaata aaccgccgtc
       61 agcggaaagg attggcgaag cggcactatc tccagcggtt cggcgcgata cgccacgact
      121 ccgccgaaca cactcgccgc gacatccgcg ccggaaccgc gtccctgcac gcgatgaatg
      181 gtcgccaacg cacgtttgaa aagttcattc aaaaatatgg aatcaaaaga acccgccccg
      241 ccctgcgggc acccctccga cggaggggat tttaaaaggt cccctccatg ggaggggtgg
      301 ccggagcaaa gcgacggacg gggtgggtta ttcatcagca cggcgtgcgt ggcgacggtg
      361 acggcggcgg acgagccgaa gccgatgtcg ctggaaaatt ccgattcgat tttcagctca
      421 acgccctgcg tcagcggata ctgcttcacc gcgtcgagta cgaagcggaa atcgggatga
      481 tcggtcagcg caccgagcgg agcttcatag gttccaaggt ctgaaaaaat ccggagggtt
      541 ttctctgcac attgagaaag cgaaaccgta atccgttgat tgatggcgca gaccagtgcg
      601 cgtcggccgt gaagcacggc gtgttcgccc agcagcatca ggctcccggg agcggaagcc
      661 ttaattgctg attgatgatt gatgactgcc gattgattgc tcattaaaaa aacgcgcccg
      721 gaggtcgcgt tccacctttg tgctctttgc gttccttcgc ggttaatttc tacacgattc
      781 tcgttccgag cggatcgccg cggacgggcg agactttata gccgtattta tcagccagtg
      841 cgacaggcat gaaatcggcc agcacaagca ccacgccgcc ggcctggccg cgaaccgatc
      901 cggcaccgca gattttcgcc gcgccgccgg cggtttcaac atcagcgata aactgctgaa
      961 ccttcggcgg cacgacaccg atgcggcaga gcagccggtg gttttcgcgg agcagcgagc
     1021 gcatcagcgc actgttgttt tcgcggaccg ctttttcgac ttcgccggtc accgcttcaa
     1081 aatcgttcca gatcagatcg tgctgaaact gttctttcac ctgcaccacg cattcgccgg
     1141 tggtcgcttc gggcgtgccg gtttcaatga ggtacatctt catgcgcggc agcggcaccg
     1201 aacgcgcctg gccgttctgg aacgcggcgc atccgccgta gagtgaaatg taggaatcta
     1261 cgccgctggg atggccgtgc tgaagccgtt cggtttccag actgaattcg taataccatt
     1321 ccggccggaa atcgacgcgc aggaagtggc cgagcgcgcg cagttcgctg agtaccgacg
     1381 cggcggaaga gcccattccg ctgccgaccg gaatgctcga cttgagtttg atgacaatgc
     1441 cgctgtcgag cgaacggtgc agtccgtcga gtacggtaat aaatccgaat ttaaaaagat
     1501 cgaccggctt atggagcaca tcgcgaatac ccattttgcc ttcaagaaac aggcggtaat
     1561 tgttaagtgc gcggcgcttg aaatcctgaa gcgccagcat ggtgaacgat tcgcgttcgc
     1621 tgacgccggg catttcaaaa gcaatctgac catcggcagt gtcttcaatc gtcgccgtca
     1681 cgctgcggtc aatggccatc gccacggccg gctttccgta caccacggaa tgctcgccgc
     1741 tcaggatcag tttgcctgga gatacagcct tcattgccgg tgattagacc atcttcggct
     1801 taaaaacaca acgtcctgag ctcatacgaa ttccaggttc gccggctccg acattcgcta
     1861 gttgatattg gatattcggg cgccgtgaaa acaatcccaa cagcaaatgt ccaataacca
     1921 ataactccaa ccggctatgc cggttcgtaa atccgcttat gaccctgcat cccggcgaag
     1981 cggaaaggtc cggtgcggta ctgaggaaaa gtgtgttcgc cgccgtcgac cggaatgccg
     2041 tagttgtaaa tatcctcgta ctgctggaac gtcagttcgg tgcgtccggc aagcagcgtc
     2101 cggtgctgct tcgcaaacag cgcggcgcgg taattttctt gcacaacgcc gctgaaaaat
     2161 tcgccgacgc agccggagcc gtaactgtag agtccgatgc gtttcccgga cagatcctcc
     2221 gccgcgttgt cgagaaggga agccagtccg acatacagcg aagcggaata gcagtttccc
     2281 gcctgccggc tgtaaatgag cgattcgccg atcacacggc gcagttcttc gtcggaagtt
     2341 cccttcgcca gtttctggtg cgctttttcc gccatccggg taaacggaat gtggtagcag
     2401 aagcgggcaa agtcatcgag cgagcggccg ctctcggcgg catactgctt ccacgattcc
     2461 agcagcgctt tgatgtaaac catcgtcgag tacttgccgt ccaccagcgc ttcggaaaga
     2521 tagttcggcc gccagaaatc catcacatca tcggcataaa acccggcttc aggatcgacg
     2581 gccagcaggc gcggatcggc ggaaacggtg aaagcgaccg cgccgcagcc ctgcgtcgat
     2641 tcgccgggac tgccgagttc gtagcgggca atatcggaag caatcaccag cactttgctc
     2701 tgcggtttca tggcgacgag tcccatcgcc atgcgcagcg cggcggtcgc gctgtagcag
     2761 gcctgcttca gctccaccac acggcagcgc tccggcagac cgagcaggcc gtaaacaaac
     2821 agtccggcgg atttcgactg gtcgatgccg ctctcggtgg cgaacagcag cgcttcaacc
     2881 ccgtcgagcg cgccctgctc tttcagccgg aacgccgcgc tggcggccat cgtaacgaca
     2941 tcttcatccg gagaaggaat ccccatcttt tcctgcccga tgccggtata gaatttttcc
     3001 ggatcgacgc cgcgcgcttc cgccagcgtt ttcaggtcga tgaaatactg cgaagtataa
     3061 aacgaaatct ggtcgatgcc ggttttcatg gtttaacaat cagcttcgcg cggcctttga
     3121 ttttttcaac gcggtccgcg ccgagcagga acatcgccgt cacaaactcg cgccgcaagc
     3181 gctgaatcac cgcgcgcacc gcctcgacgg actccatcgc cggattcaga aacggccgcg
     3241 ccagcccgca aagcgacgcg cctagaacca ccgctttcgc catatccaca ccggaacgga
     3301 tgccgccgga agcaatcaat gtgacgctca gcggcgcgag ctgtttcagc gccagcggcg
     3361 tcggaatacc ccagtcctgg aaaagctgac cgagcgacgc atcttcgctg cgctcgcctt
     3421 cgacccggct ccacgacgtt ccgccggtac cggcgacgtc gatatacttt acaccggctt
     3481 caatcagcag tttcgcgtcg gcggcggaaa ttcccgcgcc gacttcttta acgatcaccg
     3541 gcacttccaa gccctggacg atctcgccga tttttcccag aagaccggaa aagttggtgt
     3601 cgccttccgg ctgaatcgct tcctgcaacg gattcaggtg cagacagagc gcatcggctt
     3661 tcagcacctg caccacggcg cgagcgtgct ggagagtcat atcttcgtta agctgcaccg
     3721 cgccgagatt ggaaaacagc agcgtggatg gcgcaacgcc gcgcagatca aagctcgatt
     3781 tggcggtcgg ctcggtaaaa aaaatacgct gggagccgac gcccatcgcc accccttcgg
     3841 cttcggcggc ggcggcgagg ttcgtgttga tcttcttgaa cagcttatcg ccgccgccgg
     3901 tcatcgacga gatcagcagc ggaaacgaaa gcgtcttgcc catgaactcg accgacggat
     3961 caatcgcctt caggtcgatt tccggcagcg cacggtgcgt cag
//
LOCUS       DAOPZO010000150         3913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_30582_length_3913_cov_10.541472, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000150 DAOPZO010000000
VERSION     DAOPZO010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30582_length_3913_cov_10.541472"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="PKI68_08345"
     CDS             complement(<1..1466)
                     /locus_tag="PKI68_08345"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNX53739.1"
                     /translation="MKANDYSVSMDALASLCKRRGFIFQTSEIYGGLNGFWDYGPLGV
                     ELKRNIKESWWRATVQSRENVVGLDSAIIMHPKVWEASGHIGNFKDPMVDCRETKGRY
                     RADQLLVFKHKTDAAALMFAYPEDEEAPEKKAKKAKANPADYESVPLAEIPLDAYDKL
                     VGPDTDKPGTLTEPRSFNLMFKTYVGPLEESSNVAWLRPETAQGIFAQFANVHSVARQ
                     KVPFGIAQIGKAFRNEINPRNYTFRSREFEQMELEFFIKPGTDAEWHEYWVSERLKWY
                     ATVGLPEGRMHRDVHEKDKLAHYASACTDIMYDFPFGTQELEGIAARGNFDLTQHQNA
                     SGKSLEYFDEESKEKFLPHVVEPSAGVDRIALALLCEAYREEWIAKDGTVTTAEPNKP
                     APEGCETRTVLRFAPCIAPYKVAVFPLLKNKEELVGKAREVFEKLNRRWNCFYDHAGA
                     IGRRYRRQDEIGTPCGVTIDFQTLEDNTVTLRDRDTMQ"
     gene            1536..1832
                     /locus_tag="PKI68_08350"
     CDS             1536..1832
                     /locus_tag="PKI68_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53740.1"
                     /translation="MKEKTTYRRFDLPAFLAILGCAGMLIFECIFIFELYDRTPSQVA
                     SLLPAQPARREPVEPAPAAIQTNAPAPVAPNALQLSAPVPPAGNEKIPAAAPVG"
     gene            1929..2555
                     /locus_tag="PKI68_08355"
     CDS             1929..2555
                     /locus_tag="PKI68_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNX53741.1"
                     /translation="MARRTKEDAQVTRESVLIAALDLFSEKGYSRTTFSDIAKKISMT
                     RGAVYWHFDNKLALLAAMVDYFCTLREERVRQHFSEIHSIEELKQSFIEHARAVAEDR
                     RLKQFEFFMAFQMEWSEELLTETHKILDKVRKDPFEKLVAILRSPVIAGRFRPEVDPG
                     SLAVTLAGLWVGLCKIYLGACPKIDLVSAAAEGFDCIMNGALKKEATV"
     gene            2552..3658
                     /locus_tag="PKI68_08360"
     CDS             2552..3658
                     /locus_tag="PKI68_08360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX53742.1"
                     /translation="MNKKTAAIVGIVIVSGLAGWFMHGLFGAKSGGMPPAGGRPAMPP
                     ATVVVQELSEKPLQLIDEYIAHVEPVQDVLVRTEVSGYIESVHFTEGSMVKEGDLLFT
                     IDRNSYQASVDAAAAELDRAQKQYDRMKNADVRSISKADMDSAESAFLRAKAALKLAQ
                     VSLNYTEIKAPVSGRIGAVQMTKGNYVTSASGPLARIVQVDPIRVVFSLPDHVYLTMR
                     QLELSGKAGSLVGKIRLPNGTVLPVAGKKDFDDNAISSNTGTMAVRYLFDNADGLLVA
                     GGYVKILLGNPEAPKGIAIPQRAVLADQQGSYVLTVDDAGKVSTARIKPGAQLGTDLI
                     VLSGLKAGDRIVTDGLQKAIPGSTVKMISAEEVK"
     gene            3655..>3913
                     /locus_tag="PKI68_08365"
     CDS             3655..>3913
                     /locus_tag="PKI68_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX53743.1"
                     /translation="MISRVFIDRPRLAGVISIVLMLAGILSIFSLPVAQYPQVTPPQI
                     TVSANYPGASAEVLADTVAGPLEDAVNGVDDMIYMSSTSDSS"
BASE COUNT          731 a          984 c         1282 g          916 t
ORIGIN      
        1 tgctgcatgg tgtcgcggtc gcgcagggtg acggtgttgt cttccagggt ctggaagtcg
       61 atggtgacgc cgcacggcgt accgatttcg tcctgacggc ggtagcggcg gccgatggct
      121 ccggcgtggt cgtagaagca gttccagcgg cggttgagtt tttcgaatac ttcgcgcgct
      181 ttgccgacga gttcctcttt gtttttgagc agcgggaaga cggcgacttt gtacggcgcg
      241 atgcacggcg cgaagcgcag gacggtacgg gtttcgcagc cttccggcgc gggcttgttc
      301 ggctcggcgg tggtgacggt tccgtctttg gcgatccatt cttcgcggta ggcttcgcag
      361 agcagggcga gggcgatgcg gtcaacaccg gcggagggct cgacgacgtg cggcaggaat
      421 ttttctttgc tttcttcgtc gaagtattcg agcgatttgc cgctggcgtt ctggtgctgg
      481 gtgaggtcga agttgccgcg cgcggcgatg ccttcgagtt cctgggtgcc gaaggggaag
      541 tcgtacatga tgtcggtgca ggcgctggcg tagtgggcca gtttgtcttt ttcgtggacg
      601 tcgcggtgca tgcggccttc gggcaggccg acggtggcgt accatttcag gcgctcggaa
      661 acccagtatt cgtgccactc ggcgtcggtg ccgggcttga tgaagaattc gagttccatc
      721 tgttcgaatt cgcgcgagcg gaaggtgtag ttgcgcgggt tgatttcgtt gcggaaggct
      781 ttgccgatct gcgcgatgcc gaacggtact ttctggcgcg cgacggagtg gacgttggcg
      841 aactgcgcga agatgccctg cgcggtttcg gggcgcagcc aggcgacgtt ggagctttct
      901 tccagcgggc cgacgtaggt tttgaacatg aggttgaagg agcgcggctc ggtgagcgtg
      961 ccgggcttgt cggtgtcggg gccgacgagt ttgtcgtagg cgtcgagcgg gatttcggcg
     1021 agcggcacgg attcgtagtc ggcggggttc gctttggctt tcttggcctt cttttccgga
     1081 gcttcttcgt cttcggggta ggcgaacatc agcgcggcgg cgtcggtctt gtgtttgaag
     1141 acgaggagct ggtcggcgcg gtagcggcct ttggtttcgc ggcagtcaac catcgggtct
     1201 ttaaagttgc cgatgtggcc ggaggcttcc cagactttcg ggtgcatgat gatggcgctg
     1261 tcgaggccga cgacgttttc gcggctctgg acggtggcgc gccaccagct ttccttgatg
     1321 ttgcgcttca gttcgacgcc gagcgggccg tagtcccaga agccgttcag gcctccgtag
     1381 atttccgagg tttggaagat aaagccgcgg cgcttgcaga gcgaggcgag ggcgtccatc
     1441 gaaactgagt aatcgttagc tttcataagg gggcagagga tgcccgaaac ggtttggagt
     1501 tgaaagttaa aatttaaaag ttgaggctgt gccgcatgaa agaaaaaacg acataccggc
     1561 ggttcgatct gcccgcgttt ctggccatac tgggttgtgc cggtatgctg atttttgaat
     1621 gcatttttat ttttgaactc tacgaccgta cgccttcgca ggttgcaagc ctgcttccgg
     1681 ctcagccggc ccgccgtgag ccggtcgaac cggcgcccgc ggcaattcaa accaacgcac
     1741 cggccccggt tgcgccgaat gctcttcagt tatccgcgcc ggttccgccg gccggaaatg
     1801 aaaaaattcc tgcggctgca ccggtcggct aagttttctt actttccaaa aaatcctgtt
     1861 tatctgtttg atatacattc acgaatgtat gtataagtga cgccctcaaa ccggcagaag
     1921 ggtggaaaat ggccagacga acaaaagaag atgctcaggt gacgcgggaa agcgtactga
     1981 ttgccgcgct ggatctgttc agcgaaaagg gctattcgcg cacaaccttc agcgatatcg
     2041 ccaaaaagat cagtatgact cgcggcgcgg tttactggca ctttgacaat aagctggcac
     2101 tgctggcggc gatggttgat tatttctgca ccctccgcga ggagcgggtc cggcagcatt
     2161 tttctgaaat tcattccata gaggagttga agcagtcttt catcgaacat gcccgggcgg
     2221 tggcggaaga ccggcgtctc aagcagtttg aattttttat ggctttccag atggagtggt
     2281 cggaagagct gctgaccgag acgcataaga tactcgataa agtccgcaag gatccgtttg
     2341 aaaagctggt ggccattctg cgctcgccgg tgattgccgg ccggttccgc ccggaggttg
     2401 atcccggcag tctggcggtt acgctggcgg ggctgtgggt cgggttgtgc aaaatttatc
     2461 tcggagcctg tccgaaaatt gatttggtct ccgccgcggc ggagggattt gactgcatta
     2521 tgaacggagc tttaaagaaa gaggcgacag tatgaataag aaaacagcgg cgatagttgg
     2581 aatcgtaatc gtgtccggtc tggccggatg gtttatgcac ggtctgttcg gtgcaaaaag
     2641 cggcgggatg ccgccggcgg gcggacgtcc ggcaatgccg cccgctacgg tcgtcgttca
     2701 ggagctcagc gagaaaccgc ttcagctgat cgacgaatat atcgcgcatg tcgagccggt
     2761 gcaggacgtt ctggtgcgta ccgaagtgtc gggctatatc gaatccgtac atttcaccga
     2821 aggcagcatg gtgaaagaag gggatttgct tttcacgatc gaccggaatt cgtatcaggc
     2881 ttctgtcgac gcggctgcgg ccgagctgga tcgtgcgcaa aagcagtatg accgtatgaa
     2941 gaatgcggac gtccgcagta tttcaaaggc ggatatggat tccgcggaga gcgcttttct
     3001 gcgcgccaaa gccgctttga agctggcgca ggtttcgttg aattacacgg aaatcaaagc
     3061 gccggtcagc gggcgcatcg gcgcggtgca gatgaccaaa ggaaattatg tcacgtccgc
     3121 atccggtccg ctggcgcgca ttgtgcaggt ggatccgatc cgtgtggttt tttcgcttcc
     3181 ggatcatgtg tatctgacga tgcgccagct ggagctgtcc ggaaaggccg gttcgctggt
     3241 cggtaaaatc cgtttgccga acggcaccgt tctgccggtt gccgggaaaa aagatttcga
     3301 cgataacgcc atcagcagca ataccgggac gatggcggtc cgctatctct ttgataacgc
     3361 ggacggactg cttgtcgcgg gcggttatgt gaaaatcctg ctcggaaatc cggaggctcc
     3421 gaaaggcatt gcaattcctc agcgggcggt tttggctgac cagcagggtt cgtatgtgct
     3481 gacggtggat gatgccggca aggtttccac ggcgcgcatt aagcccggcg cacagctcgg
     3541 taccgatctg atcgtgcttt ccggtctgaa ggccggcgac cgtatcgtga cggatggctt
     3601 gcagaaagcc attcccggct cgaccgtaaa gatgatttca gcggaggaag ttaaatgata
     3661 tcccgcgtat ttattgaccg tccgcggctg gcgggcgtga tttccattgt gctgatgctg
     3721 gccggtattc tttcgatttt ctcgctgccg gttgcccagt atccgcaggt aacgccgccg
     3781 cagatcaccg tatcggcaaa ctatcccggc gccagcgcgg aagttctggc cgataccgtg
     3841 gccgggccgc ttgaggacgc ggtcaacggc gtggacgaca tgatttatat gtcatccact
     3901 tccgacagtt ccg
//
LOCUS       DAOPZO010000151         3826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_31590_length_3826_cov_10.443914, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000151 DAOPZO010000000
VERSION     DAOPZO010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31590_length_3826_cov_10.443914"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PKI68_08370"
     CDS             <1..297
                     /locus_tag="PKI68_08370"
                     /inference="COORDINATES: protein motif:HMM:NF014856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HNX53744.1"
                     /translation="KEQSVCVVMAAGGYPGDYQKGDEISGLAAAAEHAVVFHAGTALK
                     DGKVVTAGGRVLGVTGIGKDLRAAVDNAYAAVQKISWNGAFCRKDIAHRALKRG"
     gene            376..1542
                     /locus_tag="PKI68_08375"
     CDS             376..1542
                     /locus_tag="PKI68_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNX53745.1"
                     /translation="MKRYVTLISGLLVAVSAHCAGEPWRFVSMPDFLNVDTDYPQKGW
                     EDSLSFILQSVKAEQPDFLVVPGDLVMGHWHKNDVEQGLPGIERFARQYYSAWKARMD
                     AAGLKWYAGIGDHEIGDNPWKYPEAPAYVQAYKAAFRQHLNMPLNGPEHMKGTAYWWR
                     HKNVLFVAVDVFEEGTSRQGSIRAGVTGPQLAWLKQVLAENRDAAHIIVMGHSPCLGP
                     VRTWSSSNLMIEEGRESPFWKTMAEYGVDLYLCGEVHAITCTERDGIQQVAHGGLIGY
                     NERTNYLLVEVFDDHLEFTLKEIDLVASGKKLWQPGENRPLEQVDITPEMRARGFIPV
                     GKETLDKTGSGKVFKNQQGCFLKKFEASAETGSPVFVKNHRPLPRINLDGSTSN"
     gene            1708..2214
                     /gene="purE"
                     /locus_tag="PKI68_08380"
     CDS             1708..2214
                     /gene="purE"
                     /locus_tag="PKI68_08380"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNX53746.1"
                     /translation="MAKPQVAIVMGSASDWKTVEACFQTLGEFGIKAEVKVMSAHRTP
                     HEACEFAENAAKNGFKVIIGAAGGAAHLAGVLAGHTILPVIGIPMPGWALDGMDSLLS
                     TVQMPKGVPVATVAIGKAGAINAAILAVQMLALSDAGLKKKLVSYKAQMRKEVLKSDA
                     DLQKQING"
     assembly_gap    2218..2317
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2318..2845
                     /locus_tag="PKI68_08385"
     CDS             <2318..2845
                     /locus_tag="PKI68_08385"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNX53747.1"
                     /translation="LLEAGELVVVPTETVYGIACDPAHLEKLYAAKERDRGKPVARLA
                     AGFEQVAALGANFGKHGLALAKKYWPGSLTLVLDTPDGPTGFRVPAHEVPLALARAFG
                     RPIALTSANKSGGADAVTAQEAFQTLEKQVALFLDAGECSGKVPSTVARCLESGVEIL
                     REGAVSRAEIEQAIV"
     gene            2842..3306
                     /locus_tag="PKI68_08390"
     CDS             2842..3306
                     /locus_tag="PKI68_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HNX53748.1"
                     /translation="MKKLIFVCTGNTCRSPMAEGLLKHLLGPECGWDISSAGVRASNG
                     FPASPGSTEALREKGIDISGHYSRRLTPEMIRKADLLVTMTAAHRDAILEISPESAGK
                     VFLLKSFGIAQCAADIYDPVGEALDVYRRVRDEIDAALPDLILYMMEERTDP"
     gene            3303..>3826
                     /gene="rpiB"
                     /locus_tag="PKI68_08395"
     CDS             3303..>3826
                     /gene="rpiB"
                     /locus_tag="PKI68_08395"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNX53749.1"
                     /translation="MKIVIGADHAGVELKTKFTALLKAKGFEVEDVGTHSAESVDYPD
                     YAAKVACRVSQGKADQGILICNTGIGMSITANKFPRVRAAVCTTAEMAKLTRIHNASN
                     VLCLGANNVTDPVSQEILEAWLANRPETGGRHERRVNKIKAFGKSTSDAIALYDSDPE
                     MYKILRDENVRQKN"
BASE COUNT          829 a         1041 c         1135 g          721 t
ORIGIN      
        1 aaagagcagt ccgtctgcgt cgtcatggcg gcgggcggct atcccggcga ctatcaaaaa
       61 ggcgacgaga tttccggcct cgccgcggcg gcggaacacg ccgttgtttt ccacgccgga
      121 accgcgctca aggacggtaa ggtcgtcacc gccggcggcc gcgtactcgg cgtcaccggc
      181 atcggcaaag atttgcgcgc cgcggtcgat aacgcctatg ccgccgtgca aaaaatttcg
      241 tggaacggcg cgttttgccg taaggacatt gcgcaccgtg cattgaagag gggttgattg
      301 ccaacagcgc gccgtccgca gtgaacgccg gaacagcctg tttcaattca ttcgttccgc
      361 caaggagtac acaggatgaa acgatatgtg acgcttatct cggggctgct tgtggcggtc
      421 tctgcgcact gcgcaggtga accctggcgg tttgtctcga tgcccgactt tctgaatgtg
      481 gataccgact atcctcagaa aggctgggag gattcgcttt cgttcattct gcaatctgtg
      541 aaggccgaac agcccgattt tctggtcgtg ccgggcgatc tggtgatggg gcattggcac
      601 aagaacgatg tcgagcaggg ccttccgggc attgagcgct ttgcccggca gtattattcc
      661 gcatggaaag ccaggatgga tgccgccggc ctgaagtggt acgccgggat cggcgatcat
      721 gaaatcggcg acaacccctg gaaatacccc gaagcgcccg cctatgttca agcctacaaa
      781 gccgcgttcc gccagcatct gaacatgccg ctgaacggac cggagcatat gaagggcacc
      841 gcttactggt ggcggcacaa gaatgttctg tttgtcgcgg tggatgtatt cgaagaaggg
      901 accagccgcc aaggctccat tcgggccgga gtcaccgggc cgcagctcgc ctggctgaag
      961 caggttctgg cggaaaaccg ggatgcggcg catatcatcg tgatgggcca ttcgccctgt
     1021 ctggggccgg tccggacctg gtcgagcagc aacttgatga ttgaagaggg ccgcgaatcg
     1081 cctttctgga aaaccatggc cgaatacggc gtcgatctct acctgtgcgg ggaagttcat
     1141 gccatcacct gcaccgagcg cgacgggatt cagcaagtcg cgcacggcgg actcattggc
     1201 tacaacgaac gcaccaacta tttgctggtg gaggtcttcg acgatcatct ggagtttacg
     1261 ctcaaagaga tcgacctggt ggccagcggg aaaaagctgt ggcagccggg cgagaaccgt
     1321 ccgctggaac aggtggatat cacacctgaa atgcgggccc gcgggtttat tccggtcggc
     1381 aaggaaacac tcgataagac gggctccggc aaggttttca aaaaccagca gggctgcttt
     1441 ctcaagaaat tcgaggcgtc tgcggaaacc ggctctccgg tttttgtgaa aaaccaccgc
     1501 cccttgccgc gaatcaatct ggatggctct acaagcaatt agaactcact acagacatcg
     1561 ccgcacctta aaggaattgc agtatgactc ggtttcaggc tatttctgtg caaacacgat
     1621 tggtagggtc tgcgaaagcc gacttccgcc cgcaaacgga ttccggcctg tcgtgattac
     1681 ttgaaacatc tgagaaggag acgaattatg gctaaaccgc aagttgcgat tgtaatggga
     1741 agcgccagtg actggaaaac ggtcgaagcc tgtttccaga cgctgggaga gttcggcatc
     1801 aaagccgaag tgaaagtcat gtcggcccac cgtacaccgc acgaagcgtg cgagttcgcg
     1861 gaaaacgccg ccaaaaacgg atttaaggtc atcatcggcg cggccggcgg cgcggcgcac
     1921 cttgcgggcg tgttggctgg ccatacgatt cttccggtga tcggaattcc gatgccgggc
     1981 tgggcgctcg acggaatgga ttcgctgctt tcgaccgtgc agatgccgaa gggcgtgccg
     2041 gttgccacgg ttgccatcgg caaagcgggc gcgatcaacg ccgcgattct tgccgtacag
     2101 atgctggcgc tttccgacgc cggactgaag aaaaagctgg tgagctataa agcccaaatg
     2161 cgcaaagagg tgctcaagtc cgacgccgat ttgcagaagc agatcaatgg ctgaaatnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctt ttagaggccg gcgaactggt
     2341 tgtcgttccg accgaaacgg tttacggcat cgcctgcgat ccggcgcatc tggaaaaatt
     2401 atacgccgcc aaggagcgtg accgcggtaa gccggttgcg cggctggccg ccggctttga
     2461 acaggtcgcg gcgctcggtg caaatttcgg gaaacacggg ttggcgctgg ccaaaaaata
     2521 ctggcccggt tcgctgacgc tggtgctcga tacgccggac ggcccgaccg ggttccgggt
     2581 tccggcccac gaagtcccgc tggcgctggc ccgcgcgttc ggtcggccga ttgcgctgac
     2641 cagcgcgaac aagagcggcg gagccgacgc cgttaccgcg caggaggctt tccagacatt
     2701 ggaaaaacag gttgctctgt ttctcgatgc gggcgaatgt tccggaaaag ttcccagcac
     2761 ggttgcccgg tgtctggaat ccggtgtaga aatcctgcgc gaaggagcgg tttccagagc
     2821 agagattgaa caggcgattg tatgaaaaag ctgatttttg tctgtaccgg caatacctgc
     2881 cgcagcccga tggccgaggg gctgctcaaa cacctgctcg gtcccgaatg cggctgggat
     2941 atttcctcgg cgggcgtccg tgcaagtaac ggatttccgg ccagcccggg atcgacggaa
     3001 gcgctgcgcg aaaagggaat cgacatctcc ggtcactaca gccggcggct gaccccggag
     3061 atgatccgca aagccgattt actcgttaca atgaccgccg cccaccggga tgcgatcctg
     3121 gaaatttccc ccgaaagcgc cggtaaagtt ttcttgctaa aatcgttcgg aatcgcacaa
     3181 tgcgcggctg atatttacga cccggtcggg gaggcgctgg acgtttaccg tcgtgtacgc
     3241 gacgaaatcg atgccgcttt gcccgacctg attctttata tgatggaaga gaggacggac
     3301 ccatgaagat cgttatcggt gcggatcacg caggcgttga gctgaagacc aaatttacgg
     3361 cccttctgaa ggcaaaaggg tttgaagtcg aagacgtcgg aacccattcc gccgaatcgg
     3421 tcgattatcc ggattacgcc gccaaggtgg cctgccgggt ttcgcaggga aaagcagatc
     3481 aagggattct gatctgcaac accggtatcg gcatgagcat taccgccaac aagttcccgc
     3541 gcgtccgcgc cgccgtctgc acaacagcgg aaatggcgaa gctgacccgc attcataacg
     3601 catctaacgt cctctgcctc ggcgccaaca acgttacgga ccccgtctct caggaaattc
     3661 tcgaagcctg gctggcgaac cggccggaaa ccggcggacg ccacgagcgg cgcgtcaaca
     3721 agatcaaggc tttcggcaag agcaccagcg acgcgatcgc gctttatgat tccgatccgg
     3781 aaatgtacaa aatcctgcgc gacgaaaacg tccggcagaa aaacaa
//
LOCUS       DAOPZO010000152         3798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_31930_length_3798_cov_10.391130, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000152 DAOPZO010000000
VERSION     DAOPZO010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31930_length_3798_cov_10.391130"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1883)
                     /gene="glgB"
                     /locus_tag="PKI68_08400"
     CDS             complement(<1..1883)
                     /gene="glgB"
                     /locus_tag="PKI68_08400"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HNX53750.1"
                     /translation="MEARPKLTDFDLFLHGKGTHYRTYEKFGAHLLEEDGKKGVYFAV
                     WAPNAQHVSVIGSFNNWEAWIHKMTPISNSGVWELFIPGVKEGDQYKFEIKGPHGFWA
                     QKADPYAFAAEVRPRTASVVFDLGRYQWNDAEWINQRAQTKWHERPVSVYEVHMGSWK
                     RKVEEGNRWLTYRELAEQLVPYAREMGYTHIELMPIAEHPFDGSWGYQTSGYYAPTSR
                     FGTPDDFRFFVDRCHQAGIGVILDWVPAHFPKDAHGLGYFDGTHLYEHADPRMGEHMD
                     WGTLIFNYGRNEVSGFLLSNAVFWAHEYHIDGLRVDAVASMLYLDYSRKAGQWIPNRH
                     GGRENLEAIEFIKHFNSMVHAECPGFLTFAEESTAFPQVSHPVDFGGLGFDFKWNMGW
                     MNDTLKYFSKDPVHRKYHQGDLTFSMIYAFTENFILPFSHDEVVHGKASLLDKMPGDF
                     WQKFANQRLLFAYMFAHPGKKLLFMGCEIGQWREWDAATSLDWNLLAYDPHRKLKTLV
                     AKLNGLYTAEAALYDIESSWQGFDWIDFHDAASSVVSFLRKGRDPQEIIVCVLNLTPT
                     PRPGYRIGLPQAGDWKVILNTDAATYGGSNAGPKEEAVYAAHHLPWHGKGFSIQLDLP
                     PLGA"
     gene            complement(1886..3781)
                     /locus_tag="PKI68_08405"
     CDS             complement(1886..3781)
                     /locus_tag="PKI68_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53751.1"
                     /translation="MSDWIGHECGIAAIRLLKPFEYYVEKYGSASFAINRLYLLMEKQ
                     HNRGQDGAGAAVVKLDVEPGKPFIFRSRSADKDPIAKVHQEMLTSLAESQSGNPEHAN
                     DPLWLKRNAKFAGELLLGHLRYGTRGRGGIDFCHPFLRPSNWRSRNLVLAGNFNLTNN
                     DELFEALVDLGQHPIDETDTVMVLEKIGHFLDEENDRLFREFRKEGLERAVISEKIAD
                     HLDLVTVLKRAVKNFDGGYAMAGIIGNGDLFVMRDACGIRPGFFYRDDEFVVVASERP
                     AIQTAFDVPIDAVQEIKPGHALIVRRNGEVLHEQISETQEIRQCSFERIYFSRGTDRD
                     IYGERKQLGRQLTEPVLKAVDYDLDNTVFSYIPNTADAAFYGLMEGAKAYVAERAKYH
                     ILHERNLTAERIVELVNYQPRMEKIMTKDAKLRTFITADSDRRELVSLVYDTTYGVVK
                     KTDTLVILDDSIVRGTTLRESILRILDRLQPKKIVIVSSAPQIRYPDCYGIDMSKLKD
                     FVAFQAVVALVKENGREAVLKQIYEACKASLNLPREQVKNEVKALYAPFTPAQIEKKI
                     AQILRPADLKAQFQVIYQSIEGLRAACPKHTGDWYFTGDYPTSGGNRVVNRSFVNFME
                     NSDARAY"
BASE COUNT          752 a         1096 c         1118 g          832 t
ORIGIN      
        1 gcgccgagcg gcggcaggtc gagctggatg gagaatcctt taccgtgcca cggcaggtgg
       61 tgcgcggcgt agaccgcttc ttctttcggg ccggcattgc ttccgccgta ggttgcggcg
      121 tcggtgttga gaatgacttt ccagtcgccg gcctgcggca ggccgatgcg gtagccgggg
      181 cgcggcgtcg gcgtaaggtt gagaacgcag acgatgattt cctgcgggtc gcggcctttg
      241 cgcaaaaagg aaacaaccga acttgccgca tcgtgaaaat caatccagtc gaagccctgc
      301 caggatgatt caatgtcgta gagcgcggct tcggcggtgt agagaccgtt cagtttcgcc
      361 accagtgttt tgagtttccg gtgcgggtcg taggccagca ggttccagtc gagactggtc
      421 gcggcgtccc attcgcgcca ctggccgatc tcgcagccca tgaagagaag tttcttgccg
      481 ggatgcgcaa acatataggc aaagagcagg cgctggttgg cgaacttctg ccagaagtcg
      541 ccgggcattt tgtcgagcag cgaggctttg ccgtgaacca cttcgtcgtg cgagaacggc
      601 aggatgaagt tttcggtgaa ggcgtagatc atggagaacg tcaggtcgcc ctgatggtat
      661 ttgcggtgaa ccggatcttt gctgaaatat ttcagcgtgt cgttcatcca gcccatattc
      721 cacttgaagt cgaagccgag gccgccgaag tcgaccggat gagagacctg cgggaaggcg
      781 gtggattctt cggcaaaggt gagaaatccg gggcattccg catgcaccat gctgttgaag
      841 tgcttgatga attcaatcgc ttcgaggttt tcgcgtccgc catggcggtt gggaatccac
      901 tgccccgctt tgcgcgaata gtcgagatag agcatgctgg cgacggcgtc cacgcgcagg
      961 ccgtcgatgt gatattcatg cgcccagaaa acggcgttgg agagcaggaa gccgctgact
     1021 tcgttgcgtc cgtaattgaa gatgagcgtg ccccagtcca tatgttcgcc catccgcgga
     1081 tcggcgtgtt cataaaggtg cgtgccgtca aaatagccga ggccgtgcgc gtctttcgga
     1141 aagtgcgccg gaacccagtc gaggatgacg ccgattccag cctgatggca gcggtcaacg
     1201 aaaaaccgga aatcatcggg cgttccgaaa cgcgaagtcg gcgcatagta cccgctggtc
     1261 tgatagcccc acgatccgtc aaacggatgc tcggcaatcg gcatcagttc gatatgcgta
     1321 taacccattt cccgggcata cggaaccagc tgttccgcca gctcgcggta cgtcagccag
     1381 cggttgcctt cttccacctt gcgcttccac gagcccatat ggacttcata aacgctgacc
     1441 ggccgctcgt gccatttggt ctgcgcccgc tgattaatcc attcggcatc gttccactga
     1501 tagcggccca ggtcgaaaac caccgacgcg gtgcgcgggc gcacttcggc ggcgaaagcg
     1561 tacgggtcgg ccttctgcgc ccagaagccg tgcggccctt tgatttcaaa tttatactgg
     1621 tcgccttctt taacgccggg aatgaaaagc tcccagacgc cggaattgct gatcggcgtc
     1681 atcttatgga tccacgcttc ccagttattg aacgaaccga tcacgctgac gtgctgggca
     1741 ttcggagccc agaccgcgaa atagacgccc tttttcccgt cctcttccag caggtgcgcc
     1801 ccgaactttt cataggtccg gtaatgagtt cctttaccgt gcaggaacag gtcgaagtcc
     1861 gtcagtttcg gtcttgcttc cataatcagt aggcccgcgc gtcgctgttt tccatgaagt
     1921 tgacaaagga acggttgacg acgcggttgc cgccggaggt cggatagtcg ccggtgaagt
     1981 accagtcgcc ggtatgtttc gggcaggcgg cgcgcaggcc ttcgatgctc tggtagatta
     2041 cctggaactg cgccttgagg tcggcgggcc gcagaatctg cgcgatcttc ttctcgatct
     2101 gtgcgggtgt gaacggcgcg taaagcgctt tcacctcgtt tttcacctgc tcgcgcggca
     2161 ggttaagact cgctttgcag gcttcgtaaa tctgcttcag cactgcttcg cgcccgtttt
     2221 ccttcaccag cgcgaccacc gcctgaaagg cgacgaagtc cttgagcttg gacatgtcga
     2281 tgccgtagca gtccggatag cggatctgcg gcgcggagga gacgatgacg attttcttcg
     2341 gctggagccg gtcgagaatc cgcagaatac tttcgcgcag cgtggtgccg cgcacaatcg
     2401 agtcgtccag aatcaccagc gtatcggttt tcttcacgac gccgtaggtc gtatcgtaaa
     2461 ccagcgagac cagctcgcgc cggtcggaat cggcggtgat gaaggtgcgc agtttggcgt
     2521 ccttcgtcat gatcttttcc atgcgcggct gatagttgac cagctcgaca atccgttccg
     2581 cggtcagatt gcgttcgtgc aggatatgat atttcgcccg ttccgccaca tacgccttgg
     2641 cgccttccat cagtccgtaa aatgcggcat cggcggtgtt cggaatgtag gaaaaaaccg
     2701 tgttgtccag atcgtagtcc accgccttca gcacaggctc ggtgagctgg cggcccagct
     2761 gcttgcgctc gccgtaaatg tcgcggtcgg ttccgcgcga aaaatagatg cgctcgaacg
     2821 agcactgccg gatttcctgt gtttctgaaa tctgctcatg cagcacttcg ccgttgcggc
     2881 ggacaatcag cgcgtggccc ggtttgatct cctgaaccgc atcaatcggc acatcgaatg
     2941 ccgtctggat agccggccgc tcggacgcca ccaccacgaa ctcgtcgtcg cggtagaaaa
     3001 agccggggcg gatgccgcag gcgtcgcgca tcacaaacag gtcgccgtta ccgataatgc
     3061 ccgccatcgc gtagccgccg tcgaaattct tcacggcgcg cttcagcacc gtcaccagat
     3121 caaggtgatc cgcgatcttt tcggaaatca ccgcgcgctc aagaccttcc ttgcggaact
     3181 cgcggaagag ccggtcgttc tcctcgtcga ggaaatggcc gatcttctcc agcaccatca
     3241 ccgtgtcggt ttcatcaatc ggatgctgtc ccagatcaac cagcgcttca aaaagctcat
     3301 cgttgttggt caggttgaag tttccggcca gcaccagatt gcggctgcgc cagttgctcg
     3361 gacgcaggaa cggatggcag aaatcaatgc cgccgcggcc gcgggttccg tagcgcagat
     3421 ggccgagcag cagttcgccg gcaaacttcg cgttgcgctt cagccagagc ggatcgttcg
     3481 cgtgctccgg gtttcccgac tgcgattccg ccagcgaggt cagcatttcc tgatgaacct
     3541 tggcaatcgg atctttatcg gcggaacggg agcggaaaat aaacggctta cccggttcaa
     3601 cgtccagttt aacaaccgcc gcaccggcgc cgtcctgccc gcggttatgc tgcttttcca
     3661 tcagcagata gagccggttg attgcaaaag aagctgaacc gtatttttcg acgtaatatt
     3721 cgaacggttt cagcaagcgg atagcggcga tgccgcattc atggccaatc cagtcactca
     3781 tatcccatac actccttg
//
LOCUS       DAOPZO010000153         3796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_31961_length_3796_cov_8.069767, whole genome shotgun sequence.
ACCESSION   DAOPZO010000153 DAOPZO010000000
VERSION     DAOPZO010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31961_length_3796_cov_8.069767"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="PKI68_08410"
     CDS             complement(<1..1062)
                     /locus_tag="PKI68_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNX53752.1"
                     /translation="MKKAILLTVMLALTARGDDFAFKLYGQLRTAEGNLFFSPASIES
                     ALAMTREGAAGNTLRQMSLLLPEASAFPNTGTNVTLKSANAIWVDKQFPILAKFQSAV
                     QGKYGAEIRPADFAGQPEIERGTVNRWVEEKTRDKIKNLLAPGSVTPDTRLILVNAIY
                     FKGDWLHAFEKAKTEDEPFWMTPEVSTNVPMMTMKPERFSYTETESFQCLELPYQGED
                     VSMLIILPKEKDGLARIEDGLSSDALADWTGTFRKEEIEIHLPRFKIESQFDSMAKTL
                     AALGLTDAFNPALADFSGISARPLFISDVVHKAFVLADEKGTEAAAATGVAMCATALP
                     MSPKVFRADHPFLFLIRHNA"
     gene            complement(1064..2236)
                     /locus_tag="PKI68_08415"
     CDS             complement(1064..2236)
                     /locus_tag="PKI68_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNX53753.1"
                     /translation="MKNLIDQKRAVQNLMDLLAAEGPSGGEGPVADVVKQQLLNAGCR
                     PEWIQENVSRKRLPPNFTVGNLVVKIPGTVKAPRIMFSAHLDTVPICRGAVPVKKGNR
                     IVPKGKTALGGDNRASVAAIVTMADTVLKNNLPHPPLTLLFPVGEESGLHGAKAFKPS
                     EGGNPAMCFNLDGSGERGGVIGALGAIRWIAEVRGKSVHAAVNPEGGVSSAVIAANAI
                     AELKKEGWFGRINKGACKGTANVGTLSGGEVDNQVMDQLKVTGEWRSHSSASLKKIAT
                     VWKKAFEKAARGIKNNQGECGSVEFFTASDYRSFRLKNNEPVVQRFLKAAPKAGRKAY
                     TEVISAGLDANIFNEIGIPTITFDAGNHNAHDLNEYIDIPRYLEACTLATVLATEG"
     gene            complement(2307..>3796)
                     /locus_tag="PKI68_08420"
     CDS             complement(2307..>3796)
                     /locus_tag="PKI68_08420"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNX53754.1"
                     /translation="ATGLAGLLTASGTTAFAAPAARPNVVLIYGDDVGYGDSGVYGAT
                     KIPTPNIDRLARNGLLFTDGHCSASTCTPSRFSLLTGRYAFREDIAILKGDATLCIQP
                     ESYTMADMFRAAGYKTGVIGKWHLGLGNGEVDWNRTIAPGPQELGFDYSFIMPATVDR
                     VPCVYVENGRVVRLDPNDPIRVSYSEKITDTYPDGVDHPEAMTYYRSTPGFRHDNSVI
                     NGIGRIGWMSGGRAALWNDENLTIDTLRQTKQFITENKNQPFFLYFASQDIHVPRTPH
                     KRFQGATTLGPRGDAMVQFDWATGEILKMLEENGLLQNTLVIFSSDNGPVYKDGYEDG
                     CTVRLSDEESDHGHDGSGVFRGGKYKTYEGGTRVPFIVSWPGTVAPGTSSALISQVDL
                     LASFAALTGQTLPAGAAIDSQNRLDTLLGKNPRGDDVIIEQANGNILSVRQGDWKYIA
                     GKKGPELYDLKTDIGEQHNVAAQQPETVKQMAALLETYRTRGLRE"
BASE COUNT          645 a         1082 c         1190 g          879 t
ORIGIN      
        1 ggcgttatgc cggatcagga agaggaacgg atggtcggcg cggaatactt tcggtgacat
       61 gggcagagcg gttgcacaca tggcgacccc ggttgcggcg gcggcctcgg tgcctttttc
      121 gtccgccagc acaaacgctt tatgcaccac gtcggagata aacagcggcc gggcgctgat
      181 tcctgaaaag tcggccagcg ccggattgaa ggcatcggtc aggcccagcg cggcgagagt
      241 tttcgccata ctgtcgaact gcgattcgat cttaaagcgc ggcagatgaa tttcgatttc
      301 ttctttgcga aaagtgccgg tccagtccgc cagcgcatcg gaagagagtc cgtcttcaat
      361 acgggcgaga ccgtctttct ccttcggcag gataatcagc atggaaacgt cttcgccctg
      421 atagggaagc tccaggcatt gaaagctttc agtttccgtg tagctgaaac gctccggctt
      481 cattgtcatc atcggcacgt ttgtgctgac ttccggcgtc atccagaacg gctcatcttc
      541 tgtttttgct ttttcgaacg cgtgcagcca gtcgccttta aagtagatcg cattgaccag
      601 aatcaggcgc gtatccggtg tcacggatcc cggtgccagc aggtttttga tcttatcgcg
      661 ggttttctct tcgacccagc ggttgacggt tccgcgttcg atttcaggct gaccggcgaa
      721 atcggccggc cggatttccg cgccgtattt tccctgcacg gcggattgga attttgcgag
      781 gattggaaac tgtttatcca cccagatcgc gttggcgctt ttcagcgtca cattggttcc
      841 ggtattcggg aaggcggacg cctccggcag cagcaggctc atctgccgga gcgtattgcc
      901 ggccgcgcct tcgcgcgtca tggccagtgc ggactcaatg ctggccggtg aaaaaaacag
      961 atttccctcc gccgtgcgca gttgaccgta gagcttaaac gcaaagtcat cgccacgcgc
     1021 ggtcagtgcc agcatgacgg tcagcagaat ggcttttttc atttcaaccc tccgtagcga
     1081 gaaccgtcgc cagtgtgcag gcttcgaggt agcgcggaat atcaatatat tcgttgaggt
     1141 cgtgggcgtt gtggttgccc gcgtcgaagg taatcgtcgg gatgccgatt tcgttgaaga
     1201 tgttggcgtc gagtccggcg ctgatcactt cggtataggc tttgcggccg gccttgggcg
     1261 cggctttcag gaagcgctgc accaccggtt cgttgttttt aagccggaag ctccggtagt
     1321 cgctggcggt gaaaaactca acggagccgc attcgccctg attgtttttg attccgcggg
     1381 ccgctttttc gaacgccttc ttccaaaccg tcgcgatttt tttcagcgaa gccgagctgt
     1441 ggctgcgcca ttcgcccgtt accttcagtt gatccatgac ctggttgtcg acctcgccgc
     1501 cggacagcgt gccgacattc gcggttcctt tgcaggcgcc tttattaatc cgtccgaacc
     1561 agccttcttt tttcaattcc gcaatggcat tggccgcaat cacggccgag gaaacgccgc
     1621 cttccggatt tacggcggca tgcacactct tgccgcggac ttcggcgatc cagcggatgg
     1681 cgcccagcgc gccgattacg ccgccgcgtt cgccgctgcc gtcgaggttg aagcacatcg
     1741 ccggatttcc accttcagac ggcttaaacg ctttggcacc gtgcaggccg ctctcttcgc
     1801 cgaccgggaa gagcagggtg agcggcggat ggggcaggtt gtttttcagt acggtgtcgg
     1861 ccatggtgac gatggcggcc accgaggcgc ggttgtcgcc gcccagcgcg gttttgcctt
     1921 tcggaacaat ccggttgcct tttttgaccg gtacggcgcc gcggcagatg ggcaccgtat
     1981 ccagatgtgc ggaaaacata atgcgcgggg ctttgaccgt gcctggaatt tttacgacca
     2041 ggttgccgac ggtgaagttc ggcggcaggc gcttgcggct gacattttcc tgaatccatt
     2101 ccggacggca gcccgcgttc agcagctgct gcttcaccac gtctgccacc ggtccttcgc
     2161 cgccgctcgg cccttccgcc gccagcaggt ccatgaggtt ttgtacggcc cgtttctgat
     2221 caatcaaatt tttcatgaga cggaagatac ctcatcgacc gagcggaatg cgaatcgttt
     2281 gcgcgctttt gcgcggggcg ctgcggctat tcccgcagtc cgcgggtgcg gtacgtttcg
     2341 agcagcgccg ccatctgttt tacggtttcg ggctgctgtg cggccacgtt gtgctgttcg
     2401 ccgatgtcgg ttttgaggtc gtacaattcc ggcccttttt tcccggcaat gtatttccag
     2461 tcgccctgcc gcaccgacag aatgtttccg ttcgcctgtt caataatcac gtcgtcgccg
     2521 cgcggatttt taccgagcag ggtgtcgagc cggttctggc tatcgatggc ggctccggcg
     2581 ggcagggttt ggccggtgag ggcggcgaag gaggccagca ggtcaacctg gctgatgaga
     2641 gcgctggagg ttccgggggc aaccgtgccg ggccagctga caataaaagg aacccgggtt
     2701 ccgccttcat atgttttgta tttgccgccg cggaatacgc cggagccgtc gtggccgtgg
     2761 tccgactctt cgtcggacag gcgcacggtg cagccgtctt cgtagccgtc tttataaacg
     2821 gggccgttgt cgctggagaa gatgaccagc gtgttttgca gcaggccgtt ctcttccagc
     2881 attttcagaa tctctcccgt ggcccagtcg aactgcacca tggcgtcgcc gcggggtccg
     2941 agggtggttg cgccctggaa gcgtttatgc ggggtgcgcg gaacgtggat atcctgcgaa
     3001 gcgaagtaga ggaaaaacgg ctggtttttg ttttccgtga tgaactgttt cgtctgccgg
     3061 agcgtatcga tggtcaggtt ttcgtcattc cagagtgccg cgcgcccgcc cgacatccag
     3121 ccgattcgcc cgatgccgtt aatgacggag ttgtcgtgcc tgaagccggg ggtgctccgg
     3181 tagtaggtca tggcttccgg atggtcgacg ccgtcgggat aggtgtctgt gattttctcg
     3241 ctgtagctca cgcgaatggg atcgttcggg tcgaggcgaa ccacgcggcc gttttcaaca
     3301 tagacgcagg gcacgcggtc gacggtggcg ggcatgataa agctgtaatc aaagcccagt
     3361 tcctgcggac cgggcgcgat ggtccggttc cagtcgacct ctccgttgcc aagccccagg
     3421 tgccacttgc cgatcacgcc ggtcttgtag cccgccgcgc ggaacatgtc ggccatggta
     3481 tacgattcgg gctggatgca cagggttgca tctcccttta gaatggcgat gtcctcccgg
     3541 aaggcgtagc gcccggtgag cagcgagaag cgggacggcg tgcaggttga cgcgctgcag
     3601 tgtccatcgg tgaagagcag gccgttgcgg gccagccggt cgatgttcgg cgtggggatt
     3661 ttggtggcgc cgtagacgcc ggaatcgccg tacccgacat cgtccccgta aatcagaacc
     3721 acattgggcc gggcggccgg cgcggcgaac gcggttgtgc cggaggccgt cagcaatcct
     3781 gcgagaccgg ttgctg
//
LOCUS       DAOPZO010000154         3739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_32664_length_3739_cov_6.184039, whole genome shotgun sequence.
ACCESSION   DAOPZO010000154 DAOPZO010000000
VERSION     DAOPZO010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32664_length_3739_cov_6.184039"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1909
                     /locus_tag="PKI68_08425"
     CDS             <1..1909
                     /locus_tag="PKI68_08425"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNX53755.1"
                     /translation="KRWSLESLPFIPDEFKLKLIGNRGVDEQFQVIKTLCEQAEEIVC
                     ATDAGREGELIFRYILALSGCEQKPIRRLWLNSLTPDAIKEAFKNLKDGHDYDPLYAA
                     ARCRSESDWIVGLNATRYYTVRHGRFGGGTDRVLWSIGRVQTPVLAMIVGRDDAIDQF
                     RSQPFWELTTKYREVMFKYTGKRFEKPELAQVLLEKITGQPFEVTGIDGKEKKEPPPQ
                     LFDLTTLQRDVNKAHGLSAADTLAATQNLYESKLVTYPRTDSRYLNKDMKPRIPQILE
                     QLKAIRAEQIAPLNLAKLPFTSRIIDDKKVTDHHAIIPTGVEPRGLGANEQLVYDAIV
                     TQFIAAFYPACLKTLTTVDGVSNEVPFQTKGTQIIEPGWTALFPKKKKKADDEEDDQT
                     LPAFEKGETGPHEPSVREGKTNPPKHFTENSLLGAMEAAGKFVEDDALREALKERGIG
                     TPATRAAIIETLLSRNYIRREKKLLLATDMGRCLIALIRDPLLKSPEMTGEWEEKLKQ
                     IERQKLDAGEFMSGICSYIRGLIQTGASGRLDASRMGNCPLCGKEVIKGRTAYGCSGW
                     KEGCAYVLSPDYKGLSLTPNQIQVLLQLHILPYPVQIEGEPRLLILSTQGLPMDLLLP
                     SADWQKKEKSL"
     gene            complement(1917..2801)
                     /locus_tag="PKI68_08430"
     CDS             complement(1917..2801)
                     /locus_tag="PKI68_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53756.1"
                     /translation="MVQQLNLAQKWAVNGIMPSPKEACALLRGKLTVKQVGDTSVIEI
                     SAASPDAAEAVAIANEVARAYKKYVEGIMLAEKQRPAEALANTLKEQEDKAQKAEQHY
                     RELRKKQGLPERPVAVDKDAKRLRLTQLRMLAKCEMLEAQAKLDRLTTLNDEDLVTSF
                     TSYVNDPSIKTFDSEIQDIQKQLQSMAGDPATKQTQAALDELNQRIKTVMREFIKALA
                     ADCAEKKSKFEAFDQERAALSGQNENLETKRAGIEWDTQKSIFETLKKRVSEMPPPEE
                     KPPRCTVEIISLANTASD"
     gene            complement(2820..>3739)
                     /locus_tag="PKI68_08435"
     CDS             complement(2820..>3739)
                     /locus_tag="PKI68_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53757.1"
                     /translation="AEAVAVANETARVFADDIKEKNDAAAKIAQETAQRELQIQEELV
                     SQLKLKVQALRKKEGFSETLFTDEEEASLQKLQMEQLKKDLLSAEQEMLAMQERLKNL
                     TELKDKSLEEFATAIKDPFLDSILEQIRQHEKQLNEITPSLRADHIEVKKIQAALDKL
                     NGKLASAVEGLLKGTQAQYIVAKSKLDALDEWICKASLSSSAKSPELQQAQAEYETQQ
                     AALATLKAKSITTRPALPDCSVRIREFAVAHPSEFEMACQAVRPEPIKELHHATARIS
                     VQKQEEPIKGETPGMKPFSPLAIERAMIA"
BASE COUNT          805 a         1036 c          974 g          924 t
ORIGIN      
        1 gaaacgctgg tcgctggaat cgcttccgtt tattccggac gaatttaaat taaagttgat
       61 cggcaaccgc ggtgtagacg agcagttcca ggtgattaaa accctgtgcg agcaggcgga
      121 agaaattgtc tgcgcgaccg acgccgggcg ggaaggggag ttgattttcc gctacatcct
      181 cgcgctgagc ggctgcgaac agaaaccgat ccgccgcctc tggctcaact cgcttacgcc
      241 cgacgccatc aaagaggcct tcaaaaatct gaaggacgga cacgactacg atccgctcta
      301 tgccgcggcg cgctgccgca gcgagtccga ctggattgtc ggcctcaacg ccacgcgcta
      361 ttacacggtc cggcacggac gcttcggcgg cggaaccgac cgcgtactct ggagtatcgg
      421 ccgcgtgcag acgccggtgc tggcgatgat tgtcgggcgc gacgacgcca ttgatcagtt
      481 ccgctcgcaa ccgttctggg agctgacaac gaagtatcgc gaggtgatgt tcaagtacac
      541 cggcaaacgc tttgaaaaac cggaacttgc gcaggttctg ctcgaaaaaa tcaccggcca
      601 gccgtttgaa gtgaccggca tcgacggcaa agaaaaaaaa gaaccgccgc cgcagctttt
      661 cgacctcacc acgcttcagc gcgatgtaaa caaagcgcac ggactttccg ccgccgacac
      721 gctggctgcc acacagaatc tttacgaatc caagctggtt acctatccgc gaaccgactc
      781 gcgctatctc aacaaggata tgaaaccgcg cattccgcag attcttgaac agctcaaagc
      841 aatccgcgcc gaacagatcg cgccgctcaa cctcgccaaa cttccgttca ccagccggat
      901 cattgacgac aaaaaggtga ccgaccacca cgccatcatt cccaccggcg tcgagccgcg
      961 cggcctcggc gcaaacgaac agctggttta tgacgcaatc gtcacgcagt tcatcgccgc
     1021 cttctatccc gcctgtttga agacgctcac caccgtggat ggcgtcagca acgaggttcc
     1081 ctttcagacg aaaggcacac agattattga acccggctgg acggcgctgt ttccgaagaa
     1141 aaaaaagaaa gcagacgacg aagaggatga tcaaacgctg cctgcttttg aaaaaggcga
     1201 aaccggtccg cacgaaccgt cagtccgtga agggaaaacc aatccgccga aacacttcac
     1261 cgagaactcc ctgctcggcg cgatggaagc cgccggaaaa tttgttgaag acgacgcctt
     1321 gcgcgaagcg ctcaaagagc gcggtatcgg aacgcccgct acccgcgcgg cgatcatcga
     1381 aacgctgctt tcccggaact atatccggcg cgagaaaaaa ctgctgctgg ccaccgatat
     1441 gggacgctgc ctgatcgcgc tgattcgtga tccgctgctc aagtcgcctg aaatgaccgg
     1501 cgagtgggaa gaaaagctca aacagatcga acggcagaag ctggatgccg gtgaatttat
     1561 gagcggcatc tgcagctaca tccgcggact gattcaaacc ggcgcctccg gacggctgga
     1621 cgcttcgcgg atgggcaatt gcccgctgtg cggcaaagag gtcattaagg gccgcacggc
     1681 ctacggctgt tccggctgga aagaaggctg tgcgtatgtt ctatcgccgg attataaagg
     1741 cctgtcgcta acgccgaacc agattcaggt tttgctgcaa ctgcacatcc tgccgtatcc
     1801 ggtgcaaatt gaaggcgaac cgcgcctgct cattctttcc acacaaggcc tgccgatgga
     1861 tctgctgctt ccttccgctg attggcagaa aaaggaaaaa agcctttgag tttctcttaa
     1921 tctgacgctg tattggccag cgaaataatt tcgacggtac agcgcggcgg tttttcttcc
     1981 gggggtggca tctccgagac tctttttttg agcgtctcaa agattgattt ttgagtatcc
     2041 cattcgattc ccgctctctt ggtttcgagg ttttcatttt ggccggaaag tgccgcacgt
     2101 tcctgatcaa acgcctcaaa ttttgacttt ttctccgcac agtctgccgc gagcgccttt
     2161 ataaattctc tcatgactgt ttttatacgc tggttcagct cgtccaaggc cgcttgggtc
     2221 tgttttgtgg cgggatcacc cgccatggat tgaagttgtt tttggatgtc ttgaatttcg
     2281 gagtcaaacg tcttaatgga cgggtcgttt acgtaagaag taaacgacgt caccagatcc
     2341 tcgtcgttca gtgttgtcaa ccgatccagt ttcgcttggg cctcaagcat ctcacattta
     2401 gccagcattc ggagttgcgt gagccggagc ctctttgcgt ctttatcaac cgcaacaggt
     2461 ctttcgggta agccctgttt ttttcggagt tctcggtagt gctgttctgc tttctgagcc
     2521 ttatcttcct gttctttcaa cgtatttgcc agcgcctctg ccgggcgctg tttttccgct
     2581 aacatgatcc cttcaacgta ttttttatat gcacgggcaa cttcgtttgc aatcgcaacc
     2641 gcctctgcgg catctggact tgccgctgag atttcaataa cggaagtgtc tccgacctgc
     2701 ttcacggtca gttttcctct gaggagcgca caggcttcct tgggagaagg catgatgccg
     2761 ttgaccgccc atttctgggc gagattgagt tgttgtacca cgttggtcag gaagggaccc
     2821 taggcaatca tcgccctttc tatcgctagt ggtgagaagg gtttcatgcc gggcgtctcg
     2881 cctttgatcg gttcttcctg tttctgcacc gaaatccgcg ctgtggcatg gtgaagttct
     2941 ttgatgggct ccggtctgac ggcttggcat gccatttcaa attccgaggg atgtgccact
     3001 gcgaactccc ggatcctcac tgaacagtcc ggaagagccg gtcgagtcgt gattgatttt
     3061 gccttaaggg tggcgagggc ggcctgttgg gtttcatatt cagcctgggc ctgctgcagt
     3121 tcggggctct tggcagacga agataaggat gctttacaaa tccattcatc cagcgcatcc
     3181 agcttgctct tcgcgacaat atactgtgcc tgtgttccct tcagcaggcc ttccacagcg
     3241 gatgccagct tcccattcaa cttatcgaga gcggcctgaa tctttttgac ttcaatatga
     3301 tccgctctaa gggatggtgt tatttcattc aactgttttt catgttgccg aatctgttca
     3361 aggatggagt caagaaaagg atcttttatt gcggtcgcaa actcctctag cgatttgtct
     3421 ttaagctctg tcagattttt cagccgctct tgcattgcaa gcatttcctg ttcagctgaa
     3481 agcagatcct ttttgagttg ttccatctgg agtttttgca gggaagcctc ctcctcatcc
     3541 gtgaaaaggg tttcggagaa tccttccttt ttgcgcaatg cctgaacctt cagttttaac
     3601 tggctgacga gctcctcctg aatctgtaac tcccgttggg ccgtttcctg tgcgatcttt
     3661 gctgcggcat cgtttttctc cttaatatcg tcagcaaata cgcgggccgt ttcattggca
     3721 acggccaccg cttcggcag
//
LOCUS       DAOPZO010000155         3708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_33098_length_3708_cov_9.942239, whole genome shotgun sequence.
ACCESSION   DAOPZO010000155 DAOPZO010000000
VERSION     DAOPZO010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33098_length_3708_cov_9.942239"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1556
                     /locus_tag="PKI68_08440"
     CDS             <1..1556
                     /locus_tag="PKI68_08440"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HNX53758.1"
                     /translation="FKGPIGGRYDGGWSAGNGYIATHLQTTLFYPLLFGRHTGADYFG
                     SPWYMNLGKYFSYMAPPGYPGGASGDRDEFYGNSFYDISKFMRVIRPGNPYDHRVWAM
                     GASSTRDFAGDEFMTGVEWFLLPDLRSQPLPDVTAPVVALDKGAWFPDTGDVALYSQP
                     DDLSNTVRVITRATPWGMVGHGHACNNAFNITYGGQKVFYRTGYYTGAGDPFSVENYR
                     HTRAHNSILPYGTVTQSMDTEGYGWMPRFVAGKHLSYSLGDASHAYSGSFARSVCNPT
                     ISGRSLTIADGYADPGVTRFRRHLALLETGTVVVYDELEAQTAIPWTFRLNSPGPMVK
                     VGSNGVATSCSNGFAVAELYCDTPVTVGVTDLFDSGPVSAYAFLTTVWGRRDWLSGAN
                     QWHANFTPTNALQKTRFLTLVHRVQNGEPLVQSVQSLSSSGRQVISSGGWHVEAELDA
                     GKPSFLKIWDNAGTAMLVSGQATTNVQLGAGTYTAQQPGSTMLMELGQPVQEVVDTLP
                     NVVRYGNLY"
     gene            1868..1950
                     /locus_tag="PKI68_08445"
     tRNA            1868..1950
                     /locus_tag="PKI68_08445"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1901..1903,aa:Leu,seq:taa)
     gene            1971..>3708
                     /locus_tag="PKI68_08450"
     CDS             1971..>3708
                     /locus_tag="PKI68_08450"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HNX53759.1"
                     /translation="MDLNQLTGNPTEQALLAELIHAGQEHLFAGWDAPGIHDEAKAAF
                     LQSLARINGSYPGGLTGYISNARKLLAEAKEGKNPFEGFVPHQPDKVDLTQFNAAYDH
                     YEALGKNQFSKTAVVLVAGGLGERLGYSGIKLDIPVEVTGITPYLAHYAACLKAMEAR
                     MAKPRPVPFIIMTSDDTNAKTIAAFEANNWFGLKQEQVHILKQELVPAIADNAGRLAL
                     AEKYKLILKPHGHGDIHMLLYTSGLAARLAAQGIEHLLFIQDTNGQVFNAMPAALGVS
                     VEKGFDFNSIAVNRIPGEAVGGLAKLVKGETELTLNVEYNQLDPLLRATVSPQGDVPN
                     EQGFSMFPGNINVLVIRMASYVKILERTRGIIAEFVNPKYADAAKTAFKSPTRLETMM
                     QDLPKLFGPGEKTGVTVFDRRWAFSANKNNIKDAAAKHAAGGPPESGATAESDFYLAG
                     RMKLAAAGMNVETAKEELILGIPFTPGPRVLLRPSFAMTLEEVREKIKGGSIAADATL
                     ILDGQEITLENVEIAAGSALVIKAADGAKVAVKNLKVENDGFELVKLSAEEQNSPATP
                     EYLRLRGYRIVNR"
BASE COUNT          834 a         1177 c         1016 g          681 t
ORIGIN      
        1 atttcaaggg gccgatcggc gggcgctatg acggcggctg gtcggcaggc aacggctata
       61 tcgcgacgca tttgcagacc acgctgtttt accctctgct gttcgggcgg cacacaggtg
      121 ccgactattt cgggtcgccg tggtacatga atctggggaa atatttttcc tacatggcgc
      181 cgcccggtta ccccggcgga gcctccggcg accgcgacga attttacggc aactcatttt
      241 atgacatttc gaagttcatg cgcgtcatcc gtccgggcaa cccgtatgac caccgcgtct
      301 gggcgatggg cgcctcctcg acaagagact ttgccgggga tgaatttatg accggcgtcg
      361 aatggtttct gctgcccgat ctgcgaagcc agccgctgcc ggacgtaaca gcgcctgtcg
      421 tggcgctgga caagggcgcc tggtttccgg ataccggcga tgtcgcgctc tactcgcagc
      481 cggacgatct ctcaaacacg gtccgcgtga ttacccgcgc cacgccgtgg ggcatggtgg
      541 gccacggcca cgcctgcaac aacgcgttta atatcaccta cggcggccag aaagtatttt
      601 accggaccgg ttactatacg ggagccggcg atccgttttc ggtggaaaac taccggcaca
      661 cccgtgcgca caacagcatc ctgccgtatg gcaccgtgac gcaaagcatg gacaccgaag
      721 gctacggctg gatgccgcgc tttgtggccg ggaagcacct gagctacagc ctcggcgatg
      781 catcgcatgc ctatagcggc agttttgccc gctctgtctg caacccgacg atttcgggcc
      841 gcagcctgac gatcgccgac ggttatgccg atcccggagt gacgcggttc cggcgccatc
      901 tggcgctgct ggaaaccgga acggtggtcg tctatgacga actcgaagcg caaaccgcaa
      961 ttccctggac cttccggctc aactcgccgg gcccgatggt taaggtgggg tcgaacggcg
     1021 tggcgacgag ctgcagcaac ggctttgccg tggccgagct ctattgcgac acgccggtaa
     1081 ccgtcggggt gactgatctg tttgatagcg gacccgtttc tgcctatgcc tttctgacga
     1141 ccgtatgggg gcgccgcgac tggctgagcg gtgccaatca gtggcatgca aactttacgc
     1201 ccaccaatgc actgcaaaag acgcgcttcc tgacgctggt tcaccgcgtg cagaacggcg
     1261 agccgctcgt gcagtcggtc cagagccttt cctcgtccgg gcggcaggtg atttcctcag
     1321 gcggctggca cgtggaagcc gagctggatg cgggcaaacc gtcgttcctg aaaatctggg
     1381 acaatgccgg aacagcaatg ctggtgagcg gccaggccac tacgaatgta cagctgggcg
     1441 ccggcaccta tacggcgcag cagcccggca gtacgatgct gatggagctg ggacagccgg
     1501 tacaggaagt ggtggatact ctccccaatg ttgttcgcta cgggaatctt tactgaagga
     1561 gttccgcaca ccacttgacc gccgttcgta aatgaaggga acagctgcga tacaagacac
     1621 cggtcgaaaa ccgcaatatc ttttgttatt aaccccgtcc tctgattcgc ggcggcaaag
     1681 agttcctttt cgattgcgcc cgcgtccttt acacatatat ttttcaaatc gtagctagat
     1741 atgatggaaa aattcctatt tgatcctatc ctcaaattta aaagacagac aatctgggat
     1801 aaactccggc gtaatcggca ggcagatttg gattttaaat catttattat aaatgagtta
     1861 tattaaagcc cgggtggcga aatggtagac gcaagggact taaaatccct tgaccgaaag
     1921 gtcgtgcggg ttcgagtccc gccccgggca ccgcttttaa ggaacaacgc atggatctca
     1981 accaactgac cggaaacccg actgaacagg ctttgcttgc cgaactgatc cacgcaggac
     2041 aggaacatct cttcgccggc tgggacgcgc ccggaatcca cgacgaagcc aaagccgctt
     2101 tcctgcaaag tctcgcccgg atcaacggct cctaccccgg cggactgacc ggctacattt
     2161 ccaacgcccg caaactgctc gccgaagcca aagaaggtaa aaatccgttc gaagggttcg
     2221 tgccgcacca gccggataaa gtcgatctga cgcagttcaa tgccgcgtac gatcattacg
     2281 aagcgctcgg aaaaaatcag ttcagcaaga ccgcggtcgt tctcgtcgcg ggcggactcg
     2341 gcgagcggct cggctattcc ggcatcaagc tcgacatccc ggtcgaagtg accggaatca
     2401 caccctacct cgcccactac gccgcctgcc tcaaagccat ggaagcgcgc atggccaagc
     2461 cgcgcccggt gccgttcatc atcatgacct ccgatgacac caacgccaaa accatcgccg
     2521 cgttcgaagc caacaactgg ttcggcctga aacaagaaca ggtgcacatt ctcaaacagg
     2581 aactcgttcc ggctatcgcc gacaacgccg gacgcctcgc gctggcggaa aaatataaac
     2641 tgatcctcaa gccgcacggc cacggcgaca tccacatgct gctctacacc agcggcctcg
     2701 ccgcccggct cgccgctcag ggcatcgaac acctgctctt catacaggac accaacggac
     2761 aggtcttcaa cgccatgccc gccgccctcg gcgtatccgt cgaaaaaggg ttcgacttca
     2821 actccattgc cgtcaaccgc atccccggcg aagccgtcgg cggactggcc aaactggtca
     2881 aaggcgaaac ggaactgacg ctcaacgtcg aatacaacca gctcgatccg ctgctgcgcg
     2941 ccaccgtcag cccgcagggc gacgtaccga acgaacaggg cttctccatg ttccccggca
     3001 acatcaacgt actggtcatc cggatggcct cctacgtcaa aattctggag cgcacccgcg
     3061 gcatcatcgc cgaattcgtc aacccgaaat acgccgacgc cgccaagacc gccttcaaga
     3121 gcccgactcg cctcgaaacc atgatgcagg atctgccgaa actcttcggt cccggcgaaa
     3181 aaaccggcgt caccgtcttc gaccgccgct gggccttctc cgccaacaaa aacaacatca
     3241 aagacgccgc cgccaaacac gcggccggcg gaccgccgga atccggcgcg accgccgaat
     3301 ccgacttcta cctcgcgggc cgcatgaaac tcgccgccgc cggaatgaac gtcgaaaccg
     3361 ccaaagagga actgattctc ggcataccgt tcacacccgg accgcgtgtt ctgctgcgcc
     3421 cgtcgttcgc catgacgctg gaggaagtcc gcgaaaaaat caaaggaggc tcgattgccg
     3481 ctgacgccac gctcatcctc gacggccaag agatcacgct cgaaaatgtc gagattgcgg
     3541 ccggttcagc cctcgtcatc aaagccgccg acggcgcgaa agtggccgtc aaaaatctga
     3601 aagttgaaaa cgacggcttc gaactggtga aactttcagc cgaagaacag aacagccccg
     3661 ccacaccgga atacctgcgc ctgcgcggct accgcatcgt caaccgcg
//
LOCUS       DAOPZO010000156         3706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_33119_length_3706_cov_9.573815, whole genome shotgun sequence.
ACCESSION   DAOPZO010000156 DAOPZO010000000
VERSION     DAOPZO010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33119_length_3706_cov_9.573815"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="PKI68_08455"
     CDS             <1..1304
                     /locus_tag="PKI68_08455"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNX53760.1"
                     /translation="MKNFILDTNVLLHDPTAIHHFTACNVIIPLKVLEEIDQFKRELS
                     ERGRNARTVSRALDELRRQGRLSDGVTLAHGGTIKVTFSDKENPFAVGLQADDLILKM
                     AMDVQKAQPDERCTVVSKDINMRLKADALGLEAEDYETDKTREADEIYTGQTVLDADS
                     EAISFFAREGHAVIPGMETLFANQYVTLRNLRDHRQTMLARYDAETCRLVKLLTMGDG
                     APVVPRNREQRFAMDALLNDRIKLVTLSGKAGTGKTLLAIAAGYHQVMDTKEYKRLLV
                     SRPIFPMGRDLGYLPGTIEEKLDPWMQPIHDALELVVHSRAGAAQKKGGDLLAANPLI
                     DVEPLTYIRGRSIPNQFMIVDEAQNLTPLEVKTVITRVGHDTKIVLTGDLYQIDNPYV
                     DSMSNGLSAVVDKFRGEPLAAHMLLTEGVRSEIAEKAANLL"
     gene            1402..1752
                     /locus_tag="PKI68_08460"
     CDS             1402..1752
                     /locus_tag="PKI68_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53761.1"
                     /translation="MKKILAARGYHHCKTDDEFTRHGNHARNIQYAFSKEFHRNHETI
                     VDIVFLGADMAGEKLVIGFFRENVSIKNGIMESSDVSIPLSKFSLAEFEKQLDKLIRK
                     GNPFRTGVDGGESF"
     gene            1776..2999
                     /locus_tag="PKI68_08465"
     CDS             1776..2999
                     /locus_tag="PKI68_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNX53762.1"
                     /translation="MMDAQKLFAERIGGENYGKSTEIYKFEKIKRAKAKARKLHPDLE
                     ILDFGVGEPDGIAPAPIREALKVEVDKPSNRGYADNGIPEFKQAAAEYMKKFFGVTLD
                     PVAEINHSIGTKPALAMLPLAFVNPGDVIFQTVPGYPVMATHTRYLGGKVVDIPLLAE
                     NGFLPDLKKIDPALADRCKLFYINYPNNPTGAVATDEFYDELIAFAQKHNILIIQDAP
                     YATLVYDSPRKSILQRPGAKDCALELHSMSKAYNMTGWRLAFFCGAAWAVDALAMIKD
                     NCDSGQFKAIQHAACKGIADRSLAEAICKHYEQRLRRMVEVLRGVGFDAKMPGGTFYL
                     YVKAPKAAGSVQFKNAEEVSLYLIENFGISTVPWDNTGPFIRFGAVFESTGDADDERV
                     LSELSARLKKADLKF"
     gene            complement(2996..3547)
                     /locus_tag="PKI68_08470"
     CDS             complement(2996..3547)
                     /locus_tag="PKI68_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53763.1"
                     /translation="MNSQLKHLLEEYAALERGVQELVSAQCREVCELCTACCCRADLC
                     EEALESPFLRAVHGKDELDSDRYGFLTETGCALETGRPPVCYEFFCDELQAAQPDELH
                     RSVLLILGRLPAYAGEKAHGDAHLAEIMQAEELDHLAFQRLEKQMQNAREALDCIRAF
                     YNDGTLPEHSRKVLSRITASTEN"
BASE COUNT          859 a          978 c         1065 g          804 t
ORIGIN      
        1 atatgaaaaa ttttattctg gataccaacg tccttcttca cgaccccacc gccattcatc
       61 actttacggc ctgcaatgtg attattccgc tgaaggtgct ggaggaaatc gaccagttta
      121 aacgcgaact cagcgagcgc gggcgcaatg cccgcacggt ttcacgggcg ctggatgagc
      181 tgcgccggca ggggcggttg tcagacgggg tgacgctggc acacggcggc acgatcaaag
      241 ttactttttc cgacaaagaa aatccgtttg ccgtcggtct gcaggccgac gatctgattc
      301 tgaagatggc gatggatgtg cagaaggcgc agccggacga acgctgcacg gtggtcagta
      361 aagacattaa tatgcggctg aaggccgacg cgctgggact ggaagctgaa gactacgaga
      421 cagataaaac ccgtgaagcc gacgaaattt acaccggaca aacggtgctg gacgccgact
      481 cggaagcaat cagctttttt gcccgcgaag gccatgccgt gattcccggt atggagacgc
      541 tttttgccaa tcagtatgtg acgctgcgta atttgcgtga tcaccgtcag acaatgctgg
      601 cacgctatga cgcggaaacc tgccggctgg tcaagctgtt gacgatgggc gacggcgctc
      661 cggtggtgcc gcgcaaccgt gaacagcgtt ttgcgatgga cgcactgctg aatgaccgga
      721 ttaagctggt gacgctgtcc ggtaaagccg ggaccggcaa aacgctgctg gccattgccg
      781 ccggttatca tcaggtgatg gataccaaag aatataaacg cctgctggtt tctcgtccga
      841 tttttccgat gggccgcgat ctgggctatc tgcccggtac gattgaggaa aagctcgatc
      901 cctggatgca gccgattcac gacgcgctgg agctggttgt tcacagccgg gcgggcgcgg
      961 cgcagaaaaa aggcggcgac ctgctggctg ccaatccgct gatcgatgtg gagccgctca
     1021 cgtatattcg cgggcgcagc attccgaacc agtttatgat tgtggatgaa gcgcagaatc
     1081 tgacgcctct ggaggtcaag acggtgatta cccgcgtcgg ccatgatacg aagattgtgc
     1141 tgaccggcga cctttaccag attgataatc cgtatgtgga cagcatgtcg aacgggttga
     1201 gcgctgtggt ggataaattc cgcggggagc cgctggctgc tcacatgctg ctgacagaag
     1261 gtgttcgttc ggaaatcgcc gaaaaggcgg ccaacctgct ttaagcgcgg atcgcttctt
     1321 tacaaaaccg gcgcggattg tttaccgttt cgtgccgtaa gaaggtcagg gaggaagacg
     1381 ggtatgatat ttcagccgag catgaaaaaa attctcgcgg cgcggggata tcatcattgc
     1441 aaaaccgatg atgaatttac gcggcacggc aaccatgccc gcaatatcca gtatgcgttt
     1501 tccaaagaat ttcaccggaa ccacgaaacg atcgtggata ttgtctttct cggggcggat
     1561 atggccggcg agaagctggt gatcggtttt ttccgtgaaa atgtatcgat caagaacggc
     1621 ataatggaaa gctccgatgt aagcattccg ctgtcgaaat tctctttggc tgagttcgag
     1681 aaacagctgg ataagctgat tcgcaagggc aatccgttcc gtaccggagt ggatggcggt
     1741 gagtcgtttt gaagaagaaa taaaggatga caggaatgat ggacgcacag aaactttttg
     1801 ccgaacgtat cggcggtgaa aactacggta aatcgaccga aatttataaa tttgaaaaaa
     1861 tcaaacgggc caaagccaaa gcccgcaagc tgcaccctga tcttgagatt ctcgacttcg
     1921 gtgtcggcga accggacgga atcgctccgg cgccgatccg tgaagcgttg aaggtggaag
     1981 tggataagcc gtcgaaccgc ggctatgccg acaacggcat tcctgaattc aagcaggccg
     2041 ccgctgaata catgaagaaa tttttcggcg tcacgctcga tccggttgcc gagatcaacc
     2101 acagcatcgg taccaagcct gcgctggcaa tgctgccgct ggcctttgtt aatcccggcg
     2161 acgtgatttt ccagactgtt cccggttatc cggtcatggc cacgcacacc agatatcttg
     2221 gcggaaaagt tgttgatatt ccgctgctgg cagaaaacgg gttccttccg gatctcaaaa
     2281 agatcgatcc ggcgctggcg gaccgctgca aacttttcta catcaactat ccgaacaatc
     2341 cgaccggcgc ggtcgccacg gatgagttct atgacgagct gatcgccttt gcgcagaaac
     2401 acaacatcct gattatccag gatgcgccgt acgccacgct ggtttatgac agcccgcgca
     2461 aatccattct ccagcgcccc ggagcgaagg actgcgcgct cgaactgcat tcgatgagca
     2521 aagcctacaa catgaccggc tggcgcctcg ccttcttctg cggcgccgcc tgggctgtcg
     2581 atgcgctggc gatgatcaaa gacaactgcg acagcggaca gtttaaagcc attcagcacg
     2641 ccgcctgcaa aggcattgcc gaccggagcc ttgcggaagc gatctgtaag cactatgagc
     2701 agcggcttcg ccgcatggtt gaagtgctgc gaggcgtcgg gtttgatgcg aaaatgcccg
     2761 ggggaacatt ctatctctac gtcaaagcgc caaaagcggc aggcagtgtg cagtttaaaa
     2821 atgccgaaga ggtatcgctg tatctgatcg aaaacttcgg catttccacc gtgccgtggg
     2881 ataacaccgg cccgttcatc cgtttcggcg cggtctttga atcgaccggc gatgcggacg
     2941 atgaacgcgt gctgagcgaa ctttccgccc gtcttaaaaa agccgatctg aagttttagt
     3001 tttcagttga agcggtgatc cggctgagaa ctttgcggga gtgttccggc aacgtgccgt
     3061 cgttataaaa agcgcggata cagtcgagcg cttcgcgggc gttctgcatc tgtttttcca
     3121 gacgctggaa agccagatga tccagttctt ccgcctgcat gatttcagcc agatgtgcgt
     3181 cgccatgcgc tttttctccg gcataggccg gaagccgccc gagaatcagc agtacgctgc
     3241 ggtgcagctc atccggctgg gccgcctgca gttcgtcgca gaaaaactca tagcagaccg
     3301 gcggacggcc tgtttcgagc gcacagcccg tttccgtcag aaatccgtac cggtcgctgt
     3361 cgagttcatc tttgccgtgt acggcacgca ggaaagggct ttccagcgct tcctcgcaca
     3421 gatcggcgcg gcagcagcag gcggtgcaga gttcgcagac ctcgcggcac tgcgcgctca
     3481 ccagttcctg cacgccgcgt tccagcgcgg catattcttc gagcagatgt ttcagttggc
     3541 tgttcacggg ggacttttcc cacgaatccc gcgaataggc acgaatatta tttaaccgca
     3601 gataactgat tttaagagga ttctgtttgc aaggcaggct ggaagcctgc gatacgtacc
     3661 gcaggcttcc agcctgcctt gctgattgat aaatctttcc aaaaac
//
LOCUS       DAOPZO010000157         3682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_33430_length_3682_cov_6.458781, whole genome shotgun sequence.
ACCESSION   DAOPZO010000157 DAOPZO010000000
VERSION     DAOPZO010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33430_length_3682_cov_6.458781"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="PKI68_08475"
     CDS             complement(<1..1250)
                     /locus_tag="PKI68_08475"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNX53764.1"
                     /translation="MTKEWISLAVFILCYVLFVFLPGKRSWVACAGGALLIAAGVLTW
                     REALFSKVSWNVMGLFFGTLILAEIFMMSRVPAVMAEWLVDKAKSTRVAMLILCALSG
                     IISMFVENVAVVLLLAPVALSLADKLKVSPVPLLIGISVSSNLQGTATMIGDPPSMIL
                     AGYMRMGFWDFFVYQGRAGIFFAVQIGAVASLLVLAWLFRQHRTKSVLLAQETARSWV
                     PASLLGLLVIGLSFATLFDPDFKWFAGTYTLILGAIALIWFRGFARWGSLRKLAKTLD
                     WDTTFFLIGIFVLVGGLSDSGWMDKLASGMSGWVGSNAALAFTVIVLFSVLVSGFVDN
                     VPFLLVMIPVAQKVADQIGAPVPLLMFGLLIGACLGGNLTPIGASANVVTLGILKKRG
                     CQVGFKEFMRIGIPFTLAAVLSACA"
     gene            complement(1378..2283)
                     /locus_tag="PKI68_08480"
     CDS             complement(1378..2283)
                     /locus_tag="PKI68_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNX53765.1"
                     /translation="MEVSRKGLVNFAWMSTAAAVLTIGLKTAAYFLTGSVGLLSDALE
                     SLVNLAGGLMAVAMLTVAARPADEEHPYGHSKAEYFSSVVEGALILLAAASIGAAAVH
                     RLIFPRMLEQIGLGLGISVAASLVNLAVAIVLLRASRRHDCITLKANAHHLLTDVWTS
                     AGVLVGIGAVALTGWQRLDPIIALIVAGNIVWTGVRIMRDSVGGLMDAAISAEDQQKV
                     RSILEAHLEEGAQYHALRTRQAGAQRFISFHMLVPGDWTVARGHQLIDHIETDLRCAL
                     SHVTVFSHLESLEDPASWDDTSPGC"
     gene            2499..3314
                     /locus_tag="PKI68_08485"
     CDS             2499..3314
                     /locus_tag="PKI68_08485"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNX53766.1"
                     /translation="MTVLLFLKTLLLGLIEGITEFLPVSSTGHMILADEFIRLSENRQ
                     FTAVFEVFIQSGAILAVVVLFRRELWPFAGTGPERRNKWLLWAKTFVAFIPAAVAGLF
                     FHRFIKEYLFNPVTVAATLIFYGVILILFEKKHRVKPDALHCIDGLTFRIAFLIGIIQ
                     CLALIPGTSRSTATILGGMILGLDRTLAAEFSFFLAIPTIAAASLFQLLESGVAFSMT
                     EYLVLGIGFAVSFLVAAGVIEWLMHYLKKHSFIPFGWYRILLGLIVLGWWYTK"
     gene            complement(3324..>3682)
                     /locus_tag="PKI68_08490"
     CDS             complement(3324..>3682)
                     /locus_tag="PKI68_08490"
                     /inference="COORDINATES: protein motif:HMM:NF037628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53767.1"
                     /translation="LQSADLRPVLEIDRAVTLGEADASLVDGLKRTGPFGQDNPEPVW
                     AVCGVKAVDSRILKEKHLKLTLSDGKARCEAIGFNLAEKLPSGPIDVAFTLQENVWNG
                     RTTLQLNIRDIRPAAK"
BASE COUNT          894 a         1131 c          949 g          708 t
ORIGIN      
        1 gcgcaggcgg aaagaaccgc cgcaagggtg aagggaattc caatccgcat aaattctttg
       61 aatccgacct gacagccgcg cttcttcaag attccgagcg tgacgacatt ggcggatgcg
      121 ccgatgggcg tcaggttgcc gccgagacag gcgccgatca gcaggccgaa catgagcagc
      181 ggaacgggcg cgccgatctg gtcggccact ttctgggcga ctggaatcat caccagcaga
      241 aacggcacgt tatctacaaa tccggacacc agaaccgaaa acagcacgat gacagtgaaa
      301 gcgagcgcgg cgttggagcc gacccagccg gacatgccgg aggccagttt atccatccat
      361 ccggaatcac tgaggccgcc gaccagcacg aagataccga tcaggaaaaa agtcgtatcc
      421 cagtcaagtg tcttggccag tttacgcaaa cttccccagc gcgcaaatcc gcggaaccag
      481 atcaacgcaa tcgcaccaag aatcaaggtg taggttccgg caaaccactt aaagtccgga
      541 tcgaacagcg tggcaaaaga gagtccgata accagcagac ccagcagcga cgccggaacc
      601 catgaacggg cagtttcctg agccagcaaa accgatttgg tgcggtgctg acggaacagc
      661 caggccagca ccagcagtga ggcgaccgca ccgatttgaa cagcgaagaa aataccggcc
      721 cgcccttgat acacaaagaa atcccagaag cccatgcgca tataaccggc cagaatcatg
      781 ctcggcggat cgccgatcat cgtcgccgtt ccttgcaggt tgctgctgac cgaaataccg
      841 atcagcagcg gcacagggct gactttgagc ttgtcggcca gactcagcgc aaccggtgca
      901 agcagcagca ccacggcaac attttccacg aacatcgaaa tgatgcccga cagcgcacac
      961 aggatcagca tagcaacacg cgtgcttttg gctttatcca ccagccattc ggccataacc
     1021 gccggaacgc gcgacatcat aaaaatctcg gccagaatca gggtgccgaa aaaaagcccc
     1081 atcacgttcc agctgacttt actgaaaagg gcttcccgcc atgtcagcac accggcagcg
     1141 atcagaagcg cgccgccggc gcatgcgacc caggaacgtt tcccgggcag aaagacgaac
     1201 agaacatagc aaaggataaa tacggctaaa ctgatccatt ctttcgtcat aaacggcttc
     1261 cggtttcatg tgattgaccg cgcggttata ccggcaggcc gacatgttgt aaaccctatt
     1321 cggaccgcgc cggtattgcg taaaacagat ttacaaagtc agcagagaac cgggatttca
     1381 gcagcccgga cttgtatcat cccaggaggc gggatcttcc agcgattcca gatggctgaa
     1441 tacagtgacg tgcgacaaag cacagcgcag atcggtctca atatgatcaa tcagctgatg
     1501 cccgcgagcc accgtccagt cccccggaac cagcatatgg aaagagataa accgctgagc
     1561 gccggcctgc cgggtgcgca acgcatgata ttgcgcgccc tcttccaaat gggcctccag
     1621 aatactccgg actttctgct gatcttcggc ggatatcgcc gcatccatga gtccgcccac
     1681 cgaatcccgc ataatgcgga caccggtcca gacaatgtta ccggcaacga tcagtgcaat
     1741 aatcggatcc agacgctgcc agccggtcaa cgccacagca ccgataccga ccagaacgcc
     1801 ggccgaggtc cacacgtctg tcagcaggtg atgcgcattc gccttgagtg taatgcaatc
     1861 gtgacggcgg ctggccctga gaagaacaat cgcaaccgcc agattcacca gcgaggccgc
     1921 aacggagatc cccagcccca gcccgatttg ttcaagcatt cttgggaaaa taagccggtg
     1981 aacggcggcc gcaccgatgc tggcggccgc cagcagaatt aaggctcctt cgacgacact
     2041 tgaaaaatac tcggccttgc tgtgcccata cggatgctct tcatccgccg ggcgggcagc
     2101 gaccgtcagc atggccacag ccatcagtcc accggccaga ttgacaagcg actcaagggc
     2161 gtcggaaagc agcccgaccg aaccggtgag aaaatacgca gcggttttca aaccgatcgt
     2221 aagaaccgcc gcagccgtag acatccaggc aaaattaaca agacccttgc gcgatacttc
     2281 catataaatt tttcccggta caaaacttaa gccgaaacac atcgtctgaa tacgatttgg
     2341 ccgtcgcgat agccagaaaa aacgcctgcc cgccggcagt ttccgcccgg atgctcaccg
     2401 gcctccggct ttccctaaaa cagctttgat ttgtcgttct ccgcctgtca tagtgcgcgc
     2461 gttttatttt gccacataaa aacctgtctg gggccccgat gactgttctg ctttttttaa
     2521 aaacacttct gctgggactg attgaaggaa tcaccgaatt tcttcccgtc agcagcacgg
     2581 gacacatgat tctggccgac gaattcatcc ggctttccga aaaccggcag ttcacggccg
     2641 tcttcgaagt cttcattcag tccggcgcga ttctggcggt cgtcgtactc ttccgccgcg
     2701 aactgtggcc ctttgccgga accggaccgg aacggcgcaa caaatggctt ctgtgggcaa
     2761 aaacgttcgt cgccttcatc cccgccgctg tcgccggact gttcttccac aggttcatta
     2821 aggaatacct ttttaacccc gtcaccgtcg cggcaacact gattttctac ggcgtcattc
     2881 tcatcctgtt cgaaaaaaaa cacagagtaa aaccggatgc actgcactgc attgacggct
     2941 taaccttccg catcgccttt ctgatcggga tcatacagtg ccttgcgctg attcccggaa
     3001 cctcgcgatc aaccgccacc atcctcggcg gaatgattct tggactggac cggacgctgg
     3061 cggcggaatt ctccttcttt ctggcgatcc caaccatcgc tgccgccagc ctgtttcagc
     3121 tgctcgaaag cggcgtcgcc ttctcaatga ctgaatacct cgtactcggc ataggattcg
     3181 ccgtctcgtt tctcgtagct gccggcgtca tcgaatggct gatgcactac ctgaagaaac
     3241 acagcttcat cccgttcggc tggtaccgga tcctcctcgg actgatcgtt ctcggctggt
     3301 ggtacacgaa gtaaacctga cggtcacttt gccgcggggc ggatatcgcg gatattgagc
     3361 tgcaaggtgg tgcggccgtt ccagacattt tcctgcaagg taaaggccac atcaatcggg
     3421 ccggacggca gcttttccgc cagattaaac ccgatcgcct cgcagcgcgc cttcccgtcg
     3481 gaaagcgtca gtttgagatg cttttcctta aggatacggc tgtcaaccgc cttcactccg
     3541 cacaccgccc agaccggctc cggattatcc tgtccgaacg gaccggtccg cttgagtccg
     3601 tccaccagtg atgcatccgc ttcaccaagc gtaacggcgc ggtcgatttc cagtacgggc
     3661 cggagatcgg cggactgaag ct
//
LOCUS       DAOPZO010000158         3623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_34256_length_3623_cov_9.935258, whole genome shotgun sequence.
ACCESSION   DAOPZO010000158 DAOPZO010000000
VERSION     DAOPZO010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34256_length_3623_cov_9.935258"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /gene="larE"
                     /locus_tag="PKI68_08495"
     CDS             <1..651
                     /gene="larE"
                     /locus_tag="PKI68_08495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HNX53768.1"
                     /translation="TLTDDEKAEAVQLAEKIGAKHLLIETDEFDDALFTANTAERCYF
                     CKKVRFQALAAWAAARGYPWIVEGSNVDDDRDYRPGSRAVAELPTVRSPLKEAGLTKA
                     EIREISKQWDLPTWEKPSAACLASRIEYGLPLTPERLKQVEEAEKIIRPLCKGQLRVR
                     HHGTLARIEVEPEWISKLAEPETARKISEKLKALGFHHVALDLSGYRMGSLNEDLK"
     gene            648..1316
                     /gene="larB"
                     /locus_tag="PKI68_08500"
     CDS             648..1316
                     /gene="larB"
                     /locus_tag="PKI68_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HNX53769.1"
                     /translation="MKLFEEMGFAKIDHHRADRCGAPEAIFCQGKTPEQVAQIVERMN
                     QGAHNILATRANAEMFAAVKKLVPEAEYHEAAKLIIVRKEALTPDPDRFILVMTAGTS
                     DIPVAEEAALTAELMGHRVERVFDVGVAGIHRLLAQEEKIRGANVIIVAAGMEGALAS
                     VVGGLVSKPVIALPTSVGYGASFEGLAALLGMLNSCAAGIAVVNIDNGFGAGRLAGMI
                     NAMR"
     gene            1346..2584
                     /gene="larC"
                     /locus_tag="PKI68_08505"
     CDS             1346..2584
                     /gene="larC"
                     /locus_tag="PKI68_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HNX53770.1"
                     /translation="MKKTTLSNTHTPAHLDTLYLSGFSGVSGNMMIGALLDAGLPEEA
                     FRRMVSALPVSGYQLKVEKVMKRGISATHFDVELDHSEQHTHRQLADIIKIIEAAELS
                     AAVKARSIAVFTRLAEAEAKVHGTTVEKIHFHEVGAVDAIIDIVGTVFGLAALGIEKV
                     YAGNLRTGFGVIQCAHGSMPIPAPATAELLNGIPYTFGDIEKELLTPTGAALLAVLCD
                     GFGDRPDGFVSEKTAYGAGGRELDIPNVLRAEVGRVRRTRRGQLGDLSLPEKLLVLET
                     NIDDCNPQIFDYVMERLFKAGALDVWLTPIQMKKNRPAVMLSVLAPAMIQGAVEQILF
                     SETTTIGIRHYPVQRTAADRREETVATPWGLVRVKISSINGKTISATPEYDDCRKLAE
                     ASGVPLKEIIALAGNQNVDF"
     gene            2611..2805
                     /locus_tag="PKI68_08510"
     CDS             2611..2805
                     /locus_tag="PKI68_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53771.1"
                     /translation="MNAQPPKKRLWRRDGPVPNPYRDTLSQEIGYKLDSRYAGCLLVV
                     FAMALLLVSGFAFLVYWLMH"
     gene            complement(2808..>3623)
                     /locus_tag="PKI68_08515"
     CDS             complement(2808..>3623)
                     /locus_tag="PKI68_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNX53772.1"
                     /translation="KETERDVALRVEETGGDAFIVSGRGVLHLSILIETMRREGFELT
                     IAQPRVIFRTHHGIKEEPIEILTVDVPDEYAGKAIELIGQRRGEMIRMDQLGLRKNMT
                     FHIPTRGLIGLRSKMMTATAGEGIINHRFLHYAPYAGEINHRNNGVLISMGNGTSVAF
                     AIDGLQQRGIFFIDPGVECYEGMIVGEHCLDKDLLVNVQKAKQLTNMRASGSDRAMKI
                     APAQKLSLEEALEYIKDDELVEVTPHNVRLRKRYLTDNERKRMKRSAAEEETA"
BASE COUNT          761 a         1017 c         1105 g          740 t
ORIGIN      
        1 acgctgaccg atgacgaaaa agccgaggcc gtccagctgg cggaaaaaat cggcgccaaa
       61 cacctattga tcgaaaccga cgaattcgac gacgcgctgt ttaccgccaa caccgccgaa
      121 cgctgctact tctgcaaaaa agtccgcttc caggcgctgg ccgcatgggc cgccgcgcgc
      181 ggctatccgt ggattgtcga aggcagcaat gtcgatgacg accgcgacta ccggcccggc
      241 agccgcgccg tcgcggagct gcccaccgtg cgcagcccgc tcaaagaagc cggactgacc
      301 aaagcggaaa tccgcgaaat ttcgaaacag tgggatctgc cgacgtggga aaagccgagc
      361 gccgcctgcc tcgcctcgcg catcgaatac ggactgccgc tgacacccga acggctcaag
      421 caggtcgaag aggccgaaaa aatcatccgc ccgctttgca aaggacagct gcgcgttcgg
      481 catcacggca cactggcgcg catcgaagtc gaaccggaat ggatttcaaa gttggcggaa
      541 ccggaaaccg cgcggaaaat ttcggaaaag ctgaaagctt tgggctttca ccacgtcgcg
      601 ctggatcttt ccggctaccg gatgggaagc ttgaacgagg atttgaaatg aagctgtttg
      661 aagaaatggg attcgcgaag atagatcatc atcgggcgga tcgttgcggc gcgccggaag
      721 cgattttctg ccagggcaaa acgccggagc aggtggcgca gattgtcgag cggatgaatc
      781 agggcgcgca caatattctg gcgacgcggg cgaacgccga aatgttcgcg gcggttaaaa
      841 agctggttcc ggaagcggaa tatcacgagg cggcgaagct gattatcgtg cgcaaggaag
      901 cgcttacacc tgaccccgac cgctttattc tggtgatgac ggccggcacg agcgatatcc
      961 ccgtggccga agaggccgcg ctgacggcgg agctgatggg ccatcgtgtc gaacgtgttt
     1021 ttgacgtcgg cgtggccggc attcaccggc tgttagcgca ggaagaaaaa atccgcggcg
     1081 cgaatgtgat catcgtcgcc gccgggatgg aaggcgcgct cgccagcgtg gtcggcggac
     1141 tggtttcaaa accggtgatc gcgctgccga cgagcgtcgg ctacggcgca agcttcgaag
     1201 gactggccgc cctgctcggt atgctcaaca gctgcgccgc cgggattgcc gtggtgaata
     1261 ttgataacgg gttcggtgcg ggacggctcg ccgggatgat caacgctatg cgttgaggga
     1321 gtgtgtgcgt gacggagtaa agaatatgaa aaaaacaacg ttgtctaata ctcacacacc
     1381 cgcacacctc gacactcttt atctctccgg cttctccggc gtcagcggaa acatgatgat
     1441 cggcgcgctg ctggatgcgg ggctgccgga agaagcgttc cgccgcatgg tgagcgccct
     1501 gcccgtttcg ggctatcagc tgaaagtcga aaaagtcatg aagcgcggca ttagcgcaac
     1561 gcattttgat gttgagctgg atcattcgga acagcacacg caccggcagc ttgccgacat
     1621 cattaaaatt atcgaagcgg ctgaactcag cgcggcggtg aaggcgcgca gcatcgccgt
     1681 ctttacgcgg ctcgccgagg ccgaagccaa agttcacggc acgacggtgg agaagattca
     1741 ttttcacgaa gtcggcgcgg tggacgccat tatcgatatt gtcggtacag ttttcgggct
     1801 cgcagcgctc ggcatcgaaa aagtgtacgc cgggaatctg cggaccggct tcggcgtgat
     1861 tcaatgcgcg cacggttcca tgccgattcc cgcgccggct acggcggagt tactgaacgg
     1921 tatcccgtac accttcggcg atattgaaaa ggagctgctg actccgaccg gcgccgcgct
     1981 gctcgccgta ctctgcgatg gtttcggcga ccggcctgac gggtttgttt cagaaaaaac
     2041 cgcctatggc gcgggcggcc gggagctcga cattccaaat gttctgcgtg ccgaggtggg
     2101 gcgcgttcgc cgaacgcgcc gcggacagct cggcgatctg tccctacctg aaaaactgct
     2161 ggtgctggaa accaatatcg acgattgcaa cccgcagatt ttcgattatg tgatggagcg
     2221 gctttttaaa gccggtgcgc tggatgtctg gctgacgccg atccagatga aaaaaaaccg
     2281 tccggctgta atgctttcgg ttcttgcgcc ggccatgatt cagggcgccg tcgaacagat
     2341 tcttttcagc gaaaccacca ccatcggcat tcgccattat ccggtacagc gtaccgccgc
     2401 cgaccgccgc gaagagacgg tcgcgacgcc gtggggattg gtgcgcgtaa agatcagttc
     2461 gatcaacggc aaaaccatat cggcgacgcc ggaatatgac gactgccgga aactggctga
     2521 agcgagcggc gtacctctta aagaaattat agctcttgcc ggaaatcaaa acgttgattt
     2581 ctgaaaggga gtcccctaca ttccgcaccc atgaacgcgc agccgcctaa aaaacgactc
     2641 tggcgtcggg atggtccggt tcccaacccc taccgcgata cactgagcca ggaaatcggt
     2701 tataaactgg actcccgcta tgcgggatgc ctgctggttg tttttgcaat ggccctgctg
     2761 ctggtgtccg gcttcgcttt tctcgtctac tggctcatgc actgagccta agccgtttct
     2821 tcctcggcgg cggaacgttt catgcgtttg cgttcgttat cggtgaggta gcgtttgcgc
     2881 aggcggacgt tgtgcggcgt cacttcaacc agctcgtcgt ctttgatgta ttccagcgct
     2941 tcttcgaggc tgagtttctg cgccggtgca attttcatgg cgcggtccga tccggaagcg
     3001 cgcatgttag tgagctgctt ggctttctgc acattaacga gcagatcttt atcaagacaa
     3061 tgctcgccga cgatcatgcc ttcgtaacat tcgacgcccg gatcgatgaa gaagatgccg
     3121 cgctgctgca ggccgtcgat ggcgaatgcg acggaggtcc cgttgcccat gctgatcaag
     3181 acgccgttgt tgcggtggtt gatttccccg gcatagggcg cgtaatgcag gaagcggtgg
     3241 ttgatgatgc cttcgccggc cgtggcggtc atcattttgc tgcgcaagcc gatcaatccg
     3301 cgggtgggaa tgtggaaggt catgtttttg cgcagaccaa gctgatccat gcggatcatt
     3361 tcgccgcgcc gctggccgat cagttcgatg gctttgccgg cgtattcgtc cggaacgtcc
     3421 accgtcagaa tttcaatcgg ctcttctttg atgccgtgat gcgtgcggaa aatcacgcgc
     3481 ggctgagcga tcgtcagctc aaacccttcg cggcgcatgg tttcgatcag aatggacagg
     3541 tgcaatacgc cgcgtccgct gacgatgaag gcgtcgccgc cggtttcttc aacgcgcagc
     3601 gccacgtcgc gctcggtttc ttt
//
LOCUS       DAOPZO010000159         3612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_34405_length_3612_cov_10.699747, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000159 DAOPZO010000000
VERSION     DAOPZO010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34405_length_3612_cov_10.699747"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PKI68_08520"
     CDS             complement(<1..826)
                     /locus_tag="PKI68_08520"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNX53773.1"
                     /translation="MNIGFSAFVMQGGRSGVASYIRELIGHLQQEDPVSHYDIMMPMR
                     EAELIKLTAPNFQKSLYSDFISRPVVNIAWHNTMLPVIGKIRKFDLLHIPSYRRIPLV
                     KKCPLVATVHDVATLSMDAKYDAARMFYNRRIVPSQIKNADHIITVSHYSKKDIVERV
                     GVAPEKITVLYSGINHQIFRPVEKAAARAQLAERYKLDRPFIIYVSRLEHPAKNHVRL
                     IEAFERFKAESDSPHKLVLPGADWNGADIIRARAATSPVHDDILFPGFVPLEDMPLF"
     gene            935..2059
                     /locus_tag="PKI68_08525"
     CDS             935..2059
                     /locus_tag="PKI68_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53774.1"
                     /translation="MKRKNIFRILIAVFLCGIAQGAEPVQIDFFFEPGCRECEQIQSE
                     LLPGIEKKFPGACRIQRHDIGVETNFINLLQMENRLGYEAPDRAYLIVNKQYIFGAEP
                     SHEEFAALISNLLTQESAGPAPAAASTALAQKWYEGFTVPAVAAAGLLDGMNPCAIST
                     LVFFMSLLAVAKVRNRQLILLGIAFCFASFLTYLALGFGLFRVLHLFSGFTVLRSVVE
                     NAMAVILLVLAALSVRDAVRFRKTGQSADVTLQLSTGLKKRIHDVMRRGLGSASIFWG
                     GLLIGTAVTVLESVCTGQVYVPTLVLILKDSTYSESRAWLLLLLYNLLFILPLVTVFT
                     AVYFGLKTEALLTWSRRNVVASKLLLGLFFVLMALFIFWR"
     gene            complement(2233..3600)
                     /locus_tag="PKI68_08530"
     CDS             complement(2233..3600)
                     /locus_tag="PKI68_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HNX53775.1"
                     /translation="MVKILCIGAGYVGGPTMAMIAKKNPKVKVTVVDINQKRIDAWNS
                     DTLPIYEPGLDEVVKAARGKNLFFSTNVVKGIKEADIIFVSVNTPTKMFGHGAGCAAD
                     LQYWEKCARQILEVSTSDKIVVEKSTLPVRTAAAMERILHSGKTGVHFDVLSNPEFLA
                     EGTAIADLENPDRILIGGHPTPRGKKAVKALADVYAAWVPKEKILTTNLWSSELSKLT
                     ANAFLAQRVSSINSISALCEATDADVGEVARAIGTDSRIGPKFLKASVGFGGSCFKKD
                     ILNLVYLCETYNLHEVAEYWRQVVKMNEYQERRFVARMLKEMFNTIAGKHIALFGFAF
                     KADTGDTRESPAILIARELLAENAKIVITDPQAMEHAKVDLADVIDRITFEPDPYKAA
                     KGAHAVALLTEWKEYKTLDYQKIFDGMIQPAFLFDGRNHLDHQALHKIGFNVYSIGKA
                     ALKHI"
BASE COUNT          717 a          991 c         1052 g          852 t
ORIGIN      
        1 agaaaagcgg catatcttcc agcggcacga atccggggaa aagaatgtcg tcatgaaccg
       61 gactggtggc ggcgcgcgcc cggattatat ccgcgccgtt ccagtccgcg cccggcagaa
      121 ccagtttatg cgggctgtcc gattcggcct tgaaccgctc gaacgcctcg atcagccgga
      181 catggttttt cgccggatgt tccagccgcg aaacatagat gatgaagggc cggtcgagct
      241 tgtatcgttc ggcaagctgg gcgcgggcgg cggctttttc aacggggcgg aaaatctgat
      301 ggttgatgcc ggagtagagc actgtgattt tttccggcgc tacgccgaca cgttcgacga
      361 tatctttttt cgaatagtgg ctgaccgtga tgatgtggtc ggcgtttttg atctgcgacg
      421 gaacgatgcg gcggttatag aacattcgtg cggcgtcata cttggcatcc atcgaaagag
      481 ttgcgacgtc atgcaccgtc gcgacaagcg ggcatttttt caccagcgga atccgccggt
      541 agctcggtat gtgcagcaga tcaaatttcc ggatttttcc gataaccgga agcatcgtat
      601 tgtgccacgc gatgttaacc accggacggg aaatgaagtc cgagtagagc gacttttgaa
      661 agttcggtgc ggtcagcttg atcagctcgg cctcgcgcat cggcatcatg atatcgtaat
      721 ggctgaccgg atcttcctgc tggaggtggc cgatcagctc gcggatgtag gacgccacgc
      781 cggagcgccc gccctgcatc acaaaagctg aaaatccaat gttcattggc gacggattct
      841 aaacacaccg gcgcgctttt aaaataaact tccctgtcaa aattggcgca cagccttggc
      901 aggggttcgg atgccggtgt acaatgtcat aacaatgaaa aggaagaaca ttttcagaat
      961 tctgattgct gtattccttt gcggaatcgc gcagggggcg gagccggtgc agatcgactt
     1021 tttcttcgag cccggctgcc gtgagtgcga gcagatacag tcggagcttc ttccggggat
     1081 tgaaaaaaaa ttccccggcg cctgccggat tcagcggcat gacatcggcg tagagaccaa
     1141 cttcatcaac ctgcttcaaa tggaaaaccg gctgggctat gaggcgccgg atcgggccta
     1201 tctgatcgtc aacaaacagt acattttcgg cgcggaaccg tcgcacgaag aatttgcggc
     1261 gctgatttca aatctgctga cgcaggagtc tgccggcccc gcgccggcgg cagcttcaac
     1321 cgctctggcg cagaaatggt atgaaggctt tacggttccg gccgttgccg cggcgggact
     1381 gctggacgga atgaatccct gcgcgatttc aacgctggtc ttctttatga gtctgctggc
     1441 ggttgccaag gtccggaacc gtcagctcat cctgctcggc atcgcctttt gtttcgcttc
     1501 gttcctgacc tatctggcgc tgggcttcgg attgtttcgc gtcctgcacc tgttctcagg
     1561 ttttaccgtt ctgcgcagcg tggtggagaa cgccatggcg gtcattctgc tggtgctggc
     1621 cgcgctgtcg gtgcgcgacg ccgttcgttt ccggaaaacc ggacagtccg ctgatgtcac
     1681 gcttcagctt tccaccggcc tgaaaaagcg gattcatgat gtcatgcgcc gcggcctcgg
     1741 cagcgccagt attttctggg gcggactgct gatcggaacc gccgtcacgg tgctggaaag
     1801 cgtctgcacc ggacaggttt atgttccgac gctggttctg attctgaagg acagcaccta
     1861 ttcagaatcg cgggcgtggc tgcttctgct gctctacaac cttctgttta ttctgccgct
     1921 ggtaacggtc tttacggcgg tttacttcgg cctaaaaacc gaagcgctgc tgacgtggag
     1981 ccgccggaac gtggtcgcct cgaaactgct cctcgggctt ttctttgtcc tgatggcgct
     2041 gttcatcttt tggagataag ccgcgtcgtt gtaggacagg cttctctgta ttcagagagc
     2101 ctgtgttcgc cgaagaggca ttaaaagtac acgctcccgg atacgggact cgaccaacgg
     2161 gagagaggct tcgttgttgc gaagcaacga ccaacgggag tgtcaaccgc gtcctacaat
     2221 acggaaaaca ggttagatgt gcttcagcgc ggccttgccg atcgagtaga cattgaagcc
     2281 gatcttgtgc agcgcctgat ggtcgagatg gttgcgtccg tcgaacagga aagcgggctg
     2341 gatcatgccg tcgaaaatct tctggtagtc gagcgtcttg tattccttcc actcggtcag
     2401 cagggcgacg gcgtgcgcgc ctttggccgc cttgtaagga tccggctcaa acgtaatgcg
     2461 gtcaatcaca tcggccagat caaccttggc gtgttccatt gcctgaggat cggtgatcac
     2521 tatttttgca ttttccgcca gcagttcgcg ggcgatcaga atggccgggc tttcgcgcgt
     2581 atcgccggta tcggctttaa aggcgaagcc gaacagcgcg atatgcttgc cggcgatcgt
     2641 gttgaacatc tccttcagca tgcgggcgac gaaacggcgc tcctgatatt cgttcatctt
     2701 caccacctgc cgccagtatt cggccacttc gtgcaggttg taggtttcgc agaggtacac
     2761 gagattcaga atatccttct tgaagcacga gccgccgaag ccgacgctgg ccttcagaaa
     2821 tttcgggccg atccggctgt cggtaccgat cgcgcgcgcc acttcgccga catcggcgtc
     2881 ggtcgcttcg cacagcgcgg agatgctgtt gatcgaactg acgcgctggg cgaggaacgc
     2941 attggccgtc agcttggaaa gctcgctcga ccagagattg gtcgtcagga ttttttcctt
     3001 cggcacccac gcggcgtaaa catccgccag cgccttaacg gcttttttac ctctgggcgt
     3061 cggatgcccg ccgatcagaa tacggtcggg attttccagg tcggcaatcg ccgtgccttc
     3121 ggcgagaaat tccggattgg aaagaacgtc gaaatgtacg ccggttttgc cgctgtgcag
     3181 gatgcgttcc atggcggcgg cggtgcgaac gggcagggtg ctcttttcaa ccacgatctt
     3241 atcagaggtc gaaacttcca gaatctggcg cgcacacttt tcccagtact gcaggtcggc
     3301 ggcacagccc gcgccgtgac cgaacatctt ggtcggcgtg ttcacgctca cgaaaataat
     3361 gtcggcttcc ttgatgcctt tgaccacgtt ggtggagaag aaaaggtttt tgccgcgtgc
     3421 cgccttgacc acttcgtcca gacccggctc gtagatcggc agagtatcgg aattccaggc
     3481 atcgatgcgc ttctggttga tgtccaccac ggtcactttg accttcggat tctttttggc
     3541 gatcatcgcc atcgtcggcc cgccgacata acctgcaccg atgcaaagaa ttttgaccat
     3601 aatcaactcc ta
//
LOCUS       DAOPZO010000160         3599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_34596_length_3599_cov_8.387980, whole genome shotgun sequence.
ACCESSION   DAOPZO010000160 DAOPZO010000000
VERSION     DAOPZO010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34596_length_3599_cov_8.387980"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PKI68_08535"
     CDS             <1..225
                     /locus_tag="PKI68_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53776.1"
                     /translation="PTYPGPYDFTESVVVNEIIPMIDEIARQTGATVIDLHRPLSGKA
                     GLFPDTVHPNAEGARLMAEAVFAAIAAEKK"
     gene            448..3006
                     /locus_tag="PKI68_08540"
     CDS             448..3006
                     /locus_tag="PKI68_08540"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNX53777.1"
                     /translation="MKIRMCVLGVLLALPVSIFAAESSMTFSNQWLKAGFTQQGGTWR
                     LSSLSRLNGKDALAIADDGFEVLRFNGNRYTLDDYAVKDVAAKTDGDIQSIIFTCVPA
                     KSGTDAPAEVALEYQLDKNPELHKTVRLKLAEGDRIDRLEVWRLSTDSKASRGGDNGQ
                     PVFVGDWFIGLDYPFFYSRHSDGFKQPAFHYIYHYSINLEGGDREYAPRNGLITVFHF
                     PNKAKAQPGNGWGIVSKRGVIGLPAKPNDGAELALFDYIERTRPRSRSYLHFNNWYTP
                     EAKQLTVDGFGTQLTKSIKEPLARYGARLDAMVPDDGWQEKNFTRIYEQSKQHEPLDK
                     VAEAVRKQGVDFGIWVGMFGRGVNAKLAEKAGYRPAYPLDMPPLALSHKATFYDLTDE
                     NYQRDYKESMRWLIQNCGVRYIKHDFNYMFHTYAALRHAREEAADATLDMIAYERSLA
                     PGLLINFTNGSMFTPFLLQHAEYMWMNSGDSGSNFVFPLLSKLEVATSYRDHHFYKSW
                     GDPTNTVRPLIPIANFMTHGVLHAGKKIYFEKGDTLQDWCNYAVMYVGRGTLLKELYI
                     SPSLFTEPMWEALGKACAWSQANTDLLRNSWFIGGDCDQGEPYGYISWNGNRALLTVR
                     NPDRLEKPLTVPFDRTVYYRGETGKPFRAKAIYPFTEQMPWKLTSGQPFTLQIPGDSV
                     MVFELEAGLPQSTGLVAPSPLPAFSANTNGKSFEITLTVPDEEALRYDLVVLTESAKP
                     AKPASRKDKTPMDRAAITVNNAAITVNNAAVEPQRDRAGTGFVISSFDLRPYRGQQIV
                     IKGGGTNNLTLRPAVWLVCDRPVKEPAPPENRILPMQISRGYRHQSQLLIAPEK"
     gene            3117..3329
                     /locus_tag="PKI68_08545"
     CDS             3117..3329
                     /locus_tag="PKI68_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53778.1"
                     /translation="MKNKPLILEELIIWLRGRFTMTDEEKTCMLLILVICWVGLVSRY
                     FYLKNQAPPAAEKLIEDGDVSQKETK"
BASE COUNT          817 a         1044 c          983 g          755 t
ORIGIN      
        1 ccgacctatc ccggcccgta tgatttcacc gagtcggttg tcgttaacga aattattccg
       61 atgatcgacg agatcgcgcg gcagaccggc gcaacggtta ttgatctgca ccgtccgctg
      121 agcggcaaag ccgggctgtt tccggacacg gtgcatccga acgccgaagg tgcgcggctg
      181 atggccgaag cggtcttcgc cgccattgcg gccgagaaaa agtgaagaat tcagatccga
      241 acgattccgg tgccgccggc cgtaaaacgc agccgggctg caaccccgcc cgaatttaat
      301 ttgtcaaaat ctggcggttg gtctatctcg ctgatagact gcgaaacgtt ttagccggag
      361 acatcagccc cgcggtgtct tgtccgattc gactttcgcc actgttgaaa aagcgcccgg
      421 gagccgggca ataagaaagg tgtaccgatg aagataagga tgtgcgtttt gggagttctg
      481 ctggctctgc cggtttctat tttcgcggcc gaaagcagta tgacgttcag caaccagtgg
      541 ctgaaggccg gctttacaca acagggcggc acctggcggc tgagcagcct gtcccgtctg
      601 aacggcaagg atgcgctggc cattgcggat gacgggttcg aagttctgcg attcaacggc
      661 aaccgctata cgctggatga ttatgccgtg aaggatgtgg ccgccaaaac ggacggcgat
      721 attcaatcga tcatcttcac ctgcgttccg gccaagagcg gtaccgatgc gcctgctgag
      781 gttgcgctgg aatatcagtt ggataagaat ccggaactgc ataaaaccgt ccgcctgaag
      841 ctggcagaag gcgaccgcat tgaccgcctc gaggtctggc ggttatccac ggattccaag
      901 gcttcgcgcg gcggcgacaa tggtcagccg gtattcgtcg gcgattggtt tatcggtctg
      961 gattatccgt tcttctacag ccgccacagc gacggcttta aacagcccgc ctttcattac
     1021 atatatcact actcgatcaa tctggaaggc ggcgatcggg agtatgcgcc gcgcaacggt
     1081 ctgattacgg tttttcattt cccgaacaag gccaaagcgc agcccggcaa cggctggggc
     1141 atcgtcagca agcgcggtgt gatcgggctg ccggccaaac cgaacgacgg cgccgagctg
     1201 gcgctgtttg attacattga acgcacgcgg ccccgttccc gcagctatct gcatttcaat
     1261 aactggtaca cgccggaagc caagcagctg acggtggacg ggttcggtac gcagctgacg
     1321 aaaagcataa aagagccgct ggcccgttac ggcgccagac tggatgcaat ggttccggac
     1381 gatggctggc aggagaaaaa cttcacccgg atttacgaac agtcgaagca gcacgagccg
     1441 ctcgacaaag tggcggaagc tgtccgcaag caaggggtgg atttcggcat ctgggttggc
     1501 atgttcggcc gcggcgtcaa cgcaaagctt gcggagaaag cgggttatcg cccggcctac
     1561 cccctggaca tgcctccgct ggcactaagc cataaggcga ctttctatga tttaaccgac
     1621 gagaattacc agcgggacta caaagagtcc atgcgctggc tcattcagaa ctgcggggtc
     1681 cgctacatca agcacgactt caactatatg ttccacacat acgccgcact tcggcatgcc
     1741 cgggaagagg ctgcagatgc aacgctggat atgattgcgt atgagcggtc gctcgcaccg
     1801 ggcctgctga tcaactttac caacggctcc atgttcacgc cgttcctgct ccagcatgcc
     1861 gaatatatgt ggatgaacag cggcgactcc ggcagcaact ttgtctttcc gctgctctcc
     1921 aaactggagg tggccacttc gtatcgcgac catcattttt ataaaagctg gggcgacccg
     1981 accaacaccg tccgcccgtt gattccgatc gctaacttca tgacccacgg ggtgcttcac
     2041 gccggcaaaa aaatctactt cgaaaagggc gacacgctgc aggactggtg caattacgcg
     2101 gttatgtatg tcggccgcgg cacgttgctg aaggaacttt atatttcacc cagcctgttc
     2161 accgagccga tgtgggaagc cctgggtaaa gcctgcgcct ggtcacaggc caacaccgat
     2221 ctgctgcgca acagctggtt catcggcggc gactgcgatc aaggtgaacc ctacggctac
     2281 atcagctgga acggaaaccg cgccctcctg accgtccgca atcccgaccg gctggaaaaa
     2341 ccgctgaccg ttccattcga ccgaaccgtt tactatcgcg gcgaaacagg caaacccttc
     2401 cgcgccaaag ccatctaccc gttcaccgaa cagatgccgt ggaaactgac ttccggacag
     2461 ccgttcaccc ttcaaatccc cggtgactct gtgatggtct tcgagctgga ggccggcctg
     2521 ccgcaaagca ccggcttggt tgcgccttcg ccgctgccgg cattcagtgc aaataccaat
     2581 ggaaaaagtt ttgaaattac actcaccgtg ccggatgaag aggcgcttcg ttacgatctg
     2641 gtcgtgctga ccgaatccgc taaacccgcc aaacccgcca gccgcaaaga taaaacgcca
     2701 atggatcggg ccgccatcac ggtaaataac gccgccatca cggtaaataa cgccgcggtc
     2761 gagccgcaaa gagaccgcgc cggcaccggc tttgtcatct cctcctttga tctccgcccc
     2821 tatcgcggac agcagattgt catcaagggc ggcggaacaa acaacctgac cctccggcca
     2881 gcggtctggc tggtatgcga ccgtccggtt aaagaacctg cgcccccgga aaaccggatt
     2941 ctgccgatgc aaatatcccg cggatatcgc catcaaagcc agctgctgat tgcaccggaa
     3001 aaataacggt tattccataa aacggctgat ggccgaagcg gtcttcgccg ccattacggc
     3061 gaaggctgat taagtatttc cttttcttcc gctgaatcag taggctcggc ggcctgatga
     3121 aaaataaacc gctgattctc gaagagctga tcatctggct gcgcggacgg ttcacgatga
     3181 ccgacgaaga aaagacctgc atgctgctca tcctcgtcat ctgctgggtc ggcctggtca
     3241 gccgttactt ttacctgaag aaccaggctc cgccggcggc agaaaaactg atagaggatg
     3301 gagacgtttc acagaaggaa acaaagtaaa tgaagcgccg cattcttcgg tgttgtccat
     3361 ccgtcttatt tgacttctac actggttttc ttttttcgtg cctttttgtg ctttttgtgg
     3421 ctgattcttg cgcatgatta aagcggttat ttttgatttc gacggcatca tcgtagactc
     3481 cgaacggctc cactgggcgg ccttcaacaa agtcctcgag cccctcggca gaaccatttc
     3541 atgggccgac tacattaaaa ccttcatcgg cttcgacgac cgcgacacat tccgccacg
//
LOCUS       DAOPZO010000161         3544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_35358_length_3544_cov_7.035827, whole genome shotgun sequence.
ACCESSION   DAOPZO010000161 DAOPZO010000000
VERSION     DAOPZO010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35358_length_3544_cov_7.035827"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1273)
                     /locus_tag="PKI68_08550"
     CDS             complement(<1..>1273)
                     /locus_tag="PKI68_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53779.1"
                     /translation="VEPLSDLLEPLRRAFGGNSAELAKLLSGDLAMWQAAAEGSPRQS
                     FCFYIGITDASQVKELIKSLKLRATETEGICTLASSQGVAWSVQSNALLIASDRACLK
                     ASLPAGAPSLTDTPDFQVFLKKMPGNYAGLKYATYEQGLSVRNAEKLPAPLKPVLELF
                     KGCREMQVSRAENGWLESQSVQHFPAGWPAVRDVLKAAFSAGWTAEKPAAPVPPPLTL
                     SADEVWQKSIDLLSDGRQSDAEKLIDQAVQQYSDEARILFAKAVLERSRWFIDEANRC
                     FAQLVKRFPDTDWAQAARLSLELDRHEAVEADLAALIQLAETSPDNIYLLWLSAIQCR
                     EQNNGILGRRQYEKLLSKFRVGPVLLHQTYANILEENLMDYETALKHRYLAVSMEAKH
                     WSMRGLADTLVKLKRCDEACAVLARTVQLEPS"
     assembly_gap    1274..1373
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1374..2128)
                     /locus_tag="PKI68_08555"
     CDS             complement(<1374..2128)
                     /locus_tag="PKI68_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53780.1"
                     /translation="MKTRLWIGISVAAAGVAAAALFFYFRPAAVPPAETLLPKDTIYY
                     CGTRSPLKVKAAVAALDLQKILQESAGLTEADSRTVSDWISRVQGIHAGFRSFTLVPM
                     VLDAAVILEGRFDRPLTGILSPALQQKIQAEEPYRGVAFRKMLLPIRKNFAMELYITD
                     PVQDRILVAFSRRTLTEMIDRMQDGGPSLAGHPAFREMLARKELRRADLIQYVDTAAF
                     LGLFRDWAKSMPQPQFKTLVDLVWNELRLADYG"
     gene            complement(2142..3371)
                     /locus_tag="PKI68_08560"
     CDS             complement(2142..3371)
                     /locus_tag="PKI68_08560"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX53781.1"
                     /translation="MKQSFSLFLAFKYLKPKRSFLSVVTLLSLLGVTLGVAVLIIVLS
                     VMTGFDETWRERILSFNAHVKVSEYGGVVRQPDKVLSALKKVKGVTGAAPNLEGLVFV
                     QVGGNAQPAQLRGIDPELEKTVSQVPNHMVEGQFSVGRGQIVVGRDLALHCGLRMGDK
                     LLVYSPQNFVSKDEFRLPEELTVSGIFEVGMYEIDSGYVLTSLQTARSIYNVEQGVHS
                     VQVMTADPMQAPVIARDIHKALGPFFDARTWIDLNRQLFTALQTEKNMMFFLLIFITI
                     VAAFGITNTLITLTVQKTHEIGLLKAIGFSNRRIVAIFLWIGMVQGVIGTGMGLGLGL
                     LALKYRNELLNFISSQFRVDLLPKELYQLSQIPSHTTAQDVVTVCATVLVICTLAGLV
                     PAWRAARLEPVEALRNE"
BASE COUNT          783 a         1015 c          948 g          698 t
ORIGIN      
        1 atgagggctc taactgaacc gtccgcgcaa gaacagcaca cgcctcatcg caccttttaa
       61 gctttactaa tgtatcggcg agcccccgca tcgaccagtg cttggcttcc atggaaaccg
      121 ctaaatagcg atgtttcagc gcggtctcat aatccatcag attttcctct aaaatattgg
      181 cataggtctg atgcagcaac accgggccga cacggaattt tgaaagaagt ttttcatact
      241 gtctgcgacc aagaatccca ttattctgtt cgcggcactg gatggcggag agccaaagaa
      301 ggtaaatatt atcgggactg gtttccgcca actgaatcaa ggccgcaaga tcggcctcga
      361 cagcttcgtg ccgatcaagt tccagcgaga gtctggcggc ctgagcccag tcggtgtctg
      421 gaaaacgttt caccagctgc gcaaaacagc ggttggcttc gtcaatgaac caccgtgatc
      481 gctccagtac ggctttggca aataaaatac gggcctcgtc cgaatattgc tgaaccgcct
      541 gatcgatcaa tttttcggcg tcactctgcc gcccgtcact caacagatcg atggacttct
      601 gccacacttc atcggcagat agcgtcagtg gcggcggaac cggtgcagcg ggtttttccg
      661 cagtccagcc ggcagagaag gccgctttaa gaacatcacg aacggcaggc catccggcgg
      721 gaaaatgctg aaccgactgc gactccagcc agccgttttc ggcccggctc acctgcattt
      781 cccggcagcc tttgaataac tccagtaccg gtttcagcgg cgcgggcagc ttttccgcat
      841 tccgcacaga aagtccctgc tcgtaggtcg cgtatttgag tcccgcgtag ttgcccggca
      901 tcttttttag aaagacctga aaatccggcg tgtcggtcag cgacggcgct ccggcgggca
      961 gcgaggcctt caagcaggcc cgatcactgg caatcagcag cgcattggat tgtacgctcc
     1021 atgcgacgcc ttgggagctg gcgagcgtgc agatgccctc cgtttccgtc gcccgcaact
     1081 tcagggattt aatcaactcc ttgacctgcg aggcatcggt aatgccgatg tagaagcaaa
     1141 acgactgtct gggagacccc tcggcagcgg cctgccacat cgccagatcg ccgctcagca
     1201 gttttgccag ctcggcggag ttcccgccaa acgcccggcg cagcggttca agcaggtcgc
     1261 tcaacggctc aacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntacccgt
     1381 aatccgccag ccgcagttca ttccaaacca gatcgaccag cgttttgaat tgcggttgcg
     1441 gcatggattt cgcccagtcg cggaacaggc cgaggaaggc ggcggtgtca acatattgaa
     1501 tcagatcggc gcggcgtagc tccttccggg ccagcatctc ccggaacgcc ggatggccgg
     1561 ccagcgacgg gccgccgtcc tgcatccggt cgatcatctc cgtcagcgtg cgccggctga
     1621 acgccacgag gatgcggtcc tgcaccggat cggttatgta cagttccatg gcaaaattct
     1681 tgcggatcgg cagcagcatt ttgcggaacg ccacgccgcg gtacggctct tccgcctgta
     1741 tcttctgctg cagcgctgga ctcagaatcc cggtcagcgg ccggtcgaag cggccctcca
     1801 aaataacggc ggcgtccagc accatcggaa cgagggtaaa ggagcggaat ccggcatgaa
     1861 tgccctgcac tctgctaatc cagtcgctga cggtccggct gtccgcttcc gtcagcccgg
     1921 ccgactcctg caatattttc tgcagatcca gcgccgcgac agctgctttg actttcagcg
     1981 gcgaacgggt gccgcaataa taaatcgtat ccttgggcag caacgtttcg gcgggcggaa
     2041 ccgctgccgg gcggaaataa aaaaacagcg ccgccgcagc gacccctgcc gccgccacac
     2101 tgattccgat ccaaagtctc gtcttcatct ccactcctcc attattcgtt tcgaagcgct
     2161 tcgaccggtt cgagacgcgc ggcccgccag gccggaacaa ggccggcaag cgtacaaatg
     2221 actaagacag tagcgcaaac agtgacgacg tcctgcgccg tcgtgtgcga cggaatctgg
     2281 ctgagctggt aaagctcttt gggaagcagg tcgacgcgga actggctgga aatgaagttg
     2341 agcagttcgt tgcggtattt cagcgccagc agaccaaggc ccagtcccat tcccgtgccg
     2401 atgacacctt gcaccatgcc gatccagaga aatatggcaa cgatgcggcg gttggagaat
     2461 ccgatggctt tcaagaggcc gatttcatgt gtcttctgca ctgtgagtgt aatcagcgta
     2521 ttggtgattc cgaacgccgc aacgatggtg ataaagatca gcaggaagaa catcatattc
     2581 ttttccgtct gcagcgcggt gaagagctgg cggttgaggt cgatccaggt gcgcgcatca
     2641 aagaacgggc cgagcgcctt gtgtatatca cgggcgataa ccggcgcctg catggggtcg
     2701 gccgtcatca cctgaacact gtggacgccc tgctccacat tataaatcga tcgtgcggtc
     2761 tggagagaag tcagcacgta accggaatca atttcataca tcccgacttc aaaaataccg
     2821 ctgacggtca gctcttccgg cagacggaac tcgtctttcg aaacaaaatt ctgcggtgaa
     2881 tagaccagca gtttgtcgcc catccgcaaa ccgcagtgga gcgcaagatc tcgtccgacg
     2941 acaatctggc cgcggccgac ggaaaactgt ccttcgacca tgtggttcgg tacctgactg
     3001 acggtttttt ccagctcagg atcaatcccg cgcaattgag ccggctgcgc attgccgccg
     3061 acctgaacaa aaaccagtcc ttccaggttg ggtgccgcgc cggtaacgcc tttcactttc
     3121 ttcagagcgc tgagtacctt gtcaggctgc ctcaccacgc cgccgtattc agagactttg
     3181 acgtgcgcgt tgaagctgag gatccgctcg cgccacgttt cgtcgaaacc ggtcatcacc
     3241 gaaagaacga tgatgagcac ggccacgccg agggtgacac cgagaagcga aagcagcgta
     3301 acgaccgaaa gaaacgaccg cttgggcttc agatacttaa acgccagaaa taaggaaaat
     3361 gactgtttca tgaatgagcg gcatcatacg cagacatgcg ctgaaagcaa agtatccagt
     3421 ggcttgaaaa acatgccggt ttcccggcac catatgaaaa agccgcatgt ttccagatga
     3481 ggaagagccg gctcttcctc aaagacgggg gcaagggcga tccctgactg ctacaatagc
     3541 agtt
//
LOCUS       DAOPZO010000162         3462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_36622_length_3462_cov_9.890226, whole genome shotgun sequence.
ACCESSION   DAOPZO010000162 DAOPZO010000000
VERSION     DAOPZO010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36622_length_3462_cov_9.890226"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PKI68_08565"
     CDS             complement(<1..278)
                     /locus_tag="PKI68_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53782.1"
                     /translation="MNTELKKILQKPATGSVLTVAAGISFFFNCMLLPLVGRAGSNAP
                     HADTNRLAFLLVLGITFLLAALATWSKLVRRADDQSPFPYWSLGLCAV"
     gene            376..>1722
                     /locus_tag="PKI68_08570"
     CDS             376..>1722
                     /locus_tag="PKI68_08570"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNX53783.1"
                     /translation="MSEKWITVTGAREHNLKNITVRIPRNKLTVITGLSGSGKSSLAF
                     DTLYAEGQRKYVESLSAYARQFLEQMQKPDVDSIEGLSPAISIEQRTAGSNPRSIVAT
                     TTEIHDYLRLLFSSIGKVHCPSCGKPIQRQSAENITEQLLQIPEKTKVLLLAPLVRGR
                     KGQHEEIFAAIRRQGFVRVRVNGELCELDAVPPLDKNKKHTIEAVIDRLVIDPAARSR
                     INDSVELALATGEGLMIAMVEVGTDRRSVRGAPGGRALPENNWEDRLYSEKNACLDCN
                     ISFETFTPKHFSFNSPYGACQTCHGLGNLLTFDEELVVPDETLPLEEAIHPWRRGGRR
                     AIIYYKKLLAAVADHYGVSLNTPFNELPASFKKTLFYGSGDEPVSYYLRRHKVQKPFE
                     GLIPNLQRSYTESESEFNQERLRAYMGNQRCTACNGARLRPETLACTVGGKNIYEIC"
     assembly_gap    1723..1822
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1823..3265
                     /gene="uvrA"
                     /locus_tag="PKI68_08575"
     CDS             <1823..3265
                     /gene="uvrA"
                     /locus_tag="PKI68_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNX53784.1"
                     /translation="KEIQARLGFMMDVGLDYLTLDRESGTLSGGEAQRIRLATQIGSR
                     LTGVLYVLDEPSIGLHQRDNDRLLATLKELRDLGNTVVVVEHDEQTIREADYVIDLGP
                     GAGVHGGEIVFAGTPAQLLKAPGSPTAQYMRREIQIETPEQRTKASAGYIEIRGAAEN
                     NLKNVNVKIPLGLLTCVTGVSGSGKSTLVDDVLRRALYRRFFGSKERPGKHKELLGVE
                     QIDKVIVIDQSPIGRTPRSNPATYTGAFSIIRDLFTALPASKVRGYGPGRFSFNVKGG
                     RCEHCQGDGLLKIEMHFLPNVYVTCPQCGGKRYNRETLEVHYGGKNIAEVLEMTINEA
                     CEFFAAIPGIERKLRTLRETGLGYLCLGQSATTLSGGEAQRIKLSAELSKKATGKTLY
                     ILDEPTTGLHFADVHKLIEVLETLRDGGNTVLVIEHNLDVIKRADWLIDLGPEGGERG
                     GKIVVAGTPEKVAGTPESYTGKYLAEMLGA"
BASE COUNT          770 a         1104 c          898 g          590 t
ORIGIN      
        1 caaacggcgc aaaggccaag cgaccaataa ggaaacggac tttggtcgtc cgcgcggcgc
       61 accagctttg accacgttgc caaagccgcc agcagaaagg tgattcccag caccagcaga
      121 aacgccagcc ggttcgtatc tgcatgggga gcattggagc ccgcccggcc gaccagcggc
      181 agcagcatgc agttgaaaaa gaaagaaatg ccggccgcca ctgtcagcac actgccggtg
      241 gccggtttct gaagaatctt ttttaactct gtattcatgg ccgttcgtta taacccaaaa
      301 ctggcgccaa acaagatttt tccaaacagc ggaaaggggc tatagtcgct tctgaaatta
      361 cggaaccgtc tgaatatgag cgaaaaatgg atcactgtaa cgggggcacg cgagcataac
      421 ctcaaaaaca tcaccgtgcg catcccccgc aataaactca ccgtcatcac cggcctgagc
      481 ggttcaggca aatcgtcgct cgccttcgac acgctgtatg ccgaaggcca gcgcaaatat
      541 gtcgaaagcc tctccgccta cgcccgccag tttctcgagc agatgcagaa gccggatgtg
      601 gattcgatcg aagggctctc gcccgcgatt tccatcgaac agcgcaccgc cggctccaat
      661 ccgcgctcca tcgtcgccac caccaccgaa attcacgact acctccgcct gctcttttcc
      721 agcatcggca aagttcactg cccgtcctgc ggcaaaccga tccagcggca gagcgccgaa
      781 aacatcaccg aacagctttt gcagattccg gaaaagacca aggtcctgct gctcgcgcca
      841 ctggtgcgcg ggcgcaaggg ccagcacgaa gagatcttcg ccgcgatccg gcggcagggc
      901 ttcgtccgcg tccgcgtcaa cggcgaactc tgcgagctgg atgccgttcc gccgctcgac
      961 aaaaataaaa agcacactat cgaagccgtg atcgaccgct tggtcatcga ccccgccgca
     1021 cgcagccgca tcaatgattc ggttgagctg gcgctcgcca ccggcgaagg gctgatgatc
     1081 gcgatggttg aggtagggac ggatcgccga tccgtccgcg gcgcgcccgg cggtcgcgcc
     1141 ctacccgaga acaattggga agaccgtctt tactccgaaa aaaacgcctg cctcgactgc
     1201 aacatcagct tcgagacctt cacgccgaag cacttttcct tcaacagccc ctacggcgcc
     1261 tgccagacct gccacggcct cggcaacctg ctgacgttcg acgaagagct ggtcgtcccc
     1321 gacgaaacgc tcccgctcga agaagccatc cacccgtggc ggcgcggcgg gcggcgggcc
     1381 atcatctatt acaagaaact gctggccgcc gtcgcggatc attacggtgt ttcgctcaat
     1441 acgccgttca acgaactgcc ggcttccttc aaaaaaactc tcttctacgg ctccggcgac
     1501 gaaccggtca gctactatct gcgccgccac aaggtgcaaa agccgttcga agggctcatc
     1561 ccgaacctcc agcggagcta caccgaatcg gaaagcgaat tcaatcagga gcggctgcgc
     1621 gcctatatgg gcaaccagcg ctgcaccgcc tgtaacggcg cgcgcctgcg gcccgaaacg
     1681 ctggcctgca ccgtcggcgg aaaaaacatt tacgaaatct gcnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnaaggaaat tcaggcgcgc ctcggcttta tgatggacgt
     1861 cggcctcgac tacctcacgc tcgaccgcga aagcggcacg ctctccggcg gcgaggccca
     1921 gcgcatccgg ctcgccacgc agatcggctc gcgcctgacc ggcgtgctct acgttctcga
     1981 cgaaccgagc atcggcctgc accagcgcga taacgaccgg ctgctcgcca cgctcaagga
     2041 actgcgcgac ctcggcaata ccgtcgtcgt cgtcgagcac gacgaacaga cgatccgcga
     2101 agccgactat gtgatcgacc tcggccccgg cgcgggtgtc cacggcgggg aaatcgtctt
     2161 tgccggcacg cccgcccagc tgctcaaagc gccgggatcg cccaccgcgc aatatatgcg
     2221 ccgcgaaatt caaatcgaaa cgccggagca gcgcaccaaa gcctccgccg gctacatcga
     2281 aatccgcggc gccgccgaga acaacctgaa gaacgtgaat gttaaaatcc cgctcggact
     2341 gctcacctgc gtgaccggcg tttccggctc cggcaaaagc acgctggtcg acgacgtctt
     2401 gcgccgcgcc ctctaccgcc gcttcttcgg ctccaaagag cgccccggaa aacacaagga
     2461 actgctcggc gtcgaacaga tcgacaaggt catcgtcatc gaccagtcgc cgatcggccg
     2521 cacgccgcgc agcaatccgg ccacctacac cggagcattt tcgatcatcc gcgacctttt
     2581 cacggcgctg cccgcctcca aggtgcgcgg ctacgggccg gggcgtttca gctttaacgt
     2641 caaaggcgga cggtgcgagc actgccaggg cgacggcctg ctgaaaatcg aaatgcactt
     2701 tctgccgaac gtttacgtca cctgcccgca gtgtggcgga aagcgctata accgcgaaac
     2761 gctcgaagtc cattacggcg gcaagaacat cgccgaagtg ctcgaaatga ccatcaacga
     2821 ggcgtgcgaa tttttcgccg ccatccccgg cattgagcgc aaactgcgca cactccgcga
     2881 aaccggactc ggctacctct gcctcggcca gtccgccacg acactttccg gcggcgaagc
     2941 ccagcgcatc aaactttccg cggagctgag taaaaaagcg accggcaaaa cactctacat
     3001 tctcgacgag ccgacgaccg gcctgcattt tgcagatgtc cacaaactca tcgaagtgct
     3061 ggaaaccctg cgcgacggcg gcaataccgt gctggtgatt gagcataacc tcgacgtgat
     3121 taaacgcgcc gactggctga tcgacctcgg ccccgagggc ggcgagcgcg gcgggaaaat
     3181 cgtcgtcgcc ggaactcccg aaaaagttgc cggaactccc gaatcctata cgggaaaata
     3241 tctggctgag atgctgggag cttgagaagc cgcaaatatt tttacccgtg gagaaaggcg
     3301 ttcggccttt tttgctactt cgctccgtga tatctaaacg ctcgtctaaa attattatct
     3361 tcagcctctg ctattgttga tacaaaacac tcaccgcgat ccagctcaac tatcgtcttg
     3421 tcgtattgct gatcaaatgg cagatgatat attctttctt ta
//
LOCUS       DAOPZO010000163         3443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_36926_length_3443_cov_8.829988, whole genome shotgun sequence.
ACCESSION   DAOPZO010000163 DAOPZO010000000
VERSION     DAOPZO010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36926_length_3443_cov_8.829988"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..1151
                     /locus_tag="PKI68_08580"
     CDS             87..1151
                     /locus_tag="PKI68_08580"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HNX53785.1"
                     /translation="MEQQNANTSPRKGKQLNRDERIQIEVLLKRGSSPKQIASLLGRH
                     VRTIEREVGRGSVEHLHSDLTRSAVYSSDRGEAVHRLNATAKGPPLKLGRHYQTAEFI
                     RFYIVEKRFSPDVVAGLMKQQAMACTLCTKTIYSYIEDGWIAGVTNESLWEKRKRRRK
                     THKRLFRRAKRAASAGRGIADRPSAVSARAEFGHWEIDLIVSGKDTGPGALMTLVERK
                     TRKVIVRKLKDRTQQSVTQALNGIERSMGKEAFRTFFKSISADNGSEFLHAEALETSA
                     LGGHRRTRLYYAHPYASWERGSNENTNRIIRRFLPKGISLSRVTRPALEQIEDWINTC
                     PRRILGFKTAEELFIQEIAA"
     gene            complement(1235..1696)
                     /locus_tag="PKI68_08585"
     CDS             complement(1235..1696)
                     /locus_tag="PKI68_08585"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNX53786.1"
                     /translation="MSLADEIQKLNDLKQAGAISEQEYQQAKDKLLGTSGQPGPRPAL
                     DANSWGLFIHLSQFFAYLLPLAGLIVPLILWQIKKDESALIDRHGKIVMNWLLTEFIL
                     LLIIIPLCFILIGFPLLFILALLGIVFPIIGAIKAGNGEVWPYPCSIRFFK"
     gene            2010..3311
                     /locus_tag="PKI68_08590"
     CDS             2010..3311
                     /locus_tag="PKI68_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNX53787.1"
                     /translation="MKSQNVFAALIEPLQRAVAEEGYTTPTPIQQQAIPHLLEGRDLL
                     GCAQTGTGKTAAFTLPLLQEMTQNKKTAPGGKPRALILTPTRELAAQIGDSIKTYGRH
                     LNVTHTVIFGGVGQNPQVQAMRRGLDVVVATPGRLLDLMAQGHIKLDQIELFILDEAD
                     RMLDMGFIPSVRKVIAKLPAKRHSLFFSATMPPVVAGLAKELLRDPVQITIDPGQPTV
                     ERIGQKLMFVDKENKDELLIDLITDHNMDKVIVFTRTKHGADKVVRQLMSAGISAAAI
                     HGNKSQNARTTALHNFKTGKLRALIATDIAARGLDVDGITHVVNYDLPEEPETYVHRI
                     GRTARAGTDGDAVAFCAARERDFLRDIEKMIRKSIPVDKTHKYHSEDARNATGAAARP
                     EPKGQRGQRRFSGPSRGPSQGHAPRPAHRGGSFSPRRSPAR"
BASE COUNT          792 a          991 c         1003 g          657 t
ORIGIN      
        1 tttaaatgcc gcatccggaa acggagtgtc ccatgccaga atgaactttc cttcattcac
       61 cacaaacgaa agggaagatc accggcatgg aacagcaaaa cgctaacaca tctccgcgca
      121 agggcaagca gttgaaccgc gacgaacgca tccagatcga agtgctttta aagaggggct
      181 cttctcccaa acagatcgcc tctctgctgg gccgccatgt gcgcaccatc gaacgggagg
      241 tcgggcgcgg atcggtggag caccttcata gcgacctgac ccgttcggcc gtctacagca
      301 gcgaccgggg agaggccgtt caccgcctca atgccaccgc caaagggccg ccgctgaagc
      361 tggggaggca ttaccagacg gccgagttca tccggtttta tatcgtggag aagcgctttt
      421 cgcccgacgt ggttgccgga ctgatgaaac agcaggcgat ggcctgtacg ctctgcacca
      481 agacgatcta ttcctatatc gaggacggct ggatcgccgg tgtcaccaat gaatcgcttt
      541 gggagaaacg caaacgccgc cgaaaaacgc ataagcggtt gttccgcaga gcaaaacgag
      601 ccgcgtctgc gggccggggg atcgcggacc ggccgtccgc cgtctccgca cgcgcagagt
      661 tcggccattg ggaaatcgat ctgatcgtca gcgggaaaga tacgggcccg ggggccctga
      721 tgacgctggt ggagcgcaaa acccgcaaag tgatcgtccg caaactcaaa gaccggacgc
      781 agcagtcggt cacgcaggcg ctcaacggaa tcgaacggtc catgggcaag gaagcctttc
      841 gcaccttctt caaatccatc tcggcagaca acggcagcga gttcctccat gccgaggcgc
      901 tggagacctc cgcactcggc ggccaccggc ggacgcggtt gtattatgcg catccctacg
      961 catcctggga gcggggttcg aacgagaata ccaaccggat cattcggcgt tttctcccca
     1021 aagggatctc cctgtcgcgg gtgacccgcc cggcgctcga acagatcgag gactggatca
     1081 acacctgtcc gcgccggatc cttggattta aaacagcaga ggaactgttc atacaggaga
     1141 tagccgcatg aaaagcatcg cgtccgaaaa aatgcggcat ttaaacttac aatctaccgc
     1201 cttaatccgg cctcgccgtc caaaatttag aaagctattt aaaaaaccgt atcgaacacg
     1261 gataaggcca gacttcaccg ttaccggctt tgatggcgcc gatgatcggg aagacgatgc
     1321 cgagcaaggc cagtatgaac agcagcggaa agccgatcag gataaagcag agcggaataa
     1381 tgatcagcag caggatgaac tcggtaagca gccagttcat gacgattttg ccgtgccggt
     1441 cgatgagggc ggattcgtct tttttgatct gccagaggat gagcgggacg atgagtccgg
     1501 ccagcggaag caaataggcg aagaactgcg agaggtggat gaacagtccc caggagtttg
     1561 catccagtgc aggacgcggt ccgggctggc cggaggttcc cagcagttta tctttagcct
     1621 gctgatattc ctgttctgaa atggctccgg cctgtttgag gtcgttcagt ttctgaatct
     1681 cgtcggcaag gctcatgggg tctccttttg agtgggtgca gaacgacgtg tttttaacac
     1741 cccgcagggc gggacgcaac cgttcaaaac ggatgtctca ggtggaacgc gccgtcccgg
     1801 cgcgttttga atttgcacaa cgtgccggga cggcacgttc cacctttttt gaatcttagg
     1861 ttgacacatc ggcggcataa gcctacaacc cacggctcaa ttcggtctgc acatcgcggg
     1921 ccgcacgctg cggattacgc agtatcttct ttccctacac gtccggcagg cagagatttt
     1981 tgaaatattc cggacggaaa agaaggttca tgaaatcaca gaatgtattc gcggctctta
     2041 ttgagccgct gcagcgcgct gttgccgaag aaggctacac gactcctaca ccgattcagc
     2101 agcaggcgat tccgcatctg ctggaaggcc gcgacctgct cggttgcgcg cagaccggta
     2161 ccggcaaaac cgccgcgttc acgctgccgc tgctgcagga aatgacgcag aataaaaaaa
     2221 ccgcgcccgg cggaaaaccg cgtgcgctga ttctcacgcc gacgcgcgaa ctggccgccc
     2281 agatcggcga cagcatcaag acgtatggcc gtcacctgaa cgtcacccac accgtcattt
     2341 tcggcggcgt tggtcagaac ccgcaggttc aggcgatgcg ccgcggtctc gatgtcgtcg
     2401 tcgccacacc gggccgtctg ctggacctga tggcacaggg ccacatcaag ctcgatcaga
     2461 tcgagctgtt cattctcgac gaagccgacc gcatgctcga catgggtttt attccgtcgg
     2521 tccgcaaggt gattgccaag ttgccggcca aacgccactc gctctttttc tcggcgacga
     2581 tgccgccggt ggtcgccggt ctggcgaagg aactgctgcg cgatccggtt cagattacca
     2641 ttgatccggg tcagccgacg gttgagcgca tcgggcagaa gctgatgttt gttgataaag
     2701 aaaacaaaga cgagctgctg attgatctga tcaccgacca caacatggac aaggtgattg
     2761 tcttcacccg caccaagcac ggtgcggata aagtggtccg gcagttgatg tcggccggta
     2821 tcagcgccgc cgcgattcac ggcaataagt cgcagaatgc ccgcacgacg gcgctgcaca
     2881 atttcaagac cggcaaactg cgcgcgctta tcgcgacgga tatcgccgcc cgcggtctcg
     2941 atgtagacgg catcacgcac gtcgtgaatt acgatctgcc ggaagaaccg gaaacctatg
     3001 ttcaccgcat cggccgcacg gcccgcgcgg gaacggacgg cgacgccgtg gcgttctgcg
     3061 ccgcccgcga gcgcgacttt ctgcgtgata ttgaaaaaat gatccgtaaa tccattcccg
     3121 tggataagac gcacaaatat cattccgaag atgcacgcaa cgccaccggc gctgcggcgc
     3181 ggcccgaacc gaaaggtcag cgcggacagc gccgcttttc cggcccgtcc cgcggaccga
     3241 gtcaaggtca tgcgccgcgt ccggcacatc gcggcggatc attttcgccg cgccggtcgc
     3301 cggcccggta agggtgaagg ctttagacct taggatttag ggtttaattt ttcacagcag
     3361 gggtcactga ggtacagagc ttttttgata caatggaaat gatggcaggg cgaagggaga
     3421 cgcaacgtct cagaaatgat ttt
//
LOCUS       DAOPZO010000164         3396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_37649_length_3396_cov_10.695001, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000164 DAOPZO010000000
VERSION     DAOPZO010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37649_length_3396_cov_10.695001"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2076
                     /gene="aspS"
                     /locus_tag="PKI68_08595"
     CDS             <1..2076
                     /gene="aspS"
                     /locus_tag="PKI68_08595"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNX53788.1"
                     /translation="DVGRRVQLCGWVDAFRDHGGLLFIHLRDRSGIMQIVFSPEVAAN
                     DVCRQAAALRSEYCIAVAGEVKKRLAGTENPNIGTGDIELLVTELTVLSEAETLPFSV
                     SEKAMVAGAPSAGSENVAEDLRLQYRYLDIRRPSMRDNLMARHRIFQCVREFLDSKGF
                     LEIETPILTASTPEGARDYLVPSRVHPRNFYALPQSPQLFKQLLMIGGMERYFQLARC
                     FRDEDLRPNRQPEFTQLDIEASFIDEEFLYDLIEELTVKMFAVGGITLSRPFPRMTYA
                     EAMDTTGSDRPDLRFGLRFVDVTDVFAQTSYSIFRQILQRGGCIKGINIKGQSDKLSK
                     NVLQNEYAREIAPSFGAKGMTWMRAEGGKLESNIVQFFSRQELETLQKKFDVVDGDVL
                     IMIADPSYAVVTSALGQLRLHLAKRLGLIPESSFYPLWVTEFPLFEPTDEGGVTSSHH
                     PFTAPDRIDFDPKNIEELLKLRSRAYDLVMNGEELGGGSIRINNRDVQRKIFAALGLT
                     EEETKEKFGFFLRAFDFGAPPHGGLALGMDRTVSMILQTPSIREVIAFPKNRTAACPL
                     TDAPSAVKREQLAELGLLNLGGQDVLPGTAEKENPVDHLSWVSRIGIAAEERPVMEAI
                     LAQAEELAALASQKAGREEPIRTVTPVANRTRPGTQQKRSALAESGQLLKSAPAVKGS
                     YFKVASILE"
     gene            2091..3287
                     /locus_tag="PKI68_08600"
     CDS             2091..3287
                     /locus_tag="PKI68_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNX53789.1"
                     /translation="MNSIFFYRNPETTAPGDGNLQGLKIAIQPNISVAGWPTDAGSNA
                     LFNFTALEDATLVERLHRDGASLCGSLRTSEFGFGLAGNKAGAAVKSQEADAELVMDL
                     MGESRLAAALASACGFKPSYGLVSRYGLVGLIPSMECCGILSGSLKNIREILKSIAGP
                     DERDFSLPEEKAPDFSAQDIDSSKISIGVIAEAQSALDAGKKEQFIAAVDQLKQEGFN
                     VREMSFPDFSLFMLVHKIVGSVEASSCAGRYDSVRYGQRVPGARNWNDMYLSSRGAAF
                     GTLLKSYLFQGAFFQFQRYEAYEDACRIRARLQKEMQQITTQADFLVLPAASGASSGT
                     DVNLENTYAQFASTAFANVTGQPVLYLPPAPRITAGLQIAGPRLSDGRLLALGEHLLN
                     INRGGK"
BASE COUNT          745 a          952 c          949 g          750 t
ORIGIN      
        1 gacgtcggcc gcagggtgca gctttgcggc tgggtggatg cattccggga tcacggcggc
       61 ttactgttta tccatctgcg cgatcgcagc ggtattatgc agattgtctt cagcccggaa
      121 gtcgcggcaa atgatgtctg ccggcaagcg gctgcgctgc gctccgaata ctgcattgcg
      181 gttgccggtg aagttaaaaa gcgtcttgcc ggcacggaaa atcccaacat tggaacggga
      241 gatatagaat tgctggttac cgaattgacg gtgctctccg aggcggagac gctgcctttc
      301 tccgtttccg aaaaggccat ggttgccggc gccccgtctg cagggtccga aaatgtagcc
      361 gaagacttaa ggctccaata cagatatctc gacatccgcc gtccttcaat gcgggacaat
      421 ctgatggcgc gccaccgcat ttttcaatgc gtccgcgaat ttcttgactc caaaggcttt
      481 ctggaaatcg aaacacccat acttacggcc agcacccccg aaggtgcgcg cgattatctg
      541 gtgcccagcc gggtccatcc ccgcaatttt tacgcgctgc cccagtcgcc gcagctcttc
      601 aaacaactgc tcatgatcgg cggcatggaa cgctactttc aattggcgcg ctgtttccgc
      661 gatgaagacc tgcgccccaa ccggcagccg gaattcaccc agctcgacat cgaagcctct
      721 tttattgatg aagaatttct ttacgacctg atcgaagaat tgacggtcaa aatgttcgcc
      781 gtcggcggca tcactctttc ccgccccttt ccccgtatga cctatgcgga ggcgatggac
      841 acgaccggtt ccgaccgtcc cgacctgcgc ttcggtctgc gctttgtcga tgtgacggac
      901 gtgttcgcgc aaaccagtta ttcgatcttc cgccaaattc tgcagcgcgg cggctgcatc
      961 aaaggcatca acattaaggg ccagtccgac aagctcagca aaaatgtcct gcaaaatgaa
     1021 tatgccaggg aaatcgcgcc gtcattcggc gctaaaggca tgacctggat gcgcgccgaa
     1081 ggcggaaaac tggaatccaa tattgtccag tttttcagcc ggcaggaact ggagacatta
     1141 cagaaaaaat ttgacgtggt tgacggggac gtgcttatca tgatcgccga tccatcttat
     1201 gccgttgtca cgtccgcgct gggacagctg cgcctgcacc tggcgaagcg cctaggcctg
     1261 attcccgaaa gcagctttta tccgctgtgg gtgacggagt ttccactgtt tgaaccgacg
     1321 gatgaaggcg gcgtcacgtc cagccaccat ccctttaccg cgccggaccg aatcgatttt
     1381 gatccgaaaa acatcgaaga gctgctgaag ctgcgttcac gggcgtacga cctcgtgatg
     1441 aacggcgagg aactgggcgg cggcagcatc cgcatcaaca accgcgatgt gcagcggaaa
     1501 atattcgctg cgctggggct gacggaagaa gaaacaaagg aaaaattcgg ctttttcctg
     1561 cgggctttcg attttggcgc tcctccgcac ggcggactgg cgctcggtat ggaccgcacc
     1621 gtgtcaatga ttttgcaaac tccatcaatc cgcgaggtga ttgcgtttcc gaaaaaccgc
     1681 accgctgcct gccccctcac cgacgcaccc tccgcagtga agcgggaaca gctggctgaa
     1741 ctggggctgt tgaatctcgg cggtcaggat gttttgcccg gcaccgcaga aaaagaaaat
     1801 ccggtcgatc atctgtcctg ggtttcccgc atcggcattg ccgccgagga gcgccccgtg
     1861 atggaggcta ttcttgccca ggccgaagaa ctggctgctc tcgcgtccca aaaggcagga
     1921 agggaagagc cgatccgcac cgtcacccct gtcgccaacc gcacacggcc gggaactcaa
     1981 cagaagcgat cagcgctggc cgaatccggg cagctgctca aaagcgcgcc cgccgttaag
     2041 ggcagctact ttaaagtcgc cagcattctg gaataaaggg agatatcgtc atgaattcta
     2101 tttttttcta tcgtaacccc gaaaccacgg cacccggcga cggtaattta cagggattga
     2161 aaattgctat tcagccgaac atctccgttg cgggctggcc gacggatgcc gggtcaaacg
     2221 cgctgtttaa ctttaccgcc ctggaagatg caacccttgt agaacgtctt caccgcgacg
     2281 gggcctcttt atgcggttct ctgcgcacga gtgaattcgg cttcggcctc gcgggcaaca
     2341 aagccggcgc ggctgtaaag agccaggaag ccgatgcgga gctggttatg gacttgatgg
     2401 gcgaaagtcg tctggccgca gctctggcgt ctgcctgcgg tttcaaaccc agctatggcc
     2461 tggtgtcgcg ctatggcctt gtcggtctga ttccgtcgat ggaatgctgc gggattcttt
     2521 ccggatcttt gaaaaatatt cgggagattc tgaagagcat cgccggtccg gacgaacggg
     2581 atttttctct gccggaggaa aaagcgccgg atttttccgc gcaggacatc gattcgtcga
     2641 aaatatccat cggcgtgatt gccgaggcgc aaagcgccct ggatgccggg aagaaagagc
     2701 aatttatcgc tgcggtggat cagctgaagc aggagggttt taacgtccgg gaaatgtcat
     2761 ttcccgattt ttcacttttc atgcttgtcc ataaaatcgt cggctctgtg gaggcatcat
     2821 cctgcgccgg gcgttatgat tcggtgcgct atggacagcg tgtgccgggc gccagaaact
     2881 ggaatgatat gtatctttcc tcccgcggtg cagccttcgg tactctgctg aagagctacc
     2941 tgttccaggg cgcttttttt cagtttcagc gctacgaggc ttacgaagac gcctgccgca
     3001 tccgagcgcg tctgcagaaa gaaatgcagc agatcacaac gcaggcggat tttctggttt
     3061 tgccggcagc aagcggagca tcatccggta cggatgtcaa tcttgaaaat acctacgctc
     3121 aatttgcctc cactgctttt gccaatgtca ccggccagcc cgttttgtat ctgccgccgg
     3181 cgccgcggat aacagcagga ttgcaaattg cggggccgag gctcagcgac ggcaggctgc
     3241 tcgcactggg agagcatctt ctcaatatca accggggagg taaataggaa tggaatttga
     3301 agcggtcatc ggattagaaa tccatgttga actcaattgt gcaaccaagc tgttttgtga
     3361 ttgccccaac aatcccggcg acgaccccaa cgtcaa
//
LOCUS       DAOPZO010000165         3386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_37816_length_3386_cov_10.557190, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000165 DAOPZO010000000
VERSION     DAOPZO010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37816_length_3386_cov_10.557190"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="PKI68_08605"
     CDS             <1..1377
                     /locus_tag="PKI68_08605"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNX53790.1"
                     /translation="LGAGDSRNYALELMKQLEAHKRIDGLSNPNHQSKIIHHKFSTDY
                     LWGEARGQMFGVLECTDAAGNTVVLKAFSAQYNGEWLADGWVPPVLDVNEFNRLVGPV
                     DREIKSLDRQIAETKRPELIQRRKALSQELMKHIHALYLLTNFRGETKPMTEVFRGGI
                     PTGAGDCCAPKLLNYAARNGLKPRGLSEFYWGRENRSGTRKHGEFYPACAEKCQPILG
                     FLLCGFSTEGNKGNQGNSPSFSSFPSVKISTEVEVVYCESEFVVVNKPSGLLAVPGKG
                     KENQDCVVSRIKALYPDCIEQPAVHRLDMDTSGLMVLARTADAHRELSRQFEHRETEK
                     RYIALLDGELSEEAGTLELPFRLDVDHRPLQIYDPVHGKTGITHWKVLSTRNKKTRVE
                     FVPVTGRTHQLRVHAASEHGLGLPIVGDRLYGSGTAPGQLKLHAAFLSFTHPRTGERL
                     EFRSAPPF"
     gene            1542..2762
                     /locus_tag="PKI68_08610"
     CDS             1542..2762
                     /locus_tag="PKI68_08610"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNX53791.1"
                     /translation="MKIKSFQAWRPAEGLADKIATLPYDVMDRKQAAEMAAGNPLSFL
                     RVTRSEIDLPESTDPYSDAVYAKAVENFEKLKTGGSLVREAKPTLYLYSQEMDGRTQY
                     GVVALCNVADYESGKIKIHEKTRRAKEDDRTKHVDRTNSQTGPIFLTYRDQSAITALM
                     QKTAQEAPLYRVTAAGGIIHTVWKIADSAAVQSAFENVPAFYVADGHHRAASAVRVAQ
                     MRRKANPHHTGDESYNWFLTVLFPAGQLKILPYNRVVFDLNGQSQEEFFLGLEKAFTV
                     TATDKKMPDAPGSVCMYIDRKWFLLTPKAALSKDPVASLDVSVLQDRVLAPLLGIDDP
                     RESNRIDFIGGIHGTPELERRVNAGEAAVAFSLYPTTLDQLMAVADANLLMPPKSTWF
                     EPKLRDGLLVNTID"
     gene            2765..>3386
                     /locus_tag="PKI68_08615"
     CDS             2765..>3386
                     /locus_tag="PKI68_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53792.1"
                     /translation="MKGLREIPPEFVTEAKEPSPWDFGNEVLYSLCLNEPSHQKEGAV
                     IAKVWLIGRAYAAAIERRRNKNGKNDAFYVNEVAPAIIRSKIDAWINEATLHKEICDQ
                     SLPVILKAHYKVTQLFCDISGLEKRSLASKYLHFHQPQLFYIYDTRAVTAMRKLSPIV
                     GRDNTCNSSDKYCDIEYRKFSEKCLHLHRHIKEKFQTSLNPRQIDNL"
BASE COUNT          809 a         1005 c          882 g          690 t
ORIGIN      
        1 ctcggtgcag gcgacagccg caactatgcg ctggagctga tgaaacaact tgaagcacat
       61 aaacggattg atggtctttc gaatcccaat catcaatcga aaatcatcca tcataaattc
      121 tccaccgact acctgtgggg cgaagcgcgc ggacagatgt tcggggttct cgaatgcacg
      181 gacgccgccg gaaataccgt cgtgctgaaa gcgttctccg cgcaatacaa cggcgagtgg
      241 ctggccgacg gctgggtgcc gccggtcctc gacgtgaatg aattcaaccg ccttgtcggt
      301 ccggtggatc gcgaaatcaa atcgctcgac cggcagattg cagagactaa acgtccggag
      361 ctgatccagc ggcgcaaagc gctttcgcag gagctgatga agcatatcca tgcgctttat
      421 ctcctgacca acttccgcgg cgaaacgaag ccgatgaccg aagtattccg cggcggcatt
      481 ccgaccggcg cgggcgactg ctgcgcgccg aagctgctga actatgcggc gaggaacgga
      541 ctgaagccgc gcggtctcag cgagttttac tggggcagag aaaaccgctc cggcacccgc
      601 aagcacggcg agttctatcc ggcctgcgcc gaaaaatgtc agccgatcct cggctttctg
      661 ttgtgtggat tttctacaga aggtaacaaa ggaaaccaag gtaacagccc ttcgttctct
      721 tcgtttcctt ctgtaaaaat ttctacggaa gttgaagtgg tctactgtga atctgagttc
      781 gttgttgtga acaaaccgtc cggactgctg gcggtgccgg gtaaaggcaa agagaatcag
      841 gactgcgtgg tcagccggat caaagcgctt tatccggact gcatcgagca gcccgccgtc
      901 caccggctcg acatggatac gtccggactg atggtgctgg cgcgcaccgc cgacgcgcac
      961 cgcgagctgt cgcggcagtt tgagcatcgc gaaacggaaa agcgctacat cgcgctgctc
     1021 gacggcgaat tgtcagaaga ggcaggaacc cttgagctgc ccttccggct cgatgtcgac
     1081 catcgtccgc tccagatcta cgaccccgtt cacggcaaaa ccggcatcac gcactggaag
     1141 gtgctgagca cccgcaataa aaaaaccaga gtggagtttg ttcctgttac cggacgcact
     1201 caccagctgc gtgttcacgc ggcgtcggaa cacggcctgg gacttccaat cgttggggat
     1261 cgcctttacg gttccggcac cgctccggga cagctcaagc ttcacgcggc gttcctgagc
     1321 tttacccatc cgcgtaccgg cgaacggctg gagttccgtt ccgccccgcc gttttaaaag
     1381 aaccacccac gaattacacg aatctcgata gacgaatttt gctttcagcg attccgtata
     1441 tttccaaacg ctcctctcct tctcattcgt gttgattcgt gggcaaattt tccgtcttcg
     1501 cgatttcatt ttcgggcggg ttcggtagac tgcgccgcat tatgaaaatc aaatcgttcc
     1561 aagcatggcg gcccgccgaa ggtctggcgg ataaaattgc gactcttccc tacgatgtga
     1621 tggaccggaa acaggcggcg gaaatggccg ccggcaatcc gctttcgttc ctgcgcgtaa
     1681 cccgttcgga aattgatctg ccggaaagca ccgatccgta ttccgacgcg gtgtatgcca
     1741 aggcagttga aaattttgaa aaactcaaaa ccggcggctc gctggtacgc gaagccaagc
     1801 cgacactcta tctttacagc caggaaatgg acggtcgcac tcagtacggc gtcgtcgcgc
     1861 tctgcaacgt ggccgactac gaatccggca aaatcaaaat tcacgaaaag acccgccgcg
     1921 ccaaagagga cgaccggacg aaacacgtcg accggaccaa cagccagacc ggcccgattt
     1981 tcctgaccta ccgcgaccag tccgccatca cagcgctgat gcagaagacc gcgcaggaag
     2041 cgccgcttta tcgcgtgacc gccgccggcg gaatcatcca caccgtctgg aagattgccg
     2101 attccgccgc tgtgcagtcc gccttcgaaa acgtacccgc tttttatgtc gctgacggtc
     2161 accaccgcgc agccagcgcc gtgcgcgtcg cgcagatgcg ccgcaaagcc aacccgcacc
     2221 acaccggcga tgagtcctac aactggttcc tgaccgtgct cttcccggcc ggacagctca
     2281 aaattctgcc ttacaaccgc gtcgtcttcg acctcaacgg ccagtcgcag gaagaatttt
     2341 tcctgggcct ggaaaaagcc ttcaccgtca cagcgaccga caagaaaatg cccgacgcgc
     2401 ccggctctgt ctgcatgtac atcgaccgta aatggtttct gctgacgccg aaagcggcgc
     2461 tgtcgaaaga tccggtcgct tcgctcgacg tgagcgtatt gcaggatcgc gtactggctc
     2521 cgctgctcgg cattgacgat ccgcgcgaaa gcaaccgcat cgacttcatc ggcggcatcc
     2581 acggaacgcc ggaactcgaa cgccgcgtca acgcgggcga agcggcggtc gccttctcgc
     2641 tctatccgac cacgctcgac cagctcatgg cggttgcgga tgccaacctg ctgatgccgc
     2701 cgaagagcac ctggttcgaa cccaagctcc gcgacggcct gctggtcaac acgatcgatt
     2761 aagaatgaag ggtttaagag aaattccacc agaattcgtt accgaagcca aagaaccatc
     2821 accatgggac ttcggaaacg aagttttgta cagcttgtgc ctcaacgaac catcacatca
     2881 gaaagaaggt gcggttatag ccaaagtctg gttgataggg agagcctatg cagcagccat
     2941 agaacgtcgc cgcaacaaga acggcaagaa tgatgctttc tatgtcaacg aagttgctcc
     3001 agctatcata cggtcaaaaa tcgatgcgtg gatcaatgaa gctactttgc acaaagaaat
     3061 ttgcgaccaa tccctaccgg tcattcttaa agctcactat aaggttacgc aacttttctg
     3121 cgacatcagt gggttagaaa agcgttcgct ggcttcaaaa tatcttcact ttcaccagcc
     3181 tcagcttttc tacatctatg acacgcgcgc cgtcaccgcg atgcgcaaac tatctccgat
     3241 tgtaggccga gacaatacat gtaattcctc tgataaatac tgcgacattg agtaccgcaa
     3301 gttttctgaa aaatgcctgc atctgcaccg tcacattaaa gagaaattcc agacatctct
     3361 taatcctcgt caaatcgaca acctgt
//
LOCUS       DAOPZO010000166         3354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_38336_length_3354_cov_4.419824, whole genome shotgun sequence.
ACCESSION   DAOPZO010000166 DAOPZO010000000
VERSION     DAOPZO010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38336_length_3354_cov_4.419824"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1064)
                     /locus_tag="PKI68_08620"
     CDS             complement(153..1064)
                     /locus_tag="PKI68_08620"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNX53793.1"
                     /translation="MRVLVTGGAGFIGSHVVEHFQKKAEVRVLDNLRSGYLKNLQGLH
                     YEFMEGSVTDPAAVRAAMKGVDYVFHMAAMISVPESMSKPVECVEINTIGTLSVLEEA
                     AKAGVKKLCFSSSAANYGDNPAVPKLETMLPEPKSPYAVTKLDGEYYCEMFTRERKLA
                     TAVMRYFNVFGPRQNPGSAYAAAVPIFISKAVKNETITIFGDGEQTRDFIYVKDIVAA
                     NVFLAMNDTPPGVFNVAYGGKLTINELAKKIIGLTGSSSKIEYAAERSGDVKHSMASV
                     DKIKAAGFKPTSNCDDGLKETIKYFTT"
     gene            complement(1172..2266)
                     /locus_tag="PKI68_08625"
     CDS             complement(1172..2266)
                     /locus_tag="PKI68_08625"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNX53794.1"
                     /translation="MDQLKEVIEAFQCSGKYLRGERYGSGHINDTYKVTCKENSREVH
                     YILQRVNHTVFKDVPELMKNIGQVTRHQRKKFEEAGTQELARRVLTLIPAVDGKDYCR
                     DSKGNFWRTYVFIEGAVGIDVVENTKQAFEAAKAFGAFQGQLADLPGRLTETIPEFHH
                     TRSRFNVLKAAISADVCGRAANIKKEIEFAVSREALVDVIIDRMASGEIPERVTHNDT
                     KLNNVLIDTVTGKGMCVIDLDTVMPGSILYDFGDMVRTTTTTAAEDERDLSRVELDLD
                     YFEALVKGYLETASGFLVPKERELLPFSGKLITFEIGLRFLTDYLQGDVYFKTHREGQ
                     NLDRCRKQFKMVESMERQMEAMRRVADQCK"
     gene            2517..>3354
                     /locus_tag="PKI68_08630"
     CDS             2517..>3354
                     /locus_tag="PKI68_08630"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX53795.1"
                     /translation="MKELRQALLNLKEPEDYFQGIGNEKLPTPMNILLFFRPSREKLQ
                     QEAPQNRSHHRFVLIFNLKTPGCVHVNHLSLRLHPRQALLLHPYQFHHFSHLASNQLM
                     WLICTFELPNDSLLEPLRNQVVDVSRRSEEARNSLLHEWIRCSEPERPTDLQAAQLQA
                     VLTRLLLCLRQDHQAAAGIPPSEEPVGSLIQRVNRQLLEWHEGSMTVGDLAVATGQSE
                     SRLRILFKETAGIPLGGYIQNYRINRAMELLKTSRISIADVAEAAGFGSPQAFCRTFK
                     KQT"
BASE COUNT          725 a          995 c          844 g          790 t
ORIGIN      
        1 aagcggtgta tgtcggcacc aaaacaaaac cgtttctgta cgagccgccg cgccgccggt
       61 ttggccgcgg ctagccagac tcagccgtat tgcatacacc ggctccaacc agaacccgga
      121 ggccagccat attcgttcgt cagacggatc ctttacgttg tgaaatattt gatggtttct
      181 ttgaggccgt catcacaatt ggaggtcggc ttgaagccgg cggctttaat tttgtcgacc
      241 gatgccatgg agtgtttgac atcgccggag cgttcggccg cgtattcgat tttcgacgac
      301 gagccggtca gtccgatgat ttttttcgcc agctcgttga tggtcaactt cccgccgtag
      361 gcgacattga agactccggg gggcgtatca ttcatggcga gaaaaacatt ggcggcgacg
      421 atgtctttca catagataaa atcgcgggtt tgttcgccgt ctccgaagat ggtgatggtt
      481 tcgtttttga ccgctttgct gatgaagatc ggtacggcgg cggcataagc gctgccggga
      541 ttctggcggg ggccgaagac attgaagtag cgcatgacgg ccgtagcgag cttccgttcg
      601 cgggtgaaca tctcgcagta gtactccccg tcaagcttgg tgacggcata cggacttttc
      661 ggctcgggca gcatggtttc gagcttcggc acagccggat tgtcgccgta gtttgcagcc
      721 gagctggaga agcagagttt tttcacgcct gctttcgcgg cctcttcgag cacactcagc
      781 gttccgatgg tgttgatctc cacacattcg accggcttgc tcatggattc agggacgctg
      841 atcatcgcgg ccatatggaa aacatagtca acgcctttca tcgcggcgcg aaccgccgcg
      901 ggatcggtaa ctgagccttc cataaattca tagtgaagcc cctggagatt tttcagatac
      961 ccggaccgca ggttgtcgag cacccgcacc tccgcttttt tctgaaaatg ctccacaacg
     1021 tgactgccga tgaatccggc gccgccggtg actaaaactc tcattgctct ttccccttat
     1081 acctgccgca tcgcatctgt ctgatgctcc gcagatacgc ctgtttggag gagacggctc
     1141 cgctccccgt tgtcaaaccg gcgccccggt ttcatttgca ttgatccgca acacgccgca
     1201 tggcctccat ttgacgctcc atcgattcga ccattttaaa ctgtttgcgg caacgatcca
     1261 ggttctgccc ctcgcggtgt gttttaaaat aaacatcgcc ttgcaggtaa tcggtcagaa
     1321 agcgcagtcc gatctcaaag gtaatcaact tgcccgaaaa gggcagcagc tcgcgctctt
     1381 tcggaacgag aaatccggac gcggtttcca gatacccttt gaccaaagcc tcaaaataat
     1441 ccagatccag ctcgacccgg gaaagatccc gctcgtcttc cgctgcggtg gtcgtggtcg
     1501 tgcgaaccat gtcgccgaag tcgtacagga tcgagccggg catcaccgtg tccagatcaa
     1561 tcacgcacat ccctttcccg gtgacggtgt caatcagcac attgttaagc ttggtatcgt
     1621 tgtgcgttac gcgctccgga atttccccgg aggccatccg atcaatgatc acatcgacca
     1681 gcgcttcgcg actcacggcg aattcgatct cctttttgat attggccgcg cggccgcaga
     1741 catcggcaga gatcgccgcc ttgagaacat taaagcgcga acgggtatga tggaattcag
     1801 gaatcgtctc cgtcaaccgc ccgggcagat ccgccagctg gccttgaaag gctccgaagg
     1861 ctttggcggc ctcgaacgcc tgcttcgtgt tttcaaccac gtcgatgcca acggcacctt
     1921 cgataaaaac ataggtgcgc cagaaattcc cttttgaatc ccggcagtag tctttcccgt
     1981 ccacggccgg aataagcgtc agaacccgtc gggccaactc ttgcgtcccg gcctcctcga
     2041 attttttgcg ctggtgtcgc gtaacctgtc cgatattttt catcagctcg ggcacatctt
     2101 tgaaaacagt atggttcacc cgctggagaa tatagtgcac ctcgcggctg ttttctttgc
     2161 aggtcacctt gtaggtgtca ttgatatgtc ccgagccata ccgctctccg cgcaaatatt
     2221 ttccggaaca ttggaaggct tcaattacct ctttcaactg atccatgttt tttactccgt
     2281 ctttcgtacc ttacgtgcag aaccttcacc gtacgcttcg cggccgcaga accgccggga
     2341 tccattcgac atccccaagc ggcgcaacca cggcttctgc tccaatctaa gaaaatatca
     2401 caatctggag cgaagaatgg cacccccgtt ttccgccgga aaggccaatt aattaccaca
     2461 tttaactgct ttttaacttt attgacttcg ggccgttctg cgacattatg ctccggatga
     2521 aagaactccg tcaggcgctt ctaaacctca aagagccgga agattatttt caagggatcg
     2581 gcaacgaaaa gctgccgact cccatgaata ttctgctgtt tttccggccc agccgcgaaa
     2641 aactccagca ggaagcgcct caaaaccgct cacatcaccg atttgtgctg attttcaacc
     2701 tcaaaacccc gggatgcgtc cacgtcaatc atctctccct gcggctgcat ccccggcagg
     2761 ctctgctgct tcacccctat cagtttcatc acttcagcca cttggcatca aatcagttaa
     2821 tgtggctgat ctgcaccttc gaactgccga acgactcact gctcgaaccg cttcgcaacc
     2881 aggtcgttga cgtaagccga cgttcagaag aagcgcgcaa cagtcttttg cacgaatgga
     2941 tccgctgttc cgaaccggag cggccgacgg atcttcaggc cgcacaattg caggccgtat
     3001 taacccggct gctgctatgc ctccgtcagg atcatcaggc ggccgcaggc atcccgccgt
     3061 ccgaagaacc cgtcggcagc ctgatccaaa gagtcaaccg ccagctgctc gaatggcacg
     3121 aaggaagcat gaccgtcggc gatctagccg tcgccacagg acagtctgaa tcccgtctgc
     3181 ggatcctgtt taaggaaacg gcgggaattc cgctcggagg ttacattcaa aactaccgga
     3241 tcaaccgtgc catggagctg ctcaaaacct cccggatctc gattgccgac gtggccgagg
     3301 ccgccggctt tggatcgccc caggcttttt gccgaacctt taaaaaacaa accg
//
LOCUS       DAOPZO010000167         3325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_38821_length_3325_cov_13.628440, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000167 DAOPZO010000000
VERSION     DAOPZO010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38821_length_3325_cov_13.628440"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1249
                     /gene="ychF"
                     /locus_tag="PKI68_08635"
     CDS             149..1249
                     /gene="ychF"
                     /locus_tag="PKI68_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNX53796.1"
                     /translation="MSLSIGILGLPNVGKSTIFNALTRAQNAEAANYPFCTIEPNKAV
                     VPVPDERVDKLATMANPKKVVYATVDFVDIAGLVKGASRGEGLGNKFLANIREADALL
                     HVVRCFADENVVHVDGSVDPMRDIDVIETELVLADLQTLETRAQRMEKLARNDKKVAE
                     QLAAVKRLIDHLGEGKPAVSFPEHDTDDMKALLKESPLLTDKKIIYCANVDESGMAED
                     SAYVKAVRAYADSKGADTVKICAKMEEDLIGMDDAEAAEFLKEYNVTESGLDQIARTG
                     YHTLGLISYLTAGPKEVHAWTIHRGWKAPAAAGVIHTDFERGFIRAQVISYNDYITYG
                     GEAGCREKGLMRTEGKDYVVQDGDVIEFLFNV"
     gene            1414..1731
                     /gene="trxA"
                     /locus_tag="PKI68_08640"
     CDS             1414..1731
                     /gene="trxA"
                     /locus_tag="PKI68_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNX53797.1"
                     /translation="MSDKVKDLTAANFTNTVASGTVLVDFWAPWCGPCKMQTPILEKV
                     ADKIGDKAVIAKVNVDESPALAAQFGVRSIPTLILFKNGQKVRDFIGVQQEAALVEVL
                     TAG"
     gene            complement(1832..2608)
                     /locus_tag="PKI68_08645"
     CDS             complement(1832..2608)
                     /locus_tag="PKI68_08645"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNX53798.1"
                     /translation="MTPEKLNRLTERFCTYTDRYRINGQLHPMHQLKLEHTLRVAADA
                     RSIAAGMNWPEEEICLAEAIGLFHDVARFPQFQQYRSFDDHKTVDHGDLGVQTLESEN
                     LLAGVTEESRALILHAVQHHNKKELPPGLTVHEERHLRLIRDADRLDIFFICWDSIRT
                     GHIHDYPEIIINMDFNGPPTPAVLEQFERGEKIDYRGMKSMADRFVMQLSWMHDMSYA
                     STGRLVRERGILDKFISVLPVKTDQLMRCFERTEAFLASA"
     gene            complement(2614..3159)
                     /locus_tag="PKI68_08650"
     CDS             complement(2614..3159)
                     /locus_tag="PKI68_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53799.1"
                     /translation="MLELSTVAMIIGLLALTGGTVSVLAPAKIRAGVAAFPRSIWPGR
                     ILAAIDLVWAAYELAQMHLGMFDAWKVHLYWLTPAAIVLCVVYLDELLSPRALGGLLL
                     LAAGPVLDIARWHSSNWRLVITVLAYLWILLGLVFLLSPWWFRRIALWVTGRGDTAVR
                     VTGAIKAAAGLGLIALALLVY"
BASE COUNT          793 a          928 c          933 g          671 t
ORIGIN      
        1 cggttttgcg gctaacttcc ccaactttcg cccgctattg cgggaagcac taaactcgaa
       61 tttcgaagca cgaatgcaga gttccaagca ttggtatttc tgatttgttt agggtttagg
      121 gtttcggatt tcgaatttgg agaacgctat gagtctgagc attggtattc tgggtcttcc
      181 gaacgttgga aaatcgacga ttttcaacgc gctgacacgc gcacaaaacg cggaggccgc
      241 caactatcct ttctgtacca tcgagccgaa caaggcggtc gtgccggttc cggacgaacg
      301 ggtggataaa ctggcgacga tggccaaccc gaaaaaggtg gtttatgcca ccgtggattt
      361 tgtcgatatc gccggtctgg taaaaggcgc gagccgcggc gaaggcctcg gcaataaatt
      421 cctcgctaac atccgcgaag cggatgccct gctccacgtt gtccgctgtt ttgcggatga
      481 aaacgtagtt catgtggacg gctccgtcga tccgatgcgc gatattgatg tgattgaaac
      541 cgagctggtg ctggccgatc tccagacgct ggaaacgcgc gctcaacgga tggaaaaact
      601 ggcgcgcaac gacaagaagg ttgccgaaca gctggccgcg gtaaagcggc tgatcgacca
      661 cctcggcgaa ggcaaaccgg cggtcagctt tccggagcac gataccgacg acatgaaagc
      721 tctcctgaaa gagtcgccgc tgctgacgga taaaaaaatc atctactgcg ccaacgtgga
      781 cgaatccggc atggcggaag acagcgccta tgtgaaggcc gtgcgcgctt atgccgattc
      841 caaaggcgcg gacaccgtga aaatctgcgc caagatggaa gaggatctca tcggcatgga
      901 cgacgctgaa gcggcggaat ttctcaagga gtataacgta accgaaagcg gcctcgacca
      961 gatcgcccgc accggctacc acacgctcgg tctcatcagc tatctcaccg ccggaccgaa
     1021 agaggttcat gcgtggacga ttcaccgcgg ctggaaggcg ccggcggcgg cgggcgtgat
     1081 tcacaccgac ttcgaacgcg gcttcatccg cgcccaggtg atttcctata acgactacat
     1141 cacctacggc ggcgaagccg gctgccgcga aaaagggctg atgcgcaccg aaggcaaaga
     1201 ctacgtcgtg caggacggcg acgtgatcga gttcctgttt aatgtataag gcctgcggcc
     1261 gttattagat attcgttatc cgttcttaga cgaagcatct ttttcgcccg atatctaata
     1321 acaaataacc atgactgctc cgcagtctta tgcttgttcc tgcaatgcct cccccgtata
     1381 atcagccgca tctaaacgaa gcaggagaac gacatgtctg ataaagtaaa agatctcacc
     1441 gctgccaatt tcacaaacac agttgcctcc ggaaccgtac tggtcgattt ctgggcgccg
     1501 tggtgcggcc cgtgcaaaat gcagaccccg atcctcgaaa aagtcgctga caaaatcggc
     1561 gacaaggccg tgattgctaa agtaaatgtg gatgaaagcc cggcgctggc tgcacagttc
     1621 ggcgtacgct cgattccgac attgatcctc ttcaaaaacg gacagaaagt ccgcgacttc
     1681 atcggcgttc agcaggaagc cgcgctggtc gaagttctga ccgccggata acgcaacaag
     1741 gccggctgga agccggcgat acaaattttg taccgcaggc atcctgcctg ccttgattaa
     1801 atccttacta tcatccggac ccgggggcgt ttcaggcaga tgccagaaac gcctccgttc
     1861 tttcaaaaca gcgcatcagc tggtcggttt tgacaggcag cacgctgatg aatttatcaa
     1921 gaataccgcg ctcgcgaacc agccgcccgg ttgaagcata actcatgtcg tgcatccagc
     1981 tgagctgcat aacaaaacgg tccgccatgc ttttcatgcc ccggtaatca atcttttcgc
     2041 cgcgctcgaa ctgctccagc accgccgggg tcggcggccc gttgaaatcc atattgatga
     2101 tgatttcggg gtagtcgtga atatgcccgg tccggatgga atcccagcag atgaaaaaaa
     2161 tatcgagccg gtcggcatcg cggatcagcc gcaaatgcct ttcttcgtgt acggtaagtc
     2221 cgggcggcag ctcttttttg ttgtggtgct gaaccgcatg gagaatcagc gcgcgggatt
     2281 cctcggttac acctgccagc aggttttcgg attctaacgt ctgaaccccg aggtcgccgt
     2341 gatcgaccgt tttgtgatcg tcaaaactgc ggtactgctg aaattgagga aagcgggcca
     2401 cgtcatgaaa aaggccgatt gcttccgcca gacagatttc ctcttccggc cagttcattc
     2461 cggcggcgat ggatcgggcg tcggcggcaa cacgcagggt gtgttccagc ttcagctgat
     2521 gcatcggatg aagctgtccg ttgattcggt aacgatccgt ataggtgcag aagcgttctg
     2581 tcagccggtt gagcttttcc ggagtcatga cgttcagtaa accagcagag ccagcgcgat
     2641 cagcccgagc ccggccgccg ctttaattgc gccggtgact cggacggcag tatcgccgcg
     2701 accggtcacc caaagtgcaa tgcggcggaa ccaccacggc gagagaagga aaaccagtcc
     2761 gagcagaatc cagagataag cgaggacggt gataacaagc cgccagtttg acgaatgcca
     2821 gcgcgcaata tccagtaccg gcccggccgc cagaagcagc agcccgccga gggcgcgcgg
     2881 ggaaagcagt tcgtcgagat aaacgacgca gagaacaatg gcggccggcg tcagccagta
     2941 gagatgaact ttccaggcgt cgaacatgcc gaggtgcatc tgcgcgagtt cataagcggc
     3001 ccagaccaga tcaatcgcgg ccagaatacg gcccggccag atactgcgcg gaaacgccgc
     3061 cacgccggcg cggatttttg cgggcgccag caccgaaacg gttccgccgg tcagcgccag
     3121 taatccgatg atcatcgcaa cagtagaaag ttccaacatc tcgcttctcc ctgattaaaa
     3181 cgggatccac tctgcccgat cccattcatt cctgaaaaca aaaaacgccc cggagttccg
     3241 gggcgttttc gaaagcagaa ttcaaattct gcttactttt tgaggctggc gcggatgctg
     3301 tcgagctgtc cggcgctgcc gagaa
//
LOCUS       DAOPZO010000168         3257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_40030_length_3257_cov_10.322299, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000168 DAOPZO010000000
VERSION     DAOPZO010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40030_length_3257_cov_10.322299"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /gene="rsfS"
                     /locus_tag="PKI68_08655"
     CDS             <1..539
                     /gene="rsfS"
                     /locus_tag="PKI68_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNX53800.1"
                     /translation="AKKTTAKKTAKKTAKAAPKAKVAKAVSKAKTAKAAPKAKVSKAA
                     PKAAARTQPKAPKLSGLASLPDVVQKAVKFLDDRKAENIAVLDLRDLANISEFFVIVT
                     AANAPHLKALGDGLQRLFKNEQYEGYRFAGTGDSGWAIIDYDGVMVHVFSAEMRSLYD
                     LEKLWKDAKRVAIDLKQL"
     gene            601..1017
                     /locus_tag="PKI68_08660"
     CDS             601..1017
                     /locus_tag="PKI68_08660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNX53801.1"
                     /translation="MKSYRKELWFKTPVRRAFINITSEVADALRESGIQEGLCLVNAM
                     HITASVFINDDEGGLHQDFETWLETLAPEKPHSQYAHNGFEDNADAHLKRQIMGREVV
                     CAVTGGKLDFGPWEQIFYGEFDGLRDKRVLVKIIGE"
     gene            1105..>3257
                     /gene="priA"
                     /locus_tag="PKI68_08665"
     CDS             1105..>3257
                     /gene="priA"
                     /locus_tag="PKI68_08665"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNX53802.1"
                     /translation="MNRIAKVAVDLSLDREFDYLIPEPLRSLVAIGSRVEVPFRSRTV
                     TGFVVGFADQSAFDDLKPIGKVLGETSLVTETVMELARWMSAYYLAPFEVCVRAVLPA
                     VVRNKESGGKKQLTVSLVNVADSSSLPPKQQALIECLRENGPMLLSELTEAAGVTAAP
                     VKALEKKGLVTVSNESVYRDPHEGIELLKTAPLALMPQQADALKQIVTAMAEPEPPVL
                     LLHGVTGSGKTEVYLQAIAKALEQEHGAIVLVPEIALTPQTVDRFRARFADHPKQVAV
                     LHSSLSDGERYDEWHRIRSGEAKIVVGARSALFAPVHNLGLIVVDEEHEPTYKQDEAP
                     RYSARDMAVLRGRLEKCAVVLGSATPALESYANAKGGKYRYVDMPIRVDDRRMPPIRI
                     VDMRIAAQQEGKPVLFSRELVEAIRTRIDRAEQTMIFLNRRGFSSSLICPDCGYVAEC
                     EHCSVGMTYHKARARLVCHICGEEKAVPVRCPECKSDQFKYAGAGTEKIEEVMTKLFP
                     KARIERMDSDTMRRKNAHRDILSRFRTGRIDILIGTQMIAKGLDFPNVTLVGVVNPDH
                     ALHMADFRAGERVFQLLTQVAGRAGRGEAAGEVIVQTYTPHHPAIQAARRMDFDGFCE
                     QELAFRKQLDYPPYTHLTCITLRGKTEAEVEAAAEKLFQVLEKCTAGKAVLSPAIPAP
                     IARMRGEYRWQILLRAARTKTMNDSIRAAFGLMKLP"
BASE COUNT          668 a          930 c         1058 g          601 t
ORIGIN      
        1 ccgcgaagaa aacaaccgca aaaaagacgg cgaaaaagac cgccaaggcg gctccgaaag
       61 cgaaagtcgc caaagccgtt tcgaaagcca agacggccaa ggccgctccc aaggcgaagg
      121 tttcgaaagc ggcccccaaa gccgcggcgc ggactcagcc gaaagccccg aaactttccg
      181 ggctggcctc tctgccggat gtggttcaga aggcggttaa attcctggac gaccggaagg
      241 cggaaaacat cgcggtgctc gacctgcgcg atctggccaa tatatcggaa ttttttgtga
      301 ttgtgaccgc cgcgaatgcg ccgcatctga aagcgctggg cgacggcctg cagcggctgt
      361 tcaagaacga gcagtatgaa ggataccgct tcgccgggac cggcgacagc ggctgggcca
      421 tcatcgacta tgacggcgta atggttcacg ttttcagcgc cgagatgcgc agtctatacg
      481 acctcgagaa gctctggaaa gatgcgaagc gcgtggcgat tgatttgaag cagttgtaac
      541 ccgcaggccg caggcggata cgagatagcg aacacaggct ggtttggagg acggtgtctt
      601 atgaaaagtt accgcaaaga actctggttc aagacgcctg tccggcgcgc ctttatcaat
      661 atcacgtccg aggttgccga cgctctgcgc gaaagcggca tccaggaagg actgtgtctc
      721 gtcaacgcca tgcacatcac cgccagcgtt ttcatcaatg acgacgaagg cggactgcat
      781 caggatttcg aaacgtggct tgaaacgctg gcgccggaaa agccgcacag tcaatatgcc
      841 cataacgggt ttgaggacaa cgccgacgct catctcaagc gccagatcat ggggcgcgaa
      901 gttgtgtgcg ccgttaccgg cgggaaactc gacttcggcc cgtgggagca gattttctac
      961 ggcgaattcg acggcctgcg cgacaagcgc gttctcgtga aaattatcgg cgagtaacat
     1021 ccgctaatct tcactgatcg gcactaatcc ggatttccca aattagtgtt cattagtgtg
     1081 aattagtggt taaactctga cccaatgaac cgtatcgcca aagtcgctgt cgacctctcg
     1141 ctggaccggg agtttgacta tctgattcct gaaccgctcc ggtcgctggt cgcgatcggc
     1201 tcgcgggtcg aggtgccgtt ccgctcgcgc acggtgaccg gctttgtggt cgggtttgcg
     1261 gatcaatccg cctttgacga tctcaagccg atcggaaaag tgctgggcga aacctccttg
     1321 gtcaccgaga cggtcatgga actggcgcgc tggatgtcgg cctattacct cgcgccgttt
     1381 gaagtctgtg tgcgcgccgt ccttcccgcc gttgtccgca acaaagaaag cggcggcaag
     1441 aagcagctga ctgtgtcgct ggttaatgtc gcagactcat catctcttcc gccgaaacag
     1501 caggcgctca ttgaatgcct ccgggaaaac ggcccgatgc ttctgtcgga gctgacggaa
     1561 gcggcaggcg tgacggccgc gccggtgaag gcgctggaga agaagggttt ggtgacggtt
     1621 tcgaatgagt cggtttaccg cgatccgcac gaaggtatcg agctgctgaa gacggccccg
     1681 ctggcgctga tgccgcagca ggcggatgcg ctgaagcaga ttgtcaccgc gatggctgaa
     1741 ccggaaccgc cggttttgct gctgcacggc gtgaccggtt ccggcaagac cgaggtctat
     1801 ttgcaggcga ttgccaaggc gctggagcag gagcacgggg ccattgtgct ggttccagaa
     1861 atcgcgctga cgccgcagac ggtggaccgt ttccgcgcgc gctttgccga tcatccgaag
     1921 caggtcgccg tgctgcacag ttcgctgtcg gacggcgagc gctacgacga gtggcaccgc
     1981 atccggtcgg gcgaggcgaa gattgtggtc ggcgcgcgct cggcgctgtt cgcgccggta
     2041 cacaatctcg ggctgatcgt tgtggatgaa gagcacgagc cgacctataa gcaggacgag
     2101 gcgccgcgtt acagcgcccg cgatatggcc gtattgcgcg ggcggctgga aaagtgcgcg
     2161 gtggtgctcg gctccgcgac gcccgcgctg gaatcctatg ccaacgcgaa gggcggcaaa
     2221 taccggtatg tcgatatgcc catccgcgtg gacgaccgcc ggatgccgcc gatccggatt
     2281 gtcgatatgc gcattgcggc gcagcaggaa ggcaagccgg ttctcttttc ccgtgagctg
     2341 gtcgaggcga tccgcacgcg gatcgaccgc gccgagcaga cgatgatttt cctgaaccgg
     2401 cgcggcttct cctcgtcgct gatctgtccg gactgtggct atgtggcgga atgcgagcat
     2461 tgcagcgtcg ggatgaccta tcacaaagcg cgggcgcggc tggtctgcca tatttgcggc
     2521 gaagagaagg cggtgccggt gcgctgcccg gagtgtaagt ccgatcagtt caagtatgcc
     2581 ggcgccggga cggaaaaaat cgaagaggtg atgacgaagc tgtttccgaa ggcgcgcatc
     2641 gagcggatgg attccgacac gatgcggcgc aagaacgcgc accgcgacat cctcagccgc
     2701 ttccggacgg gccggatcga tattctgatc ggcacgcaga tgatcgccaa agggctcgac
     2761 ttcccgaacg ttacgctggt cggcgtggtg aatcccgacc acgcgctgca tatggccgac
     2821 ttccgcgccg gcgagcgggt atttcagctg ctgacgcagg tggcgggccg cgccgggcgc
     2881 ggcgaggcgg cgggcgaagt gattgtgcaa acctacacgc cgcaccaccc ggcgattcag
     2941 gccgcgcggc gcatggattt tgacggcttc tgcgagcagg agctggcgtt ccgcaagcag
     3001 ctggactatc cgccgtatac ccatctgacc tgcatcacgc tgcgcggaaa aaccgaggcc
     3061 gaggtggagg ccgcggcgga aaaacttttt caggtgctgg aaaaatgtac ggccggcaag
     3121 gcggtgcttt ccccggcgat cccggcgccg attgcccgca tgcgcggcga ataccgctgg
     3181 cagattctgc tgcgggcggc gcggacgaag acgatgaatg attcgatccg cgcagccttc
     3241 gggctgatga aactgcc
//
LOCUS       DAOPZO010000169         3238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_40386_length_3238_cov_10.468740, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000169 DAOPZO010000000
VERSION     DAOPZO010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40386_length_3238_cov_10.468740"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..2183)
                     /locus_tag="PKI68_08670"
     CDS             complement(174..2183)
                     /locus_tag="PKI68_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53803.1"
                     /translation="MKRLLSVCILWCGLTVNAATIWLEDFSSYTSAGITGLGPTNYPG
                     SIANWSIDVCACAALNPGSGSAGDYFMAVATSGGRMEAVNVDGEAVWLSAVIRISDYT
                     NVLLSACAAETGSSASTNKYVKLFYRLDGGAETAFAVNPVNAGNWGSATAAQSNLYGS
                     TVQIVARINNPNTGDKSILDSVMVSGDRMVANLPPVLDAIGNREIAEPEPLTFTVTAG
                     DPANNDPVTLSAANLPPGAVFTNGVFTWTGAAPAGTYAVTFYATDKDGTVSETITITV
                     TPAAAGNSRIAGNFYGWSGDTIFKLETGRFWQQCAAGTKAISPALYRPYVTVTNVYGQ
                     RRMVVTNVTGYAVVAPLSVAESAVTNFFSGLHCQNIYQLADGTVWRQISFENISSAAS
                     PVTAWRWIKNGRQMLRFLDRSNAVIGTCTAEADTPPAEAPVVSKIDGYFYGFGHGNIY
                     RLADGSWWKQISFERSGVTRLNPKVLVRNENGNSYLELPDEGRCITADKLNVQLESTA
                     AGTFTGMRYGNLYRLANGESWLQISFENVRTNIAGPEVMLWTDRAGTGLLVRSGGATI
                     GTCTVIDPAPDDDHDGLSNAAEILADSDPLNAQSRFELRQTDHCILSWDPVEARVYTI
                     EWTPSLTESFQPLATGLVRPQNSWTDTVHSAGSKGYYRITVRFAE"
     gene            complement(2180..>3238)
                     /locus_tag="PKI68_08675"
     CDS             complement(2180..>3238)
                     /locus_tag="PKI68_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53804.1"
                     /translation="QLLGRYNRKALDERSRQVEIETKFIEVAQGALDELGFDWTVGTP
                     GENITSDTWTMPGGQKLFTDSLRTGQEAFGKSVDAGGRAASFNTYANATTDPAGTGMI
                     GTAGELLVQKVKGDLKIDLLIRALERTAGSDLLSAPKILTKSGETASIHVGEVHWFPS
                     AYDVKIERYAPPSLVPLDYLEERTGVMLKVTPELDPENGTINMKLSPEIRELAGFDEQ
                     HVSTIWPEYGSESLDMNSDTPASSSLIDFLTRREESRSINADRLIARRPVFKTRKVDT
                     AVTIEDGSTIAMGGLIKEQLETFRDSVPLLGKIPILGRLFRSEGERNVKRNLLIFVTA
                     NQVDASGHRNNMRQAAEL"
BASE COUNT          626 a          915 c          953 g          744 t
ORIGIN      
        1 atggtcattc cgaccgccgc gagcgccttc tatctgaagc tgctctccgg tctgactcag
       61 agcttcgcga ccaaagaagc gcgcgactcg ctgctcaaag ccaagacgca ggaagaactc
      121 tggaaggcgc tggttaaagc caccaaaaag atgatccagt aattccggag cctttattcc
      181 gcaaagcgga cggtgatgcg gtagtaccct ttggatccgg cactgtgaac ggtgtcggtc
      241 cagctgttct gcggccggac gagtccggtc gccagaggtt gaaaactttc cgtcagcgat
      301 ggcgtccatt ctatcgtgta aacgcgggct tcgaccggat cccagctcag tatgcaatga
      361 tcggtttgac gaagttcaaa ccgggactgc gcattcagcg ggtcggaatc ggccaatatc
      421 tctgccgcat tgcttaagcc gtcatggtca tcgtccggcg ccggatcaat gactgtgcag
      481 gttccgatcg tggcgccgcc gctgcgcacc agcagaccgg ttccggcccg atccgtccag
      541 agcatgacct caggtccggc gatgttggtt ctgacgtttt caaaactgat ctgcagccag
      601 ctctcgccgt tagccagccg gtacagattg ccatagcgca tcccggtgaa cgtaccggcg
      661 gcagtgcttt ccagttgaac attcagtttg tcagccgtaa tgcaccggcc ttcgtcaggc
      721 agttcgagat aggaatttcc gttttcattc cgcaccaaaa cttttggatt cagccgtgtg
      781 accccggacc gctcgaatga aatctgtttc caccacgatc cgtcagcgag gcggtaaata
      841 tttccgtgtc cgaaaccgta aaaatatccg tcgattttgc tgacaaccgg cgcttctgcc
      901 ggcggcgtgt ctgcttctgc tgtgcaggtt ccgataacgg cgttgctgcg gtcgaggaac
      961 cgcagcattt gccggccgtt tttaatccag cgccaggcgg tgacgggcga agcggcactg
     1021 gaaatatttt cgaacgaaat ctgcctccat acggttccgt ccgcgagctg atagatgttc
     1081 tggcagtgca gtccggaaaa gaagttggtg acggcgcttt ctgcaacgct cagcggagcc
     1141 acaactgcat agccggtgac gttggtcaca accattcgcc gttggccgta aacattcgtg
     1201 accgtcacgt acggacggta cagcgcggga ctgatggcct tggtgccggc ggcgcattgc
     1261 tgccagaacc ggccggtttc gagtttaaag atcgtgtcgc cgctccagcc gtaaaaattt
     1321 ccggcgatgc gcgaatttcc ggcggctgcg ggggtgaccg tgatggtgat cgtttcgctg
     1381 accgtgccgt ctttatcggt tgcataaaat gtgacggcat acgtaccggc cggtgcggcg
     1441 ccggtccagg tgaacacgcc gtttgtaaag acagcgccgg gcggcagatt ggccgcactc
     1501 aatgtaacgg ggtcgttgtt cgcggggtcg ccggcagtaa cggtgaatgt gagcggctcc
     1561 ggctcggcaa tttcccggtt gccgatggcg tccagtaccg gcggcagatt ggcgaccatg
     1621 cggtcgcccg aaaccatgac gctgtccagt atggatttat cgccggtgtt cgggttgttt
     1681 attcgtgcga cgatttgaac ggttgatcca tacagattgc tttgcgccgc cgtcgccgaa
     1741 ccccagttgc cggcattcac cggattaacc gcaaacgctg tttctgcgcc gccatccagc
     1801 cggtaaaaaa gcttcacata tttattggtg ctggcggaag agccggtttc ggcggcgcag
     1861 gcggaaagta aaacgttggt atagtcgctg atgcgaatga cggccgataa ccaaacagct
     1921 tcgccgtcta cattaactgc ctccatgcgt ccgccgctgg tggcgacggc cataaaataa
     1981 tcgccggcgc tgccgcttcc cggattcagc gcggcgcagg cgcaaacatc aatgctccag
     2041 tttgcaatgc tgccggggta gtttgtcggc cccagcccgg tgatgccggc gctggtgtaa
     2101 ctgctgaaat cttccagcca tatggttgcg gcattaaccg tcaggccgca ccagagaata
     2161 caaaccgaca gaagccgttt catagttctg cagcttgccg catattgttt cgatggccgg
     2221 aagcgtccac ctggtttgcg gtcacgaaaa tcagcaggtt gcgtttcacg ttacgttcac
     2281 cttccgagcg gaagaggcgg ccgagaatcg gaattttccc aaggagcggg acgctgtccc
     2341 tgaaggtttc gagctgttcc ttaatcagtc cgcccatcgc gatggtgctg ccgtcttcga
     2401 tcgtcacggc cgtatcgacc ttgcgggttt taaataccgg acggcgggcg atcagccggt
     2461 cggcattgat cgagcggctt tcttcgcggc gggttagaaa gtcgatcagg ctgctgcttg
     2521 ccggagtatc gctgttcatg tccagcgatt cactgccgta ttccggccag atggtgctga
     2581 catgctgttc gtcaaatccg gccagctcgc ggatttccgg cgaaagtttc atgttgatgg
     2641 ttccgttttc gggatcgagt tcgggggtga ctttgagcat gacgccggtg cgctcctcca
     2701 gataatcaag cgggacaaga ctgggcgggg catagcgctc gatctttaca tcgtatgctg
     2761 acggaaacca gtgcacctcg ccgacgtgaa tgctggcggt ttcgccgctt ttggtgagaa
     2821 tcttcggcgc ggaaagcaga tcggatccgg cggtgcgttc cagtgcgcga atgagtaaat
     2881 cgatcttcaa gtcgcctttt actttctgta ccagaagttc gcctgccgtt ccgatcatgc
     2941 cggtgccggc cggatcggtt gttgcgtttg cataggtgtt aaacgacgcg gcccgcccgc
     3001 cggcatcaac tgatttccca aacgcctcct gcccggtgcg caggctgtcg gtgaaaagct
     3061 tctgcccgcc gggcatcgtc caggtgtcgg aggtgatatt ttcgccggga gttccgaccg
     3121 tccagtcgaa ccccagttca tcaagcgcgc cctgcgcgac ttcgataaat ttggtttcaa
     3181 tttcgacctg ccgtgaccgc tcatcaagcg ccttgcggtt ataccgtccg agcagctg
//
LOCUS       DAOPZO010000170         3210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_40905_length_3210_cov_11.017433, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000170 DAOPZO010000000
VERSION     DAOPZO010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40905_length_3210_cov_11.017433"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..80
                     /locus_tag="PKI68_08680"
     tRNA            4..80
                     /locus_tag="PKI68_08680"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38..40,aa:Ile,seq:cat)
     gene            complement(59..823)
                     /locus_tag="PKI68_08685"
     CDS             complement(59..823)
                     /locus_tag="PKI68_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNX53805.1"
                     /translation="MNPLENIRIVLVEPKYGGNIGSVCRAMMNMGVTDLALVGRPDQF
                     DLEEARKLAYNADGMIDRLAIFKTLEEAVADCTVVAGTSARTGFYRDHAYLPRDLAPV
                     VLDSAAHHKVALVFGREDKGLFNEEIALCTHIINIPSSDLYRSLNLSHAVMVCCYEIF
                     LLANNPSYAPPEECSPEADGRMRERLYEAWSRMMLATGFCENEKLDHMMLGLRRIFSR
                     GKLTVNDTKILLGLAKQSCWVAEELNRKKSGGPART"
     gene            933..2375
                     /locus_tag="PKI68_08690"
     CDS             933..2375
                     /locus_tag="PKI68_08690"
                     /inference="COORDINATES: protein motif:HMM:NF006088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNX53806.1"
                     /translation="MADFPTTAPRPLCVDLDGTLVKTDTFAQALLLLVRTRPAALFSI
                     PGWAAGGLAAFKRRIAREIRLDPAALPYHAELLAFLKSEQAKGRPLILATASDELPAR
                     AVADHLGLFSDVIASDGVTNLKAGRKRAALVARFGEKGFDYAGNATDDLAVWESAAHM
                     IAVNPCATVRRALNSRPAQLFENRPAKLNVWCKALRVQQWAKNILIFLPMLLAHRLST
                     PELYIKAALAFFAFSFAASAIYILNDLLDLHADQHHPRKKNRPLAAGNLSLLAAAIAA
                     PLLVLTALCTAQLLPAQFTAILFVYLLITTLYSWRLKQLALLDVVTLAGLYAIRILAG
                     TAAYGVETSGWLIAFSLCIFFSLALVKRYAELREAVAAQPAKIGARGRGYHARHLPWL
                     FRCGIAGGVLSVGVLACYITSPKVLEFYSRPAALWLLCPLLLYWIIRVWRLAAGGRLS
                     DDPLEFAARDRQTWLIGAISAAVLILGSIL"
     gene            2372..>3210
                     /locus_tag="PKI68_08695"
     CDS             2372..>3210
                     /locus_tag="PKI68_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNX53807.1"
                     /translation="MKPTLVIMAAGIGSRYGGLKQIDPVGPSGEIVLDYSVYDAIQTG
                     FGKVVFIIRPDIEEAFKEVIGHKLDGKIQVEYVYQTLEKLPDGGSVPAGRTKPWGTGQ
                     AVLMAAPVVHEPFAVINADDFYGRDSFRAVAEQLQKTDVNSTDFCMAGFYIKNTLSEH
                     GGVARGYCDVENGKLKHIVERFNIERKDDGVIHYAGGTMNDNDMVSMNTWGFTPKLFD
                     FLERGFEAFLAQKGNELKSEFYLLDVVDGMISRGEAEVTVSPTAEKWMGVTYADDKPQ
                     VMAG"
BASE COUNT          609 a         1066 c          926 g          609 t
ORIGIN      
        1 ttcgggcccg tagctcagtc ggtcagagca ggagactcat aatctcttgg tcctcggttc
       61 aagtccgagc gggcccacca ctctttttcc ggttcaactc ctccgccacc cagcacgact
      121 gtttggccag gccgagcagg attttggtat cgttgacggt gagtttgccg cggctgaaga
      181 tgcgccgcag gccgagcatc atgtgatcga gcttttcgtt ttcgcagaag ccggtggcga
      241 gcatcatgcg gctccacgcc tcgtacaggc gttcgcgcat gcggccgtcg gcctccggcg
      301 agcactcttc cggcggggcg tacgacgggt tgttggccag cagaaaaatt tcgtagcagc
      361 agaccatgac ggcgtgcgag aggttgagcg agcggtagag atcgctcgac ggaatgttga
      421 tgatgtgggt gcagagggcg atctcttcgt tgaaaagccc tttatcttcc cgcccgaaca
      481 ccagcgccac tttgtggtgc gcggcggaat cgagcacgac gggcgccaga tcgcgcggca
      541 gataggcgtg gtcgcggtaa aacccggtgc gggcgctggt gccggccacg acggtgcagt
      601 cggccacggc ctcttccagc gtcttgaaaa tggcgagccg gtcgatcatg ccgtcggcgt
      661 tataggccag cttgcgggcc tcttccaggt cgaactggtc cggccggccg accagcgcga
      721 gatcggtcac ccccatattc atcatggcgc ggcagaccga gccgatgttg cccccgtatt
      781 tgggttcgac cagcacaatg cggatatttt cgagcgggtt cataggtggg cgcgatttga
      841 acagatccgg gggtgcgtcg acaatgcaaa agaccggaga gaaagcagaa cggggttgaa
      901 cttgccccgg ttcttctaaa tactcccgcg aaatggctga ctttcccaca actgcgccgc
      961 gtccgctctg cgtcgacctc gatggaacgc tggtcaaaac cgacaccttt gcgcaggcgc
     1021 tgctgctgct cgtccgcacc cgtccggccg cgcttttttc cattcccggc tgggccgccg
     1081 gcggactggc cgccttcaaa cggcgcatcg cccgggaaat ccggctcgat ccggccgcgc
     1141 ttccgtacca tgccgaactg ctcgcttttc tgaaaagcga acaggccaaa ggccgcccgc
     1201 tgattctggc tacggcttcc gacgaactgc cggcgcgcgc cgtagccgat cacctcggcc
     1261 tcttttcaga cgtcatcgcc agcgacggcg tgaccaacct caaagccggc cgcaaacgcg
     1321 ccgcgctcgt cgcgcgcttc ggcgaaaagg gcttcgacta tgccggtaac gccaccgacg
     1381 acctcgccgt ctgggaatcg gcggcccata tgattgccgt caatccctgt gcgacggtgc
     1441 gccgcgcgct caacagccgc ccggcgcagc tttttgaaaa ccgcccggcg aaactcaacg
     1501 tctggtgcaa agccctgcgc gttcagcagt gggccaaaaa cattctcatc tttctgccga
     1561 tgctgctggc gcaccggctc agcacgccgg agctgtacat caaagccgcg ctggccttct
     1621 tcgccttcag cttcgccgcg tcggccatct atattctcaa cgacctgctc gacctgcacg
     1681 ccgaccagca ccatccgcgc aaaaaaaacc ggccgctggc ggcgggcaac ctcagcctgc
     1741 tcgccgccgc catcgccgcg ccgctgctgg tgctgacggc gctgtgcacc gcgcagctgc
     1801 ttccggcgca attcaccgcc atcctgtttg tttatctact gattaccacc ctctattcct
     1861 ggcggctgaa acagctcgcg ctgctcgacg tcgtcacact cgccggactc tacgccatcc
     1921 gcatcctcgc cggtaccgcc gcctacggcg tcgaaacctc cggctggctg attgcctttt
     1981 cgctctgcat cttcttcagc ctcgcgctgg tcaaacgcta cgccgaactg cgcgaagccg
     2041 ttgcggcgca gccggcaaaa atcggcgcgc gcggtcgcgg ctatcacgcc cggcacctgc
     2101 cgtggctgtt ccgctgcgga atcgcgggcg gcgtgctgtc cgtcggcgtg ctggcgtgct
     2161 acatcaccag cccgaaagtc ctcgaatttt acagccgccc cgctgcgctc tggctgctct
     2221 gcccgctgct gctctactgg atcatccgcg tctggcggct cgccgccggc ggacgccttt
     2281 cggatgatcc gctggaattt gccgcgcgcg accggcagac gtggctgatc ggcgccatca
     2341 gcgcagccgt tttaatctta gggagtattt tatgaaaccg accctcgtca tcatggccgc
     2401 cggaatcggc agccgctacg gcggactcaa acagatcgac cccgtcggcc cgtccggcga
     2461 aatcgtcctc gactattccg tgtacgacgc catccagacg ggcttcggca aagtcgtctt
     2521 catcatccgc ccggatatcg aagaagcctt caaagaagtc atcggccaca aactcgacgg
     2581 aaaaattcag gtcgaatacg tataccagac gctggaaaaa cttcctgacg gagggtctgt
     2641 tccggccggc cgcaccaaac cgtggggcac cggacaggcc gtactgatgg ccgctcccgt
     2701 ggttcacgaa cccttcgccg tcatcaacgc cgacgatttt tacgggcgcg actccttccg
     2761 ggccgtggct gaacagctgc agaaaacgga tgtgaacagc acggatttct gcatggccgg
     2821 cttctacatc aaaaacaccc tctccgaaca cggcggcgtc gcgcgcggct actgcgacgt
     2881 cgaaaacgga aaactcaaac acatcgtcga acgcttcaac atcgagcgca aagacgacgg
     2941 cgtgatccac tacgccggcg gaaccatgaa cgacaacgac atggtttcca tgaacacctg
     3001 gggcttcact ccgaaactgt tcgactttct cgaacgcggc ttcgaagcgt tcctcgcgca
     3061 aaaaggaaac gaactcaaaa gcgaattcta cctgctggac gtggtcgacg gcatgatcag
     3121 ccgtggcgaa gccgaagtca ccgtatcgcc gaccgctgaa aaatggatgg gcgtcaccta
     3181 cgccgacgac aaaccgcagg tcatggccgg
//
LOCUS       DAOPZO010000171         3112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_42798_length_3112_cov_8.879293, whole genome shotgun sequence.
ACCESSION   DAOPZO010000171 DAOPZO010000000
VERSION     DAOPZO010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42798_length_3112_cov_8.879293"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3044)
                     /gene="nifJ"
                     /locus_tag="PKI68_08700"
     CDS             complement(<1..3044)
                     /gene="nifJ"
                     /locus_tag="PKI68_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNX53808.1"
                     /translation="MSKKMVTIDGNTAAAHVAYAFSEIAAIYPITPSSNMGEYADAWA
                     AQGRKNIFGSVVSVTEMQSEAGAAGAVHGALSTGSLTTTFTASQGLLLMIPNMYKIAG
                     EMLPAVFHVSARALAAQSLSIFGDHSDVMSVRNTGWAMLAANGIQETMDLAIVSHLST
                     LKGQVPFVNFFDGFRTSHEVQKVEEIGYDTIKELLEPEYVERFRKRGLRPESPVLKVA
                     AQNPDVYFQGRETCNKYYDALPEIVQDYMNKLAAKIGRQYHLFDYAGAPDADKVIIAM
                     GSACDTIEQTINHLNAKGEKLGLVKVRLFRPFSIKAFVGCLPKTVKKIAVLDRTKEPG
                     NIGEPLFLDVTAALENKGIKIIGGRYGLSSKEFTPAMVKAVYDHLDGAAFHGFTVGIN
                     DDVTNLSLKVGKEMNIEAKDVVNCMFWGLGSDGTVGANKNSIKIIGDNTDMTAQGYFV
                     YDSKKSYGITVSHLRFGKSSVNYPYLIQHADFVACHNPAYIGRYDMLGCIKEGGTFLL
                     NSEIPSDEVFNHLTREMQEIIIQRKIKFYNINALKISVEAGLGARINTVMQTAFFKLS
                     GVLEQDKAIELIKNAIKKSFAKKGEDIIKMNWACVDKACAALEQVKIPATITKSFVFP
                     TLINEAPGCFAKDVMEPVLLLKGDDVPVSKMSFDGVLPTGTSALEKRGIAPRVPHWVK
                     ENCIQCNQCVMACPHAVVRAKQIDPKDLTKKPEGFCTLKSNTKNDKNLEYKIQVYIED
                     CTGCGVCVETCPAKTKALEFKTIEEEREAGERKNAEFFEALPDNVLDGAADSTLKGLQ
                     FKKPLFEFSGACGGCGETPYVKLVSQICGEHMIVANATGCSSIYGGTFPAIPYCKSKE
                     GRGPAWGNSLFEDNAEYGLGMRLAVDNNRNMLKIKTAKAIELGVDKDLAAAMTKALEA
                     GNTANDAAVAAQNAVKMLLPGALKAASGELKEVLTKISELSDFFVDKSVWIFGGDGWA
                     YDIGYGGLDHVVASGKNVNILVLDTEVYSNTGGQMSKSTPIGA"
BASE COUNT          592 a          837 c          911 g          772 t
ORIGIN      
        1 gcgccgatcg gggtggactt ggacatctgg ccgccggtgt tggagtacac ttcggtatcg
       61 agtaccagaa tgttgacgtt cttgccggaa gcaaccacat ggtcgagtcc gccgtaaccg
      121 atgtcatagg cccagccgtc accgccgaaa atccagacgc tcttatcgac aaagaagtct
      181 gaaagttcgc tgattttcgt caggacttcc ttcagttcgc ctgaagcggc tttgagcgcg
      241 cccggcagga gcatcttgac ggcgttctgc gcggccacag ccgcatcgtt ggcggtattg
      301 cccgcttcga gcgctttggt catcgcggcg gcgaggtctt tatcgacacc cagttcgatc
      361 gccttcgcgg ttttgatctt gagcatgttg cggttgttat ctaccgccag acgcatgccg
      421 agaccgtatt ccgcgttgtc ttcgaacagc gagttgcccc aggccggacc gcggccttct
      481 ttgctcttgc agtaggggat cgccgggaat gttccgccgt agatggagga acagccggtg
      541 gcgttggcaa cgatcatgtg ttcgccgcag atctggctga ccagtttgac gtacggagtt
      601 tcgccgcaac cgccgcaggc accggagaac tcaaacagcg gcttcttgaa ctgaaggcct
      661 ttgagggttg aatcagccgc gccgtcgaga acgttgtccg gaagcgcttc gaagaactcg
      721 gcgttcttgc gttcgccggc ttcgcgttct tcttcaatcg tcttgaattc gagcgctttg
      781 gttttggccg ggcaggtttc aacgcagacg ccgcaaccgg tgcagtcttc gatgtaaacc
      841 tgaatcttgt attccagatt cttatcgttc ttggtgttcg acttgagcgt acagaaacct
      901 tccggcttct tcgtcagatc tttcggatca atctgtttgg cgcggacaac tgcgtgcggg
      961 caggccatta cgcactgatt gcactgaatg cagttttctt tgacccagtg cgggacgcgc
     1021 ggagcaatac cgcgtttttc cagcgcgctg gtgccggtcg gcaggacgcc gtcgaacgac
     1081 attttggaaa ccggcacatc gtcgcctttc agcagaagaa ccggttccat gacatccttg
     1141 gcaaagcagc cgggggcttc gttgatcagg gtcgggaaaa cgaacgactt ggtgatcgtc
     1201 gccggaatct tgacctgttc cagtgccgcg caggctttgt ctacgcaggc ccagttcatc
     1261 ttgatgatgt cttcaccctt cttggcgaag ctcttcttga tggcgttttt gatcagctcg
     1321 atcgccttgt cctgttccag cacgccggaa agtttgaaga aggcggtctg catgacggtg
     1381 ttgatgcgtg cgccgagtcc ggcttccacg gaaatcttca gagcgttgat gttgtagaac
     1441 ttgatcttgc gctggatgat gatttcctgc atttcgcggg tcaggtggtt gaacacttcg
     1501 tcgctcggga tttccgagtt cagcaggaat gttccgcctt ccttgatgca gccgagcatg
     1561 tcataacggc cgatgtaggc cgggttgtgg caggccacaa agtcggcatg ctgaatcagg
     1621 taggggtagt tcacgctgct cttgccgaag cgcaggtggg aaaccgtgat accgtaggat
     1681 ttcttcgaat cgtatacgaa gtagccctgg gcggtcatgt cggtgttgtc gccgatgatc
     1741 ttgatcgagt tcttgttcgc gccgacggtt ccgtctgagc cgagacccca gaacatacag
     1801 ttcacgacat ctttggcttc gatgttcatt tccttgccga ctttgagcga caggttggtt
     1861 acgtcatcgt taataccgac cgtgaagccg tggaaggccg cgccgtcgag atggtcgtag
     1921 acggctttca ccattgccgg cgtgaattcc ttggaggaaa gaccgtaacg gccgccgatg
     1981 atcttgatgc ccttgttttc gagagcggcg gtaacgtcga ggaacagcgg ctcgccgatg
     2041 ttgcccggtt ctttcgtgcg gtcgagtaca gcaatcttct tgaccgtttt cggcaggcag
     2101 ccgacgaagg ctttgatcga gaagggacgg aagaggcgaa ccttaacgag accgagcttt
     2161 tcgcccttgg cgttcagatg gttgatggtc tgttcgatcg tatcgcaggc cgagcccatc
     2221 gcgataatca ctttatcggc atccggtgcg ccggcgtagt cgaacaggtg gtactggcgg
     2281 ccgatcttcg cggccagctt gttcatgtag tcctgcacga tttccggcag agcatcgtag
     2341 tacttattgc aggtttcccg gccctggaaa taaacgtccg ggttctgcgc ggcaactttc
     2401 agcaccggag attcagggcg cagaccgcgc ttgcggaaac gttcaacata ttccggttcc
     2461 agcagttcct tgatggtgtc gtaaccgatt tcttcaacct tctgcacttc gtgcgaggtg
     2521 cggaaaccgt cgaagaagtt tacaaacgga acctggccct tcagcgtcga gaggtggctg
     2581 acgatggcga ggtccatggt ttcctgaatg ccgttggcgg ccagcatcgc ccagccggtg
     2641 ttgcggacgc tcatcacgtc ggaatggtcg ccgaaaatgg agagtgactg tgcagccagc
     2701 gcgcgggcgg aaacgtggaa aaccgccggc agcatttcac ctgcgatctt atacatatta
     2761 ggaatcatca gcagcagacc ctgcgatgcg gtaaaggtgg tcgtcagcga accggtgctc
     2821 agcgcaccgt gtacggctcc ggcggcgccg gcttcggact gcatttccgt tacgctcact
     2881 acagagccga aaatattttt acgtccctgg gcggcccagg cgtctgcgta ttcacccatg
     2941 ttggatgaag gggtgatcgg gtaaattgcc gcgatttcgc tgaaggcata cgccacatgc
     3001 gctgccgcgg tatttccgtc aatggtaacc attttcttgc tcatacctgc ttctttctcc
     3061 tgaggattcg ggtttctatt aaaattccgt gaaaactctc atctctggct ca
//
LOCUS       DAOPZO010000172         3070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_43670_length_3070_cov_7.314428, whole genome shotgun sequence.
ACCESSION   DAOPZO010000172 DAOPZO010000000
VERSION     DAOPZO010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43670_length_3070_cov_7.314428"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..658)
                     /locus_tag="PKI68_08705"
     CDS             complement(71..658)
                     /locus_tag="PKI68_08705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobile mystery protein B"
                     /protein_id="HNX53809.1"
                     /translation="MINFDYPDGATPLDPDEVEGLKLTHITSRKELDRWEQDNILEAL
                     SWLESARPKDILNEQFIRKLHRKMFGNVWKWAGKFRLSDKNIGGPWHQVPVELKNLCD
                     DVTAWIDAGQMPPDEMAARLHHRLVSIHPFSNGNGRHARLMADLLLENVLNQQKFTWG
                     SRNLVKAGDARSAYIAALRAADRFDIKPLIEFARS"
     gene            complement(655..1113)
                     /locus_tag="PKI68_08710"
     CDS             complement(655..1113)
                     /locus_tag="PKI68_08710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobile mystery protein A"
                     /protein_id="HNX53810.1"
                     /translation="MKNSKLIREQLGANLSKFRPLLEIQAPQKGWIRAIRDALGMSAK
                     QLAERTGMAQQRIAVVEKGEFTGAVTLKTMRRIAEGLDCVFVYGFVPKTDLETTLRKQ
                     AEQIAAKRLSQAAHTMGLENQSLSEQENRKVFEETVEKLIENPTALWNKI"
     assembly_gap    1293..1392
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1393..2324)
                     /locus_tag="PKI68_08715"
     CDS             complement(<1393..2324)
                     /locus_tag="PKI68_08715"
                     /inference="COORDINATES: protein motif:HMM:NF021508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HNX53811.1"
                     /translation="MTTRNLVLLFDGTWNKADTGIDDDQTNVEKLYRLLPDSYVKNVH
                     YEEGVGIKSEEEILGGAFGAGIDERILGAYRFLQSRYTDQGFDNRLFIFGFSRGAYTA
                     RTFAQLLDYCGVPTAKGNCQTAWNCYYRQDRQAAEKLKKSGNFFNVGINMLGVWDTVK
                     STLDPDRQDTTLYPSVKAAYHAMAIDEKRRNFPILRFKEDPRITQVWFAGVHSDVGSG
                     YAGNESQLSDIALAWMVAAARQNGLEFVADAADYMDPNPTGKIHDSYTGFWKALGENV
                     RVIGANDSVHSSVKARLNGNVGYHPVNLPANPHYV"
     gene            complement(2321..2695)
                     /locus_tag="PKI68_08720"
     CDS             complement(2321..2695)
                     /locus_tag="PKI68_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53812.1"
                     /translation="MRYIELKVNRLVPVGFAMLIAISVLSGCMNIPVEGDCPKTPDSK
                     YVRETVHGSYYGFNWDDDSRQVRKADNGLGLAKVEYRSNFFYALVSVVTLGAYAPVDV
                     DCWVESPAVIHRLPKKTGGASE"
BASE COUNT          643 a          821 c          781 g          725 t
ORIGIN      
        1 tttccagaga caggcaggat gtataaccgt tttccgcacc gcccgccagc ccttgctgtt
       61 ggaaagttct ctatgaccgg gcaaactcga taagcggttt aatgtcaaaa cgatctgcgg
      121 cgcgcagtgc ggcgatatat gctgagcggg catcgccggc tttcacaaga ttcctgcttc
      181 cccaggtgaa cttttgctgg ttcagcacat tctccagaag cagatctgcc atcagccgcg
      241 catgccttcc gttgccgttt gaaaacgggt gaatggatac cagtcggtgg tgcaggcgcg
      301 cggccatttc atcgggcggc atttgtccgg cgtcgatcca tgccgtgaca tcgtcgcaaa
      361 ggtttttcag ttcgacggga acctgatgcc acgggccgcc gatattttta tcactgagcc
      421 ggaattttcc ggcccacttc cagacgttgc cgaacatttt ccggtgcagt ttgcgaatga
      481 actgttcatt cagaatatct ttcgggcggg cggattccag ccaactcagc gcttccagaa
      541 tattatcctg ttcccagcgg tcgagttctt ttcgcgaggt gatatgtgtc agcttgagac
      601 cttcaacttc atcgggatca agcggtgtgg ctccgtccgg ataatcgaaa ttaatcatat
      661 tttgttccac agtgcggttg gattttcgat cagcttctcg acggtttctt cgaagacctt
      721 ccggttttcc tgttcgctga gcgactggtt ctcaagcccc atcgtatggg cggcctgcga
      781 aagacgcttg gcggcaattt gctcggcctg ttttcttaag gtcgtctcca gatcggtttt
      841 aggaacaaag ccgtaaacga aaacacagtc cagaccttcg gcgatccggc gcatggtttt
      901 cagtgtgacc gcgccggtga attcaccctt ttcgacgacc gcaatccgct gctgagccat
      961 tcctgtgcgt tccgccagct gttttgcgct catgcccaag gcatcgcgaa ttgcgcgaat
     1021 ccagcctttt tgaggtgctt gaatctctaa aagcggtctg aatttagata aattcgctcc
     1081 aagctgctct cggatgagtt tgctgttttt cataacgaat cctttcgacc ggactattta
     1141 caacataaaa ggtgtattta tgtcgataaa aaacaatata taaattgtat aaattaaaaa
     1201 atatacatct taaaaaatgt atataatcgg ccctcagccc cgaataattt ggcagaatgg
     1261 ataaccgttt ccctcaccgc ccgccagccc tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnacataatg cgggttggcc ggaagattga cggggtgata gccgacgttg
     1441 ccgttcaggc gggctttaac cgacgagtgc acgctgtcgt tggcgccgat gacgcggacg
     1501 ttttcgccga gcgccttcca gaagcccgtg taggaatcgt ggatttttcc ggtcggattg
     1561 gggtccatat aatccgcagc atccgccaca aactccagcc cgttctgccg ggcggccgca
     1621 accatccagg ccagcgcaat gtcggagagc tgcgattcgt tgccggcgta gccgctgccg
     1681 acatcgctgt gcacaccggc gaaccacacc tgcgtaatgc gcgggtcttc cttgaagcgc
     1741 aggatcggaa aattgcgccg cttctcgtca atcgccatcg cgtgataggc cgccttgacg
     1801 ctcgggtaaa gggtggtgtc ctgccggtcc ggatccagcg tcgatttgac cgtgtcccag
     1861 acgcccaaca tgttgatgcc gacattgaag aagttgccgg atttcttgag tttttctgcg
     1921 gcctggcggt cctggcggta ataacaattc caggcggttt ggcagttgcc cttggcggtc
     1981 ggcaccccgc agtagtcgag cagctgggca aacgtgcgcg cggtatacgc gccgcggctg
     2041 aacccgaaaa tgaacagccg gttgtcgaac ccttgatcgg tgtagcggct ttgcagaaag
     2101 cggtaagcgc ccagaatccg ctcgtcgatt cccgcaccga aagctccgcc gagaatttct
     2161 tcttcgcttt taatgcccac gccttcttcg tagtggacat ttttcacata tgagtcgggc
     2221 agtagccggt agagtttttc cacattggtt tggtcgtcgt cgatgccggt gtcggccttg
     2281 ttccatgtcc cgtcaaaaag cagcacgagg ttccgcgttg tcattctgaa gctcctccgg
     2341 ttttcttagg caggcgatga atgacggccg gcgactcaac ccagcagtcc acatccaccg
     2401 gagcgtaggc tcccaacgtc accacactaa ccagcgcgta gaagaaattg ctgcggtatt
     2461 caaccttggc cagaccgagc ccgttgtccg ccttgcggac ctgtcgcgaa tcgtcatccc
     2521 agttgaagcc gtagtaggag ccgtgcacgg tttcccggac atatttggag tccggcgttt
     2581 tcgggcagtc gccctccacg ggaatattca tacagccgct cagcacgctt atcgcgatga
     2641 gcatcgcgaa tccgaccgga accagacggt taactttcag ctcaatatat cgcatcgtcc
     2701 ttctcctttc cggaggcgac gtatcgcccc gtgtaaaacg attaaagaat aggtaaaacc
     2761 tgcataaacg taacgatgca aagaatgggt gtcaaagaaa agagccggat tgatgaagca
     2821 aagcgaacta gaacatgtaa cgatgtaaga ggcgcaaaag gtaaagccgg aacttagggc
     2881 gtattcgccc gtagatccgg cttcctggcg ttaatagccg gatccacggc ttcgcctgag
     2941 ttccggctat accgacttct caatcgtcaa ccgctctatt cagagctgct ccgcaatcca
     3001 cagcatcttt gaagcggcag gatgtataac cgttttccgc accgcccgcc agcccttgct
     3061 gttggaaagt
//
LOCUS       DAOPZO010000173         3039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_44325_length_3039_cov_6.805295, whole genome shotgun sequence.
ACCESSION   DAOPZO010000173 DAOPZO010000000
VERSION     DAOPZO010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44325_length_3039_cov_6.805295"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1574
                     /gene="secF"
                     /locus_tag="PKI68_08725"
     CDS             <1..1574
                     /gene="secF"
                     /locus_tag="PKI68_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNX53813.1"
                     /translation="GSFTVDECTRLVNVLRAGALPAPVTIVEERTVDPSLGKDSIDSG
                     MRACIYGAIAVVVFMAGYYLLAGVIADLSLLMLMLMLPFGMWFSSGILSLFSAGAGAS
                     AGLPVLTLPGIAGIVLTLGMAVDANVLIYERIREELALGKSVVSSISAGYDKAFATIF
                     DSNLTTLVAAVIMFWQGSGSIRGFAVTLTAGILVSMLTALVFTRLLLDTLAAKTKLKT
                     IKMFSFFPKTKIDFMGKRYIAIGLSWAIIIISWIILFHKGEKNFGVDFTGGTSITFAF
                     DQKQPAEAVRAVLEKAGIKDAAIQYHGELGVAEGAKANETLEVKVSYENGNTAIDAVK
                     TAFAAQGYKDIANDSVGPQIGQELKRKGAMSMILALVGITIYIAFRFEFGFAIGAIVA
                     TVHDVLITIGLYCLFGRELNLTIVAALLTIIGFSVNDTIVVFDRIRENIKLLHGTKAS
                     YAEISNLSINQTLSRTVLTSLTVVLTVISLLIFGGGAIFDFALTLLIGFTVGTYSSIY
                     VATPVALLWHKEKKG"
     gene            1637..>3039
                     /gene="recJ"
                     /locus_tag="PKI68_08730"
     CDS             1637..>3039
                     /gene="recJ"
                     /locus_tag="PKI68_08730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNX53814.1"
                     /translation="MAKQWKFKPCDETLAEHIFRKLGNMPRPLTALLVQRGCKTAAEA
                     ELFMNPALSTLRDPFELPDMDKAAARIRRALGAGEKITVFGDYDVDGVCSTALMVRVL
                     RELGGNVAAFIPSRFDDGYGLSCDALAACIAEHKPSLIVTVDCGTNSVEAVDKARACG
                     VDVVITDHHEPDAQLAHAVAVVNPKLAANHPGRILAGAGVAFKLCHALIKDGRDNGCA
                     VSASVDLKKYLDFVAVATVTDMVPLLGENRVLVRAGFQALENSCWAGWNALKKLAGMA
                     GTVETWHAGFALGPRINAAGRIGRADAAIELLLTDLPARADELAQLLDSANRERQAIE
                     KDIVREAIEEIDAYFDETKNFGIVIAREGWHTGVIGIVASRLVARYGRPVAVIGMDGN
                     RGRGSCRSIDAFNILDGLNICSGMLKQFGGHAMAAGLEVEEQNLEAFKIRFNEAAIAQ
                     LQSADLRPVLEIDRAVTL"
BASE COUNT          600 a          846 c          878 g          715 t
ORIGIN      
        1 ccggttcgtt cactgttgat gaatgcaccc gcctggtcaa cgtactgcgc gccggcgcgc
       61 tgcctgcgcc ggttaccatt gttgaagaac gcaccgtcga cccgtcgctt ggtaaagact
      121 ccatcgacag cggtatgcgg gcctgtatct acggcgcaat cgctgtggtc gtgttcatgg
      181 ccggttatta tctcctcgcc ggtgtgatcg ccgacctttc gctgttgatg ctgatgctga
      241 tgctgccctt cggaatgtgg ttttcctccg gcatactcag cctgttctcc gccggcgccg
      301 gtgcgtcggc cggactgccg gtgctgacgc tgcccggtat tgccggtatt gtgcttaccc
      361 tcgggatggc ggttgatgcg aacgttctga tttatgaacg tattcgcgaa gaactggcgc
      421 tgggtaagag tgtcgtcagt tccatttccg ccggttatga caaagctttc gccaccattt
      481 ttgactccaa tctcaccacg ctggtggcgg ctgtcatcat gttctggcag ggatcgggat
      541 ctatccgtgg attcgcggtt actctgacag ccggtattct cgtgagtatg ctcacagcgc
      601 tggtctttac ccgtctgctg cttgatacac tggcggcaaa gaccaaactg aagacgatta
      661 aaatgttttc attcttcccg aaaacaaaaa tcgattttat gggcaagcgg tatatcgcca
      721 tcggtctttc ctgggcgatc attatcatca gctggataat cctgttccat aagggcgaaa
      781 agaatttcgg cgtcgacttt accggcggca cctcgatcac gtttgccttt gatcagaaac
      841 agccggcgga agccgttcgc gcggttctcg aaaaggccgg catcaaagat gcagccattc
      901 agtatcacgg cgaactcggc gtggcggaag gtgccaaggc gaatgagacg cttgaagtca
      961 aagtcagtta cgaaaatggt aataccgcca ttgatgcggt aaaaaccgct tttgcggcgc
     1021 agggttacaa ggatattgcc aacgactctg tcggcccgca gatcggccag gaactgaagc
     1081 gcaagggcgc gatgtcgatg atactggctc tggtcggtat taccatttac atcgccttcc
     1141 ggtttgagtt cggttttgca atcggtgcaa ttgtggccac cgtgcacgac gtgctcatca
     1201 ccatcggtct ctactgcctg ttcggccgtg aactcaatct gacgattgtt gccgcattgc
     1261 tgacgattat cgggttctcg gtaaacgaca cgatcgtggt ttttgaccgt atccgcgaaa
     1321 acatcaagct gctgcacgga acaaaagcgt cgtatgctga aatttccaat ctcagcatca
     1381 accagacgct gtcgcgtacc gtcctgactt cattgacggt ggtactcacc gtgatttccc
     1441 tgctgatctt cggcggcggc gcgatctttg acttcgcact gacactcctg atcggtttca
     1501 cggtcggcac atactcgtcc atttatgttg ctacgccggt ggcgctgctc tggcacaaag
     1561 agaaaaaagg ctaagaacgc cgattccgga cggatgggac agcgccccga agtttcgggg
     1621 cgctgttttt ttgaaaatgg ctaaacagtg gaaatttaaa ccgtgcgacg aaacgctggc
     1681 tgagcatatt ttccggaaac tgggaaatat gccgaggccg cttacggcac tgctcgttca
     1741 acgcggctgc aaaaccgcgg cggaagccga gctgtttatg aatccggcgc tctcgactct
     1801 gcgcgatccg tttgagctgc ccgatatgga caaagccgcc gcgcgtatcc gccgcgctct
     1861 cggtgccggc gaaaagatca ctgtattcgg cgattacgac gtggacggcg tctgcagcac
     1921 cgcgctgatg gtgcgggttc ttcgcgaact cggcggaaat gtcgccgcct tcattcccag
     1981 ccggtttgac gacggctacg gcctttcgtg cgatgcgctg gccgcctgta ttgccgaaca
     2041 taaaccttcg ctgattgtca ccgttgactg cggcacaaac tccgttgagg ccgtcgataa
     2101 ggcgcgtgcc tgcggcgtgg atgtggtgat tacggatcat cacgaacccg atgcgcagct
     2161 tgcgcacgct gttgccgtcg tcaacccgaa gctggcggcg aatcatccgg ggcgtattct
     2221 cgccggtgcg ggcgttgctt ttaaactttg ccacgcactc attaaagacg gacgcgataa
     2281 cggctgcgcg gtttcggctt ctgttgacct caaaaagtat ctcgattttg tcgccgtcgc
     2341 gacggtcacc gatatggtcc cgctgctcgg cgaaaaccgt gtgctggttc gcgccgggtt
     2401 tcaggcgctg gaaaactcct gctgggcggg ctggaatgcg ctcaaaaagc tggccggtat
     2461 ggccggtacg gtcgaaacgt ggcacgccgg ttttgccctt ggcccgcgca tcaatgccgc
     2521 cgggcggata gggcgcgccg acgccgcgat tgaacttctt ctcaccgatc tgccggcgcg
     2581 cgccgatgaa ctggcgcagc tgctcgacag cgccaaccgc gagcgccagg cgattgaaaa
     2641 agatattgtc cgcgaagcga ttgaggagat tgatgcttat ttcgacgaga cgaaaaattt
     2701 cggtatcgtg attgcccgcg aaggatggca taccggcgtg atcggaattg tggcgtcgcg
     2761 cctcgtggcg cgctacggcc gtcccgttgc ggtaatcggt atggacggta atcgcgggcg
     2821 cggctcctgc cgcagtatcg acgccttcaa tattctcgac gggcttaaca tctgttccgg
     2881 catgttgaaa cagttcggcg ggcacgccat ggcggccgga cttgaagtcg aagaacagaa
     2941 tcttgaagcg ttcaaaatcc gttttaacga agccgcgatc gcgcagcttc agtccgccga
     3001 tctccggccc gtactggaaa tcgaccgcgc cgttacgct
//
LOCUS       DAOPZO010000174         3032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_44485_length_3032_cov_5.387974, whole genome shotgun sequence.
ACCESSION   DAOPZO010000174 DAOPZO010000000
VERSION     DAOPZO010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44485_length_3032_cov_5.387974"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..2045)
                     /locus_tag="PKI68_08735"
     CDS             complement(66..2045)
                     /locus_tag="PKI68_08735"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNX53815.1"
                     /translation="MDFFARQEKTRRNTAVLLVYFFAAVVGTAALVYVVFQLALFLLL
                     GQASSNHPSSVQFVWWQPSWFLLTFSATMMVVGISSMAKIIDLRRGGGSGVAELMGGR
                     QIQPDTSDFYERRLLNIVEEMAIAAGMTVPPVYVLIGEKGINAFAAGYTVRDAVVAVT
                     NGAMTGLTRDELQGVVAHEFSHIFNGDMRLNIHLMGNLYGLLVLTLIGRGILRAKVRG
                     KGAVYVYMIGGALLAVGSIGLLFGKLIKSAISRQREFLADASAVQFTRNPAGLAGALK
                     KIGGVAYGSQLSTAQAEAASHMFFSNGLRSSLFATHPPLAERIRWLEPSFNGKFERIT
                     QEKLCQALPVNEVAPQDASSSDIYTRSINVAAAGAALQPTPRSPSPAGPSNPDKLMAT
                     IGAPLQGHVQAASQLIESIPAELKAQARDPYAARAVICLLLLDNDESVRTNQLKILRG
                     QADPAVFAEFSKILPLRDSIPFEAKLPLLELTIPALKQLSRSQYLSFLADLLALIEAD
                     RRVSVFEYALERVLMNRIDPLFGGNKNKMRTAGHYAFCGVENEISWVLSAFARLNSDP
                     EAAFKKAAASVPDTRAKFALQPEAECGWKELDAALDKLSGASFYIKKWVLAAALVCLM
                     HDREITIEEVELFRAIADSLDCPVPPWLAATSLNK"
     gene            complement(2060..2662)
                     /locus_tag="PKI68_08740"
     CDS             complement(2060..2662)
                     /locus_tag="PKI68_08740"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNX53816.1"
                     /translation="MSGAAAGLLVLTGVCILILLAVVGAYNKLVTLRNRFKNAFAQID
                     VQLKRRYDLIPNLVETAKGYMGHERATLEAVIQARNQAVSAGKAAAQNPADGGAMAGL
                     VGAEGVLSGALGRLFALSEAYPDLKANQNMMQLTEELTSTENRISFARQAYNDAVTIY
                     NTAREVFPTVIIAGMFNFASAVLFEVADSTQKEAPKVSFS"
BASE COUNT          744 a          836 c          807 g          645 t
ORIGIN      
        1 tgcgctgctc gcctcgggac gtctcgatca aaaatcgccg ggccgcctgc cgtggagcct
       61 ctagttcatt tgttaagcga ggtggctgcg agccagggcg gcacgggaca gtccagactg
      121 tctgctatgg cgcggaataa ttcgacttct tcgattgtga tttcccggtc atgcatcagg
      181 cagaccagag ccgcagccag cacccatttt tttatgtaga aagaggcccc ggagagctta
      241 tcgagtgcgg catccagctc tttccaaccg cactccgcct caggctgaag cgcaaacttc
      301 gcccgggtat cgggaacgct ggcggcagct ttcttgaagg cggcttccgg atccgaattt
      361 aaacgggcaa aggcggagag aacccaggag atctcgtttt ccactccgca gaacgcgtaa
      421 tgaccggctg tacgcatctt gtttttattt ccgccaaaca gcggatcgat gcggttcatt
      481 agaacgcgtt ccagcgcata ctcaaaaacg cttacgcgac gatccgcttc gatcagcgca
      541 agtaaatcgg caaggaatga cagatactgg cttcgggaaa gctgcttgag cgccggaatt
      601 gtcagctcaa gcagaggcag tttggcttcg aaaggtatgc tgtcgcgcag cggaaggatt
      661 ttgctgaatt cggcgaagac cgccgggtct gcctgtccgc gcaatatttt gagctgattc
      721 gtacgcaccg attcgtcgtt atccagcaga aggagacaga tgaccgcccg ggccgcgtac
      781 ggatcgcgag cctgcgcctt gagttcggca ggaatggact cgatcaactg gctggcggcc
      841 tgcacatgcc cctgcaacgg cgcaccgatt gtcgccatca gcttgtcggg gttacttggt
      901 ccggctggcg acggggagcg cggtgttggc tgcagagcgg ccccggcggc cgccacgtta
      961 atggaacggg tgtagatatc cgaagaagat gcgtcctggg gcgcgacttc attgacgggc
     1021 agcgcctgac agagtttttc ctgtgtaatg cgttcaaatt ttccgttaaa ggagggctcc
     1081 agccagcgga tacgttcagc cagcggcggg tgcgttgcaa acagagaact ccggagtcca
     1141 ttgctgaaaa acatatgact ggccgcttcg gcctgtgctg tcgaaagctg cgaaccgtaa
     1201 gccacgccac cgattttttt cagtgcgccg gccagaccgg cggggttgcg ggtgaactga
     1261 accgccgagg cgtctgcaag aaactcccgc tgacgggaaa tcgcgctttt gatcagcttt
     1321 ccgaaaagga gtccgatgga accgaccgcc agtaaagccc cgccgatcat atatacataa
     1381 acagcgccct tgccgcgcac ctttgcccga agaatcccgc gaccgatcag cgtgaggacc
     1441 aacagtccgt acaggttgcc catcaggtgg atattcaggc gcatgtcacc gttaaatatg
     1501 tggctgaatt cgtgagcgac aacgccctga agttcatccc gcgtcaatcc ggtcattgca
     1561 ccattcgtaa ccgcaaccac tgcatcgcgc actgtgtagc ctgcggcaaa ggcattaatt
     1621 cccttttctc cgatcagcac atagacagga ggaactgtca tcccggccgc aatggccatt
     1681 tcctcgacga tattcagcag gcgccgctca tagaagtcgg aggtatccgg ctgaatctgc
     1741 cgccccccca ttaattcggc gacgcccgat ccgccgccac ggcgcaagtc gataatcttg
     1801 gccatcgagc tgattccgac gaccatcatc gtcgcactaa atgtgaggag gaaccaggat
     1861 ggctgccacc atacaaactg tacagaggac ggatgattcg acgaggcctg acccagtagc
     1921 agaaacaggg ccaactggaa cacaacataa accaaggctg ccgtgccgac tacggcggca
     1981 aaaaaataaa ccagcaaaac agccgtattc cggcgtgttt tttcctgacg ggcaaagaag
     2041 tccattcgga taccggatgt tatgaaaagc tgactttggg agcttctttc tgcgtgctgt
     2101 ctgccacttc aaacaacacc gccgaagcaa aattgaacat accggcaatt ataacggtcg
     2161 ggaaaacttc acgggccgta ttgtaaatgg ttacggcgtc gttgtaggcc tgccgcgcaa
     2221 aggaaatacg gttttcggta gaggttaatt cctcagtcaa ctgcatcatg ttctggttgg
     2281 ctttaagatc aggataggcc tcggaaagag caaacaaccg gccaagcgct ccgctcagta
     2341 cgccttccgc gccaaccagt ccagccatcg ccccgccatc ggccggattc tgcgcggcgg
     2401 ctttcccggc agacacagcc tgattgcgag cctgaatcac cgcttccagc gttgcacgtt
     2461 cgtgacccat gtaacccttg gcggtttcaa ccagattggg aatcaggtca tagcgtcgtt
     2521 tgagctgaac atcgatctga gcaaacgcgt ttttgaaccg gttgcgcagt gtaaccagtt
     2581 tgttatatgc gccaaccacc gcgagtaata tcagaataca tacccccgtc aaaaccagca
     2641 aaccagctgc cgctccgctc attttcgact ccttttcagc ttccgtttaa ttccacggga
     2701 aaataccgca gtaagaatgc gtgacatgtt agcaatatga acatcaactt gccaattttt
     2761 atatggtcca acaaaaaatc agccgccggg taaactgatt tttattgaaa cggctttgat
     2821 ttgaacgttc cgacgattag aaactccgca aactcggtta tattgggaat ataaaatcgg
     2881 agcatttgcc tgacatgaag tgctttcaca tataaagttc ggctgatgtg tttgcttcag
     2941 aaaactaaat caaaacggag aaaccgatga aaaaaggtaa gaccaaaacc gcagccaaaa
     3001 aaccggccgg cggcagcgca tggaagaaaa cg
//
LOCUS       DAOPZO010000175         3006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_45055_length_3006_cov_11.300237, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000175 DAOPZO010000000
VERSION     DAOPZO010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45055_length_3006_cov_11.300237"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PKI68_08745"
     CDS             complement(<1..187)
                     /locus_tag="PKI68_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53817.1"
                     /translation="MNLKQIITIGLIGLASVTVSRADLSGWAVYGTPVLGWNFDGDGA
                     SGGSVDSGSTATDWIASN"
     gene            complement(204..2762)
                     /locus_tag="PKI68_08750"
     CDS             complement(204..2762)
                     /locus_tag="PKI68_08750"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HNX53818.1"
                     /translation="MGIAVLGAAAVQGQTNQTAELQLTQFELYPYYLTNSLPISNMTV
                     DMNPPFLFCAPQGVSKNGTLYYFRLSQDDTFTSGVITNTPHRWSFYSPYTTLAQGVWY
                     WQYAYQPTGSVGSALSWSATQNFTILPNAWTNPARPVAVLESALISNGLPRAVCRSSD
                     VGNLMPQDATLRASLSNTVYAVLAKTPPTVIASDYENAVFISTYMNPALVGYALTADA
                     RFKNYATNAYAILVTNHATRTFGEFEENSFRKAAGNYYELFSSLLTPAEVATIREWGI
                     QDLSHQAEARMNFLEREPVNEHDWQSDVGALVPMALTYYDPADARARDALGYLYDLWN
                     FRGPVAGRTDGSWRPDGYFNAHIRTLLPVPALLSQYTGADLLGVPWYRNVAKMLAYSS
                     QPTYPHGAYGDGGEGWQFVYLYDVIKTLNVMRPGNPWNHRVWRMGDDKPTDIDFIAND
                     LIAMKLVWYALPWLHNQPLPSKADPIMTITNKAEVFRDAGLAVLCTDPNDQTNMVRVL
                     FRACPMGVYGHAHAGQNGFNITYGNQPVFYKTGYYSSSGDAFSLQDYKHTRGHNTILP
                     YGNVCQGLDTSGYGWMARFVNGNKIGYALGDASSSYSGSYRYHEGNLRANGIPITLEA
                     GFGKPGVTRFRRHLALLDTGTVVIYDELEATNAIPWTFQLNSIPPAVKINEHTVASAS
                     KNGIAVAELFCDSPVSTKLTDQFSGSYKTLYNNIVVLGKGYEPANHWHASITPTNALQ
                     KTRFLTLIHRVQNGQPVIQSVESVSADGRRVVSAGGWNVEAELDTGKPSFLKIWNNDN
                     TAALVSGQAATSLQLGSTTYTPQLPGSTILLEQGRAAQEAVDVLPDMMRYGNIY"
BASE COUNT          611 a          921 c          841 g          633 t
ORIGIN      
        1 tattgctggc aatccagtcc gtggctgtgg atccgctatc aacggaaccg ccggaagccc
       61 cgtcgccgtc aaaattccac cccagcaccg gcgtgccgta cacagcccag ccgctcagat
      121 ccgcccgcga taccgtcact gaagccagtc caatcaaccc tatcgtaatt atctgtttca
      181 gattcatcgt gctccttttc cgattaataa atgtttccgt agcgcatcat gtccggaagg
      241 acatcgaccg cttcctgcgc cgccctgccc tgttcgagca gaatggtgct tcccggcagt
      301 tgcggcgtgt aggtggtcga gccgagctgc aggctggtcg ccgcctggcc gctcaccaat
      361 gccgccgtgt tgtcgttgtt ccagatcttc aggaacgagg gtttgcccgt atccagctcc
      421 gcttcgacgt tccagccgcc ggccgaaacc acccgccggc catctgccga gacgctttcc
      481 accgactgga tcaccggctg gccgttctgc acgcggtgga tcagcgtcag gaagcgcgtc
      541 ttctgcagcg cattggtcgg cgtgatgctc gcgtgccagt ggtttgccgg ctcatatcct
      601 ttgcccagca ccacgatgtt gttgtagagc gttttgtagc tgcccgagaa ctgatcagtc
      661 agcttcgtcg aaaccgggct gtcgcagaac agctctgcaa cagcgatacc gtttttagag
      721 gccgaggcca ccgtgtgttc gttgattttg accgccggcg gaatcgagtt gagctggaag
      781 gtccacggga tcgcattggt ggcttccagt tcgtcgtaga tcaccacggt tccggtatcc
      841 agcagcgcca gatggcgccg gaagcgcgtg acgcccggct tgccgaaccc ggcttccagc
      901 gttatcggaa tcccgtttgc tctcaggttg ccctcatgat agcgataact gccgctgtag
      961 gatgatgaag catcgcccag ggcgtagccg attttgttgc cgttcacaaa ccgcgccatc
     1021 cagccgtagc cgctggtgtc gagcccctgg cagacgttgc cgtagggcag aatcgtgttg
     1081 tggccccggg tatgtttgta gtcctgcagc gagaacgcat cgcccgacga gctgtaataa
     1141 cccgtcttat agaacaccgg ctggttgccg taggtgatat tgaagccgtt ttggcctgca
     1201 tgggcatgtc cgtacacccc catcgggcag gcgcgaaaca gcacgcgcac catattcgtc
     1261 tgatcgttcg gatccgtgca gagtaccgcc agccccgcat cgcggaaaac ctccgctttg
     1321 ttggtaatgg tcatgatcgg gtcggctttg gacggcagcg gctggttgtg cagccagggc
     1381 aaggcgtacc acaccagttt catggcgatc agatcattcg cgataaaatc aatatcggtc
     1441 ggtttgtcat cgcccatccg ccagacccgg tggttccacg gattgcccgg acgcatcaca
     1501 ttgagcgtct tgatcacgtc gtacaaatag acaaactgcc agccttcgcc gccatcgccg
     1561 taggccccgt gcggataggt cggctggctg gaataggcga gcatcttggc gacgttgcga
     1621 taccacggca cacccagcag gtcggctccc gtgtactgcg acaaaagcgc cggcaccggc
     1681 agcagagtcc ggatgtgcgc attgaaatag ccgtcagggc gccagctgcc gtcggtccgc
     1741 ccggcgaccg ggccgcggaa attccataaa tcatagagat agccgagcgc atctcgggcg
     1801 cgggcatccg ccggatcgta ataggtgagc gccatcggca ccagcgcccc cacatcggac
     1861 tgccagtcat gctcgttcac cggctcgcgt tccagaaaat tcatgcgcgc ttccgcctgg
     1921 tgcgacagat cctgaatgcc ccattcgcgg atcgtggcca cctcggccgg cgtcagcagg
     1981 gaactgaaga gctcgtaata attgcctgcg gctttccgga acgagttttc ttcgaattcg
     2041 ccgaacgtcc gcgtcgcatg gttcgtcacc agaatcgcat aggcgttggt tgcgtagttt
     2101 ttaaagcgcg catccgccgt caacgcatag cccaccagcg ccggattcat gtaggtggag
     2161 ataaacaccg cattttcata gtcgctcgca atcaccgtgg gcggcgtctt ggccagcacc
     2221 gcatacaccg tgttgctcag actcgcccga agcgtcgcgt cctgcggcat cagattaccg
     2281 acatccgacg accggcaaac ggcgcgcggc aggccgtttg aaatcagggc cgactccagc
     2341 acggcgaccg gccgggccgg gttcgtccac gcattcggaa gaatggtgaa attctgcgtc
     2401 gcggaccagc tcagcgccga gccgacgctg ccggtcggct gataggcgta ctgccagtac
     2461 cagactccct gcgccagagt cgtatacggc gaataaaacg accagcggtg cggtgtgttg
     2521 gtgatcaccc cgctggtaaa cgtatcgtcc tgggagagcc ggaaatagta gagcgttccg
     2581 tttttagaaa ccccttgagg ggcacaaaac aaaaacggtg gattcatatc caccgtcata
     2641 ttgctgattg gcagcgaatt ggtcagatag tacggataca gctcaaactg cgtcagctgt
     2701 aattccgccg tctggttcgt ctggccttga accgccgcgg cacccagcac tgcgataccc
     2761 attaacagtc tctgtacgat ccggcgtaac cggaacatgc tccgggaaac gccgccattt
     2821 acatgcatca tcgtcataag cacccctcgc tgttcaccgt cccgcaacgt ctgacgcaaa
     2881 gcctaaccct gcgttatttc aaacgtcaca ggaacaaaaa gccgtaaatt gagtattcat
     2941 tttagtgaaa aaaagaccaa tctccgatga ctgattaggt ttaatcgtac acccttaccg
     3001 actcgt
//
LOCUS       DAOPZO010000176         2954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_46260_length_2954_cov_8.480511, whole genome shotgun sequence.
ACCESSION   DAOPZO010000176 DAOPZO010000000
VERSION     DAOPZO010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46260_length_2954_cov_8.480511"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1478)
                     /locus_tag="PKI68_08755"
     CDS             complement(72..1478)
                     /locus_tag="PKI68_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX53819.1"
                     /translation="MFSRKNKPVRMAFSLRLALIYTVALCVSLAALSAVTYQLVRRVV
                     QNRDHEVIEAQTSQFKSLFERGGVQAIASYFRQQINAPQEPSFVRIVDDFNQVHFITV
                     SHQLWTLLDKKTGGPPPLDRAHWDELFREETDGSWIVGTTPLAPGLYLQVGRNNTESR
                     LVLANFKKTALNIFLPAVVFSLLTGWLVTRSALTPLHALIETVRHILATGDLQRRVPS
                     GAKRGELNELTAMFNRMLDQNEKLIRGSRETLDNVAHDLRTPMTHLRNSAERALQLPE
                     PDHTALQNALADCVEESEQILRMLNVLMDLAEAGAGSLRLDRQDIALRELADGVIELY
                     ALVAEERGITLTNEVPAACTVNADRLRLRQCIANLVDNALKYSPDGSAVTLSGSCADG
                     TVSFSVQDQGAGIAHDDLPRIWDRLYRAEQSRTTPGLGLGLSLVRAIIEAHGGTVAVQ
                     SEPGRGSTFTLRLPAVQG"
     gene            complement(1459..2133)
                     /locus_tag="PKI68_08760"
     CDS             complement(1459..2133)
                     /locus_tag="PKI68_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX53820.1"
                     /translation="MRILVIEDDRKISSFLEKGFREAGCAVDTAADGEEGLALARQDF
                     YDAIIADLMLPKLDGLDMITRLRAEKVNTPVLILSAKRSVDDRIKGLQAGGDDYMVKP
                     FSFSELLVRVQTLLRRASRTPEPMTLRVEGLTLDIARRTVERNGQTIELQAREFALLE
                     YLMRNAGRVVTKTAILENVYDYSFDPQTNVVDVLVCRLRNRIDHDFEEKLIHTVRGAG
                     YVLKKK"
     gene            2348..2950
                     /locus_tag="PKI68_08765"
     CDS             2348..2950
                     /locus_tag="PKI68_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNX53821.1"
                     /translation="MKTIHLKPCSAGATLALLLTASTACAGNTNGNVEVKTEAAAQNA
                     PAEQQAPDYLDPVREMIRMNREMDRFFGQPFASFSVMPRMAAELEGQFAKPDMDLREQ
                     AEAYYVQLDLPGMDKSAISVEVKDNILTVKAERKQETAKKGGDRVLMQERSCGFMSRA
                     VMLSKPVDAEKVTAEYKDGVLKVTLPKVHADQAPKQVQIK"
BASE COUNT          561 a          817 c         1009 g          567 t
ORIGIN      
        1 tgcggcgcgg ccggacggat cgtcccggac gggttgcaga tgagagaaac gctcttttgt
       61 ccgtcgcgcc attaaccctg aactgcgggc aggcggaggg tgaaggtgga gccccggccg
      121 ggttcgcttt gaaccgcgac ggtgccgccg tgcgcttcga tgatggcgcg gacgaggctg
      181 aggccgagcc ccagaccggg cgtggtgcgg ctctgctccg cgcggtacag ccggtcccag
      241 atgcgcggca gatcgtcgtg agcaatgccc gcgccctgat cctgaacgga aaacgaaacc
      301 gttccgtcgg cgcagctgcc ggacagcgtg acggcacttc cgtcggggct gtatttgagc
      361 gcgttgtcga cgaggtttgc aatgcattga cgcaggcgca ggcggtcggc gttgacggta
      421 cacgcggccg gcacctcgtt cgtcagtgtg atgccgcgtt cttcggcgac cagggcgtac
      481 agctcgatca ctccgtcggc caattcgcgc agggcgatgt cctggcggtc caggcgcagg
      541 ctgccggcgc cggcttcggc cagatccatc agtacattca gcatgcgcag aatctgttcg
      601 gactcctcga cgcagtcggc gagcgcgttc tgcagcgcgg tgtgatcggg ttcggggagc
      661 tggagcgcac gctccgcgga gttgcgcagg tgggtcatcg gggtgcgcag atcgtgcgcg
      721 acgttatcga gggtttcgcg cgagccgcgg atcagttttt cgttctggtc gagcatgcgg
      781 ttgaacatcg cggtgagttc gttcagttct ccgcgctttg cgccggaagg gacgcggcgc
      841 tgcagatcgc cggtggcgag aatatgccgg acggtttcga tcagcgcgtg cagcggtgtc
      901 agcgcgctgc gggtcaccag ccagccggtg agtaagctga aaacgacggc gggcaggaaa
      961 atgttgagcg cggttttttt gaagttggcg agcacgagcc ggctttcggt gttgttgcgg
     1021 ccgacctgca gatagagtcc gggtgcgagc ggtgtggtgc cgacaatcca gctgccgtcg
     1081 gtttcttcgc ggaacagttc atcccagtgc gcccggtcca gcggcggcgg gccgccggtt
     1141 tttttgtcga gcagggtcca gagctgatgc gatacggtga tgaagtggac ctggttgaag
     1201 tcatcgacga tgcggacaaa ggagggttcc tgcggcgcgt tgatttgctg tctgaaatag
     1261 gaggcgatgg cctgaacccc gccgcgctcg aagagcgatt taaactggga ggtctgggct
     1321 tcgatcactt cgtggtcgcg gttctgcacg acgcggcgga cgagctggta ggtgacggcg
     1381 gagagcgcgg cgagcgaaac gcagagcgcg accgtataga tcagtgccag ccgcaggctg
     1441 aaggccatcc ggaccggttt attttttctt gagaacatag ccggctccgc ggacggtgtg
     1501 gatcagcttc tcttcaaagt catggtcgat gcggttgcgc aggcggcaga cgaggacgtc
     1561 gacgacgttg gtttgcgggt cgaagctgta atcgtagaca ttttcaagaa tcgctgtttt
     1621 ggtgaccacg cggccggcat tgcgcatcag gtattcaagc agcgcgaatt cccgcgcctg
     1681 cagttcgatg gtctgcccgt tgcgttcgac ggtgcggcgg gcgatgtcca gcgtgaggcc
     1741 ttccacccgc agggtcatcg gttccggtgt gcggctggcg cggcgcagca gggtttgaac
     1801 gcgcaccagc agctcggaga aggaaaaggg tttgaccatg taatcgtcgc cgccggcctg
     1861 caagcctttg atccggtcgt cgaccgagcg tttggcgctc aggatcagca ccggcgtatt
     1921 gaccttttcg gcgcgcaggc gggtgatcat gtcgagtccg tcgagcttcg gcagcatcag
     1981 gtcggcgatg atggcatcgt aaaaatcctg ccgggccagt gcgaggcctt cttcgccgtc
     2041 ggcggcggtg tccacggcgc agcccgcttc ccggaagcct ttttccagga acgatgaaat
     2101 tttccggtcg tcttcgatca ctaaaatccg cacggcattc tcctgtctgc aaacgatcca
     2161 gaactaatac cgggcgccgg ttttgtcaat ggccggaacc attaccgctt tgtaatgcgc
     2221 cggcaagaat cccgaaaggt ggctgcggtt tattggcgcc agaaaaaccg ggggaggtga
     2281 tgccgatggc gtgacgttcc tttcaaaccc aaaccgagag cgaagtaaga ttctggaggt
     2341 agagaaaatg aaaacgattc atttaaaacc ctgttcggcc ggcgccacgc tggcgctgct
     2401 gctgacggcg agtacagcct gtgccgggaa cacgaacggg aatgtagagg tcaaaacaga
     2461 agccgcggcg cagaacgcgc cggcggaaca gcaggcgccg gactacctgg acccggtgcg
     2521 tgaaatgatc cggatgaacc gcgaaatgga ccgcttcttc ggccagccgt tcgcctcatt
     2581 cagtgtcatg ccgcgaatgg cggcggagct ggaaggccag ttcgccaagc cggacatgga
     2641 tctgcgcgag caggcggaag cctattacgt gcagctggat ctgccgggga tggataaatc
     2701 cgccatctcc gtcgaagtga aggacaacat cctgaccgtc aaagccgaac gcaagcagga
     2761 gaccgccaaa aaaggcggcg accgggtgct gatgcaggaa cgctcctgcg gctttatgag
     2821 ccgcgcggtg atgctttcca agccggttga cgccgagaaa gtgaccgccg aatacaaaga
     2881 cggcgtgctg aaggtcacac tgccgaaagt tcatgccgat caggctccga agcaggtgca
     2941 gatcaaataa ccgc
//
LOCUS       DAOPZO010000177         2948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_46401_length_2948_cov_12.349810, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000177 DAOPZO010000000
VERSION     DAOPZO010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46401_length_2948_cov_12.349810"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1832
                     /gene="fusA"
                     /locus_tag="PKI68_08770"
     CDS             <1..1832
                     /gene="fusA"
                     /locus_tag="PKI68_08770"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNX53822.1"
                     /translation="EVERSLRVLDGAIALFCAVGGVQPQSETVWHQSEKYSVPKIAFV
                     NKMDRVGADFFGVVKGIQEELGGNAVPVVIPIGSAEEFAGIIDLVKMVAIYYDEASKG
                     VDFEEKEIPAELLEQAKEWRNNLVEKCAEQDEALMEKYFAGEDLTADEIRTILRKATI
                     ARKVVPVYCGSAFKNKGVQRVLDGVVYLLPAPNEIPPIICEKGGEGVTRQPKDDDALS
                     ALAFKIMSDKHMGKLTYVRVYSGMLTSGSNIYNSTRDRMQRVGRVLRMHANKQEAIDA
                     AYAGDIVAVVGLTETKTGDTLCDKDKPIYLEAMEFPAPVISISVAPESKMDNEKLGEA
                     LHRLADEDPTFVVSFDQETSETIISGMGELHLEIIVDRLKREFGVQAKVGRPEVAYRE
                     TATVATDGSYKHAKQSGGRGQYGHVVLELEPRGPGEGFEFNNKVVGGRIPQEYIPSVE
                     KGIIQALQSGPYAGYPVVDMRVNLVDGSYHDVDSSDFAFQIAARQGFKQLFLKGSPQL
                     LEPIMSIEITTPEEYMGPASGSICQRRGRIESMDSRGGMKLIRGFAPLSEMFGFSNTI
                     RSFSQGRASFTMHFEHYEAVPFSIAEEVVKKRRAEGKVRGGEE"
     gene            complement(1903..2382)
                     /locus_tag="PKI68_08775"
     CDS             complement(1903..2382)
                     /locus_tag="PKI68_08775"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNX53823.1"
                     /translation="MKTAGILGALVLAAAVLCGCSQKAAAADQAGIWKPDYDAALKQA
                     AAENKYVLVSISGLQWCGWCKALENEVFSKPEFIGYARTNLVCVLLDFSRSGRATNTE
                     FAKQHEELLQRYQVEGFPTVLILSPQGKVVKRDGYQPGGAAKYVEFIKGVIAADQKK"
     gene            2473..>2948
                     /locus_tag="PKI68_08780"
     CDS             2473..>2948
                     /locus_tag="PKI68_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNX53824.1"
                     /translation="MDFIAFDLETTGIQPKTDAVIEIGAVRFRNAEAVGTFSTLINPG
                     RPIPPEASAVNGITDDMVAGRAGIETVLTELADFCGELPLVAHNAPFDFKFLLAAVEK
                     HRARAPKGVVLDSCALARIVFPGMFNYKLSTLVRHFGFPNGTFHRAEEDSVYCGLL"
BASE COUNT          642 a          904 c          803 g          599 t
ORIGIN      
        1 tggaagtgga gcgctccctg cgcgtactcg acggcgcgat cgcgctgttc tgcgccgtcg
       61 gcggcgtcca gccccagtcg gaaaccgtct ggcaccagtc ggaaaagtac agcgttccga
      121 aaatcgcgtt cgtgaataaa atggaccgcg tcggcgccga tttcttcggc gtggtgaaag
      181 gcattcagga agagctgggc ggcaacgccg tgccggtggt tattccgatc ggctccgccg
      241 aagagttcgc cgggatcatc gacctcgtca agatggtcgc catctattac gacgaagcca
      301 gcaaaggcgt tgatttcgaa gaaaaagaaa ttcccgcaga actgctcgag caggccaaag
      361 aatggcgcaa caatctggtt gaaaaatgcg ccgagcagga cgaagccctg atggaaaaat
      421 attttgccgg cgaagacctg acggcggatg aaatccggac catcctgcgc aaagccacca
      481 tcgcccgcaa agtggttccg gtttactgcg gttccgcctt caagaacaaa ggcgtccagc
      541 gcgttctcga cggcgtggtc tacctgctgc cggccccgaa cgaaattccg ccgatcatct
      601 gcgaaaaagg cggcgaaggg gttacccgtc agccgaaaga cgacgatgca ctttccgctc
      661 tggccttcaa gatcatgtcc gataagcaca tgggcaagct gacctacgtc cgtgtttatt
      721 ccggcatgct gacctccggc tccaacattt acaacagcac ccgcgaccgg atgcagcgcg
      781 tcggccgcgt tctgcggatg cacgccaata agcaggaggc gattgacgcc gcttacgccg
      841 gcgacatcgt ggccgtggtc ggtttgacag aaacgaaaac cggcgacacc ctgtgcgaca
      901 aagacaaacc gatctatctc gaagcgatgg aattcccggc tccggtgatc tccatcagcg
      961 ttgctcccga atccaagatg gataacgaaa aactcggcga agccctgcac cgtctggccg
     1021 atgaggatcc gacgttcgtg gtctccttcg accaggaaac cagcgaaacc atcatctccg
     1081 gcatgggtga gctgcacctt gaaattatcg ttgaccgtct gaaacgcgaa ttcggtgtgc
     1141 aggcgaaagt cggccgccct gaagtggctt accgcgaaac cgccaccgtg gccaccgacg
     1201 gttcctacaa gcacgccaaa cagtccggcg gacgcggcca gtacggccac gttgtgctcg
     1261 aactcgagcc gcgcggtccc ggcgaaggat tcgaattcaa caacaaggtg gtcggcggac
     1321 gcattccgca ggaatacatc ccgtcggtcg aaaagggcat tatccaggcg ctgcagagcg
     1381 gtccgtatgc cggttacccg gtggtcgata tgcgcgtcaa cctcgtcgac ggttcgtacc
     1441 acgatgtgga ctcgagcgac ttcgccttcc agattgccgc acgccagggc ttcaagcagc
     1501 tcttccttaa aggcagcccg cagctgctcg agccgatcat gtcgatcgaa atcacgacgc
     1561 ccgaagaata catgggcccg gcctccggca gcatctgcca gcggcgcgga cgtattgagt
     1621 cgatggacag ccgcggcggc atgaagctga tccgcgggtt tgctccgctg agcgaaatgt
     1681 tcggcttctc gaacaccatc cgctcgttct cccagggacg cgcttcgttc accatgcact
     1741 tcgaacacta cgaagccgtg ccgttctcga tcgccgaaga agtggtcaag aaacgccgcg
     1801 ccgaaggtaa ggttcgcggc ggcgaagaat aaaattcttc atcctgcgca aacaacaagc
     1861 cgctccggaa acggggcggc ttttttattc gctgtccggt gtttattttt tctgatccgc
     1921 agcgattaca cctttgatga actcgacata tttcgccgcg ccgccgggct gatagccgtc
     1981 gcgcttcacc accttgccct gcgggctgag aatcagcacg gtgggaaagc cttcaacctg
     2041 atagcgctgc agcagctctt catgctgctt ggcaaattcc gtattggtcg cgcgtccgga
     2101 gcggctgaag tcgagcaata cgcaaaccag attcgtccgg gcatagccga taaactccgg
     2161 cttggaaaac acttcgtttt ccagcgcctt gcaccagccg caccattgca ggccgctgat
     2221 acttaccagt acatatttat tttctgccgc cgcctgtttc agagcggcat cataatccgg
     2281 cttccaaatt cccgcctgat ccgccgccgc cgctttctgg ctgcatccgc ataaaacggc
     2341 cgccgccaga accaaagctc cgagaatacc tgctgttttc atgatgcctc ctttttctgc
     2401 ggccagcata cccgtccaat aaaaccgtgc caagccttga gcgcactctt ctgttacagt
     2461 ataagagacc gtatggactt tattgcgttc gatcttgaaa ccaccggcat tcagccgaaa
     2521 acggatgccg ttattgaaat cggcgcggtg cgtttccgta atgccgaagc ggtcggaact
     2581 ttcagcacgc tcatcaaccc cggtcgcccg attcctccgg aagcctctgc cgttaacggc
     2641 attaccgacg atatggttgc cggccgggcc ggaatcgaaa ccgttctcac ggaacttgcc
     2701 gatttttgcg gcgaacttcc gctggtcgct cacaacgcgc cgttcgattt taaatttctg
     2761 ctggcggccg ttgaaaagca ccgcgcccgc gcgccgaaag gcgtcgtgct cgacagctgt
     2821 gcgctggccc gcatcgtctt ccccggcatg ttcaactata aactcagcac cctcgtccgc
     2881 cattttggat ttccgaacgg cacgtttcac cgcgccgaag aagacagcgt ctattgcgga
     2941 ctgctttt
//
LOCUS       DAOPZO010000178         2920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_47087_length_2920_cov_10.367888, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000178 DAOPZO010000000
VERSION     DAOPZO010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47087_length_2920_cov_10.367888"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="PKI68_08785"
     CDS             complement(<1..1272)
                     /locus_tag="PKI68_08785"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNX53825.1"
                     /translation="MAGIYKAYDIRGIYGKDLTDDIAFKIGRAVATFLKPKTFVIGHD
                     MRPHSIPLFNAMAKGLTMQGVNVINIGLVSTPMSYHANGKLGADGSAIITASHNPGEW
                     NGMKICREKAIPISGVTGIADIERIVNEESFDAPAAKPGSVTSYDIKSEYFAHIRTFA
                     KIKKPLNVVIDYANGMGISEGGAFTGEMFNITPMFDVLDGTFPNHEANPLDLHTLHDI
                     SAKLKAGKYDFGIAFDGDADRAGFLDENGDVVTMDMITALVAQSMLEEFPGAAIFYDL
                     RSSWAVKEVIEEAGGRPMMSRVGHAFIKAQMREADAVFAGELSGHYYFRDNYYTESSS
                     LAAICVANLISKTGKTLSQLIKPLKRYVGSGEINSKVADVQTVFKKLDAKYGDARKFQ
                     LDGNSYEYADWWFNVRSSNTEPLVRLNLEAKT"
     assembly_gap    1288..1387
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1388..1668)
                     /locus_tag="PKI68_08790"
     CDS             complement(<1388..1668)
                     /locus_tag="PKI68_08790"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNX53826.1"
                     /translation="MKSKIYRAILGLFLLAVSPSGFAENGPKLVCEVPVYQFGRTNQA
                     AVVTHVFMIRNEGDTTFVAGMPRTTCGCTKARISKRMIGPGETAELTAV"
     gene            complement(1701..2081)
                     /locus_tag="PKI68_08795"
     CDS             complement(1701..2081)
                     /locus_tag="PKI68_08795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNX53827.1"
                     /translation="MNWENINQLCDVIRETSFSIHSYHRSGHLEKIYENALANRLRKL
                     GLNVKQQHPLTVYDEDGTVLGEYFADLFIEGCLIVELKACRDIAPEHAAQIIGYLTSS
                     RIETGLLINFGSAKLQVKKYLQST"
     gene            complement(2114..>2920)
                     /gene="ruvB"
                     /locus_tag="PKI68_08800"
     CDS             complement(2114..>2920)
                     /gene="ruvB"
                     /locus_tag="PKI68_08800"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNX53828.1"
                     /translation="YIIAEAMGANIKCTSGPVIDKPGDLAGLLTSLERGDFLFIDEIH
                     RMQKSVEEYLYSAMEDFVIDIVIDQGPNARSVRLNIQPFTLIGATTRSGLLSAPMRSR
                     FGLVNRLDYYDAATLQKIIERSARILNVDIEPDGALEIARRSRGTPRIANNLLRRARD
                     YAQVKAGNVITADVAAKALALLEIDESGLDEMDKKILSTIIDKFAGGPVGISSLAVSV
                     GEEAGTIEEVYEPYLIQEGFIKRTPQGRVVLPRGYDLFGLKPGNSQQELL"
BASE COUNT          563 a          743 c          840 g          674 t
ORIGIN      
        1 ggttttggct tcgaggttga ggcggacgag cggctcggtg ttggaggagc ggacgttgaa
       61 ccaccagtcg gcgtattcat aggaattgcc gtcgagctgg aacttgcggg cgtcgccgta
      121 tttggcgtcg agctttttga aaaccgtctg cacatcggca accttggagt tgatttcgcc
      181 ggagccgaca tagcgtttga gcggcttgat gagctgcgaa agcgttttgc cggttttgct
      241 gatgaggttg gcgacgcaga tggcggccag cgaggagctt tcggtgtaat agttgtcgcg
      301 gaagtagtag tggccggaga gttcgccggc gaagacggcg tcggcttcgc gcatctgcgc
      361 tttgatgaag gcgtggccga cgcggctcat catcgggcgg ccgcccgctt cttcgatgac
      421 ttctttgacg gcccagctgc tgcgcaggtc gtagaagatc gcggcgccgg gaaattcttc
      481 gagcatggac tgcgcgacca gcgcggtgat catgtccatg gtaacgacgt cgccgttttc
      541 gtcgaggaag ccggcgcggt cggcgtcgcc gtcgaaggcg ataccgaagt catacttgcc
      601 ggctttgagc ttggcggaaa tgtcgtgaag cgtgtgcagg tcgagcgggt tggcttcgtg
      661 gttcgggaat gttccgtcga gcacgtcgaa catcggagtg atattgaaca tttcgccggt
      721 gaaggcgccg ccttcggaaa tgcccatgcc gttggcgtag tcgatgacga cgttgagcgg
      781 cttcttgatc ttcgcgaagg tgcggatgtg ggcgaaatat tcggatttga tgtcatagct
      841 cgtcacgctg ccgggcttgg ccgccggagc gtcgaaggat tcttcgttga cgatgcgttc
      901 gatgtcggcg atgccggtga cgccgctgat cggaatggct ttttcgcggc agattttcat
      961 gccgttccat tcgccgggat tgtgcgaagc ggtgatgatg gcggagccgt ccgcaccgag
     1021 cttgccgttg gcgtggtagg acatcggagt agagaccagc ccgatgttga tgacgttcac
     1081 gccctgcatc gtcaggcctt tggccattgc gttgaagagc ggaatggagt gcgggcgcat
     1141 gtcgtgcccg atgacaaagg ttttgggttt gaggaaggtg gcgaccgcgc gcccgatttt
     1201 gaaggcgatg tcgtccgtca ggtcttttcc gtagattccg cgaatgtcgt atgctttgta
     1261 aatgccggcc atgattctct cctgttannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnaat accgccgtca gctccgccgt ctcgcccggt ccgatcatcc gcttgctgat
     1441 ccgggctttt gtacagccgc aggtggtacg cggcattccc gccacaaagg tggtgtcgcc
     1501 ctcgttgcgg atcataaata catgggtgac taccgccgcc tggttcgtgc gcccaaactg
     1561 ataaaccgga acctcgcaca ccagcttcgg gccgttttcc gcaaatccgg acggagaaac
     1621 cgccagcaga aaaagcccta atatcgcccg atagattttt gatttcattt tattagccac
     1681 aaaaaacaca aaaaccaacc ctaagtactc tgaaggtatt ttttaacctg aagctttgcc
     1741 gagccgaaat ttatcaaaag gccggtttca atccgggagg aagtcagata gccgattatt
     1801 tgagcagcat gctccggcgc aatatcccgg caggctttca attccacaat cagacatcct
     1861 tcaataaaaa gatccgcaaa atattcgccc aatacggttc catcttcatc ataaaccgtt
     1921 aatggatgct gctgttttac atttaatccc agcttacgca gacggtttgc caaagcattt
     1981 tcatatattt tttctaaatg cccgctccgg tggtaagaat gaattgagaa actcgtctcc
     2041 cggatcacat cacatagttg attgatattt tcccaattca ttccacatct catttttgtg
     2101 ttttttgtgg caattaaaga agttcttgct gagagtttcc gggtttgaga ccgaacagat
     2161 cgtaaccgcg cggcagaaca acgcgtccct gcggcgtgcg cttgatgaag ccttcctgaa
     2221 tcagatacgg ctcatacacc tcttcaatcg tccccgcctc ttcgccgacc gaaaccgcca
     2281 gcgaactgat gccgaccggg ccgccggcga atttatcgat gatggtggaa agtatctttt
     2341 tatccatctc gtcgagaccg ctctcgtcga tttccagcag cgccagcgct ttcgcggcga
     2401 cgtcggcggt gatgacgtta ccggctttga cctgtgcata gtcgcgggcg cggcgcagca
     2461 ggttgttggc aatacgcggc gtgccgcgcg agcggcgggc gatttccagc gcgccgtccg
     2521 gttcgatatc cacattcaga atgcgcgcgg agcgctcgat gattttctgg agcgtcgccg
     2581 catcgtaata gtcgagccgg ttgaccagac cgaagcgcga gcgcatcggc gcggaaagca
     2641 gcccgctgcg ggtcgtcgcg ccgatcagcg tgaacggctg aatgttcagc cgcaccgaac
     2701 gggcgttcgg cccctggtcg atgacgatat cgatgacgaa atcttccatc gcggaataga
     2761 gatattcctc aaccgacttc tgcatgcggt ggatttcgtc gatgaacagg aagtcgccgc
     2821 gctccagact ggtcagcaga ccggcgaggt cgccgggctt gtcgatgacc gggccggagg
     2881 tgcatttgat atttgcgccc attgcttcgg cgatgatgta
//
LOCUS       DAOPZO010000179         2908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_47358_length_2908_cov_8.932352, whole genome shotgun sequence.
ACCESSION   DAOPZO010000179 DAOPZO010000000
VERSION     DAOPZO010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47358_length_2908_cov_8.932352"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PKI68_08805"
     CDS             complement(<1..519)
                     /locus_tag="PKI68_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53829.1"
                     /translation="MAAKKVSKKKTAAKAVKKAPAKKAVEKTVKKAPAKKAAAKTAAK
                     PAAVKPADKKVLPVHVHKPAAPAPALPAARKLTSKEIREFKDILLTLRERVSGEYTTL
                     SRDNLEANQRDPSLSDQGTDTFDREMELNMMGGEQEVLFEIDAALRRIEKGTFGICEL
                     TGQPINIERLQAL"
     gene            complement(635..1402)
                     /locus_tag="PKI68_08810"
     CDS             complement(635..1402)
                     /locus_tag="PKI68_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX53830.1"
                     /translation="MRITFLGTGTSHGVPMIGCRCAVCRSDNPKNKRRRCSLYVQAGG
                     KHLLFDTPPDLRDQVLTFGVERVDAVFITHPHADHIFGFDDVRRFSELQEAHLPVYGS
                     PETIASMRTKFDYVDKPSHSFGAVPRVVFTGMSAPVEAGGARVTPVPVKHGQDDVYGF
                     LVEADGKRLAYIPDCSGIPPESFSRLENLDVMILDGLRPGRHATHFSIEECVEHLQRF
                     GAKRSFLTHLTHHSDHDTLQAALPAGIEVPWDGLTIA"
     gene            complement(1399..2556)
                     /gene="nspC"
                     /locus_tag="PKI68_08815"
     CDS             complement(1399..2556)
                     /gene="nspC"
                     /locus_tag="PKI68_08815"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR01047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HNX53831.1"
                     /translation="MNFQTVPTPSFVVDEALLQRNLDTLALVKKRTGCKILLALKGFA
                     MWSTFPLLRKTLDGVCASSPWEARLGREEFDGEVHAFAAAYSEADVRELLILCDEIDF
                     NSFAQWKKFRPLVERAVPARLNVRGAPVPPKFGLRINPECSTGRTPMYDPCAPKSRLG
                     ITRDQFAGESLEGISGLHFHTLCEQNADALEITLKAIEEKFGEFLPQMEWVNFGGGHH
                     ITRADYDIDLLCKLINDFKAKYGVQVILEPGEAVALNTGVLITTVLDVVQNASEIAIL
                     DCSCTCHMPDVLEMPYRPAITGAGQAGEKAYTYRLAGMSCLAGDVIGDYSFDRPLKPG
                     DRLVFEDMAHYTMVKTTMFNGIKHPSIAIQRGDNVEIVREFHYEDYKNRLS"
     gene            complement(2662..2886)
                     /gene="infA"
                     /locus_tag="PKI68_08820"
     CDS             complement(2662..2886)
                     /gene="infA"
                     /locus_tag="PKI68_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNX53832.1"
                     /translation="MAKQDDSLISLDGTVTEVLPGTMYRVKFDNGHTVLAHVSGKIRK
                     NFIRISIGDRVTVEISTYDLTKGRITYRHA"
BASE COUNT          597 a          834 c          820 g          657 t
ORIGIN      
        1 cagagcctga agacgttcga tattgatcgg ctggccggtc agttcgcaga taccgaaagt
       61 acctttttcg atacggcgca gcgccgcgtc gatttcaaag agaacttcct gttcgccgcc
      121 catcatgttg agttccattt cgcggtcaaa cgtatcggta ccctggtcgg acagcgacgg
      181 atcgcgctgg ttggcttcga gattgtcgcg ggaaagcgtg gtgtattcgc cggaaacgcg
      241 ttcgcgaagc gtcagcagaa tatctttgaa ttcacggatc tctttagagg tcagctttct
      301 ggcggcaggc agagcgggcg ccggagcagc cggcttatga acgtgaaccg gaaggacttt
      361 tttatccgcc ggcttcacag cagccggttt ggcggcggtt ttcgctgccg cctttttggc
      421 cggagctttt ttaacggttt tttcgacggc ttttttagcc ggtgcttttt taacggcttt
      481 tgccgccgtt tttttcttcg aaaccttttt tgcagccatt cgtaaagccc cttccgttat
      541 ccaaaaatac catatcagcg gaaaaatgag gggtgctttt agcacggcgt tgacaagagt
      601 gtcaaccggc atcccttcct atttccggcc tatttcaggc aatcgtcagc ccgtcccacg
      661 gaacctcaat cccggccggc agcgcggcct gaagcgtgtc atgatccgaa tgatgcgtca
      721 ggtgggtcag aaacgaccgt tttgcgccga atcgctgcaa atgctctacg cattcttcga
      781 ttgaaaaatg agtcgcatgc cttccgggac gaagcccgtc gagaatcatc acatccaggt
      841 tttccagccg gctgaaactt tccggaggga tgcctgagca gtcgggaatg tacgccagcc
      901 gtttgccatc cgcttcaacc agaaagccgt aaacgtcgtc ctgtccgtgt tttaccggaa
      961 ccggcgtcac ccgcgcaccg ccggcttcca ccggcgcgga catccctgta aagaccacgc
     1021 gcggcaccgc gccgaacgag tggcttggct tgtccacata atcaaacttc gtgcgcatcg
     1081 aggcgatggt ttccggagaa ccgtaaacgg gaagatgagc ttcctgcagc tcgctgaagc
     1141 ggcgaacgtc gtcgaacccg aaaatatggt cggcatgcgg atgggtgatg aaaaccgcat
     1201 caacccgctc caccccgaac gtcagaacct gatcgcgcag atcgggcggc gtgtcgaaca
     1261 gcagatgctt tccgcccgcc tggacataaa gactgcaccg gcggcgcttg ttcttcgggt
     1321 tgtccgaccg gcagaccgca cagcggcatc cgatcatcgg aacgccgtgc gaggttcccg
     1381 tgcctaggaa agtaattctc atgaaagccg gttcttgtag tcctcgtaat gaaactcgcg
     1441 gacgatttcg acgttgtcgc cgcgctgaat ggcgatggac ggatgcttga ttccgttgaa
     1501 cattgtggtc ttcaccatcg tgtagtgcgc catatcttca aacaccagcc ggtcgcccgg
     1561 cttgagcggc cggtcgaacg aatagtcgcc gatcacgtcg cccgccagac agctcattcc
     1621 cgccagccgg tacgtgtatg ctttttcgcc cgcctgtccc gctccggtaa tagccgggcg
     1681 gtacggcatt tccagcacgt ccggcatatg gcaggtgcag gaacagtcga gaatcgcgat
     1741 ctcgctggca ttttgaacca catcgagcac agtggtgatc agcacgccgg tgttcagcgc
     1801 aaccgcctcg cccggctcca gaatcacctg tacgccgtac ttcgctttaa aatcgttgat
     1861 cagcttgcag agcagatcga tgtcgtagtc ggcgcgagta atgtggtgtc cgccgccgaa
     1921 gttcacccat tccatctgcg gcaggaattc accgaacttt tcttcgatgg ccttgagggt
     1981 aatctccagc gcgtcggcgt tctgctcgca aagcgtatgg aaatgcagtc cgctgatccc
     2041 ttcaaggctt tcgccggcga actgatcgcg cgtgatgccg aggcgcgact tcggcgcgca
     2101 gggatcatac atcggcgtcc ggcccgtcga gcattccggg ttgatgcgca atccaaactt
     2161 aggtggaacg ggcgctccgc gcacgttcaa acgcgccggg acggcgcgtt ccaccaaagg
     2221 acggaacttc ttccactggg cgaacgaatt gaaatcgatc tcatcgcaaa gaatcagcag
     2281 ttcgcggacg tcggcttcgc tgtaagccgc ggcaaaggcg tgaacctcgc cgtcgaactc
     2341 ttcgcggccg agccgggctt cccacggcga gctggcgcag acgccgtcga gcgttttgcg
     2401 cagcagcgga aacgtactcc acatcgcgaa tcctttgagt gccagcagga ttttacagcc
     2461 ggtgcgcttt ttcaccaaag cgagcgtatc gaggttgcgc tgaagcaaag cttcatcgac
     2521 gacaaaactg ggagttggaa ccgtttgaaa attcatgcgg cggttttagc gtcgggatgc
     2581 gctgattgca cgtcaatttt cttgtggggc agacattcct gtctgcccgt ttgcgcagac
     2641 aggaatgtct gcgccacaat cttaagcgtg gcggtaggtg atgcggccct tagtcaggtc
     2701 gtaggtggag atttcgacgg tcacgcggtc gccgatggaa atgcggatga agtttttacg
     2761 gattttgccg gagacgtgcg ccagcaccgt atgcccgtta tcaaacttta cgcggtacat
     2821 cgtgccggga agaacctcgg ttaccgtccc gtccaacgaa attaaactgt catcctgctt
     2881 cgccatacag tctcctgaca tcaaaatt
//
LOCUS       DAOPZO010000180         2887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_47926_length_2887_cov_6.897952, whole genome shotgun sequence.
ACCESSION   DAOPZO010000180 DAOPZO010000000
VERSION     DAOPZO010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47926_length_2887_cov_6.897952"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..311)
                     /locus_tag="PKI68_08825"
     CDS             complement(24..311)
                     /locus_tag="PKI68_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53833.1"
                     /translation="MAAEKKTTKKTVKKATVKKASVKPAAPKAAEPKVAVRKAPAPKA
                     PASKAVVSHEKFYSMVSEAAYFAAQNDRNRKGSVEYWIEAEAALRAKYDIA"
     gene            455..>1212
                     /gene="argS"
                     /locus_tag="PKI68_08830"
     CDS             455..>1212
                     /gene="argS"
                     /locus_tag="PKI68_08830"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNX53834.1"
                     /translation="MKKETASLETQLCAWCEQTFAACFPGVDLSSVPLAVTPSKNEQF
                     GDCQCEAAMKLAKELKTNPRAVAQQFVDHAVLPDYVEKIDIAGPGFINIRLKTVALAA
                     QLEAMLADPKLGLPDLGKNRTVIIDYSSPNVAKPMHIGHIRSTVIGNALDRLYRALGY
                     HVIADNHLGDWGTQFGLIILGYREFADAEAIKTAPVEELERIYVKSYNMSKEDETWRD
                     RAKAELVKLQQGDKENRALWEKFVELTLGEFNRI"
     assembly_gap    1213..1312
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1313..2056
                     /gene="argS"
                     /locus_tag="PKI68_08835"
     CDS             <1313..2056
                     /gene="argS"
                     /locus_tag="PKI68_08835"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF017555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNX53835.1"
                     /translation="FKPAEIIYVTDERQQLHFKQFFHICKKLGVPTKLTHVWFGLMRL
                     PEATFSTREGNVIKLEALLNEAEARALEVVKASSPEMDEAQQREIARAVGIGAIKYTD
                     LSQNPQSLVTFTWEKALAMDGNSGPYLQYAYARIASVRDKYAAQFPGKNPDAYPVVLG
                     EEIERRLALKLTRFPAAVVSAASLDRPSVMADYLFDLSQAYSSFYQNVPFLKANEGVR
                     ESRIRICALTAAILKKGLALLGIETPERI"
     gene            2153..2374
                     /locus_tag="PKI68_08840"
     CDS             2153..2374
                     /locus_tag="PKI68_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53836.1"
                     /translation="MRIEQWPVQHSHPHMALHMPHPMHWMHEHQAAGALMAIGLLILL
                     IAGIMLLAFRLGELSVSGTSSPLQAPLYL"
     gene            2539..>2887
                     /locus_tag="PKI68_08845"
     CDS             2539..>2887
                     /locus_tag="PKI68_08845"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNX53837.1"
                     /translation="MTNADKIIGQLKTLGNRTAAEHAQRFFKTGPGQYGEGDIFLGIK
                     VPVLHRLAKEHQAIALNEAVELLQSPLHEVRLLALFIMVLQYKRGGNEAAIYRAYLAN
                     THRINNWDLVDSSA"
BASE COUNT          627 a          883 c          730 g          547 t
ORIGIN      
        1 gggtctcgaa cggtgcgccg ggatcaggcg atatcgtatt tggcgcgcag ggcggcctcg
       61 gcttcgatcc agtattccac ggaccctttg cggttgcggt cgttctgggc ggcaaaatag
      121 gccgcctcgc tgaccatgga gtagaacttt tcatgggaga caacggcctt tgatgccggc
      181 gctttcgggg ccggagcttt ccggaccgca accttcggtt cagcggcctt cggcgcggcg
      241 ggtttgacgg aggccttttt tacagtcgct ttctttacgg tcttctttgt tgttttcttt
      301 tcggcagcca ttttttatct cctccaacaa ttctttttgc accacggcta cgaactctaa
      361 aattccggcc cctgaatgca agaacgaaaa ggcgctttaa ctggattccg aaatatggtc
      421 ttatccgcgc tgctttcagc ccggaaatta cagaatgaaa aaagaaacag cctctctgga
      481 aacacagctc tgcgcctggt gcgaacaaac cttcgccgcc tgctttcccg gcgttgacct
      541 gagcagcgtt ccgctggccg tcacgccctc caaaaacgag cagttcggcg actgccagtg
      601 cgaagccgcc atgaagctgg ccaaggaact gaaaaccaac ccgcgcgccg ttgcccagca
      661 gtttgttgat cacgccgttc tgccggacta cgtggagaaa atcgatatcg ccgggccggg
      721 cttcatcaat atccgcctca aaaccgtcgc gctggctgcg cagctcgagg cgatgctggc
      781 cgacccgaaa ctcggcctgc cggatctcgg caagaaccgg accgtgatca tcgactactc
      841 cagtccgaat gtcgccaagc cgatgcatat cggccacatc cgctccaccg tgatcggcaa
      901 cgccctcgac cgcctttacc gcgccctcgg ctaccacgtc atcgccgaca atcacctcgg
      961 cgactggggc acgcagttcg gcctgatcat tctcggctac cgcgaattcg ccgacgccga
     1021 agccatcaaa accgcgccgg tcgaagagct cgaacgcatc tacgtcaaaa gctacaacat
     1081 gtccaaagag gacgaaacct ggcgcgaccg cgccaaagcc gaactcgtca aactccagca
     1141 gggcgacaaa gaaaaccgcg cgctctggga aaaatttgtc gaactgaccc tcggcgaatt
     1201 caaccgcatc tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttcaagcc
     1321 ggcggaaatc atctacgtca ccgacgagcg ccagcagctg cacttcaagc agttcttcca
     1381 catctgcaag aagctcggcg tgccgaccaa gctcacccac gtctggttcg gcctgatgcg
     1441 cctgccggaa gccaccttct cgacccgcga aggcaacgtc atcaaactcg aagcgctgct
     1501 caacgaagcg gaagcccgcg cgctcgaagt tgtcaaagcc tccagccccg agatggacga
     1561 agcccagcag cgcgaaattg cccgcgcggt cggcatcggc gcgatcaaat acaccgacct
     1621 cagccagaac ccgcagagcc tggtcacctt cacctgggaa aaagcgctcg ccatggacgg
     1681 caactccggg ccgtatcttc agtatgccta cgcccgcatc gccagcgtcc gcgacaaata
     1741 cgccgcgcag ttccccggca agaatccgga cgcgtatccg gtcgtcctcg gcgaagagat
     1801 tgagcggcgc cttgcgctca agctcacgcg cttcccggcg gctgtcgttt ccgccgccag
     1861 cctcgaccgg cccagcgtga tggccgacta cctcttcgac ctttcacagg cctacagctc
     1921 gttctaccag aacgttccgt tcctgaaagc caacgaaggc gtccgtgaaa gccgcatccg
     1981 catctgcgct ctcaccgccg cgatcctgaa aaaaggactc gcccttctcg gcatcgaaac
     2041 gcccgagcgg atttagaatt caaccctttc gccggtcatc ggcagaatta aaaaacgtgc
     2101 ttttctgcgg ccaaaatggt atacagaaga tgcacggaag ggggtgaata ccatgagaat
     2161 cgaacaatgg cctgtacagc attctcatcc gcatatggca ctgcatatgc cgcatccgat
     2221 gcattggatg catgaacatc aggcggccgg tgctttgatg gcaatcggct tgctgatcct
     2281 gctgatagcg ggaataatgc tcttagcttt tcgcttaggt gagctctcag tcagcggcac
     2341 atcttcgccg ttgcaggcgc cgttatatct gtaaagttcg gcttcaggca agcggaggcg
     2401 tgaaaggtca atgccgaccc gcgatgaaca aacgccctcc gctcctgact taaaaatttg
     2461 cgggggcttt tttatatggc gttcttcctc cataaaagcg gcggactaat cgtgtgaatc
     2521 cgtggttaaa tgctccccat gaccaacgca gacaaaatca tcggacagtt aaagacgcta
     2581 ggaaaccgga ccgccgcaga acacgcgcaa cggtttttca aaaccggccc cggacagtac
     2641 ggcgaaggcg acatcttcct cggcatcaag gttccggttc tgcacaggct cgccaaagaa
     2701 catcaggcaa ttgccctgaa tgaagccgtc gagctgttgc agtcgccact gcacgaagtc
     2761 cgcctgctgg cgctgttcat catggtcttg cagtacaaac gcggcggaaa cgaagcggcg
     2821 atttaccgcg cctacctcgc caacacgcac cgcatcaaca actgggatct ggtggacagc
     2881 tccgcgg
//
LOCUS       DAOPZO010000181         2867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_48454_length_2867_cov_11.027027, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000181 DAOPZO010000000
VERSION     DAOPZO010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48454_length_2867_cov_11.027027"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PKI68_08850"
     CDS             <1..262
                     /locus_tag="PKI68_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53838.1"
                     /translation="TRLPMLRCANTGITCSIDSSGRIRQTLEPRTEGIQITETMPADP
                     AREATFYVRFGDLFAQACLAGSVALFIVLFITRRKSEACLKN"
     gene            247..1368
                     /gene="prfB"
                     /locus_tag="PKI68_08855"
     CDS             247..1368
                     /gene="prfB"
                     /locus_tag="PKI68_08855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNX53839.1"
                     /translation="MLEELKEKMTTVRSRMEEMRGYLGINEKIDELNRLEAETASPGF
                     WNDQNRTAANIAATKAAKAVLGPFQQIEASLDDAGVLLELAEAEDEAGQQAAADEIEQ
                     MLSVCESSFRDLEMQSLLGGPMDSKNAYLSLHAGAGGTESCDWADMLYRMYRRYAERN
                     GFTVDVLDIQPGEEAGIKSVALQVSGNYAYGYLKGERGVHRLVRISPFDSAARRHTSF
                     VALDVTAEIDDDVKVEIEEKDLRIDTYRSSGSGGQHVNTTDSAVRIVHIPSGIIVQCQ
                     AERSQHKNRDKAMKMLRAKVYEYELDKQKQAAEQFYGAKGEIAWGHQIRSYVMQPYTM
                     VKDHRTDAQTGNVQSVLDGNLALFIEAYLKTKQKEAAGG"
     gene            1365..2183
                     /gene="trpC"
                     /locus_tag="PKI68_08860"
     CDS             1365..2183
                     /gene="trpC"
                     /locus_tag="PKI68_08860"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNX53840.1"
                     /translation="MNILEEIIAGKRREIETPGYFRTLEKVIENLPHVGTACTACDGE
                     PARQPVPDFIAALKSAPVGLIAEVKRKSPSAGPIRTPFHPAEIARAYETAGAQAISCL
                     MDSVYFGGGEEQWTAVRAATALPMLYKEFVIDPRQIFHAEALGASAALLIVAALEDAE
                     LKKFIRLTEASGMTPLVEVHTEEEMKRAVSAGAECIGINNRNLKTFETRIETTLALRK
                     LAPKDAVLISESGIRTAEDVRMLQDAGISAVLVGESLLRQADIGAAVRSLMNLS"
     gene            2236..2311
                     /locus_tag="PKI68_08865"
     tRNA            2236..2311
                     /locus_tag="PKI68_08865"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2269..2271,aa:Asp,seq:gtc)
     gene            2507..2761
                     /locus_tag="PKI68_08870"
     CDS             2507..2761
                     /locus_tag="PKI68_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53841.1"
                     /translation="MGDRVPMFKLRPYRGLLLAALFVLIVGGLLSAYFFTEDAYCTGS
                     RRGLFVLIVTLILTFFLIVVAFSRYGFRHLWHHRPGYKRG"
BASE COUNT          636 a          800 c          815 g          616 t
ORIGIN      
        1 aacccgcctg ccgatgctgc gctgcgccaa taccggcatc acctgctcga ttgattcatc
       61 cggccgcatc cggcagacgc tggaaccgcg caccgaagga attcaaatta cagaaacaat
      121 gccggccgat ccggcgcgcg aggcgacctt ctacgtccgc ttcggcgacc ttttcgcgca
      181 ggcttgcctg gccggaagcg ttgcgctatt tatcgttctc tttataacac gccggaaaag
      241 tgaagcatgc ttgaagaact gaaagagaaa atgacgacgg tccgctcgcg catggaggaa
      301 atgcgcggct atctcggcat taatgaaaag attgacgaac tcaaccgcct cgaagcggag
      361 acggcctcgc ccggattctg gaacgatcag aaccggaccg ccgccaatat cgccgccacg
      421 aaagcggcga aagccgtgct gggcccgttt cagcaaattg aagcctcgct cgacgatgcc
      481 ggagtgctgc tggaacttgc cgaagcggaa gatgaagccg ggcagcaggc ggccgcggat
      541 gaaattgaac agatgctttc cgtctgcgaa agcagtttcc gggatttgga aatgcagtcg
      601 ctgctcggcg gccccatgga ttcaaagaat gcctatctca gtctgcacgc cggtgcgggc
      661 ggcaccgagt cgtgcgactg ggccgatatg ctctaccgca tgtaccgccg ttatgccgaa
      721 cgcaacggat ttaccgtcga cgtgctcgat atacagccgg gagaagaggc cggcatcaag
      781 agcgtggccc ttcaggtttc cggaaattat gcctacggct atttgaaggg cgaacgcggc
      841 gttcaccggc tggtgcgtat cagccccttt gactccgccg cccgccggca tacctcgttc
      901 gtcgcgctcg acgtcaccgc agaaatcgac gatgacgtga aggttgagat cgaagaaaaa
      961 gatctgcgca tcgacaccta ccgttccagc ggatcgggcg gacagcacgt caacacgacc
     1021 gattccgccg tgcgcatcgt gcatattccg agcggaatca ttgtccagtg tcaggcggaa
     1081 cgctcgcagc acaagaaccg cgacaaggcc atgaagatgc ttcgcgccaa agtctacgag
     1141 tacgaactcg acaaacagaa gcaggcggcg gaacagtttt acggagccaa aggtgaaatt
     1201 gcctggggcc accagatccg ttcctatgtc atgcagccct acacgatggt gaaagatcac
     1261 cgcaccgacg cgcagaccgg caatgtacaa agcgtactcg acggcaatct ggccctgttt
     1321 atcgaagcct atctcaagac gaaacagaaa gaggcggccg gcggatgaat attttagagg
     1381 aaatcatcgc cggcaaacgg cgggagattg aaacgcccgg atatttccgg acgcttgaaa
     1441 aagtgatcga gaatcttccg cacgtcggaa cggcgtgtac cgcctgtgac ggagaacctg
     1501 cgcggcagcc tgttccggac tttattgccg cgctgaaatc tgcgccggtc ggcctgattg
     1561 ccgaagtgaa acggaaatcg ccgtcagccg gaccgatccg tacgccgttt catccggctg
     1621 aaatcgcgcg cgcttatgaa accgcaggtg cgcaggcgat ctcgtgcctg atggattccg
     1681 tctatttcgg cggcggcgaa gaacaatgga ctgcggtacg cgcggcgacg gctctgccga
     1741 tgctctataa ggaattcgtt atcgatccgc gccagatttt tcacgccgaa gcactcggcg
     1801 catcggcggc tctgctgatc gtcgcggcgc ttgaggatgc tgagctgaaa aagtttatcc
     1861 ggctgacaga ggcttccggc atgacgccgc tggtcgaagt gcatacagaa gaagaaatga
     1921 aacgggcggt ttccgccggt gcggagtgca tcggcatcaa caaccgcaac ctcaagacct
     1981 tcgaaacgag aattgaaacc acactggcgc tccgcaagct ggccccgaaa gacgctgtgc
     2041 tgatcagtga aagcggtatt cgcacggcgg aagacgttcg gatgcttcag gatgcgggaa
     2101 tttccgccgt tctggtgggc gagagtctgc tccggcaggc cgatatcgga gccgcagtcc
     2161 ggtctctgat gaacttgtcg tagtttgcgc ttgaaataca aagtccgccg actattctcc
     2221 ttgtctcttt cttgcggggg cgtagctcaa tggttagagt gccagcctgt cacgctggat
     2281 gttgcgggtt cgagtcccgt cgctcccgcc acttctaacg gatttcaaag ctgagaaatc
     2341 cgtttgtccg gctctccccc tttttcaaaa aaacaccttg ttttcatgag gattttcacc
     2401 gttcatgaaa aagtttctcc gccggggaat aattaatgct ttatttgccg caaaggtctc
     2461 aatagagtcc aaaacagcgt aacaaaacag gtgaatgatg attttaatgg gcgacagagt
     2521 tccgatgttt aagttgcggc cctatcgcgg gcttttgctg gcggcgcttt ttgtgcttat
     2581 tgtcggcggc ctgctttccg cttatttttt cacagaagac gcctactgca ccggcagccg
     2641 ccgcgggctg tttgtcctga tcgttacact gatccttact tttttcctga tcgttgtagc
     2701 cttttcgcgc tacggcttcc ggcatctatg gcatcatcgc cccggttata agcgcggctg
     2761 agggtttgac tttgccgtta cttttcgcat aatcccgcac tcttttccca gcgccggcgt
     2821 agctcagtgg tagagcagct gatttgtaat cagctggtcg ggggttc
//
LOCUS       DAOPZO010000182         2851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_48881_length_2851_cov_10.330114, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000182 DAOPZO010000000
VERSION     DAOPZO010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48881_length_2851_cov_10.330114"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PKI68_08875"
     CDS             complement(<1..333)
                     /locus_tag="PKI68_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNX53842.1"
                     /translation="MIVTTKELFKHAYGKYAVGAYNINNAEQVMGLFRGCMESKAPFI
                     VQLSKGARSYTDKRMLEAMLRAADEIFPDAIFAVHLDHGDEKTCMDCIESGFYSSVMI
                     DASHESFDE"
     gene            435..1022
                     /locus_tag="PKI68_08880"
     CDS             435..1022
                     /locus_tag="PKI68_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53843.1"
                     /translation="MKASCYLTPMSADPFHLNRPVHLARRDVFFEKAVARIRKLKNQD
                     QPAEQCKTAEEALFLLLEARLCAEAANLSEKTSTQNRLFMDFMDTAVDNTRSITSLLR
                     RQISVNVPHNPLDLFLGSGDTGSKMSGHYRRCAAHILKGLLLILQKAEKPFRDLRQTS
                     VAKMTAEDKIRYEKAYSHFEELADSEAEKAVAEAL"
     gene            complement(1019..>2851)
                     /gene="mutL"
                     /locus_tag="PKI68_08885"
     CDS             complement(1019..>2851)
                     /gene="mutL"
                     /locus_tag="PKI68_08885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNX53844.1"
                     /translation="ESASIRVHPRLKIFRTSAKAANVPPMNQHIQMLPDHVINKIAAG
                     EVIERPASVMKELVENAIDAGATQVDVEVVQGGMQLIAVSDNGSGMNRDNALLSIERH
                     ATSKIRTAEEVEAVATLGFRGEALSSIAAVSRFTLITRPAAELSGTQILVAGGKLQDV
                     LDAGCPPGTRFEIRNLFFNVPARRKFLRTEATELAQIRQLFAVYALAHPETGLTFTSD
                     GREVYNLPGGSSLADRITALYSPAFLSHLRKLDFEADGVKISGFAGLPQTGRSDRSDQ
                     YVFINGRPASAPIIYHALNEAYHAVLARGRYPSAFLFIETPPAQVDVNVHPAKKEVRF
                     RHPAAVRDAVIAAVQKALALEVPEAERREPAFKFDAPRPAPRVEKQHEFEQVSGGACR
                     VAAPDVPLANTETPKAQSLKPSSSPWKWCRVLGQAGGLYAVLETEEGIVLMDPQAAHE
                     RVLFEKFMHDLTAGKPSRQGLLTPEAIELMPADAEALRRHLPVLDEMGFGISAFGQDT
                     FIVDALPVHLQNGSPIVILSGIARALEKSGAASRATREALRETVAQAACRTSIKARDT
                     LPAAAIERLVEDLAKTEMPYTCPHGRPTLIFTSFTELNRKFGRA"
BASE COUNT          597 a          886 c          793 g          575 t
ORIGIN      
        1 ttcgtcgaac gactcatggc tggcgtcgat catgacggag ctgtagaagc cgctttcgat
       61 acagtccata caggtctttt cgtcgccgtg gtcgagatgc accgcgaaaa tggcatccgg
      121 gaaaatttca tccgctgcgc gcagcatggc ttcgagcatg cgtttgtcgg tgtaggaacg
      181 ggcgcccttg gagagctgca cgataaacgg tgctttgctt tccatgcagc cgcggaacag
      241 acccatcacc tgctcggcgt tattgatatt ataagcaccg acggcgtatt tgccgtaggc
      301 atgcttaaac agttcttttg tcgttacaat catcagtttc tcccttacgt ggttccggtt
      361 aaattgcgcg cgaattttag ctgagcgacc cccgattacc accttaaact gcggtttttt
      421 cagaggttgc cgccgtgaaa gcgtcctgtt atcttacgcc catgagcgcc gaccctttcc
      481 atctgaaccg gccagtccac cttgcccgcc gcgacgtttt ttttgaaaaa gccgtggcgc
      541 gcattcgaaa actcaagaac caggatcagc cggcggaaca gtgtaaaacc gccgaagaag
      601 cgctgtttct gctgctcgaa gcccgcctct gcgccgaagc cgccaatctg tcggaaaaaa
      661 cctccacgca aaaccggctt ttcatggatt ttatggatac ggcggtcgac aatacccggt
      721 ccatcaccag cctgctgcgt cgccagatca gcgtgaatgt cccgcacaat ccgctcgacc
      781 tgtttctcgg ctccggcgat accggctcga aaatgagcgg tcattaccgc cggtgcgctg
      841 cgcacatcct gaaaggtctg cttctgattc tgcagaaagc ggaaaaaccg tttcgcgacc
      901 tccggcagac cagtgtcgcc aaaatgaccg ccgaagacaa aatccgctac gaaaaagcct
      961 atagccattt cgaagaactg gcagacagcg aagcggaaaa agcggttgca gaggctctct
     1021 aagcgcggcc gaacttgcgg ttcagctccg taaagctggt aaagatcagt gtcgggcgcc
     1081 cgtgcggaca ggtatacggc atttccgtct tcgccagatc ctccaccagc cgctcaatcg
     1141 ccgccgccgg aagagtatcc ctggctttaa tcgacgtgcg gcaggccgcc tgcgccaccg
     1201 tttcgcgcag cgcttcgcgc gttgcccttg acgccgcgcc gcttttttcc agcgcacgtg
     1261 caatgccgct cagaatgaca atcggcgaac cgttctgcag atgaaccgga agcgcatcga
     1321 caataaacgt atcctgcccg aatgcggaaa tgccgaagcc catttcatcc agcaccggaa
     1381 gatggcggcg gagcgcctcg gcatccgccg gcatcagctc gatcgcctcc ggcgtcagca
     1441 ggccctggcg cgacggcttc ccggcggtca gatcgtgcat aaatttttca aacagcaccc
     1501 gttcgtgcgc cgcctgcgga tccatcagca caatgccctc ttctgtttcc agtacagcat
     1561 aaagtccgcc cgcctgaccc agcacacggc accatttcca aggggatgag gaaggcttta
     1621 ggctttgggc tttaggcgtt tcggtattag ccagcggcac atccggagcc gccacccgac
     1681 acgcgccacc cgacacttgt tcaaattcat gctgcttttc gacacgcggc gcggggcgcg
     1741 gagcatcaaa tttaaaggcc ggttcgcggc gctcggcctc cggcacttcc agcgccagcg
     1801 ctttctgcac cgccgcaatt acggcgtcgc gcacagcggc aggatggcgg aaccggactt
     1861 ccttcttggc tggatgcaca ttcacatcca cctgagccgg aggcgtttcg ataaaaagaa
     1921 aagccgacgg ataccggccg cgcgccagca ccgcgtgata ggcctcgttc agcgcgtggt
     1981 aaattatcgg cgcagaagcc ggacggccgt tgatgaaaac atactgatcc gagcggtcgg
     2041 aacggccggt ttgcggaagt ccggcgaaac ccgaaatctt cacgccgtcc gcttcaaaat
     2101 cgagtttgcg cagatggctc agaaacgccg gactgtacag cgcggtaatg cggtcggcca
     2161 gcgacgagcc gccgggcaga ttgtaaactt cgcgtccgtc ggacgtgaac gtaagccccg
     2221 tttcgggatg cgccagcgca tagaccgcaa acagctggcg gatctgtgcc agctcagttg
     2281 cctccgtgcg cagaaacttg cgccgcgccg gcacgttgaa aaacagattg cggatttcga
     2341 accgcgtacc gggcggacag cccgcatcca gtacatcttg cagcttgccg ccggcaacaa
     2401 ggatctgcgt gccgctcagt tctgcggcgg gccgcgtaat cagcgtaaag cgcgacacgg
     2461 cggcgatgga cgaaagcgcc tcgccgcgga accccagcgt cgccaccgct tccacctctt
     2521 cggctgtgcg gatcttgctt gtcgcgtgac gttcgatgga aagcagcgcg ttgtcgcggt
     2581 tcatgccgga accgttgtcg gaaacggcaa tcagctgcat cccgccctgc acgacctcga
     2641 catccacctg cgtcgcgccg gcgtcgattg cgttttcgac cagctccttc attacggaag
     2701 cgggccgctc gatcacctct cccgccgcga tcttattgat cacatgatcc gggagcatct
     2761 ggatgtgctg attcatcggc gggacattag ccgcttttgc cgaagtccgg aagattttta
     2821 accgcggatg aacacgaatg gacgcggatt c
//
LOCUS       DAOPZO010000183         2797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_50381_length_2797_cov_10.635667, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000183 DAOPZO010000000
VERSION     DAOPZO010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50381_length_2797_cov_10.635667"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..637
                     /gene="lspA"
                     /locus_tag="PKI68_08890"
     CDS             155..637
                     /gene="lspA"
                     /locus_tag="PKI68_08890"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNX53845.1"
                     /translation="MLVLILGLIVVLLDQLTKLWVRGSCIYGGEPQSVIPGFFNLVYV
                     RNEGAAWGMFGGQMPVLIILSITVLVLLAVYHRKVLNPTLDHRIALGLMVGGICGNLI
                     DRVRVGWVTDFLDFYLGSYHWPSFNVADSAICIAVGIYLLSSFWHARHPLKNAANETK
                     "
     gene            624..1532
                     /gene="miaA"
                     /locus_tag="PKI68_08895"
     CDS             624..1532
                     /gene="miaA"
                     /locus_tag="PKI68_08895"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNX53846.1"
                     /translation="MKRNSPSAFVLVGPTASGKSAVAQRLAEQTGTSIISADSMNIYR
                     GMDIGTAKPSSAERARVQTWGVDLADPAEPFSVGDWLRAVRPAFDTAQPPIVAGGTGL
                     YVKCLLQGLDELPPADEALRERTEKMTLTELQAEARQAAPEAYEMLADKENPRRLVRL
                     IEQGGQSRTTWQNELPVVVGLHVERDVLHRRIAERVDKMYAGGLLDEARRLLGLELSA
                     TAQHAIGYAEAFAVLRGECSAAEAKEKTIVRTRQLSKRQMTWFRHQLNVEWIETACCS
                     DVGQLADKVYNIWRERGPVPLAGVCL"
     gene            1529..>2797
                     /gene="hflX"
                     /locus_tag="PKI68_08900"
     CDS             1529..>2797
                     /gene="hflX"
                     /locus_tag="PKI68_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNX53847.1"
                     /translation="MKEMKTTVPHAQERALLVGVMVRGRNDEWDVKDTLDELAQLTES
                     AGAEVLGRMLCKQSKVHAAHFIGKGKVEELAQMAKETGANLVIFDEDLSPAQGRNLEE
                     TLEVRVLDRTQLILDIFAKRARTREGCLQVQLAQHRYLLPRLRRMWTHLERQAGGIGL
                     KGPGETQIEMDRRSIQNLIRTLAQELELVRTRRAEQRRGRQRHGWALVSIVGYTNAGK
                     STLLNRLADADIYAQDQLFATLDPTTRQVELPNREPCLMTDTVGFIRKLPHHLVDSFK
                     ATLEEVVEADLVLHVIDCAHPQVEQQIDAVHAVLQEIGADDKPVLYVLNKIDVEEGRH
                     AASRLARTLGRSAAVSAVTGEGVETLLNELADCLKGDKLVMKLAIPLSEGRLLALLKQ
                     SATVLEEKYEGEFAELLVRVPAQLVPRCEPY"
BASE COUNT          561 a          836 c          864 g          536 t
ORIGIN      
        1 cggtatctgc gaactgaccg gccagccgat caatatcgaa cgtcttcagg ctctgccgta
       61 tgtgcgctat accgtgcgtg cgcagtccga actcgaaaaa ggccgcgccc gctaccgtcc
      121 gttcggcagc accatgcgca gctaggagaa ccccatgctg gtactcattc tgggtctgat
      181 cgttgttctg ctcgatcagt tgaccaagct gtgggtgcgc ggttcctgca tctacggcgg
      241 tgagccgcag tctgtcatcc cgggtttttt caacctggtc tacgtccgca acgaaggcgc
      301 ggcgtgggga atgttcggcg gacagatgcc ggtgctgatt atcctctcga tcaccgtcct
      361 cgttctgctg gccgtctatc accgcaaagt gctcaacccg actcttgacc atcgcatcgc
      421 gctgggtctg atggtcggcg gcatttgcgg gaacctgatc gaccgcgttc gcgtcggctg
      481 ggttaccgac ttcctcgatt tctacctcgg ttcgtatcac tggccgtcgt tcaatgtcgc
      541 cgattcagcg atttgcatcg ccgtcggcat ttatctgctt tcttcgtttt ggcatgcccg
      601 ccatcctctg aagaatgctg ctaatgaaac gaaatagtcc cagcgcattc gtcctcgtcg
      661 ggccgacggc atcgggaaaa agcgccgtcg cccagcggct ggctgaacag accggtacgt
      721 ccatcatctc cgccgactcg atgaacattt accgaggaat ggatatcggc acggccaaac
      781 catcgtccgc cgaacgggcc cgcgttcaaa cctggggggt cgatcttgcc gatccggcgg
      841 aaccgttcag tgtcggcgac tggcttcgcg ccgtccgccc ggcctttgac actgcgcagc
      901 cgccgatcgt cgcgggcgga accggacttt acgtcaaatg cctgctgcaa ggcctcgatg
      961 aactgccgcc ggcggacgaa gcgctgcgtg aacgcacgga aaaaatgacg ctgaccgaac
     1021 ttcaggctga agcccggcag gccgcacccg aagcttacga aatgcttgcc gacaaagaaa
     1081 atccccgccg cctcgtccgg ttgatcgaac agggcggaca gagccgtacc acatggcaga
     1141 acgaactgcc ggttgtagtc ggcctgcatg ttgaacgcga cgtgctgcac cggcggatcg
     1201 ccgaacgggt cgataaaatg tatgcgggcg ggttgctcga cgaagcccgc agactgctgg
     1261 gcctggaact ttcggccacc gcacagcacg ccatcggcta cgccgaagcc ttcgcggttc
     1321 tgcgcggtga atgttccgcg gcggaagcga aagaaaaaac catcgtccgc acccgccagc
     1381 tctccaagcg ccagatgacc tggttccgcc atcagctcaa cgtcgagtgg attgaaaccg
     1441 cctgctgttc agacgttgga caacttgcgg ataaggttta caatatctgg agagagcgcg
     1501 ggccggtgcc gctggcggga gtctgcttat gaaagagatg aaaaccacag ttcctcatgc
     1561 gcaggagcgg gcgctgctgg tcggcgtgat ggtgcgcggg cggaatgatg agtgggatgt
     1621 caaagacacg ctcgacgagt tggcgcagct gaccgaaagc gcgggcgcgg aagtgctggg
     1681 tcggatgctt tgcaaacagt ctaaagttca tgccgcccat ttcatcggca aaggcaaggt
     1741 ggaagagctg gcgcagatgg cgaaagagac cggcgccaat ctggtgattt tcgatgaaga
     1801 tctttcgccg gcgcagggcc gcaatctgga agaaactctg gaagtccgcg tgctcgaccg
     1861 cacacagctg attctcgata ttttcgccaa gcgggcgcgg acgcgcgaag gctgtctgca
     1921 agtgcagctg gcccagcacc gctaccttct gccccgcctg cgccggatgt ggacgcattt
     1981 ggagcggcag gccggcggta tcggcctgaa agggccgggc gaaacgcaga tcgagatgga
     2041 ccgccgcagt attcagaatc tgatccgcac gctggcgcag gagctggagc tggtacgtac
     2101 acgccgcgcc gaacagcggc gcggacgcca gcgccacggc tgggcgctgg tttccatcgt
     2161 cggctacacc aacgccggaa aatccacact gctcaaccgg ctggcggacg cggatattta
     2221 tgcgcaggat cagctcttcg ccacgcttga cccgacgacc cggcaggtcg aactgccgaa
     2281 ccgcgagccg tgtctgatga ccgacacggt cggctttatc cgcaagctgc cgcaccacct
     2341 cgttgattcc tttaaagcca cgctcgaaga agtcgtcgaa gccgatctgg ttttgcacgt
     2401 catcgactgc gcgcatccgc aggtcgaaca gcagattgat gcggttcacg ccgtgctgca
     2461 ggaaatcggt gcggatgata agccggtgct gtatgtgctg aataaaattg atgtcgaaga
     2521 ggggcggcac gccgccagcc ggctggcgcg aaccctcgga cgctcggcgg ccgtttcggc
     2581 ggttacgggc gaaggggttg aaaccctgct gaacgaactg gccgactgtc tgaaaggcga
     2641 caaactggta atgaagctgg cgattcctct cagcgaaggc cgcctgctgg cgctgctgaa
     2701 acagtcggcg acggtgctgg aagaaaagta tgaaggtgaa tttgccgaac tgcttgtccg
     2761 cgtacccgcc cagctcgtcc cgcgctgcga gccgtat
//
LOCUS       DAOPZO010000184         2709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_52826_length_2709_cov_8.449133, whole genome shotgun sequence.
ACCESSION   DAOPZO010000184 DAOPZO010000000
VERSION     DAOPZO010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52826_length_2709_cov_8.449133"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1481)
                     /locus_tag="PKI68_08905"
     CDS             complement(<1..1481)
                     /locus_tag="PKI68_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNX53848.1"
                     /translation="MKRPNWLHILAPLAVSGSCATSFAAPTPPSNPNIIIIFADDQGY
                     GDVSCYNSESKIQTPFIDRMAAEGMRFTDAHASSAVCTPSRYGLLTGRYCWRSGFKAG
                     VLYGYSPSILKPPRETIASILKKKGYRTACIGKWHLGLDMATTDGKPIDQHETTPSNV
                     DMAGPLRNGPTDLGFDYFFGCSAAWDMPPYVWIENNRFTITTNFESAAKNEFGRRGVK
                     APGMTAAVAMPTFTQKAVAYIDASAKEKSGQPFFLYFPLTAPHVPVAPNPAFKGRSQA
                     GDYGDFVTEVDDTVGQILAALVRNGLDSNTLVIFTSDNGPETQAIARKTQYNHFSAEK
                     FLGIKRDNWDGGHRIPFIARWPGQIPAGSVSGEIICLNDLMATAAALAGCELPPNAGE
                     DSYNIWPVLAGEKPAAPIREATVYHSSTGLFAIRQGRWKLLLHSGSGGNKEYQRDPAY
                     AVYYSKPVQLYDMTADPYETVNLAESHPEIVAQLTALLKQYVL"
     gene            1700..>2709
                     /gene="htpG"
                     /locus_tag="PKI68_08910"
     CDS             1700..>2709
                     /gene="htpG"
                     /locus_tag="PKI68_08910"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNX53849.1"
                     /translation="MAEKTMEFKTELSQLLHLITHSLYSHREVFLRELISNACDAIDK
                     IRFEALTRTELLEGGSDTDWKIELAVDKEAKTLTVSDNGIGMDEEALVRNLGTIAHSG
                     TKAFLARLKESGAQNNPELIGQFGVGFYSSFMVADKVTVESRMHGAPAVRWVSEGTGS
                     FTVSEGSRETRGTSVTLHLKEDAAEYLEEYRLRTLVKKFSDFVEHPVMLGGETVNTRK
                     AIWLRSKSEITAEEYNEFYQHISHDFNDPAEVIHYNAEGAIEFKALLFIPEKKPFDMM
                     MGHDPKAHLNLYVQRVFISNEFENLLPGYLRFVKGVVDAADLPLNVSREILQENPQLD
                     KIR"
BASE COUNT          508 a          745 c          877 g          579 t
ORIGIN      
        1 ttgagcacat actgtttcag cagcgcggtc agttgcgcga caatctccgg gtggctttcg
       61 gccaggttga ccgtttcgta cgggtcggcg gtcatgtcgt agagctggac cggtttgctg
      121 tagtagactg cgtaggccgg atcgcgctga tactctttgt ttccgcccga accggaatgc
      181 agcagcagtt tccaccgtcc ctggcggatg gcgaagaggc ctgtgctgga gtggtacacc
      241 gtggcttcgc ggatcggcgc ggccggtttt tcgccggcca gtacgggcca gatgttatag
      301 ctgtcttcac cggcgttcgg cggcagctca cagccggcca gcgcggccgc cgtggccatc
      361 aggtcgttca ggcagatgat ttctccgctg acgctgccgg ccggaatctg tccgggccag
      421 cgggcgatga acgggatccg gtggccgccg tcccagttgt cgcgtttgat gccgagaaac
      481 ttttcggcgc tgaagtggtt gtattgggtt ttgcgggcga tggcctgtgt ttcggggccg
      541 ttgtcgctgg tgaagatgac cagggtgttg gaatcgaggc cgttgcggac gagcgccgcg
      601 agaatctgcc cgaccgtgtc gtcgacttcg gtcacaaagt ccccgtagtc gccggcctgg
      661 ctgcggcctt taaaggccgg gttgggcgcg accggcacat ggggtgccgt cagcgggaaa
      721 tagaggaaga agggttggcc gctcttctct ttggcggagg catcaatata ggcgaccgcc
      781 ttctgtgtga atgtgggcat ggccacggct gcggtcatgc cgggcgcttt gaccccgcgc
      841 cggccgaatt catttttggc cgcgctttcg aaattcgtgg tgatggtgaa tcggttgttt
      901 tcgatccaga cgtagggcgg catgtcccag gcggcggagc agccgaagaa ataatcgaat
      961 ccgaggtcgg tgggcccgtt gcggaggggg ccggccatgt cgacgtttga aggggttgtc
     1021 tcgtgctgat cgatcggctt gccgtccgtt gtggccatat ccagacccag gtgccatttg
     1081 ccgatgcagg ccgtgcggta gcctttcttc ttcagtatgg aggcgatggt ttcgcgcggg
     1141 ggtttgagga tggagggaga gtagccgtaa agaactccgg ctttgaatcc ggagcgccag
     1201 cagtagcgcc cggtcagcag cccgtagcgg ctgggcgtgc agacggcgct gctggcgtgc
     1261 gcatccgtga agcgcatgcc ttccgcggcc atccggtcga tgaacggcgt ctggattttc
     1321 gattcgctgt tgtagcagct gacgtcgccg tagccctggt cgtcggcgaa gatgatgatg
     1381 atgttggggt tggagggcgg tgtcggagcg gcgaaggagg ttgcgcagct gccgcttaca
     1441 gcgagcggag ccagaatatg gagccagttc ggtcgtttca tcggattgat cctttttttc
     1501 aggtgtaggg ccgcaaagca cgaaacatgc gcacattaaa cgcagaactg cggtttgtaa
     1561 tgtactaaaa acagaaaaat cgtctcccgg cgttgccggc tttccggcat gcggccgttg
     1621 tttttcactt tttttctggc gatgaggttt tattctctac ggcctgcatg acccaataac
     1681 catttttaag gaggttccaa tggctgaaaa aacgatggag tttaaaaccg aactgtcgca
     1741 actgctgcat ctgatcaccc attcgctcta ttcgcaccgc gaggtgttcc tgcgcgagct
     1801 catcagcaac gcctgcgacg cgattgataa gatccgcttc gaagcgctga cccgcaccga
     1861 gctgctcgaa ggcggcagcg ataccgactg gaagatcgag ctggccgtcg acaaggaggc
     1921 gaagacgctg acggtttccg acaacggcat cggcatggac gaagaggccc tcgtccgcaa
     1981 cctcggcacc atcgcccatt ccggcaccaa ggcctttctg gcccgcctga aagagtccgg
     2041 cgcgcagaat aatccggagc tgatcggcca gttcggcgtc ggcttctatt cctcgttcat
     2101 ggtcgcggac aaagtgacgg ttgagtcgcg catgcacggc gccccggcgg tccgctgggt
     2161 ttcggaaggc accggctcgt tcaccgtctc cgaaggctcc cgcgaaacgc gcggcacctc
     2221 cgtaacgctg cacctgaaag aggatgcggc ggaatatctg gaagagtacc ggctgcggac
     2281 gctggtgaag aagttttcgg atttcgtcga gcatccggtg atgctgggcg gcgaaacggt
     2341 gaacacgcgc aaggcgatct ggctgcgctc caagagcgag atcacggccg aagagtacaa
     2401 cgagttctat cagcacatct cgcacgactt caacgatccg gccgaagtga tccactacaa
     2461 cgccgaaggg gcgattgagt ttaaggcact gctgttcatt ccggaaaaga agccgttcga
     2521 tatgatgatg ggccacgacc cgaaggcgca cctgaacctg tatgtccagc gcgtcttcat
     2581 cagcaacgag tttgaaaacc tgctgcccgg ctacctccgc ttcgtcaaag gcgtcgtcga
     2641 tgccgccgac ctgccgctga atgtctcgcg cgaaatcctg caggaaaatc cgcagctcga
     2701 caaaatccg
//
LOCUS       DAOPZO010000185         2690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_53379_length_2690_cov_5.078937, whole genome shotgun sequence.
ACCESSION   DAOPZO010000185 DAOPZO010000000
VERSION     DAOPZO010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53379_length_2690_cov_5.078937"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..925)
                     /locus_tag="PKI68_08915"
     CDS             complement(join(166..592,591..925))
                     /locus_tag="PKI68_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611877.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HNX53850.1"
                     /translation="MARRYALRDDQWDRMEKLLPGRADTVGVTAKDNRLFVEAVLYRY
                     RAGIPWRDLPERFGNWSVVHTRFSRWAKSGVWESVFKQLANEADNEYAMIDSTIVRAH
                     QHSAGAQKKRGKAQAIGRSKGGLSTKINATVDALGNPTGFVLTPGQACDLDGADELLP
                     AIAADTVLADKGYDADERVIDLLQAAGKTIVIPPKRNRKNPRSYDKELYKARHLIENF
                     FCRLKQFRAIATRYDKTARNFLSAIYLAASVILLN"
     gene            1115..2632
                     /locus_tag="PKI68_08920"
     CDS             1115..2632
                     /locus_tag="PKI68_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53851.1"
                     /translation="MPYDVFRLGLDGGVLFQHRYFCRGKNAVEPSQHRQREDDFTIFV
                     PFVWPPEQVADSPNKIGHLRMRFHSHTDFLPEFSKDWKLFYRIYPAGGSATFLKVERQ
                     LCPAPVTVDPHTASAHKNAMSRFANREEMKPLLDLCRQGKLFEVQEWIAAGKPVNPPP
                     PVPARQRTQGPLEVAVGLGFNSLVQVLLEGGANTAEPRPGGLGGFCAIEDAVCQRRLD
                     LVELLVKHGADIRDVPMWRVFDTWDTNIMRYFVERGADTKTDNPIAYAFKYKIRTALR
                     IYKEYEERFPDWKRQLNIALRFHTKEQSQKWVSLMLWAGADPFEPGPQILEVEEIDYY
                     DRCAVEIALRHGWTWMMEKIGKMNPTADHAVKFLEAALESRKPETLKLLIEAGFLKRL
                     NTDQKSALIGSLIRFMFSHLLMQEIRHGKNDYSYRSAESSCFSDGMFELLLQNGVKWT
                     PDFAEIKSDRQELVRIGAPGFKTFVRLLKKYHAATEEVLHELTRTGAAQRLLQSK"
BASE COUNT          642 a          686 c          720 g          642 t
ORIGIN      
        1 aagcattaac tgatctggag accgtgctca aactgccgga tccttttggt aatcagtgga
       61 aaaaagcgca agaggcaatc cgccgaatca gcggtataga ctaaggtaca aaaaaaatcc
      121 ccgggaaaac ccggggattt tttcaaagcg agctagggcg tgtcatcaat taagcaaaat
      181 gacggaggct gccaggtaaa tggcggacag gaagttccgg gcggtcttgt catagcgcgt
      241 tgcaatggcc cggaactgtt taagccggca gaaaaagttc tcgatcaggt gtcgcgcctt
      301 gtagagctcc ttgtcatatg aacgagggtt cttccggttt cttttcgggg gaataacgat
      361 tgttttccct gccgcttgta acagatcaat cacccgttca tccgcgtcat atcctttatc
      421 cgccagaacg gtatctgccg cgatggcggg taacagttca tccgccccgt ccagatcaca
      481 ggcctgtccc ggcgtcagaa caaagccggt cggattgccc aatgcatcca ccgttgcgtt
      541 gattttggtg ctcaatccgc ccttgctgcg acctatagcc tgtgctttac cgtttttttt
      601 gagcaccggc actgtgctga tgagcccgga caatcgtact atcaatcatg gcgtattcat
      661 tatccgcttc gttggccaac tgtttaaaaa cactctccca gactccgctc ttcgcccacc
      721 ggctgaagcg tgtatgcacc acgctccaat tcccgaaacg ctccggcaga tcccgccaag
      781 gaatgcccgc acgataccgg taaagcacag cttcaacaaa gagcctgttg tcttttgcgg
      841 ttacgccaac cgtgtctgcc cggcccggca acagcttctc catgcgatcc cattggtcat
      901 ctctcagcgc ataacgtcgt gccatttaaa aaccctccat aaaaagaggg attttagacc
      961 atctttcgtt ataaaacgaa gacttattaa aactaattga tgacacgccc tagctgttga
     1021 ggttcggcgc gagctggttc gggtcatcca gcagccgggc gaagtcgagc ttggacaggt
     1081 tatagaacgg atgctcggtg atctctgaaa gtttgtgccg tatgatgttt tccggcttgg
     1141 ccttgatggc ggcgtgctct ttcaacaccg ctacttttgt cggggcaaga atgcagtcga
     1201 accgtctcag caccgtcagc gggaggatga ctttacgata ttcgttccgt ttgtatggcc
     1261 cccggagcag gttgcagatt ccccaaataa aattggccat ttgagaatgc gtttccatag
     1321 ccataccgac ttccttccag aattttccaa agattggaaa cttttctacc ggatttatcc
     1381 agcgggtgga agcgcgacgt ttttaaaagt cgagagacag ctctgtccgg caccagttac
     1441 agttgaccct cacaccgctt cagcgcataa aaatgccatg agccgtttcg ccaatagaga
     1501 ggagatgaag cctctgctcg atctctgccg gcagggcaag cttttcgagg tgcaggagtg
     1561 gatcgccgcc ggtaagccgg tgaatccccc gccgcccgtt ccggcccgcc agcggacaca
     1621 agggccgctg gaggttgccg tcgggttggg gtttaacagt ctggttcaag tgctgctgga
     1681 gggcggggcc aacacggcag agccaagacc cggcgggctc ggcggatttt gcgcgatcga
     1741 agatgctgtg tgccagagac gtctggattt ggttgagcta ctggtgaagc acggggcgga
     1801 tattcgcgat gttcccatgt ggcgggtgtt cgacacctgg gacacgaata ttatgcgcta
     1861 ttttgtcgag cgtggggccg acacgaagac cgataatccg atcgcgtacg cctttaagta
     1921 taaaatccga accgccctga gaatttataa ggaatacgag gagcggtttc cggattggaa
     1981 gcggcagtta aatatcgcct tgcggtttca cacgaaagag caaagtcaga agtgggtttc
     2041 gctgatgctc tgggccgggg ccgatccgtt tgagccgggg ccgcaaattc tggaggtgga
     2101 ggaaatcgat tactatgacc gatgcgcggt tgaaatcgcc ttgcgtcatg ggtggacgtg
     2161 gatgatggag aagatcggga aaatgaatcc gacggcagat catgcggtca aatttctgga
     2221 ggccgcgctc gaatcacgca aacctgaaac gctgaaactt ctcatcgaag ccgggtttct
     2281 taaacggctg aacacggatc aaaagtccgc gctcattgga agtctcattc gttttatgtt
     2341 cagtcatctg ctgatgcagg agattagaca cggtaaaaac gactactcct accgcagtgc
     2401 agaaagctct tgtttcagtg atggaatgtt cgagctgctt cttcaaaatg gtgtgaaatg
     2461 gacgccggac ttcgctgaaa ttaagtctga tcgtcaggag ttagttcgca tcggcgcacc
     2521 gggattcaaa acctttgtcc ggcttctcaa aaaatatcac gccgccacgg aagaagtcct
     2581 gcatgaactg acccgaaccg gcgccgctca acggctatta cagtccaaat gatgccccgc
     2641 tgaaagtttc tgaatgattt tttaaacttt ttttgaaaaa tcaaacggac
//
LOCUS       DAOPZO010000186         2660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_54361_length_2660_cov_7.471401, whole genome shotgun sequence.
ACCESSION   DAOPZO010000186 DAOPZO010000000
VERSION     DAOPZO010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54361_length_2660_cov_7.471401"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1185
                     /locus_tag="PKI68_08925"
     CDS             169..1185
                     /locus_tag="PKI68_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53852.1"
                     /translation="MNVWTNFWKAGLVFKAAFAEQQTAIKAVYAASDDILHVPAGTPA
                     EYAVSMAPVPDSFCTLRKNIFSTLFQSMYHLMGCDPQHRLFYGKLIHLFRIWVTSADN
                     LLDDEDKVVIPIQMPGSSRVMRQVISIMTADRVFQRLLAEGLASGALTNAQARELSDR
                     SLQILLPSAAQEAGEEAGITERPDPEQVLHVIHRYKTGVLFNVAFLAPEIIEPHPDRA
                     RAAALAEALMQFGIGCQVLDDIRDLARDHIERRHNYLLSVLSKHQPEFFQPLRQRELA
                     VTDRLYLEVPQHTLPAARLGFGMMRDGLATLGELGLGIRPELAGQLAGSMFSVLDLRD
                     VRYA"
     gene            1178..1861
                     /locus_tag="PKI68_08930"
     CDS             1178..1861
                     /locus_tag="PKI68_08930"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNX53853.1"
                     /translation="MHKKQHLRSATAQQTEGVTLDHAAKVYDLLAPVMTLGLERRYHR
                     IVIEQLALRGNEQILDIGCGTGTLTRDIAAALTDKKISRCTGLDAAEKMIGIARRKAL
                     LIPNIRFDAAIAERLPYADSTFDAAVSTFFFHHIHFELKKKVLAETARVLKPGGRFIV
                     ADVDSPTTRFGSVCARSGHWLFQQNEIAENIEGKLRRAFGESPFSWKAVSHHSGYITV
                     FELRKEATP"
     gene            1858..2472
                     /locus_tag="PKI68_08935"
     CDS             1858..2472
                     /locus_tag="PKI68_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53854.1"
                     /translation="MNRFLVLLLMLTALSGRAAQTDDPVGSAVETYFTAVFAKLGEVA
                     AQQPVHDTFRDAMKPAAESVNGFFGGSLITTDFVIDQSYYKRHALAVGFDLKKVKELD
                     YFRNLMRTSPSPQLSEPGHGTILQPRLISMRYPVVVDGKLQSIVSMMVRTETFLEATG
                     LGKCRAYKIICRGALAEEKGILSADHREVKLVLPSTEWVIQYDP"
BASE COUNT          560 a          825 c          709 g          566 t
ORIGIN      
        1 gctcgtcttc atcagcaccc ttgcaggcct cgcgattcaa ctgctatacg gctgagcgcc
       61 ggaggcttcc cgttcaaact gttaatcatc taattaccaa acggtaatcc tgccgcaagc
      121 ttgcggttat ggttatatgt gagtattctt gtcagcgaaa gaaaccacat gaatgtatgg
      181 actaacttct ggaaagccgg cctcgttttc aaagcggcct tcgcagaaca gcaaaccgcc
      241 attaaagcag tttatgctgc gtcagacgat attctgcacg ttccggcagg tacaccggcc
      301 gaatacgctg tctcaatggc ccccgttcct gattccttct gcaccttgcg caaaaatatc
      361 ttttccaccc tcttccagtc catgtaccac ctgatgggtt gcgatccgca gcaccgtctt
      421 ttttacggca aactgattca ccttttccgg atctgggtaa ccagcgccga taacctgctc
      481 gacgatgaag ataaagtcgt catcccgatt caaatgcccg gctcatcgcg cgtcatgcgg
      541 caggtcattt ccatcatgac cgccgaccgc gtcttccagc ggctgctcgc cgaaggttta
      601 gcctccggcg cattgacgaa cgcgcaggcg cgagaactct ctgatcggtc gctgcaaatt
      661 ctcctgccga gcgcggcgca ggaagccggc gaagaggcgg gcatcaccga acgtcctgat
      721 ccggaacagg tcttacacgt cattcaccgc tacaagaccg gtgtgctctt caatgtcgcc
      781 ttcctcgcgc cggaaatcat cgagccgcat ccggatcggg cgcgcgccgc tgcgctcgca
      841 gaagctctga tgcagttcgg catcggctgc caggtactcg acgacatccg cgatctcgcc
      901 cgcgaccata ttgagcgccg ccacaactac ctgctttcag ttctctcgaa acatcagcct
      961 gaatttttcc aacctctgcg gcagcgtgag ctggccgtga cggatcgcct ctatctggaa
     1021 gttccgcagc atacgctgcc cgccgcccgg ctcggcttcg gcatgatgcg cgacggactc
     1081 gccacccttg gagagctggg gcttggcatc cggcctgaac tggccgggca gctggccggc
     1141 tccatgttct ccgtacttga tctgcgggac gtccggtatg cataagaaac agcatctccg
     1201 ctcagctacc gcgcagcaga ccgaaggggt cacgctggat cacgccgcca aggtttacga
     1261 ccttctcgct ccggtcatga cgctcggact tgagcgccgc tatcaccgca tcgtcattga
     1321 gcagctcgcc ctgcgcggca atgagcagat actggatatc ggctgcggaa ccggcacact
     1381 gacacgcgat attgcggccg cgctgacgga taaaaaaata tcgcggtgca ccggactgga
     1441 cgccgcagaa aaaatgatcg ggattgcgcg gcgcaaggct ctgctgattc ccaatatcag
     1501 attcgacgcc gccatcgccg aacggctgcc gtatgccgac agcaccttcg acgccgcagt
     1561 ctccaccttt ttctttcacc acatccattt tgagctgaag aagaaagtgc tggccgaaac
     1621 cgcacgggta ctgaaacccg gcggacggtt catcgttgcc gatgtggaca gtccaaccac
     1681 ccggttcgga tcggtctgcg cacgttccgg ccactggctt ttccagcaga atgaaatcgc
     1741 cgaaaacatc gaaggtaaac tgcgccgggc tttcggcgag agtccgtttt cgtggaaagc
     1801 ggtttcccat cactcaggat acatcaccgt ctttgaactc cggaaagagg cgacgccatg
     1861 aaccgtttcc ttgtcttact tctgatgctg accgcgttat ccggccgggc cgcccaaaca
     1921 gacgatccgg tcggcagcgc agttgaaacc tatttcaccg ctgttttcgc aaagctcggc
     1981 gaagtcgccg cgcagcaacc ggtacacgat acgttccgcg acgccatgaa accggcggcg
     2041 gaatccgtca acggcttttt cggcgggtcg ctgatcacga ctgactttgt catcgaccag
     2101 agctattaca aacgccatgc gctcgccgtc gggttcgatc tgaaaaaagt taaggagctc
     2161 gattatttcc ggaatctgat gcgcacctcg ccgtcaccgc agctcagcga accgggtcac
     2221 ggtacgatcc tgcagccgcg cctgatttca atgcgctatc cggttgtcgt agacggaaaa
     2281 ctccagagca tcgtttccat gatggttcgt acggaaacgt ttctcgaagc aaccgggctt
     2341 ggaaaatgcc gggcgtacaa gatcatctgc cgcggcgcac tggcggaaga aaaaggaatt
     2401 ctttctgccg atcaccgtga agtgaaactc gtcctgcctt cgaccgagtg ggtgattcaa
     2461 tacgatccct agtccccgtc cggagcttcg ctgatgatcg caacaccggc gccggcggag
     2521 aggaagctgc aggcttccgc cgctttatcc gggccggggt tcacacagac cgccgcaacc
     2581 ggtttattga gcagccgggt gcagttcttt gaagttgcct tcgctcaaaa gcgattcctg
     2641 ccaggcggaa gccaggcgct
//
LOCUS       DAOPZO010000187         2613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_55845_length_2613_cov_8.871384, whole genome shotgun sequence.
ACCESSION   DAOPZO010000187 DAOPZO010000000
VERSION     DAOPZO010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55845_length_2613_cov_8.871384"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..1266
                     /locus_tag="PKI68_08940"
     CDS             61..1266
                     /locus_tag="PKI68_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX53855.1"
                     /translation="MIQHLKQRFANAGGDVRRFLAAIAFASFSTSVLDAVFNNFLNET
                     FTISSLTRTLIEIPREMPGLLVMFFSALLFFLCNRRLAAFALTLCSIGLTLLATHSAS
                     LTSVLVWLFVCSTGQHLMIPLQSGIGMELAREGHDGRRLGQINALRNFCAISGAFFVF
                     VGFRYLHLTFTGSFLTASAGFLCGAILLYRMKANRPHPPSMHLKLHKEYRLFYWLSIL
                     FGTRKQIFLTFAPWVLVRVFAQPVQNIAILMTIGGVLGIILQPYLGRLIDTRGERFVL
                     AGEALILIFVCLLYGFSGRLFPGRIALPVIGGCYIADMLLMSVGMARATYLKKIALKP
                     AHIMPALAASTSMDHVFSISAAILCGCIWQKFGYQYVFLIGALVAAINLASALRIRIP
                     AKTAIAFRD"
     gene            complement(1460..2539)
                     /gene="serC"
                     /locus_tag="PKI68_08945"
     CDS             complement(1460..2539)
                     /gene="serC"
                     /locus_tag="PKI68_08945"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNX53856.1"
                     /translation="MARSYNFSAGPSILPEEVLKEAQAELLDYKGTGMSIMEMSHRGK
                     EYSAVHDEAIANLKTLLKIPENYSVLFMTGGATSQFSLIPMNLLGAGQTADYINAGSW
                     GTAAVKAAKKLGKINVIADTSKAIPTCMPENSELKFSDDAAYVHITSNETIAGTQVKE
                     FPKTKAPLVADMSSDILSRPIDVSQFGLIYAGAQKNLAPAGVTLVIIRNDLAERAPET
                     LPELFQYRAFIEENSLKNTPPTFSIYILALVTRWLIAKGGLEGMQKINEAKAAKLYAA
                     IDASSFYKGTALPKWRSPMNVTFRLPTEELEEQFIKAASKLGMTSLKGHRSVGGIRAS
                     IYNAFPEAGVDALVAFMKDFEAKNS"
BASE COUNT          494 a          757 c          718 g          644 t
ORIGIN      
        1 gcctccgaag ccggtgatct gggttcgact cccggcaggt gcaccagcct tcgctccctt
       61 atgatacaac atctcaaaca gcgttttgca aacgccggcg gcgatgtccg ccgctttctg
      121 gcggccatcg ccttcgccag tttttccacc agcgttcttg atgcggtctt taataatttc
      181 ctgaatgaaa ccttcaccat cagcagtctg acccggacac tgatcgaaat accgcgcgaa
      241 atgccgggcc tgctggttat gttcttttcc gctctgctct tcttcctgtg caaccggcgg
      301 ctggccgcct tcgcgctgac actgtgctcg atcggactga ccctgctcgc aacgcattcg
      361 gcctcgctga cgtctgttct ggtctggctc ttcgtctgca gcaccggtca gcatctgatg
      421 attcctcttc agtccgggat cggcatggaa ctggcccgcg aaggacacga tggacgaagg
      481 ctcggccaga ttaatgcgct ccgaaacttt tgtgcgatca gcggagcctt tttcgtcttc
      541 gtcgggttcc gctatctgca cctcactttt acgggctctt tcctgaccgc ttcggccgga
      601 ttcctttgcg gcgcgattct gctttaccgg atgaaagcca accggccgca tccgccctcc
      661 atgcacctga agcttcacaa agaataccgg ctgttttact ggctctcgat cctcttcggc
      721 acccgcaaac agatcttcct caccttcgcg ccgtgggtgc tggtccgggt ttttgcgcag
      781 cccgtgcaga atatcgccat cctgatgacc atcggcggag ttcttggaat catcctccag
      841 ccgtatttag gacgattgat tgataccagg ggcgaacggt ttgtactggc gggcgaagct
      901 ctgatactga ttttcgtatg tctgctctac ggtttttcgg gccgcctgtt tcccggccgc
      961 atcgcccttc cggtcattgg cggatgctat atcgccgaca tgctcctgat gtcggtcgga
     1021 atggcgcgcg ccacctacct gaaaaaaatc gcgctgaaac cggcgcacat catgcctgcg
     1081 ctggccgcct cgaccagcat ggatcacgta ttctccattt cggccgccat tttatgcgga
     1141 tgcatctggc agaaattcgg ctaccaatac gtcttcctga tcggcgcctt ggtggccgcc
     1201 atcaatctgg cgtccgcgct ccggatccgc attcctgcca aaactgctat cgccttccgc
     1261 gattaagatg ttcggtatag ccggaactca ggcgaagccg tgggtccggc tattaacact
     1321 cagaagccgg atctacgggc gaataccccc taagttccgg ctttaccgtt tgcgaagatt
     1381 aaatcgttgc gtgctctaaa taaaaatccg cagccacgcg aacgtgattg cggattgccg
     1441 gtcggaccga agccgggctc tagctgttct tggcttcaaa gtccttcatg aaggcgacga
     1501 gtgcatcgac tccggcttcc ggaaaagcgt tatagatgga agcgcggatt ccgccaaccg
     1561 aacggtgtcc cttcaggctg gtcatgccca gcttggaagc ggctttgatg aactgctctt
     1621 ccagctcttc agtcggcagg cggaaagtca cattcatcgg cgaacgccat ttcggaagcg
     1681 ccgtgccttt gtagaagctc gatgcgtcaa tcgcggcgta cagcttggcg gctttggctt
     1741 cgttgatttt ctgcatccct tcgaggccgc ccttggcgat cagccagcgg gtaaccagcg
     1801 cgaggatata gatcgaaaag gtcggcggcg tgttcttgag cgaattctct tcgatgaaag
     1861 cgcggtactg gaacagctcc ggaagcgttt caggtgcacg ttcggccagg tcgttgcgga
     1921 tgatgaccag cgtcacgccc gccggagcga gattcttctg cgcgccggca taaatgagcc
     1981 cgaactgcga aacgtcaatc ggacgcgaaa gaatgtcgga cgacatatcg gccaccagcg
     2041 gagctttggt tttcggaaat tccttaacct gagttccggc gatggtttcg ttggaagtga
     2101 tgtgaacgta agcggcgtca tcgctgaact tcagctcgct gttttccggc atgcaggtcg
     2161 gaattgcttt ggaggtatcg gcgatcacat tgattttgcc gagctttttg gcggctttga
     2221 cggccgcagt gccccacgaa ccggcgttga tgtaatcagc ggtctgccct gcgccgagca
     2281 ggttcatcgg gatcagcgaa aactggctgg tcgcgccgcc ggtcatgaaa aggacgctgt
     2341 agttttccgg aattttcagc agggtcttga ggttggcgat cgcttcgtca tgcacggcgg
     2401 aatattcctt gccgcggtgg ctcatctcca tgatggacat gccggttcct ttgtagtcca
     2461 gcagctcagc ctgagcttct ttaaggactt cttccggtag aatcgacgga cctgcactga
     2521 aattatatga acgagccatg atgacctttc ctttcggttg aaaattgcgg ccaacatcta
     2581 gcacggcgag ccctgcgcgg gcaagtgagg aat
//
LOCUS       DAOPZO010000188         2572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_57208_length_2572_cov_10.756456, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000188 DAOPZO010000000
VERSION     DAOPZO010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57208_length_2572_cov_10.756456"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1414)
                     /gene="hcp"
                     /locus_tag="PKI68_08950"
                     /gene_synonym="priS"
     CDS             complement(<1..1414)
                     /gene="hcp"
                     /locus_tag="PKI68_08950"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNX53857.1"
                     /translation="MSMFCYQCEQTAKCTGCTSFGVCGKDPDTATLQDLLVEVCKQIA
                     VAAKGKQNRAADLFVMEGLFTTVTNVNFDAEDIAGVIRRGGEVLRELGAKSTVNFAAD
                     LSGLIRQGEAVGIESRRKALGEDITGLQELILYGLKGTAAYADHAYILGKEDPAVFAF
                     FHEALAVLKNGPATVDVLLPLALKVGEVNLTVMGLLDAANTGTYGHPVPTPVRITPVQ
                     GKAILVSGHDLKDLELLLKQTEGKGINIYTHGEMLPCHGYPELKKYKHLAGNYGGAWQ
                     DQREEFEAFPGAILMTTNCIQKPKENYKARIFTCGLVQWPGVQHIADGNFAPVIQAAL
                     EAPGFAATEPEKTILVGFGHNAVLGVAPTVIEAVKSGALKHFFLIGGCDGAKPGRNYY
                     TEFAEAVPEDCVILTLACGKYRFNKLEFGAIGGIPRLLDVGQCNDAYSAIQIAVALAG
                     AFGCGVNDLPLSMILSWYEQK"
     gene            1521..2549
                     /locus_tag="PKI68_08955"
     CDS             1521..2549
                     /locus_tag="PKI68_08955"
                     /inference="COORDINATES: protein motif:HMM:NF033936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SO_0444 family Cu/Zn efflux transporter"
                     /protein_id="HNX53858.1"
                     /translation="METAVSIIVKSWDVMAIMAPYLLLGFFVAGLLGAFVPVSFIETH
                     LGKRGIWQIVKASLLGVPIPLCSCSVIPVAASLRRHGATKGATISFLTSTPQTGVDSI
                     AATWGLLGPLFAVFRMAVAFITGIICGAAVEALTPSSEESAGACDDEHCPSCKPESGA
                     GKWKQVFAYGFGSLPREIGRALLIGILISGLLGALVPDDFFTRYLNSEWLSMLAVMAL
                     GIPLYVCSTGSIPVALAMIGMGLSPGAALAFLITGPATNAATIATVFQTMGRRAVIIY
                     LATLAGCSLAAGWLLNRIISAQGITEQVHHHAAEAGRFEYLCALILAGLLVSGVLPRK
                     KKSCCHAG"
BASE COUNT          441 a          773 c          775 g          583 t
ORIGIN      
        1 ctttctgttc gtaccacgac aggatcatcg agagcggcag gtcgttgacg ccgcagccga
       61 acgcgccggc cagtgcgacg gcgatctgga tcgccgagta ggcgtcgttg cactgcccga
      121 cgtcgagcag gcgcgggatg ccgccgatgg cgccgaactc cagtttgttg aagcggtatt
      181 tgccgcaggc cagcgtgagg atcacgcagt cttccggtac ggcttccgca aactcggtgt
      241 agtagttgcg gcccggcttc gcgccgtcgc agccgccgat gagaaagaag tgcttcagcg
      301 caccgctctt gacggcttcg atgacggtcg gcgccacgcc gaggacggcg ttatggccga
      361 atccgaccag gatggttttt tccggttcgg tcgcggcaaa gccgggggct tcgagcgcgg
      421 cctggatgac cggcgcgaaa ttgccgtcgg cgatgtgctg cacgccgggc cactggacga
      481 ggccgcaggt gaagatgcgg gctttgtagt tttccttcgg cttctggatg cagttggtcg
      541 tcatgagaat cgcgccgggg aaggcttcga actcttcgcg ctggtcctgc cacgcgccgc
      601 cgtagttgcc ggcgaggtgt ttgtatttct tcagctccgg atagccgtgg cacggcagca
      661 tttcgccgtg ggtgtagatg ttgatgcctt tgccttcggt ctgcttgagg agcagttcga
      721 ggtctttcag atcgtggccc gaaacgagaa tggctttgcc ctggacgggc gtgatgcgca
      781 ccggcgtcgg aaccggatgg ccgtaggtgc cggtattcgc ggcgtcgagc aggcccatga
      841 cggtcaggtt gacttcgccg actttcaggg ccagcggcag caatacgtcc acggtggccg
      901 gaccgttttt gagaaccgcc agcgcttcgt ggaagaaggc gaaaaccgcc gggtcttctt
      961 tgccgaggat ataggcgtgg tcggcatagg cggccgtgcc tttcagtccg tagagaatca
     1021 gctcctgcag gccggtaatg tcttcgccga gcgcttttct gcggctttcg ataccgaccg
     1081 cttcgccctg ccgaatcagt ccgctgagat cggcggcaaa gttgacagtc gatttcgcgc
     1141 cgagctctct gagtacttcg ccgccgcggc ggatgacgcc cgcgatatct tcggcgtcaa
     1201 agttgacgtt ggtgacggtg gtgaagagcc cttccatcac aaacagatcg gcggcgcggt
     1261 tctgtttacc ttttgcggcg acggcaatct gtttgcacac ctcgaccagc agatcctgca
     1321 gcgtggcggt atccggatct tttccgcaca ctccgaagct ggtgcatccg gtgcatttgg
     1381 cagtctgttc acactgatag caaaacatac tcataatatt cactcctgtt tttgggtttc
     1441 tgtaacctat gccacggact atacttgctt ccgaaaagag aaccttgacc tgcgtcaagt
     1501 ttgtgaaaaa aggtgccgtt atggaaactg ccgtcagcat aatcgtcaaa tcgtgggatg
     1561 tcatggcaat tatggctccc tatcttctgc tcggtttttt cgtagcgggt ctgctggggg
     1621 ctttcgttcc ggtttctttc attgaaacac acctcggtaa acgcggcatc tggcagattg
     1681 taaaagcctc gctgctcgga gtgccgattc cgctctgctc gtgcagcgtc attccggttg
     1741 ccgcctcgct gcgccgccac ggcgcaacaa agggcgcaac catttctttt ctcacctcca
     1801 ccccgcagac cggtgtagac agtattgccg ccacgtgggg actgctcggt ccgctcttcg
     1861 ccgtcttccg gatggccgtg gcgttcatca ccggaatcat ctgcggcgcc gcggtcgaag
     1921 cgcttacccc ttcatccgaa gaatccgcag gtgcatgcga cgatgaacac tgcccttcct
     1981 gcaaaccgga aagcggtgcc ggcaaatgga agcaggtttt cgcctacggc ttcggctcgc
     2041 ttccgcggga aatcgggcgc gcactgctga tcggcatcct gatttccggg ctgctcggcg
     2101 cgctggtacc cgacgacttc ttcacccgct acctgaactc cgagtggctc tccatgctgg
     2161 ccgttatggc gctcggtatc ccgctgtatg tctgctcaac cggatccatt ccggtcgcac
     2221 tggcaatgat cggcatggga ctttcgccgg gggcggcgct cgcattcctg atcaccggcc
     2281 cggccactaa tgcagccacc attgccaccg tgtttcaaac aatggggcgc cgcgctgtga
     2341 ttatttatct ggctacactc gcgggctgtt cgctggctgc cggctggctg ctcaaccgga
     2401 tcatcagcgc acaggggatc actgaacagg tgcaccatca cgccgccgaa gcggggcgct
     2461 ttgaatacct ctgcgctctc atcctcgccg gactgctggt ttccggcgtt cttccgcgca
     2521 aaaaaaagag ctgctgccac gccggataac ctcattcttt cagcgcggcc cc
//
LOCUS       DAOPZO010000189         2560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_57651_length_2560_cov_9.500200, whole genome shotgun sequence.
ACCESSION   DAOPZO010000189 DAOPZO010000000
VERSION     DAOPZO010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57651_length_2560_cov_9.500200"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PKI68_08960"
     CDS             <1..475
                     /locus_tag="PKI68_08960"
                     /inference="COORDINATES: protein motif:HMM:NF024374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HNX53859.1"
                     /translation="YANAVAWGTTPEEKKQNAYNASLLPTWWGHDEGQPHTLFDYGWR
                     EWNGLVGGYYGGRWELFLNYLEECLKNGVAYDDTKDLNSWGRHSMDGNEIFKKMMAFE
                     ESWIAKEKNYPATPVGDSIAVARELLAKYRTVLQSTKSEVDPEWNPKQKVQENMQ"
     gene            543..>2560
                     /locus_tag="PKI68_08965"
     CDS             543..>2560
                     /locus_tag="PKI68_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase family 8 super-sandwich
                     domain-containing protein"
                     /protein_id="HNX53860.1"
                     /translation="MNFGRWITAGLLAVGLLEAGAAETLLVNYFDIPVASPAGTEVCG
                     RIHLDSNRDAHHHRIPETYHFEILSDPSGLFELDAQRDSESRLFGALRIASGHELPKT
                     PTNFVLQVALRDGSTLLASTNLTVYAVAQTTLKRALTYLDRLTQDESRFYGRRRYTDA
                     QVAALITEVEQRQGGFASLGFYTRDLLSYRKSGGQLGREWEEASNIIGGLGYAYRTSK
                     QYGPAGDPAAHQRLKRALYLAISRYAELVPMAGEDVVIDGKPISSNYGDGFNLLEGSL
                     GMVEHNYISHPWRFHDALSGPAVWLMPELLKDARAGDPLAIRTHEKLIRIFQLAFSNP
                     PSYQASDNEDGRWGDLTDPHHTEGAYSDANLGHRLRSWMTLLGIWADYNRPITYIPNW
                     YPGFYTGPTGENLQFMPGWTPHGVLPDLTFWVTHFHRPAHYFVQSGFQPDGTVSHHLP
                     KSSDIAMHAYGYGWLTEVLDGYLLLKDEPVDLGSHGYQFIADRLLYSYDKMIYNGAMD
                     FTVCGRSYFGNMKKFVDGVAHDVTRMMEAKQAGTVITNEAALLAWRDALLNRTQVCSG
                     NFPFWVGQFMVHRRGGNGEAPCYYSVKMENDRTNGAEDFEKVGKSYHSGSGLLQAKVR
                     GDEYEDTRYNWDWHALPGLTEEWRTDPIPVKPGIAFGGSPFAAMASDGR"
BASE COUNT          507 a          773 c          786 g          494 t
ORIGIN      
        1 gtatgcgaat gccgtggcct ggggaacgac gccggaagag aagaaacaga acgcctacaa
       61 cgcttcgctg ctgccgacct ggtggggtca tgatgaaggt cagccgcata cgctgttcga
      121 ctacggctgg cgcgagtgga acggcttggt tggcggctac tacggcggcc gctgggaact
      181 tttcctcaac tatctcgagg agtgtctgaa gaacggcgtg gcctatgacg atacaaaaga
      241 tctcaattcc tggggccggc actcgatgga cggcaacgaa atcttcaaga agatgatggc
      301 cttcgaagag tcgtggatcg ccaaagagaa gaactatccg gcgaccccgg tcggcgacag
      361 catcgcggtt gcgcgggagc tgctggccaa ataccgcacg gttctgcagt cgacgaagag
      421 cgaagtcgac cccgagtgga atccgaaaca aaaagtacaa gaaaacatgc agtaatccgt
      481 ttgatgtacg ttcagggcct gccggcagtg tgccggcagg ctgactgtat caggaggtcg
      541 ctatgaattt cggacgatgg attacagccg ggcttctcgc cgtcggactg ctggaagcgg
      601 gtgccgcaga aaccttgctg gtgaattatt tcgatattcc ggtcgccagt ccggccggaa
      661 ccgaagtgtg cggccggatt catctcgact ccaaccgcga cgcccatcat caccgcattc
      721 ctgaaaccta tcattttgaa atcctttccg atccgtcggg gctctttgag ctcgacgccc
      781 agcgcgattc ggaaagccgt ctgttcggcg ccttgcggat tgcctccggg catgagctgc
      841 cgaagacccc cacgaatttt gttttacagg tcgcgctgcg cgacggctcg acgcttctgg
      901 cttccaccaa tctcacggtg tatgcggttg cgcagaccac cctgaagcgg gcgctgacct
      961 atctggaccg tctgacgcag gacgaatccc gtttctacgg ccgccgccgg tataccgacg
     1021 cgcaggtcgc ggcgctgatt accgaagtgg agcagcgcca gggcggtttt gcgtcgctcg
     1081 gattttatac ccgcgatctg ctgagttacc gcaagagcgg cgggcagctg ggccgcgaat
     1141 gggaagaagc ctcgaacatc atcggcggtc tgggctacgc ctaccgcacg tcgaagcagt
     1201 acggccccgc cggcgatccg gcggcgcacc agcggctgaa acgggcgctg tatctggcga
     1261 tcagccgcta tgccgagctc gtgccgatgg ccggcgaaga cgtggtcatc gacggcaagc
     1321 cgatttcttc gaattatggc gacggcttta acctcctcga ggggtcgctg ggcatggtgg
     1381 aacataacta catctcgcat ccgtggcgct ttcacgacgc gctttcgggc ccggccgtct
     1441 ggctgatgcc ggagctgctg aaggatgccc gcgccggcga tccgctggcg attcggaccc
     1501 acgaaaaact catccggatt ttccagctgg ccttcagcaa cccgccgagc tatcaggcct
     1561 ccgataatga agacggccgc tggggcgatt tgacggatcc ccatcacacc gaaggcgctt
     1621 attcggatgc caacctcggc caccgcctgc gcagctggat gacgctgctc gggatctggg
     1681 ccgactacaa ccggccgatc acctacattc cgaactggta tccgggattt tataccggcc
     1741 cgacgggcga aaaccttcag ttcatgccgg gctggacccc gcacggcgtc ctgccggacc
     1801 tgactttctg ggtgacgcat ttccaccggc ccgcgcatta ctttgtccag tcgggcttcc
     1861 agccggacgg cacggtgtcg catcacctgc cgaagtcctc cgacatcgcc atgcatgcct
     1921 acggctatgg ctggctgacc gaggttttgg acggctattt gctgcttaaa gacgagccgg
     1981 tcgatctggg cagtcacggc tatcagttca tcgccgaccg gctgctctac agctacgaca
     2041 agatgatcta taacggcgcc atggacttca cggtctgcgg ccgcagttat ttcggcaaca
     2101 tgaaaaagtt tgtggacggg gtggcccacg acgtgacgcg gatgatggag gccaagcagg
     2161 ccggcacggt gattaccaac gaggccgcac tgctcgcctg gcgcgacgcg ctgctcaacc
     2221 gcacgcaggt ttgctccggc aactttccgt tctgggtcgg ccagttcatg gttcatcggc
     2281 gcggcggcaa cggcgaagcc ccgtgctatt actccgttaa aatggagaac gaccggacca
     2341 acggcgccga agattttgag aaagtcggca agtcctatca ttccggcagc ggtctgctgc
     2401 aggccaaggt gcgcggcgat gagtatgaag acacccgcta taactgggac tggcacgctc
     2461 tgccggggct gaccgaagag tggcgcaccg acccgattcc cgtcaaaccg gggatcgctt
     2521 tcggcggcag ccccttcgcg gcgatggcct ccgacggccg
//
LOCUS       DAOPZO010000190         2518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_59139_length_2518_cov_6.678035, whole genome shotgun sequence.
ACCESSION   DAOPZO010000190 DAOPZO010000000
VERSION     DAOPZO010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59139_length_2518_cov_6.678035"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1141
                     /locus_tag="PKI68_08970"
     CDS             119..1141
                     /locus_tag="PKI68_08970"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNX53861.1"
                     /translation="MKLAPLKFGSVTIDFPVVLAPMAGYTDAAFRSICKENGAGACYT
                     EVTSAEGIRRDSQKTFQFLEVSKLDHPIAGHIFGKSVEAMVDAAVYIEKLGTFDWIDL
                     NCGCPVKKVVRRGAGAALMKEPEQIGEIVRAVKKAVSLPVSVKTRIGFSPAFPDHLTI
                     AKIVEDAGADMIAVHGRYATNFHGGPADWEKLGQIKQALKIPVIGNGGVTTLAEADEM
                     VRVSGVDGVMIGRGAVGNPWLFGQIAQAWRGETPVLPTLAQRRQVIETHLLRLAEMTT
                     ATAQGRRCKTKYIPEQAACLHFRPHLAQYIKGLRGRRDLLQHLNEMHDVNTVLAAVDK
                     VLEQNL"
     gene            complement(1599..2312)
                     /locus_tag="PKI68_08975"
     CDS             complement(1599..2312)
                     /locus_tag="PKI68_08975"
                     /inference="COORDINATES: protein motif:HMM:NF022354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection system antitoxin AbiGi family
                     protein"
                     /protein_id="HNX53862.1"
                     /translation="MKPKSDNLFHFTKTLDVLKSILKNGVYPKYCLEDFEWADMDIRS
                     IAYPMCCFCDIPLSRIEEHTDFYGNYGIGLSKDWALRNKLEPVIYATQNGSVQKLTKY
                     LFGAGEKNENPSKELSDVFYRCVSMVKPITGKIVIKRSILEKDFYQENEWRFVPPVDT
                     ILFEEELENQKDSANQAIEKYKLEIAPNDIRYIFVKSDNDIPSLADFITTDMGNYPHN
                     DLKILQTRIVSLTTLKADL"
BASE COUNT          564 a          624 c          690 g          640 t
ORIGIN      
        1 cgtccgctcc tccaacaccg agccgctcgt ccgcctcaac ctcgaagcca aaaccgcggc
       61 tcagatggaa cagaagcgcg acgaagtgct ggcgctgatt cggggctagg ctcatttaat
      121 gaaacttgct cctttaaaat tcggcagcgt caccatcgat tttccggtgg tgctcgcgcc
      181 gatggccggc tacaccgacg cggcgttccg ttcaatctgc aaagaaaacg gcgcgggcgc
      241 ctgctatacc gaggtgacca gcgccgaagg catccgccgc gattcgcaga agacctttca
      301 gtttctggaa gtgtccaagc tcgaccaccc gattgccggc catattttcg gcaagtcggt
      361 cgaagcgatg gtggatgcgg cggtttatat cgaaaagctc ggcacgttcg actggatcga
      421 cctcaactgc ggctgtccgg tcaaaaaagt cgtccggcgc ggcgcgggcg ccgcgctgat
      481 gaaggagccg gagcagatcg gcgagatcgt ccgcgccgtc aagaaagcgg tttcgctgcc
      541 ggtgagcgtg aagacccgca tcgggttcag cccggcgttt ccggatcatc tgacgattgc
      601 caaaatcgtc gaggatgccg gcgcggatat gatcgcggtg catgggcgtt atgccactaa
      661 ttttcacggc ggcccggcgg actgggaaaa gctcggccag atcaaacagg cgctgaagat
      721 tccggtgatc ggcaacggcg gcgtgacgac actggcggag gccgatgaaa tggtgcgggt
      781 ttccggcgtc gacggcgtga tgatcggccg cggcgcggtc ggcaatccgt ggctgttcgg
      841 ccagattgcg caggcgtggc gcggcgaaac gccggttctg ccgacgctgg cgcagcggcg
      901 gcaggtgatc gaaacgcatc ttctgcggct ggccgagatg accactgcga cggcacaggg
      961 gcggcgctgc aaaaccaaat acattccgga acaggcggcc tgcctgcatt tccggccgca
     1021 tcttgcgcaa tacattaaag gcctgcgcgg gcgccgcgat cttttgcagc atttgaacga
     1081 aatgcatgat gtaaacaccg tgctggcggc ggtcgataaa gtactggagc agaatttata
     1141 aatgtggagt gcgcaggctt gactgcgctt tacggagcag atcggcgccc ggcattccgg
     1201 cctgagcctc gttcgagatg ccgataatct gttcttcggt gtgacgcttc ttcatccctg
     1261 agtcctcctt ctcggtttta ccacggaaaa ctctcagctc aaaccgtcca gtttcacggg
     1321 ctcaggtcac tttttcaaac aaacaaagct cggatcggaa tttctccatt ccttttagga
     1381 tttccttcat agtgttagtg attggatagt cgttttgtcg ttgggttata gcggttcaaa
     1441 gccgccgatg ctctgtaaaa ctggtgcata ggttaagctg ttccaacctt tggaaaattt
     1501 caccgcgaaa gacgtgcggg gaatctaagc gaggttgcat aacggattct gagggagacc
     1561 caggcaagat cacagataag gctgagcgat aacatgcttc ataagtcggc ctttaatgtg
     1621 gttagcgaca caattctggt ttgcagtatt tttaaatcat tgtgagggta attacccata
     1681 tcggtggtga tgaagtcagc tagagaggga atatcgttgt cgctttttac gaaaatataa
     1741 cgaatatcgt taggcgctat ttctagtttg tatttttcaa tcgcttggtt ggctgaatct
     1801 ttttgattct caagctcttc ttcgaagagg attgtatcta cgggtggaac aaagcgccac
     1861 tcgttttctt gatagaagtc tttttcgaga atcgatcgct ttataactat ctttcccgtg
     1921 atgggtttaa ccatagaaac acagcggtag aaaacgtcgc ttagttcttt gcttgggttt
     1981 tcattttttt cccctgcccc gaatagatat ttggtcagtt tctggacaga tccgttttgt
     2041 gtggcgtaaa taaccggctc caatttattt cgtagtgccc agtctttgct taaccctatt
     2101 ccgtaatttc catagaagtc cgtgtgttcc tcaatcctgc ttaacgggat gtcgcaaaag
     2161 caacacatcg gatacgcaat gcttcttata tccatgtccg cccattcgaa atcttcgaga
     2221 caatattttg gataaactcc gtttttcagg atgcttttga gcacatcaag agtttttgta
     2281 aaatggaaaa ggttatcaga ttttggtttc atagcgcatg ttgcggttaa ggtgtattcg
     2341 tcattctatt gcaccccaaa gacaaagagc ttatagcagg catgagagga gcgttcaatc
     2401 ttccaaagga tgggaaaaat gggtctgtgc gccgcaaagc gctgtgccgc aacgcagttg
     2461 ctggaaaatc gggtttggca tcccttccca ttcgtgtaca ttcgcgtcca ttcgtggt
//
LOCUS       DAOPZO010000191         2515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_59248_length_2515_cov_10.868293, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000191 DAOPZO010000000
VERSION     DAOPZO010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59248_length_2515_cov_10.868293"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /gene="glyA"
                     /locus_tag="PKI68_08980"
     CDS             <1..1214
                     /gene="glyA"
                     /locus_tag="PKI68_08980"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNX53863.1"
                     /translation="DPEMYKILRDENVRQKNNIELIASENIVSQAVREAQGSRLTNKY
                     AEGYPGKRWYNGCECADEAERLAIDRAKQMFGAEHVNVQPHCGSSANMAVYYAVLEPG
                     DTILAMSLAEGGHLTHGHPMNFSGRFFKIVPYGVRKDTEQIDYDELQRLAEEHKPRML
                     VAGASAYSRIIDFKRMRKIADSVGAYFMVDMAHIAGLVAAGCHPSPVPDADFVTTTTH
                     KTLRGPRGGMILCKEKFAADIDRQVFPGIQGGPLMHVIAAKAVAFGEALLPEFKTYQQ
                     QVVKNAKALAEAMQANGFRIVSGGTDNHLMLVDVSSAGLTGKDVANALDKASITANKN
                     GIPFDQKSPFVTSGVRLGTPAVTTRGMKEEDMVQIANFFKAVTDNMADEKKLAEIRAE
                     VIAFSNRFPLL"
     gene            complement(1217..1642)
                     /locus_tag="PKI68_08985"
     CDS             complement(1217..1642)
                     /locus_tag="PKI68_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53864.1"
                     /translation="MSKNKNTAGPELDIAGLLALKTYERPDKARAEKNIQNIMRAVRS
                     ADRTPSLLIFPDKSAAWMFAQPRYGVAALFILFLGLHMLSRPMPAASLGTANLEKPEL
                     ASTVAAARTNQLNTAAVPGLTPTYTSLAGEPAPLSSYVK"
     gene            complement(1639..2244)
                     /locus_tag="PKI68_08990"
     CDS             complement(1639..2244)
                     /locus_tag="PKI68_08990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX53865.1"
                     /translation="MTKNPKNEPSSEIPDDLDLVRRSQAGDTAAYEELIRRYHGKIYG
                     LVYSMTSSREDAEDLAQEVFVKAWKALGHFREQSGFYTWIYRIALNRTINFRKRRNRR
                     QTVDFDEFDPDIKTAESYREFSSKGSVLRKMSLGEFQEKMNAALLKLSEKHRAVVVMH
                     DVQEMPHADIAKVMHCSEGTVRSRLFYAHKQLQAELAEFMK"
BASE COUNT          560 a          713 c          704 g          538 t
ORIGIN      
        1 ccgatccgga aatgtacaaa atcctgcgcg acgaaaacgt ccggcagaaa aacaatatcg
       61 aactgatcgc ttcggagaat atcgtcagcc aggccgtgcg cgaagcgcag ggttcgcgcc
      121 tgaccaacaa atacgctgaa ggctacccgg gcaagcgctg gtacaacggc tgtgagtgcg
      181 ccgacgaagc cgaacggctc gccatcgacc gcgccaaaca aatgttcggc gccgaacacg
      241 tcaacgtcca gccgcattgc ggcagcagcg ccaacatggc ggtttactat gccgttctgg
      301 aaccgggcga caccattctc gccatgagtc tcgccgaagg cggacacctg acgcacggcc
      361 atccgatgaa tttttcaggc cgcttcttca agatcgttcc ttacggcgtc cgcaaagaca
      421 ccgaacagat tgattacgac gaactgcagc gcctcgccga agaacacaag ccgcgcatgc
      481 tggtcgccgg agccagcgcc tattcgcgca ttatcgattt taaacggatg cgcaaaatcg
      541 ctgactccgt cggcgcctac ttcatggtcg atatggcgca catcgccgga ctcgtggcgg
      601 cgggctgcca cccgagcccc gtgccggacg ccgatttcgt gaccaccacg acgcacaaaa
      661 cgctgcgcgg tccgcgcggc ggcatgatcc tctgcaaaga aaaattcgcc gcagatatcg
      721 accggcaggt tttccccggc attcagggcg gcccgctgat gcacgtcatt gccgccaaag
      781 cggttgcgtt tggcgaagcg ctgctgccgg aatttaaaac ctaccagcag caggttgtta
      841 aaaacgccaa ggcgctggcg gaagccatgc aggccaatgg attccgaatt gtttccggcg
      901 gaaccgacaa ccatctgatg ctggtggacg tcagttcggc gggactcacc ggtaaagatg
      961 tggccaatgc tctggataaa gcttcgatca ccgcgaacaa aaacggtatt ccgttcgacc
     1021 agaagagccc gttcgttact tccggtgtgc gtctcggcac gcccgcagtc accactcgcg
     1081 gcatgaagga agaagatatg gtgcagatcg caaacttctt caaggctgtt acggataaca
     1141 tggcggacga gaaaaagctg gcggaaatcc gcgctgaagt aatcgccttt tccaaccgtt
     1201 ttccgctgct gtaagcttac ttgacgtaag aagaaagcgg ggcgggttcg ccggccagcg
     1261 acgtataggt cggcgtaagc cccgggactg cagcagtgtt cagctggttc gttctcgcgg
     1321 cggcaacagt cgatgccagt tccggttttt ccagattcgc tgtcccgagt gatgccgcgg
     1381 gcataggccg gctgagcatg tgaagcccga gaaaaaggat aaacagcgct gcaacgccgt
     1441 atcgcggctg ggcaaacatc catgcagcac tcttgtcggg aaaaatcagc aaggacggcg
     1501 ttctgtcggc cgagcggact gcgcgcatga tgttctggat gtttttttca gcgcgggctt
     1561 tatccggacg ttcataggtt ttgagcgcca gcaaaccggc gatgtccagc tcagggcctg
     1621 ctgtattttt gtttttgctc atttcataaa ctccgccagt tccgcctgta actgtttgtg
     1681 cgcataaaag agccgcgacc ggacagtacc ctcagaacag tgcatcactt tggcgatatc
     1741 tgcatgaggc atttcctgca catcgtgcat tacaaccacc gctctgtgtt tttccgacag
     1801 cttaaggagg gcggcgttca ttttttcctg aaactctccc agactcattt tgcgcagaac
     1861 cgatcctttg ctggaaaatt cccggtagga ttcggcggtt ttaatgtccg gatcaaactc
     1921 gtcgaagtca acggtctgcc ggcggttgcg ccgtttacgg aagttaatgg tgcgattcag
     1981 ggcaatccgg tagatccagg tgtaaaatcc ggactgttcc cggaaatggc cgagcgcctt
     2041 ccaggcttta acaaaaacct cctgcgccag gtcttcagca tcttcccggc tgctggtcat
     2101 gctgtaaacg agtccgtaaa ttttaccgtg gtagcggcgg atcagttctt cgtaggcggc
     2161 ggtatcaccg gcctgtgaac ggcgaaccag atcaagatcg tccgggattt cggaactcgg
     2221 ctcgtttttg gggttttttg tcatgtatac caattatccg ggaaagcgga ctttctccag
     2281 agactgcgac tgcttctacc agttttctca acccttggaa aaagaaaagc gccccgaaaa
     2341 tttccggggc gctttttgaa ccgttttacc gggttcggac ttaaccctga atggctgcct
     2401 gggcggcagc cagccgggcg atcggcacgc ggaacggcga gcagctgacg tagttaaggc
     2461 ctgctttaca gcagaacttc acgctggccg ggtcaccgcc gtgttcaccg cagat
//
LOCUS       DAOPZO010000192         2458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_61343_length_2458_cov_8.706201, whole genome shotgun sequence.
ACCESSION   DAOPZO010000192 DAOPZO010000000
VERSION     DAOPZO010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61343_length_2458_cov_8.706201"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PKI68_08995"
     CDS             complement(<1..353)
                     /locus_tag="PKI68_08995"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HNX53866.1"
                     /translation="MKKLFMVLVLICTAAATAPAQSAYDIKLMTPAVKSALDGRKSRF
                     AELKTLKTRGVVGETNRGYVQALGGGADVQALVAAENKDRKLVYEAIVEQNKLGAAAL
                     ATVETVFARVQRDKA"
     gene            complement(369..539)
                     /locus_tag="PKI68_09000"
     CDS             complement(369..539)
                     /locus_tag="PKI68_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53867.1"
                     /translation="MKYLFLAAALMCCAGCTVKAVGDPNRPITINAHVTIDIKGLEKT
                     ASGIEEYVSSGK"
     gene            complement(557..1570)
                     /locus_tag="PKI68_09005"
     CDS             complement(557..1570)
                     /locus_tag="PKI68_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53868.1"
                     /translation="MKALRILLALVLLSAAAALSVAVLIATNARAMEFVIRQVLARTA
                     PQVQEFRIGSLDYSFPASWTLERVQAAVPLQGRPVRLFANQLEIKDAQHLLKEGASMQ
                     LEVRGAEAAYEQLKVRGAACAVDLSRSASGLSYRGTASLETVLRSPLSLSDITAEFTG
                     NEQELVLNRLTAAVYGGRLSGSGRAVFGPPATYTAALEVQALDCAELEQALGGVFREL
                     GGKLSGQLQLAGSGRRIDEFDTAWSMPSGGAVSAELLSSIADYLPDSAQKKRIDFLIR
                     SGGKLAVESFQVTLKNDSPEQLSGIIGLKSREANLELNITHEIRVDARIDALLQAWQA
                     VFK"
     gene            complement(1575..>2458)
                     /locus_tag="PKI68_09010"
     CDS             complement(1575..>2458)
                     /locus_tag="PKI68_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53869.1"
                     /translation="LNVSREILQENPQLDKIRKNLTARILKTLGEMKLKSYDNYLSFY
                     NEFSPVLKEGLQTDFENREKIADLLLFQTTATEPGKMRSLADYVESMKADQTDIIYLA
                     AEHRGIAENSPFLEGFKADGREVLFLLDPIDEFVMPQLHEYKGKKFKAANKGDLHDAD
                     QLKDETKKFEGFFGFVKEKLGGIKEVRLSTRLKESPACLVGDEHSMSPHVEAMLRRMG
                     QPVPEMERVLELNPAHPVVAAVQSLYETDRENPKLAESVEVLRDLAHVAEGSKVENPS
                     AFAARIAALLARDLKPV"
BASE COUNT          424 a          792 c          728 g          514 t
ORIGIN      
        1 tcggccttgt cgcgctgaac ccgggcaaag acggtttcga cggtcgccag cgcagcggcc
       61 ccgagtttgt tctgctcaac aatcgcttcg tacaccagtt tgcggtcttt gttctccgcc
      121 gcgaccagcg cctgaacgtc cgcgccgccg cccagcgcct gcacataacc gcggttggtt
      181 tcgccgacaa cgccccgggt tttcagcgtc ttcagttcgg cgaagcgcga cttgcgtccg
      241 tcgagggcgg acttcaccgc cggcgtcatc agcttgatgt catacgcgga ctgggccggg
      301 gccgttgccg ccgccgtgca aatcaaaact aaaaccatga acagcttttt catcagtacc
      361 tccggttgtt acttcccgct gctaacatac tcttcgatgc cggacgccgt cttctccagc
      421 cctttaatgt cgatcgtgac atgcgcgttg atggtaatcg ggcggttggg gtcgccgacg
      481 gctttgaccg tgcagccggc gcagcacatc agtgcggcgg ccaaaaaaag atatttcata
      541 cgaacttcct ccgctctcat ttaaacaccg cctgccaggc ctgcagcagc gcatcgatcc
      601 gcgcatccac ccgtatttcg tgcgtgatat tcagctccag attggcttcg cggctcttca
      661 ggccgatgat gccgctcagc tgctccggcg aatcgttctt cagcgtgacc tgaaaggact
      721 ctaccgcaag cttcccgccg gaccggatta aaaaatcaat tctcttcttc tgcgcggaat
      781 ccggcagata gtcggcgatc gacgacagca gctccgcgct gaccgccccg cccgacggca
      841 tgctccaggc cgtatcaaac tcatcaatcc gccgtccgct tccggccagc tgcagctggc
      901 cggagagttt gccgcccagc tcgcgaaaaa ctccgcccag cgcctgctcg agttccgcgc
      961 aatccagcgc ttgcacctcc agcgccgccg tataggtggc gggcggaccg aaaaccgccc
     1021 gcccgctgcc ggacagccgg ccgccataaa ccgccgcggt cagccggttc agcacaagct
     1081 cctgttcatt accggtaaac tccgccgtga tgtccgaaag actcagcggc gaccgcaaaa
     1141 ccgtttccag actcgccgtt ccccggtagg aaaggccgct ggccgagcgg cttagatcaa
     1201 ccgcacaggc cgccccgcga actttcagct gttcatacgc cgcctctgcg ccgcgcacct
     1261 cgagctgcat cgacgcgccc tccttcagca ggtgctgggc gtctttgatt tccagctgat
     1321 tggcgaacag ccgcaccgga cggccctgca gcggcacggc ggcctgcacg cgctccagcg
     1381 tccagctcgc cggaaatgaa tagtccagcg agccgatccg gaactcctgc acctgcggcg
     1441 cggtgcgggc cagcacctgc cggatcacaa actccatggc gcgggcattg gtggcgatga
     1501 gcaccgcgac cgacagagcg gccgcggccg acagcagcac cagcgcgaga aggatccgga
     1561 gggctttcat ggcgttaaac cggcttcaaa tcgcgcgcca gcagcgccgc gatgcgcgcg
     1621 gcaaaggcgg acggattttc gaccttggag ccttcggcca catgcgcgag gtcgcgcagg
     1681 acttcgacgc tttcggccag cttcgggttt tcccggtcgg tttcgtagag cgactggacc
     1741 gccgcgacca ccggatgcgc cggattgagt tcgaggacgc gctccatttc cggaaccggc
     1801 tggcccatgc ggcgcagcat ggcttcgacg tgcgggctca tgctgtgttc gtcgccgacc
     1861 agacaggccg ggctttcttt caggcgcgtg gagaggcgca cttctttgat gccgccgagc
     1921 ttttctttga cgaagccgaa gaagccctcg aactttttgg tttcgtcttt gagctgatcg
     1981 gcatcgtgca ggtcgccttt gttggcggcc ttgaactttt tgcctttgta ttcatgcagc
     2041 tgcggcatga cgaactcgtc gatcggatcg agcaggaaga gcacttcgcg gccgtcggct
     2101 ttgaagcctt cgaggaacgg ggagttttcg gcgatgccgc ggtgttcggc cgcgaggtag
     2161 atgatgtcgg tctgatcggc tttcatgctt tcgacatagt cggccagcga gcgcatcttg
     2221 cccggttccg tggcggtggt ttggaacagc agcaggtcgg cgattttctc gcggttttcg
     2281 aagtcggtct gcaggccttc tttgagcacc gggctgaact cgttgtagaa gctcagatag
     2341 ttgtcgtagc tcttgagctt catctcgccc agcgtcttga ggatgcgggc cgtcaggttt
     2401 ttgcggattt tgtcgagctg cggattttcc tgcaggattt cgcgcgagac attcagcg
//
LOCUS       DAOPZO010000193         2448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_61739_length_2448_cov_8.373172, whole genome shotgun sequence.
ACCESSION   DAOPZO010000193 DAOPZO010000000
VERSION     DAOPZO010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61739_length_2448_cov_8.373172"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PKI68_09015"
     CDS             complement(<1..212)
                     /locus_tag="PKI68_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53870.1"
                     /translation="MKKTFIIMTVAFAAAFGVQAQQPGELAALQQQLNELSAKIAKLE
                     KANAEKTALAEQTAQVENANAAKIAQ"
     gene            583..1554
                     /gene="rpsB"
                     /locus_tag="PKI68_09020"
     CDS             583..1554
                     /gene="rpsB"
                     /locus_tag="PKI68_09020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNX53871.1"
                     /translation="MSQKNVTIQELLDAGLHFGHQTKRWNPKMKRFIHGAKGGIYIID
                     LQKTVVQLKLAQEFLSSVVGNGRRVLFVGTKKQARAVFQEAAEKAGQPYVIYRWLGGM
                     LTNNQTIRQSVKRMEELQALFADEVKLKAYPKQEQSVLRRELTKLERNLTGVKDMTSL
                     PGALLVIDICREKLAIAEAQRLGIPVVALVDTNADPDLVAYPIPGNDDSIRSLTLIAD
                     VLGGTIAEAHDAYVKAKAADDKRRKDEQEAERKVADAAREERKAKEDEEKKKRAKVLD
                     KLKKEKAAADKAKKDEAKAAKVKAEETPAPAAEAAAEAAPVEEPKAE"
     gene            1576..2415
                     /gene="tsf"
                     /locus_tag="PKI68_09025"
     CDS             1576..2415
                     /gene="tsf"
                     /locus_tag="PKI68_09025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNX53872.1"
                     /translation="MAEITASMVKELRDATNVSMMECKRALQEANGDKEKAFKLLRER
                     GMAVAAKKADRVAKDGKISAKVSDDGKTGVMIEINCETDFVTRNEIFQKFVVEMTDKA
                     MGASGDPAEVFKADIVAKVAEIGENIVLRKSEKYTVSGTGAVASYIHMGGKVGVLAEF
                     GFSKADTVNSAAFKELALDVCLHIAAARPASLDRSGVPAQLVEDEKALFAKQVEGKPA
                     NIVEGILKGKLNKFYSQICLLEQMFVKDDSMSITQLIEAKSKEIGDKVEIKRYTRWQL
                     GEA"
BASE COUNT          649 a          737 c          628 g          434 t
ORIGIN      
        1 atctgggcga ttttagccgc attcgcgttt tcgacctgcg cggtctgttc cgccaacgcg
       61 gttttttcgg catttgcctt ttcaagcttt gcgattttag cggacagctc gttaagttgc
      121 tgctgaagcg cagccagctc tcccggctgt tgcgcctgca caccgaacgc ggcggcaaag
      181 gccacagtca ttattatgaa tgttttcttc atgttctaac tctctccttt gtttcaggtt
      241 gcaccgccgg atttccctgc tgcccggcgg cctttttcca cgggcacttg ttcctgtctg
      301 ctggcagccc gttaagaata tgcgcacaat ctacggtgtc ttggttagga ttttgttagg
      361 cgcatgctag aaatccataa aagacacccg tccggcttaa ccggaccggc atcgcgatcc
      421 gccgatatcg ccaggaaaac cgcttgccag agaaggtttt cctgcgtata acctcgcccc
      481 ttaatagctt ccgcatgaag cggagctgaa gcgagtgttt tccggatgcc cccggaaagc
      541 caaagcggat tgttcaacaa agaactatag gaggggctgc acatgtctca gaaaaacgta
      601 acgattcagg aactgctgga cgcaggtctg cacttcggtc accagaccaa acgctggaac
      661 cccaaaatga agcgcttcat tcacggcgct aaaggcggga tctacatcat cgaccttcag
      721 aaaaccgtcg tacagctcaa gctggcgcag gaatttctga gcagcgtcgt cggcaacgga
      781 cgccgcgtac tcttcgtcgg caccaagaaa caggctcgcg ccgtgttcca ggaagccgct
      841 gaaaaagccg gccagcccta cgtcatctac cgctggctcg gcggtatgct caccaacaac
      901 cagaccatcc gccagagcgt caagcgcatg gaagaactcc aggctctctt cgccgacgaa
      961 gtgaagctca aagcctatcc gaaacaggaa cagtccgttc tgcgccgcga actcaccaaa
     1021 ctcgaacgca acctcaccgg cgttaaagat atgacctccc tgccgggcgc cctgctcgtc
     1081 atcgacatct gccgcgaaaa actggccatt gccgaagcac agcgcctcgg tattccggtt
     1141 gtcgcgctgg ttgacaccaa cgccgacccg gatctggtcg cctacccgat ccccggcaac
     1201 gacgactcca tccgctcgct cactctgatc gccgacgttc ttggcggcac catcgccgaa
     1261 gcgcacgacg cctacgtcaa agccaaagcc gctgacgaca agcgccgcaa agacgaacag
     1321 gaagcagaac gcaaagtcgc cgacgccgcc cgcgaagagc gcaaagccaa agaagacgaa
     1381 gaaaagaaga agcgcgccaa ggttctcgac aaactgaaaa aagaaaaagc cgcagctgat
     1441 aaagcgaaaa aagacgaagc gaaagctgcc aaagtgaaag cggaagaaac tccggctccg
     1501 gcagctgaag ccgccgcgga agctgctccg gtcgaagaac ccaaggctga gtaatctaat
     1561 tgagaggatc caacaatggc tgaaattaca gcaagcatgg tcaaagaact tcgcgacgcc
     1621 accaacgtca gcatgatgga atgcaaacgc gcactccagg aagccaacgg cgataaagaa
     1681 aaagccttca aactgctgcg tgaacgcggc atggccgtgg ccgccaaaaa ggcggaccgc
     1741 gttgccaaag acggcaaaat ctccgccaag gtttccgacg acggaaaaac cggcgtcatg
     1801 atcgaaatca actgcgaaac cgacttcgtt acccgcaacg aaatcttcca gaagtttgtc
     1861 gtcgaaatga ccgacaaagc gatgggagcc agcggcgacc cggcagaagt attcaaagcc
     1921 gacatcgtcg ccaaggtcgc tgaaatcggt gaaaatatcg ttctgcgcaa gagcgaaaaa
     1981 tataccgtct ccggcaccgg cgcagtcgcc agctacatcc acatgggtgg taaagtcggc
     2041 gtcctcgcag aattcgggtt cagcaaagcc gataccgtca acagcgcggc ctttaaagaa
     2101 ctggcgctcg acgtctgcct gcacatcgcg gcggcccgtc cggcatcact ggaccgctcc
     2161 ggcgttccgg cccagctcgt agaagacgaa aaagccctct tcgccaaaca ggtcgaaggc
     2221 aaaccggcca acatcgtcga aggcatcctg aagggtaaac tcaacaagtt ttactcccag
     2281 atctgcctgc tcgaacagat gttcgtcaaa gatgacagca tgagcatcac ccagctgatc
     2341 gaagcgaaaa gcaaagaaat cggcgacaaa gtcgaaatca aacgctacac ccgctggcag
     2401 ctcggcgaag cctaagcttc accgcctcga tgcacaaaag ccgttccg
//
LOCUS       DAOPZO010000194         2433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_62332_length_2433_cov_12.037847, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000194 DAOPZO010000000
VERSION     DAOPZO010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62332_length_2433_cov_12.037847"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PKI68_09030"
     CDS             complement(<1..209)
                     /locus_tag="PKI68_09030"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNX53873.1"
                     /translation="MNNQEREMTANVLKAMAHPIRLGVIELLADGEKTCTELFEHLEC
                     SQSMMSQQLKILSHQGLIVIRKEGT"
     gene            319..963
                     /locus_tag="PKI68_09035"
     CDS             319..963
                     /locus_tag="PKI68_09035"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNX53874.1"
                     /translation="MQTKQPVKKQSWKPGNMLSPVPAVLVSCGGTGDWKPNLITVAWA
                     GTVCTNPPMLSISVRPERHSYDIIRQTGEFVVNIPTVKQAKATDWCGVVSGRDHDKFE
                     KAGLTPAPALNVECPVVAECPVNIECKVRQTLELGSHVMFIAEVVGVQASESLIDAKG
                     KLCLENAGLFAYVHGAYFELGRYLGHFGFSVRKTKPAPTGKRSPHERTQRKKRT"
     gene            932..>2433
                     /locus_tag="PKI68_09040"
     CDS             932..>2433
                     /locus_tag="PKI68_09040"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNX53875.1"
                     /translation="MKEPSAKNGRETWSGQWGFILAAAGSAIGLGSIWRFPYITGENG
                     GGAFMLIYLLCCAAVGIPVMICEITLGRHTQRNPVGCFNQLTPPASLLAHLLGGGMVL
                     TGIALLCFHSIGWGITSLILGGLVFWKSWRIVGIMGVIAGFLILSYYSVVGGWAIGYV
                     AKAAGNQLTFETVAQAKTTYLDFIGSTGRVIGFHFVFMLLCVAIIYKGVQKGIETACK
                     FMVPLLFVIMLVLILRSLTLPGAAAGVSFCFTPDFSKLTAQGVLVALGHAFFTLSLGM
                     GAMITYGSYLGKKENIFASSVWITGLDTLVSILSCLMIFPAVFAMGFEPGEGPGLVFQ
                     VVPAVFHRLPAGPLWGTLFFILLAVAALASGISLLEVVTAYFVDEKKWTRHRAAIVTG
                     SAIFLLGGLSAVSIENWDKLPAIQKAVLACFGGARASFFDLVDNLTCNWMLPLGGLFI
                     CLFTGWIWGTRNAIGEIRHGSKNFADVHLLSLMAGLKDDPSHNTSTHPLTL"
BASE COUNT          465 a          817 c          636 g          515 t
ORIGIN      
        1 tgcgtgccct ccttgcggat cacaatcagc ccctgatggc tcaggatttt gagctgctgc
       61 gacatcatcg actgactgca ctccagatgc tcaaacagtt cggtgcaggt tttttcgccg
      121 tcggccagca gctcaatcac gcccagccgg atggggtgag ccatcgcctt gagtacattc
      181 gccgtcattt cccgttcctg attattcata agctcctccg aaaacattaa gaaattataa
      241 tgttctgatt ttaaaacaat caaaatcgcc ggttttctgc tggtctcgcc ggtggggtcc
      301 ggcttacaac acttttctat gcagaccaaa cagcccgtta aaaaacagtc gtggaaaccc
      361 ggcaacatgc tctcgccggt tccggcggtg ctggtcagct gcggcggaac cggggactgg
      421 aaaccgaatc tgatcaccgt tgcatgggcc ggaaccgtct gtaccaatcc gccgatgctc
      481 tccatttccg tccgcccgga acgccattcc tacgatatca tccggcagac cggcgagttt
      541 gtggtgaata tccccacagt caaacaggcg aaggccaccg actggtgcgg cgtcgtttcc
      601 ggccgcgacc acgataaatt tgaaaaagcc ggcctgacgc cggcgcctgc cctgaacgtt
      661 gaatgcccgg tcgtggcaga atgtccggtc aacatcgaat gcaaagtccg ccaaacgctg
      721 gaactcggtt cgcacgtcat gttcatcgcc gaagtcgtcg gcgtacaggc ttccgaaagc
      781 ctgatcgacg ccaaaggcaa gctctgcctc gaaaacgccg gcctcttcgc ctatgtgcac
      841 ggcgcctact ttgaactcgg ccgctatctc ggccacttcg gattctcggt tcgcaaaaca
      901 aaacctgcac cgaccggaaa aaggagtcca catgaaagaa cccagcgcaa aaaacggacg
      961 tgaaacctgg agcggccagt ggggttttat tctcgcggcg gcaggctccg cgatcggact
     1021 gggcagtatc tggcgctttc cctacatcac cggcgaaaac ggcggcggcg cgttcatgct
     1081 catctatctg ctctgctgcg ccgcagtcgg cattccggtc atgatctgcg aaatcacgct
     1141 gggccgccac acccagcgca acccggtcgg atgcttcaac cagctcacgc cgcccgcctc
     1201 gctgctggcc cacctcctgg gcggcggcat ggtcctcacc ggcatcgccc tgctctgctt
     1261 ccactccatc ggctggggca tcacgtcgct gattctcggc ggactcgtgt tctggaaaag
     1321 ctggcggatc gtcggcatca tgggcgtcat cgccggattc ctgatcctct cctactacag
     1381 cgtcgtcggc ggctgggcca tcggctacgt cgccaaggcc gccggcaacc agctcacctt
     1441 cgaaaccgtc gcacaggcga aaacaaccta tctggatttc atcggcagca ccggacgggt
     1501 catcggcttc cactttgttt tcatgctact ctgcgtggcc atcatctata agggcgttca
     1561 gaaagggatc gaaaccgcct gtaaattcat ggtgccgctg ctcttcgtca ttatgctcgt
     1621 actgatcctg cgctccctga cactgcccgg cgccgctgcc ggcgtcagct tctgcttcac
     1681 gccggatttt tccaaactca ccgcgcaggg cgtactggtc gcgctcggcc acgccttctt
     1741 caccctcagc ctcggcatgg gtgcgatgat cacctacggc agttacctcg gcaaaaagga
     1801 aaacatcttt gcctccagcg tctggatcac cggactcgac acgctggtct cgatcctctc
     1861 ctgcctgatg atttttccgg ccgtcttcgc catgggcttc gaaccgggcg aaggccccgg
     1921 cctcgtcttt caggtcgttc cggcggtctt ccaccggctt cccgccgggc cgctgtgggg
     1981 cacgctcttc ttcatcctgc tcgccgtcgc cgcgctcgcc tccggcatca gcctgctcga
     2041 agtcgtcacc gcctatttcg tcgacgagaa gaaatggacg cgccaccgcg ccgccatcgt
     2101 caccggcagc gccatcttcc tgctcggcgg actctccgcc gtcagcatcg aaaactggga
     2161 taaacttccg gcgatccaaa aagcggtcct cgcctgcttc ggcggcgcga gagccagctt
     2221 ctttgacctg gtggacaacc tcacctgcaa ctggatgctg ccgctcggcg gacttttcat
     2281 ctgcctcttc accggctgga tctggggcac ccgcaacgcg atcggggaaa tccgccacgg
     2341 ctcgaaaaat ttcgccgacg ttcatctact ctccctgatg gccggactca aagacgaccc
     2401 gtcgcacaac accagcaccc atccgctcac gct
//
LOCUS       DAOPZO010000195         2378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_64604_length_2378_cov_10.246664, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000195 DAOPZO010000000
VERSION     DAOPZO010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64604_length_2378_cov_10.246664"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..415)
                     /locus_tag="PKI68_09045"
     CDS             complement(194..415)
                     /locus_tag="PKI68_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNX53876.1"
                     /translation="MAAIKKGDLVKAVCGDDKGKTGKVLQVLPGTGRAVVEGLNFVKK
                     HMRKTQDKPQGGVVEKEASIHISNLKKAE"
     gene            complement(433..798)
                     /gene="rplN"
                     /locus_tag="PKI68_09050"
     CDS             complement(433..798)
                     /gene="rplN"
                     /locus_tag="PKI68_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNX53877.1"
                     /translation="MISVQTRLDVADNTGARSVQCIRVLKTKRSYAKVGEIIKVAVKE
                     AQPGGIVKKGEVCEAVVVRTKSAILRRDGSVLRFDHNAAVIIDAAKNPRGTRIFGPVA
                     RELREGNFMKVVSLAPEVL"
     gene            complement(813..1064)
                     /gene="rpsQ"
                     /locus_tag="PKI68_09055"
     CDS             complement(813..1064)
                     /gene="rpsQ"
                     /locus_tag="PKI68_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNX53878.1"
                     /translation="MESRGHRKVREGRVCSVAGNKTIAVLIERRVRHPLLGKELRVTK
                     KIHAHDENNEAKVGQVVRVMETRPISRLKNWRLVEILSK"
     gene            complement(1068..1268)
                     /gene="rpmC"
                     /locus_tag="PKI68_09060"
     CDS             complement(1068..1268)
                     /gene="rpmC"
                     /locus_tag="PKI68_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNX53879.1"
                     /translation="MKKVKELTDMTTVELQQQLEDAKKELFTLRLQQVAGQLENPLRI
                     RAVRRSVARIKTVMNQAAKAEG"
     gene            complement(1288..1707)
                     /gene="rplP"
                     /locus_tag="PKI68_09065"
     CDS             complement(1288..1707)
                     /gene="rplP"
                     /locus_tag="PKI68_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNX53880.1"
                     /translation="MPLMPKRVKYRKVQKGSRGGMAKAGTTLAFGEYGLQALERAWIT
                     NIQIEACRVAINRNLKRKGKVWLRIYPDKPFTKKPLEVRMGKGKGGVEGWVAVVRPGR
                     MLFELDGVTEQQARIAMRLAATKLPIRVRFVTRHANH"
     gene            complement(1720..2355)
                     /gene="rpsC"
                     /locus_tag="PKI68_09070"
     CDS             complement(1720..2355)
                     /gene="rpsC"
                     /locus_tag="PKI68_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNX53881.1"
                     /translation="MGQKVNPIGLRIAVNKNWRSRWFAGKKEFADLLQEDIAIREAVR
                     ARLKDAAISNVFIERYANRVRVTIRTARPGLVIGRKGEDIEKLREQLGKMTKKEVYIE
                     IDEVKNPDLDAALVAESIALQLERRVSYRRAMKRAIQMAMDIGADGIKVLASGRLGGA
                     EISRAESYKEGKIPLHTLRANIDYGTATARTVAGAIGIKVWICKKEEKAQA"
BASE COUNT          503 a          720 c          524 g          631 t
ORIGIN      
        1 agcaccgcct gcagcgcatc acggtcatgc tgcatgccga cggatacgtt aatattgatc
       61 ttcgaaagtg ccggtatctg catgcagttg ctgtagccga acttcttctg aagctcaggc
      121 acaacctgct cgcgatattt tttcttcatt gtcggtatca tggacaaccg ctcctctaaa
      181 taaaattaat gcgttactct gcttttttca gatttgaaat gtggatagaa gcctcttttt
      241 cgaccacgcc gccctgcggc ttgtcctgcg tcttacgcat gtgcttcttc acaaaattaa
      301 ggccttccac cacagcccga ccggtgcccg gaagcacctg aagaaccttg ccggttttcc
      361 ctttgtcatc accacagacg gctttgacca aatcaccttt ttttatcgct gccataaatc
      421 gttccctatc ccttaaagca cttccggggc gagcgagaca actttcatga agttgccttc
      481 gcgcagctca cgggccaccg ggccaaaaat acgcgttccg cgcggatttt tagctgcatc
      541 aataatgact gccgcattat ggtcaaaccg cagcaccgaa ccgtcacggc gcagaattgc
      601 cgatttggtc cggacaacta ccgcttcgca aacctcgcct tttttgacaa tgccgcctgg
      661 ctgagcctct ttgaccgcca ctttgattat ttcaccgacc ttcgcataac tgcgcttggt
      721 cttcaggacc cggatacact gaacgctccg ggcgcccgtg ttatctgcca catccagtct
      781 agtttgtact gaaatcattt tattatcctg ccttatttcg aaagaatttc gacaagccgc
      841 cagtttttaa gccgactgat cggacgcgtc tccatcaccc gcacaacctg accgacttta
      901 gcttcgttgt tttcatcatg tgcatgaatt ttcttcgtta cacgcagttc cttgcccagc
      961 aatggatgcc ggacgcggcg ctcgatgagc acagcgatgg ttttattgcc cgccacactg
     1021 cacacgcgac cctcgcgcac tttccgatga cctcttgact ccatctttta cccctcggct
     1081 ttcgccgcct gattcataac cgttttaatg cgagccaccg agcggcgcac agcacggatg
     1141 cgcagcggat tttccaactg ccccgcaacc tgctgaagac gaagcgtaaa cagctccttc
     1201 ttcgcatcct caagctgctg ctgcagctcg accgttgtca tatctgtaag ttcttttact
     1261 tttttcattc tatgctctca tctgaaatca atgattcgca tgacgcgtca cgaaccgcac
     1321 ccggatgggg agcttggtgg ccgccagccg catggcaatg cgggcctgct gttccgttac
     1381 gccgtcgagc tcaaaaagca tacgtccggg gcgaaccaca gccacccagc cttcgacgcc
     1441 gcccttccct ttacccattc ggacttccag aggctttttg gtaaacggct tatccgggta
     1501 aatccggagc caaaccttac ctttgcgttt caggttgcgg ttgattgcta cgcggcaggc
     1561 ctcaatctga atgttagtaa tccatgcgcg ctccagcgcc tgcagaccgt attcaccgaa
     1621 agccagtgtt gtaccggctt tagccatacc gccgcgggag cccttctgga ctttccgata
     1681 tttaactcgt tttggcatta aaggcattga tcaatactcc taagcttgcg ccttttcttc
     1741 cttcttacaa atccacactt tgatgccgat agcacccgct acggtacgtg cagtcgcagt
     1801 tccatagtcg atgttcgccc gcagtgtatg aagaggaatc ttaccttctt tataactttc
     1861 tgcgcgggaa atttcagcac cgccaagacg tccggaggcc agcaccttga taccgtccgc
     1921 accgatgtcc atcgccattt gaatggcacg cttcatcgca cggcgatatg aaacacggcg
     1981 ttccagctgc aacgcgatgc tttcagcaac cagcgcggca tccaggtcgg ggttcttcac
     2041 ttcgtcaatt tcgatgtaaa cttctttctt ggtcatcttg ccaagctgct cgcgcagctt
     2101 ttcgatgtcc tcgcccttgc ggccgatcac cagtcccgga cgggctgtgc gaatggtcac
     2161 gcggacacgg ttggcataac gttcaataaa aacgttggaa atcgcggcat ctttcagacg
     2221 cgctcgtaca gcttcacgga tagcgatatc ctcctgaagc agatcagcaa actctttttt
     2281 gccggcgaac cagcgtgaac gccagttctt atttaccgca atccggagtc cgataggatt
     2341 tactttctgt cccaaaacga ttccctttcc ttatttac
//
LOCUS       DAOPZO010000196         2352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_65703_length_2352_cov_4.777971, whole genome shotgun sequence.
ACCESSION   DAOPZO010000196 DAOPZO010000000
VERSION     DAOPZO010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65703_length_2352_cov_4.777971"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="PKI68_09075"
     CDS             <1..999
                     /locus_tag="PKI68_09075"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HNX53882.1"
                     /translation="EIIPAIRLIQTDSSSLAWATNRDLLASNPYSREFVVETQEDGVS
                     RLRFGDGLMGQKPDINLTFQALYRVGSGISGNVGAESLAHLVIRTDFGIAAGFDPAKI
                     KKVRNPLAARGGSEPESCEQIRKYAPQAFKIQERAVNAPDYAAIAMRHSDIQQAAATV
                     RWTGSWYTVYVTIDRKGGRSVDEAFIAEIKAYLDCYRMAGYDVEINGPRFVPLDIACN
                     VFVKPGYFKMDVKKVLVDAFSNRELSDGARGFFHPDNFTFGEAVYTSRIYAAAMAIDG
                     IGSIEIKKFQRLGKNAAGEIAKGRLSVGRLEVIRLDNDPNFPENGKIEFDMREANV"
     gene            1124..>2352
                     /locus_tag="PKI68_09080"
     CDS             1124..>2352
                     /locus_tag="PKI68_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53883.1"
                     /translation="MKADIARHPALRALATREDKDPSIALLDGCAAMLDVLTFYQERI
                     AEEGFLRTATERRSIGELAGQIGYQLAAGVAASVPLAFTVEDAPGAPGWAVIEKGTRL
                     QSIPGPQEKPQIFETMEKLEAHARFNRLKPKTSEVQKIRLDMTELYLKGTNLQLKQGD
                     ILLIVGDERQGYKLSENWDVRIVHTVTTDADQNNTIVTWLEPLGKRTSEKIVYPAQKN
                     PKVYVMRQRAALFGHNAPDPRILDVDSTLVAGTEWKSFAINQAAQTVDLDAPYPRILK
                     GGWIVLSKPGLIDDTYMEAYQINRLSLCSRKDFALSSDITRIVLDTGNNLDEFGLRNT
                     TVYAQSEELEMADVPFRSADGEETLNVRLGAGMLTPVEGSQITLDRYLPELQKGQLLI
                     VSGRPARLQSLTSSIFGL"
BASE COUNT          577 a          653 c          641 g          481 t
ORIGIN      
        1 gaaatcattc ccgccatcag gctcattcag actgattcct cctctctggc gtgggcaacc
       61 aaccgtgatc tcctcgcctc caatccctac agccgtgaat ttgtagtgga aacgcaggag
      121 gacggggttt cgcgtcttcg ctttggcgac ggcctcatgg gccagaagcc ggatataaac
      181 ctgaccttcc aggcgcttta ccgtgttggc agcggcatct ccggcaatgt cggcgcggag
      241 tcactggccc acctggtaat tcgcacggac ttcggcatcg ccgccggctt tgatccggca
      301 aaaatcaaaa aagttcgcaa tcctctggct gcacggggcg gctcagagcc tgaatcgtgc
      361 gaacagattc gcaagtatgc gccccaggcg ttcaaaatac aggaacgcgc cgttaatgct
      421 ccggactacg ccgccatcgc gatgcgccat tccgatattc agcaggccgc cgccaccgta
      481 cgctggacgg gaagctggta cacagtctac gtcaccatcg accgtaaagg cggcagaagt
      541 gtcgatgagg cgttcatcgc ggagataaaa gcctatctcg actgttaccg catggctggt
      601 tatgacgtcg aaatcaacgg gccgcgtttt gttcctctgg acattgcctg caatgtcttc
      661 gtgaagcccg gttattttaa aatggatgta aagaaagtgc ttgtcgacgc gttcagcaat
      721 cgcgaactga gcgacggcgc acggggattt ttccaccccg acaattttac cttcggcgag
      781 gccgtctata cgtcccgcat ctatgccgcg gccatggcca tcgatggaat aggctcgata
      841 gagatcaaaa aattccagcg gctggggaaa aatgccgccg gtgaaatagc aaagggcagg
      901 ctctccgtcg gccgcctgga agtcattcgc ctggataacg atcccaattt tccggaaaat
      961 ggaaaaatag aattcgacat gagggaagcc aatgtctgaa aattgtaaag attgtgggag
     1021 cactatcatc aaagccgaca tgacaccggt atccaaagaa cagcggcccg gcctcgcggc
     1081 gcttcgctac cgggttggca ctcacagcca atttcaagaa agcatgaagg ccgatatcgc
     1141 ccgtcatccc gcgctgcggg cactggcgac gagggaggac aaagatcctt ctattgccct
     1201 tttagacggc tgcgccgcca tgctcgacgt gctcactttt tatcaggaac gcatcgctga
     1261 agaaggattt ctgcgcacgg ccaccgaacg ccgctccatt ggcgaattag ccggtcagat
     1321 cggctatcaa ctggctgccg gcgttgcagc ctccgtaccc ttggctttta cggtggaaga
     1381 tgcccccgga gcaccgggct gggccgtgat cgaaaaagga acaaggctcc agagcattcc
     1441 cggcccgcag gaaaaaccgc agatttttga aactatggaa aaactggagg cgcacgcccg
     1501 gtttaaccgc ctgaaaccaa aaacaagcga ggtgcaaaaa attcgtctgg acatgacgga
     1561 gctttatttg aaagggacaa acctccagtt aaagcagggt gacattctgc tgatcgtcgg
     1621 cgatgaacgg caggggtata aactctcgga aaactgggat gtgcgcatcg tgcataccgt
     1681 gacgaccgac gccgatcaaa acaacacaat cgtcacctgg ctggagccgc tggggaagcg
     1741 cacttccgaa aagatagttt acccggcgca gaaaaatccc aaagtatatg tgatgaggca
     1801 gcgggcggcg cttttcgggc acaacgcacc ggatccgcgg attctggatg ttgacagcac
     1861 cctggttgcc ggaacggagt ggaagtcttt tgccatcaat caggccgctc aaacagtcga
     1921 cctcgatgcg ccttatccca gaattctgaa aggcggctgg atagtgcttt cgaagccggg
     1981 cctgattgac gatacttaca tggaagccta tcaaatcaac agattgagcc tgtgttcacg
     2041 caaggacttt gccctgtctt ccgacattac ccgcattgtc ctggatacag gcaacaacct
     2101 ggatgaattc ggcctgcgca acaccaccgt ttatgctcaa agcgaagaac tggaaatggc
     2161 cgatgttcct ttccgttccg ccgacggcga agaaacctta aatgtcaggc tcggcgccgg
     2221 catgctgact cccgtggagg gttcgcaaat tacgctggat cgatacctgc cggaattgca
     2281 gaaaggacaa ctgctgattg taagcggcag gcccgcgcga cttcaaagcc tcacgagcag
     2341 cattttcgga ct
//
LOCUS       DAOPZO010000197         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_67380_length_2313_cov_9.281665, whole genome shotgun sequence.
ACCESSION   DAOPZO010000197 DAOPZO010000000
VERSION     DAOPZO010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67380_length_2313_cov_9.281665"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..779)
                     /gene="lptB"
                     /locus_tag="PKI68_09085"
     CDS             complement(48..779)
                     /gene="lptB"
                     /locus_tag="PKI68_09085"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNX53884.1"
                     /translation="MDETYLIRTEKLVKSYRGRTVVNEVDVNVRPGEIVGLLGPNGAG
                     KTTTFYMVVGLIRPTAGEVWFKDRNVSQVPMYKRARMGLGYLSQEPSIFRKLTVQENV
                     MAILETLPISGRERRDRLDFLLNELKISHLAKQRAYTLSGGERRRLEITRALVTNPSL
                     ILLDEPFSGVDPLAVYEVQEIIKDLRSKGLGVLITDHNVRETLAVVDRAYLMCEGKVL
                     REGASSFLVNDEMSRELYLGPRFSM"
     gene            complement(781..1689)
                     /locus_tag="PKI68_09090"
     CDS             complement(781..1689)
                     /locus_tag="PKI68_09090"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HNX53885.1"
                     /translation="MRKNSTYRSAVRFLLILLAPMAYSQMAGLELSSFRVPEYDAKGV
                     MTSQLFGEHAVIGDDGEVNITGLIIEFYKDSNTVMKVTSPYCFYNQKTKEAHSDAPVA
                     GDMDRMHVRGQGYFLKPGENTVRILNQSQVTIEGIMQKVNTNAPSSGNRATNAIPGLS
                     ALTGKRGSNDVTVITSKELFLNYKAKNVRFEQNVHVQDPKMTLDCGTLDVRFGEKNEI
                     NWIEALTGVKIVSEGREADAGKAVYDVKTDEFVLEDNPSFRDGRNKLYGDRIRFWRAS
                     GRMVCEPRARLVIYPDKNVKTDFFGK"
     gene            complement(1694..>2313)
                     /gene="lptC"
                     /locus_tag="PKI68_09095"
     CDS             complement(1694..>2313)
                     /gene="lptC"
                     /locus_tag="PKI68_09095"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNX53886.1"
                     /translation="AQEQGMELSVFRVPEYDDQGNMTAQLFGRHAEVGLSGEVKIEKM
                     RIEFYQAGATYVTVESPLCFYNAQKRAAHSDSEVSAVAEKIKATGRGFEWDPATHSIR
                     VFNDVHVVLENTAGLKSSLLSSGLTNSIVSGGAVSTATGGVVTVTSKELLLDYIAKIV
                     QFEKGVNVTSSDGTEMHCEKLKIPLNEGRTAGVLKLPGMTEPDAE"
BASE COUNT          523 a          712 c          525 g          553 t
ORIGIN      
        1 acatgccgtc ctgtgatatg cacttgcatg agcttctcct ttctctgtta catactgaac
       61 cgcgggccta gatacagctc gcggctcatc tcgtcattaa ccaaaaatga actggcgcct
      121 tcgcgaagca ccttgccttc acacatcaga tacgcacgat ccaccaccgc cagcgtttca
      181 cgcacattat ggtcggtaat cagcacgccg agtcccttgg aacgcagatc tttaataatc
      241 tcctgcactt cataaaccgc cagcggatcg acgccgctga acggctcatc aagcagaatc
      301 agcgacggat tggtcaccag tgcacgggta atctccagcc gccggcgttc gccgccggaa
      361 agagtatagg cgcgctgttt ggccagatgg gaaattttca gctcgttcag cagaaaatcc
      421 agccggtcgc ggcgttcgcg gcccgaaatc ggcagcgttt caagaatcgc catcacgttt
      481 tcctgaaccg tcagcttgcg gaaaatcgaa ggttcctgag acagataccc cagtcccatc
      541 cgggcgcgct tatacatcgg cacctgggaa acattgcggt ctttgaacca gacttcaccg
      601 gccgtcggac ggatcagtcc gacaaccata taaaaggtcg tcgtcttgcc cgcgccgttc
      661 gggccgagca gtccgacgat ctcgccggga cggacgttca catccacttc attcaccacg
      721 gtccgcccgc ggtaactctt caccagtttt tcagtccgga ttaaataggt ctcgtccatt
      781 tcacttccca aaaaagtcag tcttcacatt tttatcagga taaatcacca gccgcgcccg
      841 cggctcgcac accatccgtc cgctggcacg ccagaaacgg atacggtcac cataaagctt
      901 attccttcca tcccggaaac tcgggttgtc ctccagcacg aactcatcag ttttaacgtc
      961 atagacggct ttacctgcat ctgcctcccg cccttcgctg acgattttaa cgccggtcaa
     1021 agcctcgatc cagttaattt catttttttc accgaaacgt acatccagcg tgccgcaatc
     1081 cagcgtcatc tttggatcct gaacgtgaac gttctgttca aaccggacat ttttggcttt
     1141 gtagttaagg aaaagttctt tggaggtgat aaccgttaca tcgttgcttc cgcgctttcc
     1201 ggtcagcgcg ctcagacccg gaatcgcatt ggtcgcgcgg tttccggatg aaggcgcgtt
     1261 cgtattcacc ttttgcatta ttccttcaat cgtcacctgg ctctgattta aaatccgcac
     1321 cgtattttca cccggcttca gaaaataacc ctgaccgcgc acatgcatcc ggtccatgtc
     1381 gccggcaacc ggcgcgtccg aatgtgcttc cttcgttttc tggttataaa aacagtaagg
     1441 cgaagtcacc ttcatcaccg tattgctgtc tttgtaaaat tcgatgatca gtccggtaat
     1501 gttcacttct ccgtcgtctc cgataacggc gtgctcaccg aacagctgcg aagtcatcac
     1561 acccttcgca tcatattcag ggacgcggaa cgaggacagc tccagaccgg ccatctgcga
     1621 atatgccatc ggcgccagca ggatcagcag aaagcggact gcgctccggt atgtactgtt
     1681 ttttctcata acatcactcg gcatccggtt ccgtcattcc cggcagtttc aaaacaccgg
     1741 ccgtgcggcc ctcattcagc ggaattttca gtttttcaca atgcatttcc gttccgtcac
     1801 tgctcgtcac attcacacct ttttcaaact gcacgatttt cgcgatgtaa tcaagaagca
     1861 gctccttcga tgtaaccgtg accactccgc ctgtagcagt cgaaaccgca ccgccggaaa
     1921 caatggaatt tgtcagcccg ctggaaagaa gagaactctt caacccggcg gtattttcca
     1981 gaacgacgtg aacatcatta aaaacccgta tgctgtgcgt cgccggatcc cactcaaaac
     2041 cgcggcccgt cgctttgatt ttttcggcga cggcggaaac ttcggagtcg gaatgagccg
     2101 cgcgcttctg ggcattataa aaacagagcg gcgactcgac cgtcacatac gttgccccgg
     2161 cctgataaaa ttcaatccgc atcttctcaa tcttcacctc gccggacagg ccgacctcgg
     2221 catgccggcc gaaaagctgc gccgtcatat tgccctgatc gtcatattcc ggcacgcgga
     2281 agacggacag ttccatgccc tgctcctgcg cag
//
LOCUS       DAOPZO010000198         2234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_71131_length_2234_cov_7.540615, whole genome shotgun sequence.
ACCESSION   DAOPZO010000198 DAOPZO010000000
VERSION     DAOPZO010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71131_length_2234_cov_7.540615"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /gene="murA"
                     /locus_tag="PKI68_09100"
     CDS             <1..1304
                     /gene="murA"
                     /locus_tag="PKI68_09100"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNX53887.1"
                     /translation="RKMTIVCAMAKFIINGGNLISGKFSPRGNKNAVLPMLAACTLTD
                     QPVRLCNVPDIADVRVMLELLMSLGVSVEKSGGCVTLCAKGLKATELDEEYCRKVRTS
                     ILLAGPLTARHGSALIHSPGGDVIGRRRLDTHFYGLRALGIEVNDTAPYHFKRKALVG
                     ADLILDEASVTATENILMAAVLAEGETSIFNAACEPHVQDLAHLLVKMGADIAGIGTN
                     RIHVRGVKLLAGAEHTIQPDYIETGSFLTAAAVTGGALEIDAPGDTVTLNVLEKGFSK
                     LGVKWTLKDGVLTMPPLKNRQIVPDLGNFTPKIEDGIWPAFPSDLMSVMIVLATQTQG
                     QMLFFEKLFESRMYFVDNLMAMGANIIQCDPHRVVVSGAAKLRGARLTSPDIRAGMAM
                     LIAACCAEGRSTIDNAQMIDRGYEAIDERLRALGADITREK"
     gene            complement(1365..1637)
                     /locus_tag="PKI68_09105"
     CDS             complement(1365..1637)
                     /locus_tag="PKI68_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53888.1"
                     /translation="MRIVFEILGFGLLVLVAGVLILLLAGLYDFTAEPDGDLQLWIGV
                     GVNLLSTVILEIVFFGPRALKKYREKRAQLAGIVEQEIQSVMDEAT"
     gene            complement(1934..>2234)
                     /locus_tag="PKI68_09110"
     CDS             complement(1934..>2234)
                     /locus_tag="PKI68_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNX53889.1"
                     /translation="DAVHKAAADEMEKRGYKTKTIDGVPQGFIHGTGHGVGLEIHEAP
                     RVSSAVHKKLRAGNIITIEPGLYYPHIGGIRIEDTIAVTKDGHQMLASCPKKLEI"
BASE COUNT          487 a          667 c          601 g          479 t
ORIGIN      
        1 tcaggaaaat gactattgtc tgcgccatgg caaaattcat catcaacggc ggtaatctca
       61 tcagcggaaa attctctccg cgcggcaata aaaacgcagt cctgcccatg ctggcggcct
      121 gcaccctgac cgatcagccg gtccggcttt gcaacgttcc ggatatcgcc gacgtgcgcg
      181 tcatgctcga actgctcatg tcgctcggcg tatcggtgga aaaatccggc ggctgcgtaa
      241 cgctctgcgc caaaggtctt aaagccaccg agctcgacga agaatactgc cgcaaagtgc
      301 gcacctctat tctgctggcc ggcccgctga ccgcgcgtca cggctcggcg ctgattcatt
      361 cgcccggcgg cgacgtcatc ggccgccgcc gtctcgacac ccatttctac ggactgcgcg
      421 cgctcggcat cgaagtcaac gataccgcgc cgtaccactt taagcgcaaa gcgctggtcg
      481 gcgccgacct gattctcgac gaagccagcg tcaccgccac cgaaaacatc ctgatggccg
      541 ccgtgctggc cgaaggcgaa acctccatct tcaacgccgc ctgcgaaccg catgtacagg
      601 atctggcgca tctgctcgtc aaaatgggcg cggatatcgc cggaatcggc accaaccgga
      661 tccatgtccg cggcgtcaaa ctgctcgccg gagcggaaca caccattcag cccgactata
      721 ttgaaaccgg aagtttcctc accgccgccg ccgtcaccgg cggcgcgctc gaaatcgacg
      781 cgccgggcga caccgtcaca ctcaacgtcc tcgaaaaggg gttctccaaa ctcggcgtta
      841 aatggacact gaaagacggc gtcctgacga tgcccccgct caaaaaccgc cagattgttc
      901 cggatctcgg caacttcacg ccgaaaatcg aagacggcat ctggcccgcg tttccgtccg
      961 acctgatgag cgtgatgatt gttctggcca cgcagacgca ggggcagatg ctctttttcg
     1021 aaaagctgtt cgaaagccgc atgtacttcg tcgacaacct catggcgatg ggcgccaaca
     1081 tcatccagtg cgacccgcat cgcgtcgttg tcagcggtgc ggccaaactg cgcggcgcgc
     1141 gcctgaccag tccggacatc cgcgccggta tggcgatgct gattgccgcc tgctgcgccg
     1201 aagggcgcag cacgattgat aacgcgcaga tgatcgaccg cggctacgaa gccatcgacg
     1261 aacgcctccg cgccctcggc gccgatatta ctcgggaaaa gtgatcagaa acgccgagtt
     1321 gagccgtagc cgtgatttag tggctgtcgc gctttaactt ggtgttaggt agcctcgtcc
     1381 attacagact gtatctcctg ttcaacaatt cctgctagtt gtgcgcgttt ctcacggtat
     1441 ttctttagtg cacgggggcc aaagaagaca atttctagaa taaccgtaga caggagattc
     1501 acaccaacac cgatccaaag ttgtaggtca ccatcgggtt cggcggtgaa gtcgtacagt
     1561 cctgcaagca aaagaatgag gacgccagcg accaaaacca gaagtccgaa gccgaggatt
     1621 tcaaaaacaa ttctcaacgg tattttctgc gaagttttgt agccctttct ccgcggagcg
     1681 tttgagacaa cctctgtctc tttgttgttt cgtcactcaa taggttcttc gtaattgagt
     1741 ctgctcgtta agacagggcc ggctggaagc cggcgatacg taccgccggc ttccagccgg
     1801 ccttgatcaa acactaaata tcgtccgcaa ctgtgaacaa gcgaagcgcg ctcggcggtc
     1861 gcgctccacc tgcaccaact atcaattcat tacaaattga tagttggtcg ataagttaaa
     1921 acccttggga aaatcaaatt tccagcttct tcgggcatga ggcgagcatt tgatgcccgt
     1981 ctttggtgac agcgatggtg tcttcgatgc ggatgccgcc gatatgcgga tagtaaagtc
     2041 cgggttcgat ggtgatgatg tttccggcgc gcagtttttt atgtactgcc gatgaaacgc
     2101 gcggcgcttc gtggatttca agtccgacgc cgtggccggt tccgtgaata aatccctgcg
     2161 ggacgccgtc gatggttttg gttttgtagc cgcgcttttc catttcatct gcggcggctt
     2221 tatgaaccgc atcg
//
LOCUS       DAOPZO010000199         2208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_72364_length_2208_cov_8.942406, whole genome shotgun sequence.
ACCESSION   DAOPZO010000199 DAOPZO010000000
VERSION     DAOPZO010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72364_length_2208_cov_8.942406"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1715
                     /locus_tag="PKI68_09115"
     CDS             228..1715
                     /locus_tag="PKI68_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNX53890.1"
                     /translation="MKIKLPPFSLPGKTVACIITSLLTAAHAMAVEAPKPFGPVPTER
                     QLAWHELDFYAFIHFTVNTFTDKEWGYGDEAESVFNPTDFSAEQIVQVSKDAGMKGVV
                     LTAKHHDGFCLWPSAFTEHSVKNSSWKDGKGDVVREIADACRKQGLKFGVYLSPWDCN
                     RADYGKPEYITYFRNQMRELLTNYGPVFEVWIDGANGGKGFYGGAKEKRTIDRLTYYD
                     WPNTISIVRELQPGAVMFSDAGPDVRWAGNERAIAGDPCWATLNRADFSPGQSDRKRL
                     NTGDRPGTDWVPAECNTSIRPGWFYHEKEDKEVKTPVQLVDLYFKSVGRGGSFILNLP
                     PDRRGRIADPDINSLKEFRKRIDAIFATNLTAGAKVTASNVRGNDKTFAAENVLSGNR
                     TNYWSTDDTVTTPELILDLGRPKTFNIVSLREFLPLGQRIEEFALDQWKDGQWVQFAT
                     GTSIGNRRLIRGAFVTTSKVRLRITKAPVCPAITEFGLYAEPAAH"
     gene            complement(1854..2105)
                     /locus_tag="PKI68_09120"
     CDS             complement(1854..2105)
                     /locus_tag="PKI68_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HNX53891.1"
                     /translation="MSQELQPDHEAFIKLANAVMPFGKYAGRRLVDLPEPYVVWFKQK
                     GFPKGELGQMLETVYTIKANGLEYLFRPGAKNPEKKGRL"
BASE COUNT          502 a          627 c          603 g          476 t
ORIGIN      
        1 cgcgctttga ttttctccag ccccggtttc acggcacgca gcgcttctgc tgtgaaccgc
       61 ttatcgtccc gccggtgatc gccctcttca aacaccggcg gattttcaat taccgtttaa
      121 tcgccggaaa aaaggttgtt cagcgaggat ttgtatagca aagtgcagaa ttgcttctcc
      181 gggagggaag cttgatcgtg ttacacaaat tgactggaaa gatggatatg aaaattaaat
      241 taccaccatt cagcctgcct ggaaaaactg ttgcatgcat tattacttca cttctgaccg
      301 cagcccatgc gatggctgtc gaggcgccga aacccttcgg tccggtgccg acggagcggc
      361 agctcgcgtg gcatgaattg gacttttacg cgtttattca tttcaccgtc aatacgttca
      421 ccgacaagga atggggctac ggcgacgagg cggaatccgt tttcaatccg actgatttca
      481 gcgcagagca aattgtgcag gtctcaaaag atgccggcat gaagggagtg gttttgacgg
      541 ccaagcacca tgacggtttt tgtctctggc cgtcggcgtt cacggaacat tccgtgaaaa
      601 attcgtcctg gaaagacggc aaaggcgatg tggtccgtga aatagccgac gcctgccgca
      661 agcaggggct caagttcggc gtctatctgt cgccatggga ttgcaaccgg gccgattacg
      721 gcaagccgga atatatcacc tacttccgca accagatgcg cgaactgctg acaaactacg
      781 gcccggtttt cgaggtgtgg atcgacggcg cgaacggcgg caaaggcttc tacggcggcg
      841 cgaaagaaaa gcggacgatt gaccggctga cgtattatga ctggccgaac accatttcca
      901 tcgtccgcga attacagccg ggcgcagtga tgttcagcga cgccgggccc gacgtgcgct
      961 gggccggcaa cgagcgcgcc atagcgggcg atccgtgctg ggcgactctg aaccgcgcgg
     1021 atttcagccc gggtcagtcg gacagaaaac gtctgaacac gggcgatcgt cccggcaccg
     1081 attgggttcc cgccgagtgc aatacatcca tccgccccgg ctggttttac catgagaaag
     1141 aggacaaaga ggtcaaaaca ccggtgcagc tggtggacct gtatttcaaa tccgtcggac
     1201 gcggcgggtc gttcatactg aatcttccgc cggaccggcg cgggcgtatc gctgatcccg
     1261 atatcaactc cctgaaagag ttccgcaaga gaatcgatgc catcttcgca acaaacctga
     1321 cggccggcgc caaggtgacc gccagcaatg tgcgcgggaa cgacaaaacg ttcgcggccg
     1381 agaatgttct ttccggcaac agaacaaatt actggagcac ggacgacacc gtgacaacgc
     1441 cggaactgat tctggatttg ggccggccga aaacgtttaa catcgtcagc ctgcgcgaat
     1501 ttctgccgct cggccagcgc atcgaagagt tcgcactgga ccagtggaaa gacggacaat
     1561 gggtccaatt cgccaccggc acgagcatcg gaaaccgtcg gttgattcgc ggcgcgtttg
     1621 ttaccaccag caaagttcgc ctgcgcatca ccaaagcgcc cgtctgtccc gccatcacag
     1681 agttcggatt gtatgcagag ccggcggctc attgagagac ccgttaagct gcatccaaca
     1741 cctttccgct gattactacg gcgcagctgc ttgcggtgac tcaagcatct gccgccgaat
     1801 ccactttgac tgattctgac gcgggttgct gctgagcggc aacccgtttc aaatcaaagc
     1861 cgccctttct tttcggggtt tttcgcgccg ggacggaaaa gatattccag cccgttggct
     1921 ttgatcgtgt aaacggtttc aagcatttga cccagctcgc ccttcgggaa ccctttctgt
     1981 ttaaaccaga caacatacgg ttccggcaga tcaaccagcc gccgtccggc atatttaccg
     2041 aaaggcatca ccgcgttcgc cagcttgata aaggcttcgt gatcgggctg aagttcctga
     2101 ctcatgcgcc ggattctgat ttattttccg gaaacttcat acgcaaaaat cacttcgccg
     2161 cccgcctcaa ccggtctcac gttttaaatt tccggttgac cggtgaac
//
LOCUS       DAOPZO010000200         2200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_72815_length_2200_cov_8.850350, whole genome shotgun sequence.
ACCESSION   DAOPZO010000200 DAOPZO010000000
VERSION     DAOPZO010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72815_length_2200_cov_8.850350"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..1681
                     /gene="tig"
                     /locus_tag="PKI68_09125"
     CDS             386..1681
                     /gene="tig"
                     /locus_tag="PKI68_09125"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNX53892.1"
                     /translation="MKVTMKSTGPCRQTIKIEVPAETVAAERAELLTYYTKGVSLPGF
                     RKGHAPKALVEKKFAKDMAADLKDRVVPKFYHEAIAQEKVKVVAVVDVSEPELADGKP
                     LKFEVTVDVPPEFKLPKYDGISIKAESTEVTEKQIDETVESILRQHATYEDITGRAAK
                     EKDMVQVSYESTIGGQPLEEKVPQARGLGRGNGYWITCDDESFLPGMGKALIGAKIGG
                     KKDVTVKFPEGFIVKELAGMKAEFKVEVTGMRETKLPELNEEFLKKLHIESEAELRKN
                     IREHMEEAAKNKEKGRQQDEICEFLLKKTKLDVPESAVKEQTRNLTYELARQRMMRGM
                     SQEQIKAQSEDMFKEAQEKAEEQVKLRYIVMAVADAENIKVDDQEVSDEVVRMAVSQR
                     QDAAEYRKKLEKEDQLESVRDQIRFGKTIEFLLENAKIK"
     gene            1800..>2200
                     /locus_tag="PKI68_09130"
     CDS             1800..>2200
                     /locus_tag="PKI68_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002869062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNX53893.1"
                     /translation="MLVPMVVEQTGRGERAYDIYSRLLKERIIFLGTEINDDVSNLVI
                     AQLLFLQSEDAEKDISIYINSPGGVVTAGMAIYDTMQFVKCPITTYCMGQAASMGAVL
                     LAAGTKGKRFALPNSRIMIHQPLGGAQGQAT"
BASE COUNT          568 a          585 c          658 g          389 t
ORIGIN      
        1 aaaaggtagg gcgcgaccgc cgggcgcgcc gccagtccta tgtgacggac ggctcggcga
       61 tccgtcccta cctgaacgtg cgaggccctt cgacgagctc aggacaggcg atccgttcct
      121 gccaaaatcc gcgcccgacg gacgcggcta cagtttttcc tgtagatgcg gccggcggcc
      181 gcgttcttca aaggtggaac gcgccgtccc ggcgcgtttg aattaagaag cgtgccggga
      241 cggcccgctc caccaaagcg gcatcctgcc gccggccagg gccgggctgt ccagcctacc
      301 ttttccaacc ttggaaattg acctcgccgg aggggtgccg tattctgtgc cgcttttaga
      361 tttccaataa aacaggagaa ttttgatgaa ggttacaatg aaaagcaccg ggccgtgccg
      421 ccagacgatt aaaatcgaag tgccggctga gactgtagcc gccgagcgcg ccgaactgct
      481 gacctattac accaagggcg tcagcctgcc ggggttccgt aaaggtcacg cacctaaagc
      541 gctggtggaa aagaagtttg ccaaggatat ggccgccgat ctgaaggatc gcgtggtgcc
      601 gaagttctat cacgaagcga tcgcgcagga aaaagtcaaa gtggtcgcgg tggtggatgt
      661 cagcgagccg gaactggccg acggcaagcc gcttaaattt gaagtgacgg tggacgttcc
      721 gccggaattc aagctgccga agtacgacgg catttccatc aaggccgaaa gcacggaagt
      781 gaccgaaaag cagatcgacg aaacggtcga gagcatcctc cgtcagcacg cgacctacga
      841 agacatcacc ggccgcgccg ccaaagaaaa agatatggtg caggtttcct acgaatcgac
      901 catcggcggt cagccgctgg aagaaaaggt tccgcaggcc cgcggtctcg gccgcggcaa
      961 cggttactgg atcacctgcg acgacgagtc gttcctgccg ggaatgggca aggctctgat
     1021 cggcgcgaag atcggcggca aaaaagatgt caccgtgaaa ttcccggaag ggttcatcgt
     1081 caaggaactg gccggcatga aggctgagtt caaagttgaa gtgaccggta tgcgcgaaac
     1141 caagctgccg gagctcaacg aagagtttct gaaaaaactt catatcgaat cggaagcgga
     1201 actgcgcaag aacatccgcg agcacatgga agaagcggcg aagaacaagg agaagggccg
     1261 tcagcaggat gaaatctgcg aatttctcct taagaagacc aagctcgacg ttccggaaag
     1321 cgccgttaaa gaacagacgc gcaacctgac ctatgaactg gcccgtcagc gcatgatgcg
     1381 cggcatgagc caggagcaga tcaaggcgca gagcgaagac atgttcaagg aagcccagga
     1441 aaaggctgaa gagcaggtga agctgcgcta catcgtcatg gcggttgccg acgctgagaa
     1501 cattaaagtc gacgatcagg aagtgtccga cgaagtggtc cgcatggcgg tttcacagcg
     1561 tcaggatgcc gctgaatacc gcaagaagct ggagaaggaa gaccagctgg aatcggtacg
     1621 cgaccagatc cgcttcggaa aaaccattga gttcctgctc gaaaacgcca aaattaaata
     1681 gaaatctgat attcgatatt tgttatttga gggagagttg cagagataag agcgtatttt
     1741 tcccaataac gaatagccca taacaaataa cggagtttct tatgaacgaa agggcacaaa
     1801 tgcttgttcc gatggtggtt gaacagacgg ggcgcggtga acgcgcctat gacatttatt
     1861 cgcgtctgct caaggagcgg atcattttcc tcggcacgga aatcaacgat gacgtgagca
     1921 atctcgtcat cgcgcagctg ctgttcctgc agagcgaaga cgcggaaaaa gacatcagca
     1981 tctacatcaa ctcgcccggc ggtgtcgtca cggccggcat ggcgatctat gacaccatgc
     2041 agttcgtaaa gtgcccgatc accacctact gcatggggca ggctgccagc atgggcgcgg
     2101 ttctgctggc cgcgggaacc aagggcaaac gctttgcgct gcctaactcg cgtatcatga
     2161 tccatcagcc gctcggcggc gcgcagggtc aggccaccga
//
LOCUS       DAOPZO010000201         2147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_75525_length_2147_cov_8.579350, whole genome shotgun sequence.
ACCESSION   DAOPZO010000201 DAOPZO010000000
VERSION     DAOPZO010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75525_length_2147_cov_8.579350"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PKI68_09135"
     CDS             complement(<1..259)
                     /locus_tag="PKI68_09135"
                     /inference="COORDINATES: protein motif:HMM:NF023549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
                     /protein_id="HNX53894.1"
                     /translation="MGIQTILGIFLLVVVFVFAAVILSVLAVWVQAWGSGAPIKLYNL
                     IGMKVLRKVPVTLIARARIQAIKAGIDISSDLLEAHYLARGN"
     gene            complement(281..1435)
                     /locus_tag="PKI68_09140"
     CDS             complement(281..1435)
                     /locus_tag="PKI68_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53895.1"
                     /translation="MKPEIRNLKLGLIAGLLLVGSTVQAIEFVQRDQFISGESETLRD
                     EMWISAQDITISGEALDDLFAAGTTLDLRGNFKGDVWAGGGNQVIAAGRFGDHVRLVS
                     RMVQASGTFGNSLAAAGNTIKIESSASIAKNLFGAGDNVISEGAIAGNVRIVAKKATL
                     GGKIAGNVSIAAQEIVILPGTVIGGDLNYTAPKELVLSPSIVLGGKLNRTFEAPPANQ
                     FLKPNLFSHFVFAFAALLTGLVFCAVFPRYAANTFQLLRTSRGACILTGFTALCLIPI
                     GAFVLLFTLIGLPLSILLILFYLILLYLSKIAVGLWLGALVLRRSKEFSKRNLPGTLA
                     AGLFIIYALTAFTAISMVISLLVMIYGLGALILALFKKPVLVFQTPNDIK"
     gene            complement(1425..1895)
                     /locus_tag="PKI68_09145"
     CDS             complement(1425..1895)
                     /locus_tag="PKI68_09145"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNX53896.1"
                     /translation="MSWYITLLVCGLFLIGVEIFIPGGIVGAAGAAALIGAAVMGFMI
                     FPLWLGWLSLFFILVLTAIAAFVWMKYFPKSRMGKALSLTQHISKRDQDDSTWKAGMR
                     GTALSILRPAGKALIEGRRADVIADGTWIEQNSAIEIIKVEGNRIYVRKVKLET"
     gene            complement(1988..2064)
                     /locus_tag="PKI68_09150"
     tRNA            complement(1988..2064)
                     /locus_tag="PKI68_09150"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2028..2030),aa:Arg,seq:cct)
BASE COUNT          518 a          597 c          584 g          448 t
ORIGIN      
        1 cgttgccgcg cgccagatag tgcgcttcga gcaggtcgct gctgatatca attcccgcct
       61 tgatggcctg aatgcgcgct cgggcgatca gcgtgaccgg cactttgcgc agcactttca
      121 ttccgatcag gttgtacagt ttgatgggcg cgcccgaacc ccaggcctgc acccagaccg
      181 cgagtacgga cagaatgacg gccgcgaaga caaagacgac gaccagcaga aaaattccta
      241 atatcgtttg gattcccatg acctaaccct ccattgttgt ttacttgata tcgtttggtg
      301 tctgaaagac cagcaccggt tttttgaaaa gagcgagaat cagtgcgccg agtccgtaga
      361 tcatgaccag aaggctgatg accatggaga tcgcggtaaa ggctgtcagc gcataaatga
      421 taaacagtcc ggcggccagt gtgccgggca gattacgctt cgaaaattcc ttgctgcggc
      481 gcagaacgag ggcgccaagc cagaggccga ccgcgatttt gctgagatag agcagaatga
      541 gatagaagag gatcagcaga atgctcagcg gcagtccgat gagggtgaag agcagcacga
      601 atgcacctat cggaatgagg cagagtgcag tgaagccggt aagaatacat gcgccgcggg
      661 aggtgcgtaa cagctggaat gtgttggcgg catagcgagg aaagaccgcg cagaaaacca
      721 ggccggtcaa aagagcggca aaggcaaaga caaagtgcga gaacagattg ggtttcagaa
      781 actggtttgc cggcggagct tcaaatgtac ggttcagttt tccgccgagt acaatggaag
      841 gcgaaagcac cagctctttc ggtgccgtgt agttcaggtc gccgccgatc accgttccgg
      901 gcagaatgac aatctcctgc gccgcaatgg aaacattccc ggcaattttc ccgccaagtg
      961 tcgctttttt tgccacgatc cgtacgttgc cggcgatagc gccttcgctg ataacgttgt
     1021 cgccggcacc gaacagattt ttggcaatcg atgccgatga ttcaattttg atggtattgc
     1081 ccgctgcggc cagcgagttg ccgaatgttc cggaagcctg caccatgcgc gaaaccagcc
     1141 gtacatgatc gccaaagcga cctgctgcaa tcacctgatt gccgcctccc gcccagacat
     1201 cgcctttaaa gtttccgcgc agatcaagcg tcgtacccgc cgcaaaaaga tcatccagcg
     1261 cttcgccgct gatggtgatg tcctgtgccg agatccacat ttcatcgcgc agcgtttcgg
     1321 attcgccgct gataaactga tcccgctgca caaactcaat tgcttgcact gtgcttccga
     1381 ccagcagcag cccggcgatc aaacccagtt tcaaatttct aatttcaggt ttcaagtttc
     1441 actttcctta cgtaaatccg gttgccttcc accttgatga tctcaatggc ggagttctgt
     1501 tcaatccatg ttccgtcggc gatgacgtcc gcgcggcggc cttcgatcag cgctttgccg
     1561 gctggacgca ggatgctcag cgctgtgcct ctcatgccgg ctttccatgt agagtcatcc
     1621 tgatctcttt tactgatatg ctgcgtcagc gaaagcgcct tgcccatccg gctcttcgga
     1681 aaatatttca tccagacaaa cgcggcgatt gcggtcagca caaggatgaa aaacaggctc
     1741 agccagccga gccacagcgg gaaaatcata aagcccatta ctgccgcgcc gatcaaggcg
     1801 gctgcaccgg ccgcgccgac gataccgccg gggatgaaaa tttccacccc gatcagaaac
     1861 agcccgcaaa ccagaagagt aatgtaccag ctcatgcgcg gcattctacc tgccttccgc
     1921 cgcgatggaa cacaaaagtt ccaattatcg gccggcctgc cggccgcagc tccgcaggag
     1981 cgaaggctgg taccccgggc gggatttgaa cccgcgacct acggtttagg aaaccgttgc
     2041 tctgtccagc tgagctaccg gagcgtgaaa agaaggcgga aatatgaagg atgaaacctg
     2101 aaaggggaat tcaaatttca agtttcagat ttcaagtttc cttccgg
//
LOCUS       DAOPZO010000202         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_77553_length_2111_cov_9.547179, whole genome shotgun sequence.
ACCESSION   DAOPZO010000202 DAOPZO010000000
VERSION     DAOPZO010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77553_length_2111_cov_9.547179"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PKI68_09155"
     CDS             <1..221
                     /locus_tag="PKI68_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53897.1"
                     /translation="PPVMRGKREFNDQSFYESLSTQFAGKGALSEKQVAALKKMIARY
                     AAQIPGYDTLREQYGLPEPKVKKAAASE"
     gene            218..1171
                     /locus_tag="PKI68_09160"
     CDS             218..1171
                     /locus_tag="PKI68_09160"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNX53898.1"
                     /translation="MNALADPCVEHFVNYLRGEKNASAHTVSNYLIDIRQFIELIWGE
                     EAKPPYNWKEADRFTARKFLVFFQKLEMEPATTGRKLSALRSFYKFLVREEYVPLNPF
                     SGLHLPKKGSYLPAVLSVNEIGQLLAAPQAMAAAAKPEGLFQEYMPVRDTAILEVLYS
                     TGMRIGELVSLTESRIDILSGIVSVLGKGKKERLCPLGRPAERALQNALAWREGIWQS
                     LGMKGDPRRTLFLNKHGGPLTARSIERMLKTYVGYCGLKPTISPHALRHSFATHLLDN
                     GADLRSVQELLGHASLSTTQIYTHVSVEKLKRVYEEAHPRA"
     gene            1258..>2111
                     /locus_tag="PKI68_09165"
     CDS             1258..>2111
                     /locus_tag="PKI68_09165"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNX53899.1"
                     /translation="MPMIWIIYNLLFPIGFLFMLPKFLTRMARRGGYRRHFEQRFGIY
                     GENTAARLAEARHIWIHAVSVGELFVALRFIEEYRKRNPDARFVLSTTTSTGHTLAEQ
                     KLDARDVLIYFPLDLPLVMNRVFNAINPQKLILVECEFWPNLVRQAHKRGIPVSLING
                     RISDSSFKGYMRLRPFTRRLLEMMDRVCMQGRQDAERILAMGARPATVKTLGTAKYDL
                     PPPAADADAKARAVLQTISVPDSALILTAGSTWPGEEEALCRIYKTLRAKHPELFLVL
                     VPRHAERR"
BASE COUNT          428 a          609 c          643 g          431 t
ORIGIN      
        1 atccgccggt catgcgcggc aagcgcgagt tcaacgacca gagcttttat gaatcgcttt
       61 caacccagtt tgccggtaaa ggcgcgctgt ccgaaaaaca ggttgccgcg ctgaagaaaa
      121 tgatcgcccg ttatgccgcc cagattccgg gctatgacac cctccgcgaa cagtacggcc
      181 tgccggaacc gaaagtgaaa aaagctgctg catccgaatg aacgcactgg cagatccttg
      241 tgtagagcat ttcgtcaact atctgcgggg ggagaaaaac gcttcggcgc atacggtcag
      301 caactacctg attgatatcc ggcagtttat tgagctgatc tggggcgaag aggcgaagcc
      361 gccgtacaac tggaaagagg ccgaccggtt taccgcccgc aagtttctgg tcttttttca
      421 aaagctggaa atggaaccgg cgacgaccgg gcgcaagctg tcggcgctgc ggtcgtttta
      481 taaatttctg gtgcgcgagg agtatgttcc gctcaacccg ttcagcgggc tgcacctgcc
      541 gaagaaaggc agttatctgc cggcggttct ctccgtgaat gaaatcgggc agctgctggc
      601 ggcgccgcag gcgatggcgg ccgcagccaa gccggaaggg ctgtttcagg aatatatgcc
      661 ggttcgcgat accgcgattc tggaggtgct ttacagtacc ggcatgcgta tcggcgagtt
      721 ggtcagtctg accgaatccc ggattgatat tctctccggc attgtcagcg tgctgggtaa
      781 agggaaaaag gagcggctct gtccgctggg ccggcccgcg gagcgcgcgc tgcagaatgc
      841 gctggcgtgg cgggagggca tctggcagtc gctcggcatg aaaggcgatc cgcgccgcac
      901 tctttttctg aacaagcacg gcggcccgct cacagcccgt tcgattgagc gaatgctgaa
      961 aacctatgtc ggctactgcg ggctgaagcc gacgatttct ccgcatgcgc tgcgccacag
     1021 ttttgccacg cacctgctgg ataacggcgc cgatctgcgc agcgtgcagg aactgctcgg
     1081 tcacgccagt ctctcaacaa cgcagattta cacccacgtt tcggttgaga aactgaagcg
     1141 cgtttatgaa gaagcgcacc cgagggcgtg agcatttagg atttatgatt gcagattgaa
     1201 ctggccagaa gggcgttgaa tatggacatt atgacggttt taaatctgaa atcttaaatg
     1261 cccatgatct ggattatcta caacctgctt ttcccaatag gctttctgtt catgctgccg
     1321 aagttcctga cgcgcatggc gcggcgcggc ggctaccggc ggcacttcga gcagcggttc
     1381 ggcatttacg gcgaaaacac cgccgcccgg ctggccgaag cgcggcatat ttggattcat
     1441 gcggtcagcg tcggcgagct gttcgtcgcc ttgcgtttta ttgaggaata ccgcaagcgc
     1501 aatccggatg cgcgctttgt gctgagcacc accacctcga ccggccacac gctggccgaa
     1561 cagaagctcg atgctcgcga cgtgctgatc tatttcccgc tcgatctgcc gctggtcatg
     1621 aaccgcgtgt tcaacgccat caacccgcag aagctgattc tggtcgaatg cgagttctgg
     1681 ccgaatctgg tgcggcaggc gcataagcgc ggcattcccg tgtcgctgat caacgggcgc
     1741 atttccgatt cctcgttcaa aggctatatg cggctgcgcc cgttcacgcg ccggctgctc
     1801 gaaatgatgg atcgggtctg catgcagggc cggcaggacg cggaacgtat tctggcgatg
     1861 ggcgcgcgcc cggcaacggt caagacgctc ggcaccgcca agtatgacct gccgccgccc
     1921 gccgcggatg cggatgccaa ggcccgcgcg gtgctccaaa cgatttccgt tccggacagc
     1981 gcgctgattc tgacggccgg ttcgacctgg ccgggcgaag aggaagcgct ctgccggatc
     2041 tataaaacgc tgcgcgccaa gcacccggaa ctctttctgg tgctggtgcc gcgccacgcc
     2101 gagcgccgcg a
//
LOCUS       DAOPZO010000203         2104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_77962_length_2104_cov_11.811127, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000203 DAOPZO010000000
VERSION     DAOPZO010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77962_length_2104_cov_11.811127"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PKI68_09170"
     CDS             complement(<1..923)
                     /locus_tag="PKI68_09170"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX53900.1"
                     /translation="MIIGGRIRTKAPYYTKKNSRRLRPAPFFPITTAVNQPFSEQLNR
                     CECCPRRCGVDRLAGQAGFCRVGGEILLSHAGLHYGEEPPISGTRGSGTLFFAGCNLR
                     CVFCQNWQISQEFDFRWNATSGRVQSVLGEPAPPKALSPAELADEMLRLQADGAHNIN
                     FVSPSHMIFQMADAILAARQKGLTVPIVYNSNGYDSVAALRQIRGLVDIYLPDIKYMD
                     NALGRKFSAADNYADVIPGVLREMRDQVGPLELDDDEIAVRGLLVRHLVLPGQIENSK
                     ASLKVLAELNPEITLSIMSQYSPQYKACGIP"
     gene            916..1644
                     /gene="hisA"
                     /locus_tag="PKI68_09175"
     CDS             916..1644
                     /gene="hisA"
                     /locus_tag="PKI68_09175"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNX53901.1"
                     /translation="MITIIPAIDLKGGKCVRLRQGLANEETVYSDSPVEMAKHWVRAG
                     GEFLHVVDLDGAFMGHPVHTEVLREICAAIKIPVEIGGGIRTDEHIEQLLATGVCRVI
                     LGTRACEHPEELARLVQKFGGERIAVGIDARAGKVQTKGWVETTDIEAVDLAKKVDAA
                     GVGTIIYTDTARDGMLDGVNAFEMGKICSAVACNVVASGGVSSIEDIQRLAALNCDNL
                     TGAIVGKALYEKTLTVRRLKKAVQ"
     gene            1782..>2104
                     /locus_tag="PKI68_09180"
     CDS             1782..>2104
                     /locus_tag="PKI68_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNX53902.1"
                     /translation="MARTVPMSKVRNIGIMAHIDAGKTTFSERILFYSGKSHKIGEVH
                     DGAAVMDWMVQEQERGITITSAATCTMWKDHMISLIDTPGHVDFTMEVERSLRVLDGA
                     IALFCA"
BASE COUNT          447 a          558 c          654 g          445 t
ORIGIN      
        1 tccggaattc cgcaggcttt atactgcggt gaatattgcg acatgatgct gagggtgatt
       61 tccgggttca gttccgccag aactttgagg ctggctttgc tgttttcaat ctgtcccgga
      121 agaaccaagt ggcggacgag cagtccgcgc acggcgattt cgtcgtcgtc cagctccagc
      181 ggtccgacct gatcacgcat ttcgcgcaga acgccgggaa tgacatcggc ataattgtct
      241 gccgccgaaa atttccggcc gagcgcatta tccatatatt taatgtcggg caggtaaata
      301 tcgaccagtc cgcgaatctg ccggagcgcg gcgacagagt cgtagccgtt ggagttgtag
      361 acgattggaa cggtaagtcc tttttgccga gcggccagaa tggcgtcggc catctggaaa
      421 atcatgtgcg acggcgagac gaagttgatg ttgtgcgcgc cgtccgcctg caaccggagc
      481 atttcgtcgg cgagttcggc agggcttagt gcttttggtg gagcgggctc tccgagcacg
      541 ctttgaacgc gcccggaggt cgcgttccac ctaaaatcaa attcctgact gatctgccag
      601 ttctggcaga agacgcaacg cagattgcat ccggcgaaaa agagggttcc ggagccgcgc
      661 gttccggaga tcggcggctc ttcgccatag tgcagtccgg cgtgcgaaag caggatttct
      721 ccgccgacgc ggcagaatcc ggcttggccc gccagccggt cgacgccgca acggcgcgga
      781 cagcattcgc accggttcaa ctgttctgaa aagggttggt tcacggcggt ggttatagga
      841 aagaacggcg ccgggcgcaa ccggcggctg ttttttttcg tgtaatacgg ggctttggtg
      901 cgtattctcc cgccgatgat cacaattatt ccggcaattg atttgaaggg cggcaagtgc
      961 gtgcgcctgc ggcagggtct ggcgaatgag gagacggttt attccgattc tccggtcgaa
     1021 atggccaaac actgggtgcg ggcgggcggc gaatttctgc acgtggtgga tctcgacgga
     1081 gcgttcatgg gccatccggt tcatacggaa gtgctgcgcg aaatctgtgc cgcgattaag
     1141 attccggtcg aaatcggcgg cggtatccgc accgatgaac atattgaaca gctgctggca
     1201 accggcgtct gccgcgtcat tctcggcacc cgcgcctgcg agcacccgga agagctggcc
     1261 cggctggttc aaaagttcgg cggcgagcgg atcgcggtcg gcattgatgc ccgcgcgggc
     1321 aaagtccaga ccaagggctg ggtggaaacg acggatatcg aagcggtcga tctggcgaaa
     1381 aaggtggatg cggccggcgt cggcaccatt atttatacgg atacagcccg cgacgggatg
     1441 ctcgacggcg taaacgcctt tgaaatgggc aaaatctgct cggcggttgc ctgtaatgtg
     1501 gtcgcttcgg gcggcgtttc gagcattgaa gatatccagc ggctggcggc gcttaattgc
     1561 gataatctga ccggcgcgat tgtcggcaaa gccctttacg aaaaaacgct tactgtccgg
     1621 cggctgaaaa aagcggtgca gtagtttctt tcgcgcagtt atacggagcg gcgggggctt
     1681 aacttttaac gtgtttgcgc ttgaaggcgc gggcccgaaa cgccatattg cgcggctctt
     1741 ttatttaagg aatagaacag aaccaaagcg aggatagaac catggccaga acagtaccaa
     1801 tgtccaaagt ccgtaatatc ggaattatgg cgcacatcga cgccggtaaa accaccttca
     1861 gcgaacgcat tctcttttac tccggcaaat cgcacaaaat cggcgaagtg catgacggcg
     1921 cggcggttat ggactggatg gttcaggaac aggaacgcgg tatcaccatt acttccgccg
     1981 caacctgcac gatgtggaaa gaccacatga tttcgctgat cgacaccccc ggccacgtgg
     2041 acttcaccat ggaagtggag cgctccctgc gcgtactcga cggcgcgatc gcgctgttct
     2101 gcgc
//
LOCUS       DAOPZO010000204         2080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_79322_length_2080_cov_10.200000, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000204 DAOPZO010000000
VERSION     DAOPZO010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79322_length_2080_cov_10.200000"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1743
                     /locus_tag="PKI68_09185"
     CDS             139..1743
                     /locus_tag="PKI68_09185"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNX53903.1"
                     /translation="MKRLNWLHVLAPLAVSGSCATAFAAPTPPAKPNIIIVLADDLGY
                     GDVSCYNSESKIQTPFLDRMAAEGMRFTDAHTCSSVCTPSRYGLLTGRYSWRSRLKHG
                     VLYGYSTALLKPPRETIASLLKKNGYRTACIGKWHLGMDMATTDGKPTDQSETTPSNI
                     DWNGAIRNGPNAAGFDYYFGCAASWDMPPYVWIENDKFTTTQFAEAAKTEFGRPGIKA
                     VGLAATNGLPTLTQKVVSYIETSAKEKSGKPFFLYFPLTAPHAPIAPNAVFKGRSQAG
                     DYGDFVTETDDTIGQLLAALARTGLDSNTLVIVTSDNGPEVFAIARKTEYNHFSAGKL
                     LGIKRDNWDGGHRVPFIARWPGQVPAGSVSGETICLTDLMATAAALAGCELPPNAGED
                     SYNIWPVLAGEKPAAPIREATVHHSFKGFFAIRQGQWKLLLHTGSGGNRYEGQPAYAA
                     YYSKPVQLYDMNADPYETVNLADRHPEIVAQLTALLKQYVLNGRSTPGPAVPNDTPNN
                     WSQLNWMNRTAEKPAKASGAKPVSAE"
     gene            1901..>2080
                     /locus_tag="PKI68_09190"
     CDS             1901..>2080
                     /locus_tag="PKI68_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53904.1"
                     /translation="MKRLNWLHVLAPLAVSGSCATAFAAPTPPAKPNIIIVLADDLGY
                     GDVSCYNSESKIQTPF"
BASE COUNT          470 a          723 c          549 g          338 t
ORIGIN      
        1 tggatgaacc gcaccgctga aaaaccggca aaagccagcg gcgcaaaacc agtctatgcc
       61 gaataacttg gcaaatttac gtttagtgtg cgcacgtttc gtgctttgtg gccctaacct
      121 gaaggaagga tcgatccgat gaaacgactg aactggctcc atgttctggc tccgctcgct
      181 gtcagcggca gctgcgcaac cgccttcgcc gccccgacgc cgcccgccaa acccaatatt
      241 atcatcgtcc tcgccgacga cctgggctac ggcgacgtca gctgctacaa cagcgaatcg
      301 aaaatccaga cgccgttcct cgaccggatg gccgcggaag gcatgcgctt caccgacgcc
      361 cacacctgca gctccgtctg cacgcccagc cgctacgggc tgctgaccgg acgctacagc
      421 tggcgctcgc gactgaaaca cggcgttctc tacggctact ccacggccct cctcaagccg
      481 ccccgcgaaa cgatcgcctc cctgctgaag aaaaacggct accgcacggc ctgtatcggc
      541 aaatggcacc tggggatgga tatggccacc acggacggca agccgaccga tcagtccgaa
      601 acaacccctt ccaacatcga ctggaacggc gccatccgca acggacccaa cgccgccgga
      661 ttcgattatt acttcggctg cgccgcctcc tgggacatgc cgccctacgt ctggatcgaa
      721 aacgacaaat tcaccaccac ccagttcgcc gaagcagcca aaaccgaatt cggccggccc
      781 ggaatcaagg cggtcggcct ggccgcgacc aacggcctgc cgacactcac ccagaaagtg
      841 gtctcctata ttgagacctc cgcgaaagaa aagagcggca aaccgttctt cctctatttc
      901 ccgctgacgg caccccatgc gccgatcgca cccaacgcgg tctttaaagg ccgcagccag
      961 gccggcgact acggcgactt tgtaaccgaa accgacgaca cgatcgggca gcttttggcg
     1021 gcgctcgccc ggaccggcct cgattccaac actctggtca tcgtcaccag cgacaacggc
     1081 cccgaagtgt tcgccatcgc ccgcaaaacg gaatacaacc acttcagcgc gggaaaactg
     1141 ctcggcatca aacgcgacaa ctgggacggc ggccatcgcg ttccgttcat cgcccgctgg
     1201 cccggacagg ttccggccgg cagcgtcagc ggagaaacca tctgcctgac cgacctgatg
     1261 gccacggccg ccgcgctggc cggctgtgag ctgccgccga acgccggtga agacagctat
     1321 aacatctggc ccgtactggc cggcgaaaaa ccggccgcgc cgatccgcga agccacggtg
     1381 caccactcct tcaagggttt cttcgccatt cgtcaaggac agtggaaact gctgctgcat
     1441 accggttcgg gcggcaacag atatgaaggc cagccggcct atgcggccta ctacagcaaa
     1501 ccggttcagc tctacgacat gaacgccgac ccgtacgaaa cggtcaacct ggccgaccgc
     1561 cacccggaga ttgtcgcgca actgaccgcg ctgctgaaac agtatgtgct caacgggcgc
     1621 agcacccccg gcccggccgt accgaacgat acgcccaaca actggagcca gctcaactgg
     1681 atgaaccgca ccgctgaaaa accggcaaaa gccagcggcg caaaaccagt ctctgccgaa
     1741 taactgggcg gccaagagcg cggtgcaaac cgcgctcttg gactgtaagg cacccccgga
     1801 aaatgatttt tctgttttcc gtgcattaca aaccgcggat ttacgtttag tgtgcgcacg
     1861 tttcgtgctt tgtggcccta atcaaggaag gatcgatccg atgaaacgac tgaactggct
     1921 ccatgttctg gctccgctcg ctgtcagcgg cagctgcgca accgccttcg ccgccccgac
     1981 gccgcccgcc aaacccaata ttatcatcgt cctcgccgac gacctgggct acggcgacgt
     2041 cagctgctac aacagcgaat cgaaaatcca gacgccgttc
//
LOCUS       DAOPZO010000205         2068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_80047_length_2068_cov_9.192747, whole genome shotgun sequence.
ACCESSION   DAOPZO010000205 DAOPZO010000000
VERSION     DAOPZO010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80047_length_2068_cov_9.192747"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="PKI68_09195"
     CDS             complement(<1..824)
                     /locus_tag="PKI68_09195"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNX53905.1"
                     /translation="MNIDPSTRQKFLTFQRNELTEYHIYRELAKVTKNSANRDVLNRI
                     ANDELRHYEQLKKATGQEVSRDRFKIWKYVLIARLLGLTFGIKLMEAGEANAQTGYDD
                     FEDEFEPAGLMSADENEHEQELIGLLDEERLQYTGSVVLGLNDALVELTGALAGFTLA
                     LQNTKLIAMTGAITGIAAALSMATSEYLSTKAEETEKHPLKASVYTGIAYIFTVLVLI
                     APYLIFQNYYVCLGITLILGIGIIASFNYYIAVAKDLNFRKRFLEMSCLSLGVAAI"
     gene            complement(937..1476)
                     /locus_tag="PKI68_09200"
     CDS             complement(937..1476)
                     /locus_tag="PKI68_09200"
                     /inference="COORDINATES: protein motif:HMM:NF016885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HNX53906.1"
                     /translation="MKISYRKSAFVLLFPLLASLLAGCAWQKTLKDRVAVYGHRNWIV
                     VADSAYPKQSNPGIETVQTGSSQIEVLKAVLKEIEGAPHVQAVVMLDSELDSVTEADA
                     PGVEAYRAELKKLLAHKQVKVMQHEKIIRELDEGAKLFNVLLLKSDMTIPYTSVFIQL
                     DCGYWDAEKEARLRKALKK"
     gene            complement(1561..>2068)
                     /locus_tag="PKI68_09205"
     CDS             complement(1561..>2068)
                     /locus_tag="PKI68_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53907.1"
                     /translation="KTKTAAKKPAGGSAWKKTLKERVPVYGHRNWIVVADWAYPQQSN
                     PGIETIYTGGKQLDVLKTVLKEIEDAPHIQAIVMLDAELDSVTEADAPGVEAYRKGLK
                     KLLEHKQVKVMQHEKIIHELDEGAKLFNVLLLKSDMTIPYTSVFLQLDCGYWNAEKEA
                     RLRKAFKG"
BASE COUNT          374 a          579 c          585 g          530 t
ORIGIN      
        1 ctgatggccg ctacgccgag gctcaggcag ctcatctcca gaaaccgttt gcggaagttc
       61 agatctttgg caacggcgat gtaatagttg aatgaagcaa taatcccgat gccgaggatc
      121 agggtgatgc ccaggcagac gtagtaattc tggaaaatca gataaggcgc aatcagcacc
      181 agcaccgtga aaatataagc aatgccggta taaaccgagg ctttcagcgg atgcttctcc
      241 gtctcttccg ccttggtcga aagatattcc gacgtggcca tggagagcgc cgccgcgatg
      301 ccggtgatgg cgccggtcat ggcaatcagt ttggtgttct gcagcgccag cgtaaagccg
      361 gccagcgcgc cggtcagttc caccagcgcg tcgttgaggc cgagcacgac cgagccggta
      421 tattgcagcc gttcttcgtc cagcaggccg atcagctcct gttcgtgttc gttttcgtcg
      481 gcggacatca ggcccgccgg ttcaaactcg tcttcgaaat cgtcataacc ggtttgggcg
      541 ttggcttctc cggcttccat cagtttgatg ccgaaggtca gccccagcag ccgggcaatc
      601 agtacatact tccagatttt gaaccggtcg cgggaaacct cctgcccggt ggccttcttg
      661 agctgttcat agtgccgcag ctcatcgttg gcgatgcggt tgagtacgtc gcggttcgcg
      721 ctgtttttcg tcaccttcgc cagctcgcgg taaatgtggt attccgtgag ctcgttgcgc
      781 tgaaaagtca gaaacttctg ccgggtggat ggatcgatat tcataggccc ggactatatc
      841 agcttccggc cggttgaaaa tgtttcttcc ggcacaaaaa aaatcccggc gggccgcaag
      901 gcccgtcggg aggtccgcaa tctggtgaag cgccgattat tttttgagcg cttttctcag
      961 gcgggcttct ttttccgcgt cccagtagcc gcagtccagc tggatgaaga cagaggtgta
     1021 ggggatggtc atgtcgcttt tcagcagcag gacattgaag agtttggccc cttcgtccag
     1081 ctcgcgaatg atcttctcgt gctgcatcac tttgacctgc ttgtgcgcga gcagcttttt
     1141 gagttctgcg cggtaggctt caacgcccgg ggcatccgct tcggttacgc tgtcgagttc
     1201 ggagtccagc atcacgacgg cctggacatg cggcgcgcct tcgatttctt tcaggaccgc
     1261 cttgagcacc tcgatctgcg agctgccggt ctgtacggtc tcaatgccgg ggttgctctg
     1321 tttgggatag gcgctgtctg ccaccacaat ccagttgcga tggccgtaga ccgcgacccg
     1381 gtctttcagt gttttctgcc aggcgcagcc ggccagcagc gaggccagca gcgggaacaa
     1441 caaaacgaat gccgattttc tgtaacttat tttcattctg tacctttctt ctgtgtttac
     1501 cttcacccgg ctcgctgttg atttttcaca acaggcgagc gcttatgaaa ctctgcgggc
     1561 ttaacccttg aatgctttgc gcagacgggc ttctttttcg gcgttccagt atccgcagtc
     1621 cagctgaagg aatacggatg tgtaaggaat cgtcatatcg ctcttcagca gcagaacatt
     1681 gaacagcttc gcgccttcgt ccagctcgtg gatgatcttt tcgtgctgca tcactttgac
     1741 ctgtttgtgt tcgagcagct tcttgaggcc tttgcggtag gcttcaacgc cgggggcatc
     1801 cgcttcggtc acgctgtcca gctctgcgtc cagcatcacg atcgcctgaa tgtgcggggc
     1861 gtcttcaatt tccttcagca cggtcttgag cacgtccagc tgcttgccgc cggtataaat
     1921 ggtttcaatg ccggggttgc tctgctgcgg ataggcccag tcggccacga cgatccagtt
     1981 gcggtgtccg tagaccggca cccgttcctt gagcgttttc ttccatgcgc tgccgccggc
     2041 cggttttttg gctgcggttt tggtctta
//
LOCUS       DAOPZO010000206         2037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_81903_length_2037_cov_5.136731, whole genome shotgun sequence.
ACCESSION   DAOPZO010000206 DAOPZO010000000
VERSION     DAOPZO010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81903_length_2037_cov_5.136731"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..917
                     /locus_tag="PKI68_09210"
     CDS             165..917
                     /locus_tag="PKI68_09210"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNX53908.1"
                     /translation="MSRFQIFFSLLLIAAFPLFAEVELNGNPQELTAYLSTIPETVMI
                     EGKAEKKLSADRAIIQLKICTEGKSMDASLAANRRLREEITVKLLAAGLTTNGIQAAK
                     FSSTPESGFFSDKVRSYKVQNTMKVTVVNEAEFRAVAALIDAHKEISYADTEFELSTK
                     KEVEKALLAEACQDATAKKAIYEAGLNVALTPVRFHNGAIQMQRPMMARTAKMASYVM
                     EDVALGAAAPPIQFDEMVFNASITVEYRLQAK"
     gene            956..1354
                     /locus_tag="PKI68_09215"
     CDS             956..1354
                     /locus_tag="PKI68_09215"
                     /inference="COORDINATES: protein motif:HMM:NF023374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3465 domain-containing protein"
                     /protein_id="HNX53909.1"
                     /translation="MKKALFVIIILLTTCFTGFSGGADAVVASAFANHKSSIQVQGCG
                     QVIKVLSDDNNGSRHQRFIIKLTSGQTLLIAHNIDLAKRVAAIQTGDTVAFSGEYEWN
                     SQGGVIHWTHRDPEGKHAAGWLKHKGQVYQ"
     gene            1351..1593
                     /locus_tag="PKI68_09220"
     CDS             1351..1593
                     /locus_tag="PKI68_09220"
                     /inference="COORDINATES: protein motif:HMM:NF021429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HNX53910.1"
                     /translation="MTSPTEHPRDPLHGITLEKILIYLVEEYGWEEMGGRIRIRCFQS
                     DPSIKSSLTFLRKTPWAREKVEKWYVADVRRKQRRR"
     gene            1704..>2037
                     /locus_tag="PKI68_09225"
     CDS             1704..>2037
                     /locus_tag="PKI68_09225"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNX53911.1"
                     /translation="MNLDVLYHDNQMLAVNKPAGLLTQPSGTDKDNLEDRAKAYVKEV
                     KGKPGNVFLHAVHRLDQVVSGVVLFACTDKALSRLNADMRAHRFTKIYHAVVSGMPPQ
                     QAGSLRHFL"
BASE COUNT          503 a          564 c          544 g          426 t
ORIGIN      
        1 actgcggcga aatgctcctc accggcaaac ccgacgttat gcccctgccg agcggggctt
       61 tcgcgaggtg gatggccgcc gaatagcgtg ccttccaaac agttgaaaga tttcttccaa
      121 tgtctggaaa actgcctatg ctttcccgca acaaggaggc ttttatgtca cgttttcaaa
      181 tatttttctc tcttctgctg atcgccgcat tcccgctgtt cgccgaggtt gaattgaacg
      241 gcaacccgca ggaactgacc gcttatctat ccaccattcc ggaaacggtc atgatcgaag
      301 gcaaggcgga aaagaaactg tccgccgacc gcgccattat tcagctgaag atctgtacgg
      361 aaggcaaatc gatggatgct tcgctcgccg caaaccgtcg tctgcgcgaa gaaatcaccg
      421 tcaaactgct ggcagccgga ctgacgacca acgggattca ggctgcgaaa ttttcctcca
      481 caccggaaag cggcttcttc agcgacaaag tccgcagcta caaggtgcaa aacaccatga
      541 aagtcaccgt cgtcaacgaa gccgagttcc gggcggttgc cgcgctgatc gacgctcata
      601 aagaaatcag ctacgccgat accgaattcg aactctccac caagaaagag gtcgaaaagg
      661 cgctactcgc cgaagcctgt caggatgcca cagccaaaaa agcaatttat gaagcgggcc
      721 ttaacgtcgc gctgaccccc gtccgttttc ataacggagc aatccagatg cagcggccca
      781 tgatggctcg cacggcaaaa atggccagct acgtaatgga agatgttgct ctgggcgcag
      841 cagccccgcc gattcagttt gatgaaatgg ttttcaacgc cagcattacc gtcgaatacc
      901 ggcttcaggc aaaatgatcc tcaccggcaa ggtcggcgca atggatctgc aataaatgaa
      961 aaaggctcta tttgtcataa ttatcctgct cacgacctgc tttacggggt ttagcggtgg
     1021 agccgatgcg gttgtggcgt cggcttttgc caatcacaaa agcagtattc aggtgcaggg
     1081 ttgcggacag gtcataaagg ttctgtcgga tgataacaac ggaagccgtc accagcgctt
     1141 cattatcaag ctgacctcag gacaaacact tttgatcgcg cataacatcg atttggcaaa
     1201 acgggttgcc gccatacaaa ctggcgacac tgttgcgttt tccggtgaat acgaatggaa
     1261 ttcacaaggc ggtgtaatcc attggactca ccgtgatcct gaaggaaagc acgcagccgg
     1321 atggttaaag cacaaaggac aagtttatca atgacctcgc caactgaaca tccgcgcgac
     1381 ccgctgcacg gcattacgct ggaaaaaatt ctcatttatc tggtcgagga gtacggctgg
     1441 gaggagatgg gcgggcgcat tcggatccgg tgctttcaat ccgatccgtc gatcaaatcg
     1501 agtctgacat ttctgcgcaa gacgccgtgg gcgcgggaga aggtggagaa gtggtatgtc
     1561 gccgatgtcc gccggaaaca gcgccgccga taattgccca cgaatcttca tacatagaac
     1621 cctctatatc ccattcgcgc tgattcgtgg gtttcgtggg aaaaacttgg cacgctgggt
     1681 cttctgcatc atgattcgcg cccatgaatc tcgacgtgct gtatcacgac aatcaaatgc
     1741 tggcggtgaa taagccggcc gggctgctga cgcaaccgag cggcacggac aaagacaatc
     1801 tcgaagaccg cgccaaggcc tacgtcaagg aggtcaaagg caagccgggc aatgtgttcc
     1861 tgcacgcggt tcaccggctc gaccaggtcg tcagcggcgt ggtgctcttt gcctgcaccg
     1921 acaaagcgct gtcgcggctg aacgccgaca tgcgcgcgca ccgcttcacg aaaatttatc
     1981 acgcggtcgt ctccggtatg ccgccgcagc aggccggttc gctgcgccat tttctga
//
LOCUS       DAOPZO010000207         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_90168_length_1914_cov_9.006455, whole genome shotgun sequence.
ACCESSION   DAOPZO010000207 DAOPZO010000000
VERSION     DAOPZO010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90168_length_1914_cov_9.006455"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..669)
                     /locus_tag="PKI68_09230"
     CDS             complement(268..669)
                     /locus_tag="PKI68_09230"
                     /inference="COORDINATES: protein motif:HMM:NF017482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HNX53912.1"
                     /translation="MNERTTEPDVWSYALPGGWEALAGKTDADNDILSLRVARANDLW
                     FHVHGLPGSHVVLRGPEGETASNELIKSAAAIAAWHSKARSAGVVPVSCTEAKNVSKP
                     RGAKPGSVCIKREKTIKVRPGLPGKESQIRS"
     gene            complement(797..>1914)
                     /locus_tag="PKI68_09235"
     CDS             complement(797..>1914)
                     /locus_tag="PKI68_09235"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HNX53913.1"
                     /translation="AEQTAQVENANAAKIAQIEQDNAKKTDSLEKLQAEAKKNEASSW
                     ANNIKLAGDIRARYEKRDVADTTTKDRQRIRLRVGAYGKVNDQVDFGIRLATGGTRTS
                     SNQDMGDNAGMKNIYLDQAFIDYHPEQVEGLHTFVGKMPQPWITYNTGLIWDTDLNPE
                     GAAVTYEKQFVPFKLKANAGSFIFQDNLEDDIRLWSGQVAVEKKIGDTTLTLGVSDYA
                     FQNASGATNLTGALNAKSANTPNGDFNLVEGFGAYEFKVLKMPITLNGQYVVNTLAAS
                     SDDKAYLLGVTVGKAKDKGTWELGYSWRDIGNDAVVGGLNDSDFAGETWTGCYGHRFN
                     AKYMVLKNMSADLAYILSTDYKNREANTLQADLNFKF"
BASE COUNT          398 a          549 c          504 g          463 t
ORIGIN      
        1 cgcattccaa attaccgcct caaggaatgc tgcgaagcga tccctgcgct gcaagccaag
       61 ccgcccctca cgatccggaa aagtctctca gctctccggc tgaaagtgtg ggacgaagag
      121 cgcaaacagc tcaccggctt ccgctaagac atcaagtata aaacaagcag ccctgttaaa
      181 cttcaggtcg tcataaaacc gcagaaggaa agccatgaat cggccggcaa agccgccttc
      241 ggctcggccg attcggccaa ttcgcctcta tgagcgaatt tgactctctt tgcccggcag
      301 cccgggacga accttgatgg ttttttcgcg cttaatgcag accgatccgg gcttcgcgcc
      361 gcgcggtttg gaaacattct ttgcttccgt gcaggagacc ggaacaacgc cggcactgcg
      421 tgctttgctg tgccacgcgg caatcgcagc ggcgctttta atcagctcgt tcgacgccgt
      481 ttcgccctcc ggaccgcgca gaacaacgtg actgccgggc agcccgtgaa cgtgaaacca
      541 gagatcgttt gcgcgcgcga ccctcaggct gagtatgtcg ttgtcggcat cggtttttcc
      601 ggccagcgcc tcccagcctc ccggcagcgc atagctccag acatcgggtt ctgtcgttct
      661 ttcattcatc gacgggaacc taccagaaag tgaacgcact gccatgggaa gatttcagaa
      721 aaggtgcggg ctcccggaaa ccgggagccc gcgggatttg atctgctcac gcagatcagg
      781 cagtcgaaca ggagacttaa aacttgaagt tcagatcggc ctgcagggtg tttgcttccc
      841 tgtttttgta atcggtgctg aggatgtaag ccagatcggc ggacatattt ttcaacacca
      901 tgtatttcgc attgaaccgg tggccgtaac agccggtcca ggtttcgccg gcaaagtcag
      961 agtcgttcag gccgccgaca acggcatcgt taccgatgtc gcgccagctg taaccgagtt
     1021 cccaggtccc tttgtctttg gctttgccga cggttacccc gagcaggtaa gccttgtcgt
     1081 cactgctggc agccagggta ttaaccacat actgaccgtt cagagttatc ggcatcttga
     1141 gaactttgaa ttcgtatgca ccgaatcctt cgacaagatt gaaatcgccg ttcggggtat
     1201 tggccgactt ggcattcaat gcgccggtca ggttggtcgc cccggatgca ttctggaagg
     1261 catagtcact cacaccaagt gtcagcgttg tatcaccgat ctttttctca accgccacct
     1321 gtccgctcca gagccggatg tcgtcttcga ggttgtcctg aaaaatgaac gaaccggcat
     1381 tggctttcag cttgaagggg acgaactgtt tttcatacgt cactgcggcg ccttccgggt
     1441 tcagatcagt atcccagatc agtccggtat tataagttat ccagggctgc ggcatttttc
     1501 cgacgaacgt atgcagcccc tcaacctgct ccgggtggta atcaataaat gcctgatcca
     1561 gataaatatt cttcatgccg gcgttgtcgc ccatatcctg attggaagaa gtgcgtgttc
     1621 cgccggtggc cagacggatg ccgaaatcaa cctgatcatt gacttttccg taagcaccga
     1681 cccgcagacg gatgcgctga cggtctttgg ttgttgtgtc ggccacatca cgcttctcat
     1741 aacgggcgcg gatatcgccg gccagtttaa tgttattcgc ccacgaagag gcctcgtttt
     1801 tcttcgcttc agcctgaagc ttctccagcg aatctgtttt tttggcattg tcttgctcga
     1861 tctgggcgat tttagccgca ttcgcgtttt cgacctgcgc ggtctgttcc gcca
//
LOCUS       DAOPZO010000208         1908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_90616_length_1908_cov_9.697787, whole genome shotgun sequence.
ACCESSION   DAOPZO010000208 DAOPZO010000000
VERSION     DAOPZO010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90616_length_1908_cov_9.697787"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="PKI68_09240"
     CDS             complement(<1..1118)
                     /locus_tag="PKI68_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNX53914.1"
                     /translation="MGVKAVGLILGGGAGTRLQPLTQDRAKPAVPIAGKYRLVDIPIS
                     NCINSGIRSIYLLTQFNSMSLHRHIQSTYIFDQFSPGYVRMLAAEQTPTSSGWFQGTA
                     DAVRQSMRHYMISEPDIVVILSGDQLYRMDFSKVIEEHLERGADVTICTKPVPRREAG
                     SLGIMHVDEKNKIVEFVEKPGDTPRLNDLRAPMYKEERYLASMGIYIFNTKVLTSLLC
                     GNDHMDFGKNIIPAAIHNCNVYSHIFEGYWKDIGTIGMFHDANLALTDLVPEFTFYDT
                     TAPIYTHMRYLPPSKINCCDLNRCLLAEGCIISGHRILRSVIGLRAVIGEGTVIEHSV
                     IMGADFFEHEKTADNSGNVRLGIGRDCYIKNAIIDKNVR"
     gene            complement(1207..>1908)
                     /locus_tag="PKI68_09245"
     CDS             complement(1207..>1908)
                     /locus_tag="PKI68_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNX53915.1"
                     /translation="SATLAVGEDLSWFRKRIGAEHCDELQVGSPFDYGRQMRVKIPAG
                     MPDPSEEQAFEEAVIEVLPRYIAQSRGRAFVLFTNAGFMRRVAERLKASIEDQGHDLL
                     VQGAGLPPQTMLKRFREHGSAVLFGLDRFWMGVDVRGDALSSVIIVRLPFAVPDHPLI
                     QARLEDVKARGGDAFRDYSLPSAVLKFRQGTGRLIRTASDEGTVVILDPRVTNKWYGR
                     LFLRALPECPVEIEE"
BASE COUNT          418 a          562 c          523 g          405 t
ORIGIN      
        1 cgtacgtttt tatcgatgat ggcgttcttg atgtagcagt cgcggccgat accgagccgg
       61 acgtttccgc tgttatccgc tgtcttttcg tgctcaaaga aatcggcgcc cataatgacg
      121 gaatgttcaa tcacagtgcc ttcgccaatc accgcgcgca gaccgatgac agagcgcaga
      181 atccggtgac cggaaataat gcatccctca gccagcagac agcggttcaa gtcgcagcag
      241 ttgattttgg acggcggcag atagcgcata tgcgtataga tcggcgcggt cgtgtcgtaa
      301 aaagtgaatt ccggaacgag atccgtcagc gccagattgg cgtcatgaaa cataccgatg
      361 gttccgatgt ctttccagta gccttcaaag atatggctgt agacgttgca gttgtgaatg
      421 gccgccggaa taatattttt cccgaagtcc atgtggtcgt tgccgcacag aagactggtc
      481 agaactttcg tgttgaaaat gtaaatcccc atgctggcaa ggtagcgctc ttccttatac
      541 atcggcgcac gcaggtcatt gagccgcggg gtgtcacccg gcttttcaac aaattcgacg
      601 attttgtttt tttcatccac atgcatgatg cccagcgagc ccgcttcccg gcgcggcacc
      661 ggcttggtgc agatggtgac atccgcgccc cgttccagat gctcttcgat cacttttgaa
      721 aaatccatgc ggtaaagctg atcgccggaa agaataacga cgatatccgg ctcactgatc
      781 atatagtgtc tcatcgactg gcggacggcg tcggccgtgc cctggaacca gccgctggag
      841 gtcggcgtct gctcggcggc gagcatccgg acatagcccg gcgagaactg atcgaaaatg
      901 taggtggact gaatgtgccg gtgcagcgac atgctgttga actgagtgag cagatagatg
      961 gaacggatgc cgctgttaat gcagttgctg atgggaatat cgaccagccg gtattttccg
     1021 gcaatcggaa ccgccggttt ggcgcgatcc tgtgtgagag gttgaagacg ggttccggcc
     1081 ccgccgccca gaatcagacc gaccgcttta acacccatat ccgcactcct tcccgaagct
     1141 gataaatgtg tactttatag tcgttacgtt tatttctccg ccagaacaaa atgatgaccg
     1201 gagcggttat tcttcaattt caaccggaca ttcaggcagc gcgcgcagaa aaagccgccc
     1261 gtaccactta ttggtcaccc gcggatcgag aatcaccacc gtgccctcgt cgctcgccgt
     1321 acggatcagc cgccccgttc cctgccggaa cttcagcacg gccgacggca gagaatagtc
     1381 acggaaggcg tcaccgccgc gcgctttcac atcctccagc cgggcctgaa tcagcggatg
     1441 atccggaaca gcaaacggca ggcggacaat gattacactg ctcagtgcat cgccgcggac
     1501 gtccacgccc atccagaaac gatccagccc gaacagtacg gccgagccgt gttcgcggaa
     1561 ccgtttcagc attgtctgcg gcggaagtcc ggcgccctgc accagtagat cgtgcccctg
     1621 atcttcaatg gacgctttca gccgttccgc aacgcggcgc ataaaacccg cgttggtgaa
     1681 aagtacgaat gcccgcccgc ggctctgcgc gatataccgc ggaagcactt caatcacggc
     1741 ctcctcgaat gcctgctcct ccgaaggatc cggcataccc gccggaattt tgacccgcat
     1801 ctggcggccg tagtcgaacg gcgaaccgac ctgcagttcg tcgcaatgct ctgcgccgat
     1861 gcgcttgcgg aaccagctca ggtcttcgcc gaccgccagc gtcgcgct
//
LOCUS       DAOPZO010000209         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_90698_length_1907_cov_12.767279, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000209 DAOPZO010000000
VERSION     DAOPZO010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90698_length_1907_cov_12.767279"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /gene="corA"
                     /locus_tag="PKI68_09250"
     CDS             <1..540
                     /gene="corA"
                     /locus_tag="PKI68_09250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNX53916.1"
                     /translation="FPLLESYDSRLDDMEEVILKHPDSSSVALLFAVKRDLMTLRRTL
                     WPVREMASALVNSASTLITPPTRIYLRDCQDHAVRLLDLAETCRELESSLMDFYLSTI
                     SNRMNEIMKVLTIISTIFIPLTFIAGVYGMNFKHMPELETHNGYFIVLGLMAILGLGM
                     FGVFVHRGWLVPGRRRTEK"
     gene            complement(587..1831)
                     /gene="fabF"
                     /locus_tag="PKI68_09255"
     CDS             complement(587..1831)
                     /gene="fabF"
                     /locus_tag="PKI68_09255"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNX53917.1"
                     /translation="MSRRKVVITGMGAVTPIGNTLAEFWEGCKTGRSGGARITQIENL
                     EGFTTTIAAEVKNFNPELYISKKEVRKMDLFTQYGLAASIMAVQDSGLDFTKEDLERI
                     GVIVSTGIGGLQIMEASQDIYRERGPGRLSPFVIPVMITNIVAGHVAIQYGLKGPNFC
                     VTSACASGTHSIGEALRIVQHGEADVMITGGTEGAITPLGVGGFCALRALTTRNDDPA
                     RASRPFDLNRDGFLIGEGAGVIVIESEEHAKARGAKIYCELAGYGRTCDGHHITAPDP
                     DATQAARGMKLAFEDAGLKAEDIDYINAHGTSTALNDKGETLAIKKALGEAQARKVAV
                     SSTKGMTGHMLGAAGGVEAIVCAMAISEGIIPPTINYETPDPDCDLDYVPNVARKTEV
                     RAALSNSLGFGGHNATLCFKKA"
BASE COUNT          362 a          535 c          577 g          433 t
ORIGIN      
        1 ttcccgctgc tggaatccta cgactcgcgg ctcgacgaca tggaagaagt cattctgaag
       61 catcccgact catcgtctgt cgcgctgctg tttgcggtga agcgcgacct gatgacgctg
      121 cgccgcacgc tctggccggt gcgtgaaatg gccagcgctc tggtgaactc cgcctcgacg
      181 ctgatcacgc cgcccacgcg gatttatctg cgcgactgcc aggaccatgc ggtacgcctg
      241 ctcgatctgg cggaaacctg ccgtgaactg gagtccagcc tgatggactt ctatctatcc
      301 accatcagca accggatgaa tgaaatcatg aaggtgctga cgatcatttc gacgatcttc
      361 attccgctca cttttatcgc cggtgtgtac ggcatgaatt ttaaacacat gccggaactg
      421 gaaactcaca acggttactt tatcgttctg gggctcatgg caattctcgg cctcggaatg
      481 ttcggcgtct ttgttcaccg gggctggctg gtgcccggcc gccgccggac ggaaaaataa
      541 aaggccgctc ctgagagcgg ccttcgtttc cgaaaacaca aaacccttac gcttttttga
      601 agcagagggt ggcgttgtgt ccgccgaaac cgagcgagtt gctgagcgcg gcgcgcactt
      661 ccgttttgcg ggcgacgttc ggaacgtagt ccagatcgca atccgggtcg ggcgtttcgt
      721 agttgatggt cggaggaata attccttcgg agatggccat tgcacagacg atcgcttcaa
      781 cgccgccggc ggcgccgagc atgtgtccgg tcatcccttt ggtggagctg acggccactt
      841 tgcgggcctg cgcttcgccc agcgcttttt tgatggccag cgtttcgccc ttgtcgttca
      901 gggcggtgct ggtgccgtgg gcgttgatgt agtcaatgtc ttcggctttc aggccggcat
      961 cttcgaaggc aagcttcata ccgcgggcgg cctgagtggc atccggatcc ggagcggtga
     1021 tgtgatgacc gtcacacgtg cggccgtagc cggcgagttc gcagtagatt ttcgcgccgc
     1081 gcgctttggc gtgttcttcg ctttcgatca cgatgacgcc ggcaccttcg ccgatgagga
     1141 acccgtcgcg gttgagatcg aacggacgcg acgcgcgggc cggatcgtca ttgcgggtgg
     1201 tgagcgcgcg caaggcgcag aagccgccga cgccgagcgg cgtgatcgca ccttcggttc
     1261 cgccggtgat catgacatcg gcttcgccgt gctggacaat gcgcaaggct tcgccgatgg
     1321 agtgcgtgcc ggaagcgcag gcgctggtga cacagaaatt aggacctttg aggccgtatt
     1381 gaatcgcaac atgaccggcg acgatattcg tgatcatgac cgggatgacg aacggagaaa
     1441 ggcgtcccgg gccgcgttcg cggtagatgt cctgcgaagc ttccatgatc tgcaggccgc
     1501 cgatgccggt gctgacaatc acgccgatgc gttcaagatc ttctttggtg aagtccaggc
     1561 cggaatcctg aaccgccatt atagaggccg ccagtccgta ctgggtgaag aggtccattt
     1621 ttcggacttc tttcttcgaa atgtacaact cgggattgaa gtttttgact tcggcggcaa
     1681 tcgtcgtggt aaagccttcg agattttcaa tctgggtgat gcgcgcgccg ccggagcggc
     1741 cggttttaca gccttcccag aactcggcga gggtgttgcc gatcggggta actgcgccca
     1801 tccctgtgat aactactttg cgacgactca tgattctcct ccggaaattg aacaatacaa
     1861 aaaaaagccg ggaactcggt taaaagttcc cggtttttct atcacaa
//
LOCUS       DAOPZO010000210         1863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_94081_length_1863_cov_6.279314, whole genome shotgun sequence.
ACCESSION   DAOPZO010000210 DAOPZO010000000
VERSION     DAOPZO010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94081_length_1863_cov_6.279314"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PKI68_09260"
     CDS             <1..659
                     /locus_tag="PKI68_09260"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HNX53918.1"
                     /translation="KFIAESLAVYEAHSNQPQTDDVSMLSPYLHFGQISPLYIALEIQ
                     KADPKSRFLEQLIVRRELAMNFVWFTPDYDKFSCLPDWAKLTLKNHAKDKREYLYTRE
                     ELEQSQTHDPYWNAAMTEMRVTGFMHNYMRMYWGKKVLEWSRSPEEAFETLLAINNKY
                     FLDGRDPNSCAGVAWVFGKHDTAWKERPIFGKTRYMNAAGLKRKCDIEGYVEKVKAIG
                     "
     gene            complement(701..961)
                     /locus_tag="PKI68_09265"
     CDS             complement(701..961)
                     /locus_tag="PKI68_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53919.1"
                     /translation="MTYKLVEDERVNITFHYKDGTSVHYQKIYFFNMPYFPLLIFNGL
                     NSAIVIDKERIESVSVKDKEYSIDRLDIEKGTENQLLHLKEH"
     gene            complement(1329..>1863)
                     /locus_tag="PKI68_09270"
     CDS             complement(1329..>1863)
                     /locus_tag="PKI68_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53920.1"
                     /translation="YEGTFEIAQDEGRNVMKQMVPKPGCLWHKGTHGACIALYGGCDA
                     VSQFAIQAETKIADGYVMLGGDILGGRTVEKAVVALKLSKTGEWGFIPGKLSGKIQNF
                     DPAAWHSMEVRLFNGVATGRIDGQQLFETAVTNKTPMTIAITSSYHPNLFRNIEVQPG
                     TPGSQGKSAAEPVQEEP"
BASE COUNT          401 a          473 c          530 g          459 t
ORIGIN      
        1 aaaaattcat cgctgaaagc ctggcggttt acgaagcgca cagcaatcag ccgcaaaccg
       61 acgatgtttc catgctcagt ccgtatctgc actttgggca gatttcgccg ctctatatcg
      121 cgctcgaaat tcagaaggcc gatccgaaga gccgctttct cgaacagctg atcgtccgcc
      181 gcgagctggc gatgaacttt gtctggttca cgccggacta cgataaattt tcctgcctgc
      241 ccgactgggc gaagctgacg ctgaaaaacc acgccaagga taagcgggaa tatctctaca
      301 cccgcgaaga gctggaacag tcgcagacgc acgatccgta ctggaacgcc gcgatgaccg
      361 aaatgcgcgt caccggcttt atgcacaact acatgcggat gtactgggga aaaaaggtat
      421 tggagtggag ccgctcaccc gaagaggcat ttgaaacact gctcgccatc aacaacaaat
      481 attttctcga tggccgcgac ccgaactcct gcgccggagt cgcctgggtc ttcggcaaac
      541 acgacaccgc gtggaaagag cgtccaatct ttggcaaaac ccgctatatg aacgccgccg
      601 gtctcaagcg gaagtgtgat attgaggggt atgtcgaaaa ggttaaagcc atcggctaaa
      661 tgctggatac gggtgatgaa cggaaccctt tttgtgggta ttagtgttct ttgagatgaa
      721 gaagctggtt ttcggttcct ttttcaatat caagtcggtc gattgaatat tccttatctt
      781 ttactgagac gctctcgatt ctttctttat ctatgacgat ggcggaatta agcccgttaa
      841 aaatcagtag aggaaagtat ggcatgttga aaaagtagat tttttgatag tggactgatg
      901 tcccgtcttt atagtgaaag gtaatattta cacgttcatc ttcaactagt ttgtaagtca
      961 tagactctcc ttggtgaatt ttaatgacac ggtgttgttg aacgggtggc agtgtagcag
     1021 gtttccggac cttggaaagt ttgcggcgga tttttaacaa tgttgtagcc gaggtccatg
     1081 acctcggtta attccgcgat catggatcgc ggctacaatg gtggagcggg cactccgtgc
     1141 acgcttgaac gcgcccgacc tttcgatgaa ctcagggcag gcgggcgcgt tccaccttga
     1201 tcagctggtt gcgttgaaac cttcatggaa ggtgacgggg cagcccacag gtgctgcgga
     1261 tcggctgaac ttcagcgttc acgctgtgga acggcgaacc gtccagagcg tgagcgcttg
     1321 gttctctctt acggttcttc ctggacgggc tctgcggcgg acttgccctg gcttccgggg
     1381 gtgccgggct gcacttcgat gttgcggaac aggttgggat ggtagctcga tgtgatggcg
     1441 atggtcatcg gagtcttgtt ggtgaccgcc gtttcgaaga gctgctgtcc gtcaatccgg
     1501 ccggtcgcaa cgccgttaaa caggcggact tccatcgagt gccaggccgc cgggtcgaag
     1561 ttctggattt ttccggagag ctttccgggg atgaagcccc attcgccggt cttgctcagc
     1621 ttcaaggcga cgacggcctt ttcaactgtt cttccgccca gaatatcgcc gcccagcatg
     1681 acgtatccat ccgcgatttt cgtctcggcc tggatggcga actgggagac ggcgtcgcat
     1741 ccgccgtaga gggcgatgca tgcgccgtga gtgcctttgt gccagaggca gccgggcttg
     1801 ggcaccattt gcttcatgac gttccggccc tcgtcctgtg cgatttcgaa cgtgccttca
     1861 tag
//
LOCUS       DAOPZO010000211         1803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_99108_length_1803_cov_10.891876, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000211 DAOPZO010000000
VERSION     DAOPZO010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99108_length_1803_cov_10.891876"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(556..1798)
                     /locus_tag="PKI68_09275"
                     /pseudo
     CDS             complement(556..1798)
                     /locus_tag="PKI68_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610453.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          347 a          519 c          461 g          476 t
ORIGIN      
        1 ccgcgctcta catgaaaaac catcccggcg tttccgccgc cgacaccgtt tccgcgctca
       61 aaacagccgc cggaccgacc ggcttcctca ccgaatccgc cgtcaaacag tttctcgtta
      121 aatagctttc cttgcgcgga ggcgtataag tcgggccgta tttatagagc attcgtccca
      181 gaatttcttc tatagtcgtc ggatttttgt actttgacat ggcgttgcct cctttcgcga
      241 tatacccatt acaaggtgat tttctctagg aagccgttgg cttcattgga ataaacgcgg
      301 cgtagtttgc gccggattcg tggatgtttt tcggatcggt ctttgttttt ttgattcggt
      361 ttcttcgacg tcatcgcttc atatctcctc tacaatagtc gctcctgaaa aaccccggtt
      421 tcacaaaacc acaaaagccg gattcagttg attcaaactg actatttctg acggcagtgt
      481 ccgcaagacc tgcttgagaa cctcaaacag gtgtgcaaag accaagcgca agaacgcagg
      541 aagcagggag gctgccttca gtagcttcct gaagttcatt cccgccgcac tcaggatcgc
      601 attgatttga ccgccttcga ttcctttgag ctggcatcga tccatccggt gttcccgttt
      661 taagtggccg atccccggct cgatcgcggc ccggcgtttc atccatttcc aaagactgcg
      721 cgccgtgccg ccccgacggc gtttgtccac atgcacttca cagggtccct cgtagttgtg
      781 gccgcgatag cccatatcca caaaaccatg tccgggcatt gcgcgatgcg ctcaacctgt
      841 ccaagggcat cgcagagcgt gtgtccgtcg tacggattgc cgtgcaccgc cttggctccg
      901 acgaaccacc cgcccttgct ggtcgccgcc acactgacct tgcatccgaa ctcatagcgt
      961 ttatgggctt tgcctttgct gatgcattcc acctgcggct catgaacact gtaaatcttt
     1021 cccttgtctg tccgttgttg ttcctgaatg tgccgggcga cagccagcag attgcgcagc
     1081 tcatcgtcgc tcacgggagc tttgcgttca atatcccgaa tgacccgtcc aaggatggtg
     1141 cgcagtttcc gggtagagtt gcgcgctctt ttcatctggc gggcgtgagc ataacgatga
     1201 ctcatcagca gatgatgttt cgctgtgcgg ttgtagttct gccgcaaatc aattccgcgt
     1261 tcggcggccg cttttaccag ccgttcccgt gaacgctcgt aaagccggga atcggccgga
     1321 tagcgaacct ctttttcctg aacggtggtg tccatattga cccgcttgag ttgattcggt
     1381 gtgatgacct tgagcttgag cccggccgca atcgtttcct tgagcagttc ttccgcaccg
     1441 gcctcgccga tccggttgcg gaagcgcgtc atgctggtcg gatcaatcgg cttgctgtgt
     1501 tcaaagtact ggtttccgct gaagtgctgc cagtacgggt tttcgaccca ggcttccacc
     1561 acgtcctcgt cactgaggtt gaaggtatat ttgaggtaat gcaaagcaac catcagccgg
     1621 gtgctggccg ccgggcgccc gttgtcttcg caataggttt tcccgaacag ttcgtcgagc
     1681 cgctcccaat cgacttcttg gccagacgga ccagcggatg attcaaatca atgatgcgga
     1741 gaagttcagt tttgaacaga tcgccctgcg gattatgtgg agttttaacc ggtttcattg
     1801 ata
//
LOCUS       DAOPZO010000212         1712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_107525_length_1712_cov_9.893180, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000212 DAOPZO010000000
VERSION     DAOPZO010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107525_length_1712_cov_9.893180"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1566)
                     /gene="thiC"
                     /locus_tag="PKI68_09280"
     CDS             complement(40..1566)
                     /gene="thiC"
                     /locus_tag="PKI68_09280"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF006763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNX53921.1"
                     /translation="MKQYSDYGPFFPHSRKVYVNGTQPGVRVPFREIELAHTINEDGS
                     ENRNAPVRVYDTAGPNTEGPLPALRAPWIAGRPNATQLELARAGTISPEMEFAAIRES
                     VAPEFVRDEIAAGRAIIPVNRKHPECEPMIIGRKFRTKINANIGNSALGSSIPEELEK
                     MRWSILWGADTVMDLSTGKEIHATREAILRHCPTPIGTVPLYQALQKVGGDVNALTWE
                     IYRDTLIEQAEQGVDYFTIHAGVLRKHIPLAARRLIGIVSRGGSIMAQWMQNKNEESF
                     LYTHFEEICQILQKYDIAFSLGDGLRPGCLADANDDAQFGELTTLGELTQIAWRHGCQ
                     VMIEGPGHVPMHKIKENMDRQLADCFEAPFYTLGPLVTDLAAGYDHLNSAIGGAMIGW
                     YGCAMLCYVTPKEHLGLPNKEDVREGVVAHKIAAHAADLAKGIPNAQERDNAISKARF
                     EFRWEDQFNLCFDPERARRFREQSMPPGEKFDVKQRYCSMCGEKFCSMSINHKLRKET
                     "
     regulatory      complement(1601..1690)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT          269 a          460 c          595 g          388 t
ORIGIN      
        1 gaaacctgaa aggggaattc aaatttcaag tttcagattt caagtttcct tccggagttt
       61 gtgattgatg ctcatggagc agaacttttc gccgcacatg gagcagtagc gctgtttgac
      121 gtcgaatttt tcgcccggcg gcatggactg ttcgcggaag cggcgcgccc gttccgggtc
      181 gaagcagagg ttgaactggt cttcccagcg gaattcaaag cgggctttgc tgatggcgtt
      241 gtcgcgttcc tgcgcgttgg gtatgccttt ggcgaggtcg gcggcgtgcg cggcgatttt
      301 atgggcgacg acgccttcgc ggacgtcctc tttgttcggc aggccgaggt gctctttcgg
      361 tgtgacgtag cagagcatgg cgcagccgta ccagccgatc atggcgccgc cgatggcgct
      421 gttcaggtgg tcgtagccgg cggcgaggtc ggtgacgagc gggccgaggg tgtagaacgg
      481 cgcttcgaag cagtcggcca gctggcggtc catgttttct ttgatcttat gcatcggaac
      541 gtggccgggg ccttcgatca tcacctggca gccgtgcctc cacgcaattt gcgtcagctc
      601 gccgagcgtc gtgagttcgc cgaactgggc gtcgtcgttg gcgtcggcga ggcagccggg
      661 gcgcaggccg tcgccgagcg agaaggcgat gtcgtatttt tgaaggattt gacagatctc
      721 ttcgaagtgc gtgtagagga agctctcttc gtttttgttc tgcatccatt gcgccatgat
      781 ggagccgccg cggctgacga tgccgatgag ccggcgggcg gcgagcggaa tgtgtttacg
      841 gagcacaccg gcgtggatgg tgaagtagtc gacgccctgt tcggcctgct cgatgagtgt
      901 gtcgcggtag atttcccagg tgagggcgtt gacgtcgccg ccgactttct ggagcgcctg
      961 atagagcgga accgtgccga tcggcgtcgg gcagtggcgc aggatcgctt cgcgcgtcgc
     1021 gtgaatctct ttgccggtgg agaggtccat gacggtgtcc gcgccccaga gaatcgacca
     1081 gcgcattttt tcgagttctt ccgggatgga cgagccgagg gccgagttgc cgatgttggc
     1141 gttgatcttg gtgcggaact tccggccgat aatcatcggt tcgcattccg gatgcttgcg
     1201 gttgacggga atgatggcgc ggccggcggc gatttcgtcg cggacaaatt ccggcgcgac
     1261 cgattcgcgg atggcggcga attccatttc gggcgagatg gttccggcgc gggccagttc
     1321 cagctgggtg gcgttcgggc gtccggcaat ccagggtgcg cgcagggcgg gcagcgggcc
     1381 ttccgtattc ggcccggcgg tgtcgtaaac gcggaccggc gcgttgcggt tttcgctgcc
     1441 gtcttcattg atcgtatgcg ccagttcgat ttcgcggaac gggacgcgta cgccgggttg
     1501 cgtgccgttg acatacacct tccgcgagtg cgggaaaaac ggtccgtagt ctgaatactg
     1561 tttcatcgct cgtttccttc ctatagcggg aagggagcgg caaaggaacc gttcgcttcc
     1621 ctccgcggca tgacccgcac aggttcaaag ggttgtttct cagcccgttc gggcaccccc
     1681 agcaaaggcg gcactatact gcttccggcg gg
//
LOCUS       DAOPZO010000213         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_113841_length_1651_cov_6.777569, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000213 DAOPZO010000000
VERSION     DAOPZO010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113841_length_1651_cov_6.777569"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PKI68_09285"
     CDS             complement(<1..556)
                     /locus_tag="PKI68_09285"
                     /inference="COORDINATES: protein motif:HMM:NF036808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53922.1"
                     /translation="MNSVILKKGREKNLLQRHPWIFSGAIERVEGAPGVGETVTVCSF
                     DRKPLAQAAWSPESQIAARVWSFDPETVIDAAFFRAKLQNAISARCHLTGRACSPSAP
                     LVDERTARRSVPTTNAVRLVAAESDGLPGLIIDRYADWIVCQFLSAGSERWRETIVQI
                     LQELFPGQSIYERSDVSVREKEGLP"
     gene            complement(600..1436)
                     /locus_tag="PKI68_09290"
     CDS             complement(600..1436)
                     /locus_tag="PKI68_09290"
                     /inference="COORDINATES: protein motif:HMM:NF005395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX53923.1"
                     /translation="MTNVIVVIGAGSIGQAIARRVSAGKQVLLADLREENADAAAKVL
                     HDAGFEVSTAIVDVSSRKSVRALVETAASMGSITGVIHAAGVSPSQASPATILKVDLY
                     GTALVLDEFGNVIARGGAGLVIASQSGHRLPPLTPDQNKALATTPADDLLFLPMLQPE
                     QVKDSLHAYQISKRGNSLRVMAEAVRWGKRGARVNTISPGIIFTPLARDELNGPRGAG
                     YRRMIEASAVGRGGTPDEVGAVGALLLGPDGAFITGSDFLMDGGVTAAYWYGELAPEF
                     GK"
BASE COUNT          380 a          505 c          443 g          323 t
ORIGIN      
        1 acggcagacc ttccttttcg cgtaccgaaa catcggagcg ctcataaatg gactgaccgg
       61 ggaaaagttc ctgcaggatt tgcacaatcg tctctctcca gcgttcggaa ccggcggaaa
      121 gaaactgaca gacaatccag tcggcgtagc gatcaataat caggccgggc agcccgtcgg
      181 attccgccgc caccagccga accgcattgg tggtagggac ggatcgccga gccgtccgtt
      241 cgtcgacaag cggcgcgctc ggcgagcacg ccctacctgt tagatggcaa cgggcactga
      301 tcgcgttttg cagtttcgcg cggaaaaacg cggcgtcgat cactgtttcc ggatcaaaac
      361 tccagacacg cgccgcgatc tgcgactccg gcgaccaggc ggcttgcgcc agcggcttgc
      421 ggtcgaacga acaaaccgtc accgtttcgc cgacacccgg cgcgccttca acgcgttcaa
      481 tcgcgccgga aaaaatccac ggatgccgct gaagcagatt tttttcgcgg ccttttttta
      541 gaatgacaga attcatgcca gagttcttaa ctgtagagtg gaatcaagtc gatctgtttt
      601 tattttccga actccggagc gagttcaccg taccagtagg cggcagtaac tccgccatcc
      661 atgagaaagt cgctaccggt gatgaacgcg ccatccggcc ccagcaagag cgccccaaca
      721 gccccaacct catccggggt accaccacgc ccgaccgcag atgcttcgat cattcgccgg
      781 tatcccgcac cgcgaggacc attcagctca tctctggcta gcggcgtaaa gatgatgccg
      841 gggctgatcg tattgaccct cgccccccgt ttaccccatc gcaccgcctc ggccatcaca
      901 cgcaacgaat tgccgcgctt ggaaatctgg taagcgtgga gtgaatcttt cacctgttcg
      961 ggctgaagca tcggaagaaa gagtaaatca tcggctggcg tagtcgctaa cgccttgttt
     1021 tgatcgggag tcaacggcgg caggcgatgc cccgactgcg aagctataac aaggccagcg
     1081 ccaccccgcg cgatgacatt gccgaattca tcaagcacaa gtgcagtacc atacaaatca
     1141 accttcagga tcgtggctgg cgacgcctgt gatggagaaa caccggcggc atgaatgact
     1201 ccggtaatgg accccatcga tgcggctgtc tcgacaagag cacgaaccga cttgcgcgag
     1261 gagacatcga cgattgcggt gcttacctca aatccggcat catggagaac tttagcagcc
     1321 gcatcggcat tttcctcacg caagtcagca agcagaactt gcttacctgc gctgacccgc
     1381 ctcgcgatcg cctgcccgat cgatccagca ccaatgacga cgataacgtt agtcattgca
     1441 acttcctttc ttaaaccagc caaaacagat gttcagcaga cgatgcgggc gaacttgcgc
     1501 ttgccgactt tgaggatcat gccgtcgaca ggagtaatga cggctttgac gtcagaaacc
     1561 tgttcgtcgt taatgcgcac cccaccctgc tgaatgaggc ggcgggcttc gctgctcgac
     1621 ggcgcgaagc cggctttcat gacgaggtcg a
//
LOCUS       DAOPZO010000214         1647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_114281_length_1647_cov_8.762563, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000214 DAOPZO010000000
VERSION     DAOPZO010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114281_length_1647_cov_8.762563"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..629)
                     /gene="msrB"
                     /locus_tag="PKI68_09295"
     CDS             complement(228..629)
                     /gene="msrB"
                     /locus_tag="PKI68_09295"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNX53924.1"
                     /translation="MKQSFPVQKTDAEWKKELTSEQYRVLRQAGTERPFTGIYTDNDK
                     PGVYRCAACGAELFASGSKFHSGCGWPSFDAAAKAGTVVLRPDNSLGMTRTEVLCANC
                     GSHLGHLFDDGPTATGLRYCINSEALKFEEK"
     gene            complement(747..1514)
                     /locus_tag="PKI68_09300"
     CDS             complement(747..1514)
                     /locus_tag="PKI68_09300"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNX53925.1"
                     /translation="MGKGLILFDIDGTLLHAPGGRAAFAQAFKEAFGWEQGVEHINFY
                     GATDLNVFRGICVERGVESTPDMERAFFARLAPALETRLAVNPPTLFPNVENILKRLT
                     GIPYNPPRPSLCSGHPSRGGEFLKSPPLEGCPQGGVGCNWKLGLVTGNIEATAWAKLR
                     YAGIDRYFSFGGFGCTYADRAEIARQALRASGVERPEKVFLIGDTPSDVNAAKVNGFT
                     AIAVATGGFDVPALEKAGADFVFEDLTDTERVLSVFE"
BASE COUNT          381 a          450 c          454 g          362 t
ORIGIN      
        1 atgccgctcc agatgatgag cccgcagggc ccgctgacaa tcatcgtcaa agaaatcaag
       61 gaagatgcgg ttacggtcga tgcgaaccat ccgatggcag gcaaagacct gacgttcgaa
      121 ctggagctgg tcgaaatcgt ctaggagatt ttaaccacta atcttcacta attctcacta
      181 atcaggattt ccaattgatt agtgccgatt agtggaaatt agtggattca tttttcctca
      241 aacttcagcg cctcggagtt gatgcaatag cgcaggccgg tggcggtcgg gccgtcgtcg
      301 aagagatgtc cgagatggct tccgcagttc gcacaaagca cttcggtgcg ggtcattccg
      361 aggctgttat cgggccgaag aacaacggtt cccgctttgg ctgctgcgtc aaaactcggc
      421 cagccgcatc cggaatggaa tttggagccg gacgcaaaca gctccgcgcc gcaggcggcg
      481 cagcggtaaa cgccgggctt gtcgttgtcg gtatagattc cggtgaacgg ccgctcggtt
      541 ccggcctgac gtaaaacgcg gtactgttcg gacgtcagct ctttcttcca ttcggcgtca
      601 gtcttctgaa cagggaaact ttgcttcata ggtgcctcct tctgttttgt gcaaccaggg
      661 aagaatgtga gagcaagtgc gtaaatgagt atgtgtgtaa taaaattcca atttgcctgt
      721 ttcatattcg tcacccccat actcacttac tcaaacacag acaacactct ctctgtatcc
      781 gttagatctt caaagacaaa atcggcgccg gctttttcca gcgccggaac atcgaacccg
      841 ccggtggcga ccgcaatggc ggtaaaccca ttcactttgg cggcgttgac atcgctcggt
      901 gtgtcgccga tcagaaaaac tttttccgga cgctctacac cggaagctcg tagcgcctgc
      961 cgggcgattt cggcgcggtc ggcgtacgta cagccgaagc cgccgaagct gaagtaacga
     1021 tcaattcctg cataacggag cttagcccac gccgttgctt cgatgttgcc ggtaacaaga
     1081 ccgagcttcc aattacaacc caccccgccc tgcgggcacc cctccaaagg aggggatttt
     1141 aaaaattccc ctccgcggga ggggtggccg gagcaaagcg atggccgggg tgggttatat
     1201 ggtattccgg tcaagcgctt cagaatgttt tcaacgttcg gaaaaagagt cggcggattt
     1261 acggccagcc gggtttcgag cgccggcgca agccgcgcga aaaaggcgcg ttccatgtcc
     1321 ggcgtcgatt ccacgccgcg ctcgacacag ataccgcgga aaacattgag gtcggtcgcg
     1381 ccgtaaaagt tgatatgttc gacgccctgc tcccagccga acgcctcttt gaaggcctgc
     1441 gcgaaagcgg cgcggccgcc cggcgcgtga agcagggttc cgtcgatatc aaagaggatc
     1501 agtccttttc ccataaattc tgtttccaag gcggtcgcga gctgttaaaa acgccgtctt
     1561 aaatttcccg gattctaaag gaagaaagca ggttggcatg tctaaaacgt acaaaattac
     1621 tgtgatgccc ggcgacggaa tcggcac
//
LOCUS       DAOPZO010000215         1622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_117150_length_1622_cov_4.418634, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000215 DAOPZO010000000
VERSION     DAOPZO010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117150_length_1622_cov_4.418634"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PKI68_09305"
     CDS             complement(<1..622)
                     /locus_tag="PKI68_09305"
                     /inference="COORDINATES: protein motif:HMM:NF036808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53926.1"
                     /translation="MNSVILKKGREKNLLQRHPWIFSGAIERVEGAPGVGETVTVCSF
                     DRKPLAQAAWSPESQIAARVWSFDPETVIDAAFFRAKLQNAISARSQIGGLVVQPTPA
                     FGHPSDGGDSVSSTGNPLLGRGAGTAGRVSFEKNSSNAVRLVAAESDGLPGLIIDRYA
                     DWIVCQFLSAGSERWKDTIVQILQELFPGQSIYERSDVSVREKEGLP"
     gene            complement(736..1041)
                     /locus_tag="PKI68_09310"
     CDS             complement(736..1041)
                     /locus_tag="PKI68_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53927.1"
                     /translation="MITTIITSIITAAITGLITFALEERKLKTELRTEFMAEQVAKKL
                     LENKKWTKRSFSEIKKRLGGFDDDELRKILVRAGAIRFESEYLDEVWGLISRNEDSI"
BASE COUNT          366 a          450 c          395 g          411 t
ORIGIN      
        1 acggcagacc ttccttttcg cgtaccgaaa catcggagcg ctcataaatg gactgcccgg
       61 ggaaaagttc ctgcaggatt tgcacaatcg tgtccttcca gcgctccgaa ccggcggaca
      121 gaaactgaca gacaatccag tcggcatagc gatcaataat caggccgggt agcccgtcgg
      181 attccgccgc aaccagccga accgcgttgg aagaattctt ctcgaaagaa accctccccg
      241 ccgtcccggc acccctccca aggaggggat tacccgtact ggataccgag tcccctccat
      301 cggaggggtg gccaaaggcc ggggtgggtt gaacaactaa gcccccgatt tgcgaacggg
      361 cactgatcgc gttttgcagt ttcgcgcgga aaaacgcggc gtcgatcact gtttccggat
      421 caaaactcca gacacgcgcc gcgatctgcg actccggcga ccaggcggct tgcgccagcg
      481 gcttgcggtc gaacgaacaa accgtcaccg tttcgccgac acccggcgca ccttcgacgc
      541 gctcaatggc cccggaaaaa atccatggat gccgctgaag cagatttttc tcacggccct
      601 tttttagaat gacagaattc atgcccgcgt tcttaaacat tgcgttagat caagtcgact
      661 ttaaaccgct ggcgaacgtc ttacgtcact ctgagcgggc ggagtccgcg atagagtgaa
      721 tgcggtggtt ggcaattata tactatcttc atttcttgag attaatcccc acacttcatc
      781 tagatattca gactcaaacc gaatagctcc agctcggact aaaatttttc ttaattcatc
      841 gtcatcgaat ccaccaagtc gtttcttgat ctcactaaaa cttcgcttcg tccatttctt
      901 attttctagt agtttttttg ctacctgttc agccataaat tcggtgcgca gttctgtttt
      961 cagttttcgc tcttcgaggg caaatgttat cagacccgtg atggcagctg taatgattga
     1021 agtaataatg gttgttatca tatcgcttcc ttatgttctg cgttagtggt agttccctga
     1081 ttttttgtgt tttcttgcca acatttaatg aatgaccggt cactttttcg agccgggagc
     1141 ctattccaag tccggctgga ggtcaataaa cggccttatt accgcgcctg ccgttcgctc
     1201 tgcgcttcca tatattggaa accgaaaggt ggagcgaaca cttccgcagt cgccacaagg
     1261 ctatggcggg acacagccgt gtacgctttt tttattaaac gcgctcggag atcgcgttcc
     1321 acctcattcg aggcgttttc aacagaagga aacgaaggga accgaggaga gatatccgca
     1381 atcaccggct ggtgagccaa ctttgttatc cttcgttctc ttctgtaaaa aatccctctg
     1441 cacttaaact ttaatgcggg cgaacttgcg tttgccgact ttcagaatca tgccgtcaac
     1501 cggcgtgaca accgctttga cgtcggagat ctgttcgtcg ttgatgcgca ccccgccctg
     1561 ctgaatcagg cggcgggctt cgctgctcga cggcgcgaag ccggctttca tgacgaggtc
     1621 ga
//
LOCUS       DAOPZO010000216         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_126189_length_1547_cov_3.865952, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000216 DAOPZO010000000
VERSION     DAOPZO010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126189_length_1547_cov_3.865952"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PKI68_09315"
     CDS             <1..401
                     /locus_tag="PKI68_09315"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX53928.1"
                     /translation="VYSGAKMKTADVIRWLEERRALNADYFERIFDRMDASTGEVVDD
                     FSQLGRMLILNQKLMEEMGLCNAALAEIIAGFREQGVPAKISGSGLGDCAVGLGRINF
                     PIKKYPSYLLDVSAEGFFFEEAAVDSVISL"
     gene            complement(329..>1547)
                     /locus_tag="PKI68_09320"
     CDS             complement(329..>1547)
                     /locus_tag="PKI68_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNX53929.1"
                     /translation="SFTTPPKSNPNPTDTPKASDWQQADAYIAPDFYAFFPNDPEGGK
                     ALDSLWRAADRDSRSDKEILETVRNGLRCTKEHKTLILGWLGNRYILGKSPQNPDAIE
                     IMYHASDFSGENADPQGIRHYAVYFGLSVVQPKTPAILRTLADLCMRVDDPNDLDRVA
                     WGTSDQKQELLNYLEPYLQSKDTAVHNKAIVLEQIFTGKLKAFDWARQRAEERASAKY
                     AGRLEDIRTILHTGSSQQRKDTLNLILRERIFLIMDESFVGSFAACATDSDATVRRTA
                     TSLIGEQWVWKGPVQSSEAIELLLRLSKDADRQVRYNAVYYGLSTVRQKNEPVVRRLL
                     EMAFDDRERNLYGRIAWGLRGSRTTTARLLDEYIAGNNPHFAQCAREIYKDMTESTAA
                     SSKKKPSADTSSK"
BASE COUNT          331 a          378 c          431 g          407 t
ORIGIN      
        1 cggtttattc cggcgcgaag atgaagacgg cggatgtgat ccgctggctc gaagaacgcc
       61 gcgccctgaa cgccgattat ttcgagcgca tcttcgaccg catggacgcc agcaccggcg
      121 aggtggtgga tgatttttcg cagctgggcc ggatgctgat tctgaaccag aagctgatgg
      181 aagaaatggg gctctgcaat gccgcgctgg cggaaatcat cgcagggttc cgggagcagg
      241 gcgtgcccgc taaaatttcc ggctccggcc tcggcgactg cgccgtcggg cttgggcgta
      301 ttaattttcc gatcaagaag tatccgagct acttacttga tgtgtccgcc gaaggcttct
      361 tttttgaaga ggcggcagtg gactctgtca tatctttgta gatttcgcgg gcgcactgtg
      421 cgaaatgcgg attgttcccg gcgatatatt catccaaaag gcgggcggtt gtagtccggc
      481 ttccgcgcag tccccaggca attcttccgt aaagattgcg ctctctgtca tcaaaggcca
      541 tttcaagcaa ccggcgtaca accggttcat ttttttgccg cacggtggat aagccgtaat
      601 acactgcgtt ataacgaacc tgcctgtcag cgtcttttga gaggcgaagt aaaagctcga
      661 tggcttcgga agattgtaca ggacctttcc acacccactg ttcaccaatc agagaagttg
      721 ctgtcctgcg taccgtcgcg tcggaatcgg ttgcacatgc cgcgaaggaa ccgacaaagg
      781 actcgtccat aatgagaaag attctctcac gcaggatcag gtttaaagtg tcttttcgtt
      841 gctggctgct tcctgtgtgc aaaatggttc ttatgtcctc cagtcgtcct gcatattttg
      901 cagacgcccg ttcttcagcg cgctgtctgg cccagtcgaa ggctttcagt tttcctgtga
      961 agatctgctc aagaacaatg gccttattat gaacggctgt gtctttggat tggagatagg
     1021 gttccaaata attaagcagt tcctgtttct ggtctgaggt gccccaggcc acccgatcaa
     1081 gatcgttggg gtcgtcaacc ctcatgcaaa gatcagccag tgtgcgaagg atagctggcg
     1141 ttttcggttg tacgacagac agaccgaaat aaaccgcgta gtgacggatg ccttgagggt
     1201 ctgcattttc accgctgaag tctgacgcat gatacatgat ttcaatagca tccggattct
     1261 gaggcgattt tcccaagatg tagcggttgc cgagccatcc aaggattaag gtcttatgtt
     1321 ctttcgtgca gcgcaggccg ttacgcactg tttcaaggat ttctttgtcc gacctggagt
     1381 ctctatcggc tgcgcgccag agggaatcta atgcctttcc gccctccggg tcattcggaa
     1441 agaatgcata aaaatcgggt gcgatataag catctgcttg ttgccaatcg gaggcttttg
     1501 gtgtgtcagt tgggtttgga ttgcttttcg gtggagtagt aaaggat
//
LOCUS       DAOPZO010000217         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_127166_length_1539_cov_10.082884, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000217 DAOPZO010000000
VERSION     DAOPZO010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127166_length_1539_cov_10.082884"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..>1539
                     /locus_tag="PKI68_09325"
     CDS             272..>1539
                     /locus_tag="PKI68_09325"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNX53930.1"
                     /translation="MKVVKFGGTSVANAEQISKIVKIVTADADRRIVVVSAPGKRNKA
                     DTKVTDLLIALANTVIAGKDFEAGLMAVVNRYAEIQRDLKLSETVVKTIEADLRGRIA
                     RRGKNDAQFMDAMKAAGEDCAARVIAEAFTKAGFPARYINPGETGMLLSDDFGNAQVL
                     DESYPKLAALKQIKEIVIFPGFFGVTKGGEVATFPRGGSDITGAILAAAVKADVYENF
                     TDVDSVCSMDPRVIENPPAIDCMTYREMRELSYAGFGVFHDEAIVPAVRANIPICIKN
                     TNNPSAPGTMVVPKRAPGERTVVGISSAPGFCAIYIDKYMMNREIGFGRRLLQILEDE
                     KISFEHTPSGIDNISAILSSANLTPEKQEKVVARIKAELKPDAVLVEHGLSLIMVVGE
                     GMHYTPGLAAKATQAMAVAGVNLEMINQGAS"
BASE COUNT          372 a          411 c          440 g          316 t
ORIGIN      
        1 acaaaatcag catcgcgtcg atcatgcaga aagaacagaa cgcgggcgaa caggtaccgg
       61 tggtgtttct gacccacaag gcgctggaaa agaactatgc cgccgcaatg gaagagatcg
      121 aacggctgga tgtggtggac gggaaacccg tccgtatggc gatcgaggat tttgaataag
      181 agttaaccgc taatgttcac taatcttcgc tgatccggac ttaaattttg tgtccattag
      241 tggggattgg tggttcgaga ggatgaatga gatgaaagta gtgaaattcg gcgggacatc
      301 cgtcgccaac gcagaacaga tttcgaagat tgtgaaaatt gtcacggctg acgccgaccg
      361 ccggatcgtg gtggtttccg cgccgggtaa acggaataaa gccgacacca aggttaccga
      421 cctgctgatt gcgctggcaa ataccgtcat tgccggaaaa gattttgagg ccgggctgat
      481 ggcggtggtg aatcgttacg ctgaaattca gcgcgacctg aagctttcag aaaccgtcgt
      541 caaaaccatc gaagccgatc tgcgcggacg cattgcacgc cgcggcaaaa acgacgccca
      601 gttcatggac gccatgaaag ccgccggcga agactgcgcc gcccgcgtca ttgctgaagc
      661 gtttaccaaa gccggattcc ccgccagata tatcaacccc ggtgaaacgg gtatgctgct
      721 gagcgacgac ttcggtaacg cgcaggtcct cgacgagtcc tatccgaaac tggccgcgct
      781 gaaacagatc aaagaaatcg tgatttttcc cggctttttc ggcgtcacca aaggtggaga
      841 ggtcgcaaca ttcccgcgcg gcggttcgga tattaccggc gcgattctcg cggcggcggt
      901 caaagctgac gtctatgaaa actttaccga cgtggattca gtctgttcga tggatccgcg
      961 tgttattgaa aatccgcccg ctatcgactg catgacgtac cgcgaaatgc gcgaactttc
     1021 ctacgccggt ttcggcgttt tccacgacga agcgatcgtt cccgccgtgc gggccaacat
     1081 tccgatctgc atcaagaaca ccaataatcc gtcggctccc ggcaccatgg ttgtgccgaa
     1141 gcgcgcgccc ggcgaacgca cggtggtcgg catttccagc gctcccggct tctgcgcgat
     1201 ctatattgat aaatacatga tgaaccgcga aatcggtttc ggccgccgcc tgctccagat
     1261 tctggaagac gaaaaaattt ccttcgagca tacgccgtca gggatcgaca atatttccgc
     1321 aattctgtcg tcggccaatc tgactccgga gaaacaggaa aaggtggtcg cgcggattaa
     1381 agcagaactg aaacccgacg ccgtgctggt tgagcatggt ctctcgctca tcatggtggt
     1441 gggcgagggg atgcactata cgcccggtct ggcagccaaa gccacgcaag ccatggctgt
     1501 tgccggcgtg aatctggaaa tgatcaacca gggcgcgtc
//
LOCUS       DAOPZO010000218         1527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_128734_length_1527_cov_7.304348, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000218 DAOPZO010000000
VERSION     DAOPZO010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128734_length_1527_cov_7.304348"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1526
                     /locus_tag="PKI68_09330"
     CDS             <1..1526
                     /locus_tag="PKI68_09330"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNX53931.1"
                     /translation="LFSDGAVLQRGQDVPVWGTADKDCEVTVSFAGQQVSAKADERGL
                     WMVRLSPLEASSEGREMVISNGRDRVVVKNVVVGEVWVCSGQSNMDYTLGSMARPTKD
                     KIFQPLADFNQNEINTAEDPLFRQISVRQTVSPQEPRRDFSGSWLASSPKNNAGFSGT
                     GYFFGRELRRELNIPVGLVKCAWGGTCVEPWIPMDQYQTSDEMKAYYATTMAEKQAEH
                     VKWMDGSAKKEREEAVNAAKAQGAPAKKTKPPQDPLLQPTVPSGLYNGMIAPLVPYAV
                     KGVIWYQGESNQSYENSKYREHFEAVVNGWRLAWKQPRLDFYWCQLALYRAPAKQPVG
                     DDDGWVNVCYQQFQALALPNTGMAVLNDIGQPEDIHPRNKFDAGRRLAFWALNRSYGR
                     TDLVCSGPLYKSHVIQEDKVLITFDHVGSGLMTGKKVFMDPVVETKEPLHHFQIRGAD
                     GVWKWADAKIISRDTVEVRSPDIRQPVEVRYAWAMNPKGANLYNREGLPASLFKTAP"
BASE COUNT          328 a          352 c          501 g          346 t
ORIGIN      
        1 cgctgtttag tgacggagct gttttgcagc ggggacagga tgttccggtg tggggaacgg
       61 cggataagga ttgtgaagtg acggtgagct tcgccgggca gcaggtttcg gccaaggcgg
      121 atgaacgcgg gctctggatg gtgcgcctgt ctccgctgga agcctcgtcg gaagggcgcg
      181 agatggtgat cagtaacggg cgggatcgcg ttgtggtcaa aaacgtggtc gtcggcgaag
      241 tctgggtctg ttccgggcag tccaatatgg attacacgtt gggtagtatg gcgcgaccga
      301 ccaaagataa aatttttcag ccgctggcag actttaacca gaatgaaatc aatacggctg
      361 aggatccgct gtttcggcag atttcagtcc gtcaaacggt gtcgccacag gaacctcgcc
      421 gtgatttcag cggcagctgg ctggcttcgt cgccgaaaaa taatgccggg ttttcaggga
      481 ccggttattt tttcggtcgg gagttgcgcc gtgaattgaa tattccggtc gggctcgtca
      541 aatgtgcctg gggcggaacc tgtgtagagc cgtggattcc gatggatcag tatcagacgt
      601 ctgatgagat gaaggcctat tatgcaacga cgatggctga gaagcaggcc gagcatgtca
      661 agtggatgga tggatcggca aaaaaagaac gcgaagaggc tgtgaatgcc gccaaggctc
      721 aaggcgcacc ggcgaaaaaa acgaagcctc cgcaggatcc cttgctgcag ccgaccgtcc
      781 cgtcgggtct ctataacgga atgatcgctc cgctggttcc ttatgcggtt aagggtgtca
      841 tctggtatca gggcgaatcg aaccagtcct atgagaactc gaagtatcgc gagcatttcg
      901 aggcggtggt caatgggtgg cgtttagcgt ggaagcagcc ccggcttgac ttctactggt
      961 gtcagctggc cctgtatcgc gcgccggcca agcagccggt gggggatgat gatggttggg
     1021 tgaacgtctg ttaccagcag tttcaggcct tggcgctgcc caatacgggc atggccgtgt
     1081 tgaatgatat cgggcagccc gaagatattc accctcgcaa caagtttgat gcggggcggc
     1141 ggctggcttt ctgggcgctt aaccggtcgt atggacgaac ggatttggtc tgcagcggtc
     1201 cgctttacaa atcccatgtg atccaggagg acaaggttct cattaccttt gatcatgtcg
     1261 gttccgggct gatgaccggt aaaaaggtgt ttatggatcc ggtggttgaa acgaaggagc
     1321 cgctccatca ttttcagatt cggggcgcgg atggcgtgtg gaaatgggcc gatgcaaaga
     1381 ttatttcacg ggatacggtt gaggtcagaa gtcctgacat tcgccagccg gtggaagtgc
     1441 gctacgcctg ggcgatgaat ccgaagggtg ccaatcttta caaccgggaa ggtctgccgg
     1501 cgtcattatt taaaaccgcg ccgtaat
//
LOCUS       DAOPZO010000219         1521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pontiellaceae bacterium isolate AMR_MDS_1286
            NODE_129555_length_1521_cov_7.817190, whole genome shotgun
            sequence.
ACCESSION   DAOPZO010000219 DAOPZO010000000
VERSION     DAOPZO010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854491
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pontiellaceae bacterium (wastewater metagenome)
  ORGANISM  Pontiellaceae bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
            Pontiellaceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,867
            CDSs (total)                      :: 1,834
            Genes (coding)                    :: 1,829
            CDSs (with protein)               :: 1,829
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Pontiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129555_length_1521_cov_7.817190"
                     /isolate="AMR_MDS_1286"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2813032"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="PKI68_09335"
     CDS             complement(<1..1427)
                     /locus_tag="PKI68_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX53932.1"
                     /translation="MEKFRSLDELTPSHQKIARRIADLCSQMNGGFRLPVIQLCGADL
                     PSKKDIAAQVCAALGLNLCIMKAGNIPADPVQLDGLLRLWEREAFLTQCALLIDCSDN
                     DPADKLQSTALSRFIETAGGMILIAAAQRRRLEWRRQVTFDIGPPSPVERRSLWMTAF
                     DDKATRLHGQLEQLASQFQLSPRDIRASCEEALQAGTDDMESALWQACRIQARPRLER
                     LAQLIEPVASWDDLVLPALQKETLKAVAMHVRHRHQVYESWGFAAKSGRGLGISALFT
                     GGSGTGKTLAAEVLANDLKLDLFRIDLSQVVNKYIGETEKNLKEIFDAADAGGVILLF
                     DEADALFGKRSEVKDSHDRYANIEVSYLLQRMESYRGLAILTTNMKEALDKAFLRRIR
                     FIVQFPFPDAAQRSEIWRRIFPTATPTNRLDWERLSLLQVAGGNIRNIALSAAFLAAN
                     NNRPVDMTYIRQAVKQEYAKIEKQI"
BASE COUNT          332 a          439 c          403 g          347 t
ORIGIN      
        1 cttatttgtt tttcaatttt cgcatattcc tgtttgaccg cctgccggat gtacgtcatg
       61 tccacggggc ggttgttgtt cgccgccagg aaggccgcag acagcgctat attcctgata
      121 tttcctcccg ccacctgcag cagcgacaga cgttcccaat caagccggtt tgtcggtgtt
      181 gcagtcggga aaatgcgccg ccagatttcc gaccgctgtg cagcgtcggg gaagggaaac
      241 tggacgataa aacggatgcg ccgcagaaaa gctttgtcca gcgcctcctt catattggta
      301 gtcagaatcg ccagcccccg gtaggactcc atgcgctgca aaaggtagct gacttcaata
      361 ttggcgtagc ggtcgtggct gtccttgacc tcgctgcgct tcccgaagag cgcatcggct
      421 tcgtcaaaaa gtaaaataac cccgcccgca tcggcggcat caaaaatttc tttgagattt
      481 ttttcggttt ccccgatgta cttgttgacc acctggctca aatcaatgcg gaaaagatcg
      541 agtttcaagt cgttcgccag cacttcggcc gccagcgtct tccccgtgcc gctgcctccc
      601 gtgaataaag cgcttatgcc cagtccccgt cctgatttgg cggcaaagcc ccaggattca
      661 taaacctgat gtctatgccg gacatgcatg gccacggctt ttaaagtttc tttctgcagg
      721 gccggcagca ccagatcgtc ccacgaggca accggttcga tcaactgcgc caggcgttcc
      781 aggcgtggac gcgcctgtat ccggcaggct tgccagagag ccgattccat atcatccgtc
      841 ccggcctgca gcgcttcctc acaagacgcc cggatgtccc gggggctcaa ttgaaactgc
      901 gaggcaagtt gctccaattg accgtgaagt ctggttgcct tgtcgtcgaa cgccgtcatc
      961 cataaagagc gccgctccac cggggacggc ggtccaatat cgaaagtcac ctgacgacgc
     1021 cattccaggc gccttctctg cgctgcggca atcagaatca taccgccggc tgtctcgata
     1081 aaccggctca gggcggtgct ttgcaatttg tctgccggat cattgtcgga acagtcgata
     1141 agcagtgcac attgcgttag aaacgcctct cgttcccaga gtcgaagcaa cccgtccagt
     1201 tgaacgggat cggcaggtat attgccggct ttcattatgc agaggttgag tcccagggcg
     1261 gcgcagacct gcgcggcaat atcttttttc gacggtaaat cagcgccgca aagctgaatc
     1321 acgggcagtc gaaaaccgcc attcatttgc gagcaaagat ccgcaatgcg ccgggctatt
     1381 ttctggtgag aaggcgtaag ctcgtccaaa gaccggaatt tttccaccag agagctcaga
     1441 cgttcatcca gatactgaat gcctgtgaga taatgaagaa tccgctcatc aattttcagc
     1501 ggacaggatg tcagaacggg g
//
